LOCUS       DASPFR010000001         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_610790, whole genome shotgun sequence.
ACCESSION   DASPFR010000001 DASPFR010000000
VERSION     DASPFR010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610790"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1159
                     /locus_tag="VD737_00005"
     CDS             62..1159
                     /locus_tag="VD737_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HXW08971.1"
                     /translation="MARIDAFLKLGASQGCSDVHLAVGVPPMLRMNGELLPIKFRNLQ
                     EAELESYVTEILTTSHQEQFRKGHDIDFSYVSEDGNRFRVNVFHKDTGVGAVFRTIPS
                     DVPSLDSLNLPPVVKKFCDFHQGMVLVTGSTGTGKSTTLAAMIDHLNQTRRLNIISLE
                     DPIEFVHRSKQAQVIQRELHTHLPSFAEGVRAAMREDPDVILVGELRDADTVRWAMTA
                     AETGHLVLGTLHTTSAVKTIDRIVDALPAEEREQTKGFLSQSLLGVVTQILVRTPDLR
                     GRKAICEVMVMNRAIGKLITTEQTHMIPSQLQTGREVGMQTLDQALLASIQAREVDPE
                     DAFTYAVDKRPFARYVPEGTAILRADVTQGI"
     gene            1172..2293
                     /locus_tag="VD737_00010"
     CDS             1172..2293
                     /locus_tag="VD737_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HXW08972.1"
                     /translation="MATETPTATGTLTEAVPQHTPRKIDEFLREVLVKGGSDLHFVAG
                     DPPRIRLHGELQSLREAKLEKEAVREMLFEVMTRRAQSQLEQKDGADFAYTIPGVSRF
                     RVNVFRHIGGLGGVFRAIPSKALTLEQLNLPESVRSLCRVNNGLILVTGKTGSGKSTT
                     LAAMIDDINQREKGHILTIEDPIEFVHPRKNCLVSQREVGLHSPSFAEALHSALREDP
                     DIVLVGELRDLETMSIAVTAAEMGILVMGTLHTNGAAPTVDRIVNAFPADKQGHVRTM
                     LSTSLRGVISQQLIPRADKRGRVAALEILINTPAVSNLIRQGKLDQLENAMQSGAAHG
                     MRIMDSAIQELLEKGVINGRNAYEKAINKPRFEQFKDMG"
BASE COUNT          444 a          803 c          738 g          323 t
ORIGIN      
        1 ttccagagca tggacgagat gcggacggcc ctcgagaaat acctctgaac ggaaccgctg
       61 catggcacgc atcgacgcat tcctgaagct cggcgcctcg caaggctgct cggacgtgca
      121 cctcgccgtc ggcgtgccgc ccatgctgcg catgaacggc gaactgctgc caatcaagtt
      181 ccgcaacctg caggaggcgg aactcgagag ctacgtgacg gagatcctca cgacctcgca
      241 ccaggagcag ttccgcaagg ggcacgacat cgacttctcg tacgtgtccg aggacggcaa
      301 ccggttcagg gtcaacgtgt tccacaagga cacgggcgtc ggcgcggtgt tccgcacgat
      361 cccgagcgac gtgccgtcgc tcgactcgct gaacctgcca ccggtcgtca agaagttctg
      421 cgacttccac cagggcatgg tgctcgtgac cggatccacc ggcaccggca agtcgaccac
      481 gctcgcggcg atgatcgatc acctcaacca gacgcgccgg ctcaacatca tcagcctcga
      541 ggacccgatc gagttcgtcc accgcagcaa gcaggcgcag gtgatccagc gcgagctgca
      601 cacgcacctg ccctcgttcg ccgagggcgt gcgggccgcg atgcgcgagg acccggacgt
      661 gatcctggtc ggcgaactgc gcgacgccga cacggtgcgc tgggcgatga ccgcggccga
      721 gacggggcac ctcgtactcg gcacgcttca cacgaccagc gccgtcaaga cgatcgaccg
      781 catcgtcgac gcgctgccgg cagaggaacg cgagcagacc aagggcttcc tgtcgcagag
      841 cctgctcggc gtcgtcaccc agatcctggt gcgcacgccc gacctgcgcg ggcgcaaggc
      901 gatctgcgag gtcatggtca tgaatcgcgc gatcggcaag ctgatcacga ccgagcagac
      961 gcacatgatc ccgtcgcagc tgcagaccgg ccgcgaagtc ggcatgcaga cgctcgacca
     1021 ggcgctgctc gcgtcgatcc aggcgcgcga ggtcgacccg gaggacgcct tcacctacgc
     1081 ggtcgacaag cggccgtttg cgcgctacgt gcccgaaggc acggcgatcc tgcgcgccga
     1141 cgtcacccaa gggatttgag gcaccgacgc aatggccacc gaaaccccca cggcgaccgg
     1201 caccctgacc gaggcggtcc cgcagcacac gccgcgcaag atcgacgagt tcctgcgcga
     1261 ggtactcgtc aagggcggct ccgacctgca cttcgtcgcg ggcgacccgc cgcggatccg
     1321 cctgcacggc gagctgcagt cgctgcgcga ggccaagctc gagaaggaag ccgtccgcga
     1381 gatgctgttc gaggtcatga cacggcgcgc gcagtcgcag ctcgagcaga aggacggcgc
     1441 cgacttcgcc tacacgatcc cgggcgtgtc gcggtttcgc gtcaacgtgt tccgccacat
     1501 cggcgggctc ggcggcgtgt tccgcgcgat cccttccaag gcgctcacgc tcgagcagct
     1561 caacctgccc gagtccgtgc gcagcctctg tcgggtcaac aacgggctga tactcgtcac
     1621 ggggaagacc ggatccggca agtcgaccac gctcgcggcg atgatcgacg acatcaacca
     1681 gcgcgagaaa gggcacatcc tcacgatcga ggacccgatc gagttcgtgc acccgcgcaa
     1741 gaactgcctg gtcagccagc gcgaagtggg cctgcacagc ccgagcttcg ccgaggcgct
     1801 gcactcggcc ctgcgcgagg atcccgacat cgtgctggtc ggcgagttgc gcgatctcga
     1861 gaccatgagc atcgcggtga ccgcggccga gatgggcatc ctggtcatgg gcacgctgca
     1921 cacaaacggc gccgcgccca ccgtcgaccg catcgtcaac gcttttccgg cggacaagca
     1981 gggccacgtc cgtacgatgc tgtcgacgtc gctgcgcggc gtcatctcgc agcagctgat
     2041 cccccgggcc gacaagcgcg gccgcgtcgc agcgctcgag atcctgatca acacgccggc
     2101 cgtctcgaac ctgatccggc agggcaagct cgaccagctc gagaacgcga tgcagagcgg
     2161 cgccgcccac ggcatgcgca tcatggactc ggcgatccag gaactgctgg agaagggcgt
     2221 gatcaacggc cgcaacgcct acgaaaaggc gatcaacaag ccgcgcttcg aacagttcaa
     2281 ggacatgggc taggggacgc tacccttt
//
LOCUS       DASPFR010000002         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_733200, whole genome shotgun sequence.
ACCESSION   DASPFR010000002 DASPFR010000000
VERSION     DASPFR010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733200"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VD737_00015"
     CDS             complement(<1..391)
                     /locus_tag="VD737_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW08973.1"
                     /translation="MRRASFVLTMLGLVIAASATAAPVELLREVRHEAARRIVSGQLA
                     ATALDAASLALTLPDGRRVVARRTHLDRTSRSTTWTGEVIDMPGSRVALTSHRATVTG
                     VTDIDGQTYELRPDAADGRHLLVEVDTS"
     gene            complement(499..1236)
                     /locus_tag="VD737_00020"
     CDS             complement(499..1236)
                     /locus_tag="VD737_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXW08974.1"
                     /translation="MNLYDDETLGAYVDGELDDAERQAFERAVASDPELARRVETQRR
                     LRRALDDAFGPTLAEPVPDRLLRTAQGAVRDTVVSIETPRVRPTRWKAPHWWAIAASL
                     VVGAVLGRQALAPQADATLIATGPDGVVATGVLAKALSHQLAADRKGVVQVGLSFRAT
                     SGEYCRTFEIRGVRTAEAGLACRGPQAWHVQIVESMAPRPGAGGALRQASSGDLPESV
                     RRAVEASMAGDILDATAEAAARDAGWQ"
     gene            complement(1233..1790)
                     /locus_tag="VD737_00025"
     CDS             complement(1233..1790)
                     /locus_tag="VD737_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXW08975.1"
                     /translation="MYRRRPKNIFAVMEEDTRPARIADVEMSAAIVAALPRLRRFART
                     LCRNAHDADDLVQLAVERALRNVAQYRPDANPLHWLFGIVRHAWFDEVRARQRRRRIF
                     APAESGEDAPAADSTLEGIALQRALATLPDEQRLAVGLVLVEGLSYKEAADVLDVPIG
                     TLTSRLARARTALAAQLDPAVTEGR"
     gene            1860..>2648
                     /locus_tag="VD737_00030"
     CDS             1860..>2648
                     /locus_tag="VD737_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW08976.1"
                     /translation="MASLLTSPAPAQVTVPDVGVRLPEVTLPTPGIDVRSPALDAKLD
                     ARRLQVQARARSHPDRLALDPRGELIVRDELLAEPADEAALAAVLADGFTVRRRQELP
                     GLDVNLYVLQAPAGSSLPAALRRLRRLDPAGHYDYHHVLGEGGNAEATTVAVAPEAPP
                     VRAGASVQVQVGLVDGGVAATHEAFAGTSVVAHGCGGPIVPSAHGTAVASLLVETLGA
                     RSSRPPGHVQLLAADVYCGDAAGGAVERVIEALAWLAHRQVPVIN"
BASE COUNT          372 a          952 c          929 g          395 t
ORIGIN      
        1 gtgacgtgtc cacctccacc agcagatgcc ggccgtcggc cgcgtccggc cgcagttcat
       61 acgtctgccc gtcgatgtcg gtcacgccgg tgacagtcgc gcggtggctc gtcagcgcca
      121 cccggctgcc gggcatgtcg atcacctcgc cggtccaggt ggtcgagcgc gacgtacggt
      181 cgaggtgcgt ccgccgagcg acgaccctgc gaccatcggg cagcgtcagc gccaggctgg
      241 ccgcgtcgag tgccgtcgcc gcgagttgcc cggagacgat cctccgagcg gcttcatgtc
      301 gtacctcgcg cagcagttcg accggcgccg cggtggccga ggcggcgatg acgagaccga
      361 gcatcgtgag cacgaacgac gcgcgtcgca cgaggaagca tccggcggga gggccgggcg
      421 atcatcgatc gcctcgcccc gttgcggtgc ggactcgcca ccagtttggt ggcacttcgc
      481 ttcggcggat gcgcagggtc actgccagcc ggcgtcgcgg gccgcggcct ccgcagtcgc
      541 gtccaggatg tcgccggcca tcgatgcctc gaccgcgcga cggacgcttt ccggcaggtc
      601 gccgctcgag gcctggcgca gggctccgcc ggcccctggc cggggcgcca tcgactcgac
      661 gatctgcacg tgccacgcct gcggccctcg gcacgccagg ccggcttctg cggtgcgcac
      721 gccgcggatc tcgaacgtcc ggcagtactc acccgaggtg gcgcgaaagc tcaggccgac
      781 ctgcacgacg cctttgcgat cggcggcgag ctggtgcgac aacgccttcg ccagcacgcc
      841 cgtcgcaacc acgccgtcgg ggccggtcgc gatgagcgtg gcgtcggcct gcggcgcgag
      901 ggcctgccga cccaatacgg cgccgacgac caggctcgcc gcaatcgccc accagtgcgg
      961 tgccttccag cgggtcggtc ggacccgggg tgtctcgatc gataccacgg tgtctcgaac
     1021 ggctccctgc gcggttcgca gcagccgatc cggcaccggc tcggcgagcg tcggtccaaa
     1081 agcgtcgtcc agtgcgcgac ggagccgtcg ctgcgtttcg acacgacgcg ccagttcggg
     1141 gtcggaggcg acggcacgct cgaatgcctg gcgctccgcg tcgtccaact cgccgtcgac
     1201 ataggcgccg agcgtctcgt cgtcgtacag gttcatcgtc cctccgtcac ggccggatcg
     1261 agctgggcag cgagtgccgt tcgtgcgcgg gccagccggc tggtgagcgt accgatcggc
     1321 acgtcaagca cgtccgccgc ctccttgtac gaaagcccct cgaccaggac gaggcccacc
     1381 gccaggcgtt gctcgtccgg caacgtcgcg agcgctcgct gcaatgcgat gccctcgagc
     1441 gtcgaatccg cggccggagc gtcctcgccg gattccgccg gcgcgaagat ccgccggcgc
     1501 cgctggcggg ctcgcacctc gtcgaaccag gcgtgtcgca cgatgccgaa cagccagtgc
     1561 agcgggttcg cgtccggccg gtactgggcc acgttgcgca atgcgcgctc gaccgccagc
     1621 tgcacgaggt cgtcggcatc gtgcgcgttg cgacacaacg tccgcgcgaa ccggcgcagc
     1681 cgcggcagcg cagccacgat cgccgcgctc atctccacgt cggcaatacg cgcggggcgc
     1741 gtgtcttctt ccatgactgc aaaaatattc ttcggccgtc ggcggtacat taatgttgcc
     1801 acatgcaacc gaagcgcggc cgcgaccgaa accttcgaac gctgaacgcg ctgtgcgcaa
     1861 tggcgtccct gctgacgtcg cctgcgccgg cccaggtgac cgtgcccgac gtcggcgtgc
     1921 gcctgccgga ggtcactttg ccgacaccgg ggatcgatgt gcggagtccg gcgctggacg
     1981 cgaagctcga cgcgcggaga ctgcaggtgc aggcgcgcgc acggtcgcac ccggatcgac
     2041 tcgcactcga tccccgtggc gagctcatcg tgcgtgacga gttgctggca gagcctgccg
     2101 acgaagccgc gcttgccgcg gtcctcgcag acggctttac ggtgagacga cggcaggaac
     2161 tgccaggcct ggacgtgaat ctctacgtgc tccaggcgcc ggcgggttcg tctctgcccg
     2221 ccgcgctgcg acgcctgcgc aggctcgatc cggccggtca ttacgattat caccacgtgc
     2281 tgggcgaggg cggcaacgcg gaagcgacga ccgtcgctgt tgcaccggag gcgccgccgg
     2341 tccgcgccgg cgcgtccgtc caggtccaag tcggactcgt cgatgggggt gtcgcggcga
     2401 cacacgaggc gttcgccgga accagcgtcg tcgcccacgg atgcggcggt ccaatcgtgc
     2461 cgagtgcgca cggcacggcc gtcgcgtccc tgctcgtcga gacgctgggc gcgcgctcga
     2521 gccgtccgcc cggacacgtg cagctgctgg cagccgacgt gtattgcggc gatgcggcgg
     2581 gcggcgccgt ggagcgcgtg atcgaggcgc tcgcctggct tgcgcaccgg caggtgcccg
     2641 tgatcaac
//
LOCUS       DASPFR010000003         3663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1343606, whole genome shotgun sequence.
ACCESSION   DASPFR010000003 DASPFR010000000
VERSION     DASPFR010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343606"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VD737_00035"
     CDS             complement(<1..229)
                     /locus_tag="VD737_00035"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HXW08977.1"
                     /translation="MLYDLLELALADAALVFGSFVLLWGVSLAMRDSSLVDIWFAPCI
                     AAAAVLGVFVADGAEPRRVLIAVLAVIWATRL"
     gene            complement(261..746)
                     /locus_tag="VD737_00040"
     CDS             complement(261..746)
                     /locus_tag="VD737_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXW08978.1"
                     /translation="MPTATQLERLPLLARVRVPREWEDLNGHVNVQHHLGMYDMTSEA
                     MLRLLGVSTEWVHDQQVGLVDLEHHIWFQRELHVGDEVALHLRFTALNERRVQGVVFL
                     RNVASAEVASAIEFLSVAVDLAARRAIPVPSGVHERLVELLREHDALDWPAPRCGSIS
                     V"
     gene            876..1298
                     /locus_tag="VD737_00045"
     CDS             876..1298
                     /locus_tag="VD737_00045"
                     /inference="COORDINATES: protein motif:HMM:NF019473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXW08979.1"
                     /translation="MTDHAKTVHAYYAAWLADDLDTVMSLCTEDVVAVNVPIGPVKGK
                     PAVQKFFAKFQSRISDKRYDIHRVIADADSCVVEGVENYMRDGRQVSLPYMSTFLFRD
                     GLICEWRDYFDLKTLMDQLGLPLEAAQPAAPAQGANRA"
     gene            1295..2935
                     /locus_tag="VD737_00050"
     CDS             1295..2935
                     /locus_tag="VD737_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXW08980.1"
                     /translation="MTVEARPERRPPWFDPRVPSREDCVLRPMLDARAARHPERRFAL
                     FEDGTSWTYGECLAEVRATAAGLQRLGVRKGDRVLAWLPTGKTILQTWFAANYLGAVF
                     VPLNTAYRGAVLAHVVNAARARVLVAHPALAARLEGLELAHLTDVVVADEGNAGPAVP
                     GVRMHGASVLRGDGTSLDDGLEVDHWDEQSIIYTSGTTGLSKGVLSPYLQLYTTASIV
                     YGYLREGESILVNLPMFHVGGTSAVYIALIRGGGVHLVDGFSTTRFWDQVREGHCATT
                     SGLIGVMAAFLAREPARPDDRDNPLRCMTMFPVNEETVAFARRFGFDYLTGFNMTEVS
                     APLVTDINTSSYGSCGRPRSGVEARIVDEHDLDVPPGTIGELVLRSDTPWTMCIGYDG
                     QPQATAEAWRNGWFHTGDLFRQDERGEFYFVDRRKDAIRRRGENISSFEVENAVRQFP
                     DVEEAVAVGVQSEVAEEEVMVVVTSRAGRSVDPQALTEFLVPRLPYFMVPRYVRVVAE
                     IPKTETNKVRKVVFREQGITADTWDRERAGLRLRREKL"
     gene            complement(3012..>3663)
                     /locus_tag="VD737_00055"
     CDS             complement(3012..>3663)
                     /locus_tag="VD737_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HXW08981.1"
                     /translation="MANPPTTEALLEHFVRRSRVPLEEIKRHPHGAIFPDPQPLAEPK
                     EPGWPHRLQLAEATMLAQLAEVAARLPSGAGGVSLPLAERGADASGALELLLVSRREH
                     AVYNSVGHHLPALRKRRPYNPAYIHPEDARRAGLDDGAMVTIESTAGAVTAVLHHAPD
                     VRPGVVSMAHGFFASTDERRAASTSTLVDDTTDCEPITGLPRMSAIPVTLKKAVSG"
BASE COUNT          570 a         1266 c         1271 g          556 t
ORIGIN      
        1 cgagccgggt cgcccagatg acggccagca cggcgatcag cacgcgtcgc ggctccgcgc
       61 cgtcggcgac gaacacgccg aggacggccg ccgcggcgat gcacggggcg aaccagatgt
      121 ccacgaggct cgagtcgcgc atcgcgaggc tgacgcccca gagcagcacg aacgagccga
      181 agacgagggc ggcgtcggcc agcgcgagtt ccagcagatc gtagagcatc gagtcgggtt
      241 ccctggggca gtaattgggt tcagacggaa atgctgccgc agcgcggcgc cggccagtcg
      301 agcgcgtcgt gctcgcgcag cagttcgacg aggcgctcgt ggacgccgga cggcacggga
      361 atcgcgcgcc gggcggccag gtccacggcg accgacagga actcgatcgc gctcgcgacc
      421 tcggcggacg cgacgttgcg caggaacacc accccctgga cgcggcgctc gttgagagcc
      481 gtgaagcgca ggtgcagcgc gacctcgtcg ccgacgtgca gttcgcgctg gaaccagatg
      541 tggtgctcca ggtcgaccag gcccacctgc tggtcgtgga cccactccgt cgagactccc
      601 aacagccgca gcatcgcctc gctggtcatg tcgtacatgc cgaggtggtg ctggacgttg
      661 acgtggccgt tcaggtcctc ccattcgcgc ggcacgcgca cgcgcgcgag caggggcagt
      721 cgctcgagct gcgtcgccgt cggcatggtc actcgcaagg ccgccctcct gcccgctgcg
      781 ggcagtcact tgcacaactg tactgaggct cctatcctct atgtttccgc gccactgttc
      841 aagcgctgcc ggcttcgcag cggagggttg acgacatgac cgaccacgcc aagaccgtcc
      901 acgcctacta cgccgcctgg cttgccgacg acctcgacac cgtcatgtcg ctctgcaccg
      961 aggatgtcgt cgcggtcaac gtgccgatcg gcccggtcaa gggcaagccg gcggtccaga
     1021 agttcttcgc caagttccag agccgcatca gcgacaagcg ctacgacatc caccgcgtga
     1081 tcgccgacgc cgacagctgc gtggtcgaag gcgtcgagaa ctacatgcgc gacgggcgcc
     1141 aggtcagcct gccgtacatg tcgaccttcc tgttccgcga cggcctcatc tgcgagtggc
     1201 gcgactattt cgacctgaag acgctgatgg accagctcgg cctgccgctc gaggcggcgc
     1261 agccggcggc cccggcccag ggcgcgaacc gcgcgtgacc gtcgaggccc gtcccgagcg
     1321 gcggccgccg tggttcgatc cgcgggtgcc gtcgcgcgag gactgcgtgc tgcggccgat
     1381 gctcgatgcc cgggccgcgc gtcatcccga acggcggttc gcgctgttcg aggacggcac
     1441 gagctggacg tacggcgagt gcctggccga ggtccgtgcg accgccgcgg ggctgcagcg
     1501 cctgggcgtc cgcaagggcg atcgcgtgct cgcgtggctg ccgacgggca agacgatcct
     1561 ccagacctgg ttcgcggcga attacctcgg cgcggtgttc gtgccgctca acacggcgta
     1621 ccgcggcgcg gtgctcgctc acgtcgtcaa cgcggcgcgc gcccgcgtgc tcgtggcgca
     1681 ccctgcgctc gcggcacgcc tcgaagggct cgaactcgcc cacctcacgg acgtcgtcgt
     1741 cgccgacgaa ggcaacgccg ggccggccgt cccgggcgtg cgcatgcacg gcgcctccgt
     1801 gctgcggggc gatggcactt cactcgacga cggcctcgag gtcgatcact gggacgagca
     1861 gtcgatcatc tacacctcgg gtacgacggg tctgtccaag ggcgtgctgt cgccctacct
     1921 gcagctgtac acgaccgctt cgatcgtcta cggctacctg cgtgaaggcg agtcgatcct
     1981 cgtgaacctg ccgatgttcc acgtcggcgg caccagcgcg gtctacatcg cgctgatccg
     2041 gggcggcggc gtgcacctcg tcgacgggtt cagcacgacg cgcttctggg accaggtgcg
     2101 cgagggccac tgcgcgacca cgtcgggcct gatcggcgtc atggccgcct tcctggcgcg
     2161 cgaacccgcg cgaccggacg accgggacaa cccgctccgc tgcatgacga tgtttcccgt
     2221 caacgaggag acggtcgcct tcgcgcgtcg cttcggtttc gactacctga ccggtttcaa
     2281 catgaccgag gtgtcggcgc cgctcgtgac ggacatcaac acctcgtcgt acggctcctg
     2341 cgggcggccg cgcagcggcg tcgaggcccg catcgtcgac gagcacgatc tcgacgtgcc
     2401 gcccggcacc atcggcgagc tcgtgctgcg cagcgacacg ccctggacga tgtgcatcgg
     2461 ctacgacggg cagccgcagg cgaccgccga agcctggcgc aacggctggt tccacaccgg
     2521 cgacctgttc cgccaggacg agcgtgggga gttctacttc gtcgaccggc gcaaggacgc
     2581 gatccgtcgg cggggcgaga acatctcgtc gttcgaggtg gagaacgccg tgcggcagtt
     2641 tcccgacgtc gaagaggccg tggccgtcgg cgtgcagagc gaagtcgccg aggaagaagt
     2701 catggtcgtg gtcacgtcgc gggcgggccg ttccgtcgac ccgcaggccc tgaccgagtt
     2761 cctggtcccg cgcctgccct acttcatggt gccgcgatac gtacgcgtgg tcgcggaaat
     2821 cccgaagacg gaaaccaaca aggtccgcaa ggtcgtcttc cgagagcagg gcatcactgc
     2881 ggacacctgg gaccgcgagc gggccgggtt gcggctacgg cgggaaaaac tctgaagaag
     2941 cggttagcgg ttagtgaaca gcggttagtc ggagacggag gtcgatgatc tcccgaccct
     3001 cttactaacc gctaaccgct aaccgctttc ttcagcgtca ccggtatcgc gctcatccgc
     3061 ggcaggccgg tgatcggttc gcagtcggtc gtgtcgtcca ccagtgtcga cgtggacgcc
     3121 gcacggcgct cgtcggtcga cgcaaagaac ccgtgcgcca tcgacaccac gccgggtcga
     3181 acgtcgggcg cgtggtgcag caccgccgtg acggcgccgg ccgtcgactc gatcgtcacc
     3241 atggcgccgt cgtcgagacc cgcgcggcgg gcgtcctcgg gatgtatgta ggccgggttg
     3301 taggggcgac gcttgcgcag cgccggcagg tggtgcccga cggagttgta gaccgcgtgt
     3361 tcgcggcgac tcaccagcag caattccagt gcgccggacg cgtccgcgcc ccgctccgcc
     3421 agcggcaacg agaccccacc cgcgcctgac ggcagtcgcg ccgccacctc ggcgagctgg
     3481 gccagcatcg tggcctcggc gagttgcagg cggtgcggcc agcccggctc cttcggctcg
     3541 gcgagcggct gcgggtcggg aaagatcgcg ccgtgaggat gccgcttgat ctcctcgagc
     3601 ggcacgcgcg agcgccgcac gaagtgctcg agcagtgctt cggtcgtggg cggattggcc
     3661 atg
//
LOCUS       DASPFR010000004        11302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1587928, whole genome shotgun sequence.
ACCESSION   DASPFR010000004 DASPFR010000000
VERSION     DASPFR010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11302
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587928"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="VD737_00060"
     CDS             complement(<1..467)
                     /locus_tag="VD737_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW08982.1"
                     /translation="MILRGFVTQASYRVERDADGRRWPVVHVYGRAEDGATFLVRDHR
                     QRPHFYVRARDADLARGLGAAPASTDWRTFDGLPAARVERELPGDVPALRDALHAAGV
                     DTFEADVRFAYRYLIDRRIKASCVIEGTAEPGEDVTWIFDDPEVRPADAVSFEP"
     gene            complement(478..1185)
                     /locus_tag="VD737_00065"
     CDS             complement(478..1185)
                     /locus_tag="VD737_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HXW08983.1"
                     /translation="MAIWVGTSGYNYPEWKGSFYPEKLPAAQMLPYYADRLSTVEINY
                     TFYRTPNAKILAGWDVATPPGFRLTLKAPKRITHIARLRNCGESVRYFLDTAATLGPK
                     LGALFFQLPPNLKLDLALFDAFLEDLPAGTVAAFEFRHGSWLNDETYRRLASRNLALC
                     VADSETMATPVVVTADYAYFRLRDEGYTPADIERWAQTLRDRTAHCRDVYVYFKHEEA
                     GKGPQFARLLLDALGLP"
     gene            complement(1220..2050)
                     /locus_tag="VD737_00070"
     CDS             complement(1220..2050)
                     /locus_tag="VD737_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXW08984.1"
                     /translation="MESYSVRDVERVLRLPRSTIQGLIRSGFVQPTRGPRRQYRFSFQ
                     DLIVLRTARALSQAKVPSRRIRRALEDLRQHLPDTAPLSGLSIRAIGDRVVVREGQAQ
                     RQVEDGQYVLDLDVSVAQGELRVVEHREPPTAEPGEEPADWFRAGLELEESDPRAAVH
                     AYERAVEADPQQPAAWINWGRLLHEQGGKRDAESVYLRGLRACGPDPLLMFNLGVVLE
                     DMDRPGAALEAYQSALEEDPELADCHYNLARLYDTLGKPQHAIRHLGQYRRLVLGELG
                     "
     gene            complement(2068..2943)
                     /locus_tag="VD737_00075"
     CDS             complement(2068..2943)
                     /locus_tag="VD737_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HXW08985.1"
                     /translation="MAAPRSIGSLTVSFGLVAIPVKLYSATQSTGQISFNMLHKDCGS
                     RLKQQYVCLKEGVVVERDDIVKGYEFAKDQYVAFTPEEIKALDEVGTHAVEISEFVPI
                     ESIDPVYFDKTYYLSPDKGASKPYALLTEALKETKRCAIGRWAARGKAYIVMLRPVDG
                     VLAMQQLHFGTEVRPAREVEVPKTEVKPAELKLARQLIDSQQADLFDPTSYVDEVRGR
                     VEAAIQKKVEGQEISVSEAPVASSGKVIDLMEALRASLEKTEAARASTSKLGPRKSAR
                     RVEEPKPAARKSAKR"
     gene            complement(2962..5667)
                     /gene="ligD"
                     /locus_tag="VD737_00080"
     CDS             complement(2962..5667)
                     /gene="ligD"
                     /locus_tag="VD737_00080"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HXW08986.1"
                     /translation="MKRTLDEYAAKRKFARTPEPGPKVTRARRGPLLFVVQKHAARRL
                     HYDFRLEVDGVLKSWAVPKGPSLDPADKRLAVPTEDHPFDYASFEGVIPEKEYGAGNV
                     IVWDSGVYSPDEGGAYAFDDRRTAEERVRRELAQGKLSFFLRGEKLKGSFALVRTSAA
                     GQWLLIKHQDRFAHQNDVLALARSVLSDRTVEEDPPARTSQVDAARLAPTGPAERMPE
                     TLSPMLAEIGEPPRPGEAWAYEPKVDGYRVLAFVRDGRVRLQSRRGLDLTVAFPEVVT
                     ALAEPAAGDMVLDGELLALDATGRPSFNALQNRAGLKQPREITEAQRHTSVVYMCFDL
                     LHFAGVNLRGSPYAERRRYLSQCLLPSRHVQLVHVSGDPDALYAAALENGFEGIVAKR
                     KDSTYQPGRRSTAWLKKKAVESSEFVVGGYTRGKGARESLGALLLGQWRGDELHYVGH
                     VGSGLDERSLEALGERLAARASKTSPFAAKPPLHRPTQWLEPELVAEVSYAGWTPDGY
                     LRAPVFLRLRDDVAPRSVRSRPRPRASPVTRQADPSDVIQDVVAQLEGRQNRVDLKVG
                     DARLRLTNLDRPYWPGDARQKRPAITKRDLLKYLAQVSPYMLPHLAQRPLTMIRMPEG
                     IHGERFFQKHWTQQRPEFVESVTVYSGHKNEPHEYLLANNLPSLLWFGQVGTLEFHVW
                     HSRAEPGPDSKSRLTDYASSLQALEASVLNYPDFLVFDIDPYIYSGKEAKGAEPELNR
                     KGYSTGRRVAFWLRDLLREMSLEAIVKTSGKTGLHVFVPIERTVTFDEARALCELVGR
                     HLMREHPKEITMEWSVEKRTGKIFYDYNMNVRGKTLNVAYSPRGVPGAPVSMPLTWEE
                     LEAAEPTQFTLLNAPARLDRTGDRWHDVLSTKQSLARAFASSSAK"
     gene            5755..7227
                     /locus_tag="VD737_00085"
     CDS             5755..7227
                     /locus_tag="VD737_00085"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXW08987.1"
                     /translation="MRFQNDPGGHRLPVKLDTTSNGEYAPIPLEPVHREARAHAMQTA
                     TTNARRLGLQRREFLVSACGVATSLLGMNAAYARAGRTGGYFEVPTLAALEEPLARAA
                     VSGDEFIFDVQGHFVNPTGAWLRELPAGARPMRSFTADARCEPHAAGPGELDYLRCIG
                     PDQFVKDVFMDSDTDLMVLSFVPSTPEGEPLKIEEAEATARVVERLEGTHRLYIHGRV
                     NPNQPGDLERMDLLAERHKVSAWKTYTQWGPEQKGFWMDDEPGLALIEKARKLGIRNI
                     AIHKGLSFGPQSYEHSTCRDIGRVAKRFPDVNFLIYHSGFVANAKEGPYDPKRTDGVD
                     ALVTSLLENGVKPGSNVYAELGSTWRLCMRDPDTAAHTLGKLIRHCGESNVLWGTDSI
                     WYGSPQDQIQAFRTFQISPELRERHGYPEITPQLRAKIFGLNALRIYPVPRDVLGEHL
                     ERDRVATARAEYRERPDPAFVTYGPKNRREFLNLLALGGG"
     gene            complement(7258..7971)
                     /locus_tag="VD737_00090"
     CDS             complement(7258..7971)
                     /locus_tag="VD737_00090"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXW08988.1"
                     /translation="MALVVGAGDATGAAIARRFAREGYVACLARRSAGKLQPVVEAIR
                     AAGGQAHGFGTDARNEAAVAELIERIESDVGPIEVFAFNIGANVPSAVLDETPRKFQK
                     IWEMACFSAFLTGQAVARRMVARGRGTMLFTGATASTRGSANFAAFAVAKHGLRALAQ
                     SMARELGPLNVHVAHVIVDGAIDTAFIRENFPDKYAERAQDGILQPDHIADAYWYLHR
                     QPRDAWTFELDLRPWRERW"
     gene            8362..8877
                     /gene="nrfH"
                     /locus_tag="VD737_00095"
     CDS             8362..8877
                     /gene="nrfH"
                     /locus_tag="VD737_00095"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HXW08989.1"
                     /translation="MLAVLVGVLLGLGAYTFLYAHGISYLSTDPAACANCHIMQSQYE
                     SWQKSSHHAVARCVDCHLPHDFIGKYIAKAENGWHHSKGFTLQDFHEPIMIKSKNALI
                     LQRNCLACHEGIAHGLVEGVNGEPDEVQCVHCHGDVGHGETTGLGGPEREQEIEAADR
                     AAATASQGKKT"
     gene            8874..10337
                     /locus_tag="VD737_00100"
     CDS             8874..10337
                     /locus_tag="VD737_00100"
                     /inference="COORDINATES: protein motif:HMM:NF014398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HXW08990.1"
                     /translation="MTTHVKQQGLSLPVVLAVALVVAAITAGVAALLVNINERKTEAR
                     TTSVRLVEVTEDTTDPAQWGVNWPKQYDSYQRTAISTRTRFGGHGGSEALPEQKIERD
                     PWLKRMFLGYAFSIDYRDRRGHAYMLTDQEQTLRLTKPQSGSCLHCHASVMPLYRKLG
                     NGDAKAGLDATHKMTYQETNAMLKEVGGAHPVSCVDCHDPQTMKLRVTRPGFFAGIQA
                     LAESDAPVPHLPSIEQWREGGRAKPYDPNVDGTRNEMRSYVCGQCHVEYYCSTKMPLT
                     FPWNKGLRAGQVEQAWNEMKFPDGEQFFDYKHAESGARVLKAQHPEFELWSQGVHARS
                     GVSCSDCHMPYMREGATKVSDHWVRSPLLNVNRACQTCHHFSEEELKQRVDVIQTRNF
                     ELMQRGGDAIVALLDAIQAAKAAGATEEQLAPALEFQRKSQWRLDFIAAENSMGFHAP
                     QEAAMVLGEAIDYARQGEVAARNWSGPAKPVQTAAAN"
     gene            10340..10711
                     /locus_tag="VD737_00105"
     CDS             10340..10711
                     /locus_tag="VD737_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HXW08991.1"
                     /translation="MSVPAIECPRCAATLARVRMGGIDTDVCEACGGLWLDRYELARF
                     DRPEFAFGDALVAHLQQFPAQLLEHTVRLRCPHHRDVTMLRRPYAPHARVEIDECPQC
                     GGVWLDADELASIRDARSARS"
     gene            complement(10739..>11302)
                     /locus_tag="VD737_00110"
     CDS             complement(10739..>11302)
                     /locus_tag="VD737_00110"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HXW08992.1"
                     /translation="SALEGARRNVVYFPGSTLGNWAPQDAVALLRRFARIAGPGGVVL
                     LGLDAVKPIEILERAYDDAAGVTAAFNLNALEHINRVTGARIDTNAFRHFAPWVAAHE
                     RIEMRLVARRDVAFDVGAARVRLRRGEYLLTEFSHKYTAESAARIAAEAGLVVQQAWF
                     DPQRWFGVWLLAPGGGAYAAPTDAPLT"
BASE COUNT         1768 a         3902 c         3890 g         1742 t
ORIGIN      
        1 ggctcgaaac tcaccgcgtc cgccggccgc acctccgggt cgtcgaaaat ccaggtcacg
       61 tcctcgccgg gttcggccgt gccctcgatc acgcagctcg ccttgatgcg gcggtcgatc
      121 aggtagcggt acgcaaagcg aacgtcggcc tcgaaggtgt cgacgccggc ggcgtgcagc
      181 gcgtcgcgca gcgcgggcac gtcgccgggt agctcccgct ccacgcgcgc ggccggcagt
      241 ccgtcgaacg tccgccagtc cgtcgacgcg ggcgcggcgc cgagcccgcg ggccaggtcc
      301 gcgtcgcggg cgcggacgta gaagtgtgga cgctggcggt ggtcgcggac cagaaacgtc
      361 gcaccgtctt cggcgcgtcc gtagacgtgg acgacgggcc accggcgtcc gtccgcatcg
      421 cgttcgacgc ggtaactcgc ctgcgtgacg aagccgcgca ggatcacgcg tgtcgactca
      481 cggcagtccc agtgcgtcga gcagcaggcg tgcgaactgc ggccccttgc cggcctcttc
      541 gtgcttgaag tacacgtaca cgtccctgca gtgggcggtc cggtcgcgca gcgtctgcgc
      601 ccagcgctcg atgtccgcag gggtatagcc ttcgtcgcgc aggcggaagt acgcgtagtc
      661 ggcggtgacg acgacgggcg tcgccatggt ctcgctgtcg gccacgcaca aggccaggtt
      721 gcgcgatgcg aggcgccgat aggtctcgtc gttgagccac gagccgtgcc ggaactcgaa
      781 cgcggcgacg gtgcccgccg gcaagtcttc gaggaatgcg tcgaacagcg cgaggtccag
      841 cttgaggttc ggcggcaact ggaagaacag tgccccgagc ttgggcccga gggtggccgc
      901 cgtgtcgagg aaatatcgca ccgactcgcc gcagttcctg aggcgtgcga tgtgcgtgat
      961 gcgcttgggg gccttgagcg tcagccggaa cccgggcggt gtcgcgacgt cccatccagc
     1021 gagaatcttc gcgttgggcg tgcggtagaa cgtgtaattg atctcgaccg tggagagccg
     1081 atccgcgtag tacggcagca tctgcgcggc cggcagcttc tccggataga agctgccctt
     1141 ccattccgga tagttgtatc cggacgttcc cacccagatg gccatccttg cctcctggca
     1201 acgtcagccg ataggcagct cagccgagtt cgccgagcac gagccggcgg tactgcccga
     1261 ggtgccggat cgcgtgctgc ggcttgccga gcgtgtcgta cagccgggcg aggttgtagt
     1321 ggcagtcggc gagctccggg tcttcctcca gcgccgactg gtacgcctcg agggccgcgc
     1381 cgggccggtc catgtcctcg agcaccacgc cgaggttgaa catcagcaac gggtccggcc
     1441 cgcaggcccg cagcccgcgc agatacaccg actccgcatc gcgcttgccg ccctgttcgt
     1501 gcagcagtcg cccccagttg atccaggcgg ccggctgctg cggatcggct tcgacggcgc
     1561 gttcgtaggc gtggaccgcc gcgcgagggt ccgactcctc gagttcgagc cccgcgcgaa
     1621 accagtcggc cggctcctcg ccgggctccg ccgtgggggg ctcacggtgc tccacgaccc
     1681 gcagttcacc ctgcgcgacg ctgacgtcga ggtcgagcac gtactgcccg tcctcgacct
     1741 ggcgctgcgc ctggccttcg cggacgacga cgcggtcgcc gatcgcgcgg atgctgagcc
     1801 cggacagcgg ggcggtgtcg ggcaggtgct gccgcaggtc ctcgagcgcg cgacggatcc
     1861 gacggctcgg caccttggcc tgtgacagtg cgcgcgcggt gcgcagcacg atcaggtcct
     1921 ggaacgaaaa gcgatactgg cgccgcggac cgcgggtcgg ctgcacgaac ccgctgcgga
     1981 tcaggccctg gatggtgctg cgaggcaggc gcaggactcg ttcgacgtcc cgcacgctgt
     2041 acgattccat cgtggacctg cgcagcgtta ccgcttggcg gacttgcgcg ccgcgggctt
     2101 aggctcttcg acgcgcctgg ccgacttgcg cgggccgagc ttcgacgtcg aggcgcgtgc
     2161 ggcctcggtc ttctcgaggc tcgcgcgcag cgcctccatg aggtcgatga ccttgccgct
     2221 gctggcgacg ggcgcctccg acaccgagat ttcctggccc tcgaccttct tctggatggc
     2281 ggcctcgacg cgtccgcgca cctcgtcgac gtacgaggtc gggtcgaaca ggtcggcctg
     2341 ctgcgagtcg atcagctgcc gggcgagctt gagctcggcg ggcttcacct cggtcttcgg
     2401 cacctcgacc tcgcgcgcgg gccggacttc ggtcccgaag tgcagctgct gcatcgcgag
     2461 cacgccgtcg accggccgca gcatcacgat gtaggccttg ccgcgcgccg cccagcgccc
     2521 gatcgcgcag cgcttggtct ccttgagtgc ttcggtgagc agtgcgtacg gtttcgacgc
     2581 gcccttgtcc ggcgagaggt agtacgtctt gtcgaagtac accggatcga tcgactcgat
     2641 cggcacgaac tccgaaatct cgacggcgtg ggtgcccact tcgtcgagcg ccttgatctc
     2701 ctcgggcgtg aaggccacgt actggtcctt cgcgaactcg tagcccttga cgatgtcgtc
     2761 gcgctcgacg acgacgcctt ccttcaggca cacgtactgc tgcttcaggc gtgagccgca
     2821 atccttgtgc agcatgttga aggagatctg tccggtcgac tgggtcgccg agtaaagctt
     2881 gaccgggatg gcgaccaggc cgaacgaaac ggtgagcgag ccaatggagc gtggtgcggc
     2941 catgcgagcc cctctgcgtg gttactttgc ggagctgctt gcaaacgctc gcgcaaggct
     3001 ttgttttgtc gacaatacat catgccaccg gtccccggtc ctgtccaaac gtgccggggc
     3061 attcagaagc gtgaactggg tcggctcggc ggcctccagc tcctcccagg tcagcggcat
     3121 cgacaccggg gccccgggga ccccccgggg ggagtacgcg acgttcaggg tcttgccccg
     3181 cacgttcatg ttgtagtcgt agaagatctt gccggtgcgc ttctcgacgg accactccat
     3241 cgtgatctcc ttcgggtgct cgcgcatcag gtggcgcccg accagttcgc agagggcgcg
     3301 ggcctcgtcg aaggtcaccg tgcgctcgat cggcacgaac acgtgcaggc cggtcttgcc
     3361 cgaggtcttg acgatcgcct ccagcgacat ctccctgagc aggtcgcgga gccagaaggc
     3421 aacgcgccgg ccggtcgagt agcccttgcg attgagttcg ggttccgcgc ccttggcctc
     3481 cttcccggaa tagatgtatg gatcgatgtc gaacaccagg aagtccggat agttcagcac
     3541 cgaggcctcg agcgcctgga gcgagctcgc gtagtccgtg agccgtgact tcgagtccgg
     3601 ccccggctcg gcgcgcgagt gccagacgtg gaactcgagc gtgccgacct gcccgaacca
     3661 cagcagcgac ggcaggttgt tggcgagcag gtattcgtgc ggctcgttct tgtggcccga
     3721 gtagacggtc acgctctcga cgaactcggg gcgctgctgg gtccagtgct tctggaagaa
     3781 gcgttcgccg tggatgccct ccggcatgcg gatcatcgtg agcggccgtt gcgccaggtg
     3841 cggcagcatg tagggcgaca cctgcgcgag gtacttgagc aggtcgcgct tggtgatggc
     3901 gggccgcttc tggcgcgcgt cgccgggcca gtatggccgg tcgaggttcg tcaggcggag
     3961 gcgggcgtcg ccgaccttga ggtcgacgcg gttctgcctg ccctcgagtt gcgcgacgac
     4021 gtcctggatg acgtccgacg ggtccgcttg cctggttact ggactggcac gcgggcgcgg
     4081 gcgactgcgc acgctgcgcg gcgcgacgtc gtcgcgcagg cgcaggaaca ccggcgcacg
     4141 caggtagccg tcgggcgtcc accccgcgta gctgacttcc gcgacgagct ctggctcgag
     4201 ccactgcgtc ggccggtgca acggcggctt ggccgcgaac ggcgacgtct tcgacgcgcg
     4261 cgccgcgagc cgctcgccga gcgcctcgag gctgcgttcg tcgagccccg agccgacgtg
     4321 accgacatag tgcagttcgt cgccccgcca ctggccgagc agcagggcgc cgagcgattc
     4381 gcgggcgccc ttgccgcggg tgtagccgcc gacgacgaat tccgagcttt cgaccgcctt
     4441 cttcttgagc caggccgtcg accggcgccc cggctggtac gtgctgtcct tgcgcttggc
     4501 gacgatgccc tcgaagccgt tctcgagcgc ggcggcgtag agcgcgtcgg ggtcgcccga
     4561 gacgtgaacg agttgcacgt gccgcgacgg cagcaggcac tgcgaaaggt accgccgacg
     4621 ctcggcgtag gggctgccgc gcaggttcac gccggcgaag tgcagcaggt cgaagcacat
     4681 gtacacgacg ctcgtgtggc gctgtgcctc ggtgatttcg cgcggctgct tcaagccggc
     4741 gcgattctgc agcgcgttga acgacggccg gcctgtggcg tcgagcgcga gcaactcgcc
     4801 gtcgagcacc atgtcaccgg cggcgggttc ggcgagcgcc gtgacgactt ccgggaacgc
     4861 gaccgtcagg tcgagcccgc gccgcgactg caggcgcacg cggccgtcgc gaacgaacgc
     4921 gaggacccga tagccgtcga ccttgggctc gtacgcccag gcctcgcccg ggcgcggcgg
     4981 ctcgccgatt tccgcgagca tcgggctcag cgtctcgggc atgcgctcgg caggaccggt
     5041 cggcgcgagg cgagcggcgt cgacctgaga cgtgcgtgct ggcggatcct cttccacggt
     5101 gcggtccgag agcaccgagc gggccagcgc gagcacgtcg ttctggtgcg cgaaccggtc
     5161 ctggtgcttg atcagcagcc actggcccgc ggcggacgtg cgcacgagcg caaacgaccc
     5221 cttgagcttc tctccccgca agaagaagct gagcttgccc tgggccagct ccctgcgcac
     5281 gcgctcctcc gccgtgcgac ggtcgtcgaa cgcgtacgcg ccgccctcgt cgggcgaata
     5341 cacgccactg tcccagacga tcacgttgcc cgcgccgtat tccttttcgg ggatgacccc
     5401 ctcgaacgac gcgtagtcga aagggtggtc ctcggtcggg acggcgaggc gcttgtcggc
     5461 ggggtcgagg gacggaccct tgggcaccgc ccacgacttc aggacgccgt cgacctcgag
     5521 ccggaagtca tagtgcagtc gccgggccgc gtgcttctgc acgacgaaca gcagcggtcc
     5581 gcgccgtgcg cgcgtgacct tggggcccgg ctcgggcgtg cgggcgaact tgcgtttggc
     5641 cgcatattcg tcgagggtgc gcttcatcgc ggggcaacat taccgcaagg cgggcaatga
     5701 ccgtctgcgc cgtcccggag ccggggtcag gacgtgccgc aaggaggatc gcgcatgagg
     5761 ttccagaacg acccgggcgg ccaccgcctc ccggtcaagc tcgacacgac gagcaacggc
     5821 gagtacgcgc ccatccctct cgaaccagtg caccgcgagg cgcgtgcgca cgccatgcag
     5881 acggccacga cgaacgcgcg ccggctcggc ctgcagcgcc gcgagttcct ggtgtccgcc
     5941 tgcggcgtcg ccacctcgct gctcggcatg aatgcggcgt atgcgcgggc gggccgcacg
     6001 ggcggttact tcgaagtccc gaccctcgct gcgctcgagg agcccctggc gcgggccgcc
     6061 gtctcgggcg acgagttcat cttcgacgta cagggtcact tcgtgaaccc gaccggcgcg
     6121 tggctccgcg agctgccggc cggcgcgcga ccgatgcgca gcttcaccgc cgatgcccgc
     6181 tgcgaaccgc acgccgccgg gccgggcgaa ctcgactacc tgcgctgcat cggcccggac
     6241 cagttcgtga aggacgtctt catggactcc gacaccgacc tgatggtgct gtccttcgtg
     6301 ccttcgactc ccgagggcga gcccctcaag atcgaggagg ccgaggcgac cgcgcgggtc
     6361 gtcgagcggc tcgagggcac ccaccggctc tacatccacg gacgcgtcaa cccgaaccag
     6421 cccggcgatc tcgaacgcat ggacctgctg gcggaacgtc acaaggtgtc ggcctggaaa
     6481 acctacacgc agtgggggcc ggagcagaag ggattctgga tggacgacga gccgggcctc
     6541 gcgctgatcg agaaggcgcg caagctcggc atccgcaaca tcgcgatcca caaggggctg
     6601 tcgttcggcc cgcagtcgta cgagcactcg acctgtcgcg acatcggtcg cgtcgcgaag
     6661 cgtttccccg acgtcaactt cctgatctac cactccggct tcgtcgccaa cgccaaggaa
     6721 ggcccgtacg acccgaagcg caccgacggc gtcgacgcgc tcgtcacgag cctgctcgag
     6781 aacggagtca agccgggcag caacgtctat gcggaactcg gttcgacctg gcgactgtgc
     6841 atgcgcgatc ccgacacggc ggcgcacacg ctcggcaagc tcatcaggca ctgcggcgag
     6901 tcgaacgtgc tctggggaac cgactcgatc tggtacggct cgccgcagga ccagatccag
     6961 gcgttccgca cgttccagat ctcaccggag ctgcgcgagc gtcacggtta cccggagatc
     7021 acgccgcagc tgcgtgcgaa gatcttcggc ctgaacgcgc tgcggattta cccggtgccg
     7081 agggacgtcc tcggcgaaca cctcgaacgc gaccgcgtcg ccacggcgcg cgccgagtac
     7141 cgcgagcggc cagaccccgc gttcgtcacc tacgggccga agaaccgccg cgaattcctg
     7201 aacctgctgg cgctcggcgg cggctgagcg cggcgtcgtg cgatgcggcg cggcgcgtca
     7261 ccagcgttcg cgccacggcc gcaggtcgag ttcgaacgtc cacgcgtcgc gcggctggcg
     7321 gtgcaggtac cagtacgcgt cggcgatgtg gtcgggctgc aggatcccgt cctgggcgcg
     7381 ctcggcatac ttgtccggaa agttctcgcg tatgaaggcc gtgtcgatcg caccgtccac
     7441 gatcacgtgg gcgacgtgga cgttcagcgg gcccagttcg cgcgccatgc tctgcgccag
     7501 cgcgcgcagg ccgtgcttgg cgaccgcgaa cgccgcgaag ttggccgagc cacgcgtgga
     7561 cgccgtggcg ccggtgaaca gcatcgtgcc acggccgcgc gcgaccatgc gtcgcgcgac
     7621 ggcctgcccg gtcaggaacg ccgagaagca cgccatctcc cagatcttct ggaacttgcg
     7681 cggcgtctcg tcgaggaccg cgctcggcac gttggcgccg atgttgaacg cgaacacttc
     7741 gatcggcccg acgtcggact cgatgcgctc gatgagctcc gccaccgccg cctcgttgcg
     7801 tgcgtcggtg ccgaagccgt gcgcctggcc gcccgccgcg cggatcgcct cgaccacggg
     7861 ctgcagcttg cccgccgatc gacgcgcgag gcacgcgacg tacccttcac gggcgaagcg
     7921 tcgcgcgatg gccgcgccgg tcgcatcccc ggcgccgacg accagtgcca cgcccgctgc
     7981 agtcgtgtcc gtcactgtcg tcgtcactgc tgacgccctc gctgcacgct cgcacctgca
     8041 atcgttgcag cgtagcgaac gctgggcgca cgcgccacac gcgggatcac acgtcggagc
     8101 atcgtcgcgg ctgccttcgt cgcatgaatc gttctaagat cgacgctgcg tcctgccagt
     8161 cgagacggcg tgtttcggcg gcagggtgcg ccgcgccgtc ccgtcattga tctccatcaa
     8221 tcgggcggta cgcaataaca cgcactactg cggccattgc tgcaccgcga gaattgcccg
     8281 actggacccg aggagcccca ggtggggagc cgagacgcag acagaaagac cgcaagtgcg
     8341 ctgaccggac cggccctcct cgtgctggcc gtgctcgtgg gcgtgctgct gggactcggc
     8401 gcctacacgt ttctctacgc ccacggcatc tcgtacctga gcacggaccc cgcggcctgc
     8461 gccaactgcc acatcatgca gtcgcagtac gaaagctggc agaagagctc gcatcacgcg
     8521 gtcgccaggt gcgtcgactg ccacctgccg cacgacttca tcggcaagta catcgccaag
     8581 gccgaaaacg gctggcacca ctccaaggga ttcacgctgc aggacttcca cgagccgatc
     8641 atgatcaagt cgaagaacgc cttgatcctg cagcgcaact gcctcgcctg ccacgagggc
     8701 atcgcccacg ggctcgtcga gggcgtcaac ggcgaaccgg acgaggtgca gtgcgtccac
     8761 tgccacggcg atgtgggcca cggcgagacg accgggctcg ggggtcccga gcgcgagcag
     8821 gaaatcgagg cggccgaccg cgcggccgcc accgccagcc agggtaagaa gacatgacta
     8881 cacacgtgaa acagcaaggt ttgtcgctgc cggtcgtcct tgcggtggcg ctcgtcgtcg
     8941 ccgccattac cgcgggcgtc gcggccttgc tcgtcaacat caacgagcgc aagacggaag
     9001 cgaggaccac gtcggtccgg ctcgtcgaag tgacggaaga cacgaccgat ccggcgcagt
     9061 ggggcgtcaa ctggccgaag cagtacgact cgtaccagcg cacggcgatc tcgacgcgca
     9121 cgcgattcgg cggtcacggc ggcagcgagg cgctgccgga gcagaagatc gagcgcgacc
     9181 cgtggctcaa gcgcatgttc ctgggctatg cgttctcgat cgactaccgc gaccgccgcg
     9241 ggcacgccta catgctcacc gaccaggagc agacgctccg tctgaccaag ccgcagtccg
     9301 gctcgtgcct gcactgccac gcgtcggtga tgccgctgta ccgcaagctc ggcaacggcg
     9361 acgcgaaggc ggggctggac gccacgcaca agatgaccta ccaggagacc aacgccatgc
     9421 tcaaggaggt cggcggcgcg cacccggtct cctgcgtcga ttgccacgat ccgcagacga
     9481 tgaagctgcg cgtcacgcgc cccgggttct tcgccggcat ccaggcactg gcggaaagcg
     9541 atgcgcccgt gccgcacctg ccctcgatcg agcagtggcg cgaaggcggc cgcgccaagc
     9601 cgtacgaccc gaacgtcgac ggcacgcgca acgaaatgcg ctcctacgtc tgcggccagt
     9661 gccacgtcga gtattactgc tcgaccaaga tgccgctcac gttcccgtgg aacaagggcc
     9721 tgcgcgccgg ccaggtcgag caggcgtgga acgagatgaa gttcccggac ggcgagcagt
     9781 tcttcgacta caagcacgcc gagagcggcg ccagggtact caaggcgcag caccccgagt
     9841 tcgaactctg gagccagggc gtgcacgccc gcagcggcgt ctcgtgctcc gactgccaca
     9901 tgccgtacat gcgcgagggc gccaccaagg tgtccgacca ctgggtgcgc agcccgctgc
     9961 tcaacgtcaa tcgcgcgtgc cagacctgcc accacttctc cgaggaagaa ctcaagcagc
    10021 gcgtcgacgt gatccagacg cgcaacttcg agctgatgca gcggggcggc gacgcgatcg
    10081 tcgcgctgct cgacgccatc caggccgcga aggcggccgg tgccaccgag gagcagttgg
    10141 cgccggcgct ggagttccag cgcaagtcgc agtggcggct cgatttcatc gcggcggaga
    10201 actcgatggg gttccacgcg ccgcaggagg ccgccatggt gctgggcgag gcgatcgact
    10261 acgcgcgcca gggtgaagtc gcagctcgca actggagcgg cccggcgaaa ccggtgcaga
    10321 cggctgccgc gaattagccg tgtccgtgcc ggcgatcgag tgcccacgct gcgcggcgac
    10381 gctcgcccgc gtcaggatgg gcgggatcga taccgacgtc tgcgaggcgt gcggcggcct
    10441 ctggctggat cgttacgaac tcgcccgctt cgaccggccg gagttcgcgt tcggcgatgc
    10501 gctggtcgcg cacctgcagc agttcccggc gcagctgctc gaacacacgg tccggctgcg
    10561 ctgtccgcat caccgggacg tgacgatgtt gcgacggccg tacgccccgc acgcgcgcgt
    10621 ggaaatcgac gagtgccccc agtgcggcgg agtgtggctc gacgccgacg agctcgcgag
    10681 catccgcgac gcgcgcagcg cccgctcgta ggccgccgcc cagcggaccg gcatcaggtc
    10741 aggtgagcgg cgcgtccgtc ggcgcggcgt acgcaccgcc gcccggcgcg agcagccaga
    10801 cgccgaacca gcgctgcggg tcgaaccacg cctgctgcac gacgagcccg gcctcggcgg
    10861 cgatccgtgc ggccgactcc gccgtgtact tgtgcgagaa ctcggtcagc aggtattcgc
    10921 cgcggcgcag ccgcacgcgg gcggcgccga cgtcgaacgc gacgtcacgg cgcgcgacga
    10981 gccgcatctc gatccgttcg tgcgccgcga cccacggcgc gaagtgccgg aaggcgttcg
    11041 tgtcgatccg cgcgccggtc acccggttga tgtgctcgag cgcattcagg ttgaacgcgg
    11101 ccgtcacgcc cgcggcgtcg tcgtaggctc gctcgaggat ctcgatcggc ttcaccgcgt
    11161 cgaggccgag cagcacgacg ccgccaggcc cggcgatgcg ggcgaaccga cgcagcagcg
    11221 cgaccgcgtc ctgcggggcc cagttgccga gcgtcgaccc ggggaagtac acgacgttgc
    11281 gacgcgcgcc ttcgagcgcc ga
//
LOCUS       DASPFR010000005         2437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_977975, whole genome shotgun sequence.
ACCESSION   DASPFR010000005 DASPFR010000000
VERSION     DASPFR010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977975"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VD737_00115"
     CDS             complement(<1..259)
                     /locus_tag="VD737_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXW08993.1"
                     /translation="MMHDPAESYSMGVEQSAAPLERLLARDGIHEVLARYARGVDRAD
                     AELLKSCYHADAIEEHGGTYTGNAHEYVVGAVVRIRRRGAWQ"
     gene            301..1245
                     /locus_tag="VD737_00120"
     CDS             301..1245
                     /locus_tag="VD737_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXW08994.1"
                     /translation="MVKSSSEVPGRVVIVTGAGTGLGRAYALRLAADGLRVIVNNRRR
                     EVDAAGLGSADRVVEEIRASGGTAIANYDDVCDDGAGERVVRQALDAWGRLDALVNNA
                     GIDQHAAFHRIGLDEFRRIVEVNLMGTVAVTHAAWPHMREAGHGRVVVSSSSAGLHGL
                     HGLSAYAASKAALLAFSRSLAAEGRSRDVLCNAIAPYAATRMTSAHVTDPSEREATAP
                     ELVAPLVSALVSARSTVNGQVFVVGRGWARHAAVVELERLVRVDGASLAADASTQLQR
                     PLLDVSGACREFPDGRASYDDFGARVVAQHLQAGRDAE"
     gene            1336..>2437
                     /locus_tag="VD737_00125"
     CDS             1336..>2437
                     /locus_tag="VD737_00125"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HXW08995.1"
                     /translation="MTLHRILGSACGPRPLACAVASILSAAAVQPVQAQESGGGLEEI
                     VVTAQRRETDLQTTPIAISAYSGQTLAEAKIFSAADLANSVPALSLTAGTPLDVELNI
                     RGITNTRLDSPTADPSVGTFVDGVYIGRTGDYNLDFYDIERIEVIRGPQGVLLGKNVV
                     GGALSVITAAPEFDTSGRVLLGYGNYGAVTASGHVTGGLSDTLAGRASFQIRQHEGYA
                     EDVLHGKKVENLDSTQARAQLLWRGESGWSVRGIVDYTNDSTDGINVVAVAGGTKTCE
                     QEYFRTNCTRPWSNLREYLGLTDPRQNVAQSLQLVGDKRRTQYLDREGWGATVDIEKE
                     FESFTFNSLTGYRTVESNQFYDQTGAGPEALGW"
BASE COUNT          391 a          856 c          815 g          375 t
ORIGIN      
        1 gctgccacgc gcccctccgg cggatgcgga ccaccgcgcc gacaacgtac tcgtgcgcgt
       61 tgcccgtgta ggtgccgccg tgctcctcga tcgcgtcggc gtggtagcag gacttgagca
      121 gttcggcgtc cgcgcggtcg accccacgcg catagcgcgc gagcacctcg tggatcccgt
      181 cgcgcgcgag caggcgctcg agcggcgctg cggactgttc gacacccatg ctgtagctct
      241 ccgccggatc gtgcatcatg cacagaggct tgcttaacaa gactgaaagg aaaaccgccg
      301 atggtcaagt cgtccagtga ggtgccggga cgcgtcgtca tcgtcacggg ggccggcacc
      361 gggctcggcc gcgcctacgc gttgcggctc gccgccgacg ggctgcgcgt gatcgtgaac
      421 aaccgccggc gcgaggtgga cgcggcgggc ctcggcagcg cggatcgcgt cgtcgaggaa
      481 atccgtgcat ccggcggcac cgcgatcgcc aactacgacg acgtgtgcga cgacggcgct
      541 ggcgagcgcg tggtccggca ggcgctcgac gcctgggggc ggctggacgc cctcgtcaac
      601 aacgccggca tcgaccagca cgcggccttc cacaggatcg gcctcgacga gtttcgccgc
      661 atcgtcgagg tgaacttgat gggcacggtc gccgtcacgc atgccgcgtg gccgcacatg
      721 cgcgaagccg gtcacgggcg cgtggtcgtg tcctcgtcgt cggccggcct gcacggcctg
      781 cacggactgt cggcgtacgc ggcgtcgaag gcggcgctgc tcgcgttctc ccgctcgctt
      841 gcggcggaag ggcggtcccg cgacgtcttg tgcaatgcaa tagcgccata cgccgcgacg
      901 cgcatgacct cggcgcacgt cacggacccg agcgagcgcg aggccaccgc gccggaactc
      961 gtcgcgcccc tcgtgtccgc gctggtgagc gcacgctcca ccgtgaacgg gcaggtgttc
     1021 gtggtcggcc gcggctgggc ccggcacgcc gccgtggtcg aactcgagcg actcgtccgc
     1081 gtcgacggcg cgtcgctcgc ggctgatgcg tccacgcaac tgcagcggcc gctcctcgac
     1141 gtgagcggtg cgtgccgtga gttccccgac gggcgcgcct cgtacgacga cttcggcgca
     1201 cgggtcgtcg cgcagcactt gcaggcgggc cgagacgcgg agtagtgtac gccagtctaa
     1261 tttagcaagt gacctgcgtt gccatcgcgc aaccgtgtcc gatcatccgc acgtccgaga
     1321 agggggagct ctctgatgac tctgcatcgc atccttggtt ctgcctgtgg cccgaggccg
     1381 ctggcctgtg ccgtcgcgag catcctgtcg gccgccgccg tccagccggt ccaggcccag
     1441 gaaagcggtg gcggcctcga agaaatcgtc gtcaccgcac agcgtcgcga aaccgacctg
     1501 cagacgacgc cgatcgcgat ctcggcgtac tccggccaga cgctcgcgga ggccaagatc
     1561 ttttcggccg ccgacctcgc caacagcgtg ccggccctgt cgctcaccgc tggaacgccg
     1621 ctcgacgtcg agctcaacat ccgcggcatc accaacacgc gcctcgactc gccgaccgcc
     1681 gacccctcgg tcggcacgtt cgtcgacggc gtctacattg gtcgaaccgg cgactacaac
     1741 ctcgacttct acgacatcga gcgcatcgag gtgatccgtg gcccgcaggg cgtgctgctc
     1801 ggcaagaacg tcgtgggtgg cgcattgagc gtgatcacgg ccgcacccga gttcgacacg
     1861 tccggccggg tcctgctcgg ctatggcaat tacggcgccg tcactgcctc ggggcacgtc
     1921 accggtggcc tcagcgacac cctggcgggt cgcgcctcgt tccagatccg ccagcacgag
     1981 ggctacgccg aggacgttct gcacggcaag aaggtcgaga acctcgattc gacacaggcc
     2041 cgggcacagc tgctctggcg aggcgagagc ggctggtccg tgcgtggcat cgtcgattac
     2101 accaacgact cgaccgacgg catcaacgtc gttgcggtcg cgggcggtac caagacctgc
     2161 gaacaggaat acttccgcac gaactgcacc cgcccgtgga gcaacctgcg cgaatacctt
     2221 ggtctcacgg atccgcgcca gaacgtcgcg cagagcctgc agctcgtcgg cgacaagcgt
     2281 cgtacccagt acctcgaccg ggaaggctgg ggcgccacgg tcgacatcga gaaggaattc
     2341 gaatccttca cgttcaactc gctgacgggc taccgcaccg tcgaatcgaa ccagttctac
     2401 gaccagaccg gagccggtcc cgaggcgctc ggatgga
//
LOCUS       DASPFR010000006         7592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_978893, whole genome shotgun sequence.
ACCESSION   DASPFR010000006 DASPFR010000000
VERSION     DASPFR010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7592
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978893"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="VD737_00130"
     CDS             <1..761
                     /locus_tag="VD737_00130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXW08996.1"
                     /translation="LHRVPTELRSFVTTFNGLLLRLQDAMEGVHRFTADASHQMRTPL
                     SILRAHLQVLQREGLQSAAGQASLADIDAATSRLQRLLTQLLALARAEGSREGLARLA
                     RPVDLVALTRSVAEDYVPVALRAGVDLQFEAAASVVVRTVEEFVRELIANLVDNAVRY
                     NRPGGTVSLRVEPLPDDRSQVTVEDDGPGIPESMRSEVFKRFRRLDRDRGEAGSGLGL
                     AIVKVLADALEADVRLGEGRQGGLRVEIVFSATT"
     gene            769..2421
                     /locus_tag="VD737_00135"
     CDS             769..2421
                     /locus_tag="VD737_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW08997.1"
                     /translation="MPAIVSGVAAVACCAAWLAVPAHAEHSAHARFPAPDCEAYVAYD
                     RDMTLPGYRLRRADGGEVCVPFTTVGPRPPAGYAADFYVAEFAEAKLRNDWLACKTDP
                     ACATRIGKVVAARSPPNREQRIADPHHRYLLGKIAEADPLDLRIVRRPGFFARDPYRE
                     PIAAAESSTYTVEFTAPAEPYERIHRGLDTPVKLRGWYLRGAGVAGLQGRRTRALIVM
                     TGGGGTRLVAIEHPSDRLYRVDPETGRTSINSFPNATTGSSGQAGWRSLIAMFHEAGF
                     DVLAYDRRGVGVSSGYSDTNTLQQGRDLLDALDTLRTGAGLRVLTPSGALLDGVRAAR
                     ELRGRASELPTLLVGNSRGTMSSGWAMTMNFHRDCSYDLPDIACSPPRGNRSIKGALL
                     MAEFTSGPGFVSSTPTPEDEQRGLGRDRGLFIGASQLERNIVFFPGSGILAGMHAWPA
                     AFFARGLWDYAASLEGTINSYDRVAGLKELVVVRGPHPYETWPEVEKQRVRSRMLAFA
                     RAAIHGEKSVPGERTWTTMRELVATTEDAWEVSSAPGGGKGP"
     gene            complement(2418..3431)
                     /locus_tag="VD737_00140"
     CDS             complement(2418..3431)
                     /locus_tag="VD737_00140"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXW08998.1"
                     /translation="MNWRTLGRALALSLVSFVALADAPAALDTTTPRIAINQEASDTA
                     TRLELLQHELLSQQQELMYSTLLRQYDDRMHIRRSLFPSGQALVPAYVFSPRPLDDAR
                     KYPGLVLVHGGFHGRLEWRWFDLIDRAVREGYVVIFPEYRGSSGYGDVHYRNEYGVTD
                     VADVLASADYLVKSSPHVDAERLGIFGHSRGGLVTLLAIEKAPRRFKAAVDVAGLADM
                     VAFMAYKPDFRRLEMASQPHFGGKLPAEDLKPYMEISPLNHVDLIETPLFVAATTGDK
                     VVPLPLHTGRLIDALAARGKTFESKIYEQAPGDHVFLFGDSDERRDLLDRTFAFLRKY
                     LQP"
     gene            3637..3995
                     /gene="ssrA"
                     /locus_tag="VD737_00145"
     tmRNA           3637..3995
                     /gene="ssrA"
                     /locus_tag="VD737_00145"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            4322..4786
                     /locus_tag="VD737_00150"
     CDS             4322..4786
                     /locus_tag="VD737_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW08999.1"
                     /translation="MNKYRIANRLSSEQAAYLAGLIDGEGTIALTRRHAREKRQLVLS
                     ISSTEAELVEWARSATGVGKITRKRTVSEHHAPGLTYSVSNRQALAVIAAVAPYLRSY
                     KRHRAALVLETYITVTRRNGKYDEATLDAKRKFEDAFIAITAGGARRTRARA"
     gene            complement(4858..5235)
                     /locus_tag="VD737_00155"
     CDS             complement(4858..5235)
                     /locus_tag="VD737_00155"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXW09000.1"
                     /translation="MADQSKKDAAIEFLRLASRGDVEQAFDRYVAGGFRHHNPWFAGD
                     AGSLMRAMADNAREFPAKSCEPLRTVVEGDLVCVHSRIRLAPGDRSLAVVHIFRFDGE
                     RIVELWDVGQQEPEEMPNEHGMF"
     gene            complement(5631..>7592)
                     /locus_tag="VD737_00160"
     CDS             complement(5631..>7592)
                     /locus_tag="VD737_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HXW09001.1"
                     /translation="GSDAVAIYAGNPTVHNLGAMLGIGDFIRALRTKNLYSATSVDQL
                     PHMVASWAMFGHQFLMPVPDVDRTQLFVCIGGNPVASAGSLMGAPGFERRVDALRARG
                     GRLLVIDPRRTETAAIADEYLGIRPGTDVFLLLALLHEVFRSGRVQLGHLATLVDGLD
                     TLRDTVLGFDPQALAARTSIPHARVVALAHELLDEPRALVYGRVGACTQEFGGLTLWL
                     IYCLNAVTGHLDTEGGMMFAEPAVDLTRAYGSAGHYGKWRSRVRGLPEFGNELPVAAL
                     AEEVLTAGPGQIRALVTYAGNPVLSTPNGAQLDQALAGLEFFVAIDLYVNETTRHAHL
                     ILPPASPLERSHYDIALSGFAVRNVAKYSPALFAKPADAKHDHEILVELTRRLRKRPP
                     SAGARVSLAIKDVVADRLGPDGALDWMLKTGRYGVPDEGRWNLLSRLPGFGGMRRLLS
                     ARDRRPSGLSLSRLLAEPNGVDLGPLEPALPRRLATRDKRLPLAPAAYLSDLERARHA
                     LMRTAPSLALIGRRHVRSNNSWMHNSHRLVKGRPRCTLLIHPSDAAARNIVDGATVRV
                     SSRVGIVEVAAEVTTDIQPGVVSLPHGWGHGRPGVRLSVASAHAGVSINDLIDDQQVD
                     ALTGTAVLNGTPVEVVAVPVAPPVEASTA"
BASE COUNT         1214 a         2571 c         2607 g         1200 t
ORIGIN      
        1 agctgcaccg ggtgccgacg gagttgcgta gcttcgtgac gacgttcaac ggcctgctgc
       61 tgcgcctgca ggacgccatg gagggcgtcc accgtttcac ggcggacgcc tcgcaccaga
      121 tgcgcacccc gctctcgatc ctgcgcgccc acctgcaggt cctgcagcgt gagggcctgc
      181 agagcgccgc aggacaggcc tccctcgccg acatcgatgc cgccacgagc cggctgcagc
      241 gcctgctgac gcaattgctg gcgctcgcac gcgccgaggg cagtcgcgaa ggactggctc
      301 gactggcgcg gccagtcgac ctcgttgccc tcacgcggtc cgtcgccgaa gactacgtgc
      361 cggtcgcgct gcgagccggc gtcgacctgc agttcgaagc cgccgccagc gtcgtcgtgc
      421 ggacggtcga ggaattcgtc cgcgagttga tcgcgaacct ggtcgacaac gccgttcgtt
      481 acaatcggcc gggtggcacc gtgtcgctgc gcgtcgagcc gctgccggac gaccgctccc
      541 aggtcacggt ggaagacgac ggcccgggga tccccgagtc catgcggtcc gaggtgttca
      601 agcgatttcg ccgcctcgac cgcgatcgtg gcgaggccgg cagcgggctc ggcctcgcga
      661 tcgtcaaggt gctcgcggac gcgctcgagg ccgacgtgcg cctcggtgaa ggccggcaag
      721 ggggactccg tgtcgagatc gtcttctccg ccacgacgtg agtcgcgaat gccggcgatc
      781 gtcagtggag tcgcggccgt cgcgtgctgc gccgcgtggc tggcggtccc cgcgcacgcg
      841 gagcactccg cgcatgcacg gtttccggca ccggactgcg aggcgtacgt cgcctacgat
      901 cgggacatga cgctgcccgg gtaccgcctg cgccgggcgg acggcggcga ggtctgcgtg
      961 ccgttcacca ccgtcggtcc acgacctccc gcgggttacg ccgccgattt ctacgtcgcg
     1021 gagtttgccg aggcgaagct gcgcaacgac tggcttgcgt gcaagaccga tccggcgtgt
     1081 gcgacgcgga tcggcaaggt ggtcgcggcg cgttcgccgc ccaaccgcga gcagcgcatc
     1141 gcggaccctc atcaccgcta cctgctcggc aaaattgccg aagctgatcc tctcgacctg
     1201 cggatcgtcc ggcggccggg gttcttcgcg cgtgatccgt accgcgaacc catcgcggca
     1261 gccgagtcca gcacgtacac cgtcgagttc acggcaccgg ccgaaccgta cgaacggata
     1321 caccgcggcc tcgatacgcc ggtgaaactc aggggctggt acctgcgcgg cgccggggtc
     1381 gccggcctgc aaggccggcg gacgcgtgcg ctcatcgtga tgaccggcgg cggcggcaca
     1441 cgactggtcg cgatcgagca cccgtcggac cggctctatc gcgtcgatcc ggagaccggc
     1501 aggacgtcca tcaattcctt cccgaacgcc accaccggat cgtcgggcca ggccggctgg
     1561 cgctcgctga tcgcgatgtt ccacgaggcg ggcttcgacg tgctcgccta tgaccgtcgc
     1621 ggcgtgggcg tctccagcgg ctacagcgac accaacacgc tgcagcaggg gcgcgatctc
     1681 ctcgacgcgc tcgacaccct gcgcaccggc gcggggctgc gcgtgctgac gccgtcggga
     1741 gcgctgctcg atggcgtgcg ggcggcgcgc gagctgcgtg gacgggcctc cgagctgccc
     1801 accctgctcg tcggcaactc ccgcgggacc atgtcgagcg gctgggcgat gacgatgaac
     1861 ttccaccggg actgcagtta cgacctgccg gacatcgcct gctcgccgcc gcgcggcaac
     1921 cgctcgatca agggcgcgtt gctgatggcg gaattcacga gcggcccggg tttcgtctca
     1981 tcgacgccga cgccggagga cgaacagcgt ggcctcggcc gcgaccgcgg cctgttcatc
     2041 ggcgccagcc agctcgaacg taacatcgtg ttcttcccgg gctcgggcat cctcgcgggc
     2101 atgcacgcgt ggcctgcggc gttcttcgcg cgcgggctgt gggattacgc cgcgagcctc
     2161 gaaggcacga tcaattccta cgatcgagtc gccgggttga aggaactcgt cgtcgtgcgc
     2221 ggaccgcatc cctacgagac ctggcccgag gtcgagaagc agcgcgtgcg cagccgcatg
     2281 ctcgcattcg cgcgtgctgc gatccacggc gagaagtccg tgcctggcga acggacgtgg
     2341 accacgatga gggaactcgt cgccacgacc gaggacgcct gggaggtgtc ctcggcgccg
     2401 ggcggcggca aaggccccta gggctgcagg tatttccgga ggaacgcgaa cgtccggtcg
     2461 aggaggtccc gtcgctcgtc gctgtccccg aacaggaaca cgtggtcgcc gggcgcctgt
     2521 tcgtagatct tcgactcgaa cgtcttgccg cgcgccgcga gtgcgtcgat caggcgaccg
     2581 gtatgcagcg gcagcggcac gaccttgtca cccgtcgtgg cggcgacgaa cagcggggtc
     2641 tcgatgaggt ccacgtggtt cagtggcgag atttccatgt agggcttgag gtcttccgcg
     2701 ggcagcttgc cgccgaaatg aggctgactc gccatctcga gccgacggaa gtccggcttg
     2761 tacgccatga aggcgaccat gtcggcgagc cccgccacgt ccaccgccgc cttgaacctg
     2821 cgtggcgctt tttcgatggc caacagcgtc acgagcccgc cgcggctgtg accgaagatg
     2881 ccgagccgct ccgcgtccac gtggggagac gatttgacga ggtagtcggc ggaggccagc
     2941 acgtcggcga cgtcggtgac gccgtactcg ttgcgatagt gcacgtcgcc ataccccgaa
     3001 ctgccccggt attccgggaa gatcacgaca tagccctcac gcacggcgcg gtcgatcagg
     3061 tcgaaccagc gccactccag ccggccgtga aacccgccgt ggacgagcac cagcccgggg
     3121 tacttgcggg cgtcgtcgag cggcctcgga ctgaacacgt aggccggcac gagcgcctgt
     3181 ccgctcggaa acaggctgcg gcgtatgtgc atgcgatcgt cgtactggcg cagcagcgtc
     3241 gagtacatca gctcctgctg ctgcgacagc agttcgtgct gcagcagttc gagccgggtg
     3301 gccgtgtcgc tcgcttcctg gttgatcgcg atgcggggcg tggtggtgtc gagtgctgcc
     3361 ggtgcgtcgg caagcgccac gaacgaaacc aaagagaggg cgagtgcacg accgagagtt
     3421 cgccagttca ccaacggacc tccccgggcg ccgccggagc ccacgatgga gcgaaacgta
     3481 cgtgcggttc ctgtcgcagg gctgtcgcgc agggcggatc ccggcggtgg cggacccggg
     3541 cacgacgtaa aggggcgaac aatctcgcaa atacgacccg gcaggcgcga tccgcttgta
     3601 ttccggcacg atgcccctac acttggcgtc gttcctgggg gcgaccggtt tcgacgtggg
     3661 tcgcgaaact tcaggtgcat gccgaggtgc aaggtacctc gttaaaccac ttgcaaactt
     3721 atagttgcca acgacgacaa ctacgctctg gcggcttaat cccgcctaac ccccgactgg
     3781 catgtgcctg tgcatccaga cccgggggac gcgctcacag gctcgcgtgt ccagtgtgct
     3841 gagggctggc tcgctaaaac gttcctgagc tggtcggtca tcttccctgc cgcttgggta
     3901 gtgcccgacg agaatcaaaa agccggctaa gcatgtaggt cctggaacgt agggcttgcg
     3961 gacgcgggtt cgatcaggaa gggtcgcctt cacccgtaag ggtgaagtga tcacggggct
     4021 cacatcggtg aagcctgacg tgccgaggat ggcggcacga ggtaacgccg aggggtagca
     4081 agagggccaa cctcagggct gccctgtaga gactcataga ccggctgagc tgcatgcagc
     4141 ggagccgtta ctaggattcg ggcggttacg tccggataac acgccccgcc cccggctccg
     4201 gtcggggtga aggcatagtc cagcctgtct cggaagaggc agattcgctg tcccgccgcc
     4261 tccaccatca ctgacccctc ggccgcgagg ccgaggggtc tttttcgtgg caggcagtgt
     4321 cgtgaacaaa tacaggatcg ccaaccggtt gagcagcgag caagcggcgt atctggctgg
     4381 tcttatcgac ggcgaaggca cgatcgcgct caccaggcgg cacgctcgag agaagcgtca
     4441 gctcgtcttg tcgatcagca gcactgaagc cgaactcgtc gaatgggcaa gatccgcgac
     4501 gggcgtcggt aagatcaccc gcaaacgaac cgtttccgaa caccacgctc cgggcctgac
     4561 gtattcggtg tcgaatagac aggctctcgc ggtgatcgcg gccgtggcgc cgtacctgcg
     4621 ctcatacaag cgacacaggg ctgcgctcgt actcgagact tacatcactg tgacccgccg
     4681 taacgggaag tacgacgagg cgaccctgga cgccaaacgc aagttcgagg acgcgttcat
     4741 tgcgatcacg gccggcgggg cacgaagaac gcgagcgcgt gcctgatcgt atgtccaaca
     4801 ccgttcacgg gtagacgatg cgatgggact gtcaggccag ctgctgatcc tttcgatcta
     4861 gaacatgccg tgttcgttcg gcatctcctc cggttcctgc tggccgacgt cccagagctc
     4921 gacgatccgc tcgccatcga agcggaagat gtgcacgacg gcgagcgagc gatcgcctgg
     4981 cgcgagccgg atccgcgaat gaacgcagac caggtcgccc tcgacgaccg tgcgcagagg
     5041 ctcgcaagac ttggccggaa attctcgcgc gttgtccgcc attgcgcgca tcagtgatcc
     5101 agcgtcgccc gcgaaccacg ggttgtgatg acgaaacccg ccggccacgt accggtcgaa
     5161 tgcctgctcg acgtcccctc gcgacgcgag ccgcaagaac tcgatggccg cgtccttctt
     5221 gctttgatct gccatcgtcc gcacctcgtt cgcggctcac ctgccagatg aaagaactcc
     5281 ccgcggccgc cgcgcgtccg ggggagttcc ggtggtgcgc gtcatacagg cggcgcgcac
     5341 ggcccgcctc gactcagcgc cgctggttac cgccactgcc gccgcggtcg tcgtccttgc
     5401 gatccggcct gctgccctcc ctggctcgtc cctgagccac ctgatcagac agtcgggccg
     5461 tccctggccc tccgtctcgc gtttccatgc gagcggcgaa catagcaacg cggttggcgc
     5521 ggcacatcgg cgtcgcgccg cagtggtcgt aggatttgac gctctcgccg gcggcacggc
     5581 gtccgccgcg ggtgatgtgc tcgctgcgcc gggcgagcgc cgcgcgccga tcaggccgtg
     5641 gaagcctcga cgggcggtgc gaccggcacc gccacgacct cgaccggtgt gccgttcaac
     5701 acggccgtac cggtcagtgc gtccacctgc tgatcgtcga tgaggtcgtt gatgctgacg
     5761 cctgcgtgcg cgctcgcgac ggacaagcgt accccggggc gaccgtgccc ccagccgtgc
     5821 ggcaggctga cgacgccggg ctggatgtcg gtcgtcactt ccgccgcgac ctcgacgatg
     5881 cccacgcgcg aacttacgcg caccgtcgcg ccgtcgacga tgttgcgcgc ggcggcgtcg
     5941 ctcggatgga tcagcaacgt acaacgcggc ctgcccttca ccaggcggtg gctgttgtgc
     6001 atccacgagt tgttgctgcg cacgtgacga cgcccgatca gcgcgagcga cggcgccgtg
     6061 cgcatgagcg cgtgacgggc ccgttcgagg tcagacaggt atgccgctgg cgcgagtggc
     6121 aggcgcttgt cccgcgtcgc caggcggcgc ggcagtgccg gctcgagcgg acccaggtcg
     6181 acgccgttcg gctccgcgag cagccgcgac aacgacagcc ccgacgggcg gcggtcgcgt
     6241 gccgacagca gcctgcgcat gccgccgaag cccggcagcc gtgacagcag gttccagcgg
     6301 ccctcgtccg gcacgccgta gcgtccggtc ttcagcatcc agtcgagcgc gccgtcgggc
     6361 ccgagccggt cggcgacgac gtccttgatt gccagcgaga cgcgtgctcc cgcggacggc
     6421 ggccgcttgc gcaggcgccg cgtcagctcc accaggatct cgtgatcgtg cttcgcgtcc
     6481 gcgggtttcg cgaacagcgc gggcgagtac ttcgcgacgt tgcgcacggc gaagccgctc
     6541 agcgcgatgt cgtagtggct gcgttcgagc gggctcgcgg gcggcaggat caggtgcgcg
     6601 tgtcgcgtcg tctcgttgac gtagaggtcg atggcgacga agaactcgag tccggcgagc
     6661 gcctggtcga gctgcgcgcc gttcggtgtc gagagcacgg gattgcccgc gtacgtcacc
     6721 agcgcgcgga tctgccccgg accggcggtc aggacctctt ccgccagcgc cgcgaccggc
     6781 agttcgttgc cgaactcggg cagcccgcgc acgcgcgagc gccacttgcc gtaatggccg
     6841 gccgagccat aggcgcgcgt gaggtcgacg gccggctcgg cgaacatcat gccgccctcg
     6901 gtgtcgaggt gaccggtgac ggcattgagg cagtagatca gccacagggt caggccgccg
     6961 aactcctgcg tgcaggcacc gacgcgtccg tagacgagcg cgcgtggctc gtcgagcagc
     7021 tcgtgcgcca gggcgacgac ccgcgcgtgc gggatcgacg tgcgcgccgc gagcgcctgc
     7081 ggatcgaacc ccagcaccgt gtcccgcaac gtgtcgagcc cgtccaccag cgtcgccagg
     7141 tggccgagct gcacgcgacc gctgcgaaac acttcgtgca gcagcgccag cagcaggaac
     7201 acgtccgtgc cgggccggat gcccaggtat tcgtcggcga tcgcggccgt ctcggtgcgg
     7261 cgcgggtcga tcacgagcaa gcgtccgcca cgggcgcgca acgcgtcgac ccgtcgctcg
     7321 aagccggggg cgcccatcag cgagcccgcc gaggccaccg ggttgccgcc gatgcagacg
     7381 aacagctgcg tgcggtcgac gtccggcacc ggcatcagga actggtgccc gaacatcgcc
     7441 cacgacgcga ccatgtgcgg cagctggtcg acgctggtgg ccgaatagag gttcttcgtg
     7501 cggagcgcgc ggatgaagtc gccgatgccg agcatcgcgc cgaggttgtg cacggtcggg
     7561 ttgccggcgt agatcgcgac ggcatcgctg cc
//
LOCUS       DASPFR010000007         3260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1223516, whole genome shotgun sequence.
ACCESSION   DASPFR010000007 DASPFR010000000
VERSION     DASPFR010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223516"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VD737_00165"
     CDS             complement(<1..414)
                     /locus_tag="VD737_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09002.1"
                     /translation="MRSAIASSLLALVGSIVLPGSHAETRPESSQASVDYVADGRAPL
                     DELARRFTELDAKHGWAVETVYAYPDAPDLQIRAWRTAATGPALWVLAGIHGEEPAGP
                     NAIARELDALVDFARSGVPVVVVPLCNPRAYRHNWR"
     gene            complement(414..1811)
                     /locus_tag="VD737_00170"
     CDS             complement(414..1811)
                     /locus_tag="VD737_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXW09003.1"
                     /translation="MRPDPDELEPTRRRVTGEDPEPPAQRKELGFWMCTALVVGNTIG
                     IGIYVLPASLAPYGFNAMLGWAITVVGMTVLARVFARLAREFPSADGPHAYIERTTGT
                     LAAFVAIWCYWVSCWITNAAIAIGVVGYLNKVLPGLASVTPALQALAMVWLFVVVNLL
                     GVRAGGRVQVLTTVLKLLPMAYIVGLGAWVLVTEPATYARHPPSTPISLEALMAASTI
                     ALFAMLGIESAAVPAGRVRDPARTIPRSTMAGTLLTAAIYIAVSSMALLLLEQERLAQ
                     SAAPFADLLDDYGGSSVGRLLAVFVVVSGLGALNGWTLLVGELTASLGRHGFLPTAAR
                     RTNARGAPAVGLVVTGLLATAMVLMNYSKSLVEGFTFLTLIVTAANLPLYLLCALALI
                     VLARRGERRLPASMLVLGVIGTAYSVFAFVGLGHEPFLWSLVLGAAGLPVYWALRHRR
                     AVGVTSVAPADGRDA"
     gene            complement(1801..3120)
                     /locus_tag="VD737_00175"
     CDS             complement(1801..3120)
                     /locus_tag="VD737_00175"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HXW09004.1"
                     /translation="MPNPHIKTPLPGPKARAMLERDKPVASPSYPRDYPFVMSHGRGT
                     EVWDVDGNRFLDFMSGIAVCSTGHSHPKVVEAVKAAADRFLHISSDFWHENFTALCER
                     LARHAPMGEPVMSFLGNSGTEAVEGAIKLARAVTGRPRYLGFLGSFHGRTMGSLSFTS
                     SKYTQQKGFAPTMPGVTHVPYPNVYRPLLAGNDQGKAVLDYIRMLFERNVPPGEVAAI
                     LVEPIQGEGGYLVPPDGFLAGLRALCDEHGILLIFDEVQSGVGRTGKMWASQHWGVAP
                     DIMTSAKGLGSGMPIGAFIAKASIMSKWQRGAHGNTYGGNPLSCAAAIATLDLVESGY
                     AANAARVGEYFMTRLRSLQTQYPCIGEVRGRGLMIGMELIEEGDRAPARQLIDRLVTR
                     GFHNGLLLLSCGVSTLRFMPPLCVTSDQIDEAVEMLRTSLDEALGAA"
BASE COUNT          538 a         1116 c         1139 g          467 t
ORIGIN      
        1 gcgccagttg tggcggtacg ccctgggatt gcagagcggc acgaccacga ccggaacgcc
       61 ggaccgcgcg aagtcgacca gcgcgtcgag ttcccgcgcg atcgcgttcg gccccgcggg
      121 ttcttcgccg tggatgccgg ccagcaccca gagtgcaggc cccgtcgcgg ccgtgcgcca
      181 ggccctgatc tgcaggtccg gcgcgtcggg ataagcatag accgtctcga cggcccagcc
      241 gtgcttcgcg tcgagctcgg tgaaacggcg cgccagttca tcgagcgggg cgcggccgtc
      301 ggcgacgtag tcgaccgagg cctggctcga ttccggtcgc gtctccgcgt gtgagcccgg
      361 caggacgatc gaaccgacca aggccagcag gcttgaggcg atcgcagagc gcatcaggca
      421 tccctcccat ccgccggggc aaccgacgtc acgccgaccg cgcgtcggtg ccgcaacgcc
      481 cagtacaccg gcaggccggc cgcgcccagc accagcgacc acaggaacgg ctcgtgcccg
      541 aggccgacga atgcgaagac cgagtacgcc gtgccgatca cgccgagcac gagcatgctc
      601 gcgggcaagc gacgttcccc gcgacgtgcg agcacgatga gcgccaacgc gcagagcagg
      661 tagagcggca ggttcgcggc cgtgacgatc agcgtcagga acgtgaaccc ctcgaccagc
      721 gacttgctgt agttcatcag caccatcgcc gtggcgagca ggcctgtgac gaccagtccg
      781 acggccggcg cgccgcgcgc gttggtccgt cgtgccgccg tcggcaggaa gccgtgacga
      841 ccgagcgagg ccgtgagttc accgaccagc agcgtccagc cgttgagcgc acccagcccg
      901 ctcacgacca cgaacacggc gagcaaccgg cccacggagc tgccgccata gtcgtcgagg
      961 aggtccgcaa agggcgcggc cgattgcgcc agccgttcct gctcgagcag cagcagcgcc
     1021 atcgaggaca ccgcgatgta gatggccgcg gtcagcagcg tgccggccat cgtcgaacgg
     1081 ggaatggtcc gggccggatc gcgcacgcga ccggccggca ccgccgccga ttcgatgccg
     1141 agcatcgcga acagcgcgat cgtcgaggcc gccatcaggg cttcgagcga gatcggcgtc
     1201 gacggcgggt gccgcgcgta ggtcgcgggc tcggtcacca ggacccacgc gccgaggccg
     1261 acgatgtagg ccatcggcag cagcttgagc acggtcgtga gcacttgcac gcgaccaccg
     1321 gcgcgcaccc ccagcaggtt caccacgacg aacagccaca ccatcgcgag cgcctgcagt
     1381 gcgggcgtga cgctggccag ccccggcagc accttgttca ggtagccgac gacgccgatc
     1441 gcgatcgcgg cattggtgat ccagcaggag acccagtagc accagatcgc gacgaaggcg
     1501 gcgagcgtgc cggtggtgcg ttcgatgtag gcgtgcggac cgtccgcgct cgggaactcg
     1561 cgcgccagcc gcgcgaacac gcgagcgagc accgtcatcc cgacgaccgt gatcgcccag
     1621 ccgagcatcg cgttgaagcc atacggcgcg agcgaagccg gcaacacgta gatgccgatg
     1681 ccgatcgtgt tgccgaccac gagggccgtg cacatccaga agccgagttc cttgcgctgc
     1741 gccggcggtt ccgggtcttc tccggtgacg cgacgtcgtg tcggctccag ttcgtcgggg
     1801 tcaggccgca cccagggcct cgtcgagcga ggtgcgcaac atttccacgg cctcgtcgat
     1861 ctggtccgac gtgacgcaga gcggcggcat gaagcgcagg gtgctcactc cgcacgagag
     1921 cagcagcagg ccattgtgga agccgcgcgt gaccagacgg tcgatgagct gccgtgccgg
     1981 cgccctgtcg ccttcctcga tcaactccat gccgatcatg agcccacggc cgcgcacttc
     2041 gccgatgcac gggtattgcg tctgcagcga gcgcaggcgc gtcatgaagt actcaccgac
     2101 cctggccgca ttggcggcgt acccggattc gacgaggtcg agcgtcgcga tcgcggccgc
     2161 gcagctcagc ggattgccgc cgtaggtgtt gccgtgcgcg ccgcgctgcc atttcgacat
     2221 gatcgaggcc ttggcgatga aagccccgat cggcatgccg gagccgaggc ccttggcgct
     2281 ggtcatgatg tcgggcgcga cgccccagtg ctgcgacgcc cacatcttgc ccgtccggcc
     2341 gacgccggac tgcacttcgt cgaagatcag caggatgccg tgctcgtcgc agagcgcgcg
     2401 caggcccgcg aggaacccgt ccggaggcac caggtaaccg ccttcaccct ggatcggctc
     2461 gacgaggatg gcggcgacct cgccgggcgg gacgttgcgc tcgaacagca tgcggatgta
     2521 gtcgagcacc gccttgccct ggtcgttgcc ggcgagcagc gggcggtaga cgttggggta
     2581 gggcacgtgc gtcaccccgg gcatggtcgg cgcgaagccc ttctgctgcg tgtacttgct
     2641 ggaggtgaac gagagcgagc ccatcgtgcg gccgtggaag ctgccgagga acccgaggta
     2701 acgcggccgg cccgtgacgg cgcgtgcaag cttgatcgcg ccttcgacgg cctcggttcc
     2761 cgagttgccc aggaagctca tcaccggctc ccccatcggg gcgtgccgcg cgagacgctc
     2821 gcacagcgcc gtgaagttct cgtgccagaa gtcgctcgag atgtgcagga aacggtcggc
     2881 cgccgccttg accgcttcga cgacctttgg gtggctgtga ccggtcgagc acacggcgat
     2941 gccggacatg aagtcgagga acctgtttcc atccacgtcc cagacttccg tcccgcgtcc
     3001 gtgcgacatg acgaagggat agtctcgcgg gtacgacggc gaggcgacgg gcttgtcgcg
     3061 ctcgagcatc gcgcgcgcct tggggccggg caacggcgtc ttgatatgcg ggttgggcac
     3121 gggtgggctc ctggtttttt cctgcaggct catgcgggca ggcgcacggg tcgccgatgc
     3181 gcctcgcgcg tgcgcgcgtg cagtgcgcgc agcgtatcca attcgagggg actcggtggc
     3241 tcgagcgccg cgacctctgt
//
LOCUS       DASPFR010000008         3151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1345457, whole genome shotgun sequence.
ACCESSION   DASPFR010000008 DASPFR010000000
VERSION     DASPFR010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345457"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="VD737_00180"
     CDS             <1..1135
                     /locus_tag="VD737_00180"
                     /inference="COORDINATES: protein motif:HMM:NF016174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09005.1"
                     /translation="AISGVHFNYSFPERFWPVLAETFQARQADRDFRSDVYFALLRNY
                     RRHGWIVLYLFGNSPAICPSFLQGRKIDYLEPIEPGSLYAPYATSLRMSDLGYRNSAQ
                     ADLSISVNSLEHYVRDLSAAITTPHPDYERIGVKVDGEYRQLNANVLQIENEYYSFIR
                     PKRVTQTGERPTRALQRGGVQYVEMRSLDCSAFDPVGVNQNKLRFLEAFAAFCVLRES
                     RPIETSEQGDLDGNHAIVAREGRRPGLQLRRDGRAQPMAVWAAEIIDSMRGVCELLDE
                     GDPKRPYTTALELQEEKVRDVTRTPSARTLHELRTNEESFFNFALRMSKVHQAYFRDL
                     FPPNPARQHEFAAEAIESIEQQKRIEATDSLSFDEYLHQYFAR"
     gene            1345..1620
                     /locus_tag="VD737_00185"
     CDS             1345..1620
                     /locus_tag="VD737_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09006.1"
                     /translation="MTSSTTTGRRSPLQHWLEFLASARSDAHRSTHVEARTYVEVVQA
                     QREDVTVGWDPHEVWLDRIKRPRDRRIAASLEPSTDPAPEATVKESR"
     gene            complement(1604..2536)
                     /locus_tag="VD737_00190"
     CDS             complement(1604..2536)
                     /locus_tag="VD737_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HXW09007.1"
                     /translation="MQAIAVHGGAGDIPPHELTAERERDYHAGLARALEAGRKILARR
                     GSSLDAVVAAVQALEEDTLFNAGRGSVIAANGLCELDASLMEGRQLRAGAVTGVRHVR
                     SPIHLARLVMERSPHVMLSGEGAEEFALEQGLAPVANSHFVTERRQRELERALHDHVS
                     ASTSPQVNAAAGGVPKGTVGAVALDEHGDLSAATSTGGMTAKRWGRVGDSPIIGAGTY
                     AANDCCAVSATGHGEFFIRAAVAHEIASLMRYAGLDVAGAAERVVHEQLVRLGGEGGV
                     IAIGRDGRIAMPYNSKGMLRGSIDAAGQVFTAIL"
     gene            complement(2642..3106)
                     /gene="trxC"
                     /locus_tag="VD737_00195"
     CDS             complement(2642..3106)
                     /gene="trxC"
                     /locus_tag="VD737_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004265454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HXW09008.1"
                     /translation="MATPATDAQSAQLVCSHCESLVRVPSGRLHDAPKCPRCHAALFE
                     GRPITLTDDNFDRHVTRTTVPIVVDFWAPWCGPCRSMAPVFEQVAAAIEPAARFAKLD
                     TDAAPQAAARYGIRSIPTLIVFRDGREIARQAGAMDPASLRRWIAQGIGDRG"
BASE COUNT          529 a         1084 c         1050 g          488 t
ORIGIN      
        1 ggcgatttca ggggtgcact tcaactattc gttcccggag cgcttctggc cggtgctggc
       61 cgaaacgttc caggccaggc aggccgatcg ggacttccga tccgacgtct acttcgcgct
      121 gctgcgcaac taccgtcggc acggctggat cgtgctttac ctgttcggca actcgccggc
      181 gatctgcccg tcgttcctgc agggccgcaa gatcgactac ctggagccga tcgagccggg
      241 aagcctgtac gccccgtacg ccacctcgct ccgcatgagc gacctggggt accgcaacag
      301 cgcccaggcg gacctgagca tctccgtcaa cagcctcgag cactacgtcc gcgacctgtc
      361 ggcggccatc acgaccccgc accccgacta cgagcgcatc ggcgtcaagg tcgacggcga
      421 gtaccggcag ctcaacgcca acgtgctgca gatcgagaac gagtactaca gcttcatccg
      481 tccgaagcgg gtcacgcaga ccggcgagcg tccgacgcgc gcactgcagc gaggcggagt
      541 gcagtacgtc gagatgcggt cgctcgactg cagcgcgttc gacccggtcg gcgtgaacca
      601 gaacaagctg cggttcctcg aggcgtttgc ggcgttctgc gtgctgcggg agagccggcc
      661 catcgagacg tccgagcagg gcgatctcga cggcaaccac gcgatcgtcg cgcgggaggg
      721 ccgcaggccg ggtctgcagc tccggcgaga cgggcgcgct cagccgatgg ccgtgtgggc
      781 cgccgagatc atcgattcga tgcgcggcgt ctgtgaactg ctcgacgagg gcgacccgaa
      841 acgtccgtac accacggcgc tcgagctgca ggaggaaaaa gtccgcgacg taacgcgcac
      901 gccgtcggca cgcacgctcc acgagttgcg caccaacgag gagtcgtttt tcaacttcgc
      961 gctgcgcatg tcgaaagtgc accaggcgta tttcagggac ctctttccac cgaacccggc
     1021 gcggcagcat gagttcgctg ccgaagcgat cgaatcgatc gagcaacaga agcgcatcga
     1081 ggcgaccgac tcgctctcgt tcgacgaata cctgcaccag tatttcgcgc gttgacccgc
     1141 cggccgttcg acggcgctcc gcacatcggc acgaacgcgc ggtgtgtgtc gttcacggcg
     1201 cgcgtccaac tagtccttat tgtccgggcg acatcgtttt gtcacaacga cgaggcactc
     1261 tgctgccagg tgtcaaggat atcgacggcg gtgggtgcga gcggctcgca tcgcataccg
     1321 cgagccgggg agagcgatcg cgcgatgacg agcagcacga cgacgggccg acgttcgccc
     1381 ttgcagcact ggctggaatt cctggcttct gcacgctccg atgcgcatcg atcgacgcac
     1441 gtcgaggcgc gcacttacgt ggaggtcgtg caggcgcagc gcgaggacgt gaccgtgggg
     1501 tgggatccgc acgaagtgtg gctcgaccgg atcaagaggc cgcgggaccg gcgtatcgcc
     1561 gcgtcgctcg agccgtcgac cgatcccgcg ccggaagcga cggtcaaaga atcgcggtga
     1621 aaacctgccc cgcggcatcg atcgagccgc gcagcatccc tttcgagttg tacggcatcg
     1681 cgatgcgccc atcgcgaccg atggcgatca cgccgccttc gccgcccagg cgcaccagct
     1741 gctcgtgcac gacgcgctcc gccgcgccag cgacgtcgag cccggcgtaa cgcatcaacg
     1801 acgcgatctc gtgcgccacc gccgcgcgga tgaagaattc gccgtgaccg gtcgcggaca
     1861 ccgcgcagca atcgttggcg gcgtacgtac ccgcgccgat gatcggcgaa tcgccgacgc
     1921 ggccccaccg tttggcggtc atgcccccgg tcgaggtcgc agccgaaagg tcgccgtgct
     1981 cgtcgagcgc gacggctccg accgtccctt tcggaacgcc gcccgctgcg gcgttcacct
     2041 gcggtgaggt ggacgcgctg acgtggtcgt gcagcgcgcg ctccagttct cgctgccgac
     2101 gctcggtcac gaagtgactg ttggccacgg gcgcgagccc ctgttcgagc gcgaactcct
     2161 cggccccttc gcccgacagc atcacgtgcg gcgatctctc catcaccagc cgcgcgaggt
     2221 ggatggggct gcgcacgtga cgcacgccgg tcaccgcgcc ggcacgcaac tggcgaccct
     2281 ccatcagcga cgcgtcgagc tcgcagagcc cgttcgccgc gatcaccgag ccgcgaccgg
     2341 cgttgaacag cgtgtcctct tcgagcgcct ggaccgccgc gacgacggcg tcgaggctcg
     2401 agccgcgacg cgccaggatc ttgcgcccgg cttccagcgc gcgcgcaagc ccggcgtgat
     2461 agtcacgctc gcgctcggcg gtcaactcgt gcggcggaat gtcacctgcg ccgccgtgta
     2521 cggcaattgc ctgcatctac gaacgcccgg tgcgtgctac ggaggagtcg caccataccg
     2581 aaagcgcggc gcaaaccgaa acgccgacga aggggctgat cggaaatctc ggtccgtttt
     2641 gctatcccct atccccgatc ccctgggcga tccagcggcg caggctggca ggatccatgg
     2701 cgcccgcctg gcgagcgatc tcacggccgt ccctgaacac gatcagcgtc gggatgctgc
     2761 ggataccgta acgcgcggcc gcctgcgggg cagcgtccgt gtcgagcttg gcgaacctgg
     2821 cggcgggctc gatggctgcg gccacctgct cgaacaccgg cgccatcgat cggcagggcc
     2881 cgcaccaggg cgcccagaag tcgaccacga tcggcacggt ggtccgggtc acgtggcgat
     2941 cgaagttgtc gtcggtcagc gtgatcgggc gtccctcgaa caaggcggcg tgacatcgcg
     3001 ggcacttggg ggcgtcgtgc agcctgccgg acggcacgcg gaccaggctc tcgcagtgcg
     3061 agcaaaccag ctgggcggac tgggcatcgg tggcaggggt ggccatggtg cgcggctcct
     3121 tcgtcgtcaa aaccgtccgt gcgcttcatg g
//
LOCUS       DASPFR010000009         6805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_735833, whole genome shotgun sequence.
ACCESSION   DASPFR010000009 DASPFR010000000
VERSION     DASPFR010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6805
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735833"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VD737_00200"
     CDS             <1..510
                     /locus_tag="VD737_00200"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HXW09009.1"
                     /translation="HRDNTIGATPQPNPGDDDWVRFFREHRLAHMLDLAAARGLPSRT
                     VDRGRELIERCPALFAGYRPVPSLLHGDLWGGNWSAHALTCEPVVFDPAVYRGDREAD
                     LAMTHLFGGFGPDFYAAYGAQWPLEPGAGARRPLYNLYHVLNHFVLFGGGYARQADAM
                     IGRLLADLG"
     gene            complement(523..1299)
                     /locus_tag="VD737_00205"
     CDS             complement(523..1299)
                     /locus_tag="VD737_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09010.1"
                     /translation="MRTDPFRPVRLLVPGLALLALAAPAAAWDMCEHRAERRASLDTA
                     GVARVVINARAGDLEVKPTDGTTLVAAGPACASSAKFLEQTDIAVRREGDTLRVDVLV
                     PDRMVGIGVFYATLDLTVDVPAALPIEITDSSGDVLAADVRIVKLTDSSGDVLLRHPR
                     ADIEIWDSSGDVRIEDAAGQVQVRDSSGDIVIVGAKEVVIPSDRSGDISIRRVAGSVR
                     IDQDSSGDIRVADVGRDFQLLSDSSGEVKVSGVKGKVQLP"
     gene            complement(1427..2851)
                     /locus_tag="VD737_00210"
     CDS             complement(1427..2851)
                     /locus_tag="VD737_00210"
                     /inference="COORDINATES: protein motif:HMM:NF010000.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HXW09011.1"
                     /translation="MQTKLLINGNLVKGEGQPEDILDPATGKSLAKVAAASRGQIDAA
                     VKAADAAFDGWARTPPKDRAAVLLKLADRIEADGAAFAKLESQNCGKPFAAALNDEIP
                     AIADVFRFFAGACRTMTGAVAGEYLAGYTSMIRRDPIGVVASIAPWNYPLMMAAWKLG
                     PALAAGNTAVLKPSEQTPLTALKLAELLAEIFPPGVVNVVCGRGSSVGAPLTRHRLVR
                     MISLTGDVATGEKILGQAADGIRRTHLELGGKAPVIVFDDADVESVVSGVRTFGYYNA
                     GQDCTAACRIYAGAKVYDRLVADLSSAVRSIKVGAQTSKDVEMGPLISAGQRDRVAGF
                     VARAKKQKHIEVTAGGHAGGGGGFFFEPTVIANARQSDEIVQREVFGPVVSVTRFKDA
                     DEAVRWANDSDYGLASSVWTRDVGRALEVASRLQYGCTWINTHFMLVSEMPHGGLKRS
                     GYGKDLSAYALEDYTVVRHVMAKF"
     gene            complement(2980..3798)
                     /locus_tag="VD737_00215"
     CDS             complement(2980..3798)
                     /locus_tag="VD737_00215"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXW09012.1"
                     /translation="MRSESRRKPRWQRASRLTVAFAALGLLFLHVPVWVIALYAFTTE
                     DRTYQFPPPGLTLHWFEVAFARSDIWAAMRLSLLVATVATLVALVLGTLAAFALARRT
                     FRGKESITLLFVLPIALPGILTGLALLAGIRTLGVEPGFWTIVAGHATFCLVIVYNNV
                     IARLRRIPVSWVEASGDLGARGHQTFRYVLLPQIASALLAGGMLAFALSFDEIIVTLF
                     TSGHERTLPIWFYNELFRPRERPVTNVVAVIVILVTLLPILGAYWLSRPEKESG"
     gene            complement(3788..4696)
                     /locus_tag="VD737_00220"
     CDS             complement(3788..4696)
                     /locus_tag="VD737_00220"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXW09013.1"
                     /translation="MARLPAMSLGGRRAFYFALLLAPPLLWLGIVYLGSLFTLLMQSF
                     YAIDEFTARVVPEPTLATYEVLLTRPAHLDIILRTLAMAVAVTIASAVIAFPVALYMA
                     RHAGPRLKALFYVGILLPMWTSYLVKVYAWRLILAKQGIASWSLEQVGLAGALDRVLA
                     VPVVGGPSLASSYLGMFVVFVYMWLPFMILPLAAALERVPDSLLQASADLGATPRQTF
                     RHVVLPLALPGLAAGSIFTFSLTLGDYIIPSVVGAPGYFIGMMVYQQQGTAGNLPLAA
                     AFSVVPIVLIGAYLALARRLGAFDAL"
     gene            complement(4699..5712)
                     /locus_tag="VD737_00225"
     CDS             complement(4699..5712)
                     /locus_tag="VD737_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXW09014.1"
                     /translation="MTAAIELRDVGRTFGAVVALDRVSFAIAPGEFFALLGPSGSGKT
                     TCLRLIAGFDQPDAGRVLLGGEDVTDTPPYARDVNTVFQDYALFPHMSVADNVAYGLR
                     IRGIDASERRSRAREMLELVRLGELGARRPYELSGGQRQRVALARALINRPKVLLLDE
                     PLGALDLRLREEMQTELKGLQQRLGITFVFVTHDQGEALSMADRVAVFSQGRIEQLDT
                     PRALYTRPRTAFVARFVGSANVVPTELARRLTGRDDAFAIRPELIDIVAAGAPLREDD
                     VACDGVVIDVLYHGASSRCRVQVDDGTILAVDRPEAAVAPPEAGSRVRLAWRREHAAA
                     LEA"
     gene            complement(5762..>6805)
                     /locus_tag="VD737_00230"
     CDS             complement(5762..>6805)
                     /locus_tag="VD737_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXW09015.1"
                     /translation="DIVAWPGYIERGDTDKSYDWVTGFESDTGCKVNIKTAGTSDEMV
                     SLMTQGGYDLVTASGDASLRLIRGGTVQPIDVARVPSFANVDERLQTAPWHYVDGKNY
                     GTPYQWGPNVLMYNTTTFPQPPKSWSVVFEEQRLPDGKSNKGRIQAYDGPIYIADAAL
                     YLMAKKPELGIKDPYELNEEQYAAVLDLLRKQHPLVHRYWHDANVAVQDFTNEGVVAS
                     GSWPFQVNTLVANKQPIASVVPQEGATGWADTTLLATDAKHPNCAYRWMEWSLNAKVQ
                     GDLAAWFGSVPAVPAACQGNALLGDGGCATNGFDDFDKIHFWRTPEAACAQGTCVPYS
                     RWATDYVAVMGGR"
BASE COUNT         1070 a         2374 c         2347 g         1014 t
ORIGIN      
        1 caccgggaca acaccatcgg cgccacaccg cagccgaacc ccggcgacga cgactgggtg
       61 cgattcttcc gggagcaccg tctcgcgcac atgctcgacc tcgccgcggc acgcgggctg
      121 ccttcgcgga ccgtcgaccg cggtcgggaa ctgatcgagc gctgcccggc actgttcgcg
      181 ggctaccggc cggtgccctc gctgcttcac ggcgacctgt ggggcggcaa ctggtcagcc
      241 cacgccctga cgtgcgaacc cgtcgtcttc gatccggccg tgtatcgcgg cgaccgcgaa
      301 gccgacctcg cgatgacaca cctgttcgga ggcttcggcc ccgacttcta cgccgcatac
      361 ggtgcgcagt ggccgctgga gccgggcgcc ggcgcgcgcc gcccgctgta caacctgtac
      421 cacgtgctga accacttcgt gctgttcggc ggcggctacg cgcggcaggc cgatgcgatg
      481 atcgggcggc tgctcgccga cctcgggtga ccggcggacc ggtcagggca gttgaacctt
      541 gcctttgacg cccgacacct tgacctcgcc cgaagagtca ctgagcaact ggaagtcgcg
      601 cccgacgtcc gccacgcgga tgtcgccgga ggaatcctgg tcgatgcgca cgctcccggc
      661 cacgcgccgg atcgagatgt ctccggacct gtcgctcggg attacgacct ctttcgcgcc
      721 gacgatcacg atgtcgccgg agctgtcgcg gacctgaacc tgccccgcgg cgtcctcgat
      781 ccgcacgtcg cccgagctgt cccagatctc gatgtcggcg cgggggtggc gcagcaggac
      841 gtcgcccgag ctgtcggtga gtttgacgat gcgcacgtcg gccgcgagca cgtcgccgct
      901 cgagtcggtg atctcgatcg gcagcgccgc gggcacgtcc acggtcaggt cgagcgtcgc
      961 gtagaagacg ccgatgccga ccatccggtc cggcaccagc acgtcgacgc gcagcgtgtc
     1021 gccctcgcgg cgcacggcga tgtcggtctg ttcgagaaac ttcgcgctgg acgcgcaggc
     1081 cggtcccgcc gcgacgagcg tggtgccgtc ggtgggcttc acctcgagat cccccgcccg
     1141 ggcgttgatc acgacgcgcg ccacgccggc cgtgtcgagg ctcgcccggc gctcggcccg
     1201 gtgctcgcac atgtcccagg ccgccgcggg cgcagccagg gccaacagcg ccaggccggg
     1261 caccaggagt cgaaccgggc ggaagggatc ggtacgcatg cgaagtcctc ggggcaggat
     1321 gtcgttggga tgcgccgggc ggccgccggg ttgcatcggc cgcgacaaaa acctgccgct
     1381 aacctctgtc gcccgtactt ccgcctcggc cggaggcagc cgagggtcag aacttcgcca
     1441 tcacgtggcg gaccacggtg tagtcctcga gcgcgtaggc ggacaggtcc ttgccgtacc
     1501 ccgaccgttt caggccaccg tgcggcatct cgctcaccag catgaagtgc gtgttgatcc
     1561 aggtgcagcc gtactgcagt cgtgaggcga cctcgagcgc ccggccgaca tcgcgcgtcc
     1621 agacggacga ggccagcccg tagtccgagt cgttggccca ccgcacggct tcgtccgcat
     1681 ccttgaaccg cgtgacggaa acgaccggtc cgaacacctc gcgctgcacg atctcgtccg
     1741 actgccgcgc attcgcgatc acggtgggct cgaagaagaa gccgcccccg ccgccggcat
     1801 ggccgccggc cgtgacttcg atgtgcttct gcttcttcgc gcgcgcgacg aagccggcga
     1861 cgcggtcgcg ctgcccggcg ctgatcagcg ggcccatttc gacgtccttg gacgtctgtg
     1921 ccccgacctt gatcgatcgc acggcgctcg acaggtccgc gacgagcctg tcgtacacct
     1981 tggcgcccgc gtagatccgg cacgccgcgg tgcagtcctg gcccgcgttg tagtagccga
     2041 acgttcgtac gcccgagacg acgctttcga cgtcggcgtc gtcgaacacg atcactggcg
     2101 ccttgccacc gagttcgagg tgcgtgcgcc ggatgccgtc ggccgcctgg ccgagaatct
     2161 tctcgccggt cgcgacgtct ccggtcaacg aaatcatgcg cacgagccgg tgccgggtga
     2221 gcggcgcacc gacgctgctg ccgcgaccac agacgacgtt gacgacgccc ggtgggaaga
     2281 tctcggcgag cagctccgcg agcttgagcg ccgtgagtgg cgtctgctcg gacggcttca
     2341 gcaccgcggt gttgccggcc gcgagcgccg gtccgagctt ccaggcggcc atcatcaacg
     2401 gatagttcca gggggcgatc gaggccacca cgccgatcgg gtcgcgcctg atcatgctcg
     2461 tgtagccggc caggtattcg cccgccacgg cgccggtcat cgtgcggcag gcgccggcga
     2521 agaagcggaa cacgtcggcg atcgccggga tctcgtcgtt gagcgccgcg gcgaacggct
     2581 tgccgcagtt ctgcgactcg agcttcgcga acgcggcgcc gtcggcctcg atgcggtccg
     2641 ccagcttgag cagcaccgcg gcacggtcct tgggtggcgt gcgcgcccag ccgtcgaacg
     2701 ccgcgtccgc cgccttgacc gccgcgtcga tctgcccgcg cgaggccgcc gcgaccttgg
     2761 caagcgactt gcccgtggcg ggatcgagga tgtcctcggg ctggccttcg cccttgacga
     2821 ggtttccgtt gatgagcagt ttggtctgca tggctagtcc tcgctcgacg ccaagtcgga
     2881 aaaagagtgg ctagtggtca gtggctagtg gctagtgttc agacgcagcc gccggcgttg
     2941 gcgcggaggc gtctaatact gaccactagc cactgaccac tagccacttt ccttctccgg
     3001 ccggctcagc caatacgccc ccaggatcgg cagcaacgtc accaggatca cgatcacggc
     3061 gaccacgttg gtgaccggtc gctcgcgcgg ccggaacagc tcgttgtaga accagatggg
     3121 cagggtccgc tcgtgccccg aggtgaacag cgtgacgatg atctcgtcga acgacagcgc
     3181 gaaggcgagc atgccaccgg ccagcagcgc gctcgcgatc tgcggcagca ggacgtaccg
     3241 gaaggtctgg tgcccgcgcg cgccgagatc gcccgaggcc tcgacccagc tgaccgggat
     3301 gcggcgcagg cgcgcgatca cgttgttgta gacgatcacg aggcagaacg tcgcgtgtcc
     3361 ggcgacgatg gtccagaagc caggttcgac accaagcgtg cggatgccgg cgagcagcgc
     3421 cagcccggtc aggataccgg gaagggcgat cggcagcacg aacagcagcg tgatcgactc
     3481 cttgccgcgg aacgtgcgtc gcgcgagggc gaacgccgcg agcgtgccga ggacgagcgc
     3541 gacgagcgtc gcgaccgtcg cgaccagcag cgacagccgc atcgcggccc agatgtcgct
     3601 gcgagcgaac gccacttcga accagtgcag cgtcagaccc ggtggcggga actgatacgt
     3661 tcgatcctcg gtcgtgaacg cgtacagcgc gatcacccac accggcacgt gcaggaacag
     3721 caatccgagc gccgcgaacg cgaccgtgag ccgtgacgcg cgctgccagc gtggcttgcg
     3781 gcgggattca gagcgcatcg aacgccccga gccggcgcgc gagcgcgagg tacgcaccga
     3841 tcagcacgat cggcacgacc gagaaggcgg ccgccagcgg caggttcccg gccgtgccct
     3901 gctgctggta gaccatcatg ccgatgaagt agcccggcgc gccgaccacg ctcgggatga
     3961 tgtagtcgcc gagcgtgagc gagaacgtga agatcgagcc ggccgccagt cccggcagtg
     4021 cgagcggcag cacgacgtgc cggaaggtct gtcgcggcgt cgcgcccagg tccgccgacg
     4081 cctgcagcag cgagtccggg acgcgctcca gcgccgccgc gagcggcagg atcatgaacg
     4141 gcagccacat gtacacgaac accacgaaca tgccgaggta cgacgaggcg agcgaagggc
     4201 cgccgaccac cggcaccgcc agcacgcggt cgagcgcacc ggcgagcccg acctgctcga
     4261 ggctccagct cgcgatgccc tgtttcgcca ggatcaggcg ccacgcgtag accttgacca
     4321 gatagctggt ccacatcggc agcaggattc cgacgtagaa cagcgccttg agacgggggc
     4381 ccgcgtgccg cgccatgtac agcgcgaccg gaaacgcgat cacggccgac gcgatcgtca
     4441 ccgcaacggc catcgccagc gtgcgcagga tgatgtcgag gtgcgcgggc cgcgtcagca
     4501 gcacttcgta cgtcgcgagc gtcggctcgg gcacgacacg ggccgtgaac tcgtcgatcg
     4561 catagaagct ctgcatcagg agcgtgaaca gcgagccgag gtagacgatg ccgagccaca
     4621 gcaggggcgg tgcgagcagc agcgcgaaat agaacgcacg gcggcccccg agcgacatcg
     4681 cggggagccg cgccacgctc aagcctcgag cgccgcggcg tgttcccggc gccaggccaa
     4741 ccgcacgcgc gaacccgcct cgggcggcgc gacggcggct tccggacgat cgacggcgag
     4801 gatcgtgccg tcgtcgacct ggacccggca gcggctgctc gccccgtgat acagcacgtc
     4861 gatgacgacg ccgtcgcagg cgacgtcgtc ctcgcgcagc ggcgcacccg cggcgacgat
     4921 gtcgatcagc tccggccgga tcgcgaaggc gtcgtcgcgc ccggtcaggc gacgtgcaag
     4981 ttcggtggga acgacgttgg cgctgccgac gaacctggcg acgaacgccg tgcgagggcg
     5041 cgtgtacagc gcacgcggcg tgtcgagctg ctcgatccgg ccctggctga acacggcgac
     5101 gcggtccgcc atgctgagtg cctcgccctg gtcgtgcgtg acgaacacga acgtgatgcc
     5161 gagccgttgc tgcaggccct tgagttccgt ctgcatctcc tcgcgcagcc tgaggtcgag
     5221 cgcgcccagc ggctcgtcga gcaacagcac cttcggccgg ttgatcagcg cgcgcgccag
     5281 cgctacccgc tgcctctgcc cgcccgaaag ctcgtacggc cggcgcgcgc cgagttcgcc
     5341 gaggcgtacc agttcgagca tttcgcgggc gcgcgaccgg cgctcgctcg cgtcgatgcc
     5401 gcggatccgc agcccgtagg cgacgttgtc ggcgacgctc atgtgcggaa acagcgcgta
     5461 gtcctggaac accgtgttga cgtcgcgggc gtacggcggc gtgtccgtga cgtcctcgcc
     5521 gccgagcagc acccgcccgg cgtcgggctg gtcgaagccg gcgatcagcc tcaggcaggt
     5581 cgtcttgccc gagccggacg ggccgagcag cgcgaagaac tcgcctggcg cgatcgcgaa
     5641 cgacacgcga tcgagcgcga cgaccgcacc gaaagtgcgg ccgacgtccc gcaactcgat
     5701 ggcggccgtc attcggctcg gcgccgtgcg gtgatccgcc gatggtcggc cgtcgcgtgc
     5761 ctcagcgccc gcccatcacg gcgacgtagt cggtggccca gcggctgtac ggcacgcagg
     5821 ttccctgcgc acacgcggcc tcgggcgtcc gccagaaatg gatcttgtcg aagtcgtcga
     5881 agccgttcgt cgcgcagccg ccgtcgccga gcagggcgtt gccctggcag gccgccggca
     5941 cggccggcac ggagccgaac caggccgcca ggtcgccctg gaccttggca ttgagcgacc
     6001 actccatcca gcggtaggcg cagttcgggt gcttcgcgtc ggtggcgagc agggtcgtgt
     6061 cggcccaccc ggtggcgccc tcctggggca ccacgcttgc gatcggctgc ttgttggcca
     6121 cgagcgtgtt gacctggaac ggccacgagc ccgaggcgac gacgccttcg ttggtgaagt
     6181 cctgcacggc gacgttggcg tcgtgccagt agcggtgcac gagcgggtgc tgcttgcgca
     6241 gcaggtcgag cacggccgcg tactgctcct cgttgagctc gtacgggtcc ttgatgccga
     6301 gctccggctt cttcgccatc aggtacaggg cggcgtccgc gatgtagatc gggccgtcgt
     6361 aggcctggat gcggcccttg ttgctcttgc cgtccgggag cctctgctcc tcgaacacga
     6421 ccgaccagga cttcggcggc tgcgggaacg tggtcgtgtt gtacatcagc acgttcgggc
     6481 cccactgata gggcgtgccg tagttcttgc cgtccacgta atgccacggc gccgtctgca
     6541 gtcgctcgtc gacgttggcg aagctcggca cgcgcgcgac gtcgatcggt tgcaccgtgc
     6601 cgccgcggat cagccggagc gacgcgtcgc cggaggccgt gacgaggtcg tagccgccct
     6661 gcgtcatcaa cgagaccatc tcgtcggacg tgccggcggt cttgatgttg accttgcagc
     6721 ctgtgtccga ttcgaacccc gtcacccagt cgtagctctt gtcggtatct ccacgctcga
     6781 tgtagcccgg ccaggcgacg atgtc
//
LOCUS       DASPFR010000010         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_369840, whole genome shotgun sequence.
ACCESSION   DASPFR010000010 DASPFR010000000
VERSION     DASPFR010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369840"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VD737_00235"
     CDS             <1..846
                     /locus_tag="VD737_00235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXW09016.1"
                     /translation="HNMAGVASAATSVVIGYIAGGTRTIRVGAGGIMLPNHSPLVIAE
                     QFGTLARLFPGRIELGLGRAPGTDQETVRALRRTPGAAERFPDDVVEVQRFFEPAAAG
                     QRVQAVPAAGTRVPLWILGSSTYGAALAAELGLPYAFASHFAPQQLMTALHVYRSRFR
                     PSEQLARPCAMVGANVIAADTDEEARRLATTQQMAFVDLVRGTRSLSKPPIDDIEQYW
                     TPVEKAHAMQMLAMSVVGSRATVRAGVESLVQQTGTDELIVVSDVYELKARLRSFELI
                     ADVRS"
     gene            complement(892..>2481)
                     /locus_tag="VD737_00240"
     CDS             complement(892..>2481)
                     /locus_tag="VD737_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXW09017.1"
                     /translation="GRRFGPDEQTGVRGNVAYRDGETSVEDQERELTVLAFGIDHDGE
                     NFRLSADIGYQDHHIDAPRPSVTPLGGIPKPPDADSNFAQPWTYTDEEQLFGVVRAEY
                     DFTRAVSAWAAYGLRQGEEANVLANPSALPDGSTSSYRFDNTREDDVSSGEIGLRWDF
                     ATGPIEHRIILSASIFHIESNNAYAFSEFFPGFASDLYDPVEVAPPAATFFTGGNLNN
                     PGKTFETETSSLAIADMMTFADGKFILTLGARDQDLKSDNFDYNSGAKLSGYDESELT
                     PVGGIVFRPNDSVSLFANYIEGLVAGDTAPFTGPAPGFLPVTNAGEIQDPIAAEQIEL
                     GVKFESASFGGSLSLFNIEKGVGALAADPDTVTDDLRYSISGEVRHRGVEAGLYGQPL
                     ENVRVLGGVTWLDAEYTEGGVGLDEGNQPIGTPEVQANVNVEWDLAALEGVTLEARAL
                     YTSSQYADTANTLELDSWTRFDIGARWSTELAGRPFSLRARVDNVTDENDWISAGGFP
                     GFGYLVLGAPRTFVVSASMDF"
BASE COUNT          375 a          833 c          872 g          401 t
ORIGIN      
        1 cacaacatgg ccggcgtggc gagtgcggcc acgtccgtcg tcatcggcta catcgcgggc
       61 ggcacgcgca cgatccgcgt gggcgcgggc ggcatcatgc tgccgaatca ctcgccgctc
      121 gtgatcgccg aacagttcgg cacgctggcg cggctgttcc cgggacgcat cgagctcggg
      181 ctcggtcggg cgccgggcac cgaccaggaa accgtccgcg ccctgcgccg cacgccgggc
      241 gccgcggagc gcttccccga cgacgtcgtc gaagtgcagc gcttcttcga acccgctgcg
      301 gctgggcagc gcgtgcaggc cgtgccggcg gccggcacga gggtgccgct gtggatcctg
      361 ggttcgagca cgtacggcgc ggcgctggcc gccgaactcg gcctgcccta cgcgttcgcg
      421 tcgcacttcg cgccgcagca gctcatgacg gcgttgcacg tgtaccgcag tcgcttcagg
      481 ccgtccgagc aactggcccg gccctgcgcg atggtgggtg cgaacgtgat cgcagccgac
      541 accgacgagg aagcgcgccg gctggcgacg acgcagcaga tggcgttcgt cgacctggtc
      601 cgcggcacgc gttcgctcag caagccgccg atcgacgaca tcgagcagta ctggacgccg
      661 gtcgaaaagg cccacgcgat gcagatgctc gcaatgtcgg tcgtcgggtc gcgcgcgacc
      721 gtgcgggcgg gcgtcgagtc gctcgtgcag cagaccggca ctgacgagct gatcgtcgtg
      781 tccgacgtct acgaactgaa agcgcggctc aggtcgttcg aactcatcgc ggacgtgcgg
      841 tcatagtccg cggccgacct ctcccgctcg cgcgggagag gtcgagcggg ttcagaagtc
      901 catcgacgcc gagacgacga acgtgcgcgg agcgccgagc acgaggtagc cgaagcccgg
      961 gaaaccgccc gccgagatcc agtcgttctc gtccgtgacg ttgtcgaccc gggcgcgcaa
     1021 cgagaacggc cggcccgcga gttcggtcga ccagcgcgcg ccgatgtcga accgcgtcca
     1081 cgagtcgagc tcgagcgtat tggccgtgtc ggcgtactgg gacgacgtgt acagcgcgcg
     1141 cgcctcgagt gtgacgccct cgagcgccgc caggtcccat tcgacgttca cgttggcctg
     1201 gacctcgggc gtgccgatcg gctggttgcc ctcgtcgagt ccgacaccgc cctcggtgta
     1261 ctcggcgtcc agccacgtca cgccgccgag cacgcggacg ttctcgagcg gctggccgta
     1321 gagaccagcc tcgacaccgc ggtgacgcac ttcgccgctg atggagtacc tgaggtcgtc
     1381 ggtaacggta tcggggtccg cggcgagcgc accgacaccc ttctcgatgt tgaagaggct
     1441 cagcgagccg ccgaagctcg cggattcgaa cttgacgccg agctcgatct gctcggcggc
     1501 gatcgggtcc tggatctcgc ccgcgttcgt gaccggcagg aagcccggcg ccggtcccgt
     1561 aaacggtgcc gtgtcgcccg cgaccaggcc ttcgatgtag ttcgcgaaga gcgataccga
     1621 gtcattgggc cggaacacga tgccgccgac cggcgtgagc tcgctctcgt cgtagcccga
     1681 gagcttcgcg ccggagttgt agtcgaagtt gtcgctcttg aggtcctggt cgcgtgcacc
     1741 gagcgtcagg atgaacttgc cgtcggcgaa cgtcatcatg tccgcaatgg ccaagctcga
     1801 ggtctccgtc tcgaaggtct tgcctgggtt gttcaggttg ccaccggtga agaacgtggc
     1861 ggcgggcggc gccacttcga ccgggtcata caggtcgctg gcgaaacccg gaaagaactc
     1921 cgagaacgcg taggcgttgt tcgactcgat gtggaatatc gacgccgaca ggatgatgcg
     1981 gtgctcgatc gggccggtcg cgaagtccca gcgcaggccg atttcgcccg acgacacgtc
     2041 gtcctcgcgg gtgttgtcga accggtagga gctcgtgctg ccatcgggga gggcgctcgg
     2101 gttggcgagc acgttcgctt cctcgccctg gcgcaggccg taggcggccc acgccgagac
     2161 tgcgcgcgtg aagtcgtact ccgcacgcac gacgccgaac agctgctcct cgtcggtgta
     2221 ggtccacggc tgcgcgaagt tcgagtccgc gtcgggcggc ttcgggatgc cgccgagcgg
     2281 cgtcacgctc ggccgcggcg cgtcgatgtg gtggtcctgg taaccgatgt cggccgagag
     2341 ccggaagttc tcgccgtcgt ggtcgatgcc gaacgcgagg acggtgagct cgcgctcctg
     2401 gtcttcgacc gacgtctcac cgtcccggta cgcgacgttg ccgcgcacgc cggtctgctc
     2461 gtcgggaccg aaccgccgac c
//
LOCUS       DASPFR010000011         5558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1346726, whole genome shotgun sequence.
ACCESSION   DASPFR010000011 DASPFR010000000
VERSION     DASPFR010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346726"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VD737_00245"
     CDS             <1..991
                     /locus_tag="VD737_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXW09018.1"
                     /translation="YASAAADAEYARQSVAAMTAKAYFLAVEAGLQQRLAEEMLGAST
                     KLVELSEQRERVGKGDGFDSAIARGNVEGFRDTIEQLRFARERALRALEALLGRYPSA
                     SLDVATELTAVPGPVPAGLPSELLERRPDVIAAERRVAAAFNRAQEARAARLPRISLT
                     ASVNDISSDLFVLKNRDDPVWSAGATLMAPVFSGGALRQQVRIRTAEQRQAVAEYGQV
                     GARAFGDVEDALSAEFAAKRREAVLARAVSENLRAADLAQVRYRVGSGDLLGVQQRQL
                     SVYGARSTLVRVQTEQLVQRVNVYLALGGGFDTAQASTQSATVSASAGASVAP"
     gene            1049..1708
                     /locus_tag="VD737_00250"
     CDS             1049..1708
                     /locus_tag="VD737_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXW09019.1"
                     /translation="MKTTAKWLAVAAGLALALPGHAEPLEQGDWEASLGVVFLNSSEA
                     DFKGGSAADFDSDSGFRGAFDYSVTDALQVGASFSIGQRSYDASIAGDEPGESFRARG
                     DLDYSSLLATATFNLLDGPFRPFVTAGIGWSWIDTNIATEPPEVGCWWDPWYGYICTS
                     WVNTRTLDGLTYQLGVGARYDFANNVAVHASYRIDWIDFDQADGTPDFDGFDLSIGFK
                     F"
     gene            1761..2978
                     /locus_tag="VD737_00255"
     CDS             1761..2978
                     /locus_tag="VD737_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09020.1"
                     /translation="MSTGQPVAEIGALRTASRDDRYFSLVLGGPLYQLLRRSRLSDDH
                     LMLQRRRIVVITTLAWLPLLLLAAASGTLTSGVAVPFLMDVEPHTRFLLALPLLIVAE
                     VTVHERMLLVASNFRDRGLIPPAAETQLEAAVTSAYRWRNSVGAEVGLLALVYAVGIS
                     IVWRDYLSLPTATWYATPDGDGSTLTLAGAWYGYVSLPLFQFLLVRWYFRIAIWARLL
                     WHVSRIELQLVPTHPDRVGGLGFLSNTVFAFIPLILAHGVLLAGVLANHIFHTGAKLT
                     DFRLEILLMVILLTLLVVGPLAVFAAQLARAKRLGLREYGSFAQRYVRAFDSKWLRAQ
                     EPEDALLGSGDIQSLADLANAFGVVKEMRLVPISKEAIFQLAVVALAPIVPLLLTLMP
                     LDELLRKLIGVVF"
     gene            2984..3982
                     /locus_tag="VD737_00260"
     CDS             2984..3982
                     /locus_tag="VD737_00260"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HXW09021.1"
                     /translation="MPRVNDLFPGLVVAGMVALAASWLAEHYATPVMLFALLLGIAVN
                     FLSQHEQCRPGLDFASRSLLRIGVALLGARITLTQVGSLGGAALLLTAAAVVLTILCG
                     LMLARATRQSAAFGLLSGGSVGICGASAALAIASVLPRSDALERDTVMTVVAVTALST
                     VAMVLYPVVAAGFGLDERTAGIFLGATIHDVAQVVGAGYSVSTPAGDAATIVKLFRVA
                     MLLPVVLVISFALQRRRTEASGAMQGDAPSLLPAFLVVFASLVAVNSLGWLPDALTSS
                     LQEASRWSLVVAIAALGTKTSLAELAGVGWRPVAIIVTETAFVGLFVLAGLSLLGA"
     gene            complement(4019..5098)
                     /locus_tag="VD737_00265"
     CDS             complement(4019..5098)
                     /locus_tag="VD737_00265"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HXW09022.1"
                     /translation="MSMLQETVHRRAHSVRVAVLTSVAACCSLAFGPVGAAPKTDVVD
                     LVNGDRITGEIKGMEHNRLRLSTDHMGTIYIEWDKIARLRSDQYLLLERFDGVRYYGQ
                     LVQGADQAHLQVRRDPQGQAEPVSMSSVVRAQPILGGDFIDRLDGYVSAGLDTAKASD
                     RRAFDFAGGLSSRTRVREWKVDGSVNLTDDDADETSERYHLQGSRRQFRRDRDFSLGY
                     GVLDRNTELDLNLRALVGAGYGRYFVQSNSAEWVGGAGLAYSHENYTGGESLDSLEAL
                     LTTSFSIFRYDFPETDIAGSLSLLPSLTESGRYRAEADLRARYEFVDDLYFELKLYGS
                     YDSEPPLEESEQSDYGVVTSLGYSF"
BASE COUNT          843 a         1893 c         1836 g          986 t
ORIGIN      
        1 ctacgcttcg gccgcggccg acgccgagta tgcccgtcag tccgtggcgg cgatgacggc
       61 caaggcgtac ttcctcgccg tggaagcggg cctgcagcag cgactcgccg aggagatgct
      121 cggcgcatcg acgaagctcg tggagctctc cgagcagcgc gagcgcgtcg gcaagggcga
      181 cgggttcgac tccgcgatcg cccggggcaa cgtcgagggc tttcgcgaca cgatcgagca
      241 gctgcgcttt gcacgcgagc gggcactgcg tgcgctcgag gcgctgctgg gacgctaccc
      301 gtcggcctcg ctcgacgtcg ccaccgagct cactgccgtc ccgggcccgg tgccagcggg
      361 attaccgtcc gagctcctcg agcgccgccc ggacgtgatc gccgccgagc ggcgcgtcgc
      421 ggcggcgttc aaccgcgccc aggaagccag ggccgcgcga ttgccgcgca tctcgctgac
      481 cgcgtcggtc aacgacatct cgagcgacct gttcgtgctc aagaaccgcg acgaccccgt
      541 gtggagtgcc ggcgcgaccc tgatggcccc ggtcttcagc ggtggcgcgc tgcgtcaaca
      601 ggtgcgcatc cgcacggccg aacaacgcca ggccgtcgcg gaatacggcc aggtcggcgc
      661 gcgggccttc ggcgacgtcg aagacgcctt gtccgccgaa ttcgccgcca agcgacgcga
      721 ggccgtgctg gcgcgggcgg tcagcgaaaa cctgcgcgca gccgacctcg cgcaagtccg
      781 ttatcgcgtc ggttccggcg acctgctcgg cgtgcagcag cgccagctgt cggtctacgg
      841 tgcgcgttcg acgctcgtgc gcgtgcagac ggagcagctc gtacaacgcg tcaatgtcta
      901 cctcgcgctc ggcggcggtt tcgacaccgc ccaggcctcg actcagagcg cgactgtgtc
      961 tgcgagcgcg ggggcatcgg tcgctccttg aacgcaggcg cccacggatt tgggggaaac
     1021 atgagcaagg aaacgaagga gtgatgacat gaagacgacc gccaaatggc tggccgtggc
     1081 agccgggctg gccctggcac tgccaggcca cgccgagccg ctcgagcagg gcgactggga
     1141 agccagtctc ggcgtggtgt tcctgaattc ttccgaggcc gacttcaagg gcggttccgc
     1201 ggcggatttc gacagcgatt ccggattccg cggcgcgttc gactacagcg tcaccgacgc
     1261 gctgcaggtc ggcgccagct tctcgatcgg ccagcgcagc tacgacgcat ccatcgccgg
     1321 tgacgagccg ggcgagagct tccgagccag gggtgacctc gactattcct cactgctggc
     1381 cacggcgacg ttcaacctgc tcgacgggcc gttccggccg ttcgtgaccg ccgggatcgg
     1441 ctggagctgg atcgacacca acatcgcgac cgagccaccg gaagtcggct gctggtggga
     1501 tccctggtac ggctacatct gcacgtcatg ggtcaacacg cgcacgctcg acggactgac
     1561 ctaccagctc ggcgtcgggg cgcgctacga cttcgccaac aacgtcgccg tgcacgcgag
     1621 ctaccggatc gactggatcg acttcgacca ggccgacggc acgccggatt tcgacgggtt
     1681 cgacctgagc atcgggttca agttctgacg ccgcagggcg ccctccggtt acggagggcg
     1741 ctcactgcgg aggggaagcc atgagtaccg gacaaccggt tgcagagatc ggcgcgctgc
     1801 gaacggcgtc gcgtgacgac cggtatttct cgctggtgct cggcgggccg ctctatcaac
     1861 tgctgcgtcg gtcgcgcctg tccgacgacc acctgatgct gcagaggcgc cgcatcgtcg
     1921 tcatcacgac gctcgcctgg ctgccgctgc tgctcctggc cgcagcaagc ggcacgctca
     1981 ccagcggcgt ggccgtgccg ttcctgatgg acgtggagcc gcacacccgt ttcctgctgg
     2041 cgctgccact gctgatcgtc gcggaggtca cggtccacga gcgcatgttg ctcgtcgcga
     2101 gcaattttcg cgatcgcggc ctgatcccac ccgcggccga gacacagctc gaggcggcgg
     2161 tcacctccgc ctaccgatgg cgcaactcgg tcggcgccga agtcgggctg ctcgcgttgg
     2221 tctacgcggt cggcatctcc atcgtgtggc gcgactacct ttcgctgccc acggccacct
     2281 ggtacgcgac tcccgacggc gacggctcga ccctgacgct ggccggggcc tggtacggct
     2341 atgtgagcct gccgctcttc cagttcctgc tcgtccgctg gtatttccgc atcgccatct
     2401 gggcgcgact cctgtggcac gtctcgcgga tcgagctgca actcgtgccg acgcatcccg
     2461 atcgcgtcgg cggcctcggg ttcctctcga acacggtgtt cgcgttcatt cccctgatcc
     2521 tcgcgcacgg cgtactgctc gccggcgtgc tcgccaacca catcttccac accggtgcca
     2581 agctgaccga tttccgcctc gagatcctgc tgatggtcat cctgctcacg ctgctggtcg
     2641 tcggcccgct cgccgtcttc gccgcccagc tcgcgcgggc caagcgcctg gggttgcgcg
     2701 aatacggcag cttcgcgcag cggtacgtgc gcgccttcga cagtaagtgg ctgcgcgcgc
     2761 aggagccgga ggacgcgctg ctcggctcgg gggacatcca gtcgctggcg gacctggcga
     2821 acgccttcgg agtcgtgaag gagatgcgcc tcgtgcccat ctccaaggaa gcgatcttcc
     2881 agctcgcggt cgtggcgctg gcgccgatcg tgcccctgct gctcacgctg atgccgctcg
     2941 atgagctgct gcgcaagctg atcggggtcg tcttctgaag tcgatgcccc gcgtcaatga
     3001 cctgtttccc ggcctcgtcg tggccgggat ggtggcgctg gccgcctcgt ggctcgcgga
     3061 gcattacgcg acgccggtga tgctgttcgc gctgctgctg ggcatcgccg tcaatttcct
     3121 ctcgcagcac gagcaatgcc ggcctggcct cgacttcgca tcgcgcagcc tgctgcgaat
     3181 cggcgtggcg ctgctcggcg cgcgcatcac gctgacgcag gtcggatcgc tgggcggcgc
     3241 cgcgctcctg ctcactgccg ctgccgtggt gctgacgatc ctatgcggcc tgatgctcgc
     3301 gcgcgcgacc cggcaatcgg ccgctttcgg cctgttgtcg ggaggatcgg tcggcatctg
     3361 cggcgcgtcg gctgcgctcg cgatcgcctc ggtgctgccg cgcagcgacg cactggagcg
     3421 cgacacagtc atgaccgtcg tcgccgtcac ggctctctcc accgtcgcca tggtgctgta
     3481 cccggtcgtc gcagctggat tcggcctcga cgaacgcacg gccggcatct tcctcggcgc
     3541 gacgatccac gacgtcgccc aggtcgtcgg cgcgggttac agcgtgtcca cgcctgcggg
     3601 cgatgccgcg accatcgtca agctgttccg cgtcgccatg ctgttgccgg tcgtgctcgt
     3661 gatctccttc gcattgcagc gccggcgcac cgaggcttcc ggagcgatgc agggcgacgc
     3721 tccgtcgttg ctgcccgcct tcctggtcgt gttcgccagc ctcgtggcgg taaattcgct
     3781 cggctggctg ccggacgccc tgacctcgtc gctgcaggag gcgtcaaggt ggagcctcgt
     3841 cgtcgcgatt gccgcgctcg gcacgaagac gtcactcgcg gaactcgcgg gcgtcggctg
     3901 gcgacctgtc gcgatcatcg tgaccgaaac ggcctttgtc gggctgttcg tgctcgcggg
     3961 cctgtcgctg ctgggagcgt aggcggcgtc accgacgaga ccgcaacgag ttgcgcggtc
     4021 agaagctgta gccgagcgaa gtcaccacgc cataatcgct ctgttcggac tcctcgaggg
     4081 gtggctcgct gtcgtagctg ccgtacaact tgagttcgaa gtacaggtcg tcgacgaact
     4141 cgtatcgcgc ccgcaggtcg gcctccgcgc ggtagcgacc ggactccgta aggctcggca
     4201 acagggacag ggatccggcg atgtcggttt cgggaaagtc gtagcggaag atcgagaacg
     4261 acgtcgtcag cagggcctcg aggctgtcga gcgattcgcc cccggtgtag ttttcgtgtg
     4321 agtacgccag acccgcgccg cctacccact ccgccgagtt cgactgcacg aaataacgac
     4381 cgtagcccgc gccgacgagc gcgcgaaggt tgaggtcgag ctcggtgttg cgatcgagca
     4441 cgccgtagcc cagggaaaaa tcgcggtcac ggcgaaattg ccgtcgcgaa ccctggaggt
     4501 gataccgctc gctggtctcg tcggcgtcgt cgtcggtcag gttgacagac ccgtccactt
     4561 tccactcgcg gacgcgtgtg cgtgacgaca ggccgccggc aaaatcgaag gcgcgacgat
     4621 cgctcgcctt ggccgtgtcg agacctgcgc tgacgtagcc gtccaggcgg tcgatgaaat
     4681 caccgcccag gatcggttgg gcgcgcacga cgctggacat cgagactggc tccgcctggc
     4741 cttgcggatc ccgccgcacc tgaaggtggg cctggtcggc gccctgaacc agctggccgt
     4801 aatagcggac accgtcgaac ctttcgagca acaggtattg atccgatcga aggcgtgcga
     4861 tcttgtccca ctcgatgtag atggtgccca tgtggtccgt gctgagccgc aggcggttgt
     4921 gctccatgcc cttgatctcg cccgtgatgc ggtccccgtt gacgaggtcc acgacgtccg
     4981 tcttcggcgc cgcgccgacc ggcccgaacg cgagcgagca gcatgctgcg acgctcgtga
     5041 gtaccgcgac ccgcacggag tgcgcccggc ggtgaaccgt ctcctgcagc attgacattt
     5101 gcactccctc cgccccgtcc ttatcacgag cacccggctg cacccctccg attctcgtgg
     5161 tcgaatgctg gccgcggcaa cctcgacgac catcgcttga tgatcggtcg gatcaaaggt
     5221 ggccatcctc tgcacgcgcg cagtacgcga tcatgcctat caaccctcgg tggctcctca
     5281 tggcctttgt cacttgcgca aaagccatta ccaggcggca cgcctacact tttaagcaag
     5341 tagtcagacc gagcgtttga cggatcgatc gaaatgaggc gcccttacgt aggcgccctc
     5401 agatggaagg cgcaaagaaa caacagcctt gcatcgtacg gtggcgccgg gcggccgcca
     5461 cggtaagtcg acatctcaca tggagccgat gacatgacaa cttgctggtt cggcaggcac
     5521 tgcctgtggg ccggggcgat cattgcggtc gggataat
//
LOCUS       DASPFR010000012         4391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1469254, whole genome shotgun sequence.
ACCESSION   DASPFR010000012 DASPFR010000000
VERSION     DASPFR010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469254"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..803
                     /locus_tag="VD737_00270"
     CDS             204..803
                     /locus_tag="VD737_00270"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HXW09023.1"
                     /translation="MHALSQEVLRTDVSGMPLEWIDYRDAVRLHFMGQVAYAFGQTLF
                     SLRGGVNARTGHRTTVQVSSIVATTGHSHNPGNRHHGYVPPLNNETLFRRDAHLCLYC
                     GQRFPSGQLSRDHVTPLSRGGHDTWTNVVTACRRCNNLKAWRTPEQAKLQLIAVPFTP
                     SYAEYIYLKGRRVLADQMEYLLAHFPRSSPLHDRLRKSV"
     gene            complement(1178..2089)
                     /locus_tag="VD737_00275"
     CDS             complement(1178..2089)
                     /locus_tag="VD737_00275"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXW09024.1"
                     /translation="MATSLKTPPLRLLRAFCLAARHSSFKVAADRLALTPSAVSHQVK
                     ELEDQLGVSLFQRRTRAVVLTTAGRQFLDDLEPALEAVAAAVARVARGGHARRQLMVV
                     MPPFFASELFAPRLPDFQQSHPDIDLQVQTTDPRPGQHSGFSDLSVILAGDVPSEPDV
                     QAVRLLPLQLVPVASPKVAASMKGKPRAQAFDRQTLIMHHSFRTSVWNDWLARADIDL
                     ARLKNLVEFDNMTAVARATERDGGIALMPELICQPWFDRGALVRIDGFDLEADEAYYL
                     VARHQDIERSEVRAFTQWLIEQFQVPA"
     gene            complement(2249..2797)
                     /locus_tag="VD737_00280"
     CDS             complement(2249..2797)
                     /locus_tag="VD737_00280"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXW09025.1"
                     /translation="MATVAMPSDLDESAPCRIVPVGREHLAGFHSALDRIAREGRYLA
                     MLQAPPFSRTRRFVLDSLRDGAIHYVAVAGDGTVVGWCDLRPKAAATLRHSAVLGMGV
                     VPEFRGRGVGTRMLAATLAGADARGLRRAELVVRADNHAAIALYRRFGFVEEGRCRQY
                     LQVDGEDHDALLMARLVSELRR"
     gene            3000..3890
                     /locus_tag="VD737_00285"
     CDS             3000..3890
                     /locus_tag="VD737_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HXW09026.1"
                     /translation="MLYLVDASVFIFRAWHSVPVDVSDRDGNPVNAVFGFARFVCELL
                     ERESPRHIAVAFDESLEAGYRNEIYPAYKANRDPAPIELQRQFALCREICAALGLAQY
                     GSSRYEADDIIGTLATRARVAGLPVTIVSRDKDLTQLLSPVDVYWDAIADVRYGYADI
                     EARFGVIPERMADFLALTGDAVDNVPGVPGIGRKTAQKLLKHFDTLHGVFENLEQVRK
                     LKFRNAVFVADSLRQHRDAAFLSRRLTAIACDMPLPISRVGDLRRRAPDLSTLDTFCE
                     TQGFGRVLRDQARRIYARLG"
     gene            3909..>4391
                     /locus_tag="VD737_00290"
     CDS             3909..>4391
                     /locus_tag="VD737_00290"
                     /inference="COORDINATES: protein motif:HMM:NF015818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HXW09027.1"
                     /translation="MDKEPSDAAIGAWLGAATGRRFESRPAETVHGGSIHHCVRWPAR
                     GGDAFVKLGAAEAIATFEAEVDGLEALREARALRVPEVLAAGVAAGRAVLALEWLDLS
                     PQVAERSSVQARLGEALAAQHRVTGPTWGWHRDNTIGATPQPNPGDDDWVRFFREHRL
                     A"
BASE COUNT          683 a         1514 c         1522 g          672 t
ORIGIN      
        1 cgatcgtcag tggcatggtg acctcatcga tgcttgggta cagggctgcc ggccgcgtcc
       61 cgccggactc tgcggcgggg cccgggcagc gtcacaggcg gtgctccctc ggccggactg
      121 cgtctaaagg cgatgccgaa ccggccgata ttctctggcc tgcgatgatg gacgtggcgc
      181 gggattatac gtatcgtcaa accatgcacg cactgtcgca ggaggtcctg cggacggacg
      241 tctcgggaat gcccctggag tggatcgact accgggacgc ggtacgtctg cacttcatgg
      301 gacaggtcgc gtacgcgttc gggcagacgc tgttctcgct gcgtgggggt gtcaacgcgc
      361 ggaccgggca tcgcacgacc gtgcaggtca gctcgatcgt cgcgaccacg ggccacagcc
      421 acaaccccgg caaccggcac cacgggtacg tcccgccgct caacaacgag acgctgttcc
      481 gccgcgatgc gcacctctgc ctgtactgcg gccagcgctt cccgtccggc cagctctcac
      541 gcgaccacgt cacgccgctg tcgcggggcg ggcacgacac ctggaccaac gtcgtcaccg
      601 cctgccggcg ctgcaacaac ctcaaggcgt ggcgcacgcc cgagcaggcg aagctgcagc
      661 tgatcgccgt gccgtttacg ccgagctacg ccgaatacat ctacctgaag ggccggcgcg
      721 tgctcgccga ccagatggag tacctgctcg cgcacttccc gcggtcgagc ccgctgcacg
      781 acaggctgag gaagagtgtt tagcggttag cggttagcgg ttagtcgcac ggcgcgtctc
      841 gcgcccatgt accgaaaaaa ggcgcccgag ggcgcctttt actttctacg gccggaacgg
      901 ccgttcctac taaccgctag acactaaccg ctagccactt cccaataact tgccggcgtg
      961 actgggagcc gcagggggag tgcgccccag tcacccggca agttccgtcg gatgggggaa
     1021 gcgccggagt cgcaggggga gtgcactcgg gacgcttcat tggccgccgg acgcaatcga
     1081 gactacgtat attgctgcac tgcgacaaac gagcgttggt catgtatggg tgagcgctgc
     1141 tcatccatcg gtgccgcgga aaaccacgcg ggcggcttca ggcggggacc tggaactgct
     1201 cgatcagcca ctgcgtaaac gcgcgcacct cggagcgctc gatgtcctgg tgccgtgcca
     1261 cgaggtaata ggcctcgtcg gcctcgaggt cgaacccatc gatgcgcacg agcgcgcccc
     1321 ggtcgaacca aggctggcag atcagttcgg gcatcagcgc gatgccgccg tcgcgctccg
     1381 tggcgcgcgc cacggccgtc atgttgtcga actccacgag gttcttgagt cgcgccaggt
     1441 cgatgtccgc gcgggcgagc cagtcgttcc agacggacgt gcgaaagctg tggtgcatga
     1501 tcaacgtctg gcggtcgaag gcctgcgcgc gcggtttgcc cttcatgctg gcggccacct
     1561 tcggcgatgc aaccggcacc agctgcaacg gcagcagccg tacggcctgt acgtccggtt
     1621 cgctggggac gtcgcctgcc aggatcaccg acaggtcgct gaagccgctg tgctgccccg
     1681 gccttgggtc cgtggtctgc acctgcaggt cgatgtccgg gtggctttgc tggaaatcgg
     1741 gcaggcgcgg cgcgaagagc tcgctggcga agaacggggg catcaccacc atcagctggc
     1801 gccgggcgtg gccaccgcgg gcgacgcgcg ccacggcggc ggccaccgcc tcgagcgcgg
     1861 gctcgaggtc atcgaggaac tgccggcctg ccgtcgtcag gacgaccgcc cgcgtacggc
     1921 gctggaacag gctgacgccg agctggtcct ccagttcctt gacctggtga ctgaccgcag
     1981 aaggcgtcaa tgccagccga tcggcggcca ccttgaagct cgagtggcgg gccgcgaggc
     2041 agaacgcgcg cagcaggcgc agcggcggtg ttttcaggga cgtggccatg gcattcatat
     2101 ccgtcacgcc acggtggcaa cttgcgtgcc acggcgggcg tgcagacaac tgctgtcgta
     2161 tcaggaactt gggacggcca aggtccgggc cgtcggaccg gttgcgccat tatagcgccg
     2221 cggtccagcg gggccgcacc gaacgcggtc accggcgcag ctcggacacg agccgcgcca
     2281 tcagcagcgc gtcgtggtcc tcgccgtcca cctgcaggta ctggcggcag cgcccctcct
     2341 cgacgaagcc gaagcgtcga tagagcgcga tggccgcgtg gttgtcggcc cggacgacca
     2401 gttccgcgcg ccggaggccg cgtgcgtccg cccccgccag cgtggccgcg agcatccgcg
     2461 tcccgacccc ccggccgcgg aattccggga cgacgcccat gccgaggacc gcgctgtggc
     2521 gcagcgtcgc ggcggccttc ggccgcaggt cgcaccagcc gaccaccgtc ccgtcgccgg
     2581 cgaccgcaac gtaatggatc gcgccgtccc gcaggctgtc cagcacgaac cgtcgagtcc
     2641 gcgagaacgg cggcgcctgc agcatcgcga ggtaacggcc ttcgcgcgcg atccggtcaa
     2701 gcgccgagtg aaagccggcg aggtgctccc tgccgaccgg cacgatccgg cagggcgcag
     2761 actcgtcgag gtccgacggc atggctacgg tggccatcgg cgcgatcgtc gcacgcgcgg
     2821 aacggtcgcg tcgtgggcct cctccctgcc atgctgtcgc ccaatctctt attctccgcg
     2881 gccacgcacc cggaccggtg cccctgccgc gggggaggca cggcgtccgg acagggcggt
     2941 cggccggccc gagccgtggg ccggatgtaa ctcaggaacg gggctgctgt ttcaacttca
     3001 tgctgtatct cgtcgatgcg agcgtcttca tcttccgcgc gtggcattcg gtgccggtgg
     3061 atgtttcgga ccgcgacggc aacccggtca acgccgtgtt cgggttcgcg cgcttcgtct
     3121 gcgaactgct cgagcgcgag tcaccgcgcc acattgccgt cgccttcgac gagagcctcg
     3181 aggcgggcta ccgcaacgag atctacccgg cctacaaggc caaccgcgac ccggccccca
     3241 tcgagctgca acggcagttc gcgttgtgtc gcgaaatctg cgcggcgctc ggactcgcgc
     3301 agtacggaag ctcgcggtac gaggccgacg acatcatcgg cacgctcgcg acccgggccc
     3361 gggtcgccgg cctccccgtc acgatcgtga gccgcgacaa ggacctcacc cagctgctgt
     3421 cgcccgtcga cgtgtactgg gacgcgatcg ccgacgtccg ttacgggtat gccgacatcg
     3481 aggcacgctt cggcgtgata cccgagcgca tggcggactt tctcgcgctg accggcgacg
     3541 cggtcgacaa cgtgccgggc gtgccgggca tcggccgcaa gacggcgcag aagctgctca
     3601 agcacttcga cacgctgcac ggcgtgttcg agaacctcga gcaagtgcgc aagctcaagt
     3661 tccgcaacgc cgtgttcgtg gccgacagtc tccggcaaca ccgcgacgcg gccttcctgt
     3721 cacgccggct gaccgcgatc gcctgcgaca tgccgttgcc gatttccagg gtcggtgacc
     3781 tgcgacggcg cgccccggac ctgtcgaccc tcgacacgtt ctgcgagacg cagggcttcg
     3841 gacgggtcct gcgcgaccag gcgcggcgca tctacgcgcg cctcggctga gggcgcgccg
     3901 gcttcgtcat ggacaaggag ccgtcggatg cggcgatcgg tgcgtggctc ggcgcggcga
     3961 ccggccgacg cttcgagtcg cggccggccg aaaccgtgca cggcggcagc atccatcact
     4021 gcgtgcgctg gccggcgcgg ggtggcgacg cgttcgtcaa gctgggtgcg gcggaggcga
     4081 tcgcgacgtt cgaagcggag gtcgacggcc tcgaggcgtt gcgggaggcg cgggcgttgc
     4141 gagtgcccga ggtgctcgcg gccggcgtcg cggccgggcg tgcggtcctg gcactcgagt
     4201 ggctcgacct gtcaccgcag gtcgcggagc gctcgagcgt gcaggcgcga ctcggcgagg
     4261 ctctcgctgc gcagcatcgc gtcacgggcc cgacctgggg ctggcaccgg gacaacacca
     4321 tcggcgccac accgcagccg aaccccggcg acgacgactg ggtgcgattc ttccgggagc
     4381 accgtctcgc g
//
LOCUS       DASPFR010000013         3276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1591392, whole genome shotgun sequence.
ACCESSION   DASPFR010000013 DASPFR010000000
VERSION     DASPFR010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591392"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..676
                     /gene="can"
                     /locus_tag="VD737_00295"
     CDS             11..676
                     /gene="can"
                     /locus_tag="VD737_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HXW09028.1"
                     /translation="MKKLPELFANNRRWASGTEARHPGFFAELAAQQAPRYLWIGCSD
                     SRVPANEIVGLAPGELFVHRNVANLVLHNDFSCLSVLQYAIEVLKVEHVMVVGHYGCG
                     GVAAAWEQRVLGLVDNWLQPIRDVAHLYRESLLQLATEEQRLDRLCELNVLFQARRVC
                     RTTIVQQAWQRGHSLTVHAWVYRLTDGLLRDLQFCATDLDSAEADFARVAGLGTIGAE
                     LAR"
     gene            complement(705..935)
                     /locus_tag="VD737_00300"
     CDS             complement(705..935)
                     /locus_tag="VD737_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09029.1"
                     /translation="MATRTKAKAKTSRRTTHRSSKGTKLYAVRAADGTFKDIQTYKRA
                     HGADVKRKSSAETAKAAAKKTTTRAKKASKKK"
     gene            1020..2300
                     /locus_tag="VD737_00305"
     CDS             1020..2300
                     /locus_tag="VD737_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXW09030.1"
                     /translation="MTPAQRLRNILGGSAGNFVEWFDWFAYSAFALYFSRAFLPEGDQ
                     TAQLLNAAAIFAVGFIARPVGAWLFGAYADRVGRRAALTLSVALMCGGSLMIALIPTG
                     FGVTSAALLLVARVLQGLSVGGEYGASATYLSEMAERQRRGLWSGFLYVTLTGGQLAA
                     LLLQVVLQRILTEQQLYDWGWRIPFAVGAGFAIVVWWIRRGIHETHAFTAVAGSARER
                     GRAWLLFRDYPRETLTVMALTAAGGVGFYTYTTYMQKLLVNSAGLDKPVVSQLMTGLL
                     AIFMVLPPLAGWLADRVGHRRTMLVSFGSLALVSVPVLNSLAQASDPRSAFLLALVPL
                     VLLSGYCGLSAIFKAELYPAHVRALGVAVPYAIAQAIFGGNAETTALYLKQVGHEPVF
                     FWLVAGLMAVGFVATLRLRETREHSLIHAELDSR"
     gene            complement(2350..>3276)
                     /locus_tag="VD737_00310"
     CDS             complement(2350..>3276)
                     /locus_tag="VD737_00310"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HXW09031.1"
                     /translation="QQANDADDGTNPRSGYWREVRESEPGYTAVRGQETNVLIQSGGQ
                     DWRELRNGPVTWIGAIMVLGVLAALIAYQLIKGGSKLEHRTGRMIPRWSVFDRVLHWY
                     VAVTFIVLAITGLSLLWGRHVLIPVIGKEAFAAWANLAKPIHDYLSLAFVAGLVVMLV
                     KFFHHSLPAKYDVVWLKQVGGYLDGSHPPAGFINAGEKVFYWTLVLAGTGLVISGFWL
                     LFPNLGFEREAMQTANIAHGITALILVTFVVLHIYLGTLGSEGAFEGMKSGYVDEGWA
                     HQHHSVWLEELKSGESVQPAAPARGTSVTAST"
BASE COUNT          454 a         1119 c         1115 g          588 t
ORIGIN      
        1 gggtccgctc gtgaaaaagc tgccagagct gttcgccaac aaccgtcgat gggcctcagg
       61 caccgaggcc cgccacccgg ggttcttcgc agagctcgcg gcgcagcagg caccgcggta
      121 cttgtggatc ggctgttcgg acagtcgcgt gcccgccaac gagatcgtcg gcctcgcgcc
      181 gggtgagctg ttcgtgcacc gcaacgtcgc gaacctcgtc ctgcacaacg acttcagctg
      241 cctgtcggtg ctgcagtacg cgatcgaggt gctcaaggtc gagcacgtca tggtggtcgg
      301 tcactacggc tgcggtggcg tcgcggcggc gtgggagcag cgcgtgctcg ggctcgtcga
      361 caactggctg cagccgatcc gcgacgtcgc ccacctctac cgggaatccc tgctgcagct
      421 cgcgacggag gagcagcggc tcgaccggct gtgtgaactc aacgtgctct tccaggcccg
      481 tcgcgtgtgc cgcacgacga tcgtgcagca ggcctggcag cgcgggcact cgctcaccgt
      541 gcacgcctgg gtctaccgcc tgaccgacgg gctgctgcgc gacctgcagt tctgcgcgac
      601 cgacctggac tccgcggagg cggatttcgc gcgggtcgcc gggctcggca cgatcggagc
      661 cgaactcgca cgctgacccg gccatgccga gccagtgcgt caggtcactt tttcttgctg
      721 gccttcttcg cgcgcgtcgt cgtctttttc gcggcggcct tcgcggtctc cgcgctggac
      781 ttccgcttca cgtccgcgcc gtgcgcgcgc ttgtacgtct ggatgtcctt gaacgtgccg
      841 tcggcggcac gcacggcgta gagcttggtg cccttggagc tgcgatgcgt cgtgcgccga
      901 gaggtcttgg ccttcgcctt ggtcctggtc gccatgagga tttcgccttt agtgatcggt
      961 ctccggcgcg acactagcgg cgagcaaacc gtgcagtcaa cgtacttcgg cgctcgccga
     1021 tgacaccggc ccagcgcctc cgcaacatcc tcggcggatc cgccggcaac ttcgtcgagt
     1081 ggttcgactg gtttgcgtac tcggccttcg cgctgtattt ctcgcgcgcc ttcttaccag
     1141 agggtgacca gacggcgcag ctgctcaacg cggcggcgat cttcgcggtg ggcttcatcg
     1201 cgcgcccggt gggcgcgtgg ctgttcggcg cgtacgccga cagggttgga cgacgcgccg
     1261 cgctcacgct gtcggttgcg ctgatgtgcg gcggctcgct gatgatcgcg ctgattccga
     1321 ccggattcgg cgtcacgtcg gcggcgttgc tgctcgtcgc ccgggtgctg cagggactct
     1381 ccgtcggcgg ggagtacggt gcctccgcaa cgtacctcag cgaaatggcc gagcggcagc
     1441 gccgcgggct ctggtcggga ttcctctacg tgacgcttac cggcgggcaa ctcgccgcgc
     1501 tcctgctgca ggtggtgctg cagcggatcc tcaccgagca acagctctac gactggggct
     1561 ggcggattcc cttcgcggtg ggcgccgggt tcgcgatcgt ggtctggtgg atccgtcgcg
     1621 ggatccacga gacgcatgcc ttcaccgcgg tcgcgggttc ggcgcgcgag cgcggacgag
     1681 cctggctcct gttccgggac tacccgcgtg agacgctcac cgtgatggcg ttgaccgctg
     1741 cgggcggggt cggcttctac acgtacacga cgtacatgca gaagctgctc gtcaacagcg
     1801 cgggcctgga caagcccgtg gtctcgcagc tgatgacggg cctgctcgcg atcttcatgg
     1861 tgctgccgcc gctggcgggc tggctcgcgg atcgcgtggg ccatcgccgg accatgctcg
     1921 tgtcgttcgg cagcctggcg ctcgtcagcg tgccggtcct caactcgctc gcgcaggcgt
     1981 cggacccacg gtcggcgttc ctgctggccc tggtcccact ggtgctgctc tcgggctact
     2041 gcggcctgag cgcgatcttc aaggccgaac tctatccggc ccacgtccgc gcgctgggcg
     2101 tcgcggtgcc gtatgctatc gcccaggcga tcttcggcgg caacgccgag accacggcgc
     2161 tgtacctcaa gcaggtcggc cacgagcccg tcttcttctg gctggtggcg gggctgatgg
     2221 cggtgggctt cgtggcgacg ctgcggctgc gcgagacgcg cgagcacagc ctgatccacg
     2281 ccgaactcga ctcccgctga aacgaaaagg ggcgccgcag cgcccctctt cgcacgcctg
     2341 gcgcggaggt caggtggagg cggtgacaga ggtcccgcgc gccggcgccg ccggctgcac
     2401 cgactcgccg ctcttcagtt cctcgagcca cacgctgtgg tgctgatgcg cccagccttc
     2461 gtccacgtac ccggacttca tcccctcgaa ggcgccctcg ctgccgagcg tgccgaggta
     2521 gatgtgcagg acgacgaacg tgaccaggat cagcgccgtg atgccgtgcg cgatgttcgc
     2581 cgtctgcatc gcctcgcgct cgaagcccag gttcgggaac agcagccaga agccagagat
     2641 cacgagtccg gtgccggcga gcacgagcgt ccagtagaac accttctcgc cggcgttgat
     2701 gaacccggcc ggcggatggc tgccgtcgag gtagccgccc acctgcttga gccacaccac
     2761 gtcgtacttg gccggcaggc tgtggtggaa gaacttgacc agcatcacga ccagccccgc
     2821 gacgaacgcg agcgagaggt agtcgtggat cggcttcgcg aggttggccc acgcggcgaa
     2881 ggcctccttg ccgatcacgg ggatcagcac gtggcggccc cagagcaggc tcaggccggt
     2941 gatggcgagc acgatgaacg tgacggccac gtaccagtgc agtacgcgat cgaacacgct
     3001 ccagcgcggg atcatgcgtc ccgtgcggtg ctcgagcttc gacccgccct tgatcaactg
     3061 gtaggcgatc agcgccgcga gcacgccgag caccatgatc gcgccgatcc acgtgaccgg
     3121 accgttgcgc agttcgcgcc agtcctggcc gccgctctgg atcaggacgt tggtctcctg
     3181 gccgcggacc gccgtgtatc ccggctccga ctcgcgcacc tcgcgccagt agcccgagcg
     3241 cggattggtc ccgtcatcgg cgtcattcgc ctgttg
//
LOCUS       DASPFR010000014         3174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1103338, whole genome shotgun sequence.
ACCESSION   DASPFR010000014 DASPFR010000000
VERSION     DASPFR010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103338"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2416)
                     /locus_tag="VD737_00315"
     CDS             complement(<1..2416)
                     /locus_tag="VD737_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXW09032.1"
                     /translation="MTPRPRWSFLAGALLPLLASAGTDPVKVTDLGEPVERQIADYQP
                     VHEWVQDPELLRTERGDRLEVQQVDGEELQTVKLRNFIPAIRFESGVAQIPRSSVDRL
                     AELLEQLRDRTNVRVHFVGHADSQPLSDELARVFDDNVGLSRERAGEVAEHFKNALNL
                     PPEAIAYDWAGDTRPLASNATPEGRSRNRRVEVEVWYDEPRRTVREQEVLVAEEIKRV
                     KVCRMETVCKLSYQEGHTRRARVRNLVLPLRYEDDSSSVSAEFTEQVRKAVANLSDKR
                     NVVVRFVGHSDDAPLSGRNARIYGDHLAMSKARARRVALEVQESLALPTAAIDSDGRG
                     AAYPVASNDTAQGRSLNRRVEVEFWYDDPLQQLPDEPQMCPGDGDEEMVTKVYDPPWG
                     RVEPLQLQNGRPVVPAGYAATLRRALGDVAGRTNPRLRFVGYTRNERLDRRTASVYGD
                     DVGLSTARARRAMDELRDDPALAGAPAEHEGRGYVQSDDVVNEGFVQGEDSFVRVQVV
                     YDEPAGRDDLEGVDVTRITRELTPKSPFELNLMRITVDGKPVDDPNRSSSDVQRCTDV
                     ALDEAQIQFRFDNLASRPRLGVAAHPNVVSVVDLVDGAIAAPVRFRMYTNYASFIERA
                     EVRVFRGGESLRSEPLAVAAVDASGLAEWQPDVEQLPGPAQELRFVLRAYDAKGNFDE
                     TSAQPLWIARETRPADEVSVMAVAEAGSVGGGDGLVADSAEASDEALEEPAPAAPVER
                     ELLATYGDSTLALHNIKLGSGTIKVQGSGVPLNHTVWVAGRPVPVDASGNFVAEEILP
                     DGAHTVE"
     gene            complement(2422..>3174)
                     /locus_tag="VD737_00320"
     CDS             complement(2422..>3174)
                     /locus_tag="VD737_00320"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HXW09033.1"
                     /translation="AVGAGVTEAEYVNRAQVVHELTGNAVSGEATATVRVMPDPTFDC
                     TDVTGKVFDDANRNGVQDAGERGIGGVRLSTTNGLLATTDAHGRYHITCAITPDEARG
                     SNFALKLDDRTLPSGFRMSTDALQVKRATRGKALKFNFGASIYRVVGLDLADAVFEPG
                     TDVMRIQWRPRVDRLLEELRKAPAVLRLSYVADLEDPALVDRRMQALRKQITDAWEAM
                     NCCYQLTVEPEVFWRLGAPVKQPVVRTQEGSQ"
BASE COUNT          420 a         1138 c         1105 g          511 t
ORIGIN      
        1 cttcgaccgt gtgggcgccg tcgggcagga tttcctcggc cacgaagttg ccgctggcgt
       61 cgaccggcac cggtcgtcca gcgacccaga cggtgtggtt cagcggcacg ccgctgccct
      121 ggaccttgat cgtgccgctg ccgagcttga tgttgtgcag tgcgagcgtg ctgtcaccgt
      181 aagtggccag cagttcgcgt tcgacaggcg ccgccggcgc gggctcctcc agcgcctcgt
      241 ccgaggcttc cgccgaatcg gcgaccaggc cgtcaccacc gccgacgctg ccggcttcgg
      301 ccacggccat gacggaaacc tcgtccgccg gccgcgtctc gcgcgcgatc cacaacggct
      361 gggccgaggt ctcgtcgaag ttgcccttcg cgtcgtacgc gcgcagcacg aagcgcaatt
      421 cctgcgcagg cccgggcaac tgctccacgt cgggctgcca ttccgccaga ccgctcgcgt
      481 cgaccgcggc gacggcgagc ggctcggacc gcagcgactc cccgccgcgg aacacccgga
      541 cctcggcgcg ctctatgaag ctcgcgtagt tcgtgtacat ccggaaccgc accggcgcgg
      601 cgatggcgcc gtcgaccagg tcgacgaccg acacgacgtt cgggtgcgcg gcgaccccga
      661 gacgggggcg ggacgcgagg ttgtcgaacc ggaactggat ctgcgcctcg tcgagcgcga
      721 cgtcggtgca ccgctgcacg tccgacgagc tgcggttcgg gtcgtcgacc ggcttgccgt
      781 cgaccgtgat gcgcatcagg ttcagctcga acgggctctt cggcgtcagc tcgcgcgtga
      841 tgcgcgtgac gtcgacccct tcgaggtcgt cgcgccccgc cggctcgtcg tagacgacct
      901 gcacgcgcac gaacgagtct tcgccctgca cgaagccctc gttgaccacg tcgtccgact
      961 gcacgtagcc acggccctcg tgttcggccg gcgcaccggc aagcgcaggg tcgtcccgca
     1021 gctcgtccat cgcgcgccgg gcgcgggcgg tcgacaggcc cacgtcatcg ccgtagacgc
     1081 tggccgtgcg gcgatcgagc cgctcgttgc gggtgtagcc gacgaaccgc aggcgcggat
     1141 tggtgcgacc ggcgacgtcg ccgagcgcgc gtcgcagcgt ggccgcatac ccggccggca
     1201 ccacgggcct gccgttctgc agttgcagcg gctcgacgcg gccccagggc gggtcgtaca
     1261 ccttcgtgac catctcctcg tccccgtcgc cggggcacat ctgcggctcg tccggcagtt
     1321 gctgcagcgg gtcgtcgtac cagaactcga cttcgacgcg cctgttcagc gaccgtccct
     1381 gcgccgtgtc gttcgaggcg accggatagg ccgcgccgcg accgtcgctg tcgatcgctg
     1441 ccgtcggcag ggcgagcgac tcctggacct ccagcgcgac gcggcgcgcg cgcgccttgg
     1501 acatcgcgag gtgatcgccg tagatccgcg cgttgcggcc ggacaggggc gcgtcgtcgc
     1561 tgtgaccgac gaagcgcacg accacgttgc gcttgtcgga gaggttggcg accgccttgc
     1621 gcacctgctc ggtgaactcg gccgacacgg acgagctgtc gtcctcgtac cgcaacggca
     1681 gcacgaggtt gcgcactcgg gcgcgacggg tgtgaccttc ctggtagctc agcttgcaga
     1741 cggtctccat gcggcagacc ttgacccgct tgatctcctc ggcgaccagc acctcctgct
     1801 cccgcaccgt ccggcgcggc tcgtcgtacc acacctcgac ttcgacgcgc cggttccgtg
     1861 accggccctc gggcgtggcg ttcgaggcga gcggccgcgt gtcgcccgcc cagtcgtagg
     1921 cgatcgcttc cggcggcagg ttcagggcgt tcttgaagtg ctcggccact tctcccgcgc
     1981 gctcccgcga gagcccgacg ttgtcgtcga acacccgcgc gagctcgtcg gagagcggct
     2041 gcgagtcggc gtgcccgacg aagtggacgc gcacgttcgt gcggtcgcgc agctgctcga
     2101 gcagctcggc gagcctgtcg acggagctgc gcggtatctg cgcgacgccc gattcgaagc
     2161 ggatcgcagg gatgaagttg cgcagcttca ctgtctgcag ctcttctccg tcgacctgct
     2221 gcacttcgag ccgatcgccg cgttccgtcc gcagcagttc cgggtcctgc acccactcgt
     2281 gcacgggctg gtagtcggcg atctggcgct cgaccggctc gccgaggtcg gtgaccttga
     2341 ccgggtcggt gcccgcagac gcgagcagtg gcagcagtgc gccggcgaga aacgaccatc
     2401 gagggcgcgg cgtcatggcg ttcactggct gccctcctgc gtgcggacga cgggctgctt
     2461 gacgggggca ccgagccgcc agaacacctc cggctcgacg gtgagctggt agcagcaatt
     2521 catcgcctcc caggcgtcgg tgatctgctt gcggagcgcc tgcatgcggc ggtcgacgag
     2581 cgccggatcc tcgaggtcgg ccacatacga caggcgcagg acggccggcg ccttgcgcag
     2641 ttcctcgagc agccggtcga cccgcggccg ccactggatt cgcatgacgt ccgtgcccgg
     2701 ctcgaagacc gcgtccgcga ggtcgaggcc cacgacccgg tagatcgacg cgccgaagtt
     2761 gaacttgagc gccttgccgc gcgtcgcgcg cttgacctgc agcgcgtcgg tcgacatgcg
     2821 gaagccgctc ggcagcgtgc ggtcgtcgag cttcagcgcg aagttgctgc cccgcgcctc
     2881 gtccggcgtg atcgcgcacg tgatgtggta gcgcccgtgc gcgtccgtgg tggcgagcag
     2941 gccgttggtc gtcgacagac gcacgccgcc gatgccgcgc tcgcccgcgt cctgcacgcc
     3001 gttgcggttg gcgtcgtcga acaccttgcc ggtcacgtcg gtgcagtcga aagtggggtc
     3061 cggcatcacg cggacggtgg cggtcgcctc gcccgagacg gcgttgccgg tcagctcgtg
     3121 cacgacctgg gcgcggttga cgtactcggc ctcggtcacg ccggcgccga ccgc
//
LOCUS       DASPFR010000015         2366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_127585, whole genome shotgun sequence.
ACCESSION   DASPFR010000015 DASPFR010000000
VERSION     DASPFR010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127585"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            496..1440
                     /gene="cysK"
                     /locus_tag="VD737_00325"
     CDS             496..1440
                     /gene="cysK"
                     /locus_tag="VD737_00325"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HXW09034.1"
                     /translation="MIYDNILGTIGRTPIVRINRLAPSHVTMYVKCEFFNPLSSVKDR
                     LAIAIIEDAEQSGQLKPGQTVVEATSGNTGIALAMVCAAKGYPFVALMVETFSVERRK
                     IMRMLGAKVILTPAAERGSGMVRRAEELAKKHGWFLARQFENEANPAYHRQTTGPEIL
                     SDFAGRRLDFFVTGWGTGGTLTGAGEMIRLARPEVRVIATEPAGASLLAGAEFKPHKI
                     QGWTPDFVPKVLNRAVAHEIVPVTDDEAIAASRALAAKEGIFCGISSGGTFAAAIKVA
                     EKAPQGAVLLAMLPDTGERYLSTPLFEGVPEGSDPDPG"
     gene            1572..>2366
                     /locus_tag="VD737_00330"
     CDS             1572..>2366
                     /locus_tag="VD737_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXW09035.1"
                     /translation="MPDSLRKVLIGTLIGACVVGFAWHIAGERAKRAPASSASPPSAA
                     GTAGRPGPSTGGGPTVPVVVRESRVERLLLQVEALGTARANESIDVTPKISNVVTAVR
                     FAEGQQVRKGAVLVELDAEQARADLAVAQAALTESTGQLKRSRELFETRVLSEQQIEQ
                     IEATHAANRARVASARARLNDTVIRAPFDGRAGLRRVSVGSLVESGTVITTLDDTSTI
                     KLDFTVPETVVASMKPGLELQATSVAYPGQAFAGRVASVDSRVDPQT"
BASE COUNT          379 a          833 c          816 g          338 t
ORIGIN      
        1 gcagcgtgat accgactcgg tagcttgagc gcgccttcgg tccggatccg catccagtcc
       61 gcaagagagc cagcaagacg catccatgcg ggcgcggcac gggccgtcca tggcccgtga
      121 cgctcttgcg gactggatcc gaacccgaac cgagcggcgc gccggagacg ctcgcgccag
      181 ctgcagcgcc tttcggaggg cgccgacgca tggcgtcgcg gcatggcggg ccgagaagag
      241 gttcgcgggg ccgtccgcgg cagggatgcc gcggcggagc cccagggatg ggttcacgcg
      301 tccccgcgaa cctgcttgtc gggccggcgt ggcgcgacgt gcgcggcccg tctgcgtgcc
      361 tgagacctca ggacgctgcg gcgacgaccg cgacatcgcg gttctgccgc cattgccacg
      421 cggcatgaac gcgccgcgcc agatggctat agtggcgcgc agaagtcgaa tgccacgcag
      481 gcaaaggacc gtcccatgat ctacgacaac atcctcggca ccatcggccg cacccccatc
      541 gtgcggatca accgcctcgc gccgtcgcac gtgacgatgt acgtcaagtg cgagttcttc
      601 aatccgctga gctcggtcaa ggaccgcctc gcgatcgcga tcatcgaaga tgccgagcag
      661 tccgggcagc tcaagcccgg gcagaccgtc gtcgaagcga cctccggcaa caccggcatc
      721 gcgctcgcca tggtctgcgc cgcgaaggga tatccgttcg tcgcgctgat ggtcgagacg
      781 ttctcggtcg agcggcggaa gatcatgcgc atgctcggcg ccaaggtgat cctgacgccc
      841 gcggccgagc gcggcagcgg catggtgcgc cgcgccgagg agctggcgaa aaagcacggc
      901 tggttcctcg cacggcagtt cgagaacgag gccaacccgg cgtatcaccg ccagaccacc
      961 ggccccgaga tcctgtcgga cttcgccggg aggcgactcg acttcttcgt caccgggtgg
     1021 ggcacgggcg gcacgctcac cggcgccggt gaaatgatcc ggctcgcccg tcccgaagtc
     1081 cgcgtgatcg cgaccgaacc cgccggcgcc tcgctgctgg ccggcgcgga attcaagccg
     1141 cacaagatcc agggctggac gcccgatttc gtcccgaagg tgctcaatcg cgccgtggcg
     1201 cacgagatcg tgccggtgac cgacgacgaa gcgatcgcgg cctcgcgcgc gctcgccgcg
     1261 aaggagggca tcttctgcgg catttcgtcg ggtggcacgt tcgccgccgc aatcaaggtg
     1321 gccgagaagg cgccgcaggg cgccgtgctg cttgcgatgc tgccggacac gggcgaacgc
     1381 tacctgtcca ctccgctgtt cgagggcgtg cccgagggct cggatcccga tccgggctga
     1441 acctgcggcg cgcatggcgg tccgagcctg cgcgtcctcg tcaccgactc cgcgatccaa
     1501 acgcgccgtt cgtgcgaacg gtgggcgata caatgccggt ttggcgacga cggtgaaggc
     1561 gacgacgatc catgcccgat tccctgcgta aggtgctgat cggcaccctg atcggcgcct
     1621 gcgtcgtcgg tttcgcatgg cacatcgccg gtgagcgcgc gaaacgtgcg ccggcctctt
     1681 ccgcgtcgcc accgtcggcc gccggcactg ccggtcgccc cggcccgtcc accggcggtg
     1741 gccccaccgt accggtcgtc gtccgcgaat cgcgggtgga gcgcctgctg ctgcaggtcg
     1801 aagcgctcgg caccgcccgc gccaacgaat cgatcgacgt gaccccgaag atctcgaacg
     1861 tcgtgacggc ggtgcggttc gccgaaggcc agcaagtgcg caagggcgcc gtgctcgtcg
     1921 agctcgacgc tgagcaggcc cgcgccgacc tcgcggtggc gcaggcggcg ctgaccgaaa
     1981 gcactgggca actcaagcgc agccgggaac tcttcgagac ccgggtgctg tccgagcagc
     2041 agatcgagca gatcgaagcg acgcacgcgg cgaaccgggc ccgcgtcgcc tcggcgcgtg
     2101 cgcgcctcaa cgacaccgtg atccgtgcgc cgttcgacgg ccgcgcaggc ctgcggcgcg
     2161 tcagcgtcgg cagcctggtg gagtctggca ccgtgatcac gacgctcgac gacaccagca
     2221 cgatcaagct cgacttcacc gtgcccgaga ccgtcgtcgc ttcgatgaag cccgggctcg
     2281 aactgcaggc gaccagcgtc gcgtatcccg gccaggcgtt cgcgggccgc gtcgcgagcg
     2341 tcgattcgcg tgtcgatccg cagacg
//
LOCUS       DASPFR010000016         6475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1592561, whole genome shotgun sequence.
ACCESSION   DASPFR010000016 DASPFR010000000
VERSION     DASPFR010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1592561"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1678
                     /locus_tag="VD737_00335"
     CDS             71..1678
                     /locus_tag="VD737_00335"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HXW09036.1"
                     /translation="MSVLETRIDRGGPEFVGNDATNRRLATELRELVARVREGGPAAA
                     REQHTARDKLLVRDRVDRLLDPGSPFLEIAPLAGHELYDDWIPSGGLVTGIGRVAGRE
                     CVIVANDATVKGGTYYPITVRKHLRAQDVALENALPCVYLVDSGGAFLPRQDDVFPDR
                     EHFGRIFYNQAQLSAKGIPQLAVVMGSCTAGGAYVPAMCDESIIVREQGTIFLGGPPL
                     VQAATGERVDAETLGGGDVHARQSGVVDYLADNDAHALWMARRLVGHLNRGRSQPPPE
                     PARAPRYPAEDLYGIVPRDTRHPYDVREVIARLVDASEFDEFKALYGTTLVCGFARIA
                     GYPVGIIANNGVLFSESALKGAHFVELCNRRRIPLLFLQNITGFMVGRKYEAGGIARD
                     GAKMVTAVACASVPKFTVIIGGSFGAGNYAMCGRAYGGRFLWTWPNSRISVMGGEQAA
                     TVLAIVKRDGMQRRGQEWPEDDELRFREEIRAQYETQGHPLYASARLWDDGVIDPVDT
                     RQVLALALAAATCREDEGEKPYGIFRM"
     gene            1816..3837
                     /locus_tag="VD737_00340"
     CDS             1816..3837
                     /locus_tag="VD737_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HXW09037.1"
                     /translation="MITSLLIANRGEIACRVIRTARRLGVRTVAVYSEADRQALHVRM
                     ADAAVCIGPAPARESYLDGERILQAAREHACEAIHPGYGFLSENAAFARACEAAGVIF
                     VGPPADAIDRMGSKSDARRLMAAAGVPVLPGYDDADQSDAALLGAARSLRFPLLIKPT
                     AGGGGKGMRIVRTADEFAEALAGARREAAKSFGDDRVLLERFVEKGRHVEIQVFADGH
                     GHAVHLHERDCSLQRRHQKVIEEAPAPGLAETTRAAMGEAAVAAALAVDYRGAGTVEF
                     LYDGRGFYFLEMNTRLQVEHPVTEMITGIDLVEWQLRVASGEPLPLAQHEVRREGHAV
                     EARLYAEDPERGFLPSTGRLTRLRLPAHLPHVRVDAGVEEGDEVSVHYDPMIAKLIAW
                     APDRTQALDGLRAALEATEVEGVRTNARFLWQILGHPAVREGDVSTRLLESDAALAGG
                     VDAQEFVDAWLVAAAARLCPLPDDAADDRHAAASPWRSTTGFRIAGPSVVRAAMRFDA
                     TFAPASLAAQSPQRQWLEFDQDGAGVTVIFDGRGHRVAAHRAPDGTLEARIDGRPVAA
                     RVEADRERTVVRRHCVRYDFVEDTGAEHRVSAEHEGHFRAPMPGHVLDVRVAVDQTVA
                     EGAVLMVLEAMKMEHSLTAPWAARVVEVKVRAGERVEEGSDLIRLEPAG"
     gene            3950..4582
                     /gene="ccmA"
                     /locus_tag="VD737_00345"
     CDS             3950..4582
                     /gene="ccmA"
                     /locus_tag="VD737_00345"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HXW09038.1"
                     /translation="MSFDGASFEARDINAWRGDRHILRDVSFRVDPGEFLKITGPNGV
                     GKTTLLRVICGLLPAETGELHWRGRSIRGARDEFNAELGYLGHLNSLKADLTARENLK
                     FLAGLRQSLTAADIDATLDRVGILSRGDLPARSLSAGQKRRLALARLLLADAALWILD
                     EPVTNLDTAGIQLVESVILEHVQGGGLAIAAAHQRLLDDAPCLRRLELEP"
     gene            4594..5265
                     /gene="ccmB"
                     /locus_tag="VD737_00350"
     CDS             4594..5265
                     /gene="ccmB"
                     /locus_tag="VD737_00350"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HXW09039.1"
                     /translation="MTEAARLVFVRDLTLAWRRWDEVAQPLIFYVVVTTMFPLATTPD
                     LSQLREIGGGVVWVAALLSSLLALEALFRADVEDGTMEQWVLSGQPLGWVLLAKVAAH
                     WVLTGLPLVIMSPIVGTGLGLPVGVWGVLMLSLLLGTGTLSVLGGIGAALTVGVRRGS
                     VLLSLLVLPLAMPMLIFGSRAVSMQMDGDSPAGPLQLLAAIFFMALSLGPIAMSAAIR
                     ISVEN"
     gene            5323..6054
                     /locus_tag="VD737_00355"
     CDS             5323..6054
                     /locus_tag="VD737_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HXW09040.1"
                     /translation="MSWTWFHKLASPPHAYRILGRIAPWFGWTALALILLATWWGLVK
                     APADYQQGDGFRILYVHAPSAWMSMFTYVVMAVAAGVGLVWRIKLAHGAAAACAPIGA
                     SFTFLALATGSIWGKPMWGTWWVWDARLTSELLLLFLYLGYMALRASFDDPQRADRAS
                     AVLALVGVINVPIIHYSVEWWTTLHQPASVTKLDKPSIQLAMLIPLFIMMVGFQLYFG
                     WAISRRLRGEILRRERNAKWLEEAA"
     gene            6229..>6475
                     /locus_tag="VD737_00360"
     CDS             6229..>6475
                     /locus_tag="VD737_00360"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HXW09041.1"
                     /translation="MTPRQKRMVTVVAILAGVGIATTFALKAFNQNLLYYYSPTQISA
                     GEAPTSRTFRVGGLVQNNSVKREAGSLEVTFVLTDFQK"
BASE COUNT          965 a         2188 c         2288 g         1034 t
ORIGIN      
        1 ctggggacag tccccgttca ttcaacgaga aagggggacc gtccccggtc gagaaaaagg
       61 gagagtccct ttgagcgtcc tcgaaacacg catcgatcgt ggcggcccgg agttcgtcgg
      121 caacgacgcg accaaccggc ggctcgccac ggagctgcgc gaactggtgg cccgggttcg
      181 tgaaggcggt ccggcggcgg cgcgcgagca acacaccgcc cgcgacaagc tgctggtgcg
      241 tgatcgcgtg gatcggttgc tcgacccggg ctccccgttt ctcgagatcg cgccactcgc
      301 ggggcacgaa ctctacgacg actggatccc gagcggcggc ctggtcactg ggatcgggcg
      361 ggtcgccggg cgcgagtgcg tgatcgtcgc caacgacgcg acggtcaagg gcggcaccta
      421 ttacccgatc acggtgcgca agcacttgcg tgcgcaggac gtcgcgctcg agaacgcgct
      481 gccgtgcgtg tacctcgtcg actccggcgg cgcgttcctg ccgcgccagg acgacgtgtt
      541 tccggaccgc gagcacttcg ggcggatctt ctacaaccag gcgcaactgt ccgcgaaggg
      601 catccctcag ctcgcggtcg tgatgggttc gtgcacggcc ggtggcgcgt acgtgcccgc
      661 gatgtgcgac gagtcgatca tcgtgcgcga gcagggcacg atcttcctcg gtggaccgcc
      721 gctcgtgcag gccgccacgg gcgagcgcgt ggacgcggag acgctcggcg gcggcgacgt
      781 ccacgcgcga cagtcaggcg tggtcgatta cctggccgac aacgacgcgc acgccttgtg
      841 gatggcgcgc aggctcgtcg gccacctgaa ccgcggtcgc tcgcagccgc cgccggaacc
      901 ggcccgcgcc ccgcgctacc cggccgagga tctctacggc atcgtgccgc gcgacacgcg
      961 acacccgtac gacgtgcgcg aagtcatcgc gcgcctcgtc gacgcgtcgg agttcgacga
     1021 gttcaaggcg ctgtacggca cgacgctcgt ctgcggcttc gcgcggatcg cgggctaccc
     1081 ggtcgggatc atcgccaaca acggcgtgct gttctcggag tcggcgctca agggcgcgca
     1141 cttcgtcgag ctctgcaacc ggcgccgcat cccgctgctg ttcctgcaga acatcaccgg
     1201 cttcatggtc ggccgcaagt acgaggcggg cgggatcgcc cgcgacggtg cgaagatggt
     1261 gaccgccgtc gcctgcgcgt cggttcccaa gttcacggtg atcatcgggg gctcgttcgg
     1321 cgccggcaat tacgcgatgt gcggccgggc atacggcggt cgcttcctct ggacctggcc
     1381 gaattcgcgc atctcggtga tgggcggcga acaggccgcc accgtgctcg cgatcgtcaa
     1441 acgagacggc atgcagcgcc gcggccagga gtggcccgaa gacgacgagc ttcggttccg
     1501 cgaggagata cgcgcgcagt acgaaaccca gggccacccg ctgtacgcga gcgcgcgcct
     1561 ctgggacgac ggcgtgatcg accccgtcga cacgcgacag gtgctcgcgc tcgcactcgc
     1621 cgcggccacc tgccgcgagg acgaggggga gaagccgtat ggaatcttcc gaatgtagtg
     1681 gtcagtggtc agtggctagt ggctagtggt caatgaccga gcacggcggt tcgggcgagc
     1741 gctcttacta gccactaacc actagtcact agccactggc tcttcagcca ctggatcctc
     1801 aatcgcggag acgcaatgat caccagcctc ctcatcgcca atcgcggcga gatcgcctgc
     1861 cgcgtcatcc gtactgcgcg gcggctgggc gtgcggacgg tcgcggtgta ctccgaggcc
     1921 gatcgtcagg ccctgcacgt gcgcatggcg gatgccgcgg tctgcatcgg cccggcgccg
     1981 gcgcgcgaga gctacctgga cggcgagcgg atcctgcagg ccgcgcgtga gcacgcctgt
     2041 gaggcgatac acccgggcta cgggttcctg tccgagaacg cggcgtttgc gcgcgcctgc
     2101 gaggcggccg gggtgatctt cgtcggaccg ccggccgacg cgatcgatcg catgggctcg
     2161 aagagcgacg cgcgccggct gatggccgcg gcgggggtgc cggtgctgcc gggctacgac
     2221 gatgcggacc agtcggacgc cgcgctgctc ggcgcggcac ggtcgctgcg ctttccgctg
     2281 ttgatcaagc cgacggccgg cggcggcggc aagggcatgc ggatcgtgcg caccgcggac
     2341 gaattcgccg aggcgctcgc gggcgcgcgt cgcgaggcgg cgaaatcgtt tggcgacgac
     2401 cgggtgctgc tcgaacgttt cgtcgagaag ggccgccacg tcgagatcca ggtgttcgcc
     2461 gacgggcacg gccatgcggt gcacctgcac gagcgcgact gctcgctgca gcggcgccac
     2521 cagaaggtca tcgaggaagc gccggcgccc gggctcgccg agacgacgcg cgcggccatg
     2581 ggcgaagccg ccgtggccgc ggcgctggcg gtggactacc gcggcgcggg cacggtcgag
     2641 ttcctctacg acggtcgcgg gttctatttc ctggaaatga acacgcgcct gcaggtcgag
     2701 cacccggtca ccgagatgat cacgggcatc gacctcgtcg agtggcaact gcgcgtggcg
     2761 tccggcgaac cgctgccgct cgcccagcac gaggtccgtc gcgagggcca cgccgtggag
     2821 gcgcgcctgt atgccgagga cccggagcgc ggcttcctgc cctcgaccgg acgtctcacg
     2881 cgcctgcgcc tgccggcgca cctgccgcac gtgcgtgtcg acgctggcgt cgaggagggc
     2941 gacgaggtca gcgttcacta cgacccgatg atcgcgaagc tcatcgcctg ggcccctgat
     3001 cgcacgcagg cgctcgatgg cctgcgcgcg gcgctcgagg cgacggaggt ggagggcgtg
     3061 cgcaccaacg cgcgcttcct ctggcagatc ctcggccacc cggccgtgcg cgagggtgac
     3121 gtgagcacgc gcctgctcga gtcggacgcc gcgctcgcag ggggtgtgga cgcgcaggaa
     3181 ttcgtcgacg cgtggctggt cgccgcggcg gcccggctct gcccgctgcc agacgacgcg
     3241 gccgacgacc ggcacgctgc ggcgagcccg tggcgatcga cgaccggctt ccgcatcgcc
     3301 ggtccgtccg tcgtgcgtgc cgccatgcgc ttcgatgcga cgttcgcgcc agcctcgctg
     3361 gccgcgcaat caccgcagcg ccagtggctg gagttcgacc aggacggcgc cggtgtcaca
     3421 gtgatcttcg atggtcgggg tcaccgggtc gctgcgcatc gcgcgccgga cggtacgctc
     3481 gaagcgcgca tcgacgggcg tccggtcgcc gcgcgcgtag aggccgatcg cgaacgcacc
     3541 gtcgtccggc gccactgcgt gcgctacgac ttcgtcgagg acaccggcgc cgagcaccgg
     3601 gtctctgcgg agcacgaagg gcacttccgc gcaccgatgc ctggacacgt gctggacgtg
     3661 cgcgtcgccg tcgaccagac cgtcgccgag ggtgccgtgc tgatggtgct cgaggcgatg
     3721 aagatggagc actcgctgac cgcgccctgg gccgcccggg tcgtcgaagt caaggtccgg
     3781 gcgggcgagc gagtcgagga aggcagcgac ctgatccgtc tcgaacccgc agggtgactg
     3841 cgggaagtcc ggacattgga atgagtctgt tttgctagac ttcgcgccca atccaccgcc
     3901 tcgcgcgacg tgcaggcgga catgaaagag cgaatcgcgc ggaggcggga tgagtttcga
     3961 cggggcctct ttcgaggccc gcgacatcaa cgcgtggcgg ggtgacaggc acatcctgcg
     4021 cgacgtgtcc ttccgcgtcg atccgggcga attcctgaag atcaccgggc ccaacggcgt
     4081 cggcaagacg acgctgctgc gcgtgatctg cgggctcctg ccggccgaga cgggcgaact
     4141 gcactggcgg ggcaggtcga tccgcggcgc gcgcgacgaa ttcaacgccg aactcggata
     4201 cctcggccac ctcaattcgc tgaaggcgga cctgacggcg cgcgagaacc tcaagttcct
     4261 cgccggattg cgccagtcgc tgacggctgc ggacatcgac gcgacgctcg accgggtcgg
     4321 catcctgtcg cgcggcgacc tgccggcgcg cagcctttcg gcgggacaga agcgccggct
     4381 cgcgctcgcg cgcctgctgc tggccgatgc ggcgctgtgg atcctcgacg agccggtcac
     4441 caacctcgac acggccggca tccagctggt cgagtcagtg atcctggaac acgtgcaggg
     4501 tggcgggctc gcgatcgccg cggcacacca gcgcctgctc gacgacgcac cctgcctgag
     4561 gcgactggag ctcgagccgt gacccgcacc ccgatcaccg aggcggcccg gctcgtcttc
     4621 gtccgcgacc tcacgctcgc ctggcggcgc tgggacgagg tcgctcagcc gctgatcttc
     4681 tacgtcgtcg tcacgacgat gttcccgctc gccacgacgc cggacctctc gcagctgcgc
     4741 gagatcggcg gcggcgtcgt gtgggtcgcg gccctgctgt cgtcgctgct cgcgctcgag
     4801 gcgctgttcc gcgccgacgt cgaggacggc acgatggagc agtgggtgct gtccggccag
     4861 ccgctcggct gggtattgct tgccaaagtg gcggcacatt gggtgctgac tgggctgcca
     4921 ctcgtcatca tgtcgcccat cgtcggcacc ggcctcggcc tgccagtggg tgtctggggc
     4981 gtgctgatgc tgtcgctgct gctcggcacg ggtacgctca gcgtgctggg cggcatcggc
     5041 gcggcgctca cggtcggcgt gcggcgcggc agcgtgctgc tgtcgctgct ggtgctgccg
     5101 ctcgcgatgc cgatgctgat cttcggctca cgggcggtgt ccatgcagat ggacggcgac
     5161 tcgccggccg gccctctgca gctgcttgcc gcgatctttt tcatggcgct gagcctcggg
     5221 ccgatcgcca tgtctgcggc gatccgcatc agcgtcgaga attgacacgt atttaacgca
     5281 acgactgaac gcaacgagcg ttgcagggtc aagagggaca gcatgtcgtg gacctggttt
     5341 cataagctcg cttcgccccc gcatgcctac cgcatcctcg ggcggatcgc cccgtggttc
     5401 ggctggacgg cgctggcgct gatcctgctc gccacctggt ggggcctggt caaggccccg
     5461 gccgactacc agcagggcga cgggttccgc atcctgtacg tccacgcgcc gagcgcctgg
     5521 atgtcgatgt tcacttacgt cgtgatggcc gtggcggccg gcgtcggcct ggtctggcga
     5581 atcaagctcg cgcacggcgc ggcggcggcc tgtgcgccga tcggcgcgtc gttcacgttc
     5641 ctcgcgctcg ccaccggctc gatctggggc aagccgatgt ggggcacctg gtgggtctgg
     5701 gatgcgcggc tcacgtcgga gctgctgctg ctgttcctct acctcggcta catggccctg
     5761 cgcgcctcgt tcgacgaccc gcagcgcgcc gaccgggcga gcgcggtgct cgcgctggtc
     5821 ggcgtcatca acgtgccgat catccactac tcggtcgagt ggtggactac gctgcaccag
     5881 cccgcgagcg tgacgaagct cgacaagccg tcgatccagc tggccatgct gattccgctg
     5941 ttcatcatga tggtcggttt ccagctctac ttcggctggg cgatctcgcg tcgcctgcgc
     6001 ggtgaaatcc tccgccgcga acgcaatgcc aagtggttgg aagaagcagc ttaatatcat
     6061 ggacttacaa aactttcttg agatgggcgg ttacgcgaaa ttcgtgtggc cggcctacgg
     6121 cctgaccgcg ctggttctcg tctggaactg gtggtcggca agacgcagtg aaacggaagc
     6181 ccagtccgcc gcgcgtcggc ggaacgaact tgcctcggag aaccgaacat gaccccgcga
     6241 cagaagcgca tggtgaccgt ggtggccatc ctggccggcg tcggcatcgc cacgacgttc
     6301 gccctcaagg ccttcaacca gaacctgctc tactactaca gcccgaccca gatcagtgcg
     6361 ggcgaggcgc cgacgtcgcg cacgttccgg gtcggcggcc tggtgcagaa caacagcgtg
     6421 aagcgcgagg cgggcagcct cgaggtcacc ttcgtgctga ccgacttcca gaagg
//
LOCUS       DASPFR010000017         2849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_249915, whole genome shotgun sequence.
ACCESSION   DASPFR010000017 DASPFR010000000
VERSION     DASPFR010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249915"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VD737_00365"
     CDS             complement(<1..373)
                     /locus_tag="VD737_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09042.1"
                     /translation="MAPTETAIHRRVAACRTGHEATLIARLGAGWAVLGDPQVLPGYC
                     LLLPDPVVPHLNVMPPSGQQAFLADMARLGDAVLRVTGAVRVNYAMFGNVEPALHAHV
                     FPRYADEPASLVTAHPWTYDWG"
     gene            complement(377..718)
                     /locus_tag="VD737_00370"
     CDS             complement(377..718)
                     /locus_tag="VD737_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012277143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HXW09043.1"
                     /translation="MRPVPTAILLLAASNVFMTFAWYAHLKNLSGRAWYVAALVSWGI
                     ALFEYLLQVPANRIGHTALTLPQLKILQEVISLSVFVPFVLFYMRQPIKLDYLWASLC
                     MLGAVYFMFRR"
     gene            complement(737..1354)
                     /locus_tag="VD737_00375"
     CDS             complement(737..1354)
                     /locus_tag="VD737_00375"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HXW09044.1"
                     /translation="MIRDVVFDVGGVLLELRYEPFIRYLADAGIDMHDLPDWLTKVDL
                     AGHERGELAGEELLHRIASMASRPLDLADLRSRWLDMFERSEEMFTLAAGLMQEYRVY
                     LLSNAGDLHWQHFDRLYGLDSLVHGACASFRVRAIKPSADIYRKAEAMFGLDPSTTVF
                     IDDLPQNVVGARACGWNAIHHNRPHATRLQLRALGVRLPPPFDRE"
     gene            complement(1351..2568)
                     /locus_tag="VD737_00380"
     CDS             complement(1351..2568)
                     /locus_tag="VD737_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09045.1"
                     /translation="MNAAGGWQPLGVPFEILLFALTLLGVALLHGRTLEVALTGLATV
                     TSYKLLTAGFEEGPGVGGLLTHLQGEWVVLANLFGLLMGFALLSRHFEASHIPRRLPD
                     YLPDDWRGGFVLLVMVFVLSSFLDNIAAAIIGGTVAGTVFRRRLHIGYIAGIVAASNA
                     GGSGSVVGDTTTTMMWIDGVSPLEVLHAYVAAGVALVVCGIPASLQQQRHSPILKHGL
                     ASERLDGARVAIVALILAAVIVVNVTTNVALPEAAGTFPFLGFAVWSVLLLTAGWRRP
                     DWSVLPSTLRGTVFLLSLVLTASMMPVERLPDASWPTTLGLGFLSAVFDNIPLTKLAL
                     EQGGYDWGMLAYAVGFGGSMLWFGSSAGVAITNLYPQARSVGAWLRGGWHVALAYVTG
                     FTALMLAMGWRPA"
     gene            complement(2565..>2849)
                     /locus_tag="VD737_00385"
     CDS             complement(2565..>2849)
                     /locus_tag="VD737_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09046.1"
                     /translation="MGHEARERCAEDDLCAEVLLDLFAGRTPPTTAQVRARLRDAPAA
                     RKFFDPACDWAPEGDFDRCTEVDRFGFVLRLRQAPDGLRDLERLDVPAAA"
BASE COUNT          513 a         1000 c          974 g          362 t
ORIGIN      
        1 cgccccagtc ataggtccag ggatgcgccg tgacgagcga tgccggttcg tcggcgtagc
       61 gcgggaacac gtgcgcgtgc agggccggct cgacgttgcc gaacatcgcg tagttgacgc
      121 gcaccgcgcc cgtcacccgc agcaccgcgt cgccgagccg cgccatgtcg gcgagaaaag
      181 cctgctggcc ggacggcggc atcacgttca ggtgcggcac gaccggatcg ggcaggagca
      241 ggcagtagcc gggcagcacc tgcgggtcgc cgagcaccgc ccatcccgcc cccaggcgcg
      301 cgatcagcgt cgcctcgtgg ccggtcctgc aagccgcaac gcgacggtgg atcgccgtct
      361 cggtgggggc cactcgtcac ctccggaaca tgaaatacac cgccccgagc atgcagagcg
      421 aggcccagag gtagtcgagc ttgatcggct ggcgcatgta gaacagcacg aacggcacga
      481 acacggaaag cgagatgacc tcctgcagga tcttgagctg cggcaacgtc aacgccgtgt
      541 gtccgatgcg gttcgccggc acctgcagca ggtattcgaa cagcgcgatg ccccagctca
      601 cgagcgcggc gacgtaccac gcgcggccgc tcaggttctt gaggtgcgcg taccacgcaa
      661 acgtcatgaa gacgttcgac gcggccagca gcaggatggc ggtaggcacg ggcctcatct
      721 cgaacggcac ggcgcgtcac tcccggtcga acggcggcgg cagacggacg ccgagggcgc
      781 gcaactgcag ccgcgtggca tgcgggcggt tgtggtgaat cgcgttccag ccgcaggcgc
      841 gcgcgcccac gacgttctgc ggcaggtcgt cgatgaagac ggtcgtggac gggtcgaggc
      901 cgaacatcgc ctccgccttg cgatagatgt ccgcgctggg cttgatcgcc ctgacgcgga
      961 acgaggcgca ggcgccgtgc acgagggagt cgagcccgta gaggcgatcg aagtgctgcc
     1021 agtgcaggtc gcccgcgttc gacagcaggt agacgcggta ctcctgcatg aggccggccg
     1081 cgagcgtaaa catctcctcg gaccgttcga acatgtcgag ccatcgcgaa cgcaggtccg
     1141 cgaggtcgag cggccggctc gccatcgacg cgatgcgatg caggagttcc tcgcccgcca
     1201 gttccccgcg ctcgtggccc gcgaggtcga ccttcgtgag ccagtccggc aggtcgtgca
     1261 tgtcgatgcc ggcgtccgcc agatagcgga tgaacggctc gtagcgcagt tcgagcagca
     1321 cgccgccgac gtcgaagact acgtcgcgga tcatgccggg cgccatccca tcgcgagcat
     1381 gagcgccgta aagcccgtga cataggccag cgcgacgtgc catccgccgc gcagccacgc
     1441 gccgacggac cgagcctgcg gatacaggtt cgtgatcgcc acgcccgccg aggagccgaa
     1501 ccagagcatg gaaccgccga agcccacggc gtaggccagc atgccccaat cgtagccgcc
     1561 ctgctcgagc gcgagcttgg tgagcggaat gttgtcgaag accgcggaga ggaagccgag
     1621 ccccagcgtc gtcggccacg acgcgtccgg cagacgttcg accggcatca tcgaggccgt
     1681 cagcaccagg ctcagcagga agaccgtgcc gcgcaacgtc gagggcagta cggaccagtc
     1741 cggccggcgc cagccggcgg tcagcagcag caccgaccac acggcgaagc cgaggaacgg
     1801 aaaagtgccg gcggcctccg gcaacgccac gttggtcgtc acgttgacga cgatcaccgc
     1861 ggccaggatc agcgccacga tcgccacccg cgcgccgtcg agacgttcgc tcgcgagccc
     1921 gtgcttgagg atcggcgagt ggcgctgctg ctgcagcgaa gccgggatac cgcagaccac
     1981 gagcgcgacg ccggcggcca cgtatgcgtg cagcacctcg agcgggctca cgccgtcgat
     2041 ccacatcatc gtcgtggtcg tatcgcccac gacgctgccc gagcccccgg cattggacgc
     2101 cgcgacgatt ccggcgatgt acccgatgtg caggcgccgg cgaaacaccg tgccggccac
     2161 cgtgccgccg atgatcgccg cggcgatgtt gtcgaggaag ctcgacagca cgaacaccat
     2221 cacgagcagc acgaagccgc cgcgccagtc gtcgggaagg tagtcgggga gccggcgcgg
     2281 gatgtggctg gcttcgaaat gccgcgacag cagggcaaat cccatcagca gcccgaacag
     2341 gttggcaagg accacccact ccccctgcag gtgcgtgagc agccctccga cgcccgggcc
     2401 ttcttcgaac ccggccgtca gcagcttgta cgacgtcacc gtcgcgagcc cggtcagcgc
     2461 cacttcgagc gtgcggccgt gcagcagcgc gactccgagc agcgtcagcg cgaacagcag
     2521 gatctcgaac ggaacaccga gcggctgcca gcccccggcg gcgttcatgc ggccgccggc
     2581 acgtcgaggc gctcgaggtc gcgcagcccg tcgggcgcct ggcgcagccg cagcacgaac
     2641 ccgaagcgat cgacttccgt gcagcggtcg aaatccccct ccggcgccca gtcgcaggcc
     2701 ggatcgaaga acttccgcgc cgccggcgcg tcgcgcaatc gcgcgcggac ctgcgccgtc
     2761 gtcggtggcg tgcggccggc aaacaggtcc agcaggacct ccgcgcacag gtcgtcctcc
     2821 gcgcaacgct cgcgcgcctc gtgacccat
//
LOCUS       DASPFR010000018         3822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1348772, whole genome shotgun sequence.
ACCESSION   DASPFR010000018 DASPFR010000000
VERSION     DASPFR010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348772"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..944)
                     /locus_tag="VD737_00390"
     CDS             complement(90..944)
                     /locus_tag="VD737_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HXW09047.1"
                     /translation="MSPARPSMHPTLTRTLRLLGLLEAGRRLELQRRLRDQRALPDAM
                     IIGAMKSGTSSLHNYLTQHPGVIAPLRKEVHYFDVHFDQGERWYRANFGRLGEPGLNL
                     DSSPYYLSHPLAPQRAHALVPQARLIVLLRDPVRRAYSHYWHERDKKRETLSFEEAIA
                     AEPVRLGDDEQRLALGEIRHSRAHQHFGYLSRGRYAEQLERWWQFYPREQTLVLRFED
                     LACAPMDVYWRTLDFLGLPRVERVRLEARNARKYPPMSAATAERLRDYFAPHEARLQA
                     LLGDGVRA"
     gene            complement(941..2356)
                     /gene="lpdA"
                     /locus_tag="VD737_00395"
     CDS             complement(941..2356)
                     /gene="lpdA"
                     /locus_tag="VD737_00395"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXW09048.1"
                     /translation="MVVIGAGPGGYTAAFRAADLGLKVALVERWPTLGGVCLNVGCIP
                     SKALLHAAKVIDEAQAMSAHGVEFGPPRIDAGKLRAWKDQVVGRLTGGLTALAKQRKV
                     TVLRGVAVFDGDHAVRVTPEEGESSVVSFAHCIVAAGSESLRLPGLPDDPRVIDSTGA
                     LDLDLPKRLLVVGGGIIGLEMATVYAALGVKVSVVELTEGLMPGCDRDLVRPLEKRIA
                     KQYESILLRTKVTKIEALDDGLRVTFEGDKAPAPQLYDKVLVAVGRTPNGKGLECEKA
                     GVAVDGRGFVPVDRQQRTNVSHIFAIGDIVGPPMLAHKATHEAKVAAEVIAGHKRHFD
                     ARSIPSVAYTDPEIAWTGLTETDARAKGIPYEKAVFPWAASGRSLAIGRDEGFTKLLF
                     DPETHRILGGGIVGTNAGDLISEVMLAIEMGADATDVGLTIHPHPTLSETVAMAAEAF
                     DGTLTDLYLPRKAARKQHEAG"
     gene            complement(2591..2842)
                     /locus_tag="VD737_00400"
                     /pseudo
     CDS             complement(2591..2842)
                     /locus_tag="VD737_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
     gene            complement(2839..>3822)
                     /locus_tag="VD737_00405"
     CDS             complement(2839..>3822)
                     /locus_tag="VD737_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HXW09049.1"
                     /translation="GTRPSHQPPPAAGEGARPIDEPGFSRAHASPSIRKLARELGVDL
                     ARVRGTGTKERITDADVKGWVKQALASRAAPAAGGGALPAIPTVDFAAFGPVERKPLS
                     RIQKIAGPRLHASWVNLPHVTQFDEADITEMEQARSNLKGEATQRGIKLTPLAFVMRA
                     CVQALKEFPQFCASLDAAAGELVYKKYVHLGFAADTPNGLVVPVVRDADRKDVFEIAR
                     ELAELSEKARAGKLTAAEMQGACFTISSLGGIGGTAFTPIINAPEVAILGVSRSTHKP
                     VYRDGGFVPRLMLPLSLSYDHRVIDGAAAVRFTSFLAAALADVDGLLRAIP"
BASE COUNT          535 a         1345 c         1327 g          615 t
ORIGIN      
        1 tcagcacttc tgcataccgt ctccgggtgt acctgcggcg atgcacctcc tgcgcgacgg
       61 cagctggcgc gccggctacg aagatgctct tacgcgcgga cgccgtcgcc gagcagcgcc
      121 tgcagccgcg cctcgtgcgg cgcgaaatag tcgcgcagtc gctccgccgt cgccgcactc
      181 atcggcggat acttgcgggc gttgcgagct tcgagccgca cgcgctcgac gcggggcagc
      241 ccgaggaagt cgagcgtccg ccagtacacg tccatcggcg cgcaggcgag gtcctcgaag
      301 cgcagcacca gcgtctgctc gcgcggatag aactgccacc agcgctcgag ctgctcggcg
      361 taacgcccgc gcgagagata gccgaagtgc tggtgggcgc ggctgtgccg gatctcgccg
      421 agcgcgagtc gctgctcgtc gtcgccgagc cgcaccggtt ccgcggcgat cgcctcctcg
      481 aacgacagcg tctcgcgctt cttgtcgcgc tcgtgccagt agtgggagta cgcacgacgc
      541 acgggatcac gcagcagcac gatcagccgc gcctgcggca cgagcgcgtg cgcgcgctgc
      601 ggcgcaagcg ggtgcgagag gtaatacggg ctgctgtcga ggttgagccc cggctcgccg
      661 agccggccga agttcgcgcg ataccagcgc tcgccctggt cgaaatgcac gtcgaagtag
      721 tgcacctcct tgcgcagcgg cgcgatcacg cccgggtgct gcgtcaggta gttgtgcaac
      781 gagctggtgc cggacttcat ggcgccgatg atcatggcgt ccggcagcgc ccgctggtcg
      841 cgcagccggc gctgcagttc gaggcgtcgc ccggcctcga gcagcccgag cagtcgcaac
      901 gtcctcgtga gcgtcgggtg catgctcggc cgcgcggggc tcaaccggcc tcgtgctgct
      961 tccgcgcggc cttccttggc aggtacaggt cggtcagcgt gccgtcgaag gcttccgccg
     1021 ccatggccac cgtctcggac agcgtggggt gcggatgtat cgtgaggccg acgtccgtgg
     1081 cgtcggcgcc catctcgatc gccagcatga cttcggaaat caggtcgccg gcgttggtgc
     1141 ccacgatccc gccgccgagg atccggtgcg tttccggatc gaacaacagc ttcgtgaaac
     1201 cttcgtcgcg cccgatcgcc agggaacgcc cgctggccgc ccacgggaac acggctttct
     1261 cgtatggaat gcccttggcc ctggcgtcgg tctcggtgag gccggtccag gcgatctcgg
     1321 gatcggtgta cgccacggac gggatcgagc gcgcatcgaa gtgtcgcttg tgcccggcga
     1381 tgacttcggc ggcgaccttg gcctcgtgcg tcgccttgtg ggcgagcatc ggcggcccga
     1441 cgatgtcgcc gatcgcgaag atgtgggaga cgttggtgcg ctgctgcctg tccaccggaa
     1501 cgaagccacg gccgtcgacg gcgacgccgg ccttctcgca ctcgaggccc ttgccgttcg
     1561 gcgtgcgtcc cacggccacg aggaccttgt cgtagagttg cggcgcgggc gccttgtcgc
     1621 cctcgaacgt gacgcgcagc ccgtcgtcga gcgcctcgat cttcgtgacc ttggtgcgca
     1681 gcaggatcga ctcgtactgc tttgcgatgc gtttctcgag cggacgcacc aggtcccggt
     1741 cgcagccggg catcagaccc tcggtcaatt cgacgacgct gaccttgacg ccgagcgccg
     1801 cgtacacggt cgccatctcg aggccgatga tgccgccgcc cacgacgagc agccgcttcg
     1861 gcaggtcgag gtccagcgcg ccggtcgagt cgatcacgcg cggatcgtcg ggaagccccg
     1921 gcagccgcag cgactccgag ccggcggcaa cgatgcagtg ggcgaagctc acgacgctcg
     1981 actcgccctc ctccggggtc actcgcacgg cgtgatcgcc gtcgaacacg gcgacgccgc
     2041 gcagcaccgt gaccttgcgt tgcttcgcga gcgcagtgag gccgcccgtc agccggccca
     2101 cgacctggtc cttccacgcc cggagcttgc cggcatcgat cctgggcggc ccgaactcca
     2161 cgccatgggc ggacatcgcc tgcgcctcgt cgatcacttt cgcagcgtga agcagcgctt
     2221 tcgaaggaat gcagccgacg ttgaggcaga cgccgcccag ggtgggccaa cgttccacga
     2281 gcgcgacctt gagcccgagg tcggcggcgc ggaacgcggc ggtgtagcca cccgggcccg
     2341 cgccgatcac gaccacgtcg aagtcatggc gtgccgccgt cccagcctcg gacgatccag
     2401 ttgcctgctg cggtgcctgc gcgccggtcg acgcgggcgc ggcctcgcgc gcgagatcct
     2461 ccggcgacag cggtttgatc ggcacggtcg agacgtaggg ctcaccgccg aacggctcgg
     2521 cctggcgcgg gccgggtgtc gacggcagct cttcggcctt cagctgcggt gcgccggcga
     2581 gttccatgat cttcgtgccg gtcgcgacct tgtcgcccgg tttgacgagc agcgctgcga
     2641 cgacaccggg tgcgggcgac gggacgtcca tcgacgcctt gtcgctttcg agcgtgacga
     2701 gcggcgtgtc gcgctcgacc gcgtcgccca ccttgaccag ccactccacg accgagactt
     2761 cggcctgcgt gccgaggtcc ggcagccgga tctcgcgcga gggcgtgctc ggtgacttcg
     2821 ccccctctcc ttcggtgctc acgggatcgc cctcagcagt ccgtccacgt cggcgagtgc
     2881 ggcggccagg aacgacgtga atcggacggc cgccgcgccg tcgatgacgc gatgatcgta
     2941 cgacagcgac agtggcagca tcagccgcgg cacgaatccg ccgtcccggt acaccggctt
     3001 gtgcgtcgag cgcgacacgc cgaggatcgc gacttccggc gcgttgatga tcggcgtgaa
     3061 cgcggtgccg ccgatgccgc ccaggctcga gatcgtgaag caggcgccct gcatctccgc
     3121 ggcggtgagc ttgccggccc gcgccttctc gctgagctcg gccagttcgc gcgcgatctc
     3181 gaacacgtcc ttgcggtcgg cgtcgcgcac caccggcacg acgagcccgt tcggcgtgtc
     3241 ggcggcgaaa cccaggtgca cgtatttctt gtagacgagt tcgcccgccg cggcgtccag
     3301 cgacgcgcag aactgcggga attccttgag cgcttgcacg caggcgcgca tcacgaacgc
     3361 gagcggagtg agcttgatgc cgcgctgggt ggcctcgccc ttgaggttcg aacgtgcctg
     3421 ctccatctcc gtgatgtcgg cctcatcgaa ctgcgtgacg tgcggcaggt tgacccagct
     3481 ggcgtgcagc cgagggccgg cgatcttctg gatgcgcgac agcggcttgc gctcgaccgg
     3541 cccgaacgcc gcgaaatcca ccgtgggaat cgccggcagt gcgccgccgc ccgcggccgg
     3601 tgcagccctg gacgccagcg cctgcttgac ccagcccttg acgtcggcgt cggtgatgcg
     3661 ttccttggtg cccgtgcccc ggacgcgcgc gaggtcgacg ccaagctcgc gcgcaagctt
     3721 gcggatcgat gggctggcgt gcgctcggga gaagcccggc tcgtcgatcg gcctcgcgcc
     3781 ctcgcccgcc gccggaggag gctggtgcga cggtctcgtt cc
//
LOCUS       DASPFR010000019         8369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1227506, whole genome shotgun sequence.
ACCESSION   DASPFR010000019 DASPFR010000000
VERSION     DASPFR010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8369
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227506"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VD737_00410"
     CDS             <1..1023
                     /locus_tag="VD737_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXW09050.1"
                     /translation="WQPMGETFAIRASYSESFTAPTLYELFGPTSQGFTSSLSLNQYD
                     SSGNSLGTTDTPAQYRSQSGSNTQLDPSESTNYSVGFEWNPEGAMQGLQIGVDYWSIE
                     EEDIVDVLPSSIVLQSVEALGPASPYASYVRRGVSSAGEVYFGTGTPISAPGEITGSV
                     GDTVWLSNSLINLSSIEQDGLDVRIGYSFETETAGTFSAQFISTFLFNYDSLPVPTEP
                     LIELSGTYHDDYGLFPDYRAFLQLGWAMGGFTLGINGQYLPEGDDVTSQEPPYVKFDD
                     YMSWDIRAGYDFTEQGFPLSFNAGVNNVFDEEPVFIESEGNQSRDISNYDPIGQFYYV
                     ELAYKF"
     gene            complement(1115..1663)
                     /locus_tag="VD737_00415"
     CDS             complement(1115..1663)
                     /locus_tag="VD737_00415"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HXW09051.1"
                     /translation="MRRRVARLLGAAVVGLVVIVPTGHAEAPADAPVDRPPGTLPLSA
                     FPQERIAVETRASFRRRLFLAWRAETYAALAQGLMYVPDAAMRPDQAMIFVYDPPQRV
                     SMWMKNTLQSLDMLFVDRRGCIVTIKERAVPRSPATIDSRAPVALVVELKGGTVADQG
                     IAVGDRVVRIDAGWPSEETSCR"
     gene            complement(1666..2271)
                     /locus_tag="VD737_00420"
     CDS             complement(1666..2271)
                     /locus_tag="VD737_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09052.1"
                     /translation="MLLATTTAGCHADIAATGGALPPEARARQGTPLAADGSSPAMPA
                     AAEALCRPAPSGYLRARLQGAIEADLDWSSPTTTQCIGGPRPTADGLRLVFKGTAGAD
                     PLLVIIGVGVGEAVTAARNVPASVTVVREGAGEFFATQGDDKCAVDELSQDPVAGQLG
                     RYRFTGRGYCVQPARAVGDGANAVLVSRFDVDAIVEYPREP"
     gene            complement(2300..3220)
                     /locus_tag="VD737_00425"
     CDS             complement(2300..3220)
                     /locus_tag="VD737_00425"
                     /inference="COORDINATES: protein motif:HMM:NF025168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HXW09053.1"
                     /translation="MTAATPRTVEQYLDLLRNELQGSDPALIQDALYDAEEHLRAELA
                     QDPGATQETMLERIVASYGAPAEVADAYRTNEARVQAALRTPPPRPKRSAIGRFFAVY
                     SDPRAYLSVAYMLLALATGILYFTFAVTGSALTVGLAVLIIGVPFFLLFIGSARVLAL
                     AEGRIVESLLGVRMPRRPVHPGPRMNWWNRIVEMVGDPRTWGTLLYLVAMLPLGIFYF
                     VFAVVGIVVSLSLFIAPIVVLLHVTGMITLDGTVNGPHPALLPLVSILGIVLLTITLH
                     LSRGIGYLHGQLAKYLLVRPAPTVDEAVAA"
     gene            complement(3217..3639)
                     /locus_tag="VD737_00430"
     CDS             complement(3217..3639)
                     /locus_tag="VD737_00430"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXW09054.1"
                     /translation="MERNEPALMADALADPAAELQARKFQRELSAGLTALVLLSVLAD
                     ADSDLYGYEIARRLSTDAMPSAPFKQGALYPVLRNMSSAGLLTSRVVPSYSGPPRRYY
                     RITPLGRDVLAAWRETWLATRAFVDTVVHPITAQETSP"
     gene            3783..5063
                     /gene="glgC"
                     /locus_tag="VD737_00435"
     CDS             3783..5063
                     /gene="glgC"
                     /locus_tag="VD737_00435"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HXW09055.1"
                     /translation="MSTEHHPIVAPRRYLSRLTRGTLAVIMAGGRGERLKQLTEDRCK
                     PAAPFGGKFKIIDFVLSNCLNSGIRQISVLTQYKAHSLIQHMGKGWGFLRGEFGEFIE
                     IIPAQQRVGPQWYQGTADALWQNMDLIRAHRPLHVLVLAGDHIYKMDYGPMIGFHVEK
                     EADVTVGVVEVPRDRAREFGVVGVDESNRVLDFLEKPGDPPPMPGRPDIALASMGIYV
                     FNPRLLERMLRADAENEASAHDFGKNIIPEAIDNLRVFAYPFEDVRTKAQNYWRDVGT
                     VDAYYEANLELVRVSPELNIYDEQWPIWTYQEQLPPAKFVFDDPDRRGCAIDSMVSGG
                     CIISGSRVANSLLFSSVRVHDHSSIERSVILPRVRVGAHCVIRNAIVDEGTAIPDGMQ
                     IGVDVQADRERFYVSEGGVTLVTASMLRAAPARG"
     gene            5114..5644
                     /locus_tag="VD737_00440"
     CDS             5114..5644
                     /locus_tag="VD737_00440"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HXW09056.1"
                     /translation="MLNTLPPSRWRTPSIVAWSMLVLLSGGWLGGCSALAPKLETPQL
                     SLLGIQMLSTDMFAQKFRVRVKVENPNDLELPVRGLDYKIILMGDSFADGQSSDRFVV
                     PAKGEAEFDMVVTTNFVSSLGRLISRVGGGKLENLDYEIAGEVLLDKGFVKKIPFNHH
                     GQVDLARATGRKSGEI"
     gene            5766..6596
                     /locus_tag="VD737_00445"
     CDS             5766..6596
                     /locus_tag="VD737_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXW09057.1"
                     /translation="MQHLVRSVLLHGIRLPYVEHGDPDGVPVVFLHGYTDSWRSYETV
                     LPNLPRSIRAFALTQRGHGDADRPATGYRPQDFARDVAGFLDAHGIERAVVVGHSMGG
                     SVAQRFAIDYPDRLAGLILVGARASWHDQPDVAGLIEHVTSSLGDTVDPQFAREFQQS
                     TIAQPVPPGWLEIAIAESLKLPASTWRAVCEQGVAQADLTGLLVRIDAPTLLVCGDLD
                     SYARDGQQALLQGIADSRLEWYAGAGHALHWEEPQRFARQVVEFVRQVGAIERRLATA
                     "
     gene            complement(6636..8105)
                     /locus_tag="VD737_00450"
     CDS             complement(6636..8105)
                     /locus_tag="VD737_00450"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HXW09058.1"
                     /translation="MARHRICYLTAEISPFAKMGGLGDVASALVRRLHRRGHEVLAFA
                     PLHGVMELNGIDRRPVASLQGITVRVGNHDYAYDVHRARLPGGDDGWVYLVDCPEVFD
                     RPQIYSEAPDEHLRYLVLTRAALECCQRLAFAPDIVHCNDWHTALAPLLLRTAYAWDR
                     QVFGATRSVFTIHNIGYQGVFPASAAADVGPGVGLEALHQGDLEQGRINPMRHGILYA
                     DAVTTVSPTYAQEIRTPEGGYGLDADLRSRSDAVHGILNGVDYEEWNPATDRYLPFRY
                     DAADLAGKARNKEALLDLLRMPAASSTPLLGMVTRLTRQKGIDLLFEVLPELLQHREL
                     RFVALGSGEPQYEQFMASLQERFPGKAVFYTGYSEELAHYIEAAADMFLMPSLYEPCG
                     LNQMYSLRYGTVPIVRRTGGLADSVLHFDPATGQGTGVVFNDFDPGGLRWGITTALEW
                     FSWPSVWRRLVQNGMAQDFGWDSSAARYESLYDTLGDRG"
     gene            8188..>8369
                     /locus_tag="VD737_00455"
     CDS             8188..>8369
                     /locus_tag="VD737_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXW09059.1"
                     /translation="MQDRTTTTRRDGSRDFERIARAIEYIATHAHEQPSLADIAAAAG
                     WSEYHFARVFRRWAGIS"
BASE COUNT         1352 a         2827 c         2875 g         1315 t
ORIGIN      
        1 tggcagccga tgggcgagac gttcgcgatc cgcgcctcgt actcggagtc gttcacggcc
       61 ccgacgctgt acgagctgtt cggaccgact agccaaggct ttacgtcgag cctctcgctc
      121 aatcaatacg attcgagcgg aaactcactt gggaccaccg acacgccggc gcaatatcgc
      181 agccagtccg gttccaacac gcagctcgac ccttcggagt cgaccaacta ctcggtcggc
      241 ttcgagtgga acccggaagg cgccatgcag ggcctgcaga tcggcgtgga ctactggtcg
      301 atcgaggaag aggacatcgt cgacgtcctg ccctcgtcga tcgtgctgca gtcggtcgag
      361 gcgctgggtc cggcctcgcc gtacgcctcg tacgtccgtc gcggcgtctc gtcggccggc
      421 gaagtgtact tcggcacggg cacgccgatc tcggcgcccg gcgagatcac ggggtcggtg
      481 ggcgacacgg tctggttgag caactcgttg atcaacctgt ccagcatcga gcaggacggc
      541 ctggacgtgc ggatcggcta ctcgttcgag acggagacgg ccggtacgtt ctcggcgcag
      601 ttcatctcga cgttcctgtt caactacgac tcgctgccgg ttccgacgga accgctgatc
      661 gagctgtccg ggacctacca cgacgactac ggcctgttcc cggactaccg ggcgttcctg
      721 cagctcggct gggcgatggg cggcttcacg ctgggcatca acgggcagta cctgcccgag
      781 ggtgacgacg tgacgtcgca ggagccgccg tacgtgaagt tcgacgacta catgtcgtgg
      841 gacatccgtg cgggctacga cttcacggag cagggcttcc cgctgtcgtt caatgccggc
      901 gtgaacaacg tgttcgacga agagccggtg ttcatcgagt cggaaggcaa ccagagccgc
      961 gacatcagca actacgaccc gatcggccag ttctactatg tcgaactagc ctacaagttc
     1021 tgataacgat cggaacgagt cgttgactcg agggccggct cgcaagagcc ggcccttttt
     1081 ttttgtctcg aggcgcctgc gcgatcgcgc cgggtcaccg gcaactggtt tcctcgctcg
     1141 gccagcccgc gtcgatgcgc acgacgcgat cgccgacggc gattccctgg tcggccacgg
     1201 tgccaccctt caattcgacg accagcgcca cgggcgcacg cgagtcgatg gtcgcgggcg
     1261 agcgcggcac cgcgcgctcc ttgatcgtga cgatgcatcc acgccggtcg acgaacagca
     1321 tgtcgagcga ctgcagcgtg ttcttcatcc acatggacac gcgctgcggc gggtcgtaga
     1381 cgaagatcat cgcctggtcg ggccgcatcg cggcgtcggg cacgtacatc aggccctgtg
     1441 ccagcgcggc gtacgtctcg gcacgccagg ccaggaacag ccgccggcga aaactcgcgc
     1501 gcgtctcgac cgcgatcctt tcctgcggga acgcgctcag cggcagtgtg ccgggagggc
     1561 ggtcgacagg cgcatcggcg ggagcctcgg cgtggcccgt cggcacgatc acgacgagac
     1621 ccacgacggc ggcaccgagc agcctggcca cgcgacgcct cacgatcacg gctccctggg
     1681 atactcgacg atcgcgtcca cgtcgaagcg cgagacgagc acggcgttcg caccgtcgcc
     1741 gacggcgcgc gcgggctgca cgcaatagcc gcgcccggtg aaccggtaac gaccgagctg
     1801 cccggcgacc gggtcctggc tcagctcgtc cacggcgcac ttgtcgtcgc cctgggtcgc
     1861 gaagaattcg cccgcgcctt cgcgcacgac ggtcacgctc gccgggacgt tgcgtgcggc
     1921 cgttacggct tcgccgacgc cgacgccgat gatcacgagc agcggatccg cgccggcggt
     1981 gcccttgaag acgagacgca gcccatcggc ggtcggtcgc ggaccgccga tgcactgcgt
     2041 cgtcgtcggg ctggaccagt cgagatctgc ctcgatcgcg ccctgcagcc tcgcgcgcag
     2101 gtatccggac ggtgcagggc ggcagagcgc ctcggcggcc gcgggcatgg ccgggctcga
     2161 tccgtcggcc gccagcggcg tgccctggcg tgcccgggcc tcgggcggca gggcgccgcc
     2221 ggtggccgcg atgtccgcgt ggcagccagc ggtcgtcgtc gccagcagca acgccgccca
     2281 cgcggcgccg ccgcggcggt caggcggcga cggcctcgtc gacggtcggt gcgggacgca
     2341 ccagcagata cttcgccagc tggccatgca ggtacccgat accccgggag agatgcagcg
     2401 tgatggtgag cagcactatc cccaagatcg agaccagcgg caacaacgcg gggtggggtc
     2461 cgttcacggt gccgtcgagc gtgatcatgc cggtcacgtg caggagcacc acgatcggcg
     2521 cgatgaaaag cgacagcgac accacgatgc cgaccacggc gaacacgaag tagaagatcc
     2581 cgagcggcag catcgcgacc aggtacagca gcgtgcccca ggtgcgcggg tccccgacca
     2641 tctcgacgat gcggttccac cagttcatgc gcgggccggg gtgcaccggg cgtcgcggca
     2701 tgcgcacgcc gagcaacgat tcgacgatgc gtccctcggc gagcgcgagc acgcgcgcgg
     2761 aaccgatgaa cagcaggaag aacggcacgc cgatgatcag gaccgcgagt ccgaccgtca
     2821 gcgccgagcc ggtgaccgcg aacgtgaagt agaggatgcc ggtcgccagc gcgagcagca
     2881 tgtaagccac gctcaggtag gcgcgcggat cggagtacac ggcgaagaac cggccgatcg
     2941 cggagcgctt cggccgcggt ggcggcgtgc gcagcgccgc ctgcacgcgc gcttcgttgg
     3001 tgcgataggc gtcggccacc tcggccgggg cgccgtagct cgcgacgatg cgctcgagca
     3061 tggtttcctg ggtggcgccc gggtcctggg cgagctcggc gcgcaggtgc tcctcggcgt
     3121 cgtagagcgc gtcctggatc agcgcggggt cggatccctg cagttcgttg cggagcaggt
     3181 cgaggtattg ctcgacggtg cgcggcgtcg cggccgtcat ggcgaggtct cctgtgcggt
     3241 gatcgggtgg acgacggtgt cgacgaacgc gcgcgtggcg agccacgtct cgcgccaggc
     3301 cgcgaggacg tcgcgtccga gcggcgtgat ccggtaatag cggcggggcg ggcccgagta
     3361 cgatgggacg acccgactcg tcagcagccc cgcgctgctc atgttacgga gcacggggta
     3421 cagcgcaccc tgcttgaacg gcgcagacgg cattgcgtcg gtgctgaggc gcctcgcgat
     3481 ctcgtaaccg tagaggtcgg aatcggcgtc ggcgagcacc gaaagcagga ccagtgccgt
     3541 gaggcctgcg ctcagctcac gctggaattt gcgcgcctgc agttccgccg ccgggtccgc
     3601 cagcgcgtcg gccatcaggg ccggttcgtt ccgctccatg cggctcgctc ccctagtcac
     3661 ttcggcatat tagtaatcaa acactagtac gaagtcaaca gtatcgtccg gtgaacgcga
     3721 ggggagatgg tattgctgcc cgcgccgact atgtaacatc agcggtcgcc caaggaggca
     3781 ggatgtccac ggaacaccac cccattgtcg cgccgcggcg ttacctgagc cggctcaccc
     3841 gcggcacgct ggcggtgatc atggcgggcg ggcgaggcga acggctcaag caactgacgg
     3901 aggaccgctg caagccggcg gcaccgttcg ggggcaaatt caagatcatc gacttcgtgc
     3961 tttcgaattg cctcaattcg ggcattcggc agatctcggt cctgacccag tacaaggcgc
     4021 actcgctgat ccagcacatg ggcaagggct gggggttcct gcgcggcgag ttcggcgagt
     4081 tcatcgagat catccccgcg caacagcgcg tcgggccgca gtggtaccag ggtacggccg
     4141 acgcgctgtg gcagaacatg gacctgatcc gggcgcaccg gccgctgcac gtcctcgtgc
     4201 tcgccggcga ccacatctac aagatggact acggcccgat gatcggcttc cacgtcgaga
     4261 aggaagccga cgtcacggtg ggcgtcgtcg aggtgccgcg cgaccgcgcg cgtgagttcg
     4321 gcgtggtggg ggtcgacgag tcgaaccgcg tgctcgactt cctcgagaag ccgggcgacc
     4381 caccgccgat gcccggccgg cccgacatcg cgctggcgtc gatgggcatc tacgtgttca
     4441 acccgcgcct gctcgaacgc atgctgcgcg ccgatgcgga gaacgaagcg tccgcgcacg
     4501 acttcggcaa gaacatcatc ccggaggcga tcgacaacct gcgcgtgttc gcctatccgt
     4561 tcgaggacgt gcgcaccaag gcgcagaact actggcgcga cgtcggcacg gtcgacgcct
     4621 actacgaggc gaacctcgag ctcgtgcgcg tctcgcccga actcaacatc tacgacgagc
     4681 agtggccgat ctggacgtac caggaacagc tgccgccggc caagttcgtg ttcgacgatc
     4741 ccgaccggcg cgggtgtgcg atcgactcga tggtctcggg tggctgcatc atctccggtt
     4801 cgcgcgtggc caattcgctg ctgttctcga gcgtgcgcgt ccacgaccac agctcgatcg
     4861 agcgctcggt gatcctgccg cgcgtgcgcg tcggcgcgca ctgcgtgatc cgcaacgcga
     4921 tcgtcgacga agggacggcg atccccgacg gcatgcagat cggcgtcgac gtgcaggccg
     4981 accgggagcg tttctacgtc agcgagggcg gcgtgacgct cgtcacggct tcgatgctgc
     5041 gcgcggcgcc ggcgcgcgga tgagtcctaa ggtggccgtg cgccacgtca gtcacactcg
     5101 ggagttgcac cgcatgctga acacgttgcc gccaagccgc tggcggacgc cgtcgatcgt
     5161 ggcctggtcc atgctggtcc tgctgtccgg cgggtggctc ggtggctgct ccgcgctcgc
     5221 ccccaagctc gaaaccccgc agctgagcct gctcggcatc cagatgctgt cgacggacat
     5281 gttcgcgcag aaattccgcg tgcgcgtgaa ggtcgagaac cccaacgacc tcgaattacc
     5341 cgtccgcggg ctcgactaca agatcatcct gatgggcgac agctttgccg acggccagag
     5401 cagcgaccgc ttcgtcgttc ctgccaaggg cgaggccgaa ttcgacatgg tggtgaccac
     5461 caacttcgtc agcagcctcg gccggctgat cagccgcgtg ggcggcggca agctcgagaa
     5521 cctcgattac gagatcgcgg gcgaggtgct gctcgacaag ggcttcgtca agaagatccc
     5581 gttcaaccac cacgggcagg tggacctcgc gcgcgcgacg ggcaggaagt ccggggagat
     5641 ctgacgcagg cggtcgccgc gcgggcggcg caacgctttc acgggtcttt cacgcccgtg
     5701 gcgacggtct gttcacgccc gttccgtacg gtgtgcgctc cgtaaatcct ccggagtaca
     5761 tcgtcatgca acacctcgtc cgctcggttc tcctacacgg tatccgcctg ccctacgtcg
     5821 agcacggcga ccccgacggg gtaccggtcg tcttcctgca cggctacacg gattcgtggc
     5881 ggtcgtacga gaccgtgctg ccgaacctgc cccggtccat acgcgcgttc gcgctgacgc
     5941 agcgcgggca cggcgacgcc gatcggccgg cgacgggcta ccgcccgcag gatttcgcgc
     6001 gcgacgtcgc aggcttcctg gatgcgcatg gcatcgagcg cgcggtcgtc gtgggtcact
     6061 cgatgggcgg ctcggtggcg cagcggttcg cgatcgacta cccagaccgt ctcgccgggc
     6121 tgatcctggt cggtgcgcgt gcctcgtggc acgatcagcc cgatgtcgcg ggcctgatcg
     6181 agcacgtcac atcgagtctc ggcgacacgg tcgatccgca gttcgcgcgc gagttccagc
     6241 agagcaccat cgcgcagccg gtgccacccg gttggctgga aattgcgatc gccgagagct
     6301 tgaagcttcc ggccagcacg tggcgggccg tgtgcgagca gggcgtggcg caggcggatc
     6361 tgaccgggct gctcgtccgg atcgacgcgc cgacgctgct ggtctgcggc gacctcgact
     6421 cgtacgcccg tgacggacag caggcgctgc ttcagggtat cgcggattcg cggctcgagt
     6481 ggtacgcggg cgccgggcac gcgctgcact gggaagagcc ccagcggttc gcccggcagg
     6541 tcgtggagtt cgtgcgccag gtcggcgcca tcgaacggcg cctggccacc gcctgacgcc
     6601 aggggatcgg ggccgggtcg cagtcgcgcg ctgtcctagc cccgatcccc taacgtgtcg
     6661 tacagcgatt cgtaccgcgc cgcgctcgaa tcccagccga agtcctgcgc catgccgttc
     6721 tgcacgagcc gtcgccagac gctcggccag ctgaaccact cgagcgccgt ggtgatgccc
     6781 cagcgcaggc cgccggggtc gaaatcgttg aacacgacgc cggtgccctg gcccgtcgcc
     6841 ggatcgaaat gcagcacgct gtcggcgagc ccgccggtgc gccgtacgat cggcacagtg
     6901 ccgtagcgca ggctgtacat ctggttgagc ccgcagggct cgtacagcga gggcatcagg
     6961 aacatgtcgg ccgccgcctc gatgtagtgc gcgagctcct cgctgtagcc tgtgtagaac
     7021 acggccttgc cggggaagcg ctcctgcagc gacgccatga actgctcgta ctgcggctcg
     7081 ccgctgccga gcgccacgaa ccgcaactcg cgatgctgca gcaactcggg caacacctcg
     7141 aacagcaggt cgatgccttt ctggcgcgtc aggcgcgtca ccatcccgag cagcggcgtc
     7201 gaactggcgg ccggcatgcg cagcaggtcg agcagcgcct ccttgttgcg cgccttgccc
     7261 gcgaggtcgg cggcgtcgta gcgaaacggc aggtatcggt cggtggccgg gttccactct
     7321 tcgtagtcga ctccgttcag gatgccgtgc accgcgtcgc tgcgcgagcg caggtcggcg
     7381 tcgagcccat agccgccctc gggggtgcgg atctcctgag cgtacgtcgg gctcaccgtg
     7441 gtgacggcgt ccgcgtacag gatgccgtgc cgcatcgggt tgatgcggcc ctgttccaga
     7501 tcaccctggt gcagcgcctc gaggcctacg ccgggcccca cgtccgcggc cgcgctggcc
     7561 ggaaagacgc cctggtagcc gatgttgtga atcgtgaaca cgctgcgcgt cgcgccgaac
     7621 acctggcggt cccacgcgta agccgttcgc agcagcagcg gcgccagcgc cgtatgccag
     7681 tcgttgcagt gcacgatgtc gggggcgaac gcgagccgct ggcagcactc cagggccgcg
     7741 cgcgtcagca cgaggtagcg caggtgctcg tcgggggcct cggagtagat ctgcggtcgg
     7801 tcaaacactt ccgggcagtc gaccaggtac acccagccgt cgtcgccgcc cggcaacctc
     7861 gcgcgatgca cgtcataggc gtagtcgtgg ttgccgaccc gcaccgtgat gccctgcagc
     7921 gacgcgaccg gccggcggtc gatgccgttc agttccatca cgccgtgcag gggcgcgaag
     7981 gcgagcacct cgtggccgcg ccggtgcagg cggcgcacga gcgcactcgc cacgtcgccg
     8041 agccccccca tcttggcgaa gggcgagatc tcggccgtga ggtagcagat ccggtgacgg
     8101 gccatgagcc ccgtattctg tcacagcgtc cttgcgcgac agcacgatcc gggtggccgc
     8161 cgggcgcggg gtgggtatcc ttgcgccatg caggaccgga ccaccaccac gcgtcgcgac
     8221 ggctcgcggg atttcgagcg aatcgcgcgc gccatcgaat acatcgccac ccacgcgcac
     8281 gagcagccct cgctggcgga catcgccgcc gccgcgggct ggagcgaata tcacttcgcg
     8341 cgcgtcttcc gtcggtgggc cggcatctc
//
LOCUS       DASPFR010000020        10303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_617197, whole genome shotgun sequence.
ACCESSION   DASPFR010000020 DASPFR010000000
VERSION     DASPFR010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10303
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617197"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1948
                     /locus_tag="VD737_00460"
     CDS             <1..1948
                     /locus_tag="VD737_00460"
                     /inference="COORDINATES: protein motif:HMM:NF014312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HXW09060.1"
                     /translation="PTGVGMPGAFPPLAASDYLLQDKDRAVAVVVRGLQGEVTVNNQK
                     YNSVMPAMTQLSDQQIADVLTYVLNSWGNKGGAIPVERVVAERAAALKEGITPLSPTQ
                     HATTVGETQYQGGAGSPVAGADAQIRVTPGAPNMTMAEFDRAQQIYFQRCAGCHGVLR
                     KGATGKPLTPDITQPKGTEYLKVFINQGSPAGMPSWGKSGELSPEEVDIMARFVQHEP
                     PQPPEFGMKDIKGTWKVLVPVDQRPTSQQNKYNLDNVFAVTLRDAGEVALIDGDTKQI
                     INVIKTGYAVHISRLSHSRRYVYTIGRDGRIDLIDLWMAKPDRVAEIKIGLEARSVET
                     SKYKGYEDKYAIAGAYWPPQYTIMDGATLEPLKVVSTRGMTVDTQEFHPEPRVAAIVA
                     SHEHPEFIVNVKETGQILLVNYEDIVNLKTTTIGAARFLHDGGWDASKRYFLTAANQS
                     DKIAVVDSRDQKLAALVDVDKIPHPGRGANLTDPQFGPVWVTSALGNEKVTLIGTDPE
                     GHKDSAWKAVRVLKGQGGGSLFVKTHPRSKHLWVDTPLNPDAKISQSVAVFDITNFDA
                     GYKVLPIAEWAKLGEGPKRVVQPEYNAAGDEVWFSVWNGKDQKSAIVVVDDKTLELKK
                     VIDDPRIVTPTGKFNVYNTGADVY"
     gene            complement(2062..2691)
                     /locus_tag="VD737_00465"
     CDS             complement(2062..2691)
                     /locus_tag="VD737_00465"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HXW09061.1"
                     /translation="MYLPAHFAETEPEELHRIIHEHPLGALVTSTEAGLDANHVPFIL
                     DASRGPHGTLVAHVARANPAWTEVADGASVLVIFRGAQGYISPNWYLSKQETHRHVPT
                     WNYEVVHVHGRLRVHDDPSTVGAVVARLTRQHEASQPRPWKMSDAPADFLTDKIRQIV
                     GIEVEITRLEGKRKLSQNREHRDLESAVQALEEQGQGTLAAAMKRTATP"
     gene            2853..3200
                     /locus_tag="VD737_00470"
     CDS             2853..3200
                     /locus_tag="VD737_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXW09062.1"
                     /translation="MNHQDIVRALAALAQDSRLAVFRLLVKRGPEGYSAGEIGEKLDI
                     PGPTLSFHLKELAQAGLITPRKASRFIYYAPAFDRMNELLGYLTENCCSQGAACATAC
                     VPNTTAQRKRKSA"
     gene            3197..3652
                     /locus_tag="VD737_00475"
     CDS             3197..3652
                     /locus_tag="VD737_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HXW09063.1"
                     /translation="MKRFHVHVSVKNLDESIRFYSTLFGTAPSVEKPDYAKWMLDDPR
                     VNFAISTRDPKHGVNHLGLQVQTDQELASLEGQLRKADATMVTECGTTCCYANSDKYW
                     VTDPQGVAWETFRTLGNAPVYGESPVATARKTECCAPPQAVAQAAGCCG"
     gene            3601..4110
                     /gene="arsN2"
                     /locus_tag="VD737_00480"
     CDS             3601..4110
                     /gene="arsN2"
                     /locus_tag="VD737_00480"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HXW09064.1"
                     /translation="MLRTAAGRCTGRRLLRMMDTVTPRPESADGADFAAIRALLEREG
                     LPNVDLGTGPGAGFWVLRDSGRVIGAIGLETYGRSGLLRSLVVAPEARGTGLGRQLVE
                     AVEDAARSRGLGRLVLLTETAERFFAGRGYTVIDRATAPDEVRASAEFRSLCPASAIC
                     MSKTLATAS"
     gene            4107..4586
                     /locus_tag="VD737_00485"
     CDS             4107..4586
                     /locus_tag="VD737_00485"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HXW09065.1"
                     /translation="MNVLFLCTGNSCRSILGEATFNHLAPAGWRAMSAGSRPTGSVHP
                     RSLALLQREGISTEGAHSKSWDDLPATPDIVITVCSSAAGETCPAYLGSVLRVHWGVD
                     DPAHATGTDDEIDAAFVRAYRTLRRRIEAFVALPLDQLKNDRASLERAMDEIGKKVQ"
     gene            4589..5662
                     /gene="arsB"
                     /locus_tag="VD737_00490"
     CDS             4589..5662
                     /gene="arsB"
                     /locus_tag="VD737_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HXW09066.1"
                     /translation="MSIQCEATVKHAVVAPMSVFERYLTVWVFVCILLGISAGQLFPE
                     VFQAIGRMEVARVNLPVGLLIWVMIIPMLMRVDFGALHQVRRHVRGIGVTLFVNWLVK
                     PFSMALLAWIFVRHLFAPWLPADQLDSYVAGLILLAAAPCTAMVFVWSRLSNGDPLFT
                     LTQVAVNDAIMVVAFAPIVGLLLGIAAITVPWNTLITSVVLYIVIPVILAQWWRRRLL
                     RRGQAAFDAAMARIGPWSITALLLTLVLLFAFQGEAIIDQPLVIALLAVPILIQVFFN
                     SSLAYWLNRAAGEQHAIACPSALIGASNFFELAVAAAISLFGFESGAALATVVGVLIE
                     VPVMLLVVRVVNSTKGWYERGLA"
     gene            5662..6960
                     /locus_tag="VD737_00495"
     CDS             5662..6960
                     /locus_tag="VD737_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09067.1"
                     /translation="MAASTSVGAPLAASLNEACHCIWVDRAKLRRQLQADLGQDAGVL
                     DTREGLVSGNVVFIAPQDAARMDGAIVLITRALESEAFQAKAAGAAPAIARARPLASG
                     GLLGFDFHLGGPVPQLIEINTNPGGLLIAFELARAATASCDCLAAPLPQLTAAGVALD
                     RLAARVVEGFRAEWTRARGAGPPLETIAIVDDDPRDQYLYPEFLLYRNLLERSGLRVL
                     VADPAELTASEEALLCRGERIDLVYNRLTDFYLADDRHAALRQAYVRDLVVVTPHPAA
                     HARWADKRLLAWLRDVSMLEDAGLSGAERRAVLEAIPPTEIVEPCNAGDLWARRKTLF
                     FKPIDGFAGKAAYRGDGLTRTAFEHVLSHRYIAQAIAPTSFRRVAPVDGTGTTELRVD
                     VRNFAAHGETWLRAARLYRGQTTNFRTAGGGFAPVLTLRS"
     gene            complement(6995..9220)
                     /locus_tag="VD737_00500"
     CDS             complement(6995..9220)
                     /locus_tag="VD737_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HXW09068.1"
                     /translation="MRDPAPPRASGLALAMAGLLLAVGGGAATAAPPAVATEDGLETV
                     TVYARRITPVTRVAATVTVLDAARIEATLASDVRELVRYEPGLTVRNDPFRFGLDTVS
                     VRGLGGNRVAVEIDGVPAAGGFAVGSYADSGRAFLDPAFVHRVEFLRGPASSLYGSEA
                     IGGVVAMTTYMPAHLLRSGSEQVASKVAAGYDSQDSGWHAVALLAGRAGPGEWLLGYV
                     RREGHESDTAADVEPDPRDYVNDALLAKYVLPDAPGGALTLAFDAGRHEQETAVDAFL
                     GVDRFVNTTRLDGDDRLERFRASVAQTLSSGRAWFDTADWRLYWQGTATDQDTYEVRR
                     AVPPRSPAVVLERSFDYDEATLGLEFTAARALSARSFEHQLVYGVEATRTRLEEMRDG
                     LQTTIDTGATSGVILGETFPLRDMPVSDVTEIGVFLQDEIELAGARWTLVPALRIDHY
                     DLSPDVDRIYREDNPSSPAVSIEETSLAPKLGATYRFTDVITGFFQYAHGFRSPPPED
                     VNIGLEIPLFRVRAVPNPDLKPETSDGFELGLRVRSAAVTLTASAFRTDYDDFIESKV
                     NLGADASGVTLFQSRNVAEARIYGAEMSALANMGAATARLDGWTARLAASWTRGDDLV
                     RDKPLNSIDPPRLVAGFRYEAPSQRWSSEIVVTAVEAQRDVDTSLADLYRTDGHATVD
                     LLAEWRVSPRTRLNVGLFNLTDEAYIEWADVRGRTAADVLVPYYTQPGFNAAATLHYD
                     F"
     gene            9440..>10303
                     /locus_tag="VD737_00505"
     CDS             9440..>10303
                     /locus_tag="VD737_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXW09069.1"
                     /translation="MKLETLAVHAGFAPDPTTRAVAVPIYQTTSYAFDDTQHGADLFD
                     LKVVGNIYTRIMNPTTDVLEKRIAALEGGVGALALASGQAAITYAIQTITEAGQNVIS
                     SSALYGGTYNLFAHTFPQYGIHVKFADHREPASFEPLIDTNTRAIFCESVGNPAGNVT
                     DFAALAEIAHRHGIPLIVDNTVPSPYLCRPFEHGADIVVHSLTKFIGGHGTSIGGLLV
                     DGGTFDFGASGRFPNFTEPDPSYHGLAYFPALGHGAFILKARVQMLRDMGLAISPFNA
                     FLILQGIETLSL"
BASE COUNT         1684 a         3553 c         3427 g         1639 t
ORIGIN      
        1 gccgacgggt gtcggcatgc cgggcgcctt cccgcctctc gcggcgtccg attacctgtt
       61 gcaggacaag gaccgcgcag tcgccgtggt cgtgcgcggc ctgcagggcg aggtcacggt
      121 caacaaccag aaatacaact cggtcatgcc tgccatgacg caactgtcgg accagcagat
      181 tgcagacgtg ctgacctacg tgctgaattc ctggggcaac aagggcggcg cgatcccggt
      241 cgagcgcgtc gtagcggaac gtgccgcggc gctcaaggaa ggcatcacgc cgctctcgcc
      301 gacacagcac gcgaccacgg tcggtgaaac gcaataccag ggcggcgcag gctcgccggt
      361 cgctggggca gacgcgcaga tccgcgtcac gccgggcgcg ccgaacatga ccatggccga
      421 gttcgaccgc gcccagcaga tctacttcca gcgctgcgcc ggatgccacg gcgtgctgcg
      481 caagggcgcc acgggcaagc cgctaacgcc cgacatcacg cagcccaagg gcaccgaata
      541 cctgaaggtg ttcatcaacc agggctcgcc ggccggcatg ccgagctggg gcaagtcggg
      601 cgagctctcg ccggaagaag tggacatcat ggcgcgcttc gtccagcacg agccaccgca
      661 gccgcccgag ttcggcatga aggacatcaa gggcacctgg aaggtgctcg tgccggtcga
      721 ccagcgaccg acgtcccagc agaacaagta caacctcgac aacgtcttcg cggtgacgct
      781 gcgcgacgcc ggcgaggtcg cgttgatcga cggcgacacc aagcagatca tcaacgtcat
      841 caagacgggc tatgcggtgc acatctcgcg cctgtcgcac tcgcgccgct acgtgtacac
      901 gatcggacgc gacggccgca tcgacctgat cgacctctgg atggcgaagc ccgaccgcgt
      961 cgccgaaatc aagatcggac tcgaggcgcg ctcggtcgag acgtccaagt acaagggcta
     1021 cgaggacaag tacgcgatcg ccggcgccta ctggccgccg cagtacacga tcatggacgg
     1081 cgcgacgctc gagccgctca aggtcgtctc gacgcgcggc atgacggtcg acacgcagga
     1141 gttccacccg gagccgcgcg tggccgccat cgtcgcctcg cacgagcacc cggagttcat
     1201 cgtcaacgtg aaggagacgg ggcagatcct gctcgtcaac tacgaggaca tcgtcaacct
     1261 caagaccacc acgatcgggg ccgcgcgctt cctgcacgac ggcggctggg acgccagcaa
     1321 gcgctacttc ctgacggccg ccaaccagtc ggacaagatc gccgtggtcg attcgcgcga
     1381 ccagaagctg gcggcactgg tggacgtcga caagatcccg cacccgggtc gcggcgccaa
     1441 tctgaccgac ccgcagttcg ggccggtctg ggtcacgagc gcgctcggca acgagaaggt
     1501 cacgctgatc ggtacggacc cggaaggcca caaggacagc gcgtggaagg ccgtgcgcgt
     1561 gctcaagggc cagggcggcg gctcgctgtt tgtcaagacg catcctaggt cgaagcacct
     1621 ctgggtcgac acgccgctca acccggacgc gaagatctcg cagagcgtcg cggtgttcga
     1681 catcaccaac ttcgacgcgg gctacaaggt gctgccgatc gccgagtggg cgaagctcgg
     1741 cgaaggcccg aaacgcgtcg tgcagccgga gtacaacgcc gcgggcgacg aagtctggtt
     1801 ctcggtctgg aacggcaagg accagaagtc cgcgatcgtc gtggtcgacg acaagacact
     1861 cgagctcaag aaggtcatcg acgacccgcg catcgttacg ccgaccggca agttcaacgt
     1921 ctataacacc ggcgccgacg tgtactgacg gttccttcgc gccgggccgg gtctcgctga
     1981 acggcctggc gccgactccc gcgcgctccc gagaagcgcc gttcggcccc gctcttcgga
     2041 gcggggcttt ttttttcgtt gctagggtgt cgcggtgcgc ttcatggctg cggccagcgt
     2101 cccttgtccc tgttcctcca atgcctgcac ggcactctcg agatcccgat gctcacggtt
     2161 ctggctcagc ttccgcttgc cttcgagccg cgtgatctca acctcgatgc cgacgatttg
     2221 acgaatcttg tccgtaagga agtcggccgg ggcgtcactc attttccaag gacgcggctg
     2281 cgaggcctca tgctgccgcg tgaggcgcgc gacgacggcc ccgacggtgc tcgggtcgtc
     2341 atgaacccgc agccggccgt gcacgtgcac gacctcgtag ttccatgtcg gaacgtggcg
     2401 atgggtttcc tgcttgctca ggtaccagtt aggcgagatg tagccctgcg ctccgcggaa
     2461 gatgacgaga acggacgcac cgtcggcaac ttccgtccag gcgggattgg ctcgcgcaac
     2521 gtgcgcgacc agtgttccgt gcggtccccg actcgcgtcg aggatgaatg ggacgtggtt
     2581 tgcgtccaga cccgcctcgg tgctcgtaac cagggctccc aatggatgct cgtgaatgat
     2641 gcgatgcagt tcctcgggct ccgtttccgc gaaatgtgca ggaaggtaca taccggactc
     2701 cgatgagagc gctccgcccc ggcaggaggg tggcccagcg acgatggacc cgaggatacc
     2761 gcgcgcggct ccgctccggc ccacccgccc cggcccgagc gaccgccgga aacatcttga
     2821 ccggtggact atatttctat agatttggaa atatgaatca tcaggacatc gttcgcgccc
     2881 tcgcggcgct ggcccaggac tcccggctcg ccgtcttccg actgctggtc aagcgtggcc
     2941 ccgagggcta ctccgccggc gagatcggcg agaagctcga catccccggc ccgacgctct
     3001 cgtttcacct gaaggagctg gcccaggcgg ggctgatcac gccacgcaag gcgagccggt
     3061 tcatctacta cgcgccggcc ttcgaccgca tgaacgaact gctcgggtac ctgaccgaga
     3121 actgctgcag ccagggcgcc gcgtgcgcca cggcctgcgt gcccaacacc accgcccaac
     3181 gcaagaggaa atcagcatga agcgctttca cgtccacgtg tccgtgaaga acctggacga
     3241 aagcatccgg ttctattcga cgctcttcgg gaccgcaccg agcgtcgaga agccggatta
     3301 cgccaagtgg atgctcgacg atccgcgcgt caacttcgcg atctcgacgc gcgacccgaa
     3361 acacggcgtc aatcacctgg gcctgcaggt gcagaccgac caggaactgg cgagtctcga
     3421 agggcagctc aggaaggccg acgccacgat ggtcaccgag tgcggcacga cctgctgcta
     3481 cgcgaactcc gacaagtact gggtcaccga tccgcagggc gtggcctggg agacgttccg
     3541 gacgctcggc aacgcgcccg tctatggcga gtcgccggtc gcgacggccc ggaagaccga
     3601 gtgctgcgca ccgccgcagg ccgttgcaca ggccgccggc tgctgcggat gatggacacc
     3661 gtcactccca ggcccgaaag cgcggacggg gccgacttcg ccgccattcg cgcgctgctc
     3721 gaacgggagg ggctgccgaa cgtagacctc ggaaccgggc cgggcgccgg attctgggtc
     3781 cttcgtgact ccggccgcgt gatcggcgcg atcggcctcg agacctacgg aaggtccggt
     3841 ctgctgcggt cgctcgtggt ggcgccggag gcgcgtggaa cgggcctcgg acgacaactg
     3901 gtggaagccg tcgaggatgc cgccaggagc cgcggccttg ggcggctggt gctgctcacc
     3961 gagacggcgg agcggttctt cgctggacgc ggctacaccg tgattgatcg tgcgacggcg
     4021 cccgacgagg tccgcgcctc cgccgagttc cggagcctgt gccccgcctc ggccatctgc
     4081 atgtcgaaga ccctggccac ggcgtcgtga acgtactctt cctgtgcacg ggtaactcgt
     4141 gccgctcgat cctgggcgaa gccacgttca accacctggc gccggccggc tggcgagcca
     4201 tgagcgccgg cagccggccc acgggcagcg tccacccccg atcgctcgca ctgctgcagc
     4261 gcgagggcat ctcgacggaa ggtgctcaca gcaagtcgtg ggacgacctg cccgcgacgc
     4321 cggacatcgt gatcacggtg tgctcgagcg cggccggaga aacctgcccg gcctacctcg
     4381 gttcagtgct gcgcgtgcac tggggcgtcg acgacccggc gcacgcgacg ggcacggacg
     4441 acgagatcga tgcggcgttc gtccgcgctt accggaccct gcggcggcga atcgaggcat
     4501 tcgtggccct gcccctcgat caactcaaga acgatcgagc ctcgctcgag cgggcgatgg
     4561 acgagatcgg caagaaggtg caatgacgat gtcgatccaa tgcgaagcga ccgtgaagca
     4621 cgccgtcgtc gcgcccatga gcgtgttcga gcgctacctg accgtgtggg tgttcgtctg
     4681 catcctgctc ggcatctcgg cgggccagct gttccccgag gtgttccagg cgatcggccg
     4741 catggaggtc gcccgggtca acctgcccgt ggggctgctc atctgggtga tgatcatccc
     4801 gatgctgatg cgggtggact tcggcgcgct gcaccaggtt cgccggcacg tccgcggcat
     4861 cggcgtcacg ctgttcgtca actggctcgt gaagccgttc agcatggcgc tgctcgcatg
     4921 gatcttcgtt cgccacctct ttgcgccgtg gctgccggcg gaccagctcg acagttacgt
     4981 ggcagggctg atcctgctcg ccgccgcgcc ctgcacggcc atggtgttcg tctggtcacg
     5041 cctctcgaac ggcgacccgc tcttcacgct cacccaggtc gcggtcaacg acgcgatcat
     5101 ggtcgtggcc ttcgcgccga tcgtcgggct gctgctcggc atcgccgcga tcacggtgcc
     5161 ctggaacacg ctgatcacct cggtcgtgct ctacatcgtg atcccggtga tcctggcgca
     5221 gtggtggcgc cgccggttgc tccgcagagg acaggcagct ttcgacgcgg cgatggccag
     5281 gatcgggccc tggtcgatca cggcgctgct gctgacgctg gtattgctgt tcgcgttcca
     5341 gggcgaggcg atcatcgacc agccgctcgt gatcgcgctg ctggccgtgc cgatcctgat
     5401 ccaggtgttc ttcaactcgt cgctcgccta ctggctgaac cgcgcggccg gcgagcagca
     5461 cgcgattgcg tgtccgtccg cgctgatcgg cgcgtcgaac ttcttcgagc tcgccgtggc
     5521 ggccgccatc agcctgttcg gcttcgagtc gggcgcggcg ctcgccaccg tggtgggcgt
     5581 gctgatcgag gtgccggtca tgctgctcgt cgtccgcgtc gtgaactcga ccaagggctg
     5641 gtacgagcgc ggactggcgt gatggccgcc tccaccagcg tgggcgcacc cctggccgcc
     5701 tcactgaacg aggcgtgcca ctgcatctgg gtcgatcgcg cgaagttgcg ccgccagttg
     5761 caggccgatc tcgggcagga cgcgggtgtg ctcgacacgc gggagggcct cgtatcgggc
     5821 aacgtggtgt tcattgcgcc gcaggacgcc gcgcggatgg acggcgccat cgtgctcatc
     5881 acccgggcgc tcgaaagcga ggcattccag gccaaggctg ccggcgcggc accggcgatc
     5941 gcacgcgccc gaccgctcgc ctcgggcggc ctgctcggtt tcgacttcca cctgggcggc
     6001 cccgtgcccc agctgatcga gatcaacacc aatccgggcg gcctgctcat cgccttcgag
     6061 ctggcgcgag cggccacggc aagctgcgac tgtctcgcgg ctccgctgcc acaactcacc
     6121 gccgcgggcg ttgcactcga caggctcgcg gcgcgggtcg tggaaggttt tcgcgccgaa
     6181 tggacgcgcg cgcgaggagc cggaccgccg ctcgagacca tcgcgatcgt cgacgacgat
     6241 ccccgcgacc agtacctgta tcccgagttc ctgctgtacc gcaacctgct ggagcggtcc
     6301 ggcctgcgcg tgctggtcgc ggatcccgcg gaattgacgg ccagcgagga ggcgctgctg
     6361 tgtcgcggtg agcggatcga cctggtgtac aaccgcctga ccgacttcta cctggcggac
     6421 gaccgccacg cggccttacg tcaggcgtac gtccgggatc tcgtcgtcgt cacgccgcac
     6481 cccgcggcgc atgcccgttg ggccgacaag cgtctgctcg catggttgcg cgacgtatcg
     6541 atgctcgagg acgccggact cagcggcgcc gagcgacggg ccgtgctcga ggcgatacca
     6601 cccaccgaga tcgtcgaacc gtgcaacgcc ggcgacctgt gggcgcgacg caagacgctg
     6661 ttcttcaagc ccatcgacgg cttcgcgggt aaggccgcct accgcggcga cgggctcacg
     6721 cggaccgcgt tcgagcacgt cctcagccat cgatacatag cacaggcgat tgcgcccacg
     6781 tcgttccgac gtgtcgcccc cgtggatgga accgggacga cggaactccg cgtggacgtg
     6841 cggaatttcg ccgcgcacgg cgagacgtgg ctccgggcgg cgcgtctcta ccgcggccag
     6901 accaccaact tccgcacggc cggcggcggt ttcgcgccgg tgctcaccct gcgatcctga
     6961 cccaacggcg tcacgaaacc tgtggcgcca ggattcagaa gtcgtaatgc aaggtcgcgg
     7021 ccgcgttgaa acccggctgc gtgtaatacg gcaccagcac gtcggcggcg gtgcggccgc
     7081 ggacgtcggc ccactcgatg taggcctcgt cggtgaggtt gaacagcccg acgttgagcc
     7141 tcgtccgcgg cgacacgcgc cactctgcga gcaggtcgac ggtcgcatgg ccgtcggtcc
     7201 ggtagaggtc ggcaaggctc gtgtcgacgt cacgctgggc ctcgacggcc gtcacgacga
     7261 tttcgctgct ccagcgctgc gacggcgcct cgtagcgaaa gcccgcgacc agtcgcggcg
     7321 gatcgatcga gttgagcggc ttgtcgcgga cgaggtcgtc gccgcgggtc cacgaggcgg
     7381 ccagccgcgc cgtccagccg tcgaggcgtg cagttgcggc gcccatgttc gccagcgcag
     7441 acatctcggc gccgtaaatg cgggcttcgg cgacgttgcg cgactggaac agcgtcacgc
     7501 ccgaggcatc cgcgccgagg ttcaccttgg attcgatgaa atcgtcatag tcggtccgga
     7561 acgcgctcgc cgtcagggtc acggcggcgc tgcggacacg aaggcccagc tcgaacccgt
     7621 cgctggtctc cggtttcagg tccgggttgg gcacggcgcg cacgcgaaac agcgggatct
     7681 cgagcccgat gttgacgtcc tccgggggcg gcgagcgaaa gccgtgcgcg tactggaaga
     7741 atcccgtgat gacgtcggtg aatcgatacg tcgccccgag tttcggcgcg agcgacgtct
     7801 cctcgatgct gaccgctggc gaggacgggt tgtcctcgcg gtagatgcga tcgacgtccg
     7861 gcgagaggtc gtagtgatcg atgcgcagcg ccggcacgag ggtccagcgt gcgcctgcga
     7921 gctcgatttc gtcctgcaga aagacaccga tctcggtgac gtcgctgacc ggcatgtcgc
     7981 gcagcggaaa cgtttcaccg aggatcacgc ccgacgtggc gccggtgtcg atcgtcgtct
     8041 gcaggccgtc gcgcatctcc tcgagtcgag tccgcgtcgc ttcgacgccg tagaccagct
     8101 ggtgctcgaa ggacctcgcg ctcagggccc tggccgccgt gaattccagg ccgagcgtcg
     8161 cctcgtcgta gtcgaaggac cgttcgagca cgaccgcggg ggagcgcggc ggcacggcgc
     8221 ggcgcacctc gtaggtgtcc tggtcggtgg cggtgccttg ccagtagagc cgccagtcgg
     8281 cggtgtcgaa ccaggcgcgc ccggagctga gcgtctgtgc gacgctcgcc cgaaaccgct
     8341 ccaggcgatc gtcaccgtcg aggcgagtgg tgttgacgaa gcggtccacg ccgaggaacg
     8401 cgtcgaccgc cgtctcctgc tcgtgacgcc ccgcgtcgaa cgcgagcgtg agtgcgccac
     8461 cgggcgcgtc gggcagcacg tacttggcga gcagggcgtc gttgacgtag tcgcgcgggt
     8521 ccggctcgac gtcggcggcc gtgtcgctct cgtgaccttc gcgccggacg tagccgagca
     8581 gccactcgcc cggcccggcc cgaccggcca acagcgcgac ggcgtgccag cccgagtcct
     8641 gcgagtcgta gcccgccgcc accttcgacg cgacctgctc actgccggag cgcagcaggt
     8701 gtgccggcat gtacgtcgtc atggcgacca cgccgccaat cgcttcgctg ccgtacagcg
     8761 acgacgcggg gccccgcagg aactcgacac gatgcacgaa cgcaggatcg aggaacgcgc
     8821 gaccgctgtc cgcgtagctg ccgaccgcga agccaccggc ggcaggcacc ccgtcgatct
     8881 ccacggcgac gcggttgccg cccagtccgc gaaccgaaac ggtgtccagg ccgaagcgga
     8941 acggatcgtt gcgcaccgtc aggccgggtt cgtagcggac gagttcgcgg acgtcggacg
     9001 ccagtgtcgc ctcgatgcgc gctgcatcga gcacggtcac cgtggcggcc acccgcgtca
     9061 cgggcgtgat gcgccgtgcg tagaccgtga cggtctcgag cccgtcttcg gtcgcgaccg
     9121 cgggcggcgc cgccgtcgcg gccccgccgc cgacggccag cagcaggccg gccatcgcca
     9181 gtgccagccc ggacgcacgt ggaggtgcag ggtcacgcat cgcttcgtcg ccttccgtcg
     9241 gtcgccccgg agcaccggaa catgcgctca ggatgccgtg gccctgacgc cacggccatg
     9301 attcagatca agtccagtcg cagtactttc cacacccgtc gtcgatccgg tggtcgcgcg
     9361 tgggcggctg gacgcgcttc ggtacactgc caggtcgccc aggcctctcc ccgcatcacg
     9421 cattggagcc gatcccgaca tgaagctcga gaccctcgcc gtccacgccg gctttgcgcc
     9481 cgaccccacc acccgcgcgg tcgcggtgcc gatctaccag acgacttcct atgcgttcga
     9541 cgatacgcag cacggcgcgg acctgttcga cctcaaggtg gtcggcaaca tctacacgcg
     9601 gatcatgaac ccgaccacgg acgtgctcga gaaacgcatt gccgcgctcg agggcggcgt
     9661 cggagcgctg gcacttgcct ccggccaggc ggcgatcacg tacgcgatcc agacgatcac
     9721 cgaggccggc cagaacgtca tcagctcctc cgcgctctac ggcggcacct acaacctgtt
     9781 cgcgcacacg ttcccgcagt acggcatcca cgtgaagttc gcggaccacc gcgagcccgc
     9841 gtcgttcgaa ccgctgatcg ataccaacac gcgcgcgatc ttctgcgagt cggtcggcaa
     9901 cccggccggc aacgtcacgg acttcgccgc gctcgccgag atcgcccacc gccatggcat
     9961 accgctgatc gtcgacaaca ccgtgccttc gccctacctg tgccggccgt tcgaacacgg
    10021 tgccgacatc gtcgtgcact ccctcacgaa gttcatcggc ggccacggca cgtcgatcgg
    10081 cggcctcctc gtcgacggtg gcacgttcga cttcggggcg agcggtcggt tcccgaactt
    10141 caccgagccc gaccccagct accacggcct ggcgtacttc ccggcgctcg gccacggcgc
    10201 attcatcctc aaggcgcgag tccagatgct gcgcgacatg ggtctcgcga tctcgccgtt
    10261 caacgcgttc ctgatcctcc aggggatcga gacgttgagc ctg
//
LOCUS       DASPFR010000021         2165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1106314, whole genome shotgun sequence.
ACCESSION   DASPFR010000021 DASPFR010000000
VERSION     DASPFR010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106314"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..>2165
                     /locus_tag="VD737_00510"
     CDS             168..>2165
                     /locus_tag="VD737_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXW09070.1"
                     /translation="MSTRRLRSAVVLALAAPAVSFGQATSGQLEEIVVTAQKRAESLQ
                     DIPFSVAAVTEETLRRSGATNIVELARNVSGLAITDLGPGQSQVAIRGISAGQIIRDQ
                     PGVKESVGIYLDESAISVALFTPDLDFYDVERVEVLRGPQGTLFGAGSSSGTLRYITR
                     QPQLGEFGASTELTASTVDDGEFGGSVKGALNLPMGETVAARIVGYYSQLPGFIDSIY
                     PDRGDREDVNGGERTGVRAALRFQPNDQLSITPRIVYQKLETDGFPRIDIWNILGNPY
                     TTTETPVDYGERTQVTQFDEGLDDDFTLGDLNVSYDFGAMSLTSITSYTDRQVQVRRD
                     ASQLTGSVTYDIEGDPGYVSSHVRLDSALVDDTDLEAISQELRLSSNDPDATFRWLVG
                     AFYQQVDRYYRQSLPTPGWDAALGIDNTELNAPPDNPYFSELTYDFEQFAVFGEGTFK
                     FTDQWSLTAGLRYYDFSEDRTITFAGVFADPNIITDVPGTTESDGFSPRVILEFNPTE
                     DVMLTAQVARGFRLGGINDPLNEGLCGSQAEIDTYGGYPIWDDEKTTNYELGTKTRWA
                     DGRVQFNAAAFYTDIDGLQVVVNTSCSSRIILNAQARSVGGELELFARPTDNWDFGLT
                     ATYVNAEITETQRGPDGSPLAGIREGNRLPTSPELQAAASAT"
BASE COUNT          392 a          759 c          679 g          335 t
ORIGIN      
        1 ccgtcgcgag cggtgcccaa gggtcatctg ctgcgctgcg catggctcct cgacgtgacg
       61 acgcacgata gcacgtgccc ccggcgtcgc cgcgcaaaag ccatgttgcg tcttatgttt
      121 gttgttacat actgcgcgcc agttcaaagc ccaggtgagg gaataccatg tcgacaaggc
      181 gattgcgttc cgcagtggtg ctggcgctgg ctgcgccggc cgtctcgttc ggccaggcga
      241 cgtccggtca gctcgaagag atcgtggtga ccgcccagaa gcgcgccgaa tcgttgcagg
      301 acatcccgtt ctccgtggcg gccgtgacgg aggagacgct gcgccgctcc ggcgccacca
      361 acatcgtcga gctcgcccgc aacgtttcgg gcctagcgat cacggacctc ggccccgggc
      421 agagccaggt cgcgatccgc ggcatcagcg ccgggcagat catccgcgac cagcccggcg
      481 tcaaggaatc ggtcggcatc tacctcgacg aatcggcgat ctcggtcgcg ctgttcacgc
      541 ctgacctgga cttctacgac gtcgaacgcg tcgaagtcct gcgcggccca cagggcacgc
      601 tgttcggcgc gggctcgtcg tcgggcacgc tgcgctacat cacgcgccag ccgcagctgg
      661 gtgaattcgg cgcatcgacg gaactcaccg cctcgacggt cgacgacggc gagttcggcg
      721 gcagcgtcaa gggtgcgctg aacctgccga tgggagaaac cgtcgcggcc cgcatcgtcg
      781 gctactactc gcagctgccg ggcttcatcg actcgatcta tccggaccgc ggcgaccgtg
      841 aagacgtcaa cggcggcgag cgcacgggcg tgcgcgcggc gctgcggttc cagccgaacg
      901 accagctctc catcacgcct cgcatcgtct atcagaagct cgagaccgac ggtttcccgc
      961 gcatcgacat ctggaatatc ctcggcaacc cgtacacgac gaccgagacc ccggtcgact
     1021 acggcgagcg cacgcaggtg acgcagttcg acgagggcct cgacgacgac ttcacactcg
     1081 gcgacctcaa cgtcagctac gacttcggcg ccatgtcgct cacgtccatc acgtcgtaca
     1141 ccgaccggca ggtgcaggtg cgccgcgacg cgagccagtt gacgggcagc gtcacctacg
     1201 acatcgaggg cgaccccggc tacgtctcct cgcacgtgcg cctcgactcg gcactggtcg
     1261 acgacaccga cctggaggcg atcagccagg agctgcgcct ctcgtcgaac gaccccgacg
     1321 cgacgttccg ctggctggtc ggcgcgttct accagcaggt ggaccgctac taccggcagt
     1381 cgctgccaac accggggtgg gacgccgcgc tgggcatcga caacaccgag ctgaacgcgc
     1441 caccggacaa tccctacttc tccgaactca cctacgactt cgagcagttc gcggtgttcg
     1501 gcgagggaac cttcaagttc accgaccagt ggagcctgac ggccggcctg cggtactacg
     1561 acttcagcga agaccgcacg atcaccttcg cgggggtctt cgccgacccc aacatcatca
     1621 cggacgtgcc gggcacgacc gagtccgacg gtttttcgcc gcgcgtgatc ctcgagttca
     1681 accccaccga agacgtcatg ctgacggccc aggtcgcacg aggtttccgg ctcggcggca
     1741 tcaacgaccc gttgaacgag ggactctgcg gatcgcaggc cgagatcgac acctacggtg
     1801 gctacccgat ctgggacgac gaaaagacca ccaactacga gctcggcacc aagacgcgct
     1861 gggccgacgg ccgggtgcag ttcaacgccg ccgcgttcta caccgatatc gacgggctgc
     1921 aggtcgtggt caacaccagt tgctcgtccc gcatcatcct gaacgcgcag gcccggtccg
     1981 tgggtggcga actcgagctc ttcgcccggc ccaccgacaa ctgggacttc ggcctgacgg
     2041 ccacgtacgt caatgccgag atcacggaaa cacagcgcgg gcccgacggc agtccgctcg
     2101 cgggtatccg ggagggcaat cgcctgccga cgtcgccgga actgcaggcg gcggcgagcg
     2161 caacc
//
LOCUS       DASPFR010000022         3300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1473294, whole genome shotgun sequence.
ACCESSION   DASPFR010000022 DASPFR010000000
VERSION     DASPFR010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473294"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VD737_00515"
     CDS             <1..396
                     /locus_tag="VD737_00515"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HXW09071.1"
                     /translation="SFHPEASAHVDRTLQLIRADGCRPGLVFNPATPLDVLEWVIDKV
                     DLVLVMSVNPGFGGQSFIDSALRKIERVRERIDASGRDIRLEVDGGIKVDNIATVAAA
                     GADTFVAGSAIFGAGDYAATIAAMRQAIG"
     gene            453..1226
                     /locus_tag="VD737_00520"
     CDS             453..1226
                     /locus_tag="VD737_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HXW09072.1"
                     /translation="MYLAYTRAVSPTIADCELTHLPRVAVEVGRAIVEHATYEQTLRR
                     LGAQVRRLAPLPRLPDAVFVEDTAVVLDELAIITRPGAESRRGERRSVAQALAAHRSI
                     EVIEEPATLDGGDVLVDGERVFVGRSSRTNDAALNQLATLLHPLGYRVIPVEFGGCLH
                     LKSAVTRIADGMLLLNPAWVQASVFSGTRCVTVDPQEPYAANALAFGGAVIHPLQFPR
                     TRARLEAEGLRVESVDTTELAKAEAGVTCCSLIVRIGRR"
     gene            complement(1279..2076)
                     /locus_tag="VD737_00525"
     CDS             complement(1279..2076)
                     /locus_tag="VD737_00525"
                     /inference="COORDINATES: protein motif:HMM:NF039649.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6090 family protein"
                     /protein_id="HXW09073.1"
                     /translation="MVLMRRFAAAVRTLRWGQVLFELALLVTGILIALAVNNWMDERR
                     EARAERQYLELLGRDLEQDLETLDEIRRFEEAQVAASALVFRGLRHGVAPADREAVAA
                     ALGQLTSRRTLRLRRAAYTNLLSTGELRLIRNTALRDRIVRLYENNERAQTIRDRNNQ
                     EYVDRMYMTYLLDQGLVAPRPARSLPSITGTDAAFAERVGIAPRVEDDPLWRLPRDAP
                     QLYVLANRAWYRGTVSQNALEQTRQVVKEIGEVRQAIERELAGRRWP"
     gene            complement(2334..2969)
                     /gene="crp"
                     /locus_tag="VD737_00530"
                     /pseudo
     CDS             complement(2334..2969)
                     /gene="crp"
                     /locus_tag="VD737_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016506431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
     gene            complement(3087..>3300)
                     /locus_tag="VD737_00535"
     CDS             complement(3087..>3300)
                     /locus_tag="VD737_00535"
                     /inference="COORDINATES: protein motif:HMM:NF015207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HXW09074.1"
                     /translation="EKQVCAHLDSHLAALPPEDERSRRIVARMKDDEDRHASDARASG
                     AAILPRPIRELMRRTARVMTRTAYRI"
BASE COUNT          459 a         1151 c         1152 g          538 t
ORIGIN      
        1 agcttccacc ccgaggcgag cgcgcacgtg gaccgcacgc tgcagctgat ccgcgccgac
       61 ggttgtcggc ccgggctggt gttcaacccg gcgacgccgc tggacgtgct ggagtgggtg
      121 atcgacaagg tcgacctcgt gctcgtgatg agcgtgaacc ccggcttcgg cggccagagc
      181 ttcatcgaca gcgcgctgcg caagatcgag cgcgtgcgcg agcgcatcga tgccagcggg
      241 cgcgacatcc gcctcgaggt cgacggcggc atcaaggtcg acaacatcgc gaccgtcgcc
      301 gcggccggcg ccgacacgtt cgtggcgggc tccgcgatct tcggcgccgg ggactacgcg
      361 gcgacgatcg ccgcgatgcg acaggcgatc ggctgatcgc cggcgcttgc ctcggcacga
      421 ctacgaccgc tcacgaccaa ccgtccgcgc cgatgtacct cgcctacacc cgcgcggtca
      481 gcccgacgat cgccgattgc gagctcacgc acctgccccg cgtcgcggtc gaagtcgggc
      541 gagcgatcgt cgagcacgcg acctacgagc agaccctgcg acggctgggc gcgcaggtac
      601 ggaggctggc cccgctgccg cgcctgccgg acgccgtgtt cgtggaggac acggccgtgg
      661 tgctcgacga gctcgcgatc atcacgcgcc cgggcgcgga gtcgcggcgc ggcgaacgcc
      721 gctcggtcgc gcaggcgctg gccgcgcatc gcagcatcga ggtcatagag gagccggcga
      781 cgctcgacgg cggcgacgtg ctggtcgacg gcgaacgcgt gttcgtcggc cgctcgagcc
      841 ggaccaacga cgccgcgctc aaccagcttg cgaccctgct gcatccgctc ggctatcgcg
      901 tgatcccggt cgagttcggg ggctgcctgc acctgaagtc ggccgtgacg cggatcgccg
      961 acggcatgct gctgctcaat cccgcctggg tgcaggcgag cgtgttctcg ggcacgcggt
     1021 gcgtgaccgt cgatccgcag gagccgtacg ccgccaacgc gctcgcgttc ggcggtgcgg
     1081 tgatccaccc gctgcagttc ccgcgcacgc gtgcgcgcct ggaggccgag gggctgcgcg
     1141 tcgagtcggt cgacacgacc gagctcgcca aggccgaagc cggagtgacc tgctgcagcc
     1201 tgatcgtgcg catcgggcgg cgctgaatca cctcgcccgc ctcgcgcgag aggtcgtcgc
     1261 gcagcgacgg gtgagccgtc agggccagcg acgcccggcc agttcgcgtt cgatcgcctg
     1321 ccggacctcg ccgatctcct tcacgacctg ccgcgtctgc tcgagcgcgt tctggctcac
     1381 cgtgccgcga taccaggccc ggttcgcgag cacgtagagc tgcggggcgt cccgcggcag
     1441 gcgccagagg gggtcgtctt cgacgcgcgg tgcgatgccg acccgctcgg cgaacgcggc
     1501 atccgttccc gtgatgctcg gcaatgagcg cgcgggccgc ggtgcgacca gtccctggtc
     1561 gagcaggtag gtcatgtaca tgcggtccac gtattcctgg ttgttgcggt cgcggatcgt
     1621 ctgcgcacgt tcgttgttct cgtagagccg cacgatgcgg tcgcgaagcg cggtgttgcg
     1681 gatgagccgc agctcgcccg tgctcagcag gttggtgtag gccgcgcggc gcaatcgcag
     1741 cgtccttcgg gaggtcagct ggccgagcgc ggcggccacg gcctcgcggt cggcgggcgc
     1801 cacgccgtga cgcaggccgc gaaagaccag ggcgctcgcc gcgacctggg cttcctcgaa
     1861 acggcggatc tcgtcgagcg tctcgaggtc ctgctcgagg tcgcgaccga gcagctccag
     1921 gtactggcgt tcggcgcgcg cctcgcggcg ctcgtccatc cagttgttga ccgcgagcgc
     1981 gatcaggatg ccggtcacga gcagtgccag ctcgaacagg acctggcccc accggagcgt
     2041 gcgcacggcc gcggcgaaac gtcgcatcag caccatgtct tccccctcgc cacgtccgat
     2101 cgcggaaagc aaacgggcgg gactctacgt caccgcccgt gggttgctct cccccgcgct
     2161 acgcgcgacc tctcccgtgc ccgggagagg ggaggacgtc aatcctcgtc gtcctcgtcg
     2221 tcgtcatcct cttcttcgtc gtgcgcggtc gtcgcgcgcg tggtccgcag cgcgaggccc
     2281 gacggaggtg cggcggcgcc ggccagcgcg gccgccttgc ccttgagccg cggacgcacg
     2341 ccgtagacca ccatcgtctt gccgcgagcg gtgagcaggc cctgctcctc gagcaccttc
     2401 agcacgcggc ccgccatttc gcgcgagcag ccgaccaggc gcgagagttc ctgccggctc
     2461 accttgatct gggtgccctc cgggtgcgtc atcgcatcgg gctcgttgca gaggtccatg
     2521 atcgcgtgcg cgacccggcc ggtgacgtcg acgaacgcga ggtcgccgag cttgcgattg
     2581 gtgcggtcga ggcggctcgc gagctgcgtc gcgagttcga acagcagcgt cgggctttcg
     2641 gcggcgatct gccggaagcg ggggtaggtc atctcggcga gttcgcactg cgagcgcgtt
     2701 cgcacccatg cgctgcgcgc gggctggtcg ttgaacagac ccatctcgcc gaagaactgg
     2761 cccttgttga ggtaggccag caccatctcg ttgccttcct cgtcctcgat caggacttcg
     2821 accgagccct tgatcacgta gtacaggacg tcggggacgt cgcccgcatg gatcagcacc
     2881 gtcttggacg gcacggtccg gatgcggcaa tagctcagga accggttgat cgccgggatg
     2941 tcgctcaggg gtcgtccacc gtgttccatg tctcccatcc catcctgttg gacgtttcgt
     3001 tcgtttatgt caacccaacg ctgcaggctt ttaatataaa ggtctgctgc gccgcaagtt
     3061 tttgctgccg cccagacgca accgaatcag atgcgatagg cggtccgggt catgacgcgc
     3121 gccgtgcggc gcatcagttc ccggatcggg cgcggcagga tcgcggcgcc gctcgcgcgc
     3181 gcatcgctcg cgtgccggtc ttcgtcatcc ttcatccggg ccacgatccg gcgcgagcgc
     3241 tcgtcctcgg gcggcagcgc ggccaggtgg gagtcgaggt gcgcgcagac ctgcttttcc
//
LOCUS       DASPFR010000023         4885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_862932, whole genome shotgun sequence.
ACCESSION   DASPFR010000023 DASPFR010000000
VERSION     DASPFR010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862932"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1810
                     /locus_tag="VD737_00540"
     CDS             98..1810
                     /locus_tag="VD737_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09075.1"
                     /translation="MTAEATAATPPVETPPPRGFKLLKAAVRNRKLGVMLALGFSAGL
                     PYALLLGTLYAWLGEAQVDLETMGVFSLIGLAYAFKFLWSPLVDRLVLPGLERLGRRR
                     SWLLPIQFALGGVFITLSSLDPGSMLGWFSLLAGIAAFASATQDIVIDAWRIDVADEV
                     ATLEILSAIYQLGYRFASLVGGALALIAAARIGWPAVYFWMGVAMLVVAGVTLLAPDT
                     PRRAASDGDDPLALHATVPLRARRVAIAVIGAAWTWALVTVVWFMVRSLGAAPGARPD
                     ATAFIKLYGPLIVVATVIVPALVAAILNGLARSRIPLATHTRPGVVDRTIAHAYGALL
                     MPLAEIVARMRWAAVLALGIILTYRLTDAIWGPFALPFYLQELHYSNDEVAIASKFFG
                     VGMTMAGIALGAVLFAVLGRMPTLVLGAAFAAGTNLLYADLAAGGAGLDVFGRTTGFY
                     ALAGMLGSDERLSRLLLAIAGENLAAGLAGAAFVAYLSGITSKQYSAVQYALLSSLTF
                     LVGSLGRGAIGEAIDVRGYAPVFYFTAALGSIAVVLCVFEWIRTRGGGATGSSGTPDA
                     PASA"
     gene            1827..2216
                     /locus_tag="VD737_00545"
     CDS             1827..2216
                     /locus_tag="VD737_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HXW09076.1"
                     /translation="MDAPGKTDEIDLARVICSLQELDDPGARGFTLGEGEWPLRGFVV
                     RRGTAVRAYVNHCPHAGFPLNWQPNVFLGPGGSHIRCIMHGALFEIETGICVAGPCMG
                     HGLQTLEIEVRDGYVLLDASVPLQDPN"
     gene            complement(2321..2695)
                     /locus_tag="VD737_00550"
     CDS             complement(2321..2695)
                     /locus_tag="VD737_00550"
                     /inference="COORDINATES: protein motif:HMM:NF042799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6763 family protein"
                     /protein_id="HXW09077.1"
                     /translation="MDASRSTTMANPAPVVGKWYARPGGDSFEIVAFDADDGTIEIQY
                     FDGTIEELDVEDWREEQILPAEPPEDWTGSVDVEPEDYENEFDTEPGNGLAGSPLDVI
                     DRGEVDGYSEFELPDEPDEKVG"
     gene            2769..3398
                     /locus_tag="VD737_00555"
     CDS             2769..3398
                     /locus_tag="VD737_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HXW09078.1"
                     /translation="MIDELVKFFVVLLVVVEPLSLLPVLAGLTQGADDSYRRHMSIKA
                     VGISATVCLVFALGGAWLLSLLGISVDAFKVGGGILLFLLAVEMVFARPSGVRSTTPR
                     EEAESHQRVDISVFPLAFPLIAGPGALATILLAFAGVPYGSPQFFAQVAVIFVVLGIT
                     LALMLVTGPVMRIVGVTGGAVLSRLLGVLLAALASQFVMDGIRGAMSVP"
     gene            complement(3417..4034)
                     /locus_tag="VD737_00560"
     CDS             complement(3417..4034)
                     /locus_tag="VD737_00560"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HXW09079.1"
                     /translation="MVNPKDGLLAHLGGDARALCRRAWWVFLVSGLASVAFAVLAFAQ
                     PVTAWMVVALFFAAAILVDGVVNVIGAIQHREKDGWWLMLLIGVLGALVGGYALTHPR
                     LGMVAFVYLVAFQAMLLGVLVVALGYKVRAATQREWILYLTGALSILFGLLILANPAA
                     GGISVILLIAGWALVTGLLRILFAFRIRASVQETDAMPPASPPAA"
     gene            4197..4865
                     /gene="trmB"
                     /locus_tag="VD737_00565"
     CDS             4197..4865
                     /gene="trmB"
                     /locus_tag="VD737_00565"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HXW09080.1"
                     /translation="MRSFVVRAGRMTQAQQRAWRELWPTWGLEPGEAALDLAATYGRA
                     APVTLEIGFGNGESLVALAASHPERNFLGLEVHPPGVGHALLSCEAARLTNVRVLCQD
                     AVEVLQRRLADASLDEVLLYFPDPWPKKRHHKRRIVQPAFVELVAQRLRPGGVLRMAT
                     DWQPYAEHMLATVGACALLRNESPSGDYVERPASRPVTRFERRGQRLGHDVWDLAFRK
                     WGHS"
BASE COUNT          663 a         1671 c         1700 g          851 t
ORIGIN      
        1 tgctgagccc cgggctcaag ggcagcgtcc gcagcgtcga gatcttcaag gcgcagcggt
       61 tttccgacca tgcgccgctc accgtgacgt acgacctgtg acggccgagg cgacggcggc
      121 gacaccgccc gtcgagacgc cgccgccgcg cggcttcaag ctgctcaagg ccgcggtccg
      181 caaccgcaag ctcggcgtga tgctggcgct cggcttttcc gcagggctac cctatgcgct
      241 gctgctcgga acgctctacg cctggctcgg agaagcgcag gtcgacctcg agaccatggg
      301 cgtgttctcg ctgatcggcc tcgcctacgc gttcaagttc ctgtggtcgc cactggtgga
      361 ccggctcgtg ctgccgggac tcgagcggct cggccggcgg cgctcgtggc tgctgccgat
      421 ccagttcgcg ctcggcggcg tgttcatcac gctgtcctcg ctcgatcccg gctcgatgct
      481 gggctggttc tcgctgctcg ccggcatcgc cgcgtttgcg tcggcgacgc aggacatcgt
      541 gatcgacgcg tggcgcatcg acgtggccga cgaggtggcc acgctcgaga tcctctcggc
      601 gatctaccag ctcggttatc gctttgcctc gctggtggga ggcgcgctcg cgctgatcgc
      661 ggcggcgcga atcggctggc ccgcggtgta cttctggatg ggcgtcgcga tgctcgtggt
      721 ggctggcgtc acgttgctcg ccccggacac gccgcgccgc gccgcgagcg acggggacga
      781 cccgctcgcg cttcatgcga ccgtgccgct gcgcgcgcga agggtggcca tcgccgtgat
      841 cggcgcggcg tggacgtggg cgctcgtgac ggtggtgtgg ttcatggtga ggtcgctcgg
      901 cgcggcgccc ggcgcgcgac ccgatgcgac cgcgttcatc aagctctacg gacccctgat
      961 cgtcgtggcg acggtcatcg tgccggcgct ggtggccgcg atcctgaacg gactcgcccg
     1021 ctcacggata cccctcgcga cgcacacacg ccccggcgtc gtcgatcgca cgatcgcgca
     1081 tgcctatggc gcgctcctga tgccgcttgc cgagatcgtc gcgcggatgc ggtgggccgc
     1141 cgtgctcgcg ctcggcatca tcctgaccta ccgcctcacc gacgcgatct ggggcccgtt
     1201 tgcgttgccg ttctacctgc aggagctgca ctacagcaac gacgaggtcg cgatcgcgag
     1261 caagttcttc ggcgtcggca tgacgatggc cgggattgcg ctgggcgccg tgctgttcgc
     1321 ggtgctcggt cgcatgccga cgctcgtgct cggcgcggcc ttcgcagccg gaaccaacct
     1381 cctgtacgcc gacctcgcgg caggcggcgc cggcctcgac gtgttcgggc gcacgaccgg
     1441 cttctacgcg ctcgctggca tgctgggctc ggacgagcgc ctgtcgcgcc tgctgctggc
     1501 catcgcgggc gagaacctcg ccgccggcct ggccggcgcg gcgttcgtcg cgtacctctc
     1561 cggcatcacg tcgaagcagt acagcgccgt gcagtatgcg ttgctgtcct cgctcacgtt
     1621 cctggtcggg tcgctcggtc gaggtgcgat cggcgaggcg atcgacgtgc gcgggtacgc
     1681 gcccgtcttc tacttcacgg ccgcgttggg cagcatcgcc gtcgtgcttt gcgtcttcga
     1741 gtggatccgg acgcgcggtg gcggagcgac ggggtcctcg gggactcccg acgctccggc
     1801 gtcggcttga ggtgacggtc gcgtgcatgg acgctcccgg caagacggat gaaatcgacc
     1861 tggcgcgggt gatctgctcg ctgcaggaac tcgacgaccc gggtgctcgc ggcttcacgc
     1921 tgggcgaagg cgagtggccg ctgcgtggct tcgtcgtgcg tcgcggcacg gcggtgcgcg
     1981 cctacgtcaa tcactgcccg cacgccgggt ttccgctgaa ctggcagccg aacgttttcc
     2041 tcgggccggg cggctcgcac atccgctgca tcatgcacgg cgcgctgttc gagatcgaaa
     2101 cgggcatctg cgtcgccggc ccgtgcatgg gccacggact gcagacgctc gaaatcgagg
     2161 tacgcgacgg ctacgtgctg ctcgacgcga gcgttccgct gcaggatccg aattgacctc
     2221 ccgctccggt gacgacggtc gcaggaaagc gcgggtgagg gccactctgc acctctccca
     2281 ggacggtggg agaggtcgcg cgcagcgcgc gtgagggcaa ctagccgacc ttttcgtccg
     2341 gctcgtcggg cagttcgaac tcgctgtagc cgtcgacctc gccgcgatcg atcacgtcga
     2401 gcggactgcc ggcgagcccg ttgccgggct cggtgtcgaa ttcgttctcg tagtcttcgg
     2461 gctcaacgtc gacggagccg gtccagtctt ccggcggttc agccggcagg atctgctcct
     2521 cgcgccagtc ctccacgtcg agttcctcga tcgtgccatc gaaatactgg atctcgatcg
     2581 tgccgtcgtc ggcgtcgaag gcgacgatct cgaacgagtc gccgccaggc cgtgcgtacc
     2641 acttgcctac gaccggtgcg gggttggcca tcgtcgttga cctcgaggcg tccatgcgct
     2701 tggtatcctt gggtctgttg tagtcctgcg cggctcggga ttcaagtcgc gaaacacacg
     2761 gccccatcat gatcgacgaa ctggtcaaat tcttcgtcgt cctgctcgtc gtcgtggagc
     2821 ctttgtcgct cctgcctgtg cttgcgggac tgacgcaggg cgcggacgat tcctaccgtc
     2881 ggcacatgtc gatcaaggcg gtcggcatct cggcgacggt ctgtctcgtc ttcgcgctcg
     2941 gcggggcgtg gctgctgagc ctgctcggca tcagcgtcga cgcgttcaag gtcggcggcg
     3001 ggatcctgct gttcctgctg gcggtcgaga tggtgttcgc gcgcccctcc ggtgtccgct
     3061 cgacgacgcc acgcgaggaa gccgagtcgc atcagcgcgt cgacatctcc gtcttcccgc
     3121 tcgcgtttcc gttgatcgcc gggcccggtg cgctcgcgac gatcctgctc gcgttcgccg
     3181 gcgtgcccta cggctcgccg cagttcttcg cgcaggtcgc ggtgatcttc gtcgtgctcg
     3241 gcatcacgct tgcgctgatg ctggtcaccg gtccggtgat gcggatcgtt ggcgtcacgg
     3301 gcggcgcggt gctcagtcgt ctgctgggcg tgctgctggc ggcactggcc tcgcagttcg
     3361 tcatggacgg catccgcggc gcgatgtcgg tgccgtgaac ggccggtcgg agtcgcctac
     3421 gccgcaggag gtgaagcggg cggcatcgcg tccgtttcct gcacgctcgc gcggatgcgg
     3481 aatgcgaaca ggatgcgcaa cagccccgtc acgagcgccc agcccgcaat gagcaggatc
     3541 acggagattc cgcccgccgc cgggttcgcc aggatcagca ggccgaacag gatcgagagc
     3601 gcgccggtca ggtagaggat ccactcgcgc tgcgtcgccg cccgcacctt gtaaccgagc
     3661 gcgaccacga gcacgccgag cagcatggcc tggaaggcga ccaggtagac gaacgccacc
     3721 atccccagcc gcggatgggt cagtgcgtaa ccgccgacca gcgcgccgag caccccgatc
     3781 agcagcatca gccaccagcc gtccttttcg cgatgctgga ttgcgccgat cacgttgacg
     3841 acgccgtcga cgaggatcgc ggcggcgaag aacagtgcga cgaccatcca ggccgtaacc
     3901 ggctgcgcga acgcgagcac cgcgaaggcg acggacgcga ggccgctgac gagaaacacc
     3961 caccaggcgc gtcggcacag cgcgcgggcg tcgccgccca ggtgggcgag cagtccgtcc
     4021 ttcgggttga ccatgcgcga tcctcccaac gccgcggcgg ggccgtatga tgcgcgaccc
     4081 ttcttcgctg tgactgtagt ccgttgaacc cgacgaaacc agacgtggac ggccgtcacg
     4141 gcgaagacgc ttcgcccgcc gcggacgcgt cgtctgcgca ccgcactccg cgcgcgatcc
     4201 gcagtttcgt cgtgcgcgcc ggccgcatga cgcaggcgca gcagcgcgcc tggcgcgaac
     4261 tgtggccgac gtggggcctc gagcccggtg aggccgcgct cgacctcgcc gcgacctacg
     4321 gtcgagcggc gcccgtcacg ctggagatcg gcttcgggaa cggcgagtcg ctggtcgcgc
     4381 tggcggcgag ccatcccgag cggaatttcc tggggctcga agtgcaccca ccaggtgtcg
     4441 gacacgcgtt gctgagttgc gaggcggcgc gcctcacgaa cgtccgcgtg ctgtgccagg
     4501 acgccgtcga ggtcctgcag cggaggttgg ccgacgcgtc actggacgaa gtgctgctgt
     4561 acttcccgga tccgtggccg aagaagcgcc atcacaagcg caggatcgtg cagccggcgt
     4621 tcgtcgagct cgtcgcgcag cgcctgcgtc ccggcggcgt gctgcgcatg gcgaccgact
     4681 ggcagccgta cgcggagcac atgctcgcga ccgtcggcgc ctgcgcgttg ctgcgcaacg
     4741 agtcgccgag cggcgactac gtcgagcgtc ccgccagccg gccggtcacg cggttcgagc
     4801 ggcgcggcca gcggctcggc cacgacgtct gggacctcgc cttccggaaa tggggacatt
     4861 cctgaatttc ggataaatgg ggaca
//
LOCUS       DASPFR010000024        10572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1595807, whole genome shotgun sequence.
ACCESSION   DASPFR010000024 DASPFR010000000
VERSION     DASPFR010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10572
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595807"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1514
                     /locus_tag="VD737_00570"
     CDS             <1..1514
                     /locus_tag="VD737_00570"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HXW09081.1"
                     /translation="SPAVAAATLLFSGVAAHSAEPVQGPTSSKPSYVQPTATGWTSTS
                     LITVGDGADQNGYRMVGLPDGLGAIGGAWSAEQRKYLRPDLYLTVFMNHEVRTEWGVP
                     RAHGQKGAFVSQWTIELGSLRVLRGEDLIRRVMTWNYSTGSYQETTGSTIFDRFCSGD
                     LPKPTAFRHAASGRGTPVRLYLNAEEADVNGRAFAHVVSGGQKGTSYELPYLGKAKWE
                     NALAHPAAGTQTVVMMLEDRSPGQLYLYVGDKQSTGTIIQRAGLQGGKLYAVKVIDGG
                     PNYGGGAVRIENAGAVNGRFALVDVSAYALASSDVLDTRSDEAGVTEFARPEDGQWDP
                     TNPRVFYFNVTGSTTQSARLYRLRFDGTTWPAGGTIELVLDSTALVGTDGQGARGFDN
                     LTVSPNGTVMVQEDGAADAYLSKTWKVKPANPSAAVQVVRASPTFFSTSSPSYLTSSE
                     EMSGIIDVTAVVKPAPWFQTRRRYYLATIMDHSMEPDPELVQGGQLYLLSGPP"
     gene            complement(1528..2814)
                     /locus_tag="VD737_00575"
     CDS             complement(1528..2814)
                     /locus_tag="VD737_00575"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXW09082.1"
                     /translation="MDTQMLVLGSALALTGLAISALVRPNFLVRDNALWSACSWFAGE
                     FAPWLGGGALLALIALANTTQAFAGERGRLAALLLVASAAGLLTVANRTRRTRSVLEG
                     ALRDTLGPGYLSDVPPTRMPALIEEVPRRHYFRLLPRRPPAVELVANLPYPGGDARNV
                     LDIYRPAAGCHDAPVVLQIHGGGWTMGNKHQHAMPLVHHLASLGWIVVTPNYRLCPAA
                     RMPGPLVDCKAAMAWIRGHIAGYGGDPSFVAVTGGGAGAHLAALLALTFDEPELQPGF
                     EHVDTRPAACVPLHGIYDLADRRHRHPGRAARLKWLGDNVMACALERDALAWDMASPR
                     ALLREDAPPFFVLHGTHDALSPVGEAREFARELRLASTQPVVYAELDGAQHGWDTLCS
                     PRALHTVRAVTRFLEWCAARHRAQGSRLHDANRARS"
     gene            complement(2880..4166)
                     /locus_tag="VD737_00580"
     CDS             complement(2880..4166)
                     /locus_tag="VD737_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXW09083.1"
                     /translation="MRACRPLLPWLLLACACSPAANAARLTEPERRIVAAVDARVAAF
                     EPLLAETVDVASATENPAGVRAVGEIYAREYRRLGFSTEWAELPAEMNRAGHFVARHE
                     GRAAAPRLLLIGHLDTVLQGEPFRREGDRAYGSGTADMKGGNLIALEAVRALATAGQL
                     DGMNVTVVYTGDEEDPAPPLSVARQALRQAAQSAAIALGFEGASEGKAVTARRGIGSW
                     RLSVSGIQGHSSGIFGERGYGAIFEAARILDRFRTELREANLTFNPSVILGGTVVTYT
                     SADTRGTALGKTNVIPRDVQVEGDIRYLGPAQLEAAERRMRAIVAAGLPGTSATLEVT
                     AEYPSMAPNPGSERVLGVFDGVSRDLGAGPIVAQPPMERGAGDISFVCEDGRLACLDG
                     LGAIGDEDHAPGEYLELDALPMQVKRAALLMYRLTR"
     gene            complement(4287..4868)
                     /locus_tag="VD737_00585"
     CDS             complement(4287..4868)
                     /locus_tag="VD737_00585"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HXW09084.1"
                     /translation="MGWLIAGLLLFLGSHSIGIAAPAWRNATMARLGPRAWKALIGLV
                     SIAGFLMIIHGYGVGRVDAAVLYAPPPAFRHVAALLMLPVFPLLLATYLPGRIKSAVK
                     HPMLTATKAWAVAHLLVNGSVADVLLFGGFLVWAVADRIAVGRRPVERTLPGAPPSRW
                     NDVVAIVGGLVLYALFVGGLHQRWFGVAPLLPH"
     gene            complement(4878..5852)
                     /locus_tag="VD737_00590"
     CDS             complement(4878..5852)
                     /locus_tag="VD737_00590"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXW09085.1"
                     /translation="MDQLTAMRVFVRVAERGSFSLAADELELSRPAASAHVAALEKHL
                     GARLLHRTTRKVSLTAEGADFLDRARRILGELRDAEDALRDTRSKPQGRLRVDVPVAF
                     GENLLLPALPEFAKRYPLIELEVRFNDRVVDLVGEAVDVALRVGTVQQAGLVVRRISG
                     INLVTCASPGYLETHGEPRTPDDLRQHRLLGVTQASGAPPEWTFPAPYTPRRLGLKFA
                     MLFNTALGPVVSAAGGLGITHTADLLIAEHVARGSLKLILEDYIVAGPPMSLVYPSGG
                     HQAAKVRVFSDFAADLLLRWHEQVRRRLQGPPAHRHGRPDTLRGETDH"
     gene            5959..6084
                     /locus_tag="VD737_00595"
     CDS             5959..6084
                     /locus_tag="VD737_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09086.1"
                     /translation="MFQRKSHETFFPVAIGAALAVTVTVVIAALMEAVAGMESFL"
     gene            6466..7923
                     /locus_tag="VD737_00600"
     CDS             6466..7923
                     /locus_tag="VD737_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HXW09087.1"
                     /translation="MIPPDTPESLDPPDWDALRRLAHRAVDDGLNYLQRVRERPVWQP
                     IPERVTAQLRGPAPREPQGADRAYADFSEYVLPYPMGNIHPRFWSWFMGNGTAFGAVA
                     DFLAAVMNPNMGGGNHVANHVEAQVLDWSKEMIGFPAEASGLLVSGGSVANFVGLAVA
                     RNARSGVDVRELGVAAIPKPLVTYASVEVHSCVQTAIESLGLGARSLRKVPVDAAYAI
                     DVAALERMIAEDRSAGRQPFCVIGNAATINTGATDDLEQLATVCEREHLWFHVDGAIG
                     SLLALAPKHRHLVRGIERADSVTLDFHKWLHVPFEAACVLVRDRQAHREAFALTPEYL
                     EKTERGLASGAHWFSEYGLQLSRGFRALKVWMTIKEHGLDRFGRLIDRNIEQAQELAA
                     LIAAEPQLQLVAPVPTNIVCFRFDPGGRDTAALNALNQELLIRLHESGIAAPSYTTLG
                     EVYCLRAAITNFRTTSEDLRVLVRAVVEIGRSLVN"
     gene            complement(7916..9031)
                     /locus_tag="VD737_00605"
     CDS             complement(7916..9031)
                     /locus_tag="VD737_00605"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXW09088.1"
                     /translation="MRRVVNAAMAVLAALVAYLVLWPVPIEPVAWSSQKFAGYAGPHA
                     RNDRLAKLRLVSIAPHVGPEHIVFGPDGLLYTGVRSGAVLRMQPDGTGVETYADTKGR
                     PLGLAFEATGRLVIADAVRGLLAVQPGGAVVVLADSVALEPIRYADAVVVASDGKILF
                     TDASMRFSPHELGTFDAALLDILEHSCTGRVLEYDPAAPATRIVANGLCFPNGIALTA
                     DERSLLVAETGTYRILELPRNANLLDVGAALQARQPDVRVLLDDLPGFPDNLTRGGDG
                     RYWVGLTKPRSDTIDGMAAKPWLRSLTLRLPRALWPVPPAYGHVIAFDDSGRVVADLQ
                     DPEGRLPETSGATEHQGQLYVQSLHAEALGMLPLPVN"
     gene            complement(9041..9913)
                     /locus_tag="VD737_00610"
     CDS             complement(9041..9913)
                     /locus_tag="VD737_00610"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HXW09089.1"
                     /translation="MASSSKIVVTCALTGVLTDPAQHHVPVTPEQMAREARAACDAGA
                     SVVHVHFRQQAPGRGHLPSWDPQVALEIAQAIREACPGVVFNQSTGVIGPDISGPVAC
                     MRAIRPEIAAANAGSLNYLKVRSDGRWAWPPLLFDNPVEKVAAFLQAMQETGAMPEFE
                     CFDVGIVRSVEMYLRTGLYRGHADVNFVMGVDSGMPADPALLPLLIGLLPQAATWQVT
                     AIGRENVWPLHRRTAELGGNLRTGLEDTFYLPDGRKARGNGELIEALVALAREAGREC
                     ATCAEARTLLHLAA"
     gene            complement(9913..>10572)
                     /locus_tag="VD737_00615"
     CDS             complement(9913..>10572)
                     /locus_tag="VD737_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HXW09090.1"
                     /translation="ASRQPVPDARTWSLAAWLSVTAAPESLATAEPWRHWGTGRPLPQ
                     PWRLRWNASQSAAAAGPCERQGRVVLEGPRALVVVAGQDQGVVEGPAAAPRRHARVTI
                     DGEPVEYRHAWDGATLWLHVAHGDFAFECLRRASARAAGATGDAVREVRSSMNGRVVD
                     VAAAAGALVAKGDRLVVLEAMKMEHEIRATRDAVVAEVLVRAGDQVVPGQVLVRYAGE
                     P"
BASE COUNT         1642 a         3732 c         3693 g         1505 t
ORIGIN      
        1 ccagcccggc cgtcgccgcc gcgacgctcc ttttcagcgg cgtggccgcg cactccgcgg
       61 agcccgtgca ggggccgacc agttcgaagc cgtcctacgt gcagccgacc gcgaccgggt
      121 ggacctcgac gtcgctgatc accgtcggcg acggcgccga ccagaacggg taccgcatgg
      181 tcggcctgcc ggacgggctc ggcgcgatcg gtggcgcgtg gtcggcggag cagcgcaagt
      241 acctgcgtcc ggacctctac ctgacggtct tcatgaatca cgaggtccgc accgaatggg
      301 gcgtgccgcg cgcacacggc cagaagggcg cgttcgtctc gcaatggacg atcgagctcg
      361 ggtcgttgcg cgtgctgcgc ggcgaggacc tgatccgccg cgtcatgacg tggaactatt
      421 cgaccggcag ctaccaggag acgaccggct cgacgatctt cgatcgcttc tgctcggggg
      481 acctgcccaa gccgaccgca ttcagacatg cggcgagcgg acgcgggacg ccggtgcgtc
      541 tgtacctcaa cgcggaggag gctgacgtca acggtcgcgc gttcgcgcac gtcgtctccg
      601 gcggacagaa gggcacatcg tacgaactgc cgtacctcgg caaggccaag tgggaaaacg
      661 cgctggcgca cccggccgcc ggcacgcaga cggtggtcat gatgctcgag gatcgctcgc
      721 ccgggcagtt gtacctgtac gtcggcgaca agcagtcgac cggtacgatc atccagcgcg
      781 cgggcctgca gggcggcaag ctgtacgcgg tcaaggttat cgatggcggg cccaactatg
      841 gcggtggcgc ggtgcgcatc gagaacgccg gcgcggtcaa cggccgcttc gcgctcgtcg
      901 acgtgtccgc ctacgcgctc gcgtcgagcg acgtgctcga cacacggagc gacgaggccg
      961 gggtgaccga gttcgcgcgc cccgaggacg ggcagtggga cccgaccaac ccgcgagtct
     1021 tctacttcaa cgtgacgggc tcgacgacgc agtcggcacg gctgtaccgg ctgcgcttcg
     1081 acggcaccac gtggccggcc ggcggcacga tcgaactggt gctcgactcg accgcgctgg
     1141 tcggcacgga cgggcagggt gcgcgcggtt tcgacaacct cacggtcagc ccgaacggca
     1201 ccgtgatggt ccaggaggac ggtgccgcgg acgcgtacct gagcaagacg tggaaggtga
     1261 agcccgcgaa tccgtccgca gccgtgcagg tggtgcgcgc gagcccgacg ttcttctcga
     1321 cttccagccc gagttacctg acctcgtccg aggagatgtc cggaatcatc gatgtgacgg
     1381 cggtggtgaa gccggccccc tggttccaga cccgccggcg ctactatctc gccaccatca
     1441 tggaccattc gatggagccg gacccggagc tcgtgcaggg cggacagctc tacctgctct
     1501 ccggtccgcc ctgatgcctt cgcgccccta ggagcgcgcg cggttggcgt cgtgcagccg
     1561 cgagccctgc gcgcggtgcc gcgccgcgca ccattcgagg aatcgcgtga ccgcccgcac
     1621 cgtgtgcagc gcgcgcggtg agcagagggt gtcccagccg tgctgggcgc cgtcgagctc
     1681 cgcgtagacg acgggctgtg tagaggcgag acgcagttcg cgcgcgaact cccgcgcctc
     1741 gcccacgggg ctcagcgcgt cgtgcgtgcc gtgcagcacg aagaacggcg gcgcgtcctc
     1801 gcgcagcagc gcacggggcg acgccatgtc ccaggcgagc gcgtcgcgct cgagcgcaca
     1861 tgccatcacg ttgtcgccga gccacttcag gcgcgcggcg cgccccgggt ggcgatgcct
     1921 gcggtcggcg aggtcgtaga tgccgtgcag cggcacgcag gcggcgggcc gcgtgtcgac
     1981 gtgctcgaag cccggctgca gctcgggttc atcgaacgtg agcgccagca gtgcggcgag
     2041 gtgggcgccg gcgccgccac ccgtcaccgc gacgaacgac gggtcgccgc cataaccggc
     2101 gatgtggccg cgtatccagg ccatcgcggc cttgcaatcg accagcgggc ccggcatccg
     2161 cgcggccggg cagagccggt agttcggcgt gacgacgatc cagccgagcg acgcgagatg
     2221 gtgcacgagc ggcatcgcgt gctggtgctt gttgcccatg gtccacccgc cgccgtggat
     2281 ctgcagcacg accggcgcgt cgtggcaccc cgcagcgggc cgatagatgt cgagcacgtt
     2341 gcgcgcgtca ccgccgggat acggcaggtt ggcgaccagc tccaccgcgg gcgggcgccg
     2401 aggcaacaga cggaagtagt gccggcgcgg gacctcctcg atcagcgccg gcatccgcgt
     2461 cgggggcacg tcgctcaggt agcctgggcc gagcgtgtcg cgcagggccc cctcgaggac
     2521 cgatcgtgtt ctccgggtgc ggttcgccac ggtcagcagc ccggccgcag aggcgacgag
     2581 caggagcgcg gccagccgtc cgcgctcccc ggcgaaggcc tgcgtcgtat ttgcgagcgc
     2641 gatcagtgcg agcagtgcgc cgccgccgag ccacggcgcg aattcaccgg cgaaccagct
     2701 gcaggcggac cagagcgcgt tgtcgcgcac caggaagttg gggcgcacca gcgccgaaat
     2761 cgccagtccc gtcagcgcga gagctgaccc cagcacgagc atctgcgtgt ccatgcgcac
     2821 ctcgcgggcc tccgtgcccg cgcctcacca gtcctgtgtc ctgcgtcaat gcgcccgcat
     2881 caacgcgtca acctgtacat cagcagcgcc gcgcgcttga cctgcatagg cagcgcgtcc
     2941 agctcgaggt actcgcccgg cgcgtgatcc tcatcgccga tcgcgcccag cccgtcgaga
     3001 caggcgaggc gaccgtcttc acagacgaac gaaatgtccc cggcgccgcg ttccatcggc
     3061 ggctgcgcga cgatcggccc ggcccccagg tcgcgactca cgccgtcgaa cacgccgagg
     3121 acccgctcgc tgcccggatt cggcgccatc gacgggtatt cggcggtcac ctcgagcgtg
     3181 gcgctggtgc cgggcaggcc ggccgcgacg atcgcgcgca tgcggcgctc ggcggcttcg
     3241 agctgcgccg gcccgaggta gcggatgtcg ccctcgacct gcacgtcgcg cgggatcacg
     3301 ttggtcttgc cgagcgccgt gccgcgcgtg tcggctgacg tgtaggtgac gaccgtgccg
     3361 cccaggatca ccgaaggatt gaacgtgagg ttggcctcgc ggagttccgt gcgaaagcgg
     3421 tcgaggatgc gcgccgcctc gaagatggcg ccgtagccgc gctcgccgaa gatccccgac
     3481 gagtgaccct ggatgccgga gacggacagg cgccaggacc cgatgccgcg tcgcgcagtg
     3541 acggccttgc cctcggaggc gccttcgaag ccgagcgcga tcgccgcgga ctgcgcggcc
     3601 tggcgcaggg cctggcgtgc caccgacagc ggcggcgcgg ggtcttcctc gtcgcccgtg
     3661 tagacgaccg tcacgttcat cccgtcgagc tggccggcgg tcgccagtgc acgcacggcc
     3721 tcgagcgcga tcaggttgcc gcccttcatg tcggcggtgc cgctgccgta ggctcgatcg
     3781 ccttcgcggc gaaacggctc gccctgcagg accgtgtcga ggtggccgat cagcagcagg
     3841 cgcggcgcag cggcacgacc ttcgtggcgg gcgacgaagt gcccggcacg attcatttcc
     3901 gccggcagct cggcccactc cgtgctgaat ccgagacggc gatactcgcg cgcgtagatc
     3961 tctcccacgg cccgcacccc ggcgggattc tccgtcgcgc tggcaacgtc gaccgtctcc
     4021 gcgagcagcg gctcgaacgc cgcgacccgt gcgtcgaccg cggcgacgat gcgacgctcg
     4081 ggctcggtga gccgcgcggc attcgcggcc ggcgagcagg cgcacgcgag cagcagccac
     4141 ggcagcaacg ggcggcaggc acgcatcgat cgcacggcta cctcaagcct tgattcagtg
     4201 tccgaggcta cacaaggcgg gtgacgcgcg aaaaccggcg gcgcggactc gcgcctgccg
     4261 cgaaggctga cgtcactgtc ggtcgctcag tgcggcagta ggggggcgac gccgaaccat
     4321 cgctggtgca gccctccgac gaacagcgcg tagagcacga ggccgccgac gatggccacg
     4381 acgtcgttcc accgcgacgg cggcgcgccg ggcagcgtgc gttcgaccgg gcgcctgccg
     4441 acggcgatgc ggtcggcgac cgcccagacc aggaagccgc cgaacagcag cacgtcggcc
     4501 acgctgccgt tgaccagcag gtgcgcgact gcccacgcct tggttgccgt gagcatgggg
     4561 tgcttgacgg cgctcttgat gcgacccggc aggtaggtgg cgagcagcag cgggaatacc
     4621 ggcagcatca gcagcgcggc gacgtgccga aatgccggcg gcggcgcgta gaggacggct
     4681 gcgtcgacgc gcccgacgcc gtagccgtga atgatcatca ggaagccggc gatcgagacc
     4741 agcccgatca gtgccttcca cgcgcgcggc ccgagccggg ccatggtcgc gttccgccag
     4801 gcgggcgcgg cgataccgat cgaatggctg ccgaggaaca gcagcagtcc tgcgatgagc
     4861 cagcccatcg gtcgtcctca gtggtccgtt tcgccgcgca gtgtatccgg acgtccgtga
     4921 cggtgcgcgg gcggaccctg gaggcgacga cgtacctgct cgtgccagcg cagcagcagg
     4981 tcggccgcaa agtccgagaa cacgcgcacc ttggcggcct ggtggccgcc cgacgggtac
     5041 accagcgaca tcggtggtcc ggcgacgatg taatcctcga ggatcagctt gagcgagcct
     5101 cgcgcgacgt gctcggcgat cagcaggtcg gccgtgtgcg tgatgccgag cccgcccgcg
     5161 gccgacacga ccgggccgag tgccgtgttg aacagcatcg cgaatttgag gccgaggcgt
     5221 cggggcgtgt atggcgccgg gaaggtccac tccggcggcg cgccggaggc ttgcgtgacg
     5281 ccgagcagcc ggtgctggcg caggtcgtcg ggcgtacgcg gctcgccatg cgtctccagg
     5341 tagccggggg aggcgcaggt caccaggttg atgccggaaa tgcgacgcac cacgagccct
     5401 gcctgctgca cggttcccac gcgcagcgcg acgtcgacgg cctcgccgac caggtcgacg
     5461 acgcggtcgt tgaagcgcac ctcgagctcg atcagcggat aacgcttggc gaattcgggc
     5521 agcgccggca gcagcaggtt ttcgccgaac gcgaccggga cgtcgacgcg cagccggcct
     5581 tgcggcttcg agcgggtgtc gcgcagggcg tcctcggcgt cccgcagctc gccgaggatg
     5641 cgtcgcgccc ggtcgaggaa atccgcgccc tccgcggtaa gcgacacctt gcgggtcgtg
     5701 cgatgaagca ggcgggcgcc gaggtgcttt tcgagagcgg cgacgtgggc gctcgccgcc
     5761 gggcgcgaca actcgagctc gtcggccgcc agtgagaacg agccgcgctc ggccacccgc
     5821 acgaagacgc gcatcgcggt gagctggtcc atcgcccgat tgttatttaa aacagaatag
     5881 agtgcaaagg ttactgcgat ttatcagaac aaacgatcgg cgtagctttc ctcccgcacg
     5941 cacacaggag gatccaaaat gttccagcgc aaatcgcacg aaacgttctt cccggtcgcc
     6001 attggcgctg ccctggccgt cacggtcacg gtcgtgatcg cggcgctcat ggaggccgtg
     6061 gcgggaatgg agagctttct ttgagctcga cgtcgagccg ggcggcacct tttcagtccg
     6121 tccggctcga agtttttatt ataataatca acggtttgat caattaatgc tgcaacgcag
     6181 catgaaataa gctatagttc tccacgatcg cccggtcgtg atcggctgcc gagccgcccg
     6241 acccccctcc tgcagcacgg agccggtttc gacctcgcga tcgctggagg tcgcatgtct
     6301 caatcgaagt atcgtgccat ggtgctgtcc tggctcgcat tggccaccgc ggcgaccttt
     6361 gccgccatcg cccgcgcact gcaggccggc cagttcgtgt actgagtcga gtcccgtcct
     6421 tcccccatag taggctgagc cggaagagcc caccccggag ccgccttgat ccccccggac
     6481 acgcccgaat cgctcgaccc gcctgactgg gacgccctgc ggcggctggc gcaccgcgcc
     6541 gtcgacgacg gcctgaacta cctccagcgc gtccgcgagc gccccgtctg gcagcccata
     6601 ccggaacgcg tcaccgccca gttgcgcggc ccggcgccgc gcgaaccgca aggtgccgat
     6661 cgcgcctatg ccgacttcag cgagtacgtg ctgccgtacc cgatgggcaa catccacccg
     6721 cgcttctggt cctggttcat gggcaacggc accgcgttcg gcgccgtcgc ggatttcctc
     6781 gcggccgtca tgaacccgaa catgggcggc ggcaaccacg tggcgaatca cgtcgaggcg
     6841 caggtgctcg actggtcgaa ggaaatgatc ggctttcccg cggaggcgag cggtctgctg
     6901 gtcagtggcg gctcggtcgc gaacttcgtc gggctcgcgg tcgcgcgcaa tgcccgcagc
     6961 ggcgtcgacg tgcgcgaact cggcgtcgca gcgatcccga agccgctcgt cacgtacgcc
     7021 tcggtcgagg tccacagctg cgtccagaca gcaatcgagt cgctgggact cggcgcgcgc
     7081 tcgctacgca aggtgcccgt cgatgccgcc tacgcgatcg acgtcgcggc gctggaacgg
     7141 atgattgccg aggaccggtc ggcgggacgc cagccgttct gcgtgatcgg caacgccgcg
     7201 accatcaaca ccggcgccac agacgacctg gagcagctcg cgaccgtctg cgagcgtgag
     7261 cacctctggt tccacgtcga tggtgcgatc ggctcgctgc tcgcgctggc gccgaagcac
     7321 cgacacctcg tccgcggcat cgagcgcgcc gactccgtga cgctcgactt ccacaagtgg
     7381 ctgcacgtgc cgttcgaggc cgcctgcgtg ctcgtgcgcg accgccaggc gcatcgggag
     7441 gctttcgcgc tgacgccgga gtacctcgag aagaccgagc gcggcctcgc gagcggcgcg
     7501 cactggttca gcgaatacgg cctgcagctg tcgcgcggct tccgcgcgct caaggtctgg
     7561 atgacgatca aggaacacgg cctcgaccgt ttcggccggc tgatcgatcg caacatcgag
     7621 caggcgcagg aactcgcggc gctgatcgcg gcggagccgc agctccagct cgtggcgccg
     7681 gtgccgacca acatcgtctg cttccggttc gatccgggtg gccgcgacac ggccgcgctg
     7741 aacgcgctga accaggaact gctgatccgc ctgcacgaaa gcggcatcgc cgcgccgtcg
     7801 tacacgacgc tcggcgaggt ctattgcctg cgggcggcga tcaccaactt ccggacgacc
     7861 agcgaggacc tgcgcgtgct ggtgcgcgcg gtcgtcgaga tcggacggtc tctcgtcaat
     7921 tgacggggag cggcagcatg ccgagcgcct cggcgtgcag gctctgcacg tagagctgtc
     7981 cctggtgctc cgtcgcaccg ctcgtttcgg gcagccgccc ctcgggatcc tgcaggtccg
     8041 ccacgacccg gccgctgtcg tcgaacgcga tcacgtggcc atacgcgggc ggcaccggcc
     8101 acagcgctcg cggcaggcgc aacgtcagcg aacgcagcca gggcttcgcc gccatgccgt
     8161 cgatcgtgtc gctgcgtggc ttcgtgagtc cgacccagta gcggccgtcg ccgccgcgcg
     8221 tcaggttgtc ggggaagcca ggcaggtcgt ccagcaggac ccggacgtcc ggctggcgcg
     8281 cctgcagtgc cgcgccgacg tcgagcaggt tcgcgttgcg cggcagctcg aggatgcggt
     8341 aggtgccggt ctcggcgacg agcagcgaac gctcgtccgc ggtcagcgcg ataccgttgg
     8401 gaaagcagag gccgttcgcg acgatgcgcg tcgcaggggc ggccggatcg tactcgagca
     8461 cgcgccccgt gcacgagtgc tcgaggatgt cgagcagcgc cgcatcgaac gtgcccagct
     8521 cgtgcggcga aaagcgcatc gatgcatcgg tgaacaggat cttgccgtcg ctcgcgacga
     8581 ccacggcgtc ggcatagcgg atcggctcga gcgccaccga atccgccagc accacgacgg
     8641 caccgcccgg ctgcacggcc agcagcccgc gcaccgcgtc ggcgatcacc aggcgcccgg
     8701 tcgcctcgaa cgcgagcccg agcggacggc ccttcgtgtc cgcgtaggtc tcgacgccag
     8761 tgccgtccgg ttgcatccgc aacaccgcgc ccgagcgcac gcccgtgtag agcagcccgt
     8821 ccggtccgaa gacgatgtgt tcgggcccca cgtgcggtgc aatcgagacc aggcgcagtt
     8881 tcgcgagccg gtcgttccgc gcgtgcgggc ccgcgtagcc ggcgaatttc tgcgatgacc
     8941 acgccacggg ttcgatcggc acgggccaca gcacgaggta cgcgaccagg gccgcgagca
     9001 cggccatcgc tgcgttgacg accctgcgca tgggcggatc ctaggcggcg agatgcagca
     9061 gtgtacgagc ttccgcacac gtcgcgcact cgcgccccgc ttcgcgcgcg agggcgacga
     9121 gcgcctcgat cagctcaccg ttgccgcgcg ccttgcggcc gtccggcagg tagaacgtgt
     9181 cctcgaggcc cgtgcgcagg ttgccgccga gttcagcggt gcgacggtgc agcggccaga
     9241 cgttctcccg gccgatcgcg gtgacctgcc acgtcgcggc ctgcggcaat agtccgatca
     9301 gcagcggcaa cagggcgggg tcggccggca tgcccgaatc gacgcccatc acgaagttga
     9361 cgtccgcatg accgcggtag agccccgtcc tcaggtacat ctcgaccgaa cggacgatgc
     9421 cgacgtcgaa gcactcgaac tccggcatcg caccggtttc ctgcatcgcc tgcaggaacg
     9481 cagcgacctt ctcgaccggg ttgtcgaaca gcagcggcgg ccaggcccag cgtccgtcgc
     9541 tgcgcacctt gaggtagttg agcgaaccgg cattggcggc cgcgatctcg ggccggatcg
     9601 cgcgcatgca ggccaccggc cccgagatgt cgggcccgat gacgccggtg gactggttga
     9661 acacgacgcc ggggcaggcc tcgcggatcg cctgcgcgat ctcgagggcg acctgcggat
     9721 cccacgacgg caggtgcccg cgcccgggtg cctgctgccg gaaatgcacg tgcacgacgc
     9781 tggccccggc gtcgcaggcg gcgcgggcct cgcgcgccat ctgctccggc gtgaccggca
     9841 cgtggtgctg cgccgggtcg gtcagcacac ccgtcagcgc gcaggtcacg acgatcttcg
     9901 aggacgaggc catcacggtt ctcctgcgta acgaacgagc acctgccccg gcaccacctg
     9961 gtcaccggct cgcacgagga cctcagcgac gaccgcgtcg cgcgtggcgc ggatctcgtg
    10021 ctccatcttc atcgcctcga gcacgaccag gcgatcgcct ttcgccacga gtgcccccgc
    10081 cgcagcggcc acgtccacga cgcgcccgtt catcgacgag cgcacttcgc gcaccgcgtc
    10141 gccggtggca ccggccgccc gcgccgacgc gcgccgcaga cactcgaagg cgaagtcgcc
    10201 gtgcgcgacg tgcagccaga gcgtcgcgcc atcccaggca tggcgatact cgaccggctc
    10261 gccgtcgatg gtcacgcgcg cgtggcgacg cggcgcggcc gccggtccct cgaccacgcc
    10321 ctggtcctgt cccgcaacga cgacgagcgc acgcggacct tcgagcacga cgcgaccctg
    10381 gcgctcgcac gggccggctg cggccgcgga ctgcgaggcg ttccagcgca gccgccaggg
    10441 ctgcggcagc ggccggccag tgccccagtg acgccagggc tcggccgtcg ccagcgactc
    10501 gggcgccgcg gtgaccgaca gccacgccgc gagcgaccac gtgcgcgcgt ccggcaccgg
    10561 ctgccggctc gc
//
LOCUS       DASPFR010000025         4114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1718245, whole genome shotgun sequence.
ACCESSION   DASPFR010000025 DASPFR010000000
VERSION     DASPFR010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718245"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VD737_00620"
     CDS             <1..651
                     /locus_tag="VD737_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HXW09091.1"
                     /translation="EAALRAAGANVLATDLVLTGVASRAFCNVRPPGHHAERAATMGF
                     CFFNNVAVAARHALDHHGLERVAVLDFDVHLGNGTEDILRDDERVLICSSYQHPLWPD
                     LDPPTVPGRIINCPLPPGSGSEAFRAVVSEHWLPALDAFRPQLLLVSAGFDAHAADDM
                     ARLRLGAADYGWVTDLVCAVAARHADGRVVSTLEGGYDLPALAASAATHIERLMLA"
     gene            complement(661..1401)
                     /locus_tag="VD737_00625"
     CDS             complement(661..1401)
                     /locus_tag="VD737_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09092.1"
                     /translation="MVTTAIPSRTRWTAERRFFTGLAIAAFIVVYVGFARTFFLRPWF
                     PDAPAPSESIFLVHGVVFACWSVLLVVQASLVALGRYAVHRTLGIVGVALAASMVVLG
                     VAGALVAAGRSTGFVGLPVPPLQFLIVPIADMTLFATFVALAVLRRYDAQAHKRWMIL
                     ATVNLLVAAFARWPGVISIGNPFVYMGLADLFIVALVAWDLRTRGRLHPATLWGGLAV
                     IASQPLRLALSGTAAWLAVAGWAVSLVR"
     gene            complement(1435..2637)
                     /locus_tag="VD737_00630"
     CDS             complement(1435..2637)
                     /locus_tag="VD737_00630"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09093.1"
                     /translation="MTRRPALKVVATCPEETKSALAAELQALGVDEMRPVFRAIEFEA
                     TPAQLYELHLRVRTASRLLRVIKDVPCHTPQMLHSQVRRLHWPEWFDRRHGFMVEPVG
                     LEAGGAMSPKQVVTEVREGVREAFTAAGQAPPPVDTQEPRVVIVAHLARGRCTLSFDT
                     SGKSLHKRGYREPGHPAPLKETLASAILQFAGYDGTQPFLDPMCGSGTLAIEAAMIAV
                     RKAPQIHRRKGEFHFEWLADFDRALWRETQERVRAGKLEAPPAPLVASDIEPRFVEMA
                     RKAALKARVERYLRFETARVQDVVPPAEHGVLVTNLPYGDRIGGGEADLRRLYGEVGD
                     VLKQRFAGWSAAVLAAEASPYKAIGLKPRRTIELRNGSLPCRLLLFDLYHGTRRAARE
                     EPVARGPC"
     gene            complement(2634..3467)
                     /locus_tag="VD737_00635"
     CDS             complement(2634..3467)
                     /locus_tag="VD737_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09094.1"
                     /translation="MAAVRTPGPLLTLPLHERLLGARRRAPASLEISVDLGRSTTVAG
                     IEAGSWSCDGGRYPYLERCRDRTVYWWTGTAFEPVARYGSALIKLVPTSWGPPTFEVD
                     GIKMLPTSKVSPYEDARRKVAAVQPRGRAVLDTCGGLGYFAACCVEQGAGRVLSFEKN
                     PDVLWLRTLNPWSPSPGGPLELVQGDATERVRSLPDRAFDAVLHDPPRFGIAGELYSA
                     DFYAHLSRVLRPGGRLFHYTGTPNKLTSGRDVAREVSRRLGSVGFRAEAWGDGVLALR
                     A"
     gene            complement(3555..3725)
                     /locus_tag="VD737_00640"
     CDS             complement(3555..3725)
                     /locus_tag="VD737_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09095.1"
                     /translation="MSTAGDSWDPYDVWLTRVKQPRDRLPQRATLVAEVRRDTTVTDT
                     KSSEPLRLRAVT"
     gene            complement(3930..>4114)
                     /locus_tag="VD737_00645"
     CDS             complement(3930..>4114)
                     /locus_tag="VD737_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09096.1"
                     /translation="SENQDERHAANDHEAPTQCRRQVGAPGRILLGGGAPGIRRSHAE
                     RESAVCPHGLLRCSVV"
BASE COUNT          631 a         1472 c         1427 g          584 t
ORIGIN      
        1 gaagcggccc tgcgggcggc cggggccaac gtcctcgcca ccgacctcgt gctcaccgga
       61 gtcgcatcac gcgcgttctg caacgtgcgc cctcccggcc atcacgcgga gcgcgctgcg
      121 acgatggggt tctgcttctt caacaacgtg gccgtcgcgg cccggcacgc gctcgaccat
      181 cacggactcg agcgggtcgc cgtgctcgac ttcgacgtcc acctgggcaa cggtaccgag
      241 gacatcctcc gcgacgacga acgcgtgctg atctgctcgt cgtaccagca cccgctgtgg
      301 cccgacctcg atccgccgac ggtgccgggc cgcatcatca attgccccct gccaccgggc
      361 agcggcagcg aggcattccg cgcggtcgtg tcggagcact ggctgccggc actcgatgcg
      421 ttccgcccgc aactgctgct cgtctccgcc ggtttcgacg cgcacgcggc ggatgacatg
      481 gcgcgcctgc gcctcggagc ggcggactac ggatgggtca ccgacctcgt atgcgcggtg
      541 gccgcgcggc acgccgatgg ccgcgtcgtc tcgacgctcg aaggcggtta cgacctgccg
      601 gcgctcgcgg cgagtgccgc gacgcacatc gaacgcctga tgctggcgtg atcgcgccgc
      661 tcagcgcacg agcgaaacgg cccagccggc gacggccagc caggccgccg tgcccgacag
      721 ggcgaggcga agcggctgcg acgcaatcac ggcgagcccg ccccagagcg tcgcggggtg
      781 cagacgaccg cgcgtgcgca ggtcccacgc gacgagcgcg acgatgaaca ggtccgcaag
      841 tcccatgtag acgaacgggt tgccgatcga gatgacgcct ggccagcgcg cgaacgccgc
      901 gaccagcagg ttgaccgtgg ccaggatcat ccagcgcttg tgcgcctgcg cgtcgtatcg
      961 gcgcagcacc gcgagcgcca cgaacgtcgc gaacagcgtc atgtcggcga tcggcacgat
     1021 caggaactgc agcggcggaa ccggtaagcc gacgaaaccc gtcgatcggc ccgccgcaac
     1081 cagggcacca gccacgccca ggacgaccat cgacgcggcc agtgccacgc cgacgatgcc
     1141 gagcgtacga tgcaccgcgt agcggccgag cgcgacgagc gaggcctgca ctacgagcag
     1201 cacggaccag cacgcgaaaa cgacgccgtg cacgagaaag atcgattcgc tcggggcggg
     1261 cgcgtcgggg aaccagggcc gcaggaagaa cgttcgcgcg aagccgacgt agacgacgat
     1321 gaacgcggcg atggcgaggc ccgtgaagaa ccggcgctcg gcggtccagc gtgtgcgcga
     1381 aggaatcgcc gtagtgacca tgacgcggtc cccttcttgt tctctgcggc gagcctagca
     1441 cgggcctcgc gcgacgggct cctcgcgcgc ggcgcgccgt gtgccgtggt agaggtcgaa
     1501 cagcaggagc cggcacggga ggctgccgtt cctgagttcg atcgtgcgac gcggcttgag
     1561 cccgatcgcc ttgtacggag acgcctcggc cgcgagcacc gcggcgctcc agcccgcaaa
     1621 ccgctgcttg agcacgtcgc ccacttcgcc gtacaggcga cgcaggtcgg cctcgccgcc
     1681 gccaatgcgg tcgccgtacg gcaggttcgt caccagcacg ccgtgctccg cgggcggcac
     1741 cacgtcctgc actcgtgccg tctcgaagcg caggtatcgc tcgacgcgag ccttgagcgc
     1801 cgccttgcgc gccatctcca cgaagcgcgg ctcgatgtcg gacgcgacga gcggcgccgg
     1861 cggggcctcg agcttgccgg cacggacgcg ctcctgcgtc tcgcgccaga gcgcgcggtc
     1921 gaagtccgcg agccactcga agtggaactc gcccttgcgg cggtggatct gcggcgcctt
     1981 ccgcaccgcg atcatcgcgg cctcgatcgc cagcgtgccg ctgccgcaca tggggtcgag
     2041 gaacggctgc gtgccgtcat acccggcgaa ctgcaggatt gccgaggcga gcgtttcctt
     2101 gagcggcgcc gggtggcccg gctcgcgata gccgcgtttg tgtagcgact tgcccgacgt
     2161 gtcgaaactg agcgtgcacc ggccgcgcgc caggtgggcg acgatcacga cgcgtggctc
     2221 ctgcgtgtcg accggcggtg gggcctggcc cgctgccgtg aacgcctcgc gtacgccctc
     2281 gcggacttcg gtgaccacct gcttcgggct catcgcgccg cccgcctcga gacccaccgg
     2341 ttcgaccatg aagccgtgcc ggcggtcgaa ccactcgggc cagtgcaggc gccgcacctg
     2401 cgagtgcagc atctgcggcg tgtggcacgg cacgtccttg atcacgcgca gcaggcggct
     2461 cgccgtgcgc acgcgcaggt gcagctcgta taactgcgcc ggcgtggcct cgaactcgat
     2521 cgcgcgaaac accggccgca tctcgtcgac gccgagcgcc tgcagttcgg cggcgagtgc
     2581 ggacttggtt tcctccgggc aggtcgcgac gactttcagc gcgggtctcc gtgtcatgcc
     2641 ctgagcgcga gcacgccgtc gccccaggcc tcggctcgaa aaccgacgct cccgagccgc
     2701 cgtgacactt cgcgtgccac gtctcgacca ctcgtcagct tgttcggcgt gcccgtgtag
     2761 tggaacagcc ggccgccggg gcgcagcacg cgcgacaggt gcgcatagaa gtccgcggaa
     2821 tagagctcgc cggcgatgcc gaacctcggc ggatcgtgca gcacggcgtc gaacgcacgg
     2881 tcgggcagcg accggacgcg ctcggtcgcg tcgccctgca cgagctcgag cgggccgccc
     2941 ggcgacggcg accaggggtt gagcgtgcgc agccacagca cgtccgggtt cttctcgaac
     3001 gagagcacgc gcccggctcc ctgctcgacg cagcacgccg cgaaatagcc cagcccgccg
     3061 caggtgtcga gcacggcgcg accgcgaggt tgcacggcgg cgaccttgcg acgcgcgtct
     3121 tcgtacggcg acaccttcga ggtcggcagc atcttgatgc catccacctc gaacgtgggt
     3181 ggtccccacg acgtcggcac gagcttgatc agagccgagc catagcgtgc caccggctcg
     3241 aacgccgtgc cggtccacca gtagaccgtg cgatcgcgac agcgctccag gtacggatag
     3301 cggcccccgt cgcacgacca ggatccggct tcgatgcctg ccacggtcgt ggagcggccg
     3361 aggtcgaccg agatctcgag cgacgccggc gcgcgtcgtc gggcgcccag caggcgctcg
     3421 tgcagcggca acgtgaggag cgggcccggt gttcgaactg ccgccatgac ccgttcgcga
     3481 cgccccacga acggggcgcc gcgattatcc actgtgccgg cccaggcgtg gcggcgggcg
     3541 ggcaggaggt gccgtcaggt gacggcgcgc aggcgcaggg gctcgctcga cttggtatcg
     3601 gtcacggtgg tgtcgcggcg gacctcggcc accagcgtgg cgcgctgcgg caggcggtcg
     3661 cgcggctgct tcacgcgcgt gagccagacg tcgtacggat cccacgagtc gccggcggtc
     3721 gacactgcgt cagtcccacg cgctgcggac acttcctgtg gcaacaccat ctcgataccc
     3781 tccctagaca ggtcgagttc ttgttgttct tggccattga aacagggcat ccagccccgc
     3841 tcctgagtga acccggcgcc cacgactttg ctcgtgtgac accgggacgg cgaagtctcc
     3901 gcgcggtcgc ggaccttgtc aactgactcc tacacgacgc tgcaacgcag caggccgtgc
     3961 gggcaaacgg cggactcgcg ttcggcgtgg cttctgcgaa tgcccggcgc gccgccgccc
     4021 agtaggatgc ggcccggtgc gcccacctgg cggcggcact gcgtgggggc ttcatgatcg
     4081 ttcgctgcat gtcgctcgtc ctggttttcg ctgc
//
LOCUS       DASPFR010000026         3037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1596574, whole genome shotgun sequence.
ACCESSION   DASPFR010000026 DASPFR010000000
VERSION     DASPFR010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596574"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(474..>3037)
                     /locus_tag="VD737_00650"
     CDS             complement(474..>3037)
                     /locus_tag="VD737_00650"
                     /inference="COORDINATES: protein motif:HMM:NF009415.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HXW09097.1"
                     /translation="LLRDDAMSAVLLRFLWFVPLKYKRRFIAAIDMHLCDRYPMFRGL
                     SQGWPGETCIPPYIRPPGERANDFELVNQGYLGYMKLGYSRREVELFVWLEVLRDKQC
                     QDRPCEIGVLLANRKEPKGGCPVKIHIPEMLDLLGQGKFRQALELIESCNPLPNVTGR
                     VCPQELQCQGVCTHTRRPIEIGQLEWFLPQHDKLVNPDVAGRFARTVSPWARAEKPPI
                     AVVGSGPSGLINAYLLAVEGFPVTIFEAFHELGGVLRYGIPEFRLPNELIDDVVQKIT
                     LLGGRFVRNFVVGKTATLDELKAAGFWKIFVGTGAGLPTFMNVPGEHLLGVMSANEFL
                     TRVNLMRAIDQKYETPLPETHGKEVFVIGGGNTAMDAARTARRLGANVTIVYRRTIRE
                     MPARVEELHHALEEGIRVRELRAPTEFLGDGKTHWVTHAVLDVNELGPADPSGRRSPV
                     RTGQVERVPVDLVIMALGNTANPIMTEAEPGLRTTKWGTIALDAQGSQQTSIADVYSG
                     GDAARGGSTAIRAAGDGQAAAREIVGDVPFTAEEIRMRVETAARYTELGQASPTIVSK
                     TDLAGGIVEFRIRAPLVARSARAGQFVRVLGWGDGELVPLTLADWDVAAGTIDLVIQG
                     VGTSSMLMNRMAVGDSFAGIAGPLGRPSEIHEYAVDETVVFTAGGVGLPPVYPILRQH
                     LRRGNHCTLIAGFRSTDLLFWTGEQERVGRLQAEFGDQLDVIYTSNDGTFGQKGFVTT
                     PLEAMLQDGALSKGRRIVEVVTIGPPLMMRAVSDLTRPYGVKTVASLNSIMVDATGMC
                     GACMVPVTIDGRLVRKHACIDGPELDAHIIDWDKFLPRFNAFRSQEARSRAAHGL"
BASE COUNT          487 a         1038 c         1006 g          506 t
ORIGIN      
        1 ttacagactt cggggacgtc cagccccgcc tcgcacgagc cgaggctcgc gagcacgtcc
       61 gcgggcggca gtcgcgtaat ttccatagcg ccccgatgac ggggctcggg acattttccc
      121 gcttaccggg gtgagcctgc agcacgtgca gcgaggcggg ctctcggact atgcctaggc
      181 ggggtacggc cgaccgcctc acttgtcctc gttccagatc tgccagagga ctttccacga
      241 accgttgggc tgccgcacgg cagcattcgg acacggggac agatatgggg acactccgaa
      301 ttaacggtta attggggaca ctccttattt tcgatgaaac ccggcgatcg caggggacat
      361 ctgcgcgacc gcccgcggcg gcggcgtcga aaaccgctgc atccgcagca cgaaaaacag
      421 gagtgtcccc aattaagtgt ccccaattaa gggaaaattc ggagtgtccc catttagagg
      481 ccgtgggcag cgcggctgcg ggcttcctgc gacctgaacg cgttgaagcg cggcaggaac
      541 ttgtcccagt cgatgatgtg cgcgtcgagc tcgggcccgt cgatgcaggc gtgcttgcga
      601 acgagcctgc cgtcgatcgt gacgggcacc atgcaggcgc cgcacatccc ggtcgcgtcc
      661 accatgatcg aattcaggct cgcgacggtc ttcacgccgt atggtcgcgt caggtcgctg
      721 accgcacgca tcatcagcgg cggaccgatc gtgacgacct cgacgatccg gcggcccttg
      781 ctgagcgcgc cgtcctgcag catcgcctcg agcggcgtcg tcacgaagcc cttctggccg
      841 aacgtgccgt cgttcgaggt gtagatcacg tccagctggt cgccgaactc ggcctgcagg
      901 cggccgacgc gctcctgctc gccggtccag aacagcaggt ccgtcgagcg aaagcccgcg
      961 atcaacgtgc agtgattgcc gcggcgcagg tgctggcgca ggatcggata gaccggcggc
     1021 aggccaacgc cgcccgccgt gaacacgacc gtttcgtcca cggcgtattc gtggatttcg
     1081 ctcggcctgc cgagtgggcc cgcgataccg gcgaacgaat cgccgaccgc catccggttc
     1141 atcaacatcg agctcgtgcc gacaccctgg atcacgaggt cgatcgttcc cgcggcgacg
     1201 tcccagtccg cgagcgtcag cggcaccagc tcgccgtcgc cccagccgag cacgcgcacg
     1261 aactggccgg cgcgggccga acgcgcgacg agcggtgcgc ggatgcggaa ttcgacgatg
     1321 ccgcccgcca ggtcggtctt cgacacgatc gtcgggcttg cctggccgag ctcggtgtag
     1381 cgcgctgccg tctcgacacg catacggatc tcttccgccg tgaacggtac gtcgccgacg
     1441 atctcgcgcg ccgcggcctg tccgtcgccc gccgcccgga tcgcggtcga cccgccgcgc
     1501 gcggcgtccc cgcccgaata cacgtcggcg atcgaggtct gctgcgatcc ctgcgcatcg
     1561 agcgcgatcg tgccccactt ggtcgtgcgc agccccggtt cagcctcggt catgatcggg
     1621 ttggcggtgt tgccgagcgc catgatcacg agatcgaccg gcacgcgctc gacctgtccg
     1681 gtccggaccg ggctgcggcg gcccgacgga tcggcgggcc cgagctcgtt gacgtcgagc
     1741 acggcgtgcg tgacccagtg cgtcttgccg tcgcccagga actcggtcgg cgcgcgcagc
     1801 tcgcggaccc tgatgccttc ctcgagcgcg tggtgcagtt cctcgacgcg cgccggcatt
     1861 tcgcggatcg tgcggcgata gacgatcgtg acgttcgcgc cgaggcggcg cgccgtgcgg
     1921 gcggcgtcca tcgcggtgtt gccaccgccg atcacgaaga cttccttgcc gtgcgtctcg
     1981 ggcaacggcg tctcgtactt ctggtcgatt gcgcgcatca ggttgacgcg cgtcaggaac
     2041 tcgttcgccg acatgacgcc gagcaggtgc tcgcccggca cgttcatgaa cgtcggcagc
     2101 ccggcgcccg taccgacgaa gatcttccag aagcccgcgg ccttgagctc gtcgagcgtc
     2161 gccgtcttgc cgacgacgaa gttgcgcacg aagcggccgc cgagcagcgt gatcttctgc
     2221 acgacgtcgt cgatcagctc gttcggcagc cggaactcgg ggatgccgta gcgcagcacg
     2281 ccgccgagtt cgtggaacgc ctcgaagatc gtgacgggaa agccctcgac cgccagcagg
     2341 taggcgttga tcagcccgga aggccccgag cccacgaccg cgatcggcgg cttctcggca
     2401 cgcgcccacg ggctgaccgt cctcgcgaac cggccggcga cgtccggatt cacgagcttg
     2461 tcgtgctgcg gcaggaacca ctcgagctgg ccgatctcga tcgggcggcg cgtgtgcgtg
     2521 cagacgccct ggcactgcag ttcctgcggg cacacgcgtc ccgtgacgtt gggcagcggg
     2581 ttgcagctct cgatcagctc gagcgcctgg cggaacttgc cttggccaag caggtcgagc
     2641 atttccggaa tgtggatctt gaccgggcag ccgcccttcg gctccttcct gttcgcgagc
     2701 agcacgccga tctcgcaggg ccggtcctgg cactgcttgt cgcgcaacac ctcgagccag
     2761 acgaacagct ccacttcgcg acggctgtag ccgagcttca tgtagccaag gtagccctgg
     2821 ttcacgagct cgaagtcgtt ggcgcgttca ccgggcggcc ggatgtacgg cggaatgcag
     2881 gtctcgccgg gccagccctg cgacaacccg cggaacatcg ggtagcgatc gcagaggtgc
     2941 atgtcgatgg cggcgatgaa ccgccgcttg tacttgagtg gcacgaacca caggaagcgc
     3001 agcagcacgg cgctcatcgc gtcgtctctc agcagca
//
LOCUS       DASPFR010000027         8572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_253648, whole genome shotgun sequence.
ACCESSION   DASPFR010000027 DASPFR010000000
VERSION     DASPFR010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253648"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VD737_00655"
     CDS             complement(<1..587)
                     /locus_tag="VD737_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09098.1"
                     /translation="MSSRRALLRHLWSMIQTRAEAAGLVVQLQRTAVTQGLLFGGIAA
                     VTGLSFMTAVIVWIAVAAPPSWRGWALGIVSIALLAAAILAAVTASRKISRDAGVIAD
                     YSRGLKLDLAMISLALKDADTDDEEKLAERERAKEKVREAAADKAAAPSTAEGGDTPG
                     PGDPAMESATAAMRAAKPRPDVSPAGPDAEREAAG"
     gene            complement(598..1020)
                     /locus_tag="VD737_00660"
     CDS             complement(598..1020)
                     /locus_tag="VD737_00660"
                     /inference="COORDINATES: protein motif:HMM:NF017742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HXW09099.1"
                     /translation="MYGNNAGLATDPNTSSTTMSGDNNFSTGNVSARHQETMTQLNRT
                     VAQAEELLKALGNESGEAIDAMRARVSATLRDARERLSGGAGQARTFANNSLSQADAF
                     VHANPWRAVAIGAGIGALLAILMSSGRGGHDYDRVDID"
     gene            complement(1257..1442)
                     /locus_tag="VD737_00665"
     CDS             complement(1257..1442)
                     /locus_tag="VD737_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09100.1"
                     /translation="MRIGRTIGLLTLFAGLTTWFATRAALQRQHSKQRNETEDMTRWE
                     SDGGATTAGPQPPGTQL"
     gene            complement(1550..4282)
                     /gene="ligD"
                     /locus_tag="VD737_00670"
     CDS             complement(1550..4282)
                     /gene="ligD"
                     /locus_tag="VD737_00670"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HXW09101.1"
                     /translation="MPTSVPRALERYRAKRHFDRTPEPAGARRRSSQRLEFVIQRHHA
                     RRLHYDFRLEWDGVLKSWAVPKGPSLDPAEKRLAVQVEDHPFEYRTFAGDIPKGEYGA
                     GHVIIWDRGHWTPEGDVDAGMHDGKLDFALEGERLRGRWTLVRLKDAHGEKANWLLIK
                     RRDEHADAGGPEVTELYTDPVPGTAPQSGASSTREGGASEKPARSSRRGTRAAARSTV
                     ASTRAEQARARSSRRRTAPVFIPPQLATSVDTAPSSGRWLTELKFDGYRIVAWVDAGR
                     VHCYTRSGHDWTHRIPHVSAALADLGLRDAWLDGELVAVDERGIPQFQRLQQALDPHG
                     GERPLFMVFDALQLSGADLRAKPQRERKRLLQQALADLPPTGPLRLVDFVDGDSVALR
                     EQACAQGYEGVILKDAEADYRSGRNRAWLKLKCRREQEFVIGGYTRTAAGRDTLTALL
                     LGYHDERGRLRFAGRAGSGFSGKQLAALRQRLDRLAAETAPFADPPKLRGKEQPQWVR
                     PELVAQVRFAEWTESGILRQPLFLGLREDIGPAGVRREPDRILDSSGGAAAESRGKSK
                     VAIKKTNARSARSQTPRAARKTRAAGKAPVARATRTRRASAPAATAPSRRRTTVQLTN
                     PQRVLYPTDGVTKQQLAEYYEAIAPVLWPHLKARPLSLIRATSAQGRVFFQRHIDGDA
                     FPGLKPVTIPGSDEEPYFVCRSLDSIPLLAQVGAVELHTWGSHVPHAERADRLTFDLD
                     PDPALPWPRVREAATLVRQLLEELGLRSRLKTSGGLGLHVVVPLAGGPPMEVAATFSR
                     RVAEHLARLIPQRFSARRGAANRQGKVYVDWQRNQFAATTVSAYSPRHRAGVPVSLPV
                     SWDELGSKDIRAAWFNLRNVAARIADRGDAWADEPPLRQTLTQRVIDRLEAAAA"
     gene            complement(4292..5209)
                     /locus_tag="VD737_00675"
     CDS             complement(4292..5209)
                     /locus_tag="VD737_00675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HXW09102.1"
                     /translation="MARVIWKGAISFSLIHIPVSLYPASRSASLDLTMLDKRDFSPIG
                     YQRVNKATGKTVEWGDIVKGYEYEKGDYVVLSDEDFRQANVEATQTIDIQGFVELSQI
                     PPMYYDTPYYLAADKRGEKVYTLLREALVKSNTVAIGLVVIRTRQHVCALLPVGETLL
                     LNTLRYADEVLDPAEHAPATKSLEEARVSQREFGMAVKLVEDMRLDWAPAEFHDSYRE
                     DLLRRIEEKVEAGQTKTLTPASKAAPVRKAEVVDLTELLKRSLEQRGGGGRRSSATPR
                     ANAASEPEATPSRARKAASGNGPRRRRSA"
     gene            complement(5257..6114)
                     /locus_tag="VD737_00680"
     CDS             complement(5257..6114)
                     /locus_tag="VD737_00680"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HXW09103.1"
                     /translation="MNTRYVPIAAVTALLTATPIVAQPQTTTDQDTSGTTATETTPST
                     SSSAEASTSGTSSTSSSSASGTPDSSGAHATGPTSSSTPGSSTSTGYDTAGGNTMSGA
                     TGTAGTSDSDDTAEADAATSAATATGSAAGAAAGTMTAGSNQLQGQDATFLRKALEGD
                     RMEIASAQAALDQAQRTDTKNAARMILEDHRNSSQKLQSLASRKGWTLPSAASADADE
                     AMTSTSGSGSFDDRFLADQIRMHREAIALYRAQASAGSDPDLREFARDQLPHLEHHLE
                     MLQGSNTQK"
     gene            6267..7544
                     /gene="egtB"
                     /locus_tag="VD737_00685"
     CDS             6267..7544
                     /gene="egtB"
                     /locus_tag="VD737_00685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HXW09104.1"
                     /translation="MTSSSGSTAAQATHLADDYRRIRQTTRILAEPLSAEDAALQSMP
                     CTSPVKWHLAHTSWFFEQNVLRVARGGSHVAFDRSFDRLFGPAWPRERRGLVSRPDLE
                     TVLDYRDWIDLRVLALLADPAEGSCHVEVIERGLQHEQRHQERLLADVKHLLAFHPAA
                     PAYYPRPAAVPGPDAAAGTPLRMVEIEGGLQWLGAGEAGFAFAQERPRHRHWLEPCAL
                     GERLVTNGEYRAFVDAGGYERAEFWSVEGWRARVAQGWRAPLYWSLEEPAMHFTLGGL
                     QPLDALAPVVHVSRHEAEAYARFAGARLPTEAEWECAARRVPLHGPFLESGTFAPFAA
                     PTTPSPLLQQVYGVAWQWTRSAATPYPGAHDDGPHASRASGVRYDSTQGVARGGSCIT
                     PSGHVRPSSRLFLNPDARWQFTGIRLAKDCPAS"
     gene            7541..8521
                     /locus_tag="VD737_00690"
     CDS             7541..8521
                     /locus_tag="VD737_00690"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HXW09105.1"
                     /translation="MRNAIDVARHTRVAPPHAARPLIALVFEGLSEEQKQLPSMLAFD
                     PVGSQLFERVCEQPEYFLARAEATLLRRHAAEIGAVVGPQAAIVEYGAGAGRLGALLL
                     DAMTSAHSYVPIDVDATQLARCREAIRASGRALRIHPLHQDFRQFVALPTTLGGARRR
                     VAYFPGSSVGSFRQLEAVALLNSMRETVGPGGGLLVGIDLLKEPQVHLRSWLDAAGTV
                     AAFNGNVLLRLNREIDATFDPAGFEHRVAWNESERRLEMGLVSLRAQAPTIAGIGVAL
                     AAGEEIRTSVAHKYSLEEFASLAHIAGWVARSTWVDTEHDYALQYLEAGE"
BASE COUNT         1118 a         3021 c         3027 g         1406 t
ORIGIN      
        1 ctgcccgcgg cctcgcgctc ggcgtccggt ccggcgggcg agacgtctgg gcgcggcttt
       61 gccgcgcgca tcgccgcggt cgcggattcc attgcggggt cgcccggccc cggcgtgtcg
      121 ccgccttcgg cggtgctcgg cgccgcggct ttgtccgccg ccgcctcgcg caccttctcc
      181 ttggcacgct cgcgttcggc gagcttctcc tcgtcgtcgg tgtcggcgtc cttgagcgcg
      241 aggctgatca tcgcgaggtc gagcttcagg ccgcgcgagt agtcggcgat cacgcccgcg
      301 tcacgggaaa tcttccggct cgccgtgacc gcggccagga tcgccgccgc gagcaaggcg
      361 atcgagacga taccgagcgc ccagccccgc caggacggcg gggcggcgac ggcgatccag
      421 acgatcacgg cggtcatgaa cgatagtccg gtgaccgccg cgatcccgcc gaagagcagg
      481 ccctgcgtca ccgcggtgcg ctgcagctgc acgacgaggc cggcggcctc ggcgcgcgtc
      541 tggatcatgg accagagatg acgcagcagt gcacggcgcg atgacacggg ccacccgtca
      601 gtcgatgtcg acgcggtcgt agtcgtggcc gccccggccc gacgacatca ggatcgcgag
      661 cagcgcaccg atgccggcgc cgatggcgac cgcccgccac ggattggcgt gcacgaacgc
      721 atccgcctgc gacaggctgt tgttggcgaa cgtccgggcc tggccggctc cgcctgaaag
      781 ccgttcacgc gcatcccgca aggtcgcgga tacgcgcgca cgcatcgcgt cgatcgcctc
      841 gccgctctcg ttcccgagcg ccttgagcag ttcctcggcc tgggcgacgg tgcggttcag
      901 ctgcgtcatg gtctcctggt gacgggccga cacgttgccc gtcgagaaat tgttgtcccc
      961 cgacatcgtc gtcgacgacg tgttcggatc ggtggccagt ccagcgttgt ttccgtacat
     1021 atgtagctcc ttccatggtc gattcggcgt gacggctgca ggcccgtccg gttggcggcg
     1081 tcggcgacgc gtgcggctcg atagcgattt cccgcagcgt cgcgaccggt cggcacgcga
     1141 atcctgcgga tgtccgattc tccgtcccag ggggggcggg gctatcggtt caacccccac
     1201 ccctgcctca gggcgacccg gagccgcgtc ggtgcatgcc taccggcgcg gtgcggtcag
     1261 agctgcgttc cgggcggctg cggtccggcg gtcgtggccc cgccgtcgct ctcccagcgg
     1321 gtcatgtcct ccgtctcgtt gcgctgcttc gagtgctgtc gctgcagcgc ggcccgtgtc
     1381 gcgaaccagg tcgtcagccc ggcgaacagt gtcaacagtc cgatcgtgcg tcctatcctc
     1441 atgatggctt tcctcggtgg tggtgtggtc gtgcgtatac ggcgacgcgc gcggcgcgcc
     1501 gccgggtcgg aacggggggt ggccgagtgg cggcctgcgt cgctcgtggt catgcggcgg
     1561 ccgcttcgag ccgatcgatc acgcgttgcg tgagcgtctg ccgcaatggc ggctcgtcgg
     1621 cccacgcgtc gccgcggtcc gcgatgcggg ccgcgacgtt gcgcaggttg aaccaggccg
     1681 cgcggatgtc cttgctgccg agctcgtccc aggagaccgg gagggacacc ggcacaccgg
     1741 cgcggtggcg cggcgagtag gcagacacgg tggtggccgc gaactggttt cgctgccagt
     1801 cgacgtacac cttgccttgc cgattggccg ccccgcgccg ggccgagaat cgctgcggga
     1861 tcaggcgtgc gaggtgctcg gccacgcggc gcgagaacgt ggcggccact tccatcggcg
     1921 gaccgcccgc gagcggcacg acgacatgca ggccgaggcc gccgctggtc ttgagccgcg
     1981 agcgcaaccc gagctcctcg agcagttggc gcaccagtgt cgcggcctct cgcacgcgcg
     2041 gccacggcag tgccggatcg ggatcgaggt cgaaggtcag gcgatcggcc ctctccgcgt
     2101 gcggcacgtg cgagccccag gtgtgcagtt cgacggcgcc gacctgggca agcaggggga
     2161 tcgaatcgag cgagcggcag acgaagtaag gctcctcgtc ggaaccgggg atcgtcacgg
     2221 gcttgagtcc gggaaacgcg tcgccgtcga tgtgtcgctg gaagaacacg cggccctgcg
     2281 cgctcgtggc gcggatcagt gacaacggtc gcgccttcag gtgcggccac aggaccggcg
     2341 cgatcgcctc gtagtactca gcgagttgct gcttggtcac gccgtccgtc gggtagagca
     2401 cgcgctgcgg attcgtcagt tgcacggtcg tgcgtcgacg cgacggcgcc gtggcggccg
     2461 gtgccgaggc gcgccgcgtg cgcgtcgctc gcgcgaccgg cgccttgccc gcggctctgg
     2521 tcttcctggc agcacgcggc gtctgcgacc gcgcgctgcg agcgttcgtc ttcttgatgg
     2581 ccaccttgga cttcccacgt gactcggcgg ccgcgccgcc gctgctgtcg aggatccggt
     2641 cgggctcgcg ccgcacgccg gcggggccga tgtcctcgcg caagccgagg aacagcggct
     2701 ggcgcaggat gccggactcg gtccactcgg cgaaccgcac ctgcgcgacg agttccggcc
     2761 gcacccactg cggttgctcc ttgccgcgca atttcggcgg gtcggcaaac ggcgccgtct
     2821 cggccgcgag ccggtcgagc cgctggcgca aggcggcgag ctgcttcccg gagaatcccg
     2881 agcccgcgcg accggcgaac ctcagccgcc cgcgttcgtc gtggtagccg agcagcagtg
     2941 ccgtgagcgt atcgcgcccc gccgccgtgc gcgtgtagcc gccgatcacg aactcctgct
     3001 cgcgacggca cttgagctta agccatgccc gattgcggcc ggagcggtag tccgcttccg
     3061 catccttgag gatcacgcct tcatagccct gcgcgcaggc ctgctcgcgc agagccacgc
     3121 tgtcgccgtc tacgaagtcg accagtcgaa gcgggcccgt cgggggcagg tccgcgagcg
     3181 cctgctgcag caggcgcttg cgctcccgct gcggcttcgc gcgcaggtcc gcgccggaca
     3241 gctgcagcgc gtcgaacacc atgaagagcg gacgttcgcc cccgtgcggg tcgagcgcct
     3301 gctggagcct ctggaactgc gggatgccgc gttcgtccac ggcgaccagc tcgccatcga
     3361 gccatgcgtc gcgcaggccg aggtccgcga gcgccgcgga cacgtgcggg atcctgtgtg
     3421 tccagtcgtg gccgctgcgc gtgtagcagt gcacacgacc tgcgtcgacc cacgcgacga
     3481 tgcgatagcc gtcgaacttg agctccgtga gccaacgtcc gctcgacggt gccgtgtcga
     3541 cggaggtcgc gagctgcggc gggatgaaaa cgggcgctgt tcgacgacgg ctcgagcgcg
     3601 cccgcgcctg ctccgcccgc gtcgacgcga ccgtggaccg tgccgccgcg cgcgtcccgc
     3661 gccgcgacga tcgcgcgggc ttctccgatg cgccgccctc gcgcgtcgag gaggcgccgg
     3721 actgcggcgc ggtgcccggc accgggtcgg tgtagagctc ggtgacctcc gggccgccag
     3781 cgtccgcgtg ctcgtcgcga cgcttgatca gcagccagtt ggccttctcg ccgtgcgcat
     3841 ccttgagccg cacgagcgtc cagcgcccgc gcaggcgctc gccctcgagc gcgaaatcca
     3901 gcttcccgtc gtgcatgccc gcgtcgacgt cgccctccgg ggtccagtgc ccgcggtccc
     3961 agatgatcac gtggccggcg ccgtactcgc ctttggggat gtcgccggca aaggtccggt
     4021 actcgaaggg gtggtcctcc acctgcactg cgagtcgctt ctcggccggg tccagcgacg
     4081 gccccttcgg cacggcccag ctcttgagca cgccgtccca ctcgagcctg aagtcgtagt
     4141 gcaggcggcg cgcgtggtga cgctggatca cgaactcgag ccgctgcgac gaccggcgcc
     4201 gcgcgccggc cggctcgggc gtccggtcga agtgtcgctt ggctcggtac cgctcgagcg
     4261 cgcgcgggac ggaagtcggc atgcgtgtcg gtcaggcgct gcggcgccga cgggggccgt
     4321 tgcccgacgc ggccttgcgg gctcgcgagg gcgtcgcttc aggttcggat gcggcgttcg
     4381 ctcggggcgt cgcggacgag cgccggccgc ctccgccgcg ctgctccagg ctgcgcttca
     4441 gcagttccgt caggtcgacg acctcggcct tgcggaccgg agccgccttc gaggccggcg
     4501 tcagcgtctt ggtctggccc gcctcgacct tctcctcgat ccgccgcagc aggtcctcac
     4561 ggtaggagtc gtgaaactcc gcgggagccc aatcgagccg catgtcctcg accagcttga
     4621 ccgccatgcc gaactcgcgc tgcgacacgc gcgcttcctc gagcgacttc gtggccgggg
     4681 cgtgctcggc ggggtccagc acctcgtcgg catagcgcag cgtgttgagc agcagcgttt
     4741 cgccgaccgg cagcagcgcg cacacgtgct gcctggtgcg gatcacgacc aggccgatcg
     4801 ccaccgtgtt cgacttgacc agtgcctcgc gcaacagcgt gtagaccttc tcgccacgct
     4861 tgtccgcggc gaggtagtac ggcgtgtcgt agtacatcgg cgggatctgc gacagctcga
     4921 cgaaaccctg gatgtcgatc gtctgcgtcg cctccacgtt cgcctggcgg aagtcctcgt
     4981 cgctcagcac gacgtaatcg cccttctcgt actcataccc cttgacgatg tcgccccact
     5041 cgacggtctt gccggtcgcc ttgttgacgc gctggtaacc aatcggcgag aagtcgcgct
     5101 tgtcgagcat cgtgaggtcc agcgatgccg aacggctggc cgggtacagc gacaccggga
     5161 tgtggatcag gctgaagctg atcgcgcctt tccagatcac ccgtgccatg tgcccacccg
     5221 ccggttcggc atcgcgccca tacgtcgtcg cccggcctac ttctgcgtgt tcgagccctg
     5281 cagcatttcg aggtgatgct cgaggtgcgg cagctggtcg cgtgcgaatt cgcgcaggtc
     5341 agggtccgag ccggccgagg cctgcgcgcg gtacagcgcg atcgcctcgc ggtgcatgcg
     5401 aatctggtct gccaggaagc ggtcgtcgaa gctgccgctg cccgacgtgc tggtcatcgc
     5461 ctcgtcggcg tccgcgctcg cggcgctcgg cagcgtccac cccttgcggc tcgccagcga
     5521 ctgcagcttc tggctcgagt tgcggtgatc ctcgaggatc atgcgcgccg cgttcttcgt
     5581 gtcggtgcgc tgggcctggt cgagcgccgc ctgggcggaa gcgatttcca tgcggtcgcc
     5641 ctcgagggcc ttccgcagga acgtcgcgtc ctgaccctgc agctggttgg agcctgccgt
     5701 catcgtgccg gcggccgcac cggcggcgga gccggtcgcc gtggcggcgg acgtcgccgc
     5761 gtcggcctcg gccgtgtcat cgctgtcgct cgtgccggcc gtgccggttg cgcctgacat
     5821 cgtgttgccg ccagcggtgt cgtaacccgt gctggtgctg gagcccgggg tgctcgaact
     5881 cgtcggaccc gtggcgtgag cgccgctcga gtccggggtg cccgaagcgc tggagctgga
     5941 agtgctcgag gtgccgctcg tcgatgcctc cgccgacgag ctcgtcgacg gtgtcgtctc
     6001 ggtcgccgtc gtgccgctcg tgtcctggtc ggtggtcgtc tgcggttgcg cgacgatcgg
     6061 cgtcgcggtc agcagcgccg tcaccgccgc gatcggtacg tatcgagtgt tcatggtggt
     6121 gctctccttg tcgggttgct cagcgtcctt gcttgcgtcg ccgttattgc aagcaccatc
     6181 acgacttgca ggcggctcag tggtgcttcc actgtaggct ccgacggaca gacagtctgc
     6241 gccgaaagcg cgttaatcac cgcatcatga cgagttcgtc cggatcgacc gcggcacaag
     6301 cgacgcacct cgccgacgac taccgcagaa tcagacaaac gacgcgaatc cttgccgagc
     6361 cgctgagtgc cgaggacgct gcgctgcagt ccatgccgtg cacgagtccc gtgaagtggc
     6421 acctggcgca cacgtcgtgg ttcttcgagc agaacgtgct gcgggtcgcc cgcggcggct
     6481 cgcacgtggc gttcgaccgc tcgttcgatc gcctgttcgg tccggcgtgg ccgcgcgaac
     6541 gccgtggcct ggtgtcgcga cccgacctcg agacggtgct cgactatcgc gactggatcg
     6601 acctgcgcgt gctcgcgttg ctcgcggacc cggcggaagg ctcgtgccat gtcgaagtga
     6661 tcgaacgggg cctgcagcac gagcagcgcc accaggagcg cctgctcgcc gacgtcaagc
     6721 acctgctcgc ctttcatccc gctgcgccgg cctattaccc gcgcccggcg gccgtgcccg
     6781 ggcccgacgc ggccgccggc acgccgctcc gcatggtgga aatcgaaggc ggactgcagt
     6841 ggctcggcgc cggcgaggcc ggcttcgcgt tcgcgcagga gcggccgcgt catcgccatt
     6901 ggctcgagcc ctgcgcgctc ggcgagcggc tggtgaccaa cggcgagtat cgcgccttcg
     6961 tcgacgcggg cggttacgag cgcgcggaat tctggtccgt ggaaggctgg cgcgcgaggg
     7021 tcgcgcaggg ctggcgcgcc ccgctgtact ggtcgctgga agaaccggcg atgcacttta
     7081 cgctcggcgg gctgcagccg ctggacgctc tcgcgccagt ggtgcacgtg agccggcacg
     7141 aggccgaggc gtacgcgcgc ttcgccggtg cgcgcctgcc gaccgaggcg gaatgggagt
     7201 gcgccgcccg ccgcgtgccg ctgcacggac cgttcctgga atctggaact tttgcgccat
     7261 tcgcggctcc gacgacaccg tcgcccctgc tgcaacaggt gtacggcgtt gcctggcagt
     7321 ggacccgctc cgccgccacg ccatatccgg gcgcacacga cgacggcccg cacgcgagcc
     7381 gcgcgtcggg cgtacggtac gactcgacgc agggcgtggc ccgcggcggc tcgtgcataa
     7441 cgccgagcgg gcacgtgcgc ccctcgtctc gtctgttcct gaacccggat gcgcggtggc
     7501 agttcaccgg gatccggctc gccaaggatt gccccgcctc atgagaaacg ccatcgacgt
     7561 cgcgcggcac acgcgcgtcg caccgccgca cgcggcccgg ccgctgatcg cactcgtctt
     7621 cgagggactc agcgaagagc agaagcagct gccgtcgatg ctggcgttcg atccggtggg
     7681 ctcgcagctg ttcgaacgcg tctgcgagca gccggagtat ttcctcgcgc gggccgaagc
     7741 cacgctgttg cgccggcacg cggcggaaat cggcgccgtg gtcggtccgc aggccgccat
     7801 cgtcgagtac ggggccggcg ccggacggct gggcgcactg ctgctcgacg ccatgacgtc
     7861 cgcccattcg tacgtgccga tcgacgtcga cgcgacacaa ctcgcccgct gccgggaagc
     7921 cattcgcgcg agtgggcgcg cgctgcgcat ccatcccctg caccaggact tccgccagtt
     7981 cgtcgctctg ccgacgacgc tcggcggggc gcgacgacgc gtggcctact tcccgggctc
     8041 gagcgtcggc tcgttccggc aactcgaggc ggtggcgctg ctcaactcca tgcgcgagac
     8101 cgtgggcccc ggcggcggcc tgctcgtggg catcgacctg ctcaaggaac cgcaggtgca
     8161 cctgcgttcc tggctcgacg cggccggaac cgtcgccgcg ttcaacggca acgtgctgct
     8221 gcggctcaac cgcgagatcg acgcgacgtt cgaccctgcc ggcttcgagc atcgcgttgc
     8281 ctggaacgag tcggagcggc gcctggaaat gggactcgtg agcctgcgcg cgcaggcacc
     8341 gaccatcgcc ggcataggcg tcgcgctcgc ggccggcgag gagatccgga cgtcggtggc
     8401 gcacaagtac tcgctcgagg agtttgcgtc gctggcgcac atcgcgggct gggttgcccg
     8461 ctcgacctgg gtcgacaccg aacacgacta cgcgctgcag tacctcgagg ccggcgaata
     8521 accttcatcg ccgacggcga tgccccggga gaagatcgcc gggcacgggt gc
//
LOCUS       DASPFR010000028         5847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1231129, whole genome shotgun sequence.
ACCESSION   DASPFR010000028 DASPFR010000000
VERSION     DASPFR010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5847
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231129"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..269)
                     /locus_tag="VD737_00695"
     tRNA            complement(194..269)
                     /locus_tag="VD737_00695"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(234..236),aa:Phe,seq:gaa)
     gene            392..1012
                     /locus_tag="VD737_00700"
     CDS             392..1012
                     /locus_tag="VD737_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXW09106.1"
                     /translation="MGRRAVFDRNQMTGAALRLVAERGPQAVTVSALAQEVGAPTGSI
                     YHRYRSREQLLAELWMEVVEGFQNRFVESLAQARDVEGAISTARLMVAWTREHPLEAR
                     LLLLHRRQDFVSGEWPAELAQRAAALEPQLGTALRAFADRAFGRADAETMARLRYALL
                     DAPFGALRPYVQGQKRLPAVVDELVIATARAVLASAVRPGNPKASS"
     gene            1012..1956
                     /locus_tag="VD737_00705"
     CDS             1012..1956
                     /locus_tag="VD737_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09107.1"
                     /translation="MSAPFVDRSDGAPLLAGATQLEFHRPPSASRYMFRAVLPTSRER
                     NLAPRIVARWIGCRPSAQEVAEFRRITGADARDDDPTALPFLYPLTVGFRLSMAVLTH
                     PRFPVPIWGVLQTRNHVTQHRRIPTGASLDFETHVLPARVVSKGAEFDLRTNVHVDGD
                     LAWESVVTFFARGRFGEPDAASTSARSPSDVGDAIAEWTMADADHARFCRFTGDYNGI
                     HLWDWYARRMGFRRALYHPQRVLGLCLARLPAPGLRTTSPAGEKLDAWIKGPVPHGAR
                     VRLQASTSPTSTTFALFADDGARPAIVGRLHATEPDRP"
     gene            1953..2333
                     /locus_tag="VD737_00710"
     CDS             1953..2333
                     /locus_tag="VD737_00710"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXW09108.1"
                     /translation="MTLTTAARDLLVADFLAAWDSQDVERLLDCYTDDLRYRDPNTRG
                     EVVGRDALRRYLTRLFAEWRMTWESRETFTFDGADGAAFLWRARLRRTGSDRTVEIEG
                     MDLAVLRGDKVARNEVYFDRSLVA"
     gene            complement(2349..2612)
                     /locus_tag="VD737_00715"
     CDS             complement(2349..2612)
                     /locus_tag="VD737_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HXW09109.1"
                     /translation="MATGWAGDGAVQQQIDDSIEDAIKRARAQLPAGPSLTHCEECGE
                     EIPAPRREAVPGVRLCVECQAERDQSSALALYNRRGSKDSQLR"
     gene            complement(2699..3280)
                     /locus_tag="VD737_00720"
     CDS             complement(2699..3280)
                     /locus_tag="VD737_00720"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HXW09110.1"
                     /translation="MKTVARVWITILAGVALAVVASMQPWQRSTARAASDRPAPELPE
                     LPASAWLNSSPLTLAEQRGRPVLVEFWTFGCSNCRATLPWLKRVHERYAPQGLQVISI
                     HSPEFEHERDPTAVARHVRQFGIRYPVLIDNEFRYWKALDNRFWPAFFLIDPQGRLVD
                     RRIGELHPGERSADAFERRIASYLPGDANHSGK"
     gene            complement(3430..4251)
                     /locus_tag="VD737_00725"
     CDS             complement(3430..4251)
                     /locus_tag="VD737_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXW09111.1"
                     /translation="MDISATDKTFAGGIPEIYERHMVPLFFEPYARDLAARLAPLGAQ
                     RVLELAAGTGVLTRHLARVLDPRASLVATDLNPQMIEQAKQRGTDRAVDWQVADALSL
                     PFEDGSFDLVVCQFGAMFFPDKGQAWMEARRVLRPGGRLVFNVWDRIERNGFDDTVQR
                     AVAALYERDPPSFMARVPHGYYDEDTIRADLRRGGFTVDAAIETLELPTVAPSPRDPA
                     VAICQGTPMRHEIEAKGAGEVLRATEAAAAAVLARFGQRDLVAESRALLVSVVTG"
     gene            complement(4306..5181)
                     /locus_tag="VD737_00730"
     CDS             complement(4306..5181)
                     /locus_tag="VD737_00730"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HXW09112.1"
                     /translation="MSQHHPVIAVTGSSGAGTSTVMKSFAHIFRREGIEAQVVEGDSF
                     HRYDRVEMRARVKAADSGEGPQISHFGPESNLLAELAQTFEHYGRSGHGKVRRYVHDE
                     AEAKELGSPPGTFTEWQPMAPRSDLLFYEGLHGGYVGPGVDVAKHVDLLVGVVPIINL
                     EWIQKLHRDKHQRGYSQEAVVDTILRRMPDYVNHICPQFSRTHINFQRVPTVDTSNPF
                     VAKDIPTADESMVIIRFANPKGIDFPYLLSMLDGSWMTRPNSIVAPGGKMGLAMQLIL
                     TPMILRLVDRKRRAA"
     gene            complement(5261..>5847)
                     /locus_tag="VD737_00735"
     CDS             complement(5261..>5847)
                     /locus_tag="VD737_00735"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF040139.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HXW09113.1"
                     /translation="MLVLTVGKGTHGFTLDREIGNFILTHPDLRIPADTSEFAINTSN
                     ARFWEAPVHRYVTECQAGRVGERGRDFNMRWIASLVAEVHRILVRGGVFMYPRDTRDP
                     ERPGRLRLLYEANPISFLIEQAGGASSTGRQRVLDLRPHVLHQRVPVIFGSRSEVERI
                     ARYHWEHDQGFDRRYSSPLFNERSLFRHESAGAS"
BASE COUNT          895 a         2022 c         1966 g          964 t
ORIGIN      
        1 agcaccatgc ttctcggcga tctcgttccg tctcagtact acgggacctg caagaagtcg
       61 taggctggta acttctcgtc cgccaacggg gtgtccatca cggggacggc tgccctgcat
      121 cgaacgatgg aaacaaaatg gaaacttcgc ccgtccaggc cgaatatggt cgcgcttaag
      181 ttgttgattt aaatggtggc cagggacgga atcgaaccat cgacacgcgg attttcagtc
      241 cgctgctcta ccaactgagc tacctggcca cggcatgacc cggcgctgac ggccggcctg
      301 cagggagccg cgaattagac ccgagcgccc cgaacccgtc aatctgtcaa gtcctcttga
      361 cgccacgttc tagaatgtac attctaaaaa aatgggccgt cgggcagtct tcgatcgcaa
      421 ccagatgacg ggcgccgccc tgcgcctggt cgccgagcgc ggcccgcagg cggtgacggt
      481 ctcggccctt gcgcaggaag tcggcgcgcc gacgggctcc atctatcacc gttaccgcag
      541 tcgcgagcaa ctgctggccg agctctggat ggaggtcgtc gagggtttcc agaatcggtt
      601 cgtcgagagt ctcgcccagg cgcgcgacgt cgaaggcgcg atttccaccg ctcgcctgat
      661 ggtcgcctgg acgcgcgagc acccgctcga ggcgcgcctg ctgctgctgc accgtcggca
      721 ggacttcgtc agcggagaat ggccggcgga gcttgcgcag cgagccgctg cgctcgagcc
      781 gcagttgggc accgcgttac gggccttcgc cgatcgcgcg ttcgggcgtg ccgatgccga
      841 gacgatggcg cgcctgcgtt acgcattgct cgacgcaccg ttcggcgcct tgcgccccta
      901 cgtgcagggc cagaagcgcc tgcctgcggt cgtcgacgaa ctggtcatcg cgacggcgcg
      961 cgccgtcctg gcgtcggcgg tgcgtcctgg aaaccccaag gcatcctcgt gatgagcgct
     1021 cccttcgttg accgaagcga cggcgcgccc ctgctcgcag gcgcgacgca gctcgagttc
     1081 catcggccgc cgtcggcatc gcgatacatg tttcgcgcgg tcctgccgac gtcgcgcgag
     1141 cgcaatctcg cgccgcggat cgtcgcgcgc tggatcggct gccgcccatc cgcgcaggag
     1201 gtggccgagt ttcgccgcat caccggggcc gacgcccgcg atgacgaccc gacggcgctg
     1261 cccttccttt atccgctcac ggtcggcttc cggctgtcca tggccgtcct gacgcatccg
     1321 cgctttcccg tgccgatctg gggcgtgctg cagacgcgca atcacgtgac ccagcatcgc
     1381 cggatcccga ccggcgcttc cctcgatttc gagacgcacg tgctgccggc ccgcgtcgtg
     1441 tcgaaaggcg cggagttcga cctgcgcacg aacgtgcacg tcgacggaga cttggcctgg
     1501 gaaagcgtcg tcacgttctt cgcgcgcgga cgcttcggcg agcccgacgc cgcatcaacc
     1561 tccgcgcgct caccgagcga tgtcggagac gcgatcgccg aatggacgat ggccgacgcc
     1621 gatcacgcgc gcttttgtcg attcaccggt gactacaacg gcatccacct gtgggactgg
     1681 tacgcgcggc gcatgggatt ccgtcgcgcg ctgtaccacc cgcagcgcgt gctgggcctg
     1741 tgcctcgcgc ggttgcccgc ccccgggctg cggacgacct cgcccgcggg cgagaaactc
     1801 gacgcctgga tcaagggccc ggtgccgcac ggcgcgcgcg tgcgcctgca ggcctcgacc
     1861 agccccacgt ccacgacctt cgcgctgttc gccgacgacg gagcgcgtcc ggccatcgtc
     1921 ggccgcctcc acgccacgga gcccgatcga ccatgacgct cacgaccgct gcccgcgacc
     1981 tgctcgtcgc ggatttcctc gccgcctggg actcgcagga cgtcgaacgg ctgctcgact
     2041 gctacacgga cgacttgcga taccgcgatc cgaacacgcg cggcgaagtc gtcggccgcg
     2101 acgccttgcg acgctacctg acgcgcctgt tcgccgagtg gcggatgacc tgggagtcgc
     2161 gcgaaacgtt cacgttcgac ggcgcggacg gcgcggcctt cctgtggcgc gccaggctgc
     2221 gtcgaaccgg cagcgaccgc acggtcgaga tcgaagggat ggacctggcc gtgctgcgcg
     2281 gcgacaaggt cgcccgcaac gaggtctact tcgatcgatc gctggtcgcc tgaaccaccg
     2341 gggaaccgtc agcgcaactg gctgtctttg gagccgcgcc ggttgtagag cgccaatgct
     2401 gacgactggt cacgctcggc ctggcactcc acgcataagc gcacgccggg cacggcctcg
     2461 cgacgcggcg cggggatctc ctcgccacac tcctcgcagt gcgtcaggct cgggcccgcg
     2521 ggcaactgcg cgcgggcgcg cttgatcgcg tcctcgatgc tgtcgtcgat ctgctgctgc
     2581 acggcgccgt cgccggccca tcccgtcgcc atcgtcgctt cctgtctcta ctgccgaatg
     2641 actcaaagtt ggaccggatc atgcccgctg cgttcccggg cctgaagggg acgcttcgct
     2701 attttccgct atggttggcg tcccccggga ggtacgacgc aatccgacgc tcgaacgcgt
     2761 ccgccgagcg ctctcccggg tgcaattcac cgatccgccg gtcgacgagg cgtccctgcg
     2821 ggtcgatcag gaagaacgcg ggccagaagc ggttgtcgag tgccttccag taccggaact
     2881 cgttgtcgat cagcacgggg taacgaatgc cgaactgccg gacgtgacgc gccactgccg
     2941 tcgggtcgcg ctcgtgctcg aactccggag agtgtatcga gatcacctgc aggccttgcg
     3001 gtgcgtaccg ctcgtgaacg cgcttgagcc agggcagcgt cgcgcgacag ttcgagcaac
     3061 cgaaggtcca gaactccacc agcaccggcc ggccccgctg ctcggcgagc gtgagaggcg
     3121 acgagttgag ccatgcgctg gccggcagct cgggcagttc cggcgcggga cggtcgctgg
     3181 cagcccgcgc ggtcgaacgc tgccagggtt gcatcgaggc tacgacggcg agcgcaaccc
     3241 ccgcgaggat cgtgatccag actcgtgcga cggtcttcac ggcgacattt cctcatcgac
     3301 ggcgctcgca aagcacgccc tgaccctcag tagaccgccc ggcctcttcg aacattacgg
     3361 cgcgagccgg gcgggcggtc ggcgacctcg tgcaggtcgg tctgccgcga aggcggcggc
     3421 gacgttgcct cagccggtca cgaccgagac gagcaaggca cgcgactcgg cgacgaggtc
     3481 acgctggccg aaccgcgcga ggacggccgc ggcggccgct tcggtcgcgc gcaggacctc
     3541 gccggcgccc ttcgcctcga tctcgtgacg cattggcgtg ccctggcaga tggccacggc
     3601 cgggtcgcgt ggggacggcg cgaccgtcgg caactcgagc gtctcgatcg ccgcatccac
     3661 cgtgaagccg cctcgccgca ggtcggcccg gatcgtgtcc tcgtcgtaat acccgtgcgg
     3721 tacccgggcc atgaagctgg gcggatcccg ttcatagagc gcggccaccg cgcgctgcac
     3781 ggtgtcgtcg aatccgttgc gctcgatccg gtcccagacg ttgaacacga gtcgaccgcc
     3841 cggacgcagc actcgtctcg cctccatcca cgcctggcct ttgtccggga agaacatcgc
     3901 gccgaactgg cagacgacga ggtcgaacga gccgtcctcg aacggtagtg aaagtgcgtc
     3961 ggcgacctgc cagtccacgg cacggtccgt gccgcgctgc ttcgcctgct cgatcatctg
     4021 ggggttgagg tccgtcgcca cgagcgacgc gcgagggtcg aggacgcgcg cgaggtgccg
     4081 cgtcagcacc cccgtgccgg ccgcgagctc gagcacgcgt tgcgccccca gcggcgccag
     4141 gcgcgcggcg aggtcacgcg catacggctc gaagaacagc ggcaccatgt gccgctcgta
     4201 gatttcgggg atgccgccgg cgaacgtctt gtcggtcgcg gagatgtcca tcaggccctc
     4261 acccgcgcta cgcgcgacct ctcccgcgag cgggagaggt gaagactacg ccgcgcggcg
     4321 cttgcggtcg acgagccgca ggatcatcgg cgtcaggatc agctgcatcg cgagccccat
     4381 cttgccgccc ggcgcaacga tgctgttggg gcgcgtcatc cacgagccgt cgagcatcga
     4441 cagcaggtac gggaagtcga tgcccttcgg gttggcgaag cggatgatca ccatcgactc
     4501 gtccgcggtc gggatgtcct tggcgacgaa cgggttgctc gtgtcgacgg tcggcacgcg
     4561 ctggaagttg atgtgcgtgc ggctgaactg cgggcagatg tggttcacgt agtccggcat
     4621 acgacgcagg atcgtgtcga cgacggcttc ctggctgtag ccgcgctggt gcttgtcgcg
     4681 gtgcagcttc tggatccact cgaggttgat gatcggcacc acgccgacca gcaggtcgac
     4741 gtgcttcgcc acgtccacgc cgggccccac gtaaccgccg tgcaggcctt cgtagaacag
     4801 caggtcgctg cgcggtgcca tcggctgcca ctcggtgaac gtgccgggcg gcgagccgag
     4861 ctccttcgcc tcggcctcat cgtggacgta gcgtcgcacc ttgccgtggc cgctgcggcc
     4921 atagtgctcg aacgtctgcg cgagctcggc gagcaggttc gactccggcc cgaagtggct
     4981 gatctgcggg ccctcgcccg agtcggccgc cttgacgcgc gcgcgcatct cgacgcggtc
     5041 gtagcggtgg aacgagtcgc cttcgaccac ctgcgcctcg atgccttcac gccggaagat
     5101 gtgcgcgaac gacttcatga cggtggacgt gccggcgccc gaagagccgg tgaccgcgat
     5161 gacgggatgg tgctgcgaca tggtcgtctc tctgctctgc tgtgttctca cctctcccgc
     5221 ttgtgggaga ggtcgcgcaa agcgcgggtg aggggtcgag tcagctcgcc cccgccgatt
     5281 cgtgccggaa cagcgacctc tcgttgaaga gcggcgacga gtagcggcga tcgaacccct
     5341 ggtcgtgctc ccagtgataa cgcgcgatgc gctcgacctc gctgcgcgat ccgaagatca
     5401 cgggcacgcg ctggtgcagc acgtgcggtc gcaggtcgag cacccgctgc cggccggtcg
     5461 acgaggcgcc gcccgcctgc tcgatgagga agctgatcgg gttggcctcg tacagcagtc
     5521 gcaggcgccc gggccgttcg ggatcacgcg tgtcgcgcgg gtacatgaac acgccgccgc
     5581 ggacgaggat gcggtgcacc tcggcgacca gcgaagcgat ccagcgcatg ttgaagtcgc
     5641 ggccgcgctc ccccactcgt cccgcctggc attcggtgac gtatcgatgc accggcgcct
     5701 cccagaaccg cgcgttgctc gtgttgatcg cgaactcgct cgtgtcggcc gggatgcgca
     5761 ggtccggatg cgtcaggatg aagttgccga tctcgcggtc gagcgtgaag ccgtgcgtgc
     5821 ccttgccgac ggtcagcacc agcatcg
//
LOCUS       DASPFR010000029         3136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_376806, whole genome shotgun sequence.
ACCESSION   DASPFR010000029 DASPFR010000000
VERSION     DASPFR010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376806"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VD737_00740"
     CDS             <1..504
                     /locus_tag="VD737_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HXW09114.1"
                     /translation="TLPRFLYALGIRDVGEATALALAEHFGDLDALMQASLEEIQQVR
                     DVGPVVAGHVREFFDEERNRKVVAQLRAAGVTWPRLTRSVAGGPGPMAGKVVVITGTL
                     VSMSREEAREAARAAGATVTDSVSKKTSLLVVGAEAGSKLKKAQDLGVAIVDEAAFLK
                     MLGRGGA"
     gene            560..1483
                     /locus_tag="VD737_00745"
     CDS             560..1483
                     /locus_tag="VD737_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXW09115.1"
                     /translation="MLRYALGRLLGIVPTLFVIVTAAFFVMRLAPGGPFDEEQAMPPE
                     IAANLEAAYGLDQPMLVQYGRYVSGLLRGDLGPSFKYKDYRVAELIGRGLPVTVTVGV
                     LALILAIAAGVPLGLAAALRHDGPVDHAVMGVALFGIAVPNFVLAPVLALVLGVHLQW
                     LPVAGWEPGSVRHLVLPVVTLALPFVAYVARLTRGSLLDVLQAPYIRTARAKGLARGA
                     LLRRHALKPTLLPVVSYLGPAAAALLTGSLVVEQVFGLPGVGRYFVQGAINRDYTLVM
                     GMVIVYAALILLLNLAVDLFYGWLDPRIRHG"
     gene            1476..2366
                     /locus_tag="VD737_00750"
     CDS             1476..2366
                     /locus_tag="VD737_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXW09116.1"
                     /translation="MVEATVDVVPHRRAGLWSDAWSRLRRSRAAWSAALVLGAIVLAA
                     LVGPALSPYTMAQVDWTQAALASPPSLDTGHWFGTDANGRDLFVRLWVGTRMSLLVAV
                     LATLVSVVIGVAWGATAGYLGGRVDDWMMRFVDVLYALPYMFFVIILTVVFGRSLVLI
                     FLAIGAVGWLTMARIVRGQALALRHREFVEAAIALGLPARTVIFRHIVPNVSGTVIVY
                     ATLTVPQVILFESFLSFLGLGVQEPLTSLGRLVAEGALEMETSPWLLLIPSAVLGTLL
                     LCLNFLGDGLRDALDPRERR"
     gene            2363..>3136
                     /locus_tag="VD737_00755"
     CDS             2363..>3136
                     /locus_tag="VD737_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXW09117.1"
                     /translation="MTQASTLLRVEGLEVRFDTGHGVVQAVAGVSLDVRAGECVAVVG
                     ESGSGKTQLLLACLGLLAQNGQAAGSARFEGQQLIGTSDAVLNTVRGTGIALLSQDPL
                     SALTPHLRIETQLVELLRDRGRAGKAELRQRALAALRDVEIPDPESRLRQYPHELSGG
                     MRQRVALAMALMGGPRLLFADEPTTALDVSVQARILELLARVRDRGLGILIITHDLGV
                     VAGLADRVAVMYAGRIVEHAPVRRLFTAPAHPYTAALLAA"
BASE COUNT          392 a         1086 c         1126 g          532 t
ORIGIN      
        1 acgctgccgc ggtttctcta tgcgctcggc atccgcgacg tgggcgaggc cacggcgctc
       61 gcgctcgcgg agcatttcgg cgacctggac gcgctcatgc aggcgagcct cgaggagatc
      121 cagcaggtcc gcgacgtcgg accggtggtg gccggccacg tacgcgagtt cttcgacgag
      181 gagcgcaacc gcaaggtcgt ggcgcagttg cgcgcggccg gcgtcacctg gcccaggctc
      241 acacgcagcg tcgccggcgg cccgggaccg atggcgggca aggtggtcgt gattacgggc
      301 acgctcgtgt cgatgtcgcg agaagaggcg agggaggctg cgcgcgccgc cggcgcgacc
      361 gtgacggact cggtgtcgaa gaagacgtca ctgctggtgg tcggcgccga ggccggatcc
      421 aagctcaaga aagcgcagga cctcggcgtg gcgatcgtcg acgaggcggc gttcttgaag
      481 atgctcggac ggggcggggc gtgacccttg ccctcacccg gcctgcggcc gacctctccc
      541 gcgtgcggga gaggtgatca tgctgcgata cgcgctcggc cggctgctcg gcatcgtgcc
      601 gacgctgttc gtgatcgtca cggcggcctt cttcgtgatg cgcctcgcgc cgggcggtcc
      661 gttcgacgag gagcaggcga tgccgccgga gatcgcggcg aacctagagg cggcctatgg
      721 cctcgaccag cccatgctgg tccagtacgg tcgctacgtc tccgggctgc tccgtggcga
      781 cctcggtccg tcgttcaagt acaaggacta ccgcgtcgcc gagttgatcg gccgcggctt
      841 gccggtgacg gtgacggtcg gcgtgctggc gctgatcctg gcgatcgcgg caggcgtgcc
      901 gctcggcctg gccgccgcgc tcaggcacga cgggcccgtc gatcacgccg tgatgggcgt
      961 ggcgctgttc ggcatcgccg ttcccaactt cgtgctcgca ccggtgctgg cgctcgtgct
     1021 gggcgtacac ctgcaatggc tgcccgtcgc cggctgggag cccggcagcg tgcgtcacct
     1081 ggtcctgccg gtcgtgacgc tggcgctgcc gttcgtcgcc tacgtcgcac ggctgacgcg
     1141 gggcagcctg ctcgacgtct tgcaggcgcc ctacatccgc acggcgcgcg cgaaggggct
     1201 ggcgcgaggc gcgctgctgc gccggcacgc actcaagccg acgctgttgc cggtcgtgag
     1261 ctacctcgga cctgccgcag ccgcgctgct caccggttcg ctcgtcgtcg agcaggtctt
     1321 cggcctgccg ggcgtcggcc gctacttcgt gcagggcgcg ataaaccgcg actacacgct
     1381 cgtgatgggc atggtgatcg tctacgcggc gctgatcctg ctgctcaatc tcgccgtcga
     1441 cctgttctac ggctggctcg acccgcggat ccgccatggt tgaggcaacc gtcgacgtcg
     1501 tgccgcaccg tcgtgcgggc ctctggtcgg acgcgtggtc ccgcctcagg cgcagccgcg
     1561 cggcctggag cgccgcactc gtgctcggcg cgatcgtgct cgcggcgctc gtcggtccgg
     1621 cgctcagtcc ctacacgatg gcgcaggtcg actggacgca ggcggcactc gcgtcgccac
     1681 cgtcgctcga caccggccac tggttcggca ccgacgcgaa cggccgcgac ctcttcgtgc
     1741 gcctgtgggt cggcacgcgc atgtcgctgc tggtggcggt gctcgcgacg ctggtcagcg
     1801 tcgtgatcgg cgtcgcctgg ggtgccacgg ccgggtacct cggcggccgc gtcgacgact
     1861 ggatgatgcg cttcgtcgac gtgctctacg cgctgccgta catgttcttc gtgatcatcc
     1921 tgaccgtggt gttcggacgc agcctcgtgc tgatcttcct cgcgatcggc gcggttggct
     1981 ggctcacgat ggcgcgcatc gtgcgcggcc aggcgctcgc gttgcgtcac cgcgagttcg
     2041 tcgaggccgc gatcgcgctc ggcctgccgg cgcgcacggt catcttccgg cacatcgtgc
     2101 cgaacgtgtc cgggaccgtg atcgtctacg cgacgctgac ggtgccgcag gtgatcctgt
     2161 tcgagagctt cctgagcttc ctcggcctcg gcgtgcagga accgctcacc agcctcggac
     2221 gcctggtcgc cgaaggcgcg ctcgagatgg aaacctcgcc gtggctgctg ctgatcccgt
     2281 cggccgtgct gggcacgctc ctgctgtgcc tgaacttcct gggcgacggg ctccgcgacg
     2341 cactcgaccc acgggagcgt cgatgacgca ggcctccacg ttgctgcggg tcgagggtct
     2401 cgaggtgcgc ttcgacaccg gccacggcgt cgttcaggcg gtcgccggcg tctcgctcga
     2461 cgtgcgcgcg ggcgagtgcg tggccgtcgt tggcgagtcg ggctccggca agacgcaact
     2521 gctgctcgcc tgtctcgggc tgctcgcgca gaacggccag gccgccggtt cggcacggtt
     2581 cgaagggcag caactgatcg gcacgagcga cgccgtcctg aatacggtcc gcggcaccgg
     2641 catcgccttg ctgtcgcagg acccgctcag tgcgctgacg ccgcacctgc gcatcgagac
     2701 gcaactggtc gagctgctgc gggaccgcgg ccgtgcaggc aaggccgagt tacggcaacg
     2761 cgcgctcgcc gcgctgcgcg acgtcgaaat ccccgatccg gagtcgcgat tgcgccagta
     2821 tccgcacgag ctgtccggcg gcatgcgcca gcgcgtggcg ctcgcgatgg cactgatggg
     2881 cggcccgcgc ctgctgttcg cggacgagcc caccacggcg ctcgacgtca gcgtgcaagc
     2941 gcgaatcctg gaattgttgg cgcgcgtccg tgaccggggc ttgggaatcc tgatcatcac
     3001 gcacgacctg ggcgtcgtgg cgggactcgc cgaccgcgtc gcggtgatgt acgccggccg
     3061 gatcgtcgag cacgcgcccg ttcgtcggtt gttcacagcg ccagcgcacc cgtacacagc
     3121 cgcgctgctc gcggca
//
LOCUS       DASPFR010000030         8576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1476642, whole genome shotgun sequence.
ACCESSION   DASPFR010000030 DASPFR010000000
VERSION     DASPFR010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476642"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2334
                     /gene="metH"
                     /locus_tag="VD737_00760"
     CDS             <1..2334
                     /gene="metH"
                     /locus_tag="VD737_00760"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HXW09118.1"
                     /translation="SISLKEGERPFLAQARKVRAYGAAVVVMAFDEQGQADTVERKVG
                     ICQRSYRLLTEQVGFPPEDIVFDPNIFAVATGIEQHDRYALDFIEATARIREACPYAL
                     VSGGVSNVSFSFRGNDPVREAMHSVFLYHAIRAGLSMGIVNAGQLAIYEEIEPELRER
                     VEDVILARREDATERLLEAAARYRGEAGQKRAEDLAWRTWPVEKRLEHALVKGIDEFI
                     LEDTDAARLELGRPLQVIEGPLMNGMNVVGDLFGVGKMFLPQVVKSARVMKKAVAHLI
                     PWLEKEQDGTSRSNGKVVMATVKGDVHDIGKNIVGVVLQCNNFEVIDLGVMVPADRIL
                     ETAEREGASMIGLSGLITPSLDEMVHVAREMKRRGFGLPLLIGGATTSPAHTSVKIDP
                     EYDGPVVYVKDASRAVGVCQSLVTSEGREDYVARLAHDHSARREQHKGRKVKGPALSL
                     AEARANRYTCDWQAYRPPVPKLTGVRVLENVPLDELVRYIDWMPFFNAWEFAGKFPDV
                     LTDPVVGEAASNLYADARRVLKQIIAERWVRASAVLGLFPANSDGDDVEVYADETRTR
                     IVQRLHTLRQQKPKPAGQPHYALADFVAPKGGGVRDWVGAFAVTAGLGLDEKVREFEA
                     SHDDYGAIMLKALADRLAEALAERMHERVRREFWGYAPEERFTSDQLIREDYRGIRPA
                     PGYPACPDHTEKATLWRLLDVEAQTGIRLTESYAMHPTAAVSGWYFSHPEARYFQVGK
                     IDVDQMADYARRKNWSFAEAERWLAPLVGYDTPASAA"
     gene            complement(2374..5934)
                     /locus_tag="VD737_00765"
     CDS             complement(2374..5934)
                     /locus_tag="VD737_00765"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF025376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HXW09119.1"
                     /translation="MRGTPALSISSRALATPEPFVRLFARRVQQEGALDLTQGDYKNA
                     DFAPHPEVVRAAQRITRNTVHSYGPAVGRMDVRNEVAEFFNRDGLIDYPGTEVRFLPD
                     EVLFTPGTRAGLAIVLEVLGGDGTGVVVPRPSWEYDWFIERAGKKVVELPTAAPEFLP
                     DPADLDRLLARGGISCLILNNPHNPTGRVYPRELVEALVATAVRHRCYVLYDSVYQRL
                     DYVDWFVNPAFASPEWRDYVVSLSGLSKMDMFGASTGARACWLVISDQIRTNGIRARE
                     ILANLSAWLVATPSTLAQDWALAALQSPLAALRRPSPYMRERRDFMLKAADALAPLGI
                     ERTDFGGTFYAPLAFPGLVGEPFDRLRNGVHERAVVRDSVDAFEFLLSGGVGGIPFVA
                     FAGGAQGADRYGTWQRLSYGSKDVKELAVFMDRVRARIEQQGRLGAAATVPTSPSTND
                     AVWQSACTADGYTALDALEPGAFAAARRRFLENPRHEDLRLTGTKHPDSTTHRAEQLE
                     RALRASAAVGGPDQPHAALTHIEWNPLMEARLEYEETITDLLGPCSEVLLDLEGRQIS
                     PDWLDVPEKVVLALLRHGVVPGEDRHLLVRVPNPFLEQDEEKVSKILASVARSNLLFL
                     LACERLGLAPKQNAICEISVPQVNSTAEIGAVTKVGTMYLQAIDGLFEGTGDRVDAFL
                     SARIPAGRRNDLRGLVSRVRLVPLCENVGALARLPDLLEQFYLALERGFGLEGVPTPA
                     TFCTTFDGPEATVRVFVAMSDTALQSGKLATDAAYTLAAAGRDEAERRLALRAARSGQ
                     PAPRVTLLIGAGRAGFRGGFDPRHPGVIRQFARADGVTLQGIRADSPEGTLELAAAFR
                     AAVAAQRGRRDPLPRADADSIAKLMYEGVRAHTETLLRIAPLLAPFGSLVPQTRVRIR
                     ATGSANYGRSIPTYPPEWGGGTVLPDDRDLRDAWPEGVTLPRAIVYNLACTTLGLPAV
                     TSDLGVLDRRAAVLLDRHVPGYREIVASELPAFVKEAAALVFGRKLAETTARRCLRAA
                     AALWVDARGRPDLVAPTCLFAMQYLRYMAEDSESWDETKEHESRTTREEELVRETSSV
                     AFVALCAERPGDRWPVLQSMVDAEDAPRLLLARELTLEEKREFVDLRIEGWLRSLPEP
                     LSRELRPEWARLRELPRAEFELDAIRRLIELEKLRGSCGA"
     gene            6322..7413
                     /locus_tag="VD737_00770"
     CDS             6322..7413
                     /locus_tag="VD737_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXW09120.1"
                     /translation="MEVKTMSLRRRASVVAAMGFALWSAGSSAAVTLRVDAQPVADPI
                     QVFVNVTSAAGAPVTGLQAGDFAVRVDGTTVSSPTFSLPPASGGGKVSVVLAMDMSQS
                     VQNAALESMQQAVIGFLDSMQNGDYAAIVKFNNTNPDKASLVQDFTPIDGGAGNSALE
                     SAVMAPYPGSGSNVLDGVTLAINTLGSPSVALPSGPKAIVLVSDGRDNASTATLDTVV
                     SAATAASIPVFTIGVGDLTTTGGRLLSDLATRTGAEYIPAPDNGDIAEAYARISQRLG
                     NEYVLTFNSSITDCNSHSVQVDVAGNGSATSQFQRCTDSGNPPPPPGGGGDGGGGGGG
                     GGASGLLEVALGLSLVALARRRRFGRNLR"
     gene            complement(7490..>8576)
                     /locus_tag="VD737_00775"
     CDS             complement(7490..>8576)
                     /locus_tag="VD737_00775"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXW09121.1"
                     /translation="EAARRGAIAALIVHETEPASYGWATAIAPNGEGYDIVRADPARE
                     KLLLQSWIHRDAAVQLLRRCGLDLEALKARARSRAFEPVTLRDATFSADFALAHSRIV
                     SHNVIGRITGGRYPAESIMIGAHWDAYGIGPADAQGQRLRPGALDDAIGVAGAIEIAR
                     AFGRGPRPERTLLFAAWTAEERGLLGSEYYAAHPWLPLETTVANLTMDVLQPNGPARD
                     VVLVGAGQNELEALLAAKAADQDRVVTPDAHPERGLAFRADHFSFARRGVPALLLMGI
                     GGGHDLVTGGREAGDRWVSDFTARCYHQACDRWSDEWDLRGAAQDVALVFEVARDLAS
                     SGAWPEWNAGSEFKSVRDSSAARRSSR"
BASE COUNT         1311 a         2973 c         3047 g         1245 t
ORIGIN      
        1 tcgatcagcc tcaaggaagg cgagcgtccg ttcctcgcgc aggcgcgcaa ggtgcgcgcg
       61 tacggcgcgg cggtcgtcgt gatggcgttc gacgagcagg gccaggccga caccgtcgag
      121 cgcaaggtcg ggatctgcca gcgctcctac cggctgctga ccgaacaggt cggtttcccg
      181 cccgaggaca tcgtcttcga ccccaacatc ttcgcggtcg cgaccggcat cgagcagcac
      241 gaccgctacg cgctcgactt catcgaggcg accgcgcgga tccgcgaggc ctgcccgtac
      301 gcgctcgtga gcggcggcgt cagcaacgtg tcgttctcgt tccgtggcaa cgacccggtg
      361 cgcgaggcga tgcactcggt cttcctgtac cacgcgatcc gcgcgggatt gagcatgggc
      421 atcgtcaacg cgggccagct cgcgatctac gaggagatcg agccggagct gcgcgagcgg
      481 gtagaggacg tgatcctggc gcggcgcgag gacgcgaccg aacgcctgct cgaggccgcc
      541 gcgcgttacc ggggcgaggc gggccagaag cgcgcagagg acctcgcgtg gcggacctgg
      601 cccgtcgaga agcgactcga gcacgcgctg gtcaagggca tcgacgagtt catcctcgag
      661 gacaccgacg ccgcgaggct ggaactcggc cgcccgctgc aggtgatcga agggccgctg
      721 atgaacggca tgaacgttgt cggcgacctg ttcggcgtcg gcaagatgtt cctgccgcag
      781 gtcgtgaagt cggcgcgcgt catgaagaag gcggtcgcgc acctgatccc gtggctcgag
      841 aaggagcagg acggcacgag ccggtcgaac ggcaaggtgg tgatggccac cgtcaagggc
      901 gacgtgcacg acatcggcaa gaacatcgtc ggcgtggtgc tccagtgcaa caacttcgag
      961 gtgatcgacc tcggcgtgat ggtgccggcg gaccgcatcc tcgagacggc cgagcgcgag
     1021 ggcgccagca tgatcggcct ttcgggactg atcacgccct cgctcgacga gatggtgcac
     1081 gtggcccgcg agatgaagcg ccgaggcttc gggctgccgc ttttgatcgg cggcgcgacg
     1141 acctccccgg cgcacacgtc cgtcaagatc gatccggagt acgacggccc ggtcgtgtac
     1201 gtcaaggacg cttcgcgggc ggtgggtgtc tgccagtcgc tggtcacgag cgaaggtcgc
     1261 gaggactacg tcgcgcgcct cgcccacgac cactcggcgc ggcgggagca gcacaagggc
     1321 cgcaaggtga aggggccggc gctctcgctc gccgaagccc gggccaaccg ttacacgtgc
     1381 gactggcagg cataccgacc cccggtcccg aagctgacgg gtgtccgggt cctcgagaac
     1441 gtgcctctcg acgaactcgt gcggtacatc gactggatgc cgttcttcaa cgcctgggaa
     1501 ttcgcgggca agtttcccga cgtgctgacc gaccccgtcg tcggcgaggc cgcgagcaac
     1561 ctctatgccg acgcccggcg cgtgctcaag cagatcatcg ccgagcgctg ggtgcgcgcg
     1621 agcgcggtgc tcgggctgtt cccggccaac tcggacggcg acgacgtcga ggtgtacgcc
     1681 gacgagacgc gcacgcgcat cgtgcagcgg ctgcacacgc tgcggcaaca gaagcccaag
     1741 ccggccggcc agccacacta cgcactcgcg gacttcgtcg ctccgaaggg cggcggcgtg
     1801 cgcgactggg tgggtgcctt cgccgtgacg gccgggctcg gcctggacga gaaagtccgc
     1861 gagttcgagg caagccacga cgactacggc gcgatcatgc tcaaggcgct cgccgaccgc
     1921 ctcgccgagg cgctcgccga acgcatgcac gagcgggtgc ggcgtgagtt ctggggctac
     1981 gcgcccgagg agcgcttcac cagcgatcag ctgatccgcg aggactaccg cggcatccgt
     2041 ccggcgccgg ggtacccggc gtgtcccgac cacaccgaga aggccacgct gtggcgcctg
     2101 ctcgacgtcg aagcgcagac gggcatccgc ctgacagaga gctacgcgat gcaccccacg
     2161 gcggcggtga gtggctggta cttctcgcac cctgaagcgc ggtatttcca ggtgggcaag
     2221 atcgatgtgg accagatggc cgactacgcg cggcgcaaga actggtcgtt cgccgaggcc
     2281 gagcgctggc tcgcgccgct cgtcggctac gacacgcccg cgagcgcggc ctgagccgga
     2341 gcgccgcctc gcggcgccgc gggcggtcgc cgactacgcg ccgcagctgc cgcgcagctt
     2401 ctcgagctcg atcaagcggc gtatggcgtc gagctcgaac tctgcccgcg gcagttcgcg
     2461 caggcgcgcc cactcgggcc gcagttcgcg cgacaacggc tcgggcaggc tgcgcagcca
     2521 gccctcgatg cgcaggtcga cgaactcgcg cttttcctcg agcgtgagtt cacgcgccag
     2581 cagcaggcgg ggcgcgtcct cggcgtccac catcgactgc agcaccggcc agcggtcgcc
     2641 cgggcgctcg gcgcagagcg cgacgaaggc gacgctcgag gtctcacgga ccagttcctc
     2701 ctcgcgcgtg gtgcggctct cgtgttcctt ggtctcgtcc cagctttcgg agtcctcggc
     2761 catgtagcgc aggtactgca tcgcgaagag gcaggtcggg gccacgaggt ccgggcggcc
     2821 gcgcgcgtcg acccagagcg ccgcggctgc gcgcaggcag cgccgcgcgg tggtctcggc
     2881 gagcttgcgg ccgaagacca gcgccgcggc ttccttgacg aacgccggga gctcgctcgc
     2941 gacgatttcg cgatagcccg gcacgtgacg gtcgagcagc acggcggccc tgcgatcgag
     3001 caccccgagg tcgctcgtca ccgcgggcaa ccccagcgtg gtgcaggcca ggttgtagac
     3061 gatcgcccgc ggcagcgtca caccttccgg ccacgcgtcg cgcaggtcgc ggtcgtccgg
     3121 gagcaccgtg ccgccgcccc attccggcgg gtacgtcggg atcgagcggc cgtagttggc
     3181 cgaacccgtc gcgcggatgc gcacgcgcgt ctgcggcacg aggctgccga acggtgccag
     3241 cagcggcgcg atgcgcagca gcgtctcggt gtgcgccctg acgccctcgt acatgagctt
     3301 cgcgatggag tcggcgtccg cgcgcggcag cgggtcgcga cgcccgcgct gggctgcgac
     3361 ggccgcacga aaggcggcgg cgagttcgag cgttccctcg ggggagtcgg cacggatgcc
     3421 ttgcagcgtc actccgtcgg cgcgggcgaa ctggcggatc acgccggggt gccgcgggtc
     3481 gaacccgcca cggaagccgg cacgcccggc accgatcagc aacgtcacgc gcggcgcggg
     3541 ctgtccggag cgggcagcgc gcagcgccag gcggcgctcc gcctcgtccc ggccggccgc
     3601 tgcgagcgta taggcggcgt cggtcgccag ctttccgctc tgcagcgcgg tgtcggacat
     3661 cgcgacgaac acgcgcacgg tcgcctcggg accgtcgaac gtcgtgcaga acgtggccgg
     3721 cgtcggcacg ccctccaggc cgaagccacg ttcgagcgcg aggtagaact gctcgagcag
     3781 gtcgggcagg cgagccagcg cgccgacgtt ctcgcacagc ggcacgagtc gcacacggct
     3841 cacgagcccg cgcaagtcgt tgcgacgccc ggccgggatg cgagccgaca ggaaggcgtc
     3901 gacccggtcg cccgtgccct cgaacaaccc gtcgatcgcc tgcaggtaca tcgtgccgac
     3961 cttggtcacc gccccgatct cggcggtcga gttgacctgc ggcacgctga tctcgcagat
     4021 cgcgttctgc ttgggcgcga ggccgaggcg ctcgcaggcc aggaggaaca gcaggttgct
     4081 ccgcgctacc gacgcgagga tcttcgagac cttctcctcg tcctgctcaa ggaacgggtt
     4141 cggcactcgc acgagcaggt ggcggtcctc gccgggcacg accccgtggc gcagcagcgc
     4201 gagcacgacc ttctcgggca cgtcgagcca gtcggggctg atctgccggc cttcgaggtc
     4261 gagcagcacc tcgctgcacg ggccgagcag gtcggtgatc gtctcctcgt actcgagccg
     4321 cgcctccatg agcgggttcc actcgatgtg ggtcagggct gcgtgcggct ggtcggggcc
     4381 gccgacggcc gcggacgcgc gcagcgcgcg ctcgagttgc tcggcgcgat gggtggtgga
     4441 gtcggggtgc ttggttccgg tgagtcgcag gtcctcgtga cgaggattct cgaggaagcg
     4501 acggcgggcg gcggcgaagg cgcccggttc gagcgcgtct agcgcggtgt agccgtcggc
     4561 cgtgcaggcg ctctgccaga ccgcgtcatt ggtcgatggc gatgtcggca ccgtcgccgc
     4621 cgcgccgagt cgaccctgct gctcgatccg ggcgcggacc cggtccatga acacggccag
     4681 ctccttgacg tccttggagc cgtaggaaag gcgctgccac gtgccatacc ggtcggcacc
     4741 ctgcgcaccg ccggcgaacg cgacgaacgg gatgccgccc acgccgcccg acagcaggaa
     4801 ctcgaaggcg tccacggaat cgcggacgac cgcacgttcg tggacgccgt tgcgcagccg
     4861 gtcgaacggt tcgccgacca ggcccggaaa ggcgagcggg gcgtagaacg tcccgccgaa
     4921 atcggtgcgc tcgatgccga gcggcgcgag cgcgtccgcc gccttcagca tgaagtccct
     4981 gcgctcgcgc atgtacggag agggcctgcg cagagccgca agcgggctct gcagggccgc
     5041 gagcgcccag tcctgcgcga gcgtcgaggg cgtcgcgacg agccaggccg agaggttggc
     5101 caggatctcg cgcgcccgga tgccgttcgt gcggatctgg tcggagatca ccagccagca
     5161 ggcgcgcgcg ccggtcgacg cgccgaacat gtccatcttc gaaaggccgg acagcgacac
     5221 gacgtagtcg cgccattctg gcgacgcgaa cgccggattg acgaaccagt cgacgtagtc
     5281 gagccgctgg tacacagagt cgtacagcac gtagcagcga tgccgcacgg cggtcgccac
     5341 cagtgcctcc accagctcgc gcggatagac gcggccggtc gggttgtgcg ggttgttgag
     5401 gatcaggcac gaaattcccc cacgcgcgag cagcctgtcg aggtcggccg ggtcgggcag
     5461 gaactcgggc gcggccgtcg gcagttcgac gaccttcttg ccggcacgtt cgatgaacca
     5521 gtcgtattcc cagctcggcc gcggtacgac gacgccggtg ccgtcgccgc cgagcacctc
     5581 gagcacgatg gccaggccgg cccgggtgcc cggggtgaac agcacctcgt cgggcaggaa
     5641 gcggacctcg gtgcccgggt agtcgatcag gccgtcgcgg ttgaagaatt ctgcgacctc
     5701 gttgcgcacg tccatgcgac cgaccgcggg cccgtagctg tgcacggtgt tgcgcgtgat
     5761 gcgctgtgcc gcgcgcacga cttccggatg aggcgcgaag tccgcgttct tgtagtcgcc
     5821 ctgcgtgagg tcgagcgcgc cttcctgctg gactcggcgg gcgaacaggc ggacgaacgg
     5881 ctcgggcgtc gcgagcgcgc gcgaggaaat gctcagggcg ggggtgccgc gcatcgggga
     5941 gacctgcgcc aggagggcgc aagtaccgag attcttcatt gttcgcgcgc cggctgcagt
     6001 cgtacttacc accgtcgcgg actgccacgg cgcgcgtttg acgcttcttt cacgtcgtac
     6061 ggtcggacgg tggcggtgaa accgactgtc ggcctgtagg ttttcgttcc gtgcttcgtc
     6121 ggcctgcggc tgtaagccaa atcggtcaga cagaaggagg ttaaaccggc acggctcgct
     6181 ccgcctgatc cctccgcgcc gcgcttcgtt ggggccacca tggcgcccgg acgtcaaccg
     6241 cgcgagctcc atcgaccggc ccacgcatct tcgtgcagcc agcgtcgcgg gcagtgcagc
     6301 gcaaccacgg aagaggaact catggaagtc aaaacgatgt cgctccggcg cagagcgtca
     6361 gtggtcgccg cgatgggctt cgcgctctgg tccgccggat ccagtgcggc cgtcacgctg
     6421 cgggtcgatg cgcagccggt ggccgatccc atccaggtat tcgtcaacgt gacctctgca
     6481 gcgggcgcgc cggtgaccgg cctgcaggcc ggcgattttg cggtgcgcgt ggacggcacc
     6541 acggtttcgt cgcccacctt ctcgctgccc ccggcctcgg gaggcggcaa ggtgtccgtg
     6601 gtactggcga tggacatgag ccagtccgtc cagaatgccg ccctcgagtc gatgcagcag
     6661 gcagtcatcg gcttcctcga ctcgatgcag aacggcgatt acgccgcgat cgtgaagttc
     6721 aacaacacga acccggacaa agcgtcgctg gtccaggatt tcacgccgat cgacggcggc
     6781 gccggcaact ccgccctcga gtccgccgtg atggcgccgt accccggatc cggcagcaac
     6841 gtgctcgacg gcgtgacgct ggccatcaac acactcgggt caccgtccgt ggcgctgccg
     6901 agcggtccga aggcgatcgt gctggtcagt gacggtcgcg acaacgcctc gacggccacg
     6961 ctcgacacgg tcgtctcggc ggcgacggcg gccagcatcc cggtcttcac gatcggtgtc
     7021 ggtgacctga cgacgaccgg cgggcggctg ctgtcggacc tcgccacacg gaccggcgcg
     7081 gaatacatcc cggcgcccga caacggcgac atcgcggagg cgtacgcgag gatctcgcag
     7141 cggctcggca acgagtacgt gctgaccttc aattccagca tcaccgactg caactcgcac
     7201 tccgtgcagg tggacgtagc cggcaacggt tctgcgacga gccagttcca gcgctgcacc
     7261 gacagtggca atccgccgcc gcccccgggc ggtggtggtg acggcggtgg tggtggcggc
     7321 ggcggcggcg cctcgggact gctcgaggtc gccctgggtc tttcgctcgt cgcgctggcc
     7381 cgccgccgac gcttcggacg caacctgcgc tgatctgccg cgcgttcccg gcacgctcag
     7441 gccccttccg cgaggaaggg gcctttccac gcgcaatgga tccctgtgct catcgagacg
     7501 agcgccgcgc ggcgctcgaa tcgcgcacgg acttgaactc cgaccctgcg ttccactcag
     7561 gccacgcccc cgaactggcg aggtcgcggg ccacctcgaa gacgagcgcg acgtcctgcg
     7621 ccgcaccgcg caggtcccat tcgtcgctcc agcgatcgca ggcctggtga tagcaccgcg
     7681 cggtgaagtc gctcacccag cgatcgccag cctcacgccc gcccgtcacc aggtcgtgcc
     7741 cgccgccgat gcccatcagc agcagcgccg gcacgccgcg gcgcgcaaac gaaaagtggt
     7801 cggcgcggaa cgcgagcccg cgctccgggt gcgcgtcggg ggtcacgacg cggtcctggt
     7861 ccgcagcctt cgcggcgagc agcgcctcca gctcgttctg cccggcgccg accagcacga
     7921 cgtcgcgcgc gggtccgttc ggttgcagca cgtccatcgt caggttggcc accgtcgtct
     7981 cgagcggcag ccacggatgc gccgcgtagt attccgagcc cagcaggccg cgttcctcgg
     8041 ctgtccaggc cgcgaacagg agcgtccgtt cgggcctcgg cccgcggccg aaggcccgcg
     8101 caatctcgat cgcgccggcg acgccgatcg cgtcgtcgag tgcgcctggc cggaggcgct
     8161 gaccctgcgc gtccgccgga ccgatgccgt aagcgtccca gtgcgcgccg atcatgatgc
     8221 tttcggcggg gtacctgcca cccgtgatcc tgccgatcac gttgtggctc acgatgcgcg
     8281 agtgcgcgag cgcgaagtcg gccgagaagg tcgcgtcacg caacgtgacg ggctcgaagg
     8341 cgcgactgcg ggcgcgcgcc ttgagcgcct cgaggtcgag cccgcagcgt cgcagcagct
     8401 gcaccgcggc gtcgcggtgg atccacgact gcagcagcag cttctcgcgc gcagggtcgg
     8461 cacgcacgat gtcgtagcct tcgccgttcg gtgcgattgc ggtcgcccag ccgtacgaag
     8521 ccggctcggt ctcgtgcacg atcagggccg cgatggcacc gcgccgcgcc gcctct
//
LOCUS       DASPFR010000031         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_866460, whole genome shotgun sequence.
ACCESSION   DASPFR010000031 DASPFR010000000
VERSION     DASPFR010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866460"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..903)
                     /locus_tag="VD737_00780"
     CDS             complement(193..903)
                     /locus_tag="VD737_00780"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXW09122.1"
                     /translation="MPEAAPADRVVVVTGAFGALGTAVARAFGQAGARLGLIDLAQPS
                     VELQSEFAGHLLLGGVDLANEEATRSTMAAIAMRFGGIDVLVNVAGGFRWEKVEGGTL
                     DTWDALYAMNLRSAVVSCKCALPALLERGAGRIVNVGAGAAAAKAGTGMGAYTASKAG
                     VQRLTEALAEEVKDRRVTVNAVLPGTIDTPSNRLDMPDADFSRWVPPEQIARVIVFLA
                     SEDADAITGASIPVFGRG"
     gene            complement(1025..2395)
                     /locus_tag="VD737_00785"
     CDS             complement(1025..2395)
                     /locus_tag="VD737_00785"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HXW09123.1"
                     /translation="MNLSVLDIFKIGVGPSSSHTMGPMNAACAFVRDLEAQGLLERTE
                     KLVAQMYGSLALTGRGHCTDRAVLLGLEGNEPSTIDPAAMEPALERIRSTGRLRLGGR
                     REIPFDEPLDLLFHRDQILPQHPNGMRFTALDSNGEVLHRDEYFSTGGGFVVRATDFG
                     KPASGTGQVQVRYPFGSAEELLELAREHGLELHELVLANERAFRPDQETRAALLRVWT
                     VMSECIDRGFHGTGVLPGVLRVKRRAARLHRQLVESGPARPLDALEWVNAYALAVNEE
                     NAAGGRVVTAPTNGAAGIVPAVLAYYRKYEPGAGEDGVVRFLLVAGAIGMLYKRNASI
                     SGAEMGCQGEVGVACSMAAAGLVAALNGTNEQIENAAEIGMEHNLGLTCDPIAGLVQI
                     PCIERNAMGAVKAINAARLSLRGDGTHKVTLDQVIATMRQTGHDMSTIYKETSQGGLA
                     VNVPEC"
BASE COUNT          411 a          930 c          833 g          428 t
ORIGIN      
        1 gcagttcgac tacccgcgca acctcgcgca cgactccgag tgaaacaggg acactcctga
       61 ttcacggcgg tagatgggac accccgcggt tcccagtaaa tggggagtaa atggggacac
      121 tccgaatttc ccgataaatc aggaatgtcc ccatttactg ccgtaaatca ggagtgtccc
      181 tattttctgg ggtcagccac ggccgaacac ggggatcgag gcgccggtga tcgcgtccgc
      241 atcttccgag gccaggaaga cgatcacgcg cgcgatctgc tccggcggta cccagcggct
      301 gaagtctgcg tcgggcatgt cgagccggtt gcttggcgtg tcgatcgtgc cgggcaggac
      361 cgcattgacg gtgacgcggc ggtccttgac ttcctcggcc agcgcctcgg tcaggcgctg
      421 cacgccagcc ttcgacgccg tgtaagcgcc catccccgtc cctgccttcg ctgccgccgc
      481 gccggccccg acgttgacga tccggcccgc gccgcgctcg agcaacgcgg gcagcgcgca
      541 cttgcagctc accaccgccg accgcaggtt catcgcgtac agagcatccc aggtgtcgag
      601 cgtgccgccc tcgacctttt cccagcggaa gccgcctgcg acgttcacca gcacgtcgat
      661 gccgccgaag cgcatcgcga tcgcggccat cgtactgcgc gtcgcctctt cgttggcgag
      721 gtccacgccg cccagcagca gatgccccgc gaactcgctc tgcaactcga ccgatggctg
      781 cgcgaggtcg atcagcccca gccgcgcgcc tgcctgtcca aacgcccgcg ccaccgcggt
      841 acccagcgca ccgaacgcac cggtgacgac gacgacacga tccgcgggtg cagcttcagg
      901 catccgattc ctctccgcac gagtggctag tggctagtgg ctagtggtca gtgaaggaca
      961 ggagggtgca tcgaccgtcg tttcgtcacc agccactgac cactaaccac tgaccacttc
     1021 tccctcagca ttccggcacg ttcaccgcca gcccgccctg gctcgtttcc ttgtagatgg
     1081 tcgacatgtc gtggccggtc tggcgcatgg tggcaatcac ctggtcgagt gtcaccttgt
     1141 gcgtgccgtc gccacgcagc gacaggcgcg cggcgttgat ggccttgacg gcgcccatcg
     1201 cgttgcgctc gatgcaggga atctggacga gcccggcgat cgggtcgcac gtgaggccga
     1261 ggttgtgctc catgccgatc tcggcggcgt tctcgatctg ctcgttcgtg ccgttgagcg
     1321 ccgcgacgag cccggccgcc gccatcgagc acgcgacccc gacctcgccc tggcatccca
     1381 tctcggcacc ggagatcgag gcgttgcgct tgtagagcat gccgatcgcc ccggcgacga
     1441 gcaggaaccg cacgacgccg tcctcgcccg cgcccggttc gtacttgcgg tagtaggcga
     1501 gcacggcggg cacgatgccc gcggcgccgt tggtcggcgc cgtcacgacg cggccgcctg
     1561 cggcattctc ctcgttgacg gcgagcgcgt aggcattgac ccactcgagg gcgtcgagcg
     1621 gccgagccgg ccccgactcc acgagctgcc ggtgcaaccg cgccgcacgc cgcttcaccc
     1681 tgagcacgcc cggcaggact ccggtgccgt gaaagccccg gtcgatgcac tcgctcatca
     1741 cggtccagac ccgcagcagc gccgcgcggg tctcctggtc cggccggaaa gcgcgctcgt
     1801 tcgcgagcac cagctcgtgc agctcgagac catgctcgcg cgcgagctcg aggagctcct
     1861 ctgccgagcc gaacggatac cgtacctgca cctgcccggt gcccgatgcc ggcttgccga
     1921 aatcggtggc gcgcacgacg aaaccgccac cggtcgagaa gtactcgtcg cggtgcagga
     1981 cctcgccgtt cgaatcaagc gccgtgaacc gcatgccgtt cgggtgttgc ggcaggatct
     2041 ggtcgcggtg gaacaacagg tcgagcggct cgtcgaatgg gatctcgcgg cgaccgccga
     2101 gccggagccg gcccgtgctg cggatgcgct cgagcgcggg ctccatcgcg gccgggtcga
     2161 tcgtcgacgg ttcgttgccc tcgagcccca gcagcaccgc acgatcggtg caatgcccgc
     2221 ggcccgtcaa cgcgagcgac ccgtacatct gcgccacgag cttttcggtc cgttcgagca
     2281 gaccctgcgc ttcgaggtcg cgcacgaacg cgcaggccgc gttcatcggc cccatcgtgt
     2341 gggagctcga cggcccgacc ccgatcttga agatgtccag tacgcttagg ttcaagtggc
     2401 tagtggctag gggccagtgg ctagtgtccg gagacgcggc gcagccgcct ccccactgaa
     2461 caccagccac taaccactag ccacttcctc ctttcattcc atggccagca gcgacgcgtt
     2521 gccgccgacg gccgacgtgt tgacggtgta cgtgcgctcg tgcgcgaacc ggtgcaggta
     2581 gtgcgggccg ccggccttgg gg
//
LOCUS       DASPFR010000032         5133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_500138, whole genome shotgun sequence.
ACCESSION   DASPFR010000032 DASPFR010000000
VERSION     DASPFR010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500138"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VD737_00790"
     CDS             complement(<1..256)
                     /locus_tag="VD737_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09124.1"
                     /translation="MTAPRRRLGWIIYATALTLAVLIGEVSRGTRPDLVALASWILTA
                     ALLAAVWSYALRHRLGHEGYWRVVFWIVSFATALMLVPVLV"
     gene            complement(268..471)
                     /locus_tag="VD737_00795"
     CDS             complement(268..471)
                     /locus_tag="VD737_00795"
                     /inference="COORDINATES: protein motif:HMM:NF016028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HXW09125.1"
                     /translation="MSEDGVERLEFKIAHLEKAVQELSDVVYRQQRELDALRERGRAL
                     LEQVQQLEDRGADPNRVEIPPHY"
     gene            complement(468..1166)
                     /locus_tag="VD737_00800"
     CDS             complement(468..1166)
                     /locus_tag="VD737_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXW09126.1"
                     /translation="MYELYIANKNYSSWSLRPWVLMKTLGIPFVEHVVPFAPGSNFEA
                     FRAFSPTGKVPCLRDGELVVWDSLGIACYLAERHAGLWPEEVAARTWARCATAEMHSG
                     FAALRNHCGMNLGVRVRLRAWPPELQADVRRLDELWSEGLDRFGGTFLAGATFGIVDA
                     FFCPVAYRARTYDIPLGRTARGYAERLLELPAMREWEAAGLAEPWRDEAHEAEIAQYG
                     DVVADLREPAAARA"
     gene            complement(1168..2301)
                     /locus_tag="VD737_00805"
     CDS             complement(1168..2301)
                     /locus_tag="VD737_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09127.1"
                     /translation="MLLSTSPVEPRAPRILFVPVSGPGGSGELMRCLIVARELRRACP
                     DADVHFLVARNAVFRETVDFPIHDCDDSPTRSTPQVLATLRALAPDVVVFDNAGRTAQ
                     LRAAKALGARLVFTSRSPRLRWKAFRLSWLRLLDEHWIVFPQFVTGGLSPLERLKLHL
                     SPNYVVRHLDTLFTPSSPDERQRFLAAHGLRAGGYTAFVPGGRGEGRTATRVEPADLF
                     IEAAAQFTRDTGQTSVVLTGRDRPTRPADASERLVLLPRLRPEDVQHLLAGAARVVSN
                     GGTTLVHALAYGQEIVCAPLAADQARRIRRAARLGVVVPVAPVAGPIARATAELAADR
                     ARGEMLRRRVVELGLVNGVNEAVAALQRLARPASSTRLSLQEA"
     gene            complement(2362..2784)
                     /locus_tag="VD737_00810"
     CDS             complement(2362..2784)
                     /locus_tag="VD737_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HXW09128.1"
                     /translation="MDDLDRQLIGLLRSNARLPVATLAKRLRVARGTVQNRLSRLERD
                     GVIAGYTLRLRAPDEERRVVALMLLAVEGNRVEDVLVALRGDPAVSALHTTNGRWDIV
                     AELRADTLEEFDRVLGRIRRIEGVATSETNLLLSTLKY"
     gene            2949..4040
                     /locus_tag="VD737_00815"
     CDS             2949..4040
                     /locus_tag="VD737_00815"
                     /inference="COORDINATES: protein motif:HMM:NF005762.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HXW09129.1"
                     /translation="MVEYIGVGDVERLVNALGPAEFMARLAAEIEADYRRWPEFDNAP
                     RLASHSPVGVIELMPATDGRLYSFKYVNGHPKNTAEGLLTVMAFGVLAEVDTGYPLLL
                     SELTVTTALRTAATSALAARHLARPDSRVMALIGNGAQSEFQAIAFHRLVGINELRLY
                     DVDARASAKLEQNLAAMPGLENLRVIRCRSSAEACRGADIVTTVTADKRNAVILTPPM
                     IVPGMHLNAVGGDCPGKTELHPDILRRPDLRVVVEFEPQSRIEGEIQQMPPDYPVIEF
                     ASVLRGEATARRSPTDVTLFDSVGFALEDYSALRFLYELVCEQQLGRRSLDLVPMLDD
                     PKDLFGGTLGGGHAHSRARVRRTARGSVS"
     gene            4037..4987
                     /gene="rocF"
                     /locus_tag="VD737_00820"
     CDS             4037..4987
                     /gene="rocF"
                     /locus_tag="VD737_00820"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HXW09130.1"
                     /translation="MTSRHVALIGAPTDIGAGTRGASMGPEALRVAQLQPVLESQGVV
                     VHDRGNLAGPANPWQPPVDGYRHLREVVEWNRLVHDAVHAELGGGRLPILLGGDHCLA
                     IGSISAAARYCRATGRKLRVLWFDAHADFNTSVLTPSGNIHGMPVACLCGHGPAELTG
                     LGGVVPAIEPSQLCQIGIRSVDAGEKRFVHEVGYEVFDMRYIDEMGMRYVLEHALAGA
                     DETTHIHVSLDVDFLDPDIAPGVGTTVPGGPTYREAQLCMEMVADTGRLGSLDIVELN
                     PAFDVRNRTAEVAVDLIGSLFGKSTLMRKEPANQWPVASG"
BASE COUNT          791 a         1805 c         1783 g          754 t
ORIGIN      
        1 acaccagcac cggcaccagc atcagcgcag tcgcgaacga cacgatccag aacaccacgc
       61 gccagtaacc ctcgtgaccg agccggtgtc gcagcgcgta cgaccacacg gccgccagca
      121 gggccgccgt caggatccag ctcgcgagcg cgacgaggtc gggacgcgtg ccacgcgaca
      181 cttcgccgat cagcacggcg agcgtcagcg cggtggcgta gatgatccag cccaggcgtc
      241 gtcggggcgc tgtcaacgcg ggcgcgctca gtagtgcggc gggatctcga cgcggttggg
      301 gtccgcgccg cggtcctcga gctgctgcac ctgctcgagt agcgcacggc cgcgctcgcg
      361 caaggcgtcg agctcacgct gctgtcgata tacgacgtcg ctcaattcct gcaccgcctt
      421 ctcgaggtgc gcgatcttga actcgaggcg ctccacgccg tcctcgctca tgcgcgggcc
      481 gctgcgggct cgcgcaggtc cgccacgacg tcaccgtact gcgcgatttc ggcttcgtgc
      541 gcctcgtcac gccagggttc cgcgagcccc gccgcttccc actcgcgcat cgcgggcagt
      601 tccagcagtc gctcggcgta accgcgcgca gtgcgtccca ggggaatgtc gtaggtgcgc
      661 gcgcggtacg ccacgggaca gaagaacgcg tcgacgatgc cgaacgtcgc accggcgagg
      721 aacgttccgc cgaagcggtc caggccttcg ctccagagtt cgtcgagccg gcgcacgtcc
      781 gcctgcagtt ccggcggcca cgctcgcagg cgcacccgga cgccgaggtt catgccgcag
      841 tggttgcgca gcgccgcgaa tcccgagtgc atctcggccg tcgcgcagcg cgcccacgtg
      901 cgagctgcga cctcctccgg ccagagcccg gcgtgccgtt ccgcgaggta acacgcgatg
      961 ccgagcgagt cccagacgac cagctcgccg tcccgcaggc acggcacctt gccggtcggc
     1021 gaaaacgccc ggaacgcctc gaagttcgag cccggcgcga acggcacgac gtgctcgacg
     1081 aacgggatgc cgagcgtctt catgagcacc cagggccgca gcgaccagga cgagtagttc
     1141 ttgttggcga tgtagagctc gtacacctca ggcctcctgc agcgacaacc gtgtcgagga
     1201 cgccgggcgt gccagccgct gcagcgccgc gaccgcttcg ttcacgccgt tgacgagacc
     1261 gagctcgacg acccgccggc gcagcatctc gccgcgggca cggtccgccg cgagttcggc
     1321 ggtcgcgcgt gcgatcgggc cggcgacggg cgcgaccggc acgacgacgc cgagccgcgc
     1381 ggcgcgcctg atccgccgtg cctggtccgc cgcgagcggc gcgcagacga tctcctgccc
     1441 gtaggcgagc gcgtggacca gcgtggtgcc gccattgctg acgacgcgcg cggcgccggc
     1501 gagcaggtgc tgcacgtctt ccggccgcag ccgcggcagc agcaccaggc gctcgctcgc
     1561 gtccgcggga cgggttgggc ggtcacgacc ggtcagcacg acgctcgtct gcccggtatc
     1621 gcgcgtgaat tgcgcggctg cctcgatgaa gaggtccgcc ggctcgactc gcgtcgcggt
     1681 ccgcccttcg ccgcgtccac cgggcacgaa cgccgtgtag ccgcccgcgc gcaggccgtg
     1741 cgcggcgagg aagcgctggc gctcgtccgg gctcgacggc gtgaacagcg tgtcgaggtg
     1801 gcgcacgacg taattggggg agaggtgcag cttgagccgc tcgagcggcg agagaccgcc
     1861 cgtgacgaac tgcggaaaca cgatccagtg ctcgtcgagc agccgcagcc acgacagccg
     1921 gaacgccttc cagcgcagcc gcggcgagcg gctcgtgaag acgagccggg cgccgagcgc
     1981 cttggcggcg cgcagctgcg ccgtgcggcc ggcgttgtcg aacacgacca cgtccggtgc
     2041 gagcgcacgc agcgtcgcga gcacctgcgg cgtgctgcgc gtgggcgagt cgtcgcaatc
     2101 gtggatcggg aaatcgacgg tctcacggaa cacggcgttg cgcgccacga ggaaatggac
     2161 gtcggcgtcc gggcacgcgc gtcgcaactc gcgcgcgacg atcaggcaac gcatcagctc
     2221 gcccgatccg ccgggcccgg acacgggcac gaacaggatg cggggagcac gcggctcgac
     2281 tggagaagtg gaaagcagca tcaccggggt ggcgggatcg ttccggccgg ggcctggggc
     2341 atgatacggc acgtcaccga ttcaatactt gagcgtcgag agcagcaggt tggtctcgga
     2401 ggtcgcgacg ccctcgatgc gacggatgcg accgagcacg cgatcgaact cctccagggt
     2461 gtcggcacgc aattccgcga cgatgtccca gcgcccgttg gtggtgtgca gcgcagagac
     2521 ggccgggtcg ccgcgcaagg cgacgaggac gtcctcgacg cgattgcctt cgaccgcgag
     2581 cagcatcagc gcgacgaccc gacgctcctc gtccggagcg cgcagccgca gcgtgtagcc
     2641 cgcgatcact ccgtcgcgct cgagtcgcga caggcggttc tgcaccgtgc cgcgcgccac
     2701 gcgcaggcgc ttggcgagcg tcgccacggg cagccttgcg ttgcttctca gcaggccgat
     2761 cagctggcgg tcgaggtcgt ccatcgcagc tctggacttg gcactctgct cgctctcgat
     2821 gacgatatgc ccgacttata gcacatatag gtcgtttcgc tgccttcctg ttggctcggg
     2881 gagccgcgat gatggctgcg aggccacaca cggaagaacg ccgcggcccc ggcagcagga
     2941 gcattcggat ggtcgagtac atcggggtgg gcgacgtcga gcgcctggta aatgcgctcg
     3001 gccccgccga gttcatggcg cgtctcgccg cggagatcga ggccgattac cgtcgctggc
     3061 ccgagttcga caacgcgccg cggctggcca gccattcccc ggtcggcgtc atcgagctga
     3121 tgccggccac ggacggacgg ctctattcgt tcaagtacgt caatggccat ccgaagaaca
     3181 ccgcggaagg gctgctgacg gtcatggcgt tcggcgtgct cgcggaggtc gacaccggct
     3241 atccgctgct gctctccgag ctgacggtca cgacggcgct gcggacggcg gcgacgtcgg
     3301 cgctggccgc gcggcatctc gcgcgacccg acagccgcgt catggcgctg atcggcaacg
     3361 gggcgcagag cgagttccag gcgatcgcgt tccaccgcct ggtcggcatc aacgaactgc
     3421 ggctctacga cgtcgatgcg cgcgcctccg cgaagctcga gcagaacctc gccgccatgc
     3481 cgggcctcga gaacctgcgc gtcatccggt gtcgctcgtc ggcggaggcc tgtcgcggcg
     3541 cggacatcgt caccaccgtg acggcggaca agcgcaatgc cgtgatcctg acgcccccca
     3601 tgatcgtccc cggcatgcac ctgaacgcgg tcggcggcga ttgcccgggc aagaccgagc
     3661 tgcacccgga catcctgcgc cgccccgacc tccgcgtggt ggtcgagttc gagccgcaat
     3721 cgcgcatcga gggcgagatc cagcagatgc ccccggacta ccccgtgatc gagttcgcga
     3781 gcgtcctgcg gggcgaggca acggcgcgtc gttcgccgac cgacgtcacg ctgttcgact
     3841 cggtcggctt cgcgctcgag gactactccg cgttgcgttt cctgtacgaa ctcgtgtgcg
     3901 aacaacagct cggtcgccgt tcgctcgacc tcgtgccgat gctcgacgat ccgaaggacc
     3961 tgttcggtgg cacgctcggc ggcggtcacg cacactcgcg tgcgcgcgtc cgtcgcacgg
     4021 ctcgagggag cgtttcatga cgagcaggca cgtcgcgctg atcggcgccc cgaccgacat
     4081 cggcgcaggc acgcgaggcg cctcgatggg gccggaggcc ctgcgcgtcg cccagctgca
     4141 gcccgtgctc gagtcgcagg gggtggtggt gcacgaccgc ggcaacctgg ccgggcctgc
     4201 caatccgtgg cagccgccgg tcgacggcta tcggcacctg cgcgaagtcg tcgagtggaa
     4261 ccggctcgtg cacgacgccg tgcatgccga actgggcggg ggccgcctgc cgatcctgct
     4321 gggcggcgac cactgcctgg cgatcggctc gatcagcgct gcggcacgct attgccgcgc
     4381 gaccggtcgc aagctgcgcg tgctgtggtt cgatgcccac gccgacttca acacgagcgt
     4441 gctgacgccg agcggcaaca tccacggcat gcccgtcgcg tgcctctgcg ggcacgggcc
     4501 ggccgagctg accggactgg gcggggtggt gcctgccatc gagccctcgc agctgtgcca
     4561 gatcgggatc cgcagcgtgg acgccggcga gaagcgcttc gtccatgaag tgggctacga
     4621 ggtgttcgac atgcgctaca tcgacgagat gggcatgcgt tacgtgctcg agcacgcgct
     4681 cgcgggcgcg gacgagacga cgcacatcca cgtgagcctc gacgtcgact tcctcgaccc
     4741 cgacatcgcg cccggcgtcg gcacgaccgt gcccggcggt ccgacctacc gcgaagcgca
     4801 gctctgcatg gagatggtcg cggacacggg acgcctgggc tcgctcgaca tcgtcgagct
     4861 caatcccgcg ttcgacgtgc gcaaccgcac ggccgaagtg gccgtcgacc tgatcgggtc
     4921 gctgttcggg aagagcacgt tgatgcggaa agagccagcc aaccagtggc cagtggctag
     4981 tggctagtgg ctagtgcagg agagacgcgt cacgtcggcc gctcttcact agccactgac
     5041 cactgaccac tagccactgc ctttcttccc ctccttccgt cgcgcttccg tcgtctcgcg
     5101 cttgcgctgg tctccgccgg cgtcgtcctt gtc
//
LOCUS       DASPFR010000033         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1599211, whole genome shotgun sequence.
ACCESSION   DASPFR010000033 DASPFR010000000
VERSION     DASPFR010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599211"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /gene="pyrB"
                     /locus_tag="VD737_00825"
     CDS             <1..688
                     /gene="pyrB"
                     /locus_tag="VD737_00825"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF012412.4"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HXW09131.1"
                     /translation="YTLQAMGVDVFVMRDSEPGVPAFVAGHVQPHASVLNAGEAHVSH
                     PTQGLLDVLTIRQRKGKDLGSLSVAIVGDIRHSRVARSTGTALQTLGVGDLRLVSPPS
                     LAPDDDEFLGARRCASLEDGLRGVDVIMALRIQKERMDTSQIPDVDGFLREWGITAST
                     IRHAKPDVVVLHPGPMNRGVEITSEIADGPQSAVQQQVRNGVAVRMAVLATIVRNVQV
                     RNVEAGRREA"
     gene            678..1652
                     /locus_tag="VD737_00830"
     CDS             678..1652
                     /locus_tag="VD737_00830"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HXW09132.1"
                     /translation="MKPDGEGVAAPAMTGAACTIDTPGRSPGKPAAERTHRGSIHVED
                     GVLLSQQEFPGRQYVIRLQAPKCAATARPGSFAHLTCDPERPMRRPLSLMRVDARAGW
                     IEILYKVVGPGLDALSKQPVGATISVLGPIGHGFVVHAERPRTLLVGGGVGIPPMVFL
                     AESLRARRSEAAWKPLVLMGSEIEFPFRARPSMILVPGVPPEAIACMPLLDDWGIASR
                     LASFGGFPGCFHGFVTDLAARWLESLDAAALAEVEMFACGPTPMLQATARVARRFGVP
                     CQVSLEEFMACAVGGCAGCAVPVVTPAGLAMKRVCVDGPVFDANSVFR"
     gene            complement(1740..>2126)
                     /locus_tag="VD737_00835"
     CDS             complement(1740..>2126)
                     /locus_tag="VD737_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HXW09133.1"
                     /translation="CLATLHANNAAETMDRIINMFPRDQHSQIFLDLSQYLKGILSQR
                     LVMGKDGRRYPACEVLLNTPHVQELIKKGDVVGVKEALVGSNEKGMRSFDVALYQLYL
                     EGKVTLEEAINNADSRSNLEAKINFG"
BASE COUNT          290 a          727 c          756 g          353 t
ORIGIN      
        1 ctacacgctg caggcgatgg gcgtcgacgt gttcgtgatg cgcgactccg agcccggcgt
       61 gccggccttc gtggccggtc acgtgcagcc ccacgcgagc gtgctgaacg caggcgaggc
      121 gcacgtctcg cacccgacgc agggcctgct cgacgtgctc acgatccgcc agcgcaaggg
      181 caaggacctg ggctcgctca gcgtcgcgat cgtcggcgac atcaggcact cgcgcgtcgc
      241 gcgctcgacc ggcacggccc tgcagacgct cggcgtcggc gacctgcgcc tggtgtcgcc
      301 gccgtcgctc gcgcccgacg acgacgagtt cctcggcgcg cggcggtgcg cgtcgctcga
      361 ggacggattg cgcggtgtcg acgtgatcat ggcgctgcgc atccagaagg agcgcatgga
      421 cacctcccag attcccgacg tcgacggttt cctgcgcgag tggggcatca ctgcgtcgac
      481 cattcgccac gcgaagcccg acgtggtcgt cctgcacccc gggccgatga accgcggcgt
      541 ggagatcacc agcgagatcg cggacggtcc gcagtcggcg gtacagcagc aggtgcgcaa
      601 cggcgtcgcc gtgcgcatgg cggtgctggc gaccatcgtg cgcaacgtgc aggtacgcaa
      661 cgtcgaggcg ggacggcgtg aagcctgacg gcgaaggcgt ggcggcacct gccatgacgg
      721 gcgcggcctg cacgatcgac acacccggtc gcagtccggg caagccggcg gccgagcgca
      781 cgcaccgcgg ctcgatccac gtcgaggacg gcgtcctgct ctcgcagcag gagttcccgg
      841 gtcgccagta cgtgatccgc ctgcaggcgc ccaagtgcgc cgcaactgcg cggcccggct
      901 cgttcgcgca cctgacctgc gaccccgagc gtccgatgcg gcggcccctg tcgctgatgc
      961 gcgtcgacgc gcgcgcaggc tggatcgaga tcctctacaa ggtcgtgggg ccgggtctcg
     1021 acgcgttgtc gaaacagccg gtcggcgcga cgatcagcgt gctcggaccg atcggccacg
     1081 gcttcgtcgt gcacgcggag cgcccgcgca ccctgctggt cggtggcggc gtcgggatcc
     1141 cgcccatggt gttcctcgcg gagtcgctgc gggcgcggcg gtccgaggcc gcgtggaagc
     1201 cgctcgtgct gatgggttcg gagatcgagt tccccttccg cgcgcgtcct tcgatgatcc
     1261 tggtgccggg agtgccgccc gaggcgatcg cctgcatgcc gctgctcgac gactggggca
     1321 tcgcgagccg gctcgcgagc tttggcggct ttccgggctg tttccacggc ttcgtcaccg
     1381 acctggcggc gcgctggctc gagtcgctgg acgccgccgc actcgccgaa gtcgagatgt
     1441 tcgcctgcgg gccgacaccg atgctgcagg cgaccgcgcg tgtcgcccgt cgcttcgggg
     1501 tgccgtgcca ggtgtcgctc gaggagttca tggcctgcgc ggtcggcggc tgcgccggct
     1561 gcgccgtgcc ggtcgtcacc ccggcgggcc tcgcgatgaa gcgcgtctgc gtcgacggcc
     1621 ccgtcttcga cgcaaactcc gtgttccgat aggtgccggt gttacggttc cggttcgtga
     1681 ccgggaccgt gatcgtcaaa ggatgaatgg cggaaccgga accgtaacac cggcaccgat
     1741 cagccgaagt tgatcttggc ctcgaggttg ctgcgcgagt cggcgttgtt gatcgcctcc
     1801 tcgagcgtga ccttgccttc caggtagagc tggtagagcg cgacgtcgaa cgagcgcatg
     1861 cccttctcgt tggagccgac cagcgcttcc ttgacgccga cgacgtcgcc cttcttgatc
     1921 agctcctgca cgtgcggcgt gttgagcagc acctcgcagg ccgggtagcg gcgcccgtcc
     1981 ttacccatca ccagccgctg cgacaggatc cccttcaggt actgtgagag gtccaggaag
     2041 atctgcgagt gctggtcgcg ggggaacatg ttgatgatcc ggtccatcgt ctcggcggcg
     2101 ttgttcgcgt gcagcgtcgc gaggca
//
LOCUS       DASPFR010000034         3859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_622318, whole genome shotgun sequence.
ACCESSION   DASPFR010000034 DASPFR010000000
VERSION     DASPFR010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622318"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2490
                     /locus_tag="VD737_00840"
     CDS             <1..2490
                     /locus_tag="VD737_00840"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HXW09134.1"
                     /translation="DLDTEFLTGSRNEAIKPRLKLRDILAQLKQIYCGTIGAEFAHVS
                     DETERLWLQDRFQVGRLTQHFGPEQRREILWHLTMAEGLERYLATRYPAQKRFSLEGG
                     DSLIPLLEQVIQCGGGHGVEDLVFGMAHRGRLNVLVNVLGKSPEALFSEFEGKYDLKA
                     LKGSGDVKYHKGFSCDVRTPSGNVHVALAFNPSHLEVVNPVVEGSVRARQERRGDTRG
                     DKVMPVLIHGDAAFAGQGVIQETLQMSQARAFYTGGTLHLIVNNQVGFTTSDPRDTRS
                     TLYCSDVAKMIEAPILHVNGDDAEAVVFAARFAVDYRMKFHKDVVIDLVCYRRLGHNE
                     ADEPAATQPVMYANIRQRPTTRQLYAEQLVKDGVLSAADADAMVDAYRRGLDEGKPQA
                     KNVVGLVGNKYTVDWSKFHDVDWSEEVATGVKPATLKDLAAKVTTYPEGFKLHRQVQK
                     VVDDRRRMAAGQQMLDWGFAETLAYATLLDEGYEIRLTGQDSARGTFFHRHAVGHDQA
                     TGATHVPLEHLKPGQPRFRVTDSLLSEEAVLGFEYGYSITDPNCLVIWEGQFGDFANG
                     AQVIIDQFISSGETKWGRIGGLTLFLPHGYEGQGPEHSSARLERFLQLCAENNMCVCV
                     PSTPAQMFHMLRRQMVRAFRKPLIVMTPKSLLRHKLSVSALDDLTHGCFHEVIGEVDD
                     LAEKNVDRVVFCSGKVYFDLLEQRRAAEVRNVAIVRIEQLYPFPAEDYAVALSHYPNA
                     REIVWCQEEPQNQGAWYQIRHRLQEPLRGDQDLLYSGRRPAAAPATGIMQMHAQEQHG
                     LVAAALTAASHEQTARATSRLRATGTSRKKS"
     gene            2487..3794
                     /gene="odhB"
                     /locus_tag="VD737_00845"
     CDS             2487..3794
                     /gene="odhB"
                     /locus_tag="VD737_00845"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005400412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HXW09135.1"
                     /translation="MSTIEVRVPQLPESVADATLVAWRKQPGDAVTRDENLVDLETDK
                     VVLEVPAPSAGVIKELKVQNGATVTGGQVLAVIEEGATATAPAASGEVARSEGQGSGK
                     GESKPAPSPGAPPTSPATAGEAKTTQGDKLAPSVRRLVEEHKLEPSSIQGSGRDGRIT
                     KGDVLKQLADKDKPAGGTPGEGQKPSPAPRPTSSAAAGAAETGRGERRVPMTRLRARI
                     AERLVQAQSTAAMLTTFNEVDLKAVNELRARYKERFEKEHGARLGFMSFFVKACVEAL
                     KRFPVVNASVDGNDVVYHEYYDVGVAVSTERGLIVPVLRDADALSFAEIEKSVVAYAT
                     RARDGALTLEELTGGTFTITNGGVFGSLLSTPILNMPQSAILGMHKIQERPVVIDGQV
                     VVRPMMYVALTYDHRVVDGREAVTFLKHIKDVVEQPARMLIEI"
BASE COUNT          694 a         1295 c         1315 g          555 t
ORIGIN      
        1 gacctcgaca ccgagttcct gaccggcagc cgcaacgaag cgatcaagcc caggctcaag
       61 ctgcgcgaca tcctcgcgca actcaagcag atctactgcg gcacgatcgg cgccgagttc
      121 gcgcacgttt ccgacgagac cgagcggctg tggctgcagg accgcttcca ggtggggcgg
      181 ctcacgcagc acttcggccc ggagcagcgc agggagatcc tctggcacct gacgatggcg
      241 gagggactcg agcgctacct cgcgacgcgg tatcccgcgc agaagcgctt ctcgctcgag
      301 ggcggcgatt cgttgatccc gctgctcgag caggtgatcc agtgcggcgg cgggcacggc
      361 gtcgaggacc tcgtcttcgg catggcgcac cgcggccgcc tcaacgtgct ggtgaacgtg
      421 ctcggcaagt cgcccgaggc gctgttctcc gagttcgagg gcaagtacga cctgaaggcg
      481 ctcaagggct cgggcgacgt caagtaccac aagggctttt cgtgcgacgt gcgcacgccg
      541 tccggcaacg tgcacgtcgc gctcgcgttc aacccgtcgc acctcgaggt cgtgaacccg
      601 gtcgtcgagg gctccgtgcg cgcgcgccag gagcgccgtg gcgacacgcg cggcgacaag
      661 gtcatgccgg tgctgatcca cggcgacgcc gcgttcgcgg gacagggcgt gatccaggaa
      721 acgctgcaga tgtcgcaggc gcgcgcgttc tacacgggcg gcacgctgca cctgatcgtc
      781 aacaaccagg tgggcttcac gaccagcgac ccacgcgaca cgcgctcgac gctttactgc
      841 agcgacgttg ccaagatgat cgaggcaccg atcctgcacg tgaacggcga cgatgcggaa
      901 gccgtcgtct tcgcggctcg cttcgcggtg gactaccgca tgaagttcca caaggacgtc
      961 gtgatcgacc tggtctgcta tcgacggctc ggccacaacg aggccgacga gcccgccgcc
     1021 acccagccgg tcatgtacgc caacatccgc cagcgtccga ccacgcgcca gctgtacgcg
     1081 gagcagctcg tgaaggacgg cgtgctgagc gcggcggacg ccgacgcgat ggtcgacgcc
     1141 taccgacgtg gcctcgacga aggcaagccg caggcgaaga acgtggtcgg cctcgtcggc
     1201 aacaagtaca ccgtggactg gagcaagttc cacgacgtgg actggtcgga agaggtcgcc
     1261 acgggcgtca agcccgcgac gctcaaggac ctcgcggcca aagtgacgac gtatccggaa
     1321 ggtttcaagc tgcaccggca ggtgcagaag gtcgtcgacg accggcgccg gatggcggcc
     1381 ggacagcaga tgctcgactg gggcttcgcc gagacgctcg cctacgcgac cctgctcgac
     1441 gaaggctacg agatccgcct gaccgggcag gacagcgctc gcggcacgtt cttccatcgt
     1501 catgcggtcg gccacgacca ggccacggga gccacgcacg tgccgctcga gcacctgaag
     1561 ccgggacagc cgcgcttccg cgtgaccgac tcgctgctgt ccgaagaggc cgtgctcggc
     1621 ttcgagtacg ggtactcgat caccgatccg aactgcctcg tgatctggga aggccagttc
     1681 ggcgacttcg cgaacggcgc gcaggtcatc atcgaccagt tcatctcgtc cggagagacc
     1741 aagtggggtc gcatcggcgg actcacgctg ttcctgccgc acggctacga ggggcagggg
     1801 cccgaacatt cgtcggcacg cctcgagcgg ttcctgcagc tctgcgccga gaacaacatg
     1861 tgcgtctgcg tgccgtcgac gccggcccag atgttccaca tgctgcgacg gcagatggtg
     1921 cgcgcgttcc gcaagccgct gatcgtgatg accccgaaga gcctgctgcg gcacaagctg
     1981 tcggtgtcgg cgctcgatga cctcacgcac gggtgcttcc acgaggtgat cggtgaggtc
     2041 gacgacctgg ccgagaagaa cgtcgaccgc gtcgtgttct gctcgggcaa ggtctatttc
     2101 gacctgctcg agcagcggcg cgcggccgag gtgcgcaacg tcgccatcgt gcggatcgag
     2161 cagctctatc cgttcccggc cgaagactac gcggtcgcgc tgtcgcacta cccgaacgcg
     2221 cgcgagatcg tctggtgcca ggaagagccg cagaaccagg gcgcctggta ccagatccgt
     2281 caccggctgc aggagccgtt gcgcggcgac caggacctgc tctattcggg aaggcggccc
     2341 gccgcggcac cggcgaccgg gatcatgcag atgcacgccc aggagcagca cggcctggtc
     2401 gcggcggcgc tgaccgccgc gtcacacgaa caaacggcgc gcgccacgag ccggctgcgc
     2461 gccaccggca ccagcaggaa gaagtcatga gcacgatcga agtccgggta ccccaactgc
     2521 ccgaatccgt cgccgacgcg acgctcgtcg cgtggcgcaa gcagcccggc gatgccgtga
     2581 cgcgcgacga gaacctggtc gacctcgaga cggacaaggt cgtgctcgag gtgccggcgc
     2641 cgtcggccgg cgtgatcaag gagttgaagg tccagaacgg cgccaccgtg accggcggcc
     2701 aggtgctcgc ggtcatcgag gaaggcgcca cggccaccgc tcccgctgcg tcgggagagg
     2761 tcgcgcgcag cgagggtcag ggctccggaa agggcgaatc gaaaccagcg ccctcacccg
     2821 gcgcgccgcc gacctcaccg gcgaccgcgg gcgaggcaaa gacgacgcag ggcgacaagc
     2881 tcgcgccgtc cgtgcgtcgc ctggtcgagg agcacaagct cgagccctcc agcatccagg
     2941 gctcgggccg cgacggtcgc atcaccaagg gcgacgtgct caagcaactg gcggacaagg
     3001 acaagccggc cggcggaaca ccgggtgagg gtcagaagcc gtcacccgcc ccgcggccta
     3061 cgtcttccgc agctgcggga gcagcggaga ccggacgcgg cgagcgccgc gtgccgatga
     3121 cgcggctgcg cgcgcggatc gccgaacgcc tggtgcaggc gcagtcgacg gccgcgatgc
     3181 tgacgacctt caacgaagtg gacctcaagg cggtgaacga actgcgggcg cgctacaagg
     3241 agcgcttcga gaaggagcac ggcgcgcgcc tcggcttcat gtcgttcttc gtcaaggcct
     3301 gcgtcgaggc gctcaagcgc ttcccggtcg tcaacgcctc ggtcgacggc aacgacgtcg
     3361 tctaccacga gtactacgac gtgggtgtcg cggtgtcgac cgagcgcggg ctgatcgtgc
     3421 ccgtgctgcg cgacgccgac gcgctctcgt tcgccgagat cgagaagtcc gtcgtcgcgt
     3481 acgcgacgcg cgcgcgtgac ggcgcgttga cgctcgagga gctgacgggt ggcaccttca
     3541 cgatcaccaa cggcggcgtg ttcgggtcac tgctgtcgac gccgatcctg aacatgccgc
     3601 agagcgcgat cctcggcatg cacaagatcc aggagcgacc cgtcgtgatc gacggccagg
     3661 tcgtcgtgcg gccgatgatg tacgtggccc ttacctacga tcaccgcgtc gtcgacgggc
     3721 gcgaagccgt gaccttcctc aagcacatca aggacgtggt tgagcagccg gcgcggatgc
     3781 tgatcgagat ctagagtttt tcgatcatgc ctttcctggc caacgtccgc accgtgctcg
     3841 aaatgatcaa gttcgagca
//
LOCUS       DASPFR010000035         4818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_12834, whole genome shotgun sequence.
ACCESSION   DASPFR010000035 DASPFR010000000
VERSION     DASPFR010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12834"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VD737_00850"
     CDS             complement(<1..416)
                     /locus_tag="VD737_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HXW09136.1"
                     /translation="MNTATHYNDKVVRQFAVMTVVWGIVGMLVGVLIAAQLLWPDLLP
                     AQSFLSYGRLRPLHTNAVIFAFGGSALFATSYYIVQRTCHVRLFGDGLAAFTFWGWQL
                     VIVLAAITLPLGITTSKEYAELEWPIDLLITVVWVA"
     gene            635..1585
                     /locus_tag="VD737_00855"
     CDS             635..1585
                     /locus_tag="VD737_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXW09137.1"
                     /translation="MQRLDRILAVIDPTVDAQPGVDRAARLARGAGAALELFECDFAP
                     ALTGAPFFDTDALRRQRDDYLAGRRGRLEQYAAGLRRDGLQVTTEVRWENPLHVGILR
                     RIKECAPDLVVKDTHYHSPLRRTFFSNTDWQLVRACPVPLLLAKPADWPQEPRIVAAV
                     DPSHRHAKPAALDGDILDAALRVAKCVDGQVEAVHAFFPAALLAATAGVAGVPLAADV
                     ATTELLEAERTRIERDVVDLAASRGLGRDAARLLQGAAAEVLPRYLDQVRASLLVMGA
                     ISRSRLQEFFIGSTAERVLDRLPCDVLVVKPPDFAEGLPF"
     gene            1674..3767
                     /locus_tag="VD737_00860"
     CDS             1674..3767
                     /locus_tag="VD737_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXW09138.1"
                     /translation="MPSANVLLISAHPADKARVADALHAIRGQPYRLEVTSTLAEGLL
                     RIKHGRVDAILLDLNLPDSAGLTTFLRAQPKATHVPIIVLVGEDADESGTEAVQRGAL
                     DFLVREQLSAAMIERVLRYATERTHTLLALKASEQRYRELFQNVTAGVFQTTADGKFM
                     AANPALIRMLGYSTEDELLELDISRDVYMDADHRQNWVRAMEEDGEVRNAELVLKRRD
                     GSKIVVLENSRAVRDADGNVLFYEGTLTDITAAHELSQQLSYDASHDALTGLTNRREY
                     ELKLQRALEMSQATGAGHAVLFLDLDQFKTVNDTCGHVAGDELLRQLGEVLQNRIRSA
                     DVIARLGGDEFAVLLHNCALDDALQVAQALLKAVRGFEFVWGASKFTLGVSIGIVAVD
                     ARFKRIAQVLNAADSACYQAKDAGRNRVAVYEDGAITSQHRHGEMGWVARAKRALSEN
                     RLFLEGQLIQPLAPGPGGEARLPHYEVFVRMRDESGRVVPPGAFLPAVERYNLSVRYD
                     RWVIEAALEWARDHREAFDRVSRLFINLTRDSVVDPETGDFIRQTLAATGVDPANVGF
                     ETAESVAIGNLGKANQLIGTLRRLGCAFALDDFGSGVSSFAYVKALGADYLKIDGMYV
                     GNLSQDRVDYAMVRSIKDIGHVMGKQVIAKSVESDAVMQKLQEIGVDYAQGFAVAPPK
                     PLSQISTVTVADLLA"
     gene            3890..>4818
                     /locus_tag="VD737_00865"
     CDS             3890..>4818
                     /locus_tag="VD737_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HXW09139.1"
                     /translation="MSTPAAKGIALAALVLSACSNDRPQQVLGTVERDRLELIAESNE
                     RIVELPVREGEHVAAGALLVRQEAGTAQPRIDQAKAAQAEAERRLADLEAGARPREIE
                     EARATLAGAEGALRTDASEYERVSALVERKLLSASELDRARARLDESRAARDAARARL
                     ELLRQGTRTEQVAEARAAVARAEAALTEVQTVADRYAVHAPRAGIVEALPYELGERPA
                     AGRPVAVLLADGAPYARVYVPEPLRSRFVAGTPVELTVDGEERRYRGVVRYVSAEAAF
                     TPYYALTQKDRSRLAYVAEIDLTDAEAARLPTGL"
BASE COUNT          770 a         1692 c         1681 g          675 t
ORIGIN      
        1 taggccaccc acaccaccgt gatcagcagg tcgatcggcc actcgagctc ggcgtattcc
       61 ttcgaggtcg tgatcccgag cggcaacgtg atcgcggcca gcacgatgac gagctgccag
      121 ccccagaacg tgaacgccgc gagcccgtcg ccgaacagcc ggacgtggca ggtgcgttgc
      181 acgatgtagt acgacgtcgc gaacagcgcc gagccgccga acgcgaagat caccgcgttg
      241 gtgtgcagcg gccgcaggcg cccgtacgac aggaaggact gcgcgggcag gaggtcgggc
      301 cagagcagct gggccgcgat gaggacgccg accagcatgc cgacgatgcc ccacacgacg
      361 gtcatgaccg cgaactgccg caccaccttg tcgttgtagt gagtggccgt attcatcgag
      421 cgcctcgagg aacgagaatg ggaataggtg ctgttggccg cgcagcacgc ggcagcgacg
      481 cgcgaatcgt aaagaccgcg cttgtcgccg cgctgtacgc acaggcagac gatcgctagg
      541 tatttgccga agcgagttgt ccggattgcg ccgcctcggg ccagcaagca catttcgtcc
      601 tgcaggcgac gcgcgtcgcg ccccggagcg actcatgcag cggctcgacc ggatcctcgc
      661 cgtgatcgat cccacggtgg acgcacaacc cggcgtggac cgggcggccc gactggcccg
      721 cggcgccggc gccgcgctgg aactcttcga gtgcgatttc gcgcccgcac tgaccggcgc
      781 gccgttcttc gacaccgatg cgctgcggcg ccagcgcgac gactacctgg ccgggcgccg
      841 tggccggctg gagcagtacg cggccggatt gcgccgcgac ggactccagg tcacgacgga
      901 ggttcgctgg gaaaacccgc tgcacgtggg catcctgcgg cgaatcaaag agtgcgcgcc
      961 tgacctggtc gtgaaggaca cgcactacca ctcaccgctg cgccggacgt tcttttccaa
     1021 caccgactgg cagctggtgc gtgcctgccc agtcccgctc ctgctggcaa agcccgccga
     1081 ctggccgcag gagccgcgga tcgtcgccgc ggtcgatccg tcgcaccgcc acgcaaagcc
     1141 cgctgcgctg gacggtgaca tcctcgacgc cgcgctgcgc gtcgcgaaat gcgtggacgg
     1201 gcaggtcgag gccgtgcacg cgttcttccc cgccgccctg ctcgccgcca cggcgggcgt
     1261 cgccggcgtt ccgctcgcgg ccgacgtcgc cacgaccgag ctgctcgagg ccgagcgcac
     1321 caggatcgag cgcgacgtcg tcgatctcgc ggcctcgcgc ggcctcggcc gtgacgcggc
     1381 gcggctgctg cagggcgcgg ccgccgaggt gctgccgcga tacctggacc aggtccgcgc
     1441 gtccctgctg gtgatgggcg ccatttcgcg gagccggctc caggagttct tcatcggcag
     1501 cacggcggag cgcgtcctcg accggctgcc ctgcgacgtg ctggtggtga agccgccgga
     1561 tttcgcggaa ggcctgcctt tttgaagtgc gtcaacaaag gccgggcagg gcgcggcggg
     1621 cccggtatgg cgtcacataa ttgggtgaac gtgcagccgt atactcgatc gcgatgccga
     1681 gcgccaacgt cctgctgatc agcgcccacc cggccgacaa ggcgcgggtg gcggacgcgt
     1741 tgcacgccat ccggggccag ccgtatcggc tcgaggtgac gtccacgctc gcggagggcc
     1801 tgctcaggat caagcacggg cgcgtcgacg cgatcctgct ggacctcaac ctgcccgaca
     1861 gcgcgggcct cacgaccttc ctgcgcgcgc agccgaaggc gacccacgtg ccgatcatcg
     1921 tgctggtggg cgaggacgcc gacgagtccg ggaccgaagc cgtgcagcgc ggcgccctcg
     1981 acttcctcgt gcgcgagcag ttgagcgcgg ccatgatcga acgcgtgctg cgctacgcga
     2041 ccgagcgcac gcacacgctg ctcgcgctca aggcctccga gcagcgctac cgcgagctgt
     2101 tccagaacgt gacggccggc gtgttccaga cgaccgcgga cggcaagttc atggcggcca
     2161 acccggcgct gatccggatg ctcggctact ccaccgagga cgagctgctc gagctcgaca
     2221 tctcgcgcga cgtgtacatg gacgcggacc atcggcagaa ctgggtgcgc gcaatggagg
     2281 aggacggcga ggtccgcaac gccgaactgg tgctcaagcg gcgcgacggc tcgaagatcg
     2341 tcgtgctcga gaactcgcgc gcggtccgcg acgccgacgg caacgtgctc ttctacgagg
     2401 gcacgctcac ggacatcacc gcggcgcacg aactctcgca gcagctctcg tacgatgcga
     2461 gtcacgacgc gctcaccggc ctcaccaacc ggcgcgagta cgagctcaag ctgcagcgcg
     2521 cgctcgagat gagccaggcg accggcgccg ggcacgccgt gctgttcctc gacctcgacc
     2581 agttcaagac ggtcaacgac acgtgcggcc acgtcgcggg cgacgagctg ctgcgccagc
     2641 tcggtgaagt gctgcagaac cgcatacggt ccgccgacgt catcgcgcgg ctcggcggcg
     2701 acgagttcgc ggtgctgctg cacaactgcg cgctcgacga cgcgctgcag gtggcccagg
     2761 cgctgctcaa ggcggtgcgc gggttcgagt tcgtgtgggg tgcgagcaag ttcacgctcg
     2821 gcgtcagcat cggcatcgtc gcggtcgacg cgcgcttcaa gcgcatcgcc caggtcctca
     2881 atgccgccga ctcggcctgc taccaggcga aggacgccgg gcgcaaccgc gtcgccgtct
     2941 acgaagacgg cgcgatcacc tcgcagcacc ggcacggcga gatgggctgg gtcgctcgcg
     3001 ccaagcgtgc gctgtccgag aaccgcctgt tcctcgaggg ccagttgatc cagccgctcg
     3061 cgcccgggcc tggcggcgag gcccggctgc cgcattacga ggtcttcgtg cgcatgcgcg
     3121 acgaatcggg ccgcgtggta ccgcccggag ccttcctgcc ggccgtcgaa cgctacaacc
     3181 tgtcggtgcg ctacgaccgc tgggtgatcg aagccgcgct cgagtgggcg cgcgaccacc
     3241 gcgaggcctt cgacagggtg tcgcggctgt tcatcaacct gacgcgcgac agcgtcgtcg
     3301 acccggaaac gggcgacttc atccgccaga cgctcgccgc gaccggcgtc gacccggcga
     3361 acgtcggttt cgagacggcc gagtcggttg ccatcggcaa cctcggcaag gccaaccagc
     3421 tgatcgggac gctgcgtcgc ctcggctgtg cgttcgcgct cgacgatttc ggcagcggcg
     3481 tgtcgtcgtt tgcgtacgtg aaggcgctcg gcgccgatta cctgaagatc gacgggatgt
     3541 acgtgggcaa cctgtcgcag gaccgtgtcg actacgcgat ggtccgctcg atcaaggaca
     3601 tcggccacgt gatgggcaag caggtcattg cgaagtccgt cgagagcgac gcggtgatgc
     3661 agaagctgca ggaaatcggc gtggattacg cgcagggttt cgcagtcgcg ccgccgaaac
     3721 cgctatcgca gatcagcacg gtgaccgtgg ccgacctgct ggcgtgaccg gacggcggcc
     3781 ctgactggga tgtcgagggg cgcccccggg cgcctttttc attgccgaag cccgaacccg
     3841 cgtcgaataa tgcagcctcg accacttccc gggccgtgac cccatcccct tgagtacacc
     3901 cgccgccaag ggcatcgcac tcgccgcgct ggtgctctcg gcctgttcga acgatcgccc
     3961 gcagcaggtc ctgggcacgg tcgaacgcga ccggctcgag ctgatcgcgg aatcgaacga
     4021 gcgcatcgtc gagttgccgg ttcgcgaagg cgagcacgtc gcggcgggcg cgctgctggt
     4081 ccggcaggaa gcaggcaccg cccagccgcg catcgaccag gccaaggccg cgcaggccga
     4141 ggccgagcgc cgcctcgcgg acctcgaagc cggtgcgcgg ccgcgcgaga tcgaggaggc
     4201 gcgtgccacg ctggccgggg ccgagggcgc cctgcgcacc gatgcgagcg aatacgagcg
     4261 cgtcagcgcg ctggtcgagc gcaagctgct cagcgcgtcg gaactcgatc gcgcccgggc
     4321 gcgactggac gaatcacgcg cggcgcgcga cgccgcgcgt gccaggctcg agctgctgcg
     4381 acagggcacg cgcaccgaac aggtcgccga ggcccgtgcc gccgtggcac gcgccgaggc
     4441 ggcgctcacg gaagtccaga ccgtcgccga ccgctacgcg gtgcacgcgc cgcgcgccgg
     4501 catcgtcgag gcgctgccct acgaactcgg cgagcgaccc gccgccggtc gcccggtcgc
     4561 ggtgctgctt gccgacggcg cgccgtacgc ccgcgtgtac gtgcccgagc cgctgcgcag
     4621 ccgcttcgtc gcgggcacgc cggtcgaact cacggtcgac ggcgaagagc gacgctaccg
     4681 cggcgtggtg cgctacgtct cggccgaggc cgcgttcacg ccgtactacg cgctgaccca
     4741 gaaggaccgc tcgcggctcg cctacgtcgc cgaaatcgac ctgacggatg cggaggcggc
     4801 gcggcttccc accgggct
//
LOCUS       DASPFR010000036         4649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1599754, whole genome shotgun sequence.
ACCESSION   DASPFR010000036 DASPFR010000000
VERSION     DASPFR010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599754"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /gene="zapE"
                     /locus_tag="VD737_00870"
     CDS             complement(<1..1079)
                     /gene="zapE"
                     /locus_tag="VD737_00870"
                     /inference="COORDINATES: protein motif:HMM:NF015902.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HXW09140.1"
                     /translation="MAPHADLRRAYEAALARHGYTADGAQLHAIARLDDLSQRLRAAD
                     SPRGGTMSRLLGRLRKASPVHGERGVYLWGGVGRGKTFLMDLFQAHVAVPSRREHFHR
                     FMKDVHGRLRELRDASDPLDLVAVAMAREIRLLCLDELFVTDIADAMLLAGLFAGLVE
                     GGVTLVFTSNVPPAELYRDGLQRQRFLPAIALIERHTEVIAVDAGYDYRLRQLEKAPL
                     YLDAAVEGARERLARRFAELGDEAPGGPGRITVEGRSIDYLAATDEAIWFEFRAICDG
                     PRSQADYVEIARDYHTVAVSDVPRLDATQENQARRFIALVDEFYDRGVKLLLSAHAPP
                     ADLYSGERLRFEFARTQSRLAEMQT"
     gene            complement(1085..3034)
                     /locus_tag="VD737_00875"
     CDS             complement(1085..3034)
                     /locus_tag="VD737_00875"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HXW09141.1"
                     /translation="MNDTTPIWRPSAEGIARANLTRFHSFAGQYGAPPGDYATLWQWS
                     VDDPAAFWSALFAFTGVIADRGAAPALQGVERMPGARWFEGTRLNFAENLLRVSGTDP
                     AVVAWDERGPRKSLSWDELHREVARVADGLREAGVGPGDRVAGFLPNVPAAVVAMLAT
                     ASLGATWSSCSPDFGIRGVLDRFGQIEPKVLFTADGYLYAGKTIDSLGRIVSTLEDLP
                     VVRRVVVVPHLVEHPDLSQVRDAVHYADFGRTGAPLVYSRQRFDHPLYILYSSGTTGT
                     PKCIVHGAGGTLLQHLKEHQLHCDLRPGDRLFFFTTCGWMMWNWLASALASGATIVLY
                     DGSPLHPDEGALWRLAETESVALFGTSPKYLAALEKSGFRPRERANLPQLRMLLSTGS
                     PLAHEQFDFVQDAIGPHVQLASISGGTDIVSCFALGNPVLPVYRGELQCRGLGMRVEV
                     FDEEGRPLRGERGELVCTRPFPSMPVGFWNDPDGRKYRAAYFERFPGTWCHGDYAVLT
                     EHDGLVILGRSDAVLNPGGVRIGTAEIYRQVEKLEDVLESLAIGQEWDNDVRVVLFVR
                     LRPGVELDEVLERRIRDVIRTNTTPRHVPARIVAVPDIPRTISGKIVELAVREVVHGR
                     PVRNTDALANPQALEHFRDLPALRS"
     gene            complement(3031..>4649)
                     /locus_tag="VD737_00880"
     CDS             complement(3031..>4649)
                     /locus_tag="VD737_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HXW09142.1"
                     /translation="IMVTDLAFDAFRFAQYADTVPAIAHERAHAPIGGWLSTLVGRWS
                     PAALAVSAAIAYWTQMVTVCAFLVLLPLGEHFHIVTALPALYFRRGRPANQVPAVDLS
                     PLLDDSADASELKVGARTVADLSWKDGLDAFTCTECGRCKDACPAFLTGKPLSMKWVN
                     DGLKHHLLEQRATLVKGDSAAREQLPPLVGNVIAEDTLWSCTTCGYCEAACPIELELL
                     PKFYRLRQHRVMMDGEFPAELKNVFDAYEVQSNPWGLPADTRGDWARALDVPVIANAE
                     QMAQVDYLFYVGSAESFDVRGQKIARAFVQVLREAGVSFGILGPREPTTGECVRRVGN
                     EMLFQQLAATLVETFTELGVRRIVTCDPHAYNSLRNEYPAFGGQWEVVHHTQLMARLL
                     DEGRIAVRPTFARVMLHDPCYLGRHNGEYEAPRSVLRRLTLDAPLEFPLNRQKAMCCG
                     AGGGRMWMEEHIGTRINVARVEQALPQNPGVIATACPYCAVMVGDGLKAKDLHEQTGA
                     QDVAELVAAALVRRTDGRLVPTPARDKVEA"
BASE COUNT          725 a         1587 c         1642 g          695 t
ORIGIN      
        1 ttggtctgca tttcggccag gcggctctgc gtccgggcga attcgaagcg cagccgctcg
       61 cccgaataga gatcggcagg tggggcgtgc gcggacagca gcaacttgac gccgcggtcg
      121 tagaactcgt cgacgagcgc gatgaagcgc cgggcctggt tctcctgcgt ggcgtccagg
      181 cgcggcacgt cggacacggc gacggtgtgg tagtcgcggg cgatctcgac gtagtcggcc
      241 tgggagcgag gcccgtcgca gattgcgcga aactcgaacc agatcgcttc gtcggtcgcc
      301 gcgaggtagt cgatgctgcg accttcgacc gtgatccgcc cgggcccgcc cggcgcctcg
      361 tcaccgagtt cggcgaaccg ccgtgccagc cgctcccgtg cgccttcgac ggctgcatcg
      421 aggtacaggg gcgctttctc gagctgtcgc agccggtagt cgtagcccgc gtcgacggcg
      481 atcacctcgg tgtgtcgctc gatgagcgcg atggccggca ggaagcgttg ccgctgcagc
      541 ccgtcgcggt acagctcggc cggcggcacg ttggacgtga acaccagcgt gacgccgccc
      601 tcgacgaggc ctgcgaagag cccggcgagc agcatggcgt ccgcgatgtc cgtgacgaag
      661 agctcgtcga ggcacaacag ccggatctcg cgcgccatcg cgacggcgac gagatcgagt
      721 ggatcgctcg cgtcgcgcag ctcgcgcagc cgcccgtgca cgtccttcat gaagcggtgg
      781 aagtgctcgc gccgcgacgg caccgcgacg tgcgcctgga acaggtccat caggaacgtc
      841 ttgccgcgcc cgacgccgcc ccacaggtac acgccccgct ccccgtggac gggcgacgcc
      901 ttgcgcagtc gtccgagcag gcgggacatc gtgcctcccc gcggtgaatc tgccgcgcgc
      961 agccgctgcg aaagatcgtc gagccgggcg atcgcgtgca gctgcgcgcc gtcggcggtg
     1021 tagccgtggc gggcgagcgc cgcttcgtac gcccggcgca ggtctgcgtg cggggccatc
     1081 cgggtcagct gcgcagggcc ggcaggtcgc gaaagtgttc cagtgcctgc ggattcgcga
     1141 gggcatcggt gttgcgcacc ggacgaccgt gcacgacttc gcgcacggcg agctcgacga
     1201 tcttgccgct gatcgtgcgc gggatgtccg gcacggccac gatgcgcgcg ggcacgtggc
     1261 gcggcgtcgt gttggtgcgg atcacgtcgc ggatgcgacg ctcgagcacc tcgtccagct
     1321 cgacgcctgg gcgcagccgc acgaacagca cgacccgcac gtcgttgtcc cattcctggc
     1381 cgatcgcgag gctctcgagg acgtcctcga gtttctcgac ctgccggtag atctccgccg
     1441 tgccgatgcg gacaccgccc gggttcagca ccgcgtccga ccggcccagg atcacgaggc
     1501 cgtcgtgctc ggtgagcacg gcgtagtcgc cgtggcacca ggtgcctgga aagcgctcga
     1561 aatacgccgc gcgatacttg cgtccgtcgg ggtcgttcca gaacccgacg ggcatcgacg
     1621 ggaacggccg cgtgcagacg agctcgccgc gctcgccacg cagcggtcgg ccctcttcgt
     1681 cgaagacctc gacccgcatg ccgaggccgc ggcactgcag ctcgcctcga tacacgggca
     1741 gcacgggatt gccgagcgcg aagcacgaca cgatgtcggt gccgcccgat atcgaggcga
     1801 gctggacgtg cggcccgatc gcgtcctgca cgaagtcgaa ctgctcgtgc gcgagcgggg
     1861 agccggtcga gagcagcatg cgcagctgcg gcaggtttgc gcgctcgcgc ggccggaatc
     1921 cgctcttctc cagcgccgcg aggtacttgg gactggtgcc gaacagcgcg acggattccg
     1981 tctcggccag gcgccagagc gcgccttcgt cgggatgcag tggcgagccg tcgtagagca
     2041 cgatcgtggc cccgctcgcg agtgcgctcg cgagccagtt ccacatcatc cagccgcagg
     2101 tggtgaagaa gaacagccga tcgccgggtc gaaggtcgca gtgcagctgg tgctccttca
     2161 ggtgctgcag cagcgtgccg cccgccccgt ggacgatgca cttgggcgtg ccggtcgtgc
     2221 ccgacgagta gaggatgtag agcgggtgat cgaaccgctg gcgcgagtac acgagtggag
     2281 cgcccgtgcg tccgaagtct gcgtaatgaa ccgcgtcacg cacctgcgac aggtcaggat
     2341 gctcgacgag atgcggcacg acgacgacgc gacgtaccac cggcaggtcc tcgagggtgg
     2401 agacgatccg cccgagcgag tcgatcgtct tgccggcgta gagatagcca tcggcggtga
     2461 acagcacctt gggttcgatc tgcccgaatc gatccagcac gccccgtatg ccgaaatccg
     2521 gcgagcacga cgaccaggtc gcgccgaggc tggctgtggc gagcatggcc acgacggcgg
     2581 ccggaacgtt cgggaggaag ccggccacgc gatcgccggg cccgacgccg gcctcacgta
     2641 ggccgtctgc cacgcgcgcg acttcgcgat gcagctcgtc ccacgacaac gacttccgcg
     2701 ggccgcgctc gtcccaggcg acgacggcgg gatcggtccc cgagacacgc agcaggttct
     2761 cggcgaaatt gaggcgggtg ccttcgaacc acctggcgcc cggcatgcgt tcgacgccct
     2821 gcagcgccgg ggccgctccg cggtccgcga tcacgcccgt gaaagcaaag agcgcactcc
     2881 agaacgcggc tgggtcgtcc accgaccatt gccacagcgt cgcgtaatcg ccgggaggcg
     2941 cgccgtattg cccggcgaac gagtgaaatc gagtgaggtt ggcgcgggcg ataccctcgg
     3001 ccgagggccg ccagatcggg gtcgtgtcgt tcacgcctcc actttatcgc gcgcaggcgt
     3061 gggcaccaac cgcccgtcgg tgcgtcgcac cagcgcggcg gcgacgagtt ccgcgacgtc
     3121 ctgcgccccg gtctgctcgt gcaggtcctt cgccttgagg ccgtcgccga ccatgaccgc
     3181 gcagtacggg cacgccgtgg cgatgacgcc ggggttctgc ggcagggcct gttcgacgcg
     3241 cgccacgttg atgcgcgtgc cgatgtgttc ctccatccac atgcggccgc cgccagcgcc
     3301 gcagcacatg gctttctgcc ggttcagcgg gaattcgagc ggcgcgtcga gggtcaggcg
     3361 ccgcaggacg ctgcggggtg cctcgtactc gccgttgtgg cgcccgaggt agcaggggtc
     3421 gtgcagcatc acgcgcgcga acgtgggtcg gacggcgata cggccttcgt cgagcagccg
     3481 ggccatcaac tgcgtgtggt gcacgacctc ccactggccg ccgaaggccg ggtattcgtt
     3541 gcgcagcgag ttgtaggcgt gcgggtcgca ggtgacgatg cggcgcacgc ccagttccgt
     3601 gaacgtctcg accagcgtcg cggccagttg ctggaacagc atctcgttgc cgacgcgccg
     3661 cacgcactcg ccggtggttg gttctcgcgg tcccaggatg ccgaagctga cgcccgcttc
     3721 gcgcagcacc tgcacgaacg cacgcgcgat cttctgccct cgcacgtcga aagactcggc
     3781 agagccgacg tagaacaggt agtcgacctg cgccatctgc tccgcgttcg cgatgaccgg
     3841 cacgtcgagt gcgcgcgccc agtcgccgcg cgtgtcggcg ggcaagcccc aggggttcga
     3901 ctgcacctcg taggcgtcga acacgttctt gagttcagcc gggaactcgc cgtccatcat
     3961 cacgcggtgc tggcgcagcc ggtagaactt cggcagcagt tccagctcga tcgggcaggc
     4021 ggcctcgcag tagccgcacg tggtgcacga ccagagcgtg tcctcggcga tcacgttgcc
     4081 gacgagcggc ggcagctgct cgcgcgccgc gctgtcgccc ttcaccagcg tggcgcgctg
     4141 ctccagcagg tggtgcttca agccgtcatt gacccacttc atggacagcg gcttgcccgt
     4201 caggaaagcc gggcacgcat ccttgcagcg accgcactcg gtgcaggtga aggcgtcgag
     4261 cccgtccttc cacgagaggt ccgcgaccgt gcgcgcgcca accttgagtt cgctggcgtc
     4321 ggcggaatcg tccagcaacg ggctcaggtc cacggcgggc acctggttcg cgggccggcc
     4381 gcgacggaag tagagcgccg gcaacgcagt gacgatgtgg aaatgctcgc cgagcggcag
     4441 cagcacgagg aacgcgcaga ccgtcaccat ctgcgtccag tacgcgatcg cggcggacac
     4501 cgcgagcgcc gcgggcgacc agcgaccgac cagcgtcgag agccagccgc cgatcggcgc
     4561 gtgcgcgcgc tcgtgcgcga tggccggcac ggtgtccgcg tactgcgcga accggaacgc
     4621 gtcgaacgcg aggtcggtga ccatgatga
//
LOCUS       DASPFR010000037         3375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_13205, whole genome shotgun sequence.
ACCESSION   DASPFR010000037 DASPFR010000000
VERSION     DASPFR010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13205"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..2182)
                     /locus_tag="VD737_00885"
     CDS             complement(260..2182)
                     /locus_tag="VD737_00885"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HXW09143.1"
                     /translation="MSMKLRRGTGFVSLLLLSSFATLTSAATVPAGFDDSPFVQLPSD
                     ATAMQFAPDGRLFVCQQSGKLRVVLNGTLLSTPFLTLPVDAAGERGLLGVALDPDFER
                     NQYVYVHYTATAPTIHNRVSRFTANGNVAVPGSEVVLLNLDPLSTARNHNGGALHFGN
                     DGKLYVAVGDNANPDNAQLLGNPFGKVLRINRDGSIPADNPFVDRTSGIDRAIWALGL
                     RNPFTMAFQRSSGRLLINDVGQQTFEEINTGVAGANYGWPAAEGPSSNPAYADPLHYY
                     GHADGSCAVTGGTFYEPNAVTFPGWNFGKYFFADYCGGWIRRLNPDTGAITDFATGVA
                     GPVDLKVGPDGALYYLARNAAVVGRIGYDTAQSPSINLQPASRTVAVGQSVTFKVGAT
                     GSSPLAYQWRRNGTPITGATAASYTRSNVQLLDDGAGFDVVVTNAFGRATSATARLTV
                     LQETVPTASITRPVAGTRYTAGSTIAYAGTGNDVEDGVLPASAFTWWVNLHHDGHTHP
                     LVLPVSGSKSGSFTIPATGETSANVFYRIHLRVRDSAGLTRSVRRDVLPRKSTVTLAS
                     NPTGLKLKLDGQAVTTPYRFVGVVGMQRKLEAVSPQSANGASWTFQGWSDGGARIHTI
                     RTPGTDTTYTARFTSP"
     gene            complement(2393..3301)
                     /locus_tag="VD737_00890"
     CDS             complement(2393..3301)
                     /locus_tag="VD737_00890"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXW09144.1"
                     /translation="MSDLRARLQANDFDPEVLHVFDRYVHGDIDRREFLERVGRLALG
                     TMTAAGVLGALSPRFAEAQQVGPGDERIDARYVEYDSPRGYGRGRGYLVRPAKPGRQA
                     PPVVMVVHENRGLNPHIEDVARRLALEEFVALAPDALFPLGGYPGDEDQARTLFQTLD
                     QEKCFEDFIAATQFLRAQPGVRDRLGVVGFCYGGAIANRLAVALPDLAVAVPFYGSAP
                     ALANVADIEAELLLHFAGDDERVNATWPAYEAALRAARVRYTAHVYPGTQHGFHNDTT
                     PRYDERAAKRAWKRTIALFDRRLRRS"
BASE COUNT          525 a         1128 c         1174 g          548 t
ORIGIN      
        1 gggcgtgagc cgcgcgtcgt cgtggcgcgg ggtcgattgc tcgtgagcgc gcgcggggat
       61 tgcggccaag gggcctcctg gtcgagttcg tcgccgtcgg ttcggacgaa tgtctcagaa
      121 cgcggcgcgt cgaacgcaca aggccgtcac agaatgcgtg cactccccca ccgcgcgcgg
      181 cggtttatgt caaggatcgg ggcggcgcgg accgcgacgc cggccggcgt cgccccaggg
      241 tggtcgctcg tgccgagcat caaggcgagg tgaaccgcgc ggtgtaggtg gtgtcggtgc
      301 ccggcgtgcg gatcgtgtgg atgcgcgccc cgccgtccga ccagccctgg aaggtccacg
      361 atgcgccatt ggcggactgc ggcgacactg cttcgagctt gcgctgcatg cccaccacgc
      421 ccacgaagcg gtacggcgtc gtcacggcct ggccgtcgag cttcagtttc aagcccgtcg
      481 ggttcgacgc cagcgtcacg gtgctcttgc gcggcagcac gtcgcggcgc acggacctgg
      541 tgagtccggc cgagtcgcgg acccgcaggt ggatgcggta gaagacgttc gcggacgtct
      601 ctcccgtcgc cggaatcgtg aacgatccgg acttcgaacc gctcaccggc agcaccagcg
      661 ggtgcgtgtg gccgtcgtgg tgcaggttca cccaccaggt gaacgcgctc gccggcagca
      721 cgccgtcttc gacgtcgttg ccggtgccgg cgtacgcgat cgtgctgccc gccgtgtagc
      781 gggtgcccgc cacgggcctg gtgatgctcg ccgtcggcac cgtctcctgc agcaccgtca
      841 ggcgcgcggt ggcgctcgtc gcacggccaa aggcattcgt gacgacgacg tcgaaaccgg
      901 cgccgtcgtc gagcagctgc acgttgctgc gcgtgtagct cgcagcggtg gctcccgtga
      961 tcggcgtgcc gttgcgtcgc cactggtagg ccagcggcga cgagccggtg gcgcccacct
     1021 tgaacgtgac cgactgaccg accgcgaccg tgcggctcgc cggctgcagg ttgatcgacg
     1081 gtgactgcgc cgtgtcgtaa ccgatgcgtc caacgacggc cgcattgcgc gcgaggtagt
     1141 agagtgcgcc gtccgggccc accttgaggt cgacggggcc ggcaacgccg gtggcgaagt
     1201 cggtgatggc gcccgtgtcg ggattcaggc gccggatcca gccgccgcaa tagtccgcga
     1261 agaaatactt gccgaagttc caccctggga acgtgacggc gttcggctcg tagaacgtgc
     1321 caccggtgac tgcgcacgac ccgtccgcgt gaccatagta gtgcagcggg tccgcgtagg
     1381 ctggattcga cgacggcccc tcggccgccg gccatccata gttggcaccg gcgacgcctg
     1441 tgttgatctc ctcgaacgtc tgctggccca cgtcgttgat cagcaggcgc ccgctgctgc
     1501 gctggaaggc catggtgaaa ggattgcgca gtcccagcgc ccagatggcc ctgtcgatgc
     1561 cgctggtgcg gtcgacgaac gggttgtccg cgggaatgga gccatcccgg ttgatgcgca
     1621 gcaccttgcc gaacggattg cccagcagct gcgcattgtc gggattcgcg ttgtccccca
     1681 ctgcgacgta gagcttgccg tcgttgccaa agtgcagcgc gccgccgttg tggttccggg
     1741 ccgtcgacag cgggtcgagg ttcagcagca ccacttcgct gccgggcacg gcgacgttgc
     1801 cgttcgcagt gaagcgactg acgcgattgt ggatcgtcgg ggcggtcgcc gtgtaatgca
     1861 cgtagacgta ctggttgcgc tcgaaatccg gatcgagtgc gacaccgagc agcccgcgct
     1921 cgccggccgc atcgaccggc agggtcagga acggcgtcga gagcagcgtg ccgttcagca
     1981 cgacgcgcag cttgccgctc tgctggcaca cgaacagtcg cccgtcgggc gcaaactgca
     2041 tcgcggtcgc gtccgatggc agctggacga acgggctgtc gtcgaagccc gccgggacgg
     2101 tggcagccga cgtgagcgtg gcgaacgagg acagcagcag cagcgacacg aagcccgtgc
     2161 cgcgtcgaag tttcatgctc atggagtccg tcgctggccg gcggtgaaaa tgcagaccct
     2221 gaggacggca gacttccacg tcgtgagcag cgcggccgtc ggctcacggt ccagcgtcct
     2281 gtcggcggcc ggcaccagtc ggcgaggatg gcgccagagg cgggtatgtt tacgcaggta
     2341 tcaacttgag cgagagttca taaatcatcc gtaaactact gatttaattt aattaactac
     2401 gtcgaagtcg tcgatcgaag agagcgatcg tccgcttcca ggcccgcttc gccgcccgct
     2461 cgtcgtagcg aggcgtggtg tcgttgtgga acccgtgctg cgtgcccgga tagacgtgcg
     2521 cggtgtacct cacgcgcgca gccctcagcg cggcctcgta cgccggccac gtcgcattga
     2581 cgcgctcgtc gtccccggcg aagtggagca gcaactcggc ctcgatgtcg gccacgttcg
     2641 cgagcgctgg cgcgctgccg tagaacggga cggccaccgc aaggtccggc aacgcgaccg
     2701 cgagtcgatt cgcgatcgcg ccgccatagc agaaaccgac gacgcccagg cgatcgcgaa
     2761 cgccgggctg cgcgcgcagg aactgcgtcg ccgcgatgaa atcctcgaaa cacttctcct
     2821 ggtccagggt ctggaacaac gtccgcgcct ggtcctcgtc accggggtag ccgccgagcg
     2881 ggaacaacgc atcgggcgcg agcgccacga actcctcgag cgcgaggcga cgcgcgacgt
     2941 cttcgatgtg cgggttgagc ccgcgattct cgtgcacgac catgaccacc ggcggcgcct
     3001 gcctgcctgg cttggcgggt cgcacgaggt aaccgcgccc gcgcccgtaa ccgcgcggtg
     3061 agtcgtactc cacgtagcgg gcgtcgatgc gttcgtcgcc gggccccacc tgctgcgcct
     3121 ccgcgaagcg cggactcagc gcgcccagta cgcccgccgc ggtcatcgtg ccgagcgcga
     3181 gccggccgac gcgctcgagg aactcgcgtc ggtcgatgtc gccgtgcacg taccgatcga
     3241 acacgtgcag cacctcggga tcgaaatcgt tcgcttgcag ccgcgctcgc agatcgctca
     3301 tgccctggct ccccactcgt cgttcgacgc cgtccgtgcg ccgcgagctt agcctgccgc
     3361 aggtccgcga agaag
//
LOCUS       DASPFR010000038         5117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1600263, whole genome shotgun sequence.
ACCESSION   DASPFR010000038 DASPFR010000000
VERSION     DASPFR010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600263"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VD737_00895"
     CDS             complement(<1..423)
                     /locus_tag="VD737_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXW09145.1"
                     /translation="MTRTLAFVAPREPRPMGTLLELQADRAPNAPALTCAGTTLSRAE
                     LARRAAARARALRASGVEAGDFVALALPTGPALLELAFGCWRLGATPAPLSHRLPRLE
                     LEALLAVLQPRLVVGGGGAEEPGTFLDERDLRDGPVDAE"
     gene            complement(420..2138)
                     /locus_tag="VD737_00900"
     CDS             complement(420..2138)
                     /locus_tag="VD737_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HXW09146.1"
                     /translation="MRTTPEAKIREYRDRGWWSDRRIPDLFDAAVQAAPDQVALIDPP
                     NRPALLGDAARRLTFREVADLVDGFALRLQELGLGRDDVLITQLPNVVEYPALYLAAM
                     RLGVIVSPVPMQFRRHELEQVTDLTGARAALTVRELKGGAYAATAVELAAGRALQVLC
                     LNDDTVPGAIGFTAADVTPVTRGRLREHVAGLGLEADDVATVCWTSGTEGLPKGVPRS
                     HNHWIAISYGHWRGAGIRRGERLLNPFPLINMAGLGGCFMSWMHSVGTLVLHHPLDLG
                     VYLRQIVEERPQYAIAPPAVLNMLLKDEKTLASVDLSSLRCVGSGSAPLDPEMIAGYR
                     DRFGIEIVNIFGSNEGVSLLSNAVNAPDPVHRARLFPRWGRDEVHADPPSPVQIRTRL
                     VDPETGHEVLERGRAGEMQITGPSVFDGYFRAPQVTAQSFTEDGWFRTGDLFELVGEE
                     DPAPYYRFVGRLKQIIVRGGMKIAPEELDSVLSQMPEVLEGAVSAYADPIMGERICAI
                     VVPRPGAQPSLEAVRAHFERAGLATFKYPERLLCVEQLPRNSVGKLVRGDLARMAVAP
                     PADVAP"
     gene            2225..2737
                     /locus_tag="VD737_00905"
     CDS             2225..2737
                     /locus_tag="VD737_00905"
                     /inference="COORDINATES: protein motif:HMM:NF019473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXW09147.1"
                     /translation="MDRRHFLEVSTTVTAGLFAATAGAATDPVSEPLQVLREVILAWR
                     RKDLDAMLQHIDDDIAWHSHVGSPPLQGKVAMREFATRLTAQMQEIRWRLFEAARDGD
                     RLHVEGVDDFVSNDGRRVVVPYAGVMRFRDGRIVEWRDYFDRALFERLKAGEPLPAYL
                     EGLANRPALF"
     gene            complement(2757..3515)
                     /locus_tag="VD737_00910"
     CDS             complement(2757..3515)
                     /locus_tag="VD737_00910"
                     /inference="COORDINATES: protein motif:HMM:NF006118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXW09148.1"
                     /translation="MDIKGCVALVTGGNRGIGEAFVRVLLDSGASRVYAGSRDAAGAA
                     HLERDYPGRCLGVELDVTDAAEVEAAAQRCGDVSLLVNNAGLFTNQLLLGAPDMTGAR
                     QEMEVNYFGTLAMCRAFAPILGRNGGGAIVNVLSAAAIVCVPNMGGYGPAKAATRSLS
                     GAVRAELGPQGTQVTALIVGSVDTRMAAHVEGAKEKPEDIARAGLKAVTRGIDELDTD
                     RMAVEVRAALALDPKGLEKRMARMLHAPVIRTGR"
     gene            complement(3544..>5117)
                     /locus_tag="VD737_00915"
     CDS             complement(3544..>5117)
                     /locus_tag="VD737_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXW09149.1"
                     /translation="FKHAADAVTQWLLARSLPADRSLLILSGNSVAHAVIKFGAMAAR
                     VPVCPVSANYSLMPGDFGRLRHVVSLVRPAVVFAEQTATFRRALETVDFGDAEIVTAE
                     PALLRKRATAWSDVLATAVTPAVAASIEALDPDAPSLYMLTSGSTSMPKAVIQTQRMV
                     TANVAQGRQVLAQTAGWEETMLDWLPWSHVSGACNLFATISSGGNFYIDAGRPLPGQF
                     EESLRNLREIPPSFYINVPFGYAMLVEALEADAELRERFFSRLRLALYGGAGLPQALY
                     DRFQRLAVATVGERIFFTTGYGATETASGCMSIYFPTEEVGIGLPMPGMTLKLVPRGP
                     RYEVLVRGQIVTPGYLGNAEANRAIFDEEGFYRTGDTAQFHDPDDVGKGLKFAGRLAE
                     EFKLATGTWVAAGRVRAEVLEATAPLLSDVVVCGENLGYVALLAWPRPPVAPDLRPQL
                     ATRLAAYNAGRGLSERIERLLLLSEPPSADQHEVSDKGTINQRVTLARRAGDVERLYA
                     EPPAPDVVFPFAAAH"
BASE COUNT          750 a         1793 c         1828 g          746 t
ORIGIN      
        1 ctcggcatcc accggaccgt cacgcaggtc tcgttcgtcc aggaaggtgc cgggctcttc
       61 ggcgccgccg ccgccgacga ccaggcgcgg ctgcagcacc gcgagcagtg cctcgagctc
      121 gagccggggc agccggtgtg agagcggcgc cggcgtcgcg ccgaggcgcc agcagccgaa
      181 cgcgagttcg agcagggcgg gtcccgtcgg cagcgccagc gcgacgaaat caccagcctc
      241 gacgcccgac gcgcgcagcg cacgtgcgcg tgccgcggca cgccgggcga gttcggcacg
      301 actgagcgtg gtcccggcgc aagtgagcgc cggcgcgttc ggggcgcggt ccgcctgcag
      361 ttcgagcagc gtgcccatcg gccgcggctc gcgcggcgcg acgaacgcaa gcgtgcgcgt
      421 catggagcga cgtcggccgg tggcgcgacg gccatccgcg cgagatcacc gcgcaccagc
      481 ttgccgacgc tgttgcgcgg caactgctct acgcagagca gtcgctcggg atacttgaag
      541 gtcgcgagcc cggcgcgctc gaagtgcgcg cgcacggcct cgagcgacgg ctgcgcgccc
      601 gggcggggca cgacgatcgc gcagatgcgc tcgcccatga tggggtctgc gtaggccgac
      661 acggccccct cgagcacctc gggcatctgc gacagcaccg agtcgagctc ctccggcgcg
      721 atcttcatgc cgccgcggac gatgatctgc ttgagccgtc ccacgaagcg gtaatacggt
      781 gcggggtctt cttcgccgac cagctcgaac aggtcgccgg tgcggaacca gccgtcttcc
      841 gtgaacgact gcgccgtgac ttgcggcgcg cggaagtagc cgtcgaacac cgacgggccc
      901 gtgatctgca tctcgccggc gcggccgcgc tcgagcacct cgtggcccgt ctccggatcg
      961 accaggcgtg tgcggatctg cacgggcgag ggcgggtccg cgtgcacttc gtcgcggccc
     1021 cagcgcggga acaagcgggc gcggtgcacg ggatccgggg cgttgaccgc gttgctgagc
     1081 agcgacaccc cttcgttgga cccgaagatg ttgacgatct cgatgccgaa gcggtcgcgg
     1141 tagcccgcga tcatctccgg atcgagcggc gccgagcccg acccgacgca gcggagcgaa
     1201 gagagatcga cgctggccag cgtcttctcg tccttgagca gcatgttcag caccgccggc
     1261 ggcgcgatcg cgtactgcgg tcgttcctcg acgatctggc gcaggtagac gccgaggtcg
     1321 agcggatggt gcagcaccag cgtgccgacg ctgtgcatcc agctcatgaa gcagccgccg
     1381 aggcccgcca tgttgatcag cggaaacggg ttcagcaggc gctcgccgcg ccggatgccg
     1441 gcgccgcgcc agtgcccgta gctgatcgcg atccagtggt tgtgcgagcg cggcacgcct
     1501 ttcggcaggc cttcggtccc ggacgtccag cagaccgtcg ccacgtcgtc ggcctcgaga
     1561 ccgaggcccg cgacgtgctc gcgcaggcgc ccgcgcgtca ccggcgtcac gtcggccgcg
     1621 gtgaagccga tcgcaccggg cacggtgtcg tcgttcaggc agagcacctg cagcgcacgg
     1681 ccggcggcga gttccaccgc ggtggcggca tacgcgccgc ccttgagttc gcgcacggtc
     1741 agcgcggccc gggccccagt caggtccgtg acctgctcga gctcgtggcg ccgaaactgc
     1801 atcggcacgg gactcacgat cacgccgagt cgcatcgcgg ccaggtagag cgccgggtac
     1861 tccacgacgt tcggcagctg cgtgatcaac acgtcgtcgc gaccgaggcc gagttcctgc
     1921 agccgcagcg caaacccgtc gaccaggtcc gcgacctcgc gaaacgtgag ccgacgcgcg
     1981 gcgtcgccga gcagggcggg gcggttcggg gggtcgatca gcgcgacctg gtcgggagcg
     2041 gcctgcaccg ccgcgtcgaa caggtccggg atgcgccggt cggaccacca gcctcggtcg
     2101 cgatactcgc ggatcttcgc ttcaggcgtc gtgcgcatct tcactcccgt catcggtgcc
     2161 ggctggcgtt gccggggcgc gtgttctatg gtccgtggct accgaccggc acacggagcg
     2221 tttcatggat cgtcgtcatt ttctcgaagt gtcaaccacc gtcacggccg gcctgttcgc
     2281 ggcgactgcc ggggccgcca cggacccggt cagcgagccg ctgcaggtgc tgcgcgaggt
     2341 gatcctcgcc tggcgtcgca aggacctcga cgcgatgctg cagcacatcg acgacgacat
     2401 cgcgtggcac tcgcacgtcg gcagtccgcc gctgcagggc aaggtcgcga tgcgcgagtt
     2461 cgcgacgcgc ctgacggcgc agatgcagga gatccgctgg cgcctgttcg aggcagcgcg
     2521 cgacggcgac aggctgcacg tcgagggcgt ggacgacttc gtctcgaacg acggccgccg
     2581 ggtcgtcgtc ccgtacgccg gcgtgatgcg gtttcgcgac ggtcgcatcg tcgaatggcg
     2641 cgactacttc gaccgcgcgc tgttcgagcg cctgaaggcc ggcgagccct tgccggccta
     2701 cctggagggc ctcgcgaacc gaccggcact gttctgacgg ctgcgccacg cggatgtcag
     2761 cgcccggtac ggatcaccgg cgcgtgcagc atccgcgcca tgcgtttctc gagccccttg
     2821 gggtcgagcg cgagtgcggc gcgcacctcg acggccatgc gatcggtgtc gagctcgtcg
     2881 atgccgcgtg tcaccgcctt gagaccggcg cgggcgatgt cttcaggttt ttccttggcg
     2941 ccttcgacgt gggcagccat ccgcgtatcg accgagccca cgatcagcgc ggtcacctgg
     3001 gtgccctgtg gccccagttc tgcgcgcacg gcaccggaca ggctgcgcgt ggccgccttg
     3061 gccggcccat agccgcccat gttcggcacg cagacgatcg ccgcggcgga gagcacgttg
     3121 acgatggcgc ccccgccgtt gcggcccagg atcggcgcaa aggcacgaca catcgcgagc
     3181 gtgccgaagt agttgacttc catctcctgg cgcgcgcccg tcatgtcggg ggcgccgagc
     3241 agcagctggt tggtgaacag ccccgcgttg ttgaccagca ggctcacgtc accacagcgc
     3301 tgcgccgcgg cctcgacctc ggccgcgtcg gtcacgtcga gctctacgcc caagcagcgg
     3361 cccgggtagt cgcgctcgag gtgcgcggcg cccgcggcat cgcgcgatcc ggcgtacaca
     3421 cgactcgcgc ccgagtcgag cagcacccgc acgaaggcct cgccgatgcc gcgattgccg
     3481 cccgtcacga gcgcgacgca tcccttgatg tccatgagtt cgactccggt ctgggccgtc
     3541 gcgtcagtgc gcggcggcga aaggaaagac gacgtccggc gcaggcggtt cggcgtacag
     3601 ccgctcgacg tcgccggcgc ggcgtgcgag cgtgacccgc tggttgatcg tgcctttgtc
     3661 ggacacttcg tgctggtccg cgctcggcgg ctcgctgagc agcagcagcc gctcgatgcg
     3721 ttcgctgagg ccgcggccgg cgttgtaagc cgcgagccgt gtggccagct gtggccgcag
     3781 gtccggcgcc accgggggac gtggccaggc gagcagcgcg acgtatccca ggttctcgcc
     3841 gcagacgacg acatccgaca gcagcggcgc cgtggcctcg agcacctcgg cgcgcacgcg
     3901 tccggcggcg acccaggtgc ccgtggcgag cttgaactcc tcggcgagcc gccccgcgaa
     3961 cttgaggccc ttgccgacat cgtcgggatc gtggaactgg gccgtgtcgc cggtgcggta
     4021 gaacccttcc tcgtcgaaga tggcgcggtt ggcttcggcg ttgccgagat agcccggcgt
     4081 gacgatctgc ccgcgcacga gcacttcgta gcgcgggccg cgcggcacca gcttgagggt
     4141 catgcccggc atcggcaggc cgatgccgac ttcctcggtg gggaagtaga tcgacatgca
     4201 acccgaggcg gtctcggtcg cgccgtagcc ggtcgtgaag aagatgcgct cgccgacggt
     4261 cgcgacggcg agtcgctgga agcggtcgta cagggcctgt ggcaggccgg cgccgccgta
     4321 cagcgcgagc cgcagccggc tgaagaagcg ctcgcgcagt tcggcgtcgg cctcgagcgc
     4381 ctcgaccagc atcgcgtagc cgaacggcac gttgatgtag aagctcggcg ggatctcgcg
     4441 caggttgcgc agcgactcct cgaactggcc gggcagcggc cgtcctgcgt cgatgtagaa
     4501 gttgccgccg ctcgagatcg tcgcgaacag gttgcaggcg cccgagacgt ggctccacgg
     4561 cagccagtcg agcatggtct cctcccagcc ggccgtctgc gcgagcacct gccggccctg
     4621 ggcgacgttg gccgtgacca tgcgctgcgt ctggatcacc gccttgggca tgctggtcga
     4681 gcccgaggtc agcatgtaga gcgacggcgc gtccggatcg agggcttcga tcgaggccgc
     4741 gacggccggc gtgacggccg ttgcgagcac gtccgaccag gccgtcgcgc gcttgcgcag
     4801 caacgccggc tccgccgtga cgatctcggc gtcgccgaag tcgaccgtct cgagcgcgcg
     4861 cctgaacgtg gcggtctgct cggcgaacac cacggcgggg cgcaccagcg acaccacgtg
     4921 acgcaggcgg ccgaagtcac ccggcatcag cgaatagttg gcactgaccg ggcaaaccgg
     4981 gacgcgcgcc gccatggcgc cgaacttgat caccgcgtgc gccaccgagt tgcccgagag
     5041 gatcagcagc gagcgatccg ccggcaacga cctcgcgagc agccactgcg tgaccgcatc
     5101 ggctgcgtgc ttgaact
//
LOCUS       DASPFR010000039         3567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1478885, whole genome shotgun sequence.
ACCESSION   DASPFR010000039 DASPFR010000000
VERSION     DASPFR010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478885"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..744)
                     /locus_tag="VD737_00920"
     CDS             complement(82..744)
                     /locus_tag="VD737_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXW09150.1"
                     /translation="MGIAVVTGANRGIGLELVRQLAARGTETVAVCRHRSPELDALGV
                     QVEDDCDVTRPETWARLASRFARSDIDLLVQNAGMLVEDGLDDAQPDDVRAQFELNAL
                     APLFLTRALRSRLRTGAKVALVTSRMGSMADNGSGGYYGYRMSKAALNAAGVSLAIDL
                     KAQGVAVAILHPGAVRTGMTGGSGMIDADEAVRGLLRRIDELTLDRSGRFLHQNGQSL
                     PW"
     gene            825..1790
                     /locus_tag="VD737_00925"
     CDS             825..1790
                     /locus_tag="VD737_00925"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HXW09151.1"
                     /translation="MRRPRETGLRRAALRAALAALVLSARAFAEPAPSSEAETAPIEE
                     VLVTAPEPRYVAPTTRDGIGRIWAPVLIDGRGPFRLVLDTGATRSALIPRVAAQLGLE
                     TRPNGARVRGVTGTAVVPTVKVRSLELGELRVEDVTLPIVNDVFGGADGVLGGDGLSD
                     KRIVIEFRRDRIDIARSRRQAAPDGYTVVPLDYVRARGLRTQVMVGPVPTIAIIDTGG
                     QATVGNLALHAALSRRRGELDPFDDAIIGVTEDVQPAMRVRIPSIVAGEMIVRRAEIR
                     FADLHIFEHWKLTARPALLIGMDVLGRLDTLILDYRREEMQVKMR"
     gene            1889..3538
                     /locus_tag="VD737_00930"
     CDS             1889..3538
                     /locus_tag="VD737_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09152.1"
                     /translation="MHQRLILIAVVSVLIGFGPAPVRASAADLGRAETAFLDHLDAVG
                     AVGYLESGGAARYHDRDLAAWKARQASTRRGLEVALQALDRGAERTDEERRALAAMRR
                     TLAGLDAGESMDAGPAPRCADASNRDLAEADLRAALVGCFVEHGNHLRHAGGEIDRGT
                     ALQLLHVVDDPAQRKAIFDAFRPLWQALDGGGGADSPYRRMIALASAAARREGSEIDA
                     AARALGVDSATVERWLVQILEAWRDANPGPPIEPWDFRYVHGGANRELQPHVPVSLLL
                     PANRRYYADLGADLDALRVVLDLEPRPDKSPLAYSDFLERGRLVDGHWRRPRARVLGS
                     YSTGGLFSLNELVHENGHAVHVSAIHARPAYMDWPDTLFVEAFADVTSWSVHEPAWQR
                     KYLGHATDEAGSLRALFANVVLDVAWSLFELRMLRDPAVDPNLAWTDITQRYLRIAPH
                     PGVPWWAMRVQLVDQPGYMVNYGLGAVLTAELREHVASGVGAFDTGNARWYPWLSERL
                     LRYGSERDTRSLMIDLLGRPVSPDALLRQMRRMAPKDASNR"
BASE COUNT          488 a         1259 c         1288 g          532 t
ORIGIN      
        1 ctgtgggtca gcgtgctggc gcttgcggtg gtgacggtcg ccgggttggt ccgcctccgc
       61 cgggcgtgaa ctgcggcgcg gctaccaggg cagcgattgg ccgttctggt gcaggaaacg
      121 gccgctccgg tcgagcgtga gctcgtcgat ccgccgcagc aggccgcgca cggcttcgtc
      181 ggcgtcgatc atcccggacc cgcccgtcat cccggtgcgg accgcaccgg ggtgcaggat
      241 cgcgaccgcg acgccctgtg ccttcaggtc gatcgcgagc gacacgccgg ccgcgttgag
      301 cgccgccttg ctcatgcgat acccgtaata gccgcccgag ccgttgtccg ccatcgaacc
      361 catgcggctc gtgaccagcg cgaccttggc gccggtgcgc aaccgcgacc gcagcgcacg
      421 cgtgaggaac agcggcgcca gcgcattgag ctcgaactgc gcgcgcacgt cgtcgggttg
      481 ggcgtcgtcg agcccgtcct cgaccagcat cccggcgttc tgcaccagca ggtcgatgtc
      541 cgagcgcgcg aagcgcgacg cgagtcgcgc ccacgtctcc ggccgggtga cgtcgcagtc
      601 gtcctcgacc tgcacgccga gcgcatcgag ctccggcgat cggtggcgac agacggcgac
      661 ggtttccgtg ccgcgcgccg cgagctgccg gaccagctcc aggccgatgc cgcgattggc
      721 gccggtgacg actgcgatgc ccatgggacc tcctcgtggc ggcgtgtgcc cgtcggtcga
      781 cgggccgtcg attctagtcc cgggcaaggt gctatacgtg cgtcatgcga cgaccgcgcg
      841 aaaccgggct ccgacgtgcc gcgctgcgcg ccgcgctcgc cgcgctggtg ctgtcggcgc
      901 gtgccttcgc cgaaccggcg ccttcctccg aggcggagac cgctccgatc gaggaagtcc
      961 tggtcaccgc gcccgagcca cgctacgtcg cgccgaccac gcgggacggc atcggccgca
     1021 tctgggcgcc ggtgctgatc gacggacgcg gtccgttccg gctcgtgctc gacaccggcg
     1081 cgacccgctc cgcactgata ccgcgcgtgg cggcgcagct cggactcgag acccggccga
     1141 acggcgcccg ggtgcgcggc gtcaccggca ccgccgtcgt gcccacggtc aaggtccggt
     1201 cgctggagct gggcgagctc cgcgtcgagg acgtcacgct gcccatcgtc aacgacgtct
     1261 tcggcggcgc ggacggcgtg ctcggcggcg acgggctgag cgacaagcgc atcgtgatcg
     1321 agttccgccg ggaccgcatc gacatcgcgc gctcgcgcag gcaggcggcg ccggacggct
     1381 atacggtggt gccgctcgac tacgtgcgcg cccgtggact gcggacccag gtcatggtgg
     1441 ggcccgtgcc cacgatcgcg atcatcgaca ccggcgggca ggccaccgtc ggcaacctgg
     1501 cgcttcacgc ggcgctctcg aggcgacgcg gcgagctcga cccgttcgac gacgcgatca
     1561 tcggcgtgac ggaggacgta cagcccgcga tgcgggtgcg catcccgagc atcgttgccg
     1621 gggagatgat cgtgcgccgc gccgagatcc gcttcgcgga cctgcacatc ttcgaacact
     1681 ggaagctcac cgctcgaccc gcgctgctga tcggcatgga cgtgctcggg cggctcgaca
     1741 cgctgatcct cgattaccgg cgcgaggaga tgcaggtgaa gatgcggtga ggagaagtgg
     1801 ccagtgggta gtggtcagtg gctagtggtg acggacggat aaggtcgcgc gcgggtgcag
     1861 cctccagacc tttggatatg atcggaacat gcaccagcgg ttgatcctta tcgcagtcgt
     1921 ctccgtgctg atcggcttcg gtcccgcgcc ggttcgggct tcggccgcgg acctcgggcg
     1981 cgccgagacg gcgttcctgg atcacctcga cgcggtcggc gcggtcggct atctggaatc
     2041 cggcggcgcg gcgcgctacc acgatcgtga cctcgccgcg tggaaggcgc ggcaggcctc
     2101 gacccgacgc gggctcgaag tcgcgttgca ggcgctcgac cgcggggccg agcggaccga
     2161 cgaagaacgt cgtgcgctgg ccgcgatgcg gcgcacgctg gcagggctcg acgccggtga
     2221 gtcgatggac gccgggcccg cgccgcggtg cgcggacgcc tcgaatcgcg acctggcaga
     2281 ggccgacctg cgcgcggcgc tcgtcggctg tttcgtcgag cacggcaacc acctgcgcca
     2341 cgccggcggc gagatcgatc gcggcacggc gctgcagctg ctgcacgtcg tcgacgaccc
     2401 agcgcagcgc aaggcgatct tcgacgcgtt ccggccgctc tggcaggcgc tcgacggcgg
     2461 gggaggtgcc gacagtccgt accggcgcat gatcgcgctc gcctccgcgg cggcgcggcg
     2521 cgaaggatcg gagatcgacg cggccgcgcg tgcgctcggc gtcgactcgg cgacggtcga
     2581 gcgctggctc gtgcagatcc tcgaggcgtg gcgcgacgcc aatcccggac cgccgatcga
     2641 accgtgggac tttcgttacg tccacggagg cgccaaccgc gaactgcagc cgcacgtgcc
     2701 cgtgtccctg ctgctgccgg ccaaccgccg ctactacgcc gacctcggcg ccgacctcga
     2761 cgcgttgcgc gtggtgctcg acctcgaacc gcggcccgac aagtcgccgc tggcctactc
     2821 cgacttcctc gagcgcggac gcctggtgga cggccattgg cggcgaccgc gggcgcgcgt
     2881 gctcggcagc tattcgacgg gtgggttgtt ctcgctcaac gaactcgtgc acgagaacgg
     2941 gcacgccgtg cacgtgagcg cgatccacgc gcgtccggcg tacatggact ggccggacac
     3001 gctgttcgtc gaggcgttcg ccgacgtgac gtcctggagc gtgcacgagc cggcctggca
     3061 acggaagtac ctcggccacg ccaccgacga agccggctcg ctgcgggcgc tgttcgcgaa
     3121 cgtcgtgctc gacgtcgcct ggtcgttgtt cgagctgcgc atgctgcgcg acccggcggt
     3181 cgatccgaac ctcgcctgga cggacatcac gcagcgatac ctgcgcatcg ctccgcaccc
     3241 gggcgtgccg tggtgggcga tgcgcgtgca gctcgtggac cagccgggat acatggtcaa
     3301 ctacggcctc ggggcggtgc tcacggccga gctgcgcgag cacgtggcct ccggcgtcgg
     3361 cgccttcgac accggcaatg cgcgctggta tccgtggttg agcgagcggc tgctgcgcta
     3421 cggctccgag cgcgacacgc gctcgctgat gatcgacctg ctcggtcgcc cggtgtcgcc
     3481 cgacgcgttg ctgcggcaga tgcggcgcat ggcgcccaag gacgcgtcga accgctgagt
     3541 tgtgccatca tccgggtcct acccgag
//
LOCUS       DASPFR010000040         8211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1600677, whole genome shotgun sequence.
ACCESSION   DASPFR010000040 DASPFR010000000
VERSION     DASPFR010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8211
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600677"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1766
                     /locus_tag="VD737_00935"
     CDS             <1..1766
                     /locus_tag="VD737_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXW09153.1"
                     /translation="GAVLEPWLTDQWYVRIAPLAEPAIAAVEDGRIRFVPDNWAKTYF
                     QWMRNIQDWCISRQLWWGHRIPAWYDADGNVYVARSESDARQQAQARHGRAVDLTRDE
                     DVLDTWFSSALWPFSTLGWPRPTPELATFYPTSVLVTGFDIIFFWVARMIMMGLEFTG
                     EVPFREVYITGLIRDEHGNKMSKSKGNIIDPLDLIDGIDLEALVAKRTSGLMQPQMRA
                     SIDKATRKQFPQGIPAFGTDALRFTFAALATMGRDIRFDLGRVEGYRNFCNKLWNAAR
                     YVLMNTEEHAARLADGPGEYSVADRWIRGRLGATVTTVRDSLAGYRFDLASQAMYEFV
                     WYEFCDWYLELSKPVLQGAEATDAQRRGTRRTLLEVLEGTLRLLHPLMPFITEEIWQK
                     VGPLAGRTGATIMLQRYPTVTEFPPDAAAERDVATLQAVVLGIRQIRGELDVPHSRTT
                     PLYVRSDKAGDAEALRALAPTIRRIANLESIELVESEADLPPSAIAISGGRTVLAPFD
                     RLVDDVGAELARLGKRHAKTQQERDRSAAKLANASFVANAPESVVAQERERVAEFDRQ
                     LAQLAEQARRLGALAPAAAER"
     gene            1768..2733
                     /locus_tag="VD737_00940"
     CDS             1768..2733
                     /locus_tag="VD737_00940"
                     /inference="COORDINATES: protein motif:HMM:NF019346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXW09154.1"
                     /translation="MSALADTSSTGTSQVALRALIEAAVAGLNEIILGKERQVRLSLA
                     CLLARGHLLIEDIPGVGKTTLAHALARTLGLSYQRIQFTSDLLPADIIGVSVFEADTG
                     QFRFHRGPVFAQLVLADEVNRATPKAQSALLEAMEEHQVTADGQTYPLTEPFFVIATQ
                     NPVYQVGTFPLPESQLDRFLMRVELGYPAPDLERQLLLGRDRRDLLASLPAALTPQQL
                     LRLQAEVPRVHASDALLDYIQAIARYTRQAQEFEAGLSPRAVISLLRAAQSWAFVGGH
                     EGVLPEDVQAVLPAVVGHRLAARDSGRFRNASEIGQHVLGAVPVP"
     gene            2747..3745
                     /locus_tag="VD737_00945"
     CDS             2747..3745
                     /locus_tag="VD737_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HXW09155.1"
                     /translation="MPGLLQDLHARLDRRTRRWVLKRQGRDVDPVELRRNRIYILPTR
                     LGVAYAAMLFAMLLGGMNYGNNVGLALTFLLVGLGLVAMHHCHGTLSGLRLRLLAAES
                     AFVGDRVRFRWLLENPSAMSRPAIEVRRERVGGERGRPVEVPGLATATAEVRIPARRR
                     GRVPLERFVVSTAHPLGLFRAWAVVHPEHVAIAWPRPSDRALEPPLTSTDTGGAQMHT
                     PGDEDFAGLRPFQAGDSLHRVAWKAYARGQGLHTKQYAGTDVVSHVFDWDSLPGLGRE
                     ERLAQLCRWVLDAHERGEAFGLRLPGTTIDVNLGVSHRERCLNALALFEPAEPAHA"
     gene            3738..5744
                     /locus_tag="VD737_00950"
     CDS             3738..5744
                     /locus_tag="VD737_00950"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HXW09156.1"
                     /translation="MPDAALLRGARVASRSMPGAMPPVHGLGWVLGALAVAVAPHVPY
                     LPAWVSLLLLAVGAWRWAASLRGWTLAPRWLRIVIVVVATVAILGTYRTLNGIEAGTA
                     LLVLMAAAKLLETRGSRDLTVLVFIAWFLLYAALLRDQRLVQLPWLFGSAFLTTVALM
                     RVHADATAAPATNLARRSLTLLAQAVPVGLMLFLLFPRLPGPFWGIDAGSQARTGLDD
                     EMTPGDVSDLSASGEVAFRVRFRGEMPGAAQRYWRGPVLHEFDGRSWRRPRAQAFPPQ
                     EARFAGTPVEYQITLEPHDRRWVFALDLPSQWPEREAFQAYDFTLLSARTVGRVTAFE
                     LTSYPRFVAGVELPESLRRKDLALPTDGTNARSVQLGRELAARHQGDPRRIVGELLRR
                     FREEPFEYTMRPPLLADNAVDEFLFETRRGFCEHYASAFTVVMRAAGVPARVVTGYQG
                     GEFNPFGGYLIVRQSDAHAWSEVWVEGRGWLRVDPTAAVAPERIEGSLIDAVGADEPV
                     PGRLRDASPFVMRLELGWDAINDFWNQRVVRFDADAQFDLLQRLGIDEPDWRALGLGL
                     AASLAAFFAGLSGYLAWRYRPPPRDWPARLHDVVARRLRKRGLVRGPAEGPIAFLQRA
                     EQAQPDLARPLAEIRVLYTAQRYGPSPRATDLQRLKHLVNALRP"
     gene            5910..6176
                     /locus_tag="VD737_00955"
     CDS             5910..6176
                     /locus_tag="VD737_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HXW09157.1"
                     /translation="MADIDQKFAAAAQDIQKLTRRPDDEDMLRLYALYKQATEGDVQG
                     DRPAAFALKDRAKYDAWARVKGTDPAKAREQYVKLVERLRKTYG"
     gene            6179..7051
                     /locus_tag="VD737_00960"
     CDS             6179..7051
                     /locus_tag="VD737_00960"
                     /inference="COORDINATES: protein motif:HMM:NF017932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HXW09158.1"
                     /translation="MTAAIAAILAVAGAVLLGLLLPPLLRRRRRREARNTPLAPSMQA
                     AIERHVPIAAALPPELRRRLEGLVVAFLQEKQFVGCNGLEVTDEMRAAIAAQACLLLL
                     GRRSDVLYDELRSILVYPGAFLVEDEVHDEDGLVTRRRRELSGEAWDAHRVILSWEDV
                     AEAAARPAEGYNVVLHEFAHHLDAEGLGLANATRNASTWHEQLLDEYEQHRAAVERGE
                     DTWLDPYGAEDETEFFAVVTEEFLGCPGELREAHPRLYSLMQDFYGIDPAAWREPVAG
                     RQAGGRSRAPDTPR"
     gene            7080..7958
                     /locus_tag="VD737_00965"
     CDS             7080..7958
                     /locus_tag="VD737_00965"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXW09159.1"
                     /translation="MDLRLTMALAALVAATAVAQPDTVMTLEQALRQIQHGQGGDAIG
                     VLQRLAEDDDVRAQLALGAALIEGKVVPADRIQGYSWLQIAWLGYPGTSGYTASQGAA
                     RDLVQRAERSLSGPELIQGELRGREFRERRTAEWKAAVARARATLLAAREAGRPPARV
                     AEPTARGKSVEVTAGCALDPSRSGCTVFTTTKDFANARCDESPAASPDVIASVARAEP
                     PPLRKRPPVTCSTRYQAQLDRHGYVCSLALMDGCGHAELERTILDSVARWRFVPAMKN
                     GQPVASSHVGGVTVRR"
BASE COUNT         1171 a         2929 c         2885 g         1226 t
ORIGIN      
        1 ccggcgccgt gctcgagccg tggctcacgg accagtggta cgtgcgcatc gcgccgctcg
       61 cggaaccggc gatcgctgcg gtcgaggatg gccgcatccg tttcgtgccg gacaactggg
      121 cgaagacgta cttccagtgg atgcgcaaca tccaggactg gtgcatcagc cgccagctct
      181 ggtggggcca ccgcatcccg gcctggtacg acgccgacgg caacgtctat gtcgcgcgct
      241 cggagtccga cgcgcggcag caggctcagg cgaggcacgg acgcgccgtc gacctgaccc
      301 gggacgagga cgtgctcgac acctggttca gctccgcgct ctggccattc tcgacgctgg
      361 gctggccgcg gccgacgccg gagctcgcga cgttctatcc gacgtcggtg ctggtgacgg
      421 ggttcgacat catcttcttc tgggtggccc ggatgatcat gatgggcctc gagttcaccg
      481 gcgaggtgcc gttccgcgag gtctacatca ccgggctcat ccgtgacgag cacggcaaca
      541 agatgtcgaa gtcgaagggc aacatcatcg acccgctcga cctgatcgac ggcatcgacc
      601 tcgaggcgct cgtcgccaag cgcacgagcg ggctgatgca gccgcagatg cgcgcgtcga
      661 tcgacaaggc gacgcgcaag cagtttccgc agggcatccc ggcgttcggc accgacgcgc
      721 tgcgattcac gttcgcggcg ctcgcgacga tgggacgcga catccgcttc gacctcggcc
      781 gcgtcgaggg ctaccgcaac ttctgcaaca agctgtggaa cgcggctcgc tacgtgctga
      841 tgaacaccga ggagcacgca gcccggctcg cggacggccc tggcgaatac tcggtcgcgg
      901 atcgctggat tcgcgggcgc ctcggcgcca ccgtcaccac cgtgcgcgac agcctcgccg
      961 gctaccggtt cgaccttgcc tcgcaggcga tgtacgagtt cgtctggtac gagttctgcg
     1021 actggtacct cgagctctcc aagccggtgc tgcagggcgc cgaggcgacc gacgcgcagc
     1081 gtcgcggcac gcggcgcacg ctgctcgagg tgctcgaggg gacgctgcgc ctgctgcacc
     1141 cgctgatgcc gttcatcacc gaggagatct ggcagaaggt cgggccgctc gccgggcgca
     1201 ccggcgcgac gatcatgctg cagcgctatc cgacggtgac cgagttcccg ccggatgccg
     1261 cggccgagcg cgacgtcgcc acgctccagg cagtcgtgct gggcatccgc cagatccgtg
     1321 gcgaactcga cgtgccgcac tcgcgcacca ccccgctcta cgtccgcagc gacaaggcgg
     1381 gcgatgccga ggcgctgcgc gcgctcgcgc cgaccatccg ccggatcgcc aacctcgagt
     1441 ccatcgaact cgtggaatcc gaggcggacc tgccgcccag cgcgatcgcg atcagcggcg
     1501 gccgcaccgt gctcgcgccg ttcgaccggc tcgtcgacga cgtcggcgcc gagctcgccc
     1561 ggctcgggaa gcgtcacgcg aagacgcagc aggagcgcga ccggagtgcc gcgaagctcg
     1621 ccaacgcgag cttcgtcgcg aacgcgccgg agagcgtcgt cgcccaggag cgcgagcgcg
     1681 tcgccgagtt cgaccggcag cttgcccagc tcgccgagca ggcgcggcgg ctcggggcgc
     1741 tcgcgccggc ggcggcggag cgctgacatg agcgccctgg ccgacacctc gagcaccggc
     1801 accagccagg tcgccctgcg cgcgctgatc gaagcggcgg tcgccggcct caacgagatc
     1861 atcctcggca aggaacgcca ggttcgcttg agcctcgcgt gcctgctggc gcggggccac
     1921 ctgctgatcg aggacatccc gggcgtcggc aagaccacgc tcgcgcacgc gctcgcgcgc
     1981 acgctcggcc tcagctacca gcggatccag ttcaccagcg acctgctgcc ggccgacatc
     2041 atcggcgtat ccgtattcga ggccgacacc ggccagttcc gcttccatcg cggcccggtg
     2101 ttcgcccagc tcgtgctcgc cgacgaggtc aatcgcgcga cgccgaaagc gcagagcgcg
     2161 ctgctcgaag cgatggagga gcaccaggtc acggccgatg ggcagacgta tccgctgacc
     2221 gagccgttct tcgtgatcgc gacgcagaac cccgtctacc aggtcggcac gttcccgctg
     2281 cctgagtcgc aactggaccg cttcctgatg cgcgtcgagc tcggctatcc cgcccccgac
     2341 ctcgagcgac agttgctgct cggccgcgac cggcgcgacc tgctggcgtc gctgccggcg
     2401 gcgctgacgc cgcagcagct gctgcgcctg caggccgagg taccgcgcgt gcacgcctcc
     2461 gacgcgctgc tcgactacat ccaggcgatc gcgcgctaca cgcggcaggc gcaggagttc
     2521 gaggccggcc tgtcgccgcg cgccgtgatc tcgctgctgc gcgccgcgca gtcctgggcg
     2581 ttcgtgggtg gccacgaagg cgtgctgccg gaggacgtgc aggcggtgtt gccggccgtg
     2641 gtcgggcacc ggctcgcggc gcgggactcg ggccgcttcc gcaatgcgag cgaaatcggc
     2701 cagcacgtgc tcggcgccgt ccccgtgccc tgatcgcgac gacgcgatgc ccggcctgct
     2761 gcaggacctg cacgcgcggc tcgaccgacg cacgcgtcgc tgggtgctga agcgccaggg
     2821 ccgcgacgtc gatcccgtcg agctgcgccg caaccgcatc tacatcctgc cgacgcggct
     2881 cggcgtcgcc tatgccgcga tgctgttcgc gatgctgctc ggcggcatga actacggcaa
     2941 caacgtcggg ctcgcgctga cgttcctgct cgtcggcctg gggctcgtcg cgatgcacca
     3001 ctgccacggc acgctctcgg gcctgaggct gcgcctgctg gccgcggagt ccgccttcgt
     3061 cggcgaccgc gtgcgcttcc gctggctgct cgagaatccg tcggccatgt cgcggccggc
     3121 gatcgaggtg cgacgggaac gcgtcggcgg cgagcgcgga cgtcccgtcg aggtgccggg
     3181 cctcgcgacg gcgacggccg aagtgcggat ccccgcgcgg cgccgcggtc gcgtaccgct
     3241 cgagcggttc gtcgtttcga cggcgcatcc gctcgggctg ttccgggcct gggcggtggt
     3301 ccaccccgag cacgtcgcga tcgcctggcc gcgaccgagc gaccgcgcgc tcgagccgcc
     3361 gctcacgtcg accgacaccg gcggcgcgca gatgcacacg cccggcgacg aggacttcgc
     3421 gggacttcgt ccgttccagg ccggcgactc gctgcaccgc gtcgcctgga aagcctatgc
     3481 gcgcggccag ggcctgcaca ccaagcagta cgcgggcacc gacgtggtct cgcacgtgtt
     3541 cgactgggac agcctgcccg gcctcggccg cgaggagcgg ctcgcgcagc tctgccgatg
     3601 ggtgctcgac gcgcacgaac gcggcgaggc cttcgggttg cggctgccgg gcacgacgat
     3661 cgacgtcaac ctcggcgtgt cgcaccgcga gcggtgcctg aacgcgcttg cgctgttcga
     3721 accggcggag cccgcgcatg cctgacgccg cgttgctgcg cggagcacgc gtcgcgtcgc
     3781 gctcgatgcc cggcgcgatg ccgccggtgc acggtctcgg gtgggtgctc ggggcgctgg
     3841 cggtcgcggt cgcgccgcac gtgccgtacc tgcccgcgtg ggtgagcctg ctgctgctcg
     3901 ccgtgggcgc ttggcgctgg gccgcgagcc tgcgcggctg gacgctcgcg ccgcgctggc
     3961 tgcgtatcgt gatcgtcgtg gtcgcgaccg tggcgatcct cggcacctat cgcacgctga
     4021 acggcatcga ggccggcacc gcgctgctcg tgctgatggc cgcggccaag ctgctcgaga
     4081 cgcggggctc gcgcgacctg accgtgctcg tcttcatcgc ctggttcctg ctctacgcgg
     4141 cgctgctgcg cgaccagcgg ctggtgcagc tgccgtggtt gttcggcagc gcgttcctga
     4201 ccaccgtcgc gctgatgcgc gtgcacgcgg acgcgaccgc cgcgcccgcc accaacctgg
     4261 cgcgccgctc gctgacgctg ctcgcccagg ccgtcccggt cgggctcatg ctgttcctgt
     4321 tgtttccgcg gctgccggga cctttctggg gcatcgacgc cggcagccag gcgcgcaccg
     4381 ggctcgacga cgagatgacg cccggcgacg tctcggacct gagcgcctcg ggcgaggtcg
     4441 cgttccgcgt gcgctttcgc ggcgagatgc cgggcgccgc gcagcggtac tggcgcggcc
     4501 cggtgctgca cgagttcgac gggcgcagct ggcgccgccc gcgcgcgcag gcctttccgc
     4561 cgcaggaggc acgcttcgcc ggcacgccgg tcgaatacca gatcacgctc gaaccgcacg
     4621 accggcgctg ggtattcgcg ctcgacctgc cgtcgcaatg gcccgagcgc gaagcgttcc
     4681 aggcgtacga cttcacgttg ctctcggctc gcacggtcgg tcgcgtgact gcgttcgagc
     4741 tgacttcata cccgcgcttc gtcgccggcg tggaattgcc cgagtcgctg cgacgcaagg
     4801 acctcgcgct gccgacggac ggcaccaacg cgcgctcggt gcagctcggg cgcgagctgg
     4861 cggcgcggca tcaaggcgac ccacgccgga tcgtcggcga gctgttgcgt cgcttccgcg
     4921 aagagccgtt cgagtacacg atgcggccgc cgttgctcgc ggacaacgcc gtggacgagt
     4981 tcctgttcga gacccgccgc ggcttctgcg aacactatgc gtcggcgttc accgtggtca
     5041 tgcgcgcggc cggcgtgccc gcacgcgtcg tcacggggta ccaggggggc gagttcaacc
     5101 cgttcggcgg ctacctgatc gtgcgccagt cggacgccca cgcgtggtcc gaggtctggg
     5161 tcgagggccg cggctggctg cgcgtcgatc cgacggcggc ggtcgccccg gagcgcatcg
     5221 aaggcagcct gatcgacgcc gtgggcgccg acgagccggt gccaggccgg ctgcgggacg
     5281 cgagcccgtt cgtgatgcgg ctggaactcg gctgggacgc gatcaacgat ttctggaacc
     5341 agcgcgtcgt gcgcttcgat gccgacgcgc agttcgacct gctgcagcgc ctcggcatcg
     5401 acgagcccga ctggcgggcg ctcggactcg ggctcgcggc cagcctcgcc gcctttttcg
     5461 ccggactgtc gggttacctg gcgtggcgct atcggccgcc gccgcgcgac tggccggcgc
     5521 gcctgcacga cgtcgtcgca cgccgcctgc gcaagcgggg cctcgtgcgg gggccggccg
     5581 aaggcccgat cgcgttcctg cagcgggcgg agcaggcgca gccggacctc gcgcggccgc
     5641 tcgccgagat ccgcgtgctg tacacagccc aacgttacgg cccctccccc cgcgcgacgg
     5701 acctgcagcg tctcaagcac ctggtgaacg cactccgccc gtaaatgggg acattccttt
     5761 tttcgcgagc acggctggac gacgctggcg acgctgggcc gacgtcgcga gcgaaagcag
     5821 gagtgtcccc atttacggtg ggtagtgagg agtgtcccca ttcatgcttg tccccattta
     5881 tgcttgccgc tccagccagg gggaacgaca tggccgacat cgaccagaaa ttcgcggcgg
     5941 cggcgcagga catccagaag ctcacgcgac gtcccgacga cgaggacatg ctgcggctct
     6001 acgccctcta caagcaggct acggagggcg acgtgcaggg cgaccgcccg gcggccttcg
     6061 cgctcaagga ccgcgccaag tacgacgcct gggcccgcgt gaagggcacc gacccggcca
     6121 aggcccgcga gcagtacgtc aagctggtcg agcgtcttcg caagacctac ggctgagcgt
     6181 gaccgcggcg atcgctgcca tcctggcggt ggccggtgcg gtgctcctcg ggctgctgct
     6241 gccgccgctg ctcagacggc ggcgccggcg cgaggcccgc aatacgccgc tcgccccttc
     6301 gatgcaggcc gcgattgaac gccacgtgcc gatcgccgcg gcactgccgc ccgagctgcg
     6361 ccgccggctc gagggactgg tcgtcgcatt cctccaggaa aagcagttcg tcggctgcaa
     6421 tggcctcgaa gtcaccgacg agatgcgggc ggcgatcgcc gcgcaggcgt gcctgctgct
     6481 gctcgggaga cggtccgacg tcctgtacga cgagttgcgg tcgatcctcg tgtacccggg
     6541 tgcgttcctg gtagaggacg aggtgcacga cgaggacgga ctggtcacgc gtcgccgccg
     6601 cgagctgtcg ggcgaggcct gggacgcgca tcgcgtgatc ctgtcctggg aagacgttgc
     6661 cgaagccgcc gcgcgtcctg cagaagggta caacgtcgtg ctgcacgagt ttgcgcatca
     6721 cctcgatgcc gaaggcctgg gcctggccaa tgcgacacgc aacgcctcga cgtggcacga
     6781 gcaactgctc gacgagtacg agcagcatcg cgcggccgtc gagcgcggcg aggacacctg
     6841 gctcgacccc tacggcgccg aggacgagac ggagttcttc gccgtcgtca ccgaggagtt
     6901 cctcggctgc cccggcgagc tccgcgaggc gcacccgcgg ctctactcgc tgatgcagga
     6961 cttctacggc atcgacccgg ccgcgtggcg cgagccggtg gcgggaaggc aggccggtgg
     7021 gcgttcgcgc gcccccgaca cgccacgtta gaagacaccc acacgacaca ggtgaccgca
     7081 tggacctacg gctcacaatg gcacttgcgg cactcgttgc ggcaacagcc gttgcacagc
     7141 ccgacacggt catgacgttg gagcaggccc tgcgacagat ccagcacggc cagggcggcg
     7201 atgcgatcgg ggtgctgcaa cgactcgccg aggacgacga cgtacgcgcg cagttggcgc
     7261 tcggtgccgc actgatcgag ggcaaggtcg tgccggcgga tcgaatacaa ggctactcgt
     7321 ggctgcagat cgcctggctc ggctaccccg gcacctccgg gtacaccgcc agccagggcg
     7381 ccgcccgcga ccttgtccag cgtgcagagc ggagcctgtc gggtccggaa ctgatacagg
     7441 gcgagcttcg cggccgggaa tttcgcgaac gtcggacggc ggagtggaaa gccgcggtcg
     7501 cgcgcgcgag agcaacgctg ttggcggcgc gcgaggcggg tagaccgccc gcccgagtcg
     7561 ccgagccgac cgcgcggggc aaatcagtcg aggtgaccgc ggggtgcgcg ctggatccct
     7621 cgcgttctgg gtgcacggta ttcacgacca ccaaggactt cgcgaacgcg cgctgcgacg
     7681 agtcacccgc cgcatccccg gacgtgattg caagcgtggc aagggccgag ccgcctccgc
     7741 tcagaaaacg tccacccgtg acctgcagca cgcggtacca agcgcaactc gaccgccacg
     7801 gctatgtgtg cagccttgct ctcatggacg gttgcggcca cgcggagctc gagcgaacca
     7861 tattggactc cgtcgcgcgc tggcgcttcg tcccggcgat gaagaatggc cagcccgtgg
     7921 cttcctcgca cgtcggcggc gtcaccgtgc gccgatgacc ggcgtcgctg cgaccgaccg
     7981 gacgccagcc cagtaggttc gccgtcctgg cgtagacgcc tgtctgcact cgtgtctgta
     8041 cgcgtgcgtt tccgcgtgcg cggcgagctg agtagtttcc ggttcgcgcc tgcggaacac
     8101 cgggactgcg ggaaccgtgg tcctttggaa cactgccgcg ccatccggtt cccagggatt
     8161 ccagccatgc caggccagtc gtcgtcgagc cgcgtccttg cgaaagcctg c
//
LOCUS       DASPFR010000041         2705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_746932, whole genome shotgun sequence.
ACCESSION   DASPFR010000041 DASPFR010000000
VERSION     DASPFR010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746932"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /gene="wrbA"
                     /locus_tag="VD737_00970"
     CDS             <1..378
                     /gene="wrbA"
                     /locus_tag="VD737_00970"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF002999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HXW09160.1"
                     /translation="MAAQMRNFFDQTGGLWARNALVGKVGSVFTCTASQHGGQETTIT
                     SFHTTLLHHGLIVVGLPYSFTELSSMDEIQGGTPYGASSITGAGAQMRMPTERELQMC
                     RHQGGHLARITDQFVRGAASARR"
     gene            505..1986
                     /locus_tag="VD737_00975"
     CDS             505..1986
                     /locus_tag="VD737_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HXW09161.1"
                     /translation="MAASRPIPMLDLMFLLTESVDNPRHVGSALIFRKPAQDGDRVVR
                     EIVEAYRAARPKAPFNRVPVLLRAGLPHWREVEEFDASHHVLHVALPAPGTERQLHEL
                     LADLHAPMLERHRPGWKVYVIDGLEDDRFVVYHKVHHSLVDGESGIEILRRSLADSPG
                     DRHVRTTVGLEHAAPRRAVPRGLEPLLEREVRALLRRALATGVGGLYLLEQLLGGLRG
                     YAAETPRAFTAPATPMNESIRNARSVGHAVLPLQSMKSVASRYGATVNDVALCVVDDA
                     MNRYLRERSLKPDRPLVAICPVSLRDETAREPTTYVSAIWPPLGPVDARIDERLQAIV
                     ANTLAAKAELKRLGKSVAYTYAVMAFALSETLTAANPHLHGLRPANMLVSNVRGPERP
                     LYLNGARLEALFPISTLIAGVGLNVTFMSYAGQVVFGFTGNGAALPEMETVAAHVAEA
                     LAALEHTVRPAQAAARGARPVARRTTVPPTRSRGSFRRTTPES"
     gene            1989..2147
                     /locus_tag="VD737_00980"
     CDS             1989..2147
                     /locus_tag="VD737_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09162.1"
                     /translation="MPPTCAILHPLPARWLQEAAMTGTEYWALGYLSGMLMAWLMRKT
                     ERPPRANR"
     gene            complement(2212..>2705)
                     /locus_tag="VD737_00985"
     CDS             complement(2212..>2705)
                     /locus_tag="VD737_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXW09163.1"
                     /translation="AWETMAQMFVVPPASWLLKREVTWIPIVGWAVRTYRPIAINRSA
                     GHSAVNQVVAQGRERLASGMGVVVYPEGTRVAPGQTRKYGISGALLACETGTPVVPIA
                     HNSGYFWRRRSLLKKPGTIRVVIGPPIDPTGLTPRQLNERAQQWIEETVAEITAQPGG
                     RPR"
BASE COUNT          414 a          953 c          926 g          412 t
ORIGIN      
        1 atggccgcgc agatgcgcaa tttcttcgac cagaccggcg gcctgtgggc acgcaacgcg
       61 ctcgttggca aggtcggcag cgtattcacc tgcaccgcga gccagcacgg gggccaggaa
      121 accacgatca cgtcgttcca cacgacgctg ctgcatcacg gcctgatcgt cgtcggcctg
      181 ccgtattcgt tcaccgagct ctcgagcatg gacgagatcc agggcgggac gccgtatgga
      241 gccagttcca tcaccggcgc cggcgcgcag atgcggatgc cgacggagcg cgaactgcag
      301 atgtgccggc accagggcgg tcaccttgcg cggatcacgg accagttcgt gcggggcgcg
      361 gcatccgcac gccgctagct gcgttggcgc ggatgcgtac tgctcccgat tgcgccaatg
      421 gcgcccgcgg ccgaaattcg cgccgttggc aaccgccacg ccgttcctcg ccagcgcggg
      481 acccgggcgg ggcagagagg cgccatggct gcgagccgcc cgatcccgat gctggacttg
      541 atgtttctcc tgacggagag cgtcgacaat ccgcgtcacg tgggctcggc gctgatcttc
      601 cgcaagcccg cgcaggacgg cgatcgcgtg gtccgcgaga tcgtcgaggc gtaccgggcc
      661 gcccgcccga aggcgccgtt caaccgcgtg ccggtcctgc tgcgggcggg gctgccgcac
      721 tggcgtgaag tcgaggagtt cgacgcgagt caccacgtcc tgcacgtcgc actgccggcg
      781 cccggaaccg agcgccagct ccacgagctg ctcgcagacc tgcacgcccc gatgctcgaa
      841 cggcaccggc cgggctggaa ggtctatgtg atcgacggtc tcgaagacga ccgtttcgtg
      901 gtctaccaca aggtccatca ctccctcgtg gacggcgaat cggggatcga gatcctccgc
      961 cgttcgctcg ccgactcgcc gggcgaccgg cacgtccgca cgaccgtcgg gctcgaacac
     1021 gcggcaccgc gccgtgcagt gccgcgtgga cttgagccgc tgctggaacg tgaggtgcgg
     1081 gcgctgctcc gtcgtgccct ggcgactgga gtgggtggcc tctacctgct cgagcagttg
     1141 ctcggcggcc ttcgcggcta cgcggcggag acgccgcgcg cgttcacggc gccggcgacg
     1201 ccgatgaacg aatcaatccg caacgcgcgg tcggtcgggc acgcggtgct gccgctccaa
     1261 tcgatgaagt ccgtggcgtc gcggtacggg gcaaccgtca acgacgtggc gctctgcgta
     1321 gtcgacgacg cgatgaatcg ctacctgcgc gagcgcagct tgaagcccga ccggccgctc
     1381 gtcgcgatct gcccggtatc gctgcgcgac gagaccgcac gggaacccac cacctacgtc
     1441 tccgcgatct ggccgccgct gggcccggtc gacgcgcgca tcgacgagcg actgcaggca
     1501 atcgtggcga acacgctcgc ggccaaggcg gagctgaagc gtcttgggaa aagcgtcgct
     1561 tacacctatg ccgtcatggc gttcgcgctg tccgagacgc tcaccgctgc caatccgcac
     1621 ctgcacggcc tccggccggc gaacatgctg gtgtcgaacg tgcgcggacc ggaacggccg
     1681 ctctacctga acggcgcgcg gctcgaggca ctgttcccaa tctcgaccct gatcgcgggc
     1741 gtcggcctca acgtgacgtt catgtcgtac gccgggcagg tggtcttcgg gttcaccggc
     1801 aacggggcgg cgctgcccga aatggaaacg gtggccgcgc acgtcgcgga ggccctggcg
     1861 gcgctcgagc acaccgtacg gccagcgcag gcggcggccc gcggcgcccg tccggtcgcg
     1921 cgtcgcacca cggtgccgcc tacgcgatca cgaggttcgt tccgacgcac gacgccggaa
     1981 tcgtgagaat gccgccgact tgcgcgatcc tgcatcctct gccggcgcga tggctgcagg
     2041 aggcggcgat gaccggcacc gagtactggg cgctgggtta tctcagcggg atgctgatgg
     2101 cgtggctgat gcgcaagacc gagcgcccgc cacgcgccaa ccgttgacga ccaaccgttg
     2161 acgagatgac ccgcggagcg gctgtcgcgg cggatccacg tcccccaccg ctcagcgtgg
     2221 ccggcccccc ggttgcgcgg tgatctccgc caccgtctcc tcgatccact gctgagcacg
     2281 ttcgttgagc tggcgcggcg tgaggccggt cgggtcgatc ggcgggccga tcacgacgcg
     2341 gatcgtcccc ggcttcttca ggaggctgcg gcggcgccag aaatacccgg agttgtgtgc
     2401 aatcggcacg accggcgtgc cggtctcgca ggccagcagc gcgccgctga tgccgtactt
     2461 gcgggtctgc cccggcgcaa cgcgagtccc ttccggatac accacgacac ccatgccgga
     2521 ggcgagtcgt tcgcgtccct gcgcgacgac ctggttgacg gcggagtgcc ccgcgctgcg
     2581 attgatcgcg atcggtctgt acgtgcgcac cgcccagccg acgatcggga tccacgtcac
     2641 ctcgcgcttc agcagccacg acgcgggcgg cacgacgaac atctgcgcca tcgtttccca
     2701 ggccg
//
LOCUS       DASPFR010000042         3279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_624120, whole genome shotgun sequence.
ACCESSION   DASPFR010000042 DASPFR010000000
VERSION     DASPFR010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624120"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VD737_00990"
     CDS             <1..869
                     /locus_tag="VD737_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXW09164.1"
                     /translation="IVRADGTTRYLHHIAQLTFDAAGRPLRHVGTVHDVTDRRQTEDE
                     ARQLQERLTHFSRLSTMGEMAAGLAHEINQPLSAIAMYAQAGQRFMRSPTREDADVLE
                     ALEQINAQALRAGEVIRRLRNFVKNREVKREPVDCNRLLEDLRTLAETDARLHNVRLR
                     IDDEPHLPTVYADPIQLQQVVLNLVRNAIDAMAEVPESQREIVLSARLVPEGEIEVVV
                     ADHGTGLAQDASEHLFNPFFTTKSGGTGLGLAISRSIVRAHGGRLWHTPNERTGARFH
                     FTLPVSPAPKGE"
     gene            876..1499
                     /gene="fixJ"
                     /locus_tag="VD737_00995"
     CDS             876..1499
                     /gene="fixJ"
                     /locus_tag="VD737_00995"
                     /inference="COORDINATES: protein motif:HMM:NF006900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator FixJ"
                     /protein_id="HXW09165.1"
                     /translation="MIKEIRPTVFVVDDDAAVRDSLRMLLKSVGLPVEVFESGQEFLD
                     ADRDDRPGCLVLDIRMPGMSGLELQGKLNERHSILPIIFITGHGDVPMAVEAMQAGAV
                     DFIQKPFRDQDLLDRINQALEKDAGSRRMLAERNMIRKRLESLTPREREVLDLVVAGK
                     ANKVIAGDLNLSQRTVEIHRARVMEKMEAHSLAHLVRMVLEVERAEE"
     gene            1686..2105
                     /locus_tag="VD737_01000"
     CDS             1686..2105
                     /locus_tag="VD737_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXW09166.1"
                     /translation="MPPSTSDAQEAPPPVGVIDPDPAARAGIRTLLQPVGVEVVPFES
                     AEAYLLAERDRELSCLIVELLLPGMSGLELLRRLRSLGNDVPVILLADESDVPTAVAA
                     MREGATDFIEKPYVNVAVLKQVQKLLRYDAGGGLARP"
     gene            2139..3182
                     /locus_tag="VD737_01005"
     CDS             2139..3182
                     /locus_tag="VD737_01005"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HXW09167.1"
                     /translation="MSSSNHSLDSWQEEKQSSWLYRELAQCEPDARVAELFRALAAAA
                     EFQAEKWLATSPGVSPPEFAPSVRARITAALARALTPRRVRHVLAAMKVRGLSAYDSQ
                     RTLAGHLMPTSVAEVGARHKGYGGGSLRAAVFGINDGLVSNTSLIMGVAGASASVDFV
                     LTSGVAGLLAGALSMAAGEYVSMRSQREMFEYQIGLERAELREYPEEEAEELALIYQA
                     RGMQLDEARRVSRELVKDEANALDALAREELGLNPDDLGSPWGAAIASFLAFAVGAVL
                     PLLPFLFGLPLTRAVVTAAVVAACALFGVGAMLSLFTGRSALAGGLRMVLIGGGAGAA
                     TWAIGSLLGAAIA"
BASE COUNT          517 a         1118 c         1129 g          515 t
ORIGIN      
        1 ggatcgtgcg cgccgacggc accacgcggt acctgcacca cattgcgcaa ctgacgttcg
       61 acgctgcagg ccgtccgctg cggcacgtgg gcaccgtcca cgacgtgacc gatcgccgcc
      121 agaccgagga cgaggcgcgg caactgcagg agcgactcac gcatttcagc cggctctcga
      181 ccatgggcga gatggccgcc gggctcgcgc acgagatcaa ccagccgctc tcggccatcg
      241 cgatgtacgc acaagcgggg cagcgtttca tgcgctcgcc cacccgcgag gacgccgacg
      301 tgctcgaggc gctcgagcag atcaacgcgc aggcgctgcg cgcgggcgaa gtcatccgcc
      361 ggctgcgcaa cttcgtcaag aaccgcgagg tcaagcgcga accggtcgac tgcaaccggc
      421 tgctcgagga cctgcgcacg ctggcggaaa ccgacgcgcg gctgcacaac gtgcggctgc
      481 ggatcgacga cgagccgcac ctgccgaccg tctacgccga tccgatccag ctgcagcagg
      541 tcgtgctgaa cctcgtgcgc aacgcgatcg acgcgatggc cgaggtgccc gaatcgcagc
      601 gcgagatcgt gctgagcgca cgcctggtgc cggagggcga aatcgaagtc gtcgtcgccg
      661 accacggcac cggcctcgcg caggacgcgt ccgagcatct gttcaacccg tttttcacga
      721 ccaagtcggg tggcaccggg ctcggcctcg ccatcagccg gtccatcgtg cgagcccatg
      781 gcgggcggct gtggcacact ccgaacgagc ggacgggtgc gcgttttcac ttcacgctgc
      841 cggtgtcgcc ggcgcccaaa ggggagtagg ttcgagtgat caaggaaatc aggcccacgg
      901 tgttcgtcgt cgacgacgac gcggcggtgc gcgattcgtt gcggatgctg ctcaagtccg
      961 tcggcttgcc ggtcgaggtc ttcgagtcgg gccaggagtt cctcgacgcc gatcgtgacg
     1021 accgtcccgg ctgcctcgtg ctcgacatcc gcatgcccgg catgagcggc ctcgagttgc
     1081 agggcaagct caacgagcgc cactcgatcc tgccgatcat cttcatcacc gggcacggcg
     1141 acgtgccgat ggccgtcgag gcgatgcagg ccggtgccgt cgacttcatc cagaagccgt
     1201 tccgcgacca ggacctgctc gaccgcatca accaggcgct cgagaaggac gcgggcagcc
     1261 ggcgcatgct tgcggagcgc aacatgatcc gcaagcgcct cgagtcgctg acgccgcgcg
     1321 agcgcgaagt gctcgatctc gtcgtcgccg gaaaagccaa caaggtgatc gcaggcgacc
     1381 tgaacctgtc acagcgcacc gtcgagatcc atcgcgctcg cgtgatggag aagatggaag
     1441 cgcactctct cgcgcacctg gtgcgcatgg tgctcgaggt cgagcgagcc gaagaatagg
     1501 gcttgctgcg gcgcacaaga acgcgcctga gaagcacttt ggtttaggta taagtcccca
     1561 tttgccggcg cggtcagcgc cggaaaactg cgttcatgcg gcttcctcgc gcaacgcagc
     1621 tcgccttctt cgaaccaccc ggcggctcgt cttagccgca cggcttcgtg ctcgtccccc
     1681 gctccatgcc accctcgacc tccgacgcac aggaggctcc gccgccggtc ggagtcatcg
     1741 atcccgatcc cgcggcacgg gccggtatcc gcacgctgct gcagcctgtt ggcgtcgagg
     1801 tcgtgccttt cgagagcgcc gaagcctacc tgcttgcgga gcgcgaccgg gagctgtcct
     1861 gcctgatcgt cgagctgctg ctgccgggca tgagcggcct cgagctgctg cgacggctgc
     1921 gatcgctcgg caacgacgtg ccggtgatcc tgctcgcgga cgagtctgac gtgccgacgg
     1981 ccgtcgcggc gatgcgcgaa ggcgccaccg acttcatcga gaagccgtac gtcaacgttg
     2041 ccgtgctcaa gcaggtgcag aagctgctgc gctatgacgc gggcggcggc ctcgcgcgac
     2101 cctgacgggc tgctaagctc gcgcctgcag gcaggcgcat gagcagcagc aaccattccc
     2161 tcgacagctg gcaggaggaa aagcagtcct cctggcttta ccgcgagctc gcacagtgcg
     2221 agcccgacgc acgtgtcgcg gagctgttcc gtgcactggc ggcggccgcg gagtttcagg
     2281 cggagaagtg gctcgccacg tcacccggtg tctccccgcc cgagttcgcg ccgagcgtac
     2341 gtgcgcggat cacggcggcg ctcgcacgcg cgctgacgcc gcgacgcgtg cgtcacgtgc
     2401 tggccgcgat gaaggtacgc gggctctcgg cctacgactc gcagcgcacg ctcgcgggtc
     2461 acctgatgcc gacgtccgtc gcggaagtcg gtgcgcgtca caagggatat ggcggtggca
     2521 gcctgcgtgc cgccgtgttc gggatcaacg acgggctggt ctccaacacg agcctgatca
     2581 tgggcgtcgc gggcgcttcg gcgagcgtcg acttcgtgct gacgagtggt gtcgccgggc
     2641 tgctcgccgg ggcgctgtcg atggccgcgg gtgagtacgt ctcgatgcgc tcgcagcgcg
     2701 agatgttcga gtaccagatc gggctcgagc gcgccgagtt gcgcgagtac cccgaggagg
     2761 aagccgagga actcgcgctg atctaccagg cgcgcggcat gcagctcgac gaggcgcggc
     2821 gcgtcagtcg cgagctggtc aaggacgaag ccaacgcgct cgacgcgctc gcacgcgagg
     2881 aactcgggct caatccggac gacctcggct cgccctgggg cgcggccatc gcctcgttcc
     2941 tggccttcgc ggtcggcgcc gtcctgccgc tgttgccgtt cctgttcgga ttgccgctca
     3001 cgcgcgcagt cgtgacggcc gcggtcgtcg ccgcctgcgc gctgttcggc gtcggcgcga
     3061 tgctgagcct gttcaccggt cgcagtgcgc tcgcgggcgg cctgcgcatg gtgttgatcg
     3121 gcggcggcgc cggcgcggcg acgtgggcga tcgggtcgct gctcggggcg gcgatcgcct
     3181 gagtgacgcc gaacgctcga atcaggggcg ccgatccggc cattcggcat tcattcgcaa
     3241 ggacgccgta aacccgtccc tgggggctcc gcgcgcaac
//
LOCUS       DASPFR010000043         7721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_136400, whole genome shotgun sequence.
ACCESSION   DASPFR010000043 DASPFR010000000
VERSION     DASPFR010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136400"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..484)
                     /locus_tag="VD737_01010"
     CDS             complement(77..484)
                     /locus_tag="VD737_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXW09168.1"
                     /translation="MTRPVARTTFATGTPWEPVVGYSRAVRVGSMVFVSGTTATGPDG
                     GLVGRGDAGAQARQVLANLRAALERAGARLEDVVRTRIYVTDISQWEAIGRAHGEVFG
                     AIRPATAMVEVSRLIDPDMLVEIEADAVIVDAG"
     gene            complement(481..1002)
                     /locus_tag="VD737_01015"
     CDS             complement(481..1002)
                     /locus_tag="VD737_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXW09169.1"
                     /translation="MNTGLPTLSIDPATPADVPLLLSLIRELAEYERLLHDVRIRPEQ
                     LQRHLFGPRPYAEAVVARADDEPVGFALWFHNYSTFEGRPGLYLEDLFVRPAHRGRGY
                     GEALLRHLARLAVERDCARFEWAVLDWNEPAIRFYRKLGAVAMDDWTVQRVAGDALRA
                     LAGLAASTGGSTS"
     gene            1095..2021
                     /gene="gcvA"
                     /locus_tag="VD737_01020"
     CDS             1095..2021
                     /gene="gcvA"
                     /locus_tag="VD737_01020"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HXW09170.1"
                     /translation="MATPPDRIPLNALRVFEAVASHLSFTAAARELGVTTAAVSAQIK
                     TLEADIGVPLFRRHTRAVALTQEGAELLPGVRRGLDELTRAVERIRRERVGGMLNLSV
                     PSEFLQRWLLPRLGEFTRQHPEIEVRFGPSRATLDFGRDDFHAAILYGPGQWPGLKAE
                     RLLDEYVFPVCSPALLAKLGPIDTLADFTRYTLIHSRAEPWMDWFRRVGGDTTRVEGG
                     LPPLEDSAATLAAVEQGKGVALARWSLVAADIAAGRLARPMLPSVQQRNAWFLVAPPE
                     QFALPKVVRFRAWLLACCREFPPPAGETIDAD"
     gene            2094..2393
                     /locus_tag="VD737_01025"
     CDS             2094..2393
                     /locus_tag="VD737_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09171.1"
                     /translation="MGGYSGTRSSRLRFHAVTVMPGADACPQARSLLGVRVLSADAPK
                     LPIAGCDRPERCDCRFKHHDDRRAGQRRRVTRTPLPLGERRAVQGRRSDDWPDQD"
     gene            complement(2396..3346)
                     /locus_tag="VD737_01030"
     CDS             complement(2396..3346)
                     /locus_tag="VD737_01030"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HXW09172.1"
                     /translation="MRTANVVAHRWVEWSLAALCIVSLAVGLAPLGWPFELFAHFRWQ
                     LAAATAVLVVVVVAGRRGRRSTTLLLLAIALQATPLLWQAGTPPRLGRDCEGPALRVA
                     TVNLWYRSRDATPALAWLAAHPADVVVLQEVTAEWADVLSVTGDLYPHRATRPRQDPY
                     GIGLLSRWPIVRLDYLDFADDGRPSVVATIDVDGRHVQVIGLHTAWPVLRNLLLARDR
                     ALSRAAALARESTLPTVIAGDFNLTPYAPAFRRLERDAGMRDALAHRAWRPTWQASLW
                     PLALPIDHVLVPGGACVQSVRIGGDVGSDHRPVYVGLRWP"
     gene            3537..4490
                     /locus_tag="VD737_01035"
     CDS             3537..4490
                     /locus_tag="VD737_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09173.1"
                     /translation="MPVAADSVTVSGFSAGGYMAVQFHVAHSSLVQGAGVFAAGPYYC
                     AENSLRDALGRCMQGDDGIPADRLASLTSQLALDGEIDPIAGLASDRVYLWHGGADGV
                     VAKSVVDAAQAYYATLVTPASIARVELERAAHTFPADDASLPACDVTAEPFVGQCGYA
                     GARNMLEHLYDPLAAGASPVRPGELRVVDQAPYAKASGADGMAARGWLYVPAACAGRG
                     PPGRCRLHVVFHGCKQGASYVDDTFVRRSGYLAAADAGNVVVLFPQAEPSFQPLNPNG
                     CWDWWGYGSEAYATRRGPQVMAVKAMVDDLLGAAAAETRAE"
     gene            complement(4491..4940)
                     /locus_tag="VD737_01040"
     CDS             complement(4491..4940)
                     /locus_tag="VD737_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09174.1"
                     /translation="MGAIEFASRVPRPRRHALERLLFFNGCQERVARDIVDVIDRYGP
                     PEIYDDGEGLRVRVASLPDVQCLFAIERASQRPIGVAVYMRADLEHVTVLHLGVAEDY
                     CAGGLNESMNLPLRLVREVRRTSRRIKGVRRLEVAYCTLRARASSAY"
     gene            5159..6820
                     /locus_tag="VD737_01045"
     CDS             5159..6820
                     /locus_tag="VD737_01045"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXW09175.1"
                     /translation="MSATVKLRQLTVASPAEPETAAPGDPASGSDPRGREGVLVDVVV
                     LTADMTLFQSAREAVGERNPVWRARSAEEAADLLITGRCGVLLIDMASVSAHGQTLIG
                     QIVAQFPEVVVCVAGTREDEPSLVPLITEGLVYRFMHKPTSARRAGMFLQAAIKRHLE
                     QREGRGGDDPLALWRSVTRHGGGLPRKYFVAFALVCVGLLATLFFGGNSPDAPPSPQS
                     APLPDPIPAVATHRADPVLSRARAAMQAGRLEAPQGRNALDLFEAVLLAQPDHVEARA
                     GLDATVQRLLVRARAEAASGRKAEAERLLQRVLAVVPSHADAKTLLVEINPPDLPSRQ
                     LEREQLAEVRERAALAPAPVALSGGVRAPVAVAIDPTRPLPEPVSSAYLARLESRAAS
                     PPVATRPTVESDPLAPRYVNSAPPPRVASWRRRAPTGGVPAVAALPTAGLEQPAAPGV
                     PEPQSVAPDPTVRADAFERVHAPEPVYPAQALRNRTRGWVELEFTVTPTGSVTDVQVV
                     GAEPEGVFDAAATQALVQWRFKPRIVSGRGVAQRTSVTLRFDVDD"
     gene            complement(6792..>7721)
                     /locus_tag="VD737_01050"
     CDS             complement(6792..>7721)
                     /locus_tag="VD737_01050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXW09176.1"
                     /translation="SVFNLFVVAVFWSLMADVFTREQAGRLFGFIAAGLSLGGLFGPF
                     LGKQLVQPLGTINLLLVSAALLAVGLGFMLAVLAWHRRHGVRDPAAAGADPDARLGGG
                     ALAAFAQVAKSPYLALIAVFVLLLTWVSTFLYLEQQAMVAATFSDRDARTEFFNTLDF
                     WVQAASLTVQLFLFGRLYRWLGFRALIALVPVAMTLGFATLALMPSFAVLIGVMVVRR
                     VGEYAIARPSRDTLYTVVTREEKYKAKSLIDTFVYRGGDATSASVHALLKSTFGLGLV
                     GIAWVGAGIAAAWALVAYRLGTLQVSRRRRIEA"
BASE COUNT         1184 a         2774 c         2717 g         1046 t
ORIGIN      
        1 tcgcccacga ccgtgggctc gacgaagacg ccgctcgcgg cgaccggcga cgccagcagc
       61 gacgtccaat cgtacgctaa cccgcgtcga cgatgaccgc gtcggcctcg atctccacca
      121 gcatgtcggg gtcgatcagc cgcgacacct cgaccatcgc ggtggccggt cgaatcgcgc
      181 cgaacacctc gccgtgggcg cggccgatcg cctcccactg cgagatgtcg gtgacgtaga
      241 tccgcgttct gacgacgtcc tcgagccgcg caccggcgcg ctcgagggcc gcgcgaagat
      301 tggcgagcac ctggcgggcc tgggctccgg cgtcgccgcg acccacgagg ccgccgtccg
      361 ggccggtcgc ggtggtgccg gacacgaaga ccatcgagcc gacgcggacg gcgcgcgagt
      421 aaccgacgac aggctcccag ggggtgccgg tggcgaacgt ggtgcgagcg acgggtctgg
      481 tcacgatgtc gaacctccag tagacgccgc cagtccggcg agcgcgcgaa gcgcgtcgcc
      541 cgcgacgcgc tggacggtcc agtcgtccat cgcgacggcg ccgagcttgc gatagaaacg
      601 gatcgcgggc tcgttccagt cgagcacggc ccactcgaaa cgcgcgcagt cgcgctccac
      661 ggcgagtcgt gcgaggtgcc ggagcagcgc ctcgccgtag ccgcggcccc gatgcgcggg
      721 tcgcacgaac aggtcctcga ggtacagccc gggccgtccc tcgaacgtcg aatagttgtg
      781 gaaccagagt gcgaagccga ccggctcgtc gtcggctcgc gccaccacgg cctccgcgta
      841 gggacgcggt ccgaacaggt gacgctgcag ctgctcgggc cggattcgca cgtcgtgcag
      901 cagacgctcg tactcggcga gctcgcggat cagcgacagc aacaggggca cgtcggcggg
      961 ggtcgccgga tcgatggaga gggtcggcag gccagtgttc atttccaaga ttccctcgac
     1021 agcgcatcga agcgcgcctt gcgtgcaacg aatttcgacc gtattgtcaa ccatcgattc
     1081 actgaggcac ctggatggct accccaccgg atcgcatccc gctgaacgcg ctgcgcgtgt
     1141 tcgaagccgt cgcctcgcac ctgagcttca ccgccgcggc gcgcgaactc ggcgtcacga
     1201 ccgccgccgt cagcgcccag atcaagacgc tcgaggccga catcggcgtg ccgctgttcc
     1261 gccgtcacac gcgggcggtc gcgctcacgc aggaaggcgc cgaactgctg cccggcgtac
     1321 gacgcgggct cgacgagctc acgcgtgccg tcgagcgcat ccggcgcgaa cgcgtcggcg
     1381 gcatgctcaa tctcagcgtg ccgagcgagt tcctgcaacg gtggctgttg ccgcggctcg
     1441 gcgagttcac gcgccagcat cccgagatcg aggtccgctt cggcccgtcg cgggcgacgc
     1501 tcgacttcgg ccgcgacgac ttccacgcgg cgatcctcta cggacccggc cagtggccgg
     1561 gcctcaaggc ggagcggctg ctcgacgaat acgtgttccc ggtctgcagt ccggcgctgc
     1621 tcgcgaagct cggaccgatc gacacgctcg ccgacttcac gcgctatacg ctgatccaca
     1681 gccgcgccga gccctggatg gactggtttc gccgcgtggg tggcgacacc acgcgcgtcg
     1741 agggcgggct gccgccgctg gaagactcgg ccgcgacgct cgccgccgtc gaacagggca
     1801 agggcgtggc actcgcgcgc tggtcgctgg tcgccgcgga catcgcggcg ggtcggctgg
     1861 cgcgacccat gctgccgagc gtccagcagc gcaatgcctg gttcctcgtg gcgccgcccg
     1921 agcagttcgc gctgcccaag gtggtgcgct ttcgtgcctg gctgctggcg tgctgccgcg
     1981 aattcccccc gccggcgggc gagaccatcg acgccgactg accggctatt ggcggccgac
     2041 gtccaaaggc gtagcgtgcc gggacgacaa cgacaagaac agggaaggcg gccatgggtg
     2101 gctactcggg gactcgctcg tcgcggttgc gtttccacgc ggtcacggtc atgcccggtg
     2161 ccgatgcgtg tccgcaggcg cgctcgctgc tcggcgtgcg cgtgctctcg gccgacgcgc
     2221 ccaagctgcc gatcgccggc tgtgaccggc ccgagcgatg cgactgtcgt ttcaagcacc
     2281 acgacgaccg tcgcgcgggt cagcgccgtc gcgtgacccg tacgccgctg ccgctcggtg
     2341 agcgtcgggc ggtgcagggc cgccgctcgg acgactggcc cgaccaggac tgacgtcacg
     2401 gccagcgcag gcccacgtag acgggccgat ggtccgagcc cacgtcgccg ccgatccgga
     2461 cggactgcac gcacgcgccg ccgggcacca gcacgtgatc gatcggcagt gcgagcggcc
     2521 agagcgacgc ctgccacgtc ggacgccatg cccgatgcgc gagcgcgtcc cgcatgcccg
     2581 cgtcgcgctc gagccggcga aacgccggcg cgtacggcgt caggttgaag tcgccggcga
     2641 tcacggtcgg gagcgtcgat tcgcgggcca gcgcggcggc gcggctgagc gcccggtcac
     2701 gtgcgagcag caggttcctg agcaccggcc aggccgtatg caggccgatc acctggacgt
     2761 ggcgcccgtc gacgtcgatg gtcgcgacga ccgacggccg cccgtcgtcc gcgaaatcga
     2821 ggtagtcgag gcgcacgatc ggccagcggc tcagcaggcc gatcccgtag ggatcctggc
     2881 gcggccgggt cgcgcgatgc gggtacaggt caccggtcac cgacaacacg tccgcccatt
     2941 cggccgtcac ctcctgcagg acgacgacgt cggccgggtg tgccgccagc catgccaacg
     3001 caggcgtggc gtcgcgactg cggtaccaga ggttgaccgt cgccacgcgc agcgcgggcc
     3061 cctcgcaatc gcgcccgagg cgcggtgggg tgccggcctg ccacagcagc ggcgtcgcct
     3121 gcagcgcgat cgcgagcagc aacagcgtcg tcgaccggcg gccgcggcgt ccggcgacca
     3181 cgaccacgac gagcacggcg gtggcggcgg cgagttgcca gcggaagtgc gcgaacaact
     3241 cgaacggcca tccgagcggc gccagtccca cggcgagcga gacgatgcat agcgccgcca
     3301 ggctccactc gacccagcga tgcgcgacca cgttcgccgt gcgcatggcg ggggacagta
     3361 gaatcccctg cggcagtacg caacgggcga ggcatccaga catgcaagac acgatgggac
     3421 gcctccccct ccgcgcgtgc actgcgtcgg gtgcggcggc ctggctgtgt gccctcgtcg
     3481 cgtgcggggg cggcgacgat cgacaggcgg ccgatccgac ccgtcgactg tcgcccgtgc
     3541 ccgtcgcggc cgactcggtg acagtgtcgg gcttctcggc cggcggttac atggccgtgc
     3601 agttccacgt ggcccactcg tcgctcgttc aaggcgcggg cgtgttcgcc gcggggccgt
     3661 attactgcgc ggagaattca ctcagagacg cactcggccg ctgcatgcag ggcgacgacg
     3721 gaattccggc cgatcggctg gcgagcctga cgagccagct ggcgctcgac ggggaaatcg
     3781 acccgatcgc ggggctcgcc agcgaccgcg tgtacctctg gcacggcggc gccgacggcg
     3841 tggtcgcaaa gtcggtcgtc gatgcggcgc aggcgtacta cgcgacgctg gtgacacccg
     3901 cctcgatcgc gcgcgtcgaa ctcgaacgcg cggcccacac gtttcccgcg gacgacgcgt
     3961 cgctgcccgc ctgcgacgtg accgcagaac cgttcgtggg gcagtgcgga tacgccgggg
     4021 cccggaacat gctcgagcac ctgtacgacc cgcttgcagc gggcgcatcg ccggttcgac
     4081 cgggcgaact tcgagtcgtc gaccaggccc cgtatgcgaa agcgtccggc gccgacggga
     4141 tggccgcgcg aggctggctc tacgtgcccg cggcctgtgc gggccggggt ccgccgggac
     4201 gctgccgcct gcacgtcgtc ttccacggct gcaagcaggg ggcttcgtac gtggacgaca
     4261 cgttcgtgcg acgttcggga tacctcgcgg cggccgacgc gggcaacgtc gtcgtgctgt
     4321 ttccacaggc ggaacccagc ttccagcccc tgaacccgaa tggctgctgg gactggtggg
     4381 gttacggcag cgaggcctac gcgacgcggc gcggcccgca ggtcatggcg gtcaaggcga
     4441 tggtcgacga cctgctcggg gccgccgcgg cggaaacacg ggcggagtga tcagtacgcg
     4501 gaagacgcgc gcgcgcgcag ggtgcagtac gccacctcga gccggcgcac gcccttgatg
     4561 cgacgcgagg tgcgacggac ctcgcgcacg aggcgcagcg gcaggttcat cgattcgttg
     4621 aggccaccgg cgcagtaatc ttcggcgacg ccgaggtgca gcacggtgac gtgctcgagg
     4681 tcggcacgca tgtacacggc aacgccgatc ggacgctggc tcgcgcgctc gatcgcgaac
     4741 aggcactgca cgtcgggcag cgacgccacg cgcactcgca ggccctcgcc gtcgtcgtag
     4801 atctccggcg gtccgtagcg gtcgatcacg tcgacgatgt cgcgtgcaac gcgctcctgg
     4861 cagccgttga agaacagcag ccgctcgaga gcatggcgcc tgggtcgcgg cacgcgcgac
     4921 gcgaactcga tcgctcccat tagtctcccc gtgggccgct tcggcggcat aggaaggtct
     4981 ggcgatcatg gcggctcgac cggattatgt cgagcggaga cttcctacat cgttgcggca
     5041 gcgcaacaga gcgagccggt cgctctggcg cccggtgaaa cataaaagtt tgaattggct
     5101 cgtattttca gcacgcgaac gtggagtccg cgccgcccat cgcgtgcaat gccgggatat
     5161 gtcagccacc gtcaagctca ggcagctgac cgtcgcgtcg cccgccgaac cggagaccgc
     5221 cgcgccgggc gacccggcgt ccggcagcga cccgcggggc cgcgagggcg tgctcgtcga
     5281 cgtggtcgtc ctgactgccg acatgacgct cttccagtcg gccagggagg ccgtcggcga
     5341 gcgcaatccg gtctggcgcg cccgcagcgc cgaagaagcc gcggacctgc tgatcacggg
     5401 ccgctgcggc gtgctgctga tcgacatggc ctcggtctcc gcgcacggcc agacgctgat
     5461 cggccagatc gtcgcgcagt ttcccgaagt cgtggtctgc gtcgccggca cgcgcgagga
     5521 cgagccttcg ctcgttccgc tgatcaccga aggcctggtc taccggttca tgcacaagcc
     5581 gaccagcgcg cggcgcgcgg gcatgttcct gcaggcggcg atcaagcgcc atctcgagca
     5641 gcgcgagggt cgcggcggcg acgacccgct cgcgctgtgg cgttccgtga cgcgccacgg
     5701 cggcggactg cctcgaaagt acttcgtcgc gttcgcgctg gtgtgcgtgg gattgctcgc
     5761 gacgctgttc ttcggcggca actcacccga tgcaccgccg tcgccgcaaa gtgcgccgct
     5821 gccggatccg atcccggccg tggctacgca ccgcgccgat ccggtgctgt cgcgtgcacg
     5881 cgcggccatg caggccgggc gcctcgaagc cccgcagggc cgcaacgcgc tcgacctgtt
     5941 cgaggccgtg ctgctcgccc agccggatca cgtcgaagcc cgcgccggac tcgacgcgac
     6001 ggttcagcga ctgctggtac gcgcccgcgc agaggccgcc tcgggccgca aggccgaggc
     6061 cgagcggctg ctgcagcgag tgctcgccgt ggtgccctcc cacgcggatg ccaagaccct
     6121 gctggtcgag atcaacccgc cggacttgcc gtctcgacag ctcgagcgcg aacagctggc
     6181 ggaagtccgc gagcgcgcag ccctcgcgcc ggcccccgtc gccctgtcgg gcggcgtccg
     6241 cgcgccggtg gccgtcgcca tcgatccgac gcggccgctg cccgaacccg tgtcgtcagc
     6301 ctacctggcg cggctcgaga gtcgtgcggc gtcgccgcct gtcgcgacgc gcccgaccgt
     6361 cgagtcggat ccgctcgcgc ctcgctacgt gaactcggca ccgccgccgc gcgtcgcaag
     6421 ctggcgtcgt cgtgcaccga cgggcggcgt gccggccgtg gcggcgctgc cgaccgccgg
     6481 actggaacag ccggccgcgc cgggcgtgcc cgagccgcag tccgtcgcac ccgaccccac
     6541 ggtccgcgcc gatgcattcg aacgcgtcca cgcgcccgag cccgtttatc cggcgcaggc
     6601 cctgcgcaac cgcacgcgcg gatgggtcga gctcgaattc accgtcacgc cgaccggcag
     6661 cgtcaccgac gtgcaggttg tgggcgccga gcccgagggc gtcttcgacg cggccgcgac
     6721 acaggcgctg gtacagtggc gcttcaagcc ccgcatcgtg agcggccggg gcgtcgcgca
     6781 gcgcacgagc gtcacgcttc gattcgacgt cgacgactga cctgcagcgt gccgagtcgg
     6841 tacgcgacca gcgcccacgc ggccgcgatg ccggcgccca cccaggcgat tccgaccagg
     6901 cccagcccga acgtggactt cagcagcgcg tgcaccgatg cagacgtcgc gtcaccgccg
     6961 cggtaaacga acgtgtcgat caggctcttg gccttgtatt tctcctcgcg cgtgacgacg
     7021 gtgtagagcg tgtcgcgcga cgggcgcgcg atcgcgtact cgccgacgcg gcgcacgacc
     7081 atgaccccga tcagcaccgc aaagctcggc atcagggcga gcgtcgcgaa tccgagcgtc
     7141 atggccaccg gcaccagcgc gatcagcgcg cggaacccga gccaccggta cagccgcccg
     7201 aacaggaaca gctgcacggt cagcgacgcg gcctgcaccc agaagtcgag cgtgttgaag
     7261 aactcggtgc gcgcgtcgcg gtcgctgaac gtcgcggcca ccatcgcctg ctgctcgagg
     7321 tagaggaagg tcgagaccca cgtcagcagc agcacgaaca ccgcgatcaa ggccaggtac
     7381 ggcgacttcg cgacctgcgc aaacgccgca agcgcgccgc cgccgagtcg cgcgtccggg
     7441 tctgcaccgg ccgccgccgg gtcgcgcacg ccgtggcggc ggtgccaggc gagcaccgcc
     7501 agcatgaagc cgagcccgac cgcgagcagt gccgccgaca ccagcaacag gttgatcgtg
     7561 ccgagcggct gcaccagctg cttgcccagg aacgggccga acaaaccccc caggctgagg
     7621 cccgcggcga tgaagccgaa cagccgcccg gcctgttcgc gcgtgaacac gtccgccatc
     7681 aggctccaga acacggcgac gacgaacagg ttgaagacgc t
//
LOCUS       DASPFR010000044         4893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1112889, whole genome shotgun sequence.
ACCESSION   DASPFR010000044 DASPFR010000000
VERSION     DASPFR010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112889"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VD737_01055"
     CDS             complement(<1..864)
                     /locus_tag="VD737_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXW09177.1"
                     /translation="MEPALSPLDACLVLLTGWIAVGAAGMLRPGHVGFVARALFPLGA
                     LVGLALAIVALSSLALPAERVTLLVGLPDLPFHLRLDPLSRTFLALLGGASAGVSFFA
                     GGYFRKGQGTAPGVLGLQYHLFLASMGFVLLADDAYAFMVAWETMALSSYFLVTSQHG
                     LPEIRSAGFLYLLIAHLGAISILLSFGILQGGTWQFTFDAMRAAELSPAWAAAAFGLA
                     LFGFGAKAGLLPTHVWLPEAHPAAPSPVSALMSGVMLKTAVYGVLRVTFDLLGEPAWW
                     WGLVPVVLGLAT"
     gene            933..1121
                     /locus_tag="VD737_01060"
     CDS             933..1121
                     /locus_tag="VD737_01060"
                     /inference="COORDINATES: protein motif:HMM:NF039406.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HXW09178.1"
                     /translation="MALEDRTARLTILIDPRKKALFERLCAERDTTPSQVVRQMIRRY
                     IEEQTGTPWPEAAGKKRK"
     gene            complement(1143..3059)
                     /locus_tag="VD737_01065"
     CDS             complement(1143..3059)
                     /locus_tag="VD737_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXW09179.1"
                     /translation="MIQLRNLTLSRGVRRLIEDASLQLHAGWRVGVVGANGSGKSSLF
                     ALLRGELHAETGDCAVPRDWRIASVSQETPPLPQPAIEFVLDGDGELRAVERALVRAD
                     EAHDGHALAELHARLAGIDGYSARARAAALMAGLGFAAGDLARPVASFSGGWRMRLNL
                     AQALVSRADLMLLDEPTNHLDLDAVVWLERWLSQYRGTLLLVSHDRDFLDGTVTHVLH
                     LERGRLGLHAGNYSAFEVQKAARLAVQQAMYDKQQREIAHMEEFVTRFRAKASKAQQA
                     QSRLKALDRLERVSAAHVDAPFDFEFPAPARSPDPLLALDDAQLGYGDRPVAGPLTLS
                     LRPGARLGLLGPNGAGKSTLIKTLAGDLDLLAGHRVEGHGLEIGYFAQHQVEQLHGDE
                     SALWHLARVDPRAREQDLRNFLGGFDFGGETADAPVEPFSGGEKSRLALALIVRRRPN
                     LLLLDEPTNHLDLEMRHALTRALAGYEGSLVLVSHDRALLRTVCDGFLLVADGRAVPF
                     DGDLDDYLVWLEQRRAASNVAAAAGVGELAGEREARQARRDAVAADRQQRLLRRRPLV
                     KEVSRLEAKLGALEAERRALESQLADPAWYVATPPPEVQATSRRCAAVVAEITSIEER
                     WLEVQAELEQIGEA"
     gene            3193..4590
                     /locus_tag="VD737_01070"
     CDS             3193..4590
                     /locus_tag="VD737_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HXW09180.1"
                     /translation="MSRIRSVVALCALSLCAGGASAQSSTPAVSSLFAFNISNPTGNL
                     ALGADGAVYGIASPATSTAGGLIYRATVDGSSIDTVVQIEREQAMQPIAGLMLASDGQ
                     FYGTTRFGSIEEASTTGTVYRLSPAGDFAVIHRFATATASNADFNPINTNGAYPEAEL
                     IEGQDGYLYGVASAGGQFGTGAVFKLRRDGSDFQRLHEFAADTDTTTVGLIRTVDGAS
                     PRGKLVQGPDGTLFGTASVGGDNGRGTVFRIAPDGSAFTVLYHFSATTADATTGLLEN
                     TDGAIPVAGLTDGGDGFYYGVTSAGGTEGRGVIFRISPDGATYQVLHQFDGPNGSRPT
                     VELTLGSDGRLYGTTEGGGVNASNATSTLGTIFSIDVAGTNFMRLYSFDGTVGSGPRS
                     SLLELGSADFLGTVGSNGRCGYGSLYRYKGDGTTFEGTDDRCGRRRNNQGGGSGGPAL
                     LLVLGSIAWLRRRAR"
     gene            4616..>4893
                     /locus_tag="VD737_01075"
     CDS             4616..>4893
                     /locus_tag="VD737_01075"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09181.1"
                     /translation="MANTMTHYRTCSLCEAMCGIVVEHRDGEVLSIKPNRDDVLSHGH
                     ICPKAVALKDVHEDPDRLRRPVRRTAEGGWEEITWSAAFDEVARRLGDV"
BASE COUNT          803 a         1716 c         1705 g          669 t
ORIGIN      
        1 cgtggccagg cccagcacca cgggcacgag gccccaccac caggcaggtt cgccgagcag
       61 gtcgaacgtg acgcgcagga caccgtacac ggccgtcttg agcatcacgc cgctcatcag
      121 tgccgacacc ggcgacggtg cggccggatg ggcttcgggc agccagacgt gcgtcggcag
      181 cagcccggct ttcgcgccaa agccgaacag ggccagtccg aacgcggcgg ccgcccaggc
      241 gggcgaaagt tcggccgcgc gcatcgcgtc aaacgtgaac tgccacgtgc cgccctgcag
      301 gatgccgaag ctcagcagga tcgagatcgc gcccagatgg gcgatcagca ggtagaggaa
      361 cccggcgctg cggatctcgg gcaacccgtg ttgcgacgtg accaggaaat acgacgacag
      421 cgccatcgtc tcccacgcca ccatgaatgc atacgcatcg tcggccagca ggacgaaccc
      481 catgctggcg aggaacaggt ggtactgcag gccgagcacg cccggggcgg tgccttgtcc
      541 cttgcggaaa tagccgcccg cgaagaacga gacgccggcc gaggcgccgc cgagcagcgc
      601 caggaacgtg cgggacaggg gatcgaggcg caggtggaag ggcaggtccg gcaggcccac
      661 cagcagcgtg acccgttcgg ccggcagggc gaggctcgag agcgcgacga tcgcgagggc
      721 gaggccgacc agtgcgccga gtggaaacag cgcccgggcg acgaagccga cgtggcccgg
      781 cctgagcatc ccggcggctc cgacggcgat ccaaccggtc agcagcacca ggcaggcgtc
      841 gagcggcgag agcgcgggct ccatgggcgg gattctactc caatgcaaat tgcattataa
      901 tcaaatgcac atcacgatgg agcgtgtgcc gagtggcact tgaagaccgg actgcccgcc
      961 tgaccatcct gatcgacccg cgcaagaaag cgctgttcga gcgcctgtgc gcggagcgcg
     1021 acacgacgcc ctcgcaggtc gtgcggcaga tgatccgtcg ctacatcgag gaacagaccg
     1081 gcactccctg gccggaagct gcgggcaaga aacgcaagta acgcgcgcga gggcgggcgg
     1141 tgtcaggcct cgccgatctg ctccagctcc gcctgcacct cgagccaccg ctcttcgatc
     1201 gacgtgatct cggcgacgac ggcggcgcag cgtcggctcg tcgcctgcac ttcgggcggt
     1261 ggcgtcgcaa cgtaccaggc cggatcggcg agctgcgact ccagggcacg acgctcggcc
     1321 tcgagcgcgc cgagcttcgc ctcgagccgg gacacttcct tgaccagcgg tcgacggcgc
     1381 agcaggcgct gttgacgatc cgccgccacg gcgtcccgtc gtgcctggcg tgcttcgcgc
     1441 tcgccggcga gttcgccgac gcccgccgcg gcggccacgt tcgaggcggc gcggcgctgc
     1501 tcgagccaga cgagataatc gtccaggtcg ccgtcgaagg ggaccgcgcg accgtcggcc
     1561 acgagcagga agccgtcgca gaccgtgcgc agcagcgcgc gatcgtgcga gaccagcacc
     1621 aggctgccct cgtaccccgc gagcgcacgc gtgagcgcgt gacgcatctc caggtccagg
     1681 tgattggtcg gctcgtcgag cagcagcagg ttcggccgtc gccgcacgat cagcgcgagc
     1741 gcgagccgcg acttctcacc gcccgagaac ggctcgaccg gcgcgtcggc cgtctcgccc
     1801 ccgaaatcga agccgccgag aaagttgcgc aggtcctgct cgcgcgcgcg tgggtcgacg
     1861 cgcgcgagat gccacagcgc ggactcgtcg ccgtgcagtt gctcgacctg gtgctgggcg
     1921 aagtagccga tctccagtcc gtgtccctcg acgcgatgac ccgcgagcag gtcgaggtcg
     1981 cccgcgagcg tcttgatcag cgttgacttg ccggccccgt tcggtccgag cagcccgagc
     2041 cgcgcccccg gccgcaggga cagcgtcagc gggccggcga ccgggcgatc cccgtagccc
     2101 agttgcgcat cgtcgagcgc gagcagcggg tccggcgaac gcgccggcgc gggaaactcg
     2161 aaatcgaacg gggcatcgac gtgggcggcc gacacccgct cgagccggtc gagcgccttg
     2221 agccggctct gcgcctgctg cgccttgctc gccttggcgc gaaaccgcgt gacgaattct
     2281 tccatgtgcg cgatctcgcg ctgctgcttg tcgtacatcg cctgttgcac ggccagtcgc
     2341 gcggctttct gcacctcgaa agcgctgtag tttccagcgt gcaggccgag tcgcccgcgc
     2401 tccagatgca ggacgtgcgt cacggtgccg tcgaggaagt cgcgatcgtg ggagacgagc
     2461 agcagcgtgc cgcgatactg cgagagccag cgctcgagcc agacgacggc gtcgaggtcg
     2521 aggtggttgg tcggctcgtc gagcagcatg aggtccgcgc gactgaccag cgcctgcgcg
     2581 aggttgaggc gcatgcgcca gccgcccgaa aaactcgcga cgggacgcgc gaggtcgccg
     2641 gcagcgaaac ccaggccggc catgagggcc gctgcgcgcg cccgggcgct gtagccgtcg
     2701 atgccggcga gccgcgcgtg cagttcggcg agcgcgtgcc cgtcgtgggc ctcgtcggcg
     2761 cgtacgagtg cccgctccac tgcgcgaagt tcgccgtcgc cgtcgagcac gaactcgatc
     2821 gccggctgcg gcaagggcgg ggtttcctgg gacacagacg cgatgcgcca gtcgcgcggc
     2881 accgcgcaat cgcccgtctc ggcgtgcagc tcgccgcgca gcagtgcgaa caggctcgac
     2941 ttgccgctgc cgttggcgcc gaccacgccg acgcgccagc ccgcgtgcag ctgcagcgag
     3001 gcgtcttcga tcagccggcg cacgccgcgg ctcaacgtga ggttacgtag ctggatcaat
     3061 gagtaacggg gctggatcgc ggggggcgaa ttctcccaca gccgggactt tggcgacccg
     3121 tcgcgaactg cggcacaatt cgccgttcgt cagcccgccc ggccccgccg gcgcccggag
     3181 tcaccgatcc cgatgtcccg tatccgttcc gtcgtcgcgc tctgcgcgtt gagcctgtgc
     3241 gccggcggcg cgtctgccca gagcagcacg cccgccgttt ccagcctgtt cgcattcaac
     3301 atctccaacc ccactggcaa cctggcgctg ggagccgatg gggcggtcta cggcatcgca
     3361 tcgccggcca cctcgaccgc ggggggcctg atctaccgcg cgaccgtcga tggatcgtcg
     3421 atcgacactg tggtgcagat cgaacgcgag caggcgatgc agcccatcgc cggcctcatg
     3481 ctcgccagcg acgggcagtt ctacggcacg acccgcttcg gctcgatcga ggaggcctcg
     3541 accaccggca cggtgtaccg gctctccccg gcgggcgact tcgccgtgat ccaccgcttt
     3601 gccacggcca ccgcctcgaa cgccgacttc aacccgatca acaccaacgg ggcctacccc
     3661 gaggctgaac tgatcgaggg gcaggacggt tacctctatg gcgtcgccag cgccggcggc
     3721 cagttcggca ccggtgcggt gttcaagctg cggcgcgacg gatcggactt ccagaggctg
     3781 cacgagtttg ccgccgacac cgacacgacc accgtgggcc tgatcaggac ggtggacgga
     3841 gcctcgccgc gcggcaagct cgtgcagggc ccggacggca cgctgttcgg cacggcctcg
     3901 gtgggcggcg acaacgggcg aggcaccgtg ttccgcatcg caccggacgg cagcgccttc
     3961 acggtgctgt accacttcag cgcgacgacc gccgatgcca cgaccggact gctcgagaac
     4021 accgacggtg ccatcccggt cgccggcctg accgacggcg gcgacggctt ctactacggc
     4081 gtcacgagcg cgggcggcac cgagggccgc ggcgtgatct tcaggatttc gcccgatggt
     4141 gcgacgtacc aggtgctgca ccagttcgac ggcccgaacg gctctcgccc gacggtcgag
     4201 ctcaccctcg gcagcgacgg gcgtctctac ggcacgaccg aaggcggcgg cgtcaatgct
     4261 tcgaacgcga cgtccacgct cggcacgatc ttctcgatcg acgtggcggg cacgaatttc
     4321 atgcgtcttt acagcttcga cggcaccgtc ggcagcgggc cccgcagcag cctcctggaa
     4381 ctgggcagtg ccgatttcct cggcacggtc ggcagcaacg gccgctgcgg ctacggctcg
     4441 ctgtaccggt acaagggcga cggcacgacc ttcgaaggca ccgacgaccg ctgcgggcgc
     4501 cgtcgcaaca accagggcgg cggcagtggc ggcccggcgt tgctgctggt gctgggctcg
     4561 atcgcttggc tgcgacgcag ggcacgctga cgcacacgtc acgggggagg ggtccatggc
     4621 gaacacgatg acgcactacc ggacctgctc cctctgcgag gcgatgtgcg gcatcgtcgt
     4681 cgagcatcgc gacggcgagg tgctctcgat caagcccaac cgcgacgacg tgctgagtca
     4741 cgggcacatc tgcccgaaag ccgtggcctt gaaggacgtg cacgaggacc cggaccgcct
     4801 gcgccggccc gtgcggcgga ccgcggaggg cggctgggaa gaaatcacct ggtcggcggc
     4861 cttcgacgaa gtcgcgcgcc gcctcggcga cgt
//
LOCUS       DASPFR010000045         4548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_747951, whole genome shotgun sequence.
ACCESSION   DASPFR010000045 DASPFR010000000
VERSION     DASPFR010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747951"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /locus_tag="VD737_01080"
     CDS             <1..1511
                     /locus_tag="VD737_01080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HXW09182.1"
                     /translation="LYPQAHVVHLVRDPRAVMASQKNRWRRRKLAANGDAQPRYESVR
                     EWVNYHPYTAGRLWAHATRAALELAEHPRVTLVRYEDLVQHPEQTVRSLCARLGLKFD
                     PAMLDVTQVNSSHHSATGGAHKGMRVDAIDRWRGELTRAEIAITEQIGGPLLRRFGYQ
                     PAAEPRRLRLDRLRFDFSYVVHLAAVLLVNPRRAAIQAKALSRAGSTGAIFRRVRRAD
                     RKRGPVDAGSGGGGVPTAVAAAERDAPPGGVVREFMGLRFWDVPLPRAARFVVDRAAQ
                     GERTQVFFVNAHCINQAASHPGYADLLADAPFLFADGVGMAIAARLFGTRLEHNVNGT
                     DLFPLICEAAAAAAVPVAFLGARPGVARTCADRMQAKYPSLQVVWTDHGYLEPEQEQR
                     RLDDLNASGARILFVAKGVPAQECWIADQRDRLAAPVVLGVGALFDFYSGNVRRAPNM
                     VRDLRSEWLYRLLLEPRRLSRRYLLGNPTFLARTLLWRSMSQPLARRGGSLP"
     gene            1538..2461
                     /locus_tag="VD737_01085"
     CDS             1538..2461
                     /locus_tag="VD737_01085"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HXW09183.1"
                     /translation="MAHPDETSTTGYGERVAFLVGAGRSGTTLLYKLLCLHPGIGFIS
                     NYESRYGWFPDGVAPRAVADCIDAKLHAWFNRSGNAYFVDRPWLTKLFPTPNEGESVY
                     RDCGVPLFPRAGELPDATTSARLRRRFQQMRRRAGAHVFLSKRTANNRRIQYLHHIFP
                     QARYVHLVRDGREVTQSLSTVEWWDAHTVWWDGRTPAEMERSGEPRLAICARNWVREL
                     EELRAQLRLISPDRVIELRYEEILRAPHAQLERVVRFLGLPYTADYRAAISTLQLRPV
                     HARWGEEWSEQQLQCVLRETQPLLRQLGYTD"
     gene            2593..3708
                     /locus_tag="VD737_01090"
     CDS             2593..3708
                     /locus_tag="VD737_01090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXW09184.1"
                     /translation="MTVEAVTPVLHLINGEFYAGAERVQDLLAQRLGRFGYEVEFACL
                     KDGVFDEKRQCKSARLHSMPMRSRVDLSLCYRLARVVRQNRIRLLHTHTPRTALLGAL
                     VARLANVPMVHHVHSPAEADTESRLRNLRNAATERWSLRAARRLIAVSDSLQRYLQER
                     GYAAARIREVPNGVPMAETGRLSYVPGQPLVLGTVALFRPRKGIEVLLEAMARLRAAH
                     VPVRLHAVGPFETPAYEQSVIALTRSLDLQDAVTWAGFRSDVGAEFRRMHVFVLPSLF
                     GEGMPMVVLEAMAAGLPVVSTRVEGIPQVVRDGQDGLLAVPGNAQDLAAALLRFAHGE
                     VNGATLGDSGRQRQRERFSDESMAAGIAQVYREVLRS"
     gene            3752..>4548
                     /locus_tag="VD737_01095"
     CDS             3752..>4548
                     /locus_tag="VD737_01095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXW09185.1"
                     /translation="MGAVIPFRREAPAPRTPSLRVSIVIKALDEESHIRGSLESAIAA
                     AMRVGGEVILADCGSSDRTVEIAREYPVTVVQPRNPDERRRGVGSQLGFERARAEFVY
                     ILDGDMELDPDFLPRALAAMEGDSQLAGIAGVVEEAEGTRPSRALGHRPHERRSQACE
                     WLERGGLYRAEALRDVGYLSNRNLHGLEELELGLRLCAAGWTLRRLGAHGVVHHGRAE
                     GRGDRLRRRWKNRSLDGSGELIRASLGRPYFYRSLQTQRGSLLGLLV"
BASE COUNT          678 a         1537 c         1616 g          717 t
ORIGIN      
        1 acctgtatcc gcaggcccac gtcgtgcatc tcgtccgcga cccgcgcgcc gtcatggctt
       61 cgcagaagaa ccgctggcgg cggcgcaagc tcgcggcgaa tggcgacgcc caaccgcgtt
      121 acgagtcggt gcgggaatgg gtgaactatc atccgtacac ggcggggcgg ttgtgggcgc
      181 acgcaacgcg cgccgcgctg gagctggccg agcacccgcg cgtcacgctc gtgcgctacg
      241 aggatctcgt gcagcatccc gagcagacgg tgcggagcct gtgcgcacgc ctcgggttga
      301 agttcgaccc tgccatgctg gacgtcacgc aggtcaactc gtcgcaccat tccgcgacgg
      361 gcggggcgca caagggcatg cgggtcgatg cgatcgaccg ctggcgcgga gagctcaccc
      421 gtgccgagat tgcgatcacc gaacagatcg gcggtcctct gctgcgtcgt ttcggttatc
      481 aaccggccgc cgaaccgcga cggttgcggc tcgaccgact ccgtttcgat ttcagctacg
      541 tcgtgcatct cgcggccgtg ctgctcgtga atccgcggcg cgccgccatc caggccaagg
      601 ccctctcgcg cgcaggctcc accggggcga tcttccgccg ggtgcgacgc gccgaccgca
      661 agcgcggacc ggtggacgct ggcagcggtg ggggtggcgt tcccacggca gtcgccgctg
      721 ccgagcgcga cgcgccgcct gggggcgtcg tgcgcgagtt catggggctg cgcttctggg
      781 acgtcccgct gccacgggcg gcgcgattcg tcgtggatcg cgcagcccag ggcgagcgta
      841 cgcaggtctt cttcgtcaac gcccactgca tcaaccaggc cgccagccac cctggctacg
      901 ccgacctgct cgccgatgcg ccgttcctct tcgcggacgg tgtcggcatg gccatcgccg
      961 cgcgattgtt cggcactcgc ctcgagcaca acgtcaacgg cacggacctg ttcccgctga
     1021 tctgcgaagc ggctgcggcg gccgcggtgc cggtggcgtt cctcggtgcc cggccaggcg
     1081 tggcacgcac ttgcgccgac cgcatgcagg cgaaataccc gagcctgcag gtcgtgtgga
     1141 ctgaccacgg gtacctcgag cctgagcagg agcagaggcg cctcgacgac ctcaacgcct
     1201 ctggggctcg catcctgttc gtggccaagg gcgtgccggc gcaggaatgc tggatcgcgg
     1261 accagcgcga caggcttgcg gccccggtcg tgctcggcgt cggcgcgctg ttcgacttct
     1321 attccggcaa cgtcaggcgc gccccgaaca tggtccggga cctgcgcagc gagtggttgt
     1381 accgcctgct gctcgagccg cgccggttgt ccaggcgcta cctgctcggc aacccgacgt
     1441 tcctcgccag gacgctgctg tggcggtcga tgagccagcc gctggcgcgc cgcggcggaa
     1501 gcctgccatg agcgccgttg gagaccgagg tcatgacgtg gcacatccgg acgagacgag
     1561 caccaccgga tacggcgagc gcgtggcgtt cctggtcggc gcggggcggt cgggaaccac
     1621 gctgctgtac aagctgttgt gcctgcaccc gggcatcggc ttcatcagca actacgagag
     1681 ccggtacggc tggttcccgg acggggtagc gcctcgcgcc gtcgcggatt gcatcgacgc
     1741 gaagctgcac gcctggttta accggagtgg caacgcgtat ttcgtcgacc gtccgtggct
     1801 cacgaagctc tttccgactc cgaatgaagg cgagtccgtc tatcgcgact gcggtgtccc
     1861 gctgtttccg cgtgccggcg aattgccgga cgcgacaacg agcgcacggc tgcgacggcg
     1921 gttccagcag atgcggcggc gtgcgggcgc gcacgtgttc ctgtcgaagc ggacggccaa
     1981 caatcgtcgc atccagtacc tccaccacat atttccgcag gcccgctacg tccatctcgt
     2041 ccgcgacggt cgcgaggtca cgcagtcgct gtcgacggtc gaatggtggg acgcgcacac
     2101 cgtatggtgg gacggccgca cgcccgccga gatggaacgc tcgggagaac cgcggctcgc
     2161 gatctgcgcc cgcaactggg tccgcgagct ggaggagttg cgcgctcagc tgcgcttgat
     2221 ctcgcccgac cgcgtgatcg agttgcgcta cgaggagatc ctgcgtgcgc cgcacgcaca
     2281 actggagcgc gtcgtgcggt tcctgggcct tccttacacg gccgactatc gtgccgcgat
     2341 ttcgacgctg cagttgcgac ccgtgcacgc gcgctggggc gaggagtgga gcgagcagca
     2401 gctgcagtgt gtgctgcggg agacccagcc gctgttgcgg cagctcggat acaccgattg
     2461 agccgcaccc gcgcacgtcc tgcatgggcg caacgagtca cgacgacgta tgaaccagcg
     2521 cgagtttccc acggggcggc gcacgaacga tcgacgcgat acccaccggt cgcgcggcga
     2581 gagggtgtcg tcgtgaccgt cgaggcagtc acgccggtgc tgcacctgat caacggcgag
     2641 ttctatgccg gggctgagcg cgtacaggac ctgctcgcgc agcgcctcgg ccgcttcggg
     2701 tacgaggtgg agttcgcctg cctcaaggac ggcgtgttcg acgaaaagcg gcagtgcaag
     2761 agcgcccgac tgcacagtat gccgatgcga tcccgtgtcg atctctcctt gtgctaccgg
     2821 cttgcgcgcg tagtgcgaca gaaccgcatc cgtctgctgc acacccacac gccgcgaacc
     2881 gccctgttgg gcgcgctcgt ggcaaggctc gcgaacgtgc cgatggtgca ccatgtgcac
     2941 agcccggccg aagcggacac cgaaagccgg ctgcggaatt tgcgcaacgc cgccacggag
     3001 cggtggagcc tgcgcgctgc gcgacggctc atcgccgtct ccgacagcct gcagcgctac
     3061 ctccaggaac gcggctacgc cgcggcgcgg atccgcgaag taccgaacgg cgtcccgatg
     3121 gcagaaacgg gccggctgtc gtacgtgccc gggcagccgc tcgtgcttgg cacggtggcg
     3181 ctgttcaggc cgcgcaaggg catcgaggtg ctgctcgagg cgatggcgcg gctgcgcgcc
     3241 gcgcacgtgc cggtgcgcct gcacgcggtc gggccgttcg agactcccgc gtacgagcaa
     3301 tccgtcatcg cgctgacgcg gtcgctggac ttgcaggacg ccgtgacctg ggcaggcttc
     3361 cgatccgacg tcggcgccga attccggcgc atgcacgtgt tcgtgctgcc cagtcttttc
     3421 ggggagggca tgccgatggt ggtgctggag gcgatggcag caggcctgcc ggtcgtcagc
     3481 acccgtgtgg agggcatccc gcaggtcgtc cgcgacgggc aggacggctt gctcgcggta
     3541 cccggcaatg cgcaggacct cgccgcggcg ctgctgcggt tcgcgcacgg cgaggtgaac
     3601 ggcgcgacgc tcggcgacag cgggcggcaa cgacagcgcg aacgcttttc cgacgagtcc
     3661 atggcggcag gcatcgccca ggtgtaccga gaggtcctcc gttcgtgatg gcgcacgagc
     3721 gacagcgcga ccagcgtcct ggggcggccg gatgggggcc gtaataccgt ttcgccgcga
     3781 ggcgcccgcg ccgcgcaccc cgtcgttgcg cgtgtcgatc gtgatcaagg cgctcgacga
     3841 ggaaagccac atccgaggct ctctcgaatc ggccatcgcc gcggcgatgc gcgtcggcgg
     3901 ggaagtgatc ctggccgact gtggctcctc ggatcgcacg gtcgagatcg cgcgcgagta
     3961 tcccgtcacc gtggtccagc ccaggaaccc cgacgagcgt cgccgcggcg tcggttcgca
     4021 gctcgggttc gagcgggcgc gcgcggagtt cgtgtacatc ctcgacggcg acatggaact
     4081 cgatccggac ttcctgccgc gggcactcgc cgcgatggaa ggggactcgc agctcgccgg
     4141 cattgcgggc gtggtcgagg aagccgaggg gacacgcccg tcccgagcgc tcggccatcg
     4201 cccgcacgaa cgacgatcac aagcgtgcga atggctggag cggggcggac tctaccgagc
     4261 cgaggccctc cgtgacgtcg gttacctctc gaaccgcaac ctgcacgggc tggaggaact
     4321 ggagctcggc ctgcggcttt gcgccgccgg gtggacgctg cggcggctcg gcgcgcacgg
     4381 cgtggtgcac catggccgcg cggagggcag gggagaccgg ctcaggcgcc gctggaagaa
     4441 tcgctccctg gatgggtcgg gggagctgat ccgtgcgtcg ctcggccggc cgtatttcta
     4501 ccgcagcctg cagacccagc gtggctcgct gctggggctg ctggtctg
//
LOCUS       DASPFR010000046         4751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_870461, whole genome shotgun sequence.
ACCESSION   DASPFR010000046 DASPFR010000000
VERSION     DASPFR010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870461"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VD737_01100"
     CDS             <1..184
                     /locus_tag="VD737_01100"
                     /inference="COORDINATES: protein motif:HMM:NF017024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HXW09186.1"
                     /translation="VRGGDIVGDHRLIFAGPGEQVELAHHAQDRSGFARGALVAARWA
                     AGRPPGLYSMFDVLGI"
     gene            547..1707
                     /gene="carA"
                     /locus_tag="VD737_01105"
     CDS             547..1707
                     /gene="carA"
                     /locus_tag="VD737_01105"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HXW09187.1"
                     /translation="MTTPAVLALEDGTVFRGVSIGALGTTTGEVVFNTAMTGYQEILT
                     DPSYARQIVTLTYPHIGNTGTNPEDLESGIVHAAGLVIRDLPAVHSSWRATESLPEFL
                     RRRNTVAIAEIDTRKLTRILREKGAQAGCIAAGDAAQRPDLAIEAARRFPGLKGMDLA
                     KVVCTEAPYQWNEGTNWSLEQGPKRRAGQRLHVVAYDYGIKRNILRLLADHGCRMTVV
                     PAQTTAAEAMALAPDGIFLSNGPGDPEPCTYAIEATQAFLEQGVPLFGICLGHQLLGL
                     AVGARTVKMKFGHHGANHPVLELETGRVLITSQNHGFAVDETTLPANVKATHRSLFDG
                     SLQGITCTDRPAFSFQGHPEASPGPRDVTPLFAQFIESMLRRGVQAPRAKTV"
     gene            1726..>4751
                     /gene="carB"
                     /locus_tag="VD737_01110"
     CDS             1726..>4751
                     /gene="carB"
                     /locus_tag="VD737_01110"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HXW09188.1"
                     /translation="MPKRTDLESVLIIGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPDMADAIYIEPINWRTVARIIEKERPSALLPTMGGQTALNCALDL
                     VREGVLEKHGVELIAASREAIDMAEDRELFRNAMRDIGLECPRSRVAHSMEEALAIQA
                     EIGFPTVIRPSFTLGGSGGGIAYNREEFEQIVARGLDASPTSEVLLEESVIGWKEFEM
                     EVVRDRADNCIIICSIENLDPMGVHTGDSITVAPAQTLTDKEYQLMRDASIAVLRKIG
                     VECGGSNVQFAISPDDGRMLVIEMNPRVSRSSALASKATGFPIAKVAAKLAIGYTLDE
                     LRNEITGGATPASFEPTIDYVVTKIPRFTFEKFPGANARLTTQMKSVGEVMAMGRTFQ
                     ESLQKALRGLETGIDGLTPIATLPLADDDARNALLEELRMPGPHRLRYVADAFRAGLS
                     MDEVHRACRIDPWFLVQVDDLVREEGRVAEQGLDALDEPRLRRLKRKGFADSRLARLL
                     GTTEADVRNRRHGLGVRPVYKRVDTCAAEFATSTAYLYSTYEDECEAAPTNRRKIVIL
                     GGGPNRIGQGIEFDYCCVHAALALREDGFETIMVNCNPETVSTDYDTSDRLYFEPLTF
                     EDVMEILHVEKPFGVIVQYGGQTPLKLAKALGDAGVPIIGTSPDSIDVAEDRERFQQL
                     VERLQLRQPPNRTARTEDEAVRLATEIGFPLVVRPSYVLGGRAMEVVFTEDDLRTYMT
                     HAVQVSNDSPVLLDRFLDLAIEVDVDAICDGKDVYVGGIMEHIEQAGVHSGDSGCSLP
                     PNSLSPALQEQIREQTRRLALALSVIGLMNVQFAIQNGVVYVLEVNPRASRTAPFVSK
                     ATGVPMAKAGARVMAGKTLAELGLTRERVPKFFSVKEAVFPFNKFPDADPILGPEMKS
                     TGEVMGTGRSFGEAYAKAQAASGVTLPTRGVCLISVRERDKPAAVELGKRLVRQGFEI
                     VATHGTADWLQRAGIDCRRANKVREGRPHIVDMIKN"
BASE COUNT          802 a         1635 c         1620 g          694 t
ORIGIN      
        1 cgtgcgcggt ggcgacatcg tcggcgacca ccggctgatc ttcgcggggc cgggcgagca
       61 ggtcgaactg gcccatcacg cgcaggaccg ctcgggtttc gcccggggcg ccctcgtcgc
      121 cgcgcgctgg gccgcgggcc gtccgccggg cctgtactcg atgttcgacg tgctcgggat
      181 ctgacgcctc gaatcgcccg cggcatggat gccgcggccc cggaaattgg ggacactcct
      241 gatttccgta tggacccgac ccgcctgaga cgcgcccgga tcggaaattc aggagtgtcc
      301 ctattttccg tacggcgtcc cggcgatccg acctccggaa cggccggcag ccgcagccat
      361 gcgatgcgcg ggctcgctcg caccgaactg gaacggtcgg cgcggacgcc cgcgtagcgt
      421 acgccgaaac gctcgcgtag aattctccac ttagcccgtc cgacaaaagt cctccggagt
      481 gagcaacgct cagcttcggc ggtcgtcgca cgcgcgaata tatcgatcgc tgcgccggag
      541 gtacccgtga ccacacccgc tgtcttggcc ttggaggacg gcaccgtgtt tcggggcgtt
      601 tcgatcggcg ccctcggcac gaccaccggc gaggtggtgt tcaacaccgc gatgacgggc
      661 taccaggaga tcctgaccga tccgtcgtat gcacggcaga tcgtcacgct cacctatccg
      721 cacatcggca acacgggcac caatccggaa gacctcgagt cgggcatcgt ccacgccgcc
      781 ggcctcgtga tccgtgacct gccggcggtg cactccagct ggcgggcgac ggagtcgctg
      841 ccggaattcc tgaggcgtcg caataccgtc gcgatcgccg agatcgacac ccgcaagctc
      901 acgcggatcc tgcgcgagaa aggtgcacag gccggctgca tcgccgcggg cgacgccgcc
      961 cagcgcccgg atctcgccat cgaggccgct cgccggttcc ccgggctcaa gggcatggat
     1021 ctcgccaagg tcgtctgcac cgaggcaccc taccagtgga acgagggcac caactggtcg
     1081 ctggagcagg gcccgaagcg tcgggcgggg cagcggctgc acgtcgtcgc gtacgactac
     1141 ggcatcaagc gcaacatcct gcggttgctg gcggaccacg gctgccgcat gaccgtcgtg
     1201 ccggcgcaga cgacggcggc cgaggcgatg gcgctcgccc cggacggcat cttcctctcc
     1261 aacggcccgg gcgacccgga gccgtgcacg tacgcgatcg aggccaccca ggcgttcctg
     1321 gagcagggag tccccctgtt cggcatctgc ctgggccacc agttgctcgg cctcgcggtc
     1381 ggcgcccgca cggtcaagat gaagttcggc catcacggcg ccaatcaccc ggtgctcgaa
     1441 ctcgagaccg gccgggtgct gatcacctcg cagaaccacg gcttcgcggt cgacgagacg
     1501 acgctgcccg ccaacgtcaa ggcgacgcac cgctcgctgt tcgacggttc gctgcagggc
     1561 atcacctgca cggaccgccc cgccttcagc ttccaggggc accccgaggc gagccccggg
     1621 ccgcgcgacg tgacgccgct gtttgcccag ttcatcgagt cgatgctgcg ccggggcgtg
     1681 caggcgccca gagccaagac ggtctgaagc aggagtcgat cgcgaatgcc caagcgcacg
     1741 gacctcgaga gcgtcctgat catcggagcc gggccgatcg tgatcggcca ggcctgcgag
     1801 ttcgactatt cgggcgcgca agcctgcaag gcgctgcgtg aggaaggcta ccgcgtcatc
     1861 ctggtcaact cgaatcccgc gacgatcatg accgacccgg acatggccga cgcgatctac
     1921 atcgagccga tcaactggcg caccgtcgcc cgcatcatcg agaaggagcg cccgagcgcg
     1981 ctgctgccga ccatgggtgg ccagacggcg ctcaactgcg cgctcgacct cgtgcgcgag
     2041 ggcgtgctcg agaagcacgg cgtcgagctg atcgccgcct cgcgcgaagc gatcgacatg
     2101 gccgaggacc gcgagctgtt ccgcaacgcg atgcgcgaca tcgggctcga gtgcccgcgt
     2161 tcgcgcgtgg cgcacagcat ggaggaagcg ctcgcgatcc aggccgagat cgggttcccc
     2221 accgtgatcc ggccgtcgtt cacgctcggc ggctccggcg gcggcatcgc ctacaaccgc
     2281 gaggagttcg agcagatcgt cgcgcgcggc ctcgacgcgt cgccgaccag cgaggtgctg
     2341 ctcgaggaat cggtgatcgg ctggaaggaa ttcgagatgg aggtcgtccg cgaccgggcg
     2401 gacaactgca tcatcatctg ctcgatcgag aacctcgacc cgatgggcgt gcacacgggc
     2461 gactcgatca ccgtcgcgcc cgcgcagacg ctgaccgaca aggagtacca gctgatgcgc
     2521 gacgcctcga tcgcggtact gcgcaagatc ggcgtcgagt gcggcgggtc caacgtgcag
     2581 ttcgcgatca gccccgacga cgggcggatg ctggtcatcg agatgaatcc gcgcgtgtcg
     2641 cgctcgtcag cgctcgcgtc gaaggcgacc ggcttcccga tcgccaaggt cgccgcaaag
     2701 ctcgccatcg gctacacgct cgacgagctg cgcaacgaga tcaccggcgg cgcgacgccg
     2761 gcgtcgttcg agccgacgat cgactacgtc gtcaccaaga tcccgcgctt cacgttcgag
     2821 aaattcccgg gcgccaacgc acggctcacg acgcagatga agtcggtcgg cgaggtcatg
     2881 gccatgggcc gcaccttcca ggagtcgctg cagaaggcgc tgcgcggcct cgagaccggc
     2941 atcgacggac tcacgccgat tgcgacgctg ccgctcgccg acgacgacgc gcgcaacgcg
     3001 ctgctcgagg aactacgcat gccgggaccg catcggttgc gctacgtcgc cgacgccttc
     3061 cgcgccgggt tgtcgatgga cgaggtgcat cgcgcgtgcc gcatcgaccc gtggttcctg
     3121 gtgcaggtcg acgatctcgt gcgcgaggaa ggcagggtgg ccgagcaggg cctcgatgcg
     3181 ctcgacgagc cgcggttgcg tcggctcaag cgcaagggct ttgcggacag ccggctggcc
     3241 aggctgctcg gcacgaccga ggcggacgtg cgaaaccggc ggcacgggct cggcgtgcgg
     3301 ccggtctaca agcgcgtcga cacctgcgcc gccgagttcg cgacctcgac ggcctatctc
     3361 tattcgacct atgaggacga atgcgaggcc gcgccgacga accggcgcaa gatcgtgatc
     3421 ctcggcggcg gcccgaaccg gatcgggcag ggcatcgagt tcgattactg ctgcgtgcac
     3481 gcggcactcg cgctgcgcga ggacgggttc gagaccatca tggtcaactg caacccggag
     3541 acggtctcga ccgactacga cacctcggat cgactgtact tcgaaccgct cacgttcgag
     3601 gacgtgatgg agatcctgca cgtcgagaag ccgttcggcg tgatcgtgca gtacggcggg
     3661 cagacgccgc tcaagctcgc caaggcgctg ggcgacgccg gcgtccccat catcggcacg
     3721 agccccgact cgatcgacgt cgccgaggat cgcgagcggt tccagcaact cgtcgagcgg
     3781 ctgcagctca ggcagccgcc caaccggacc gcccgcaccg aggacgaagc ggttcgcctg
     3841 gcgacggaga tcggcttccc gctggtcgtg cggccgagct acgtgctcgg cggaagggcc
     3901 atggaggtcg tcttcaccga ggacgacctg cgcacgtaca tgacgcacgc cgtgcaggtg
     3961 tcgaacgaca gcccggtgct gctcgatcgc ttcctcgacc tcgcgatcga agtcgacgtc
     4021 gacgcgatct gcgacggcaa ggacgtctac gtcggcggga tcatggagca catcgagcag
     4081 gcgggcgtgc actcgggtga ttcgggctgc tcgctgccgc ccaacagcct gagcccggcc
     4141 ctgcaggagc agattcgcga gcagaccaga cgcctggcgc tcgcgctcag cgtgatcggc
     4201 ctgatgaacg tgcagttcgc gatccagaac ggcgtggtct acgtgctcga ggtcaacccg
     4261 cgcgcctccc gcacggcgcc gttcgtctcc aaggcgacgg gcgtgccgat ggcgaaggcg
     4321 ggggcccggg tgatggcggg caagaccttg gccgagctcg ggctcacccg ggagcgggtg
     4381 ccgaagttct tctcggtcaa ggaggcggtg tttccgttca acaaattccc ggacgccgac
     4441 ccgatcctcg gtcccgagat gaagtcgacc ggggaagtca tggggacggg tcgcagcttc
     4501 ggcgaggcct atgccaaggc ccaggccgcc tcgggtgtca ccctgccgac gcgcggcgtc
     4561 tgcctgatca gcgtgcgcga gcgcgacaag ccggccgcgg tcgagctcgg caagcggctc
     4621 gtccggcagg gcttcgagat cgtggcgacg cacggcacgg ccgactggct gcagcgggcg
     4681 ggcatcgact gccgccgcgc caacaaggtc cgcgagggcc ggccccacat cgtcgacatg
     4741 atcaagaacg a
//
LOCUS       DASPFR010000047         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_260876, whole genome shotgun sequence.
ACCESSION   DASPFR010000047 DASPFR010000000
VERSION     DASPFR010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260876"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="VD737_01115"
     CDS             <1..1357
                     /locus_tag="VD737_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HXW09189.1"
                     /translation="RRRLGLRDVVERAWHALGGPATLGSERELDEALAYLDALGEVEA
                     QARGSGVDLATLAEALQKLYAPARPRADTRVELLTIHKSKGLQFDTVIVPGLERVGRT
                     DAPPLLRWLKLPGRDGHRLLIAPITAAGADERDPLHAWLGRIEDEKLLQEKRRLLYVA
                     ATRAERELHLLGSCAVTTDEATRAHAVRAPDQSSALGLLWGVPEVRAAFDARLGEVDA
                     IAGDPALQVPRNPIVVRVPDAWATPPPPDGPPIDVHARVPSVTATDLEFDWATETARH
                     VGTVVHRELQRLAREGCGLPEGAARQQLLARYECELAELGVPRDRRRAATGRVLDALQ
                     RTVRDTRGRWLLDTPHRDAQSELALTGRVGRDVVSIVIDRTFVDDAGVRWIVDYKTSS
                     HEGAGLDVFLDNERERYRAQLERYAGLARALGDEPIRLGLYFPLLSAWREWGAEAVFS
                     V"
     gene            complement(1445..>2431)
                     /locus_tag="VD737_01120"
     CDS             complement(1445..>2431)
                     /locus_tag="VD737_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXW09190.1"
                     /translation="RRLLQGAALLMCATGLGFAGFSSLAPLLLIAFLGTLNPSSGDVS
                     VFLPLEHARLAEASAQGRDRVTLFARYSLTAAVCSAVGALGAAIPDLLASRGLERLTA
                     FRWMFAFYALAGLVVWALYRRLPQPSALPTVTKTSGLGPSRGTVVRLASLFAVDAFAG
                     GLVVQSLLALWLFEKFELSLSAAGTFFFWAGLLTAGSQLAAPWVAHRIGLLNTMVYTH
                     VPSSLALIAAAFAPRVEVALGLLLVRAALSQMDVPTRSAFVMSMVTPPERPAAASFTA
                     VPRSLASAISPAIGGALFAAGWLAAPLVACGALKIAYDLAILAAFRRVAVRS"
BASE COUNT          413 a          857 c          874 g          287 t
ORIGIN      
        1 gcgtcgacgt ctcggactgc gcgacgtcgt cgaacgcgcc tggcatgcgc tcggcgggcc
       61 cgccacgctc ggcagcgagc gcgaactcga cgaggcgctc gcgtacctcg atgcgctggg
      121 ggaggtcgaa gcgcaggccc gcggctccgg cgtcgacctc gccacgctgg ccgaggcgct
      181 gcagaagctg tacgcccccg cgcgcccccg cgccgacacc cgcgtggaac tgctgaccat
      241 ccacaagtcg aagggcctgc agttcgacac ggtgatcgtg cccgggctcg agcgcgtcgg
      301 ccggaccgat gcgccaccgc tgctgcgctg gctcaagctg ccggggcgcg acggtcaccg
      361 gttgctgatc gcgccgatca cggcggcggg tgcggacgag cgcgatcccc tgcacgcgtg
      421 gctcggacgg atcgaggacg aaaagctgct gcaggagaag cggcgcctgc tgtacgtggc
      481 cgccacgcgc gccgagcgcg agttgcacct gctgggctcg tgcgcggtca cgacggacga
      541 ggccacgcgg gcgcacgccg tccgcgcacc cgaccagtcg agcgcactcg gcctgctgtg
      601 gggcgtaccc gaggttcgcg ccgccttcga tgcgcgactc ggcgaggtcg acgcgatcgc
      661 gggcgatccg gcgctgcagg tcccgcggaa cccgatcgtc gtgcgcgtgc cggacgcatg
      721 ggccactccg cccccgcccg acggcccgcc gatcgacgtg catgcgcgcg tacccagcgt
      781 gacggcgacg gacctcgagt tcgactgggc caccgagacc gcgcgacacg tcggcaccgt
      841 cgtccaccgg gaactgcagc ggctggcgcg ggaggggtgt ggactgccgg aaggcgcggc
      901 ccggcagcaa ttgctggcgc gatacgagtg cgaactcgcc gaactcggcg tccccaggga
      961 ccgccgccgc gccgccacag ggcgcgtgct cgacgcattg cagcgcacgg tgcgggacac
     1021 gcgcggtcgc tggttgctcg acacgccgca tcgcgacgcg cagtcggaac tcgcgctgac
     1081 gggccgggtc ggccgcgacg tcgtcagcat cgtcatcgat cgcacgttcg tcgacgacgc
     1141 gggcgtgcgc tggatcgtcg actacaagac gagttcgcac gaaggcgcag ggctcgacgt
     1201 gttcctcgac aacgaacgcg aacgctaccg ggcgcagctg gaacgctacg ccgggctggc
     1261 gcgggcgctc ggcgacgaac cgatccggct gggcctctac tttccgctgc tgtcggcgtg
     1321 gcgggagtgg ggggcggagg cagtgttcag tgtctagtgg ctagtgtcta gtaagaacga
     1381 accccctcga tgccgcgatg cctactaacc gctagacact agacacgaga cacttctcca
     1441 ccggctacga acgaaccgcg acgcggcgaa acgcagcaag gatcgccagg tcgtaggcga
     1501 tcttcagggc accgcaggcg accaggggag cggcgagcca gcccgccgcg aacaacgcgc
     1561 cgccgatcgc gggactgatc gccgacgcga ggctccgtgg cacggccgtg aagctggcgg
     1621 ccgcagggcg ctcgggcggg gtgaccatgg acatcacgaa cgccgatcgc gtcgggacgt
     1681 ccatctgcga cagcgcagcc cggaccagga gcagcccgag cgcgacctct acgcgtggcg
     1741 cgaaggccgc ggcgatcagg gcgaggctcg acggcacgtg cgtgtacacc atcgtgttga
     1801 gcagcccgat ccggtgggcc acccacggtg ccgcgagctg cgagcccgcc gtcagcaatc
     1861 ccgcccagaa aaagaacgta ccggcggctg acagcgagag ctcgaatttc tcgaacagcc
     1921 agagcgcgag cagcgactgc accacgaggc caccggcgaa agcgtccacg gcgaacaggc
     1981 tcgccagtcg caccacggtg ccgcgcgacg gcccgagccc ggatgtcttc gtgacggtgg
     2041 gcagcgcgga cggttgcggc agccgccggt acagcgccca caccaccagt ccggccaagg
     2101 cgtagaacgc aaacatccac cggaacgcgg tcagccgctc gaggccgcgt gaggcgagca
     2161 ggtctggaat cgcggccccc agcgcgccta ccgccgaaca gacggccgcc gtgaggctgt
     2221 agcgggcgaa cagcgtcacc cggtcgcgtc cctgtgccga cgcctccgcg agacgggcgt
     2281 gctccagcgg caggaagacg ctcacgtcac ccgaactcgg gttcaaggtg cccaggaaag
     2341 cgatcagcag caggggcgcg agggacgaga atcccgcgaa gccgagaccc gtcgcacaca
     2401 tgagcagggc ggcgccctgc agcagccgtc g
//
LOCUS       DASPFR010000048         3254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_382923, whole genome shotgun sequence.
ACCESSION   DASPFR010000048 DASPFR010000000
VERSION     DASPFR010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382923"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            529..1515
                     /gene="ilvC"
                     /locus_tag="VD737_01125"
     CDS             529..1515
                     /gene="ilvC"
                     /locus_tag="VD737_01125"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010158896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HXW09191.1"
                     /translation="MRAYSKKDVDKKALRGARIAVVGYGSQGRAHALNLKDSGYDVVI
                     GVRKGDSWKQAKKDGLAVAVPAEAVKGATLVALLTPDTTQAENYKEILPNLAQGATLL
                     FAHGFNIHFGQIKPRKDLDVVLIAPKGPGGLVRRQYQEGRGVPCLIAIAQDASGKAKA
                     KALAYADGIGGTRAGVIETTFKEETETDLFGEQAVLCGGATELVLKGYETLVEAGYQP
                     EVAYYECLHELKLIVDLLHEGGLAKMHKYISHTAKWGDLTRGPRVVNSGTKKEMKKIL
                     KEIQDGQFARVWIRENKTGRKKYDALLKKDLAHPIEKVGAGLRARMPWLNQT"
     gene            1559..3124
                     /locus_tag="VD737_01130"
     CDS             1559..3124
                     /locus_tag="VD737_01130"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HXW09192.1"
                     /translation="MAQRDPNRVLIFDTTLRDGEQSPGCSMTQPEKLRMAKALAELGV
                     DVIEAGFPIASRGDWEAVHEIAREVRGPIIAGLARCNREDIDKAWSAVRVAARPRIHV
                     FLATSAIHREHKLGMAKDEIIHSAVEGVKYARSLCEDIEFSPEDASRTELEFLREVVE
                     AVIAAGATTINIPDTVGYTVPEEFYEVFSYLKQNVRGAEDVTFSVHCHNDLGLAVANS
                     LAAIRGGARQVECTINGIGERAGNASLEELTMALKVREGIYGLHTGIDGKRLFPTSRL
                     LSSITGMPIPRNKAIVGENAFAHESGIHQHGMLKHHSTYEIMRPHDVGMSRTNLVLGK
                     HSGRAALRERIKELGFELSDVELTRVFNDFKTLADKKKELFDGDIEALVLKHEVETAG
                     PWTLENLHVVSDSSKFGTGRVVLRHADGRIVSQESSGDGPVDAALKAIEQATGVFAKL
                     RKFEVRSVTVGEDAQGEAVLTCDYNGRSYRGTSVTTDIVESSARAFVEIVNAIETSRR
                     RTADAGEVPAAAV"
BASE COUNT          626 a         1071 c         1091 g          466 t
ORIGIN      
        1 ccagccgtcg aagtcgcgct gcgtgacgag ccagagcgaa agggtgctcg catcgtccag
       61 ttccgacgcc tcgatcagca ggtccacgtg aaaccccggt gagtgtctag tgttcagtgt
      121 ctagcggtta gtcggaggta cgccgtcctc ggcgcgggca gctgcctctt accagacact
      181 agacactaaa cactagacac ttccgttttg cgttgcggtt gacaatccgt cggccgctgt
      241 gctgctatgc accatctctt acttgctgtc agctgcaggc gccggatgaa gtcgtcgttc
      301 gactcgtcgc ttcccctcga cgtcgcggta caccccgcgc tggttggcct gctgcttgcg
      361 tcgcttaccc tgcttcccct gctgcgaagg agcggggctc cgggctgaga gcgcagaagg
      421 caaggcgcga ttggacacca ggaaccccgc acggcgagac cgccggagcg gggttccgac
      481 gttttggacg accgcaattt cagtgaccag cttctggaga ggacaacgat gcgcgcgtat
      541 tcgaagaagg acgtcgacaa gaaggccctg aggggagcca ggatcgccgt cgtcggttat
      601 ggcagccagg gtcgcgcgca cgcgttgaac ctgaaggact ccggctacga cgtcgtgatc
      661 ggggtccgca agggcgacag ctggaagcag gcgaagaagg atggcctcgc cgtcgccgtg
      721 cccgccgagg cggtcaaggg cgccacgctc gtcgcgctgc tcacgccgga caccacccag
      781 gcggagaact acaaggaaat cctgcccaac ctcgcgcagg gcgccacgct gctgttcgcg
      841 cacggcttca acatccactt cggccagatc aagccgcgca aggacctgga cgtggtgctg
      901 atcgcgccca agggccccgg tggcctcgtg cgccggcaat accaggaagg tcgcggggtg
      961 ccgtgcctga tcgcgatcgc gcaggacgcg tcgggcaagg ccaaggcgaa ggcgctcgcc
     1021 tatgcggacg gcatcggcgg cacgcgcgcc ggcgtcatcg aaaccacgtt caaggaagaa
     1081 accgaaaccg acctgttcgg cgagcaggcg gtgctctgcg gcggcgcgac agagctcgtg
     1141 ctcaagggct acgagacgct ggtcgaggcc ggctaccagc ccgaggtcgc ctattacgag
     1201 tgcctgcacg agctgaagct gatcgtcgac ctgctgcacg aaggcggcct cgccaagatg
     1261 cacaagtaca tctcgcacac ggccaagtgg ggtgacttga cgcgggggcc ccgggtcgtg
     1321 aactccggca ccaagaaaga aatgaaaaag atcctcaagg agatccagga cggccagttc
     1381 gcacgcgtct ggatccgcga aaacaagacc ggtcgcaaga agtacgacgc gttgctgaag
     1441 aaggatcttg cgcacccgat cgagaaggtg ggtgcaggcc tgcgcgcccg catgccgtgg
     1501 ctcaaccaga cctgaggcga cgcccttccc tgccccctct gcacgcacgg tgacgaacat
     1561 ggcccagcga gacccgaacc gcgtactgat cttcgatacg acgctgcgcg acggcgagca
     1621 gtcgcccggc tgcagcatga cgcagcccga gaagctgcgg atggcgaagg cgctcgccga
     1681 actgggagtc gacgtcatcg aggcgggctt cccgatcgcc tcgcgcggcg actgggaagc
     1741 cgtgcacgag atcgcgaggg aggtgcgtgg cccgatcatc gcgggcctcg cccgctgcaa
     1801 ccgcgaggac atcgacaagg cctggagcgc ggtgcgcgtc gcggcgcgcc cgcgcatcca
     1861 cgtcttcctc gccacgagcg cgatccaccg cgagcacaag ctcggcatgg cgaaggacga
     1921 gatcatccac agcgccgtcg agggcgtgaa gtacgcgcga agcctctgcg aggacatcga
     1981 gttctcgccc gaggacgcct cgcggaccga gctcgagttc ctgcgcgagg tcgtcgaagc
     2041 cgtcatcgcg gccggcgcca cgacgatcaa catccccgac acggtcggct acaccgtgcc
     2101 cgaggagttc tacgaggtct tctcttacct gaagcagaac gtgcgcggcg ccgaggacgt
     2161 gaccttcagc gtgcactgcc acaacgacct ggggctcgcc gtggccaaca gcctggcggc
     2221 gatccgtggc ggcgcgcgcc aggtcgagtg cacgatcaac ggcatcggcg agcgggccgg
     2281 caacgcgtcg ctggaagagc tgacgatggc gctcaaggtg cgcgaaggca tctacggcct
     2341 gcacaccggc atcgacggca agcgcctgtt cccgaccagc cgcctgctct cgagcatcac
     2401 cggcatgccg atcccgcgca acaaggcgat cgtcggcgag aacgcgttcg cccacgaatc
     2461 gggcatccac cagcacggca tgctcaagca ccactcgacg tacgagatca tgcggccgca
     2521 cgacgtcggc atgtcgcgca ccaacctggt gctgggcaag cacagcggtc gcgctgcgct
     2581 gcgcgagcgc atcaaggaac tcggcttcga gctctccgac gtcgagctca cgcgcgtctt
     2641 caacgacttc aagaccctgg ccgacaagaa gaaggagctg ttcgacggcg acatcgaggc
     2701 actggtgctc aagcacgagg tcgagacggc cgggccctgg acactcgaga acctgcacgt
     2761 cgtgagcgac tcgtcgaagt tcggcaccgg ccgcgtcgtg ctgcgtcacg ccgacggccg
     2821 catcgtgtca caggagtcgt cgggcgacgg tccggtcgac gcggcactga aggcgatcga
     2881 gcaggcaacc ggggtattcg cgaagctgcg caagttcgag gtgcgcagcg tgacggtcgg
     2941 cgaggacgcc cagggcgagg ccgtcctgac gtgcgactac aacggccgca gctaccgcgg
     3001 cacgagcgtg acgacggaca tcgtcgagtc gagcgcgcgc gccttcgtcg aaatcgtcaa
     3061 cgccatcgag acctcgcgcc gccgcacggc cgacgcgggc gaagtgccgg ccgcggccgt
     3121 atgaccgagg acggctcggc ggtgtccgtg cgcttgccag acatggacgt caagcagcgg
     3181 cactgcgaag agagagcgac cgtcccggcc cctcccggag tgcgacggaa gaccgtcacg
     3241 agccatggat ggcg
//
LOCUS       DASPFR010000049         2777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_626902, whole genome shotgun sequence.
ACCESSION   DASPFR010000049 DASPFR010000000
VERSION     DASPFR010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626902"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VD737_01135"
     CDS             <1..218
                     /locus_tag="VD737_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09193.1"
                     /translation="GVLCLVLVRARRTSNLARACFEQLTCQISALALLRGLFAPQRNP
                     TDPIDFVTLMDAQGAGDVQTLARPSTE"
     gene            complement(282..1304)
                     /gene="bmt"
                     /locus_tag="VD737_01140"
     CDS             complement(282..1304)
                     /gene="bmt"
                     /locus_tag="VD737_01140"
                     /EC_number="2.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF005718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine--homocysteine S-methyltransferase"
                     /protein_id="HXW09194.1"
                     /translation="MNALSRLLASGRPLLADGATGTNLFAMGLTSGDAPERWNLEHPD
                     RVRELHRRFVEAGADVVLTNTFGANARRLALHGLDTRVREVNRVAAQLARSVADAAGR
                     TVVVGGSVGPTGELFEPLGALTESAAVEVFAEQIAGLAEGGADVAWIETMSALEEIRA
                     AVLAAAQVGLPCTFTASFDTAGRTMMGVTPQALVAFAQSLEPRPLAIGANCGVGAADL
                     VMSVLGLTAHDAGIPVIAKANAGIPRVSGAHVHYSGTPDLMARYVELAADAGARIIGG
                     CCGTGFDHLAAMRAALDAHGGGERPDEARVVEMLGPLQAPAARIGEAASPREGRRSRR
                     PGPPVG"
     gene            complement(1339..1959)
                     /locus_tag="VD737_01145"
     CDS             complement(1339..1959)
                     /locus_tag="VD737_01145"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXW09195.1"
                     /translation="MRLHTSPRAPNPRRVDMFLAEKNIDGLERRTYDLNRGDLRTPEF
                     RALNPLGRVPVLELDDGRCLAESRAICSYLETLHPEPNLMGRDAVERAFIESADRQVE
                     LFLLFSIANAVRHTHPGLAPLEQPQFADFGNSQAGKLLEYAAAFDRLLASRPWLAGDR
                     FTIADITGFCGVEFAKLIKFRPAEAGFPHLADWRERVAARPSAKLA"
     gene            complement(1982..2692)
                     /locus_tag="VD737_01150"
     CDS             complement(1982..2692)
                     /locus_tag="VD737_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HXW09196.1"
                     /translation="MIELFTAATPNGYKASIALEELALPYTMRVLDLAQGEQKQAWYR
                     AINPNARIPAIVDHDADDFAVFESGAILIYLAEKTGRLLPADAKGRSRVLQWLMFQMG
                     GIGPMMGQANVFYRYLPEKIPLAIDRYQGEVKRLFRVLDERLGASEYLAGDYSIADIA
                     NWAWVRTWKWSGVPIDDLPHLARWVEQIRARPAVQAALQKPPSRANLAEDDAQARKFI
                     EEARKMVVTGVDRTAEQP"
BASE COUNT          397 a          948 c          975 g          457 t
ORIGIN      
        1 caggcgtgct ttgcctcgtg ctggtgcggg cgcgcagaac gtcgaatctc gcccgtgcgt
       61 gtttcgagca gttgacgtgc cagatctctg cgctcgccct gctgcgtggc ctgttcgcgc
      121 cacagcgcaa tccgacggat cccatcgatt tcgtaacgct catggacgca cagggtgcgg
      181 gcgacgtgca gacattggcc cggccgtcga cggagtaggc cgacgcccat cgccccgagg
      241 atcgtgccgg cgcgccccgc ggtcgctgtc cctgggaaag ttcagccgac gggtggtccc
      301 ggtcttcgcg aacgtcgccc ctcgcgcggg gaggctgcct caccgatccg cgccgcaggt
      361 gcctgcagcg gccccagcat ctcgacgacg cgggcttcgt ccggccgctc gccgccgccg
      421 tgggcgtcca gcgccgcgcg catcgcggcc aggtgatcga agccagtgcc gcaacacccg
      481 cctatgatcc gcgcgccggc atcggccgcc agttccacgt agcgggccat caggtcgggc
      541 gtgccggagt aatgcacgtg ggcgccgctg acgcgaggaa tcccggcgtt ggctttggcg
      601 atgaccggga tgcccgcgtc gtgagccgtg aggccgagca cgctcatgac caggtcggcg
      661 gcgccgacgc cgcagttggc gccgatcgcc agcgggcgcg gttcgagcga ctgcgcaaac
      721 gcgacgagtg cctgcggcgt cacgcccatc atcgtacgcc cggccgtgtc gaagctggcc
      781 gtgaaagtgc agggcagccc cacctgcgcc gcggcgagca ccgccgcgcg gatttcctcg
      841 agcgcggaca tcgtttcgat ccacgcgacg tcggcaccgc cttcggccag cccggcaatc
      901 tgctcggcga agacttcgac cgctgcgctt tcggtcaagg cgccgagcgg ctcgaagagt
      961 tcgccggtcg gcccgaccga tccgccgacg acgacggtgc gcccggcggc gtccgcgacc
     1021 gaacgtgcca gctgcgcggc gacccgattg acttcgcgta cgcgcgtgtc caggccgtgc
     1081 agggccagcc ggcgcgcatt ggcaccgaac gtgttcgtca gcaccacgtc ggcgccggcc
     1141 tcgacgaagc ggcggtgcag ctcgcgcacg cggtcggggt gctcgaggtt ccagcgctcg
     1201 ggcgcatctc cggaggtgag gcccatcgcg aacaggttcg tgcccgtggc gccgtcggcc
     1261 agcagcgggc gacctgaggc gagcagccgc gagagcgcgt tcacgtgccg tgcccgtcgt
     1321 tcgaaggaga cgacgccgtc acgcgagttt cgcgctcgga cgtgcggcga cacgctcacg
     1381 ccagtcggcg agatgcggga aaccggcctc cgccggacgg aacttgatca gcttggcgaa
     1441 ctcgactccg cagaaccccg tgatgtcggc gatcgtgaat cggtcccctg ccagccacgg
     1501 gcgtgacgcc agcaggcggt cgaacgcggc cgcgtactcg agcagcttgc cggcctgcga
     1561 gttgccgaag tccgcaaatt gcggctgctc gagcggcgcg agccccgggt gcgtgtgtcg
     1621 gacggcgttc gcaatcgaaa acagcaggaa caactcgacc tgccggtccg cgctctcgat
     1681 gaacgcgcgc tcgaccgcat cgcgtcccat caggttcggt tcgggatgca gggtttccag
     1741 gtagctgcag atcgcccgcg actcggcgag gcagcggccg tcgtcgagtt ccaggacggg
     1801 gacgcgtccc agcgggttca gcgcgcggaa ctccggcgtg cgcaggtcgc cacggttgag
     1861 gtcgtacgtg cgtcgctcga gcccgtcgat gttcttctcg gcgaggaaca tgtcgacacg
     1921 tcgtggattc ggtgcgcgcg gcgaggtgtg cagtctcatg tcttcggtcc gccgtagtgg
     1981 atcagggctg ttcggccgtg cggtcgacgc cggtgacgac catcttgcgg gcttcctcga
     2041 tgaacttgcg cgcctgcgcg tcgtcctcgg ccaggtttgc tctcgagggt ggcttctgca
     2101 gtgcggcctg cacggcgggc cgtgcgcgga tctgctcgac ccagcgtgcc aggtgcggaa
     2161 ggtcgtcgat cggcacgccc gaccatttcc atgtgcgtac ccacgcccag ttggcgatgt
     2221 cggcgatcga gtaatcgccc gcgaggtact cgctcgcgcc gagccgctcg tcgagcacgc
     2281 ggaacaggcg cttgacctcg ccctggtagc gatcgatcgc aagcgggatc ttctccggca
     2341 ggtatctgta gaagacgttg gcctggccca tcatcgggcc gatgccgccc atctggaaca
     2401 tcagccactg cagcacgcgc gaacggccct tcgcatcggc cggcaggagg cgtccggtct
     2461 tctccgccag gtagatcagg atcgcgcccg actcgaagac cgcgaagtcg tccgcgtcgt
     2521 ggtcgacgat cgcggggatg cgggcgttcg ggttgatcgc gcggtaccac gcctgcttct
     2581 gctcgccctg cgcgaggtcc agcacgcgca tggtgtacgg cagggcgagt tcctcgagcg
     2641 caatggatgc cttgtagccg ttcggcgtgg ccgccgtgaa gagttcgatc atcggtgcct
     2701 gccggacgct cgggggacgg tcgagcttag ccgagtcagt tgttctttgc gccctgctcg
     2761 acccagcgcc ggatcgt
//
LOCUS       DASPFR010000050         7268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_627143, whole genome shotgun sequence.
ACCESSION   DASPFR010000050 DASPFR010000000
VERSION     DASPFR010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627143"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..1822
                     /locus_tag="VD737_01155"
     CDS             254..1822
                     /locus_tag="VD737_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXW09197.1"
                     /translation="MRADPLLLPVRVSPWLLTLLLVGMMPLHAADDAQKASDSAATKP
                     ETTEPAPASSVTRHQIQLDGKALKYTATVGWYILKDAEDKPIARFGYTAYTLDGIKDL
                     ARRPVTFAFNGGPGSSSIWLHMGIMGPRRAVVNDPGYAAPPPAQIVDNAYTPLDVTDV
                     VMIDPVGTGFSKPLGDAKPADFHGVDEDIRSVAQFIKRYVSENGRWGSPKYVLGESYG
                     GMRGAGLAYHLQAEQGMNLNGLVVVSPFLNAETGIDGAGIDYAHVAYLPTLAATAWYH
                     GLVQDKPASIAEYMTDVERFAREEYLPALARGYVLPEAQKQAVAAKLARYTGTTPQYW
                     ERADLRVTHTQFLQELKRSDRLVAGRIDSRYIGPSVNPLAGEMDYDPFFPAIGPAYTS
                     AFLSYLHGELQFGRDEEYRVSAFDIEWSWEHEQPGNAGWMSPIPNTVPDLALALTMNP
                     GLHVLVQQGWYDLATPYLATKMDLEHLDVAPEARDRIHVKYYDAGHMMYVHGPSMQQF
                     RDDLVGFIRETDRL"
     gene            complement(1889..2293)
                     /locus_tag="VD737_01160"
     CDS             complement(1889..2293)
                     /locus_tag="VD737_01160"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXW09198.1"
                     /translation="MARGECNCGAVQFEIDADLKDVYVCHCSICRRFTGSQGIAVVVF
                     PRQQFRWLQGEDHVATWQKPGTDWHAYFCRACGSAVPGENDPHTMFAPAGSITQGGEA
                     LEVRHHVWVGSKAPWDRIGDDGRQHAEGFRAG"
     gene            complement(2359..3045)
                     /locus_tag="VD737_01165"
     CDS             complement(2359..3045)
                     /locus_tag="VD737_01165"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HXW09199.1"
                     /translation="MNPWQRVRLARARRASRLPLQQRLLEQALLPAAGARVDDLELVA
                     LDFETTGLDVQRDHVVAAGWVRIVRGRIVLASARELHVRTARPEGVGQSAAIHGILDS
                     DLDSAVEPRALVEALLPDLHGRVIVAHAAAIERGFLARLLRDLGGVPVPNVFLDTMTI
                     EQRLLQAGGVQVRERQADLSLDACRRRRGLPAHAQHSAASDALACAELLLAQLAEFGA
                     AGRVRLADLR"
     gene            complement(3042..4892)
                     /locus_tag="VD737_01170"
     CDS             complement(3042..4892)
                     /locus_tag="VD737_01170"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HXW09200.1"
                     /translation="MNSALADVEKFLAATPPFDLLDAGLLTRAASAIEIFYRRRGEVL
                     LDIGDLNATLYLVRRGAVEAHDATGRLVDRYGEGESFGVLSVLTGKPVRLRITAIEDC
                     LVWSLPRAALDELRVRSTEFETFFIRSVEERLTAAIQPRASAGQTLFMTPLGELVRRE
                     LVCVEPSTPITAAARAMSQHGVSSLLVAEGEQVRGIVTDRDLRNRVLAQGRDPAGPVA
                     TIMTPDPLTLDAARPVLEGILAMVSRGIHHLPLTRDGRIFGVVTTRDLMTLQTRHPLY
                     LAAQVQKAGSVAELVLTCRGIPRLFELLVASGMRAEQVPKVLTTLSDAVTRRLLDLAE
                     QHLGPPPAPYAWLAFGSQARGEQSLRTDQDNGLVYADGAPAEAAPYFADLATLVCDGL
                     DACGYPYCPGNVMATNDSWRRPVAGWLAHFRDWAALPDPEAVLRASIFFDLRRVHGDP
                     ALGAQLTEAVAATGSGLGKSVFLTALARQAARYDVPLGFFRRFVLESRGQHRETLDIK
                     AAGLMPLTDLVRVRALQAGIAVPGTQSRITALVELGRLSRADGERLAGVHDLLAGLRV
                     RLHAEQVRRGEPPHNHLDPASLGHAERTALKDAFLIIREAQQGLQMDFAT"
     gene            4962..5705
                     /locus_tag="VD737_01175"
     CDS             4962..5705
                     /locus_tag="VD737_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09201.1"
                     /translation="MPQDDKPRWFRRVVRREPPRHATGSLPWSAAELWSALRSHLGLK
                     REALEGPIDTAEALGRFLDERASFLAQTSLYGYLQTRAGMRYPELFDDEAFVVSINVA
                     KWQLWLDCLSDLAVYTGSRIAQQRPDETPRIGRAMELVVDQVLDRTGTPQEAGADFAA
                     HAQRVRHRVARTDWLAVGAGEAAFTESPAGIVRWAPVKDELKVLDEEIVRNSVRFRWQ
                     AVRRDFAEQLRLQPLVQSLTAQSTSSRPR"
     gene            complement(5678..6034)
                     /locus_tag="VD737_01180"
     CDS             complement(5678..6034)
                     /locus_tag="VD737_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09202.1"
                     /translation="MIEGIPPWAVALDYLLGVVMWTLIARSAMRLFLPEESSFFFNRF
                     FVRATQPLLDRFRPITPAFLVPALVPLYVAWFFFMFRFYVMPALLGYSVMGMLSFPLE
                     GQVARGIYHLGRLLVD"
     gene            complement(6101..>7268)
                     /locus_tag="VD737_01185"
     CDS             complement(6101..>7268)
                     /locus_tag="VD737_01185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HXW09203.1"
                     /translation="IAYLIPIFWMSIVQGFGFLPHFQYGEAVRRIMELEPIIGVGTAA
                     VDSVEGLKVLTALHATPPEGALAAWKFVTLVACMMVGTASLPHILMRYFTTPSVRDAR
                     NSVAWSLLFIFLLYFSAPALATFSKLQMIDPTLPTSIIGASIDQVMSLEWIQKWSAIG
                     LLAIRDSNADGLLQINEFFLNPDIIVLATPEMAGLPYVISGLVAAGGMAAAMSTADGL
                     LLAMANALSHDLYYKIIDPKAEVKRRLMTSRVLLLIIGAGGAAVASLKLTGILGAVAW
                     AFCFAMSGLFVPLVLGVWWKRANRPGALAGMIVGLGTGWWYLLAVRNPDAWYGIAKPW
                     IGLDDIRFGVVGVTASAIAMIVVTLLTPAPSEETQRMVDDVRVPTGDTIIPSKA"
BASE COUNT         1171 a         2537 c         2554 g         1006 t
ORIGIN      
        1 cggcgagcgc gagccggcca ggatggcctc ttgctggcgt gtctgcccga cccgtccgga
       61 ccgggcacgc ccggacgacg cgctcctcgt gacgctcccc gcctcgaaga tccggcggcg
      121 cccccgccgg agttggttcc cgctcatacg tgttcgaagc ggcagctccc gctgtagagc
      181 attctgccgc ggggttgcat cacgcaccgt ttcgcctgag catcgcggcc ttcgtgcctg
      241 ccggagaccg cgcatgcgtg ctgacccgct ccttctgcct gtccgtgttt cgccgtggct
      301 gctgacgctg ctgctggtcg gcatgatgcc gctgcacgcg gcggacgacg cccagaaggc
      361 cagtgacagc gcggcgacca agccggagac caccgagccg gctcccgcct cgtcggtgac
      421 caggcaccag atccagctcg acggcaaggc gctcaagtac accgcgacgg tgggttggta
      481 catcctcaag gacgcggagg acaagccgat cgcgcggttc ggctacacgg cgtacacgct
      541 cgacggcatc aaggacctgg cgcgacggcc ggtcacgttc gcgttcaacg gcggcccggg
      601 ctcgtcgtcg atctggctgc acatgggcat catgggcccg cgccgcgccg tcgtgaacga
      661 cccgggctac gcggcgccgc ccccggcgca gatcgtcgac aacgcgtaca cgccgctcga
      721 cgtgaccgac gtggtgatga tcgacccggt cgggaccggg ttcagcaagc cgctcggcga
      781 cgccaagccc gcggacttcc acggcgtcga cgaggacatc cgctcggtcg cccagttcat
      841 caagcggtac gtctccgaga acgggcgctg gggttcgccc aagtacgtgc tgggcgagag
      901 ctacggcggc atgcgcggtg cgggcctcgc ttatcacctg caggccgagc agggcatgaa
      961 cctgaacggg ctcgtggtcg tctcgccatt cctcaacgca gagaccggca tcgacggcgc
     1021 gggcatcgac tacgcgcacg tcgcctacct gccgaccctc gcggccaccg cgtggtacca
     1081 cggcctggtg caggacaagc ccgcgtcgat cgcggagtac atgacggacg tcgaacgctt
     1141 cgcccgcgag gaatacctgc cggcactggc caggggctac gtgctgccag aggcgcagaa
     1201 gcaggcggtc gcggcgaaac tcgcccgcta caccggcacg acgccccagt actgggagcg
     1261 cgcggacctg cgcgtgaccc acacccaatt cctgcaggag ctcaagcgca gcgaccgcct
     1321 ggtcgccgga cgcatcgact cgcgttacat cggcccgtcg gtcaacccgc tcgcgggcga
     1381 gatggactac gacccgttct tcccggccat cggaccggcg tacacctcgg cgttcctgag
     1441 ctacctgcac ggcgagctgc agttcggtcg cgacgaggag taccgcgtct ccgcgttcga
     1501 catcgagtgg agctgggagc acgagcagcc tggcaacgcg ggctggatgt cgccgatccc
     1561 gaacaccgtg ccggacctcg cgctcgcgct gacgatgaac ccggggttgc acgtgctggt
     1621 gcagcagggc tggtacgacc tggcgacgcc gtacctcgcg acgaaaatgg acctcgagca
     1681 cctcgacgtc gcgcccgagg cacgcgaccg catccacgtg aagtactacg acgccgggca
     1741 catgatgtac gtgcacgggc cgtcgatgca gcagttccgc gacgacctgg tcggcttcat
     1801 ccgggagacc gatcgcctgt aggccacggc cacgcgcggg ctgctgcgac gacgcggagg
     1861 tgaatcgtcg gtcgggccgg gcggcgcttc agccggcgcg aaaaccctcc gcgtgctgcc
     1921 gaccgtcgtc gccgatccgg tcccacggcg ccttcgagcc cacccacacg tggtgtcgca
     1981 cctccagtgc ctcgccgccc tgcgtgatcg agcccgccgg cgcgaacatc gtgtgtggat
     2041 cgttctcgcc cgggaccgcc gacccgcacg cgcgacagaa gtaggcgtgc cagtcggtgc
     2101 ccggcttctg ccaggtcgct acgtggtctt cgccctgcaa ccagcggaac tgctgcctcg
     2161 ggaacacgac gacggcgatg ccctggctgc cggtgaagcg ccggcagatc gagcagtggc
     2221 acacgtagac gtccttcagg tcggcgtcga tctcgaactg caccgcgccg cagttgcatt
     2281 cgcccctggc catgcgtagc cctcctggtg tcagcgatgg tagcgcggcc agcgcggcct
     2341 gggccggcgc ggtgctcctc agcgcaggtc cgcgagccgc acgcggccgg cggcgccgaa
     2401 ctccgcgagt tgcgcgagca gcagttccgc gcaggcgagc gcgtccgacg ccgcggaatg
     2461 ctgcgcgtgc gccggcaggc cgcgacgccg ccgacaggcg tcgagcgaga ggtcggcctg
     2521 gcgttcgcgc acctgcacgc cgccggcctg gagcaggcgc tgctcgatcg tcatcgtgtc
     2581 gaggaagacg ttgggcacgg gcaccccgcc gaggtcgcgc agcaatcgag cgaggaaccc
     2641 gcgttcgatc gcggctgcgt gcgcgacgat gacccgcccg tggaggtccg gcagcagcgc
     2701 ctcgaccagc gcgcgcggct cgaccgcgga gtcgaggtcg ctgtccagga ttccgtggat
     2761 cgcggcgctc tggccgacgc cttcggggcg ggcggtccgc acgtgcagtt cccgggcgct
     2821 cgcgagcacg atccggccac gcacgatgcg cacccagccg gccgccacga cgtggtcgcg
     2881 ttgcacgtcg agaccggtcg tctcgaaatc gagcgcgacg agttccaggt cgtcgacgcg
     2941 ggcgccggcc gcaggcagca acgcctgctc gagcagtcgc tgttgcagcg gcagccggct
     3001 ggcccggcgc gcacgggcga gccgcacacg ctgccaggga ttcacgtcgc gaagtccatc
     3061 tgcagtccct gctgcgcctc gcggatgatc aggaacgcat ccttgagcgc cgtgcgctcg
     3121 gcgtgtccga gcgatgccgg gtcgaggtgg ttgtgcggag gctcgccgcg tcgcacctgc
     3181 tcggcatgca ggcgcacccg cagcccggcc agcaggtcgt ggacacccgc gagccgctcg
     3241 ccatccgcgc ggctcagccg gcccaactcg acgagtgcag tgatgcgcga ctgcgtgccg
     3301 ggcaccgcga tgccggcctg cagggcgcga acgcggacca gatcggtcag cggcatcagg
     3361 ccggccgcct tgatgtcgag cgtctcgcga tgctggccgc ggctctcgag cacgaagcgc
     3421 cggaagaacc cgagtggaac gtcgtagcgc gccgcctgtc gcgcaagggc cgtcaggaac
     3481 acgctcttgc cgaggccgct gccggtcgcc gcgaccgcct cagtcagctg cgcgccgagc
     3541 gcgggatcgc cgtgcacgcg acgcaggtcg aagaagatgc tcgcgcgcag cacggcctcc
     3601 gggtcgggca gcgcggccca gtcccggaaa tgggcgagcc agccggcgac cggccggcgc
     3661 cacgagtcgt tggtcgccat cacgttgccg gggcagtatg gatagccaca ggcgtcgagg
     3721 ccgtcgcaga ccagggtcgc gaggtccgcg aaatacggcg cggcttcggc cggcgcgccg
     3781 tcggcgtaga cgagcccgtt gtcctggtcg gtgcgcaggc tctgctcgcc gcgcgcctgg
     3841 gagccgaacg caagccaggc atagggcgcc ggaggtggac cgaggtgctg ctccgcgagg
     3901 tccagcaggc ggcgcgtgac cgcgtcgctg agcgtcgtca gcaccttcgg cacctgctcc
     3961 gctcgcatgc ccgatgcgac cagcagctcg aacagacgcg ggatgccacg gcacgtcagc
     4021 acgagctcgg cgacggagcc ggccttctgc acctgcgcgg cgaggtagag cgggtgccgc
     4081 gtctgcagcg tcatcaggtc gcgcgtcgtg acgacgccga agatgcgccc gtcgcgcgtg
     4141 agcggcaggt ggtggatgcc acggctcacc atcgcgagga taccctcgag cacgggacgc
     4201 gcggcgtcga gcgtcagcgg atccggcgtc atgatcgtcg cgacgggccc ggccgggtcg
     4261 cggccctgcg cgagcacgcg attgcgcagg tcccgatcgg tcacgatgcc gcggacctgc
     4321 tcgccctcgg ccaccagcag cgaggacacg ccgtgctgcg acatcgcgcg tgccgcggcc
     4381 gtgatcggcg tcgacggctc cacgcagacc agctcccggc gcacgagctc gccgagcggc
     4441 gtcatgaaga gggtctgtcc ggccgaggcg cgcggctgga tcgcggccgt caggcgttcc
     4501 tcgacgctgc ggatgaagaa ggtctcgaac tccgtcgaac gcacccgcag ctcgtcgagc
     4561 gccgcacgcg gcaggctcca caccaggcag tcctcgatcg cggtaatccg cagccgtacg
     4621 ggcttgccgg tcagcaccga gagcacgccg aagctttcgc cttcaccgta ccggtcgacg
     4681 aggcgcccgg tggcgtcgtg cgcctccacg gcgccgcgac gcaccaggta cagcgtggcg
     4741 ttgaggtcgc cgatgtcgag cagcacctcg ccccggcggc ggtagaaaat ctcgatggcg
     4801 gaggccgcgc gcgtgagcag gccggcgtcg agcaggtcga acggcggcgt ggcggcgagg
     4861 aacttctcga cgtcggccag ggcgctgttc atggcgcatc atagcccggc gcgcgcggcc
     4921 tatactggcg ccgttgcgag ccgcgagtgc cggattcgac cgtgccccag gacgacaagc
     4981 cgcgatggtt tcggagggtc gtgcggcgcg agccgccgcg gcacgcgacc ggttctttgc
     5041 cgtggagcgc tgccgaactc tggtcggccc tccgctcgca cctgggcctc aagcgcgagg
     5101 cactcgaggg accgatcgat accgccgagg cgctcgggcg gttcctcgac gagcgcgcga
     5161 gcttccttgc ccagacctcg ctctacggat acctgcagac gcgcgcaggc atgcgctatc
     5221 cggagctctt cgacgacgaa gcgttcgtcg tctcgatcaa cgtcgccaag tggcagctct
     5281 ggctcgactg cctgtccgac ctcgcggtct acacgggcag ccgcatcgcg caacagcggc
     5341 ccgacgagac gccgcggatc ggccgcgcaa tggaactggt cgtcgaccag gtgctcgatc
     5401 gcacgggtac gccgcaggag gccggcgcgg acttcgccgc gcacgcgcag cgcgtacgcc
     5461 accgcgtggc gcgcaccgac tggctcgcgg tgggagccgg cgaagcggcg ttcaccgaaa
     5521 gtccggccgg catcgtgcgg tgggccccgg tcaaggacga gctcaaggtg ctggacgaag
     5581 agatcgtccg caattcagtg cgcttccgct ggcaggccgt gcgacgggat tttgccgagc
     5641 agctgcggct gcagcccctg gtccagtcgt tgacggctca gtcgaccagc agccggccga
     5701 ggtgatagat gccgcgcgcg acctggccct cgagcggaaa agagagcatg cccatgaccg
     5761 aatagccgag cagcgccggc atcacgtaga agcggaacat gaagaagaac caagccacgt
     5821 agagcggcac cagcgccggc accaggaacg cgggcgtgat cgggcgaaac cggtcgagca
     5881 gcggctgcgt ggcgcgcacg aagaaccggt tgaagaagaa cgagctctcc tccggcagga
     5941 acagccgcat cgcggagcgg gcgatcagtg tccacatcac gacgccgagc aggtagtcca
     6001 gcgcgaccgc ccagggcggg atcccctcga tcaaggctcg ctcctcaatg aaacgccggg
     6061 gcgagtcgcc tcgccccggc gcaatgacgc aggcctgccg tcaggccttg gacgggatga
     6121 tcgtgtcgcc ggtcgggacg cgcacgtcgt cgaccatgcg ctgcgtctcc tcgctcggtg
     6181 ccggcgtcag cagcgtgacg acgatcatgg cgatcgcgct cgccgtcacg cccaccacgc
     6241 cgaaccggat gtcgtcgagg ccgatccacg gcttggcgat gccgtaccag gcgtcgggat
     6301 tgcgcaccgc cagcaggtac caccagccgg tgccgaggcc gacgatcatg ccggcgagcg
     6361 cgccgggccg gttggcacgc ttccaccaga ccccgagtac cagcggcacg aagagccctg
     6421 acatcgcgaa gcagaacgcc caggcgacgg cgccgaggat gccggtgagc ttgaggctcg
     6481 cgaccgcggc gccgcccgcg ccgatgatca gcagcagcac gcgcgaggtc atcagccggc
     6541 gcttaacctc ggccttcggg tcgatgatct tgtagtacag atcgtgcgac agggcgttgg
     6601 ccatcgcgag caggagcccg tcggcggtcg acatcgcggc cgccatgccg ccggcggcga
     6661 ccaggccgga gatcacgtac ggcagcccag ccatttccgg cgtggccagc acgatgatgt
     6721 ccgggttcag gaagaactcg ttgatctgca gcaggccgtc ggcattgctg tcgcggatgg
     6781 caagcaggcc gatcgccgac cacttctgga tccattcgag cgacatcacc tggtcgatcg
     6841 aggcgccgat gatggaggtc ggcagggtcg gatcgatcat ctgcagcttg ctgaacgtcg
     6901 caagagccgg cgccgagaag tagagcagga agatgaacag cagcgaccac gccacggagt
     6961 tgcgcgcgtc gcgcaccgag ggcgtggtga agtagcgcat caggatatgc ggcagcgagg
     7021 ccgtgccgac catcatgcag gcgaccaggg tcacgaactt ccatgccgcg agcgcgccct
     7081 cgggcggtgt ggcgtgcagg gcggtcaata ctttcaggcc ctccacgctg tcgaccgccg
     7141 ccgtgcctac gccgatgatc ggctcgagtt ccatgatgcg ccgcacggct tcgccgtact
     7201 ggaagtgcgg caggaagccg aacccctgca cgatcgacat ccagaagatg gggatcaggt
     7261 aggcgatg
//
LOCUS       DASPFR010000051        11401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_872048, whole genome shotgun sequence.
ACCESSION   DASPFR010000051 DASPFR010000000
VERSION     DASPFR010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11401
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872048"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            379..1140
                     /locus_tag="VD737_01190"
     CDS             379..1140
                     /locus_tag="VD737_01190"
                     /inference="COORDINATES: protein motif:HMM:NF022665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3014 domain-containing protein"
                     /protein_id="HXW09204.1"
                     /translation="MSAATDLVPPSARDLLQTEDMVRRWVITVDALPRARLPQRARPV
                     QRVPDQLVAGGAPDAPTLSPDNYARYEPYVALAESIDPQALVSAYRWLYPLFQQAYTE
                     VVRPEGYFNDRLIEVIDHLLATPELPAEPQLSRPSVTYRLADPDLEALSAGRKALLRM
                     GPDNARRVKAVLARLRAELSADSPARAGQGVQDDGCSRGRTKKAAGPCPPPSFVDASA
                     EVTGSVRCAARTRSDPRCSSWPSCRARGAGRCSRS"
     gene            complement(1037..1684)
                     /locus_tag="VD737_01195"
     CDS             complement(1037..1684)
                     /locus_tag="VD737_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXW09205.1"
                     /translation="MLNKIPVVIIDDHHVICAGLRSIIDSQPDMRVVAEALTGRKGLT
                     AIQEHQPKLVLLDLQLGGDMSGLDVLRSLQTHQSTTRVLMLSAYSEAQYGLNVLRAGA
                     AGFIAKGVEPAELLRAMRMVIRGGRYLGPALAEQLVASASGEDPQGPLHSRLSAREFQ
                     IFCKLAQGESVSQVAAKMFLSVKTVSTYRRRVLDKMAMKSNADLTAYALRNELIQ"
     gene            1876..2298
                     /locus_tag="VD737_01200"
     CDS             1876..2298
                     /locus_tag="VD737_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXW09206.1"
                     /translation="MQLGWLSLAITATVAYHLVLKLTPAGANPFLTLMLTYAVVTLAF
                     GVGLALGPGPFEWRQELRALNWTAVALAVAIVALDLGFLFLYRSGYDVSLGALVTQST
                     AALLLLVIGVLVFRERLSVANGVGLALCLAGLWLVNRR"
     gene            complement(2309..2500)
                     /locus_tag="VD737_01205"
     CDS             complement(2309..2500)
                     /locus_tag="VD737_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09207.1"
                     /translation="MRIARPILLVTTPIGVIFGLREAWRLAGPKMALLMAAMLAVVTA
                     LVWWTVRTIRAEEKDEESG"
     gene            complement(2506..2820)
                     /locus_tag="VD737_01210"
     CDS             complement(2506..2820)
                     /locus_tag="VD737_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09208.1"
                     /translation="MPTGLRTSSRARRLLDHHRLRGRAYPTMRYWERLYQQLSEEAEK
                     RGKTPPPPPMWHGLDQDPSEQDRMDRLDEQVVWADRNNLLHRVQMFFDAMPPSGWKMR
                     EP"
     gene            2933..3307
                     /locus_tag="VD737_01215"
     CDS             2933..3307
                     /locus_tag="VD737_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HXW09209.1"
                     /translation="MTIAYWCVLIAGLLPYVSTLAAKIGGERFDNRNPRDWLASQSGF
                     RGRANSAQLNGFEAFPLFAAGVVIAQLAGAAPSRVDELAMAFVAARVLYTLFYFADMA
                     LLRSLAWSVGIGCSAALFFIAA"
     gene            3327..3992
                     /locus_tag="VD737_01220"
     CDS             3327..3992
                     /locus_tag="VD737_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09210.1"
                     /translation="MDAEPDSLERLRSFREEYRRRHVPRGYRGGRHLFFTFGLGSAAL
                     LACLLQLEQVQPLEWATVPLTALYSNLAEYFGHRGPMHHRRPGLGLVFERHARQHHRF
                     FTAAAMPVDTLQDLRAVLFPPVLMTFFLAAFALPVAWLLAVAVSANVAWLFGATSLAY
                     FLNYEFLHLAYHLPPRYAVARWPLVGRLKRLHETHHDPRLMTHYNFNISYPLGDWLFR
                     TLR"
     gene            complement(3996..4922)
                     /gene="rarD"
                     /locus_tag="VD737_01225"
     CDS             complement(3996..4922)
                     /gene="rarD"
                     /locus_tag="VD737_01225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HXW09211.1"
                     /translation="MRRADVPPNGPDPSSASSGAGLAAAIAAFTIWGAMPLYLRPLQA
                     QSALEIMAHRIVWCCLLVFAWLALRGELHAVRAALANRGVRLRLVASATLISLNWLVY
                     VWAVNHDHVIDSSLGYFINPLFSVVLGVMVLGERLNRPQQFAVGLAVVGVVYLAVVAG
                     RPPWIALVLAASFGLYGLIRKVVAVDAVPGLATETLLLTPFALAWLLWLEWRGLGALG
                     RAAPHVNAMLVGSGLITALPLALFAYGARRIPLSTVGLVQYIGPTLQFLTGVWIFHEP
                     FSRERGIGFLFIWAALAIYAVDGLWRNRRWRA"
     gene            5044..5436
                     /locus_tag="VD737_01230"
     CDS             5044..5436
                     /locus_tag="VD737_01230"
                     /inference="COORDINATES: protein motif:HMM:NF023184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HXW09212.1"
                     /translation="MDNRLDQGRLAREWRTMQAMVAVYCHGHGHVAAAGCKLCADCAR
                     FLDYAGRRLEKCPYGESKPTCAKCPIHCYKPEPRALARQVMRYSGPRMAWRHPWLSLT
                     HLTDKLRRVEHPMTARRRERRVTAARGE"
     gene            complement(5448..5639)
                     /locus_tag="VD737_01235"
     CDS             complement(5448..5639)
                     /locus_tag="VD737_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09213.1"
                     /translation="MNLTWIGRNRSLELSPRARLVDDVAAEALRLKREQLQWRPRNGF
                     LVDEAPTDGDANQATDRAA"
     gene            complement(5931..6614)
                     /locus_tag="VD737_01240"
     CDS             complement(5931..6614)
                     /locus_tag="VD737_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HXW09214.1"
                     /translation="MNVSDAVASRISCRAFLDTPVPGAVVREILDAARRAPSGGNLQP
                     WWVYALAGAPLAELRARVRREVQDDPRGDGSHEYDIYPPGLGEPYRSRRYKAGEDLYA
                     TLGIERQDKASRLRQLARNYEFYDAPVGLFFYVDRSLGPPQWSDVGMYMQTVMLLARE
                     RGLHTCAQEIWSLVPRTVATCLDAPAHLMLFSGMAMGHADPAHPINSLRTERAPLEEF
                     ARLQGFDRV"
     gene            6733..7239
                     /locus_tag="VD737_01245"
     CDS             6733..7239
                     /locus_tag="VD737_01245"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HXW09215.1"
                     /translation="MHELRQYVADVPDFPKPGILFRDITPLLRAHFGATVEALDALWG
                     EDEWRSIDVVAGIESRGFILAAALAERRDKGFVPIRKKGKLPPPVVDVAYDLEYGTGV
                     LEMQAGQGRLLLVDDVLATGGTLGASVTLSRAAGYSVHGIGVLIDIGIAPGFRAEEHV
                     PRTVLHYP"
     gene            7265..8497
                     /locus_tag="VD737_01250"
     CDS             7265..8497
                     /locus_tag="VD737_01250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HXW09216.1"
                     /translation="MIPVASSPRRRAALVFIFVTVVLDMLALGMILPVLPHLIEDFLG
                     GDTARAAKIFGLFGSVWAVMQFVSMPVMGGLSDRFGRRPVILLSNLGLGLDYVLMALA
                     PNLRWLFVGRVISGVTAASISTAMAYIADVTPPENRAKSYGLVGMAFGLGFIVGPALG
                     GVLGSIDPRLPFWAAAAASLANAAYGYFVLPESLPPERRRAFEWKRANPAGSLKFLRA
                     HREITGLAAAAFLSAVAHAVLPAVFVLYAGYRYGWDEKTVGLALAVVGASSVVVQGTL
                     VGPLVRRFGERRVLLAGLLAGAIGFIVYALAPTGAWFLAGIPVVALWGLAAPSAQGLM
                     TRHVGANVQGELQGAMGSLQGIATVIGPVMFATIFAWSIEAGRQWHFPGAAYTLAALL
                     LVASATIVARAARDRSVA"
     gene            8494..11388
                     /gene="glnE"
                     /locus_tag="VD737_01255"
     CDS             8494..11388
                     /gene="glnE"
                     /locus_tag="VD737_01255"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HXW09217.1"
                     /translation="MTPPVEVPAMLASRVDELREQLAGRAPLPRTPAGDTLESTLRYA
                     WACSAFVADACLRDEGLLPWLAADGRLLEGVEAAWFEAGVEDATAPGTTDDTVFMVAL
                     RRFRRRHLVRIAWRDLAGLAQIETVLAELSALADACIAAACRHATATLGRRHGVPRDD
                     QGRDMPLLVLGMGKLGGGELNFSSDVDLVFTFAEHGETAGPRPLSHEEFFTRVGKRVS
                     QLLGTPTDEGFVYRVDLRLRPFGDSGPVAVSYDAIEDYLQQHGRDWERYAYVKARPVF
                     GAAAGFDELYRDVLRPFVYRRYLDFGVFESLRGMKELIAREVERRELQQNVKLGPGGI
                     REIEFIVQAFQLLRGGSNPRLQTRRLLEALPLLAGQKLLPTAAVGELQAAYRFLRRVE
                     NRLQEWNDEQTHELPEDATARARLALAMGAPDWPALLRELDVHRQLVSAHFRRAVFAP
                     DQGDPGNDGDGSALEHLLDAELDDTRRRELVATLAVPATVADALLGEFERLRDGAYYR
                     RLDETGRRRLHTLLPGLLRSLVRLPNPEATLGRVLGIVERIGGRTVYLALLNENPKAR
                     ERLIELCSHSRFLADQIAAFPLLLDELLDERLFDTTPTRAELELELRARMSATNPGDQ
                     EQQVDALREFQRAAMFRIAVPDLTGRLPLMKVSDRLTELAELLVQESLDLAWQQITAR
                     HGVPMCGPGESQLVPARMVVIAYGKFGGIELGYGSDLDLVFLHDSHGEVQRTTGPLVV
                     ENGVFFLRLVQRLVHLLTVHSAAGRLYEVDTRLRPSGKGGLLVQSIEGFADYQRAEAW
                     TWEHQALLRSRAVAGPDELQARYEALRVELLRSAVRRSSLREDVRAMRDRMRAELSRS
                     RPGEFDLKQDAGGITDVEFLAQYWSLRWAERHAELVTYSDNIRQLESLASICLVPQQT
                     VDLLTAAYRTYRQRLHHLSLDGLGNVAPADEFASTRAAVIEVWRTTMESG"
BASE COUNT         1629 a         4013 c         3991 g         1768 t
ORIGIN      
        1 ggccggcacc gtacgggcgc gcaccttcga attcctctca tggcacgcat gagcaaaaaa
       61 cgcaccatgc ctgggcgcga cggctcgtcg tgtgcctaat atccagcgat gcgtgctcct
      121 cgcgacgaac gtcgcttgcg ccccgcgcct gcgcgaagca ggacggcctg gtggctggtg
      181 gtcctcgcgg cgatcgtcgt cgcgctggcg gtggccgcgt atctgtacag ccggacccct
      241 cccgaaaccc tgccccgcca gccggcgacg ccgatccagc cccaggccga cgagtcgccg
      301 cggcacccga ttgcgcctgc acccgccgac gccgcacggc cgcctctgcc ctcgctccgc
      361 gaaagcgatc cggtggtgat gagtgcggca acggatctcg tccctccgtc ggcgcgcgac
      421 ctgctgcaga ccgaagacat ggtgcgacgc tgggtgatca cggtcgacgc gctgccgcgc
      481 gcgcgcctgc cgcagcgagc gagaccagtg cagcgagtcc ccgaccagct ggtggcgggc
      541 ggtgcgccgg acgcgccgac gctgtcgccg gacaactatg cgcggtacga accgtatgtg
      601 gcactcgcgg agtcgatcga tccgcaggca ctggtgagcg cctaccgctg gctgtacccg
      661 ttgttccagc aggcgtacac cgaggtggtc cgcccggaag gctacttcaa cgaccggctg
      721 atcgaggtga tcgaccatct gctggcgacg cccgaactgc ccgcagagcc ccagctgagc
      781 cgaccgagcg tgacgtaccg actggccgat ccggatctcg aggcgctctc ggccggtcgc
      841 aaggcgctgc tgcgcatggg cccggacaac gcgcggcgcg tcaaggcggt gctggcgcgg
      901 ctgcgtgccg agctgtcggc cgactcgccc gcacgcgccg gccagggagt ccaggacgac
      961 ggctgtagcc ggggccgtac gaaaaaggcg gcgggcccgt gcccgccgcc ttcgttcgtg
     1021 gatgcgtcgg cggaggttac tggatcagtt cgttgcgcag cgcgtacgcg gtcagatccg
     1081 cgttgctctt catggccatc ttgtcgagca cgcggcgccg gtaggtgctc acggtcttga
     1141 cgctcaggaa catcttggcg gcgacctgcg acacggattc gccctgcgcg agcttgcaga
     1201 agatctggaa ctcgcgggcc gacagccggc tgtgcaacgg cccctgcgga tcctcgccgc
     1261 tcgccgaggc gaccagctgc tccgccagcg ccgggcccag gtaccgcccg ccgcggatga
     1321 ccatgcgcat cgcgcgcagc agttccgccg gctcgacgcc cttggcgatg aatcccgccg
     1381 cgcccgcacg cagcacgttc aggccgtatt gcgcctcgga ataggcgctc agcatcagca
     1441 cccgcgtggt ggactggtgc gtctgcagcg accgcaacac gtcaaggccc gacatgtcgc
     1501 cgcccagctg caggtcgagc agtacgagct tcggctggtg ctcttgaatg gccgtcaggc
     1561 ccttgcgacc ggtgagcgcc tcggcgacga cccgcatgtc cggctgggag tcgatgatgc
     1621 tgcgaaggcc tgcgcagatg acgtggtggt cgtcgatgat gacgacggga atcttgttga
     1681 gcatggtcgg tctcccttct gtaggacgga gcttgccgcg gcccgccgct ggcggaaagc
     1741 aggcttcgct tcccgaatgt cgtaggaacg cccctacgcc gctgacaccg atcggtcgtc
     1801 agcgcctcgg ttgatcgccc accctgctgc atccactgcg acggtctgcc gcgtcaccgt
     1861 ggcaagatgc ccgccatgca gctcggctgg ctttcgctcg caatcacggc cacggtcgct
     1921 tatcacctcg tcctcaagct gacgccggcg ggcgccaatc cgtttctgac gctgatgctg
     1981 acctatgccg tcgtgacgct ggcgttcggc gtggggctcg ccctcggtcc cgggccgttc
     2041 gagtggcgac aggaactgcg cgcgctcaac tggacggcgg tggcgctagc cgtcgcgatc
     2101 gtcgcgctcg acctcggctt cctgttcctc taccgcagcg gctatgacgt cagcctcggc
     2161 gcgctcgtga cgcaatcgac cgccgcgctg ctgctgctcg tgatcggcgt gctggtgttc
     2221 cgagagcggc tgagcgtcgc caacggggtc gggctggcgc tgtgcctggc ggggctttgg
     2281 ctggtgaaca ggagatagtc accgaccact agccactctc ctcgtccttc tcttccgccc
     2341 ggatcgtccg cacggtccac cacaccagcg ccgtgacgac ggcgagcatc gccgccatca
     2401 gcagcgccat cttcgggccc gccaggcgcc aggcctcgcg cagtccgaag atcacgccga
     2461 tcggcgtggt gaccagcagg atcggtcggg cgatacgcac gcgcttcagg gctcgcgcat
     2521 cttccagccc gacggcggca tggcgtcgaa gaacatctgc acccggtgca ggagattatt
     2581 ccggtcggcc cagaccacct gttcgtcgag ccggtccatg cgatcctgct cggaggggtc
     2641 ctggtcgagg ccgtgccaca tcggcggcgg gggaggcgtc ttgccgcgct tctccgcctc
     2701 ttccgacaac tgctggtaca ggcgttccca gtaacgcatg gtcggatacg cacggccgcg
     2761 caaccgatga tggtcgagca ggcgccgggc gcgcgacgac gtgcggagtc cggtgggcat
     2821 ggggtgtccc tcgacgacag cgtagccgct ccgccgtcgg cgcgaaaccg gctcgtaagc
     2881 gccgtgtggg gtatgctcgg gcgaaacgag gtcatcgacg ggaggcgtgc gcatgacgat
     2941 tgcgtactgg tgcgtgctga tcgcggggct gctgccctac gtatcgacgc tggcggcgaa
     3001 gatcgggggg gagcgtttcg acaaccgcaa tccgcgcgac tggctcgcat cccaatccgg
     3061 tttccggggc cgcgccaatt cggcgcaact gaacggcttc gaagccttcc cgctgttcgc
     3121 ggccggcgtc gtgatcgcgc agctcgccgg ggccgcaccg tcgcgcgtcg acgagctcgc
     3181 catggcattc gtcgcggcgc gcgtgctgta cacgctgttc tacttcgcgg acatggcgct
     3241 gctgcgctcc ctggcatggt cggtcggtat cggctgcagc gcggcgctgt tcttcatcgc
     3301 cgcctgaccc gagccggatg gcccgcatgg atgccgaacc cgacagcctc gaacgcctgc
     3361 gcagctttcg agaggaatac cgccggcggc acgtgccacg cggctatcgc ggcggccgcc
     3421 acctgttctt cacgttcggc ctcggcagcg ccgcgctctt ggcgtgcctg ctgcaactcg
     3481 agcaggtgca gccgctcgag tgggcgacgg tgccgctgac ggcgctgtac tcgaacctcg
     3541 cggaatactt cggccaccgc gggccgatgc atcaccgtcg gccgggcctc gggctggtct
     3601 tcgagcgcca cgcgcggcag caccatcgct tcttcacggc cgccgcgatg ccggtggaca
     3661 cgctgcagga tctccgggcg gtgctgttcc cgccggtgct gatgacgttc ttcctggccg
     3721 cgttcgcgct gccggtcgcg tggctgctgg cggtggccgt ctcggcgaac gtcgcgtggc
     3781 tgttcggcgc gacgtcgctc gcctacttcc tcaactacga gttcctgcac ctcgcctacc
     3841 acctgccgcc gcgatatgcc gtggcgcgct ggccgctggt cgggcgcctg aagcgcctgc
     3901 acgagacaca ccacgacccg cgactgatga cgcactacaa cttcaacatc agctatccgc
     3961 tcggcgactg gctgttccgc accctgcgct gagcctcagg cccgccaacg gcggttgcgc
     4021 cagagcccgt ccaccgcgta gatcgcgagc gccgcccaga tgaacagaaa gccgatgccg
     4081 cgttcgcgcg aaaacggctc gtggaagatc caaacgccgg tcaggaactg cagcgtcggg
     4141 ccgatgtact gcaccagccc gacggtcgac agcggtatcc gccgcgcgcc gtaggcgaac
     4201 agcgcgagcg gcagcgccgt gatcaggccg ctgccgacga gcatcgcgtt cacgtgcggt
     4261 gcggcgcggc cgagtgcgcc aagcccgcgc cattcgagcc acagcagcca ggcgagcgcg
     4321 aacggcgtga gcagcagggt ctcggttgcg agccccggca ccgcgtcgac cgcgacgacc
     4381 ttccggatca gcccgtacag gccgaagctc gccgccagca cgagggcgat ccagggcggg
     4441 cgaccggcaa ctacggccag gtagaccacg ccgaccaccg cgaggccgac ggcgaactgc
     4501 tgcggccggt tgagcctctc gcccagcacc atgacgccga gcaccacgct gaacagcggg
     4561 ttgatgaagt agccgaggct cgagtcgatc acgtggtcgt ggttgacggc ccagacgtag
     4621 acgagccagt tgaggctgat cagcgtcgcg ctcgcgacca gccgcagccg tacgccacga
     4681 ttggccagcg cggcgcgcac cgcgtgcagc tcgccgcgca gcgcgagcca cgcgaacacc
     4741 agcaggcagc accatacgat ccggtgcgcc atgatctcga gggccgattg cgcctgcagc
     4801 ggccgcaggt acagcggcat cgcaccccag atcgtgaacg ccgcgatcgc cgcggcgagc
     4861 ccggcgccgg aactcgcgga cgacgggtcg gggccgttcg gtggaacgtc tgcgcggcgc
     4921 atggggtcgc gcattatgca gaaagcgcgg cggcgcgtgg cgaccgcgcc gtgtgccggg
     4981 ccactaggcc ggtcgtggaa actccggagc gccgccgcgt ggccggcact gcaagcttcc
     5041 ggcatggaca accgactcga ccagggccgt ctcgctcgtg agtggcgcac catgcaggcg
     5101 atggtggccg tctactgcca cgggcacggg cacgtcgcgg cggccggctg caagctctgc
     5161 gcggactgcg cgcgatttct cgactacgcc ggaaggcgcc tggaaaagtg tccctacggc
     5221 gagtccaagc ccacctgcgc gaagtgtccg atccattgct acaagcccga gccgcgcgcg
     5281 ctggcgcgac aggtcatgcg gtactccggc ccgcgcatgg cgtggcgaca cccctggctg
     5341 agcctgactc acctcaccga caagctgcga cgggtggagc acccgatgac ggcgcgtcgc
     5401 cgcgaacgcc gggtgacggc agcgcgcggc gagtgaatcg cgcggcctca ggccgctcga
     5461 tcggtcgcct ggttcgcgtc gccgtcggtg ggcgcttcgt cgaccaggaa tccgttgcgc
     5521 ggacgccatt gcagctgctc gcgcttgagg cgaagcgctt cggccgcgac gtcgtcgacg
     5581 agccgggccc gtggcgagag ttcgagactg cggttgcgac cgatccaggt caggttcatt
     5641 gacttaatct ccctcgcgaa ttgcctgcta caaggcccgt tgcacgggcc gtgccagtcg
     5701 cacccccacc gccgcatgcg gtgctccgtg cgtggatcgg ggcgagcgtc gcgatccccg
     5761 ggcgcacctt cgtaaagtcc cgcgacacgt cagttgttgc gccgaagcgt caaccagccc
     5821 ccgaaccgtg aggtacgtcc cacttattga gcgcttcggc gggtggcggc gacataagac
     5881 ggcacgcttc gctcgcgtcg cgtgtcagtt aggccgacat aggcggacgc ctacactcgg
     5941 tcgaagcctt gcagtcgcgc gaattcctcg agcggcgcac gctcggtgcg cagcgagttg
     6001 atcgggtggg ccgggtcggc gtggcccatc gccatgccgg agaacaacat caggtgcgcc
     6061 ggcgcgtcga ggcaggtggc gaccgtccgc ggcaccagcg accagatctc ctgggcgcag
     6121 gtgtgcaggc cgcgttcgcg cgcgagcaac atgacggtct gcatgtacat gccgacgtcc
     6181 gaccactgcg gcggtcccag cgagcggtcg acatagaaga agagcccgac cggcgcatcg
     6241 tagaactcgt agttccgcgc cagctggcgc aggcgtgacg ccttgtcctg gcgctcgatg
     6301 cccagcgtcg catagaggtc ttcgcctgcc ttgtagcgac ggctgcggta aggctcgccg
     6361 aggccgggtg ggtagatgtc gtattcgtgc gagccgtcgc cgcgcgggtc gtcctgcacc
     6421 tcgcgccgca ctctcgcgcg cagctcggcg agcggcgcgc cggcgagcgc gtacacccac
     6481 cacggctgca ggttgccgcc cgacggcgcg cgccgggccg cgtcgaggat ctcgcgcacg
     6541 accgcgcccg gcaccggcgt gtcgaggaac gcgcggcaac tgatgcggct cgcgacggcg
     6601 tcactgacgt tcacgggtat ccctcctcgc ggccggcgat tctgccagaa gcggactcgc
     6661 cgccacgtgg cctgcacgac tcgcggcccc cccgggattt ggttaccctg cgcgcgccga
     6721 gccgaggtgt gcatgcacga gctgagacag tacgtcgccg acgtacccga ttttcccaag
     6781 cccgggatcc tgttccgcga catcacgccg ctgctgcgcg cgcacttcgg ggcgaccgtc
     6841 gaggcactcg acgcgctgtg gggcgaggac gagtggcgca gcatcgacgt ggtggccggc
     6901 atcgagtcgc gcggcttcat cctcgcggcg gcgctggccg agcgccgcga caagggcttc
     6961 gtgccgatcc gcaagaaggg caagctgccg ccgcccgtgg tcgacgtggc gtacgacctc
     7021 gaatacggca ccggggtgct cgagatgcag gccgggcagg gccggctgct gctggtcgac
     7081 gacgtgctcg cgacgggcgg cacgctcggc gcgtcggtca cgctgagccg ggccgcgggc
     7141 tactccgtgc acgggatcgg cgtgctgatc gacatcggca tcgcgccggg tttccgcgcc
     7201 gaggagcacg tgccccgcac ggtgctgcac tacccgtgac cagcggcgtg cgcgcggcac
     7261 gccgatgatc ccggtcgcca gttcgccgcg tcgtcgcgcg gcgctcgtct tcatcttcgt
     7321 caccgtcgtg ctcgacatgc tcgcgctcgg catgatcctg ccggtgctgc cgcacctgat
     7381 cgaggacttc ctgggcggtg acaccgcgcg ggcggcgaag atcttcggcc tcttcgggtc
     7441 ggtctgggcc gtgatgcagt tcgtgtccat gccggtcatg ggcggccttt ccgaccggtt
     7501 cgggcgtcga ccggtgatcc tgctctcgaa cctcgggctc gggctcgact acgtgctcat
     7561 ggcgctggcc cccaacctgc gctggctgtt cgtcgggcgc gtgatctcgg gcgtgacggc
     7621 cgcgagcatc agcacggcga tggcgtacat cgcggacgtg acgccgcccg agaaccgggc
     7681 gaagagctac ggcctggtgg gcatggcctt cggcctcggg ttcatcgtcg gccccgcgct
     7741 cggcggcgtg ctcggctcca tcgacccccg attgcccttc tgggccgccg ccgcggccag
     7801 cctcgccaac gcggcctacg gctacttcgt cctgcccgag tcgctgccgc ccgagcgtcg
     7861 acgcgcgttc gaatggaagc gggccaatcc cgccggctcg ctcaagttcc tgcgcgcgca
     7921 tcgcgagatc acgggcctgg ccgccgcggc gttcctgagc gccgtggcgc atgccgtgct
     7981 gccggccgtg ttcgtgctgt atgccggcta ccggtacggg tgggacgaga agacggtggg
     8041 cctcgcgctc gcggtcgtcg gcgcgtcctc ggtcgtcgtg cagggcacgc tcgtcggccc
     8101 gctcgtgcgc cggttcggcg agcggcgcgt gctgctcgct ggactgctgg ccggcgcgat
     8161 cggcttcatc gtgtacgcgc tcgccccgac gggcgcgtgg ttcctggcgg gcatcccggt
     8221 ggtcgctcta tggggactcg cggcgccgag cgcccagggg ctgatgacgc gccacgtcgg
     8281 cgccaacgtg cagggcgaac tgcagggcgc gatgggcagc ctgcagggga tcgcgaccgt
     8341 gatcgggccg gtcatgttcg cgaccatctt cgcgtggtcg atcgaagccg gtcggcagtg
     8401 gcactttccg ggcgcggcct acacgctggc cgcgctgctg ctggtggcct ccgccacgat
     8461 cgtcgcgcgc gcggcacgcg acaggagcgt ggcttgacgc cgccggtcga ggtgccggcg
     8521 atgctcgcga gcagggtgga cgagctccgc gagcagctgg ccggccgcgc gccgttgccg
     8581 cgcactccgg ccggtgacac gctcgagtcg acgctccgct acgcgtgggc ctgcagcgca
     8641 ttcgtcgccg acgcctgcct ccgcgacgag gggctgctcc cctggctcgc cgcggacggg
     8701 cggctgctcg aaggcgtcga ggcggcctgg ttcgaagccg gtgtcgagga cgcgaccgcg
     8761 cccggcacga cggacgacac ggtgttcatg gtggcgctgc gacgcttccg acgcaggcac
     8821 ctcgtgcgca tcgcgtggcg cgacctcgcg ggcctggcgc agatcgagac cgtgctcgcc
     8881 gagctctccg cgctcgcgga cgcgtgcatc gcggcggcct gccggcacgc gacggcgacg
     8941 ctcggccggc gccacggcgt gccgcgcgac gaccagggcc gggacatgcc gctgctggtg
     9001 ctgggcatgg gcaagctcgg cggcggcgag ctcaacttct cgtccgacgt cgacctcgtg
     9061 ttcaccttcg ccgaacacgg cgagaccgcc ggtccacggc cgctctcgca cgaggagttc
     9121 ttcacgcgcg tcggcaagcg cgtgtcgcaa ctgctcggca cgccgaccga cgagggcttc
     9181 gtctaccggg tcgacctgcg cttacgaccg ttcggcgact cgggcccggt ggccgtcagc
     9241 tatgacgcga tcgaggacta cctgcagcag cacggccgcg actgggagcg ctacgcctat
     9301 gtcaaggcgc ggcccgtgtt cggcgcggcc gccgggttcg acgagctcta ccgcgacgtg
     9361 ctgcggccgt tcgtctaccg acgctacctc gacttcggcg tcttcgagtc gctgcgcggc
     9421 atgaaggagc tgatcgcgcg cgaagtcgaa cggcgcgagc tgcagcagaa cgtcaagctc
     9481 ggtccgggtg gcatccgtga aatcgaattc atcgtgcagg cgttccagct attgcgcggc
     9541 ggcagcaacc cgcgactgca gacccggcgc ctgctcgagg cgttgccgct gctggcgggg
     9601 cagaagctgc tgccgacggc cgcggtcggc gaactgcagg ccgcctaccg gttcctgcgg
     9661 cgcgtcgaga accggctgca ggagtggaac gacgagcaga cgcacgagct gccggaggac
     9721 gcgacggcac gcgcgcgcct ggcactcgcg atgggcgcgc cggactggcc cgcgctgctg
     9781 cgcgagctcg acgtccaccg ccagctggtg agcgcgcact ttcgtcgcgc ggtgtttgcg
     9841 cccgaccagg gcgatcccgg caacgacggc gacggcagcg cgctcgagca cctgctcgac
     9901 gcggaactcg acgacacacg ccggcgcgaa ctcgtcgcga cgctcgccgt cccggcgacg
     9961 gtcgccgacg cgctgctggg agagttcgag cgcctgcgcg acggagccta ctaccggcgg
    10021 ctcgacgaaa ccggccgtcg ccgcctgcac acgctgctgc cgggcctgct gcgttcgctc
    10081 gtgcgcctgc cgaaccccga agcgacgctc ggccgcgtgc tgggaatcgt cgagcggatc
    10141 ggcgggcgca cggtctacct cgccctgctc aacgagaatc cgaaggcgcg cgagcgcctg
    10201 atcgaactct gctcgcactc caggttcctg gccgaccaga tcgcggcctt cccgctgctg
    10261 ctggacgagc tgctcgacga gcgcctgttc gacacgacac cgacgcgcgc ggaactggag
    10321 ctcgagctgc gcgcccgcat gtccgcgacc aatcccggcg accaggaaca gcaggtcgac
    10381 gcgctgcgcg agttccagcg tgccgcgatg ttccgcatcg cggtgcccga cctgacgggt
    10441 cggctgccgc tcatgaaggt gagcgaccgg ttgacggagc tcgccgagct gctggtgcag
    10501 gaatcgctcg acctcgcctg gcagcagatc accgcgcgac acggcgtgcc gatgtgcggc
    10561 cccggcgaat cgcagctcgt gccggcgaga atggtcgtca tcgcgtacgg caagttcggc
    10621 ggcatcgagc tcggctacgg ctccgacctc gacctggtgt tcctgcacga ctcgcacggc
    10681 gaagtgcagc gcacgacggg gccgctggtc gtcgagaacg gggtgttctt cctgcggctc
    10741 gtgcagcggc tcgtgcacct gttgacggtg cattccgcgg ctggccgcct gtacgaggtc
    10801 gacacgcggc tgcggccgag cggcaagggc ggcctgctgg tccagagcat cgagggcttc
    10861 gccgattacc agcgcgccga ggcgtggacc tgggagcacc aggcgttgct gcggtcgcgc
    10921 gccgtcgccg ggccggacga gctgcaggca cgctacgagg cgttgcgcgt cgaactgctg
    10981 cgcagcgcgg tgcgccgcag ctcgctgcgc gaggacgtac gcgcgatgcg cgaccgcatg
    11041 cgcgccgaac tgtcgaggtc acgaccgggc gagttcgacc tcaagcagga cgcgggcggc
    11101 atcaccgacg tcgagttcct cgcgcagtac tggtcgctca ggtgggccga gcgacacgcc
    11161 gagctcgtga cctactccga caacatccgc cagctcgaga gcctcgcctc catctgcctc
    11221 gtgccacagc agaccgtcga cctgctgacg gccgcgtacc gcacgtaccg ccagcgactg
    11281 caccacctgt cgctcgacgg cctcggcaac gtcgcgccgg cggacgagtt cgcctcgaca
    11341 cgcgcggccg tgatcgaggt gtggcggacg acgatggaaa gtggctagtg gctagtggtc
    11401 a
//
LOCUS       DASPFR010000052         3919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1726688, whole genome shotgun sequence.
ACCESSION   DASPFR010000052 DASPFR010000000
VERSION     DASPFR010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1726688"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VD737_01260"
     CDS             complement(<1..919)
                     /locus_tag="VD737_01260"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HXW09218.1"
                     /translation="MRSMLFVPGDRPERFGKAVRSGADAVILDLEDAVTADSRPQARR
                     NVADFLRDGARDVPVWVRINPIDSSDALVDVAAVVPARPDGLVLPKTRTVADLWRLDH
                     WLEALEAAHGLPAGSIGVVALVTETAHALLHAAGFATPPKRVTGYTWGAEDLAAEVGA
                     TANRTADGEYEGVYRIARASCLLMAAAANVAAIDTTDVEFRDVAAVERRARASRRDGF
                     VGKLVIHPAQVAPIHAAFSPSAEEVTWARRVVEALAQPAGAGAVAIDGRMVDRPHLRQ
                     AERILAAARASDVATGSSPRFSTEKGGQPP"
     gene            complement(919..1386)
                     /locus_tag="VD737_01265"
     CDS             complement(919..1386)
                     /locus_tag="VD737_01265"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXW09219.1"
                     /translation="MAMGGLYYEQFSVGRRFEHVLRRTVTEADNLIFSTLTHNPAALH
                     LDEEAMKTSEFGTRIVNSCFTLSLMVGISVADTTLGTTIANLGWDEVRFPRPVFIGDT
                     LRIETSVLEMRESRSRPDSGVVTFEHRAFNQRNEEVGRCKRIALMKKRPQETA"
     gene            complement(1415..2578)
                     /locus_tag="VD737_01270"
     CDS             complement(1415..2578)
                     /locus_tag="VD737_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005262059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HXW09220.1"
                     /translation="MTMLEFDFGLGEDIDLLRETVRNFAASEIAPRAAAIDRDNAFPR
                     DLWPKLGELGLLGITVEHEYGGSGLGYLAHVVAMEEISRASASVGLSYGAHSNLCVNQ
                     LRRWGTPEQKSRYLPRLVSGEHLGALAMSEPGSGSDVMSMRLHAEPRGDRYVLNGNKM
                     WITNGPTADVLVVYATIDPGAGNKGVTAFLVERGFAGFSTAQKLDKLGMRGSDTCELV
                     FENCEVPAENVLGPVGGGARVLMSGLDYERVVLAGGPLGIMRACLDVVLPYVHDRKQF
                     GAPIGTFQLMQAKLADMYTTLSSCAAYTYAVARACDAGKASRIDAAGCILLAAERATW
                     MALEAIQSLGGNGYINDYATGRLLRDAKLYEIGAGTSEIRRMLIGRELFLATP"
     gene            2654..3424
                     /locus_tag="VD737_01275"
     CDS             2654..3424
                     /locus_tag="VD737_01275"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXW09221.1"
                     /translation="MSESPVPAYKAPLFEPLTRQSASLRISTAIEQKILRRNLRPGDA
                     LPTEGELAEQFAVHRSTVREALRRLESAGLVGRQGGSKKLRVTRPGHAETASRVGRTL
                     ALDDVTFIELWEAMLAVAPRTAALAATRGNAQSVATLEAIVNAVETARGADAAVGWVA
                     DFFGKLAEMSGNRVLMLAMQPVMTLLAPSLRRMIDRVPQGRTRILVAQRCVVEAIRTG
                     KPDEAEAWMTRHVQDFRRGYELAGIALDTRVGTTPVTD"
     gene            complement(3482..>3919)
                     /locus_tag="VD737_01280"
     CDS             complement(3482..>3919)
                     /locus_tag="VD737_01280"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HXW09222.1"
                     /translation="AEQPDATYPTVLITGRQLEHWHTGSMTRRTSALDAIEPEPVASV
                     HPLDLADLDAAPGDIITIASRRGRITLYARADEGTPRGVVFVPFCYYEAAANRLTNPV
                     LDPFGKIPEFKYCAIRVTPGGEVPPRLSFGGGVLRAGDPAVRS"
BASE COUNT          565 a         1374 c         1368 g          612 t
ORIGIN      
        1 cggggggctg tccccctttt tcggtagaga aacggggact gctccccgtc gcgacgtcac
       61 tggcgcgggc cgccgcgagg atccgctccg cctgtcgcag gtggggccgg tcgaccatcc
      121 tgccgtcgat cgcgacggcg ccggcgccgg cgggttgcgc gagcgcctcg acgacgcgac
      181 gcgcccacgt cacctcctcc gcggaagggc tgaacgcggc gtggatcggc gcgacctgcg
      241 cgggatggat cacgagcttg ccgacgaatc cgtcgcggcg cgaagcgcgc gcgcgtcgct
      301 cgacggccgc gacgtcgcga aactcgacgt cggtcgtgtc gatggcggcg acgttcgcgg
      361 cggcggccat cagcaggcac gacgcgcggg cgatccggta tacgccctcg tactcgccgt
      421 ccgcggtgcg gttcgcggtc gctccgactt cggccgccag gtcttccgcg ccccaggtgt
      481 atccggtgac gcgtttcggt ggcgtggcaa aacccgcggc gtgcagcagg gcgtgggccg
      541 tctcggtcac gagcgcgacc acgccgatcg agccggcagg cagaccatgc gcggcctcca
      601 gggcttcgag ccagtgatcc aggcgccaga ggtcggccac ggttcgggtc ttgggcagga
      661 ccaggccgtc gggacgcgcc ggcaccaccg cagcgacgtc gacgagcgcg tcgctcgagt
      721 cgatcgggtt gatgcgtacc cagaccggga cgtccctggc gccgtcgcgc aggaagtcgg
      781 ccacgttgcg acgcgcctgc gggcgactgt cagccgtgac ggcgtcctcg aggtcgagga
      841 tgaccgcatc cgcgccgctg cgcaccgcct tgccgaagcg ctcgggccgg tcgcccggca
      901 cgaacagcat cgagcgcatc acgcggtctc ctgcggacgc tttttcatga gcgcgatccg
      961 cttgcagcgg ccgacttcct cgttgcgctg gttgaatgcg cggtgttcga acgtgacgac
     1021 cccgctgtcc ggccgcgacc ggctttcacg catctcgagc acgctcgtct cgatccgcag
     1081 cgtgtcgccg atgaacaccg ggcgcgggaa ccgcacttcg tcccagccca ggttcgcgat
     1141 cgtcgtgcca agcgtggtgt cggccacgga gatgccgacc atcaggctca acgtgaaaca
     1201 gctgttgacg atgcgcgtgc cgaactcgga cgtcttcatc gcctcctcgt cgaggtgcag
     1261 cgccgccggg ttgtgcgtga gcgtgctgaa gatcaggttg tcggcctcgg tcaccgtcct
     1321 gcgcagcacg tgctcgaagc gccggccgac gctgaattgc tcgtagtaga gcccgcccat
     1381 cgccatgtcg gtggtcctcc gctcgcgcgt gcggtcaggg ggttgccagg aacagttcgc
     1441 gcccgatcag catgcggcgg atctcgctgg tgccggcgcc gatctcgtac agtttcgcgt
     1501 cccgcagcag gcggccggtc gcatagtcgt tgatgtagcc gttgccgccc agcgactgta
     1561 tcgcctcgag cgccatccag gtggcgcgtt cggccgccag caggatgcag ccggcggcgt
     1621 cgatgcgcga ggcctttccg gcgtcacagg cacgggcgac cgcataggtg tacgccgcgc
     1681 acgaactgag cgtcgtgtac atgtccgcga gcttggcctg catcagctgg aacgtgccga
     1741 tcggcgcgcc gaactgcttg cggtcgtgca cgtacggcag caccacgtcg aggcaggcgc
     1801 gcatgatgcc gagcggtccg ccggccagca cgacgcgctc gtagtcgagg ccgctcatca
     1861 gcaccctggc gccgccgccg accggtccga ggacgttctc ggccggcacc tcgcagttct
     1921 cgaacaccag ctcgcatgtg tcggagccgc gcatgccaag cttgtcgagt ttctgcgccg
     1981 tcgaaaaacc cgcaaacccg cgctcgacca ggaacgccgt gacgcccttg ttgcccgcgc
     2041 ccgggtcgat cgtcgcgtag acgacgagca cgtccgccgt ggggccgttg gtgatccaca
     2101 tcttgttgcc gttgagcacg tagcggtcgc cccgcggctc ggcgtgcagg cgcatgctca
     2161 tgacgtccga gcccgaccca ggctcgctca tcgcgagcgc gccgaggtgt tcgccgctca
     2221 ccagcctcgg caggtaacgt gacttctgtt ccggcgtgcc ccagcggcgc agctggttca
     2281 cgcacaggtt cgaatgcgcg ccgtaggaca gtcccacgct cgccgacgct cgactgatct
     2341 cttccatcgc gacgacgtgc gcgaggtaac cgagccccga gccgccgtac tcgtgctcga
     2401 ccgtgatgcc gagcaggccg agctcgccca gcttcggcca gaggtcgcgg gggaacgcgt
     2461 tgtcgcggtc gatggccgcg gcacgaggcg cgatctcgct cgccgcgaaa ttgcggacgg
     2521 tctcgcgcag caggtcgatg tcttcgccga ggccgaagtc gaactcgagc atggtcatcc
     2581 ccggagcttg caggttgatc gatcggtgcc ctatattgtc cgacaattag actatccgtc
     2641 aacggagtcc gccgtgagcg agtcgcccgt ccccgcctac aaggcgccgc tgttcgaacc
     2701 gctgacccgc cagtcggcct ccctgcgcat ctcgactgcc atcgagcaga aaatcctgcg
     2761 ccgcaacctg cggcccggcg atgcgctgcc gaccgagggc gagctcgccg agcagtttgc
     2821 ggtgcatcgt tccaccgtcc gcgaagccct gcggcgcctc gagtccgctg gcctcgtcgg
     2881 ccgccagggc ggcagcaaga agctgcgcgt gacgcggccc ggccatgccg aaacggcgtc
     2941 gcgcgtcggc cgcacgctcg cgctcgacga cgtgacgttc atcgaactgt gggaggcgat
     3001 gctggcggtc gcgccgcgca cggcggcgct ggcggcgacg cgcggcaacg cccagtcggt
     3061 cgcgacgctc gaggcgatcg tcaacgcggt cgaaaccgcg cgcggcgccg atgccgcggt
     3121 cggctgggtt gcggacttct tcggcaagct cgccgagatg agcggcaacc gggtcctgat
     3181 gctggccatg cagccggtca tgacgctgct ggccccgtcg ctgcgtcgca tgatcgaccg
     3241 cgtgccacag ggtcgcacgc gcatcctggt cgcgcagcgc tgcgtcgtcg aggcgatccg
     3301 caccggcaag ccggacgaag ccgaggcctg gatgacgcgg cacgtgcagg atttccgtcg
     3361 cggctacgaa ctcgccggaa tcgcgctcga cacccgcgtc ggtacgaccc cggtcaccga
     3421 ctgagcaccg tcggcgacgc ggggccgacg tcacgccggg cccggcgcga tcgatcgggc
     3481 ctcagctccg gacggctgga tccccggccc gcagcacccc gccgccgaag ctcagcctcg
     3541 gcggcacctc accgcccggg gtcaccctga tcgcgcagta cttgaactcg gggatcttgc
     3601 cgaacggatc gagcacggga ttggtgagcc ggttggccgc ggcttcgtaa tagcagaacg
     3661 gcacgaacac gacgccgcgc ggcgtgcctt cgtccgcgcg tgcgtacagc gtgatgcggc
     3721 cgcggcgcga ggcgatggtg atgatgtcgc cgggcgcggc atccaggtcc gcgaggtcga
     3781 gcgggtgcac ggaggcaacg ggctccggct cgatcgcgtc gagcgccgag gtgcgccggg
     3841 tcatcgaccc ggtgtgccag tgttcgagct gacggccggt gatgagcacc gtcggatagg
     3901 tggcgtccgg ctgctctgc
//
LOCUS       DASPFR010000053         2638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1605652, whole genome shotgun sequence.
ACCESSION   DASPFR010000053 DASPFR010000000
VERSION     DASPFR010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605652"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VD737_01285"
     CDS             <1..537
                     /locus_tag="VD737_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HXW09223.1"
                     /translation="LGTDMLPALALGAEPPDRELMQRPPRPAGERLLAWPVLRRAYLW
                     LGVLQAAAALGAFFFVLNAGGWQYGEALAPRDPLYLQATTACLAAIVLAQVANIFSCR
                     DALRSAFRIPLRANPLLLQAVALEILLLGLIVFTPVGQAAFGTAPPPLAAWPIMVALA
                     VCFGLLEELRKAVLRSRT"
     gene            629..1507
                     /locus_tag="VD737_01290"
     CDS             629..1507
                     /locus_tag="VD737_01290"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF006492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HXW09224.1"
                     /translation="MTQAVLGIIGGSGIYDLPGLEDVQERRVHTPWGEPSDLLRTGRV
                     GATRVVFLPRHDRGHRIPPSEINYRANIDALKRLGVTDLVSFSACGSYKEELSPGTFV
                     LLDDFVDRTFRREPSFFGRGCVAHVSFARPVGPALQDLVARAADAEGIRYKRGGTAVC
                     MEGPQFSTLAESLTYQRLGYDVVHMTSMPEAKLAREAEITYACVAMVTDYDCWHETHG
                     PVDVASLLAVIHENGDKARRLFARLAREFPAEHVPCPVGSDRALDAAILTRPEARDPA
                     LLEKLDAVAGRVLRAG"
     gene            1520..2623
                     /gene="mtnA"
                     /locus_tag="VD737_01295"
     CDS             1520..2623
                     /gene="mtnA"
                     /locus_tag="VD737_01295"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HXW09225.1"
                     /translation="MRVHGKPCRTIFRADAAGTVAVIDQTRLPFEFLLRHLHSATDAA
                     AAIRDMVVRGAPLIGVTAAYGVALAMRASPDDENLDRVVEQLAGTRPTAVNLRWALDR
                     ITCVLRATPVAERASRAYAEAAAIADEDVAACEAIGRHGLRVIQEIAARKGGEVVHVL
                     THCNAGWLACVDWGTALAPVYAAHEAGVPLHVWVDETRPRSQGAFLTAFELGAHGVAH
                     TVIADNTGGHLMQHGRVDLCLVGSDRTTATGDVANKIGTYLKALAARDNGVPFYAALP
                     TSTIDWSLTDGVREIPIEERSPEEVTRVRGRTADGSLADVDVVAPGSPVANPGFDVTP
                     ARLVTGIITERGIAAASREGLLRLYPERAERTQ"
BASE COUNT          370 a          942 c          930 g          396 t
ORIGIN      
        1 ctcggcaccg acatgctgcc ggccctcgcg ctgggcgccg aaccgcctga ccgcgaactg
       61 atgcagcgcc cgccacggcc ggcgggggaa cgactgctcg cctggccggt gctgcgccgt
      121 gcctatctct ggctcggcgt gctgcaggcc gccgcggcac tcggtgcctt cttcttcgtc
      181 ctgaatgctg gcgggtggca gtacggcgag gcgctggcac cgcgcgaccc gctctacctg
      241 caggctacga cggcgtgcct cgcggcgatc gtgctcgctc aggtggccaa catcttctcg
      301 tgccgcgatg cgctgcgctc ggcgttccgt atcccgctgc gcgccaatcc gctgctgctg
      361 caggccgtcg cactcgaaat actgttgctc ggcctgatcg tcttcactcc ggttggtcaa
      421 gccgcgttcg gcactgctcc gcccccgctc gccgcctggc ccatcatggt cgcgctggcc
      481 gtgtgtttcg gcctgctcga ggaactgcgc aaggccgtgc tacgtagtcg tacctagaac
      541 gcggccgtcg gtacatccgg agtcccttgg gcccgcggct cgggtaatct ccgcgctcga
      601 tactggcgac gcatcctgac ggcgatccat gacgcaggca gtgctcggaa tcatcggcgg
      661 ctcggggatc tacgacctgc cggggctgga ggacgtgcag gaacggcgcg tgcacacgcc
      721 gtggggcgag ccgtcggacc tgctgcgcac gggccgcgtc ggcgccacac gggtcgtgtt
      781 cctgccgcga cacgatcgcg ggcacaggat tcccccgagc gagatcaatt accgcgccaa
      841 catcgatgcg ctcaagcgcc tgggcgtgac ggacctcgtc tcgttctccg cctgcgggtc
      901 gtacaaggag gaactgagtc ccggcacgtt cgtgctcctc gacgacttcg tcgaccgcac
      961 cttccggcgc gagccttcgt ttttcggccg aggctgcgtc gcgcacgtgt cgttcgcacg
     1021 tccggtcggc ccggccctgc aggacctcgt cgcgcgcgcc gcggacgccg agggcatccg
     1081 gtacaagcgc ggcggcaccg cggtctgcat ggaaggcccg cagttttcga ctctggccga
     1141 gtcgctcacg taccagcgcc tcggctacga cgtcgtgcac atgacctcga tgccggaggc
     1201 caagctcgcg cgcgaagcgg agatcacgta cgcctgcgtc gcgatggtga ccgactacga
     1261 ctgctggcac gaaacgcacg gcccggtgga cgtcgcgagc ctgctcgccg tcatccacga
     1321 gaacggcgac aaggcgcgcc gattgttcgc caggctcgcg cgcgagttcc cggccgagca
     1381 cgtgccgtgc ccggtggggt cggaccgtgc gctcgacgcg gcgatcctga cgcgacccga
     1441 ggcgcgcgat cccgcgctgc tcgagaagct cgacgccgtc gccggacgcg tgctgcgcgc
     1501 cggctgatcg cgccgcccca tgcgcgtcca cggcaagccc tgccgcacca tcttccgcgc
     1561 cgacgccgcc ggcacggtcg cggtgatcga ccagacgcgg ctgccgttcg agttcctgct
     1621 gcgtcacctg cacagcgcga ccgatgcggc ggccgcgatc cgcgacatgg tcgtgcgggg
     1681 tgcgcccctg atcggcgtga cggcggcgta cggtgtggcc ctggcgatgc gggcatcgcc
     1741 cgacgacgag aacctggacc gtgtggtcga gcaactcgcg ggcactcggc cgaccgccgt
     1801 gaacctgcgc tgggcgctcg accggatcac gtgcgtgctg cgcgcaacac cggtggccga
     1861 acgtgcgtcg cgcgcgtacg cggaagccgc cgcgatcgcc gacgaggacg tcgcggcctg
     1921 cgaggcgatc gggcgtcatg gcctgcgcgt gatccaggag atcgcggccc gcaagggggg
     1981 cgaggtcgta cacgtgctca cgcactgcaa cgcgggatgg ctcgcgtgtg tcgactgggg
     2041 cacggcgctg gccccggtct acgccgcgca cgaggcgggc gtgccgctgc acgtctgggt
     2101 cgacgagacg cgtccgcgca gccagggcgc cttcctgacc gcgttcgagc tcggcgcgca
     2161 cggcgtggcg cacacggtga tcgccgacaa caccggcgga cacctgatgc agcacggacg
     2221 cgtcgacctc tgcctggtcg gcagcgaccg caccacggcg accggcgacg tcgccaacaa
     2281 gatcggcacg tacttgaagg cgctggcggc acgcgacaac ggcgtgccgt tctacgcggc
     2341 gttgccgacg tcgacgatcg actggagcct gaccgacggc gtgcgcgaga tcccgatcga
     2401 ggagcgttcg ccggaggagg tcacgcgggt gcgcggccgg acggccgacg gatcactggc
     2461 ggacgtggac gtcgtcgcgc cgggcagccc ggtcgccaac ccgggattcg acgtgactcc
     2521 cgcgcgactc gtcacgggca tcatcacgga acgtggcatc gccgccgcga gccgcgaagg
     2581 cctgctgcgg ctgtatccgg aacgcgcgga gagaacgcag tgaacgaacc ggcgcgtg
//
LOCUS       DASPFR010000054         5407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_140783, whole genome shotgun sequence.
ACCESSION   DASPFR010000054 DASPFR010000000
VERSION     DASPFR010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140783"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..922)
                     /gene="panC"
                     /locus_tag="VD737_01300"
     CDS             complement(62..922)
                     /gene="panC"
                     /locus_tag="VD737_01300"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HXW09226.1"
                     /translation="MQTITRIAELREVVTEWRLGRESIAFVPTMGNLHMGHASLMAAA
                     HLHGRRVISSIFVNPLQFGPSEDFSAYPRTPDDDGSLLREYGVDALFHPTVEEMYPQG
                     SVGSTVIDVPGLTDVLCGAFRPGHFQGVATVVVKLLCLVQPDVAIFGEKDYQQLTVIR
                     RVVQDLNVPVRIVGAPTVRAEDGLALSSRNRYLSAEERAAAPAIHRALDRARRRLEAG
                     DADFAAIEAEGLDAISAAGIKPDYFEVRTADRLDRPGEDDSKLVVLTAGRLGKARLID
                     NLQCRAPVRR"
     gene            complement(967..1794)
                     /gene="panB"
                     /locus_tag="VD737_01305"
     CDS             complement(967..1794)
                     /gene="panB"
                     /locus_tag="VD737_01305"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005095698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HXW09227.1"
                     /translation="MYTQLQLRDSARPPVNVSSLRQMKEKGEKIASLTCYDASYAALV
                     DAAGTDVVLVGDSLGMVIQGHDTTVPVTLDNVIYHCRAVSKGLFRPFLMADMPFMTYT
                     SREQALQNAVRLMQEGGAKMVKLEGGAGQAEIVEFLASHDIAVCAHLGLRPQSVHKTG
                     GFRVQGREEAAAEQMKRDAKLLQDAGADIVLLECIPSALGREITEELHVPVIGIGAGP
                     HTDGQILVLYDVLDITPGRKPRFVQNFQQGRDSPLAALQAYNEAVRSGAYPAAEHCF"
     gene            complement(1871..2584)
                     /locus_tag="VD737_01310"
     CDS             complement(1871..2584)
                     /locus_tag="VD737_01310"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010158266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HXW09228.1"
                     /translation="MTATTTERHEARVDDQEETVSTVETPHRYVVVEGPIGVGKTSLV
                     RRLGKSFGCELVLEQDAENPFLERFYRDPRSAAFQTQLYFLFQRARQLQGLRQADLFE
                     QVRVADYMLDKDRLFARLTLDDQEYALYEQVYERLAIDAPAPDLVVYLQAPVDVLLDR
                     IARRGIEYEQQIERRYLERLNDAYARFFLEFDAAPLLIVNAADIDPVGSDADYQGLLA
                     EVVRPRKGRHYFNPLKSLL"
     gene            complement(2581..3120)
                     /gene="folK"
                     /locus_tag="VD737_01315"
     CDS             complement(2581..3120)
                     /gene="folK"
                     /locus_tag="VD737_01315"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HXW09229.1"
                     /translation="MTGTERADVAQDEPARAAWIPAYVALGSNLDDPGQQVERAFDAL
                     ASLPRCRLVLRSACYRSRPLGPVAQPDFVNAVAGLLTQLDAATLLQELKSLESTLGRA
                     APIVRWGPRRIDLDLLVHGSTRRQEPKLQVPHPGIAGRAFVLAPLCDVSPDLLVPGVG
                     RVRDLLARLDAGGLERIDR"
     gene            complement(3117..4454)
                     /gene="pcnB"
                     /locus_tag="VD737_01320"
     CDS             complement(3117..4454)
                     /gene="pcnB"
                     /locus_tag="VD737_01320"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HXW09230.1"
                     /translation="MPRSEHSISRANISPAALKVLYRLKDSGHQAFLVGGAVRDLLLE
                     HQPKDFDIATDARPEEVRRIFRNSRLIGRRFHLAHVRFGHEVIEVATFRAAHTEIDED
                     HGVDEVEHRVLDEHGRILRDNLYGTIEEDVWRRDFTANALYYSIEDYSIWDYVGGVED
                     ARNRVLRLIGDPATRYREDPVRMLRAARFAAKLGFTLHPDTEQPLGELAYMLDRMPPA
                     RLFDESMKLLLTGHAANSFEWLWRLDLLPHLMPDVADALEAAPESAGARLVEYGLEGT
                     DDRVRDDKSVTPTFLFAVLLWPAILQQLGAPDGPLPDDPQRLLDACDRVIGRQLKRIA
                     VPKRFSLPMRELVMLQTRFERRSGRRALRLLDHPRFRAAYDFLLLRAEAGEVPQELAD
                     WWTDIQEQPAAERVAMVEARPEPERATPGETPERRRRRRPRRRRRPPGPANAS"
     gene            4635..4710
                     /locus_tag="VD737_01325"
     tRNA            4635..4710
                     /locus_tag="VD737_01325"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4669..4671,aa:Glu,seq:ctc)
     gene            complement(4728..5318)
                     /locus_tag="VD737_01330"
     CDS             complement(4728..5318)
                     /locus_tag="VD737_01330"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HXW09231.1"
                     /translation="MQKIYAVAAVVALACTFAGATVARAAEEAYTIDTNHTHATFAFQ
                     HLGFSTFHGKVPAKGGTVVIDREARTGRVAVEFDPNAVATGVPKFDEHLRSADFFEVA
                     KHPTASFKSSRITFDEKGAPKTVIGDLTIKGITKPVTLQVTSFHCGDHPMAKVPACGA
                     NATASIKRSDYDMSFALPAVPDEIGLEIEIEAMKKK"
BASE COUNT          825 a         1847 c         1858 g          877 t
ORIGIN      
        1 agccgcgcgc cagaccgacg cgccagccac tagccactag ccactagcca ctggccactc
       61 ctcatcgccg caccggcgcc ctgcactgca ggttgtcgat cagccgcgcc ttgccgaggc
      121 ggccggcggt cagcacgacg agcttcgagt cgtcctcgcc gggccggtcg agccggtcgg
      181 cggtgcgcac ctcgaagtag tcgggcttga tacccgcggc actgatcgcg tcgaggcctt
      241 cggcttcgat cgcggcaaag tctgcgtcgc ctgcctcgag ccgacgccgg gcccggtcga
      301 gcgcccgatg gatggccggc gccgccgcac gctcctcggc actgaggtag cggttgcgcg
      361 aactcaacgc gagcccgtcc tcggcccgca cggtcggtgc gccgacgatc cgcaccggta
      421 cgttcaggtc ctgcacgacg cgccggatca ccgtgagctg ctggtagtcc ttctcaccga
      481 agatcgcgac gtcgggctgc accaggcaca gcagcttcac caccaccgtc gcgacgccct
      541 ggaagtgccc gggccggaag gcgccacaga ggacgtccgt gagtccgggc acgtcgatca
      601 cggtgctgcc gacgctgccc tgcgggtaca tctcttcgac ggtcgggtgg aacagcgcgt
      661 cgacgccgta ttcccgcagc aggctgccgt cgtcgtccgg agtgcgcggg taggcgctga
      721 aatcctcgct cgggccaaac tgcagcgggt tgacgaagat gctcgagatc acgcgccggc
      781 cgtgcaggtg cgcggccgcc atcaggctcg cgtgccccat gtgcaggttg cccatggtcg
      841 ggacgaacgc gatgctctcg cgaccgaggc gccactccgt gaccacctcg cgaagttccg
      901 cgatccgcgt gattgtctgc atcgtggatg ccgctcggca gtggtccaac gtggccgtcg
      961 tgatcgtcag aaacagtgct cggccgccgg ataggcgccg gacctcacgg cctcgttgta
     1021 ggcctgcagc gcggccagcg gactgtcgcg gccctgctgg aagttctgca cgaagcgcgg
     1081 cttgcgtccc ggcgtgatgt cgagcacgtc gtagagcacc aggatctgcc cgtcggtgtg
     1141 cggcccggcg ccgatgccga tcacgggcac gtgcagttcc tcggtgatct cgcggccgag
     1201 cgccgagggg atgcactcga gcagcacgat gtcggcgccc gcgtcctgca gcagcttcgc
     1261 gtcgcgcttc atctgctcgg cggcggcttc ctcgcgaccc tgcacgcgga agccgcccgt
     1321 cttgtgcacc gactgcggcc gcagcccgag gtgcgcgcag accgcgatgt cgtggctcgc
     1381 gaggaactcg acgatctccg cctgtcccgc accgccctcg agcttgacca tcttcgcgcc
     1441 gccttcctgc atcagccgga cggcgttctg cagcgcctgc tcgcgcgacg tgtaggtcat
     1501 gaacggcatg tccgccatca ggaacggccg gaacaggcct ttcgacacgg cacggcagtg
     1561 gtagatcacg ttgtcgagcg tgaccggcac cgtggtgtcg tgaccctgga tcaccatgcc
     1621 gagtgaatcg ccaacgagca ccacgtcggt cccggcggcg tcgacgagcg cggcgtagct
     1681 cgcgtcgtaa caggtgaggg aagcgatctt ctcgcccttc tccttcatct ggcgcagcga
     1741 ggagacgttg acgggcgggc gcgcggaatc gcgcagctgc agttgggtgt acatggtgga
     1801 tcgtgcctag cgcgagggcg caaggatgca gcagcggccg tgaaactaca acgccggcag
     1861 gcgccggctg ttacagcaag ctcttgagcg gattgaagta atgacggccc ttgcgcggcc
     1921 ggaccacttc ggcgagcagg ccctggtaat cggcgtcgct gccgaccggg tcgatgtcgg
     1981 ccgcgttgac gatcagcagc ggtgccgcgt cgaactcgag gaagaacctc gcgtacgcgt
     2041 cgttgagtcg ctcgaggtaa cgccgctcga tctgctgctc gtattcgatg ccgcgccgcg
     2101 cgatccggtc cagcagcacg tctaccggcg cctgcaggta gacgaccagg tccggcgccg
     2161 gcgcgtcgat cgcgagccgc tcgtacacct gctcgtacag cgcgtactcc tggtcgtcga
     2221 gggtcaggcg cgcaaacagc cggtccttgt cgagcatgta gtcggcgacg cggacctgct
     2281 cgaaaaggtc ggcctggcgc aggccctgca gctggcgcgc gcgctggaac aggaaataga
     2341 gctgtgtctg gaacgccgcg ctgcggggat cgcggtagaa gcgctcgagg aacgggttct
     2401 ccgcgtcctg ctcgagcacc agctcgcagc cgaagctctt gccgagccgt cgcaccaggc
     2461 tcgtcttgcc gacgccgatc ggcccctcga ccacgacgta tcgatgcggc gtctcgaccg
     2521 tgctgaccgt ctcttcctgg tcgtccacgc gagcctcgtg ccgttcggtc gtcgtcgccg
     2581 tcatcgatcg attcgctcca gtccacccgc gtccaggcgc gccagcaggt cgcgcactct
     2641 accaacgccc ggcacgagca ggtccggact gacgtcgcag agtggcgcca gcacgaatgc
     2701 gcgcccggcg atgcccgggt gtggcacttg caacttcggc tcctgccgcc gcgtcgagcc
     2761 gtggaccagc aggtcgaggt cgatgcggcg ggggccccag cggacgatcg gcgcggcccg
     2821 gccgagcgtg gactcgagcg acttcagctc ctgcagcagc gtcgccgcgt cgagctgcgt
     2881 gagcagtccc gccaccgcgt tgacgaagtc cggttgcgcg accggcccga gcggccgcga
     2941 gcgatagcac gcggagcgca gcaccaggcg acaccggggc aggcttgcca gcgcgtcgaa
     3001 cgcacgctcg acctgctgcc cgggatcgtc gaggttgctg ccgagcgcga cgtacgcggg
     3061 aatccaggcc gcgcgcgccg gctcgtcctg cgcgacgtcg gcgcgttccg tcccggtcac
     3121 gaagcgttgg ccggacctgg gggccgccgt cgccgacgcg gccgacgacg tcgccgacgt
     3181 tcgggcgtct ctccgggggt cgcgcgttcc ggttccggtc gcgcctcgac catggccacg
     3241 cgctccgcgg ccggctgttc ctggatgtcg gtccaccagt cggcgagctc ctgcgggacc
     3301 tcgccggcct cggcgcgcag cagcaggaag tcgtacgcgg cgcggaatcg cgggtgatcg
     3361 agcaggcgca aggcgcggcg gcccgagcgg cgttcgaacc gcgtctgcag catgaccagc
     3421 tcgcgcatcg gcagcgagaa ccgtttcgga accgcgatgc gtttcaactg gcggccgatc
     3481 accctgtcac aggcgtcgag cagccgctgt ggatcgtccg gaagcggccc gtcgggcgcg
     3541 ccgagctgct gcaggattgc gggccacagc agcaccgcga acaggaacgt cggcgtgacc
     3601 gacttgtcgt cgcggacgcg atcgtcggtg ccttcgaggc cgtactccac gagtcgcgcg
     3661 cccgccgact ccggggccgc ctcgagcgcg tcggcgacgt ccggcatcag gtgcggcagc
     3721 aggtcgagtc gccacagcca ctcgaacgag ttggccgcgt gaccggtcag cagcagcttc
     3781 atcgactcgt cgaacagcct cgcgggcggc atgcggtcga gcatgtaggc gagctcgccg
     3841 agcggctgct cggtgtcggg atgcagcgtg aagccgagct tcgccgcgaa tcgcgccgct
     3901 cgcagcatgc gcacggggtc ctcgcggtag cgcgtcgccg ggtcgccgat caggcgcagc
     3961 acgcgattgc gtgcgtcctc gacgccgccg acgtagtccc agatcgagta gtcctcgatg
     4021 ctgtagtaga gcgcgttggc ggtgaagtcg cggcgccaga cgtcctcttc gatcgtgccg
     4081 tagaggttgt cgcgcaggat gcggccgtgc tcgtcgagca cgcggtgctc cacctcgtcg
     4141 accccgtgat cctcgtcgat ctcggtgtgc gcggcgcgga aggtcgcgac ctcgatgacc
     4201 tcgtggccga accgcacgtg cgccaggtga aaacgccggc cgatcagccg ggagttgcga
     4261 aagatgcgcc gcacctcctc gggacgtgcg tcggtcgcga tgtcgaaatc cttcggctgg
     4321 tgctccagca gcaggtcgcg taccgcgccg cccaccagga acgcctggtg gccggagtcc
     4381 ttgagtcgat acaggacctt gagcgctgca ggcgaaatat tcgcgcgcga aatcgagtgc
     4441 tcggaccggg gcacgaccgt gggcggcggc ggaccggcgg aaggcgcaac ttcggccgcg
     4501 gacttgtcgg cagagcgcga ccggaaaagt ttagagaacg gaaacaagga ttcagccacc
     4561 gctggaccgg cgaaaacggt cgagtataat acgcgccctc tcaggaaccg gtgagtcctt
     4621 gccggtccgg acacgctccc atcgtctaga ggcccaggac acagccctct caaggctggt
     4681 acgagggttc gaatccctct gggagcgcca actcgtcccc gccggggcta tttcttcttc
     4741 atcgcctcga tttctatctc gagtccgatc tcgtccggca ccgccggcag cgcgaacgac
     4801 atgtcgtagt ccgagcgctt gatcgacgcg gtcgcgttgg ctccgcacgc gggaaccttc
     4861 gccatcgggt gatcgccgca atggaacgag gtgacctgca gcgtcaccgg cttggtgatg
     4921 cccttgatcg tgaggtcgcc gatcaccgtt ttcggcgcgc ccttctcgtc gaaggtaatg
     4981 cggctcgact tgaaactggc ggtcgggtgc ttcgcgacct cgaagaagtc ggcgctccgc
     5041 aggtgctcgt cgaacttcgg cacgccggtg gcgacggcgt tcggatcgaa ctcgacggcg
     5101 acgcggcccg tcctggcctc gcggtcgatg acgaccgtgc cgcccttggc ggggaccttg
     5161 ccgtgaaagg tcgagaagcc gagatgctgg aacgcgaacg tcgcgtgcgt gtgattcgtg
     5221 tcgatcgtgt aggcctcttc ggcggcccgg gcgacggtcg cccccgcgaa ggtgcacgcg
     5281 agcgctacga cggcggcaac ggcgtaaatc ttctgcacgg tatggctcct tgcgtccaac
     5341 gattgttcct gaactcgcac ctgccggtaa tcgacccgcg gcgacgcact tcgttccgct
     5401 cgcgtgt
//
LOCUS       DASPFR010000055         6983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1484314, whole genome shotgun sequence.
ACCESSION   DASPFR010000055 DASPFR010000000
VERSION     DASPFR010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6983
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484314"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VD737_01335"
     CDS             complement(<1..276)
                     /locus_tag="VD737_01335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXW09232.1"
                     /translation="MPVYNGEAFLREAIESVLAQTHRNFELVAVDDGSTDGSPAILAE
                     FAAADPRVRVITQRNCGGARARNRALRAARAEWVINLDCDDAFLPNRI"
     gene            complement(324..1907)
                     /locus_tag="VD737_01340"
     CDS             complement(324..1907)
                     /locus_tag="VD737_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXW09233.1"
                     /translation="MIDPPRKLLIIGVDAGSPELFRRWCSTGDLPNLGRLLADGIARR
                     VENPRGMEAGAVWPVFHTGLLPGRQPMYDGRRNFDPATYAERWYGPDETMPTVWRQLS
                     DAGLRCVLIDPPYTLVDPRINGVTVMDWGTHVPANGQRFEFRTHPANVAAEILALVGP
                     DPAGGVMCDRMSPESVDEYRRFRDLYLTRIDGKAKLARHLLTNEDWNVALVTSSDLHC
                     AGHHLWHINDRRHPQYSAQVEAELGEPLKDCYRAFDRSLGTILDAVDAGTTVMVFGSH
                     GMGPSFSGTGLLDRILLRLDKGTVRVARHSPKARLRALWHRVPADLRGRLRPLRRPFR
                     GALHPPKFLGDHANRRFFEVYANNASGGVRLNIKGRERSGTVDPADADDLSRFLATEL
                     GKVVNADTGEPVVKDVTVTRRVYSGAYLDKLPDLLVTWNRNAPIERVRSDTIGQLSRE
                     YLDNRTGDHTPDGICIVHGPGVNGRGEAPAIRTADLAPSIARFFGVELHEHDGAPFDL
                     VSRKPFEREPADVESRSGA"
     gene            2229..3878
                     /locus_tag="VD737_01345"
     CDS             2229..3878
                     /locus_tag="VD737_01345"
                     /inference="COORDINATES: protein motif:HMM:NF015530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09234.1"
                     /translation="MTMRTAVLVGIATTATVLAGPANADEVKMQQATTVPARPEIYAP
                     FRLTADLSGLSENERRMLGLFIDASVIMDDLFWRQAYGNRDALLAGLAKDPRQQEFAR
                     INYGPWDRLADHAPFVAGVGPKPLGAQFYPADMTKEEFEKANLPQSRSEYTLLRRDAK
                     GALEVVPYHQAYADGVTRAAALLEQAAGLAEDAGLKAYLQARATALRTDDYRASDRAW
                     LDMKNNRIDVVIGPIETYEDRLFGYKAAYEAYVLVKDMDWSRRLARYAAMLPELQRGL
                     PVPDAYKAEKPGTDSDLNAYDVLYYAGDCNAGSKTIAINLPNDEQVQLEKGTRRLQLK
                     NAMRAKFEKIMLPIADELIVPEQRKHVTFDAFFGDVMFHEVAHGLGIKNTIDGKGTVR
                     DALKELGSGIEEGKADVLGLYMITKLHEKGEVTGSLEDYYVTFLTGIFRSVRFGAGSA
                     HGQANMLRFNYFADRGAFTRDAASGRYRVDMPKMRRAMDELSALILKLQGDGDYAGVQ
                     KLTGDLGSIGPQLESDLARLAARSIPVDVVFEQGKSVLGLK"
     gene            3998..4924
                     /locus_tag="VD737_01350"
     CDS             3998..4924
                     /locus_tag="VD737_01350"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HXW09235.1"
                     /translation="MNAVHRVIEPRPRDLGDFQVRRLLPAPGLQTVGPFIFWDHMGPA
                     DFGPDQGVNVRPHPHIGLATVTFLYEGAFMHRDSLGTAQVIRPGDVNWMIAGRGIAHS
                     ERTPPEVRGPDGHTRAHGIQTWVALPREHEEIEPSFEHHPAATLPRLELDGVRLTVIA
                     GTAFGRRSPVNVLSPTLYVDAQFAEAGAGCTLDDEHDQRAVYVAQGSVDVEGEVYREG
                     QLVVLEPADAVRIVATAADTRVMMAGGAPLDGPRHIWWNFVSSSQERIELAKRQWREQ
                     RVSPVPGDPERIPLPDESLPGTPPAPAAPPLS"
     gene            complement(4936..5475)
                     /locus_tag="VD737_01355"
     CDS             complement(4936..5475)
                     /locus_tag="VD737_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09236.1"
                     /translation="MPRSHEIRFQRAARVRVVAALLATPALLGPLATEAAATRTPTGG
                     GGSVVLEPVPSPERRTWRRLVYTCTSPGLVVFSDRPCGPLPIVQEIKGHTPLGSPGAS
                     GAVASVVPPKPAASTRPAPSEPALDAGETGREATCRRLQDAVRALDDRMRAGYSAREA
                     ATLWSRWREAKQRLREADC"
     gene            complement(5577..5885)
                     /locus_tag="VD737_01360"
     CDS             complement(5577..5885)
                     /locus_tag="VD737_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09237.1"
                     /translation="MTDTQDEAHPFEEAFGRAVDLGNQIADNDDKADLWDIADGLLAG
                     AVQYWLYTRQPCGDPRCEDCLAVGTAEARMAELRRLVEQFSEESQYFHSPTDSNVGRA
                     "
     gene            complement(5993..6259)
                     /locus_tag="VD737_01365"
     CDS             complement(5993..6259)
                     /locus_tag="VD737_01365"
                     /inference="COORDINATES: protein motif:HMM:NF025715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HXW09238.1"
                     /translation="MSDLQHTLAELRRELSAAGPLAPAERALLESTIHDIQRLLARDS
                     GPVTEPAAAPGEALEGAAVRLEAEHPGVAGAVRALVDALAKAGI"
     gene            6369..>6983
                     /locus_tag="VD737_01370"
     CDS             6369..>6983
                     /locus_tag="VD737_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXW09239.1"
                     /translation="MPLRLRHHAVALAATLLAAVAQAEAPRFEVSPFAGGRIGGGFDV
                     VEDEATGDETSVDLDNGGSFGVDLGLYAHPNGFYELLYSTQETSLDADDPAVDGVDVR
                     IDYLHVGGTALFPQDRFYVPYLSFTLGATFLEPTSGGYDSETKFSASLGGGFRFPVSE
                     HFQVLLGLRGYLTLIDSDTDLFCVSDAEQAGCLVRSSGSTFFQTE"
BASE COUNT         1088 a         2383 c         2432 g         1080 t
ORIGIN      
        1 gatccggttc ggcagaaagg cgtcgtcgca gtcgaggttg atcacccatt cggcgcgcgc
       61 cgcccgcagc gcgcggttgc gcgcccgggc gccaccgcaa ttgcgctggg tgatcaccct
      121 cacccgaggg tccgctgcgg cgaactccgc gagaatcgcc ggcgaaccgt cggtcgagcc
      181 gtcgtcgacc gccaccagct cgaagttccg gtgcgtctgc gcgaggacgc tctcgatggc
      241 ttcacgaagg aatgcctcgc cgttgtagac cggcatgatc accgagacga acggtcggcc
      301 gtcgccgtat gaagacttgc cgttcaagct cctgacctcg actcgacatc cgcaggctcc
      361 cgctcgaacg gcttcctgga cacgaggtcg aagggcgcgc cgtcgtgctc gtggagctcg
      421 actccgaaga agcgggcgat gctcggtgcg aggtctgccg tcctgatcgc gggtgcttcg
      481 ccgcgcccgt tgacgcctgg gccgtggacg atgcagatgc cgtcgggtgt gtggtcgccc
      541 gtgcggttgt cgaggtactc ccgtgacaac tggccgatcg tgtcggaccg caccctctcg
      601 atcggtgcat tccgattcca ggtcaccagc aggtccggca gcttgtcgag gtaggcgccg
      661 gaatacacgc gacgtgtcac cgtgacgtcc ttgacgaccg gctcgccggt gtcggcgttg
      721 acgaccttgc cgagctccgt cgcgaggaag cgcgacaggt cgtcggcgtc ggccgggtcc
      781 acggtaccgc tgcgttcgcg tcccttgatg ttcagccgga cgccgccgga cgcgttgttt
      841 gcgtacacct cgaaaaagcg ccggttggcg tggtcaccga ggaacttggg cggatgcagc
      901 gcgccgcgga acggccgccg caatggcctc aggcggccgc gcaggtccgc cggcacgcgg
      961 tgccacagcg cgcggagcct cgctttgggc gagtgccgcg ccacccgcac cgtgcccttg
     1021 tcgagcctca gcagaatccg atcgagcagc ccggtgccgc tgaacgaagg ccccatgccg
     1081 tggctgccga acaccatgac ggtcgtgccc gcgtcgacgg catcgaggat cgtaccgagg
     1141 ctccggtcga acgcccggta gcagtctttc agcggctccc cgagttccgc ctcgacctgc
     1201 gcggaatact gcgggtgccg tcgatcgttg atgtgccaca ggtggtgacc cgcgcagtgc
     1261 aggtcggagc tcgtgaccag cgcgacgttc cagtcctcgt tcgtcagaag atggcgcgcg
     1321 agcttcgcct tgccgtcgat ccgcgtcaga tagaggtcgc gaaagcgccg gtattcgtcg
     1381 acgctctcgg gcgacatgcg atcgcacatg accccgcccg ccggatcggg accgaccagc
     1441 gccaggatct cggccgcgac gttcgccggg tgcgtcctga actcgaatct ctggccgttc
     1501 gcgggcacgt gcgtacccca gtccatgacg gtcacgccgt tgatccgcgg atccacgagc
     1561 gtgtacggcg gatcgatcag cacgcaccgc agcccggcgt ccgacagttg tcgccagacc
     1621 gtcggcatcg tctcgtcggg gccgtaccag cgctcggcgt aggtcgccgg atcgaagttc
     1681 cgccgcccgt cgtacatcgg ctgccggccc ggcagcagcc ccgtgtggaa caccggccac
     1741 acggcaccgg cttccatgcc gcgtggattc tccacgcgac gggcgattcc gtccgccagc
     1801 aggcgtccca ggttgggaag atcgccggtc gagcaccagc gacggaacag ttccgggctg
     1861 ccggcatcga cgccgatgat cagaagcttg cgcgggggat cgatcaagtg ggggttcctc
     1921 gggtatgcga gatgcggcgc cgcggcggga tcttggcccg cgcctgggat gccacacgga
     1981 tgatcgccgc gtcgcgtcga tctggccgac gccggccacg aaggcctcca tcgttcgcga
     2041 tgcaacgtcc gcgggccgta gggggcatac gccagttcct gtcggctgaa gcccgacgca
     2101 cggcagcggg cgggcggcgg agcgacgcca cccgccgcgc cggcgggccg cgacgttgat
     2161 tatgttcaac tggggacgcc ggggcccggt gcggtaatct gtcgcgagat ttcgaggaga
     2221 cgatcgtcat gacgatgcga acggcggtgc ttgtgggcat cgcgaccacc gcgaccgtgc
     2281 tggcaggccc ggcaaacgcc gacgaggtga agatgcagca ggcgacgacc gtgcccgcac
     2341 gcccggaaat ctacgcgccg ttccggctga ccgcggacct ctccggcctg agcgagaacg
     2401 agcgtcgcat gctcgggctg ttcatcgacg cgagcgtcat catggacgac ctgttctggc
     2461 gccaggccta cgggaaccgt gacgcgctgc tcgcaggact cgcgaaggac ccgcggcaac
     2521 aggaattcgc caggatcaac tacggaccct gggaccggct cgccgatcac gcgccgttcg
     2581 tggccggagt cggccccaag ccgctcggtg cgcagttcta tccggcggac atgaccaagg
     2641 aggaattcga gaaggctaac ctgccgcagt cgcgcagcga atacacgctg ctgcgccgcg
     2701 atgccaaggg cgcgctcgag gtggtcccgt accaccaggc ctacgcggac ggcgtgacgc
     2761 gcgcggccgc gctgctggag caggccgccg gcctcgccga ggatgccggc ctgaaggcgt
     2821 atctgcaggc gcgcgcaacg gcgctgcgca ccgacgacta ccgcgcgagc gatcgcgcgt
     2881 ggctcgacat gaagaacaac cgcatcgacg tcgtgatcgg tccgatcgag acgtacgagg
     2941 accggttgtt cgggtacaag gccgcctacg aagcctacgt gctggtcaag gacatggact
     3001 ggagccgccg gctcgcgcgt tatgccgcga tgctgccgga actgcagcgc ggcctgccgg
     3061 tgccggacgc gtacaaggcc gagaagccgg gtaccgactc cgacctgaac gcctacgacg
     3121 tgctctatta cgccggcgac tgcaacgccg gctccaagac catcgcgatc aacctcccca
     3181 acgacgagca ggtgcagctc gagaagggca cgcgccggct gcagctcaag aacgcgatgc
     3241 gcgccaagtt cgagaagatc atgctgccga tcgccgacga gctgatcgtg cccgagcagc
     3301 gcaagcacgt cacgttcgac gcgttcttcg gcgacgtgat gttccacgag gtcgcgcacg
     3361 gcctcggcat caagaacacg atcgacggca aggggacggt gcgcgacgcg ctcaaggagc
     3421 tcggcagcgg catcgaggaa ggcaaggctg acgtgctcgg gctctacatg atcacgaagc
     3481 tgcacgagaa gggtgaggtg acgggctcgc tcgaggacta ctacgtgacg ttcctgaccg
     3541 gcatcttccg gtcggtgcgc ttcggcgcgg gctccgcgca cggccaggcg aacatgctgc
     3601 gcttcaacta cttcgcggac cgcggcgcgt tcacgcgcga cgcggcgagc ggtcgctacc
     3661 gcgtcgacat gccgaagatg cgccgggcca tggacgaact ctcggcgctg atcctcaagc
     3721 tccagggcga cggcgactac gcgggcgtcc agaagctgac cggcgacctc ggctcgatcg
     3781 gtccgcagct cgagtccgac ctcgcgcgcc tcgccgcgcg cagcatcccg gtcgacgtcg
     3841 tgttcgagca gggcaagagc gtgctcgggc tcaagtaaaa ccgaacaggc agtgtctagt
     3901 gtctggtgtc tagcggttag taggaggccg atggcgtcgg tgcgagcgac gctgcctcct
     3961 actagacact agacactaac cactagacac tgactcgatg aacgcagttc atcgagtgat
     4021 cgagccgcgt ccgcgcgatc tcggcgactt ccaggtccgg cgcctgctgc ctgcgccggg
     4081 gttgcagacc gtgggcccgt tcatcttctg ggaccacatg ggcccggcgg acttcggtcc
     4141 ggaccagggc gtcaacgtgc ggccgcaccc gcacatcggc ctcgccaccg tcacgttcct
     4201 ctacgaaggc gcgttcatgc accgcgacag cctgggcacc gcgcaggtga tccgcccggg
     4261 cgacgtcaac tggatgattg ccggtcgcgg catcgcgcac tccgaacgga cacctccgga
     4321 ggttcgcgga cccgacgggc acacgcgcgc gcacggcatc cagacctggg ttgcgctgcc
     4381 gcgggagcac gaggagatcg agccctcgtt cgagcatcac cccgccgcga cgctgccgcg
     4441 cctggagctg gacggcgtgc gactcaccgt gatcgccggc acggcgttcg gccgtcgctc
     4501 tccggtgaac gtgctgtcgc ctaccctgta cgtggacgcg cagtttgccg aggccggtgc
     4561 cggatgcacg ctcgacgacg aacacgatca gcgtgcggtc tacgtcgcgc agggaagcgt
     4621 ggacgtggag ggcgaggtgt atcgcgaagg ccaactcgtg gtgctcgagc ctgcggacgc
     4681 ggtgcgcatc gtggcgaccg cggccgacac ccgcgtgatg atggcgggcg gcgccccgct
     4741 cgacggcccg cgccatatct ggtggaactt cgtctcgagc tcgcaggaac gcatcgagct
     4801 cgcgaagcgg cagtggcggg aacagcgcgt ttcaccggtg ccgggcgacc cggagcgcat
     4861 cccgttgccc gacgagtcgc tgcccggcac gccacctgcg cccgcggcgc cgccgctcag
     4921 ctgacgccgc cgccctcagc agtcggcctc gcgcagacgc tgcttggcct cgcgccagcg
     4981 gctccagagc gtcgccgctt cgcgtgcgga atagccggcg cgcatgcggt cgtcgagcgc
     5041 ccgcacggcg tcctgcaggc ggcgacaggt ggcctcgcgc cccgtctcgc ccgcgtcgag
     5101 ggccggctcg gagggcgccg gtcgggtgga ggccgcgggc ttcggcggca ccaccgaggc
     5161 gacggcgccg ctcgcgcccg gtgacccgag cggcgtgtgt cccttgattt cctggacgat
     5221 cggcaggggc ccacacgggc ggtcggagaa gacgaccagg ccgggcgacg tgcaggtgta
     5281 gacgaggcgc cgccaggtgc ggcgctcagg tgacggcacg ggttcgagca cgacgctgcc
     5341 gccgcccccg gtcggcgtcc gggtggccgc ggcttccgtt gccagcggcc caagcagcgc
     5401 cggtgtggcc agcaaggccg cgacgacgcg gaccctggcg gcgcgctgga agcggatctc
     5461 gtggctgcgc ggcatggcga cgacccccac gttggcgagt ctcgatgctc gcgcgcggcc
     5521 gtggcgtcgt cagcccgagt ttgtgcgcgg tacgcgccga ttcgtccgtg aggcgctcag
     5581 gcgcggccga cgttcgaatc ggtcggcgaa tggaagtact ggctttcttc ggagaactgc
     5641 tccaccaggc gtcgcagttc ggccatgcgc gcctcggccg tgccgaccgc caggcagtcc
     5701 tcgcagcggg gatcgccgca gggctggcgc gtgtagagcc agtactgcac ggcgcctgcc
     5761 agcaggccgt cggcgatgtc ccagaggtcg gccttgtcgt cgttgtcggc gatctggttg
     5821 ccgaggtcga cggcgcgacc gaaggcttct tcgaacgggt gggcttcgtc ttgggtgtcg
     5881 gtcacgatcg ggagtgatgc gatgtgagga atccgcgatc atagcaaaac gcgtcgcgca
     5941 gcgccgcggc gcggcgcgcg gaaatcactg ggaaaccagc aatttcagcg gctcagatcc
     6001 ccgccttggc gagcgcgtcg accagtgcgc gcacggcccc ggccacgccg gggtgttccg
     6061 cctccagacg cacggccgcc ccctcgagcg cctcgcccgg ggctgcggcc ggttcggtca
     6121 cggggcccga gtctcgcgcg agcaaccgct ggatgtcgtg gatggtcgac tcgagcagcg
     6181 cgcgttctgc cggcgccagc ggtccggccg ccgacagttc gcgacgcagt tcggcaagag
     6241 tgtgctgcag atcgctcatg gaccctccac cgtgcggacc tcgccagcag tgtagcagcc
     6301 gtcctgcgat gcgacggcgt cacgaggctg tttacaatgc gggcccccac gtctgccgga
     6361 agccccgcat gccgctacgt cttcgccatc atgccgtcgc gctcgctgcg acgctgctcg
     6421 cggccgttgc ccaggccgaa gcgccccggt tcgaagtgag cccgttcgcc ggcggccgca
     6481 tcggaggagg tttcgacgtg gtcgaggacg aagcgaccgg cgacgagacc tccgtcgatc
     6541 tcgacaacgg cggcagcttc ggcgtggacc tgggcctcta cgcgcatccg aacggcttct
     6601 acgaactgct ctattcgacg caggagacga gcctcgatgc agacgatccc gcggtggacg
     6661 gggtcgacgt gcggatcgac tacctgcacg tgggcggcac ggcgctgttt ccgcaggatc
     6721 gcttctacgt gccgtacctg tcgttcacgc tcggcgcgac tttcctggag ccgacgagcg
     6781 gcggctacga ctcggagacc aagttctccg ccagcctggg cggtggcttc cggttccccg
     6841 tcagcgagca tttccaggta ctgctcggac tgcgcgggta cctgacgctg atcgactccg
     6901 acacggacct gttctgcgtg agcgatgccg agcaggcggg ttgcctcgtc cggtcctcgg
     6961 gcagcacctt cttccagacc gag
//
LOCUS       DASPFR010000056         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_873558, whole genome shotgun sequence.
ACCESSION   DASPFR010000056 DASPFR010000000
VERSION     DASPFR010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873558"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="VD737_01375"
     CDS             complement(<1..1191)
                     /locus_tag="VD737_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09240.1"
                     /translation="MFTRRTFLKYTGATTLTLFATGIRGLRRAIAAALPGGTLDPASI
                     PKFVTPLLVPPVMPRAGTIRRRAGKNVDYYEISVRQFSQQILPAGLPPTTVWGYGPIT
                     GAGTRNPLLIHNAPSLTIEADWERPVRVKWVNDLKDAGGHHLSHLLPVDPTLHWANPA
                     GGISGRDTRPTVASSPEPYRGPVPMVTHVHGAVAVGDESDGYAEAWYLPDVTDLPAGY
                     ATHGTWYEFFADKALRKYDAAWGPGFATYEYPNENRASTLWYHDHALGLTRLNVYAGP
                     AGFFIVRGGPAGDSAVLDVRSGTEATLPGPAPKPGEGFPARRTYYEIPVAIQDRSFNV
                     DGSLFYPDSRRFFDDLVGPHVGTEPGVLREHGHGQRQHLAVPDRRAAPLPLPLPERLP
                     VALPD"
     gene            1531..>2582
                     /locus_tag="VD737_01380"
     CDS             1531..>2582
                     /locus_tag="VD737_01380"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HXW09241.1"
                     /translation="MSVPAGSKPGQRVRATPKGRQVDPRAREEVRALLGDSPRRRDLL
                     IEHLHRIQDRYGHLSAAHLAALAEEMRMAMAEVYEVATFYHHFDVIKEGETAPPSITV
                     RVCDSIACELAGGGELYASLASLLGPGVRVLPTSCVGRCETAPVAVVGQNPVPNATPE
                     NVTRLVEAGAVVHPRRTEPAAQPHLDVVTPGHIDYAAYRATGGYALAADCVTGRRSAA
                     DVIAAMEHSGLRGLGGAGFPAGRKWKIVAAEPAPRLMAINLDEGEPGTFKDRYYLERD
                     PHRFIEGAVVAASVVGIDEVYIYLRDEYHGCRQVLQQELAALNAAPPCALPTFHLRRG
                     AGAYICGEESAMIESI"
BASE COUNT          423 a          864 c          888 g          407 t
ORIGIN      
        1 atcaggaagc gcgactggca gccgttcagg aagcggaagc ggtagcggcg ctgctcgacg
       61 atctggaacg gccaggtgtt gccgttgacc atgaccgtgt tcccgaagaa ctccgggttc
      121 cgtgcccacg tgcggtccga cgaggtcgtc gaaaaaccgc cgcgagtccg gatagaacag
      181 cgagccgtcg acgttgaacg agcgatcctg gatcgcaacc gggatctcgt aataggtccg
      241 gcgcgctgga aagccttcgc ccggcttcgg tgccggtccg ggtaacgtgg cctcggtgcc
      301 ggatcggacg tcgagcacgg cggaatcgcc ggcgggtcct ccccgtacga tgaagaaccc
      361 ggccgggccg gcatagacgt taagtcgggt caggcccagc gcgtggtcgt ggtaccagag
      421 tgtcgatgcg cggttctcgt tcggatactc gtacgtcgcg aaccccggcc cccacgctgc
      481 gtcgtacttc ctcagtgcct tgtcggcaaa gaactcgtac caggtgccgt gcgtggcgta
      541 tccggccggc aggtccgtga cgtccggcag gtaccaggct tccgcataac cgtcgctctc
      601 gtcccctacg gcaaccgcac catgcacgtg tgtgaccatc ggtaccgggc cccggtaggg
      661 ctctggtgac gacgcgacgg tgggccgtgt gtcccgtccg gagatgcccc ccgcaggatt
      721 ggcccaatgc agcgtcgggt cgaccggcaa caggtgcgac aagtgatgcc cgccagcgtc
      781 cttcaaatca ttgacccact tcacacgcac cggacgctcc cagtcggctt cgatggtcag
      841 tgaaggtgcg ttgtggatca gcagcggatt gcgcgtgccg gcgccggtga tcggaccata
      901 tccccagacg gttgtcggcg gcaggccggc cggcaggatc tgctgtgaaa actgccgcac
      961 cgagatctcg tagtagtcga cgttcttgcc ggcccgacgc ctgatcgtgc cagctctcgg
     1021 catgacgggc ggcacgagca acggtgtgac gaacttgggg atggacgccg ggtcgagcgt
     1081 gccgcccggc aacgcggctg cgatcgcgcg gcgcaagcca cggatgcctg tcgcgaacag
     1141 cgtgagcgtc gtggcgccgg tgtacttgag aaacgtgcgt cgcgtgaaca tcgccgcaag
     1201 tccttctgct cgggggcacc tcggccgggc gtgctcggtt ggcgtagggt gggccggtcg
     1261 gtgtggccgc agaaatccgg aaaaaccgac ggggtgccgt gaccattgcg gacgagtgat
     1321 ctcgtccctg tcagcgtccg gacacgctgc ggcgtgccgg caccggtcat tgaagaaagg
     1381 gttggagggt agcgaagcgg cccgggcctc tcatcaccga ccagggcacc cgcaacgctt
     1441 ctggtgcctt gcctcgctac gttcggccgg gtgctagcat cggctttcca tcctcccgct
     1501 acagcgatga gcgaccgcga tccccagcgc atgtccgtcc cggcgggttc gaagcccggc
     1561 cagcgcgtcc gcgccacccc gaaagggcgt caagtcgatc cccgggcgcg cgaggaggtc
     1621 cgcgcgctgc tgggcgactc accgcgacgc cgggacctgt tgatcgaaca cctgcaccgg
     1681 atccaggacc gctacggcca cctgtcggcg gcgcacctcg ccgcgctcgc cgaagagatg
     1741 aggatggcca tggccgaggt ctacgaggtg gcgacgttct accaccactt cgacgtgatc
     1801 aaggaaggcg aaacggcgcc gccctcgatc accgtgcgcg tctgcgactc gatcgcgtgc
     1861 gagctcgcgg gaggcggcga gctctacgca tcgctggcgt ccctgctggg ccccggcgtc
     1921 cgcgtgctgc cgacgtcgtg cgtgggaagg tgcgagacgg cgcccgtcgc cgtggtcggc
     1981 cagaacccgg ttcccaacgc gacaccggag aacgtcacgc gactcgtcga ggccggcgcc
     2041 gtggtgcacc cgcgacgaac cgagcccgct gctcagccgc acctcgacgt cgtcacgccc
     2101 ggccacatcg actacgcggc gtatcgcgcg acgggcggct acgcgctcgc cgccgattgc
     2161 gtgaccggcc gccgcagcgc cgccgacgtg atcgccgcca tggaacactc gggcctgcgc
     2221 ggcctgggcg gagccggctt tccggccggg cgcaagtgga agatcgtggc ggccgaaccg
     2281 gcgccgcggt tgatggcgat caacctggac gaaggcgagc cgggcacgtt caaggaccgg
     2341 tattacctgg aacgcgatcc gcatcgcttc atcgaaggcg ccgtcgtcgc ggcaagcgtc
     2401 gtcggcatcg acgaggtcta catctacctc cgggacgagt accacggctg ccggcaggtc
     2461 ctgcagcagg aactcgccgc attgaacgca gcgccgccgt gcgcactgcc gacgttccac
     2521 ctccgacgcg gtgccggtgc gtacatctgc ggcgaagaat ccgcgatgat cgagtcgatc
     2581 ga
//
LOCUS       DASPFR010000057         3518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_263809, whole genome shotgun sequence.
ACCESSION   DASPFR010000057 DASPFR010000000
VERSION     DASPFR010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263809"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VD737_01385"
     CDS             <1..367
                     /locus_tag="VD737_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXW09242.1"
                     /translation="LEQVMINLLKNAAEAGSPPAQTSVRVYGHRGGWTVEVLDRGSGM
                     TPEVLTSALLPFYSTKPAGSGLGLTLCREIVEAHGGTLEAGNRADGPGAVVRLWLPPA
                     PAVDYGLRSSAEPESIHTP"
     gene            complement(319..567)
                     /locus_tag="VD737_01390"
     CDS             complement(319..567)
                     /locus_tag="VD737_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09243.1"
                     /translation="MLHAHRSLPQVGAQDPELRNSPIAEEEDEETDVVRQQLPGAAAC
                     WFNGTEYGQGTYVVSGSQVLRCHYGVWMDSGSADERNP"
     gene            713..2458
                     /locus_tag="VD737_01395"
     CDS             713..2458
                     /locus_tag="VD737_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXW09244.1"
                     /translation="MGTLRIKPEIRSVPIFTLEFLRSQIVGIDATFETPFGRRLMVYC
                     DYTASGRCLAFVERYLQNLQRIYANTHTEDDLTGRSMSRLLHEAERTITDAVNAGPRG
                     RIVACGTGATGAIDKLQQILGVALPPATRRTVGNHLREFLGDAQFEAFRRYQQSKQPV
                     VFVGPYEHHSNEVTWRQGLASVVEVNLAPDGGIDLGHLEHLLQAPEYRGRLRIGSFSA
                     ASNVTGMRSPAREIARLLHAHDALACFDFAASAPYVEIDMNPADDPHGDASFDAIFIS
                     PHKFLGGPGSSGVLVFNERLYCRDLPPSVGGGGTVDYVSPIDQDFIVDVEQREKAGTP
                     GVLQTLKAALAFDLKRRVGVARIEARERELLERAMARLGRNERIEILGNPDPARRIGI
                     VSFNVRDPRGRYLHPKFVTVLLNDLFGIQSRAGCSCAGPYGHRLLGIDDTTSEKYRDW
                     VRRGFQGIKPGWCRVGFHYTMDDAEAEYVIDAVECVAACGHRFLPLYAFDPATGFWSH
                     RTHVEQHEPFSVDAALDCSGCDQSPLPVETRREYYEHYLRHARTLAEGLGEPGAGASS
                     SRDEQFGELQFFNVD"
     gene            2464..>3518
                     /locus_tag="VD737_01400"
     CDS             2464..>3518
                     /locus_tag="VD737_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXW09245.1"
                     /translation="MARRHAGAMLRRATVVGVILLATACERDQGASSPHPGHGPAGDT
                     IVARIGGREFEQADADAPIAGALHELDYERYRLRRESLEAVLLRALAEPQSREKSAQV
                     LLRPPLPPRVALDGAPAASRPDGVRHPVTVTVFCNFESPHCADVQSLLADLRALHPEA
                     IVVAARDLPLPMHGLAPLAAEAARCAGRQGRYWPFHDLLFAGRRAPDRTELERVATAV
                     GVDRETFDRCLETHDTASEVGADVALAHRLRLGMVPAVFVNGRRAVAPVTIDQLLWLV
                     SAELATAEPARAVVAPASSLPVSLRATLVGEVPGLGLALLEVNGRRQVVREGERATSN
                     AILRRVDARGAELLVDGR"
BASE COUNT          507 a         1252 c         1239 g          520 t
ORIGIN      
        1 gctcgagcag gtgatgatca acctgctcaa gaacgccgcc gaggcgggct cgccgccggc
       61 ccagaccagc gtgcgggtgt acggccaccg cggcgggtgg acggtcgagg tgctcgaccg
      121 cggctccggc atgacgcccg aggtgctcac gagcgcgctg ctgccgttct attcgaccaa
      181 gccggccggc agcggcctcg ggctgacgct gtgccgcgag atcgtcgagg cgcacggtgg
      241 cacgctcgaa gccggcaacc gcgccgacgg gcccggcgcg gtcgtccggt tgtggctgcc
      301 gcccgcgccg gcggtcgact acgggttgcg ctcgtcggcc gagccggagt ccatccacac
      361 gccgtagtgg cagcgcagca cctgcgagcc gctcacgacg taagtgccct gcccgtattc
      421 ggtgccgttg aaccagcagg cggccgcgcc gggcagttgc tggcgcacga cgtccgtttc
      481 ctcgtcttct tcctcggcga tcggcgagtt acgcagctcg gggtcctgcg cgccgacctg
      541 ggggagcgat cgatgtgcgt gcaacatggc cgtactcccg aaatggggac actccgaatt
      601 atggaccgtt tagggacact cctgatttcg gctctacgaa aaagccggaa tcgcgaccgc
      661 gccccggcga ccagccacgc cgacccactg aaattggcgc caaatccgga aattggggac
      721 actccggatt aagccggaaa ttcggagtgt ccccattttt acgctggagt tcctgcgctc
      781 gcagatcgtc ggcatcgacg cgacgttcga gacgccgttc gggcggcggc tgatggtcta
      841 ctgcgactac accgcctcgg ggcgctgcct cgcgttcgtc gagcgctacc tgcagaacct
      901 gcagcgcatc tacgccaaca cgcacaccga ggacgacctg accggccgca gcatgagccg
      961 gctgctgcac gaagcggagc gcaccatcac ggacgcggtc aacgcgggcc cgcgcggccg
     1021 catcgtcgcg tgcggcacgg gcgcgaccgg ggcgatcgac aagctgcagc agatcctggg
     1081 cgtcgcgctg ccgcccgcca cgcgccgcac ggtcggaaac catctccgcg agttcctggg
     1141 cgacgcgcag ttcgaggcgt tccggcgcta ccagcagtcg aagcagccgg tcgtgttcgt
     1201 cggcccctac gagcaccaca gcaacgaggt gacgtggcga cagggcctcg cgagcgtcgt
     1261 cgaggtgaac ctcgcccctg acggcggcat cgacctcggg cacctcgaac acctgctgca
     1321 ggcgcccgag tatcgcggcc ggctgcgcat cggctcgttc tcggcggcct cgaacgtcac
     1381 gggcatgcgc tcccctgcgc gggagatcgc ccggctgctg cacgcccacg acgcgctcgc
     1441 ctgcttcgac ttcgccgcga gcgccccgta cgttgagatc gacatgaacc cggcggacga
     1501 cccgcacggc gacgcctcgt tcgacgcgat cttcatctcg ccgcacaagt tcctcggcgg
     1561 gcccggttcg agcggcgtgc tggtcttcaa cgagcggctc tactgccgcg acctgccgcc
     1621 gagcgtgggc ggcggcggca ccgtcgacta cgtgagcccg atcgaccagg acttcatcgt
     1681 cgacgtcgag cagcgcgaga aggccggcac gccgggcgtg ctgcagacgc tcaaggccgc
     1741 gctcgcgttc gacctgaagc gccgggtcgg cgtcgcgcgc atcgaggcgc gtgagcgcga
     1801 gctgctcgag cgcgcgatgg cacgcctcgg gcgcaacgag cgcatcgaga tcctcggcaa
     1861 tccggacccg gcgcggcgca tcggcatcgt ctcgttcaac gtgcgcgacc cgcgcggccg
     1921 ctacctgcac cccaagttcg tgacggtcct gctcaacgac ctgttcggca tccagtcacg
     1981 cgccggctgc tcctgcgcgg gcccgtacgg tcaccgcctg ctcggcatcg acgacacgac
     2041 ctccgagaag tatcgcgact gggtgcgcag gggcttccag ggcatcaagc cgggctggtg
     2101 ccgcgtgggc ttccactaca ccatggacga cgcggaggcc gagtacgtga tcgacgcggt
     2161 ggaatgcgtc gcggcgtgcg gccaccgctt cctgccgctg tacgcgttcg atccggcgac
     2221 gggcttctgg tcgcatcgga cgcacgtcga gcagcacgaa ccgttctccg tcgacgcggc
     2281 gctcgactgc agcgggtgcg accaatcccc gctgcccgtc gagacgcgcc gcgaatacta
     2341 cgagcactac cttcgacacg cgcggacgct ggccgaagga ctcggcgaac ccggggcggg
     2401 tgcgtcgtcg tcgcgcgacg agcagttcgg cgagttgcag ttcttcaacg tggactgaac
     2461 gccatggcgc gtcgccacgc aggtgcaatg ctgcggcgcg cgacggtcgt tggcgtgatc
     2521 ctgctcgcca cggcctgtga gcgcgatcaa ggtgcatcgt cgccgcaccc cggccacgga
     2581 ccggccggcg acacgatcgt cgcgcgcatc ggtggccgcg agttcgagca ggcggacgcc
     2641 gacgcgccga ttgcaggcgc cctgcacgaa ctcgactacg agcgataccg gctgcgtcgc
     2701 gagtcgctcg aggccgtgct gttgcgcgcg ctcgcggagc cgcagtcgcg cgagaagtcg
     2761 gcgcaggtcc tgctgcgacc gccgctgccg ccgcgggtcg cgctcgacgg cgcgccggcg
     2821 gcatcccggc cggacggagt ccgccacccc gtcaccgtga cggtgttctg caatttcgag
     2881 tcgccacact gtgccgacgt gcagtccttg ctcgccgacc tgcgcgcgct gcaccccgag
     2941 gccatcgtcg tggcggcgcg cgacctgccg ctgccgatgc acgggctggc gccgctggcg
     3001 gcggaggcgg cgcggtgtgc ggggcgacag ggaaggtact ggccgttcca cgacctgctg
     3061 ttcgccggcc gtcgtgcgcc cgaccggacg gagctcgagc gggtcgccac ggcggttggc
     3121 gtggatcgcg agacgttcga tcgctgcctg gagacgcacg acaccgcgag cgaagtcggg
     3181 gcggacgtcg cgctcgcgca tcggctccgt ctcggcatgg tgcccgccgt cttcgtgaac
     3241 gggcgacgcg cggtggcccc ggtcacgatc gatcaactgc tgtggctggt gtccgccgag
     3301 ctggcgacgg ccgagccggc ccgcgccgtg gtcgctcccg cgagctcgct gccggtcagc
     3361 ctgcgcgcga cgctggtggg cgaggtgccg gggctcggcc tcgcgctcct cgaagtgaat
     3421 gggcggcgcc aggtcgtccg cgaaggcgag cgtgccacca gcaacgcgat cctgcggcgc
     3481 gtcgacgcgc gcggcgccga gctgctggtc gatggtcg
//
LOCUS       DASPFR010000058         6515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1361650, whole genome shotgun sequence.
ACCESSION   DASPFR010000058 DASPFR010000000
VERSION     DASPFR010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361650"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..819)
                     /locus_tag="VD737_01405"
     CDS             complement(37..819)
                     /locus_tag="VD737_01405"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HXW09246.1"
                     /translation="MPSPIETWLLALPWLLGAAALAWAWSVVIRNVTLVDTLWSLMFL
                     LAAVVYAYATSPAEARTVLLLALVALWSIRLAAYLGVRTLGHAEEDARYQAIRRRNEP
                     GFAWKSLYLVFGMQALLAWVISLPLLGAIAGEPRALGVLDAVGVALWLVGFTFETVGD
                     WQLARFKADPASAGRVMDGGLWRYTRHPNYFGDFCVWWGFYAIALAAGAWWSFVGPVI
                     MSVLLLRVSGVALLERHIGKRRPGYAEYARRTNAFFPGPRRG"
     gene            912..1424
                     /locus_tag="VD737_01410"
     CDS             912..1424
                     /locus_tag="VD737_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXW09247.1"
                     /translation="MPAALRRIRRPARTARGGVARVGLISDTHGRLRPEALEALRGCE
                     TVLHAGDVGRPEVLDGLRQLAPQVIAVRGNVDGEWAAGLPELAEVEIAGRRFLLLHDL
                     KQLGAAPRAGIDVVVSGHSHQPRIERRGDVLYVNPGSAGPRRFRLPVAVARLEISATR
                     LEAVIVELPV"
     gene            complement(1470..2450)
                     /locus_tag="VD737_01415"
     CDS             complement(1470..2450)
                     /locus_tag="VD737_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HXW09248.1"
                     /translation="MAKIPVFDDVKAAARRIAGHARKTPLLAGTPLDRLTGGCILLKL
                     ETLQHTGSFKFRGAWNRLAQLDERQRAAGVVAFSSGNHAQGVVEAARRLGIRATIVMP
                     SDAPRVKMQNTRDMGAEVVEYDRVHESREQIAAKIAGERGATLVPSFDDPDVIAGQGT
                     VGLEIAEQAGDLGLTLDDVIVCCSGGGLTAGIAVALEKLAPHAGLWTAEPEAHDDHRR
                     SLASGRRQRNDPGVPATICDALLAPMPGELTFAINESRLRGGLAVSDDEVRRAMAFAA
                     RTLKLVVEPGGAVALACVMSGKLDVRDRTVAITLSGGNVDDALLAEVLAG"
     gene            2500..3627
                     /locus_tag="VD737_01420"
     CDS             2500..3627
                     /locus_tag="VD737_01420"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09249.1"
                     /translation="MSVHESLPAGCEPACHGCRHRHLTPGESLAQKQRYLERTLAAWH
                     DRLEPVCSAGEASRYGYRDRVTLNARWEPAHGWRFGLMRRDELIAIPDCPVHTTRVNG
                     LLRLLRERLPPFAAVPLAYVHVAGAQATLIVKSRSVDPGLLDDLRAGLDASGMGGLWL
                     HCHPSAGRKLFARSGWQRLWGEAESSCHGGLVHGPTSFQQLLPGLHARALQTAARHLA
                     PRAVDAVLDLYCGIGASLRAWTDAGAATLGVELGGDAVDCARRNAPCAVVLRGTCVQR
                     LPQVREWWRRQVATRLVYVNPPRSGLEPELQSALAEELQPARVAYLSCSAGTLARDLA
                     SFEVAGYVVDRLVPYDFFPGTHHVECLALLSRCVSAPAPVG"
     gene            3690..5999
                     /locus_tag="VD737_01425"
     CDS             3690..5999
                     /locus_tag="VD737_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXW09250.1"
                     /translation="MCIPGRVAKVARDVLLAFLSITGVLVVAPAPAHAAATSAEDATR
                     AVVVPTRPGRPRIGLVLGGGGAKGAAHVGVLTMLEDMRVPVDCVIGTSMGALVGGTYA
                     AGMTAAEVDEAVAAISWAEAIGFAGWRQKLPMRRKLAGRTYSNSFEFGFRDGSVAAPS
                     GIINTQNIEQTIRHLVARSLGTSDFDQLPIPFRAIATDMLTGELVVLSGGDLAQAMRA
                     SMAVPGVFSPVTIDGRTLGDGSLTRNVAVDIARQTCADVVIAVAVPTPAPPPEALQSP
                     ITMIARTLDVLIGANERQQLESLGPQDVKIIVPMGDIGSGAFDRVAEAVPLGRRAAEE
                     QRESLRRYSLPAAEYQAWRESTRRPDSRQVRINDIQVVGVDRVAAAYVEANLGLEAGQ
                     QVDQKQLGEAINDVFALGDFDGVQYSLVGDAARPDLRVAVTEKALGPNILRFDLGLAV
                     GTAGANAFVLAADYLRPWINSRGGEVHGLLQVGRNALFHASLYQPVDVMHRWFVEPGA
                     RVSRSTEDLYIDDEIASRYNFDAVYAYLDAGHVFGTSAELRVGVRNGAQRATRDIAVP
                     GLPTLPTEAYGGLYASFTYDDRDREALATQGWLHRLRYFRGMDALGSRREYDRLEGVL
                     MRSWPLDDDLVTARAAGGASFQGELPFYDLFTLGGPISFPGLGLGQLRGTSYWTTQAS
                     YLHKVADLSPVFGQSLYAGVSLIAGDMSGRLDDVREPTLFGGSLLFGGYTAIGPLTLS
                     LSTTSSEDWSVLVRLGRPVEERTITDPTW"
     gene            complement(6156..>6515)
                     /locus_tag="VD737_01430"
     CDS             complement(6156..>6515)
                     /locus_tag="VD737_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09251.1"
                     /translation="DTLGKPVGQLAFDLPGCDDRMRLIAFRTVNALGEGDYYTGLAAT
                     MPGRACAAEDTLDAPLGSVADNLTREAMAWLASGACNVPMSADAATGARLKPAFEGAP
                     PRPFAPTPAERWLPGIQ"
BASE COUNT          915 a         2337 c         2338 g          925 t
ORIGIN      
        1 cctcttgact agccactagc cactgaccac gagccactat ccccgccgcg gtcccggaaa
       61 gaacgcattc gttcgcctgg cgtattccgc gtatccgggc ctgcgcttgc cgatgtgccg
      121 ctcgagcagc gcgacgccgg acacccgcag cagcagcacg gacatgatca cggggccgac
      181 gaacgaccac caggcgccgg cggcgagggc gatcgcgtag aagccccacc agacgcagaa
      241 gtcgccgaag tagttgggat ggcgcgtgta gcgccacagc ccgccgtcca tgacccggcc
      301 ggcgctcgcg gggtcggcct tgaaccgtgc gagctgccag tcgccgacgg tctcgaacgt
      361 gaacccgacc agccacaacg ctacgcccac cgcgtcgagc acgccgagcg cgcgcggctc
      421 accggcgatc gcgccgagca gcggcaacga gatcacccac gcgagcagcg cctgcatgcc
      481 gaacaccagg tagaggctct tccaggcgaa cccgggctcg ttgcggcggc ggatcgcctg
      541 gtaacgcgcg tcttcctcgg cgtggccgag cgtgcgcacg ccgaggtagg ccgcgagccg
      601 gatcgaccac agcgccacga gcgcgagcag cagcacggtg cgggcctcgg cgggcgacgt
      661 cgcgtacgca tagacgacgg ccgccagcag gaacatcaac gaccagagcg tgtcgaccag
      721 cgtgacgttg cggatcacca cgctccaggc ccaggcgagc gcggcggccc cgagcagcca
      781 gggcaaggcg agcagccagg tctcgatcgg tgacggcatg ggcgacgatc ccctcgggtc
      841 tgtggcgtgt tcgctcggct gcgcgcctcg caccttagtc gggggcgaga ggttcgggca
      901 agatcatagc catgcctgca gcactccggc gcatccggcg acctgcgcgc accgcgcgcg
      961 ggggcgtcgc tcgcgtgggc ctcatctcgg acacgcacgg caggctgcgc cccgaggcgc
     1021 tcgaggcgct gcggggctgc gagacggtgc tgcacgccgg tgacgtcggt cgtcccgagg
     1081 tgctcgacgg cctgcgccag ctggcgccgc aggtgatcgc cgtgcgcggc aacgtggacg
     1141 gtgagtgggc ggccggcctg ccggaactcg ccgaggtgga aatcgccgga cgccgcttcc
     1201 tgctgctgca cgacctgaag cagctcgggg ccgcgccacg cgcaggcatc gacgtcgtcg
     1261 tctccgggca ctcgcaccag ccgcggatcg agcgtcgtgg cgacgtgctc tacgtgaacc
     1321 cgggcagtgc cggcccgagg cgattccggc tgcctgtggc ggtcgcgcgg ctcgagatct
     1381 cggcgacgcg actcgaggcc gtcatcgtcg agctgccggt ctgagacacg ccgcccgcgc
     1441 gaacccgggc gagcgccggc ggcaaggatt cagccggcca ggacttcagc cagtagcgcg
     1501 tcgtcgacgt tgccacccga gagcgtgatg gcgaccgtgc ggtcgcgcac gtccagcttg
     1561 ccggacatca cgcacgccag cgcgaccgcg ccgcccggct cgacgaccag cttgagcgtg
     1621 cgggccgcga acgccatcgc gcgccggacc tcgtcgtccg acaccgcgag gccgccgcgc
     1681 aggcgcgatt cgttgatcgc aaaggtcagc tcgcccggca tcggcgccag cagcgcgtcg
     1741 cagatcgtcg ccgggacgcc gggatcgttg cgctggcgcc gcccggaggc gagcgagcgc
     1801 cggtgatcgt catgggcctc gggctccgcg gtccagagcc cggcgtgcgg cgcgagtttc
     1861 tcgagcgcaa cggcgatgcc tgcggtcagc ccgccgccgc tgcagcagac gatgacgtcg
     1921 tcgagcgtca gcccgagatc gccggcctgc tcggcgatct cgaggccgac cgtgccctgg
     1981 ccggcgatca cgtccgggtc gtcgaaagac ggcacgagcg tcgcgccgcg ctccccggcg
     2041 atcttcgcgg cgatctgctc gcgactctcg tgcacgcggt cgtactcgac cacctctgca
     2101 cccatgtcgc gcgtgttctg catcttcacg cgcggggcgt cgctcggcat cacgatcgtg
     2161 gcgcggatgc cgagccggcg agcggcctcg accacgccct gtgcatggtt gccggaggag
     2221 aacgcgacga cccctgcggc ccgctggcgc tcgtcgagct gggccagccg gttccacgcg
     2281 ccccggaact tgaacgaacc ggtgtgctgc agcgtctcga gcttgagcag gatgcaaccg
     2341 ccggtcaggc ggtcgagcgg cgtgcccgcg agcagcggcg tcttgcgcgc gtggccggcg
     2401 atccgccgcg cggcggcctt gacgtcgtcg aacaccggga tcttcgccat cgcctcatgc
     2461 ccctgtcggc atcgccggat tcatcgcacc atagcgcacg tgagcgtaca cgaatcgctt
     2521 ccggcgggct gcgagccggc gtgccacggc tgcaggcacc gtcacctgac acccggggaa
     2581 agcctcgcgc agaagcagcg ctacctcgaa cgcacgctcg cggcttggca cgaccgcctc
     2641 gaacccgtgt gctcggccgg cgaggcgtcg cgctacggtt accgcgaccg cgtcacgctc
     2701 aacgcccgct gggagccggc gcacggctgg cgcttcgggc tgatgcgtcg cgacgagctg
     2761 atcgcgatcc ccgactgccc ggtgcacacg acccgcgtca acggcctgtt gcgcctgttg
     2821 cgcgaacgac tgccgccgtt cgcggcggtg ccgctcgcgt acgtgcacgt ggcgggcgcg
     2881 caggccacgc tgatcgtgaa gtcgagatcg gtcgatcccg gactgctcga cgacctgcgc
     2941 gcaggcctcg atgcttccgg gatgggtggc ctgtggctgc actgtcatcc gtcggccggt
     3001 cgcaagctgt ttgcccgatc gggctggcag cgcctctggg gcgaggcgga gtcgtcgtgt
     3061 cacggtggac tggtgcacgg gccgacgtcg ttccagcagc tgctgccggg actgcacgcg
     3121 cgggcgctgc agaccgccgc gcggcacctg gcgccacgcg ccgtcgacgc cgtgctcgac
     3181 ctctactgcg gaatcggggc gagtctgcgg gcgtggacgg acgcaggcgc ggcgacgctc
     3241 ggtgtcgaac tcggcggcga cgcggtggac tgcgcgcgtc gaaacgcgcc gtgcgccgtc
     3301 gtgctgcgcg gcacctgcgt ccagcgcctg ccgcaggtgc gcgagtggtg gcggcgccag
     3361 gtggccacgc gcctggtcta cgtcaacccg ccgcgctcgg gcctcgagcc cgaactgcaa
     3421 tcggcgctcg ccgaggagct gcaacctgcc cgcgtcgcgt acctgtcatg cagcgccggc
     3481 acgctggcgc gcgatctcgc gtccttcgag gtcgccggct atgtcgtcga tcgactcgtc
     3541 ccctacgact tctttccggg cacgcatcac gtcgagtgtc tcgcgctgct gagccggtgc
     3601 gtgtccgcgc cggcgccggt cggatgacgc ggctgcggta ggatgccgcg cgtaccggcc
     3661 gagctcgcgg ccgggcagag gagctgccga tgtgcatccc gggccgcgtc gccaaggtgg
     3721 cgcgcgacgt cctgctcgca ttcctttcga tcaccggcgt gctcgtcgtc gcgcctgcgc
     3781 ctgctcacgc agccgccacc tcggccgagg acgcgacacg ggccgtggtg gtgccgacgc
     3841 gccctggacg cccacgcatc ggcctggtgc tcggcggcgg tggcgccaag ggcgcggcgc
     3901 acgtcggcgt gctgacgatg ctcgaggaca tgcgcgtgcc ggtcgactgc gtgatcggca
     3961 ccagcatggg cgcgctggtc ggcggcacct acgccgccgg catgacggcc gccgaggtcg
     4021 acgaggcggt ggccgccatc tcctgggccg aagccatcgg cttcgccggc tggcggcaga
     4081 agctgccgat gcgacgcaag ctggccggcc gcacctacag caacagcttc gagttcggct
     4141 tccgcgacgg cagcgtcgcc gcgccgagcg gcatcatcaa cacgcagaac atcgagcaga
     4201 cgatccggca cctcgtcgcg cgcagcctcg gcacgagcga cttcgaccag ttgccgatcc
     4261 cgtttcgcgc gatcgcgacc gacatgctga cgggcgagct ggtggtgctg tcgggcggcg
     4321 acctggcgca ggcgatgcgc gcgagcatgg cggtgcccgg cgtgttttcg cccgtgacga
     4381 tcgacggtcg cacgctcggc gacggcagcc tcacgcgcaa cgtcgccgtc gacatcgccc
     4441 gccagacctg cgcggacgtc gtgatcgcgg tcgccgtgcc cacgccggcg ccgccgcccg
     4501 aggcgctgca gtcgccgatc acgatgatcg cgcgcacgct cgacgtgctg atcggcgcca
     4561 acgagcgaca gcagctcgaa tcgctcgggc cgcaggacgt caagatcatc gtgccgatgg
     4621 gcgacatcgg ttcaggcgcg ttcgaccgcg tcgccgaggc cgtgccgctc ggtcggcgcg
     4681 cagccgagga gcagcgggag tcgctgcgcc gctattcgct cccggcggcc gagtaccagg
     4741 cgtggcgtga gtcgacccgg cgtcccgaca gccgccaggt gcggatcaac gacatccagg
     4801 tcgtcggggt ggaccgcgtc gctgcggcct acgtcgaggc caacctcggg ctcgaggccg
     4861 gacagcaggt ggaccagaag caactcggtg aagcgatcaa cgacgtgttc gcgctgggcg
     4921 atttcgacgg cgtgcagtac tcgctcgtcg gcgacgcggc acggcccgac ctgcgcgtgg
     4981 cggtcaccga gaaggccctc gggccgaaca tcctgcggtt cgacctcggc ctcgcggtcg
     5041 gtaccgccgg tgccaacgcg ttcgtgctcg cggccgacta tctccggccg tggatcaact
     5101 cgcgaggtgg cgaggtgcac ggactgctgc aggtgggacg caacgcgctc ttccacgcgt
     5161 cgctgtacca gccggtcgac gtgatgcacc gctggttcgt cgagccgggt gcccgggtct
     5221 cgcgctcgac ggaggatctc tacatcgacg acgagatcgc gagccgctac aacttcgacg
     5281 cggtgtacgc gtacctcgat gccggtcacg tgttcggcac gagcgccgaa ctgcgcgtgg
     5341 gggtgcgcaa cggcgcgcag cgcgccacgc gcgacatcgc ggtgccaggc ctgccgacgc
     5401 tcccgaccga agcctacggc gggctctacg cctcgttcac ctacgacgat cgcgatcgcg
     5461 aggcgctcgc gacgcaaggc tggctgcacc ggctgcgcta cttccgcggc atggacgcgc
     5521 tcggttcgcg tcgcgagtac gaccggctcg agggcgtgct gatgcggtcc tggcccctcg
     5581 acgacgacct ggtcaccgcg cgcgcggcgg gcggcgcgag cttccagggc gagttgccgt
     5641 tctacgacct gttcacgctg ggcggaccga tcagcttccc gggcctcggg ctcgggcagc
     5701 tgcgcggcac ctcgtactgg acgacgcagg cgagctacct gcacaaggtc gcggacctga
     5761 gcccggtgtt cggccagtcg ctctacgcgg gagtgagcct gatcgccggg gacatgtcgg
     5821 ggcgactgga cgacgtgcgc gagccgaccc tgttcggcgg ctcgctgctg ttcggcggct
     5881 acacggcgat cgggccgctc acgctgtcgc tgtcgacgac gagctcggag gactggagcg
     5941 tgctggtgcg gctcggccgc ccggtcgagg agcgcacgat caccgatccc acgtggtgag
     6001 cggcgccgtc accaccgcgg cgggcgaagg ccggctgatg gcaggaatgc cgggtgcggc
     6061 gtccgacaaa tcgcgccaga tatagaccgg acatcccgaa cgccgctccc ttgcgtcggt
     6121 gccactctcg ccgacgtacc gacgcgcgtg gtgactcact ggataccagg cagccagcgc
     6181 tccgcgggtg tcggcgcgaa cggcctcggc ggcgcgcctt cgaacgccgg cttcagtcgc
     6241 gcgcccgtcg cggcgtcggc gctcatcggc acgttgcagg cgccgctcgc gagccacgcc
     6301 attgcctcgc gcgtcaggtt gtcggcgacc gagcccagcg gcgcgtcgag cgtgtcctcg
     6361 gcggcgcacg cgcgacccgg catcgtcgcg gcaagacccg tgtagtagtc gccctcgccg
     6421 agggcgttga cggtccggaa cgcgatcagc cgcatccggt cgtcgcaacc cggcaggtcg
     6481 aaggcgagtt gccccacggg cttgccgagc gtgtc
//
LOCUS       DASPFR010000059         7841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_141703, whole genome shotgun sequence.
ACCESSION   DASPFR010000059 DASPFR010000000
VERSION     DASPFR010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141703"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1426)
                     /locus_tag="VD737_01435"
     CDS             complement(149..1426)
                     /locus_tag="VD737_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HXW09252.1"
                     /translation="MGDVPVSGLAILLLLLLCLSAFFSGTETALMSVDRYRLRHLARE
                     GSQAARMMERLLERPDRLIGTILVCNNFVNTAAAAIVTLIALQLGGEAYAAIGVGVFT
                     VLLIIFGEVAPKTFGALYPERIALPAAVVYTGLLKALYPIVLATNFVANGVLRAFGVT
                     RERADRMSLSREELRTVVAEASTVIPHRHQRMLMSILDLEKMNVEDIMVPRHEIFGID
                     VNDDWDDIVEQIRDSRHTRIPVYDGNLESLLGILHMKKVARLLALDEFDRDKLIGIAK
                     AREPYYVPEGTSLNTQLLQFQRQRRRVAFVVDEYGDVQGLITLEDLLEEIVGEFTSDT
                     SLLHKDVHRERTDSFVVNASASVRTLNRKMGWALPTAGARTLNGLIVEQMETIPSPGQ
                     RLRIGDYSLEVLQVTDNAVKTVRLKPVAVSPAE"
     gene            complement(1439..2260)
                     /gene="ccsA"
                     /locus_tag="VD737_01440"
     CDS             complement(1439..2260)
                     /gene="ccsA"
                     /locus_tag="VD737_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HXW09253.1"
                     /translation="MLLPLIAVACYLLGAAGLAVATYQNRGEPHSAGGRGRRIASVGI
                     ASVGVLVHVAALLDERRVAPSAALSLGDTLALVSLVVAITSILMALRPGKRGMAALLL
                     FIAAVLEAAFSEGSRQFSVGRPGWELAFHVAMATIAFAFLTIGMALAIAQVIVARRVR
                     SRQPLGWLKILTPIESLESGTFQSILAGFAVLTLALVSGAFFIENLFAQHLVHKVVLA
                     IVAWLVFGVLLLGRLRFGWRGRKALRWTLAGYTLLGLSYFGSKIILENVLGKHWG"
     gene            2416..3768
                     /gene="ffh"
                     /locus_tag="VD737_01445"
     CDS             2416..3768
                     /gene="ffh"
                     /locus_tag="VD737_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HXW09254.1"
                     /translation="MFDKLSARLASVVEGLRGRGRLTEENIGDTLRQVRMALLEADVA
                     LPVVKTFIDGVKARVVGQEVHKSLTPGQALIKIIHEELVRVMGEGVRPLNLRVQPPAI
                     VLLAGLQGAGKTTTAAKLALWLKTRERKRVLLVSTDVRRPAALLQLERLGQQLDIDVA
                     PASATQAPLAIAQQALAQARRGVYDVLLVDTAGRLHVDEQMMAEVRELGAALDPVETL
                     FVVDSMAGQDAVNAARAFGVALNLTGVVLTKADGDARGGAALSVRQITGQPILFVGTG
                     EKPEALEAFQPERMASRILGMGDVLSLVEEVTRQVDRAEAEKLARKVAKGKDFNLEDL
                     LSQLRQLEQMGGVSALMDKLPAQLTAKAAQLPQNNEKEVKRQIAIICSMTPQERRYPK
                     NIDGSRKRRIAAGSGTHVAEINRLLKNFLQMQKMMKGMGKGGLGRMMRAMGGRMPFSG
                     "
     gene            3961..4227
                     /gene="rpsP"
                     /locus_tag="VD737_01450"
     CDS             3961..4227
                     /gene="rpsP"
                     /locus_tag="VD737_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005408594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HXW09255.1"
                     /translation="MVTIRLQRRGGKKEPFYHVVVTDSRRAGGGSLEQLGFYNPVARG
                     NSEELRLDLARVDYWIGVGAQVSDRVAQLVKTYRKSQPTAAAAA"
     gene            4263..4751
                     /gene="rimM"
                     /locus_tag="VD737_01455"
     CDS             4263..4751
                     /gene="rimM"
                     /locus_tag="VD737_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HXW09256.1"
                     /translation="MVLGRIGAPFGVQGWVKVTSYTEPAAGIAGYAQWTLVRDGETRS
                     VRVLESKRAGHSVAVRLEGVETREAAQALTGFEVQVDRSELPPTAPNEHYLHDLLGLE
                     AVNRDGVPLGRVDGFLELPAHPVAVLRGDERERLVPVVRDRLVAVDLAAGRVTFDWHP
                     DD"
     gene            4773..5534
                     /gene="trmD"
                     /locus_tag="VD737_01460"
     CDS             4773..5534
                     /gene="trmD"
                     /locus_tag="VD737_01460"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009683443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HXW09257.1"
                     /translation="MRIVVVTLFPEFVEHALGFGVLGRAIERGVLAVETVNPRQFATD
                     VHKTVDDRPYGGGPGMVLKAEPLRQATELASARVPAARRVYLAADGVALTQAKVAEYA
                     ALPALVLVAGRYEGVDERFVEECIDESVSVGDVVLSGGELPALTVIDAVARLLPGTLG
                     DATSALQESFSDGLLDWPHYTRPEVWAGRVVPEVLAGGNHAAIARWRLKQALGRTWQR
                     RPDLLARRNLTDVERRLLAEYQAEQDATRREQGQN"
     gene            5550..5894
                     /gene="rplS"
                     /locus_tag="VD737_01465"
     CDS             5550..5894
                     /gene="rplS"
                     /locus_tag="VD737_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010220244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HXW09258.1"
                     /translation="MSNIIATLNAERMTRKVPDFGPGDTVVVEVKVKEGDRERVQAYE
                     GVVIAKSNRGINSSFTVRKISHGEGVERVFQTYSPALGNITVKRRGNVRRAKLYFLRE
                     RTGKSARIEEKV"
     gene            5914..7761
                     /gene="sppA"
                     /locus_tag="VD737_01470"
     CDS             5914..7761
                     /gene="sppA"
                     /locus_tag="VD737_01470"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HXW09259.1"
                     /translation="MRAIRKLLHWLWVGLDGLRRVLHLLLLLLIFGLIGALFSRPIPF
                     VPDKAALVIAPQGPLVEQFTGDPVERAVADALRQGPVETRLRDLVEAIEAAKDDRRIV
                     ALYLDLGGLAGGGTAKLQEVAKAIDEFRTSGKPVIAFGEYYDQPQYYLAARADEIYLD
                     PQGIAYIDGFANYGMFVKDALDKLSIDWNVFRVGQYKSAVEMFTRNDMSPAEREESLA
                     WLNSVWSTWKADVAKARGLEPAALQAYADNAPAALRAAGGDLAKVAFDAGLVTGLKGR
                     YEVEDRIAEITGEDEDQHSFHGVAYEGYLANVHSSKALESESGNKIAYIVASGEILPG
                     EQAPGTIGSDTLAALLREARYDDDVKAVVLRIDSPGGSTFASEVIRREVAELRADGKP
                     VVASMSTLAASGGYYIAMDADRIVASPATLTGSIGIFAMFPTFQRSLERLGVHVDGVG
                     TTALSGEFSPVRPMSDATRDVLQQSIDHEYERFITLVAKSRKKEVAAVDRIAQGRVWS
                     GADAKRLGLVDQLGGPEEAIELAAKLADLGKDYHVEHYDRPVGIGEALGFRIDAAISR
                     LVAPLVARHVAIPQLPRALEPLAQELARLDRLRDPRNVYMYCVACRAER"
BASE COUNT         1285 a         2612 c         2794 g         1150 t
ORIGIN      
        1 ccgtccgcga catggatgtc gcggccggag cccccaagga tgggttcacg gcgtcccgcc
       61 gcggaagatg tcgtggaggg cccgacggcc gcagcacaag cgcttccagc gatcatgccc
      121 gaatccgcgc tgcccgcgac ctccggcgct actctgctgg tgataccgcg accggcttca
      181 ggcgtacggt cttcaccgcg ttgtcggtga cctgcagcac ctcgagcgag tagtcgccga
      241 tccgcaggcg ctgtcccggc gacggaatcg tctccatctg ctcgacgatg aggccgttca
      301 gcgtgcgcgc gcccgcggtg ggcaacgccc agcccatctt gcggttgagc gtacgcacgc
      361 tcgccgaggc attgacgacg aagctgtcgg tgcgttcgcg atgcacgtcc ttgtgcagga
      421 ggctcgtgtc cgaggtgaat tcgccgacga tctcctcgag caggtcctcg agcgtgatca
      481 gcccctgcac gtctccgtac tcgtcgacga cgaaggcgac gcggcgccgc tgccgctgga
      541 actgcagcag ttgcgtgttg agcgaggtgc cctcgggcac gtagtacggc tcgcgcgcct
      601 tcgcgatgcc gatcagcttg tcgcggtcga attcgtcgag cgcgagcagc cgcgcgactt
      661 tcttcatgtg caggatgccg agcagcgatt ccaggttgcc gtcgtagacc gggatgcgcg
      721 tgtggcggct gtcgcggatc tgctcgacga tgtcgtccca gtcgtcgttg acgtcgatgc
      781 cgaagatctc gtggcgcggc accatgatgt cctcgacgtt catcttctcg aggtcgagga
      841 tgctcatcag catgcgctgg tgacggtgcg ggatcaccgt gctcgcctcg gccaccacgg
      901 tccgcagctc ctcgcggctc aggctcatgc ggtcggcgcg ctcgcgagtg acgccgaagg
      961 cccgcagcac gccgttggcc acgaagttgg tggccagcac gatcgggtac agcgccttca
     1021 gcagcccggt gtagacgacg gcggccggca gcgcgatgcg ctcggggtag agcgcgccga
     1081 aggtcttggg cgccacctcg ccgaagatga tcaggagcac ggtgaagacg cccacgccga
     1141 tcgccgcgta cgcctccccg ccgagctgca gtgcgatcag cgtgacgatc gccgccgcgg
     1201 ccgtgttcac gaagttgttg cagaccagga tcgtgccgat cagccggtcc ggtcgctcga
     1261 gcagtctttc catcatgcgc gcggcctggc tgccttcacg cgccaggtgg cgcagccggt
     1321 accggtccac gctcatcaat gccgtttcgg tgccggaaaa gaatgccgac aggcacagca
     1381 gcaacagcag gaggatggcg agaccgctga cgggtacgtc gcccatggcg gctgtccgtc
     1441 agccccagtg cttgccgagc acgttctcga ggatgatctt gctgccgaag tacgacagcc
     1501 caagcagcgt gtagcccgcc agcgtccacc gcagcgcctt gcggccgcgc cacccgaacc
     1561 tgagccggcc gagcagcagc acgccgaaca cgagccacgc gacgatcgcg agcacgacct
     1621 tgtgcacgag gtgctgcgcg aacaggttct cgatgaagaa cgcgccgctg acgagcgcga
     1681 gcgtcagcac ggcgaagccg gccaggatgc tctggaacgt cccggattcg agcgactcga
     1741 tcggcgtcag gatcttgagc cacccgagcg gctgccggct gcgcacgcgg cgcgccacga
     1801 tcacctgcgc gatggcgagc gccatgccga tggtcaggaa cgcaaatgcg atggtcgcca
     1861 tcgcgacgtg gaacgcgagt tcccagcccg gacgaccgac cgagaactgg cggctgccct
     1921 cggagaacgc cgcttcgagc acggcggcga tgaacagcag cagcgccgcc atgccccgct
     1981 tgccgggccg caacgccatg aggatcgatg tgatcgcgac gacgaggctg acgagggcca
     2041 gcgtgtcgcc gagcgacagc gccgccgagg gtgccacccg tcgctcgtcg agcagcgcgg
     2101 cgacgtgcac gaggacgccg acgctcgcga tgccgacgga cgcgatgcgc cttccacggc
     2161 cccccgccga gtgtggctcg ccgcggttct ggtacgtggc gacggcgagc ccggcggccc
     2221 cgagcaggta acaggcgacg gcgatcagtg gcagcagcaa gcgcggaacc ggcggttgag
     2281 agtgaccacc gcatgatgcc agagagccgc cgggctgagg agaacggcaa accgaccctc
     2341 ccggccctct ataatcgccg tttcccgcgc ccggccgagc ccggacgcac tccagcccgc
     2401 gaggtggtcc cgcccgtgtt cgacaagctc tctgcgcgcc tcgcgtccgt cgtcgaaggc
     2461 ctgcgcggcc gcggccgcct cactgaggaa aacatcggtg acacgctgcg gcaagtgcgc
     2521 atggcgctgc tcgaggcgga cgtggcgctg cccgtcgtca agaccttcat cgacggcgtc
     2581 aaggcgcgcg tggtcggcca ggaagtccac aagagcctga cgcccggcca ggcgctgatc
     2641 aagatcatcc acgaggaact cgtccgcgtg atgggcgagg gcgtgcgccc gctgaacctg
     2701 cgcgtgcagc cgcctgccat cgtgctgctc gcgggcctgc agggcgccgg caagaccact
     2761 acggccgcca agctcgcgct gtggctcaag acgcgcgaaa ggaagcgcgt gctgctcgtg
     2821 tcgaccgacg tgcgtcgccc ggcggccttg ctgcagctcg agcgcctcgg ccagcagctc
     2881 gacatcgacg tcgcgcccgc gagcgcaacg caggcgccgc tcgcgatcgc gcagcaggcg
     2941 ctcgcgcagg cgcgccgcgg cgtctacgac gtgctgctcg tcgacacggc ggggcggctg
     3001 cacgtcgacg agcagatgat ggccgaggtg cgcgaactcg gcgccgcgct cgacccggtc
     3061 gagacgttgt tcgtcgtcga cagcatggcg ggccaggacg cggtgaacgc cgcgcgcgcg
     3121 ttcggcgtcg cgctgaacct caccggagtc gtgctcacga aggccgatgg cgacgcccgc
     3181 ggcggcgccg cgctgtcggt gcggcagatc accgggcagc cgatcctgtt cgtcggcacg
     3241 ggcgaaaagc cggaggcgct ggaggcgttc cagcccgagc gcatggcttc gcgcatcctc
     3301 ggcatgggcg acgtgctgtc gctggtggag gaggtcacgc gccaggtcga ccgcgccgag
     3361 gccgagaagc tcgccaggaa ggtcgccaag ggcaaggatt tcaacctcga ggacctgctc
     3421 tcccagctgc gccagctcga gcagatgggg ggtgtgtccg cgctgatgga caagcttccc
     3481 gcccagctga cggccaaggc ggcgcagctg ccgcagaaca acgagaagga ggtcaagcgg
     3541 cagatcgcca tcatctgctc gatgaccccg caggagcggc gttacccgaa gaacatcgac
     3601 ggttcgcgca agcgccggat cgcggcgggc tccggtacgc acgtggccga aatcaaccgg
     3661 ctgctcaaga acttcctgca gatgcagaag atgatgaagg gcatgggcaa gggcgggctc
     3721 gggcgcatga tgcgggccat gggggggcga atgcccttca gcggttagcg gtcagtaggg
     3781 gcgggtccgg ggaaggcgca cagccgtgac ctctgactaa ccgctatccg cttcttcaag
     3841 taaccgccag ccacttcctc aagtatttct ttcgtcacga tttttcgtta tagttcgcgg
     3901 ctcttttttg gccgggcagc ctggatgccg cccggccgcg aacctgaggc ttgcatcaag
     3961 atggtgacga tccgtttgca gcgccgcggc ggcaagaagg aacccttcta ccacgtggtc
     4021 gtgaccgaca gccggcgcgc cggcggtggc tcgctcgagc agctgggctt ctacaacccg
     4081 gtcgcgcgcg gcaacagcga agagctgcgc ctcgacctcg cccgcgtgga ttactggatc
     4141 ggcgtgggcg cgcaggtttc cgaccgggtc gcgcagctgg tcaagaccta ccgcaagtcg
     4201 cagccgacgg ccgctgcggc ggcctgagcc tcgtgtcggc gcgcaccccg ggcgctcgcg
     4261 ccgtggtgct gggccgaatc ggtgccccgt tcggggtgca gggctgggtc aaggtgacgt
     4321 cgtacaccga gcccgcggcc gggatcgccg ggtacgcaca gtggacgttg gtacgggacg
     4381 gcgagacgcg cagcgtccgg gtgctggagt cgaagcgggc cggacattcg gtggcagtca
     4441 ggctggaggg cgtggaaaca cgcgaggcgg cgcaggcgct gaccgggttc gaggtgcagg
     4501 tcgaccgttc cgagttgccg ccgacggcgc cgaacgaaca ctacctgcac gacctgctgg
     4561 ggctcgaagc ggtcaaccgc gacggagtgc cgctcgggcg cgtggatgga tttctcgagt
     4621 tgccggcgca ccccgtcgca gtgctgcggg gggacgaacg ggaacgactg gtgccggtgg
     4681 tccgcgaccg actcgtcgcc gtggacctcg cggcggggcg ggtgaccttc gactggcacc
     4741 cggacgattg acggccgggg acaggtaaca cgatgcgcat cgtcgtcgtg acgctgttcc
     4801 cggagttcgt cgagcacgcc ctcggattcg gggtgctcgg acgagcgatc gagcgtggcg
     4861 tgctggcggt ggaaaccgtg aatccgcgcc agttcgcgac ggacgtgcac aagacggtcg
     4921 acgaccggcc ttacggcggc ggccccggca tggtgctgaa ggccgagccg ctgcggcagg
     4981 ccacggagct cgcgtccgcg agggtgcccg cggcgcgccg ggtgtacctg gccgcggacg
     5041 gcgtcgcgct cacgcaggcc aaggtcgcgg agtacgcggc cctgccggcg ctggtactcg
     5101 tggcgggtcg ctacgagggg gtggacgagc ggttcgtcga ggaatgcatc gacgagtcgg
     5161 tctcggtcgg tgacgtggta ctgagcggcg gcgagctgcc ggcgctcacg gtgatcgacg
     5221 cggtcgcgcg actgttgccg ggcacgctcg gtgacgccac gtcggcgctg caggagtcgt
     5281 tcagcgacgg cctgctcgac tggccgcact acacccgccc cgaggtgtgg gcggggcggg
     5341 tggtgccgga ggtgctggcg ggcggcaacc acgcggccat cgcgcgctgg cgactgaagc
     5401 aggcgctcgg tcgcacgtgg cagcggcgac ccgacctgct ggcgcggcgg aatctgacgg
     5461 acgtggagcg caggctcctc gcggaatacc aggcggagca ggacgcgacg cgacgtgagc
     5521 agggacagaa ctgaagacaa ggtgaagaaa tgagcaacat cattgccacg ctcaatgccg
     5581 agcgcatgac ccgcaaagtg ccggacttcg gcccgggcga cacggtcgtc gtcgaggtga
     5641 aggtcaagga aggcgaccgt gagcgcgtcc aggcctacga aggcgtcgtg atcgcgaaga
     5701 gcaaccgcgg catcaactcc tcgttcaccg tgcgcaagat ctcgcacggc gagggcgtgg
     5761 agcgcgtgtt ccagacgtac agcccggcgc tcggcaacat caccgtgaag cgtcgcggca
     5821 acgtgcgtcg cgccaagctg tacttcctgc gcgagcgcac gggcaagtcc gcccgcatcg
     5881 aagagaaggt ctgacgaggg cgggcgcgcc ggcatgcgtg ccatccgcaa gctgctgcac
     5941 tggctctggg tgggcctcga cggcctgcgc agggtcctgc acctgctgct gctgctgctg
     6001 atcttcggcc tgatcggtgc gctcttctcc cgtcccatcc cgttcgtgcc cgacaaggcg
     6061 gcgctggtca tcgcgccgca gggaccgctc gtcgagcagt tcaccggcga cccggtcgag
     6121 cgtgctgtcg cggacgctct gcgccagggg ccggtcgaga cgcgcctgcg cgacctcgtc
     6181 gaggcgatcg aagcggcgaa agacgacagg cgcatcgtgg cgctgtacct cgacctgggc
     6241 ggcctggccg gcggcggcac cgccaagctg caggaagtcg cgaaggcgat cgacgagttc
     6301 cgcaccagcg gcaagccggt gatcgcgttc ggcgagtact acgaccagcc gcagtactac
     6361 ctggcggcgc gcgccgacga gatctacctc gatccccagg gcatcgccta catcgacggc
     6421 ttcgcgaact acgggatgtt cgtgaaggac gcgctcgaca agctgtcgat cgactggaac
     6481 gtcttccggg tcggccagta caagtcggcg gtcgagatgt tcacccgcaa cgacatgtcg
     6541 cccgccgagc gcgaggaaag cctggcgtgg ctcaactcgg tctggagcac gtggaaggcc
     6601 gacgtcgcca aggcgcgcgg gctcgaaccg gccgcgctcc aggcctatgc ggacaacgcc
     6661 ccggctgccc tgcgcgcggc gggaggggac ctcgcgaagg ttgcgttcga cgcgggactc
     6721 gtcaccgggc tcaagggccg ctacgaggtc gaggatcgca tcgcggagat caccggcgag
     6781 gacgaggacc agcactcctt ccacggcgtg gcttacgagg gctacctcgc gaacgtccac
     6841 tcgtcgaagg cgctcgagag cgagtccggc aacaagatcg cgtacatcgt cgcctccggc
     6901 gagatcctgc cgggcgagca ggcgcccgga acgatcggct cggacacgct ggcggcactg
     6961 ctgcgcgagg cgcgctacga cgacgacgtg aaggcggtgg tattgcgcat cgacagtccc
     7021 ggcggcagca cgttcgcctc ggaagtgatc cggcgcgaag tcgccgaact gcgcgccgac
     7081 ggcaagccgg tcgtcgcctc gatgagcacg ctcgcggcct cgggcggcta ctacatcgcg
     7141 atggacgcgg accgcatcgt cgcgagcccg gcgaccctca ccggctcgat cggcatcttc
     7201 gcgatgttcc cgaccttcca gcgctcgctc gagcgcctcg gcgtgcacgt cgacggggtc
     7261 ggcacgacgg ccctgtcggg cgagttcagc ccggtgcggc cgatgagcga cgcgacccgc
     7321 gacgtgctgc agcagagcat cgaccacgag tacgaacgct tcatcacgct ggtcgcgaag
     7381 tcgcgcaaga aagaggtggc cgcggtcgac cgtatcgccc agggccgcgt ctggtcgggc
     7441 gccgacgcga agcggctcgg gctcgtcgac cagctcggcg gccccgagga ggcgattgaa
     7501 cttgccgcca agctcgccga cctgggcaag gactaccacg tcgagcacta cgaccggccg
     7561 gtcggtatcg gcgaggcact cggcttccgc atcgacgccg cgatctcgcg cctggtcgcg
     7621 ccgctggtgg cccggcacgt cgccatcccg cagctgccgc gcgcgctcga gccgctggcc
     7681 caggagctcg cgaggctcga ccggctgcgc gacccgcgca acgtctatat gtactgcgtg
     7741 gcgtgcaggg cagagcgatg aaggaagttt ctggtgtcta gtgtctagcg gttagtcgga
     7801 gcgaccacct cgacgtcgcg atgcctacta accgctagac a
//
LOCUS       DASPFR010000060         4684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_141795, whole genome shotgun sequence.
ACCESSION   DASPFR010000060 DASPFR010000000
VERSION     DASPFR010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141795"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VD737_01475"
     CDS             <1..663
                     /locus_tag="VD737_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXW09260.1"
                     /translation="LPARGFHAALRTLTRERGTLLLIDETHTISTGPGGYTRAHGLEP
                     DLFVLGKPIASGVPASVWGFTDEVARRLDAVRAATPAGHSGMGTTLSANALSLAAMRA
                     TLEHVMTPAAYGAMERLASVLELNLEQAIEEYAVPWHVVRVGARVEFVCAPGPLRNGA
                     EAEVAHAPALERAIHLSLLNDGCLVAPFHNMMLVCPSTTEDQVGRLCEAFERVLARLT
                     AA"
     gene            701..2089
                     /locus_tag="VD737_01480"
     CDS             701..2089
                     /locus_tag="VD737_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXW09261.1"
                     /translation="MAADIPLLETLRAIVGNDHVLTGDQVGARAVGWADHSPCRALAI
                     VRPGRTEEVSRVLAACHAAGQPVVTFGGLTGLVRGAVAGPEEIALSLERMHTIEAVDV
                     VGRTMTVEAGVPLQRVQEAAEQHGLLYPVDFGARGSAQIGGSVATNAGGNGVIRYGMT
                     RESVLGLEAVLADGTVVSAMNRMLKNNAAYDLKQLFIGTEGTLGIVTRLVLRLRELPR
                     STQTALVACDRFGAVTQLLKHVDAALGGGLSAFELMWNEFYRLVTTPPAKGSPPLSQD
                     HPYFVLVESSGGDAARDRAQFEDAMAAAIEQGLVVDAVIAESEAQREALWALRDDVES
                     FFRMGMPVVFDVSLPIAEMEGYVAEVVARLEDEWPAYRRFVFGHLGDGNLHVIATGPP
                     SLEARHGIERCVYEPLAARHGSVSAEHGIGLEKQPWLAQCRTPAELALMRRLKDALDP
                     KAILNRGRVLTV"
     gene            complement(2147..2722)
                     /locus_tag="VD737_01485"
     CDS             complement(2147..2722)
                     /locus_tag="VD737_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HXW09262.1"
                     /translation="MLQRKFRPIVAAAATVVIALGASGVASAQVVPGKPGTMNIVEVA
                     MAVNQANGEFDYLLAAATCPYFEGAVAALLSGNDRYTLFAPTDAAFEALQRELGVETP
                     APDVTCTLPQATVFNILAYHVTEGRRFSNSVFNKRSVKMIEMLNDQYIVTNPNLTIED
                     NAGQTIRPVLPLVNIKASNGVIHVIDTVLMP"
     gene            complement(2897..3772)
                     /locus_tag="VD737_01490"
     CDS             complement(2897..3772)
                     /locus_tag="VD737_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXW09263.1"
                     /translation="MHPDVKGFHHAGTGSWTYVIADPRSGAAAIVDPVLDFDWKSVRT
                     ATTAADEVVEHCQRQGLDVRWILETHAHADHLSAAQHLKGRLAGEVAIGRGILDVQRT
                     FKRIYGLGHDFEPDGRQFDRLLDDGESLPLGSMTIRVLATPGHTSDSVSFVAGDAAFI
                     GDTLFMPDSGSARCDFPGGDARQLYHSIVHRLYALPPATRVYVCHDYRPGGREARCET
                     SVAEQRRTNVHLRDGVDEAAFVRLRTERDATLEVPNLIVAAVQVNIRAGHLPPPEQDG
                     VRYLRVPLDVFGRAR"
     gene            complement(3799..4602)
                     /locus_tag="VD737_01495"
     CDS             complement(3799..4602)
                     /locus_tag="VD737_01495"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXW09264.1"
                     /translation="MNGSPAPAGGVTRSAGEGSITVVGSYVSPFVRKVLACLELKQLD
                     YRIDPIAPFYGDDEYSRLSPLRRVPILIDDRVTLCDSTVIVQYLEDRYPERHALYPAD
                     VADRARARWLEEYADTRLADVVVWGVFNKAVLDPGIWGRPRDLAAIERVMQDELPGVL
                     DYLESQVPDTGFLFDPATIGIADIALAAPFRNLGFARQRVDAERWPRTAAFVDRVLAH
                     EAFAKFRPFEERQLRTPIAQQRAVLAAMGAPLTGRSLGTDAPRRGPMTR"
BASE COUNT          682 a         1586 c         1686 g          730 t
ORIGIN      
        1 ctgccggcgc gcggcttcca cgcggcgctg cgcacgctga cgcgcgagcg cggcacgctg
       61 ctgctgatcg acgagaccca cacgatctcg acgggcccag gcggctacac gcgcgcccac
      121 ggcctggaac cggacctgtt cgtgctcggc aagccgatcg ccagcggcgt gcccgccagc
      181 gtctggggtt tcacggacga ggtcgctcga cggctcgacg cggtgcgcgc cgcgacgccg
      241 gccggtcact ccggcatggg caccacgctg tctgcgaacg cgctttcgct cgctgcgatg
      301 cgcgccacgc tcgagcacgt catgacgccg gcggcctacg gcgccatgga gcgcctcgcg
      361 agcgtgctcg agctcaacct cgagcaggcg atcgaggaat acgccgtgcc gtggcacgtc
      421 gtgcgcgtcg gggcccgcgt cgagttcgtc tgtgcgcccg gcccgctgcg caacggcgcc
      481 gaagccgagg tcgcgcacgc accggcgctc gagcgcgcga tccacctgtc gctgctgaac
      541 gacggctgcc tggtcgcgcc gttccacaac atgatgctcg tctgcccgtc gacgacggag
      601 gaccaggtgg gtcgtttgtg cgaggcgttc gagcgcgtgc tcgcgcgact gaccgccgcc
      661 tgagtcaccg tcgtcgccct ggcaaccgat cggaggacgc atggccgctg acataccgct
      721 gctcgaaacg ctgcgcgcga tcgtgggcaa cgatcacgtg ctcacgggcg accaggtcgg
      781 cgcccgcgcc gtcggctggg cggaccacag cccgtgccgc gccctggcga tcgtccgccc
      841 gggccgcacg gaggaagtct cgcgcgttct cgcggcgtgc cacgcggccg gccagcccgt
      901 ggtcaccttc ggcggcctca ccggcctggt ccgcggcgcg gtggccggcc ccgaggagat
      961 cgcgctgtcg ctcgagcgca tgcacacgat cgaagccgtc gacgtcgtcg ggcgcacgat
     1021 gacagtggag gcaggcgtgc cgctgcagcg ggtacaggag gcggccgagc agcacgggct
     1081 gctctacccg gtcgacttcg gcgcgcgcgg ctcggcacag atcggcggca gtgtcgcgac
     1141 caacgccggc ggcaatggcg tcatccgcta cggcatgacg cgcgaatccg tgctcggcct
     1201 cgaggccgtg ctcgccgacg gcaccgtcgt ctcggcgatg aaccgcatgt tgaagaacaa
     1261 cgcggcgtac gacctgaagc agctgttcat cgggaccgag ggcacgctcg gcatcgtgac
     1321 gaggctggtg ctgcgattgc gcgaactgcc gcgcagcacg cagaccgcgc tggtggcgtg
     1381 cgatcggttc ggcgcggtca cgcagctgct caagcacgtg gatgccgcgc tcggcggcgg
     1441 cctgagcgcg ttcgaactga tgtggaacga gttttatcgc ctggtgacga cgccgccggc
     1501 caagggttca ccgccgctgt cgcaggacca tccctacttc gtgctggtcg aatcctccgg
     1561 cggcgatgcc gcccgcgacc gcgcgcagtt cgaggacgca atggccgccg cgatcgagca
     1621 ggggctcgtc gtcgacgcgg tgatcgccga atcggaggcg cagcgcgaag cgctctgggc
     1681 actgcgcgac gacgtcgaga gcttcttcag gatgggcatg ccggtcgtgt tcgacgtgag
     1741 cctgccgatt gccgagatgg aaggttacgt cgcagaggtc gttgcgcgac tcgaagacga
     1801 gtggccggcg taccgtcgat tcgtgttcgg ccacctgggc gacggcaacc tgcacgtgat
     1861 cgcgaccggc ccgccgagcc tcgaggcccg ccacggcatc gagcgttgcg tctacgagcc
     1921 cctcgccgca cggcacggct cggtctccgc cgaacatggc atcggcctgg agaagcagcc
     1981 gtggctcgcc cagtgccgca cgccggccga acttgcgctg atgcgcaggc tcaaggacgc
     2041 gctggacccc aaggccatcc tcaatcgcgg tcgcgtgctg accgtctgag accgacgcgc
     2101 catgacgcga aggccgcgcg cgtcgcgcgc gcgcggcccc cgcaggtcag ggcatcagca
     2161 ccgtgtcgat cacgtggatc acgccgttgc tcgccttgat gttgacgagc ggcagcacgg
     2221 gtcgaatcgt ctggcccgcg ttgtcctcga tcgtgaggtt cgggttcgtc acgatgtact
     2281 ggtcgttcag catctcgatc atcttgaccg agcgcttgtt gaagacgctg ttcgagaaac
     2341 gccggccctc ggtgacgtgg tacgcgagga tgttgaacac cgtcgcctgc ggcagcgtgc
     2401 aggtgacgtc cggtgccggc gtttcgacgc cgagctcgcg ctgcagggcc tcgaaggccg
     2461 cgtcggtcgg cgcaaacagc gtgtagcgat cgttgccgga aagcagggcg gcgaccgcgc
     2521 cctcgaagta cggacaggtg gccgccgcca gcaggtagtc gaattcgccg ttggcctggt
     2581 tcacggccat ggcgacttcg acgatgttca tcgtgccggg cttgccgggc acgacctggg
     2641 cgctggcgac gccggacgcg ccgagcgcga tcacgacggt ggccgccgcg gcgacgatgg
     2701 gacggaactt cctctgcagc atgaccgttt cctcctggat tcctgcgcga atggttggta
     2761 tcggacgccc ggcttcgacg agtcgggcgt ctcgaaccac cgtgttcgga gcaggcgagt
     2821 gggcggtttc agttcgtgcc gcggaggggg gcgggccggc gcggccgcgg gacttcgacg
     2881 tgggggcggg ccacgatcac ctggcgcggc cgaacacgtc gagcggcact cgcaggtagc
     2941 ggacgccgtc ctgctccggc ggcggcaggt gtccggcacg tatgttcacc tgtacagccg
     3001 ccacgatcag gttcggcacc tcgagcgtgg cgtcgcgttc cgtgcgcagg cgcacgaacg
     3061 cggcctcgtc gacgccgtcg cgcaagtgca cgttcgtgcg ccgctgctcg gcgacgctcg
     3121 tctcgcagcg cgcctcgcgc ccgcccgggc ggtagtcgtg gcagacgtaa acgcgcgtcg
     3181 ccggcggcag cgcgtacagc cggtggacga tggagtggta caactggcgc gcgtcgccac
     3241 cgggaaaatc gcagcgcgcc gatccgctgt cgggcatgaa cagcgtgtcg ccgatgaagg
     3301 ccgcgtcgcc cgcgacgaag ctgacactgt cgctggtgtg gccgggcgtg gcgagcacgc
     3361 ggatcgtcat cgatccaagt ggcaggctct cgccgtcgtc gagcagccgg tcgaactggc
     3421 ggccgtcggg ttcgaagtcg tgtccgaggc catagatgcg cttgaacgtg cgctgtacgt
     3481 cgaggatgcc gcggccgatc gcgacctcac ccgccaggcg tcccttgagg tgctgggcgg
     3541 ccgagaggtg gtcggcgtgc gcatgcgtct ccaggatcca gcggacgtcg agcccctgcc
     3601 tttggcagtg ctcgaccacc tcgtcggccg cggtggtggc cgtgcgcacc gacttccagt
     3661 cgaagtcgag caccgggtcg acgatcgccg ccgccccgga tcgcgggtcc gcgatcacgt
     3721 acgtccagga gccggtgccg gcgtggtgaa agcccttgac gtcggggtgc atgtgcgacg
     3781 ggctcccggg gcgctcgttc agcgtgtcat cggcccgcgt cgaggcgcgt cggtgccgag
     3841 cgatcgcccg gtcagcggcg cacccatcgc ggccagcacc gcgcgttgct gcgcgatcgg
     3901 tgtacgcaac tggcgctctt cgaacggccg gaacttcgcg aaggcctcgt gcgcgagcac
     3961 gcggtcgacg aaggcggcgg tgcgcggcca gcgttcggca tcgacgcgtt gccgtgcgaa
     4021 gcccaggttg cggaacggcg ccgccagcgc gatgtcggcg atgccgatcg tcgccggatc
     4081 gaacaggaat ccggtatcgg gcacctgcga ctcgaggtag tcgagcacgc ccggtaactc
     4141 gtcctgcatc acgcgctcga tcgctgcgag gtcgcgaggg cggccccaga ttccgggatc
     4201 gagcaccgcc ttgttgaaga cgccccagac gaccacgtcg gcgaggcggg tgtcggcgta
     4261 ctcctcgagc cagcgcgcgc gggcacggtc ggcgacgtcc gccgggtaca gcgcatgacg
     4321 ttcggggtat cgatcttcga ggtattgcac gatcacggtc gaatcgcaca gcgtgactcg
     4381 atcgtcgatc aggatcggca cgcggcgcag cgggctcagc cgcgagtatt cgtcgtcacc
     4441 gtagaacgga gcgatcgggt cgatgcggta gtcgagctgc ttgagctcga ggcacgcgag
     4501 caccttgcgc acgaacggcg agacgtagct gccgacgacg gtgatgctgc cctcacccgc
     4561 gctgcgcgtg acacctcccg caggggcggg agagccgttc atgtctctcc cgtggccggt
     4621 ttctcgagct cagccagccg gcgttcgagc gcgttgagct tttctcgcgt gcgcttcaga
     4681 accg
//
LOCUS       DASPFR010000061         4363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_20614, whole genome shotgun sequence.
ACCESSION   DASPFR010000061 DASPFR010000000
VERSION     DASPFR010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20614"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VD737_01500"
     CDS             <1..916
                     /locus_tag="VD737_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HXW09265.1"
                     /translation="ARYTGLAIGATAAGPVLYAADFANGKVDVFDGAFAKQSVPATAF
                     VDPQLPANYAPFGIQAFPNGPGGATQVYVSYARKDALGSVEPGAGLGLVDVYDANGAL
                     LTRLVAEGGQLDAPWGMALAPDDFGTLGGALLVGNFGDGRIHGYDPVNGQLVGIVANV
                     VREPFALPGLRGLVFGNDAHNLPHSTLFYAAGTNDGLNGAVGRIDLGEVPPVLNAPPV
                     VAIASPPAGSVSGVVTVAASVTASTNVASVEFFAGTTLLGIVGAPPYSIAWDTTTRPN
                     GAVTLTARATDTSGNVGTSPPVTVTVSN"
     gene            947..1378
                     /locus_tag="VD737_01505"
     CDS             947..1378
                     /locus_tag="VD737_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09266.1"
                     /translation="MTESNTNPLGTSTGTETGGDYASGTAATTPGFQGTRARPEIMVD
                     TNAEEAYWRQNYVNRPYVTEGTSFTEYKPAYRYGVEAHTRHPGRSFDEAEAELSQDWD
                     RFKGTSSLTWDQAKLAARDAWHRVKDFVERATPGDADRDGK"
     gene            1460..4213
                     /gene="ppc"
                     /locus_tag="VD737_01510"
     CDS             1460..4213
                     /gene="ppc"
                     /locus_tag="VD737_01510"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HXW09267.1"
                     /translation="MEIDDEPDRPMRDDIRLLGRLLGDTVREQQGAPAFELVERVRQS
                     SIAFRRDDDVEARRELERILDGLSREQTMTVVRAFSYFAHLANLAEDVHNGRRRRQAE
                     IAGDPAADGTLARALDRLEAAGVPADAVDAFFRDGLMVPVLTAHPTEVQRKSVRDLEL
                     KVERLLHARERWRLTPDERAANDESLRRAVLALWQTRMLRLKKLDVLDEVANGLSFFE
                     HTFLPELPRLYAALEDELARRRVDATPAELPSMLRVGSWIGGDRDGNPYVTADVLERT
                     LRMQSASAFEFYLEQLHTLGAELSVTTLVSGTSPELMALAERSPDQSPHRRDEPYRRA
                     LTGIYARVAATARALDQLEPLRHAIAEAPPYARAAEFAADLQVMHASLVSHGSSLIAR
                     GRLRRLRRAVAVFGFHLAAVDLRQNSEVHERVVAELFSVARPDVEYLRLDEAARVQVL
                     VDELATPRLLSSPYVEYSSETASELAILRTAAAMHARYGAGCIENYVISKCDGASDVL
                     EVVLLLREVGLFRAADADLACHVVPLFETIADLRSAAGTMEQLLSIPVYARMLESRGR
                     SQEVMLGYSDSNKDGGYLTSRWELFKAQVALVETFERHGVRLRLFHGRGGSVGRGGGP
                     TYEAILAQPGGTVQGQIRITEQGEVIGVKYSHAEVGRQNLEILAAATLEATLLGRAGP
                     APKPEYLDALEALSARAFEAYRDLVYATPGFEDYFWESTVITEIAELNIGSRPASRKQ
                     SRSIETLRAIPWVFSWAQCRLMLPGWYGFGAAVDAWLAERGDEGLSLLREMHREWPFF
                     ASVLSNMEMVLAKADLAVASRYAGLVADEALRTSIFARIRAEFESSVAALLGIMGTDE
                     MLAHNPQLARSIKYRFPYLDPLNHIQVELLRRFRAGDRDERSRRGIHLTINGIAAGLR
                     NTG"
BASE COUNT          648 a         1577 c         1518 g          620 t
ORIGIN      
        1 cgcccggtac accggccttg cgatcggcgc gaccgccgcc gggccggtgc tgtacgcggc
       61 cgacttcgcg aacggcaagg tcgacgtgtt cgacggcgcg tttgcgaagc agtccgtccc
      121 ggccaccgcg ttcgtcgatc cgcagctgcc ggccaactat gcgccgttcg gcatccaggc
      181 gtttccgaac ggtcccggcg gcgcgacgca ggtctacgtg agctacgcgc gcaaggacgc
      241 gctaggcagc gtcgagcccg gcgccgggct cgggctcgtc gacgtctacg atgcgaacgg
      301 ggcgctgctc acccgactgg tggcagaggg cggccagctc gacgcgccct ggggcatggc
      361 actcgcgcct gacgacttcg gcacgctggg cggcgcgttg ctggtcggca acttcggcga
      421 cggccggatc cacggctacg acccggtcaa cggccagctc gtcggcatcg tcgccaacgt
      481 cgtgcgcgag cctttcgcgt tgccgggact gcgcgggctc gtcttcggca acgacgccca
      541 caacctgccg cactcgacgc tgttctacgc ggcgggcacc aacgacggcc tgaacggcgc
      601 ggtcggacga atcgacctgg gcgaggttcc gccggtgctc aacgcgccgc cggtcgtcgc
      661 gatcgcgtcg ccgccggcgg gcagcgtgtc gggcgtcgtg acggtggcgg ccagcgtgac
      721 tgccagcacc aacgtcgcca gcgtcgaatt cttcgcgggc accaccttgc tcggcatcgt
      781 cggcgcaccg ccctactcca tcgcctggga cacgacgacc cggcccaacg gcgcggtcac
      841 cctgaccgcg cgagcgacgg acacgagcgg caacgtcggc acctcgccgc cggtgaccgt
      901 gaccgtctcg aactgaacga acgcactcac cacggagatc gcatcgatga ccgaaagcaa
      961 caccaatccc ctcgggacat ccacgggcac ggagaccggc ggcgactacg ccagtggcac
     1021 cgccgcgacg acaccgggtt tccagggtac gcgcgcgcgc cccgaaatca tggtcgacac
     1081 caatgccgag gaagcctact ggcggcagaa ctacgtgaac cgcccgtacg tgaccgaagg
     1141 cacgtccttc accgaataca agccggccta tcgctatggc gtcgaagcgc acacgcgcca
     1201 tccgggccga tcgttcgacg aggccgaagc cgagctcagc caggactggg accgcttcaa
     1261 gggcacctcg agcctgacct gggaccaggc gaagctcgcc gcgcgcgacg cgtggcaccg
     1321 cgtcaaggac ttcgtggagc gagcgacgcc cggcgacgcc gaccgcgacg gaaagtaatg
     1381 tgacgatgcc caccgcgcag tagccggtgg ggccgccgtc ctctatcgtg cccgggccgt
     1441 ctgcaacccg ggagccgcgg tggagatcga cgacgagccc gaccgaccga tgcgcgatga
     1501 catccggctg ctcggccggc tgctcggcga caccgtgcgc gagcagcagg gcgcgccggc
     1561 gttcgagctg gtcgagcgcg tgcggcagag ctcgatcgcg ttccgccgcg acgacgacgt
     1621 ggaggcgcgt cgcgaactcg agcgcatcct cgacggcctc tcgcgcgagc agaccatgac
     1681 ggtggtgcgc gcgttcagct atttcgcgca cctggccaac ctcgccgagg acgtacacaa
     1741 cggccgacgg cgccggcagg cggagatcgc cggcgacccc gccgccgacg gcacgctcgc
     1801 gcgcgcgctc gaccgcctcg aggctgccgg ggtccccgcc gacgccgtgg acgcgttctt
     1861 ccgcgacgga ctgatggtgc cggtgctcac cgcgcacccg accgaggttc agcgcaagag
     1921 cgtccgcgac ctcgagctca aggtcgagcg cctgctgcac gcgcgcgaac gctggcgcct
     1981 gacccccgac gagcgtgcgg ccaacgacga atcgctgcgc cgcgccgtgc tcgcgctctg
     2041 gcagacgcgc atgctgcggc tcaagaagct cgacgtgctc gacgaggtcg cgaacgggct
     2101 gtcgttcttc gagcacacgt tcctgccgga gctgccacgc ctctacgcgg cgctcgagga
     2161 cgagctcgca cgacgtcggg tcgacgccac cccggccgag ttgccttcga tgctgcgggt
     2221 cgggtcgtgg atcggcggtg atcgcgacgg caatccgtac gtgaccgccg acgtgctcga
     2281 gcgcacgctg cgcatgcagt cggcgagcgc gttcgagttc tacctggagc agctgcacac
     2341 gctcggcgcc gagctgtcgg tgacgacgct cgtgagcggc acctcgccgg aactcatggc
     2401 gctcgccgag cgctcgcccg accagagccc gcaccggcgc gacgagccct atcgccgcgc
     2461 cctcaccggc atctacgcgc gcgtcgccgc gaccgcccgc gcgctcgacc agctcgagcc
     2521 gcttcgtcac gcgatcgccg aggcgccgcc atatgcgcgc gcggcggagt tcgcggccga
     2581 cctgcaggtg atgcacgcct cgctggtgtc gcacggttcg agcctgatcg cgcggggccg
     2641 cctgcgccgc ctgcggcgcg cggtcgccgt gttcggcttc cacctggccg ccgtcgacct
     2701 gcgccagaac tccgaggtgc acgagcgcgt cgtggcggaa ctgttctcgg tcgctcgtcc
     2761 ggacgtggag tacctgcgcc tcgacgaggc ggcccgcgtg caggtgctcg tcgacgaact
     2821 cgccacgccg cggctgctga gctcgcccta cgtcgaatac tcgagcgaga cggcgagcga
     2881 actggcgatc ctgcgcaccg ccgccgccat gcacgcgcgc tacggggccg ggtgcatcga
     2941 gaactacgtg atctcgaagt gcgacggggc ctccgacgtg ctcgaggtgg tgctgctgct
     3001 gcgcgaggtc gggctgttcc gggccgcgga cgccgacctc gcctgccacg tcgtgccgct
     3061 gttcgagacg atcgcggacc tgcgcagcgc cgccgggacg atggagcaat tgctctcgat
     3121 ccccgtctac gcgcgcatgc tcgaatcgcg gggtcgctcg caggaagtga tgctcggcta
     3181 ttccgacagc aacaaggacg gcggctatct cacctcgcgc tgggagctgt tcaaggcgca
     3241 ggtggcgctc gtcgagacgt tcgagcggca cggcgtgcga ctgcgcctgt tccacggccg
     3301 gggtggctcg gtcggccgcg gcggcgggcc cacgtacgag gccatcctcg cgcagcccgg
     3361 cggcacggtg caggggcaga tccgcatcac cgaacagggc gaggtgatcg gcgtgaagta
     3421 ctcgcacgcc gaggtcggcc gccagaacct cgagatcctc gcggccgcga cgctcgaggc
     3481 gacgctgctc ggccgggcgg gtccggcgcc gaaacccgag tacctcgacg cgctcgaggc
     3541 gctgtcggca cgggccttcg aggcttaccg cgacctcgtg tacgcgaccc ccggcttcga
     3601 ggactacttc tgggaatcga cggtgatcac cgagatcgcc gagctcaaca tcggcagccg
     3661 gcccgcctcg cgcaagcaat cgcgctcgat cgagacgctg cgcgcgatcc cctgggtgtt
     3721 ctcctgggcg cagtgccggc tgatgctgcc gggctggtac ggcttcggcg cggccgtgga
     3781 tgcgtggctc gccgagcgcg gcgacgaagg cctgtcattg ctgcgcgaga tgcaccgtga
     3841 gtggccgttc ttcgcgtccg tgctctccaa catggaaatg gtgctcgcga aggccgacct
     3901 cgcggtggcg tcgcgctacg ccgggctcgt cgccgacgag gcgttgcgga cgtcgatctt
     3961 cgcgcgcatc cgcgccgagt tcgagtcgag cgtcgcggcc ctgctcggca tcatgggcac
     4021 cgacgagatg ctggcgcaca acccgcagct cgcccgctcg atcaagtacc gcttcccgta
     4081 cctcgacccg ctcaaccaca tccaggtcga gctgctgcga cggttccggg cgggagaccg
     4141 cgacgagcgc agccgccgcg ggatccacct gacgatcaac ggcatcgcgg cgggactgcg
     4201 caacacgggt tgatcacttc tcgcgcttgc gcgggagagg tcgcgcgtag cgcgggtgag
     4261 cgcctcttca cctctcccgc gcttgcggga gaggtcgcgc gcagcgcggg tgagggcctc
     4321 ttcacctctc ccgcgcttgc gggagacgtc gcgcgcagcg cgg
//
LOCUS       DASPFR010000062         5439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1486360, whole genome shotgun sequence.
ACCESSION   DASPFR010000062 DASPFR010000000
VERSION     DASPFR010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486360"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VD737_01515"
     CDS             complement(<1..279)
                     /locus_tag="VD737_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HXW09268.1"
                     /translation="MQVPLPPPGSAEEGWHEREEAIMGTRIAVQAWGPRAQVVQAIDA
                     VMADMHRTDELMSTYKPWSQLSQVNAHAFERPVQVDPDIVDLLAIALEY"
     gene            complement(279..1442)
                     /locus_tag="VD737_01520"
     CDS             complement(279..1442)
                     /locus_tag="VD737_01520"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXW09269.1"
                     /translation="MLAHLGRQAAQYRSALTDDVVRASTRNRLKLLAHACDDHARRLR
                     ALLAPLAIGDDAANPATYAALDPGVAVGPGIAGYYANVHRDWCWGDVENEATFGAVQA
                     ALGGHTPKSIAVLGAGAGRLAYDLHERLRPGRTIAADVSPLLTFVARRMFEGERLELY
                     EFPVAPRDLASHALLRTLAAPRRARPGLHAVFGDARQAPLAPGAFDTVITPWLVDVID
                     ADLEQVAAAVNALLEPGGRWVCTGTMFFERRDPAHAYSSEEVEEVVAATGFSRPTLAA
                     RRVPYLASPASRHARDEELVTFAVTRVAESPARRPGPAAWLDDPRRAVPLPPPVAERT
                     LALRVEGYVASLVDGRRSLADIATRLVDERLLMPDEALGVVRNYVVRLFQESR"
     gene            complement(1642..3276)
                     /locus_tag="VD737_01525"
     CDS             complement(1642..3276)
                     /locus_tag="VD737_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09270.1"
                     /translation="MLIRHRALRTLRPDEPLKHADAHKRPVTRRDFIAQGLSAGLATV
                     VAPTVFSLFANPRAAMAALSPDLQALKSSCGIDVLGAGKIPFICLDLAGGANMAGSNV
                     LIGGPGGQLDFLSTAGYGKLGLPGDMLPGMVNPATGTSFTDSRLGLAFHSDSAFLRGI
                     LDRVSPATAAAVNGAVIAARSENDTGNNPHNPMYGIHQAGADGSLATLIGSRASDSGG
                     NSMAPAGMIDLSVRPTKVDRASDVTGLVDTGQLASLLSLDDTVAVMEAIQRISDRKLA
                     VVDPKQPWLDPKQTTVTRNEVVKELVSCGYVKAADVVDRFGDPSSLDPELDPDIVGAG
                     GIFSRAEYDGDAEFRKTAAVMKMVLSGYAGAGTITMGGYDYHGQGRSTGELRDLRAGR
                     CMGACLEYAARRGLPLMLYVFSDGSISASGQVDDSPDGRGKFMWTSDNQSTAASFFLV
                     YNPGGRPSLLTTGPGGAARHQQIGYFRADASVETASSPAANNVNLLVETVVLNYMALH
                     GEQGQFGTRFPRHGLGNATLQDSLIAFNPIVSGRIG"
     gene            complement(3299..>5439)
                     /locus_tag="VD737_01530"
     CDS             complement(3299..>5439)
                     /locus_tag="VD737_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HXW09271.1"
                     /translation="GSPRDSRLVQKVAGGHNCWLASNDACADILTTWVTAWAGAAAGG
                     AQGIVLKAPVLKDPGASKGFPTSPALFSTTVYPLLEQHCSRCHTSTAAASQSPFFASG
                     DVDEAYAAARVKIDLDQPALSRLVVRLRSEFHNCWSDCASNSSTMENAIVAFANQVPT
                     TQVDASLVHSKALRLPDGIVAAGGNRYTANTIGLWEFKTGRGTVAYDTSGIEPALNLN
                     LSGDVQWVGGWGINVRSGKAQGSTTASRKLHDLIQATGEYTIEAWVAPGNVGQEDARI
                     VSYSGGTTTRNFTLGQTLYSYDFLNRSTVTNLNGDPKLTTAAADEDLQATLQHVVATY
                     DPVQGRRLYVNGRFTDDVDPASGGTLADWDDTFAFVLGNEVSGDRQFQGVFRLVAVYN
                     RALTEPQIQTNFQAGVGEKFYLLFSVSHLVGVPEAYILFQVSQFDSYGYLFEKPTFIS
                     LDASATPGSIPIAGLRIGVNGEEAAVGQAYRTLDTTITDSQYGPLGQPLATIGTVIAL
                     EKGPEADEFFLTFDVLGSHRNVRLDPVPLAPGAPPDVARAPDVGLRVFDEINATMSEL
                     TGVSPTAPGVASTFATIRQSLPTVETLDTFVSAHPVAIAQLSIQYCNALVESGTLRAQ
                     YFPGFDFDAAPATAFAPAGRQILLDSLIARMLRASVPTEPSAAGVRSDLDALVTRLTS
                     CGAGCPTDRTRTVVKASCAALLGSAATLVH"
BASE COUNT          778 a         1878 c         1963 g          820 t
ORIGIN      
        1 atactcgagc gcgatcgcga gcaggtcgac gatgtccggg tcgacctgta ccggccgctc
       61 gaacgcgtgc gcgttgacct gggacagctg gctccagggc ttgtacgtgc tcatgagttc
      121 gtcggtgcgg tgcatgtccg ccatgacggc atcgatcgcc tggacgacct gcgcgcgcgg
      181 gccccaggcc tgcaccgcga tgcgggtgcc catgatcgct tcctcgcgct cgtgccagcc
      241 ctcctccgcg gaccccggcg gcggtaacgg cacctgcatc agcgcgactc ctggaacagc
      301 cgcaccacgt agttgcgcac gacgccgagc gcctcgtccg gcatcaacag gcgctcgtcg
      361 accagccgcg tcgcgatgtc ggcgagcgaa cgacggccgt cgacgagcga ggccacgtag
      421 ccctcgacgc gcagcgccag cgtgcgctcc gcgaccggcg gcggcagcgg gaccgcacgg
      481 cgcgggtcgt cgagccaggc cgccggcccg ggacgccgtg cagggctctc agcgacgcgc
      541 gtcacggcga acgtgaccag ttcctcgtcg cgcgcatggc ggctcgccgg cgacgccagg
      601 tacggcacgc gtcgggctgc cagagtgggc cgggagaacc ccgtcgccgc gacgacctcc
      661 tcgacctcct cgctcgagta ggcgtgcgcc gggtcgcggc gctcgaagaa catcgtgcct
      721 gtgcagaccc agcgaccgcc cggctcgagc agcgcgttga cggccgcggc gacctgctcg
      781 aggtccgcat cgatcacgtc gacgagccac ggcgtgatca cggtgtcgaa tgcgccgggc
      841 gcgagcgggg cttgcctcgc gtcgccgaac acggcgtgca ggccgggccg cgcgcgtcgg
      901 ggcgcggcga gcgtgcgcag caaggcgtgg cttgcgaggt cgcgtggcgc gaccgggaat
      961 tcgtagagct cgagccgttc gccttcgaac atgcgccgcg cgacgaacgt gagcaacgga
     1021 ctgacgtcgg cggcgatcgt ccgcccgggc cggagccgct cgtgcaggtc gtacgccagc
     1081 cggccggccc ccgcccccag cacggcgatg cttttcggtg tgtgcccgcc gagcgcggcc
     1141 tggaccgcgc cgaacgtagc ttcgttctcg acgtcgcccc agcaccagtc ccggtgcacg
     1201 ttggcgtaat agcccgcgat gcccggtccc accgcgacgc ctgggtcgag ggccgcatac
     1261 gtcgccgggt tcgccgcgtc gtcgccaatg gcgagcggcg ccagcagcgc acgcaggcgt
     1321 cgagcgtggt cgtcgcaggc gtgggccagc agcttgagcc ggttgcgcgt gctggcgcgg
     1381 acgacgtcgt ccgtcaaggc gctgcggtat tgcgccgcct ggcgcccgag gtgtgcgagc
     1441 aatgcgcggg cacgaccacg ccactcgtcg agtgcagcgg cgggttcggg aaacagccag
     1501 ggaatgccat cgaggcgcgg gtacgtcgca tcacacccgc cgcaggcgag cgccgccgac
     1561 gcctgcagag cggcgccaca gcgcggacac cggagtagat cgtggaccgt gctgtcgggt
     1621 gtcgtgctca accccgccag attagccgat ccggccgctc acgatgggat tgaacgcgat
     1681 caggctgtcc tgcagcgtcg cgttgccgag cccgtggcgc gggaacctcg tcccgaactg
     1741 gccctgttcg ccgtgcagtg ccatgtagtt gagcacgacg gtctcgacca gcaggttcac
     1801 gttgttggcc gccggactcg aggccgtctc gaccgatgcg tccgcgcgga agtagccgat
     1861 ctgctggtga cgcgccgcgc cgccggggcc ggtggtgagc aggctcggcc gtccgccggg
     1921 gttgtacacc aggaagaacg acgcggccgt cgactggttg tcgctggtcc acatgaactt
     1981 gccccggccg tctgggctgt cgtcgacttg cccgctcgcg gagatcgagc cgtccgagaa
     2041 cacgtagagc atcagcggca agccgcgccg cgcggcgtac tcgagacacg cacccatgca
     2101 tcggcccgcg cgtagatcgc gcagttcgcc ggtcgaccgg ccctgcccgt gatagtcgta
     2161 cccgcccatg gtgatcgtgc ccgcgccggc gtaacccgac agcaccatct tcatgacggc
     2221 ggccgtcttg cggaactcgg cgtcgccgtc gtactcggcg cggctgaaga tcccgccggc
     2281 gccgacgatg tcgggatcga gctcggggtc caggctggac gggtcgccga agcggtcgac
     2341 gacgtcggcc gccttgacgt agccgcagct gacgagctcc ttgacgacct cgttacgcgt
     2401 gaccgtggtc tgcttcggat cgagccacgg ctgcttgggg tcgacgaccg cgagcttgcg
     2461 gtcactgatg cgctggatcg cctccatgac cgcgaccgtg tcgtcgaggc tcagcaagga
     2521 tgccaactgg ccggtgtcca cgaggcccgt cacgtcgctc gcgcggtcca ccttggtcgg
     2581 tcgcaccgag aggtcgatca tgccggccgg cgccatcgag ttgccgcccg agtcggaggc
     2641 ccgcgatccg atcagcgttg ccagcgaccc gtccgcgccg gcctggtgga tgccgtacat
     2701 cgggttgtgc gggttgttgc cggtgtcgtt ctcggacctc gccgcgatca cggcgccgtt
     2761 gaccgccgcc gcggtggccg gcgagacacg atcgaggatg ccgcgcagga acgcgctgtc
     2821 gctgtggaac gcgagcccga gccgcgaatc cgtgaagctc gtgccggtcg ccgggttgac
     2881 catgccgggg agcatgtcgc cgggcagccc gagcttgccg tagccggccg tcgacaggaa
     2941 atcgagctgc ccgccgggtc cgccgatcag cacgttcgac ccggccatgt ttgcgccacc
     3001 ggcgaggtcg aggcagatga acgggatctt gccggcgccc agcacgtcga tgccgcaact
     3061 cgacttgagc gcctgcaagt ccggcgacag cgcggccatc gccgcgcgcg ggttcgcgaa
     3121 caggctgaag acggtcgggg ccacgacggt cgccagaccg gcagacaggc cctgcgcgat
     3181 gaaatcgcgc cgggtcacgg ggcgcttgtg ggcgtcggcg tgcttgagcg gctcgtcggg
     3241 gcgcagcgtg cgcagggctc gatgacggat cagcatgacg tgactccggg gcggcgcatc
     3301 agtggaccag cgtcgccgcg ctgccgagca gcgccgcgca actcgccttg acgacggtcc
     3361 gcgtgcggtc ggtcgggcag cccgcgccgc agctcgtcag gcgcgtgacc agcgcgtcga
     3421 ggtcgctgcg cacgccggcc gcgctgggct ccgtgggcac gctcgcgcgc agcatgcggg
     3481 cgatgagcga gtcgagcagg atctgccgtc ccgccggcgc gaacgccgtc gccggggcgg
     3541 catcgaagtc gaatcccggg aagtactgcg cgcgcagcgt gccgctctcg accagcgcgt
     3601 tgcagtactg gatcgacagt tgcgcgatcg ccaccgggtg cgccgacacg aacgtgtcga
     3661 gcgtctcgac cgtcggtagc gattgcctga tcgtggcgaa cgtcgaggcg acgccaggtg
     3721 cggtcgggct gacgccggtc agctcggaca tcgtcgcgtt gatctcgtcg aacacgcgca
     3781 ggccgacgtc gggcgcgcgc gcgacgtcgg gcggggcccc gggcgcgagc ggcaccgggt
     3841 cgagccgcac gttgcggtgg ctgccgagca cgtcgaaggt caggaagaac tcgtcggcct
     3901 cgggtccctt ctcgagcgcg atcacggtac cgatcgtcgc gaggggctgg ccgagcggcc
     3961 cgtactggga gtcggtgatc gtggtgtcga gcgtgcgata ggcctgcccc accgcggcct
     4021 cctcgccgtt cacgccgatg cgcaggcccg cgatcgggat cgaacccggc gtcgcgctcg
     4081 cgtcgaggct gatgaaggtg ggcttctcga acaggtagcc gtacgagtcg aactggctca
     4141 cctggaacag gatgtacgct tcgggcactc cgacgaggtg gctgaccgag aacaggaggt
     4201 agaacttctc gccgacgccg gcctggaagt tcgtctggat ctgcggctcg gtgagcgcac
     4261 ggttgtagac ggccacgagg cggaacacgc cctggaactg gcggtcgccc gacacctcgt
     4321 tgccgagcac gaacgcgaac gtgtcgtccc agtcggcgag cgtgccgccc gacgccgggt
     4381 cgacgtcgtc ggtgaaccgt ccgttgacgt acaggcgccg gccctggacg gggtcgtacg
     4441 tcgcgacgac gtgctgcaac gtcgcctgca ggtcctcgtc cgccgccgcc gtcgtcagct
     4501 tgggatcacc gttcaggttg gtgaccgtcg agcggttcag gaagtcgtag ctgtacagcg
     4561 tctgcccgag cgtgaagttg cgcgtcgtcg tgccgccgga atacgagacg atgcgcgcgt
     4621 cttcctgtcc gacgttgccg ggcgccaccc acgcctcgat cgtgtactcg ccggtcgcct
     4681 ggatcaggtc gtgcagcttg cggctcgcgg tcgtcgagcc ctgcgccttg ccgctgcgca
     4741 cgttgatacc ccaaccgccg acccactgca cgtcgccgga gaggttcagg ttgagcgccg
     4801 gctctatgcc gctcgtgtcg tacgcgacgg tgccgcggcc ggtcttgaac tcccagaggc
     4861 cgatcgtgtt ggccgtgtag cgattgccgc cggccgcgac gatgccgtcg ggcagccgca
     4921 acgccttcga gtggacgagc gacgcatcca cctgcgtcgt cggcacctgg ttcgcgaacg
     4981 cgacgatggc gttctccatc gtgctcgagt tcgaggcgca gtcgctccag cagttgtgga
     5041 attcgctgcg caggcgcacg accaggcgcg agagcgccgg ctggtccagg tcgatcttga
     5101 ccctggcggc cgcgtacgcc tcgtcgacgt caccggaggc gaagaacggc gactgcgacg
     5161 cggccgcggt ggacgtgtgg caccgcgagc agtgctgctc gagcagcggg tacacggtgg
     5221 tcgagaacag cgccggcgac gtcgggaagc ccttgctggc ccccgggtcc ttgagcaccg
     5281 gtgccttgag cacgatccct tgcgcaccgc ccgcggcagc cccggcccag gccgtgaccc
     5341 acgtcgtgag gatgtcggcg cacgcatcgt tgctcgcgag ccagcagttg tgcccccccg
     5401 cgactttctg gacgagccgc gagtcgcgcg gcgagccga
//
LOCUS       DASPFR010000063         8760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_265238, whole genome shotgun sequence.
ACCESSION   DASPFR010000063 DASPFR010000000
VERSION     DASPFR010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8760
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265238"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VD737_01535"
     CDS             <1..303
                     /locus_tag="VD737_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09272.1"
                     /translation="RVNIVYERRQAALQLPRAAILDADGQQSVYVVSDGKAQQRVIRT
                     GLTSAGWIEVLDGLQGDERVVTVGQAGLKSGTPVKIVEDKPQPAAALASEKPKATK"
     gene            355..3600
                     /locus_tag="VD737_01540"
     CDS             355..3600
                     /locus_tag="VD737_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXW09273.1"
                     /translation="MLKALIDFAIERRVTIVMFTVAIALFGFVSLSRLKLNLLPDLSY
                     PTITIRTELPGAAPLEVETLITRPVEEAVSIIRNVREVRSVSRAGQSDVTLEFLWGTD
                     MDLSGIDVREKLDLLQLPLEARRPLLLRFDPSSEPVLRLALLDEKAQGTDGSVDRLKA
                     LRRLAEDRLKPDLEAVEGSAAVKVSGGYEDEVQVFVDQQKLAQLGLSIDVVTRRIRAE
                     NVNLSGGRLEQGTQRFLVRTLNEFETVEQMANAIIATRGGQPIYLRDVANVTRGYKDR
                     EAITRVNGQEAIELAVYKEGDANTVQLADGVRARVKEIEKNLPEGTSIRQVYDQSTFI
                     SAAISEVKSAALIGGVLAILVLYAFLRDARATLISSIAIPVSVLGTFVLMYAFDLTLN
                     IMSLGGIALAVGMLVDNAVVVLESIVRNKEHGHSQLEAARLGTVEVAGAVFAATLTSV
                     AVFAPMVFITGIAGQLFKDQSLTVTFALAFSLLVALTLVPMLAAGRARAAATADPSIP
                     ARPPGRVGRGLRHARDGAATLGSWIARSCALALSPLVGVTQRVNQWADRKYPPAIRWA
                     LAHPGQTIGGAVALFALTMLLVLPRLGTELIPQMSQGEFNVDLRLPPGTPLEQTDRSV
                     LAAQRASSALGNISLAYSVAGTGNRLDANPVDAGENTGRLSITLAPGSDRADEQAAMA
                     GMRTELEDLPGVQYRFSRPALFSMATPLEVVVAGYDLDRLGDAAERVRAEMLRDPRFA
                     DVKTTVEAGNPEIQIVFDQERASQLGLVVRDLADRVVNSVRGEVATRYKLRDKQIDVL
                     VRSVDSRAASIEEVRNLIVNPGSDFPVPLSAVADVHLAVGPAEVRRAGQERVAVVSAN
                     LAHGDLGSAVETLQAIVERVELPVGTTAFLSGQSEEMTASFKSLQFVLIAAIFLVYFV
                     MASQFESLIHPFVILLTIPLALIGAVWALWITGTTVNVVAYIGLIMLAGIVVNQSIVL
                     IDAVNQARARGLGKHDAIVEAGRLRLRPILITKLTTILGLLPMALGIGEGAEVRAPMA
                     ITVIGGVLLTTFLTLLVIPVVYSVMDRKKFTAVEPRPVLPPGDAAVEAVAG"
     gene            3622..6723
                     /locus_tag="VD737_01545"
     CDS             3622..6723
                     /locus_tag="VD737_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXW09274.1"
                     /translation="MLHTRVALHRPVTTVMVALAVLAVGLISSQLLRLEAMPDITFPG
                     MRVVIPYAGSTPEEMEELIVRPVEEALATLSGIEQIEASAQSDQAQFDIQFNWDRDAD
                     AAAFEVRTKLDSIRPQLPVGADRMLMFAFSAGDQPVAVIRISSDQDLTDQYDTLEKYL
                     KRPMERIDGVARVELEGVEPREVRILVDPTRLAAYSVNVVELRNLLEASNFSVSAGEI
                     TENNSRFTVRPIGEFRSLDDVRDLLVMPGVRLGDVAQVELVSPELDVGRHMDGRPAVG
                     IDVYKSTQANVVDVADRVLEAIEKAKELPQLQGIQILVVGNQAESIRESLKELRKAGL
                     IGAVLSFFMLLFFLRHFPTTVIVSLAVPASLLVTLGAMYFLGLTLNILTMMGMLLAIG
                     MLVDNSVVITESIFRHRQLHPGKPLESTLEGVREVGLATLAGTFSMIIVFLPLVFGEK
                     NQMSIFLVHVAVPIVVAMLASLVIAQTLLPMLAARMPPPPPIAGSSWFGRLQERYARA
                     LEWAMNHRKTMGAITLLLVVSPVPLFALKLAKVDPFPQEASRILMIAYHLEGSHPMAR
                     VEQAVNRIEAYIEANKERFDVDTYYTYWISDDAVTRLYLTPKAEARVPAAEVMERVLA
                     DMPEIIIGKPSFAFENGPGASTSFSLQLAGESTEQLADISRDVAHRLSSVEGLEAVRS
                     EAGTGEQEVQVIVNRDRASQLGLTTQDVAMTVAGAMRGDRLPELRTSERELTMRLAFR
                     ESDRQSVDDLSRIPLMLPDGSRIELGAVADFVVQPSDREIKRLNRLTTVIIDANLAEG
                     TTMDEARERVEPIMNDYPLPPGYTWKFGRGFEENDKAMQTMAQNMLLAVVLIFLVMAS
                     LFESALYPLSIITSIVFAIVGSIWFLALTGTTITMMAMIGFMVLIGVVVNIGIVLIAH
                     VIDLRHAGLPRHEAIIQAGRDRLRPIMMTTLTTLLGMLPLAVGDAQVGGAGDGSPAYY
                     PMARAIMGGLAFSSVVSLLIVPMFYVWFDDLNLWRQRVFGRQLQTAPGPVSGGPEPVV
                     PPA"
     gene            6905..7177
                     /locus_tag="VD737_01550"
     CDS             6905..7177
                     /locus_tag="VD737_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09275.1"
                     /translation="MAWLALGLPAVGRAQGVNALERLHLPDLSTLLVLGAGFGVVLIA
                     AGVLVFAIRQLRREAAERRRLYNRHRRGSHAHRPHVGPPRSPMTDR"
     gene            complement(7217..8695)
                     /locus_tag="VD737_01555"
     CDS             complement(7217..8695)
                     /locus_tag="VD737_01555"
                     /inference="COORDINATES: protein motif:HMM:NF021427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HXW09276.1"
                     /translation="MLLLGPLTPITCPSCEHEFSLADGFARHSLEALERASVGALEKL
                     QGEARAGEERRARERAAQGEALLRERLEGLQQMLDAQRRQHAESLAQMRALEQEGAAR
                     READLRASLEQRDAELREAAAERDALADRARDIARREASMAEAIEAGAASRAVELAAG
                     TRRELEERLASQQAQLQQSQALELQLRREREALEQQQRQLELDVQRRLDEERCRIADT
                     VRAAESERARLREAELQKKLDDTLDKLSDTQRRLEQGSQQLQGEVLELVLEEELAAAF
                     ALDVVSEVRKGVRGADASQRVMTRSGQQAGVILWEAKRAQKWSTQWTAKLREDLREAG
                     ADVGVIVTTCWPADWPEGQPFGLHDDVWVTGPGAAIALAGALRSGLLEAYKARVAAAN
                     RGEKMEAVYDYLVSPQFAHKLRAVYDAFKRMREELESERTTMQQRWKRRERQIALATN
                     QLIAIAGDLQGLAQQELPQLELEPKALFGDDEERALEHALEE"
BASE COUNT         1254 a         3122 c         2966 g         1418 t
ORIGIN      
        1 cgcgtcaaca tcgtctacga gcggcgccag gccgcgctgc agctgccgcg cgcggcgatc
       61 ctcgacgccg acgggcagca gtcggtctac gtcgtgtcgg acggcaaggc gcagcagcgc
      121 gtgatccgca ccggcctcac cagtgcgggg tggatcgaag tcctggacgg cctgcagggc
      181 gacgagcgcg tcgtcacggt cggccaggcc ggcctcaagt cgggcacgcc ggtcaagatc
      241 gtcgaggaca agccgcagcc tgcggccgcg ctcgcctccg agaagcccaa ggccaccaag
      301 taagtccgcc ggggcggccc ccgcgccgcc ccgccctgcg ccggggatcc gcccatgctc
      361 aaagcactga tcgatttcgc gatcgagcgc cgggtcacga tcgtgatgtt cacggtcgcg
      421 atcgcgctgt tcgggttcgt ctcgctgtcg cgactcaagc tcaacctgtt gccggacctg
      481 tcgtacccga ccatcacgat ccgcacggaa ttgccgggcg cggcgccgct cgaagtcgag
      541 acgctgatca cccgcccggt cgaggaagcc gtcagcatca tccgcaacgt ccgcgaagtg
      601 cggtcggtct cgcgcgccgg gcagtcggac gtgacgctcg agttcctctg gggcacggac
      661 atggacctct cgggcatcga cgtgcgcgag aagctcgacc tgctgcagtt gccgctcgag
      721 gcccggcggc cgctgctgct gcgcttcgat ccctccagcg aaccggtgct gcggctcgcg
      781 ctgctcgacg agaaggcgca gggcaccgac ggcagcgtcg accgcctgaa ggcgttgcgc
      841 cgcctcgccg aagaccggct gaagccggac ctggaggccg tcgagggctc ggccgccgtc
      901 aaggtgagcg gcggctacga ggacgaggtc caggtgttcg tcgaccagca gaagctcgcg
      961 cagctcgggc tctcgatcga cgtggtcacg cgccggatcc gcgccgagaa cgtcaacctg
     1021 tccggcggtc gtctcgagca gggcacccag cgctttctcg tgcgcacgct gaacgagttc
     1081 gagaccgtcg agcagatggc caacgcgatc atcgccacgc gcggcgggca gccgatctac
     1141 ctgcgcgacg tcgcgaacgt gacccgcggc tacaaggatc gcgaggcgat cacgcgcgtc
     1201 aacggccagg aggcgatcga gctcgcggtc tacaaggaag gcgatgccaa cacggtgcag
     1261 cttgccgacg gcgtgcgggc gcgcgtcaag gagatcgaga aaaacctgcc cgaaggcacc
     1321 tcgatccgcc aggtctacga ccagtcgacg ttcatcagcg cggcgatcag cgaggtcaag
     1381 agcgccgcct tgatcggcgg cgtgctggcg atcctggtgc tgtacgcgtt cctgcgcgat
     1441 gcgcgcgcga cgctgatctc gagcatcgcg atcccggtct cggtgctcgg cacgttcgtg
     1501 ctcatgtacg cgttcgacct gaccttgaac atcatgtcgc tcggtggcat cgcgctcgcc
     1561 gtcggcatgc tggtcgacaa cgccgtcgtc gtgctcgaga gcatcgtgcg caacaaggaa
     1621 cacggccact cgcagctcga ggccgcgcgc ctcggcaccg tcgaggtggc gggtgcggtg
     1681 tttgcggcca ccctgacgtc cgtcgcggtg ttcgcgccga tggtgttcat caccggcatc
     1741 gccggccagc tgttcaagga ccagtcgctc acggtcacgt tcgcactcgc cttctcgctg
     1801 ctggtggcac tgacgctggt gccgatgctc gcggccggcc gggcccgcgc cgcggccacc
     1861 gccgaccctt cgatcccggc caggccaccc ggccgcgtcg gccgcgggct gcggcacgct
     1921 cgcgacggag ccgcgacgct cggcagctgg atcgcgcgca gttgcgcgct cgcgctgagc
     1981 ccgctcgtcg gcgtgacgca gcgggtcaac cagtgggcgg accgcaaata tcctcccgcg
     2041 atccgctggg cgctcgcgca ccctggacag acgatcggcg gcgccgtcgc gctgttcgcg
     2101 ttgacgatgc tcctggtgct gccacgtctc ggcaccgagc tgatcccgca gatgtcgcag
     2161 ggtgagttca acgtcgacct gcggctgccc cccggcactc cgctcgagca gaccgatcga
     2221 tcggtgctcg cggcccagcg cgccagctcc gccctcggca acatctccct ggcctactcg
     2281 gtcgccggga caggcaaccg cctggacgcg aatccggtcg acgcgggcga gaacaccggc
     2341 cgcctgagca tcacgctggc gcccggttcg gaccgcgcag acgaacaggc cgcgatggcc
     2401 ggcatgcgca cggagctcga ggacctgccc ggcgtgcagt atcgcttcag ccggccggca
     2461 ctgttcagca tggcgacgcc gctcgaggtg gtggtcgccg gctacgacct cgaccggctc
     2521 ggcgacgccg ccgagcgcgt gcgcgccgaa atgctgcgcg atccccggtt cgccgacgtc
     2581 aagaccacgg tcgaggccgg caaccccgag atccagatcg tgttcgacca ggagcgcgcc
     2641 tcgcagctcg gcctcgtcgt gcgcgacctg gccgaccgcg tcgtcaacag cgtgcgcggc
     2701 gaggtcgcca cgcgctacaa gctgcgcgac aagcagatcg acgtgctggt ccgcagcgtg
     2761 gactcgcgcg cggcctcgat cgaggaggtg cgcaacctga tcgtcaaccc gggcagcgac
     2821 ttcccggtgc cgctctcggc cgtcgccgac gtccacctcg cggtcgggcc cgccgaagtg
     2881 cgccgcgcgg gccaggagcg cgtcgcggtg gtctcggcca acctcgccca cggtgacctg
     2941 ggcagcgccg tcgaaacgct gcaggcgatc gtcgagcgcg tcgagctgcc cgtcggcacg
     3001 acggcgttcc tctccggcca gagcgaggaa atgaccgcct cgttcaagtc gctgcagttc
     3061 gtgctgatcg cggcgatctt cctggtctac ttcgtgatgg cctcgcagtt cgagtcgctg
     3121 atccatccgt tcgtgatcct gctgaccatc ccgctcgcgc tgatcggcgc ggtctgggcg
     3181 ctgtggatca ccggcacgac cgtcaacgtc gtcgcctaca tcggcctgat catgctggcg
     3241 ggcatcgtgg tcaaccagtc gatcgtgctg atcgacgcgg tcaaccaggc gcgcgcacgc
     3301 gggctcggca agcacgacgc gatcgtcgag gccggccggc tgcggctccg accgatcctg
     3361 atcaccaagc tcacgaccat cctcggcctg ctgcccatgg cgctcggcat cggcgaaggc
     3421 gccgaggtcc gcgccccgat ggcgatcacg gtgatcggcg gcgtgctgct gacgacgttc
     3481 ctgacgctgc tcgtgatccc ggtggtgtac tcggtcatgg accgcaagaa gttcacggcg
     3541 gtcgagccgc ggccggtgct gccaccgggc gacgcggccg tggaagcggt cgcgggctga
     3601 ccgaagccag ggggagccac catgctgcac accagggtcg ccttgcaccg tcccgtcacc
     3661 accgtgatgg tggcgctcgc cgtgctggcc gtcggcctga tctcgagcca gctgctgcgg
     3721 ctcgaagcga tgcccgacat cacgttcccg ggcatgcgcg tcgtgatccc ctacgccggc
     3781 tcgacgccgg aggaaatgga agagctgatc gtgcgcccgg tcgaggaagc gctcgcgacg
     3841 ctctccggca tcgagcagat cgaggcgagc gcgcagtccg accaggccca gttcgacatc
     3901 cagttcaact gggaccggga cgccgacgcg gcggcgttcg aggtgcgcac caagctcgat
     3961 tcgatccggc cccagctgcc cgtcggcgcg gaccggatgc tgatgttcgc gttcagcgcc
     4021 ggggaccagc cggtggccgt gatccgcatc tcgtccgacc aggacctcac ggaccagtac
     4081 gacacgctcg agaaatacct gaagcgtccc atggagcgca tcgacggcgt cgcgcgcgtc
     4141 gagctcgagg gggtcgagcc gcgcgaggtc cgcatcctgg tcgacccgac gcggctcgcc
     4201 gcctacagcg tcaacgtcgt cgagctgcgc aacctgctcg aggccagcaa tttctcggtg
     4261 agcgccggcg agatcaccga gaacaactcg cgcttcacgg tgcgcccgat cggcgagttc
     4321 cgcagcctgg acgacgtgcg cgacctgctg gtgatgccgg gcgtgcgcct cggcgacgtc
     4381 gcgcaggtgg agctcgtgtc tcccgagctc gacgtcgggc ggcacatgga cggacgcccg
     4441 gccgtgggca tcgacgtcta caagtcgacg caggcgaacg tggtcgacgt ggcggaccgg
     4501 gtgctcgagg cgatcgagaa agccaaggag ctgccccagc tgcagggcat ccagatcctg
     4561 gtggtgggca accaggccga aagcatccgc gagtcgctca aggagctgcg caaggccggc
     4621 ctgatcggcg ccgtgctcag cttcttcatg ctgctgttct tcctcaggca cttcccgacg
     4681 acggtgatcg tgagcctggc cgtgccggcc tcgctgctcg tgacgctcgg ggccatgtac
     4741 ttcctcgggc tcacgctcaa catcctcacg atgatgggca tgctgctcgc catcggcatg
     4801 ctcgtggaca actcggtggt gatcaccgag agcatcttcc gccaccgcca gctgcacccc
     4861 ggcaagccgc tcgagtccac cctcgaaggc gtcagggaag tcggcctcgc gacgctcgcc
     4921 gggacgttct cgatgatcat cgtcttcctg ccgctcgtgt tcggcgagaa gaaccagatg
     4981 tcgatcttcc tggtgcacgt cgccgtgccg atcgtcgtcg cgatgctcgc ctcgctcgtg
     5041 atcgcgcaga cgctgctgcc gatgctcgcg gcccgcatgc ccccgccccc gccgatcgct
     5101 ggctcgtcgt ggttcggccg gctgcaggag cggtacgcgc gcgcgctcga gtgggccatg
     5161 aaccatcgca agaccatggg agcgatcacg ctgctgctgg tggtctcgcc cgttccgctc
     5221 ttcgcactca agctcgccaa ggtcgacccg ttcccgcagg aggcgagccg aatcctgatg
     5281 atcgcctatc acctcgaggg ctcgcacccg atggcgcgag tcgagcaggc cgtcaaccgc
     5341 atcgaggcct acatcgaggc caacaaggaa cgcttcgacg tcgacaccta ttacacgtat
     5401 tggatcagcg acgacgccgt cacccgtctg tacctcactc ccaaggccga ggccagggtg
     5461 ccggccgccg aggtcatgga gcgcgtgctc gccgacatgc ccgagatcat catcggcaag
     5521 ccgagcttcg cgttcgagaa cgggcccggc gcttcgacgt cgttcagcct gcagctcgcc
     5581 ggcgaatcga ccgagcagct cgcggacatc tcgcgcgacg tcgcccatcg cctgtcgtcg
     5641 gtcgagggcc tcgaggccgt gcggtcggag gccggcacgg gcgagcagga ggtgcaggtc
     5701 atcgtgaacc gcgaccgcgc ctcgcagctc ggcctgacga cccaggacgt ggcgatgacc
     5761 gtcgccggcg ccatgcgtgg cgatcgtctg ccggagctgc gcacgtcgga gcgcgagctg
     5821 accatgcgcc tcgcgttccg cgagtccgac cggcagtcgg tcgacgacct ctcgcgcatc
     5881 ccgctcatgt tgccggatgg ctcccgcatc gagctcggcg ccgtggcgga cttcgtcgtg
     5941 cagccgagcg accgcgagat caagcgcctc aaccgcctga cgaccgtgat catcgacgcg
     6001 aacctggccg aggggaccac gatggacgag gcccgcgagc gcgtcgagcc catcatgaac
     6061 gactatccgc tgccgcccgg ctacacgtgg aagttcggac gcgggttcga agagaacgac
     6121 aaggcgatgc agaccatggc gcagaacatg ctgctcgcgg tcgtgctgat cttcctggtg
     6181 atggcgtcgc tcttcgagtc ggcgctctat cccctctcga tcatcacctc gatcgtgttc
     6241 gcgatcgtgg gctcgatctg gttcctggca ctgacgggca cgaccatcac gatgatggcg
     6301 atgatcgggt tcatggtgct gatcggcgtc gtggtcaaca tcggcatcgt gctgatcgcg
     6361 cacgtgatcg acctgcgcca cgccggactg ccgcggcacg aggcgatcat ccaggccggt
     6421 cgcgaccgcc tgcggccgat catgatgacg acgttgacga cgctgctcgg catgctgccg
     6481 ctcgcggtcg gcgacgcgca ggtcggtggc gcgggcgacg gcagcccggc gtactacccg
     6541 atggcgcgcg ccatcatggg cggtctcgcg ttctcgtcgg tcgtctcgct gctgatcgtg
     6601 ccgatgttct acgtctggtt cgacgacctg aacctgtggc gccagcgcgt cttcggccgg
     6661 caactgcaaa ccgcccctgg accggtctcc ggcggaccag agcccgtcgt gcccccggcc
     6721 tgaggcaacg ctcgcctcgc ctcgctcgcg caccgcaagc cggcgcggcg cgaactatcc
     6781 ccgggcgtgg ctgtctccaa caggacgccc ttcgcgcgcc gcccttccca agtcgccatc
     6841 catggatcgg atcgaccgtc tccaccgccc acgggccggc tcgtcgtggc tgggcctttg
     6901 tgccatggcc tggctggcct tgggcctgcc tgccgtgggc cgagcccagg gggtcaatgc
     6961 gctcgagcgc ctgcacctgc ccgacctctc gaccctgctg gtgctcgggg ccgggttcgg
     7021 cgtggtactg atcgccgcgg gcgtgctcgt cttcgcgatc cggcaattgc gccgtgaagc
     7081 cgcggagcgg cgccggctct acaaccggca tcgacgcggc agccacgcgc accgtcccca
     7141 cgtcggcccg ccgaggtccc cgatgaccga ccgatgaccg attgacgcga cgtgagggtt
     7201 ccgtcgcggc gcgccgtcac tcctccagcg cgtgctcgag ggcgcgttcc tcgtcgtcac
     7261 cgaagagcgc cttcggttcg agttcgagtt gcggaagctc ctgctgtgcc aggccctgca
     7321 ggtcgccggc gatggcgatc agctggttcg tcgccagcgc gatctgcctt tcgcggcgct
     7381 tccagcgctg ctgcatggtc gtgcgctcgc tctcgagttc ttcgcgcatg cgcttgaacg
     7441 cgtcgtagac ggcccgcagc ttgtgggcga actgcggcga gaccaggtaa tcgtagaccg
     7501 cctccatctt ctcgccccgg ttggccgcgg cgacgcgcgc cttgtaggcc tcgagcagcc
     7561 cgctgcgcag cgcgcctgcc agcgcgatcg ccgcgcccgg cccggtgacc cagacgtcgt
     7621 cgtgcagtcc gaacggctgt ccctcgggcc agtcggccgg ccagcacgtg gtgacgatca
     7681 cgccgacgtc cgcgcccgcc tcgcgcaggt cctcgcgcag cttggccgtc cactgcgtgc
     7741 tccacttctg cgcgcgcttg gcttcccaga ggatgacgcc ggcctgctgg ccggagcgcg
     7801 tcatgacgcg ctgggacgcg tccgcgcccc gcacgccctt gcggacttcg ctcacgacgt
     7861 cgagcgcgaa ggcggcagcg agttcctcct cgagcacgag ttcgagcacc tcgccctgca
     7921 gctgctgcga gccctgctcc agccggcgct gggtgtcgga gagcttgtcg agcgtgtcgt
     7981 cgagcttctt ctgaagctcg gcctcgcgca ggcgcgcgcg ctccgactcc gccgcgcgga
     8041 ccgtatcggc gatgcgacag cgttcctcgt cgaggcggcg ctgcacgtcc agctcgagct
     8101 ggcgctgctg ctgctcgagc gcctcgcgtt cccggcgcag ctgcagttcg agcgcctgtg
     8161 actgctggag ctgtgcctgc tgggatgcga gccgttcctc gagctcgcgg cgcgtccctg
     8221 ccgcgagttc cacggcgcgg ctcgcggcgc cggcttcgat tgcctcggcc atgctggctt
     8281 cgcgccgggc aatgtcgcgc gcccggtctg ccagcgcgtc gcgttccgcc gcggcctcgc
     8341 gcaactcggc atcccgctgc tccagcgacg cgcgcaggtc ggcttcgcgt cgtgcggcgc
     8401 cttcctgctc cagcgcccgc atctgcgcga gcgactcggc gtgctgccgt cgctgggcgt
     8461 cgagcatctg ctgcagtccc tcgagccgtt cgcgcaacag cgcctcgccc tgggccgcgc
     8521 gttcgcgcgc ccggcgctcc tcgcccgcac gcgcctcgcc ctgcagcttc tcgagcgcgc
     8581 ccacgctcgc gcgctcgagc gcctcgagcg agtggcgcgc aaaaccgtcg gcgaggctga
     8641 actcgtgctc gcagctcggg cacgtgatgg gggtgagggg gccgagaaga agcattggga
     8701 agtggctggt ggttggtggc tagtggctag tggctagtgg ttagtggtta gtggctggtg
//
LOCUS       DASPFR010000064         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_509246, whole genome shotgun sequence.
ACCESSION   DASPFR010000064 DASPFR010000000
VERSION     DASPFR010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509246"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VD737_01560"
     CDS             complement(<1..185)
                     /locus_tag="VD737_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09277.1"
                     /translation="MLHHDVSGMLAAARMDLSRVASRTTDEETLEHVRRVDHLLEQVI
                     RDARREMQRLHPALLDH"
     gene            455..1093
                     /locus_tag="VD737_01565"
     CDS             455..1093
                     /locus_tag="VD737_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXW09278.1"
                     /translation="MRKTSVVVVDDHQVVCAGLRSMINAQPDLGVVGEATSGKEALER
                     LKETQADVMLLDLYLPDMSGLDVLRYARSHYPRTKILVLSSYPEAQFGLNVLRGGASG
                     FIGKGTEAPELMRALRTVARGGRYVGPELAEQLVAGVCGDSNAPLHGKLSEREFQIFC
                     KLAQGESVSQIAARLFLSVKTVSTYRRRILDKMSMKSNAEITSYAIRNDLIR"
     gene            1173..2057
                     /locus_tag="VD737_01570"
     CDS             1173..2057
                     /locus_tag="VD737_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXW09279.1"
                     /translation="MKKQQTRSDSKPRRASKPPQRQRLPGVESKMRPRPASKMREYRA
                     AGRLDGQVALVTGGDSGIGRAVAIAYAKEGADVAIVYLEEHGDARETAAEIEKLGRRC
                     VTLAADLSEVDECHRVVDEAMARLGRLDVLVNNAATQVKQDRFEDISPEQLEQTFRTN
                     VFAMFHVTQRALAHLKDGARIINTASVTAYRGSPHLIDYAATKGAIVAFTRSLGLALV
                     ERGIRVNAVAPGPVWTPLIPASFPPDEVQEFGQNVPLGRAAEPDEIAPCFVFLAADES
                     AFFVGQVLHPNGGEIINT"
     gene            2092..>2608
                     /locus_tag="VD737_01575"
     CDS             2092..>2608
                     /locus_tag="VD737_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09280.1"
                     /translation="MTRGSKDDHAYERPMDEANMGDGSSRTGERDMDDDADGSMQPTM
                     DEGSTGTRGGRGGTGGRRSTGGGGGAGGTGGRSGSGSRGAAGSTAAAARGGRDGRGGG
                     GSRTTTRAKSTGARGKRSTRRKSPAGTRSATRSKTASSRGGSTRAGTGARAKSAGGRS
                     RSTATGRGSARG"
BASE COUNT          442 a          878 c          915 g          373 t
ORIGIN      
        1 aaatgatcga gcagcgccgg gtgcagtcgc tgcatctcgc gtcgggcatc gcgaatcacc
       61 tgctcgagca ggtggtcgac ccggcggacg tgctccagcg tctcctcgtc cgtcgtgcgc
      121 gatgcgaccc gcgacaggtc catccgcgcc gccgcgagca tgccactgac gtcgtgatgc
      181 agcatccgcg acagctccga gcgttcctgt tcgcgctgct gctggaggtg cgcgacaagc
      241 gcggtgcctt ccgacccgat ggcaggggac gatgccgtcc tggctacctt tgctgccatt
      301 ccgtgttgct cttgtggagt gcggttgcgg ggagtatgca gggcacttga acgggccgct
      361 cccgtctgta ggattgttcc gacagcgcga ttgagtcggt gcgtttctgc gtacactggc
      421 atcgtacccc gcatggcgcg gcaggatgca agtcatgagg aaaacttccg tagtagtcgt
      481 ggacgatcac caggtggttt gcgcgggcct gcgatcgatg atcaacgcgc aacccgacct
      541 cggcgtggtc ggggaggcca cttcgggcaa ggaagccctg gaacgcctca aggaaacgca
      601 ggccgacgtg atgctgctcg acctgtacct gcccgacatg tcgggcctcg acgtgctgag
      661 gtatgcgcgt tcgcattacc cgcgcaccaa gatcctcgtg ctgagcagct acccggaggc
      721 gcagttcgga ctcaacgtgc tgcggggcgg agcgagcggc ttcattggca agggcaccga
      781 ggcacccgag ctgatgcgcg cgctgcgcac ggtcgcgcgc ggcggccgct acgtgggccc
      841 ggagctggcc gagcagctgg tcgccggcgt ctgcggcgac tccaacgcgc cgctgcacgg
      901 caagctgtcc gagcgtgagt tccagatctt ctgcaagctg gcccagggcg aatcggtgtc
      961 gcaaatcgcg gcgcgcctgt tcctgagcgt caagaccgtc agcacctacc gtcggcgcat
     1021 cctggacaag atgtcgatga agtcgaacgc cgagatcacg agctacgcga ttcgcaacga
     1081 cctgatccgc tgacgtcgcc tgcgacgcgt ccccgcgtcg gacgaggacc gtgtcacccg
     1141 cgaagtggca cggtccttgc tgtttctgct gcatgaaaaa gcagcagacc cgctctgaca
     1201 gcaaaccacg ccgggccagc aagccaccgc agcgccagcg cctgccgggc gtcgaatcga
     1261 agatgcgtcc acggccggcg tcgaagatgc gcgagtaccg cgcagcgggt cgcctggacg
     1321 gacaggtcgc gctcgtgacc ggcggcgaca gcggcatcgg ccgcgccgtc gcgatcgcgt
     1381 acgccaagga aggtgccgac gtcgcgatcg tctacctcga ggagcacggc gacgccaggg
     1441 agacggccgc cgagatcgag aaactcggcc gccgctgcgt caccctcgcc gccgacctca
     1501 gcgaggtcga cgagtgccat cgcgtggtcg acgaggccat ggcgcgcctg ggccgcctcg
     1561 acgtgctggt caacaacgcg gcaacgcagg tcaagcagga ccggttcgag gacatctcgc
     1621 ccgagcagct cgagcagacc tttcgcacca acgtgttcgc gatgttccac gtcacgcagc
     1681 gcgcgctcgc gcacctgaag gacggcgcgc gcatcatcaa cacggcctcg gtgacggcct
     1741 accgcggcag tccgcacctg atcgactacg ccgctaccaa aggcgcgatc gtagctttca
     1801 cgcgctcgct cggcctggcg ctcgtcgagc gtggcattcg cgtcaacgcc gtcgcgcccg
     1861 gtcccgtctg gacgccactg atccccgcgt ccttcccgcc ggacgaggtg caggagttcg
     1921 gccagaacgt gccgctcggc cgcgcggcgg agcctgacga gatcgcaccg tgcttcgtgt
     1981 tcctcgccgc ggacgaatcc gcgttcttcg tagggcaggt cctgcatccg aacggcgggg
     2041 aaatcatcaa cacttgagcg caggcgaagc ctaatgcaaa ggagtgcact catgactaga
     2101 ggcagcaagg acgatcacgc gtacgagcgg ccgatggacg aggccaacat gggcgacggc
     2161 tccagccgca ccggcgagcg cgacatggac gacgatgccg acggatcgat gcagcccacg
     2221 atggacgagg gctcgaccgg cacgcgcgga gggcgcggtg gcaccggcgg ccgccgcagc
     2281 acgggcgggg gcggcggcgc gggcggcaca ggcggccgca gcggctcggg atcgcgtggt
     2341 gccgcgggca gcaccgccgc tgcggctcgc ggcggacgcg acggtcgtgg tggcggtggc
     2401 agccgcacga cgacgcgtgc caagagcacc ggcgctcgcg gcaagcgctc gacacgccgc
     2461 aagagcccgg cagggacgcg gagtgcgacg cgcagcaaga cggcgtccag tcgtggcggc
     2521 tcgacgcgcg ccggcaccgg cgcgcgtgcc aagtcggccg gcggccggag tcgatcgacg
     2581 gcgaccggac gcggcagcgc acgtggcg
//
LOCUS       DASPFR010000065         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_265477, whole genome shotgun sequence.
ACCESSION   DASPFR010000065 DASPFR010000000
VERSION     DASPFR010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265477"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="VD737_01580"
     CDS             <1..1380
                     /locus_tag="VD737_01580"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HXW09281.1"
                     /translation="LARIASDIDGGAFRTAGDYDELLSRMDGFVAWSGQVVNNLRAGI
                     SKGVVLPKVVVQRTLPQLESFASIEDPRQSVFWRPLLNFPAGLSVDDRRRLLAAFDEK
                     LRTKVLPEYRRLHDYLAQEYLPQARDTVAWSELPGGDFWYAWLVRRQTSTGLTPDQVH
                     ELGLREVARLHAALAGLQPALGIPGETRAVLEAMRTGAASQAVPTPELRTAFAAMHAR
                     VDANLASSFQRVPKAPLEIRVGAAYRAACGATSYYRQASGDGQSPAMLHVDINRGATL
                     PAYQVDAVFLQQAVPGRHLQRSVAHEIPNLPSFRRYGEWNAAYDDGWALYAVSLGSRL
                     GVLTEPSSQLGALAVEMRHAANLVIDSGIHAKGWSRQRAVEYLRANSALGTAEIEAEV
                     DRTIARPAEALAYKVGQLKLFELRREAERQLGPGFDPREFHDQVLGSGSLPLDVLEWK
                     LRRWTAKRR"
     gene            1629..>2216
                     /locus_tag="VD737_01585"
     CDS             1629..>2216
                     /locus_tag="VD737_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09282.1"
                     /translation="MGHFVRRHLRWIAPAALCLASMVGSTQANVFGARPDEDAFDAPR
                     RALDEALPPQARRGTGMLGLLNPGALDATALSFTLPDGRVIHARRQQVVDDRARQLKT
                     WVGTFEEQPGSLAVLGTYRGATTGFLAYGAEVWEILPAPDGRHLVMYRFDDSRLPATE
                     PLVSARKPTAAMSTPLDVRSSEATPEMLQAGYVHDL"
BASE COUNT          353 a          758 c          756 g          349 t
ORIGIN      
        1 ctggcacgga tcgcgtcgga catcgacggc ggcgccttcc gcacggccgg cgattacgac
       61 gaactgctgt cgcgcatgga cggattcgtg gcgtggtcgg gccaggtcgt caacaacctg
      121 cgcgccggca tatccaaagg cgtcgtgctg ccgaaggtcg tcgtgcagcg cacgctgccg
      181 cagctggagt cgtttgccag catcgaggat ccgcgccagt cggtgttctg gcggccgctg
      241 ctcaactttc ccgccgggct ttcggtcgac gatcggcgtc gcctgctggc cgcgttcgac
      301 gagaagctac ggacgaaggt gctcccagag taccggcgcc tccacgatta cctcgcgcag
      361 gagtacctgc cccaggcacg cgacacggtc gcttggtccg agctgcccgg tggtgatttc
      421 tggtacgcgt ggctcgtccg gcgccagacg tcgaccgggc tgacgcccga ccaggtgcac
      481 gaactcggac tgcgcgaggt ggcgcgcctg cacgcggcgc tcgcaggttt gcaaccggcg
      541 ctcggcatac ccggcgagac gcgcgccgtg ctcgaggcga tgcgtaccgg cgccgcgtcg
      601 caggcggtgc ccacgccgga actgcgcacg gcgtttgcag ccatgcacgc ccgcgtcgac
      661 gcgaacctgg cgtcctcgtt ccagcgagtg ccgaaggctc cgctcgagat ccgtgtcggg
      721 gcggcgtatc gagcggcctg cggcgcgacg tcgtactacc gtcaggcgag cggtgatggc
      781 cagtctcctg cgatgctgca cgtcgacatc aatcgcggcg ccacgttgcc ggcctaccag
      841 gtcgacgccg tgttcctgca gcaggcggtg cccgggaggc acctgcagcg gtcggtggcg
      901 cacgagatcc cgaacctgcc gagctttcgc cggtatggcg agtggaacgc cgcatacgac
      961 gacggctggg cactctacgc ggtgtcgctc ggctcgcgcc tcggtgtgtt gaccgaacca
     1021 tcgagccagt tgggcgctct ggccgtggag atgcggcacg cggcaaacct cgtgatcgac
     1081 tctggtatcc atgcgaaagg ctggagccgt cagcgcgccg tcgaatatct gcgcgccaac
     1141 agcgcgctcg gcaccgcgga gatcgaagcc gaagtggatc gaaccattgc ccggcccgcg
     1201 gaggcgctcg cgtataaagt cggtcagctc aaattgttcg agctccgccg cgaggcggaa
     1261 cgccagctcg ggccgggatt cgacccgcgc gagttccacg accaggtgct cggcagcggt
     1321 tccctgccgc tcgacgtgct ggagtggaag cttcgacggt ggacggcgaa gcgacgctga
     1381 gacgccgacg ctcacgaagc ccagcacagc ttgccgattc ctacggcgaa gcgaactgca
     1441 gaaccgcggt ccactccaca aaaaccgccg aaaaacagcg aagcgaacct gtctccgtat
     1501 tgcgacaagc aatcccaaaa cggtgtaatc ccgcctacag cttgtcggca ctcgtgctgc
     1561 actagctgag cagccggggg cgtcgacgcc aggtctctcc cgaactgcga aggacgctcg
     1621 gagaggacat gggtcatttc gttcgccgcc accttcgctg gatcgcaccc gccgcgctgt
     1681 gcctcgcgag catggtgggt tcgacgcagg cgaacgtgtt cggcgcacga ccggacgagg
     1741 acgccttcga cgcgccccgc cgcgcgctgg acgaagcgct gccgccgcag gcccgtcgtg
     1801 gcaccggcat gctcggcctg ctgaatcccg gcgcgctcga cgcgacagcg ttgtcgttca
     1861 cgttgcccga cggccgcgtg atacacgcgc gtcgccagca ggtcgtcgac gatcgtgccc
     1921 ggcagctcaa gacgtgggtt ggcacgttcg aggaacagcc tggcagcctg gcggtgctcg
     1981 gcacctatcg tggcgcgacg accggcttcc tcgcgtacgg cgcggaggtg tgggagatcc
     2041 tgcccgctcc ggacggccgc cacctggtca tgtaccggtt cgacgattcg cggctgcccg
     2101 ccaccgagcc gctggtgtcg gcgcgcaagc ccaccgccgc gatgtcgacg ccgctcgacg
     2161 tgcgctcgag cgaagcgacg cccgagatgc tgcaggccgg ttacgtgcac gacctg
//
LOCUS       DASPFR010000066         6703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_265546, whole genome shotgun sequence.
ACCESSION   DASPFR010000066 DASPFR010000000
VERSION     DASPFR010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265546"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VD737_01590"
     CDS             <1..277
                     /locus_tag="VD737_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09283.1"
                     /translation="LRRLHASWRRHPVLIAAALYGGAPGLPAIFPRWAFADLLALRGE
                     RDPRIVLRRNIERVVRVPMSNAGIDLDTPDDLLHVETALSGSASDVP"
     gene            404..985
                     /locus_tag="VD737_01595"
     CDS             404..985
                     /locus_tag="VD737_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09284.1"
                     /translation="MRGLSRTVSLLGGVSMFLLVGAAQAGPVADQFRSGAFGLPWNAG
                     KSAIQAKYPGGQWDKDAAGTDRYCAPSRQSLLGLPAQHQTRELCFLIGGDGTMASATA
                     RLDATLPSLLAVVNRSRTRFGDFDAVKRDEGAIQSRFTYMMWTKDAPIVVVVGSENDA
                     EGRPSLVAFTVADEATLFTSGAEKVSNRPTGGR"
     gene            complement(1011..1751)
                     /locus_tag="VD737_01600"
     CDS             complement(1011..1751)
                     /locus_tag="VD737_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HXW09285.1"
                     /translation="MQKIVVLNPKGGSGKSTIATNLAAYYAWKGRNVALMDHDPQGSS
                     MRWLKLRPPELPVIHGIAAYERKLGVTRAFALRTPPGTERLVVDTPAAVTSLQMPELT
                     QDAHAIVVPVMPSEIDIHAASRCIADLLLAGRVHRSERRLVIVANRARRYTKAFSSLM
                     RFLDSLRIPVAAVLRDSQAYVRSAECGMGLHEMKGSLLQEDLDTWRPLVEWLEGRTTP
                     ATGFEPARVAVAGEGAERVDACTPTLSC"
     gene            complement(1902..2354)
                     /locus_tag="VD737_01605"
     CDS             complement(1902..2354)
                     /locus_tag="VD737_01605"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HXW09286.1"
                     /translation="MSLKDWISRRLSGLEAESPEDRGRVDLAVAALLVEVLRADYDVA
                     PQERQQVIVSVSRLLGLDATVSAALVAEAERHIDRSHDLYQFTSQLNRSWSEEEKLQL
                     LEALWRVAAADETVHKYEEHLIRRVADLLHVPHSGFIRAKLRATGSGE"
     gene            2559..2834
                     /locus_tag="VD737_01610"
     CDS             2559..2834
                     /locus_tag="VD737_01610"
                     /inference="COORDINATES: protein motif:HMM:NF021559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HXW09287.1"
                     /translation="MSLKTLTIAAAVGGLLAAASASATHHEGMGEAKGDKVKCYGIAK
                     AGKNDCATASHGCAGAAKTDNDPAEWKFAPKAECEQQGGKLEAPSTK"
     gene            2890..3717
                     /locus_tag="VD737_01615"
     CDS             2890..3717
                     /locus_tag="VD737_01615"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HXW09288.1"
                     /translation="MRAGIGLRSPHHEAIEREPPDVGWLEAHTENYFHDGGAAVRTLE
                     RLRARYPLSLHGVGLGLGSADPLDPEHLRRIRAAVHRFEPALVSEHACWTHVDGEHFN
                     DLLPLPGTEEAVEHLARRVRDVQDFLGRQILVENLSAYVGYAGDSLSEGEFLAALVER
                     SGCGLLLDVNNAYVNSVNLGLDLEEYFAALPVHAVQEIHLAGHSRRRVGRHDLLIDDH
                     GSEVCDAVWALYERALRRFGPVPTLIEWDNDVPPLATLVAQAHAADARLDTVKMHAA"
     gene            3707..4507
                     /locus_tag="VD737_01620"
     CDS             3707..4507
                     /locus_tag="VD737_01620"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HXW09289.1"
                     /translation="MRPDVAAPPRLLDAQRALAGALRELDAGTSLSDRFEGPARIAVA
                     DRLAVYRNNARQFFRAALALTYPVLQRRVGEDFFRQLATEYRGAHPSRSGDLHWVGAE
                     FPAWLEIRLHETEYAWLADLARLEWACEEAAAAATEAPVTLQCLAAIPPESVDGVRLR
                     LQPSLRLVASRWPVWSVWSANQVEGAGPVDLTAGAEHCACIGTTDAGVAVFRLGIEAY
                     GLLAELRQDASLADALSRTGTTPDGLAKVLEWAFAHELVVEVSPSAPA"
     gene            complement(4485..4805)
                     /locus_tag="VD737_01625"
     CDS             complement(4485..4805)
                     /locus_tag="VD737_01625"
                     /inference="COORDINATES: protein motif:HMM:NF025614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HXW09290.1"
                     /translation="MKRRSSTVLEHLKRRVSATDAAVIDELYGLEPVYEPDAPARASR
                     FTTEFVSILCPYCWERYDTEVDLSAGSFSHVEDCQVCCQPIQLGCDVDDEGRLVGVKP
                     ERMD"
     gene            complement(4810..>6703)
                     /locus_tag="VD737_01630"
     CDS             complement(4810..>6703)
                     /locus_tag="VD737_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HXW09291.1"
                     /translation="AHGPAPRQAEAAVDGRAATHPRLASEPRVTTVRNWPDVHSRAEP
                     DRFITRHFDLDLEVDFATRRLHGRVTLEVDRVDASARELVLDTRDLAVAAVSTAPPGS
                     DAFTATAYKLDPRHATLGSALRIAMPPSAARVRIEYSTSPDASGLQWLEPEQTAGRRH
                     PFMYSQAQSVHARSFVPLQDTPRVRSTYAASLRVPAGLVAVMAAEPDPANARGAAVFR
                     FRMPQPIPSYLLAVAVGDLEFAATGRRTGVWAEPSVLEAAAWEFGDAETMLEQTEALY
                     GPYRWGRYDVLVLPPSFPYGGMENPRLTFMTPTLLAGDKSLVGVLAHELAHSWSGNLV
                     TNATWRDVWLNEGFTTYFERRIMEAVYGEERARMEWGVGLQDLRIALADLPPGQEWRG
                     AMAPDLSTHLDEDGSAVAYELGALFLYQLEQKYGRAALDAFLAEWFDEHAFTSVTTPQ
                     FVRELREGLIARDPSRIDRGFLRRWIDEPGLPADALLVASDAFARVDAQRQRWESGEL
                     PTAELETSRWTVQEWLQFLNGVQRPHAVGALAELDRRYALTGHRNAEIAHAWYRLALA
                     SGYPGLEAALEGYLTGTGRVRLIRPLYVDLMKTPEGARFARRVYPVARPGYHAIAQRA
                     LDRVVK"
BASE COUNT         1017 a         2361 c         2327 g          998 t
ORIGIN      
        1 cctgcgtaga ctgcacgcca gttggcgcag gcatcccgtg ctcatagcgg ccgccctgta
       61 cggaggtgcg cccggcctgc cggcgatttt tccgcgctgg gccttcgcag acctgcttgc
      121 attgcggggc gagcgcgacc cgcggatcgt attgagacgc aacatcgagc gtgtggtccg
      181 cgttcccatg agcaatgcgg gcatcgacct ggacacgccc gacgacctct tacacgtgga
      241 gaccgcgctc tccggaagcg caagcgacgt tccctaggta ttatgtcaac gctgcgcggc
      301 gcaccttttg agaggcgtgt cgtgctttcg tcgcgagccg gcggcccgcc cggaaaggaa
      361 gcttatgctc atccggcagc tggttttccc tggagtcgat cgcatgcgcg gcctgtcacg
      421 aaccgtttcc ctgctcggcg gtgtctcgat gttcctgctc gtcggtgcgg cccaggcggg
      481 gcccgtcgcg gaccagttcc gcagcggcgc cttcggcctg ccgtggaacg ccggcaagtc
      541 ggcgatccag gcgaagtatc ccggtggcca gtgggacaag gatgccgccg gcaccgaccg
      601 ttactgcgcc ccgagccggc agtcgctgct cggcctgccc gcgcagcacc agacccgcga
      661 actgtgtttc ctgatcggcg gcgacggcac gatggcgagc gccaccgcgc ggctcgacgc
      721 gacgctgccg tcgctgctcg cggtcgtgaa ccgctcgcgc acgcgcttcg gtgacttcga
      781 cgcggtcaag cgcgacgaag gcgcgatcca gtcgcgcttc acgtacatga tgtggaccaa
      841 ggacgcgccg atcgtcgtgg tcgtgggcag cgaaaacgac gcggagggcc gtccgagcct
      901 ggtcgccttc acggtggccg acgaggcgac tctgttcacc tcgggcgccg aaaaggtctc
      961 caaccgtccg accggcggca ggtgatctgc gccttcggcc gggtgaccga ctagcaggac
     1021 agggtcggcg tgcaggcgtc gacgcgctcg gcgccctcgc ctgccacggc cacgcgcgcg
     1081 ggctcgaagc cggtcgccgg cgtcgtgcgt ccctccagcc attcgaccag cggtcgccac
     1141 gtgtccaggt cttcctgcag cagcgagcct ttcatctcgt ggaggcccat accgcactcg
     1201 gccgagcgga cgtacgcttg tgagtcgcgc aggaccgcgg cgaccgggat gcgcaggctg
     1261 tcgagaaacc gcatcagcga actgaacgcc ttggtgtagc gacgggcgcg gttcgccacg
     1321 atcacgagcc gtcgctccga acggtgcacg cgaccggcca gcagcaggtc ggcgatgcag
     1381 cgcgaggcgg cgtggatgtc gatctccgac ggcatgaccg gcacgacgat cgcgtgcgcg
     1441 tcctgcgtca gttccggcat ctgcaacgac gtcacggccg ccggggtgtc gacgaccagc
     1501 cgctcggtgc cgggcggcgt acgcagcgcg aacgcgcgcg ttacgcccag cttgcgctcg
     1561 tatgcggcga tgccgtggat cacgggcagt tccggcgggc gcagcttgag ccagcgcatg
     1621 ctcgacccct gcgggtcgtg gtccatcagt gcgacgttgc gacctttcca cgcgtagtac
     1681 gccgcgagat tggtggcgat ggtcgacttg ccggagcccc ctttcgggtt caggacgacg
     1741 attttctgca ccgtgggcgc ggtcccggga ttttccataa ccgtcgaggt gggaaagctg
     1801 agcggaccgt acccgaccgc accggccacc cgcaagccgg aactgcaatt ctgcggatgg
     1861 ccgacgcggc ggatcgccgc gtcctcatgt cgattagacg tctattcgcc ggagccggtg
     1921 gcgcgcagtt tggcgcggat gaacccggaa tgaggcacgt gcagcaggtc cgcgacccgg
     1981 cgaatcaggt gttcttcgta cttgtgcacc gtctcgtccg cagccgcgac gcgccacagt
     2041 gcctcgagca gctgcagttt ttcttcttcc gaccaggatc ggttgagttg cgaggtgaac
     2101 tggtacaggt cgtgcgacct gtcgatgtgc cgttcggcct cggccaccag cgcggcgctc
     2161 accgtggcgt cgagccccag cagtcgtgac accgagacga tgacctgctg ccgctcctgc
     2221 ggggcgacgt catagtcggc gcgcagcacc tcgaccagca gggccgcgac cgcgaggtcc
     2281 acgcgaccgc ggtcctcggg actttcggcc tcgagaccgc tcaatcttcg gctgatccag
     2341 tccttgaggc tcatcgcagg cgccgcacgg tgcagggggc gaatgctgcc acccgcaccg
     2401 cccgattcca accgggtcgc gtcggccgga cacccgacgc ataatccggt cggcgatccg
     2461 gcgcgaactg cgccatgccg ttccacgtgg ggttcgcccc gagcgcggac cccggatcgg
     2521 ccccaccctc cagtcagaca cgcagaggag acccaacgat gagcctcaag actctaacga
     2581 tcgccgccgc cgtcggcggc ctgctcgccg ccgcctccgc ctcggccacc catcacgagg
     2641 gcatgggcga agccaagggc gacaaggtca agtgctacgg catcgccaag gccggcaaga
     2701 acgattgcgc gactgcgagc cacggctgcg ccggggccgc caagacggac aacgacccgg
     2761 ccgaatggaa gtttgcgccg aaggccgagt gcgaacagca gggcggcaag ctcgaggcgc
     2821 cgtcgaccaa gtgaggcgac catgaccggc cgaacgccgc gcgcgtcgac cgccggaagt
     2881 ccggtcccgg tgcgagccgg gatcgggctg cgctcccccc atcacgaggc gatcgagcgg
     2941 gagccccccg acgtcggctg gctcgaggcg cacaccgaaa actatttcca cgatggcggc
     3001 gcggcagtcc gcacgctcga acgcctgcgc gcgcgctacc cgctgagcct gcacggcgtc
     3061 ggactcgggc tcggctcggc cgacccgctc gatcccgagc acctgcgtcg catccgcgca
     3121 gccgtgcacc ggttcgaacc cgcgctcgtg agcgagcacg cctgctggac gcacgtcgac
     3181 ggcgagcact tcaacgacct gctgccgctg ccggggaccg aggaagccgt cgagcacctg
     3241 gcccgccgcg tgcgtgacgt gcaggacttc ctcggtcggc agatcctggt cgagaacctg
     3301 agcgcgtacg tgggttacgc cggggactcg ctgtcggaag gcgagttcct cgccgcgctg
     3361 gtcgagcgct cgggctgcgg cctgctgctc gacgtcaaca acgcatacgt caactcggtg
     3421 aacctcgggc tggacctcga agagtacttc gccgccctgc cggtgcacgc ggtgcaggaa
     3481 atccacctcg ccggccattc gcgccgacgc gtcggccggc acgacctgct gatcgacgac
     3541 cacgggtccg aagtgtgcga cgccgtgtgg gcgctgtacg agcgcgcgct gcgccgcttc
     3601 ggcccggtgc cgacgctgat cgagtgggac aacgacgtgc cgccgctggc cacgctcgta
     3661 gcgcaggccc acgcggccga cgcgcgcctc gacacggtga agatgcatgc ggcctgacgt
     3721 ggcggcgcca ccacgcctgc ttgatgcgca gcgtgccctc gcaggtgcac tgcgcgaact
     3781 ggacgccggg acgtcgctga gcgatcgatt cgaagggccg gcgcggatcg ccgtcgccga
     3841 tcgactcgcc gtctaccgca acaacgcccg gcagttcttc cgcgcggcgc tcgcgctcac
     3901 gtacccggtg ctgcaacgca gggtcggcga ggacttcttc cggcagctcg cgaccgaata
     3961 ccgcggggcg cacccttccc gcagcggcga cctgcactgg gtcggagcgg agtttcccgc
     4021 ctggctggag atccggctgc acgagacgga atacgcgtgg ctggccgacc tcgcgcggct
     4081 cgagtgggcg tgcgaggaag cggctgcggc cgcgaccgag gcgccggtca cgctgcagtg
     4141 cctggccgcg atcccgccgg aaagcgtcga cggcgtgcgc ctgcggctgc agccgtcgct
     4201 gcggctggtg gcctcgcgct ggccggtctg gtccgtctgg agcgcgaacc aggtcgaggg
     4261 cgccgggccg gtcgatctca ccgcgggcgc cgaacactgc gcgtgcatcg ggacgaccga
     4321 tgcgggcgtc gcggtgttcc gtctcgggat cgaggcatac gggctgctcg ccgagctgcg
     4381 ccaggacgcg agcctcgccg acgcgctgtc acgaacggga accacgccgg acgggctcgc
     4441 gaaagtgctc gaatgggcgt ttgcccacga gctcgtcgtc gaggtcagtc catccgctcc
     4501 ggcttgacgc cgaccaggcg accttcgtca tcgacgtcac aaccgagctg tatcggctgg
     4561 cagcagacct ggcagtcctc gacgtggctg aacgacccgg cagacaggtc gacttcggtg
     4621 tcgtaccgct cccagcagta tggacacagg atcgaaacga actcagtcgt gaacctcgac
     4681 gcgcgcgccg gggcgtcggg ctcgtacacg ggctcgagac cgtagagttc gtcgatcacc
     4741 gcggcgtccg tcgcggagac gcgccgcttc aggtgttcca ggacggtgga gctccgccgt
     4801 ttcatcgcct cactttacca cccggtcgag cgcgcgctgc gcaatcgcgt gatagcccgg
     4861 acgcgccacg ggatagacgc ggcgcgcgaa tcgcgcgcct tccggagtct tcatcaggtc
     4921 cacgtagagc ggccggatca gccgcacccg gccggtgccc gtcaggtagc cttccagtgc
     4981 cgcctccagc ccgggataac cgctggccag cgcgagccga taccaggcgt gcgcgatctc
     5041 ggcgttgcgg tggcccgtca gggcatagcg ccggtcgagc tccgcgagcg cgccgacggc
     5101 gtgcggccgc tgcacgccat tcaggaactg cagccattcc tgcacggtcc agcgcgaggt
     5161 ctcgagctcg gccgtgggca gctcgccgga ctcccagcgc tggcgctgcg cgtcgacgcg
     5221 ggcgaacgca tcggaggcga cgagcaacgc gtcagcgggc aagccgggtt cgtcgatcca
     5281 gcggcggagg aacccgcggt cgatccgcga ggggtcccgt gcaatcaacc cctcgcgcag
     5341 ctcgcgcacg aactgcggcg tcgtgacgct cgtgaacgcg tgctcgtcga accactccgc
     5401 gaggaaggcg tcgagcgccg cgcggccgta cttctgctcg agctggtaca ggaacaacgc
     5461 ccccagttcg taggccacgg ccgagccgtc ctcgtccagg tgcgtactga ggtccggcgc
     5521 catcgccccg cgccactcct ggccgggcgg gaggtccgcc agcgcgatgc gcaggtcctg
     5581 caggccgacg ccccactcca tccgcgcgcg ctcttcgccg tagacggctt ccatgatccg
     5641 tcgttcgaaa tacgtcgtga agccctcgtt cagccagacg tcgcgccagg tggcattggt
     5701 gacgaggttg cccgaccacg agtgcgcgag ctcgtgcgcg agtacgccca ccagcgactt
     5761 gtcgccggcg agcagcgtcg gcgtcatgaa cgtgagccgc gggttctcca tgccgccgta
     5821 cggaaaggac ggtggcagca cgagcacgtc gtaacggccc catcggtagg ggccgtacag
     5881 cgcctcggtc tgctcgagca tcgtctcggc gtccccgaac tcccacgcgg ccgcctcgag
     5941 caccgacggc tccgcccaca cgcccgtgcg cctgcccgtc gccgcgaact cgaggtcgcc
     6001 gaccgcgacg gcgagcaggt acgacgggat cggctgcggc atccggaagc ggaacaccgc
     6061 ggctccccgg gcgttcgcag gatccggctc cgcggccatg acggcgacga ggcccgccgg
     6121 cacgcgcagc gaagcagcat aggtcgaccg cacgcggggc gtgtcctgca gcggcacgaa
     6181 cgagcgtgcg tgcaccgact gtgcctggct gtacatgaac ggatggcggc gacccgcggt
     6241 ctgctcgggc tcgagccact gcaggccgga agcgtccggt gacgtcgagt actcgatgcg
     6301 cacgcgcgca gccgacggcg gcatggcgat gcgcagggcg ctgccgagcg tcgcgtgtcg
     6361 cggatcgagc ttgtaggccg tggccgtgaa cgcgtcgctg cccggaggcg ccgtcgagac
     6421 cgctgccacg gccaggtcgc gggtgtcgag cacgagttcc cgcgcggacg cgtcgaccct
     6481 gtcgacctcg agcgtgacgc ggccgtggag gcgtcgcgtc gcgaaatcca cttccaggtc
     6541 gaggtcgaaa tggcgcgtga tgaatcggtc gggttcggcg cggctgtgca cgtccggcca
     6601 gttgcgcacg gtcgtcaccc gcggctccga cgccagccga gggtgggtcg cggcgcggcc
     6661 atcgaccgcc gcctccgcct gcctcggcgc tggtccatgg gcc
//
LOCUS       DASPFR010000067         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_997568, whole genome shotgun sequence.
ACCESSION   DASPFR010000067 DASPFR010000000
VERSION     DASPFR010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997568"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VD737_01635"
     CDS             complement(<1..613)
                     /locus_tag="VD737_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007150215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXW09292.1"
                     /translation="MTELDRFRAEVRDWIAANYPPSMQSPLGLESPDTPWGGRKTRYA
                     NPDVTSWMERMAAKGWITPEWQPEHGGAGLSPGHARVLREELAAARCRPAAISFGLMM
                     LAPVLHEFASEEQRRRFLPPIARGEIRWCQGYSEPSAGSDLAGLKTRAEDLGDHWLVN
                     GQKIWTSYADQADWIFCLVRTNAESKHGGISFLLIDMSTPGIEV"
     gene            complement(683..1882)
                     /locus_tag="VD737_01640"
     CDS             complement(683..1882)
                     /locus_tag="VD737_01640"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXW09293.1"
                     /translation="MLPLLSGIRILDFTTIVLGPYATRTLGDLGADVVKVEPLQGDLF
                     RTVRPGRSRGMGAGFLGFNRNKRSLALDLKHPAAREVLDRLVRGADVVVHNMRPKSAV
                     PLGLDYARLAALRPGLIYAFAAGYDQRGPNAAVPAYDDVIQAASGVAALNANERGEPR
                     YLPTILCDKVGGLHLAIAVAAAIARRARTGEGGCIEAPMFESVVSFLMSEQLGGATFE
                     PPLGPTGYDRLSAPNRKPYRTRDGHVAILPYTTTHWTAFFELIGQPELAHSPRVQDPV
                     VRSQSADALYALIAEAAPSRTSAEWLEALHARDIPCARVNAIGELLGDPHLQATGFFR
                     EYEHPTEGTLRDARTPFRLHDGQEDDDRPAPRLGAQSRAVLRECGYDDAAIDRLIEIR
                     AVACAQE"
     gene            2025..>2290
                     /locus_tag="VD737_01645"
     CDS             2025..>2290
                     /locus_tag="VD737_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXW09294.1"
                     /translation="MGPLHGVRVIELAGIGPAPFCAMMLADMGAEVIRIDRAAAAPGT
                     READCLLRNRRSLALDLKHPEAIATVLRLVERADALIEGFRPGV"
BASE COUNT          323 a          816 c          798 g          353 t
ORIGIN      
        1 gtacctcgat gccgggcgtc gacatgtcga tcagcaggaa cgagatgccg ccgtgcttcg
       61 actccgcatt ggtgcgcacc aggcagaaga tccagtcggc ctggtccgcg tacgaggtcc
      121 agatcttctg gccgttgacg agccagtggt cgccgaggtc ctcggcccgg gtcttgagcc
      181 cggccaggtc cgaaccggcg ctcggctccg agtagccctg gcaccagcgg atctcgccgc
      241 gtgcgatcgg cggcaggaag cgccgacgct gctcctcgct ggcgaactcg tgcagcaccg
      301 gcgcaagcat catcagcccg aacgagatcg cggccggccg gcagcgcgcg gcggcgagtt
      361 cctcgcgcag cacccgtgcg tgccccggcg acagccccgc gcccccgtgc tcgggctgcc
      421 actcgggcgt gatccagccc ttcgcggcca tgcgctccat ccacgacgtg acgtccgggt
      481 tggcgtagcg cgtcttgcga ccgccccacg gcgtgtcggg gctctccagg ccgagcggcg
      541 attgcatcga cggcggatag ttggccgcga tccagtcgcg gacctcggca cggaatcggt
      601 cgagttcggt catcggcact ccatcgtcat cgtccactcc atcgctgact cctaggcgct
      661 ccgtcggatc agcgatcact tctcactcct gggcgcacgc cacggcgcgg atctcgatca
      721 ggcggtcgat cgcggcgtcg tcgtagccgc attcgcgcag cactgcgcgg ctctgcgcgc
      781 caaggcgcgg cgcgggccgg tcgtcgtctt cctggccatc gtggagccgg aacggcgtcc
      841 gcgcgtcgcg cagcgtgcct tcggtcgggt gctcgtattc gcggaagaag cccgtggcct
      901 gcaggtgcgg atcgccgagc aactcgccga tcgcgttcac gcgcgcgcac gggatgtcac
      961 gcgcgtgcag cgcctccagc cactccgcgc tcgtgcgcga cggcgccgcc tccgcgatca
     1021 gcgcgtagag cgcgtcggca ctctggctgc gcacgacggg atcctgcacg cggggcgaat
     1081 gtgcgagctc cggctgtccg atcagttcga agaacgcggt ccagtgcgtc gtcgtgtacg
     1141 gcaggatcgc aacatgaccg tcgcgcgtgc ggtacggctt gcgattcggg gcgctcagcc
     1201 ggtcgtagcc ggtcggcccg aggggcggct cgaacgtggc gccgcccagc tgttccgaca
     1261 tcaggaaaga caccacgctc tcgaacatcg gcgcttcgat acagccgcct tcgccagtgc
     1321 gcgcgcgccg tgcgatcgcc gcggccaccg cgatcgcgag gtgcagcccc ccgaccttgt
     1381 cgcacaggat ggtcggcagg taacgaggtt cgccgcgctc gttggcgttc agcgccgcga
     1441 cgcccgaggc ggcctggatc acgtcgtcgt acgccggcac cgccgcgttc ggtccgcgct
     1501 ggtcgtagcc ggccgcgaac gcgtagatca ggcccggacg cagcgcggcc agccgcgcgt
     1561 aatcgagccc gagtggcacg gctgacttcg gccgcatgtt gtgcacgacg acgtcggcgc
     1621 cgcgcacgag ccggtcgagc acctcgcgcg cggccgggtg cttcaggtcg agcgcgagcg
     1681 accgcttgtt gcgattgaag ccgaggaacc ccgcgcccat gccgcgcgaa cgcccggggc
     1741 ggaccgtccg gaacaggtcg ccctgcagcg gctcgacctt gacgacgtcc gcgcccaggt
     1801 cgccgagcgt acgcgtcgcg tacggcccga gcacgatcgt cgtgaaatcc aggatgcgaa
     1861 tcccactcag cagcggcagc atgcgtcgtc gatacctgcg cacgcagccc gcgcgccgaa
     1921 gttcccattg accggcaagt gtatcgaagc aatagtgccc gcgggtgcgc gtccgcgtgc
     1981 ctgccttcgg atttgcgtcg cacgtgagtg gggtgaacgc gcgcatgggc ccgttgcacg
     2041 gcgtcagggt gatcgagctc gcgggcatcg gcccggcgcc gttctgcgcg atgatgctcg
     2101 ccgacatggg cgccgaggtg atccgcatcg accgcgcggc cgcagcccca ggcacgcgcg
     2161 aggccgactg cctgctgcgc aatcgtcgct cgctcgctct ggacctcaag cacccggagg
     2221 cgatcgcgac ggtgctcagg ctggtcgagc gtgccgacgc gttgatcgag ggcttccgtc
     2281 cgggcgtgat
//
LOCUS       DASPFR010000068         7413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_875928, whole genome shotgun sequence.
ACCESSION   DASPFR010000068 DASPFR010000000
VERSION     DASPFR010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875928"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..696)
                     /gene="pgl"
                     /locus_tag="VD737_01650"
     CDS             complement(1..696)
                     /gene="pgl"
                     /locus_tag="VD737_01650"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HXW09295.1"
                     /translation="MRVIIAPDAEAAAHGAAGELARACAAAVAERGRAVIALSGGRSP
                     LRAFELFARAPLPWASMVVAQVDERCVPAQDARRNLAPLQRLLVEQGELPRDNLLGMP
                     VERGDLERAADDYAAQLRERLGPDGEFDIVQLGLGDDGHTASLVPGDPVLDVDERDVA
                     VTARPYQGTRRMTLTYRRLQSARERLWFVTGAGKAATLAELIEGTGSSPAVRVRREDT
                     VVVTDRAASPLSR"
     gene            complement(687..2093)
                     /gene="zwf"
                     /locus_tag="VD737_01655"
     CDS             complement(687..2093)
                     /gene="zwf"
                     /locus_tag="VD737_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HXW09296.1"
                     /translation="MNDDYSDALVVFGITGDLAFKKILPALENLERRGRLPPTVVGVA
                     RGGLTTAHLVERLRASLRAHGDGTDSAALQRLEAKLRYVDGDYQDDATFAALRTALGT
                     AQRPCHYLAIPPSLFATVAEKLGVSGSAKGARVVVEKPLGRDLASARTINRALQRVFA
                     EHSIFRIDHFLGKEPVQNLLYFRFANSFVEPIWNRTHVEHVQITMAERFGVEGRGRLY
                     EELGAVRDVVQNHLLQVLAILAMEPPVGIGSEAMRDEKMKVLRAVRAPERRDLVRGQY
                     GGYRGEPGVAPDSDVETYAAMRFEIDSWRWAGVPFFVRTGKRLPVTATEVLATFRRPP
                     QRLFDEPLPPRMNYLRFRLGPDRVAIALGARIKAHGESMVGHETELFACNEQADEMLP
                     YERLLGDAMRGDAGLFARQDSVELAWDIVDRLLASGPQAEPYAPATWGPPAASRMVER
                     FGGWYDPPADPVSSPPCG"
     gene            2302..4599
                     /locus_tag="VD737_01660"
     CDS             2302..4599
                     /locus_tag="VD737_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HXW09297.1"
                     /translation="MTETRHLWKILGAIMALSFGILLFLGREIYLTAPPVPSAVKTTT
                     GQTLFTGADIQTGREVWQTIGGHQLGSIWGHGSYVAPDWSADWLHREATGLLELWAAR
                     EHGKSFDALTAEQRGGLEARLKEEIRANTYDAATGEVIVSEDRAQVMAQVADHYQDLF
                     GSDLELNTLRSQYAMKVNTVPDPARREALTAFFFWTSWSTATNRPDDDVTYTSNWPSE
                     PLIGNRPTAPTFMWTFVSIFIMLGGIGALAWYHASVQGREPAPVVPDSDPLKSLKPTA
                     SMKATAKYFWVVTALILLQVVLGGITAHYAVEGQSFYGIPLSEILPYSVTRSWHLQLA
                     VLWIATAWLATGLYIAPAISGHEPKLQRLGVNFLFTCLLVIVVGALGGQWLAVMQKMG
                     LAANFWFGHQGYEYTDIGRFWQIFLFVGLMLWLVLVGRALWPALKRRDDARSITMLLF
                     LSTVAIGLFYGAGLMWGRTTHLAMVEYWRWWVVHLWVEGFFEVFATAVIAFLFTKLGL
                     VRGRSATSAVLFATVIFLSGGVLGTLHHLYWTGTPTSVLAFGAAFSALEVVPLVLIGF
                     EAYENYKHSKAKPWIQAYRWPILFFVSVAFWNLVGAGLFGFLINPPLALYYMQGLNTT
                     PLHGHTALFGVYGMLGIGLMLFCLRGLSLTGFWDEKLLKGTFWTLNGGLAAMALLSLA
                     PVGLLQVHAAVTEGFWYARSAEFLQQPLMQTLVWLRMPGDVIFSVGVCLLALFVFRLF
                     VGKRKDTVAVGTPGTVEQAATGSGR"
     gene            4715..5950
                     /locus_tag="VD737_01665"
     CDS             4715..5950
                     /locus_tag="VD737_01665"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HXW09298.1"
                     /translation="MNTPAPSPAGRPLVAAPHRTLFFAGLLSLLVATAWWGLHVIARY
                     YGTPAVALVLQPAPIWAHAWLMLFAVFPTIFFGFLFTVFPRWMNGPEVARAEYVATAL
                     LFVTGTVLWLVGTLAGPPWLLLGCLCTGLALTVALYALFRVLLAAPKVVSHAVVVLVA
                     LSVQLVALAGYAWGLAQANDFALHFAIRASLWGGLLPVFFAVCHRMIPFFSQGVVPGY
                     VPWRPLWVLVAVVALAYARLLLGTAGALAVLPLVDVALFALTALCAVRWTSFRSRGNP
                     LLWTLYAGYAWLPVAMLLQTARDGSFTLTGEWALGRAPIHALGMGFFGGMLVAMVTRV
                     TMGHSGRPLRMDRSTLACFLLLQAGALLRVAGEVIAAPAAVQWFLLGSLALWLGALAP
                     WIVRLAGIYLAPRVDGRPG"
     gene            5950..6345
                     /locus_tag="VD737_01670"
     CDS             5950..6345
                     /locus_tag="VD737_01670"
                     /inference="COORDINATES: protein motif:HMM:NF016161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HXW09299.1"
                     /translation="MAAWYLQLRHAHIAFAILSVAIFTVRGLLMLTNSRHVNSPWLKY
                     PSYMVDTLLLTAALMLTTVIHQYPFQAGWLTMKVVLLVVYVVLGSIALKRGRTPTIRV
                     VALVSAWLTVGFLFTVARAHHPLGIFAMS"
     gene            6445..7143
                     /locus_tag="VD737_01675"
     CDS             6445..7143
                     /locus_tag="VD737_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXW09300.1"
                     /translation="MTVHDPNVLAGIRTHHLFAGLAPPQLQRLLATAHVEDAEAGQVL
                     FDRGQPARHFYIVIDGQVNLALYSKTGEEKIVDILGPGQSFAEAVMFMSGPVYPVSAV
                     AAAPSRIARFSSTDYVAMLRESPETCLRMLGHLSQRLHMRIREIEYLTLESATHRLVR
                     LIEARIPAGAEGPVEVQLQESRQEIASRLSMKPETLSRILRHLSDNGAIEVHGRNLVV
                     PDPTRLASILESAS"
BASE COUNT          998 a         2639 c         2538 g         1238 t
ORIGIN      
        1 tcagcgggag agcgggctcg cggcgcgatc cgtcacgacg acggtgtctt cgcgacgcac
       61 gcgcacggcc ggtgaagacc cagtgccttc gatcagctcg gcgagcgtcg cggccttgcc
      121 cgcgcccgtg acgaaccata ggcgctcgcg cgccgactgc agccgtcgat acgtcagcgt
      181 catccgtcgc gtgccctggt acggccgcgc ggtgaccgcg acgtcgcgct cgtcgacgtc
      241 gagcaccggg tcgcccggca cgagcgacgc ggtgtgcccg tcgtcgccca gtccgagctg
      301 cacgatgtcg aactcgccgt cgggaccgag ccgctcgcgc agctgcgcag cgtaatcgtc
      361 cgcggcgcgt tcgaggtcgc cgcgctcgac gggcatcccg agcaggttgt cgcgcggcag
      421 ctcgccctgc tcgaccagca gtcgctgcag cggcgccagg ttccgtcgcg cgtcctgcgc
      481 gggcacgcat cgctcgtcga cctgcgccac gaccatcgat gcccagggca gcggcgcgcg
      541 cgcgaacagt tcgaacgcgc gcagtggcga acgaccgccg ctcagtgcga tgaccgcgcg
      601 tccccgctcg gcgaccgccg ccgcgcacgc gcgggcgagt tcgccggccg cgccgtgggc
      661 cgcggcctcg gcgtccggcg cgatgatcac ccgcatggcg gggatgacac ggggtcggcc
      721 ggcgggtcgt accagccgcc gaaacgctcg accatgcgtg aggccgccgg ggggccccag
      781 gtggccggcg cgtacggctc cgcctggggg ccgctggcga gcagccggtc gacgatgtcc
      841 caggcaagct ccacggagtc ctgccgcgcg aagaggcccg cgtcgccgcg catcgcgtcg
      901 ccgagcagcc gctcgtaggg cagcatctcg tcggcctgct cgttgcaggc gaagagctcg
      961 gtttcgtgcc cgaccataga ctcgccgtgc gctttgatcc gcgcgccgag cgcgatcgcg
     1021 acgcggtcgg gaccgagccg gaagcgcagg tagttcatac gtggcggcag cggctcgtcg
     1081 aacagccgct gtggcggacg acggaacgtc gcgagcacct ccgtcgcggt caccggcagc
     1141 cgcttgcccg tgcgcacgaa gaacggcacg ccagcccagc gccacgagtc gatctcgaag
     1201 cgcatcgccg cgtacgtctc gacgtcggaa tccggcgcga cgccgggctc gccgcgatag
     1261 ccgccgtact gcccgcgcac caggtcgcga cgctcgggcg cccgcaccgc gcgcaacacc
     1321 ttcatcttct cgtcacgcat cgcctccgag ccgatgccga ccggcggctc catggccagg
     1381 atcgcgagca cctgcagcag gtggttctgc acgacgtcgc gcactgcacc gagctcctcg
     1441 tacagccggc cacggccctc cacgccgaag cgctctgcca tcgtgatctg cacgtgctcg
     1501 acgtgcgtgc ggttccagat cggctcgacg aacgaattgg cgaaccggaa gtacagcagg
     1561 ttctgcacgg gttccttgcc gaggaagtgg tcgatccgga agatcgagtg ctcggcgaag
     1621 acgcgctgca gcgcccgatt gatcgtccgc gcgctcgcca ggtcacgccc gagcggtttc
     1681 tcgacgacga cccgcgcgcc cttcgcggat ccggacacgc cgagcttctc cgcgacggtc
     1741 gcgaacaggc tgggcgggat cgccaggtaa tggcacgggc gctgcgcggt gccgagcgcc
     1801 gtccggagcg ccgcgaaagt ggcgtcgtcc tggtagtccc cgtccacgta ccgcagcttc
     1861 gcttcgagtc gctgcaacgc ggccgagtcc gtgccgtcgc cgtgcgcgcg caggctggcg
     1921 cgcagccgct cgacgaggtg cgcggtggtg agcccgccgc gcgccacgcc gacgaccgtc
     1981 ggcggcaaac gcccgcgccg ctcgaggttc tcgagcgccg gcaggatctt cttgaacgcc
     2041 aggtcgccgg tgatgccgaa gacgaccagc gcgtcggagt aatcgtcgtt catgcccgcg
     2101 tccctgcccc gtgccgcgcc gtcactcacg cgagcatatc gacgacgccg cgcatcggca
     2161 cgtcgagatg cctatcccgt tgcggagagt acctagggcg cggccgggcg caattgatct
     2221 cggtcaaggc ggccgcggcc ggccggcgcc acgattcgtg ccatcccgcg gcgaaccgcg
     2281 ggcacgccac ggagttacca catgaccgaa acacgacacc tgtggaagat cctgggagcg
     2341 atcatggcgc tctcgttcgg gatcctcctg ttcctgggac gtgagatcta cctcacggcc
     2401 ccgcccgtcc cgtccgccgt gaagaccacg acggggcaga ccctgttcac cggcgccgac
     2461 atccagaccg gccgcgaagt ctggcagacg atcggcggac accagctcgg ctcgatctgg
     2521 ggccacggca gctacgtcgc acccgactgg tcggccgact ggctgcatcg cgaagccacc
     2581 gggctgctcg agctctgggc cgcgcgcgag cacggcaagt cgttcgacgc gctcaccgcc
     2641 gagcagcgcg gcggcctcga ggcacgcctg aaggaggaaa tccgcgcgaa cacctacgac
     2701 gccgccaccg gcgaggtgat cgtgtccgag gatcgcgccc aggtgatggc gcaggttgcc
     2761 gaccactacc aggacctctt cggctccgac ctcgagctca acacgctgcg ctcgcagtac
     2821 gcgatgaagg tgaacacggt gcccgacccg gcgcgccgtg aagcgctcac cgcgttcttc
     2881 ttctggacgt cgtggtcgac ggcgacgaac cgcccggacg acgacgtgac ctacacgagc
     2941 aactggccct ccgagccctt gattggcaac aggccgaccg cgcccacctt catgtggacg
     3001 ttcgtcagca tcttcatcat gctcggcggc atcggcgcgc tcgcctggta tcacgcgtcg
     3061 gtgcagggca gggagccggc acccgtcgtg ccggacagcg acccgctcaa gtcgctcaag
     3121 cctacggcgt cgatgaaggc gacggccaag tacttctggg tcgtcacggc gctgatcctg
     3181 ctgcaggtcg tcctgggcgg catcacggcg cactacgcgg tcgagggcca gtcgttctac
     3241 ggcatcccgc tgtccgagat cctgccgtac tcggtgacgc gcagctggca cctgcagctc
     3301 gcggtgttgt ggatcgcgac cgcctggctc gcgaccggcc tgtacatcgc gccggcgatc
     3361 tccgggcacg aaccgaagct gcagcgcctc ggcgtcaact tcctgttcac ctgcctgctc
     3421 gtgatcgtcg tgggtgcgct cggcggacag tggctcgccg tgatgcagaa gatgggcctg
     3481 gcggccaact tctggttcgg ccaccagggc tacgagtaca ccgacattgg tcgcttctgg
     3541 cagatcttcc tgttcgtcgg cctgatgctg tggctcgtgc tggtcggtcg cgcgctgtgg
     3601 cccgcgctca agcgccgtga cgacgcgcgc tcgatcacga tgctgctgtt cctctcgacc
     3661 gtcgcgatcg gactcttcta cggcgccggc ctgatgtggg ggcgcaccac gcacctcgcg
     3721 atggtcgaat actggcgctg gtgggtcgtc cacctgtggg tcgaaggctt cttcgaggtc
     3781 ttcgcgacgg ccgtcatcgc cttcctgttc accaagctcg gcctggtccg tggcaggtcg
     3841 gcgaccagcg cggtgctgtt cgcgacggtg atcttcctga gcggtggcgt gctcggcacg
     3901 ctgcatcacc tctactggac cggcacgccg accagcgtgc tcgcgttcgg tgccgcgttc
     3961 tcggcgctcg aagtcgtgcc gctcgtgctg atcgggttcg aggcgtacga gaactacaag
     4021 cacagcaagg cgaagccgtg gatccaggcg taccgctggc cgatcctgtt cttcgtgtcc
     4081 gtcgcgttct ggaacctggt cggcgccgga ctgttcggct tcctgatcaa tccgccgctc
     4141 gcgctgtact acatgcaggg cctcaacacg actccgctgc acggccacac tgcgctgttc
     4201 ggcgtgtacg gcatgctcgg catcggcctg atgctgttct gcctgcgcgg cctgagcctc
     4261 acggggttct gggacgagaa gctgctcaag ggcacgttct ggacgctgaa cggcggcctg
     4321 gcggccatgg cgctgctctc gctcgccccg gtcggcctgc tgcaggtgca cgcggcggtg
     4381 acggaaggct tctggtatgc gcgctcggcc gagttcctgc agcagccgct gatgcagacg
     4441 ctcgtctggc tgcgcatgcc gggcgacgtg atcttcagcg tgggcgtgtg cctgctcgcg
     4501 ctgttcgtct tccggctgtt cgtcggcaag cgcaaggaca ccgtggcggt ggggaccccc
     4561 gggaccgtcg aacaggcggc caccggttcg ggacgctaga gtccttggtc cttgggcagc
     4621 gggcgctcgc aagggcgccc gctgtttttt tgtggaggcg tcgcttgata ccctcgccgg
     4681 ctcgcccgct cgacctcgtc gaaacatcgc agacatgaac acacccgctc cctcgcccgc
     4741 cggccggccg ctcgtcgcgg caccgcaccg caccctgttc ttcgcaggcc tgctgagcct
     4801 gctggttgcc accgcgtggt ggggactgca cgtcatcgct cgctactacg gcacgccggc
     4861 cgtcgccctc gtgctgcagc cggccccgat ctgggcccac gcctggctga tgctgttcgc
     4921 ggtgttcccg acgatcttct tcggtttcct gttcaccgtg ttcccacggt ggatgaacgg
     4981 gcccgaggtg gcccgcgccg agtacgtcgc cacggccctg ctgttcgtca cgggcacggt
     5041 gctgtggctc gtgggcacgc tcgcgggtcc gccgtggctg ctgctcgggt gcctctgcac
     5101 cggtctcgcg ctcaccgtcg cactgtacgc gctgtttcgc gtgctgctcg cggcccccaa
     5161 ggtcgtctcg cacgcggtcg tcgtgctcgt ggcgctcagc gtgcagctgg tcgcgctcgc
     5221 gggctacgcg tgggggctcg cgcaggccaa cgacttcgcg ttgcacttcg cgatccgcgc
     5281 gtcgctctgg ggcgggctgc tgccggtgtt cttcgcggtg tgccaccgca tgatcccgtt
     5341 cttctcgcag ggcgtcgtgc ccgggtacgt gccgtggcgt ccgctgtggg tgctggtcgc
     5401 ggtcgttgcg ctcgcgtatg cccgcctgct gctcgggacg gccggtgccc tcgcggtgct
     5461 gcccctggtg gacgtcgcgc tgttcgcact gacggcgctc tgcgccgtgc gctggacgtc
     5521 gtttcgctcg cgcggcaacc cgctgctgtg gaccctgtat gccggctacg catggctgcc
     5581 ggtcgcgatg ctgctgcaga ccgcgcgtga cggcagcttc acgctgacgg gcgagtgggc
     5641 gctcgggcgc gcgccgatcc acgcgctcgg catgggattc ttcggtggca tgctcgtcgc
     5701 catggtcacg cgcgtgacga tgggccactc cggacggccg ctgcgcatgg atcgctccac
     5761 gctcgcctgc ttcctgctgc tgcaggccgg cgcgctgctg cgcgtggccg gcgaagtgat
     5821 cgcggcgccg gcggccgtgc agtggttcct gctcggcagc ctcgcactct ggctcggcgc
     5881 gctcgccccg tggatcgttc gcctcgcggg catctacctg gcaccgcgcg tggacgggag
     5941 gccgggctga tggccgcgtg gtacctgcag ctgcggcacg cgcacatcgc gttcgcgatc
     6001 ctttccgtgg cgatcttcac cgtgcgcggg ctgctgatgc tcacgaactc gcggcacgtg
     6061 aactcgccct ggctcaagta cccgtcgtac atggtcgaca cgctgctgct gaccgcggcg
     6121 ctgatgctga cgacggtgat ccaccagtac ccgttccagg cgggctggct cacgatgaag
     6181 gtcgtgctgc tggtggtcta cgtcgtgctc ggcagcatcg cgctcaagcg cggccgcacc
     6241 ccgacgatcc gcgtcgtggc gctggtgtcc gcgtggctca cggtcgggtt cctgttcacc
     6301 gtggcacgcg cccaccaccc gctcggtatc ttcgcgatgt cctgaggcat tgcggtcaat
     6361 aggtacaagc cgagagccca atccggcgcg ccgggcgccg cgcgaggcga gcccggttgc
     6421 gcaggctgcg gcacactggt gcccatgacc gtccacgacc ccaacgtgct ggccgggatc
     6481 cgcacccacc acctgttcgc cgggctcgcc ccgccgcagt tgcagcgact gctcgcgacg
     6541 gcacacgtgg aggacgcgga agccgggcag gtgctgttcg accgcggcca gccggcgcga
     6601 cacttctaca tcgtcatcga cggccaggtg aacctggcgc tgtattcgaa gacgggcgag
     6661 gaaaagatcg tcgacatcct gggccccggg cagagcttcg ccgaggcggt catgttcatg
     6721 tcggggcccg tctacccggt ctccgcagtg gccgccgcgc cctcgcgcat cgcacgtttc
     6781 tcgagcaccg actacgtcgc gatgctgcgc gagagcccgg agacgtgcct gcgcatgctc
     6841 gggcacctga gccagcggct gcacatgcgc atccgtgaga tcgagtacct gacgctcgag
     6901 agcgcgacgc atcgcctcgt gcgcttgatc gaggcgcgca tccccgccgg tgcggaagga
     6961 cctgtagagg tgcagctcca ggagtcgcgg caggagatcg cttcgcggct gtcgatgaag
     7021 ccggagacgc tgtcgcgaat cctgcgtcac ctttccgaca acggcgcgat cgaggtgcac
     7081 ggccgcaacc tcgtcgtgcc cgaccccacg cgcctggctt cgatcctgga atcggcgagt
     7141 tgacgccggc gcccgcttga ctgcggtcaa ggcgggcccg tcgtagcgcg cctacagtgg
     7201 gggcatcgac gatcgcagta tcgtccggcg ttccacgagg agaacagcat gggttcgcag
     7261 atcaacggtc gcagcgcccg cctcggcggc tggctcgtcg cgccgctcgc ggcggcgctc
     7321 ggcgtcgccg cctacgcaca acagccatcg acgagcggcg acgcgccgca ggtgcaccag
     7381 gcggaggtgg ccgccggcgc ggcgccgagc gcc
//
LOCUS       DASPFR010000069         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_510301, whole genome shotgun sequence.
ACCESSION   DASPFR010000069 DASPFR010000000
VERSION     DASPFR010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510301"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VD737_01680"
     CDS             complement(<1..592)
                     /locus_tag="VD737_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXW09301.1"
                     /translation="MLEMHNVTKAYRTDLLETHALREFSLEVKQGEFVAVTGPSGSGK
                     TTFLNVAGLLETFDSGTYRLDDVDVSKLDDDARSQVRNRKIGFIFQSFNLIPDLNVFD
                     NVDVPLRYRRMPTAERRDRITAALDLVGLGSRDKHLPSQLSGGQQQRVAIARVLAGAP
                     KLILADEPTGNLDSTMAREIMDILEDINVRGTTLIVV"
     gene            complement(621..1952)
                     /locus_tag="VD737_01685"
     CDS             complement(621..1952)
                     /locus_tag="VD737_01685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXW09302.1"
                     /translation="MPLASITRIANPNRPQGTPGMRSTDGQDRALESRFDWRRDRRVQ
                     VVGGAAVLLLVAVFLTWLLGAWARSDSTVSRDRLRIATVSRGEFLSDVAAQGTVVAAV
                     SPTLYAPAAGTVTLARNAGDAVKKGDLVATVVSPGLRNEFERERATLDSLVVDLRREE
                     IEVRRKILQSQQTSDMAGVAIRAAEREFARAEQAWDQKVISARDYQRARDELDEAKLT
                     HHHAIETAGLEKEGLEFELQTRRLQRDRQKLLVADLERRVGELEVKSPVDGIVGTLAV
                     TQKAAVQQDAAIVTVVDLSALEVEFRVAESYAGAIGLGMPAEVSLGGKTYAGEVTAMS
                     PEVRQGEVTGRVRLKELPPGLRQNQRVSVRILMDERDGVLKVERGAFYEAEGGAYAYV
                     VRDDVAERKPIRTGAVSVREIEIIDGLAEGDQIVISDTDDFKNAERVLLAD"
     gene            2220..>2988
                     /locus_tag="VD737_01690"
     CDS             2220..>2988
                     /locus_tag="VD737_01690"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HXW09303.1"
                     /translation="MDIRGGRRSTNVEDRRGMRIGRTGAGIGIGGVAIAIVLSLLGIN
                     PLPFLEMTQQGSQVEMQPGDQPYQESPREAQYRELAEVVLGETETTWGQQFGGQYTPA
                     RLVLYTEATQSECGTGQAAMGPFYCPLDQRVYVDLSFFDDMQSRFRAGGDFAYAYVIA
                     HEVGHHVQHQLGITQKVEQVRARGNAAQTNNMSVRLELQADCFAGVWGHHAQNARQIL
                     EQGDVEEALNAAAQIGDDRMQMRGRGYVAPESFTHGSG"
BASE COUNT          436 a          984 c         1083 g          485 t
ORIGIN      
        1 tcaccacgat cagggtggtg ccgcggacgt tgatgtcttc caggatgtcc atgatctcgc
       61 gcgccatggt cgagtcgagg ttgccggtcg gctcgtcggc caggatcagc ttgggagcgc
      121 cggccagcac gcgggcgatc gcgacgcgct gctgctgccc gcccgacagc tgcgacggca
      181 ggtgcttgtc gcgggaaccg aggcccacca ggtcgagcgc cgcggtgata cggtcgcgcc
      241 gctccgccgt cggcatgcgc cggtagcgca acggcacgtc gacgttgtcg aacacgttca
      301 ggtcggggat caggttgaag ctctggaaga tgaagccgat cttgcggttg cggacctgcg
      361 agcgcgcgtc gtcgtcgagc ttgctgacgt ccacgtcgtc gagccggtag gtgccgctgt
      421 cgaacgtctc gagcaggcct gcgacgttga ggaacgtggt cttgcccgag cccgacgggc
      481 cggtgacggc cacgaactcg ccctgcttca cctccaggct gaactcgcgc agcgcgtgtg
      541 tttccagcag gtcggtgcgg taggccttcg tgacgttgtg catctcgagc atggaggatt
      601 cccctgtgtg atcgctgcga tcagtccgcg agcagcacgc gctcggcgtt cttgaagtcg
      661 tcggtgtcgg aaatgacgat ctggtcgcct tcggccagcc cgtcgatgat ttcgatctcg
      721 cggacgctga ctgcgcccgt gcggatgggc ttgcgctccg cgacgtcgtc gcggacgacg
      781 taggcgtagg caccgccctc ggcctcgtag aacgcgccgc gctccacctt gagcacgccg
      841 tcgcgctcgt ccatcaggat gcgcacggag acgcgctggt tctggcgcag gcccggcggc
      901 agttccttca ggcgcacgcg accggtgacc tcgccctggc gaacttccgg cgacatcgct
      961 gtgacttcgc ctgcgtacgt cttgccgccc agcgacacct cggccggcat gcccaggccg
     1021 atcgcgccgg cataggattc cgccacgcgg aactcgacct cgagcgccga caggtcgacg
     1081 acggtcacga tcgcggcgtc ctgctgcacg gccgccttct gcgtgaccgc gagcgtgccg
     1141 acgatgccgt cgaccggcga cttgacctcg agttcgccga cgcgacgctc gaggtccgcg
     1201 accagcagct tctggcggtc gcgctgcagg cgacgcgtct gcagctcgaa ctcgaggcct
     1261 tccttctcga ggcccgcggt ctcgatcgcg tggtggtgcg tgagcttcgc ctcgtcgagc
     1321 tcgtcgcggg cgcgctggta gtcgcgcgcg ctgatgacct tctggtccca ggcctgctcg
     1381 gcgcgcgcga actcgcgctc ggcggcccgg atcgcgacgc ccgccatgtc gctcgtctgc
     1441 tggctctgca ggatcttgcg gcggacctcg atttcctcgc ggcgcaggtc gacgaccagg
     1501 ctgtcgagcg tcgcgcgctc gcgctcgaat tcgttgcgaa gtcccgggct gacgacggtg
     1561 gcgaccaggt cgcccttctt gacggcgtcg ccggcattgc gcgcaagcgt gacggtgcca
     1621 gcggccggcg cgtagagcgt cgggctcacg gcggcgacga cggtgccttg ggcggcgacg
     1681 tcgctcagga actcaccgcg cgagaccgtg gcgatgcgca gccgatcgcg cgagacggtc
     1741 gagtcgctgc gtgcccacgc gccgagcagc cacgtcagga acacggcgac gagcagcagc
     1801 accgcggcgc cgccgacgac ctggacgcgc cggtcgcgac gccagtcgaa gcgactctcg
     1861 agcgcgcggt cctggccgtc ggtgctgcgc atgcccggcg tgccctgggg ccggttcggg
     1921 ttggcgatgc gggtgatgga ggcgaggggc atggggagga gtggctagtg gctagtggct
     1981 agtggtcagc ggctggtgcg atggggcgtt ggatgcggcg gacgcggagt tggattgcac
     2041 gacgcgtgcc agctgcgggc ggtttgatca atgccttgat tcggcaaggt tttctgctgc
     2101 cgcgtcatcc agttgggaaa tcccggaccg tccgcttgcg gacagcggac acccgtgcgg
     2161 acacattccg gcgtccgggc ggtcgtgcca tagtccccgc ccgagcgagg agtaaacgca
     2221 tggacatacg cggcgggcgt cgcagcacca acgtcgagga ccggcgcggc atgcgcatcg
     2281 gtcgcacggg tgccggaatc gggatcggcg gtgtcgcgat cgcgatcgtg ctgagcttgc
     2341 tcggcatcaa cccgctgcca ttcctggaga tgacgcaaca gggctcgcag gtcgagatgc
     2401 agccgggcga ccagccgtac caggagtcgc cacgagaagc gcagtaccgc gagctggccg
     2461 aggtggtgct cggcgagacc gagaccactt ggggccagca gttcggcggc cagtacacgc
     2521 cggcccggct cgtgctctac accgaggcga cgcagtcgga gtgcggcacc gggcaggccg
     2581 cgatggggcc gttctactgc ccgctggacc agcgcgtgta cgtcgacctg tcgttcttcg
     2641 acgacatgca gagccggttc cgcgccgggg gcgacttcgc ctatgcgtac gtgatcgcgc
     2701 acgaggtcgg gcaccacgtg cagcaccagc tgggcatcac gcagaaagtg gagcaggtgc
     2761 gggcgcgcgg caacgccgcc cagaccaaca acatgagcgt gcggctggaa ctgcaggcgg
     2821 attgcttcgc gggcgtctgg ggccaccatg cgcagaacgc gcggcagatc ctggagcagg
     2881 gcgacgtgga ggaggcgctc aacgcggctg cccagatcgg cgacgaccgc atgcagatgc
     2941 gcggccgcgg ctatgtcgcg cccgagtcgt tcacccacgg cagcggcg
//
LOCUS       DASPFR010000070         2681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_510515, whole genome shotgun sequence.
ACCESSION   DASPFR010000070 DASPFR010000000
VERSION     DASPFR010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510515"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VD737_01695"
     CDS             complement(<1..1241)
                     /locus_tag="VD737_01695"
                     /inference="COORDINATES: protein motif:HMM:NF023594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXW09304.1"
                     /translation="MSRAGLQSFGAYVPRLRLEREAIAAATGWASGLRGGKPTGRRSY
                     CAWDEDSVTMAVEAARDCLVGHDRAEVAALVFASTTAPFADRCNAGIVAEALDLRESM
                     HTTDGAGHQRAGVAALLDALRNAAIGSEPALIVAAERRVARVGSEQESRFGHGAAAVL
                     VSTGEELAAECVGVVSLRADFVDHYRAADDDYDYAYEERWVRDAGYLALVPRAIQTAL
                     EGAGIAAGDVTHCIVQGPQRFAQAVAKAAGLRGEAVQDDLHGECGDTGVAHPLLVLGA
                     TLERAERGAIVVVAGFGQGCDVVVLRSTGRRVAGGRGASGALAAGIANSEYVRFLANC
                     GLVDVDWGMRAERDNRTAQSALWRHHRDVTAFVGGRCTACGTVQYPRARACVNPVCRA
                     FDTQQPEPLAEKTGRVKTYTE"
     gene            complement(1249..2481)
                     /locus_tag="VD737_01700"
     CDS             complement(1249..2481)
                     /locus_tag="VD737_01700"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HXW09305.1"
                     /translation="MDAASTERFVRRFTEGMQYESTREGPPDGFPRLPPIAGGRYNDP
                     AFLDAEQQGMWKRSWLYACHADEIAEPGSFMLWTKTGSPILIIRGKDRQVRAFYNTCR
                     HRGAPLVKTDTGRCEGLVCSYHGWTYAHDGRLINLRDKRDFVGLDMSQHSLVPVRCEM
                     LANWVFVNENQDARPLLEELGPIAAHLQQFQPEQLRFVEKHGYDLKCNVKVLLDAFLE
                     TYHLKSIHQNTVDRFLDHRGTTIALYRHGHSLMVTPNRRPDWVDPGTVGMRRVETATV
                     ISTRNNPSWNIYPNLVTPIDPSGCPFLVFWPTGPDSMRIECHWFAPDWGDEPLNPLWQ
                     TRIANFDRILDEDLQFAEKIQKSVESPGFRGMTLNYQERRIYHWHEELDRRIAEGGIE
                     LPAHLRVEPRLERYYEDD"
BASE COUNT          407 a          917 c          925 g          432 t
ORIGIN      
        1 tcctcggtgt aggtcttcac gcgtccggtc ttctcggcga gcggttccgg ctgctgggtg
       61 tcgaacgcac ggcacacggg gttcacgcac gcgcgcgcac gcggatactg caccgtgccg
      121 cacgcggtgc accggccgcc gacgaacgcc gtgacgtcgc ggtgatgccg ccacagcgcc
      181 gattgcgccg tgcggttgtc gcgctcggca cgcatgcccc agtcgacgtc gaccaggccg
      241 cagttggcga ggaagcgcac gtactccgaa ttcgcgatcc ctgccgcgag tgccccggag
      301 gccccgcggc caccggcgac gcgacggccc gtgctgcgca gcacgaccac gtcgcagcct
      361 tgcccgaagc ccgcgacgac cacgatcgcg ccgcgctcgg cacgctcgag cgtcgcgccg
      421 agcaccagca gcgggtgcgc cacaccggtg tcgccgcact cgccgtgcag gtcgtcctga
      481 acggcctcgc cgcgcagccc ggccgccttc gccacggcct gcgcgaagcg ctgcggtccc
      541 tgcacgatgc agtgcgtgac gtcgccggcc gcgatgccgg caccctcgag cgccgtttgg
      601 atcgcgcgcg gcacgagcgc cagatagccc gcgtcgcgca cccagcgctc ctcgtatgcg
      661 tagtcgtagt cgtcgtcggc cgcgcggtag tggtcgacga aatcggcgcg cagcgacacg
      721 acccccacgc actcggccgc gagttcctcg ccggtcgaga ccagcacggc cgcggcaccg
      781 tggccgaagc gtgattcctg ctcgctgccg acgcgcgcga cgcgtcgctc ggccgcgacg
      841 atcagggccg gctcgctacc gatggccgcg ttgcgcaggg cgtccagcag cgccgccacg
      901 cccgcgcgct gatggcccgc gccgtcggtg gtgtgcatcg actcgcgcag gtcgagggcc
      961 tcggccacga tgccggcgtt gcagcggtcg gcgaacggcg ccgtggtcga ggcgaagacg
     1021 agcgcagcca cctcggcgcg gtcgtggccc acgaggcagt cgcgcgcggc ctcgaccgcc
     1081 atcgtcacgg agtcctcgtc ccacgcacag taactgcggc ggccggtcgg cttgccgccg
     1141 cgcagtccgg acgcccagcc ggtggccgcc gcgatcgcct cgcgctcgag gcgcaggcgc
     1201 ggcacgtacg caccgaagct ctgcaggccg gctcgactca tcgtcgcctc agtcatcctc
     1261 gtagtagcgt tcgagccgcg gctcgacgcg caggtgcgca ggcagctcga tcccgccctc
     1321 ggcgatgcgg cggtcgagct cttcgtgcca gtgatagatg cgacgctcct ggtagttgag
     1381 cgtcatgccg cggaagcccg gcgattcgac ggacttctgg atcttctcgg cgaactgcag
     1441 gtcctcgtcg aggatgcggt cgaagttggc gatgcgcgtc tgccagagcg ggttcagcgg
     1501 ttcgtcgccc cagtccggcg cgaaccagtg gcattcgatc cgcatcgagt ccgggccggt
     1561 cggccagaac accaggaacg ggcaccccga cggatcgatc ggcgtgacga ggttcgggta
     1621 gatgttccag gacggattgt tcctggtcga gatcaccgtg gccgtctcga cgcggcgcat
     1681 gccgacggtg cccgggtcca cccagtccgg ccggcggttg ggcgtcacca tcaacgaatg
     1741 gccgtggcgg tagagcgcga tcgtcgtgcc gcggtgatcg aggaagcggt cgaccgtgtt
     1801 ctggtggatc gacttgagat ggtaggtctc gaggaacgcg tcgagcagca ctttgacgtt
     1861 gcacttgagg tcgtagccgt gcttttcgac gaagcgcagt tgctccggct ggaactgctg
     1921 caggtgcgcg gcgatcggtc cgagttcctc gagcaacgga cgggcgtcct ggttctcgtt
     1981 gacgaacacc cagttggcga gcatctcgca gcggacgggc accagcgaat gctggctcat
     2041 gtccagcccg acgaaatcgc gcttgtcgcg caggttgatc aggcgcccgt cgtgcgcgta
     2101 cgtccagccg tgatacgaac agaccaggcc ttcgcagcgg cccgtgtcgg tcttgacgag
     2161 cggcgcgccg cgatgccggc aggtgttgta gaaagcgcgg acctggcggt ccttgccgcg
     2221 gatgatcagg atcggggagc ccgtcttggt ccacagcatg aagctgccgg gctcggcgat
     2281 ctcgtcggcg tggcaggcgt acagccacga gcgcttccac atgccctgct gctcggcgtc
     2341 gaggaacgct ggatcgttgt agcgacctcc cgcgatgggg ggcaagcgcg gaaagccgtc
     2401 gggcgggcct tcacgcgtcg attcgtactg catgccctcc gtgaaccggc ggacgaaacg
     2461 ttcggtcgaa gcagcgtcca tcgtgcagat cctctcgagt cgacggacgc agtatagaag
     2521 tcagtacgcc taaaagaaac aaacctgact gtttttttct gagccggtat tgcttatagt
     2581 ccgggtgaac cctgccaagg acacgcggtg accgagccca tcctccacca tttcgactgg
     2641 tcgccgtatg ccgagaaggt gcgcgtgctg ctcggcatca a
//
LOCUS       DASPFR010000071         2706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_998693, whole genome shotgun sequence.
ACCESSION   DASPFR010000071 DASPFR010000000
VERSION     DASPFR010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998693"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1521
                     /locus_tag="VD737_01705"
     CDS             262..1521
                     /locus_tag="VD737_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HXW09306.1"
                     /translation="MADGNGRASQRHWWVLGALAAGIVAGAWIDLAGGPAWHRAAPIL
                     ESVGTLWLNALRMTIGPLVFSLLVVAVASVSDATATGRLASRAIGWFAALLLLAGVLA
                     VVLFEMLLALWPVDRETAAAFLAGVGSATTDAVTTSTWSDWVATVLPPNIIAAAADNA
                     ILALVVFAILFGFAATRLPLPQRDALTTFFGAMADAMVVIVHWVLVVAPVGVFALAVG
                     VGRQAGVGAAGLLLQYAVTVALVIALATLAIYVVVAVRGRLPLRQFTLELAPVQVVAA
                     STQSSLATLPAMLECARGPLGVSPRVASLVLPLAVAVFRYTSPLGNLAVCFFVAAMYG
                     VEPTLWQTVAAITVAIAVSVGSVGLPGQVSFIASVAPICAALGLPIDVLGILVAVEII
                     PDIFRTIGNVTADVAVTTLVDEPPAQA"
     gene            complement(1986..>2706)
                     /locus_tag="VD737_01710"
     CDS             complement(1986..>2706)
                     /locus_tag="VD737_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HXW09307.1"
                     /translation="FGDGPDGHSLWPSFTNVPNEFLESYFPLRIETYETIPDSGGAGK
                     NRGGNGLRIGYRLLEPGEVSIHDDRWLTYPWGVNGGLPGARSRKRLVRKDGTEELLGS
                     KVDRVRVEEGDLLLYDTWGGGGCGDPLEREVERVRFDVEAGLVTPQGARSYGVVVRDD
                     LTVDGKATEALRAQMRTERGPVPLFDRGFESIEELKARCLDETGLAPPEQPRFADRAL
                     ARKSAAATVATPKRKVKRKAA"
BASE COUNT          337 a          978 c          955 g          436 t
ORIGIN      
        1 tccagggtcg accgaaaaag gtgcgccact ttaccaccgg agcccgccgg cacgccggcg
       61 gggtcgcccg ttgccgccgc aacggcggca atccccacgc ccgccccgcg tgcagcgcag
      121 cccgctttgc cgggcaccga gcggtgtgga atgatcgatt cgccacgcga ccgccgcggg
      181 cacacccgga gcgcacgcgg ccgatggcca gacctgtcga agacgcagca attaccctgg
      241 cggcgttcca ggacgctggc ggtggctgac gggaacggcc gggcctccca acggcactgg
      301 tgggtgctcg gcgccctcgc ggccggcatc gtcgccggcg cctggatcga cctcgccggg
      361 ggaccggcgt ggcatcgtgc tgcgccgatc ctcgagagcg tcggcacgct ctggctgaac
      421 gcgctgcgca tgacgattgg tccgctcgtg ttctcgctgc tcgtggtcgc cgtcgcgagc
      481 gtctccgacg cgaccgcgac gggccggctc gcgagccgcg caatcggctg gttcgccgcc
      541 ttgctgctgc tcgccggcgt gctcgcggtg gtgctgttcg agatgctgct ggcgctctgg
      601 ccggtcgatc gcgaaacggc cgcggcgttt ctcgcgggcg tcggcagcgc gacgaccgac
      661 gcggtcacga cctcgacctg gagcgactgg gtcgcaaccg tgctgccacc gaacatcatc
      721 gcggcggcgg cggacaacgc gatcctggcg ctggtcgtgt tcgccatcct gttcggcttc
      781 gcggcgacgc gcttgcccct gccgcaacgc gacgcgctga cgactttctt cggcgcgatg
      841 gccgacgcga tggtcgtgat cgtgcactgg gtgctggtcg tcgctccagt cggcgtcttc
      901 gcactcgcgg tcggcgtcgg ccgccaggcg ggcgtcggcg cggccggact gctgctgcag
      961 tacgcggtca ccgtcgcgct ggtgatcgcg ctggcgacgt tggcgatcta cgtcgtcgtc
     1021 gcagtgcgcg gacgcttgcc gctccggcag ttcacactcg agctcgcgcc ggtgcaggtc
     1081 gtggccgcca gcacgcagag ctcgctcgcg acgctgccgg ccatgctcga gtgcgcgcga
     1141 gggcccctcg gcgtttcacc gcgcgtggcg agcctggtgc tgccgctcgc ggtcgcggtg
     1201 ttccggtaca cgagtccctt ggggaacctc gccgtctgct tcttcgtcgc cgcgatgtac
     1261 ggcgtcgagc cgacgctctg gcagacggtc gccgccatca cggtcgcgat cgcagtcagc
     1321 gtgggctcgg tcggactgcc gggccaggtg tcgttcatcg cgagcgtcgc cccgatctgc
     1381 gccgcgctcg ggctgccgat cgacgtgctc ggcatcctgg tcgcggtcga gatcatcccg
     1441 gacatcttcc gcacgatcgg caacgtgacg gcggacgtcg cggtgacgac gctggtcgac
     1501 gagccgccgg cgcaggcgta acgccgcagc cgtgcgactg cctcaggcgg gacgtgcgcg
     1561 tccccgggcc gccgtcgacg ccgtatgcgc cgaggtgact gcgacggcgc cggtctccgg
     1621 gttcggtccg ggagagacgc cggcaagacg catccatgcg ggctcggcac gggccgtcca
     1681 tggcccgtga cgctctcccg gaccgaaccc aaagcccagc gctcgtcgct cctggcgctg
     1741 gtgcagttgt tggtgcgtgc cgggaagcac gggcgcgagc agatgtgcag gccgcgacgc
     1801 ggagcgcgtc tgacgcttcg cggcgcgtcc tgcgcgccgc acgggtcggg tccggatcca
     1861 gtccgcaaga gcgtcacggg ccatggacgg cccgtgccga gcccgcatgg atgcgtcttg
     1921 ctggcgtctc ttgcggactg gatgcggatc cggaccgaag gcgcgctcaa gctaccgagt
     1981 cggtatcacg ctgctttgcg cttgaccttg cgcttaggcg tcgcaacggt ggccgccgcc
     2041 gactttcgcg cgagcgcgcg atccgcgaag cgcggctgct ccggcggcgc gaggccggtc
     2101 tcgtcgaggc agcgcgcctt gagttcttcg atcgattcga agccgcgatc gaagagcggc
     2161 accggcccgc gctcggtgcg catctgcgca cgcagcgcct ccgtcgcctt accgtcgacc
     2221 gtcaggtcgt cgcgaacgac gacgccgtag ctgcgcgcgc cctgcggcgt gacgagcccc
     2281 gcctcgacgt cgaagcgtac gcgttccact tcgcgctcga gcgggtcgcc gcaaccgcca
     2341 ccgccccagg tgtcgtagag cagcaggtcg ccctcctcga cccggacgcg atcgaccttc
     2401 gagcccagca gttcctccgt gccgtccttg cgcaccagcc gcttgcgact gcgcgcgccc
     2461 ggcagcccgc cgttgacgcc ccaggggtac gtgagccagc ggtcgtcgtg gatcgagacc
     2521 tcgccaggct cgagcaatcg gtagccgatc cgcagtccgt tgccaccgcg gttcttgccc
     2581 gcgccgcccg agtccgggat cgtctcgtac gtctcgatcc gcaacgggaa gtagctctcc
     2641 aggaactcgt tcggtacgtt cgtgaacgac ggccacagcg agtgtccgtc cggcccgtcg
     2701 ccgaac
//
LOCUS       DASPFR010000072         2997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_22854, whole genome shotgun sequence.
ACCESSION   DASPFR010000072 DASPFR010000000
VERSION     DASPFR010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22854"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VD737_01715"
     CDS             complement(<1..524)
                     /locus_tag="VD737_01715"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXW09308.1"
                     /translation="MADTLLARTPRDQLPEQFRLAWDTMNRLTGEPTFVEVFAANPDM
                     LDFVMNRFYAGIFFGGRVDQRYKQLVRLELSLLHGCRTCNKQNVPGALEAGITQAQVD
                     AMLDFETGPFTDAEKAVIAYAREVALTNPEGRMTPELHRRLSAHFSQADILELGVVMA
                     VVSGMAKLSFVLDLV"
     gene            complement(548..1441)
                     /locus_tag="VD737_01720"
     CDS             complement(548..1441)
                     /locus_tag="VD737_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXW09309.1"
                     /translation="MNNNIKRLEPAAEPRQWGLDDEALATRPTVFRDGLLDGRVFIVS
                     GGGSGIGRGITYLLARLGADVMICGRRPEKLEETAGGIKRLLGRDVATCAMTIREPDQ
                     VESLVAQTFERFGRLDTLVNNGGGQFPQAAIDFSVKGWNAVIDTNLNGTWYMMQSAAK
                     QWRERGTAGNIVNIVATVARGMPQVAHTCAARAGVIYLSKTVATEWAPLGIRVNCVAP
                     GSIATEGFNVYPDGVPDRFALTNPMKRLGEVHDVAEAVVYIAADSGKFVTGEVLTVDG
                     GHSVWGDTEWPGGKPPYFDVD"
     gene            complement(1446..2648)
                     /locus_tag="VD737_01725"
     CDS             complement(1446..2648)
                     /locus_tag="VD737_01725"
                     /inference="COORDINATES: protein motif:HMM:NF004810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HXW09310.1"
                     /translation="MARGIKDKVAIVGMGCSRFGERWDCSPEDLMVEAFGEALQDAGI
                     ERDRIEAAWLGVFYQEQSVSRSGLPLSIGLKLPNIPVTRVENLCATGTEALRGAAYAV
                     AAGAVDIALAFGVEKLKDTGFAGLPERTKGTFEDLWQPGFTPPGAFAQLGAAYAARHG
                     HRIDDLKRAMAHVSWKSHENAATNPKAHLRNRITIDQALAAPMVAYPLGLYDCCGVSD
                     GAACAIVTTPEFAASLGKRDMVTIKALALAPSNGVEMGHASWDGAHTLTTRIAAERAY
                     REAGITDPAAQIDLMEVHDCFSITELVLMEDLGFSAPGRAVHDVLDGRYDRDGEVPCQ
                     TDGGLKCFGHPVGASGLRMAYEIYTQLLGRAGDRQLESPSLGLTHNLGGIPNRNVASV
                     SVLGLHRG"
     gene            complement(2651..>2997)
                     /locus_tag="VD737_01730"
     CDS             complement(2651..>2997)
                     /locus_tag="VD737_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HXW09311.1"
                     /translation="GKCTACGTVQYPRTRACVNPACRAFDTQEPEPLAEKTGRVKTYT
                     EDWLAVTRSPPHVYGNVEIDGGGNAFIEFADTRPGELAVGAPVRFVFRVKDFDTVRGF
                     RRYFWKAVPVRN"
BASE COUNT          428 a         1034 c         1038 g          497 t
ORIGIN      
        1 acgagatcga gcacgaacga gagcttggcc atgcccgaaa cgacggccat cacgacgccc
       61 agctcgagga tgtcggcctg cgagaaatgc gcggacaggc gccggtgcag ttcgggcgtc
      121 atgcggccct cggggttggt cagggccacc tcgcgcgcat acgcgatcac ggccttttcg
      181 gcgtccgtga acggcccggt ctcgaagtcg agcattgcgt ccacctgggc ctgggtgatg
      241 ccggcctcga gcgcgccagg cacgttctgc ttgttgcagg tccggcagcc gtgcagcagc
      301 gagagctcga gcctcaccag ctgcttgtag cgctggtcga cacggcctcc gaagaagatg
      361 ccggcgtaga agcggttcat gacgaagtcg agcatgtcgg ggttcgcggc gaacacctcg
      421 acgaacgtcg gctcgccggt caggcggttc atggtgtccc aggcgagccg gaactgctcg
      481 gggagctgat cccgcggggt gcgggcaagg agtgtgtcgg ccacgctgtt gcctcctgct
      541 gtggtcgtca gtcgacgtcg aagtacggcg gtttcccgcc cggccattcc gtgtcgcccc
      601 agacgctgtg cccgccgtcg acggtcagca cctcgcccgt gacgaacttg cccgaatccg
      661 cggcgatgta gacgaccgcc tccgcgacgt cgtggacctc gcccagccgc ttcatcgggt
      721 tggtcagcgc gaagcggtcg ggcacgccgt cggggtagac gttgaagccc tccgtcgcga
      781 tcgagcccgg cgcgacgcag ttgacgcgga tgccgagcgg cgcccattcg gtcgcgacgg
      841 tcttgctcag gtagatcacg ccggcacgcg cggcgcacgt gtgggccacc tgcggcatgc
      901 cgcgagcgac cgtggccacg atgttgacga tgttgccggc cgtgccgcgc tcgcgccact
      961 gcttcgcggc cgactgcatc atgtaccacg tgccgttcag gttggtgtcg atgaccgcat
     1021 tccagccctt gacgctgaag tcgatcgccg cctgtggaaa ctgcccgccg ccgttgttga
     1081 ccagcgtgtc gagccgcccg aagcgctcga acgtctgcgc gaccagcgac tcgacctggt
     1141 cgggctcgcg gatcgtcatc gcgcaggtcg cgacgtcgcg tccaagcagg cgcttgatgc
     1201 cgcccgccgt ctcctcgagc ttctccgggc gtcgcccgca gatcatcacg tcggcgccca
     1261 ggcgggcgag caggtaggtg atgccgcggc cgatgccgct gccgccgccc gacacgatga
     1321 acacccggcc gtccagcagc ccgtcacgga acacggtcgg gcgcgtggcc agtgcctcgt
     1381 cgtcgaggcc ccattgccgc ggctcggcgg caggctccag gcgcttgatg ttgttgttca
     1441 cccgatcaac ctctgtggag tccgagcacc gacacgctcg cgacgttgcg attggggatg
     1501 ccgcccaggt tgtgcgtgag gccgagcgac ggcgactcga gctgccggtc cccggcgcgg
     1561 ccgagcagct gcgtgtagat ctcgtacgcc atgcgcagtc ccgaggcgcc gaccggatgc
     1621 ccgaagcact tcaggccgcc gtccgtctgg cacggaacct cgccgtcgcg atcgtagcgg
     1681 ccgtcgagca cgtcatgcac ggcgcgcccg ggcgcggaga agccgaggtc ctccatcagc
     1741 acgagctccg tgatcgagaa gcagtcgtgc acttccatga ggtcgatctg cgcggccgga
     1801 tccgtgatgc cggcttcgcg ataggcgcgc tccgccgcga tccgcgtggt gagcgtgtgc
     1861 gcaccgtccc agctcgcgtg gcccatctcg acgccgttcg acggcgcgag cgcgagcgcc
     1921 ttgatcgtga ccatgtcgcg cttgccgagc gacgcggcga actccggcgt cgtgacgatc
     1981 gcgcaggccg cgccgtcgct gacgccgcag cagtcgtaca gtccaagcgg gtaggcgacc
     2041 atcggtgcgg cgagcgcctg gtcgatcgtg atccggttgc gcaggtgcgc tttcgggttg
     2101 gtcgcggcgt tctcgtggct cttccacgag acgtgggcca tcgcgcgctt caagtcgtcg
     2161 atgcggtggc cgtgccgcgc ggcgtacgcg gcgccgagtt gtgcgaacgc gcccggcggt
     2221 gtgaacccgg gctgccagag gtcctcgaac gtgcccttcg tgcgttcggg caggcccgcg
     2281 aacccggtgt ccttgagttt ctcgacgccg aacgcgagcg cgatgtcgac cgcgccggcg
     2341 gccacggcgt aggccgcgcc gcgcaatgcc tccgtgcccg tcgcgcagag gttctcgact
     2401 cgggtcaccg ggatgttcgg caatttcagg ccgatcgaca gtggcaggcc cgaccggctg
     2461 acgctctgct cctggtagaa cacgccgagc caggcggcct cgatgcggtc gcgctcgatg
     2521 ccggcgtcct gcaaagcctc gccgaaggcc tcgaccatga ggtcctcggg actgcaatcc
     2581 cagcgctcgc cgaaccgcga gcaccccatg cccacgatcg cgaccttgtc cttgatgccg
     2641 cgtgccacgg tcagttcctc accggcacgg ctttccagaa gtagcgccgg aacccgcgca
     2701 ccgtgtcgaa gtccttgacg cggaacacga agcgcaccgg ggcaccgacc gcgagttcgc
     2761 ctggacgggt gtcggcgaac tcgatgaagg cattgccgcc gccgtcgatc tcgacgttgc
     2821 cgtacacgtg cggcgggctg cgcgtgaccg cgagccagtc ctccgtgtag gtcttcacgc
     2881 gtcctgtctt ctcggcgagc ggttccggtt cctgggtgtc gaacgcgcgg caggccgggt
     2941 ttacgcaggc ccgcgtgcgc gggtactgca cggtgccgca ggcggtgcac ttgccgc
//
LOCUS       DASPFR010000073         6644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1731182, whole genome shotgun sequence.
ACCESSION   DASPFR010000073 DASPFR010000000
VERSION     DASPFR010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731182"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1025)
                     /gene="rsmI"
                     /locus_tag="VD737_01735"
     CDS             complement(129..1025)
                     /gene="rsmI"
                     /locus_tag="VD737_01735"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007989873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HXW09312.1"
                     /translation="MSDRNVDSTGRRTAGTLFVVATPIGNLQDLSPRAAACLRDADLL
                     LAEDTRHTRQLLEACGISRSGALESLHEHNERQRVPALLARLQHGESVALVTDAGTPL
                     MSDPGATLVSAAADAGLAVVAVPGACAAIAALSIAGLPTDRFAFEGFLPAKTGARREV
                     LQRLSSEPRTLVFYEAPHRLVDTLRDLADVFGGERKGVVARELTKRFESTYRGTLADL
                     AQRSSADADMTRGELVILVTGAPPASDDADASREAERVLRALLAELPVSQAARLAAEL
                     TGRSRSELYERALALKGEGGSG"
     gene            1049..2986
                     /locus_tag="VD737_01740"
     CDS             1049..2986
                     /locus_tag="VD737_01740"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HXW09313.1"
                     /translation="MLHVWLRRGQAALRAPLVALAIGATALQAPAPAQGQSADVAAEQ
                     AASLAASGRHLEAAARYEQAAKPRFLAPWDARYALLAAREYVAAGEFQEAERLLDKVR
                     SRVRTDEERALLALVVGNLALDRGDARAALAPLRAAPMSIPTDLAVDVLESRGRAEIA
                     SGEALAGVRTFEARGAMLADDAARRDNDRRLFDQLLLHPPAAAVPAIPGMSERERGWL
                     ELPGIVAGSAGASSTQAAFRVRDWLAQHPGHPATGFLPRVGSAVLTAPMSGDASIALL
                     LPLSGRQHAAAAAVRDGFTAAWFASGSGETRTRVDVYDTTSGVSAAYQRAIADGASVV
                     AGPLLKDEVLALVAAQPSGLPVPTLALNSAIPEGSPVPTFLFQFALDPEQEARAVARR
                     IVDDGLTRGIGLFPEGAWGRRIHAAFVDELTRLGSVALASAQFYAPEAQDFSAPLRAA
                     LGRFGGAGDRPADRRQPRASRDAAAEQAAGPQFAFLAATPATARAIRPQLRFQMTYDL
                     PVYATSDAWDPGARAAADLEGLLFPEMPWLLYAGQGAPELWDAAQNEWSSRVRGRLRL
                     FAFGYDAFRLAQQLGRGPGVAGLEGLTGTLEIQRGDGHVRRSLQFARVTNGRPVPALP
                     GPPSAFPETPPANAGGTGLPR"
     gene            3027..3479
                     /locus_tag="VD737_01745"
     CDS             3027..3479
                     /locus_tag="VD737_01745"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HXW09314.1"
                     /translation="MHDPSAADDEDVDQDRDGGDPDGAPDTTTVRGRRAEDLALRQLQ
                     HAGLQLVTRNYRCRGGEIDLVMFEPSTRTLVIVEVRSRARGDFGSAASTIGFAKQRRI
                     ILAARHLLLARRELRHLRARFDVVALDPPARSQGAWAVTWIRGAFGSV"
     gene            complement(3574..4065)
                     /locus_tag="VD737_01750"
     CDS             complement(3574..4065)
                     /locus_tag="VD737_01750"
                     /inference="COORDINATES: protein motif:HMM:NF008769.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HXW09315.1"
                     /translation="MAAPPSPPMKPRRPYLLRAIHEWITDSLCTPHLVVDASVKGVEV
                     PRQYVKDGKIVLNVSWNATANLRLGNEELTFSGRFGGTSMSLCIPIQAVLAIYARETG
                     QGMIFTDEDAVEAPASAKEPTTGDASTSADTPGTLDEGGGGDRPPPRRPSGGGRAKLK
                     VVK"
     gene            complement(4179..4811)
                     /locus_tag="VD737_01755"
     CDS             complement(4179..4811)
                     /locus_tag="VD737_01755"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HXW09316.1"
                     /translation="MMTLYSRSDDARCHRVRIVLAEKGLDVRIVETDPARPPEDLIDL
                     NPYQTVPTLVDRDLVVYEPGIIAEYLDERFPHPTLASTDPAVRAQARVALRRIEQDWY
                     AVADALEPGLGNERRERERARKMLAESILASEPLFRVRPWFLSEQFSQLDAAVAPILW
                     RLPRWEIDLTAVGSAAPAIERYAAKAFARPSFVRSLSEAEKAMRRKVVGG"
     gene            complement(4849..5589)
                     /locus_tag="VD737_01760"
     CDS             complement(4849..5589)
                     /locus_tag="VD737_01760"
                     /inference="COORDINATES: protein motif:HMM:NF014247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HXW09317.1"
                     /translation="MKLLALIASMLAFPAGSYAAGGETHVEHAGNDIHNVASLQRGAR
                     NFVNYCLGCHSAKYVRYNRMAADIGLSEQQMIDNLMFTGDSPHQTMTNGMNPDDARRW
                     FGVAPPDLSLIARSKGPDYVYTFLRSFYADPTKATGVNNLTLPGTAMPHVLWELQGMQ
                     QAVWEGHTDAQGNVQKQFKEFELAEPGKLSPEQYDAFVRDTVNFLQYIGEPVQVRRQA
                     LGYVVISFLVFFTLLAYALKKEYWKDVK"
     gene            complement(5600..>6644)
                     /locus_tag="VD737_01765"
     CDS             complement(5600..>6644)
                     /locus_tag="VD737_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014592292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HXW09318.1"
                     /translation="KPSSAEAFASVEYIMRDVEWGWLIRYLHSTGASAFFVVVYLHMF
                     RALIYGSYQKPRELLWIFGMLIYFALMAEAFMGYLLPWGNMSYWGAQVIVSLFGAIPY
                     VGDSLTEWIRGDYYISDVTLNRFFALHVVAIPFALVFLVIAHLMALHEVGSNNPDGVE
                     IKKVKGPDGLPLDGIPFHPYYTVKDMVGVGVFLILFSLVIFFAPTMGGYFLEYANFDP
                     ANPLQTPPHIAPVWYFTPFYAILRAVPSKSLGALLMGAAVLLFVFLPWLDRGKVRSIR
                     YRSPVFKFFVIAFAISFLALGWLGLQPATPGYTMLARAFSVVYFAFFLLMPFYTRNER
                     TKPVPTRVTYHAH"
BASE COUNT          994 a         2292 c         2351 g         1007 t
ORIGIN      
        1 aagagcgtgc tcgggctcaa gtgaggaagc ggttggcggt tagcggttag tgaacagtcc
       61 cagcccaaac gccgttgggt tgctgctgcg gcgaaaatag gttccgacta accgctaacc
      121 gctaaccgct aaccgcttcc cccttcgccc ttcagcgcca gtgcccgctc gtacaactcg
      181 ctgcggctgc gtccggtcag ttccgcggcg aggcgcgccg cctgcgacac cggcagttcg
      241 gcgagcagcg cgcgcaacac gcgctcggcc tcgcgtgagg cgtccgcatc gtcggacgcg
      301 ggcggtgcgc cggtgacgag aattacgagt tcgccgcgcg tcatgtcggc gtcggcggag
      361 ctgcgctggg caagatcggc gagcgtgccg cggtacgtgc tttcgaagcg cttggtcagc
      421 tcgcgagcga cgacgccctt gcgctcgccg ccgaagacgt ccgcaaggtc gcgcagcgtg
      481 tctacgagtc gatgcggcgc ctcgtagaac accagcgtgc gcggttcgga cgagaggcgt
      541 tgcagcactt cgcgacgcgc gcccgtcttc gccggcagaa atccctcgaa cgcgaagcga
      601 tccgtcggca atcccgcgat cgacaacgcg gcgatggcgg cgcacgcgcc cggcaccgcg
      661 accacggcga gccctgcgtc cgcggcggcg ctcacgagcg tggcaccggg atcgctcatc
      721 agcggcgtgc cggcatccgt caccagcgcg accgactcgc cgtgctgcag gcgcgcgagc
      781 agtgccggca cgcgctggcg ctcgttgtgc tcgtgcagcg attccagtgc gccgctgcgt
      841 gaaatgccgc aggcctcgag cagttgccgc gtgtgacgcg tgtcttccgc gagcagcagg
      901 tcggcgtccc gcaggcaggc cgctgcgcgg ggactgagat cctgcaggtt gccgatcggc
      961 gtcgcgacga cgaacagtgt gccggccgtg cggcggcccg tagaatcaac gttcctgtcc
     1021 gacacgcgcg cctcctcgag gtccctgcat gctgcacgta tggctccgtc gcgggcaagc
     1081 cgcgctccgc gcgccgttgg tcgcgctggc catcggcgcg acggcgctgc aggctccggc
     1141 gccggcgcag gggcagtccg cggacgtcgc ggccgagcag gccgcgtcgc tcgcggcgtc
     1201 agggcgtcat ctcgaggcgg ccgcgcgcta cgagcaggcg gccaagcccc ggttcctcgc
     1261 gccgtgggac gcgcgctacg ccctgctcgc agcccgcgaa tacgtggcgg ccggcgagtt
     1321 ccaggaggcc gaacggctgc tcgacaaggt gcgctcgcgc gtgcgcaccg acgaggagcg
     1381 cgcactgctc gcgctggtcg tcggcaacct ggcgctcgat cgcggcgacg cgcgcgcggc
     1441 gctcgccccg ttgcgcgcgg cgccgatgag cataccgacg gacctcgcgg tcgacgtgct
     1501 ggagtcgcgc ggacgggccg agatcgcgag cggcgaggca ctggcgggcg tgcgcacgtt
     1561 cgaggcgcgc ggcgccatgc tggccgacga cgccgcccgg cgcgacaacg accgacggct
     1621 gttcgaccag ttgctgctgc atccgccggc cgcggccgtg cccgccatcc ccggcatgtc
     1681 cgagcgcgaa cgtggctggc tcgagctgcc gggcatcgtg gcgggctcgg ccggcgcgtc
     1741 ttcgacgcaa gcggctttcc gcgtgcgcga ctggctggcc cagcaccccg gccatccggc
     1801 caccggcttc ctgccccgcg tcggcagcgc ggtactgacg gcgccgatga gcggggacgc
     1861 ctcgatcgcg ctgctgctgc cgctgtcggg tcggcagcac gccgcggccg cggccgttcg
     1921 cgacggtttc accgcggcct ggttcgcgtc cggttccggc gaaacgcgca cccgggtcga
     1981 cgtgtacgac acgacgagcg gggtgtccgc ggcgtaccag cgcgcgatcg cggacggcgc
     2041 gagcgtcgtc gccggtccgc tgctcaagga cgaggtgctc gcgctggtcg ccgcgcagcc
     2101 ttcggggctg ccggtgccga cgctcgcgct caattcggcg atcccggagg gctcgccggt
     2161 gccgaccttc ctgttccagt tcgcgctcga tccggaacag gaagcgcgcg ccgtggcgag
     2221 gcgcatcgtc gacgacgggt tgacgcgcgg catcggactg tttccggaag gcgcctgggg
     2281 ccggcgcatt cacgcggcgt tcgtcgacga gctgacgcgt ctcggttccg tcgcgctggc
     2341 ctcggcgcag ttctacgcgc ccgaggccca ggacttctcg gcaccgttgc gggcggccct
     2401 cgggcgcttc ggtggcgcag gcgaccgacc ggcagaccgc cgccagccgc gtgcctcgcg
     2461 cgacgccgcg gccgagcagg ccgccggtcc gcagttcgcc ttcctggcgg ccaccccggc
     2521 gacggcgcgt gcaattcggc cgcagctgcg cttccagatg acctatgacc tgcccgtgta
     2581 tgcgacgtcc gacgcgtggg atcccggcgc gcgtgccgcg gccgacctgg agggcctgct
     2641 gtttccggag atgccgtggc tgctgtacgc cggccagggc gcgcccgagc tctgggacgc
     2701 ggcacagaac gagtggtcct cgcgggtgcg cggtcgcctg cgcctcttcg cgttcggcta
     2761 cgacgctttc cggctcgcgc aacagctcgg gcgaggcccg ggcgtggcgg gcctcgaagg
     2821 cctgaccggc acgctcgaga tacagcgcgg cgacgggcac gtgcgtcgca gcctgcagtt
     2881 cgcccgcgtc accaacggcc ggcccgtgcc ggcgctcccg ggaccgccgt ccgcgtttcc
     2941 ggagacaccg cccgcaaatg cgggcggcac ggggctgccg cgctgagtac aacggcaggt
     3001 ctcgcgggac ctggcgtctg gacaccgtgc acgacccctc agcggccgat gacgaggacg
     3061 tcgatcagga ccgcgacggc ggtgatcctg acggcgcccc tgacacgacc accgtgcgcg
     3121 gccgccgcgc ggaagacctg gcgctgcgtc agctgcagca cgccgggctc caactggtga
     3181 cgcgcaacta ccgctgccgc ggcggcgaga tcgacctggt catgttcgag ccgtcgacgc
     3241 gcacgctggt gatcgtggaa gtgcgctccc gggcccgcgg cgacttcggc agcgccgcgt
     3301 cgacgatcgg tttcgccaag cagcggcgca tcatcctcgc cgcccgccac ctgctgctgg
     3361 cgcggcgcga gctgcggcac ctgcgtgcac gcttcgacgt cgtcgcgctc gacccgccgg
     3421 cgcgatcgca gggcgcgtgg gcggtgacgt ggatcagggg agccttcgga agtgtctagt
     3481 gtctagtgtc cagtgttcag taagaaggcg gctgcgtcgc cgccgatgca gtcggcttcc
     3541 tactaaccgc tagacactga acactagaca cttctacttg acgactttca gcttcgcccg
     3601 accgccgccg gacggacgac gcggcggcgg ccggtcgccc ccgccaccct cgtcgagtgt
     3661 gcccggcgta tccgccgacg tcgaagcgtc gcccgtcgtc ggctccttgg ccgaagccgg
     3721 tgcttccacg gcgtcctcgt cggtgaagat catgccctgc cccgtctccc gcgcgtagat
     3781 cgcgagcacg gcctggatcg ggatgcacaa cgacatgctc gtgccgccga agcgcccgct
     3841 gaacgtgagt tcctcgttgc cgagccgcag gttggccgtc gcgttccagc tcacgttcag
     3901 cacgatcttg ccgtccttga cgtactgacg cggcacctcg actcccttca cgctggcatc
     3961 gacgacgagg tgcggcgtgc acaggctgtc ggtgatccac tcgtggatgg cgcgcagcag
     4021 gtaaggacgc cgtggcttca tcggcgggga gggaggtgca gccatgcccg aagtatgggc
     4081 ggaccgggcg agaagtggct agtggcgggt ggcttggtac ggaacgccga gcccggcgtc
     4141 agctccggac cttctcgttc ctcaccgacc accagccact agccaccaac cactttcctc
     4201 cgcatcgctt tttccgcttc gctcaggctg cgcacgaacg acggacgcgc gaacgccttg
     4261 gccgcatagc gttcgatcgc gggcgcggcc gacccgaccg cggtcaggtc gatttcccag
     4321 cgcgggaggc gccacaggat cggcgcgacg gcggcgtcga gctgcgagaa ctgctccgac
     4381 aggaaccagg gacgcacgcg gaacagcggc tccgaggcga ggatgctctc ggccagcatc
     4441 ttgcgggccc gctcgcgctc gcggcgctcg ttgccgagac cgggttcgag cgcgtcggcg
     4501 acggcgtacc agtcctgctc gatgcgacgc agcgcgaccc gcgcctgcgc gcgcaccgcc
     4561 ggatcggtcg aagcgagcgt cgggtgcggg aaccgttcgt cgagatactc cgcgatgatg
     4621 ccgggctcgt agacgacgag gtcgcggtcg accagcgtcg gcaccgtctg gtacgggttc
     4681 aggtcgatca ggtcctcggg tggacgcgcc gggtcggtct cgacgatgcg cacgtcgagt
     4741 cccttttcgg ccagcacgat ccgcacgcga tggcagcgcg cgtcgtccga ccgcgagtag
     4801 agcgtcatca tcctgacggc gcctgctgcc ggggccgcac ggggcggctt acttgacgtc
     4861 cttccagtat tccttcttca gcgcgtacgc gagcagcgtg aagaacacga ggaacgagat
     4921 cactacgtag ccgagcgcct gtcgccgcac ctgcaccggc tcgccgatgt actggaggaa
     4981 gttgacggtg tcgcgcacga acgcgtcgta ctgctccggc gacagcttgc ccggctcggc
     5041 gagctcgaac tccttgaact gcttctgcac gttgccctgc gcgtccgtgt ggccttccca
     5101 gaccgcctgc tgcatgccct gcagttccca cagcacgtgc ggcatcgcgg tgcccgggag
     5161 cgtcaggttg ttgacgccgg tggccttggt cggatcggca tagaacgagc gcaggaacgt
     5221 gtagacgtaa tcgggcccct tgctgcgggc gatcagcgac aggtccggcg gggcgacgcc
     5281 gaaccagcgg cgggcgtcgt cggggttcat gccgttcgtc atcgtctggt gcggcgagtc
     5341 gcccgtgaac atcaggttgt cgatcatctg ctgctcggac aggccgatgt cggccgccat
     5401 gcggttgtag cgcacgtact tggcggagtg acagccgagg cagtagttga cgaagttccg
     5461 ggccccgcgt tgcagcgagg cgacgttgtg gatgtcgttg ccggcgtgct cgacgtgcgt
     5521 ctcgccgccc gcggcgtacg agcccgccgg aaacgcgagc atcgacgcga tgagggcgag
     5581 gagcttcatc ctgtgacggt cagtgcgcat ggtaggtcac cctcgtcggc acgggcttgg
     5641 tccgctcgtt gcgcgtgtag aacggcatca gcaggaagaa cgcgaaatag accaccgaga
     5701 acgcacgcgc cagcatcgtg tagcccggcg tcgcgggctg caggccgagc cagccgagcg
     5761 cgaggaagct gatcgcgaac gcgatcacga agaacttgaa gaccgggctg cggtagcgga
     5821 tcgacctgac cttgccgcgg tcgagccacg gcaggaacac gaacagcagt acggccgcgc
     5881 ccatgagcag ggcacccagc gacttgctcg ggaccgcgcg caggatcgcg tagaacggcg
     5941 tgaagtacca gaccggcgca atgtgcggcg gcgtctggag cgggttcgcc gggtcgaagt
     6001 tcgcgtactc gaggaagtac ccgcccatcg tcggcgcgaa gaagatcacg agcgaaaaca
     6061 ggatcaggaa gacgccgacg ccgaccatgt ccttgaccgt gtagtacggg tggaacggga
     6121 tcccgtcgag cggcaggccg tccgggccct tgaccttctt gatctcgacg ccgtccggat
     6181 tgttcgagcc gacctcgtgc agcgccatca ggtgcgcgat caccaggaac accagtgcga
     6241 acgggatcgc gaccacgtgc agcgcgaaga agcggttcag cgtgacgtcc gagatgtagt
     6301 agtcgccgcg gatccactcg gtgagcgagt cgcccacgta cgggatggcg ccgaacagtg
     6361 acacgatcac ctgcgcgccc cagtacgaca tgttgcccca cggcagcagg tagcccatga
     6421 acgcctcggc catcagcgcg aagtagatca gcatgccgaa gatccacagc agttcgcgcg
     6481 gcttctggta cgagccgtag atcagcgcgc ggaacatgtg caggtagacg acgacgaaga
     6541 aggccgaggc gccggtcgag tgcagatagc ggatcagcca gccccactcg acgtcgcgca
     6601 tgatgtactc gaccgaggcg aacgcctccg ccgaggacgg cttg
//
LOCUS       DASPFR010000074        11235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_144824, whole genome shotgun sequence.
ACCESSION   DASPFR010000074 DASPFR010000000
VERSION     DASPFR010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11235
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144824"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1940)
                     /gene="aceE"
                     /locus_tag="VD737_01770"
     CDS             complement(<1..1940)
                     /gene="aceE"
                     /locus_tag="VD737_01770"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HXW09319.1"
                     /translation="MFEASKIEDADPDETREWLESIDSVLKTHGAERAQYLLERVIDF
                     TRRSGAYLPFKPNTAYVNTISRAQEQPYPGDRGLERRIEAYLRWNAMAMVVQANRQSS
                     EFGGHIASYASAATLYEVGFNHFWRAPSDRHPGDMVFVQGHSAPGIYARAYLEGRLTE
                     DHLRLFREEVGGGGLSSYPHPWLMPEFWQFPTVSMGLGPMMAIYQARFIRYLEHRGIV
                     PPSDRKVWTFLGDGEMDEPESLGAITMPVREKLDNLVFVINCNLQRLDGPVRGNGKII
                     QELEAAFLGAGWNVIKVLWGTRWDPLLHRDTKGLLRRLMMECVDGEYQNYKAKGGAYT
                     REHFFGKYPELKEMVANMSDEEIWRLNRGGHDALKVYNAYRAATEHKDQPTVILAKTV
                     KGYGMGAAGEGQNITHQQKKLNDAALREFRDRFNIPVSDEEIAHVPYYRPSENSEELK
                     YLQERRTALGGYIPQRQSSAPPLQVPPLEAFKPLLEASGDREISTTMALVRLLGVLLK
                     DKNIGRHVVPIVPDEARTFGMEGMFRQVGIYSSMGQLYTPQDADQLLYYREDKKGQIL
                     EEGINEGGAMCSWIAAGTSYANQGVYMVPFYIYYSMFGFQRVGDFAWAAGDMRTRGFL
                     VGGTAGRTTLAGEGLQHQDGHS"
     gene            2134..3681
                     /locus_tag="VD737_01775"
     CDS             2134..3681
                     /locus_tag="VD737_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HXW09320.1"
                     /translation="MIATLPPPTAVRVTTTGPALTEQALAELDAVVLLLDRAPDVAQT
                     LATLPFGALWQALLAHERRAGNEWPILATRLPNGRQTLAVAGFVRTKSTGFERLDLAG
                     KLAHEVVKPGVARLQVHSVDGPAPRGDAHATLEAVLSAVLAHAAPMPELKSAPTPAAS
                     LAHVEVGSPDHASIERCVAEAKGNHLARWLATLPPNVLDSPSYRAALEQLATREGWGF
                     DFLDVAALEKLGAGAFLAVARANAHGGAGIARLSYRPPRPQANGAGIALVGKGICFDT
                     GGINLKPHKSMFTMHGDMQGSAVAVGTLLALSQLQVPYAVDCWLAITENQIGPTAFRP
                     QEVVRAANGVTIQVVHSDAEGRMVLADALTLASREKPRLVVDFATLTGACVVALGDRY
                     AGAFTNRAGLHDWLQRTGRDSGERVWCFPMDEDFDVELESKVADVQQCTPDSKGDHIL
                     AARFLSRFVEPDVPWVHLDLAASEHKGGLAHVPTEFTGFGVRYAVHLLGDAAALDERL
                     KALGNTD"
     gene            3678..4736
                     /gene="pyrC"
                     /locus_tag="VD737_01780"
     CDS             3678..4736
                     /gene="pyrC"
                     /locus_tag="VD737_01780"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HXW09321.1"
                     /translation="MKTAPDTVTLRRPDDWHIHFRDGAHLAAVVPHTARQFGRALAMP
                     NLKPPMTTTGALEAYRARILAAVPAGLAFEPMMTLYLTDATTPAEIERARDAGFVVGA
                     KLYPAGATTHSDAGVTSIDEVWPALEAMAEHGLVLQVHGEVADPGVDVFDREQAFIDR
                     VLSRVVDRVPGLKVVFEHITTRGAAQFVRAARKGVAATITPQHLLLNRNAIFDGGLRP
                     HHYCLPVLKRELDREALIVAATSDSPRFFLGTDSAPHARHTKEAPCGCAGIFSAHAAL
                     ELYAGAFDQAGRLDRLEAFASERGADFYGRPRNEGTVTLRRERWTVPESFDYGPGRDR
                     LVPFRAGDALEWRFEGTA"
     gene            4736..5422
                     /gene="rnt"
                     /locus_tag="VD737_01785"
     CDS             4736..5422
                     /gene="rnt"
                     /locus_tag="VD737_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HXW09322.1"
                     /translation="MDEFVFVEPRIVDRFRGFLPVVIDIESGGFNHQTDALLEIAAVM
                     IEMSPLGELRPGATHRYHVQPFHGSRLDPASLAVTGIDPHHPLRPAMPEKEALTQLFR
                     EVRKEVRERQCTRAVLVGHNASFDLNFLNAAIARTDIKRNPFHPFSSFDTATLGGVAF
                     GQTVLSRAIMAAGLDWDPAAAHSAIYDAEKTAALFCIICNRFRSLYEEARERAPQLAQ
                     REPIPPEESV"
     gene            complement(5509..5835)
                     /gene="grxD"
                     /locus_tag="VD737_01790"
     CDS             complement(5509..5835)
                     /gene="grxD"
                     /locus_tag="VD737_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002907768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HXW09323.1"
                     /translation="MTDVIQRIQEQLKSNPVVLYMKGTPDFPQCGFSASVVRTLEACG
                     AQFAYVNIFEDPELREALKRYSNWPTYPQLYVNGELLGGSDIINEMFQKGELQQVLAE
                     AGKASV"
     gene            complement(5916..6119)
                     /locus_tag="VD737_01795"
     CDS             complement(5916..6119)
                     /locus_tag="VD737_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_252441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HXW09324.1"
                     /translation="MTHRPASPCVNVCELDSSGTCVGCRRTIDEIARWGRMSAAEQWQ
                     VIARLEREPRGAPPACAAAAADA"
     gene            6254..6856
                     /locus_tag="VD737_01800"
     CDS             6254..6856
                     /locus_tag="VD737_01800"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HXW09325.1"
                     /translation="MNPLNSVRGAITTGFVLAIVLSIVIFPTTFHELGLARWLHIVTG
                     ITWIGLLYYFNLVQMPALTVAAADKGGPGGAGISKYVAPRALWWFRWAALATWLTGFW
                     YLARSGNLAGAFALGMLSEPINYYQLVIGIGAWLGTIMLFNVWVLIWPNQQKILGLKP
                     ASDEEKAQARKVAMLASRTNFVLSIPMLLCMGSATHGLPF"
     gene            7071..7925
                     /gene="nadC"
                     /locus_tag="VD737_01805"
     CDS             7071..7925
                     /gene="nadC"
                     /locus_tag="VD737_01805"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HXW09326.1"
                     /translation="MPTDLARTVAAALDEDVGGGDLTAALVPADRSGRATVITREAAV
                     VAGRPYVDEVFRQIDPRVSVEWLVADGDQVAPAQALYRLSGPARALLTGERSALNFYQ
                     VLSATATATRQYVDALAGSPSCKVLDTRKTLPGLRNAQKYAVRCGGGVNHRFGLYDGI
                     LVKENHIIAAGSIEAAVTAAKRQVSGRDGVLVEVEVETLDELREALDAGADMALLDEF
                     SLDDLRAAVKLSRSHRNGPMPLEASGGVTLETVAAIGATGVDFVSVGSLTKNVRAIDL
                     SMRFEYAA"
     gene            complement(7951..10653)
                     /locus_tag="VD737_01810"
     CDS             complement(7951..10653)
                     /locus_tag="VD737_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXW09327.1"
                     /translation="MSSEVPPSGGDDVQPQEPADFLSTQFVFGTGAPHPSVGLDVLVT
                     KVAVDFVGLTSEAGDSCVKRNLEALREATGVDAICIALLDPERKQVERVASATGLLAP
                     FDPQVLKAEPAERLPALAGRLEHLRLAELRDTRAPRRDHAVDAARLAELGIRSALVCG
                     LSLNNRVCGFMALFSGQPKADGWAVDLHLMLKLVGSSFATGLERLRVQRYLTKLEERN
                     SLSLPSANDGLWDFDVENNTVWFSPRWKQMLGYDDNDPNVSPDWRRLVHPDDMARVQA
                     LIRDHIAGKTQMFESVHRMRHVKGEWRWVVSRAKARVDATGRLRRLVGVEFDITERKL
                     YEDALFKEKESAQITLQSIGDGVITTDGHLVVEYLNPTAEELTGWKFEEAQGRNIDDI
                     FRGFHEETCEPLENPLSVAIRRARAIKSVRPTLLIKRDGNEMYIESTASPIRDGTGAV
                     CGGVLVFHDVSESRELNRKLSYHASHDILTGLVNRREFEQRLERALRSAKARETAYAL
                     CHLDVDQFKIINDTCGHSAGDALLGQVGTLLKTKIRWRDTLARLGGDEFGVLLESCSL
                     EEALRMAEQLKDTIRGYKFVWEERTFRLGCSIGVVPITGDSEDVAALLSAADSACAAA
                     KEAGRNRVFSFQENDIDLMRRRKEMQSAARVSNALEESRFALFRMPIKPLQKHDPGAH
                     YELLVRMIDEKGAIVAPSEFIGAAERYGIAPQIDRWVVMNALRWLVSEADERERLALC
                     SINLSGQSITDEKFLPFVQDLLKTSGINGSKICFEITETSAIQSYSQANRFMSALKQE
                     YGCMFALDDFGTGLSSFQYLKHFPVDFLKIDGSFVREILHDPIDREMVRSINEIGHLT
                     NKLTIAEFAENQEIINELRDLGVDYAQGYGVASPQRILKIAANG"
     gene            complement(10797..>11235)
                     /locus_tag="VD737_01815"
     CDS             complement(10797..>11235)
                     /locus_tag="VD737_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09328.1"
                     /translation="VLVTVGFVVYPQIDAVRSGRAFMQRVADASRDLEELALVGSKEQ
                     YLLHLPRPSVNFGHARWREKEQEAADAAAGFAARSGRAVLVDGKTRRLCFANAQASEV
                     GRANRQTWYLVTAGVADAGCIERGDLRRARLYIPSIASTNSAG"
BASE COUNT         1758 a         3875 c         3739 g         1863 t
ORIGIN      
        1 tggctgtgcc cgtcctggtg ctgcaggcct tcgcccgcaa gcgtggtgcg gccggccgtg
       61 ccgccgacca ggaagccgcg cgtgcgcatg tcgccggcgg cccaggcgaa gtcgccgacg
      121 cgctggaagc cgaacatcga atagtagatg tagaacggca ccatgtagac gccctggttg
      181 gcgtaggagg tgcccgcggc gatccagctg cacatcgcgc cgccttcgtt gatgccctcc
      241 tcgaggatct gtcccttctt gtcctcgcgg taatagagca gctggtccgc gtcctgcggc
      301 gtgtagagct ggcccatcga cgagtagatg cccacctgcc ggaacatgcc ctccatgccg
      361 aacgtgcggg cctcgtcggg cacgatcggc acgacgtgcc ggccgatgtt cttgtccttg
      421 agcaggactc cgagcagccg caccagcgcc atggtcgtcg agatctcgcg gtcgcccgac
      481 gcttcgagca gcggcttgaa cgcctcgagc ggcggcacct ggagcggtgg cgcgctcgac
      541 tggcgctgcg ggatgtagcc gcccagcgcg gtccggcgct cctgcaggta cttgagctcc
      601 tcgctgttct ccgacggccg gtagtacggc acgtgcgcga tctcctcgtc cgagaccggg
      661 atgttgaagc ggtcgcggaa ctcacgcagc gcggcgtcgt tgagcttctt ctgctggtgc
      721 gtgatgttct gcccttcgcc ggccgcgccc atcccgtagc ccttgacggt cttggcgagg
      781 atcacggtcg gctggtcctt gtgctccgtc gcggcgcggt acgcgttgta gaccttgagc
      841 gcgtcgtggc cgccgcggtt gagtcgccag atctcctcgt cggacatgtt ggcgaccatc
      901 tccttgagct cgggatactt gccgaagaaa tgctcgcgcg tgtacgcgcc gcccttcgcc
      961 ttgtagttct ggtactcgcc gtcgacgcac tccatcatca ggcgccgcag caggcccttg
     1021 gtgtcacggt gcagcagcgg gtcccagcgc gtgccccaca gcaccttgat cacgttccag
     1081 ccggcgccga ggaacgcggc ctcgagttcc tggatgatct tgccgttgcc gcgcaccgga
     1141 ccgtcgagac gctggaggtt gcaattgatc acgaacacaa ggttgtcgag tttctcgcgc
     1201 accggcatgg tgatcgcgcc gagcgactcg ggctcgtcca tctcgccgtc gcccaggaac
     1261 gtccagacct tgcggtcgct cggcggcacg atgccgcggt gttcgaggta acggatgaag
     1321 cgcgcctggt agatcgccat catcgggccg aggcccatcg acaccgtcgg aaactgccag
     1381 aactccggca tcagccaagg gtgcgggtac gaggagagcc cgccgccgcc gacttcctca
     1441 cggaacaggc gcaggtgatc ctctgtgagc cggccttcga ggtaagcgcg agcatagatg
     1501 cccggcgccg agtggccctg cacgaacacc atgtcgccag ggtgccggtc gctcggcgcc
     1561 cgccagaaat ggttgaagcc gacttcatac agcgtcgccg ccgaggcgta cgacgcgatg
     1621 tgcccgccga actccgagct ctgccggttg gcctgcacga ccatcgccat cgcgttccag
     1681 cgcaggtacg cctcgatccg ccgctccagc ccgcgatctc cgggataggg ctgttcctgg
     1741 gcgcgcgaga tcgtgttgac gtaagccgtg ttcggcttga acggcaggta ggcgccggac
     1801 cggcgcgtga agtcgatcac gcgttcgagc aggtactgcg cgcgctcggc accgtgcgtc
     1861 ttcagcaccg aatcgatgga ctcgagccac tcgcgcgtct cgtccgggtc agcgtcctcg
     1921 atcttcgaag cttcgaacat tccctggtct ccggcccgct tcgcacgaac gcatccatgc
     1981 gatgccgggc gtggtgaatt gccgagcacc cctgctagca tgccgccccg cgcaccggcg
     2041 cgcccggccg cagacggcgg cagcaggccc gccatgatcc ggtccgggcc gggtcaaggc
     2101 aagcgataag aacctccact ccaagaagct cgaatgattg caacacttcc accgccgacc
     2161 gcggtccgtg tcacgacgac cggtccagcc ctcacggaac aggcgctcgc cgagttggac
     2221 gcagtcgtgc tgctgctgga ccgcgcgccg gacgttgcgc agacgctcgc gacgttgccg
     2281 ttcggagcgc tgtggcaggc gctgctcgcg cacgagcgtc gcgccggcaa cgagtggccg
     2341 atcctcgcca cgcggctgcc gaacggacgg cagacgctgg cggtcgcagg gttcgtccgg
     2401 acgaagtcga ccggattcga gcgtctcgac cttgccggaa agctcgctca cgaggtggtc
     2461 aagccgggtg tcgcgaggct gcaggtgcac tcggtcgacg gccccgctcc acgcggcgac
     2521 gcgcacgcga cgctcgaagc cgtgctgagc gcagtcctgg cgcacgcggc gccgatgccg
     2581 gaactcaaga gcgcgcccac gccggcagcc tcgctcgcgc acgtcgaggt gggctcgccg
     2641 gaccacgcgt ccatcgagcg ctgcgtcgcg gaagcaaagg gcaaccacct ggcccgctgg
     2701 ctcgcaacgc tgccgccgaa cgtgctcgac tcgcccagct atcgggcggc gctggagcag
     2761 ctcgcgacgc gcgaaggctg gggattcgac ttcctcgacg tcgccgcgct cgagaagctc
     2821 ggggcgggcg ccttcctggc cgtcgcgcgc gccaacgcgc acggcggcgc gggcatcgcg
     2881 cgcctgagct accgtccgcc acgaccgcag gcgaacggcg ccggcatcgc gctggtcggc
     2941 aagggcatct gcttcgacac tggcggcatc aacctgaagc cgcacaagag catgttcacg
     3001 atgcacggtg acatgcaggg cagcgcggtc gcggtcggta cgctgctcgc gctctcgcag
     3061 ctgcaggtgc cgtatgccgt cgactgctgg ctcgcgatca ccgagaacca gatcgggccg
     3121 acggcgtttc gcccgcagga ggtcgtgcgc gccgcaaacg gcgtgacgat ccaggtcgtg
     3181 cacagcgacg cggagggacg catggtgctc gccgacgcgc tgaccctcgc gtcgcgtgag
     3241 aagccccgcc tcgtcgtcga cttcgcgacg ctgaccggcg cctgcgtcgt cgccctcggc
     3301 gaccgttacg cgggcgcgtt caccaatcgt gccgggctcc acgactggct gcagcgcacc
     3361 ggccgcgaca gcggcgagcg cgtctggtgc ttcccgatgg acgaggactt cgacgtcgaa
     3421 ctcgagagca aggtcgccga cgtccagcag tgcacgccgg acagcaaggg cgaccacatc
     3481 ctcgcggcgc gattcctgag ccggttcgtc gagccggacg tgccctgggt gcacctcgac
     3541 cttgccgcgt cggagcacaa gggcggcctc gcgcacgtcc ccaccgaatt cacgggcttc
     3601 ggggtacgct atgcagtcca cctgctcggc gacgcggccg ccctcgacga gcggctgaag
     3661 gcccttggga acaccgattg aagaccgccc ccgacacagt caccctgcgc cgccccgacg
     3721 actggcacat ccacttccgc gacggtgcgc accttgcggc ggtggtgccg catacggcac
     3781 ggcagttcgg gcgcgcgctc gcgatgccga acttgaagcc gcccatgacg acgacggggg
     3841 cgctcgaggc gtatcgcgcg cgcatcctgg cggccgtgcc ggcggggctc gcgttcgagc
     3901 cgatgatgac gctgtacctg accgacgcga ctacgcccgc cgaaatcgag cgcgcgcggg
     3961 acgccgggtt cgtcgtcggc gccaagctct atccggccgg cgcgaccacc cacagcgacg
     4021 ccggcgtgac gtccatcgac gaagtgtggc ccgcgctcga agcgatggcc gagcacggac
     4081 tggtgctgca ggtgcacggc gaggtcgcgg atcccggcgt cgacgtgttc gaccgcgagc
     4141 aggccttcat cgaccgcgtg ctgagccgcg tcgtcgaccg cgtgcccggc ctgaaggtcg
     4201 tgttcgagca catcaccacg cgcggcgcgg cgcagttcgt gcgcgcggcg cgcaagggcg
     4261 tcgcggccac gatcacgccg cagcacctgc tgctcaaccg caacgcgatc ttcgacggtg
     4321 gcctccggcc gcaccattac tgcctgccgg tgctcaagcg tgagctggac cgcgaggcgc
     4381 tgatcgtggc ggcgacgagc gacagcccgc ggttcttcct gggcaccgac agcgctccgc
     4441 acgcacgcca cacgaaggaa gcgccgtgcg gatgtgccgg catcttctcg gcgcacgccg
     4501 cgctcgagct gtatgccggg gcgttcgacc aggccggccg tctcgaccgg ctcgaagctt
     4561 tcgcatccga gcgcggcgcc gacttctatg gccggccccg caacgaagga acggtcacgc
     4621 tgcgtcgcga acgctggacc gttccggaga gcttcgacta cggcccgggc cgcgaccgtc
     4681 tcgtgccctt ccgcgccggc gacgccctcg aatggcgctt cgaaggcacg gcctgatgga
     4741 cgagttcgtc ttcgtggagc cacgcatcgt cgaccgtttc cggggcttcc tgccggtggt
     4801 gatcgacatc gagtccggcg gtttcaacca ccagaccgac gcgctgctcg agatcgccgc
     4861 cgtgatgatc gagatgtcgc cgctgggcga gctgcggccc ggggcgacgc accgttacca
     4921 cgtgcagccg ttccacggct cgcgcctcga tcccgcgtcc ctggcagtca ccggcatcga
     4981 tccgcaccat ccgctgcgcc cggccatgcc ggagaaagag gctcttacgc agctgttccg
     5041 cgaagtgcgc aaggaagtgc gcgaacgcca gtgcacgcgc gccgtgctgg tgggtcacaa
     5101 cgcgtcgttc gacctcaact tcctgaacgc ggcgatcgcc cgcacggaca tcaagcgcaa
     5161 cccgttccac ccgttctcga gcttcgacac cgccacgctc ggcggcgtcg ctttcgggca
     5221 gaccgtgctt tcgcgggcga tcatggcggc gggcctggac tgggaccccg cggcggcgca
     5281 ctcggcgatc tacgacgcgg agaagacggc ggcgctgttc tgcatcatct gcaaccggtt
     5341 ccgcagcctc tacgaggagg cccgcgaacg tgcgccgcag ctcgctcagc gggagccgat
     5401 cccgccggaa gaaagtgtct agtgtctggt gtctagcggt tagtaggaac gaagttggag
     5461 gcgccggagc aagccgctgc cctcttacta gacactagac actaaccgct aaacactcgc
     5521 cttgcccgcc tccgccagca cctgctgcag ctcgcccttc tggaacatct cgttgatgat
     5581 gtccgaaccg ccaagcagtt cgccattgac gtacagctgc gggtacgtcg gccagttcga
     5641 atagcgcttg agcgcctcac gcagctccgg gtcctcgaag atgttgacgt aggcgaattg
     5701 cgcgccacac gcctcgagcg tgcgcaccac cgacgccgag aagccgcact gcgggaagtc
     5761 cggcgtgccc ttcatgtaga gcaccacggg attcgacttg agctgttcct ggatacgctg
     5821 gatgacgtcc gtcacgatcg acccctcggc cactctgtgg ccagacattg catacgattt
     5881 cgaaaagcca agactgaacc cgcgtgcctg ccgacctacg cgtcggccgc ggcagcagcg
     5941 cacgccggcg gcgctccgcg tggctcgcgc tcgagacggg caatgacctg ccactgctcg
     6001 gcggcgctca tccggcccca gcgcgcgatc tcgtcgatcg tgcggcgaca tccgacgcac
     6061 gtgccgctcg aatcgagctc gcagacgttt acgcagggcg atgcgggacg gtgggtcatg
     6121 cggcctcgtg cgcggggcgc gcaggacgtt gaattatgcg ggtccgtccc ctattcttca
     6181 aatccaacct gcggccgggc tcgccataaa cccccgccag ccaaagaatt ttcacccggg
     6241 aggcttccct gacatgaacc cgctgaattc ggtgcgcgga gccatcacga ccggcttcgt
     6301 gctggccatc gtgctgtcga tcgtgatctt cccgaccacg ttccacgagc tcggcctcgc
     6361 ccgctggctg catatcgtca cgggcatcac ctggatcgga ctgctgtact acttcaacct
     6421 cgtacagatg cccgccctga cggtggccgc cgccgacaag ggggggccgg gcggcgccgg
     6481 aatcagcaag tacgtcgccc cgcgtgcgtt gtggtggttt cgctgggccg cgctggcgac
     6541 atggctcacg gggttctggt acctcgcccg gtcgggcaac ctggccggcg cgttcgcgct
     6601 gggcatgctc agcgaaccga tcaattacta ccagctcgtg atcggcatcg gcgcatggct
     6661 cggcaccatc atgctgttca acgtctgggt gctgatctgg ccgaaccagc agaagatcct
     6721 cggcctcaag ccggcgtccg acgaggagaa ggcccaggcg cgcaaggtcg cgatgctcgc
     6781 gtcgcggacg aatttcgtgc tgtcgatccc gatgctgctc tgcatgggct cggcgaccca
     6841 cggcctgccg ttctgacgga ccgggcgagc ctcggccccg cccgtccgcg atggaacagt
     6901 gaaccaggac ccggcagccg ccgggtcttt ttttgtcttt cctgtacgtc ccgacggaca
     6961 atcgacagat ggccacgacc cgaagatcga ccggcacgac gaatgtttcg ggcggcggcg
     7021 tgcgacgaaa tcggggcgcc cgttcccacg acccaggtgc ggcgccgcag gtgcccaccg
     7081 acctcgcgcg tacggtcgca gcggcgctcg acgaggatgt cggcggcggc gacctgacgg
     7141 cggcgctggt gccggcggac cgtagtggtc gcgcgacggt gatcacgcgc gaagccgccg
     7201 tcgtggccgg ccggccgtac gtcgacgagg tgttccgcca gatcgacccg cgcgtgagcg
     7261 tggaatggct ggtggcggac ggcgaccagg tggcgccggc gcaggcgctg tatcgcctga
     7321 gcggtccggc gcgtgcgctg ctcaccggcg agcgaagcgc gctcaatttc taccaggtgc
     7381 tctcggccac cgcgaccgcg acgcggcagt acgtcgacgc gctcgccggc tcaccgtcgt
     7441 gcaaggtgct cgacacgcgc aagaccttgc caggcctgcg caacgcacaa aagtacgcgg
     7501 tgcgctgcgg cggcggcgtc aaccaccgtt tcgggctcta cgacggcatc ctggtcaagg
     7561 aaaaccacat catcgcggcg ggctcgatcg aagcagccgt cacggccgcg aaacgacagg
     7621 tgtccggccg cgacggcgtg ctcgtcgagg tggaggtcga gacgctcgac gaactgcgcg
     7681 aggcgctcga tgcgggggcc gacatggcac tgctcgacga gttctcactc gacgacctgc
     7741 gggcggcggt caagctcagc cgctcgcacc gcaacggccc gatgccgctc gaggcgtcgg
     7801 gcggcgtcac gctcgagacc gtggcggcga tcggggcgac gggcgtcgat ttcgtctcgg
     7861 tcggctcgct caccaagaac gtgcgggcga tcgacctgtc gatgcggttc gaatacgcag
     7921 cgtagcccgg agagcgtcca ggcagagacc tcagccgttc gcggcgatct tgaggatccg
     7981 ctgcggcgag gcgacgccgt agccctgcgc gtagtcgacg ccgagatccc gcagctcgtt
     8041 gatgatctcc tggttctcgg cgaactcggc gatcgtgagc ttgttggtga ggtgcccgat
     8101 ctcgttgatc gacctcacca tctcgcggtc gatcgggtcg tggaggatct ccctgacgaa
     8161 gctgccgtcg atcttgagga agtcgaccgg gaagtgcttc agatactgga acgacgagag
     8221 gcccgtcccg aagtcgtcga gcgcgaacat gcagccgtac tcctgcttga gcgcgctcat
     8281 gaaccggttg gcctgcgagt agctctggat cgccgacgtc tccgtgatct cgaagcagat
     8341 cttcgagccg ttgatgccgc tggtcttcag gagatcctgc acgaacggca ggaatttctc
     8401 gtcggtgatg ctctgccccg agaggttgat cgagcacagt gccagccgct cgcgttcgtc
     8461 ggcctcggac accagccagc gcagcgcatt catgacgacc cagcggtcga tctgcggcgc
     8521 tatcccgtag cgttccgccg cgccgatgaa ttcgcttggc gcaacgatgg ctcccttctc
     8581 gtcgatcatc cgcacgagca attcgtagtg cgcgcccgga tcatgcttct gcagcggctt
     8641 gatcggcatg cggaagagcg cgaagcgcga ctcctcgagc gcgttgctca cgcgtgccgc
     8701 ggactgcatc tccttgcggc ggcgcatgag gtcgatgtcg ttctcctgga agctgaacac
     8761 gcggttgcgt cccgcctcct tcgctgcggc gcaggcgctg tcggccgccg agagcagggc
     8821 cgcgacatcc tcgctgtcgc ccgtgatcgg cacgacgccg atgctgcagc cgagccggaa
     8881 cgtgcgctcc tcccagacga acttgtagcc ccggatcgtg tccttgagct gctccgccat
     8941 gcgcagcgct tcttcgagcg aacagctctc gagcagcacg ccgaactcgt ccccgccgag
     9001 acgcgcgagc gtgtcgcgcc agcggatctt ggtcttgagc agcgtgccca cctggccgag
     9061 cagcgcgtcg ccggcactgt ggccgcaggt gtcgttgatg atcttgaact ggtccacgtc
     9121 gaggtggcac agcgcatacg cggtctcgcg cgccttggcg ctgcgcagcg cgcgctcgag
     9181 ccgctgctcg aactcgcgcc ggttgaccag tcccgtgagg atgtcgtggc tcgcgtggta
     9241 cgagagcttg cggttgagtt cgcgactctc gctcacgtcg tggaacacga gcacgccgcc
     9301 gcagaccgct ccagtgccgt cgcggatcgg cgacgcggtg ctctcgatgt acatttcgtt
     9361 gccgtcgcgc ttgatcagca gcgtcggacg caccgacttg atcgcgcggg cacgacggat
     9421 cgcgaccgac agcgggttct cgagcggctc gcaggtctcc tcgtggaacc cgcggaagat
     9481 gtcgtcgatg ttgcggccct gcgcttcctc gaacttccag ccggtcagtt cctcggccgt
     9541 cgggttcagg tactcgacga cgaggtgccc atcggtggtg attacgccgt cgccgatcga
     9601 ctgcaacgtg atctgcgcgc tctccttctc cttgaacagc gcgtcctcgt agagcttgcg
     9661 ctcggtgatg tcgaactcga cgccgaccag gcgtcgcagc cgacccgtcg cgtccacgcg
     9721 cgccttggcg cggctcacga cccaccgcca ctcgcccttg acgtggcgca tgcggtgcac
     9781 gctctcgaac atctgggtct tgccggcgat gtggtcgcgg atcagtgcct gcacgcgcgc
     9841 catgtcgtcc gggtgcacga gccgccgcca gtctggcgag acgttcgggt cgttgtcgtc
     9901 gtagccgagc atctgcttcc agcgcggcga gaaccagacc gtgttgttct cgacgtcgaa
     9961 atcccacagt ccgtcgttgg cggaaggcag cgagagcgag ttgcgttcct cgagcttggt
    10021 caggtaacgc tgtacgcgca gccgttcgag gccggtggcg aagctcgagc cgaccagctt
    10081 cagcatcagg tgcaggtcga ccgcccagcc gtcggccttc ggctgcccgg agaaaagcgc
    10141 catgaagccg caaacgcggt tgttgagcga cagcccgcag acgagtgcgc tgcggatgcc
    10201 gagctcggcc aggcgggcag cgtcgacggc gtggtcgcgg cgcggcgccc gcgtgtcgcg
    10261 cagttccgca agacgcaggt gctcgagccg gccggccagg gctggcaggc gctctgcagg
    10321 ttcggccttc aacacctggg ggtcgaatgg cgccagcagc ccggtggcgc tggccacacg
    10381 ctcgacctgc ttgcgctcgg gatcgagcag cgcgatgcag atcgcgtcga cgccggtcgc
    10441 ctcgcgcagc gcctcgaggt tgcgcttgac gcagctgtcg cccgcctcgc tcgtcagccc
    10501 gacgaagtcg accgccacct tggtgacgag aacatcgagg ccgacggacg gatgcggtgc
    10561 gccggtgccg aagacgaatt gggtggacag gaagtcggcc ggttcctgcg gctgtacgtc
    10621 atcgccaccg ctcggcggga cttcggagga catcgcgctt tccggtctcc ctgtttccgg
    10681 gcgctgcacg tgacgcagcg ttacccgacg cacgccgctc cgcctggatt tgacataggt
    10741 cggcggaccg ggccgtacgc gcgagggatt ctgacagaaa gagtcgggac ttcaagctaa
    10801 ccagctgaat ttgttgacgc aatcgacgga atgtacaaac gcgcacgacg caggtcgccg
    10861 cgctcgatac agcctgcgtc ggcgactcct gccgtcacga ggtaccacgt ctggcgattt
    10921 gcgcgaccga cctcgctggc ttgtgcgttc gcgaagcaca accttcgcgt cttgccgtcc
    10981 accagcactg cccggccaga ccgcgccgca aaccccgcag ccgcgtcggc cgcctcctgt
    11041 tctttctcgc gccagcgcgc gtggccgaaa ttgacgctcg gtcgaggcag gtgcagcagg
    11101 tactgctcct tcgagcccac tagggcgagt tcctcgaggt cgcgcgacgc atccgccacg
    11161 cgctgcatga acgcccgccc cgaacgcacg gcgtcgatct gcggatacac gacgaacccc
    11221 acggtgacga gcacg
//
LOCUS       DASPFR010000075         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1243740, whole genome shotgun sequence.
ACCESSION   DASPFR010000075 DASPFR010000000
VERSION     DASPFR010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243740"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="VD737_01820"
     CDS             <1..1321
                     /locus_tag="VD737_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXW09329.1"
                     /translation="YHKRVLVNAGLWGGAEAQLDRLWNEVDLGSPGAGSPALPFDCVL
                     TAEERTFRDEARTFLAQELDDDLRVATRRLTCTFLDRDVTDRWHARLNRRGWLAPLWP
                     REHGGTGWSAVERFVFEYEAAIAGAPERIPMGLRYVGPIVAQFGSAWQKEYFLPRILS
                     GEHYWAQGFSEPQAGSDLAAVKTSAVLDGDHYVVNGTKIWTTNAHLADWVFCLVRTDA
                     SGKAQEGISFLLIDLRSPGIRVEPISLLAVDHEVNQVFFDEVRVPREHLVGEPGRGWQ
                     YAKFLLELERGGTVLCGRVRAEFEAVKEHIAAARPALRKDPAVRRRLAELEVRLMALE
                     AYELRTARAMTNATEPGVGGSVVKLVSSELQQDITQFGVEIAGPAALEFEPTRPLPAP
                     ESLGYPGHELDLVAMPRYLNTRATTIFAGSSEIQREIIAKHVLGFRA"
     gene            1336..>2085
                     /locus_tag="VD737_01825"
     CDS             1336..>2085
                     /locus_tag="VD737_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HXW09330.1"
                     /translation="MDDSNETTASGPRRKYLGDVGAVHEGMELEERRLAAYLAGAVKG
                     FRGPVRVQQFDGGQSNPTYLLHTAAERFVLRRRPPGVLLESAHQVHREFRVMSALHGV
                     GFPVPEPVAMCFDEDVIGSQFYVMRFVPGRIFCDCRMPDLASDERARVFDSVNETLAR
                     LHVLDPVRLGLGDFGRPGNYFARQTARWSRQYEGSRTQDIPEMERLMAWLPGAVPSDD
                     GRVRVIHGDFSFHNLLIDPATGKVAAVIDWEL"
BASE COUNT          324 a          691 c          739 g          331 t
ORIGIN      
        1 ctatcacaag cgcgtgctcg tcaacgcggg tttgtggggt ggtgcggagg cgcagctcga
       61 ccggctctgg aacgaagtcg acctcggcag ccccggcgca ggcagcccgg cactgccgtt
      121 cgactgcgtg ttgaccgccg aggagcgcac gttccgcgac gaggcacgaa cgttcctggc
      181 gcaggagctc gatgacgacc tccgcgtcgc gacacgacgg ctcacctgca ccttcctcga
      241 ccgcgacgtg accgaccgct ggcacgccag attgaaccgt cgcggctggc tcgcgcccct
      301 gtggccgcgc gaacacggcg gcacgggctg gagcgccgtc gagcgcttcg tgttcgagta
      361 cgaagcggcg atcgccggcg cgcccgagcg gatcccgatg gggctgcgat acgtcggtcc
      421 catcgtggcc cagttcggct cggcgtggca gaaggaatac ttcctgccca ggatcctgtc
      481 cggagagcac tactgggccc agggtttttc cgagccgcag gccgggtcgg acctcgctgc
      541 cgtgaagacc agcgcggtgc tggacggcga tcactacgtc gtcaacggca ccaagatctg
      601 gacgacgaac gcgcatctgg cggactgggt cttctgcctc gtgcggaccg acgcgagcgg
      661 gaaggcccag gaaggcatca gcttcctgct gatcgacctg cgctcgcccg gcatccgggt
      721 cgagcccatc tccctgctgg ccgtcgacca cgaggtcaac caagttttct tcgacgaggt
      781 ccgcgtgccg cgcgagcacc tggtgggcga gcccgggcgc ggctggcagt acgcgaagtt
      841 cctgctcgag ctcgagcgcg gcggcacggt gctctgcggt cgagtgcgag cggagttcga
      901 agcggtcaag gaacacattg ccgcggcacg tcccgcgctg cggaaagatc ccgcggtacg
      961 tcgtcgcctg gccgagctcg aggtccgcct gatggcgctc gaggcctacg agttgcgcac
     1021 ggcccgcgcg atgaccaacg ccacggagcc gggcgtaggc ggctcggtcg tcaagctggt
     1081 gtcctcggaa ctgcagcagg acatcacgca gttcggcgtg gagatcgccg gacctgcagc
     1141 gctggagttc gaaccgacgc ggccgctgcc ggcgccggag tcactcggct atccgggtca
     1201 cgaactcgac ctcgtggcga tgccgcgcta cctgaacacg cgcgcgacga cgatcttcgc
     1261 gggctcgagc gagatccagc gggagatcat cgccaagcac gtcctgggat tccgcgcgtg
     1321 atggacgggg aagcgatgga cgacagcaac gaaacgacgg cgtccggacc gcggcgcaag
     1381 tacctgggcg acgtcggtgc cgtgcacgag ggcatggagc tcgaggaacg ccgcttggcc
     1441 gcatacctgg ccggcgcggt gaagggcttc cgcggtcccg tgcgggtgca gcagttcgac
     1501 ggtggccagt cgaacccgac gtacctgctg cacaccgcgg cagagaggtt cgtgctgcga
     1561 cgtcgtccgc ccggcgtgct gctcgaatcg gcgcaccagg tgcaccgcga gttccgcgtg
     1621 atgtcggccc tgcacggcgt gggctttccc gtgcccgagc ccgtggccat gtgtttcgac
     1681 gaggacgtga tcggctcgca gttctacgtg atgcgtttcg tgccggggcg catattctgc
     1741 gactgtcgca tgccggatct cgcgtcggac gaacgcgccc gggtattcga ctccgtcaac
     1801 gagacgctgg ctagactgca cgtcctcgac ccggttcggc tcgggctcgg tgacttcggc
     1861 cggcccggca actatttcgc gcgtcagacg gcgcggtgga gccggcagta cgagggctcc
     1921 cgcacgcagg acataccgga gatggagcgc ctgatggcct ggctgcccgg cgcggtgccg
     1981 tcggacgacg ggcgcgtccg cgtcatccac ggcgactttt cgttccacaa cctgctgatc
     2041 gatccggcga ccgggaaggt ggcggccgtg atcgactggg agctc
//
LOCUS       DASPFR010000076         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1610677, whole genome shotgun sequence.
ACCESSION   DASPFR010000076 DASPFR010000000
VERSION     DASPFR010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610677"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="VD737_01830"
     CDS             complement(<1..1120)
                     /locus_tag="VD737_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HXW09331.1"
                     /translation="MSMKWEGKGFMESLPRIYAIYTGGFLAFFALMAVLEQFGLSARW
                     IGILFVLFTLVVYAFIGVLSRTTAVEAYYVAGRSVPPVFNGMATAADWMSGASFVAMA
                     GGVFMSGHPYMAFIVGWTGGYILIATLMAPYLRKFGCYTVPDFIGTRYGGRATRLAAI
                     VVLIVASFTYVTAQITATGTIAARAFQIPFEIGVWLGLLGILVCSMLGGMRAVTWTQV
                     AQYIVLIIAYLIPIFWMSISQGFGFLPHLSYGSAVQRIMELEPVIGVGTAAVDSVEGL
                     KVLTALHATPPEGALAAWQFVTLVFCMMVGTASLPHILMRYFTTPSVRDARTSVAWSL
                     FFIFLLYFSAPALATFSKLQMIDPNLATSIIGASIDQVM"
     gene            complement(1133..1399)
                     /locus_tag="VD737_01835"
     CDS             complement(1133..1399)
                     /locus_tag="VD737_01835"
                     /inference="COORDINATES: protein motif:HMM:NF025305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HXW09332.1"
                     /translation="MRQLSPEDRAEHWRRTRGLMLAHLSVWFIFAYVVHWFARDLNAI
                     NFFGWPLGYYMSAQGSLIVFVVQLFLFSRQQHAIDVKFGVEEQE"
     gene            complement(1668..>3088)
                     /locus_tag="VD737_01840"
     CDS             complement(1668..>3088)
                     /locus_tag="VD737_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09333.1"
                     /translation="SIDVSYSVASRSAVSLAGSAVSVTGNMLEAYAPVTATATVNVTR
                     PPAGGITFALSGTSSSVQSVAALSQGPGTADLQLTFHAAASVGIGTHTEDVRVRACYD
                     PACLREIPGSPLVITTSYVVVSGIRPEEGVAPLAYLSRKLLSHDVVDAEYARAHDAIA
                     MVSSAPTHAFYLYDVTTGTERQVTLNRAPTAVSVSPDGQSVAVGHDALITHIDLAALV
                     AGTASTKLLDVSARVGDLVLDGHGKVHALPAADQWVDVHSVDVATNTETRKYGLLYAG
                     SRGRLHPSGDYMYTADNGLSPSDIAKYDLRSGVATRLYDSPYHGDYAMCGNVWFKEDG
                     ATIYTRCGNTFRASTLQSQDMVYGGRLQLSTSQFYGYQIDALSQADSSKEIVLLETDS
                     YECAPFRSNDRCYSHLAVYESDFLNRTGLYSLAPIDVAGNTYMQRGLFVFHSRDGLHR
                     YLISRLDPAPNATQSHYLTVVQ"
BASE COUNT          566 a          994 c         1043 g          485 t
ORIGIN      
        1 ccatcacctg gtcgatcgag gcgccgatga tcgaggtcgc gagattcggg tcgatcatct
       61 gcagcttgct gaacgtcgcg agcgcaggcg ccgagaagta cagcaggaag atgaagaaca
      121 gcgaccaggc cacggacgta cgggcatcgc gcaccgatgg cgtcgtgaag tagcgcatca
      181 ggatgtgcgg cagcgaggcc gtgccgacca tcatgcagaa gaccagggtg acgaactgcc
      241 acgccgcgag cgcgccctcg ggcggcgtcg cgtgcagcgc cgtgagcact ttgaggcctt
      301 cgacgctgtc gacagccgcc gtgccgacgc cgatgaccgg ctcgagctcc atgatccgct
      361 ggacggccga gccgtacgaa aggtgcggca ggaagccgaa gccctgcgag atcgacatcc
      421 agaagatcgg gatcaggtac gcgatgatca gcacgatgta ctgcgcgacc tgcgtccagg
      481 tgaccgcgcg catgccgccc agcatcgagc agaccaggat gccgagcagg ccgagccaga
      541 cgccgatctc gaacgggatc tggaacgccc gcgcggcgat cgtgcccgtg gccgtgatct
      601 gcgcggtcac gtacgtgaac gacgcgacga tcagcaccac gatcgcggcg agtcgcgtcg
      661 cgcggccgcc gtagcgcgtg ccgatgaagt ccggcaccgt gtagcagccg aacttgcgca
      721 ggtagggcgc catcagcgtc gcgatcagga tgtagccgcc cgtccagccg acgatgaagg
      781 ccatgtacgg gtggccggac atgaacacgc cgcccgccat ggccacgaac gaggcgccgg
      841 acatccagtc cgcggcggtc gccatgccgt tgaacaccgg tggcacgctg cggcccgcga
      901 cgtagtaggc ctcgacggcc gtcgtgcgcg agaggacgcc gatgaacgcg tagacgacga
      961 gcgtgaacag cacgaacagg atgccgatcc agcgcgcgct gagaccgaac tgttcgagga
     1021 ccgccatcag cgcgaagaac gccaggaacc cgcccgtata gatcgcgtag atcctgggca
     1081 gggattccat gaaccccttg ccttcccatt tcatggacat gtgcgctccc ctttactctt
     1141 gttcttcgac gccgaacttc acgtcgatcg cgtgctgctg ccggctgaac aggaacagct
     1201 gcacgacgaa cacgatcagc gagccctggg cggacatgta gtagcccagt ggccagccga
     1261 agaagttgat cgcgttgaga tcgcgggcga accagtgaac gacgtaggcg aagatgaacc
     1321 agaccgacaa gtgcgcgagc atgagcccgc gcgtccggcg ccagtgctcg gcgcgatcct
     1381 cgggtgacaa ttgacgcacg acgaatcccc ctgtcgaagc caacaatcct gccctgtcta
     1441 ccaactggga tagcggttta ccactagcca ttggtcgaat gccgcttgcg gcaccccggg
     1501 ggcatcgtgc gtgcggacgt ctactcgggg ttcaggagcg cgtaccgggc cgcattcgac
     1561 caccgcgcct gcggccgcgg aacggtcgcc gcgcctcggc acctgtcgct cgcggtcgcg
     1621 ccgccttgct gccgcccggc tccggtcatt tcctggggcg gcgcgtctta ctgcaccaca
     1681 gtgaggtagt gactctgggt ggcgttgggt gccgggtcca gtcgactgat caggtagcgg
     1741 tgcagcccgt cgcgactgtg gaacacgaat aggcctcgct gcatgtacgt gttgcccgcg
     1801 acgtcgatcg gcgcgagcga atacaggccc gtgcggttca ggaagtcgct ttcgtagacc
     1861 gccaggtgtg aatagcaacg gtcgttcgaa cggaacggcg cgcactcgta cgagtcggtc
     1921 tcgagcagca cgatctcctt gctcgaatcg gcctggctca gcgcgtcgat ctggtagccg
     1981 taaaactggg acgtcgagag ttgtagccgg ccgccataga ccatgtcctg ggactgcagc
     2041 gtggatgccc ggaacgtgtt tccgcaccgg gtgtagatcg tcgcaccgtc ctccttgaac
     2101 cagacgttgc cgcacatcgc gtagtccccg tggtacggcg agtcgtaaag ccgggtcgcc
     2161 acaccgctgc gcaggtcgta cttcgcgatg tcactcggtg agaggccgtt gtcagccgtg
     2221 tacatgtagt cgcccgacgg gtgcaggcgg ccgcgtgacc cggcgtacag caggccgtat
     2281 ttcctcgtct cggtgttggt ggcgacatcc acggagtgaa cgtccaccca ctggtcggcc
     2341 gcaggcagcg catgcacctt gccgtgtccg tcgaggacca ggtcaccgac tcgcgcggag
     2401 acgtcgagca gtttcgtcga tgccgttccg gccacgagcg ccgccaggtc gatgtgggtg
     2461 atgagtgcgt cgtgccccac ggccacggac tgaccgtcgg gagacaccga gaccgccgta
     2521 ggggcccggt tgagcgtcac ctgacgctcc gtcccggtcg tcacatcgta gagatagaag
     2581 gcgtgcgtcg gcgcagacga aaccatggcg atggcgtcgt gggcgcgcgc gtattccgca
     2641 tccacgacgt cgtgggacag cagcttgcgc gacaggtacg ccagcggcgc cacgccctcc
     2701 tcgggccgga tgccactcac caccacgtag ctcgtggtga tcacgagcgg actgccgggg
     2761 atctcgcgca ggcaggccgg gtcgtagcag gcgcgcaccc gaacgtcctc ggtgtgcgtg
     2821 ccgatgccga ccgaggcggc agcgtggaac gtcaactgca agtctgccgt gcccgggccc
     2881 tgggaaagag ccgcgaccga ctgcacgctg gacgaagtgc cgctcagcgc gaaagtgatg
     2941 ccaccggccg gcggtcgcgt cacgttgacg gtggcggtcg ccgtcacggg cgcataggcc
     3001 tccagcatgt tgcccgtcac ggatacggcg gaccccgcga gtgaaacggc gctccggctg
     3061 gcgaccgagt agctgacgtc gatgctga
//
LOCUS       DASPFR010000077         6712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_23983, whole genome shotgun sequence.
ACCESSION   DASPFR010000077 DASPFR010000000
VERSION     DASPFR010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23983"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1762
                     /locus_tag="VD737_01845"
     CDS             <1..1762
                     /locus_tag="VD737_01845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXW09334.1"
                     /translation="GPGQLAADRAADRGLELAALAPEMLSALRGFLPPSTQHGNPVYV
                     RGDASARHFAEAARHVLADGATDALLAVMAPFVTTDPDEFADALIEVARAQKKPVFTC
                     WMGDAAVVESRQRFAENRIASYRTPEAAVDAIAALGLFTANQEQLLQVPEPLGVDSAP
                     DKARAQALLDEALAAGNEWLDPGESKELLAAFGIPVVRAVPAYSADEAVQVAEAAGFP
                     VAMKILSPDIAHKTDVGGVRLGRADARSVRDGYENMMRDVALARPDARLEGVLVEPMY
                     LEKYGRELMIGVVRDPVFGPAISFGLGGTMVEVIRDRAVALPPLNQFLAHDLVRRTRA
                     SLALQSLRGAPAAAREAVEDVLLRVSEIVCELPDVGAIDLNPVVVTAQGAVVVDARIG
                     VVRSPPGALPYRHMAIHPYPSDCRFTIELPDGERATVRPIRPEDARMEQEFVHRLSEE
                     SRFLRFMFGLHDLTPAMLSRFTQIDYDREMALIVVRHGTGGGDDEDRQIGVARYITLP
                     DEETCEFAIVVSDEWQGKGIARQLFGHLIDVARARRLKIMTGITLRENSRMIDLSRAL
                     GFQTKADVDEPELVRMTLAL"
     gene            complement(1789..2274)
                     /locus_tag="VD737_01850"
     CDS             complement(1789..2274)
                     /locus_tag="VD737_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09335.1"
                     /translation="MSSMLLAGTAAAQSPPVGMSDADDVLTSTEDWTAADVDEDGTLS
                     QSELNRAEPRLAAYFREIDVNGDWRISRDEIAAWQAGPEPGDIDADELPAGAVDAGGV
                     EQADEDRSATAPGRTDATRGERTDDLLPDDGDEATELLPDDADDDAADAGEVERDTGE
                     R"
     gene            complement(2934..3233)
                     /locus_tag="VD737_01855"
     CDS             complement(2934..3233)
                     /locus_tag="VD737_01855"
                     /inference="COORDINATES: protein motif:HMM:NF022786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3144 domain-containing protein"
                     /protein_id="HXW09336.1"
                     /translation="MDSETDEMFYRRADAHIDLANEQVADVSRGKVSASFMYAVARFN
                     SWVTACKADSAEDLRAGKEQSIEYFVSQYRAMLNENLDDYIENFDKYMRPDGNNA"
     gene            3435..3704
                     /locus_tag="VD737_01860"
     CDS             3435..3704
                     /locus_tag="VD737_01860"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HXW09337.1"
                     /translation="MEYEWDPAKAKANVRKHRISFADAALALEDPRALTVADPDAGGE
                     HRFVSLGSDPNGRVLVTVFTSRGSRTRIISSRKASPNERRIYESK"
     gene            3704..3958
                     /locus_tag="VD737_01865"
     CDS             3704..3958
                     /locus_tag="VD737_01865"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HXW09338.1"
                     /translation="MRKEYDFSKAKRGPAVAVPSGKTRITIRLDDDILEWFKTQVEAA
                     GGGNYQSLINAALREHMRRADEPLERTLRRVLREELKRAS"
     gene            complement(4057..4830)
                     /locus_tag="VD737_01870"
     CDS             complement(4057..4830)
                     /locus_tag="VD737_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXW09339.1"
                     /translation="MFDSFPHAGNVLVQGASRGIGLEFVRQCLAVPSVSHVVATCRNP
                     ASASGLDELRAHHAGRLTVTALDLLDDASIARAAKLVAEVVPRLHLLVNCAGVLHAAS
                     QGLKPEKRLADVDAGALAHSFAVNATGPLLVARHFEPLLAHPERAVFASLSAKVGSIS
                     DNRLGGWYAYRAAKAAQNQISRTLSIEWARSRRNVIVVSLHPGTTDTDLSRPFQANVP
                     PGKLFDVTRSVRQLLAVIDKLQPKDTGQFFGWDGERIAW"
     gene            complement(4918..5904)
                     /locus_tag="VD737_01875"
     CDS             complement(4918..5904)
                     /locus_tag="VD737_01875"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXW09340.1"
                     /translation="MHLSRIDLNLLVVFDTVYTEGGITAAGRKLNLSQPAVSHALGRL
                     RELFGEPLFERQGRGIAPTPLARSIAGPVREALAAVERTLGEAGRFEPGTSDRSFRLG
                     LREALEPSVLPRLAHAFAAAGPRLSLATARHDRPRLEQDLAAGEFDVALDVLLPIADR
                     VPHERVLVDRLIVVARKGHPALRRATRRDAWDLEAWLALEHLQVSSRRRGPTLEDMAL
                     RPLGRTRRVRLRCQSHAAACAIAAVTDLVATIPETYAQHHVAADAHRLLPLPLEGLAL
                     ETYMYWSENSAADAANRWLRDQVRRALQSPDATRRRAPRARAAAKSRRAGGD"
     gene            6004..>6712
                     /locus_tag="VD737_01880"
     CDS             6004..>6712
                     /locus_tag="VD737_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXW09341.1"
                     /translation="MNFEYSPKVQDLMRRVDGFMQQHVYPIEHAHDEFVQEPANRWKQ
                     PPVVEELKAKAKADGLWNLFLPHDYGHWSPGLTNLEYAPLAELMGRIEWAPEVFNCSA
                     PDTGNMEVLARYGSPEQQERWLKPLLDGKIRSAYLMTEPQVASSDATNVECSIVRDGD
                     EYVLNGRKWWSSNIYHPHCELLLVMGKTRFDGPRHTQQSTIIVPRNTPGVKVLRPLKV
                     FGEVHSPSGHGEVVLEDV"
BASE COUNT         1126 a         2204 c         2283 g         1099 t
ORIGIN      
        1 cgggcccggg cagctcgctg ccgatcgcgc ggccgaccgc gggctcgagc tcgcggcgct
       61 cgcgccggaa atgctctctg cgctgcgcgg tttccttccg ccgagcacgc agcacggcaa
      121 ccccgtgtac gtgcgcggcg acgcctcggc caggcacttt gcggaggctg cgcgtcacgt
      181 gctcgccgac ggcgccaccg acgcgctgct ggccgtgatg gcgccgttcg tgacgaccga
      241 cccggacgag ttcgcggacg cgctgatcga ggtggcgcgc gcgcagaaga aacccgtgtt
      301 cacgtgctgg atgggcgatg ccgcggtcgt cgagagccgg caacggttcg ccgagaaccg
      361 cattgcgagt tatcgcacgc cggaggccgc cgtggacgcg atcgcggcgc ttgggttgtt
      421 cacggccaac caggagcagc tgctgcaggt gcccgaaccg ctcggggtgg attccgcgcc
      481 ggacaaggcc agggcgcagg cgctgctcga cgaggcgctc gccgccggca acgagtggct
      541 cgaccccggc gaatccaagg aactgctcgc ggcgttcggc atccccgtcg tgcgcgcggt
      601 gccggcgtat tcggccgacg aggcggtgca ggtcgcagag gctgcgggct ttccggtcgc
      661 gatgaagatc ctgtcgcccg acatcgccca caagacggac gtcggcggcg tgcggctcgg
      721 ccgcgccgac gccaggagcg tgcgcgacgg ctacgagaac atgatgcggg acgtcgcgct
      781 cgcgcggccg gacgcccgcc tcgaaggcgt actggtcgag ccgatgtacc tggagaagta
      841 cggccgcgag ctcatgatcg gcgtggtgcg tgatcccgtg ttcgggccgg ccatcagctt
      901 cggcctcggc ggcacgatgg tcgaggtgat ccgcgaccgt gcggtcgcgt tgccaccgct
      961 caaccagttc ctggcccacg acctggtgcg gcgcacgcgt gcgagcctcg cgctgcagtc
     1021 gctgcgtggc gcgcccgccg ccgcgcgtga ggcggtcgag gacgtgctgc tgcgagtctc
     1081 cgagatcgtc tgcgagctgc ctgacgtcgg cgcgatcgac ctgaaccccg tcgtggtgac
     1141 ggcgcagggc gccgtcgtcg tcgatgccag gatcggggtc gtgcgcagcc cgccgggcgc
     1201 gctgccttac cgccacatgg cgatccaccc gtatccgagc gactgtcgtt tcacgatcga
     1261 gttgcccgac ggcgagcgcg ccacggtccg gcccatccgg ccggaggacg cgcgcatgga
     1321 gcaggagttc gtgcaccgcc tgtcggagga gtcgcggttc ctgcgcttca tgttcggcct
     1381 gcacgacctg acgccggcga tgctgtcgcg cttcacgcag atcgactacg accgcgagat
     1441 ggcgctgatc gtcgtccgcc acggcacggg tggcggcgac gacgaagacc ggcagatcgg
     1501 cgtggcgcgc tacatcacgc tgccggacga ggagacctgc gagttcgcga tcgtcgtgag
     1561 cgacgaatgg cagggcaagg ggatcgcgag gcagctcttc ggccacctga tcgacgtggc
     1621 gcgcgcgcgg cgcctcaaga tcatgaccgg catcacgctg cgcgagaact cgcgcatgat
     1681 cgacctgtcg cgtgcgctcg ggttccagac caaggcggac gtcgacgagc ccgagctcgt
     1741 gcggatgacg ctcgcgcttt gaccctgcgc cgggtgaggg ctcttcagtc agcgttcgcc
     1801 ggtgtcccgt tcgacttcgc cggcatccgc cgcgtcgtcg tccgcatcgt cgggcagcag
     1861 ctccgtcgcc tcgtcgccat catcgggtag caagtcgtcg gttctctcgc ctcgcgtcgc
     1921 gtcggttcgg cccggcgctg tcgcggagcg atcttcgtct gcctgctcca cgcccccagc
     1981 gtcgaccgcg cccgccggca gctcgtccgc gtcgatgtcg ccgggttcgg ggcccgcctg
     2041 ccatgcggcg atctcgtcgc gcgagatgcg ccagtccccg ttcacgtcga tctcgcggaa
     2101 ataggccgcc agtctcggct cggcccggtt cagctcggac tgcgacaggg tgccgtcttc
     2161 gtcgacgtcg gcggcggtcc agtcctccgt cgacgtgagg acgtcgtcag catcggacat
     2221 gcccaccggc ggcgactgcg ccgccgcagt acctgccagc agcatcgacg acatcaggac
     2281 tgcggacgtg tttccagtgc gcatgggtgc ttccttgcat gctgtctctt gaaaaggacc
     2341 aaacgcctgc cgcggaggta ccagccttac ctgactttcc gaagccgcgc cccaatccac
     2401 ggagcaatca gtgtggtcac gagtacgagc ggccacaggt tgccaccctg aagcgtgtac
     2461 gcgtcaagta accgctgcca ggaccggtgc tctgcgtaac ccacggcggt ctcgaatcca
     2521 agggtgagca cgagccatat tgcgccgatc caccagaatg agcggtggtg atgcttgccc
     2581 agccaaggtg cagcgagagc agctgccgcc acaatgacgg agcacaacac tacgccgctg
     2641 acaaatcgag ccgatgtcgg accgagtgca tgtacaagaa ccatgtcccg gagcgcgcca
     2701 ttcagcatcg ccagcataag aatgccggcc cagacgagaa ccgatttgag caatgctcga
     2761 gttgtcacgg ctacgattct gacgcccaac gacagttaag gctgcgccgt catacgacga
     2821 ttcgtcgaat aagggagcat gcgcctcgat cgtccctggc catgtgcttc cggccgaaag
     2881 cggacggctg tcaccggcct aaagcggtct caaaatagcg aagaagctgg caactatgcg
     2941 ttattgccat cgggccgcat gtatttgtcg aagttctcga tgtagtcatc gagattttcg
     3001 ttgagcatcg ctcgatattg agaaacgaaa tactcaatgc tttgttcttt gccagctcga
     3061 agatcctcag ccgaatcggc cttgcaagct gtcacccagg aattgaagcg tgccacggcg
     3121 tacatgaacg aggcgctcac tttccctcga ctgacatcgg cgacctgttc attcgccagg
     3181 tcgatgtgcg cgtccgcacg ccggtagaac atctcgtctg tttcggaatc catttatgac
     3241 ttatcccaca actggcgctt cgtgcgaact gagagtgact ggctgcttct ggccgactcc
     3301 gcggttagcc ctggtctaat ctgctctgcg cttcgctgtt gcgatgcagc aaaagggctg
     3361 aaatcggcca ctttcggtcg gtcgtcgcga gattctcggc tggccgcttg tgtacacttg
     3421 tacatactcc gcacatggag tatgaatggg acccggccaa ggcgaaggcc aatgtccgga
     3481 agcaccggat ctcgtttgcc gatgcggcct tggcgctcga ggacccgcga gcgctgaccg
     3541 tggccgatcc ggacgctggc ggcgagcatc gcttcgtcag cctgggttcc gatccgaacg
     3601 gacgcgtgtt ggtgacagtt tttacttcgc gaggcagtcg cacccgaatc atttcttcac
     3661 gcaaggctag ccctaacgag cggcggatat acgagagcaa gtgatgcgca aggaatacga
     3721 tttttcgaag gccaagcgtg gccccgcggt ggcggtgccg tcgggcaaga ctcgaataac
     3781 gattcgcctc gacgatgaca tcctcgaatg gttcaagacg caggtcgaag cggcaggcgg
     3841 cggcaactat cagtcactga tcaacgccgc actgcgtgag cacatgcgca gggcggacga
     3901 accgctcgag aggaccctgc gtcgtgtcct gcgcgaggag ctgaagcgcg caagctgaag
     3961 gaccggctcg agtcgcaagc ggtcgtcgga cgagttgcag aacttcaatg acgaacagcg
     4021 gagccgtgga cgagcggcga atgccacgtg gcgccgtcac caggcgatcc gttcaccgtc
     4081 ccagccgaag aactggccgg tgtccttggg ttgcagttta tcgatcacgg cgagcagctg
     4141 gcgcacggat cgcgtcacgt cgaacagctt gcctggaggt acgttcgcct ggaagggacg
     4201 cgagaggtcg gtgtccgtcg tcccggggtg caacgacacg acgatcacgt tgcggcgtga
     4261 gcgggcccac tcgatcgaga gtgtccggct gatctggttt tgtgccgcct tggcggcgcg
     4321 ataggcgtac cagccgccga gccggttgtc gctgatgctt cccaccttgg ccgagagact
     4381 cgcgaacacg gcgcgttccg ggtgagccag caagggctcg aaatgacgcg cgacgaggag
     4441 gggaccggtc gcgttcaccg cgaagctgtg cgcgagcgcg cccgcgtcga cgtcggcgag
     4501 tcgtttctcg ggtttgaggc cctggctcgc ggcatgcagc acgcccgcgc aattgaccag
     4561 caggtgcagg cgcggcacga cctcggccac cagtttcgcc gctcgcgcga tggaagcgtc
     4621 gtccagcagg tcgagggccg tgaccgtcag ccggccggcg tgatgcgccc gcagttcgtc
     4681 caggccggat gctgatgccg ggttgcggca cgtcgcgacg acgtgggaga cgctgggtac
     4741 cgccagacac tgccggacga actcgagtcc gatgccgcgg ctcgcgccct gcacgagcac
     4801 attgccggcg tggggaaatg aatcgaacat cgaggggctc gtgtcgggga cgctcgatga
     4861 actgttcgta ggcgctggcg gcttggatcc aggcccgcgg gatcagggta agtcttgtca
     4921 gtcccccccg gctcgacgtg atttcgcagc cgcccgcgcc cgcggcgccc tgcgtcgcgt
     4981 cgcatcgggc gactgcaacg cccgtcgcac ctggtcccgc agccagcgat tcgcggcgtc
     5041 ggcggccgag ttctcggacc agtacatgta ggtctcgagc gccaggcctt cgagtggcaa
     5101 cggcagcagg cggtgcgcgt ccgcggcgac gtgatgctgc gcgtacgtct ccgggatcgt
     5161 cgcgacgagg tcggtgacgg ctgcgattgc gcaggcggcg gcgtggctct ggcaccgcag
     5221 tcgcacgcga cgcgtacgac cgagcggacg caaagccatg tcctcgagcg tcgggccgcg
     5281 ccgtcgcgag gacacctgca ggtgctccag ggccagccac gcctccaggt cccacgcgtc
     5341 ccgccgggtg gcacgccgga gcgcggggtg gcccttgcgc gcgacgacga tcaggcggtc
     5401 gaccagcacc cgttcgtgcg gcacgcgatc ggcgatcggc aacagcacgt cgagcgcgac
     5461 gtcgaactcg ccggcggcga ggtcctgctc gagcctcgga cggtcgtgcc gcgccgtggc
     5521 gagcgagagc cgagggccgg ccgctgcgaa cgcatgcgcg agccgtggca gcacgctcgg
     5581 ctcgagcgcc tcgcgcagcc cgaggcggaa gctgcgatcc gaggtgcccg gctcgaaccg
     5641 accggcctcc ccgagcgtgc gctccaccgc ggccaacgcc tcgcgcaccg ggcccgcgat
     5701 cgaccgcgca agcggcgtcg gcgcaatgcc gcgtccctgt cgctcgaaca gcggctcgcc
     5761 gaagagctca cgcagccgtc ccaacgcgtg gctcacggcc ggctgtgaca ggttgagctt
     5821 gcggcccgcg gccgtgatgc cgccttccgt gtagaccgtg tcgaacacga ccagcaggtt
     5881 caggtcgatg cgcgatagat gcatgttgtg aatgattaaa catccaaaca ttgcactagg
     5941 caaatcgaag gcggcgccta cacttccgcc cacgacacct ggcgagccga gggagagtgc
     6001 gccgtgaact tcgaatacag cccgaaagtg caggacctca tgcgccgcgt ggacggcttc
     6061 atgcagcagc acgtctaccc gatcgagcac gcgcacgacg agttcgtgca ggagccggcc
     6121 aatcgctgga agcagccgcc ggtcgtcgag gagctcaagg ccaaggccaa ggcggacggc
     6181 ctgtggaacc tgttcctgcc gcacgactac ggccactgga gcccggggct caccaacctc
     6241 gagtacgcgc cgctcgctga gctgatgggc cgcatcgagt gggcgcccga ggttttcaat
     6301 tgcagcgcgc cggacaccgg caacatggaa gtgctggcgc gctacggttc cccggagcag
     6361 caggagcgct ggctcaagcc gctgctggac gggaagatcc gctccgccta cctgatgacc
     6421 gagccgcagg tggcgtcgtc ggacgcgacc aacgtcgagt gctcgatcgt ccgcgacggg
     6481 gacgagtacg tcctgaacgg ccgcaagtgg tggtcgagca acatctatca cccgcactgc
     6541 gagctgctgc tggtgatggg caagacgcgt ttcgacgggc cgcggcacac gcagcagtcg
     6601 acgatcatcg tgccgcgcaa cacgccgggc gtgaaggtgc tgcggcccct caaggtcttc
     6661 ggcgaggtgc actccccgag cggccacggc gaggtggtgc tcgaggacgt gc
//
LOCUS       DASPFR010000078         5915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1245029, whole genome shotgun sequence.
ACCESSION   DASPFR010000078 DASPFR010000000
VERSION     DASPFR010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245029"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VD737_01885"
     CDS             complement(<1..384)
                     /locus_tag="VD737_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HXW09342.1"
                     /translation="MSLRLIPAIDLRGGRCVRLYQGRFDAETVYDDDPERVLARYREL
                     GARYVHVVDLDGARDGSQANHAVIARLLRACPDVRLQVGGGIRAQAVAEGWLALGVAR
                     VVVGSVAVLQPALVQGWLRALGADRV"
     gene            complement(381..980)
                     /gene="hisH"
                     /locus_tag="VD737_01890"
     CDS             complement(381..980)
                     /gene="hisH"
                     /locus_tag="VD737_01890"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HXW09343.1"
                     /translation="MTADVAIVANGGANIASLRFALDRLGASSELTSDPVVLRRARRV
                     ILPGVGAAGDAMARLRALQLDEVIPTLTQPVLGICLGMQLLFESSDEDDTTCLGLIPA
                     RVTRLVAREGLPVPHMGWNRVEARSHSPLLRGLEDAPHLYFVHSYAAPLGPWTVATTG
                     YGTEFASVVQWRNFSGAQCHPERSSQAGRRLLANFLELQ"
     gene            complement(997..2076)
                     /gene="hisB"
                     /locus_tag="VD737_01895"
     CDS             complement(997..2076)
                     /gene="hisB"
                     /locus_tag="VD737_01895"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HXW09344.1"
                     /translation="MSARARVLFIDRDGTLVEEPPDEQVDRLDKVRLLPGVIPALLEL
                     KRAGYRFVLVSNQDGLGTESFPRPAFEETQDFIRALFASQGIEFDAEFFCPHRSQDGC
                     VCRKPKTGLLTDYLASNPIDAAHSYVIGDRDTDLQLAANLGVQGLRVRADGTDGATWQ
                     AITERLTRPARTATVVRKTRETDIRVRVNLDRTAPVTIATGIGFYDHMLEQVARHGGF
                     ALELTCVGDLQIDEHHTVEDCALALGQALREALGDKVGIARFGFVLPMDEARAQVAVD
                     LSGRAFAVFEGKFGRESVGGLPTELVPHFFRSLADALGAAVHVQVQGDNSHHMVEACF
                     KATGRALRQAVRVEGTELPSSKGVL"
     gene            complement(2081..3136)
                     /gene="hisC"
                     /locus_tag="VD737_01900"
     CDS             complement(2081..3136)
                     /gene="hisC"
                     /locus_tag="VD737_01900"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HXW09345.1"
                     /translation="MSGGSVLGLARPDILQLQPYQHAAWDPSLERMHANEMPWRAQGD
                     NSRAGLNRYPEPQPKALIDRMATLYGASSRNVLIGRGSDEAIDLLARAFCRAGEDKVL
                     ITPPTFGFYKVAARIQGADVIEVPLLRDGFRLDLPATLAAGRGAKLVFLCSPNNPTGN
                     LLDEDAMLRLCRELAQSALVVVDEAYLEFSGRASLAGRLAEFPNLVVLRTLSKAYALA
                     GARLGTLIASEEIVGLLKRVIPPYAIPASTVDEVLALTEAPQRASAAARIRTLLDERT
                     MVKERLERSRNVMRVYPSDANFLLVECRDAPGFLAAGKASGLLVRDFSASPGLANCLR
                     VSIGTPEQNRRLLAAVE"
     gene            complement(3133..4458)
                     /gene="hisD"
                     /locus_tag="VD737_01905"
     CDS             complement(3133..4458)
                     /gene="hisD"
                     /locus_tag="VD737_01905"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HXW09346.1"
                     /translation="MNRPLEILDWAALDEAGRDAILRRPAQRDAASLLERARRIVEDV
                     RARGDEALRAYSESLDRVRLESFDVTVEEFEHAEGALTQEQHAALRRAIDTVSRFHEL
                     QVPGPLRLETAPGVVCERMTVPLDAVGLYVPAGTAPLPSTAVMLAVPARIAGCPVRVM
                     CTPPRPDGRADPAVLVAARLCGVERVFKLGGAQAVAAMAYGTATVPKVVKIFGPGNAW
                     VTAAKQLVAQDADGAALDMPAGPSEVLVVADETARADFVAADLLAQAEHSPDAQVMLV
                     TTSRAFAGSCVREVERQLAALPRRDVAARAIAESRVIVVADLETAMAVSNRYAPEHLI
                     LQVARPRALLASVRNAGSVFLGAWTPETMGDYCSGTNHVLPTYGHARAYSGLGVPDFV
                     KRITVQELSPQGLATLGPTARTLARLESLDAHAHAVTVRLDALAAQAAS"
     gene            complement(4455..5354)
                     /gene="hisG"
                     /locus_tag="VD737_01910"
     CDS             complement(4455..5354)
                     /gene="hisG"
                     /locus_tag="VD737_01910"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HXW09347.1"
                     /translation="MDRQERLRIAVQKSGRLTDESLDLLHRCGLKFSRGKDQLMCYGE
                     NMPLDVLFVRDDDIPDLVQEDVCDLGLVGLNVLEEKRLAMAARGTPPRIEVLQKLDFG
                     RCRLVLAVPEDFRYENLRSIAGKRIATTYPNVLARFLRDNDIDAHVVTLSGAVEIAPR
                     LGRADVICDLVSTGSTLTANHLREVAVVLESQAALVRTPVALPDVKADWVRRLLVRID
                     GVQQVRESKYIMLHAPRAALAQIKLLLPGSESPTVIPLDGANDKVAVHAVCRETVFWE
                     TLERLKAAGASAVLVLPVEKMLA"
     gene            complement(5363..5668)
                     /locus_tag="VD737_01915"
     CDS             complement(5363..5668)
                     /locus_tag="VD737_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HXW09348.1"
                     /translation="MKKHPGISPRQEAQAERALYQAILTLRSVEECRDFFKDLCTPAE
                     LQALADRWTVVGLLQQGRPYREISKQTGVSVTTIGRVARYLEAGHGGYALAASRLGA"
BASE COUNT          862 a         2055 c         2135 g          863 t
ORIGIN      
        1 cacgcgatcg gcgccgagcg cccgcagcca tccctgcacg agcgccggct gcagcaccgc
       61 gacgctgccg accacgacgc gcgccacgcc gagcgcgagc cagccctcgg ccaccgcttg
      121 cgcgcggatg ccgccaccca cctgcagccg cacgtccggg caggcgcgca ggagtcgcgc
      181 gatcaccgcg tggttggcct gcgagccgtc gcgcgcgccg tcgaggtcta cgacgtggac
      241 gtagcgagcg ccgagttcgc ggtaccgggc gagcactcgc tccgggtcgt cgtcgtagac
      301 cgtctcggcg tcgaagcgac cctggtacag ccgcacgcag cggccgccgc gcaggtcgat
      361 ggcggggatg aggcgcaggc tcactgcagc tccaggaaat tggccagcag ccgccgcccc
      421 gcctgcgacg accgctcggg gtggcactgt gcgccgctga agttgcgcca ctgcacgacg
      481 gacgcgaact ccgtgccgta cccggtagtg gccaccgtcc acggtccgag gggggccgca
      541 tagctgtgca cgaagtacag gtgcggcgcg tcctcgagac cgcgcagcag cggcgaatgc
      601 gatcgcgcct cgacgcggtt ccagcccatg tgcggcaccg gcaggccttc acgcgccacg
      661 agccgcgtga cgcgcgccgg gatcaggccg aggcaggtcg tgtcgtcttc gtcgctcgac
      721 tcgaacagca gctgcatgcc gaggcagatg ccgagcaccg gctgcgtgag cgtcgggatt
      781 acctcgtcca gttgcaacgc gcgcaggcgt gccatcgcat ccccggcggc gccgacgccc
      841 ggcaggatta cgcgacgcgc gcgacgcagg acaaccggat cgctcgtcag ctccgacgag
      901 gcgccgagcc ggtcgagcgc gaagcgcagc gacgcgatgt tggcgccgcc gttcgcgacg
      961 atcgccacgt cggcggtcat cggtcccgtc cgtcggtcac aggacgccct tgctgctcgg
     1021 cagctccgtg ccctcgacgc gcacggcctg gcgcagcgcg cggccggtcg ccttgaagca
     1081 ggcctcgacc atgtggtgcg agttgtcgcc ctgcacctgg acgtgcacgg cggcgccgag
     1141 cgcgtcggcc agcgagcgga agaagtgcgg cacgagctcg gtcggcagcc cgcctaccga
     1201 ctcgcggccg aacttgccct cgaacacggc gaaggcacgc ccggagaggt cgacggcgac
     1261 ctgcgcgcga gcctcgtcca tgggcagcac gaagccgaac cgcgcgatgc cgaccttgtc
     1321 gccgagcgcc tcgcgcagcg cctggccgag cgcgagcgcg cagtcctcga ccgtgtggtg
     1381 ctcgtcgatc tgcaggtcgc cgacgcaggt gagctcgagc gcgaagccgc cgtgccgggc
     1441 gacctgctcg agcatgtggt cgtagaagcc gatgccggtc gcgatcgtca cgggtgcggt
     1501 gcggtcgagg ttcacgcgca cgcggatgtc ggtttcgcgg gtcttgcgca cgacggtggc
     1561 ggtcctggcc ggccgggtca ggcgttcggt gatggcctgc caagtggcgc cgtcggtgcc
     1621 gtccgcgcgt acgcgcaggc cctgcacgcc aagattcgcg gcgagctgca ggtcggtgtc
     1681 gcggtcgccg atcacgtagc tgtgcgcggc gtcgatcgga ttggaggcga ggtagtcggt
     1741 gagcagcccc gtcttcggct tgcgacagac gcagccgtcc tgggatcggt gcgggcagaa
     1801 gaactccgcg tcgaactcga tgccctggga ggcgaacaag gcgcgaatga agtcctgtgt
     1861 ctcctcgaac gccggacggg ggaacgattc cgtaccgagc ccgtcctggt tgctcacgag
     1921 cacgaagcgg tagccggcac gcttgagctc cagcagcgcc ggaatcacgc ccggcagcag
     1981 gcgcaccttg tccaggcggt cgacctgctc gtcgggcggc tcctcgacga gcgtgccgtc
     2041 gcggtcgatg aacagcacgc gggctctggc gctcatcggg tcactccacg gcggcgagca
     2101 ggcgccggtt ctgttccggc gtgccgatcg acacgcgcag gcagttcgcc aggcccgggc
     2161 tcgccgagaa atcacgcacc agcaggccgc tcgccttgcc cgccgcgagg aagcccggcg
     2221 cgtcccggca ctcgaccagc aggaagttcg cgtcggacgg gtacacgcgc atcacgttgc
     2281 gggaccgttc gagccgctcc ttcaccatcg tccgctcgtc gagcaacgtg cggatccgcg
     2341 cggcagccga cgcgcgctgt ggcgcctctg tgagcgccag cacctcgtcg acggtggaag
     2401 cggggatcgc atacggcggg atgacgcgct tgagcaggcc gacgatctct tcgctcgcga
     2461 tcagcgtgcc cagacgggcg ccggccagtg cataggcctt ggacagcgtc ctcaggacca
     2521 ccaggttcgg aaactcggcg agccgcccgg cgaggctcgc gcggccggag aactcgaggt
     2581 acgcctcgtc gacgacgacc agggcacttt gcgcgagttc gcggcatagc cgcagcatcg
     2641 cgtcctcgtc gagcaggttg ccggtcggat tgttcggtga gcacaggaac accagcttgg
     2701 cgccgcgtcc ggccgccagc gtcgccggca ggtcgagccg gaaaccgtcg cgcagcaacg
     2761 gcacctcgat cacgtccgca ccctggatgc gcgcggccac cttgtagaac ccgaacgtcg
     2821 gtggcgtgat cagtaccttg tcctcaccgg cccggcagaa cgcacgcgcg agcaggtcga
     2881 tcgcctcgtc actgccgcgc ccgatgagca cgttgcgact cgacgccccg tacagcgtcg
     2941 ccatgcggtc gatgagcgcc ttcggctgcg gctccggata gcggttcagg cccgcgcgcg
     3001 aattgtcgcc ctgggcgcgc cagggcattt cgttcgcgtg catgcgttcg agcgagggat
     3061 cccaggcggc gtgctggtag ggttgcagct gcaggatgtc cggccgcgcg aggccgagta
     3121 ctgaaccgcc gctcatgagg cagcctgcgc cgcgagcgcg tcgagacgca cggtcacggc
     3181 gtgcgcgtgg gcgtccagcg actcgagccg tgcgagcgtg cgtgcggtcg ggccgagcgt
     3241 cgccaggccc tgcgggctca gttcctggac ggtgatgcgc ttgacgaagt ccgggacgcc
     3301 gagcccgctg taggcccgcg cgtggccata ggtcggcagc acgtggttgg tgccgctgca
     3361 gtagtcgccc atcgtttcgg gcgtccaggc gccgaggaac accgagcccg cgttgcgcac
     3421 gctcgcgagc agcgcgcgcg gcctggcgac ctgcaggatc aggtgctcgg gcgcatagcg
     3481 gttgctgacg gccatggcgg tctcgaggtc ggccacgacg atcacgcggc tttcggcgat
     3541 cgcacgggcg gcgacgtcgc gccggggcag ggcggcgagc tgccgctcga cttcccggac
     3601 gcacgacccg gcgaacgcac gcgaagtggt caccagcatg acctgcgcgt cggggctgtg
     3661 ctcggcctgc gcgagcaggt ctgcggcgac gaagtccgct cgcgcggtct cgtcggccac
     3721 cacgagcacc tcggacgggc ctgcgggcat gtcgagcgcg gcgccgtcgg cgtcctgcgc
     3781 gaccagctgc ttcgcggccg tgacccaggc gttgcccgga ccgaagatct tgacgacctt
     3841 ggggacggtg gccgtgccgt aggccatggc cgccaccgcc tgcgcaccac ccagcttgaa
     3901 cacgcgttcg acgccgcaga gtcgtgcggc gacgaggacg gccggatcgg cgcgtccgtc
     3961 cggtcgcggg ggcgtgcaca tcacgcgcac cgggcacccg gcgatgcggg ccggcacggc
     4021 gagcatgacg gcggtggagg gcaggggggc cgttccggcc ggcacgtaca ggccgaccgc
     4081 gtcgagcggc accgtcatgc gttcgcagac gacgcccggc gcggtctcga ggcgcagcgg
     4141 accgggcacc tgcagttcgt gaaaccgcga gacggtgtcg atcgcgcggc gcagcgccgc
     4201 gtgctgctcc tgggtcaggg cgccctcggc gtgttcgaac tcctcgacgg tgacgtcgaa
     4261 cgattcgagg cgcacgcggt ccagcgactc ggaataggcg cgcaacgcct cgtcgccgcg
     4321 ggcgcgcacg tcctcgacga tgcgacgcgc tcgctcgagc aggctcgccg cgtcgcgctg
     4381 cgcaggccgg cgcaggatgg cgtcgcgacc cgcttcgtcg agtgccgccc agtccaggat
     4441 ctcgagcggt ctgttcatgc cagcatcttc tcgaccggca acaccagcac cgcgctcgcg
     4501 ccggccgctt tcaggcgctc gagcgtctcc cagaacacgg tctcacggca gacggcgtgc
     4561 acggccacct tgtcgttggc accgtcgagc ggaatcacgg tgggtgattc gctgccgggc
     4621 agcagcagct tgatctgggc cagcgccgcg cgcggggcgt gcagcatgat gtacttgctc
     4681 tcccgcacct gctgcacgcc gtcgatgcgg accagcaggc gccgaaccca gtcggccttg
     4741 acgtccggca gggccacggg cgtgcgcacg agcgccgcct ggctctccag gacgaccgcg
     4801 acctcacgca ggtggtttgc ggtcagggtc gagccggtgg agacgaggtc gcagatcacg
     4861 tcggcgcgac cgaggcgcgg cgcgatctcg accgcgccgg acagcgtgac gacgtgagcg
     4921 tcgatgtcgt tgtcgcgaag gaagcgggcg agcacgttgg ggtacgtcgt cgcgatgcgc
     4981 ttgcccgcga tgctgcgcag gttttcgtac ctgaaatcct caggcacggc gagcaccagc
     5041 cggcagcgtc cgaagtcgag cttctgcagc acctcgatcc ggggcggcgt gccgcgcgcg
     5101 gccatcgcca gccgcttctc ctcgagcacg ttcaggccga cgaggccgag gtcgcagacg
     5161 tcttcctgca cgaggtcggg gatgtcgtcg tcacggacga acagcacgtc gagcggcatg
     5221 ttctccccgt aacacatcag ctggtccttg ccgcggctga acttgaggcc gcagcggtgc
     5281 agcaggtcca gcgactcgtc ggtgagccgg cccgacttct gcacggcgat ccgcaaacgt
     5341 tcctgcctgt ccatcgaggt tcctatgctc cgaggcgact cgccgccagc gcgtatccgc
     5401 catggccggc ttcgaggtaa cgggcgacgc gcccgatcgt ggtgacgctc acgccggtct
     5461 gcttcgagat ctcgcggtag ggccgcccct gctgcagcag tccgacgacg gtccagcgat
     5521 cggcgagcgc ctgcagttcg gcgggcgtgc acaggtcctt gaagaagtcg cggcactcct
     5581 cgacggaacg cagcgtcagg atcgcctggt acagggcgcg ttcggcctgc gcctcctggc
     5641 gcggcgaaat gccggggtgc ttcttcacgg gtggtcgggt gcggggctgg gggcggcgaa
     5701 tgtattagcg tattagcacg ttaatacatt ggatcgtcga ctgcaagcgt gttctccgcg
     5761 gagcgggcgg tgtgcggcgc gatccgcccc tgtccggaca agttccgggg acgccctgaa
     5821 cccatccctg ggggctccgg ccgcgacatc cctgtcgcgg acggtcccgg aacccaggtc
     5881 gaaccggatc gggccgcgcc ttcggtgttg gtgct
//
LOCUS       DASPFR010000079         2203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1366951, whole genome shotgun sequence.
ACCESSION   DASPFR010000079 DASPFR010000000
VERSION     DASPFR010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366951"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1690
                     /locus_tag="VD737_01920"
     CDS             <1..1690
                     /locus_tag="VD737_01920"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HXW09349.1"
                     /translation="PGSYPYAGGIYPYRREDEDPTRMFAGEGMPERTNRRFHYLAAGH
                     KSARLSTAFDSTTLYGEDPDERPDIYGRIGNSGVSIATLDDMKRLYSGFDLCATSTSV
                     SMTINGPAPMILAMFMNTAVDQQVERWLREQGRWDEAERKVAALFEGRERPRYRGELP
                     AGHDGSGLGLLGVTGDQLVDAETYARIRKETLSLVRGTVQADILKEDQAQNTCIFSTE
                     FALRMMGDVQQFFIENDVRNFYSVSISGYHIAEAGANPISQLAFTLANGFTIVEYYLA
                     RGMKIDDFAPNLSFFFSNGMDPEYAVIGRVARRIWARAMRDLYRAGPRSQMLKYHIQT
                     SGRSLHAQEIQFNDARTTLQALYALFDNCNSLHTNAYDEAITTPTEESVRRAVAIQLI
                     INKELGLNKTQNPWQGSFAVEQLTDLVEEAVYREFDRLSERGGVLGAMETMYQRGRIQ
                     EESLYYEQLKHDGTLPIIGVNTYLANDDATEANREAELIRSTEEEKQDQVQAVRAFLD
                     RNADRRPAALEQLQRTAAGKGNAFGALMDAVKVASLGSISHALYQVGGQYRRSM"
BASE COUNT          382 a          739 c          758 g          324 t
ORIGIN      
        1 gccgggctcg tatccctacg ccggcggcat ctacccgtac cggcgcgagg acgaggaccc
       61 gacccggatg ttcgcggggg agggcatgcc ggaacggacc aaccggcgct tccattacct
      121 cgcggctggc cacaagtcgg cgcggctgtc gacggccttc gactcgacga cgctgtacgg
      181 cgaggatccc gacgagcgcc cggacatcta cggacgcatc ggcaactccg gcgtgtcgat
      241 cgcgacgctg gacgacatga agcggcttta ctcgggcttc gacctgtgcg ccacttccac
      301 gtcggtgtcg atgaccatca acggcccggc gccgatgatc ctcgcgatgt tcatgaacac
      361 cgcggtcgac cagcaggtcg agcgctggct gcgcgagcag ggccgctggg acgaggcgga
      421 gcgcaaggtc gccgcgctgt tcgagggccg cgagcggccc cgttaccggg gcgagctgcc
      481 ggccgggcac gacggctcgg ggctcggcct gctcggcgtc accggcgacc agctcgtcga
      541 tgccgagacc tacgcgagga tccgcaagga gacgctgtcg ctggtgcgtg gcacggtgca
      601 ggccgacatc ctcaaggagg accaggcgca gaacacctgc atcttctcga cggaattcgc
      661 gctcaggatg atgggcgacg tgcagcagtt cttcatcgag aacgacgtcc gcaacttcta
      721 ttcggtctcg atctcgggct atcacatcgc ggaggccggc gcgaacccga tctcgcaact
      781 cgcgttcacg ctcgcgaacg gtttcacgat cgtcgagtac taccttgcgc gcggcatgaa
      841 gatcgacgac ttcgcgccga acctgtcgtt cttcttctcg aacggcatgg acccggagta
      901 cgcggtgatc ggccgcgtcg cacggcgcat ctgggcgcgc gcgatgcgcg atctctaccg
      961 agccggcccg cgctcgcaga tgctcaagta ccacatccag acgtccggcc ggtcgctgca
     1021 cgcgcaggag atccagttca acgacgcgcg cacgacgctg caggcgctct acgcgctgtt
     1081 cgacaactgc aacagcctgc acaccaacgc gtacgacgag gcgatcacga cgccgaccga
     1141 ggaatcggtg cgtcgcgcgg tggcgatcca gctgatcatc aacaaggagc tcgggctcaa
     1201 caagacgcag aacccatggc agggctcgtt cgccgtcgag cagctcaccg acctggtcga
     1261 ggaagcggtc tatcgcgagt tcgaccgcct ctcggagcgc ggcggcgtgc tcggcgcgat
     1321 ggagacgatg taccagcggg gccgcatcca ggaggagtcg ctctactacg agcagttgaa
     1381 gcacgacggc acgctgccga tcatcggcgt caacacgtac ctcgcgaacg acgacgcgac
     1441 ggaggcgaac cgcgaggcgg agttgatccg ctcgacggaa gaggagaagc aggaccaggt
     1501 gcaggcagtg cgcgcgttcc tcgatcgcaa tgcggatcgc cgcccggcgg cgctcgagca
     1561 gttgcagcgc acggccgcgg gcaagggcaa cgccttcggc gcgctgatgg acgcggtcaa
     1621 ggtcgcctcg ctgggcagca tctcgcacgc gctctaccag gtgggcgggc agtaccggcg
     1681 cagcatgtag ttcgcctcgc ggcagcgctg gcgcgaggag tgagccgctg ctgcagggct
     1741 gcgtgcgacg ctggtgcgag gagtagccgc tgcagggctg cgtgagacgc cggcgcgagg
     1801 agtgggccgc tgcaggcggc gacccgtgcg ccgcattccg gtctgcggga cgccgtgaac
     1861 ccatccctgg gggctccggc cgcggcatcc atgccgcgga cggcccgccg accggaatcc
     1921 gacgcacgga tcaccgccac cagccgccgc gtgcgcatcg aggcgcgcat ccatgaacgc
     1981 tgcgtgtata tcacgcacgc acgcatgcag ctctgttctg cgcacgcatc aacggctgca
     2041 agcgtttaat cccacacgac gtcgcagcgc gcgcgtcgac ggctgacggg ttgcttgccg
     2101 ggccgtcgcg caacaggacg ttgcgcgcgg agcctccagg gatggattta cggcgtcccg
     2161 gcaagcaatt cgtcaggcgg cggcgtgggc gcgcatgctc cct
//
LOCUS       DASPFR010000080         3218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1367699, whole genome shotgun sequence.
ACCESSION   DASPFR010000080 DASPFR010000000
VERSION     DASPFR010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367699"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VD737_01925"
     CDS             <1..638
                     /locus_tag="VD737_01925"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXW09350.1"
                     /translation="DCATGNGQAAVALAAHFDEVLAVDASDAQLAQAQPHPRVRYERA
                     LAEALPVADGQVALLAVAQAIHWFDFDRFHAECRRVLMPGGVLAAWTYTVFRAGGAID
                     EVVDRFYAGTVGPYWPPERAWVQQAYRTIPFPWDEVAPPPFRLQTEWTLEQVLGYFAS
                     WSAVQQYRAQRGGEDPLPAVERELGSVWPAGSTVRLDWPLYMRVGRQGPRL"
     gene            688..2757
                     /locus_tag="VD737_01930"
     CDS             688..2757
                     /locus_tag="VD737_01930"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HXW09351.1"
                     /translation="MRWTLPLALTLTLVACANAPKSTEPAAATPPQATEPPAPTLASG
                     INLENFDRNVRPQDDFYRYVNGTWLAQTEIPADKNNYGAFSILQDEAEKNLHVIVEEA
                     ARSNAAAGTDAQLIGDFYTSYMDEAKAESLGLEPLRPELARIAAIQDRTALVDYLARA
                     QLVLIDTPVSASVIEDAKNPEVWALWLTQSGLGLPERDYYFSKEKRFAEIRAAYLAHI
                     GNVFTLAGRKDAAAVARKLMDFETRLAEASWPAVRMREFQKLYNPVDVAAATKASSGF
                     DWSRWLAQMGLGHVRTVSLGQPDYFEAVGKAVAEVPLATWRDYLTLRTIDSFSPYLSK
                     AFVEENFDFYARTLSGTPELKPRWKRALGELEGSTGDLLGKEYVKRHFPPQAKQRMEA
                     LVANLLRAFDASIDELEWMSPQTRKEAHDKVANFTVKVGYPVKWKEYPGLVTRPDDLV
                     GNVLRARDVNVQREIAKVGNPVDKTEWGLTPQTVNAYYNPLANEIVFPAAILQPPFFD
                     MNADDAVNYGGIGAVIGHEISHGFDDRGREFDGKGVLRDWWQPADADKFNSLAGLLVK
                     QYDAFSPLPGFNVNGQLTLGENIGDLSGLAVAYKAWQLSLQGQPSPVIDGFSGEQRFF
                     LGWGQIWARKYRDDELIKRLKTDPHSPAQYRCNGILRNSPAFAKAFDVKPGDQMYLPP
                     EQQVRIW"
     gene            2808..>3218
                     /locus_tag="VD737_01935"
     CDS             2808..>3218
                     /locus_tag="VD737_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HXW09352.1"
                     /translation="MRALQGLWLAAVALLLSGCGYNTIQVQDEQTKSAWSEVLNQYQR
                     RADLIPNLVNTVKGFAAQEQAVLIGVTEARAKATQVRVDPGDPASLQQFQAAQGELSS
                     ALARLMVVVERYPELKSDQNFRDLQAQLEGTENRI"
BASE COUNT          531 a         1110 c         1098 g          479 t
ORIGIN      
        1 tcgattgcgc aaccggcaac gggcaggcgg ccgtcgcgct cgcggcccac ttcgacgagg
       61 tgctggccgt cgatgcgagc gacgcccaac tcgcccaggc gcagccgcac ccgcgcgtcc
      121 ggtacgagcg tgcgctcgcg gaagcgctgc cggtcgccga cggccaggtc gcgctgctcg
      181 ccgtggcgca ggcgatccac tggttcgact tcgaccggtt ccacgccgag tgccgccgcg
      241 tgctgatgcc cggcggcgtg ctcgccgcgt ggacgtacac ggtcttccgg gccggaggcg
      301 ccatcgacga ggtcgtcgac cgtttctacg caggcacggt gggcccgtac tggccgcccg
      361 agcgggcgtg ggtgcagcag gcgtaccgga cgatcccgtt tccgtgggac gaggtcgcgc
      421 cgccgccgtt ccggctgcag accgagtgga cgctcgaaca ggtgctcggc tatttcgcca
      481 gctggtccgc cgtgcagcag tatcgcgcgc aacgcggcgg cgaggatccg ctgccggccg
      541 tggagcggga gctcggctcg gtctggcccg ccggaagcac ggttcgtctc gactggccgt
      601 tgtacatgcg agtcggtcgc cagggtcctc gcctataatc ggcggcttcg tggcggcggt
      661 cccgccatcc ctcctggagt gacgttcatg cgctggacgc tgccgcttgc cctcacgctc
      721 acgctcgtcg cgtgcgccaa tgcgcccaag tcgaccgagc ccgccgcggc cacgccgccg
      781 caggccaccg aaccgcccgc gccgacgctg gcctcgggga tcaacctcga gaacttcgac
      841 cgcaacgtgc ggccgcagga cgacttctat cgctacgtga acggcacgtg gctcgcgcag
      901 acggagatcc ccgccgacaa gaacaactac ggcgcgttct ccatcctgca ggacgaggcc
      961 gagaagaacc tgcacgtgat cgtcgaggaa gccgcccgca gcaacgctgc ggccggcacc
     1021 gacgcgcagc tgatcggcga cttctacacg agctacatgg acgaggcgaa ggccgagagc
     1081 ctgggtctcg agccgctccg gcccgagctc gcccgcatcg ccgcgatcca ggaccgcacg
     1141 gcgctggtcg actacctggc gcgcgcgcag ctcgtgctga tcgacactcc ggtcagcgca
     1201 tccgtgatcg aggacgccaa gaacccggag gtgtgggcgc tgtggctgac ccagtcaggc
     1261 ctggggctgc ccgagcgcga ctactacttc tcgaaggaga agcgcttcgc cgagatccgt
     1321 gcggcgtacc tcgcgcacat cggcaacgtc ttcacgctcg ccggccgcaa ggatgccgcg
     1381 gcggtcgcca ggaaactgat ggacttcgag acgcggctcg ccgaggccag ctggcccgcg
     1441 gtgcgcatgc gcgagttcca gaagctctac aacccggtcg atgtcgcggc ggcgaccaag
     1501 gcttcgtcgg gcttcgactg gtcgcgctgg ctcgcgcaga tgggcctcgg gcacgtcagg
     1561 accgtatcgc tcggccagcc cgactacttc gaggcggtcg gcaaggccgt cgccgaggtg
     1621 ccgctggcga cgtggcgcga ttacctcacg ctgcgcacga tcgacagctt ttcgccctac
     1681 ctgagcaagg cgttcgtcga ggagaacttc gacttctacg cgcgcacgct gtccggcacg
     1741 ccggagctca agccgcgctg gaagcgggca ctcggcgagc tcgaaggctc cacgggcgac
     1801 ctgctgggca aggagtacgt caagcgtcac ttcccgccgc aagccaagca gcgcatggag
     1861 gcgctcgtcg ccaacctgct gcgtgcgttc gatgcctcga tcgacgagct cgaatggatg
     1921 agcccgcaga cgcgcaagga ggcgcacgac aaggtcgcga acttcacggt caaggtgggt
     1981 tatcccgtca agtggaagga gtaccccggc ctcgtgacgc ggccggacga cctggtcggc
     2041 aacgtgctgc gcgcgcgcga cgtcaacgtg cagcgcgaaa tcgccaaggt cggcaatccg
     2101 gtcgacaaga ccgagtgggg cctgacgccg cagacggtca acgcctacta caacccgctc
     2161 gccaacgaga tcgtcttccc ggcggcgatc ctgcagccgc cgttcttcga catgaacgcg
     2221 gacgacgcgg tcaactacgg cggcatcggc gccgtgatcg gccacgagat cagccacggc
     2281 ttcgacgacc gggggcgcga attcgacggc aagggcgtgc tgcgcgactg gtggcagcct
     2341 gccgacgccg acaagttcaa ctcgctcgcg ggcctgctcg tcaagcagta cgacgcgttc
     2401 agcccgctgc ccggcttcaa cgtcaacgga cagctcacgc tcggcgagaa catcggtgac
     2461 ctctccggcc tcgccgtggc ctacaaggcc tggcagctgt cgctgcaggg ccagccctcg
     2521 ccggtcatcg acggtttctc gggcgagcag cgcttcttcc tcggctgggg ccagatctgg
     2581 gcgcgcaagt accgcgacga cgagttgatc aagcggctca agaccgaccc gcacagcccg
     2641 gcgcagtacc gctgcaatgg catcctgcgc aacagtcctg cattcgcgaa ggctttcgac
     2701 gtgaagccgg gcgaccagat gtacctgccg cccgagcagc aggtgcgaat ctggtaaggt
     2761 cgccgtccgt gtcaggggtg cgtttcggtt cataaggaga tcgtccgatg cgtgcgttgc
     2821 agggcctgtg gctggcggcc gtcgcgctgc tgttgtccgg ttgcggctac aacacgatcc
     2881 aggtgcagga cgagcagacc aagtcggcct ggtccgaggt gctgaaccag taccagcggc
     2941 gggccgacct gatcccgaac ctcgtcaaca ccgtcaaggg gttcgcggcg caggaacagg
     3001 ccgtgctgat cggcgtgacc gaggcgcggg ccaaggcgac gcaggtcagg gtggatccgg
     3061 gcgacccggc gtcgctgcag cagttccagg ccgcgcaggg cgagctctcg agcgcgctcg
     3121 cgcggctcat ggtcgtcgtc gagcgctacc cggagctcaa gtcggaccag aacttccgcg
     3181 acctgcaggc ccagctcgag ggcaccgaga accggatc
//
LOCUS       DASPFR010000081         9866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_148197, whole genome shotgun sequence.
ACCESSION   DASPFR010000081 DASPFR010000000
VERSION     DASPFR010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9866
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148197"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VD737_01940"
     CDS             <1..551
                     /locus_tag="VD737_01940"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXW09353.1"
                     /translation="DGWESRGEAIAVYGRRNRGFEMSRAMTAWSDDIVLCTHGAAGLN
                     AEQKRQLARNGVRIVEEKIERLTGRDGQLEAIVFRGGRRLARRRLFFDLPSHAQSDLS
                     RQLGCRLRRNGSVHCSGYEATDVPGVFIAGNIAGDVQLVIFAAAEGAKAAFGINRSLT
                     REQFELNATGRRIVEHPTRESG"
     gene            complement(555..1277)
                     /gene="bcsS"
                     /locus_tag="VD737_01945"
     CDS             complement(555..1277)
                     /gene="bcsS"
                     /locus_tag="VD737_01945"
                     /inference="COORDINATES: protein motif:HMM:NF028346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsS"
                     /protein_id="HXW09354.1"
                     /translation="MNIRDAWSTTFRALVVAVLGSGSMLAGPRAVAADRLLLVGAEAA
                     DAAYYSYVGAILPFGERRDGRGWYQRYWVDAFGYEYDGGPGRVEADAYGVEAALGYGG
                     SSDLGWWSVSAGLRHTDTQLEPDDRRANARGSQLGGKLQFDFERTVAQDWRIAGIASY
                     SNEQNGYWLRGRLMHGTTQARSFGFEAVANGNDEADATAAGFVTTFRPGNARYTFGLK
                     AGYRFQDDADGAYGGLEFGYAF"
     gene            complement(1274..3475)
                     /locus_tag="VD737_01950"
     CDS             complement(1274..3475)
                     /locus_tag="VD737_01950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXW09355.1"
                     /translation="MSDGSNSGAVAFPPSRLASLTLAQRALVFTFLGVGLWYLAWRPS
                     AFNPEAPLFSALVYGAEVFGYLCAVLYLGMCWRLRQRTSLPVPEGATVAVFVPTINES
                     VEIVRRTVMAAQRMRNATEVWLLDDGNRLEMQVLADELGCRYLARTENTHAKAGNLNH
                     ALGHTRTEFVAIFDADHAPAPNFLDETLGFFSDPKVAFVQTPQDFYNLDSFQHRLNRR
                     DSLVWSEQTLFFRVIQPGKDSQDAAFFCGSCAVVRRTALDDIGGFSTGTVTEDIHTSI
                     RLHKRGWKSVYYGRSLAFGLAPASIIPFLKQRLRWGQGAMQVWRQEGVLFTRGLTLRQ
                     RLSYLATMLAYFEGWQRLVFFTAPVIVLTTGTMPILQLDREFLVRFLPYYLLTFWTFE
                     EVARGHGRTVLTEQYNMVRFAVFITATFGFFLRKLRFVVTPKQMGEGDATRRMLWPQY
                     LVLAANVVAIPVGIAWFGTERGLPAGALIANLIWAALTCGIAVAAIRFALQSGSSRRR
                     EYRFPLPVPLRLQDESGRESVALATDISPQGCRVVGAAAGAASVGQELRGELLLTTGA
                     LPVRAMVRAVNAPGADDPRPGEPALGCEFRWGVSDERNRLEMFLFGSDLQWQLNGLTD
                     RVATPLERVRGWLRPGLAEQSRRLAGETWAPVLYKRVNSEHGDGVGFISRSDPRTGVR
                     TVVSMGMLPQNGRLYAEEVTATGPRGVVGRVQDVAVLETHAAPIYLYKLTA"
     gene            3741..5918
                     /locus_tag="VD737_01955"
     CDS             3741..5918
                     /locus_tag="VD737_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXW09356.1"
                     /translation="MEAADDNDEFPGQPHDRRREPVRIDPALGRAQERFMEMIASGAP
                     VRSVLEAIACEIQALVPGTQVCVLVVDSTGTSLRHGAAPTLPPEFLREVDGLPIGPAS
                     GACGTAAYRRAPVVVEDIATDPLWDDFRALAQQHGLRSAWSSPICDGQGGVLGTLAFY
                     HCERWTPGSYEQSVAAMATHAAAVALRRDREEQQLRKLSAAVEQSADSIVITDLRGVI
                     EYVNEAFCRVSGYSRDEVVGSTPAILKSGRTPAATYRELWETLVRGESWRGEFVNRRK
                     DGREYHESSLITPLRQPDGRITHYLAVKSDVTEHRRISAELALHRHHLEDLVEKRTAE
                     LALAKVQAESANRAKGVFLANMSHEIRTPMNSILGLTYILRQHTSDPEQQALLARLHD
                     SAQHLMHVINGILDLSKIEAGKIAIEESELSVENVLRNVVELLSHEAEAKNLVLDVEV
                     HGVDGVLYGDPTRLSQALLNYVSNAIKFTPSGRILLRARVAREDANSQCVRFEVHDTG
                     PGIAEDQLGKLFNAFEQGDASTTRRHGGTGLGLAITRGLAQMMGGDAGAESTHGAGST
                     FWFTATLGRLAPSTITSESALREFRSSQRPAVKSAAREFSGRVLLAEDNPTNQFVLLR
                     LLRDLGVSVDLAENGRDAVELLRERPYDLVLMDVQMPEMDGLAATRAIRELPQREQTP
                     IVALTANAFTDDRERCLQAGMNGFLPKPIDPTLLREALERWLR"
     gene            5928..6740
                     /locus_tag="VD737_01960"
     CDS             5928..6740
                     /locus_tag="VD737_01960"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXW09357.1"
                     /translation="MTTEFSRVLASIDDGGDGSLRVAVPPDWLQGRTVFGGMQMAIAV
                     RGMRSSMPETARTLPLRSVQMTFVGPLTGSDPVQLRSELLRQGRSITHARCDLRQSGS
                     VACSAVGLFGAARSSSFVQELPAPVPAKRPDDVADATMSRQRAPAFTRHFEMRWALGG
                     LPFSGDTEPRSMVYARLRDRECGAEDALLALADVIPTPVLSMLTQPAPASSLNWMLEV
                     LRDPAHLDLHGWCLLDTHVRAGTDGYLSQTSVLYGPDGHAYSVSHQTVGVFA"
     gene            complement(6745..7107)
                     /locus_tag="VD737_01965"
     CDS             complement(6745..7107)
                     /locus_tag="VD737_01965"
                     /inference="COORDINATES: protein motif:HMM:NF025569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPCC family cysteine-rich protein"
                     /protein_id="HXW09358.1"
                     /translation="MSQPKEPCPVCGYRVHERRCGSGASCPVCGWIDDFEQLVHPDLH
                     YGANPGLSLRQAQARLEALLVEEPGRASGLERDPRWRPLRAGETPVADPTGPSSPVCY
                     LGTPDPAGYVPYWRRPGR"
     gene            7268..8707
                     /locus_tag="VD737_01970"
     CDS             7268..8707
                     /locus_tag="VD737_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXW09359.1"
                     /translation="MTTSRRAFLCRSASLAGLAALPALRAWAADDSADVIVIGAGLSG
                     LMAAIELERAGHRVLVLEGRDRVGGKVLTFSGTQGVPEAGGNIIYGDYQRLIALAGRL
                     GVELEDQVPRLSRHAGYTLVLDGKPVSRREWPDSPRNPFPPALREMMPWQYVPTLTSQ
                     ENPLDSLDGWYAPENAPLDVSMRDFLRAQGATDDIIRLAYDTIPTYGMNARDVSALMM
                     AYVSAFTKLQKSARPVMLQARGGNQRLPEAMAKLLRNEIRFGQRVVAVRQEADRVTAH
                     TRDGGRYEAKAAICALPFATLREIDLDPGTTGVQAKAIRTLPHQAICQVALHATKPFW
                     EADGMEPSMWTDSHLGRVSAIYHGADADEVSSLVVSAFGPAAQHLDRLGAEGAARYVV
                     AEIERIRPAARGVLSVTGLHSWTRDPFAGGAWAYFHPGTVTRFLPEMFAPSGRVHFCG
                     EQTSVTSRGMEGALETGERAARAVARQLA"
     gene            complement(8766..9155)
                     /locus_tag="VD737_01975"
     CDS             complement(8766..9155)
                     /locus_tag="VD737_01975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HXW09360.1"
                     /translation="MKTSKFAPMFMIAAAACTLAGAGAYGQAMEVVTVEAVREIIVGK
                     SPIGAPIKELSIRSRVSYADLDLTTADGAAALEKRVKETAVSTCKELKVQIPAEGSTE
                     EKCVKEAMEGAAPQVAAAIEAAKKAKQ"
     gene            9511..>9866
                     /locus_tag="VD737_01980"
     CDS             9511..>9866
                     /locus_tag="VD737_01980"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HXW09361.1"
                     /translation="MHGNNLTSACLLCAALALSACQPSGREVSYARDVAPILELHCKG
                     CHMPGQAGYVVSGFELRNYETLMKGTQYGPVVLPGDPLTSVLVMLIEGRADPSLRMPH
                     GDAKPLDRADIETIRR"
BASE COUNT         1491 a         3435 c         3473 g         1467 t
ORIGIN      
        1 gcgacggctg ggaatcgcgc ggcgaggcga tcgcggtgta tggccgccgc aaccgcggct
       61 tcgagatgtc gcgggccatg acggcgtgga gcgacgacat cgtgctgtgc acgcacggcg
      121 cggccggtct gaacgccgag cagaaacgcc agctggcgcg caacggcgtg cggatcgtcg
      181 aggaaaagat cgagcggctc accggccgcg acgggcagct cgaggcgatc gtgttccgcg
      241 gtgggcgcag gctcgcacgg cgacgcctgt tcttcgacct gccctcgcac gcacagtcgg
      301 acctgtcgcg gcaactcggt tgccgcctcc gccgcaacgg cagcgtgcac tgcagcggct
      361 acgaggcgac cgacgtgcca ggcgtcttca tcgccggaaa catcgccggc gacgtgcagc
      421 tcgtgatctt cgcagccgcc gagggcgcga aggccgcgtt cggcatcaac cggtcgctga
      481 cccgcgagca gttcgagctg aacgcgacgg gccgccggat cgtcgagcac ccgaccaggg
      541 agtcgggctg aggctcagaa ggcgtagccg aactccagac cgccgtaggc gccgtcggcg
      601 tcgtcctgga aacggtagcc ggccttcagt ccgaacgtgt agcgcgcgtt gccgggacga
      661 aacgtcgtga cgaacccggc cgcggtcgca tccgcttcgt cgttgccgtt ggccaccgcc
      721 tcgaacccga aggaccgcgc ctgcgtcgtc ccgtgcatga gccttccgcg cagccagtag
      781 ccgttctgct cgttgctgta ggaagcgatg ccggcgatgc gccagtcctg cgcgaccgtg
      841 cgctcgaagt cgaactgcag cttgccgccg agctggctgc cccgcgcgtt cgctcgacgg
      901 tcgtcgggct ccagctgcgt gtccgtgtgg cgcaggccgg ccgagacgct ccaccagccg
      961 aggtcgctgg agccgccgta gccgagcgcc gcctcgacgc cgtaggcgtc ggcctcgacg
     1021 cgaccgggac cgccgtcgta ctcgtagccg aatgcgtcga cccagtagcg ctggtaccag
     1081 ccgcggccgt ccctgcgttc gccgaacggc aggatcgcgc cgacgtagct gtaataggcc
     1141 gcgtcggccg cctcggcgcc gaccagcagc agtcggtcgg cggcgactgc gcgcgggccg
     1201 gccagcatcg agcctgagcc gagcaccgcg acgaccaggg cgcggaaggt cgtcgaccag
     1261 gcgtcgcgga tgttcatgcg gtgagcttgt agaggtagat gggggccgcg tgcgtctcga
     1321 gcacggctac gtcctgcact cgtccgacca cgccgcgtgg cccggtggcc gtgacttcct
     1381 cggcgtacag gcggccgttc tgcggcagca tgcccatcga cacgaccgtg cgcacgcccg
     1441 tcctgggatc actgcgcgag atgaacccga cgccgtcgcc gtgctcggag ttgacgcgct
     1501 tgtagagcac cggcgcccag gtttcgccgg cgagccgccg cgactgctct gccagccccg
     1561 gccgcagcca gccccgcacg cgctcgagcg gcgtggccac gcggtccgtc aggccgttga
     1621 gctgccactg caggtcggag ccgaacagga acatctcgag ccggttgcgc tcgtccgaga
     1681 ctccccagcg gaactcgcag ccgagcgccg gttcgccggg gcgcggatcg tccgcgcccg
     1741 gcgcattgac cgcgcgcacc atcgcacgca cgggcagcgc tccggtggtc agcagcagtt
     1801 cgccgcgcag ttcctgcccg acgctggcag cgccggccgc cgcgccgacg acgcgacaac
     1861 cctgcggcga gatgtccgtg gcgagtgcca cgctttcgcg accggactcg tcctgcaacc
     1921 gcagcggcac cggcagcgga aaccggtact cacgccggcg cgaggacccg gactgcagcg
     1981 cgaagcgaat cgccgccacg gcgatgccgc aggtgagtgc cgcccagatc aggttcgcaa
     2041 taagtgcgcc ggccggcagt ccgcgttccg tgccgaacca cgcgatgccg accgggatcg
     2101 ccacgacgtt ggccgcgagc accaggtatt gcggccacag catccggcgc gtcgcgtcgc
     2161 cctcgcccat ctgcttcggc gtcaccacga accgcagctt gcgcaggaag aagccgaacg
     2221 tcgcggtgat gaagaccgcg aatcgcacca tgttgtactg ctcggtgagc acggtgcggc
     2281 cgtgaccgcg cgcgacttcc tcgaacgtcc agaacgtgag caggtagtac ggcaggaacc
     2341 ggaccaggaa ctcccggtcg agctgcagga tcggcatcgt gcccgtcgtc agcacgatca
     2401 cgggcgccgt gaagaagacg agccgctgcc agccttcgaa gtacgcgagc atcgtcgcga
     2461 ggtacgagag ccgctggcgc agcgtcaaac cgcgtgtgaa gagcacgcct tcctggcgcc
     2521 acacctgcat cgcgccctgc ccccagcgca ggcgctgctt caggaacggg atgatgctcg
     2581 cgggcgcgag tccgaacgcg agcgaacggc cgtagtagac cgacttccag ccgcgcttgt
     2641 gcaggcggat cgaggtgtgg atgtcttcgg tcacggtccc ggtcgagaac ccgccgatgt
     2701 cgtcaagggc cgtgcgccgg accaccgcgc agctgccgca gaagaacgcc gcgtcctggc
     2761 tgtccttgcc gggctggatc acgcgaaaga acagcgtctg ctcgctccag accagcgaat
     2821 cgcggcggtt cagccggtgc tggaacgagt cgaggttgta gaagtcctgc ggcgtctgca
     2881 cgaacgcgac cttcgggtcg ctgaagaacc cgagcgtctc gtcgaggaag ttgggcgccg
     2941 gcgcgtggtc cgcgtcgaag atcgcgacga attcggtgcg tgtgtgcccg agcgcgtggt
     3001 tgaggttgcc ggccttggcg tgcgtgttct cggtgcgtgc gaggtaccgg cagccgagtt
     3061 cgtcggcgag cacctgcatc tcgaggcggt tgccgtcgtc gagcagccag acctccgtgg
     3121 cgttccgcat gcgctgtgcc gccatgaccg tacggcggac gatttcgaca gactcgttga
     3181 tcgtcggcac gaacaccgcg acggtggcgc cctcgggcac cggcagcgac gtgcgctgtc
     3241 gcagtcgcca gcacatgccg aggtacagca ccgcgcacag gtatccgaac acctcggcgc
     3301 cgtagacgag cgcggagaac agcggcgcct ccgggttgaa cgcgctcggt cgccaggcca
     3361 ggtaccagag gccgactccc aggaacgtga acaccagcgc gcgttgcgcg agcgtgagtg
     3421 acgcgagcct gctgggcgga aacgcgactg cacccgagtt cgaaccgtcc gacacgtcgt
     3481 ctgacctctt gcaagcgctc gcgcgcggga gcgacgcgac tccacgcctg tgcggagcca
     3541 cgggcggcga ctgtaggcat cgccatggcg gcggaccgga cttaagtcgc aaagggcacg
     3601 agttatgtcg ccccggcgcg cacgacccgt gaccgccgtc accgttcgcg gaaagaagtc
     3661 cgctccggtt gcaattcggg tcgatcgggt tcccaattcc ggcgaggcgg tccggccccg
     3721 tggacccttg gacgtccggc atggaggctg ctgacgacaa cgacgaattc cccggccagc
     3781 cccacgaccg ccggcgcgaa cccgtgcgca tcgacccggc gctcggtcgc gcccaggagc
     3841 ggttcatgga gatgatcgcc tccggagccc cggtccggtc cgtgctcgag gcgatcgcgt
     3901 gcgaaatcca ggcgctcgtg ccgggtacgc aggtctgcgt gctggtcgtc gactcgacgg
     3961 ggacgtcgtt gcggcacggg gcggcaccga ccttgccccc cgagttcctg cgcgaggtcg
     4021 acggcctgcc gatcggaccg gccagcggcg cctgcgggac ggccgcgtat cgacgcgcgc
     4081 ccgtcgtcgt ggaggacatc gccacggacc cgctctggga cgacttccgc gcgcttgcgc
     4141 agcaacacgg gttgcgctcg gcgtggtcca gcccgatctg cgacgggcaa ggcggcgtgc
     4201 tcggcacgct ggctttctat cactgcgaac gctggacgcc gggatcctac gagcagagcg
     4261 tcgccgcgat ggcgacgcat gcggccgcgg tggcgttgcg tcgcgatcgc gaggagcagc
     4321 agctccgcaa gctctcggcg gccgtcgagc agagcgccga cagcatcgtg atcacggacc
     4381 tgcgcggcgt gatcgagtac gtcaacgaag cgttctgccg cgtgagcggc tactcgcgcg
     4441 acgaggtggt ggggtccacg ccggcgatcc tcaagtccgg gcgcacgccc gcggcgacgt
     4501 accgggagct ctgggagacg ctcgtccgcg gcgagtcgtg gcgcggcgag ttcgtgaacc
     4561 ggcgcaagga cggtcgcgag taccacgaga gcagcctgat caccccgttg cgccagccgg
     4621 acggccgcat cacgcattac ctggcggtca agtcggacgt cacggaacac aggcgcatct
     4681 ccgccgaact cgcgctgcac cggcaccacc tcgaggacct ggtcgagaag cgcacggcgg
     4741 aactcgcgct cgcgaaggta caggccgagt cggccaaccg cgccaagggc gtgttcctcg
     4801 cgaacatgag ccacgagatc cgcacgccga tgaactcgat cctcgggctc acctacatcc
     4861 tgcgccagca cacgagcgac cccgagcagc aggcgctgct cgcgcggctg cacgactccg
     4921 cgcagcacct gatgcacgtg atcaacggca tcctcgacct gtcgaagatc gaggccggca
     4981 agatcgcgat cgaggagtcc gagctctccg tcgagaacgt gctgcgcaac gtggtggagc
     5041 tgctgtcgca cgaagccgag gccaagaacc tcgtgctgga cgtcgaggtg cacggcgtcg
     5101 acggcgtgct ctacggcgat ccgacgcggc tgtcgcaggc actgctcaac tacgtctcga
     5161 acgcgatcaa gttcacgccg tcagggagaa tcctgctgcg cgcccgggtg gcgcgtgaag
     5221 acgcgaactc gcagtgcgtg cgcttcgaag tgcacgacac cggcccgggc atcgccgagg
     5281 accagctcgg caagctcttc aacgcgttcg agcaggggga cgcgtcaacc acgcgccggc
     5341 acggtggcac gggcctgggt ctcgcgatca cgcgcggcct cgcgcagatg atgggcggcg
     5401 acgcgggcgc cgagagcacg cacggcgccg gcagcacctt ctggttcacc gccacgctcg
     5461 gccggctcgc gccatcgacg atcacctccg agtcggcgct gcgagagttc cgcagctccc
     5521 agcggcccgc cgtcaagtcg gcggctcgcg agttctcggg acgcgtgctg ctcgccgagg
     5581 acaacccgac caaccagttc gtgctgctca ggctgctgcg cgacctgggc gtctcggtag
     5641 acctcgccga gaacggccgt gacgcggtgg agctgctgcg cgagcggccg tacgacctgg
     5701 tgctgatgga cgtgcagatg ccggagatgg acgggctggc cgccacacgc gccatccgcg
     5761 agctgccaca gcgtgagcag acgccgatcg tggcgctgac cgcgaacgcg ttcaccgacg
     5821 accgcgagcg ctgcctccag gcgggcatga acggtttcct gcccaagccg atcgacccga
     5881 cgctgctgcg cgaggcgctc gagcgctggc ttcgctaaaa tcgtcggatg accacggaat
     5941 tctcgcgcgt gctcgcgtcg atcgacgacg ggggcgacgg ttcgctgcgc gtcgcggtgc
     6001 caccggactg gctgcagggc cgtaccgtct tcggcggcat gcagatggcg atcgcggtgc
     6061 gcggcatgcg ctcctcgatg ccggagaccg cgcgcacgct gccgctgcgc tcggtgcaga
     6121 tgacgttcgt cggcccgctg acgggcagtg acccggttca gttgcgctcc gaactgctgc
     6181 gccagggccg ctcgatcacc cacgcccgct gcgacctgcg gcagtcgggc agcgtcgcct
     6241 gctcggcggt cggcctgttc ggcgccgctc gcagctcgtc gttcgtgcag gaactcccgg
     6301 cgccggtgcc ggccaagcgt cccgacgacg tcgccgacgc cacgatgtcg cgccagcggg
     6361 ctccggcgtt cacccggcac ttcgagatgc ggtgggcgct cggcgggctg ccgttcagcg
     6421 gcgacaccga accgcgttca atggtctacg cccgcctgcg ggaccgtgag tgcggggcgg
     6481 aagacgcgct gctcgcgctg gcggacgtga tcccgacgcc ggtgctgtcg atgctcacgc
     6541 agccggcgcc cgccagttcc ctgaactgga tgctcgaggt gctgcgcgat cctgcgcatc
     6601 tcgacctgca cggctggtgc ctgctcgaca cgcacgtccg tgccggcacc gacggttacc
     6661 tgagccagac gtctgtgctg tacggaccgg acggtcacgc gtactcggtg agccaccaga
     6721 cggtcggtgt gttcgcctga gccgctagcg ccccgggcgc cgccagtacg gcacgtaacc
     6781 ggccgggtcg ggcgttccga gatagcagac cggcgacgac ggcccggtcg ggtcggcgac
     6841 cggcgtctcg cccgcgcgga gcggccgcca gcgcgggtcg cgttcgagcc cgctcgcgcg
     6901 gccgggctcc tcgacgagca acgcctcgag gcgcgcctgc gcctggcgca ggctcaaccc
     6961 tggattggcg ccgtagtgca ggtcggggtg cacgagctgc tcgaagtcgt cgatccagcc
     7021 gcacaccggg cagctcgcgc cgctgccgca ccgtcgttcg tggacccggt acccgcagac
     7081 cgggcacggc tccttcggtt ggctcatgga cgcattatgc gcggccgttc gtgcgcgcgg
     7141 gcacggcggg gttgcgcgcg ccgccgccgc aggtagaaat acgcgcttgt ggcccccgtg
     7201 gagcgcccgg ttcaatccgc gctacacgcc tgcagacctc gcccggcgcc tgcgccgacg
     7261 gagatccatg acgacgtcac gtcgagcctt cctgtgccga tccgcgtccc tggccggcct
     7321 ggccgcgctg cccgcgctgc gcgcctgggc cgcagacgac tctgccgacg tgatcgtgat
     7381 cggcgccggt ctctccggcc tgatggcggc gatcgagctc gagcgcgccg gccaccgggt
     7441 cctggtgctg gagggtcgcg atcgggtggg cggcaaggtc ctgacgtttt cgggaacgca
     7501 gggcgtgccg gaggccggcg gcaacatcat ctacggggac taccagcggc tgatcgcgct
     7561 tgccggacgc ctgggcgtcg agctcgagga ccaggtaccc cggttgtcgc gtcacgccgg
     7621 ctacacgctc gtgctcgacg gcaagccggt ctcgcgcagg gagtggccgg attcaccgcg
     7681 caacccgttt ccgccggcgc tgcgcgagat gatgccctgg cagtacgtgc cgacgctgac
     7741 gagccaggag aacccgctcg actcgctcga cggctggtac gcgccggaga atgcaccgct
     7801 cgacgtgtcg atgcgcgatt tcctgcgcgc gcagggggcg accgacgaca tcatccgcct
     7861 cgcctacgac accattccga cctacggcat gaatgcgcgc gacgtgtccg cgctgatgat
     7921 ggcctacgtc tcggcattca cgaagctgca gaagagcgcc cgtccggtga tgctgcaggc
     7981 gcgcggcggc aaccagcgcc tgccggaagc catggcgaag ctgttgcgca acgagatccg
     8041 gttcggccag cgggtggtgg ccgtgcggca ggaagccgac cgggtcaccg cgcacaccag
     8101 ggacggcggc cgctacgagg cgaaggccgc gatctgcgcc ctgccgttcg cgacgctgcg
     8161 cgaaatcgac ctggatcccg gcacgaccgg cgtgcaggcc aaggccatcc gcacgctgcc
     8221 tcaccaggcc atctgccagg tggcgctgca tgccacgaag ccgttctggg aagccgacgg
     8281 catggagccg tcgatgtgga cggactcaca cctcggccgc gtctcggcga tctaccacgg
     8341 cgccgacgcg gacgaggtct cgagcctggt ggtctccgcg tttggaccgg cggcacagca
     8401 cctcgaccgg ctgggcgccg agggcgcggc gcggtacgtc gtggccgaga tcgagcgcat
     8461 ccgcccggcg gcgcggggcg tgctgagcgt cacggggttg cactcgtgga cgcgcgaccc
     8521 gttcgccggc ggcgcctggg cgtacttcca cccgggcacg gttacgcgtt tcctgcccga
     8581 gatgttcgcc ccgagcggcc gcgtgcactt ctgcggcgag cagacgtcgg tgacgtcgcg
     8641 gggcatggag ggcgcgctgg agaccgggga acgcgcggcg cgcgcggtgg cccgccagct
     8701 cgcctagggc cgccgccagg atcgggcagg cggccggagc cgcccgccct gaccgtcctg
     8761 gacgattact gcttggcctt cttcgcggct tcgatggccg cggcgacctg cggggccgcg
     8821 ccttccatcg cttccttgac gcacttttcc tcggtcgagc cttcggccgg gatctgcacc
     8881 ttgagttcct tgcaggtcga caccgcggtc tccttgaccc gcttctcgag cgcggcggca
     8941 ccgtcggccg tcgtcaggtc gaggtccgcg tagctcacgc gcgagcggat gctgagttcc
     9001 ttgatcggtg cgccgatcgg cgacttgccg acgatgattt cacgcacggc ctcgaccgtg
     9061 acgacttcca tggcctggcc atacgcgccg gcgcctgcca gcgtgcaggc cgcggctgca
     9121 atcatgaaca tcggggcaaa cttgctggtc ttcatcgcgg agatctccgg ttgttgcggt
     9181 gctgtgcagt cgtagggtcc ggagtcgggc agcaacgtcg ttcgtgcggt gtcgtccgga
     9241 cgtcgctgca gagaatagac gttcggcgcg aacacggtca ttgcggtcgt tacggactcg
     9301 cgattcgcag acggccaggg ccccaggcgc acggcttgct tacatggacc cggtgccttt
     9361 cgtggcagaa cgcacctgcc aggccgcggg gcgccgcggg accggcggct ggcggcgcct
     9421 gtacactcgt gctaggtaga tcagcggatt gtggcgatcg ccttcccgcc ggatgttctg
     9481 cctcaggacc cgtctgcatg cggaggcttt atgcacggaa acaacctgac gtccgcctgc
     9541 ctcctgtgcg ctgcgctggc gctgagcgcc tgccagccgt ccgggcgcga agtgagctat
     9601 gcacgcgacg tggctccgat cctcgagctt cactgcaagg gttgccacat gcccggccag
     9661 gccgggtacg tcgtgagcgg tttcgagctg cgcaactacg agacgctcat gaagggcacg
     9721 cagtacggcc cggtcgtgct gccgggtgat ccgctcacga gcgtgctggt catgctgatc
     9781 gagggccgcg cagacccgtc gctcaggatg ccgcacggcg acgcgaagcc gctcgatcgc
     9841 gccgacatcg agacgatccg gcgctg
//
LOCUS       DASPFR010000082         8849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1613262, whole genome shotgun sequence.
ACCESSION   DASPFR010000082 DASPFR010000000
VERSION     DASPFR010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8849
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613262"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..479)
                     /gene="rplR"
                     /locus_tag="VD737_01985"
     CDS             complement(123..479)
                     /gene="rplR"
                     /locus_tag="VD737_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003463284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HXW09362.1"
                     /translation="MSTKNVARLRRARQTRIRIREAGAVRLTVYRTNAHIYAQITSPS
                     GDKVLATASTLEKDVRGQLKHGGNRKAAELVGQRIAEKAKQAGIAAVAFDRAGYRYHG
                     RVKALAEAARAGGLKF"
     gene            complement(519..1052)
                     /gene="rplF"
                     /locus_tag="VD737_01990"
     CDS             complement(519..1052)
                     /gene="rplF"
                     /locus_tag="VD737_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HXW09363.1"
                     /translation="MSRIAKYPVALPKGVDVTIAGGNISVKGPLGTVARATDPNVAVE
                     KKGETLEFKALGNSNHADAMSGTMRALVAGMVTGVTKGFEKRLALVGVGFRAQAQGDK
                     LSLSLGYSHPIVHQMPKGIKVATPTQTEIVISGIDKQLVGQVAAEVRAYRPPEPYKGK
                     GVRHRDEVVELKETKKK"
     gene            complement(1057..1464)
                     /gene="rpsH"
                     /locus_tag="VD737_01995"
     CDS             complement(1057..1464)
                     /gene="rpsH"
                     /locus_tag="VD737_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HXW09364.1"
                     /translation="MSMSDPIADMLIRIRNAQLVGHASVDMPASKLKAAIAQVLKDEG
                     YIEDFAVRDKGGDDGAKKELRIGLKYYAGRPVIERLERVSKPGLRVYRGRNDIPRIMN
                     GLGVAILSTSRGVMTDRKARADGVGGEVLCIVA"
     gene            complement(1508..1813)
                     /gene="rpsN"
                     /locus_tag="VD737_02000"
     CDS             complement(1508..1813)
                     /gene="rpsN"
                     /locus_tag="VD737_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HXW09365.1"
                     /translation="MAKLAVKLRDQKRRKTVEKFKAKRAQLLEILHDARASDEAKQEA
                     REKLQRLPRDASPVRLRNRCALTGRPRGVFRKFGLARNKLRELALRGEVPGVIKASW"
     gene            complement(1825..2349)
                     /gene="rplE"
                     /locus_tag="VD737_02005"
     CDS             complement(1825..2349)
                     /gene="rplE"
                     /locus_tag="VD737_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HXW09366.1"
                     /translation="MLYREKVVPDLMQKHGYKTLMQVPRIRKITVNMGVGETTTDKKV
                     LDAAVADMAKITGQKPVVTKSRKSIANFKIRANFPVGCMVTLRGARMYEFLDRLVNVA
                     MPRIRDFRGVSNRAFDGRGNYNLGFKEQIIFPEIDYDKIDMLRGMNIAITTTAKTDDE
                     ARSLLAAFRFPFKH"
     gene            complement(2529..2849)
                     /gene="rplX"
                     /locus_tag="VD737_02010"
     CDS             complement(2529..2849)
                     /gene="rplX"
                     /locus_tag="VD737_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HXW09367.1"
                     /translation="MMNRIRKGDEVVVLAGRDKGKRGTVLRRVDAEHVVVEGVNRVKK
                     NQRPNPMKGVSGGIVDKDLPIHVSNVAVFNPATKKGDRISMKTLPDGRKVRIFKANDE
                     QVDA"
     gene            complement(2846..3214)
                     /gene="rplN"
                     /locus_tag="VD737_02015"
     CDS             complement(2846..3214)
                     /gene="rplN"
                     /locus_tag="VD737_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HXW09368.1"
                     /translation="MIQMQTVLDVADNTGARAVMCIKVMGGSKRRYASIGDLIKVSVK
                     DAAPRGRVKKGEIYDAVVVRTAKGVRRSDGSLIRFDRNAAVLLTPKMEPIGTRIFGPV
                     TRELRTEQFMKIVSLAPEVL"
     gene            complement(3286..3540)
                     /gene="rpsQ"
                     /locus_tag="VD737_02020"
     CDS             complement(3286..3540)
                     /gene="rpsQ"
                     /locus_tag="VD737_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HXW09369.1"
                     /translation="MSNQRALVGRVTSNKMQKTVVVRVERRVRDALMGKTITRSRKYH
                     AHVEEGQFGEGDLVEIAECRPMSRTKSWRVTRLVEKSKAV"
     gene            complement(3537..3728)
                     /gene="rpmC"
                     /locus_tag="VD737_02025"
     CDS             complement(3537..3728)
                     /gene="rpmC"
                     /locus_tag="VD737_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002555481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HXW09370.1"
                     /translation="MKASELRGKTGDELQKELEGLLRARFGLRMQLATQQLSNTSQLK
                     KVRKDIARVRTIMKEKAAK"
     gene            complement(3731..4147)
                     /gene="rplP"
                     /locus_tag="VD737_02030"
     CDS             complement(3731..4147)
                     /gene="rplP"
                     /locus_tag="VD737_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009115861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HXW09371.1"
                     /translation="MLQPARTKYRKQQKGRNTGNATRGAKVSFGEYGLKAATRGRLTA
                     RQLEAGRRAMSRHIKRGGRIWIRVFPDKPVSQKPAEVRMGNGKGNPEYFVCEIQPGKI
                     IFEMDGVTEALAKEAFALASAKLPFRTIIVQRMLGQ"
     gene            complement(4402..5028)
                     /gene="rpsC"
                     /locus_tag="VD737_02035"
                     /pseudo
     CDS             complement(4402..5028)
                     /gene="rpsC"
                     /locus_tag="VD737_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003176422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            complement(5028..5357)
                     /gene="rplV"
                     /locus_tag="VD737_02040"
     CDS             complement(5028..5357)
                     /gene="rplV"
                     /locus_tag="VD737_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018420150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HXW09372.1"
                     /translation="METRASLRGARLSAQKGRLVADLIRGKPVDQAINILAYTPKKGA
                     VLIRKLLESAIANAEHNDGADIDTLKVKSITVERGMFLKRFQARAKGRGNRILKHTCH
                     IYITVGD"
     gene            complement(5484..5753)
                     /gene="rpsS"
                     /locus_tag="VD737_02045"
                     /pseudo
     CDS             complement(5484..5753)
                     /gene="rpsS"
                     /locus_tag="VD737_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
     gene            complement(5767..6594)
                     /gene="rplB"
                     /locus_tag="VD737_02050"
     CDS             complement(5767..6594)
                     /gene="rplB"
                     /locus_tag="VD737_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HXW09373.1"
                     /translation="MPLVKVKPTSPGRRALVKVVTPELHKGEPFWPLTRKQSKTVGRN
                     HHGTITMRHKGGGNKQHYRLVDFRRDKDGINARVERLEYDPNRSAHLALLLYADGERR
                     YMIAPKGLQVGAQVVSGAEAPIKTGNALPLRNVPVGTIVHCVEMLPGKGAQLARSAGT
                     GVQVLAREGSYAQLRLRSGEIRKVHIDCRATIGEAGNAEHNLESIGKAGRKRWRGVRP
                     TVRGVAMNPIDHPHGGGEGRTAAAGPPQSPWGTKSKGFKTRRNKRTQAMIVRDRRVK"
     gene            complement(6600..6878)
                     /gene="rplW"
                     /locus_tag="VD737_02055"
     CDS             complement(6600..6878)
                     /gene="rplW"
                     /locus_tag="VD737_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008343846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HXW09374.1"
                     /translation="MNVVLAPVVSEKSTFVAERNRQYVFRVADGATKPEIKAAVELMF
                     KTKVDSVTVSNVKGKERRFGRTIGRKRNWKKAYVRLAEGQEINFAATE"
     gene            complement(6902..7525)
                     /gene="rplD"
                     /locus_tag="VD737_02060"
     CDS             complement(6902..7525)
                     /gene="rplD"
                     /locus_tag="VD737_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HXW09375.1"
                     /translation="MELKMVAEKGTSGTGTFNAKDEVFARDFNEALVHQLVVAYQANA
                     RIGSRAQKDRGAVNHSTKKPWRQKGTGRARAGMTSSPLWRGGGRIFPNSPDENFSHKV
                     NRKMYRAGMASILSQLAREDRIRVVDEFKVDAPKTKLLAQKVKAMGFDEVLVLTEEVD
                     QNLRLSSRNLENVQVMSVRNANPVALVRCAKVLLTMGAVARLEELLK"
     gene            complement(7635..8270)
                     /gene="rplC"
                     /locus_tag="VD737_02065"
                     /pseudo
     CDS             complement(7635..8270)
                     /gene="rplC"
                     /locus_tag="VD737_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005350169.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
     gene            complement(8373..8684)
                     /gene="rpsJ"
                     /locus_tag="VD737_02070"
     CDS             complement(8373..8684)
                     /gene="rpsJ"
                     /locus_tag="VD737_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HXW09376.1"
                     /translation="MQSQRIRIRLKAFDYRLIDQSALEIVDTAKRTGAVVRGPVPLPT
                     RKQRFDLLRSPHIDKASRDQLEIRTHLRLMDIIDPTDKTVDALMKLDLPAGVDVEIKV
                     Q"
     gene            complement(8687..>8849)
                     /gene="tuf"
                     /locus_tag="VD737_02075"
     CDS             complement(8687..>8849)
                     /gene="tuf"
                     /locus_tag="VD737_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117403.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HXW09377.1"
                     /translation="VELPKGTEMVMPGDNVKMVVTLITPIAMEQGLRFAIREGGKTVG
                     AGVVAKIIE"
BASE COUNT         1152 a         3037 c         2811 g         1849 t
ORIGIN      
        1 ccgagcacgc ggccgccctt caccaccttg gtgacgcggt tgaccgcgac catcttctcg
       61 cgcaggccgt cgccgcgctc ctcgtgctgc atcctgggtt gaatcttcgc catgagccct
      121 cgctagaact tcaatccgcc tgcacgtgcc gcctcggcga gcgccttcac gcgcccgtga
      181 tacctgtagc cggcgcgatc gaacgcgacc gccgcgatgc ccgcctgctt cgccttctcg
      241 gcgatgcgct ggccgacgag ctccgccgcc ttgcggttgc cgccgtgctt gagctgcccg
      301 cgcacgtcct tctccagcgt ggacgcggtg gcgagcacct tgtcgcccga aggcgaggtg
      361 atctgcgcgt agatgtgcgc gttggtgcgg tagacggtca gccgcacggc gccggcctcg
      421 cggatccgga tccgggtctg gcgtgcgcgg cgcagacggg caacattttt cgtactcatc
      481 gagtctccat cgccttcatt tcgacgcctt catttcaact atttcttctt cgtctccttc
      541 agctcgacca cttcatcgcg atggcgcacg cccttgccct tgtagggctc gggggggcgg
      601 taggcgcgga cctccgcggc gacctggccg acgagctgct tgtcgatgcc gctgatcacg
      661 atctcggtct gcgtcggcgt cgcgactttg atgcccttcg gcatctggtg cacgatcggg
      721 tgcgagtatc cgagcgaaag cgagagcttg tcgccttgcg cctgggcgcg gaagccgacg
      781 ccgacgagcg cgaggcgctt ctcgaacccc ttggtgacgc cggtcaccat gccggcgacc
      841 agcgcccgca tcgtgcccga catcgcgtcg gcatggttcg aattgccgag cgccttgaac
      901 tcgagcgtct cgcccttctt ctccaccgcg acattgggat cggtcgcgcg cgcgacggtg
      961 ccgagcggtc ctttgacgga aatgttgccg ccggcgatgg tgacgtccac gcccttgggc
     1021 aacgcgaccg ggtatttggc gattctggac atgcgcctag gccacgatgc aaagcacttc
     1081 gccgcccacg ccatcggcgc gggccttgcg gtcggtcatg acgccgcgcg aggtggacag
     1141 gatggcgacg ccgagcccgt tcatgatgcg cggaatgtcg ttgcggccgc ggtagacccg
     1201 cagcccgggc ttggatacgc gctccagccg ctcgatcacc gggcgtccgg cgtagtactt
     1261 gagcccgatg cgcagctcct tctttgcgcc gtcgtcgccg cctttgtcgc ggacggcgaa
     1321 gtcctcgatg tagccctcgt ccttcagcac ctgggcgatc gccgccttca gcttcgaggc
     1381 gggcatatcg acgctcgcgt gcccgaccag ctgggcgttg cgaatccgga tcagcatgtc
     1441 cgcgatcggg tcgctcatgc tcatgcgcat ctcctgcctt cattggcctt catgcctacc
     1501 ctcatggcta ccagctcgcc ttgatcacgc ccggcacctc gccgcgcagc gccagctcgc
     1561 gcagtttgtt gcgcgccagc ccgaacttgc ggaacacccc gcgcggccgg ccggtaagcg
     1621 cgcagcggtt gcgcagccgc accggcgagg cgtcgcgcgg cagcctctgc agcttttcgc
     1681 gcgcctcctg cttcgcctcg tcggaggcgc gggcgtcgtg caggatctcg agcagctgcg
     1741 cgcgcttcgc cttgaacttc tccaccgtct tgcggcgctt ctgatcgcgg agcttgactg
     1801 ccagcttggc catgcggttc tctctcagtg cttgaacggg aagcggaacg cggcgagcag
     1861 cgagcgcgcc tcgtcatcgg tcttcgccgt ggtggtgatg gcgatgttca ttccccgcag
     1921 catgtcgatc ttgtcgtagt cgatctcggg gaagatgatc tgttccttga agccgaggtt
     1981 gtagttgccg cggccgtcga aagcgcgatt cgatacgccg cggaagtcgc ggatgcgggg
     2041 catcgccacg ttcaccagcc ggtcgaggaa ctcgtacatg cgcgcgccgc gcagcgtcac
     2101 catgcagccg accggaaagt tggcgcggat cttgaagttg gcgatcgact tgcgcgactt
     2161 ggtcaccacc ggcttctgtc cggtgatctt cgccatgtcg gcgaccgcgg cgtcgagcac
     2221 cttcttgtcg gtcgtggtct cgcccacgcc catgttgacg gtgatcttgc ggatgcgcgg
     2281 cacctgcatc agggtcttgt agccgtgctt ttgcatcagg tcgggcacga ccttctcgcg
     2341 gtagagcagg gcgaggcgcg ccgggggtgg aggaagctcg ggcgcggcgg gcttggccgg
     2401 cgcggcctcc ttcttggcct gctgcgcttc cttcttcggc ttttgcgcct gctgctgagg
     2461 cgccggcgcc tgcttcttgt ccgaccgttc ggtcttgtct tgcttgtcct gcttctcttg
     2521 cttcgccatt acgcgtccac ctgctcgtcg ttcgccttga agatgcgcac tttgcgcccg
     2581 tccggcaggg tcttcatcga gatgcggtcg cccttcttgg ttgccgggtt gaacaccgcc
     2641 acgttggaga cgtggatcgg caggtccttg tcgacgatgc cgccgctcac ccccttcatc
     2701 gggttggggc gctggttctt cttcacgcgg ttgacgccct cgaccaccac gtgctcggca
     2761 tcgacgcgcc gcagcacggt gccgcgcttg cctttgtcgc gtccggcgag caccaccact
     2821 tcgtcgcctt tccggatgcg gttcatcaca ggacctcggg cgcgagcgat acgatcttca
     2881 tgaactgctc ggtgcgcaac tcgcgcgtca ccggtccgaa aatgcgcgtg ccgatcggct
     2941 ccatcttcgg ggtgagcagc accgcggcgt tgcggtcgaa acggatcagc gagccgtcgg
     3001 agcggcgcac gcccttggcg gtgcgcacga ccaccgcgtc gtagatctcg cccttcttca
     3061 cccggccgcg cggcgccgcg tccttcacgc tcaccttgat caggtcgccg atcgaggcgt
     3121 agcgccgctt cgagccgccc atcaccttga tgcacatgac ggcgcgcgcg ccggtgttgt
     3181 cggcgacgtc caataccgtt tgcatctgga tcatctaagt ttttctccgc tccaacttgc
     3241 accgctgcgc ggcgcagtct tggaccccgt cgggggaccc gaattctaga cggccttcga
     3301 cttctccaca aggcgcgtga cacgccagga cttggtcctg gacatcggcc ggcattccgc
     3361 gatctcgacg agatcgcctt cgccgaactg accttcctcg acatgcgcgt ggtatttccg
     3421 cgagcgcgtg atggtcttgc ccatcagggc atcgcgcaca cgccgctcga cgcgcaccac
     3481 gaccgtcttc tgcatcttgt tgctcgtcac ccgcccgacc agggcccttt gattgctcat
     3541 ttcgccgcct tttccttcat gatggtccgg acccgcgcga tgtccttgcg caccttcttc
     3601 aactggctgg tgttcgaaag ctgctgggtc gcgagctgca tgcgcaggcc gaaccgggcg
     3661 cgcagcaggc cctccagctc cttttgcagc tcatcgcccg tcttgccccg gagttcgctc
     3721 gctttcatgg ctactgtccc agcatgcgct gcacgatgat ggtgcggaac ggcagcttgg
     3781 cagaggcgag cgcgaacgcc tctttcgcca gcgcctcggt gacgccgtcc atctcgaaga
     3841 tgatcttgcc gggctggatc tcgcacacga agtactcggg gttgcccttg ccgttgccca
     3901 tgcgcacttc cgccggcttc tgcgacacgg gcttgtccgg aaacacgcgg atccagatcc
     3961 ggccgccgcg cttgatgtgg cgcgacatgg cgcggcgccc ggcctcgagc tggcgcgcgg
     4021 tcagccggcc gcgcgtggcg gccttgaggc cgtactcgcc gaacgacacc ttggccccgc
     4081 gcgtagcgtt gccggtattt cggcctttct gctgcttgcg gtacttggtg cgtgcaggtt
     4141 gcaacatgct cagttacctt tctcttcctt tttctcgcct tcttccttca cgatcttctt
     4201 gggttttttc acgatcgttt tcggcgccgc ggcttccggc ttcttcgcct tcttctcgcc
     4261 ctcggggcgg ggtttcgccg gcccgcgcct ggcgccggct ttcgggaagg gcttcttggc
     4321 gcgcttgggc tccgcgggcg cctcgggctg cggcagcggt gccggcgccg gcgccggcag
     4381 cagcgcggtc tcgcctttcg gtatcacttc gcccttgtac acccagacct tgatgccgat
     4441 cacgccgtag ttggtcttcg cttcgcccag gccgtagtcg atgtcggcgc gcagcgtgtg
     4501 cagcggcaca cgcccttcgc ggtaccactc ggtgcgcgcg atctcgatgc cgttcaggcg
     4561 gcccgcgctc atgatcttga tcccctgcgc gcccaggcgc atggcgttct gcatggcgcg
     4621 cttcatcgcc cggcggaaca tgatgcgctt ctcgagctgc tgagcgatcg agtcggcgat
     4681 cagctgcgcg tcgatctcgg gcttgcggat ctcctcgatg ttcacgtgta cctgctgcac
     4741 gcccatgatg cggcgcagct ccgccttgag cgcctcgatc tcctcgcctt tcttgccgat
     4801 caccacgccc ggacgcgccg aaaaaatggt gatgcgcgca tccttcgcgg gacgctcgat
     4861 cagcacgcgc ccgacggaag cgtgcgccag cttcttcttc aggaagtcgc gcaccttgat
     4921 gtcctcggcg agcatcggag cgaacgagcg gctgttcgca taccagcgcg agctccagtt
     4981 gcggttgacc gacagcctga agcccaccgg gttgatcttt tgacccatca gtcacccacc
     5041 gtaatgtaga tgtggcacgt gtgtttcagt atccggttcc cgcgtccctt ggcgcgtgcc
     5101 tggaagcgct tgaggaacat gccgcgctcg accgtgatcg acttgacctt gagggtatcg
     5161 atgtcggcgc cgtcgttgtg ctcggcgttg gcgatcgccg actcgaggag cttgcggatc
     5221 agcaccgctc ccttcttcgg cgtgtaggca aggatgttga tcgcctggtc gaccggcttg
     5281 ccgcggatca ggtcggccac cagccggcct ttctgggccg agagccgagc gccgcgcagg
     5341 ctcgctctgg tttccatcta tttcttcccg cctgcagggg ccgccggcgc ggccgccggg
     5401 gccgccgccg gcgcggccgc gggcgtcgcg gcgacctgcg cgcccggcgg cagccggcgg
     5461 cgctcctcgc cgaccgcttc ctcggtgcgc tttccgaccg ccgagtggct cttgaagatg
     5521 cgcgtcagcg cgaactcgcc gagcttgtgg ccaaccatgt tctcggtcac gtacaccggc
     5581 atgtgcgtct tcccgttgtg cacggcgatc gtcaggccga cgaactccgg cacgatggtc
     5641 gagcgccgcg accaggtctt gatcgggcgc ttgtcgttgc cggcgcgggc tttgtcaacc
     5701 ttcgccatca ggtgatggtc cacaaaggga cctttcttga ttgagcgtga catggccttg
     5761 tttcccttat ttcacccggc ggtcgcggac gatcatcgcc tgcgtgcgct tgttgcggcg
     5821 cgtcttgaat cccttgctct tcgtgcccca gggcgactgg ggcgggccgg ccgccgcggt
     5881 gcggccttcg ccgccgccgt gcggatggtc gatcgggttc atggctacgc cgcgcaccgt
     5941 cgggcgcacg ccacgccagc gcttgcggcc cgccttgccg atcgactcga ggttgtgctc
     6001 cgcgttgccc gcctcgccga tggtggcgcg gcagtcgatg tgcaccttgc ggatctcgcc
     6061 cgagcgcagc ctgagctgcg cgtacgagcc ctcgcgcgcg agcacctgca cgcccgtgcc
     6121 ggccgagcgc gcgagctggg cgcccttgcc cggcagcatt tccacgcaat gcacgatggt
     6181 gccgaccgga acgttgcgca gcggcagcgc gttgccggtc ttgatcgggg cctcggcgcc
     6241 ggacacgacc tgggcgccga cctgcaggcc cttcggcgcg atcatgtagc ggcgctcgcc
     6301 gtcggcatag agcagcagcg cgaggtgcgc gctgcggttc ggatcgtatt cgagccgctc
     6361 gacgcgggcg ttgatgccgt ccttgtcgcg ccggaaatcg actaagcggt agtgctgctt
     6421 gttgccgccg cccttgtggc gcatggtgat cgtgccatgg tggttgcggc cgaccgtctt
     6481 cgactgcttg cgcgtgagcg gccagaacgg ctcgcccttg tgcagctcgg gcgtgactac
     6541 cttgacgagg gcgcggcggc cgggcgaagt gggtttgact ttgacgagag gcatgggtct
     6601 tactccgtgg cggcgaagtt gatttcctga ccctcggcga gacgtacgta ggccttcttc
     6661 cagttgcgct tgcgcccgat ggtacggccg aagcgccgct ccttgccctt gacgttcgac
     6721 acggtgaccg agtcgacctt ggtcttgaac atgagctcga ccgccgcctt gatctcgggc
     6781 ttggtcgcgc cgtcggcgac gcggaacacg tactggcggt tccgttcggc gacgaaggtg
     6841 ctcttctccg acaccaccgg cgcgagcacc acgttcatca gctggtcgat gttgtacttt
     6901 ttcacttgag cagctcctcg agccgggcca ccgcgcccat ggtcagcagc actttcgcgc
     6961 agcgcaccag tgccaccggg ttcgcgttgc gcaccgacat cacctgcaca ttctcgaggt
     7021 tgcgcgacga cagccgcagg ttctggtcca cctcctcggt gagcaccagc acctcgtcga
     7081 aacccatcgc cttcaccttc tgggcgagca gcttggtctt gggcgcgtcg accttgaatt
     7141 cgtccaccac gcggatgcgg tcctcgcgcg cgagctgcga caggatcgac gccatcccgg
     7201 cgcggtacat cttgcggttc accttgtgcg agaaattctc atcgggcgag ttcggaaaga
     7261 tcctgccgcc gccgcgccac agcgggctgg aggtcatgcc ggcgcgcgcg cgcccggtgc
     7321 ccttctgccg ccagggcttc ttggtggagt ggttcaccgc gccgcggtcc ttctgcgcgc
     7381 gcgatccgat gcgcgcgttc gcctggtagg cgacgacgag ctggtggacc agcgcctcgt
     7441 tgaaatcgcg cgcgaacact tcgtccttgg cgttgaaggt gccggtgccg cttgtgccct
     7501 tctcggcgac catcttgagt tccatgtcag gccttcggcg cgggttgctt ggcggcggcg
     7561 ggcttcgcct cggccttcgg ctctgccttg ggtgcggctt tcggcgcctg cgccttgacc
     7621 ggtgcccttt gccccttcgc ggcgaggcgc acgacgacat cgcgcccgct cgagcccggc
     7681 accgatccct tcacgagcag cagctgcctt tcgggatcga tgcgtacgat ctccaggttg
     7741 gagatggtgc gccgcacgtc accgaggtgc ccgggcatgc gcttgccggg gaagacgcgc
     7801 gccgggctct ggttctggcc ggtcgagccg ggggtcttgt gcgagatgga gttgccgtgg
     7861 ctcgcgcgct gcgagtggaa atggtggcgc ttgatgacgc cggcgaaccc cttgccgatg
     7921 gtcacgccct gcacgtcgac cagttgcccg accttgaaca gctcctgcac cgcgagcttc
     7981 gccccgggct tgagagccga aacgtcattt tcctctgttc taaattcctt cagcacatgg
     8041 ccggcttcga cggcggcctt ggcgaggtgg ccggcgagcg gtttgttgac gcgcgtggcg
     8101 cggcgcttgc caaacgccac ctgcacggcg gcgtagccgt ccttctccgc ggtcttgact
     8161 tgcgtcaccc ggttgtcgga gacgtcgagc accgtgaccg gcagcgtcac gccgtcctcg
     8221 gcgaagattc gcatcatgcc gaccttgcgc ccgaccaatc caatcgtcat acgtgcctcg
     8281 tctgtatgga tccccgcttg cgcggggacg ccggctgcag ttggccggca ctgcaccttg
     8341 catttcagcg gcgtttcttg ccgctccttc tactactgaa ccttaatctc cacgtcgact
     8401 ccggcgggta gatcgagctt catgagcgcg tctaccgtct tgtcggtcgg gtcgatgatg
     8461 tccatcagcc tcaaatgcgt gcggatctcg agctggtcgc gcgaagcctt gtcgatgtgc
     8521 ggcgagcgca gcagatcgaa gcgctgcttg cgcgtcggca gcggcaccgg gccgcgcacc
     8581 acggcgcccg tgcgcttcgc cgtatccacg atctccagcg ccgactgatc gatcagccga
     8641 tagtcgaagg ctttgagccg aattctgatt ctttggcttt gcatttctac tcaataatct
     8701 ttgccacgac gcccgcgccg acggtcttgc cgccctcgcg gatcgcgaag cgcagcccct
     8761 gctccatcgc gatcggcgtg atcagcgtca ccaccatctt cacgttgtcc cccggcatca
     8821 ccatctcggt gcccttcggc agctccacc
//
LOCUS       DASPFR010000083         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1247130, whole genome shotgun sequence.
ACCESSION   DASPFR010000083 DASPFR010000000
VERSION     DASPFR010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247130"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VD737_02080"
     CDS             complement(<1..999)
                     /locus_tag="VD737_02080"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXW09378.1"
                     /translation="MLDAELKQQLQDYLARMTQPIELVASLDDSETAREMAALLQDIA
                     TLSPLVGYRELRGADVAAGTRAPSFRITRVGHDMGITFAGLPMGHEFTSLVLALLQAG
                     GYPPKVDRAVIEQIEALDGDFEFEIFISLSCHNCPEVVQSLNLMAVLNPRVRVTTIDG
                     ALFQDEVERRQVMAVPFVFLNGKEFNQGRISLEELLAKVDTRAGERAAAQIDAKGTFD
                     VLVVGGGPAGASAAIYAARKGIRTGVVADRFGGQVMDTLGIENFISVKATDGPRLAAA
                     LEEHVKHYDVDIMNLQRATELEPAGADGLVSVKLASGASVQSRTVVIATGARWRKMN"
     gene            complement(1123..1686)
                     /gene="ahpC"
                     /locus_tag="VD737_02085"
     CDS             complement(1123..1686)
                     /gene="ahpC"
                     /locus_tag="VD737_02085"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HXW09379.1"
                     /translation="MNLINTTLKPFKTTAFHNGKFVEVTDQTVRGKWSVFIFMPAAFT
                     FNCPTEVEDAADNYTEFQKLGAEVYIVTTDTHFSHKVWHETSPAVGKAKFPLVGDPTH
                     QLTRAFGVHIEEEGLALRGTFVLNPDGVIKTLEIHDNSIARDVKETLRKLKAAQYVAA
                     NPGEVCPAKWNDGSRTLKPSLQLVGKI"
     gene            2012..>2372
                     /locus_tag="VD737_02090"
     CDS             2012..>2372
                     /locus_tag="VD737_02090"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXW09380.1"
                     /translation="MAHLDAYLFFDGTCADAMRFYERVFHGRLEVLQTYAESPAAHDV
                     APDRAHRIMHARLVIDGRALLASDAVSEEPFEGTRGFALSMNYDTVDEAQRVFVELGD
                     GGTVQMPLQQTFWAQAFG"
BASE COUNT          365 a          797 c          796 g          414 t
ORIGIN      
        1 gttcatcttc cgccagcgcg cgcccgtggc aatgacgacg gtgcggctct gcacgctcgc
       61 gccgcttgcg agcttgacgg aaaccaggcc gtccgcgccc gccggctcga gctccgtggc
      121 ccgctgcagg ttcatgatgt cgacgtcgta gtgcttgacg tgctcctcga gcgccgcagc
      181 gagccgcggg ccgtcggtcg ccttgaccga gatgaagttc tcgatgccga gcgtgtccat
      241 cacctgcccg ccgaaacggt cggccacgac gcccgtgcgg atgcccttgc gcgccgcata
      301 gatggcggcg gacgcgcccg ccggaccgcc gcccacgacc agcacgtcga acgtaccctt
      361 cgcgtcgatc tgcgcggccg cgcgctcacc ggcgcgcgtg tcgaccttgg ccagcagctc
      421 ctcgaggctg atgcggccct ggttgaattc cttgccgttc aggaacacga acggcaccgc
      481 catgacctgg cggcgctcga cctcgtcctg gaacagcgcg ccgtcgatcg tcgtcaccct
      541 cacgcgcggg ttcagtaccg ccatcaggtt gagcgactgc acgacttccg ggcagttgtg
      601 gcaggagagc gaaatgaaga tctcgaactc gaaatcgccg tccagcgcct cgatctgctc
      661 gatgaccgcg cggtcgacct tcggcggata gccgccggcc tgcagcagcg cgagcacgag
      721 cgacgtgaac tcgtggccca tcggcaggcc ggcaaacgtg atccccatgt cgtggccgac
      781 ccgcgtgatc cggaacgaag gcgcgcgcgt gcccgccgcg acgtctgcgc cacgcagctc
      841 acggtagccg accagtggcg acaaggtcgc gatgtcctgc agcaacgcgg ccatctcgcg
      901 cgcggtctcg ctgtcgtcca gcgaggcgac gagctcgatc ggctgcgtca tgcgggccag
      961 gtagtcctgc agttgctgct tcagttcggc gtcgagcatg gtggtcttct ctcgtcaggt
     1021 tccgatcatc atcgtgggcg tcgacgaccc cctcccgccg cgaagcggga gagggcgagg
     1081 acaagccttc cgtcggggac gatccgtcca gcgtcggcag cgtcagatct tgccgaccag
     1141 ctgcagcgac ggcttgagcg tccgggaacc gtcgttccac ttggccgggc agacttcgcc
     1201 cgggttggcg gcgacgtact gcgcggcctt cagcttgcgc agcgtctcct tgacgtcgcg
     1261 cgcgatcgag ttgtcgtgga tttccagcgt cttgatcacg ccgtcggggt tcaggacgaa
     1321 cgtgccgcgc agcgccaggc cttcttcctc gatgtgcacg ccgaaggcgc gcgtcagctg
     1381 gtgcgtcggg tcgccaacca gcgggaactt cgccttgcca acggccggcg aggtctcgtg
     1441 ccacaccttg tgcgagaagt gcgtgtcggt cgtgacgata tagacctcgg ccccgagctt
     1501 ctggaactcg gtgtagttgt ccgccgcgtc ctcgacctcg gtcgggcaat tgaaggtgaa
     1561 ggcggccggc atgaagatga agaccgacca cttgccgcgc acggtctggt ccgtcacttc
     1621 gacgaacttg ccgttgtgaa acgcggtggt cttgaaaggc ttgagggtcg tgttgatcag
     1681 gttcatgcac atactccagt cgggatttcg gtgcctgcgc cggcgcggcg tctggcaatg
     1741 gcgagcattc tgcgtgcgca ggctcagtag atccaattga ttgtaacgcg aagttcgata
     1801 gtcaaggact atcggcaatc aggacgcact gcgccatgca cggattgacg cgcgaacgtc
     1861 gctcgcggtg cccgtgggac ttcgcggacg gccgcgggtt ccccgagcag gcgctcttcg
     1921 aacgacgcgg ctcgcgcgtc ttgtcggtga gcgtccgatg gccgcggcgc gccgcgccgg
     1981 ggagcatgga ggccccgagc cggagacgcc catggcccac ctcgacgcct acctgttttt
     2041 cgacggcacc tgcgccgacg cgatgcgctt ctacgagcgc gtcttccacg gccggctcga
     2101 agtactgcag acgtacgccg agtcgccggc ggcgcacgac gtcgcgccgg accgcgcgca
     2161 ccggatcatg cacgcgcgcc tcgtgatcga cgggcgcgcg ttgctcgcgt cggacgcggt
     2221 cagcgaagaa ccgttcgagg gcacccgcgg ttttgcgctc tcgatgaact acgacacggt
     2281 cgacgaagcc cagcgcgtct tcgtcgagct cggcgacggc gggacggtgc agatgccgtt
     2341 gcagcagacg ttctgggccc aggcgttcgg ca
//
LOCUS       DASPFR010000084         2379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1735155, whole genome shotgun sequence.
ACCESSION   DASPFR010000084 DASPFR010000000
VERSION     DASPFR010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735155"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VD737_02095"
     CDS             complement(<1..182)
                     /locus_tag="VD737_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HXW09381.1"
                     /translation="MPRCNFKQVDVFTAVPYLGNPVAVVLDAPGIDVATMQRYTDWTN
                     LSEATFVMPPTNPDAD"
     gene            complement(301..1488)
                     /locus_tag="VD737_02100"
     CDS             complement(301..1488)
                     /locus_tag="VD737_02100"
                     /inference="COORDINATES: protein motif:HMM:NF002326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HXW09382.1"
                     /translation="MTPRAATPGGDDEALLAPYAQRASASRGRRVPEPPPRHRSEYQR
                     DRDRIVHSTAFRRLVYKTQVFVNHEGDLYRTRLTHSLEVAQIARSVARALGLNEALAE
                     AICLAHDLGHTPFGHAGQDALNDCMREYGGFEHNLQSLRVVDELEERYAEFPGLNLTF
                     EVREGILKHCSAANARRLGDLGTRFLERRQPGLEAQLANLADEVAYNNHDVDDGIRAG
                     LVTVEELCSCRMFGRQHEAVREQYPQLAGRRLVHEVVRRMIDYAVNDLIDASAAALAA
                     AAPRDVEGVRARPQPLLTFSEPVRDEHLELKRYLREHLYKHYRVLRMTTKARRVVREL
                     FDAMLGDVDLMPTEHQETARRLEAGEGDAGRARAVADYIAGMTDRFAILEHRRLYEPG
                     ERT"
     gene            complement(1485..>2379)
                     /gene="aroB"
                     /locus_tag="VD737_02105"
     CDS             complement(1485..>2379)
                     /gene="aroB"
                     /locus_tag="VD737_02105"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HXW09383.1"
                     /translation="ELPDGEQHKTLRSVERIIDALAASRMNRDCALVALGGGVVGDIA
                     GFAAACYQRGVDYVQVPTTLLAQVDSSVGGKTGVNHPGGKNLIGAFHQPRAVLADLAT
                     LRTLPPRELRAGLAEVIKYGLIDDADFLAWLERHLEALLALDPGALAHAVRRSCEIKA
                     RIVALDEREQGPRALLNFGHTFGHAIETASGYGEWLHGEAVAIGMLLAADMSRQLGWL
                     APEDLERVRELLRRAGLPTRAPTVGARRAHELMSMDKKVLQGRIRLVLLRRLGQATVT
                     GDYPADALQATLTRHFEAAAA"
BASE COUNT          352 a          836 c          809 g          382 t
ORIGIN      
        1 tagtcggcgt cggggttggt cggcggcatc acgaacgtcg cttccgacag gttggtccag
       61 tcggtgtaac gctgcatcgt cgcgacgtcg atgccagggg cgtcgagcac gaccgcgacc
      121 ggattgccga ggtacggcac cgcggtgaag acgtcgacct gcttgaagtt gcagcgaggc
      181 acggagagaa aagtgtctag tgtccagtga tcagtgtcta gtaagaggac gctgcaccag
      241 ccgcttgggt actcagttcc tactaaccgc tagacactag acaccagaca ctgaccggcc
      301 tcacgttctt tcgcccggct cgtacaaccg acggtgctcg agaatcgcga aacggtccgt
      361 catgccggcg atgtagtcgg ccacggcccg ggcgcgcccg gcatcaccct cacctgcctc
      421 gagccggcgc gcggtctcct ggtgctcggt cggcatcagg tcgacgtccc cgagcatcgc
      481 gtcgaacagc tcgcgcacca cgcgcctggc cttcgtcgtc atgcgtagta cccgatagtg
      541 cttgtagagg tgctcgcgca ggtagcgctt gagctcgagg tgctcgtcgc gcacgggctc
      601 gctgaacgtg agcagcggct gcggtcgggc gcgcaccccc tcgacgtctc gcggcgcggc
      661 cgcggcgagg gccgcggccg aggcgtcgat gaggtcgttg accgcgtagt cgatcatgcg
      721 gcggaccacc tcgtgcacca gccggcgccc ggcgagctgc ggatactgct cgcgcaccgc
      781 ctcgtgctgg cggccgaaca tccggcagct gcagagctcc tcgaccgtga cgagaccggc
      841 acggatcccg tcgtcgacgt cgtggttgtt gtaggccacc tcatcagcca ggttcgcgag
      901 ctgtgcctcg agtcccggct ggcgacgctc caggaaccgg gtccccaggt cgcccagccg
      961 gcgcgcgttg gccgcggagc agtgcttgag gatgccttcg cgcacctcga acgtgaggtt
     1021 caggcccggg aactcggcgt agcgctcctc gagctcgtcg acgacgcgca gcgactgcag
     1081 gttgtgctcg aagccgccgt attcacgcat gcagtcgttc agcgcgtcct ggcccgcgtg
     1141 gccgaacggc gtgtgcccca ggtcgtgcgc gaggcagatc gcctcggcca gcgcctcgtt
     1201 gaggccgagg gcccgagcca cgctgcgtgc gatctgagcg acctcgagcg aatgcgtcag
     1261 ccgcgtgcgg tacaggtcgc cttcgtggtt gacgaacacc tgggtcttgt agacgagccg
     1321 ccggaacgcc gtcgaatgca cgatccggtc gcggtcgcgc tggtactcgc tgcggtgtcg
     1381 cggcggtggc tcgggtaccc gtcgcccgcg cgacgccgac gcccgctgcg cgtacggcgc
     1441 gagcagcgcc tcgtcgtcac cgcccggcgt tgccgcacgt ggcgtcatgc ggccgcggcc
     1501 tcgaagtgtc gcgtcaacgt cgcctgcaac gcgtcggccg ggtaatcgcc cgtgaccgtc
     1561 gcctggccaa ggcgacgcag cagcacgagc ctgatgcgcc cctgcagcac tttcttgtcc
     1621 atgctcatca gctcgtgcgc gcgcctcgcc ccgacggtcg gcgcccgggt cggcaacccg
     1681 gcgcgccgca gcagttcgcg cacgcgttcg aggtcctccg gcgcgagcca gccgagctgc
     1741 cgcgacatgt cggcggcgag cagcatcccg atcgcgacgg cctcgccatg cagccattcg
     1801 ccgtagccgc tcgccgtctc gatcgcgtgg ccgaacgtgt gcccgaagtt gagcagcgcg
     1861 cgcgggccct gctcgcgctc gtcgagcgcg acgatccgcg ccttgatctc gcaggaacgc
     1921 cgcacggcat gcgcaagcgc gcccggatcg agcgcgagca gcgcctcgag gtgtcgttcg
     1981 agccaggcga ggaaatccgc atcgtcgatc aggccgtatt tgatgacttc ggcgagaccc
     2041 gcgcgcagct ccctcggtgg caaggtccgc agcgtcgcga ggtccgccag cacggcgcgc
     2101 ggctggtgga acgcgcctat cagattcttg ccgcccgggt gattcacgcc ggtcttgccg
     2161 ccgaccgacg agtccacctg cgcgagcaac gtcgtcggca cctgcacgta gtcgacgccc
     2221 cgctggtagc aggcggcggc gaagcctgca atatccccga ccacaccgcc gccgagcgca
     2281 accagggcac agtcgcgatt catccggctc gcggccagtg catcgatgat ccgctcgacg
     2341 ctgcggagcg tcttgtgctg ctcgccatcg ggcaactcg
//
LOCUS       DASPFR010000085         2806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_881188, whole genome shotgun sequence.
ACCESSION   DASPFR010000085 DASPFR010000000
VERSION     DASPFR010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881188"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VD737_02110"
     CDS             complement(<1..442)
                     /locus_tag="VD737_02110"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXW09384.1"
                     /translation="MTLARLAVLFVSVGTSSAWAATAPPARAPADLYKTQCASCHEGG
                     VPRAPHSVKFQMLGPAAILSALEHGVMRTQGAALTADERRALAEFLGGATLPATRTVA
                     YKRCDPQASTFDFDRPPPLAGWSMTLEGTRFVDAERAGLTAKDVP"
     gene            667..>2806
                     /locus_tag="VD737_02115"
     CDS             667..>2806
                     /locus_tag="VD737_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXW09385.1"
                     /translation="MQNHRSASTGSRLVAAAIGLALAGAAQGQESPPAPAATGGLEEI
                     IVTGTKRFESSQDVPIAISAISETALQNAHVNDVRALQNLAPGLVLSNPAGFNATGGG
                     MRGTGTNIILVTQDAPVSFLMDEFALSHVTAQFVSLFDTQQVEVYRGPQGTLFGKNTT
                     GGVISITSKRPQLGEYGAETQVNYGQYASSGSARSLMAAVNVPMGETLAMRIAGIYDQ
                     DDGFYSDGKKTATFPGNVPLWGAFGLAPGALPPPELNTNVRGGGGHLGGKDVIAGKVK
                     FLWEPTDWYSGYLIYENVKDRSDSPPGVNESTETDLLTALGFPGIQLAGEKDVFKTLI
                     SHNQNIYMDEGHKVDTEGVYLTQSFKLGAGEVRSITGYREQQQWLPSTYTGEAFLTLF
                     DSTRNTSRRTTQQELRFVSDFDGPFNFVTGANYFHDSFDFMAVFSVGLTSLIPSIHPD
                     TGTALRPDGYVNLDLRAISDFQTQGTEQTRDEYAAYFDGNFEVTDTFKISAGVRFSKD
                     EKQFLRFVDGGGPCTALTDPQDAKTLDDGSCIDTRSQYLSRAGMTAPEVTGMHTPLPL
                     SAYGVVVDEEDDWQKTTYRVVADWKPGGDDGGMFYLSYATGFLSGGFSETCATPLRCA
                     YDPETNENVELGYKADLLDRTLRFNAALYYTKYDDLQRAVVATYFAADGTEQQETVTV
                     NTGSSKAKGVDLEVSWVPTDLWRVGASINWL"
BASE COUNT          506 a          946 c          923 g          431 t
ORIGIN      
        1 taggcacgtc ctttgcggtg agcccggccc gctccgcgtc gacgaaccgc gtgccttcga
       61 gcgtcatgct ccagccggcg agtggaggcg gacggtcgaa gtcgaaggtg ctcgcctgcg
      121 ggtcgcagcg cttgtacgcg acggtgcgcg tcgcaggcag cgtggcaccc ccgagaaatt
      181 cggccagcgc gcgccgctcg tcggcggtga gcgcggcgcc ctgcgtgcgc atgacgccgt
      241 gctcgagcgc gctgaggatc gcggcgggcc cgagcatctg gaatttcacc gagtgggggg
      301 ctcgcggtac gccgccttcg tggcaactcg cgcactgggt cttgtagaga tcggccggcg
      361 cccgggcggg cggggcggtg gcggcccacg ccgacgatgt tccgaccgat acgaacagca
      421 ccgccagcct tgcgagtgtc atcgtctgca ccgcgtcccc tcgattgctg caaagcacaa
      481 ataattcccg tctccacggc aacttagccc gaaacgatca aaaaaaacag tcaggattgc
      541 ttttaatccg cagggtcgtt atcgtccggc cgcgagtcaa aacaaacagt ccttccggtt
      601 tttattcggg gactgcaggc tgccaggcac tgcctggcct ggtcatcaca gagcaggggt
      661 ccacacatgc agaaccatcg gagcgcgtcc accggcagcc gcctggttgc agccgccatc
      721 ggacttgcgc tggcaggggc cgcgcagggt caggaatcgc cgcccgcgcc ggcggccacg
      781 ggcggactcg aggagatcat cgtcaccggt acgaagcggt tcgagtcctc gcaggacgtg
      841 ccgatcgcca tctcggcgat cagtgagacc gcgctgcaga acgcccacgt caatgacgtg
      901 cgggcgctgc agaacctggc accggggctc gtgctgtcga accccgcggg attcaacgcg
      961 accggcggcg gcatgcgcgg cacgggcacc aacatcatcc tcgtcacgca ggatgcgccg
     1021 gtcagcttcc tgatggacga gttcgcgctg agccacgtca cggcgcagtt cgtgtccctc
     1081 ttcgacacgc agcaggtcga ggtctatcgc ggcccgcagg gcacgctgtt cggcaagaac
     1141 actacgggcg gcgtgatctc gatcacttcg aagcggccgc agctcggcga gtacggcgcg
     1201 gagacgcagg tcaactacgg gcaatacgcg agcagcggct ccgcacgcag cctgatggcc
     1261 gccgtcaacg tcccgatggg cgagacgctc gccatgcgca tcgcagggat ctacgaccag
     1321 gacgacggct tctacagtga cggcaagaag accgcgacgt ttcccggcaa cgtgccgctc
     1381 tggggcgcgt tcggcctcgc gcccggcgcg ctgccgccgc ccgagctcaa cacgaacgtc
     1441 cgcggcggtg gcggccacct cggcggcaag gacgtcatcg cgggcaaggt caagttcctc
     1501 tgggagccga ccgactggta ctcgggctac ctgatctacg agaacgtcaa ggaccgttcc
     1561 gacagcccgc ccggcgtcaa cgaaagcacc gagaccgacc tgctgaccgc cctcggcttt
     1621 ccgggcattc agctggccgg ggagaaggac gtgttcaaga cgctgatctc gcacaaccag
     1681 aacatctaca tggacgaggg ccacaaggtg gacaccgaag gcgtctatct gacccagtcg
     1741 ttcaagctcg gcgcgggcga ggtccgatcg atcacgggct atcgcgagca gcagcagtgg
     1801 ctgccgagca cgtacacggg cgaggcgttc ctgacgctgt tcgactcgac gcgcaacacg
     1861 tcgcgacgca cgacccagca ggagctgcgc ttcgtgtccg acttcgacgg gccgttcaac
     1921 ttcgtgaccg gcgccaacta cttccacgat tcgttcgatt tcatggcggt gttctcggtc
     1981 ggcctcacgt cgctgattcc ctcgatccat cctgacaccg ggacggcact gcggcccgac
     2041 ggttacgtca acctggacct gcgcgcgatc agcgacttcc agacgcaggg caccgagcag
     2101 acccgagacg agtacgcggc ctacttcgac ggcaacttcg aggtcaccga tacgttcaag
     2161 atttccgcgg gcgtgcgctt cagcaaggac gagaagcagt tcctgcgctt cgtcgacggc
     2221 ggcgggccct gcaccgcgct caccgacccc caggatgcca agacgctcga cgacggcagc
     2281 tgcatcgaca cgcgctcgca gtacctgagc cgcgccggca tgaccgcgcc cgaagtcacg
     2341 ggcatgcaca cgccgctgcc gctgtcggcc tacggcgtgg tcgtggacga ggaagacgac
     2401 tggcagaaga cgacgtaccg cgtggtggcg gactggaagc cgggcggcga cgacggcggc
     2461 atgttctacc tgagctacgc gaccggcttc ctgtcgggcg gcttctcgga gacctgtgcc
     2521 acgccgctgc gctgcgcgta cgacccggag accaacgaga acgtcgagct cggctacaag
     2581 gccgacctgc tcgaccgcac gctgcgattc aacgccgccc tgtactacac gaagtacgac
     2641 gacctgcagc gtgccgtcgt cgccacctac ttcgcggccg acggcacgga gcagcaggag
     2701 acggtgacgg tcaacacggg ttcgtccaag gcgaagggcg tcgacctcga ggtgtcgtgg
     2761 gtgccgacgg acctctggcg cgtcggcgcg tcgatcaact ggctcg
//
LOCUS       DASPFR010000086        10803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_149035, whole genome shotgun sequence.
ACCESSION   DASPFR010000086 DASPFR010000000
VERSION     DASPFR010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10803
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149035"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VD737_02120"
     CDS             <1..653
                     /locus_tag="VD737_02120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXW09386.1"
                     /translation="GGRVTGVRLADRTVISTPVLISAGGPWCTRLFAKVGLASPWRLE
                     PTRIQIAHIDRPHEVIGDLPVTVDQVGGIYFRTQNRGQQIILGSIRDADEREIVPDPD
                     QFATYADDEFMRSKLYVLEHRLRGLSEVRRPRGYSGLYTINRHDFHPIVGPTPIEGLL
                     VANGMSGHGFKLAPAIGSLIARMLAGPTDSFDTRVDPSFLAFERAPIALKSLGVVA"
     gene            659..1045
                     /locus_tag="VD737_02125"
     CDS             659..1045
                     /locus_tag="VD737_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXW09387.1"
                     /translation="MPDTTFDHRPKGLAMIRRLQTGPRMSQAVVHADTVYLAGQVAAD
                     PTGDVASQTRQILDAIDALLAEAGSDKTRILSATIYLADVATFSQMNGVWDAWVPAGH
                     TPARATVEARLAAPGYKVEIQIVAAR"
     gene            complement(1231..1304)
                     /locus_tag="VD737_02130"
     tRNA            complement(1231..1304)
                     /locus_tag="VD737_02130"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1270..1272),aa:Gly,seq:ccc)
     gene            complement(1389..2144)
                     /locus_tag="VD737_02135"
     CDS             complement(1389..2144)
                     /locus_tag="VD737_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09388.1"
                     /translation="MSAPSIGVTSARAALDGETRFFVRAAAVCVVVAFAGFVSRYFAP
                     MAAGTFAARTLVHLHGLAMWMWMLLFAGQSWLAATGRLRRHRASGMAGIALFTLAVWL
                     GLSVALLQLDRQIEAGDADRARAFVALPLSLSITMVALFVAALANAKRPEAHARLMLL
                     VTLVALTPALARLVGDVSGTGAHPRNPVIAAVIVLIAIGLAATRDLHVRGRVHAAYLY
                     GGVFVAIMTLARFTLNRTDAWSGVADALRTLVH"
     gene            2350..3018
                     /locus_tag="VD737_02140"
     CDS             2350..3018
                     /locus_tag="VD737_02140"
                     /inference="COORDINATES: protein motif:HMM:NF017833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HXW09389.1"
                     /translation="MTAVQFRNPEVRLSEALQATRAAVSGHEVTLRELLGLVGEQGLL
                     IFCAILAMPFLLPVTLPFMSAALGAPMLAIGWAIVMNRLPWLPDRMLDRALPCETVQQ
                     VLERAARAASRFEHLVRPRLLRLTATAGMNVLNGVVLTLAVLLLMAPLPLVPFANTLP
                     AVAIILLCLGMAERDGALLLFGYACTLASLAYIGGLMWLAARTGSNLDAAWRGLRAMF
                     ERLF"
     gene            3081..3647
                     /locus_tag="VD737_02145"
     CDS             3081..3647
                     /locus_tag="VD737_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09390.1"
                     /translation="MRIAPGICVALACLALPIAASAAGAAQAEPASPGHPGEHHAHDD
                     ASSALVSRLELTSDGRRWPTDAALRAGMANIRAAFEADHPAIHAGRQTDTQYDALASR
                     IETEVNAIVAQCKLPPEADAQLHYVVGDLLQGVSLMRGGDPSKTRHDGAHRVHVALNA
                     YGKYFDDPAWSGTSKGADPHAQSHEHAN"
     gene            complement(3658..4590)
                     /locus_tag="VD737_02150"
     CDS             complement(3658..4590)
                     /locus_tag="VD737_02150"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXW09391.1"
                     /translation="MDRFLAIQAFAKVVESGSFARAAERLTVSTSAVSRQVSELEAHL
                     HVRLLNRTTRRLSLTEAGQSFYEHAVQLLADLDDAEASVRVAATAPKGTLRLTCGVSF
                     GIRYLAPVLADFAIEHPEVVFDLDVSDRAVDLVEEGYDLAVRIGSIGQQGMVSRRLGW
                     TQIVCCASPSYLARASAPLEVPADLARHECLSYSNVGLPNSWRFSDAAGAGDDVTARI
                     APRHRANNGQLLASLATAGLGVVYTPDFIVADEIRAGKLVRLLPNHRPPRSPIAAVYP
                     SRRHLSAKVRVIVDYLARRYAHERPWQLDGDADD"
     gene            4781..5386
                     /gene="gstA"
                     /locus_tag="VD737_02155"
     CDS             4781..5386
                     /gene="gstA"
                     /locus_tag="VD737_02155"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HXW09392.1"
                     /translation="MKLYYTPGACSQAPHIAIHELGLPYEAVKVDLATHKLDDGTDYR
                     TINPKGYVPTLELDDGTRLTEANVILQYLAEQKPGRLAPAFGSKERWTLIEWLAFIAS
                     EVHKGFGPLWNPKTPADVRERTVQALGNRFGYLAQTLGKQPFLMGNEFTIADAYLFVV
                     LNWSDLHKVDLGPWPALQQFRQRVAARPAVQATLKAEGLAK"
     gene            5457..6248
                     /locus_tag="VD737_02160"
     CDS             5457..6248
                     /locus_tag="VD737_02160"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HXW09393.1"
                     /translation="MTRLPTLFISHGSPMHAVDAGGAGQAWRALADALPRPRAILIAS
                     AHWETALPMLTGSSRLETIHDFGGFPDELYKIRYDVPGSPDLANEAATLLRAADLTPS
                     VNACRGIDHGAWVPLKWMYPDRDVPVVQLSVQPHLGTAHHLALGRALAPLTERGVLVI
                     GSGHVTHNLRDWFAGSGAATTLAYVTQFAGWLEQKLEARDLDAVVAYRDQAPGAERAH
                     PTDEHFLPLFVALGAAGEEPLAEKVYDAIEGVALSMDAYRFTQAA"
     gene            complement(6301..7602)
                     /locus_tag="VD737_02165"
     CDS             complement(6301..7602)
                     /locus_tag="VD737_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXW09394.1"
                     /translation="MSFDSLGLRPELLRAVQELGYTQATPIQSRAIPSVLEGRDLLAA
                     AQTGTGKTAAFALPLLQRLSETHPQPRGSRTPRALVLVPTRELAAQVHQSFVDFGRHL
                     QQHATLIFGGVSPRPQVETLRRGVDIVIATPGRLLDHANERNIDLRDVRHLVLDEADR
                     MLDMGFIRDIRRVLALLPPKRQNLLFSATFSEEIRGLAKGLLQDPLTVEVSPRNAPAE
                     LVEHRVHLVSQGDKRAVLSHLLRNGDSSPQTLVFTRTKHGANRLAEQLEKDGLGCAAI
                     HGNKSQNARTKALEDFKAYRVQVLVATDIAARGLDITELPQVVNYELPHVPEDYVHRI
                     GRTGRAGCAGQALSLVSDDEKDRLRAIEKVLGRKIQVLPLDRSQIQRPPGEGLAREPR
                     HVDPRAVAQAQARRPHHGTATTAARPAGSRRWQPRGARQRA"
     gene            complement(7828..9015)
                     /locus_tag="VD737_02170"
     CDS             complement(7828..9015)
                     /locus_tag="VD737_02170"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HXW09395.1"
                     /translation="MSGNRKPRESLSAAEARRLALAAQGFGQSRPPDGTLSRRDVLRT
                     VRRLGLVQIDSVNVLVRSHYLPLYSRLGAYDRAWLDELAYGGRRRRLYEYWGHEASLL
                     PVERQPLLRWRMQRARNGDGLWGSVARFGRQRRRFCSSVLAEIRDRGPLGVSEIAAAG
                     SRRGGWWGWSEGKLALEWLFWTGQVTTHARRGSFERVYDLTERALPAAVVDAPTPTTP
                     DAQRELLTIALRALGVATERDLRDYFRLGVEDTRVRIAELVEAGECVPVQVESWRGPA
                     YLASDARVPRRIDACALLSPFDSLVWYRERLQRLFAFHYRIEIYTPAHKRRHGYYVLP
                     FLLGDRLVARVDLKSDRAAGVLRVVSTHFEPTVDAAGVQPALRQELQRLAGWLGLGLN
                     PAA"
     gene            9091..10689
                     /locus_tag="VD737_02175"
     CDS             9091..10689
                     /locus_tag="VD737_02175"
                     /inference="COORDINATES: protein motif:HMM:NF009472.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HXW09396.1"
                     /translation="MIRYSADVIVIGAGIAGVTAAIELLDRGRRVLLLDRDAEENMGG
                     LAKESFGGIWFAGTPLQKRYGIKDGPEQGLRDWLSFAGFGPEDRWPRAWAEAYVGRSN
                     AEVFAWLRSVGVQFMPMPLWVERGLHGPGNSVPRWHIVWGTGHELQVVLNQHLLARPH
                     RDRLELQFDHRVEALLTTDGRVTGCRGRLEGGDQEFEAHGEAVIVASGGINGDLDRVR
                     QHWHADWSRPPETLLNGSHKFADGRMHDAAATLGARITHLDWQWNYAAGVRHWAPRKP
                     SHGLSLVPPKSALWLNWRGERIGPMPLVTGFDTHDLVAQICRQERAYSWQLLNRRIML
                     KELAISGGEFNPSFRRKNRLHVARDILLGNHWLYDQMTQLCPDVVVARTVEELVDRMN
                     ELAGDGSVDLEAVRSAATNYDRCIDLGPRFHTDEQLRRIEFARRWIGDRVRTCKFQKI
                     LDPAAGPLIAIREFIISRKSMGGLQTDLECRVLDQAGAPIPGLYAAGESAGFGGGGMN
                     GQRGLEGTFLGGCLLGGRIAGQLA"
BASE COUNT         1737 a         3737 c         3697 g         1632 t
ORIGIN      
        1 ccggaggcag agtcaccggc gtacgccttg ccgaccggac cgtcatttct acgcctgtgc
       61 tcatcagcgc tggaggacct tggtgcactc gcctgttcgc caaggtcggt ctcgcctctc
      121 cctggcgact cgagcccaca cgcatccaga ttgcgcacat cgatcgtccg catgaggtca
      181 tcggcgacct gccggtgacg gtcgatcagg ttggcggcat ctacttccgc acccagaatc
      241 gaggacagca gatcatcctc gggtccatac gggatgccga cgaacgggaa atcgttcctg
      301 accccgacca gttcgccact tatgctgacg acgagttcat gcgatcgaaa ctgtacgttc
      361 tcgagcaccg cctccgcggt ctgtccgagg tcaggcggcc acgaggctac agcggtctct
      421 atacgatcaa tcggcacgac ttccacccta tcgtcggtcc cacgcccatc gaagggctac
      481 tggtcgcgaa cgggatgagc ggtcacggct tcaaactggc acctgctatt ggctcgctga
      541 tcgcacggat gctcgccgga ccgacagact cgttcgacac gcgggtcgat ccctcattcc
      601 tcgcgtttga acgagcaccg attgcgctca agagcctcgg cgtcgtggct tagtatcatt
      661 gcccgacacc acgttcgacc accgaccgaa ggggcttgcc atgattcgtc gtttgcaaac
      721 cggtcccagg atgtctcagg ccgtcgtcca cgccgacacg gtctatctgg cgggccaggt
      781 cgctgccgac cccacggggg atgtggccag tcagacccgg cagatcctcg atgcgatcga
      841 cgcgctgctc gccgaggcag gctcggacaa gacgcgcatt ctctccgcca cgatctacct
      901 tgcggacgtc gccaccttca gccagatgaa cggcgtgtgg gatgcctggg tgcctgcagg
      961 ccatacgcca gcgcgcgcaa ctgtagaggc tcggctggcc gcgcctgggt acaaggtcga
     1021 gatccagatc gttgccgcac gctgaaccgt cctactcacc caccccgtcg cccgtcgacg
     1081 agtcagccac gcgtgatgcc atcgggaacg gacgggaacg taggcaagca gtcttgctgg
     1141 ctttattggg atgcaccgtc accatggctg gcagacgcag ctcgtgacct ctccgcacct
     1201 tcccctacgt tcttcttctt gcggaaatgg tggagcgggt gatgggaatc gaacccacgt
     1261 tagtagcttg ggaagctaca gttctaccat tgaactacac ccgcttgagc cgcggattct
     1321 aagcggcggc gtggcaggcg gcaaacgacc cggcgccgga ggatctccgc agcgtcatgc
     1381 cgcgaccgtc agtgcacgag cgtccgaagt gcatcggcca cgccggacca ggcgtccgtc
     1441 cggttcagcg taaagcgcgc cagggtcatg attgcgacga acacgccgcc gtacaggtag
     1501 gcagcgtgca cgcggccccg gacgtgcaag tcccgggtcg cggcaaggcc gatcgcgatc
     1561 aacacgatca cggcggcgat cacggggttg cgcggatgcg cgccggtgcc cgatacgtct
     1621 cccaccagac gcgcgagcgc cggcgtcagc gccaccagcg tcaccagcag catcaggcgc
     1681 gcgtgggcct cgggccgctt cgcgttggcc agcgcggcga cgaacagcgc caccatcgtg
     1741 atggagaggc tgagcggcag cgccacgaac gcacgggccc ggtccgcgtc gcccgcctcg
     1801 atctgccggt cgagctgcag cagggccacg ctcaacccca gccagaccgc cagcgtgaac
     1861 agggcgatgc cggccatgcc ggaggcacga tgccgtcgca gccgtcccgt ggccgcgagc
     1921 caggattgcc cggcgaacaa cagcatccac atccacatgg cgagcccgtg cagatgcacg
     1981 agggtgcgcg cggcgaacgt gccggccgcc atcggcgcga agtagcggga cacgaagccc
     2041 gcgaacgcca cgaccacgca gaccgccgcc gcgcggacga agaatcgcgt ttcgccgtcg
     2101 agcgccgcac gggcagacgt gacgccgatg gaaggtgcgg acacgatcaa gccccgcgtc
     2161 gtctcagcgg cgcgcagcga cgcgttcgtg ccgtcatccg gacgatcccc caggcgccca
     2221 tgggcgcgag cttgccccat tgccagccgg cgtcaactcc cgccacgtca tcggcgtggg
     2281 acaggaggtg ccggtcatgt gaaatgtgct cgcatcgctt gcccggccca ccgggtaacc
     2341 tgcgcgtgca tgacggccgt acagttccgc aatcccgagg tccgcctgtc cgaagcgctg
     2401 caggcgaccc gggccgccgt ctccgggcac gaggtcacat tgcgtgaact cctgggcctc
     2461 gtgggcgagc agggcctgct gatcttctgc gcgatcctcg ccatgccgtt cctgctgccc
     2521 gtaacgctgc ccttcatgag tgcggcgctc ggcgcaccga tgctggcgat cgggtgggcg
     2581 atcgtcatga accggctgcc ctggttgccg gaccggatgc tcgaccgcgc gttgccgtgc
     2641 gagaccgtgc agcaggtgct cgagcgcgcg gcccgtgccg cgagccggtt cgagcacctg
     2701 gtccgaccca ggctgctgcg actgaccgcg accgcgggca tgaacgtcct gaacggcgtc
     2761 gtgctcacgc tggccgtgct gctgctgatg gcgccgctgc cactggtccc cttcgccaac
     2821 acgttgccgg ccgtggcaat catcctgctg tgcctcggga tggccgagcg cgacggcgcg
     2881 cttctcctgt tcgggtacgc ctgcacgttg gcgtccctgg cctacatcgg cgggctcatg
     2941 tggctcgccg cccggacggg cagcaacctc gacgccgcct ggcgcgggct ccgcgcgatg
     3001 ttcgagcgat tgttctgagt caaggcggcc cggcggcgaa cccgtagaat cgggtccctg
     3061 tccaacaagg aggttgcctc atgcgcatcg cacccggcat ctgcgtcgcc ctcgcctgtc
     3121 ttgcgttgcc catcgctgca agcgcggccg gcgccgcgca ggcggagccc gcgtctcccg
     3181 gtcatccagg agaacaccac gcccacgacg acgcctcgtc ggcgctcgtg tcgcggctcg
     3241 aactcaccag tgacggccgg cgctggccga cggacgcggc tctgcgcgcc ggcatggcga
     3301 acatccgcgc agcgttcgag gccgaccatc ctgcgatcca cgcgggacgc cagaccgata
     3361 cgcagtacga cgcacttgcg tcgaggatcg aaaccgaggt caacgcgatc gtggcgcaat
     3421 gcaagctgcc gcccgaagcc gatgcgcagc tgcactacgt cgtgggtgac ctgctgcagg
     3481 gcgtaagcct catgcggggt ggtgatcctt cgaagacccg tcacgacggc gcacaccgcg
     3541 tgcacgtcgc gctgaatgcg tacggcaagt acttcgacga tcccgcctgg tccgggacct
     3601 cgaagggagc ggacccgcac gcgcagtcgc acgagcacgc taactgagcc ggagacctca
     3661 gtcgtccgcg tccccgtcga gttgccaggg tcgctcgtgc gcgtagcggc gcgcgaggta
     3721 gtcgacgatg acgcgaactt tcgccgacag gtgacgccgg ctcgggtaca ccgcggcgat
     3781 cggtgagcgc ggtggccggt ggttcggcag cagccggacg agctttccgg cgcggatctc
     3841 gtcggcgacg atgaagtcgg gcgtatagac gacgcccagg cccgcggtcg ccagggacgc
     3901 gagcagctga ccgttgttgg cgcgatgacg cggcgcgatg cgcgccgtga cgtcgtcgcc
     3961 cgcgcccgca gcgtcgctga agcgccagct gttgggcagg cccacgtttg aatagctcag
     4021 gcattcgtgc cgcgcgagat cggcgggcac ctcgagcggg gcgctggcgc gggccaggta
     4081 cgagggcgac gcgcagcaca cgatctgagt ccagccgagc cgacgcgaca ccatgccctg
     4141 ctgtccgatc gagccgatcc gcacggcgag gtcgtagcct tcctcgacga ggtcgaccgc
     4201 gcgatccgag acgtcgagat cgaagacgac ctcgggatgc tcgattgcga agtcggcgag
     4261 caccggagcg aggtagcgga ttccgaaact gacaccgcac gtgagccgca aagtgccctt
     4321 cggcgcggtc gcggcgacgc gcaccgatgc ttcggcgtcg tcgaggtctg cgagcaactg
     4381 caccgcgtgt tcgtagaacg attgccccgc ctcggtcagc gacagccggc gtgtcgtgcg
     4441 attgagcagc cgcacgtgca ggtgcgcctc gagttccgac acctggcgtg aaacggccga
     4501 cgtcgacacg gtcaggcgct cggcggcacg cgcgaagctg ccgctttcga cgaccttcgc
     4561 aaaggcctgg atggcgagaa atcgatccac gcgacgacct cgacgtgacc cggccggcgc
     4621 gaaccgcgcc cagcgtcgcc gcggcgggga ccccggaagg cgacaagtta ttgtcgcata
     4681 ttccgcaaga atgaatcctg tggttatggc tttatctcgc catccagaat gccgaccatg
     4741 cgcgcacggc cactcccggc cccccgcaga ggacaaacgc atgaagctgt actacacgcc
     4801 cggcgcctgc tcacaggccc cgcacatcgc catccacgaa ctcggcctgc cgtacgaggc
     4861 cgtcaaggtg gacctcgcga cgcacaagct cgacgacggc accgactacc ggacgatcaa
     4921 cccgaagggt tatgtgccga cactcgagct cgacgacggc acgcgcctca cggaagcgaa
     4981 cgtgatcctg cagtacctcg ccgagcagaa gcccggccgt ctcgcgcccg cgttcgggtc
     5041 gaaggagcga tggacgctca tcgagtggct cgcgttcatc gcgagcgagg tgcacaaggg
     5101 cttcgggccg ctgtggaacc ccaagacacc cgccgacgtg cgtgaacgca ccgtgcaggc
     5161 gctcggcaac cggttcggct acctcgcgca gacgctcggc aagcagccgt tcctgatggg
     5221 caacgagttc acgatcgcgg acgcctacct gttcgtggtg ctgaactggt ccgacctgca
     5281 caaggtggac ctgggcccgt ggccggcgtt gcagcagttc cggcaacgcg tcgccgcccg
     5341 cccggcggtg caagccaccc tcaaggccga gggcctggcg aagtaggaag cagcgtcgac
     5401 cgccgcgagg gctgccgtag ggcagccctc agcggcgtca cggaggaggt ttacgcatga
     5461 cccgtctgcc caccctgttc atctcgcacg gctcgccgat gcacgccgtc gacgccggcg
     5521 gcgccggcca ggcgtggcgg gcgttggccg atgcgctgcc ccggccgcgg gcgatcctga
     5581 tcgcgtctgc gcactgggag accgcgctgc ccatgctgac gggctcttcg aggctcgaga
     5641 ccattcacga tttcggcggc tttccggacg agctgtacaa gatccgctac gacgtgccgg
     5701 gctcccccga cctcgcgaac gaagcggcca cgctgctgcg cgcggccgac ctgacgcctt
     5761 cggtgaacgc atgccgcggc atcgaccacg gcgcgtgggt gccgctgaag tggatgtacc
     5821 cggaccgcga tgtgccggtc gtgcagctct cggtgcagcc gcacctcggc acggcacatc
     5881 acctcgcgct cgggcgcgcg ctcgcgccgc tgaccgagcg tggcgtgctg gtgatcggtt
     5941 ccggccacgt cacgcacaac ctgcgcgact ggttcgcggg aagcggcgcg gcgacgacgt
     6001 tggcctacgt gacgcagttc gcgggctggc tcgaacagaa gctcgaggcg cgcgacctcg
     6061 acgccgtggt ggcctatcgc gaccaggcgc cgggcgcgga acgtgcgcac cccaccgacg
     6121 agcacttcct gccgctgttc gtggcgctcg gggccgcggg cgaggagccc ctcgccgaga
     6181 aagtctacga cgcgatcgaa ggcgtcgcgc tgtcgatgga cgcctatcgg ttcacgcagg
     6241 cggcgtgaca ccagtgatca gggacgctcg gtcatccagg ccgagcgtcc cgcgtgctga
     6301 tcaggccctc tgacgagcgc cgcgaggttg ccagcggcga cttccggcgg gacgcgccgc
     6361 cgtcgtcgcc gtcccgtgat gggggcgtct ggcctgcgcc tgtgccacag cccgggggtc
     6421 gacgtgccgc ggttcgcgtg ccaggccttc gcccggcggt cgctgaatct ggcttcggtc
     6481 gagcggcagc acctgaatct tccggcccag cactttctcg atcgcgcgca ggcgatcctt
     6541 ctcgtcgtcg gagaccagcg acagcgcctg ccctgcgcac cctgcgcggc ccgtgcgacc
     6601 gattcggtgc acgtagtcct cgggcacgtg cggcagctcg tagttgacca cctgcggcag
     6661 ttcagtgatg tcgaggccgc gcgccgcgat gtcggtcgcg accagcacct gcaccctgta
     6721 ggccttgaag tcctcgagcg ccttcgtgcg cgcgttctgc gacttgttgc cgtggatcgc
     6781 ggcgcaaccc agaccgtcct tctcgagctg ctcggcgagc cggttggcgc cgtgcttggt
     6841 gcgagtgaac acgagcgtct gcggcgagga gtcgccgttg cgcagcaggt ggctcagcac
     6901 ggcgcgcttg tcgccctgcg agacgaggtg cacccgatgc tcgaccagct ccgccggcgc
     6961 gttgcgcggc gacacttcca cggtcagcgg atcctgcagc aggcccttgg ccagcccgcg
     7021 gatctcctct gagaaggtcg ccgagaacag caggttctgg cgcttcggcg gcagcagcgc
     7081 gagcacgcgc cggatgtcgc ggatgaaacc catgtcgagc atgcggtctg cctcgtcgag
     7141 gacgaggtgc cgcacgtcgc gcaggtcgat gttgcgctcg ttcgcgtggt cgagcaggcg
     7201 gcccggcgtc gcgatcacga tgtcgacgcc gcggcgcagc gtttcgacct gcggccgggg
     7261 gctcacgccg ccgaagatca gcgtcgcgtg ctgctgcagg tgacggccga aatccacgaa
     7321 cgactggtgg acctgcgccg cgagctcgcg cgtcggcacc agcacgagcg cacgcggagt
     7381 gcgcgagcct cgcggttggg ggtgcgtctc gctcaggcgc tgcagcagcg gcagtgcaaa
     7441 tgcggccgtc ttgccggtgc cggtctgcgc ggcggcgagc aggtcgcggc cttcgagcac
     7501 ggacgggatc gcacgggatt ggatgggagt ggcctgcgta tagccgagtt cttgaacggc
     7561 gcgcagcagc tcgggccgga ggcccaggga atcaaaagac atgagtgtgg tgtaccaggc
     7621 acccgaccgg acggggcgcc gttgaggagg aatcgagacg cgaaatgcag acgtcgatca
     7681 gtctgcagga ggggccgcaa gggctggaaa ggaagcgtgt acgctctttc cgcctgaaga
     7741 tcctcgtgcg caggccgggc tgcgcggcgg caaactgtgc acgcagtatg cctgaactgc
     7801 gtcgccggcg cgaatcggcg ccacgaatca cgccgccggg ttgagcccaa ggccgagcca
     7861 gcccgccagc cgctgcagtt cctgtcgcag cgcgggttgc acgccggcgg cgtccacggt
     7921 cggctcgaag tgcgtgctga ccacacgcaa caccccggcc gcgcgatcgg atttcaggtc
     7981 gacccgtgcg accagtcggt caccgagcag gaacggcagc acgtaataac cgtgccggcg
     8041 cttgtgcgcc ggcgtgtaga tctcgatgcg gtaatgaaac gcgaacagcc gctgcagccg
     8101 ctcgcgatac cagaccagcg agtcgaatgg ggacagcagc gcgcaggcgt cgatccggcg
     8161 gggcacgcgg gcgtcgctcg ccaggtatgc cgggccccgc cagctttcga cctgcaccgg
     8221 cacgcactcg cccgcttcga cgagctcggc gatgcgcacg cgcgtgtctt cgacgcccag
     8281 tcgaaaatag tcgcgcaggt cgcgctcggt tgccacgccc agcgcgcgca gcgcgatcgt
     8341 gagcagctcg cgctgcgcgt ccggcgtcgt cggcgtgggg gcgtcgacca cggccgccgg
     8401 caacgcgcgc tcggtcaggt cgtagacgcg ctcgaagctg ccgcggcgtg cgtgcgtcgt
     8461 gacctggccc gtccagaaga gccactcgag cgcgagcttg ccctcgctcc atccccacca
     8521 gccgccgcgc cggcttcccg ccgcggcgat ctccgaaacc ccgagcggtc cgcgatcgcg
     8581 gatttcggcg aggaccgacg agcagaagcg tcgacgctgc cggccgaagc gggcgacgct
     8641 gccccagagt ccgtcgccgt tcctggcgcg ctgcatgcgc cagcgcagca gcggctggcg
     8701 ctccaccggc aggagcgagg cttcgtggcc ccagtattcg tacagccgcc ggcgccggcc
     8761 gccgtaggcg agctcgtcga gccaggccct gtcgtaggcg ccgagccgcg agtacagcgg
     8821 caggtagtgt gaccgcacga gcacgttgac ggagtcgatc tgcacgagcc cgagccgacg
     8881 gaccgtgcgc aggacgtcgc ggcgcgaaag cgtgccatcc gggggcctcg actggccgaa
     8941 gccctgcgcg gcgagcgcca ggcgccgcgc ctcggcggcg ctcagagatt cgcgcggctt
     9001 tcggttcccg ctcatgcggc ggatcataac ggcgcgatac gcttatcggt cggacgccgc
     9061 cgtcgcaccg gtcggcgcgt ggagggcccg atgattcgct actcggcgga cgtgatcgtg
     9121 atcggggccg gcatcgccgg cgtcaccgca gccatcgaac tgctcgatcg cggtcgccgc
     9181 gtgcttctgc tcgaccgcga cgccgaagaa aacatgggcg gtctcgccaa ggagagcttc
     9241 ggcggcatct ggttcgccgg cacgccgctg cagaaacgct acggcatcaa ggacgggccc
     9301 gagcagggac tgcgcgactg gctgtcgttc gcgggcttcg gccccgagga ccgctggccg
     9361 cgggcctggg ccgaagccta cgtcgggcgc tcgaacgcgg aggtgttcgc gtggctgcgc
     9421 agcgtcggcg tgcagttcat gccgatgccg ctctgggtcg agcgcggcct gcacgggccg
     9481 ggcaattcgg tgccgcgctg gcacatcgtc tggggcaccg gacacgagct gcaggtggtg
     9541 ctgaaccagc acctgctcgc gcgtccgcac cgcgaccggc tggagttgca gttcgaccat
     9601 cgcgtcgagg cgctgttgac gaccgatggc cgcgtgaccg gctgccgggg tcgcctcgag
     9661 ggcggcgacc aggaattcga ggcgcacggc gaggccgtca tcgtcgcgag cggcggcatc
     9721 aacggcgacc tcgaccgcgt ccggcaacac tggcacgccg actggtcgcg cccgccggag
     9781 acgctgctca acggatcgca caagttcgcc gacgggcgga tgcacgacgc cgccgcgacg
     9841 ctcggcgcgc gcatcacgca cctcgactgg cagtggaact acgccgcggg cgtgcgtcac
     9901 tgggcgccgc gcaagccctc gcacgggctg tcgctggtgc cgcccaagtc cgcgctgtgg
     9961 ctgaactggc gcggtgaacg catcggcccg atgccactcg tcacgggctt cgacacgcac
    10021 gacctcgtcg cgcagatctg ccggcaggag cgcgcgtatt cctggcagct gctcaatcga
    10081 cgcatcatgc tcaaggagct cgccatctcg ggcggcgagt tcaacccgtc gttccggcgc
    10141 aagaaccgtc tgcacgtcgc gcgcgacatc ctgctcggga accactggct ctacgaccag
    10201 atgacgcagc tctgccccga cgtcgtggtg gcgaggaccg tcgaggagct ggtcgacagg
    10261 atgaacgagc tggccggcga cgggtcggtc gacctcgagg cggtgcgcag tgccgcgacg
    10321 aactacgacc gctgcatcga cctggggccc cggttccaca ccgacgagca gctgcgtcgc
    10381 atcgagttcg cgcgtcgctg gatcggcgat cgcgtgcgca cgtgcaagtt ccagaagatc
    10441 ctcgacccgg ccgcgggacc gctgatcgcg atccgcgaat tcatcatcag ccgcaagagc
    10501 atgggcggac tgcagacgga cctcgagtgc cgcgtgctcg accaggcggg agcgccgatt
    10561 cccgggctct atgccgcggg cgagagcgcc ggcttcggcg gcggcggcat gaacggccag
    10621 cgcgggctgg aaggcacgtt cctgggcgga tgcctgctcg gcgggcgcat cgccggccag
    10681 ctcgcctgaa tatggggaca ctccggaatt ccctgcaatt ggggacactc ctcaattcgg
    10741 caatcgccgc ggaaaaatag acctccgtga accgggcccg ctgcccgcag gaaatcattc
    10801 ctc
//
LOCUS       DASPFR010000087         4182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_149896, whole genome shotgun sequence.
ACCESSION   DASPFR010000087 DASPFR010000000
VERSION     DASPFR010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149896"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="VD737_02180"
     CDS             complement(<1..766)
                     /locus_tag="VD737_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HXW09397.1"
                     /translation="MADWTDRMNLVCFEPLKYERPWELETYLKIGGYEAWKRILAEKP
                     PREQVIEQVKASGLRGRGGAGFPTGLKWSFMPRNSPVQKYMVCNSDESEPGTCHDRDI
                     LRYNPHSLIEGMAIGGYATNSTVGYNYIRGEFMAEPIPRFEAALKEAYAAGLLGKDIQ
                     GSGVDFDLYTFVGAGAYICGEETALLESLEGKQGRPRFKPPVPANFGLYGKPTTINNA
                     QSYASVPTILRNGPDWFAGLGPPNSGGTVIFSVSGHV"
     gene            complement(801..1307)
                     /locus_tag="VD737_02185"
     CDS             complement(801..1307)
                     /locus_tag="VD737_02185"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HXW09398.1"
                     /translation="MSETQSHDAGEALSPHVREEIDHWLTKFPADRPRSAVLAALHAV
                     MHEKGYLPTESMDAVAAYLGIPAIQVYEAATFYSMFETKPTGRHHISVCTNISCMLCG
                     GESILEHIQRRLGIRLGESTPDGRFYLKQEEECLAACNGAPMMMVNHVYFEHLTPEKV
                     DRILDDHK"
     gene            complement(1304..2557)
                     /locus_tag="VD737_02190"
     CDS             complement(1304..2557)
                     /locus_tag="VD737_02190"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HXW09399.1"
                     /translation="MAEIRSYTMNFGPQHPAAHGVLRLVLEMDGEVVQKADPHVGLLH
                     RATEKLAESKPYNQSIGYMDRLDYVSMMCNEHAYVRAIEKLLGIEAPIRAQYIRTMFD
                     EITRILNHLMWLGAHGLDIGAMTMFLYCFREREDLMDCYEAVSGTRMHATYYRPGGVY
                     RDLPDTMPRYAPSRFRSRKEIDRLNEARSGSLLDFIDDFTARFPKAVDEYETLLTDNR
                     IWKQRTVNIGVVSAERAMNLGFTGPVLRGSGVAWDLRKKQPYAVYDRMDFDIPVGVNG
                     DCYDRYLVRVEELRQSNRIVKQCVDWLRANPGPVMVDDHKVTPPSREQMKGDMESLIH
                     HFKLFTEGYCVPEGEVYSAVEAPKGEFGIYLVSDGANKPYRLKCRAPGFPHLAALEEM
                     TTGHMLADVVAVIGTLDIVFGEIDR"
     gene            complement(2550..3311)
                     /locus_tag="VD737_02195"
     CDS             complement(2550..3311)
                     /locus_tag="VD737_02195"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HXW09400.1"
                     /translation="MNQALEALAEQISARFGDKVARIASRCDELTFEVAPEGLVELCT
                     ALRDEPAWSFEQLTDLCGVDYLHHGRTEWNTDTATFRGFSRGVDRHSARALAVERADV
                     RPARLPARDFDPEPPHAEERRFAVVYHLLSLRNNRRLRLRAWCPAGEPPLIDSVVTVW
                     KGADWFEREAFDLYGILFRGHPDLRRLLTDYGFLGHPFRKDFPLTGNVEVRYDPTKGR
                     VVYEPVTIEPRTLVPRVIREDNRYAPALKDGPRNG"
     gene            complement(3333..3788)
                     /locus_tag="VD737_02200"
                     /pseudo
     CDS             complement(3333..3788)
                     /locus_tag="VD737_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005914280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            complement(3815..4171)
                     /gene="ndhC"
                     /locus_tag="VD737_02205"
     CDS             complement(3815..4171)
                     /gene="ndhC"
                     /locus_tag="VD737_02205"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HXW09401.1"
                     /translation="MLAEYLPILIFLAVAGALGVLLLVLGYLFAPRKPDPEKLSPYEC
                     GFEAFENTRMKFDVRYYLVAILFIVFDLEIAFLFPWAVALDAVGTVALAAMGVFLLVL
                     VVGFIYEWKKGALEWD"
BASE COUNT          671 a         1383 c         1415 g          713 t
ORIGIN      
        1 cgacgtggcc cgagaccgag aagatcacgg tgccgccgga gttcggtggc ccgaggccgg
       61 cgaaccagtc gggcccgttg cgcaggatcg tcggcaccga cgcatagctc tgcgcgttgt
      121 tgatcgtggt cggcttgcca tacagcccga agttcgcggg aaccggtggc ttgaaccgcg
      181 ggcggccctg cttgccctcg agcgattcga gcagcgccgt ctcctcgccg cagatgtatg
      241 cgcctgcgcc gacgaaggtg tagaggtcga agtcgacgcc ggagccctgg atgtccttgc
      301 cgagcaggcc cgccgcgtag gcctctttca gcgcggcttc gaagcgcggg atcggctccg
      361 ccatgaactc gccgcggatg tagttgtagc caaccgtcga attggtcgcg tagccgccga
      421 tcgccatgcc ttcgatcagc gaatgcgggt tgtatcgcag gatgtcgcgg tcgtggcagg
      481 tgccgggctc cgactcgtcg gagttgcaga ccatgtactt ctgcaccggc gagttgcgcg
      541 gcatgaacga ccacttgagg ccggtcggga aacccgcgcc gccacggccg cgcaggcccg
      601 aggccttcac ctgttcgatg acctgctcgc gcggcggttt ctcggccagg atcctcttcc
      661 acgcctcgta gccgccgatc ttcaggtacg tctcgagctc ccacgggcgc tcgtacttga
      721 gcggctcgaa gcagacgagg ttcatgcgat cagtccagtc ggccacgcga atcagctcct
      781 gcgcaaggac gggtggtgca tcacttgtgg tcgtcgagga tcctgtcgac cttctcgggc
      841 gtcaggtgct cgaagtacac gtggttcacc atcatcatcg gcgcgccgtt gcaggcggcg
      901 agacactcct cttcctgctt gaggtagaac cgcccgtccg gcgtgctctc gccaagcctg
      961 atcccgagcc ggcgctggat gtgctcgagg atcgactccc cgccgcagag catgcacgag
     1021 atgttcgtgc acaccgagat gtggtgacgg cccgtgggct tcgtctcgaa catcgagtag
     1081 aacgtcgcgg cttcgtagac ctggatcgcc gggatgccaa ggtacgccgc caccgcgtcc
     1141 atcgactcgg tgggcaggta gcccttctcg tgcatcacgg cgtgcagcgc cgccagcacg
     1201 gccgaccgcg ggcgatccgc cggaaacttg gtgagccagt ggtcgatctc ctcgcgcacg
     1261 tgcggggaca gcgcctcgcc ggcgtcgtgg gactgcgtct cgctcatcgg tcgatctctc
     1321 cgaacacgat gtccagggtg ccgatcaccg ccaccacgtc ggccagcatg tgacccgtgg
     1381 tcatttcctc gagcgccgcg agatgcggga agcccggggc cctgcacttg agccggtacg
     1441 gcttgttggc gccgtccgag acgaggtaga tgccgaactc gcccttcggc gcctcgacgg
     1501 cggaatagac ttcgccctcc ggcacgcaat agccctccgt gaacagcttg aagtggtgga
     1561 tcagcgattc catgtcgccc ttcatttgct cgcggctcgg cggcgtgacc ttgtggtcgt
     1621 ccaccatcac cgggcccggg ttggcgcgca gccagtcgac gcactgcttg acgatccggt
     1681 tggactgtcg cagctcctcg acgcgcacga ggtagcggtc gtagcagtcg ccgttgacgc
     1741 ccaccgggat gtcgaagtcc atgcggtcat agaccgcgta cggctgcttc ttgcgcaggt
     1801 cccaggcgac gcccgagccg cgcagcaccg ggcccgtgaa gccgaggttc atcgcgcgct
     1861 cggccgacac cacgccgatg ttgacggtgc gctgcttcca gatgcggttg tcggtgagca
     1921 gtgtctcgta ctcgtcgacc gccttgggga accgcgctgt gaagtcgtcg atgaagtcga
     1981 gcagcgatcc cgagcgcgcc tcgttgagcc ggtcgatctc cttgcgactg cgaaagcgcg
     2041 acggcgcata gcgcggcatc gtgtcgggga ggtcgcggta gacgccgccc ggccggtagt
     2101 acgtggcgtg catgcgcgtg ccggacaccg cctcgtagca gtccatcagg tcttcgcgct
     2161 cgcggaagca gtacaggaac atcgtcatgg cgccgatgtc gaggccgtgc gcaccgagcc
     2221 acatcaggtg gttgaggatg cgcgtgatct cgtcgaacat cgtgcggatg tactgagcgc
     2281 ggatcggcgc ctcgatgccg agcaacttct cgatcgcgcg cacgtacgcg tgctcgttgc
     2341 acatcatcga gacgtagtcg agccggtcca tgtagccgat cgactggttg tacggcttgg
     2401 actccgcgag cttctccgtc gcacggtgca gcaggccgac gtgcggatcg gccttctgca
     2461 cgacctcccc gtccatctcg agcaccaggc gcagcacgcc gtgcgcggcc gggtgctgcg
     2521 gcccgaagtt catggtgtag ctgcggatct cagccattgc gcggaccgtc cttgagcgcc
     2581 ggcgcgtagc ggttgtcctc gcggatcacc cgcggcacga gcgtgcgcgg ctcgatggtg
     2641 acgggctcgt agacgacgcg gcccttcgtc gggtcgtagc ggacctcgac gttgccggtg
     2701 agcgggaagt ccttgcggaa cggatggccg aggaagccgt agtcggtcag caggcgccgc
     2761 aggtccggat ggccccgaaa caggatgccg tacaggtcga aggcttcacg ctcgaaccag
     2821 tcggcgccct tccagaccgt cacgaccgaa tcgatcagcg gtggctcgcc cgccggacac
     2881 cacgcccgca gccgcagccg acggttgttg cgcagcgaca gcagatgata gaccactgcg
     2941 aaacgccgct cctcggcatg cggcggctcg gggtcgaagt ctcgggccgg cagccgggcg
     3001 gggcgtacgt ccgcgcgctc cactgcgagc gccctcgccg agtgacgatc gacgccgcgg
     3061 ctgaagccac ggaacgtcgc ggtgtccgtg ttccactccg tgcggccgtg gtgcaggtag
     3121 tcgacgccgc agaggtcggt cagctgctcg aagctccagg cgggctcgtc gcgcagcgcg
     3181 gtgcagagct cgacgagccc ctcgggcgcg acctcgaacg tgagctcgtc gcagcgcgag
     3241 gcgatgcgcg ccaccttgtc gccgaaccgg gccgagatct gctcggcgag tgcttccagc
     3301 gcctggttca tggttcctgc cagaggtcgt gctcagcgcg caatcgtctg cgtgcgttgg
     3361 atcttcttct gcagctggat cagcccgtac agcagcgctt ccgcatgcgg cggacagccc
     3421 ggcacgtaga cgtcgaccgg cacgatgcgg tcacagccgc gcacgaccgc gtacgagtag
     3481 tggtagtagc cgccgccgtt cgcgcaggag cccatcgaga tcacccagcg cggctcgggc
     3541 atctggtcgt agaccttgcg cagcgcgggg gccatcttgt tgacgagggt gccggccacg
     3601 atcatgacgt ccgactgccg ggggctcggc cggaacacga tgccgaaccg gtcgaggtcg
     3661 tagcgcgcgg cgccggcgtg catcatctcg accgcgcagc aggcgagccc gaacgtcatc
     3721 ggccacatcg aacccgtgcg cgcccagttc atgagcgcgt ccaccgtggt cgtgaagacg
     3781 ccgcgctccg gcgcgtcgtc gcggctcggt gcctctagtc ccattcgagc gcccctttct
     3841 tccactcgta gatgaagccg accaccagca cgagcaggaa cacgcccatg gccgcgagcg
     3901 cgacggtgcc caccgcatcg agcgcgacgg cccacgggaa caggaaggcg atctcgagat
     3961 cgaacacgat gaacaggatc gcgacgaggt agtagcgcac gtcgaatttc atgcgcgtgt
     4021 tctcgaaggc ctcgaagcca cactcgtacg gcgagagctt ctcggggtct ggtttgcgcg
     4081 gcgcgaacag gtagccgagt acgagcagca gcacgccgag cgcgccggcg actgctagaa
     4141 agatcaggat cggcaggtat tccgcgagca tcgcgtggcg gg
//
LOCUS       DASPFR010000088         3586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1736578, whole genome shotgun sequence.
ACCESSION   DASPFR010000088 DASPFR010000000
VERSION     DASPFR010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736578"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VD737_02210"
     CDS             complement(<1..365)
                     /locus_tag="VD737_02210"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HXW09402.1"
                     /translation="MAKPAINNMAVFCDFENVALGVRDARYSKFDIDKVLERLLLKGS
                     IVVKKAYCDWERYKEFKAPMHEASFELIEIPHVRMSGKNSADIRLVVDALDLCYTKEH
                     VDTFVIVSGDSDFSPLVSKL"
     gene            complement(375..998)
                     /locus_tag="VD737_02215"
     CDS             complement(375..998)
                     /locus_tag="VD737_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HXW09403.1"
                     /translation="MTAGAYVALAATFAVFALTPGPAVAAIVARAVADGAKPALALNA
                     GVLTGDLLFLGLAAAGMAAAARSMSELFTVLRWLGVAYLTWQGVVLWRTRPRVVVPAG
                     GGARHEAHYWRNYGAGLLLMFGHVQAILFYAALLPGFVDMATLTAVDLGLIALLLLAI
                     VGGANATYALLAARARGFFADERAQRALHQVAGSMMFVAAVLVATRV"
     gene            complement(1008..1982)
                     /locus_tag="VD737_02220"
     CDS             complement(1008..1982)
                     /locus_tag="VD737_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HXW09404.1"
                     /translation="MLRLLIPTGSGYTWVEHAPPAPFPDEFLWADLFEPTSDEERAVE
                     ALLTVDVPTRDEMKEIETSNRLYEEGSAVYMTATVGARLDTERPESSAITFILANGRM
                     ITNRYVDPTPVRRFMQYVERSPTACTSAPTLFAGLLEAFVERIADLLERVQLELDTVS
                     MSIFPRNGENSPTSAELHAHIKQIGADGDLVSKARESLVSFQRILMYVQQSQVVSLSQ
                     DQRGRFKSTLRDVQSLSDHASFLNTKIQFLLDAVLGLINLEQNNIIKIFSVAAVMFLP
                     PTLVASIYGMNFHFMPELDEHWGYPFALLAMAVSAVVPYIYFRRKGWL"
     gene            complement(2067..2702)
                     /locus_tag="VD737_02225"
     CDS             complement(2067..2702)
                     /locus_tag="VD737_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09405.1"
                     /translation="MHTKPVFAARLLAIGMLLAVLPRGAGAEEPPYALLNEWWQWALS
                     IPSAVNPILDNSGKSCSIGQRGNLWLLAGNTGGRTLRDCTVPAGSRALIPVHNTFCLP
                     DGQFTEQQCLDAVVADWNSFTLFEATLDGVPQVLIDQPAVPGESAFTFTVPRNGIFGF
                     RPGLYRASIAAGRWAIVDLGEPGIRTLRVRALNTSGFMVDVTYRLSVAEVH"
     gene            complement(2898..3548)
                     /locus_tag="VD737_02230"
     CDS             complement(2898..3548)
                     /locus_tag="VD737_02230"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HXW09406.1"
                     /translation="MNTRTVAGRVRQAFLAGAMLLAAGSASAAVQLQQGVNYTLLQPA
                     QPTSVAPGKVEVVEVFWYACGHCYLLEPRLEAWNRNGRPANAELVRLPATWNNVLKTH
                     ARVFYTMELLGKHELNPEIWREINVKGNRLDTPEKIEAFFTSRGVSKADFQRAFASFA
                     MDSKVAKAEDLNRRYKITGTPTIVVNGKYVTDVSMAGSEEKLFEVVNALVAKESTR"
BASE COUNT          579 a         1175 c         1258 g          574 t
ORIGIN      
        1 agcttgctga cgagcggcga gaagtccgag tcgccgctca cgatcacgaa cgtgtcgacg
       61 tgctccttgg tgtagcagag gtccagcgcg tcgacgacga gccggatgtc ggccgagttc
      121 ttgcccgaca tccgcacgtg cgggatctcg atcagctcga acgacgcctc gtgcatcggc
      181 gccttgaatt ccttgtagcg ctcccagtcg cagtacgcct tcttgacgac gatgctgccc
      241 ttgagcagca gccgttcgag gaccttgtcg atgtcgaact tcgagtaacg ggcgtcgcgg
      301 acgccgagcg ccacgttctc gaagtcgcag aacaccgcca tgttgttgat cgcgggcttg
      361 gccatgccgc cagtttatac cctcgtcgcg accagcacgg cggctacgaa catcatcgac
      421 cccgccacct ggtgcagtgc gcgctgggcg cgctcgtccg cgaagaagcc gcgcgcacgc
      481 gccgcgagca gcgcgtacgt ggcgttcgcc ccgccgacga tcgcgagcag cagcagtgcg
      541 atcagtccga ggtcgaccgc cgtcagcgtc gccatgtcga cgaagccggg cagcagcgcc
      601 gcgtagaaca ggatggcctg cacgtgaccg aacatcagca gcagcccggc accgtagttg
      661 cgccagtagt gcgcttcgtg acgggcgccg cccccggcgg gcaccaccac ccgcggtcgc
      721 gtgcgccaca gcacgacgcc ctgccaggtg aggtaggcga ccccgagcca gcgcagcacc
      781 gtgaacaact cgctcatcga ccgggcggcc gcggccatgc cggcggcggc caggccgagg
      841 aacagcaggt cgccggtcag cacgcccgcg ttgagcgcga gcgccggctt cgcgccgtct
      901 gcgacggcac gtgccacgat cgccgcgacc gccgggcccg gcgtgagggc gaacaccgcg
      961 aacgtggccg cgagcgcgac gtaggcaccc gcggtcatgg cggtccgcta cagccagccc
     1021 ttgcggcgga agtagatgta cggcaccacg gccgacacgg ccatggccag cagcgcgaag
     1081 gggtagcccc agtgctcgtc gagctccggc atgaagtgaa agttcatgcc gtagatgctc
     1141 gcgacgagcg tcggcggcag gaacatcacc gcggcgaccg agaagatctt gatgatgttg
     1201 ttctgctcga ggttgatcag cccgagcacg gcgtccagca ggaactggat cttggtgttc
     1261 aggaacgaag cgtggtcgga cagcgactgc acgtcgcgca gcgtggactt gaagcgaccg
     1321 cgctggtcct gcgagagcga gaccacctgg ctctgctgca cgtacatcag gatgcgctgg
     1381 aagctgacga ggctttcccg cgccttcgag acgaggtcgc cgtcggcgcc gatctgcttg
     1441 atatgcgcgt gcagctcggc gctcgtgggc gagttctcgc cgttgcgcgg gaagatcgac
     1501 atcgagacgg tgtcgagctc gagttgcacg cgctcgagca ggtcggcgat ccgctcgacg
     1561 aacgcctcga gcaggcccgc gaacagcgtc ggcgccgacg tgcaggcggt cggtgatcgc
     1621 tcgacgtact gcatgaaccg gcgcacgggc gtcggatcga cgtagcggtt cgtgatcatg
     1681 cggccgttcg cgaggatgaa cgtgatcgcg ctgctctcgg gccgctccgt gtcgagccgg
     1741 gcgccgaccg tggccgtcat gtagacggcg ctgccttcct cgtacagccg gttcgaggtc
     1801 tcgatctcct tcatctcgtc ccgcgtgggg acgtcgaccg tcagcagcgc ctcgacggca
     1861 cgctcctcgt cgctggtcgg ctcgaacagg tctgcccaga ggaactcgtc cgggaacggc
     1921 gcgggaggcg cgtgctcgac ccaggtgtag ccgctgccgg tggggatcag gagtcgaagc
     1981 atgcgaacgg accgcgctgc gaggtgcggg gctttgtagc acagggacac gccgggcggg
     2041 caggggcgcc ggcccggcgc gcgtgctcag tgcacttcgg cgacgctcag acggtaggtc
     2101 acgtcgacca tgaagccgga ggtgttgagc gcgcggacac gcagcgtgcg gatgcccggc
     2161 tcgcccaggt cgacgatggc ccagcgaccg gccgcgatgg acgcgcggta gaggccgggg
     2221 cggaagccga agatcccgtt gcgaggcacc gtgaacgtga atgccgactc accgggcacc
     2281 gcgggctggt cgatcagcac ctgtggcacc ccgtcgaggg tcgcctcgaa gagcgtgaac
     2341 gaattccagt cggcgacgac ggcgtccagg cactgctgct ccgtgaactg gccgtccggc
     2401 aggcagaacg tgttgtgtac cgggatcaac gcgcgcgagc cggccggcac ggtgcaatcg
     2461 cgcagcgtgc gaccgccggt gttgccggcg agcagccaga ggttgccacg ctggccgatc
     2521 gagcagctct tgccggaatt gtcgaggatc gggttgaccg cggacggtat ggacagggcc
     2581 cactgccacc actcgttgag cagcgcgtag ggcggctcct ccgcgccggc gccgcgcgga
     2641 aggacggcca gcagcatgcc gatggccagt agccgggcgg cgaacacggg cttcgtgtgc
     2701 atctcgaggc tcccttggcg aggtgatctt cgtcaccgtc ggcgacgtcc gatgcggcgg
     2761 acgccccggc gctaacgtcc cacgagggcg ctcttatgta aagtcccagg gagtctccgc
     2821 agtggactcg gcggtcgaca aaaaagccgc ctcggcgaac cgggcggctt cgagcgtgtc
     2881 gactgcgcgc actcgcctca gcgcgtcgac tccttggcga cgagagcatt gaccacttcg
     2941 aagagctttt cttcgctgcc cgccatgctc acgtccgtga cgtacttgcc gttgacgacg
     3001 atggtgggcg tgccagtgat cttgtagcgg cggttgaggt cttcggcctt cgcgaccttg
     3061 ctgtccatcg cgaagctcgc aaaggcgcgc tggaagtcgg ccttgctgac gccgcgggac
     3121 gtgaagaacg cctcgatctt ctcgggcgtg tcgagccggt tgcccttcac gttgatctcg
     3181 cgccagatct ccgggttgag ctcgtgtttg ccgagcagct ccatcgtgta gaacacgcgc
     3241 gcgtgcgtct tcagcacgtt gttccaggtg gcgggcaggc gcacgagctc ggcattggcc
     3301 ggtcggccgt tgcggttcca ggcttcgagc ctgggttcga gcaggtagca gtggccgcag
     3361 gcgtaccaga acacctcgac gacctcgacc ttgcccggcg ccacgctcgt gggctgggcg
     3421 ggctgcagca gcgtgtagtt gacgccctgc tgcagctgga ccgccgcgga cgctgagccg
     3481 gcggccagca gcatcgcgcc ggccaggaat gcctgccgga cccggccggc cacggttcgc
     3541 gtattcatgg tggctactga ctcgcctcgg cgggcttggt tcaacg
//
LOCUS       DASPFR010000089         6419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_28389, whole genome shotgun sequence.
ACCESSION   DASPFR010000089 DASPFR010000000
VERSION     DASPFR010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28389"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..1786)
                     /locus_tag="VD737_02235"
     CDS             complement(260..1786)
                     /locus_tag="VD737_02235"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXW09407.1"
                     /translation="MLVNAGQTLTERAKLSGRMEAIVDVATGERHDFRQLNARSNQVA
                     HALLALGLAAGDRVACLMSNQARYAETYFGCAKAGYVFVALNWRLTVPELSYQLLDSG
                     ASAVVYGADQQPLVEPLIAQFPQVRWLAADHGGFDAAVRAATTAEPPIGATGEDPLFM
                     MYTSGTTGRPKGALMSHRANLSWLASMLATSDLTCEERQIIVAPMFHIAGLGLTMVAI
                     YRGMTSVIVKGFDPAQMWQLVERERITGFFAVPAMLAAMHQHPARATAKCPSLRWLLC
                     GAAPVPVSLIEAWHSMGVQVRQVYGATETHGGICLLNSEHAAAKVGSTGLPYYGIDVR
                     VVDLAGNPVPPGLPGEVITRGPHLFTGYWNRPDATRDAIRDGWFHTGDIAEVDEEGFI
                     YIKDRSKDMIISGGENVYPAEVENVLSAHPGVSEVGVIGQPSAKWGESACAVVVRAGH
                     WSGDEARLLEELRTLAQARLAKFKQPKTYMFVETLPRNPSGKILKRVLRESFPGPVPE
                     "
     gene            complement(1818..2534)
                     /locus_tag="VD737_02240"
     CDS             complement(1818..2534)
                     /locus_tag="VD737_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HXW09408.1"
                     /translation="MKYRWTVLLAIVVAGCASGPVPQVATHKLEALRADALRGLPPEE
                     TGTFRPSDLVEVKTLDPTILQDVRYATSNNFLRWPVYSEARVFLQRPAAEAVVRAHRA
                     LEAQGYGLLLFDGYRPWYVTRIFWDAVPPAQRDFVADPAEGSRHNRGCAVDLTLYHLA
                     TGREVQMPSRYDDFSEKAHPDYGGGTPAQRAARDLLRQAMEAQGFTVNDREWWHYDCR
                     DWQQYRIENVAFKDIAAARR"
     gene            complement(2531..2974)
                     /locus_tag="VD737_02245"
     CDS             complement(2531..2974)
                     /locus_tag="VD737_02245"
                     /inference="COORDINATES: protein motif:HMM:NF021049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HXW09409.1"
                     /translation="MSPERLQASLHERIPLTRALGVVVRRAESAGVVVEAPLEPNVNH
                     SGTVFGGSASAVAVLAAWALVEVRLEEAGQPARIVIRRSGMDFERPITGAFRATAQAP
                     EPHEWSRLLQSLQRGRMGRIAVRSVLTCGVERVGELEGEFAVIPP"
     gene            complement(2994..3467)
                     /locus_tag="VD737_02250"
     CDS             complement(2994..3467)
                     /locus_tag="VD737_02250"
                     /inference="COORDINATES: protein motif:HMM:NF016211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HXW09410.1"
                     /translation="MTTPAPDRSDADAGAPAPNHSRFVRGVILVVGGLALAAGVAGIF
                     LPLLPATPLLILAAACFARAHRPFHEWMLRHRWIGPMLHDWYVHRSLPYGTKTLAIVT
                     MLVSFGLSITFFVRPAWLKIVLAIVALGLATWLYRIPSRPRPARDRESADVASGR"
     gene            complement(3522..3998)
                     /locus_tag="VD737_02255"
     CDS             complement(3522..3998)
                     /locus_tag="VD737_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXW09411.1"
                     /translation="MVLGRVETPPLRGTVADLLAYKGSHVHTVARDATVYEAIAMMDA
                     RRVGALLVMEGDRLLGILSERDYTRKVILRGRASRETRVDEIMTEDVITVGAETTLGE
                     CLQLVTDRGIRHLPVTDAADRVVGLVSIGDLVRAVVAQQAETINSLKSYIGSDYPT"
     gene            complement(4016..5341)
                     /locus_tag="VD737_02260"
     CDS             complement(4016..5341)
                     /locus_tag="VD737_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXW09412.1"
                     /translation="MPNDFWYALAPDLLMLGAVVVAMALEIARVGARWSQGAFIAAAL
                     GALAVLFDQFSGQYSAMPVAGELVVDRFAIVGKAVLLACALGVAAWFPGSARGHKFWI
                     LAASSLFGGLLVLEAASFATLFLGIELLSLPAFALIVQGQGRADASEGAFKYLVLSSV
                     ASALLLFGASIGYGLTGTLALEAFAGLFATGTVQAQAALLLVLSGLFLKAAVFPFHAW
                     APDAYAAARLPVTALMASVVKATVVLAIVRIVGPATLGTAAVAVITTLALVSIAFGNL
                     AALGQARFKRLLAYSSVAHAGYMIFALLDTTGSRGVDLLWYTALYGLATVLACASFAV
                     LCPGDDDSLDRLDGAASRRPVAAAVLALSMLSLAGVPPLPGFFAKLFVFKSVIASGHT
                     AWATVAFVGSFLGLAYYVGIALRLFRPGPAPEPEPAHDRRARRHDETVA"
     gene            complement(5331..>6419)
                     /locus_tag="VD737_02265"
     CDS             complement(5331..>6419)
                     /locus_tag="VD737_02265"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HXW09413.1"
                     /translation="LVFGGPQRRRAAISFFLYTMAGSVLLLATVIFLGAEAARQTGRW
                     AFDYATLEALQLTRPQQGLVFLGIALACMVKSPLFPFHAWLPLAYTQASPSGTALMAG
                     VLSKMGAYGFLRLALPLAPEGAAAFASLFVALAAVSIFYGAVLALRQRHYKLLVAYAS
                     LSHMGYIVLGTFSLQPTGLHGAMVQILSHGVTVAGLFLLLGLLEQRRGAVWLQVHGLA
                     TRAPAFAVVLMLFVLASLALPLTSGFAAEFMVLLGAFAAGLARWQAGHGGGVLLATLL
                     AATGVVLGATYMLRFARSLLFDDSALPERLTPDLRPRELLALAPLLLLVFWIGVRPGA
                     WMERAEGTIARLAQPVAAATLGAQTHAE"
BASE COUNT         1058 a         2240 c         2266 g          855 t
ORIGIN      
        1 ccggcagcgc gagcatcgcc gctgcggcca ccagcaaggc ccccagcgca agcgccagca
       61 tggtgccgta cggccgcagg tacgggacga gcgcacgcaa tggcccgagg gaacgggact
      121 tggggcggtc ttccgtgacg acttcaggca tgcaagcagt gctagtggct agtggctagt
      181 ggttagtggc tagtgaagaa gaaggcgcat ttcactcgac gcgtattcac tagccactag
      241 ccactgacca ctgaccactt cactccggca ccggtcccgg aaagctctcc cgcagcacgc
      301 gcttcaggat cttgcccgag ggattgcgtg gcagcgtctc gacgaacatg tacgtcttgg
      361 gctgcttgaa cttggcgagc cgggcctgtg caagcgtgcg cagttcctcg agcaggcgcg
      421 cctcgtcgcc gctccagtgg cccgcgcgca cgaccacggc gcacgcgctt tcgccccact
      481 tcgcggacgg ctggccgatc acgccgacct cgctcacgcc ggggtgtgcg ctcagcacgt
      541 tctcgacttc cgcggggtac acgttctcgc cgcccgagat gatcatgtcc ttggagcggt
      601 ccttgatgta gatgaagcct tcctcgtcga cctcggcgat gtcgccggtg tggaaccacc
      661 cgtcgcggat cgcgtcgcgg gtggcgtcgg gacggttcca gtagccggtg aacaggtgcg
      721 ggccgcgcgt gatgacttcg cccggcaggc ccggtggcac cggattgccg gcgaggtcga
      781 cgacgcgcac gtcgatgccg tagtacggca gcccggtcga acccaccttg gcggcggcgt
      841 gctcggagtt cagcaggcag atgccgccgt gcgtttccgt ggctccgtac acctggcgca
      901 cctgtacgcc catcgagtgc caggcctcga tcaggctgac cggcactggg gccgcgccgc
      961 agagcagcca gcgcagtgac gggcacttcg cggtggcgcg cgccggatgc tggtgcatgg
     1021 ccgccagcat ggccggcacg gcgaagaacc ccgtgatgcg ctcgcgctcg accagctgcc
     1081 acatctgtgc cggatcgaaa cccttgacga tcaccgaggt catgccgcgg tagatcgcca
     1141 ccatcgtgag gcccaggccg gcgatgtgga acatcggcgc gacgatgatc tggcgctcct
     1201 cgcaggtgag gtcgctggtc gcaagcatgc tcgcgagcca ggagaggttg gcgcggtgcg
     1261 acatcagcgc gcctttcggc cggcccgtgg tgccgctcgt gtacatcatg aagagcgggt
     1321 cctcgccggt cgcgccgatg ggcggctcgg cggtcgtggc tgccctgacc gccgcgtcga
     1381 acccgccatg gtcggcggcc agccagcgta cctgcgggaa ctgcgcgatc agcggctcca
     1441 cgagcggttg ctggtccgca ccgtagacca cggccgacgc gccactgtcg aggagctggt
     1501 acgacagttc gggcacggtc agccgccagt tgagcgcgac gaagacgtag ccggccttgg
     1561 cacagccgaa atacgtctcg gcgtaacggg cctggttcga catcaggcag gcgacgcgat
     1621 cgccggccgc cagcccgagc gcgagcagcg cgtgggccac ctggttggag cgtgcgttca
     1681 gctgccggaa atcgtggcgc tcgccggtcg cgacgtcgac gatcgcttcc atgcggccac
     1741 tgagcttggc gcgctccgtg agcgtctgcc ctgcgttgac gagcatcgat gtcttccccc
     1801 gaagctgccg cggacgatca gcgccgtgcc gcggcaatgt ccttgaacgc gacgttctcg
     1861 atccggtact gctgccagtc gcggcagtcg tagtgccacc attcgcgatc gttgaccgtg
     1921 aacccctgtg cctccatggc ctgccgcagc aggtcacgtg cggcgcgctg cgccggcgtc
     1981 ccaccgccgt agtcgggatg cgccttctcg gaaaagtcgt cgtaacggct cggcatctgg
     2041 acttcgcggc ccgtggcgag atggtagagc gtcaggtcga cggcacaccc gcggttgtgc
     2101 ctcgaacctt ccgccgggtc cgcgacgaag tcgcgctgcg ccggcggcac ggcatcccag
     2161 aagatcctcg tgacgtacca cggtcggtac ccgtcgaaga gcagcaggcc gtatccctgc
     2221 gcttcgagcg cccggtgcgc gcggaccacc gcctcggccg ccggccgctg caggaacacg
     2281 cgggcctcgg agtacacggg ccagcgcagg aagttgttgc tcgtcgcgta gcgcacgtcc
     2341 tggaggatcg tgggatcgag cgtcttcacc tcgacgaggt cgctcggccg gaaggtgccc
     2401 gtctcctcgg ggggcagccc gcgcagggca tcggcgcgca gggcttcgag cttgtgcgtc
     2461 gcgacctgcg gcacgggtcc gctcgcgcaa ccggcgacga cgatcgcgag cagcacggtc
     2521 catcggtatt tcatggcgga atcactgcga attcgccttc cagctcaccg acgcgttcga
     2581 cgccacaggt cagcacgctg cgcacggcga tccggcccat gcgcccgcgc tgcaacgact
     2641 gcagcaggcg cgaccactcg tgtggctccg gcgcctgcgc ggtcgcccgg aacgcgccgg
     2701 tgatcggccg ctcgaagtcc atgccgctgc gacggatcac gatgcgggcc ggctgcccgg
     2761 cctcctcgag ccgcacttcg accagtgccc aggccgcgag cacggcgacc gcggacgcac
     2821 tgccgccgaa cacggtcccg ctgtggttca cgttgggctc gagcggtgcc tcgacgacga
     2881 ccccggccga ctcggcgcgc cgcacgacca cgcccagcgc gcgcgtgagc gggatgcgtt
     2941 cgtgcagcga ggcttgcagg cgttcgggcg acacgcggcc attgtgtggg tccttaccgg
     3001 ccgctcgcaa cgtccgcgga ctctcggtca cgggccgggc gcggccgcga cgggatgcgg
     3061 tacagccagg tggcgaggcc gagcgccacg atcgccagca cgatcttgag ccacgccgga
     3121 cgcacgaaga acgtgatcga caggccgaac gacaccagca tcgtcacgat ggcgagtgtc
     3181 ttggtgccgt agggaagact gcggtggacg taccagtcgt gcagcatggg cccgatccac
     3241 cgatggcgga gcatccactc gtgaaagggc cggtgggccc gggcgaaaca ggcggccgcc
     3301 aggatcagca ggggagtcgc gggcagcagg ggcaggaaga tgcccgccac gccggccgcg
     3361 agtgccaggc cgcccaccac caggatcacg ccgcgcacga accgcgaatg gttcggggcg
     3421 ggagcgccag cgtcggcgtc gctgcgatcg ggtgcgggtg tggtcacgcc ggcagcctac
     3481 cgcagctcgc cgcggcgtga ggtcagccgg cgcccacacc ctcaggtcgg atagtcgctg
     3541 ccgatgtacg acttcaggct gttgatcgtc tcggcctgct gcgccacgac cgcgcgcacc
     3601 aggtcgccga tgctcacgag gccgacgacg cggtctgctg cgtcggtgac tggcaggtgg
     3661 cgaatgcctc ggtcggtcac gagctgcagg cactcgccga gcgtggtctc ggcgcccacg
     3721 gtgatcacgt cctcggtcat gatctcgtcg acgcgggtct cacgcgaggc ccggccgcgc
     3781 aggatcacct tgcgcgtgta gtcgcgctcc gaaaggatgc ccagcaggcg gtcgccttcc
     3841 atgacgagca gtgcgccgac gcgacgcgcg tccatcatgg cgatggcctc gtacacggtg
     3901 gcgtcgcgcg ccacggtgtg cacgtgcgaa cccttgtatg ccagcaagtc cgcgacggtg
     3961 ccgcgcagcg gcggagtctc gactcttccg agcaccatgg tgcgtccctc cgttgtcagg
     4021 cgacggtctc gtcgtgccgg cgcgcgcgac ggtcgtgcgc gggctccggc tcgggcgccg
     4081 ggccgggccg gaacagccgc agcgcgatgc cgacgtaata ggcgaggccg aggaagctgc
     4141 cgacgaacgc gaccgtcgcc catgcggtat ggccggaagc gatcaccgac ttgaacacga
     4201 acagcttggc aaagaagccg ggcagcggcg ggacgcccgc gagcgacagc atcgacagcg
     4261 ccagcaccgc ggcggcgacg ggtcgcctcg aggcggcgcc gtcgagccgg tcgagcgagt
     4321 cgtcgtcacc cgggcagagc accgcgaagc tcgcgcaggc gagcacggtc gcgaggccat
     4381 agagcgcggt gtaccagagc aggtccacgc cgcggctgcc ggtcgtgtcg agcagcgcga
     4441 agatcatgta gcccgcgtgg gcgacggacg aatacgccag cagccgcttg aagcgcgcct
     4501 ggccgagcgc ggccaggttg ccgaacgcga tcgagaccag cgcgagcgtc gtgatcaccg
     4561 ccaccgcggc ggtgccgagc gtcgccggcc cgacgatccg cacgatcgcc agcacgacgg
     4621 tcgccttgac caccgaggcc atcagcgcgg tcaccggcag gcgcgccgcg gcgtaggcgt
     4681 ccggcgccca ggcgtggaac gggaagacgg ccgccttgag gaacaggccg gagagcacga
     4741 gcagcaatgc ggcctgggcc tggacggtgc cggtcgcgaa cagtccggcg aacgcctcga
     4801 gcgcgagcgt gcccgtcaag ccgtagccga tcgaggcgcc gaacaacagc agcgcgctcg
     4861 ccaccgacga gagcaccagg tacttgaagg cgccttcact cgcgtcggcg cggccctggc
     4921 cctgaacgat caacgcgaac gccggcagcg acagcagctc gatgccgagg aacagcgtcg
     4981 cgaagctcgc ggcctcgagc accagcagcc cgccgaacag cgaggaggct gcgaggatcc
     5041 agaacttgtg accgcgagcg ctgcccggga accacgcggc gacaccgagc gcgcaggcga
     5101 gcagcaccgc cttgccgacg atcgcgaagc ggtcgacgac gagttcaccg gcgaccggca
     5161 tcgcggagta ctggcccgag aactggtcga acagcaccgc gagcgcgccg agcgcggctg
     5221 cgatgaaggc gccctggctc cagcgcgcgc cgacgcgcgc gatctcgagc gccatcgcga
     5281 cgaccacggc gccgagcatc agcaggtccg gggccagcgc gtaccagaag tcattcggca
     5341 tgcgtctgcg ctccgagcgt ggccgcggca accggctgcg cgagccgggc gatcgtgcct
     5401 tcggcgcgct ccatccaggc gcccgggcgc acgccgatcc agaagaccag cagcagcagc
     5461 ggcgccaagg ccaggagctc ccgcgggcgc aggtcgggcg tgagccgctc cggcagcgcc
     5521 gagtcgtcga agagcaggct ccgcgcaaac cgcagcatgt acgttgcgcc gagcacgacg
     5581 ccagtggccg ccaggagggt ggcgagcagc acaccgccgc cgtggcccgc ctgccagcgt
     5641 gccagcccgg cggcgaacgc gccgagcagc accatgaact cggcggcgaa tccgctcgtg
     5701 agcggcagcg cgagcgaggc cagcacgaac agcatcagca cgacggcgaa ggcgggtgcc
     5761 cgcgtcgcga gtccgtgcac ctgcagccag accgcgccgc ggcgctgctc gagcagcccc
     5821 agcagcagga acagccccgc caccgtgacg ccgtggctca ggatctgcac catcgcgccg
     5881 tgcaggcccg tcggctgcag gctgaacgtg ccgagcacga tgtagcccat gtggctgagc
     5941 gaggcgtagg cgaccagcag cttgtagtgc cgttgccgca acgccagcac ggcaccgtag
     6001 aagatgctca cggcggcgag ggccacgaac agcgacgcga acgccgcagc gccttccggg
     6061 gccagcggca gcgcaagcct caggaacccg taggcgccca tcttcgacag cacgccggcc
     6121 atcagcgccg tcccgcttgg cgaggcttgc gtgtaggcga gcggcagcca cgcgtggaac
     6181 ggaaacagcg ggctcttgac catgcaggcg agcgctatgc ccaggaacac caggccctgc
     6241 tgcggccgcg tcaactgcag cgcctcgagc gtggcgtaat cgaaggccca ccgcccggtc
     6301 tgccgggccg cctcggcacc gaggaagatc accgtcgcga gcagcagtac gctgcccgcc
     6361 atcgtgtaca ggaagaaact gatcgcggca cgccggcgct gcggtccgcc gaacaccag
//
LOCUS       DASPFR010000090         2533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_516827, whole genome shotgun sequence.
ACCESSION   DASPFR010000090 DASPFR010000000
VERSION     DASPFR010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516827"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VD737_02270"
     CDS             <1..598
                     /locus_tag="VD737_02270"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXW09414.1"
                     /translation="RYPRVLERHDLDSMRALRVQVAHEHPAMRHDFTWLRLESLRRHA
                     RDAGYPETMADEGFDVFLRARNEVVLYDDVRPALDRLVGGYRLFAVSNGNANLRAIGL
                     DHYFEATLAAREAGMLKPDPRIFEILLQRAGLHARDAAHVGDDPHADVEGARAAGLLP
                     VWLNRQGVAWARESSAPSVEIRSLRELPEALPREHQGA"
     gene            834..1454
                     /locus_tag="VD737_02275"
     CDS             834..1454
                     /locus_tag="VD737_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09415.1"
                     /translation="MSGGNTIDVRNLREFFRDSVHAALERQNVGVEDHTEHYVVNLLT
                     MFARSEALFEPTADGPRLKPLAMMLADADSAASREERWRALQRVGDVSLFVAGFFAQS
                     FARRLVDVDYHVAMGGHAYGTLADTCGGGARGRALTSVFAELAQKFQRLVDALNDVSE
                     MAHRHDQRDVLRQYEIWLKTGSPRARAILANLGVDPVRVPAGRTLN"
     gene            1467..2123
                     /locus_tag="VD737_02280"
     CDS             1467..2123
                     /locus_tag="VD737_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09416.1"
                     /translation="MGLLGRLQTLLADLYDAPVEHDVRDFLITDPTHAAALQSCEEPP
                     PTDEQLLIADTHDGVAMSLYLHAEVLDHLRARCPLEALDERNLQDFCTALEGVSHFHY
                     VTWSAGCDRDVSLLELELQAEVDKYASALALMLAQRDGRFPADLFGRLFEGGRLLPHL
                     SEPERERYVEAHRFAARFCRRLEERFLRRRQARPAAMLAELRSFYRLGRHAKLRHAEG
                     "
     gene            complement(2217..>2533)
                     /locus_tag="VD737_02285"
     CDS             complement(2217..>2533)
                     /locus_tag="VD737_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09417.1"
                     /translation="EPGRLELDDRGNVTWHWSDDQDLNADDTLGAAERIRALVAPGLD
                     VQEDEEDPLSPVQNNTKGLKTGYNPYNSGALGKQSWKKKKDLRQLSKWIELKKKMEDK
                     KE"
BASE COUNT          372 a          863 c          869 g          429 t
ORIGIN      
        1 gcgctacccc cgcgtgctgg agcgccacga cctagattcg atgcgcgcgt tgcgcgtgca
       61 ggtcgctcac gagcacccgg cgatgcggca cgacttcacg tggttgcggc tcgagtcgct
      121 gcgtcgccac gcgcgcgacg cgggctaccc cgagacgatg gccgacgagg gtttcgacgt
      181 gttcttgcgc gcgcgcaacg aggtcgtgct gtacgacgat gtccggccgg cgctcgaccg
      241 gctggtcggc gggtaccgcc tgttcgccgt cagcaacggc aacgcaaacc ttcgcgcgat
      301 cgggctcgac cactacttcg aggcgacact tgccgcgcgc gaagcgggca tgctcaagcc
      361 cgacccgcgc attttcgaga tcctgctgca gcgtgcgggc ctgcacgcgc gcgacgcggc
      421 gcacgtcggc gacgacccgc acgcggacgt cgagggcgcg cgcgccgccg ggctgctgcc
      481 ggtgtggctc aaccgccagg gggtggcatg ggcgcgcgaa tcctcggcgc cgagcgtcga
      541 gatccgcagc ctgcgtgaat tgccggaggc cttgccccgc gagcaccagg gcgcgtaacg
      601 cgtctgcggc gcacgcccga cgatgccgac accggcgtgc ggcgtgcgcc ttctcacgat
      661 gccgggcgcg ccatgcccga tcgactgaga tgtcgcgcgc agaacgtgca cgttggccct
      721 tgaaatcaaa ctgcttctgt cgctagtttt cgctgcatcg gcacgttcgc ggcgaacgcg
      781 atcttgttag caggcgcgct cgctggctgc caacgcagag gagagatcgc ggcatgagcg
      841 gcgggaacac gatcgacgtg cgcaacctgc gcgagttctt ccgtgattcg gtccacgcgg
      901 cgcttgaacg ccagaacgtc ggcgtcgagg accacacaga gcactacgtg gtcaacctgc
      961 tcacgatgtt cgcgcgttcg gaggcgctgt tcgaaccgac ggccgacggt ccacggctga
     1021 agccgctcgc gatgatgctc gccgacgcgg actccgctgc gtcgcgcgag gaacgctggc
     1081 gggcgctgca acgcgtcggc gacgtgtcgc tgttcgttgc aggtttcttt gcgcagagct
     1141 tcgcgcgccg gctggtcgac gtcgactacc acgtcgcgat gggtggccac gcgtatggca
     1201 cgctggccga cacgtgcggc ggcggcgccc gcggtcgcgc gctcacttcc gtgttcgcgg
     1261 aactcgcgca gaagttccag cggctcgtgg acgcgctcaa cgacgtcagc gagatggcgc
     1321 atcgacacga ccagcgggac gtgttgcgcc aatacgagat ctggctcaag accggcagcc
     1381 cgcgggcacg cgcgatcctg gcgaacctgg gcgtggaccc ggtgcgggtg ccggccggcc
     1441 ggacgctgaa ctgagccgcc acgcgcatgg gcctgctcgg ccgactgcag acgctgctcg
     1501 ccgacctcta cgacgcgccg gtcgagcacg acgtccgcga cttcctgatc accgatccca
     1561 cgcacgcggc ggcgctgcag tcctgcgagg agccaccgcc gaccgacgag cagctgctga
     1621 tcgccgacac gcacgacggc gtggccatga gcctctacct gcacgccgag gtgctcgacc
     1681 acctgcgggc ccgctgtccg ctcgaggcgc tcgatgaacg caacctgcag gatttctgca
     1741 cggcgctcga gggcgtgagc cacttccact acgtcacgtg gagcgcgggc tgcgaccgcg
     1801 acgtgtcgct gctcgagctc gagctgcagg cggaggtcga caagtacgcg agcgcgctcg
     1861 cgttgatgct cgcccagcgc gacggccgct tcccggccga cttgttcggc cgtctcttcg
     1921 agggcggacg actgctgccg cacctgagcg agcccgagcg cgaaaggtac gtcgaggcgc
     1981 accgcttcgc ggcgcgattc tgccggcggc tcgaggaacg cttcctgcgg cgacgccagg
     2041 cgcgtcccgc cgcgatgctg gccgaactac ggtcgttcta tcggctgggc cggcatgcga
     2101 agttgcggca cgccgagggg tagtggtcag tggtcagtgg ttagtggtta gtgaaaacaa
     2161 gaaggccggg aactcccggc cttctttact agccaccagc cactagccac tagccactat
     2221 tccttcttgt cctccatctt cttcttcagc tcgatccact tggagagctg gcgcaggtct
     2281 tttttcttct tccaggactg cttgccgagc gcgccgctgt tgtaggggtt gtagccggtc
     2341 ttgagcccct tcgtgttgtt ctgcacggga ctcagcgggt cttcctcgtc ttcctggacg
     2401 tcgaggccgg gcgcgaccag cgcgcggatg cgctcggcgg cgccgagcgt gtcgtccgcg
     2461 ttgagatcct ggtcgtccga ccagtgccag gtgacgttgc cgcggtcgtc gagctcgagc
     2521 cgacccggtt cgt
//
LOCUS       DASPFR010000091         5824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_150827, whole genome shotgun sequence.
ACCESSION   DASPFR010000091 DASPFR010000000
VERSION     DASPFR010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150827"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VD737_02290"
     CDS             <1..266
                     /locus_tag="VD737_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09418.1"
                     /translation="PELPPPEIARYANETGAGVVALSVLLEDGLPQVVSQLGELRAAL
                     EPDVAIWLGGRAVQRLDQDALPPQAVVLPGTADLQQRLDTLAF"
     gene            complement(300..974)
                     /locus_tag="VD737_02295"
     CDS             complement(300..974)
                     /locus_tag="VD737_02295"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HXW09419.1"
                     /translation="MTAPRPTRHALAVAVLAGGLLQGCTSGLLESQVPAPDTYRLGQV
                     ATPVDPSAPTATSAPSSSPVLTVARPRASRALDTDRIAVVPGPRRFEYYADVRWAEPA
                     PQMLQQNLVAAFEASGRFAGTVAAPARVPSELMLDVEVRHFEAIAASPGATPEVFVQV
                     QASLVDMRRAQRLASFVSEARVAATGNRRDAIVAAFDEASARVVADVVERTATAAPAT
                     TAGSAQ"
     gene            complement(971..1906)
                     /locus_tag="VD737_02300"
     CDS             complement(971..1906)
                     /locus_tag="VD737_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HXW09420.1"
                     /translation="MERDARYATVALFALACIVAALAFVWWYSGRGDRRDFDTYEIYF
                     EGTVSGLAKGSPVRYLGVDVGRVTSLTVDRNDPGRVKVVAEIDAEAPVSGATQAKLGF
                     LGLTGLLYIDLQQNERVSASRRLPQGERYPVIAARKGSIEASAERLPEILGQAAEIMS
                     RVEGLLAEENVAGITATIANLERTTAQLPAALADAQALASDLRKISAATLALTQRADA
                     TIQAAQPELSAALANARVASEKFARTADGLDRLLNENDGAFGRSAGASVVELQQLAVD
                     ARNASIEIRELARELRERPSSLLLEQAEGGVEMPR"
     gene            complement(1896..2675)
                     /locus_tag="VD737_02305"
     CDS             complement(1896..2675)
                     /locus_tag="VD737_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXW09421.1"
                     /translation="MAARDVVISVRDIVNRFGTQTVHDGVSFDVHAGEVFGIVGGSGS
                     GKSVLLRTLLGLRQPDSGTVELEGRDLTQLSDVELRDVKRRYGVTFQHGALFTSLTVR
                     ENVILPIVEALQLEEATLRSLAELRLRMVGLPVDAGDKFPAQLSGGMVKRAALARALA
                     LDPVLLFLDEPTAGLDPIAAAAFDDLVLYLRRTLGLTVVMVTHDLDTLARTCDRVAVL
                     VEAKVIVDTLEGIVRNEHPWIREYFHGARGRLIDMEAGGGT"
     gene            complement(2675..3808)
                     /locus_tag="VD737_02310"
     CDS             complement(2675..3808)
                     /locus_tag="VD737_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXW09422.1"
                     /translation="MPWPTARDFVAQCDASTATLRLSGDCRAVDLRELERGLAEWSRA
                     GARTVDLTQAGDFDIGPAWVLERAIARARATHDHPPELVGSAPAHFAYLAELLADQPG
                     VDPDLADAEPSRPWLVRFGHSLHERFHSWYEALTYGGALIGTAGQAWRSLRRLRIPSV
                     VRHAYETGYQAVPVVAVIAFLISVISAYIGAQQLRPYGAEIFTVDLVALGVLRELGVL
                     LTAIMVAGRTGSAFAAEIGVMKLNDEIDALESMGLNPYEVLVLPRVLALVIALPLLTI
                     VADFMGLLGGGLLARYLIDLPWPQFFQRLDDAIGPTTFWAGLVKAPVFGMLIALTGTL
                     RGLQVRESSRELGRLTTVAVVQSIFLVIFADAMFAIFFLEIDF"
     gene            3855..>5824
                     /locus_tag="VD737_02315"
     CDS             3855..>5824
                     /locus_tag="VD737_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HXW09423.1"
                     /translation="MPGEFLTQILILLAGSLLVLSVVRRFRLPPILGYLVVGMLLGPH
                     SLGLVSNDESIRRLAEIGVVFLVFTLGLEFSLARMVAMKSEVLGVGGLQVLLCTAAFG
                     GMAYAAGAGPGASVVIGGALAMSSTAIVLRQLGDQLELARAHARLALGILLFQDLAFA
                     PLLGLATALGSGDVTGPLWLLGVAGRAALALLAVLVIGRLVARPLFREITRHRSTETF
                     TLTVLFVALGGAWATDELGLSMPLGGFLAGMLLAETEFKHQTEAVIKPFHDILLGVFF
                     VSVGMLLNLAELLAQLPLVLLVLVVLLATKAVVVTFVVRLFVANTRKALRTGIVVCLG
                     GEFGFALVTLLLRGDAVDPRTGQVLLTAIALSMLVGPLLVRYNKLIADTLLRREVATT
                     TDMALETVSTREVAAGHGHIIICGFGRVGQNLARIVERRGFTYVGLDADPLRVRDARM
                     AGDPVVYGDAVHPEVLRTLGLEQAAVVVLTFDAPDTAARIVRTVRGLRADVPVLVRTE
                     DDSHLDELLAAGATEVIPGTFETSLALASHLLLFLKVPPSSVLESTEEVRHERYAILR
                     SVFRRRDVPRLPEEDDPPPPREQLYTVVLPPGATAVDRSIRDVGLDHGPVTVSAIHRA
                     GVTGRSPAPETQLREGDVLVLWGRPEDLEQVE"
BASE COUNT          827 a         2058 c         2091 g          848 t
ORIGIN      
        1 ggcccgagct gcccccgccg gagatcgcac gctacgcgaa cgagaccggc gccggcgtcg
       61 tggcgctcag cgtgctgctc gaggacggac tgccgcaggt cgtgtcgcag ctgggcgaac
      121 tgcgcgcggc gctcgaaccc gacgtggcga tctggctcgg cggacgcgcc gtgcagcggc
      181 tcgaccagga cgccctgccg ccgcaggcgg tcgtcctgcc gggcacggcg gaccttcagc
      241 agcggctcga cacgctggcg ttctgacgca gggtcgccgc cgaacgcaac gctgaacgac
      301 tactgcgcgg accccgccgt cgtggccggg gccgccgtcg ccgtgcgttc gacgacgtcg
      361 gcgacgacgc gggcactcgc ctcgtcgaac gcggccacga tcgcatcgcg ccggttgccg
      421 gtcgcggcca cgcgtgcctc gctcacgaag ctcgccagcc gttgcgcgcg gcgcatgtcg
      481 accaggctcg cctgcacctg cacgaacacc tcgggtgtcg cgccgggcga ggccgcgatg
      541 gcctcgaagt gtcggacttc gacgtcgagc atcagctcgg aaggcacgcg tgccggcgcg
      601 gcgaccgtgc ctgcgaagcg gccggacgct tcgaaggctg cgaccaggtt ctgctgcagc
      661 atctgcggcg cgggctcggc ccaccgaacg tccgcgtagt actcgaaacg tcgcggtccg
      721 ggcaccaccg cgatccggtc ggtgtcgagc gccctcgacg cacgcggccg cgccaccgtc
      781 agcactggac tggacgacgg cgccgacgtg gccgtcggtg cgctcggatc gacgggcgtg
      841 gcgacctgcc ccagccggta ggtgtcgggc gccggaacct gcgactcgag caggcccgac
      901 gtgcagccct gcagcaggcc gccggcgagc acggcgacgg ccagcgcgtg acgcgtcggc
      961 ctgggcgccg tcatcgtggc atctccacgc cgccctcggc ctgctcgagc agcaggctcg
     1021 acggccgttc gcgcagttca cgcgcgagct cgcgaatctc gatgctcgcg ttgcgggcgt
     1081 cgacggcgag ttgctgcagc tccacgacgc tggcgccggc cgagcgaccg aaggcgccgt
     1141 cgttctcgtt gagcagccgg tcgaggccgt cggcggtgcg cgcgaacttc tccgaggcca
     1201 cccgcgcatt ggcgagcgcc gcgctcagct ccggttgcgc ggcctggatc gtggcgtcgg
     1261 cgcgctgggt cagcgcgagc gtcgcagcgc tgatcttccg caggtcggac gcgagcgcct
     1321 gcgcatcagc gagggccgcc ggcaactggg cggtcgtacg ttcgaggttg gcgatggtcg
     1381 cggtgatccc cgcaacgttc tcttcggcga gcagtccctc gacgcggctc atgatctcgg
     1441 cggcctggcc gaggatctcc gggagccgct cggccgaagc ctcgatgctg cccttgcgcg
     1501 cggcgatgac ggggtagcgc tcgccctgcg gcagccgccg cgacgccgaa acgcgctcgt
     1561 tctgctgcag gtcgatgtag agcaggccgg tgagcccgag gaaaccgagc ttggcctgcg
     1621 tggcgccgga gacgggcgcc tccgcgtcga tctcggcgac gaccttcacg cgacccggat
     1681 cgttccgatc caccgtcaga gacgtgacgc ggccgacgtc gacgccgagg taacgcacgg
     1741 gactgccctt ggccagcccg ctcacggtgc cctcgaaata gatctcgtag gtgtcgaagt
     1801 cgcggcgatc gccgcggccc gagtaccacc agacgaacgc gagtgccgcc acgatgcagg
     1861 cgagcgcgaa cagcgcgact gtcgcgtagc gggcatcacg ttccaccgcc cgcctccatg
     1921 tcgatcagcc ggcctcgtgc gccgtggaag tactcgcgga tccaggggtg ctcgttgcgg
     1981 acgatgccct cgagcgtatc gacgatgacc ttggcttcga ccagcaccgc gacacgatca
     2041 catgtccgtg cgagggtgtc cagatcgtgg gtgaccatga cgaccgtcag tccgagcgta
     2101 cggcgcaggt acagcacgag gtcgtcgaac gccgccgcag cgatggggtc gaggcccgcg
     2161 gtcggctcgt cgaggaacag cagcaccggg tcgagcgcga gcgcccgcgc cagcgcggca
     2221 cgcttgacca tgccccccga gagctgcgcc gggaacttgt cgccggcgtc caccggcagg
     2281 ccgaccatgc gcaggcgcag ctcggcgagg ctccgcaacg tcgcctcctc gagctgcagg
     2341 gcctcgacga tcgggaggat cacgttctcg cggaccgtga gcgacgtgaa caacgcgccg
     2401 tgctggaagg tcacgccgta ccggcgcttg acgtcgcgca actcgacgtc ggagagctgc
     2461 gtgaggtcgc ggccttcgag ctcgacggtg cccgagtcgg gctggcgcag cccgagcagg
     2521 gttctgagca gcacggactt gccgctgccc gacccgccca cgatgccgaa cacctcgccg
     2581 gcgtgcacgt cgaacgagac gccgtcgtgg accgtctgcg tcccgaagcg attgacgatg
     2641 tccctcaccg agatcacgac gtcgcgggcg gccatcagaa gtcgatctcg aggaagaaga
     2701 tcgcgaacat cgcgtcggcg aagatcacca ggaagatgga ctgcacgacg gcgacggtcg
     2761 tcaggcgtcc gagctcgcgc gaggactcgc gcacctgcag gccgcgcagc gtgcccgtca
     2821 gcgcgatcag catgccgaac acgggcgcct tgacgagccc cgcccagaac gtcgtcggcc
     2881 cgatcgcgtc gtcgagccgc tggaagaact gcggccaggg caggtcgatc aggtagcgcg
     2941 cgagcagccc gccgccgagc aggcccatga agtcggcgac gatcgtgagc agcggcagcg
     3001 cgatcaccag ggcgagcacg cgcggcagca ccagcacctc gtacgggttg agtcccatgg
     3061 actcgagcgc gtcgatctcg tcattgagct tcatgacgcc gatctcggcg gcgaaggcgc
     3121 tgccggtgcg tccggccacc atgatggcgg tgagcagcac gccgagctcg cgcagcacgc
     3181 cgagggccac gaggtcgacc gtgaagatct cggccccgta tggccgcagt tgctgcgcgc
     3241 cgatgtaggc ggagatgacg ctgatcagga aggcgatcac cgcgaccacc ggcaccgcct
     3301 ggtagccggt ctcgtaagcg tggcgcacga ccgacgggat ccgcaggcga cgcagcgagc
     3361 gccaggcctg gccggcggtc ccgatcagcg cccccccgta ggtgagcgcc tcataccagc
     3421 tgtgaaaacg ctcgtgcagc gagtgtccga aacgcacgag ccacggccgc gacggctctg
     3481 cgtcggcgag gtctggatcg acgccgggct ggtccgccag gagttcggcc aggtaggcga
     3541 aatgcgccgg cgccgacccg accagctccg gcggatggtc gtgggtggcg cgggcgcggg
     3601 cgatcgcgcg ctcgagcacc cacgccggcc cgatgtcgaa gtcgccggcc tgcgtcaggt
     3661 cgaccgtccg ggcgccggcc cgagaccact ccgcgagccc gcgctcgagc tccctcaagt
     3721 cgaccgcgcg gcagtcgccc gacagccgca gggtggccgt cgaggcgtcg cactgcgcga
     3781 cgaagtcacg ggcggtcggc catggcattg cggcgggaat gctagcatcg gcccgatggc
     3841 gacgtgaggg tcccatgccg ggcgagttcc tcacccagat cctgatcctg ctcgcgggct
     3901 cgctgctcgt cctgagcgtc gtcaggcggt ttcgcctccc gccgatcctc ggttacctgg
     3961 tggttggcat gttgctggga ccgcattcgc tgggcctggt atcgaacgac gagtcgatcc
     4021 gcaggctcgc cgagatcggc gtcgtgttcc tggtcttcac gctcggcctc gagttctcgc
     4081 tcgcccgcat ggtcgcgatg aagtccgagg tgctcggcgt cggcgggctg caggtgctgc
     4141 tttgcacggc cgcgttcggc ggcatggcct acgcggccgg cgccggaccc ggcgcgtccg
     4201 tggtgatcgg cggtgcgctc gcgatgtcgt cgacggcaat cgtgctgcgc cagctcggcg
     4261 accagctcga actcgcccgc gcgcacgcac ggctggcgct cggcatcctg ctgttccagg
     4321 acctcgcgtt cgcgccgctg ctcggcctcg cgaccgcgct cggcagtggc gacgtgaccg
     4381 gacccttgtg gctgctcggc gtggccgggc gggccgcgct cgcgctgctg gccgtgctcg
     4441 tgatcgggcg actggtcgcg cgtccgctgt tccgggagat cacccggcac cgcagcaccg
     4501 aaacgttcac gctgaccgtg ctgttcgtcg ccctcggcgg cgcgtgggcc accgacgagc
     4561 tcgggctgtc gatgccgctc ggcggcttcc tggcgggaat gctgctcgcg gaaaccgagt
     4621 tcaagcacca gaccgaggcg gtgatcaagc cgttccacga catcctgctc ggggtgttct
     4681 tcgtgtccgt gggcatgctg ctcaacctcg cggagctgct tgcgcagctg ccgctggtgc
     4741 tgctggtgct ggtggtgctg ctcgcgacca aggcggtcgt ggtcacgttc gtcgtgcggc
     4801 tcttcgtcgc caacacgcgc aaggcgctcc gcaccggcat cgtcgtctgc ctcggtggcg
     4861 agttcggctt cgcgctggtc acgctgctgc tgcgcggcga cgcggtggac ccgcgcaccg
     4921 gacaggtgct gctgacggcg attgcgctca gcatgctggt cgggccgctg ctcgtccgct
     4981 acaacaagct gatcgccgac acgctgctgc gccgcgaggt ggccacgacg acggacatgg
     5041 cgctcgagac ggtgtccacg cgcgaggtcg cggccgggca cgggcacatc atcatctgcg
     5101 gcttcgggcg ggtcggccag aacctcgcgc ggatcgtcga gcgccgcggc ttcacctacg
     5161 tggggctcga cgccgacccg ctgcgcgtgc gcgacgcgcg catggcgggc gatccggtgg
     5221 tctacggcga cgccgtgcac cccgaggtgc tgcggacgct cgggctcgag caggccgccg
     5281 tcgtcgttct caccttcgat gcgccggaca ccgcggcgcg gatcgtgcgc acggtgcgtg
     5341 gactgcgcgc ggacgtgccg gtgctcgtcc ggaccgaaga cgacagccac ctggacgagc
     5401 tgctcgccgc gggcgcgacc gaggtgatcc ccggcacgtt cgagacgagc ctcgcgctgg
     5461 cgtcacacct gcttctgttc ctgaaggtgc cgccgtccag cgtgctcgag tcgaccgaag
     5521 aggtgcgtca cgagcgctac gcgatcctgc gcagcgtctt caggcgtcgc gacgtgccgc
     5581 gactgcccga ggaggacgac ccgccgccgc cccgcgagca gctctacacg gtcgtgttgc
     5641 cgccgggcgc cacggcggtc gaccgcagca tccgcgacgt gggactcgat cacgggcccg
     5701 tcacggtcag cgcgatccac cgcgccggcg tcaccggccg ttcgccggca cccgagacgc
     5761 aattgcgcga aggcgacgtg ctcgtgctgt ggggccggcc cgaggacctc gagcaggtcg
     5821 agaa
//
LOCUS       DASPFR010000092         7651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_517588, whole genome shotgun sequence.
ACCESSION   DASPFR010000092 DASPFR010000000
VERSION     DASPFR010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7651
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517588"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..1862
                     /locus_tag="VD737_02320"
     CDS             357..1862
                     /locus_tag="VD737_02320"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09424.1"
                     /translation="MQHNDDVKELTHIVATGAPPRAAARAAKRPRDSGDAPRILLLTS
                     GLGCGHLRAAEAVERALLRIAPRATVRQLDFWTLMNPGVAAAIQNKYLELVLEHTDLY
                     ARLHGLDERTWRRVIENDIPPPAEVIELIELMTRNGDPERKSNFVEWALGPYPTDLLL
                     YPTACAALPTSSSERAGSNVALLRLALLKWAFLRLQGRMEQRLLDFGPDAIVATQMVP
                     AALVSAVKTSSRRWRGVPLVGVLTDFGAHDYWAQPGIDRYCVPHDSLAGPPLVPEGAD
                     AAGRVVVTGVPLMPGFEALPDRPEARRALGLRDDDARPVVLVLGGGLGIGLDDVVVPL
                     CRDVEDCHVAVMAGRNAELRDRLEVRCAAPHGVGRGTTVAVHGWTEHMERFLVAADVV
                     VGKSGGLTAAEVLACGRPLLVTRTLQGQESFNVRFLERHGIGRFVGIDGLAAQVRAWL
                     AHPEELRAIHARAWTAGRRDGAVQVAEQVLELAAARMTRSRGSRVVRPGVQ"
     gene            1886..2488
                     /locus_tag="VD737_02325"
     CDS             1886..2488
                     /locus_tag="VD737_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09425.1"
                     /translation="MTMPRARTALRHALERVDELYRRRFELAPVGPLLYVGLERHAGE
                     PCALHDGTRLEPGAWIGRLHFNNARAAEVEADNRPQAGIRFARLLRDSFAELAERSRN
                     DTPLREVAVFEGITWLRAHGGTVGFDARPLPHGPRRWLLGAHFRLLIWAFAPVARGSA
                     VLEVAPHRFRITRDALIASFGTRRRAPRIARPPTPWDDTT"
     gene            2485..3138
                     /locus_tag="VD737_02330"
     CDS             2485..3138
                     /locus_tag="VD737_02330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXW09426.1"
                     /translation="MSAELEPMPRDGPDVVRRIEDHSGHCYLTFDDGPHAGWTPRVLE
                     VLARADVRATFFVIGRLAQQSSALLREVRAAGHVVGNHGFSHRHPWTLTRSRARCEVR
                     TALDTIAQALGERPQWFRPPHGRLGAYLVEAAREEGQRVALWSVSAVDWGPFATTERI
                     RARLGALRAGDIVLMHDGPLRHNRPDRTLQALPTLLAALARDGPTPAPLPVVATMGA"
     gene            3135..3488
                     /locus_tag="VD737_02335"
     CDS             3135..3488
                     /locus_tag="VD737_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09427.1"
                     /translation="MNDQSAGWSPDSGDDDYDGEGHRLRSRLERTLMDRARRAAERDR
                     QKGPRRPLQHAIALAAAVVLTLVIGLGFDAFLTSVQKVLRMLDEQEALQKQQQEQVQP
                     DPSEPIPAYVVPGEE"
     gene            complement(3464..4786)
                     /locus_tag="VD737_02340"
     CDS             complement(3464..4786)
                     /locus_tag="VD737_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HXW09428.1"
                     /translation="MTRDPEAQLVRELGLRQLTAHIFNYTVGSGIFVLPAIAVAQLGT
                     AAPLAYLVCATVMALVVMVFMEAGSRVTITGGPYAYVEVGLGSFFGFVTGVLLAVATI
                     SAGGAIAALLGQTAARLLGLVGPAWSAAMTLLLVTALVAVNVRGLRWGARLVEWSTAA
                     KLVPLLAFVLVGAWFVDPGNLRIDTWPSASQVTATSGVLVFAFLGIESALGPTGEVRD
                     AARTVPRAVALALLAATLLYLAIQLVALGVLGPLLAADTVAPLASAAGTFAGDAGAAF
                     LLAGATISMLGWVTGAVLAAPRTFFALARDGFLPRQLALVHARHHTPHVAIVFYGVAV
                     ALVSLTGTFAQLAVVANLAVLAVYALGALAVLALRRKDVRSDDGVAPLRIPGGALVPV
                     LTCVLIGWIAWQTTTLREAIAFAVVWAVALPVYLWRRRRAAHSSPGTT"
     gene            complement(4790..5500)
                     /locus_tag="VD737_02345"
     CDS             complement(4790..5500)
                     /locus_tag="VD737_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09429.1"
                     /translation="MKQPPRPQIAPEAGGLTRQHLRRLRQYYRSAGWPCHDNVELDLL
                     DRGLVRRAPGEAPERIVVTDAGIGLLARQLECNRRAHAEHDALVDRVARWLLAQRRLV
                     FRGIALRTRLDERWALSKPDVFSLRHSTAARRLQPAVHEIKVRRADLLGDLKNDGKRR
                     SYQSYSQTFHYVIAEGIAEAAEIPEDCGLIVAGERSLRVVRPAPHREVSLTTAQWVAL
                     VRAGAEFGDDDEPAQMSF"
     gene            5561..6157
                     /locus_tag="VD737_02350"
     CDS             5561..6157
                     /locus_tag="VD737_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HXW09430.1"
                     /translation="MWMVRKACLGLLRLMGWRSVLIWPAERHGLILVYPHTSNWDFVI
                     GILFKVGNGLPAHWMGKDTMFRWPVGRLLRAMGGVPINRRERTGLIASLLEEFRRHDW
                     LWLAVAPEGTRSYTDHLKSGFYQIALAADLPIGLGYLDYARRCIGIDTYVRMTGDEDR
                     DLATLRAFYADKRACIPEHAGDLRFRSTAGSPREESSS"
     gene            6230..7492
                     /locus_tag="VD737_02355"
     CDS             6230..7492
                     /locus_tag="VD737_02355"
                     /inference="COORDINATES: protein motif:HMM:NF013641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09431.1"
                     /translation="MPAASSSPPVAALQPRDANRFAFVLHPLTIDYLARHPRYRWTRN
                     LPRGLVESAAAYMPAARVGRAVGGRSPSTGQPIDGLIYALGATPRQMLTRAPEFTYRR
                     LDQAVRDAADRGARIVGLGAFTKVVGDAGVTVARRAPIPVTTGNSLTIAATLETAKLT
                     ARRMGWPDLTHARAMVVGATGSIGSVCSRLLAAAVRDVVLVSIEPERLAALERTIEAE
                     TPGARVALDTTTTAHVGECDLIVTATSAFGQRVLDVSQCKPGAVILDVALPPDISAEE
                     AAVRPDVLVVESGEVLIPGPVEFSFDIGLPRGVAYACLAEAALLAMEGRFECFTLGRD
                     VDPAKVKEIYRLFRRHRFQIAPLRTFGVPLTDALVEEKNARAEELRRDRARFERVKAD
                     AAAKIARIPPRAKGVAGTQPPPPRQRAA"
BASE COUNT         1094 a         2761 c         2741 g         1055 t
ORIGIN      
        1 tcaccaccag tttcgatccg cggccgcgcg aggtcgccgg tcatgatgtt tgccgcggcc
       61 ggtcacgagg cggagaaccg cacgcgaaaa atggggacac tccggcattc ccgttaattg
      121 gggacactcc tgatttcaac gtcgcgcaac tgcccttttc ctcggcactt gcgccacgac
      181 ggtgcactgc gaccggacgt cgccttgcct cgcgagagcg gtctcacggc cgcggcgcgt
      241 ttattcagga gtgtccccaa ttaacgggaa attcggagtg tccccagatc cggcgggtgg
      301 tagaactcgt ctgacagtac atccgccgcg cccgctgcgg gacgccggac gcggccgtgc
      361 agcacaatga cgacgtgaag gaactcacgc acatcgtcgc gacgggcgca ccgccgcgcg
      421 cggcagcgcg cgccgcaaaa cgtccgcggg attccggcga tgcgccgcgc atcctgttgc
      481 tgacgtcggg tctcggctgc ggccacctgc gcgccgccga ggcggtcgag cgcgcgttgc
      541 tgcggatcgc cccgcgcgcg acggtgcggc agctcgactt ctggacgttg atgaacccgg
      601 gcgtcgccgc ggcgatccag aacaagtacc tcgaactcgt gctcgagcac accgacctct
      661 acgcgcgcct gcacggcctc gacgagcgca cgtggcggcg cgtgatcgag aacgacatcc
      721 cgccgccggc cgaagtcatc gagctgatcg agctgatgac gcgcaacggc gaccccgaac
      781 gcaagtccaa cttcgtcgag tgggcgctcg gcccgtatcc gaccgacctg ctgctgtatc
      841 cgaccgcctg cgccgcgctg ccgacgagtt cgagcgaacg cgccggcagc aacgtcgcgc
      901 tgttgcggct cgcgctgctc aagtgggcgt tcctgcgcct gcagggccgc atggaacagc
      961 ggctgctcga cttcggtccc gacgcgatcg tcgccacaca gatggtgccg gcggcgctgg
     1021 tgtcggccgt caagacgtcg tcgcgtcgct ggcgcggcgt gccgctcgtc ggcgtgctga
     1081 ccgacttcgg cgcgcacgac tactgggcgc agcccgggat cgaccgctac tgcgtccccc
     1141 acgattccct cgccggtccg ccgctcgtac cggagggcgc cgacgccgcc ggccgcgtcg
     1201 tcgtgaccgg cgtgccgctg atgccgggat tcgaggcgct gcccgaccgt cccgaggcgc
     1261 gtcgcgcgct cgggctgcgc gacgacgacg cgcgcccggt cgtgctggtg cttggcgggg
     1321 gtctcggcat cgggctcgac gacgtggtcg tgccgctctg tcgggacgtg gaggactgtc
     1381 acgtggccgt gatggccggt cgcaatgccg agctgcgcga tcgcctcgag gtccgatgcg
     1441 cggcgccgca cggcgtgggt cgcgggacca cggtcgccgt gcacggctgg accgagcaca
     1501 tggagcgttt cctggtcgcg gcggacgtcg tggtgggcaa gtccggcggc ctcacggccg
     1561 ccgaggtgct cgcctgcggc cgtccgctgc tcgtgacccg cacgctgcag ggacaggaga
     1621 gcttcaacgt acggtttctc gagcgccacg gcatcgggcg gttcgtcggc atcgacgggc
     1681 tcgcggcgca ggtgcgggcg tggctcgcgc acccggagga gctgcgcgcg attcacgcgc
     1741 gcgcgtggac ggccggccga cgcgacggcg ccgtgcaggt cgccgagcag gtgctcgagc
     1801 tcgcggcagc gcgcatgacg cgctcgcgcg ggtcgcgcgt agtgcgcccg ggggtgcagt
     1861 gatggtgccg gccacgcgag ccgccgtgac catgccccgc gcgcggaccg cgctccgcca
     1921 cgcgctcgag cgcgtcgacg agctctaccg tcgccgcttc gagctcgcgc cggtgggtcc
     1981 actgctttac gtcgggctcg agcgccacgc gggcgagccc tgcgcgctgc acgacggcac
     2041 gcgtctcgag ccaggcgcct ggatcggtcg cctgcacttc aacaacgcgc gcgccgccga
     2101 agtcgaggcg gacaaccggc cgcaggcggg catccggttc gcgcggctgc tgcgcgattc
     2161 gttcgccgag cttgcggagc gctcgcgcaa cgacacgccg ttgcgagaag tcgccgtgtt
     2221 cgaaggcatc acgtggctgc gcgcgcacgg cggcacggtc ggcttcgacg cacggccact
     2281 gcctcacggg ccgcggcgct ggctgcttgg tgcgcacttc cgcctgctga tctgggcctt
     2341 tgcacccgtg gcgcgcggct cggccgtgct cgaagtggcg ccgcaccgtt tccggatcac
     2401 ccgcgacgcg ctgatcgcca gcttcggcac gcggcggcgg gccccgcgga tcgcgcgtcc
     2461 ccccacgccc tgggacgaca cgacgtgagc gcggagctgg agccgatgcc gcgcgacggg
     2521 cccgatgtcg tgcgtcgaat cgaggaccac tccggccact gctacctcac gttcgacgac
     2581 ggaccgcacg ccggctggac gccacgcgtg ctcgaggtcc tcgcgcgagc cgacgtgcgc
     2641 gccacgttct tcgtgatcgg ccgtctcgca cagcagtcgt cggcgctgct gcgggaggtc
     2701 cgcgcggccg gtcacgtggt cggcaaccac ggcttcagcc accgtcatcc gtggacgctc
     2761 acgcgttcgc gtgcgcgctg cgaggtccgc accgcgctcg acacgatcgc ccaggcgctg
     2821 ggcgagcgtc cgcagtggtt ccggccgccg cacggacgcc tcggcgcgta cctcgtggag
     2881 gcggcgcggg aggaaggaca acgcgtggcg ctgtggagcg tcagcgcggt cgactgggga
     2941 ccgttcgcga cgaccgagcg catccgggcg cgcctcggcg cgctgcgcgc cggcgacatc
     3001 gtgctcatgc acgacggacc gctgcggcac aaccgtcctg accgcacgct gcaggcgttg
     3061 ccgaccctgc tggctgcgct cgcgcgcgac gggccgacac ccgcgccgct gccggtcgtc
     3121 gctacaatgg gtgcatgaac gaccaatccg cagggtggtc acccgactcc ggcgacgacg
     3181 actacgatgg cgagggccat cggctgcgct cacggctgga acgcacgctc atggatcgcg
     3241 cgcgccgcgc ggcggagcgc gaccggcaga aaggaccgcg tcggccactt cagcacgcga
     3301 tcgcgctggc ggccgccgtg gtgctgacgc tggtgatcgg gctggggttc gatgcattcc
     3361 tgaccagcgt gcagaaggtc ctgcgcatgc tcgacgagca ggaggcgctg cagaagcagc
     3421 agcaggagca ggtgcagccg gatccgtcgg agccgatccc tgcctacgtc gtgcccggcg
     3481 aggagtgagc cgcccgtctg cggcgccaca ggtataccgg caacgcaacg gcccagacga
     3541 ccgcgaatgc aattgcttcg cgcagggtcg tcgtctgcca ggcgatccag ccgatcagca
     3601 cgcaggtgag cacgggcacc agcgcaccgc ccgggatgcg cagcggtgcg acgccgtcgt
     3661 cactgcgcac gtccttgcgc cgcagcgcaa gcacggccag cgcgcccagc gcgtagaccg
     3721 cgagcaccgc gaggttcgcg acgacggcca gctgcgcgaa ggtgccggtc aacgagacca
     3781 gcgcgacggc aacgccatag aagacgatcg cgacgtgcgg cgtgtgatga cgcgcgtgca
     3841 cgagcgcgag ctggcgcggc aggaaaccgt cgcgcgccag cgcgaagaac gtgcgcgggg
     3901 cggccagcac cgccccggtg acccatccga gcatcgagat cgtggcgccg gcgagcagga
     3961 acgccgcgcc tgcgtcgccg gcgaacgtgc cggccgcgct ggcgagcggc gcgaccgtgt
     4021 ccgccgcgag cagcggcccc agcactccga gcgccacgag ctggatcgcg aggtacagca
     4081 gcgtcgccgc cagcagcgcg agcgcgaccg cgcgtggcac ggtgcgtgcg gcatcccgca
     4141 cctcgccggt cggtccgagc gcggattcga tgcccaggaa cgcgaacacc agcacgccgc
     4201 tcgtcgccgt gacctgcgac gccgacggcc aggtgtcgat gcgcaggttg ccgggatcca
     4261 cgaaccacgc gccgaccagc acgaacgcga gcagcggcac cagcttcgcc gccgtcgacc
     4321 attcgaccag gcgcgcgccc cagcgcaggc cacgcacgtt gacggcgacg agcgccgtga
     4381 ccagcagcag cgtcatcgcg gcggaccacg ccgggccgac gagcccgagc agtcgcgccg
     4441 cggtctgtcc gagcagggcg gcgatggcgc cgcccgcgct gatcgtggcg accgcgagca
     4501 gcacgccggt cacgaacccg aagaacgacc cgagcccgac ctcgacgtag gcatacgggc
     4561 cgcccgtgat ggtcacgcga ctgccagctt ccatgaacac catcacgacg agtgccatca
     4621 ccgtggcgca cacgaggtag gcgagcgggg cggccgtgcc gagttgcgcg accgcgatcg
     4681 ccggcagcac gaaaatgccg ctgcccacgg tgtagttgaa gatgtgcgcg gtcagctgcc
     4741 gcaggccgag ctcgcgtacc agctgcgctt ccggatcgcg ggtcatggct cagaagctca
     4801 tctgcgccgg ctcgtcgtcg tcgccgaact cggcgcccgc gcgcaccagc gcgacccact
     4861 gcgccgtcgt gagcgacacc tcgcgatgcg gcgcgggccg cacgacgcgc agcgaccgct
     4921 caccggcgac gatcaatccg cagtcttccg ggatttccgc ggcttcggct atgccctcgg
     4981 cgatcacgta gtgaaacgtc tgcgagtacg actggtagct gcgccgcttg ccgtcgttct
     5041 tgaggtcgcc gagcaggtcc gcgcgacgca ccttgatctc gtgcacggcc ggctgcagcc
     5101 ggcgcgccgc ggtcgagtgc cgcagcgaga acacgtcggg cttcgacagc gcccagcgct
     5161 cgtcgagccg ggtgcgcagc gcgatgccgc ggaacaccag ccgcctctgc gcgagcagcc
     5221 agcgcgcgac gcggtcgacc agcgcgtcgt gttcggcgtg ggcgcggcgg ttgcactcga
     5281 gctgcctggc cagcagcccg atgccggcgt cggtcacgac gatgcgctcg ggcgcctcgc
     5341 cgggcgcgcg ccgcaccagc ccccggtcga gcaggtcgag ctcgacgttg tcgtggcacg
     5401 gccacccggc cgaccggtag tactgccgca gccggcgcag gtgctggcgg gtgagaccgc
     5461 ccgcctcggg cgcgatttgc ggtcgaggtg gttgtttcat ccctggagcc tactgcccgc
     5521 tgtgctacaa cgcggcacct cggcgaaccg gagcgtgacg atgtggatgg tgcgcaaggc
     5581 ctgtctcggg ctgctgcggc tgatgggctg gcgcagtgtg ctcatctggc cggcggagcg
     5641 ccacgggttg atcctggtct acccccacac gtccaactgg gacttcgtga tcggcatcct
     5701 gttcaaggtg ggcaacggcc tgccggccca ctggatgggc aaggacacca tgttccggtg
     5761 gccggtcggc cgcctgctgc gggcgatggg cggcgtgccg atcaatcgcc gcgagcgaac
     5821 cgggttgatc gcgtcgctgc tcgaggagtt caggcggcac gactggctgt ggctcgccgt
     5881 ggcgcccgag ggcacccggt cctacaccga ccacctgaag tccggcttct accagattgc
     5941 gctggcggcg gacctgccga tcggcctggg ctacctcgac tacgcgcggc gctgcatcgg
     6001 catcgacacc tacgtgcgca tgaccggcga cgaagaccgt gacctcgcca cgctgcgggc
     6061 gttctacgcc gacaagcgag cgtgcatccc ggagcacgcc ggggatttgc gcttccggtc
     6121 cacggcggga tccccgcggg aagaatcgtc gtcctaggga gtgttacgaa tgggcgtgcg
     6181 cgccgcgccg cccaagaatg cagtctcgac acgcaacagg tcgacgcctg tgcccgccgc
     6241 ctcgtccagt ccgccggtcg ctgccctgca gccacgtgac gccaaccgct tcgcgttcgt
     6301 gctgcacccg ctcacgatcg attacctcgc caggcatcca cgctaccgct ggacgcgcaa
     6361 cctgccgcgc ggcctggtcg agtccgccgc ggcgtacatg ccggcggctc gcgtcggccg
     6421 cgccgtcggg ggccgatcgc cgtccactgg gcagccgatc gacgggctga tctacgcgct
     6481 cggcgcgacg ccccggcaga tgctcacgcg tgcgccggag ttcacgtacc ggcggctcga
     6541 ccaggccgtg cgcgacgcgg ccgatcgcgg tgcccgcatc gtggggctcg gggcgttcac
     6601 caaggtcgtc ggcgatgccg gcgtgacggt cgcgcgccgc gcgccgattc cggtcacgac
     6661 cggaaacagc ctcacgatcg ccgcgacgct cgaaaccgcg aagctcacgg cgcgtcgcat
     6721 gggctggccg gaccttacgc acgcgcgtgc gatggtggtc ggtgcgacgg gctcgatcgg
     6781 ctcggtgtgc tcccgcctgc tcgccgccgc ggtccgcgac gtcgtgctgg tgtcgatcga
     6841 accggagcgg ctcgcggcgc tcgagcgcac gatcgaggcg gagaccccgg gcgcgcgcgt
     6901 cgcgctcgac acgaccacga cggcgcacgt cggcgaatgc gacctgatcg tcacggccac
     6961 ctccgccttc ggccagcgcg tgctcgacgt gagccagtgc aagcccggcg ccgtgatcct
     7021 cgacgtcgcg ctgccgccgg acatctcggc cgaggaagcg gcggtgcgcc cggacgtgct
     7081 ggtggtcgag agcggcgagg tgctgatccc gggaccggtc gagttcagct tcgacatcgg
     7141 cctgccgcgc ggcgtcgctt acgcctgcct ggccgaagcg gccctgctgg cgatggaggg
     7201 acgcttcgag tgcttcacgc tcgggcgcga cgtcgacccg gcgaaggtca aggagatcta
     7261 ccggctgttc cgtcgccacc gcttccaaat cgcgccgctg cgcacgttcg gcgtgccgct
     7321 caccgacgca ttggtggagg agaagaacgc gcgcgccgag gaactgcggc gcgaccgtgc
     7381 gcggttcgaa cgcgtcaagg ccgacgccgc ggcgaagatc gcccggatcc cgccgcgcgc
     7441 gaaaggcgtc gccggcacac agccgccacc gccgcggcaa cgggccgcct gagcggctcg
     7501 cgggccatcg aggcgtgcac tagcgccgca gagggctgcg caccgcatcg gacgcgggct
     7561 acgtcgtcca atcgcgccac atcggccgca gcgggctgcc cgccgcattt cgagccgggg
     7621 agacgccgta agagcggtcc ctcgcggctc g
//
LOCUS       DASPFR010000093         3526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1127949, whole genome shotgun sequence.
ACCESSION   DASPFR010000093 DASPFR010000000
VERSION     DASPFR010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127949"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /gene="murI"
                     /locus_tag="VD737_02360"
     CDS             <1..717
                     /gene="murI"
                     /locus_tag="VD737_02360"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HXW09432.1"
                     /translation="KSPGSIRQYALQAARRLHDHGVKCLVVACNTASAIALDVLTQEF
                     APVPVVGVLEPGAAAACRATRSGRIAVLATESTVRGGAYQAAIARRRPDAIVTARACP
                     LFVALAEEGWTDGPVVDGVIHRYLDDVFADAAPGTRPDTLLLGCTHFPVLTPALARVL
                     GPEVAIVDSAATTAVALEEALAARGLARRASRGERRVRLLATDGAERFARVGGVFLGR
                     SLAAGDVEIVDLAPAAASVG"
     gene            complement(706..1731)
                     /locus_tag="VD737_02365"
     CDS             complement(706..1731)
                     /locus_tag="VD737_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HXW09433.1"
                     /translation="MTTDVLLFPFGEYWWFYAGFTLFVLVVLALDLGVFHREAHEVSA
                     REAAAWSTVWVALALLFNYLFYRYMLWHFPQVADLAGLDHAALARDAGLEFLAGYVVE
                     KALAVDNVFVFVAVFSYFAIPPRLQHRVLFFGILGALVFRAVFISIGSVLMQYHWIVV
                     IFGAFLIVTGIKILFLPEKPIEPEKNPIIRLVRRFVPIWPKIEGDSFWIHREGVRYAT
                     PLFVALVFIEFSDIIFAIDSVPAIFALTNEPLIVFTSNIFAILGLRAMYFLLASAVQY
                     FQYLKYGLGIILVFVGLKMVWLNQAFGGKFPITWSLGIIGALLAVSMLVSVAARRDGR
                     AEQHSAD"
     gene            complement(1813..2835)
                     /locus_tag="VD737_02370"
     CDS             complement(1813..2835)
                     /locus_tag="VD737_02370"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXW09434.1"
                     /translation="MDTRRLGRQGLEVSALGLGCMGMTAFYRTGADPAECEATLLEAV
                     DRGVTLFDTAEVYGPYTNECLLGRVLGGARRDRVVLATKFGFELGDQATPIRGLDSRP
                     EHVREVVEQSLVRLQTDRIDLLYQHRVDPAVPIEDVVGAMAALVQAGKVRYLGLSEAS
                     PATLRRAHSVHPISALQSEYSLWERGVERSVLPACRELGIGLVPYSPLGRGFLAGQRV
                     SATDLPEGDARRGHPRFQPGNYAANLRLLEKLEDVAGRHGATPGQVALAWLLAQGSDV
                     VPIFGTTRRERLRENLGAVAVRLASGDQFLLDQVFAPGSVAGDRYPEAALAMLDRDEG
                     DAESGG"
     gene            2934..3019
                     /locus_tag="VD737_02375"
     tRNA            2934..3019
                     /locus_tag="VD737_02375"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2968..2970,aa:Tyr,seq:gta)
     gene            3086..3159
                     /locus_tag="VD737_02380"
     tRNA            3086..3159
                     /locus_tag="VD737_02380"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3118..3120,aa:Gly,seq:tcc)
     gene            3193..3268
                     /locus_tag="VD737_02385"
     tRNA            3193..3268
                     /locus_tag="VD737_02385"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3226..3228,aa:Thr,seq:ggt)
     gene            3394..>3526
                     /locus_tag="VD737_02390"
     CDS             3394..>3526
                     /locus_tag="VD737_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXW09435.1"
                     /translation="MAKEKFARTKPHVNVGTIGHVDHGKTTLTAALTKVMAETHGGTF
                     "
BASE COUNT          604 a         1184 c         1230 g          508 t
ORIGIN      
        1 aagagcccgg gctcgatccg gcagtacgcc ctgcaggcgg cgcgacgcct gcacgatcac
       61 ggcgtcaagt gcctggtggt ggcgtgcaac acggcctccg ccatcgcgct cgacgtgctc
      121 acgcaggagt tcgcgcccgt gcccgtggtc ggcgtgctcg agcccggcgc cgccgcggcg
      181 tgccgtgcga cgcgcagcgg tcgcatcgcg gtgctcgcca ccgagagcac cgtgcgcggc
      241 ggcgcgtacc aggccgccat cgcgcggcgc cggccggacg ccatcgtgac ggcgcgcgcg
      301 tgcccgctgt tcgtggcgct ggccgaggaa ggctggaccg acggcccggt ggtcgacggc
      361 gtgatccacc gctacctcga cgacgtgttc gctgacgcgg cgccggggac gcggccggac
      421 acgctgctgc tcggctgcac gcacttcccg gtgctcacac cggcgctcgc gcgggtgctc
      481 gggcccgagg tggccatcgt cgattcggcg gcgaccaccg ccgtcgcgct cgaggaggcg
      541 ctcgccgcgc gagggctcgc gcgacgcgcg tcacggggcg agcgccgcgt gcgcctgctc
      601 gcgacggacg gcgcggagcg gttcgcgcgc gtcggcggcg ttttcctcgg acgcagcctc
      661 gcggccggcg acgtcgagat cgtcgacctc gcgccggccg ccgcgtcagt cggctgagtg
      721 ctgctcggcg cgcccgtcgc gacgggccgc gacggacacc agcatcgaca cggcgagcag
      781 cgcgccgatg atgccgagcg accaggtgat cgggaacttg ccgccgaacg cctggttcag
      841 ccacaccatc ttcaggccca cgaacaccag gatgatgccg aggccgtact tgaggtactg
      901 gaagtactgc accgcgctcg cgagcaggaa gtacatcgcg cgcagcccga ggatcgcgaa
      961 gatgttcgag gtgaagacga tcagcggctc gttggtcagc gcgaagatgg ccggcaccga
     1021 atcgatcgcg aagatgatgt ccgagaactc gatgaacacg agcgccacga agagcggagt
     1081 ggcatagcgc acgccctcgc ggtggatcca gaacgagtca ccttcgatct tcggccagat
     1141 cggcacgaag cgacgcacca gcctgatgat cgggttcttc tccggttcga tcggcttctc
     1201 gggcagaaac aggatcttga tcccggtgac gatgaggaac gcgccgaaga tgaccacgat
     1261 ccagtggtac tgcatcagca ccgagccgat cgagatgaac accgcgcgga acacgagcgc
     1321 gcccaggatg ccgaagaaga gcacgcgatg ctgcaggcgc ggcggaatcg cgaagtacga
     1381 gaagacggcc acgaacacga acacgttgtc gaccgcgagc gccttctcga ccacgtaccc
     1441 ggcgaggaac tcgagccccg cgtcgcgcgc gagcgcggcg tgatccagtc cggccaggtc
     1501 cgccacctgg ggaaagtgcc agagcatgta gcggtagaag aggtagttga acagcagcgc
     1561 gagcgccacc cataccgtgc tccacgcggc cgcctcccgc gcgctgacct cgtgcgcctc
     1621 gcgatggaac acgccgaggt cgagggccag gaccaccagc acgaagagcg tgaagccggc
     1681 gtagaaccac cagtactcgc cgaaggggaa cagcagcacg tcagtcgtca tcaggccctc
     1741 tgcaagctcg gctgagtcgg cttcggtgcc cggccggttt cgccattcta gaattcgtct
     1801 attcgcgggg catcagccgc ccgattcggc gtcgccctcg tcgcggtcga gcatggccag
     1861 ggcagcttcg gggtaccggt ctcccgcgac gctccccggc gcgaagacct ggtccagcag
     1921 gaactggtcg ccgctggcga ggcggactgc cacggcgccg aggttctcgc gcaggcgctc
     1981 gcggcgcgtg gtgccgaaga tcggcacgac gtcgctgccc tgggccagca gccatgcgag
     2041 ggccacctgc ccgggtgtcg ccccgtggcg gcctgccacg tcctcgagtt tctcgagcag
     2101 ccgcaggttg gcggcgtaat tgccgggctg gaagcgcggg tggccgcgcc gcgcgtctcc
     2161 ctcaggcagg tccgtcgcgg aaacgcgctg gccggccagg aagccccggc cgagggggga
     2221 gtacgggacc aggccgatcc cgagctccct gcacgccggc agcacgctgc gctcgacgcc
     2281 gcgttcccag agcgagtact cggactgcag cgcactgatc gggtgcaccg agtgggcccg
     2341 gcgcagggtg gccgggctcg cctcggaaag gccgaggtat cggaccttgc cggcctggac
     2401 cagggccgcc atggcgccca cgacgtcctc gatcggcacc gccgggtcga cccggtgctg
     2461 gtagagcagg tcgatacgat cggtctgcag gcgcaccagc gactgctcga ccacctcgcg
     2521 gacgtgctcc ggccggctgt cgaggccgcg gatcggggtc gcctggtcgc ccagttcgaa
     2581 cccgaacttg gtcgccagca cgacgcggtc ccggcgcgct ccgccgagca cacggccgag
     2641 caggcactcg ttggtgtagg ggccgtacac ctcggccgtg tcgaacagcg tgacgccccg
     2701 gtccaccgcc tcgagcagcg tggcctcgca ctcggccggg tcggcgcccg tgcgatagaa
     2761 ggccgtcatc cccatgcagc ccaggcccag cgcggaaacc tcgaggccct gccggccgag
     2821 tcggcgtgtg tccatcggtg agtcgaactc caggtccggt acgggtggtc gggatgtcgg
     2881 atcagattga cacggccgag ccgggccccg agaatacgcg gctctctgtt cgcggagggg
     2941 tacccaagcg gccaacggga gcagactgta aatctgccgg cttatgcctt cgaaggttcg
     3001 aatccttccc cctccaccag cagacagaga ttaacggaga ggcgtggata tcgcgacacg
     3061 tgggcaccga gaaccggcga gcgctgcggg tgtagttcaa tggtagaacc tcagccttcc
     3121 aagctgttga cgtgggttcg attcccatca cccgctccaa ccggaagcct gctgggacgg
     3181 tttgcaaggc gagcccacgt agctcagtcg gtagagcacg tccttggtaa ggacgaggtc
     3241 agcggttcga tcccgctcgt gggctccagt tcgagaagtg gtcagtggtc agtggctagt
     3301 ggtcagtgaa gaacgacatc gagttctcgc ggcacctcct cactaaccac tgaccactag
     3361 gcaccagtca cttgtattca gaggtatcgg gtcgtggcta aggaaaaatt cgcacgcacg
     3421 aagccgcacg tgaacgtggg cacgattggt cacgttgacc atggcaagac gacgctgacg
     3481 gcggcgttga cgaaggtgat ggcggagacg cacggcggca ccttca
//
LOCUS       DASPFR010000094         3090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1371568, whole genome shotgun sequence.
ACCESSION   DASPFR010000094 DASPFR010000000
VERSION     DASPFR010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371568"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1408)
                     /locus_tag="VD737_02395"
     CDS             complement(122..1408)
                     /locus_tag="VD737_02395"
                     /inference="COORDINATES: protein motif:HMM:NF023330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HXW09436.1"
                     /translation="MFATPEHPLRQALNAELHARQPEALVAPLRLTFLALYSDAALRV
                     AELEHVRQLARRHGAPPPPDGTAHYSADLGRFRLKFERHSEFTRYKFIVAGEADEPFE
                     DTALRALPEDWLAQLPGRLMVATHVVLLRATGPDTPSADALASRHFAGNALVGAGIAG
                     GAGFAFTDYRLHDGFGRMVVVDRGMSERQAGRMVQRLLEIDSYRLMALLALPVAQELT
                     PWLTQAEHELADVSASLVTASESNEPSLLDQLTRLEAQIESRESQHHYRFTAAQAYHG
                     LVRRRLEELREVRIDGVQNFEEFTERRLGPAMNTCRSVWARLESLSQRVARATQLLST
                     RVDISRERQNQQLLESMNRRSAAQLRLQQTVEGLSVAAITYYIVGLVGYAAKGLHAVG
                     LHVSVEAVTAVSIPVVAVAVAYGVRHARRIVSHAVQ"
     gene            complement(1492..1998)
                     /locus_tag="VD737_02400"
     CDS             complement(1492..1998)
                     /locus_tag="VD737_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXW09437.1"
                     /translation="MGGILGLCGVALIFWPEFGRAAGRAGAALGAVFTAGSVLLSAVG
                     SLTASRNRINGLPFWPALGFGMLYGAGITVVVALAQGLSFELPAALTWWAALFYLALA
                     GSVLTFACFLTLQERIGPGPTASIGVMTPLLALVVSVAFEGFRPDVLTIVGAALAVIG
                     NVLMLRRA"
     gene            2525..>3090
                     /locus_tag="VD737_02405"
     CDS             2525..>3090
                     /locus_tag="VD737_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09438.1"
                     /translation="MLHRTKSSRPLLVALLMAAEACGHAPTDSPARLGSLPLDPRAVT
                     LSGLSSGGAMAVQFHTAHSRLVQGVGVFAAPPYYCAEGAIGNALGRCMKDGTSIPVER
                     LLQQADRWSQEGLVDPADGVAQDKVWLYRGAADPHVHVTVADALEGYYRERGVSSANL
                     VRIEREGAGHNMPIAAPGAAPCAAGEPP"
BASE COUNT          481 a         1101 c         1098 g          410 t
ORIGIN      
        1 atcgtgcgcc gggacaacga tactgccgag atccatctgc acgggcgccc cgacgtgctg
       61 ccggtgagcc gcagtcgcct gcatctgttc cgccagatgt agggccgcac cggccgaggc
      121 atcattgcac ggcgtgcgac acgatccgcc gcgcgtgccg gacgccgtac gcgaccgcga
      181 cggccacgac ggggatgctg accgccgtca cggcttccac gctgacgtgg aggccgaccg
      241 cgtgcagtcc cttggccgcg tagccgacca gccccacgat gtaataggtg atcgccgcca
      301 cggacaggcc ctcgaccgtc tgctgcagcc gcagctgcgc ggccgagcgg cgattcatgg
      361 actcgagcaa ctgctggttc tggcgctcgc gggaaatgtc gacgcgcgtg gacaggagct
      421 gcgtggcacg tgcgacgcgc tgtgacagcg actcgagccg tgcccacacg gaccggcagg
      481 tgttcatcgc gggcccgagc cggcgctcgg tgaactcctc gaagttctgc accccgtcga
      541 tgcgcacctc gcgcaactcc tcgaggcgcc ggcgcacgag gccgtgatag gcctgcgcgg
      601 ccgtgaagcg gtaatggtgc tgggactcgc ggctctcgat ctgcgcctcg agccgcgtga
      661 gctggtcgag cagcgacggc tcgttcgact cgctggccgt gacgagcgac gcgctcacgt
      721 cggcgagttc gtgctcggcc tgcgtgagcc agggtgtcag ctcctgcgcg accggcagcg
      781 cgagcagcgc catgagccgg tacgagtcga tctcgagcag gcgctgcacc atccgcccgg
      841 cctgccgttc ggacatgccg cgatcgacca ctaccatgcg cccgaacccg tcgtggagcc
      901 ggtaatcggt gaacgcgaac ccggcaccgc cggcgatgcc ggcgccgacg agcgcgttgc
      961 ccgcaaagtg acgcgaggcc agcgcgtcgg cgctcggcgt gtccggcccg gtggcccgca
     1021 gcaggaccac gtgggtcgcg accatcaacc tgccgggcag ctgggcgagc cagtcttccg
     1081 ggagcgcacg cagcgccgtg tcctcgaacg gctcgtccgc ctcaccggcc acgatgaact
     1141 tgtagcgcgt gaactccgag tgacgttcga acttgagccg gaagcggccg aggtcggcac
     1201 tgtagtgcgc tgtcccgtcc ggcggcggcg gcgcgccgtg acgacgcgcc agctggcgca
     1261 cgtgctcgag ctcggccacg cgcagggccg cgtccgagta cagggccagg aacgtcagcc
     1321 gcagcggcgc caccagcgcc tccggctggc gtgcgtgcag ctcggcgttc agcgcctgtc
     1381 gcagcgggtg ttcgggcgtg gcaaacatcg ggcggctgcc gtcgcgggcg cagtcctctg
     1441 gtcgatgcgt ggcgcccact atagcggctc gcgtgctgcg cgcgcgtcga gtcaggcgcg
     1501 tctcagcatc agcacgttac cgatcactgc cagcgcggcg cccacgatcg tcagcacgtc
     1561 cggccggaag ccctcgaagg ccaccgacac caccagcgcc agcagtggcg tcatgacccc
     1621 gatcgacgcc gtggggcccg ggccgatgcg ctcctgcagc gtcaggaagc aggcgaacgt
     1681 gagcacggaa ccggcgagcg ccaggtagaa gagcgccgcc caccacgtca acgccgccgg
     1741 cagctcgaag ctcaggccct gcgcgagcgc gaccaccacg gtgattccgg cgccgtagag
     1801 catcccgaac cccagtgccg gccagaacgg caggccgttg atgcggttgc ggctcgcggt
     1861 gaggctgccg accgccgaca gcagcaccga gcccgccgtg aacacggccc cgagcgccgc
     1921 gccggcgcga cccgcggcgc gcccgaactc cggccagaag atcagcgcga cgccgcagag
     1981 gccgagtatt ccgcccaccc agaaacgggg ccgtgcctcc gtgccgaaga gcgcgcgcgc
     2041 cgagtcccgt cacgagcgga gaggcggagt agccgaccgc cacgatgccc gagacgatgt
     2101 gccgttccgc gtggtagacg cagacgtacg cgacgccgta catgaacgcg ccctgcagcg
     2161 cgagcaggcc gtgctcgcgc agggcgaacc gcagccggtc gccacgccac gcggcgagcg
     2221 cgaggactac cgagcccgcg agcgcgaaac ggcacgcgac gccgacttcc ggaggcgtgg
     2281 cgccgagctg gaacgtgatg gcgtaccagg tcgtccccca cgtgaggacg caggcggcaa
     2341 acagctgcca ggacttgaag cggtgcatcg tgggcggagt cggacggcgg tcgcggccgc
     2401 cagtctgcca tgccatgctc gccggcgcgc cggccttgcg ttcggccagc cgttgcacga
     2461 tatttgaacg gtcgttgaaa gaatgctcgc gcacggatcg gcgcgtcctg cagggggaac
     2521 tggcttgttg catcgcacga agtcatcgcg accgttgctg gtcgcactgc tgatggcggc
     2581 cgaggcctgt ggccatgcgc cgacggactc gccggcgcgc cttggatcac tgccactcga
     2641 cccgcgggcc gtcacgctgt cgggcctgtc gtccggtggc gcgatggccg tgcagttcca
     2701 cacggcacac tcgcggctgg tgcagggtgt gggagtgttc gctgccccgc cgtactactg
     2761 cgcggaaggc gcgatcggca acgcgctcgg ccgctgcatg aaggacggca cctcgatccc
     2821 cgtcgagcgc ttgctgcagc aggccgaccg ctggtcgcag gaaggtctcg tcgacccggc
     2881 cgacggcgtt gcccaggaca aggtctggct gtatcgcggc gcggcggacc cgcacgtgca
     2941 cgtgaccgtg gccgacgcgc tcgaggggta ctaccgcgag cgcggcgtca gctccgccaa
     3001 cctcgtgcgg atcgagcgtg agggcgccgg ccacaacatg ccgatcgcgg cgcccggtgc
     3061 cgcaccgtgt gccgccggcg aaccaccgta
//
LOCUS       DASPFR010000095         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_152000, whole genome shotgun sequence.
ACCESSION   DASPFR010000095 DASPFR010000000
VERSION     DASPFR010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152000"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VD737_02410"
     CDS             <1..934
                     /locus_tag="VD737_02410"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HXW09439.1"
                     /translation="FAEGLNAPYGLALVGDRLYVANQDALVRFEYVDGQTQASGAPIK
                     VTDLPSELNHHWTKALAASADGRFLYVGIGSNSNITERGMTAEADRAVVWEVDAESGA
                     HRPYAEGLRNPTALAIQPDTGQLWAVVNERDELGPNLVPDYLTSVQEGRFYGWPYSYW
                     GNNVDTRVRPQDPERVASAIQPDYALGSHVAALGLAFSSPVMGAQYAEGVFIGEHGSW
                     NRGVPVGYKVVFVPFRNGRPAGEPIDFVTGFQSNGKTHGRPVGVAVDPRGALLVADDL
                     SNTVWRVTPTGAPAAVGQRDGMLEVPTEPAPPPP"
     gene            complement(961..1464)
                     /locus_tag="VD737_02415"
     CDS             complement(961..1464)
                     /locus_tag="VD737_02415"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HXW09440.1"
                     /translation="MRRILKSSSLVLLAVALAAPTAGLAQTVHATLSSHHEVPALSTP
                     GAGSFRAKVDVASGTIHWELQYANLEADTLQSHIHFGQAGVNGGVSAFLCTNLGNGPA
                     GTIACPARSGTLSGVITAATVIGPSGQGIAPGEFLALAAAILDGTAYVNVHTTAWPAG
                     EIRGQIE"
     gene            complement(1580..2323)
                     /locus_tag="VD737_02420"
     CDS             complement(1580..2323)
                     /locus_tag="VD737_02420"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09441.1"
                     /translation="MEALRPTVDEIADGTYRISTWVPDVTPVGFTFNQFLVTGDEPLL
                     FHTGPRGMFPLVAEAIAKVVPVESLRWITFGHVEADECGAMNEWLAIAPRAEVAHGAI
                     ACSVQLMDLADRAPRQLADAEVIDLGGGKRVRYLDTPHTPHGWDAGVLYEESTATLLC
                     GDLFTQVGNAAALTSNDVVGPALLAEDMFQFSALSPRMGSTIRRLAGLAPRTLALMHG
                     PSFNGDAAAALRALGDDYDRRVLEACQAR"
BASE COUNT          376 a          857 c          867 g          369 t
ORIGIN      
        1 cttcgccgaa ggtctcaacg ccccttacgg tctcgcactg gtcggtgatc ggctgtatgt
       61 cgccaaccag gacgcgctcg tgcgcttcga atacgtcgac ggccagacgc aggcctccgg
      121 cgcacccatc aaggtcacgg acctgccctc ggaactcaat caccactgga ccaaggcact
      181 ggccgcgagc gcggacggac gtttcctgta cgtggggatc ggctcgaaca gcaacatcac
      241 cgaacgcggc atgacggccg aggccgaccg cgcggtggtg tgggaagtcg acgcggaatc
      301 gggtgcgcac cgcccctatg ccgagggctt gcgcaatccc acggcgctcg ccatccagcc
      361 ggacacgggc cagctctggg cagtcgtcaa cgagcgcgac gaactcggac ccaacctcgt
      421 tccggactac ctgacgtcgg tgcaggaggg ccggttctat ggctggccgt acagttactg
      481 gggcaacaac gtcgacacgc gcgtccgacc gcaggacccc gagcgggtcg cctcggcgat
      541 ccagccagac tacgcgctgg gctcgcacgt cgccgcactg gggctggcat tctcctcgcc
      601 cgtgatgggc gctcaatacg cggagggcgt gttcatcggc gagcacggca gctggaatcg
      661 gggcgtgcca gtcggctaca aggtggtgtt cgtgcccttt cgcaacggcc ggccggccgg
      721 tgagccgatc gacttcgtga ccggcttcca gtcgaacggc aagacccacg gccggccggt
      781 aggcgtggcc gtcgatccac gcggcgcact gctcgtcgcc gacgacctct cgaacacggt
      841 gtggcgcgtc acgccgaccg gtgcccccgc ggccgttggg cagcgcgacg ggatgctgga
      901 ggttccgacc gagcctgctc ccccgccacc ctgacggggt cgaccgtcga gctcgccgcg
      961 ttactcgatc tggccgcgga tctcgcccgc cggccacgcc gtcgtgtgca cgttcacgta
     1021 ggccgtcccg tcgaggatcg ccgccgcgag cgccaggaac tccccgggtg cgatgccctg
     1081 cccgctcgga ccgatcaccg tcgcggccgt gatgacgccg ctcaacgtgc cgctgcgcgc
     1141 cgggcaggcg atcgttccgg ccggtccgtt gccgagattg gtgcagagaa acgcgctgac
     1201 cccgccgttg acgccggcct ggccgaaatg aatgtgcgac tggagcgtgt cggcctcgag
     1261 gttggcgtac tggagttccc agtggatcgt gcccgacgcg acgtcgacct tggcgcgaaa
     1321 actgccggcc cccggagtcg agagcgccgg tacctcgtga tggctgctca gggtggcgtg
     1381 cacggtctgg gccaggcccg ccgtcggtgc ggccagcgcg acggcaagca gtacgagcga
     1441 cgatgacttc aggatcctgc gcatcggagt ctcctcgggg tccacggtcg ggggcgtcgg
     1501 gcgtcacccg caggagagcg cggctcttcg accgggcaga cgccggaaac ccctgagtac
     1561 cttcaggcgc ctggacggtt catcgggcct ggcacgcctc gaggacacga cggtcgtagt
     1621 cgtcacccag cgcgcgcagt gccgcggctg cgtcaccgtt gaacgaggga ccgtgcatca
     1681 gcgccagcgt gcgcggcgcc aggccagcga gccgccggat cgtcgatccc atgcggggac
     1741 tcagtgcaga aaactggaac atgtcctccg cgaggagcgc gggcccgacg acgtcgttgg
     1801 acgtcagcgc cgcggcgttg ccgacctgcg tgaacaggtc gccgcacaac agcgtcgccg
     1861 tcgactcctc gtagagcacc ccggcgtccc agccgtgcgg cgtgtgtggc gtgtcgaggt
     1921 agcgcacgcg cttgccgccg ccgaggtcga tgacctccgc gtcggcgagt tggcgcggcg
     1981 cgcggtcggc gagatccatc agctgcaccg aacacgcgat tgcgccgtgc gcgacttcgg
     2041 cccgcggggc gatcgcgagc cactcgttca tggcgccgca ctcgtcggcc tcgacgtggc
     2101 cgaacgtgat ccagcgcagc gattcgaccg gcacgacctt ggcgatcgct tcggcgacca
     2161 gcgggaacat gccgcgtgga ccggtgtgga agagcagggg ctcgtccccg gtcaccagga
     2221 actggttgaa cgtgaagccc acaggcgtga cgtccggaac ccaggtggag atgcggtagg
     2281 tgccgtcggc gatctcgtcg accgtggggc gcagggcttc catgccccct cggcgttccg
     2341 tgcggcgccg gctcgttcaa cgcggacgga acgatcccgg tgtggccgtc cagcgcacct
     2401 cgccgtcgac gggcaggtag gcgcagtagg tgccggtgcg cacccggcgg tcgagcacgt
     2461 cgcccgcct
//
LOCUS       DASPFR010000096         6492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_30430, whole genome shotgun sequence.
ACCESSION   DASPFR010000096 DASPFR010000000
VERSION     DASPFR010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30430"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VD737_02425"
     CDS             complement(<1..382)
                     /locus_tag="VD737_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HXW09442.1"
                     /translation="MSVGALATNPAWLLPDWHEAPANLRALSTRRVGGVSVGPYASLN
                     LAAHVGDDPAAVEANRAILRNGASLPAEPLWLEQVHGTVVVEHLGQRPVVAPRADAAV
                     AFEPGRVCAVMTADCLPVVLGDRAG"
     gene            complement(379..1344)
                     /gene="rluD"
                     /locus_tag="VD737_02430"
     CDS             complement(379..1344)
                     /gene="rluD"
                     /locus_tag="VD737_02430"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: protein motif:HMM:NF008385.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HXW09443.1"
                     /translation="MTNSTVRVSLRIPDAQAGQRLDLALATLLPDYSRSRLKAWIESG
                     EVLVEGARRRPRDKVFGGEAVEVAASLPDEGTAQPQQIPLTIVHEDRHVLVLDKPAGL
                     VVHPGAGNPDSTLQNALLAHDAALAKLPRAGIVHRLDKDTSGLLIVARTLPAHTSLVR
                     MLEEREVHREYEAVCRGVMTGGGTVDAPIDRHPTDRVRMAIREGGRDAVTHYRVIRRF
                     RAHTHVRVQLETGRTHQIRVHLAHVGYPIVGDKVYGGRLALPKGGSEELRQALRAFPR
                     QALHAARLQLAHPVTGKPLEALAPLPADMTALLDRLATDAAAATA"
     gene            1404..2234
                     /locus_tag="VD737_02435"
     CDS             1404..2234
                     /locus_tag="VD737_02435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HXW09444.1"
                     /translation="MPSAPLKRAPMLTALLAAFAMTAGCGTFGWGGDDEEAFARDTPE
                     QVYRDAREDLQNGNYSNAVARYELLEARYPFSDQAKQGQLDLMYAYYKNRAGESAVDQ
                     AEQFIRENPTHPRVDYAHYIRGLVYFESGLTWIERVFKADLSQRPPTEARKSFQAFQT
                     LVQQFPKSPYAADARQRMVYLRNRLADYEVAVARFYLKRGAYVGAASRARGVIEAYDG
                     APAIDEALQVLAESYRKLGVDDLAQVAENVRKANALPDEFHKSAESKGDDDPWWRFWN
                     "
     gene            2424..5129
                     /locus_tag="VD737_02440"
     CDS             2424..5129
                     /locus_tag="VD737_02440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXW09445.1"
                     /translation="MKQLSGFLIVALFATLTVSAWGWANRPTPEPAWPRTIQGFAFQP
                     YQKDQDAIAGDEPTARQIAADLELVAGKARAVRTYSTLGTLASVPALARPHGIRVTIG
                     AWIDTDRVRNEREVEAAIRLANRHRNVTDIIVGNEVVLRGDLSAEDLQAHLDHVRAAT
                     KQPVSTAEPWHVWLEHPELANHVDFLAVHMLPYWEGVDAERAVEYVVDKLRRLEERFP
                     GRRIVIGEVGWPSDGRTRESAVATTANQALFLRRFLAYAEQHRLEYFVMEAFDQPWKE
                     AAEGQAGAYWGVYDADREQKFAFREAIVRVPQWQVLAAASVVTAAIMLWLFYFHSRSL
                     RNRGRSFLALVVYATATVVVWTLYDFSQQYLTVGSVLVGAVLIVGMLGVIAVLFAEAH
                     EWAEAHWVTKHRRLLRPRASQDARLPKVSIHVPAYNEPPELLCETLDALAALDYPDFE
                     VVVVDNNTVDESVWRPVEAHCARLGPRFRFFHVAPLAGFKAGALNFALARTSADAEIV
                     AVIDADYVVRPDWLRDLVPAFGNARTGVVQAPQDYRDAGENAFKAMCHAEYRGFFHIG
                     MVTRNERNAIIQHGTMTMVRRELLERIGWAEWCITEDAELGLRIFDEGYEATYVAQSY
                     GRGVMPDTFLDFKKQRARWAFGAMQIMRAHAAALFRGRGTGLTAGQRYHFIAGWLPWL
                     ADGFNLVVNCAALAWSLVMVAFPRHVEPPLLMFSVLPLSLFVFKLVKLLHLYRTRVGA
                     NLRQTLAAALAGLSLSHTIGSAMLGGLVRRDRPFFRTPKRAARHAWLQGLAAAREEAF
                     LMAGLWFAAFAVSHIPEVDGSLTGLVGSPDLTVWVAVLLIQSVPYAAAVVVSVVSGLS
                     LPGAWIGEAGAAAVVPVGPSTMQEPAPAVNNGGPVPVTAKLDSAK"
     gene            5126..>6492
                     /locus_tag="VD737_02445"
     CDS             5126..>6492
                     /locus_tag="VD737_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HXW09446.1"
                     /translation="MNAIPDPLPAPATTAPAAAPDLDPPPRLREIPYNYTSFADREIV
                     IRLLGAEGWALLDDLRAERRTGRSARMLYEVLGDIWVVRRNPYLEQDLLDNPKRREQL
                     VEALHHRLADIEKRRDASDPARDAKVGRLLEFARRAIAAFAESFAHVEALRRRARRAF
                     AAHTRGDNVCFDAYARVAHATDATDWRVELPFVVLTPDAEDEVPGLVRACIDSGLTII
                     PRGGGTGYTGSAVPLTPYAAVINTEKLERIDAVERRTLPGLGEPVPTLYTEAGAVTRR
                     VEEAAHAAGFVFAVDPTSADASCVGGNIAMNAGGKKAVLWGTALDNLAWWRMVGPDGH
                     WLEVTRIGHNRGRIHDVATATFELTWKDGSQPAASARVLRIERLELPGRLFRKTGLGK
                     DVTDKFLGGLPGVQKEGCDGIITSARFVLHRMPSSIRTVCLEFFGPARVSTPSIVEIK
                     RYLDTR"
BASE COUNT          961 a         2323 c         2249 g          959 t
ORIGIN      
        1 tgcccgcgcg atcgccgagc acgaccggca ggcaatccgc cgtcatgacc gcgcagacgc
       61 gtccgggctc gaacgcgacc gcggcgtccg cccgcggcgc cacgaccggt cgctggccaa
      121 ggtgctcgac gacgacggta ccgtgcacct gttcgagcca cagcggttcc gcgggcaacg
      181 aggcgccgtt gcgcaggatc gcgcgattgg cttcgaccgc ggccggatcg tcaccgacgt
      241 gcgcggcgag gttgagactc gcgtacggcc cgacgcttac gccgccgacg cggcgcgtcg
      301 agagcgcccg caggttcgcc ggcgcctcgt gccagtcggg cagcaaccag gccgggttgg
      361 tcgcgagcgc gccgacgctc atgcggtcgc cgccgccgcg tccgtcgcca gccggtcgag
      421 cagtgcggtc atgtccgccg gcaacggcgc caacgcctcg agcggcttgc ccgtgacggg
      481 gtgcgccagc tggagccgcg ccgcgtgcag cgcttggcgc gggaaggcgc gcaacgcctg
      541 ccgcagttcc tcgctgccgc ccttgggcag cgccagtcgc ccgccgtaga ccttgtcgcc
      601 gacgatcgga tacccgacgt gcgcgaggtg cacgcggatc tggtgggtgc gaccggtctc
      661 gagctggaca cgcacgtgcg tgtgtgcgcg gaagcgccgg atcacgcggt aatgcgtcac
      721 ggcgtcgcgg ccgccctcgc ggatggccat gcgcacgcga tcggtgggat gccgatcgat
      781 cggcgcgtcg acggtgccgc cgcccgtcat cacgccgcga cagacggcct cgtattcgcg
      841 atggacctcc cgctcctcga gcatgcgcac cagggacgta tgcgccggca gcgtgcgcgc
      901 gacgatcagc agcccgctcg tgtccttgtc caggcggtgc acgattcccg cacgcggcag
      961 ctttgcgagc gcggcgtcgt gcgcgagcag cgcgttctgc agcgtgctgt ccgggttgcc
     1021 ggccccgggg tgaacgacga ggccggcagg cttgtccaag accaggacat gcctgtcctc
     1081 gtgcacgatc gtgagcggaa tctgctgggg ctgcgcggtg ccctcgtcgg gcagcgaagc
     1141 ggcgacctcg acggcctcgc cgccgaacac tttgtcgcgc ggacgacgcc tggcgccttc
     1201 gaccagcact tcgcccgact cgatccacgc cttgagccgg ctgcgcgagt agtcgggaag
     1261 cagcgtggcg agcgcgagat cgagccgctg gccggcctgc gcgtcgggga ttcgcaggct
     1321 cacgcggacc gtggaattcg tcatggaggc gaattctata tacttcgcgt ttgcccgcag
     1381 gccccgctcc aggacctccc cgcatgcctt ccgccccgtt gaaacgcgcc ccgatgctga
     1441 ccgcgctgct ggcggccttc gccatgacgg cgggctgcgg cacgttcggc tggggcggtg
     1501 acgacgagga ggcctttgcc agggataccc cggagcaggt ctatcgcgat gcgcgcgagg
     1561 atctccagaa cggcaactac tcgaacgccg tcgcacgcta tgaactgctc gaggcgcgat
     1621 atccgttcag cgaccaggcc aagcagggcc agctcgacct gatgtacgcg tactacaaga
     1681 atcgagccgg cgagtccgcg gtggaccagg cggagcagtt catccgcgag aacccgacgc
     1741 atccgcgcgt cgactacgcg cactacatcc gcggcctcgt ctacttcgag tccggcctga
     1801 cctggatcga gcgcgtgttc aaggccgacc tctcgcagcg accgccgacc gaagcccgca
     1861 agtcgttcca ggcattccag acgctggtcc agcagtttcc gaagagcccg tacgccgcgg
     1921 acgcccgcca gcggatggtc tacctgcgca accggctggc ggactacgaa gtggcagtcg
     1981 cacgcttcta cctgaagcgc ggcgcctacg tcggtgccgc gagccgtgcg cgcggcgtca
     2041 tcgaggcgta tgacggcgcg ccggcgatcg atgaggcgct ccaggtgctc gcggagtcgt
     2101 atcggaagct gggcgtcgac gacctcgccc aggtcgccga gaacgtgcgc aaggccaacg
     2161 cgctgccgga cgaatttcac aaatcggccg agtccaaagg ggacgacgac ccttggtggc
     2221 gtttctggaa ctgaacgttc cacggcgatc ggccgcggcc cgaacggttc gcgcgcacta
     2281 accacctgat tcagcgagaa aatgcaccga agcggtgcag ggccggtcgt gtccgcgccg
     2341 atgtcgccaa ttcccgacgc ttgtggcccc cgggttttgc tgcaccgcaa ctaaaatccc
     2401 gcgctctcct gcgcgggtgg acgatgaagc agctgtccgg attcctgatc gtggccctgt
     2461 ttgcgacgct gaccgtgtcg gcctggggct gggccaaccg gccgacgccg gaaccggcct
     2521 ggccgcgcac catccagggc tttgcgttcc agccgtacca gaaggaccag gacgcgatcg
     2581 ccggcgacga gccgacggct cgccagatcg ccgccgacct cgagctcgtc gcgggcaagg
     2641 cgcgcgccgt gcgcacctac agcacgctcg gcacgctggc gagcgtcccc gcgctggcgc
     2701 gaccgcatgg catccgcgtc acgatcggcg cgtggatcga caccgaccgg gtgcgcaacg
     2761 aacgcgaggt cgaggccgcg atccgcctcg cgaaccggca ccgcaacgtc acggacatca
     2821 tcgtcggcaa cgaagtcgtg ctgcgcggcg acctctcggc cgaggacctg caggcgcacc
     2881 tcgaccacgt gcgcgcggcg acgaagcagc cggtcagcac ggccgagccg tggcacgtct
     2941 ggctggagca tccggagctc gcgaaccacg tcgacttcct ggcggtgcac atgctgccgt
     3001 actgggaagg cgtcgacgcg gaacgggccg tcgaatacgt cgtcgacaag ctgcggcggc
     3061 tcgaggagcg tttcccaggc cgccgcatcg tgatcggcga ggtcggctgg cccagcgacg
     3121 ggcgcacgcg cgagtccgcg gtggcgacga ccgcgaacca ggcgctcttc ctgcgccggt
     3181 tccttgccta cgccgagcag caccggctcg agtacttcgt catggaggcg ttcgaccagc
     3241 cctggaagga agccgccgaa ggccaggccg gcgcctactg gggcgtctac gacgccgacc
     3301 gggaacagaa gttcgcgttc cgtgaagcga tcgtccgcgt gccgcagtgg caggtgctcg
     3361 cggccgcctc ggtggtcacc gccgcgatca tgctgtggct gttctatttc cacagccgct
     3421 cgttgcgcaa ccgcggacgc agcttcctcg cgctggtcgt atacgcgacg gcgaccgtgg
     3481 tcgtctggac gctctacgac ttctcgcagc agtacctgac cgtcggcagc gtgctcgtgg
     3541 gcgccgtgct gatcgtgggc atgctcggcg tgatcgccgt gctgttcgcc gaagcgcacg
     3601 agtgggccga ggcgcactgg gtcacgaagc accggcgcct gctgcggccg cgcgcctcgc
     3661 aggacgcgcg gctgccgaag gtgtcgatcc acgtgcccgc ctacaacgag ccgccggaac
     3721 tgctctgcga gacgctcgac gcactcgccg cgctggacta cccggacttc gaggtcgtcg
     3781 tcgtcgacaa caacacggtc gacgagtccg tgtggcgacc ggtcgaggcg cactgcgcgc
     3841 ggctcggccc gcgcttccgc ttcttccacg tggcgccgct cgcgggcttc aaggccgggg
     3901 cgctcaactt tgccctcgca cgcacgtcgg ccgacgcgga gatcgtcgcc gtgatcgacg
     3961 cggactacgt cgtgcggccc gactggctgc gcgacctcgt gccggcgttc ggcaatgcgc
     4021 gcacgggcgt ggtgcaggcg ccgcaggact atcgcgacgc gggcgagaac gcattcaagg
     4081 ccatgtgcca cgccgagtat cgcggcttct tccacatcgg catggtcacg cgcaacgagc
     4141 gcaacgcgat catccagcac ggcacgatga ccatggtgcg ccgcgaactg ctcgaacgga
     4201 tcggctgggc ggagtggtgc atcaccgagg acgccgagct cgggctgcgc atcttcgacg
     4261 agggctacga ggcgacctac gtcgcccaga gctacggccg tggagtgatg cccgacacct
     4321 tcctcgactt caagaagcag cgcgcgcgct gggcgttcgg cgcgatgcag atcatgcgcg
     4381 cacacgcggc tgcgctgttc cgcggccgcg gtacgggcct cacggccggc cagcgctacc
     4441 acttcatcgc cggttggctg ccctggctcg ccgacggatt caacctcgtc gtgaactgcg
     4501 cggcgctcgc ctggtcgctg gtgatggtcg cgttcccgcg ccacgtcgag ccgccgctgc
     4561 tgatgttctc ggtgctgccg ctgtcgctgt tcgtgttcaa gctcgtcaag ctgctgcacc
     4621 tgtaccgcac gcgtgtcggc gcgaacctgc gacagacgct cgccgcggcg ctcgcgggcc
     4681 tgagcctctc gcacacgatc ggctcggcga tgctgggcgg actcgtgcgc cgcgaccgtc
     4741 cgttcttccg cacgcccaag cgcgcggcac gccacgcctg gctgcagggg ctcgcagcgg
     4801 cgcgcgaaga ggcgttcctg atggcggggc tgtggttcgc cgcgttcgcg gtgtcccaca
     4861 tcccggaggt cgacggcagc ctgacgggcc tcgtcggcag ccccgacctc acggtctggg
     4921 tcgcagtgct gctgatccag tcggtcccgt acgccgccgc cgtcgtcgtg tccgtcgtca
     4981 gcggcctgtc actgccgggc gcctggatcg gcgaggcggg cgccgcggcg gtcgtgccgg
     5041 tcggaccttc cacgatgcag gagccggccc cggcggttaa caatggcggg cccgtccccg
     5101 tcaccgccaa gctcgactcc gcgaagtgaa cgccatcccg gaccccctcc ccgcgccagc
     5161 cacgaccgcc cccgccgccg cgccggacct cgacccgccg ccgcggctgc gcgagattcc
     5221 ctacaactac acgtcgttcg ccgatcgcga gatcgtgatc cggctgctcg gcgccgaagg
     5281 ctgggcgctg ctcgacgacc tgcgtgccga gcggcgcacc gggcgctcgg cgcggatgct
     5341 gtacgaggtg ctcggcgaca tctgggtggt gcgccgcaac ccgtacctgg agcaggacct
     5401 gctcgacaac ccgaagcgcc gcgaacaact ggtcgaggcg ctgcatcacc ggctggccga
     5461 catcgagaag cgtcgcgacg cgagcgatcc cgcgcgcgat gccaaggtcg gtcgcctgct
     5521 cgaatttgca cgccgcgcca tcgcggcgtt cgccgagtcg ttcgcgcacg tcgaggcgct
     5581 gcggcgccgg gcgcgtcgtg ccttcgcggc gcacacgcgc ggcgacaacg tctgcttcga
     5641 cgcgtacgcg cgcgtcgcgc acgcgaccga cgcgaccgac tggcgcgtcg aactgccgtt
     5701 cgtcgtgctg acacccgatg ccgaggacga agtgcccgga ctggtgcgcg cctgcatcga
     5761 cagcggcctc acgatcatcc cgcgcggcgg cggcacgggc tacaccggca gcgcggtgcc
     5821 gctcacgccc tatgcggccg tcatcaacac cgagaagctc gagcgcatcg acgcggtcga
     5881 gcgccgcacg ctgcccggcc tgggcgagcc cgtgccgacg ctctacacgg aggccggcgc
     5941 cgtcacgcgc cgggtcgagg aagcggcaca cgcggcggga ttcgtgttcg ccgtggaccc
     6001 gacgtccgcg gacgcctcgt gcgtcggcgg caacattgcc atgaacgccg ggggcaagaa
     6061 ggcggtgctg tggggcaccg cgctcgacaa cctcgcgtgg tggcgcatgg tcggccccga
     6121 cggccactgg ctggaggtca cgcggatcgg ccacaaccgc ggcaggatcc acgacgtcgc
     6181 gaccgcgacg ttcgaactca cctggaagga cggcagccag cccgccgcga gtgcgcgcgt
     6241 gctgcgcatc gagcggctcg agctgcccgg gcggctgttc cgcaagacgg gcctcggcaa
     6301 ggacgtcacc gacaagttcc tcggcggcct gcccggcgtg cagaaggaag gctgcgacgg
     6361 catcatcacc agcgcgcgct tcgtgctgca ccggatgccg tcgtcgatcc ggaccgtttg
     6421 cctcgagttc ttcggcccgg cacgcgtgtc gacgccatcg atcgtcgaga tcaagcgcta
     6481 cctcgacacg cg
//
LOCUS       DASPFR010000097         3522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1738940, whole genome shotgun sequence.
ACCESSION   DASPFR010000097 DASPFR010000000
VERSION     DASPFR010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738940"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VD737_02450"
     CDS             <1..631
                     /locus_tag="VD737_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXW09447.1"
                     /translation="TRRVFVHESRLDELERRLVSAYKQVRIGDPLTQGTLMGPLIDAS
                     AVERYEAAVAQAVKNGGRLLCGGKRLDRPGYFVEPAIVVAKGEWEIVRTETFGPVLYL
                     VPFRTLDEAIAAQNEVAHGLSSAIFTDRLQHAERFLSVAGSDCGIANVNIGTSGAEIG
                     GAFGGEKDTGGGRESGSDSWKAYMRRQTCTVNWGTELPLAQGINFDVGA"
     gene            complement(695..1585)
                     /locus_tag="VD737_02455"
     CDS             complement(695..1585)
                     /locus_tag="VD737_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXW09448.1"
                     /translation="MLAAAAMAQQPSIVAAKPAPLQVQQVREGFSVVSGAGGNVVVWA
                     GTDGVVLIDTGLASRAPELFETVARIAPGALRFVINTHAHVDHTGGNEAAGTRGAVIV
                     AHDRLLERTGLDASDASAGASQGSDSVAPAATLPVVTTSDRVSLHVNGDRLDVVHVAD
                     AHTSADLVVRWTDANVVALGDVFWNGQYPYVDVAAGGSLVGLVAAVEGSLARVNAQTT
                     IVPGHGAVANREQLSAYRDMLVAVGRKVREAVERGEGLQQLQASHPTAEFDARYFRAG
                     ASITPDDFVRNLYEDVARRR"
     gene            1725..3131
                     /locus_tag="VD737_02460"
     CDS             1725..3131
                     /locus_tag="VD737_02460"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HXW09449.1"
                     /translation="MTSLVRPGIVRRRATAGWVALLLGLLTLGALRAAPETQSEGVLI
                     PVTGAIGPGTSDFFVRQLTAAQEAGAHLVVLRIDTPGGLETSMRDMIQAILGSSVPVI
                     GYVAPSGARAASAGTYLMYASHVAAMAPATNLGAATPVQIGGSPPGGDSKPAQPEPKD
                     EKNGDATESPALPGSASERKAVNDSVAYIRGLAELRGRNVDWAERAVREGVSLPAIEA
                     LEANVIDLVASDLPELLKALDGRVVKVASGDVTLQTEGIVLREVQPDWRTELLQLITN
                     PNVAYLLMLIGIYGLLLEGYNPGAVVPGVVGAISLVLALFAFQVLSVNYAGLALIALG
                     VLLIVAESFVPSFGTLGLGGIVAFVIGSVMLLDNEVPGFGIAWQMIAGMAFAASLLML
                     AIVTFAVRSRRRPVVTGQAGLLHECAEAVEAFDRRGLVRVQGVLWNAVTQVPVQAGQR
                     LRIVRVDGLTLQVEPAAD"
     gene            3154..>3522
                     /locus_tag="VD737_02465"
     CDS             3154..>3522
                     /locus_tag="VD737_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXW09450.1"
                     /translation="MFSIPTLGIVVVIAIVLATQIFKILNEYERGVVFTFGRYTGIKG
                     PGIIVLIPFVQRMVKVDLRVIVLDVPTQDVISRDNVSVKVNAVIYFQIVQPDKAIIQV
                     ASPFEATSQLAQTTLRSVLGQ"
BASE COUNT          545 a         1211 c         1226 g          540 t
ORIGIN      
        1 gactcgacgc gtattcgtgc acgagtcgcg actcgacgag ttggaacgcc gcctggtcag
       61 cgcttacaag caggtccgca tcggcgaccc gctgactcag ggcacgctga tgggtcccct
      121 gatcgacgcg agcgccgtcg agcgctacga ggcggcagtc gcacaggccg taaagaacgg
      181 cggtcggctg ctgtgtggcg gcaagcggct cgatcggccg gggtacttcg tcgagccggc
      241 gatcgtcgtc gcgaagggcg agtgggaaat cgtgcggacc gagacgttcg gcccggtgct
      301 ttacctggtg ccgttcagga ccctcgacga ggcgatcgcg gcgcagaacg aggtcgcgca
      361 cgggctctcg tccgcgatct tcaccgatcg cctgcagcac gccgagcgct ttctgtcggt
      421 ggccggctct gactgtggga tcgccaacgt caacatcggc acttcgggcg ccgagatcgg
      481 cggcgcgttc ggaggcgaga aggacactgg cggtggacgc gagtcaggtt ccgactcgtg
      541 gaaggcttac atgcgtcgcc agacctgcac ggtcaactgg ggcaccgagc tgccgctcgc
      601 gcaggggatc aacttcgacg tcggggcgtg acggcgcacc gaaaatcagg agtgtcccca
      661 tttagcgcgc accgaaaatg gggactgtcc ccaattaccg gcggcgcgcg acgtcctcgt
      721 acaggttgcg gacgaaatcg tcgggcgtga tcgaagcgcc ggcgcgaaag taccgcgcat
      781 cgaattccgc ggtcggatgc gaggcctgaa gttgctgcag gccctcgccc cgctcgacgg
      841 cctcacgcac cttgcgaccc acggcgacca gcatgtcgcg ataggcggac agctgctcgc
      901 ggttcgccac ggcgccgtgg ccgggcacga tcgtggtctg cgcgttcacg cgcgccagcg
      961 aaccctcgac cgctgccacc agcccgacga gcgagccccc ggcggccacg tcgacgtaag
     1021 gatactggcc gttccagaag acgtcgccca gcgcgaccac gttggcgtcc gtccagcgca
     1081 cgacgaggtc ggccgacgtg tgggcgtccg ccacgtgcac gacgtcgagc cggtcgccat
     1141 tcacgtgcaa cgagacgcgg tccgacgtcg tcacgaccgg gagggtcgcg gcgggcgcaa
     1201 cggagtcgct gccctgggac gcaccggcgg aggcatcgga ggcgtcgagc ccggtgcgtt
     1261 cgagcagccg gtcgtgtgcc acgatcaccg cgccgcgagt gcccgcggcc tcgttgccgc
     1321 ccgtgtggtc gacgtgcgcg tgcgtgttga tcacgaaacg cagcgcgcca ggcgcgatgc
     1381 gcgcgaccgt ctcgaacagt tccggcgcgc gactcgccag tccggtgtcg atgagcacca
     1441 cgccgtccgt cccggcccag acgacgacgt tgccgcccgc gccagagacc acgctgaatc
     1501 cttcccgcac ctgctgcacc tgcagcgggg ccggcttggc cgcgacgatg gacggctgct
     1561 gcgccatcgc cgccgcggcc agcaacgcca ctccgcaccc gccgacgatg gctgacaccg
     1621 acgaccggtg gacgcgagac gcacggacgg ggagctgcgg ctgtagcacg ctcgaaagta
     1681 taggccagcc acggccgcgt cgacggctgc ggtatcatgc cgtcatgacc tcgctcgtcc
     1741 gacccgggat cgtgcgtcga cgcgccacgg caggatgggt cgcgctgctg ctgggcctgc
     1801 tcacgctagg cgcactgcgc gcggcccccg agacgcagtc cgaaggcgtg ctgatccccg
     1861 tgaccggcgc catcggtccg ggcacgagtg acttcttcgt gcgccagctc accgctgcac
     1921 aggaggccgg cgcgcacctc gtcgtgctgc ggatcgacac gcccgggggc ctcgaaacct
     1981 cgatgcgcga catgatccag gccatcctcg ggtccagcgt ccccgtgatc ggctacgtcg
     2041 cgccgagcgg cgcgcgggcc gcgagtgcgg gcacctacct gatgtacgcg agccacgtcg
     2101 ccgccatggc gcctgccacc aacctgggcg ccgccacgcc ggtgcagatc ggcggttcgc
     2161 ccccgggcgg cgactcgaag cctgcccaac ccgagccgaa ggacgagaag aatggcgatg
     2221 cgaccgagtc gcccgcgctg ccgggatcgg cttccgaacg caaggcggtc aacgactcgg
     2281 tggcgtacat ccgcgggctc gccgaactgc gggggcgcaa cgtcgactgg gccgagcgtg
     2341 ccgtgcgcga aggcgtgagc ctgcccgcga tcgaagccct cgaggcgaat gtgatcgacc
     2401 tggtcgcgag cgacctcccc gagctcctga aggcgctcga cggacgcgtc gtcaaggtcg
     2461 cgagcggcga cgtcacgctg cagaccgaag gcatcgtgct gcgcgaggtg cagccggact
     2521 ggcgcaccga gctgctgcag ctgatcacga acccgaacgt cgcctacctg ctgatgctga
     2581 tcggcatcta cgggttgctg ctcgagggct acaacccggg cgcggtggtg cccggcgtgg
     2641 tcggtgcgat cagcctggtg ctggcgctgt tcgcgttcca ggtgctgtcc gtcaactacg
     2701 ccgggctcgc gttgatcgcg ctcggcgtgc tgctgatcgt cgccgaatcg ttcgtgccct
     2761 cgttcggcac gctcggcctg ggcggcatcg tcgctttcgt gatcggctcc gtgatgctgc
     2821 tggacaacga agtccccggc ttcggcatcg cctggcagat gatcgcgggg atggcgttcg
     2881 ccgccagcct gctcatgctc gccatcgtca ccttcgccgt gcgctcgcgc cggcggccgg
     2941 tcgtgaccgg gcaggcaggc ctgctgcacg agtgcgccga ggccgtcgag gcgttcgacc
     3001 gccgcgggct ggtgcgcgtg cagggcgtgc tctggaacgc cgtgacgcag gtgccggtac
     3061 aggcgggcca gcggctgcgc atcgtgcgcg tcgacggcct cacgctgcag gtcgagcccg
     3121 ccgccgacta gatcatccca ggagattcaa tcgatgttca gcatcccgac cctaggcatc
     3181 gtcgtcgtca tcgcgatcgt gctggcgacg cagatcttca agatcctcaa cgagtacgag
     3241 cggggcgtcg tgttcacgtt cgggcggtac accggcatca aggggccggg catcatcgtg
     3301 ctgatcccgt tcgtgcagcg catggtcaag gtggacctgc gcgtgatcgt gctggacgtg
     3361 ccgacgcagg acgtgatctc ccgcgacaac gtttccgtca aggtcaacgc ggtcatctat
     3421 ttccagatcg tccagcccga caaggcgatc atccaggtgg cgagcccctt cgaggcgacc
     3481 agccagctcg cgcagacgac gctgcgctcg gtgctcggcc ag
//
LOCUS       DASPFR010000098         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_396537, whole genome shotgun sequence.
ACCESSION   DASPFR010000098 DASPFR010000000
VERSION     DASPFR010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396537"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="VD737_02470"
     CDS             <1..1014
                     /locus_tag="VD737_02470"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HXW09451.1"
                     /translation="IPDSPVSPIWNPEFFGNMMVVNGFTWPYLEVEPRRYRLRFLNGC
                     NSRFLILDFTSIPGVEVWQIGNEGGFLAAPVNLTEGNANRVLLGLAERADVIVDFTRV
                     PAGNYVLRNVGPDEPFGGGTPGVDFPLPDPETTGQVLEFSVVPALATDPTTPPRYLQL
                     PPITPLPVATVTRRLALIERMGMGFNSQGEPIEGPVEALLGRVVAGRLVADAWMDPLT
                     ENPTPGATELWELYNATGDAHPMHIHDVVFEVVDRQSIVLDAEGEVVEPVQLADDPVP
                     PAPWETGFKDTVIAYPGQVTRVRAQFKSPGQFVWHCHIVEHEDNEMMRPFRVGPAQPG
                     QPA"
     gene            complement(1044..1130)
                     /locus_tag="VD737_02475"
     tRNA            complement(1044..1130)
                     /locus_tag="VD737_02475"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1094..1096),aa:Leu,seq:taa)
     gene            1283..>2238
                     /locus_tag="VD737_02480"
     CDS             1283..>2238
                     /locus_tag="VD737_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXW09452.1"
                     /translation="MHPCEGSTLRATSVLARLTSVLVACLCCTGIARSATTSDGTVST
                     LATATRPNVVIVLADDLDVHSLTRMMNLGLMPKLKTNVVNPGTQFRNSFVTTSWCCPS
                     RATLLTGLYSHNHRVLTNSRPLGGVHRFDDSSTLATWLQKSGYRTGLVGKYFNNYGSD
                     NDATTPVDDPRYVPPGWTDWQGLQDKAVDGRRAFQMYDYTINDNGTLVRHGTAPGDYQ
                     TDVLARRASQFVEDAESANDAQPFFLLVAPTAPHLEQPKPVTGGCSDTEWANSIRPAP
                     RHVGTLPATVQVPRPGNFNEADLRDKPSWFQRVPALTTLDVG"
BASE COUNT          388 a          771 c          710 g          369 t
ORIGIN      
        1 atcccggaca gcccggtctc gccgatctgg aacccggaat tcttcgggaa catgatggtg
       61 gtcaacggct tcacgtggcc gtacctcgag gtcgagccgc gccgctaccg cctccgcttc
      121 ctgaacggct gcaactcgcg cttcctgatc ctcgacttca ccagcatccc cggcgtcgag
      181 gtctggcaga tcggcaacga gggaggtttt ctcgcggcgc cggtcaacct gaccgaaggc
      241 aatgcgaatc gcgtgttgct cgggcttgcc gagcgcgcgg acgtgatcgt ggacttcacg
      301 cgcgtacccg ccggcaatta cgtcctgcgc aatgtggggc cggacgagcc tttcggcggc
      361 ggcacgcccg gtgtggattt tccgttgcct gatcccgaga cgacgggaca ggtactcgag
      421 ttcagcgtgg tgcccgcgct cgcgaccgat ccgacgaccc cgccccgtta cctacagctt
      481 ccaccaatca cgccattgcc cgttgccacc gtgacacgcc gcctggcgct gatcgaaagg
      541 atgggcatgg gcttcaattc acagggcgag ccgatcgaag gtcccgtgga agccctgctc
      601 gggcgagtgg tggccggccg cctggtcgcc gacgcctgga tggacccgct tacggagaac
      661 ccgaccccgg gcgccacgga gctctgggaa ctatacaacg cgacaggcga tgcgcacccg
      721 atgcacattc acgacgtggt cttcgaagtc gtggatcgcc agtcgatcgt cctggacgcc
      781 gagggcgaag tcgtcgagcc cgtgcaactg gcggatgacc cggtgccacc ggccccttgg
      841 gaaacgggtt tcaaggacac cgtaattgcg taccccggcc aggtgacgcg cgtgcgcgcc
      901 cagttcaagt cacccgggca attcgtctgg cactgccaca tcgtcgagca cgaagacaac
      961 gagatgatgc ggcctttccg ggtggggccc gcgcagccgg ggcagccggc ctgagggcca
     1021 ccgcacggtg cgcgaacacc ggttggtgcc ccgggccgga ctcgaaccgg cacgcccttg
     1081 cgggcaacgg attttaagtc cgtagcgtct accggttccg ccaccggggc cgaccgcggg
     1141 caaattatgc ccgttcgcga ccaaaacagg ccgttcgctt cgccggcgta ggtcgacttg
     1201 gcgaacgtga acgtagtacg gtctcggctg gcgtatccca cttccggata cgtcgcccat
     1261 cgacgacaag gagcgttgta cggtgcatcc atgcgaaggc tcgaccctgc gcgcgacgag
     1321 cgtgctcgcc cgactgacct ccgtgctcgt cgcctgcctg tgctgcaccg gcatcgcccg
     1381 cagcgccacg acctccgacg gcacggtgtc gacactggca acggccacgc gccccaacgt
     1441 cgtgatcgtg ctcgccgacg acctcgacgt ccattccctc acgcgcatga tgaatcttgg
     1501 gctgatgccc aagctcaaga cgaacgtcgt caatcctggc acgcagtttc gcaactcgtt
     1561 cgtcactacc tcgtggtgct gtccctcgcg ggcgacgctg ctgaccggcc tgtattcgca
     1621 caatcatcgc gtgctcacca acagccggcc gctgggtggc gtgcatcgct tcgatgattc
     1681 gtcgacgctc gcgacgtggc tgcagaagtc tggttaccga acggggctcg tcggcaagta
     1741 cttcaacaac tacggcagcg acaacgacgc gacgacgcct gtcgacgacc cacgatacgt
     1801 gccgccgggc tggaccgatt ggcagggcct tcaggacaag gccgtggacg gacgtcgcgc
     1861 gttccagatg tacgactaca cgatcaacga caatggcacg ctggtgcggc acggcaccgc
     1921 gccgggcgat taccagaccg acgtgctcgc gcggcgggcg agtcagttcg tcgaagacgc
     1981 cgagtccgcg aacgacgcgc agccgttctt cctgctggtc gccccgacgg ctccgcacct
     2041 cgagcagcct aagccggtca ctggcggatg cagcgacacg gagtgggcca actcgatacg
     2101 cccggcgccg cgacacgtcg gcacgctgcc ggcgaccgtc caggtgccga ggcccgggaa
     2161 tttcaacgag gcggacctgc gcgacaagcc cagctggttc cagcgcgtgc ccgcactgac
     2221 gaccctcgac gtcggctg
//
LOCUS       DASPFR010000099         6002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_640044, whole genome shotgun sequence.
ACCESSION   DASPFR010000099 DASPFR010000000
VERSION     DASPFR010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640044"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VD737_02485"
     CDS             <1..703
                     /locus_tag="VD737_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXW09453.1"
                     /translation="DLDVAASRIAWGKFINAGQTCVAPDHVLVHREVAAQFADTLAAK
                     IKQFYGDDPAQSPDFCRIASERHTARFAKLLEGQKIHSGGQVDVARRYVAPTIVLDPD
                     PESELMREEIFGPVLPVITVDEMHHAIKFVADRPKPLALYLFSKSKALEDAVLSRLSA
                     GSVCINDAVIFMVSPELPFGGIGASGMGRYTGWYGFETFSHMKPVMKRSFRFDAPLRY
                     PPYDDFKAKVMRLVR"
     gene            712..1410
                     /locus_tag="VD737_02490"
     CDS             712..1410
                     /locus_tag="VD737_02490"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HXW09454.1"
                     /translation="MDPLDPDLLDMALRILVSLAAGALIGYERSYRGRPAGFRTHALV
                     CMASTLLMLLTVYESHWVETPDALVRLDPTRMAQGIMTGIGFLGAGVIMREGLSVRGL
                     TTAASIWITAAIGVLAGAGFYFPLVLAVALTLGVLSLFRLIEVRMPTQAYYHFDVRFA
                     PDATHGEQTVRALVAKHGFTVANFSYRLDDEGRVRRYTTTIASTDRSAAGRLAITLDQ
                     HGDVHEYRIAPTSD"
     gene            complement(1424..1852)
                     /locus_tag="VD737_02495"
     CDS             complement(1424..1852)
                     /locus_tag="VD737_02495"
                     /inference="COORDINATES: protein motif:HMM:NF039479.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6524 family protein"
                     /protein_id="HXW09455.1"
                     /translation="MASTDGTDFRVTGVLVRVAFSLALVFLTFNPTGLSYYHWLSSHL
                     SPIDPLVVVAGIALLGAWLFFVRATFSSMGAVGVVLLLALFASIVWWIVSKGWLSVEN
                     RSAMSWVVLTCLGLLLGIGMSWAHLRARISGQASVDRVDA"
     gene            1949..4042
                     /locus_tag="VD737_02500"
     CDS             1949..4042
                     /locus_tag="VD737_02500"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HXW09456.1"
                     /translation="MNAIAIPAPHATLNPAQAEAVTHGQPRSGGGVAAGPLLVIAGAG
                     TGKTNTLAHRVAHLLLQGVAPERILLLTFTRRAALEMTRRAQRVMAQALAARGPGHAT
                     VPENLRLSWSGTFHAIGNRLIREYAPRLGLDPGFTVLDRGDAADLFDVVRHRLGLSKT
                     EKRFPRKDICLAIYSHRINTQRPLRHTLETVYPWCLDWEQELTSLCRAYVEAKLEQQV
                     LDYDDLLLYWHVMTRDPAIAGELGARFDHVLVDEYQDTNVLQAEILLHLKPDGAGLTV
                     VGDDAQAIYSFRAASVDNILEFPQKFTPPARVVTLEQNYRSTQGVLDSANALMDEAGR
                     QYSKQLRSARGPGARPQYVTVLDEQAQATFVIDRVLEARERGVDLRRQAVLFRNSDHS
                     DVLELELVRRNVPYVKYGGLKFLEAAHVKDLLAVLRWADNPRNRIAAFRVLQLLPGMG
                     PANAQRACEAFEAASFAWTALAAHAVPAAARDGWAALVALLVQIGADGAPWPGQVERV
                     RRWYEPQLERIYESAQARKGDLDQLDRLSQQHASRESFVTELTLDPPQATGDLSGDPL
                     LDEDYLVLSTVHSAKGQEWDTVHVLNVTDGSFPSEFASGRTELLEEERRLLYVAMTRA
                     KDELRVVAPVRFYVTQQAGTGDRYVHGARSRFFSERVLGRFDRVSAPGARDEHHARAA
                     TGPRLDVASRLRDFW"
     gene            complement(4198..4572)
                     /locus_tag="VD737_02505"
     CDS             complement(4198..4572)
                     /locus_tag="VD737_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HXW09457.1"
                     /translation="MTKIYVGNLPFTATEQDVKELFGQHGTVESVALPTDRETGRPRG
                     FGFVEMPQADAQRAMGALNGFNMGGRPLRVNEAQDRPRTGGGGGGGRMGGGGGRPGGG
                     YGGGGGGGGYGGGGGGGGQRRW"
     gene            5257..5838
                     /locus_tag="VD737_02510"
     CDS             5257..5838
                     /locus_tag="VD737_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HXW09458.1"
                     /translation="MEAFLISTGIVALAEIGDKTQLLALVLAARFRKPVPIILGILVA
                     TLVNHAMAGAAGAWIAAAVGPTAMRWILGLSFIAMAIWTLKPDELDDEAADERRAAAS
                     RMGVFGTTLVAFFLLEMGDKTQIATVALAAKYASLASVVLGTTLGMMLANVPAVLLGE
                     VAARKLPMQLVHRIAAALFLVLGVTVLLGFGSG"
BASE COUNT          971 a         2088 c         2049 g          894 t
ORIGIN      
        1 cgacctcgac gtggcggcga gtcgcatcgc ctggggcaag ttcatcaatg cggggcagac
       61 ctgcgtggcg cccgaccacg tgctggtgca ccgcgaggtc gccgcgcagt tcgccgacac
      121 gctcgcggcg aagatcaagc agttctacgg agacgatccg gcgcagagcc ccgacttctg
      181 ccgcattgcg agcgaacgtc acacggcgcg cttcgcgaag ctgctcgaag gccagaagat
      241 ccacagcggc ggacaggtcg acgtcgcgcg tcgctacgtc gccccgacga tcgtgctcga
      301 tccggatccc gaatcggagc tgatgcgcga ggagatcttc ggccccgtgc tgccggtgat
      361 caccgtggac gaaatgcacc acgcgatcaa gttcgtcgcc gaccggccga agccgctggc
      421 gttgtacctg ttctcgaaga gcaaggcgct cgaggacgcg gtgctgtcgc ggttgagcgc
      481 cggcagcgtc tgcatcaatg acgcggtgat cttcatggtg tcgcccgagt tgccgttcgg
      541 cggcatcggc gcgagcggca tgggccggta caccggctgg tacggcttcg agacgttcag
      601 ccacatgaag cccgtcatga agcgcagctt ccgcttcgac gcgccgctgc ggtatccgcc
      661 ctacgacgac ttcaaggcga aggtcatgcg cctggtgcgc tgagcaaccg cgtggatccg
      721 ctggatcccg acctgctgga catggcgctc cggatcctgg tctcgctggc cgccggggcg
      781 ctgatcggtt acgaacgctc gtatcgcggc cgtcccgcgg gattccgcac gcacgcgctc
      841 gtctgcatgg catcgacgct gctgatgctg ctgacggtct acgagtcgca ctgggtcgag
      901 acgcccgacg cgctggtgcg gctggacccg acccgcatgg cccagggcat catgaccggc
      961 atcggcttcc tcggcgccgg cgtgatcatg cgcgagggcc tgtcggtgcg cggcctgacc
     1021 actgcggcgt cgatctggat cacggccgcg atcggcgtgc tcgcgggcgc gggtttctat
     1081 ttcccgctcg tgctggccgt ggccctcacg ctcggcgtgc tgtcgctctt caggctgatc
     1141 gaagtgcgca tgccgacgca ggcgtactac cacttcgacg tgcggttcgc gccggacgcg
     1201 acgcacggcg agcagaccgt ccgcgcgctc gtcgcaaagc acggcttcac cgtcgccaac
     1261 ttcagttacc ggctggacga cgaggggcgc gtgcgtcgct acacgacgac gatcgcctcg
     1321 accgaccgct cggcggccgg tcgtctggcg atcacgctgg accagcacgg cgacgtgcac
     1381 gaataccgga tcgcgccgac cagcgactga accggccgct gcctcaggcg tcgacgcgat
     1441 cgaccgaggc ctgcccggaa atccgcgccc gcaggtgcgc ccacgacatg ccgatcccga
     1501 gcagcaggcc gaggcaggtc agcacgaccc acgacatcgc cgaccggttc tcaaccgaca
     1561 gccagccctt cgagacgatc caccacacga tcgacgcgaa cagcgcgagc agcagcacga
     1621 cgcctacggc gcccatcgac gagaacgtcg cccgcacgaa gaacagccag gcgcccagca
     1681 gcgcgatgcc ggccaccacg accagcggat cgatcggcga gaggtggctc gacagccagt
     1741 ggtagtaaga cagtccggtc ggattgaacg tcaggaagac gagtgccagc gagaaagcga
     1801 cccgcacgag cacccccgtg acgcggaagt cggtgccgtc cgtcgatgcc acgctcgctc
     1861 cccctggtca cgcgtcgcgg cattctaggc gaatcgcgtc gagccgccgc cggtctcgta
     1921 tactgtctat ccatacagtc ccacggccat gaacgcgatc gccatacccg cgccgcacgc
     1981 cacgctgaac cccgcccagg cggaggcggt cacgcacggc cagccccgct ccggcggcgg
     2041 cgtggccgcc ggcccgctgc tcgtgatcgc cggggcgggc accggcaaga ccaacacgct
     2101 ggcccatcgc gtcgcgcacc tgctgctgca gggggtcgcc cccgagcgga tcctgctgct
     2161 gacgttcacc cgtcgcgcag ccctcgagat gacgcggcgc gcgcagcggg tgatggcgca
     2221 ggcgctcgcg gcccgcgggc ccggtcacgc gacggtgccg gagaacctgc ggctgtcgtg
     2281 gtcgggcacg tttcacgcga tcggcaaccg gctgatccgc gagtacgcac cgcggctcgg
     2341 cctcgatccc gggttcacgg tgctcgatcg cggcgatgcc gccgacctgt tcgacgtcgt
     2401 gcgtcaccgg ctcgggctgt cgaagaccga aaagcgcttt ccgcgcaagg acatctgcct
     2461 cgcgatctac tcgcaccgga tcaacacgca gcggccgctg cggcacacgc tcgaaaccgt
     2521 ttacccgtgg tgcctcgact gggagcagga gctcacttcg ttgtgccgtg cgtacgtgga
     2581 ggcgaagctc gagcagcagg tgctcgacta cgacgacctg ctgctgtact ggcacgtgat
     2641 gacgcgcgac ccggcgatcg ccggtgaact cggcgcccgc ttcgatcacg tgctcgtcga
     2701 cgagtaccag gacaccaacg tgctgcaggc cgagatactg ctgcacctga agccggacgg
     2761 cgcggggctc acggtggtcg gagacgacgc gcaggccatc tattcgttcc gcgcggcgtc
     2821 cgtggacaac atcctggaat ttccgcagaa gttcacgccg ccggcgcgcg tcgtcacgct
     2881 ggagcagaac taccggtcca cgcagggcgt gctcgactcg gcgaacgcgc tgatggacga
     2941 ggccggtcgc cagtactcca agcagctgcg cagcgcacgc gggccgggcg cgcggccgca
     3001 gtacgtcacc gtgctggacg agcaggcgca ggcgacgttc gtgatcgacc gcgtgctcga
     3061 agcgcgcgag cgcggcgtgg acctgcgacg ccaggccgtg ctgttccgca actccgacca
     3121 cagcgacgtg ctcgagctcg agctggtgcg tcgcaacgtg ccttacgtga aatacggcgg
     3181 cctcaagttc ctcgaggcgg cccacgtcaa ggacctgctg gcggtgctgc gctgggccga
     3241 caatccgcgc aaccggatcg ccgcgttccg cgtgctgcag ctgctgccgg gcatggggcc
     3301 ggccaacgcg cagcgggctt gcgaggcatt cgaggccgca tcgttcgcct ggacggcgct
     3361 ggcggcccac gcggtgccgg cagccgcacg cgacggctgg gcggcgctcg tcgcgctgct
     3421 cgtccagatc ggggcggacg gcgcgccctg gcccggccag gtcgagcgag tgcgtcgctg
     3481 gtacgagccg cagctcgagc ggatctacga gtccgcacag gcgcgcaagg gtgacctgga
     3541 ccagctcgac cggctgagcc agcagcacgc gtcgcgggag tcgttcgtca cggagctcac
     3601 gctcgacccg ccgcaggcca cgggcgacct gtcgggcgac ccgctgctcg acgaggatta
     3661 cctggtgctg tcgaccgtgc actccgccaa ggggcaggag tgggacaccg tccacgtgct
     3721 gaacgtgacc gacggcagct tcccatcgga gttcgcgagc ggccgcacgg agttgctcga
     3781 ggaggagcgg cgactgctgt acgtcgcgat gacgcgcgcg aaggacgagt tgcgggtggt
     3841 cgcgccggtg cggttctacg tgacgcagca ggcgggcacg ggagaccgat acgtgcacgg
     3901 cgcccgcagc cgtttcttca gcgagcgcgt tctcggtcgc ttcgaccgcg tcagcgcccc
     3961 gggagcgagg gacgagcacc acgcgcgcgc ggcgacgggc ccacgactgg acgtcgcgag
     4021 tcgtttgcgg gatttctggt aaacgccgat cccgccaggc acaggcgcgg gcctgtgcct
     4081 cgtggatggc acttaggacg cccgaagacg gatgaccgcg accgtccggt cgcggtcatc
     4141 ccctgggcat gcaggtgctg acggggccgg gcccgaaggc ttcagccccg cgcggagtca
     4201 ccagcggcgc tgaccgccgc cgccaccgcc gccgccgtag ccgccaccac caccgccgcc
     4261 gccgtagccg ccgcccggac ggccaccgcc gccgcccatg cggccaccac cgccgccgcc
     4321 gccggtgcgc gggcgatcct gagcttcatt cacgcgcagc ggacggccgc ccatgttgaa
     4381 tccgttcagg gcgcccatcg cgcgctgggc gtccgcctgc ggcatttcga cgaagccgaa
     4441 gccgcgcggc cggccggtct cgcggtcggt cgggagggca acggactcga cggtgccgtg
     4501 ctggccaaac agttccttga cgtcctgctc cgtcgcagtg aaaggcaggt tgcccacgta
     4561 gatcttggtc attcgaacat cctcagagat aacacatcaa accggtccca ggcgtcattg
     4621 cctggccggc actccaacaa ctgaaacaaa acaacaggag gattcgcagc cgagcgtctg
     4681 acggtcgccg gtctggccgg cgccgcggcg acgtgtcctg tcacctgatc gaagacgttt
     4741 cggctaacaa aggtggcgca tggaagcgcc ctgcaagaca cgtacgggca acgatcgccg
     4801 cgcatcgtac cacaggcctg gcccgtccag cgtcgaccgg ctcgcggtcg cccggcggac
     4861 gcccggcgcc gggtcggcgc gggtcgtcgg ggcaggccac gctgcgccga tccttggcgg
     4921 agaagggaca cgtgtccctg gggcaggagc gtcatgaaat ggagggcacc gcgcggaaag
     4981 gatggatcta aggccggact gttgtctgct tctgggccga cgtcgcgcga tgcgcacaat
     5041 ggtcaaaact tgaccagtgc tggaatgtac ctacggttgg ctcccgctgc aacttaattc
     5101 gttgacgcgg cgcaaaaatc gtgcgcccgc cgacgagcgc gcgggatacc gcctgcagcg
     5161 tcgcgtcgtg cagtcgccac ccggggaacg acgccgccgg gggtggtgta gagtgcgcgc
     5221 gccgtcaggt ccggcccgcc cgacgaaccc gccgccatgg aagctttcct gatctcgact
     5281 ggcatcgtcg cgctcgccga aatcggcgac aagacgcagc tgctcgcgct ggtgctggcg
     5341 gcgcggttcc gcaagccggt gccgatcatc ctcggcatcc tcgtcgcgac gctcgtcaat
     5401 cacgcgatgg ccggcgcggc aggcgcgtgg attgccgccg cggtcggccc caccgcgatg
     5461 cgctggatcc tcggactctc tttcatcgcc atggcgatct ggaccctgaa gccggacgag
     5521 ctcgacgacg aagccgccga cgagcgccgg gcagccgcgt cgcgcatggg cgtgttcggc
     5581 acgacgctcg tggcgttctt cctgctcgag atgggcgaca agacgcagat cgcgaccgtg
     5641 gcgctcgcgg cgaaatatgc ctcgctcgcc agtgtcgtgc tcggcaccac gctggggatg
     5701 atgctggcca acgtgccggc ggtgctgctc ggcgaggtcg ccgccaggaa gctgccgatg
     5761 cagctcgtgc accggatcgc ggccgcgctg ttcctggtgc tgggggtgac ggtgctgctg
     5821 ggcttcggaa gtggctagtg gctagtggca gcgagtggct agtggctagt ggctagtgaa
     5881 gagctgcgcg gcgcgccgac gcagtcggct tcctaccagc cactgaccac tgaccactaa
     5941 ccactaacca ctacccacta accactaacc actgaccact gaccactgac cactttcctc
     6001 ac
//
LOCUS       DASPFR010000100         5958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_640104, whole genome shotgun sequence.
ACCESSION   DASPFR010000100 DASPFR010000000
VERSION     DASPFR010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640104"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VD737_02515"
     CDS             <1..815
                     /locus_tag="VD737_02515"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXW09459.1"
                     /translation="PGKKSDLVPEGCTVHTLAGPTQDVQGSLDELAARVGASGREPAR
                     QSPMRPDRPQGKFTADKICKAIGHLLPERAIVVDEAQTSGVMLPMMTSGAPRHDVLTL
                     TGGAIGQGLPCAVGAAIACPDRPVLALVGDGSAMYTIQALWTMAREKLDVVAVIFNNR
                     SYAILNVELQRVGAGEAGGKARSQLDLADPALDFVHMARGMGVPAVRATTTDEFVLAL
                     DAAFRTPGPHLIDAVVPSAISGLKLRLLPHVLGSLQRLPASLGRTIKRKLAP"
     gene            1063..1707
                     /locus_tag="VD737_02520"
     CDS             1063..1707
                     /locus_tag="VD737_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09460.1"
                     /translation="MNTRKLALLVALAVGASGTIAAPAHAESSIARSVVLATPKDEVP
                     ARADERVEIRSLHRASTILLQTTLRQRPNSPDWLMHEAGSKPLVVTRSPDPRSRYLAL
                     QVDRLDGTDQLMVRQALETFGNLHLYAGAGLGRAKYLDDDVLAKPLPRRRARHELAAA
                     AEIGAQAQLGTQLALDASVRWLDLGSDVTLLKSDYGPMNASEVMLGITVGYRFR"
     gene            1798..2730
                     /locus_tag="VD737_02525"
     CDS             1798..2730
                     /locus_tag="VD737_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09461.1"
                     /translation="MDPAIEPALAIDDVIERFLSESAFARDGAIVTDLDGTAVHEFDG
                     RIVIPKSVSHGLGELRRLHRPIVLNTLRFPLNVINTFGREWYDVSAAPLPLISLNGSV
                     VGHLREDSDGAIEFEELTSTAVPPATCEALVGELERLLASGIDDLVLFYYPHDWRAGE
                     RLWTPVEARQPRLLERYRSASAIDSVSLEQLAAALEACAPRMLFVLVGASHDELMAYQ
                     HARPNQFVTADGVDKASGAAVVAQRLGFDLASSVGAGDTMMDNFLSSVGLAVRVGNPS
                     LPYDGRHATVDLADSLALGEFWFRLADRVRRSAP"
     gene            2727..3647
                     /locus_tag="VD737_02530"
     CDS             2727..3647
                     /locus_tag="VD737_02530"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HXW09462.1"
                     /translation="MSGARREARLRRVARYIFEQAAGTAPGRIRVQEGGRTNHVFVLE
                     NPDGAFVLRMDEQDDKVHQYRKERWAIDHARRMDIPAPNVIEVGTTPDGVSFMLSRRE
                     PGRPATDRDDALAVLRQLGQYARKLHGETLGQFGGHQGGETAPWTGWEDFLRHEYDMD
                     RRLYQLLALGLLDEFTARRVADVVLRLGEGREPRLNHGDLRLKNVLVEEGSGRITAII
                     DWENAIAAPSPEWDLALALHDLSVDQKDAFLDGYGIEHEELVAARSTLAALNLLHYAP
                     FADDAARTGDEQRLRHYRRRFARLHDLYSP"
     gene            3647..4936
                     /gene="ligD"
                     /locus_tag="VD737_02535"
     CDS             3647..4936
                     /gene="ligD"
                     /locus_tag="VD737_02535"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HXW09463.1"
                     /translation="MPASRRKTAATARGEVEILSVGGRDVAVSNPGKVLFPTPGHTKL
                     DLARYYVAVAEGALRGAGGRPNMLVRYPDGVEGEHFYQKRAPGSRPEWIEVVELSFPS
                     GRTAEEVVPRDAAALAWIANLGCLELHPHPVRAEDLDHPDELRVDLDPVSGVTWPQIR
                     QVAHVARAVLEDVGLVGWPKTSGSRGIHLVVRIEPRWTFDEVRRAALALAREVELRAP
                     RLATSKWWKEERHGVFVDYNQNAKDRTVASAYSVRPKPDARVSAPVAWDELDACDPAD
                     FTLASMPARFAKLGDRHAGIDRHPGSIEELLELFERQGQGDAPWPPHYRRQASEPPRV
                     QPSRRKSTGRRKSTMPLVEIARARRKEDAEAGLGRWKARHPEAAEHLQPADVLVDSMR
                     GRYSTWTRIRVNLQHVPQDLRPAQEPLDPDEKIRSSS"
     gene            complement(4891..>5958)
                     /locus_tag="VD737_02540"
     CDS             complement(4891..>5958)
                     /locus_tag="VD737_02540"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HXW09464.1"
                     /translation="APPVEPMLSKAADEIPPEGGFLYEPKWDGFRAIVFRGSTDLYIQ
                     SRDSRPLDRYFPELHDTLIERLPKPCVLDGEIVIATGRGLDFDALQLRLHPAASRVEK
                     LAKQSPASFVAFDVLSIGNEDLTNARQSQRRAALEDLLGKVRPPVHLTPVTRDRTLAL
                     DWLTRFEGAGLDGVIAKPASGTYLPGKRAMIKVKHARTAECVVAGFRWYKGGQDSVGS
                     LLLGLYDDEGVLQHVGVTSSFTMAMREQLVRELAPLRKRAMDDHPWRDWAGAAAESSR
                     MPGAQSRWSSGKDLSWEPLRIERVCEVKYDHMQGDRFRHAAIFLRWRPDKPPRDCRYD
                     QLEVTRPYELERIFSSGSSGS"
BASE COUNT          913 a         2103 c         2092 g          850 t
ORIGIN      
        1 accccggcaa gaagagcgac ctggtcccgg aaggctgcac cgtgcacacg ctggcgggcc
       61 ccacgcagga cgtacagggc agcctggacg agttggccgc gcgcgtcggt gcgtccggac
      121 gcgagcctgc gcggcagtca ccgatgcgcc cggaccgccc gcagggcaag ttcacggccg
      181 acaagatatg caaggcgatc ggtcacctgt tgcccgagcg cgcgatcgtc gtggacgagg
      241 cgcagacctc cggcgtcatg ttgccgatga tgacctcggg cgcgccgcgc cacgacgtgc
      301 tcacgctcac gggcggtgcg atcgggcagg gcctgccctg cgcggtcggc gccgcgatcg
      361 cgtgtcccga tcgcccggtg ctcgcgctgg tcggtgacgg gtcggcgatg tacacgatcc
      421 aggcgctgtg gacgatggcg cgggaaaagc tcgacgtcgt cgcggtcatc ttcaacaatc
      481 gctcgtacgc gatcctcaac gtcgagctgc agcgcgtcgg cgccggcgag gccggcggca
      541 aggcccggag ccagctggac ctcgccgatc ccgcgctcga cttcgtgcac atggcgcggg
      601 ggatgggcgt accggcggtg cgcgcgacca ccaccgacga gttcgtgctg gcgctcgacg
      661 ccgcctttcg cacgcccggg ccgcacctta tcgacgctgt cgtgccgagc gcgatctccg
      721 gcctgaagct gcgcttgctg cctcacgtgc tggggtcgct ccaacggctg ccggcatcgc
      781 tgggacgtac gatcaagcgc aagctggcgc cctgatcctc gcggcctgaa gcgcggcgtt
      841 tccgaaaccc gccgctgccg ctgtcggaca gcgccggcct ggccggccga tcgtcgtaca
      901 tggcacggct gcgcgcctgc acgacgcggc gtgctcaccg tagagcgccc ctcaccggtc
      961 tgactgccgc cgacgcggct cccggcagac ccgcgctggc acctgcgcga tcgtgcgtca
     1021 ccgtcgaacc ccatcgggga accgcaacct gcaccggccg ccatgaacac acgcaagctg
     1081 gcactcctcg tggccctcgc cgtcggcgct tccggcacga tcgcggcgcc cgcccacgcc
     1141 gagtcctcga tcgccaggtc agtggtgctc gcgacgccga aggacgaggt gccggcgagg
     1201 gcggacgaac gcgttgaaat tcgatcgctc caccgggcct ccacgatcct gttgcagacg
     1261 acgcttcgac agcgccccaa ctcgccggac tggctgatgc acgaggcggg ctcgaagccc
     1321 ctcgtggtga cccgaagtcc cgacccgcgg tcccgctacc tggcactgca ggtcgatcga
     1381 ctcgacggca cggaccagct gatggtgcgc caggcgctcg agacgttcgg caacctgcac
     1441 ctgtacgccg gcgcgggact cggacgcgca aaatatctcg acgacgacgt gctcgccaag
     1501 ccgttgccga gacgccgggc acggcacgag ctcgcggcgg cggcggaaat cggcgcgcag
     1561 gcgcagctgg gcacgcagct cgccctcgat gcctcggtgc gatggctcga cctcggcagc
     1621 gacgtgaccc tcctgaagtc cgactacggc cccatgaacg cgagcgaggt catgctgggc
     1681 atcacggtgg gctataggtt ccggtagcac ccgcggccga cgttgcgggc ggtcgggcgc
     1741 gccgcgatgc ggcttcggcc gacaggggcc ctgcgcgagg gaacctagga ttccgccatg
     1801 gaccctgcga tcgagcccgc gctcgccatc gacgacgtca tcgagcgatt cctgtcggag
     1861 tcggcgttcg cacgcgacgg tgcgatcgtc acggacctcg atggcacggc cgtgcacgag
     1921 ttcgacggcc gcatcgtgat ccccaagagc gtcagtcacg gtctcggcga gctgcgccgc
     1981 ctgcatcggc cgatcgtgct caacaccctg cggtttccgc tgaacgtcat caacacgttc
     2041 ggccgcgaat ggtacgacgt gtcggcggca ccgctgccgc tcatctcgct gaacggcagc
     2101 gtcgtcggcc acctgcgcga agacagcgac ggcgccatcg agttcgagga actcacgtcg
     2161 acggccgtcc cgccagcgac ctgcgaggcg ctcgtgggcg agctcgaacg gctgctcgcg
     2221 agcggcatcg acgacctggt gctgttctac tacccgcacg actggcgggc cggcgagcgg
     2281 ctctggacgc cggtcgaagc gcgccagcca cggctgctcg agcgttatcg cagtgcgagc
     2341 gcgatcgaca gcgtatcgct cgaacagctc gccgcggcgc tcgaggcctg cgcgccccgc
     2401 atgctgttcg tgctggtcgg cgcaagccac gacgaactga tggcctacca gcacgcacgc
     2461 ccgaaccagt tcgtcacggc cgacggcgtc gacaaggcga gtggtgcggc cgtcgtcgcg
     2521 caacggctcg gcttcgacct cgcgtcgagc gtcggagccg gtgacacgat gatggacaac
     2581 ttcctgtcga gcgtcgggct ggcggtgcgc gtcggcaacc cgtcgctgcc ctacgacggc
     2641 cgacacgcca ccgtggacct tgccgattcg ctcgcgctcg gcgagttctg gttccggctg
     2701 gccgatcggg tgcgacggag cgctccgtga gcggtgctcg cagggaggcg cggctgcgtc
     2761 gcgtggcacg gtacatcttc gagcaggccg ccgggacggc gccggggcgc atccgcgtgc
     2821 aggaaggcgg ccgcaccaat cacgtcttcg tgctcgagaa ccccgacggc gcgttcgtgc
     2881 tgcgcatgga cgagcaggac gacaaggtcc accagtatcg caaggagcgc tgggccatcg
     2941 accacgcccg gcgcatggac atcccggcgc cgaacgtgat cgaggtcggc acgacgcccg
     3001 acggcgtctc gttcatgctg tcgcgtcgcg agccgggccg cccggcgacc gaccgtgacg
     3061 acgcgctggc agtgctcagg cagctcgggc agtacgcgcg caagctgcac ggcgagacac
     3121 tcggtcagtt cggcggccac cagggcggtg agacagcgcc ctggaccggc tgggaggatt
     3181 tcctgcggca cgagtacgac atggaccgac gcctgtacca gctgctggcg ctggggctgc
     3241 tcgacgaatt cacggcccgg cgagtggccg acgtcgtgct gcgactcggc gagggccgcg
     3301 agccgcgcct gaatcacggc gacctgcgcc tgaagaacgt gctcgtcgaa gagggttccg
     3361 gtcgcatcac cgcgatcatc gactgggaga acgccatcgc ggcgccgtct cccgagtggg
     3421 accttgcgct cgcgctgcac gacctgtcgg tcgaccagaa ggacgcattc ctggacggtt
     3481 acgggatcga gcacgaggag ctggtggcgg cccgctcgac gctcgcggcg ctcaacctgc
     3541 tgcactacgc gccgtttgcg gacgacgcgg cccggaccgg ggacgaacaa cgcctgcggc
     3601 actaccggag gcgcttcgcg cgcctccacg acctctactc gccgtgatgc cggcgtcgcg
     3661 tcgcaagact gccgcgaccg cccgcggcga agtggagatc ctcagcgtcg gcgggcgcga
     3721 cgtcgcggtc tcgaacccgg gcaaggtgct gttcccgacg cccggccaca ccaagctcga
     3781 cctcgcgcgc tactacgttg ccgtggctga gggcgcactg cgcggcgccg gcggccgccc
     3841 gaacatgctg gtgcgctatc cagacggcgt cgagggcgag cacttctacc agaagcgtgc
     3901 gcccggcagc cgaccggaat ggatcgaggt ggtggaactg agcttcccct cgggacgcac
     3961 ggcagaggag gtcgtccccc gggacgccgc ggcgctcgcg tggatcgcga acctcggctg
     4021 cctcgaactg caccctcatc cggtacgcgc cgaggacctc gatcaccccg acgaactgcg
     4081 cgtcgacctc gatcccgttt cgggggtgac gtggccgcag atccggcagg tcgcgcacgt
     4141 ggcgcgcgcg gtgctcgagg acgtcggtct cgtcggctgg cccaagacct ccggctcgcg
     4201 cggcatccac ctcgtggtgc ggatcgagcc ccgctggacc ttcgacgaag tgcgacgggc
     4261 cgcgctcgcg ctcgcgcgtg aagtcgagct gcgcgcgccg cggctcgcca ccagcaagtg
     4321 gtggaaggaa gagcgccacg gcgtgttcgt cgactacaac cagaacgcga aggaccgcac
     4381 ggtggcgtcg gcctattccg tgcggccaaa gcccgatgca cgcgtgtccg cgccggtcgc
     4441 ctgggacgag ctcgacgcct gcgaccccgc ggatttcacg ctggcctcga tgccggcgcg
     4501 cttcgcgaaa ctcggcgacc ggcacgccgg catcgaccgg cacccgggtt cgatcgaaga
     4561 actgctcgag ctgttcgagc gtcagggaca gggcgacgcg ccgtggccgc cgcactatcg
     4621 gagacaggcg tccgagccgc cgcgagtgca gccctccagg cgcaagtcga ccggccggcg
     4681 caagtcgacc atgccgctcg tcgagatcgc gcgggcccgg cgcaaggagg acgcggaggc
     4741 gggactcggg cgctggaagg cacggcatcc ggaggccgcg gaacacctgc agccggcgga
     4801 cgtgctggtc gactcgatgc gcggccgata cagcacctgg acgcgcatcc gcgtgaacct
     4861 gcagcacgtg ccgcaggacc tgcggcccgc tcaggagccg ctcgaccccg acgaaaagat
     4921 ccgctcgagc tcgtaggggc gggtcacttc gagctggtcg tagcggcagt cccgcggcgg
     4981 cttgtcgggt cgccagcgca ggaagatcgc cgcgtgccgg aagcggtcgc cctgcatgtg
     5041 gtcgtacttc acttcgcaga cgcgctcgat ccgcagcggc tcccacgaca ggtccttgcc
     5101 cgagctccat cggctctgcg cgccgggcat gcggctcgac tcggcggccg cgcccgccca
     5161 gtcgcgccag ggatggtcgt ccatcgcacg tttgcggagc ggcgccagct cacgcacgag
     5221 ctgctcgcgc atcgccatcg tgaaggacga agtcacgccg acgtgctgca gcacgccctc
     5281 gtcgtcgtag aggccgagca gcagcgagcc cacgctgtcc tggccgccct tgtaccagcg
     5341 aaacccggct acgacgcact cggccgtgcg cgcgtgcttg accttgatca tcgcgcgctt
     5401 gcccggcagg tacgtgcccg acgcgggctt ggcgatcacg ccgtcgagcc ctgcgccttc
     5461 gaagcgcgtc agccagtcga gcgcgagcgt gcgatcgcgc gtgacggggg tgaggtgtac
     5521 cggcggccgg accttgccga gcagatcctc gagcgcggcg cgacgttgcg actggcgtgc
     5581 gttcgtgagg tcttcgttgc cgatcgagag tacgtcgaag gcgacgaacg aggcgggcga
     5641 ctgcttcgcg agcttctcga cgcgagaggc ggccgggtgc agccgcaact gcagcgcgtc
     5701 gaagtcgaga ccccggccgg tggcgatcac gatctcgccg tcgagcacgc acggcttcgg
     5761 cagccgctcg atcagcgtgt cgtgcagttc gggaaaatac cggtcgagcg ggcgcgagtc
     5821 ccggctctgg atgtagagat cggtggagcc gcggaacacg atcgcgcgga acccgtccca
     5881 cttcggttcg tacaggaatc ccccttccgg ggggatctcg tcggcggcct tcgagagcat
     5941 cggctcgacc ggcggcgc
//
LOCUS       DASPFR010000101         3071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1739165, whole genome shotgun sequence.
ACCESSION   DASPFR010000101 DASPFR010000000
VERSION     DASPFR010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1739165"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="VD737_02545"
     CDS             <1..1384
                     /locus_tag="VD737_02545"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09465.1"
                     /translation="AGLSADHLAAAGDPGASAAYGRAAVDQLNRCRLERALAYAQRAT
                     ELAREPKDHAAARATTADVQLALGRAAEACDTYRDAIGLAVDADGRASGWLGLATALR
                     IVDRHDEALTALDHAERDLAPDDHRRHARLWTLRGNLHFPRGELDLCLDSHRRALDHA
                     RQGESLEDVVRAFGGIGDAQYQRGRMRSAAAEFRRCVELAEQNRMAGLRLAYLPMVAV
                     TDIYMGELDAAIATCEAALADAIEQRDRRALLLTEGVLGSARLQRGEYAVAHEKSLRC
                     LELARELGARRFEAENQVLEGLALFGLGETAAARRALEAAAAAAREVAATYCGPWAIV
                     ALALAAWEDEPRGRALLAEGARLLERGCVSHNHLEFHHYAMELCGRWRDWASVRRHAD
                     ALEAYTRDEPLPWVEIIVSAHRAIAQAGSKPGRAATRDVQGALREARRHQFLTLVPLL
                     EASLDTAAQA"
     gene            complement(1424..2968)
                     /locus_tag="VD737_02550"
     CDS             complement(1424..2968)
                     /locus_tag="VD737_02550"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HXW09466.1"
                     /translation="MLHVTRKAVLPVAIMLAVVGLVACGGGGGDGNPPPVSDTTPPTA
                     PGNLTAIVASPTTVRLEWTASTDAGTGVAGYRVYRDGSAEPVATVATTSYDDAALAPG
                     TTYAYAVRAYDAAVPSNVSPASATVSVSTPGDVPEEPLLSTEPAFPALPAFVDPVLAL
                     QAPDDGSTWYVVEQEGRVRAFANEASVGSARTFVDLRSRVTSGGERGLLGMAFHPAYP
                     ADPRVYLSYTTTAGGELVSRVSEFRTTDGGVTLDPDSERVLFTVVQPAANHNGGHVAF
                     GPHDGLLYVGMGDGGSGGDPWEPFGNGQRLTTLLGKMLRIDVDEAAGALPYRIPAGNP
                     YAANATCEDGSGAEPCPEIYAYGFRNPWRWSFDRMTAELWVGDVGQGEREEVDRVTAG
                     GNYGWRCFEGTRPFNSDCGPNVAVSLPPLVEYDHGTGESITGGYVYRGEAFPALQGRY
                     VFGDFITGRLWQVPRTVTPTRRVTAAEATATALGISSFAEGVDGELLAVDYFTGTLHH
                     VVALDR"
BASE COUNT          440 a         1108 c         1118 g          405 t
ORIGIN      
        1 cgccggcctc agtgccgacc accttgccgc ggccggcgac ccgggcgctt cggcggccta
       61 tgggcgcgcc gccgtggacc agctcaatcg atgccggctc gagcgcgcgc tcgcgtacgc
      121 gcagcgggcg acggaactcg cgcgcgagcc gaaagaccac gctgcggccc gcgcgaccac
      181 ggccgacgtg cagctcgcgc tgggccgggc cgccgaggcc tgcgacacct atcgcgacgc
      241 catcgggctc gcggtcgatg cggacggacg cgcgagcggc tggctcgggc tcgccacggc
      301 gctgcgtatc gtggaccggc acgacgaggc gctcaccgcg ctcgaccacg ccgagcgcga
      361 cctcgcgccg gacgaccacc gccggcacgc ccggctctgg acgttgcggg gcaacctgca
      421 tttcccgcgc ggcgagctcg acctctgcct cgactcgcac cgtcgcgcac tcgaccacgc
      481 gcgccagggc gagtccctgg aggacgtcgt gcgcgccttc ggcgggatcg gcgacgcgca
      541 gtaccagcgc gggcgcatgc gttccgcggc cgccgagttt cgccgctgcg tcgagctcgc
      601 cgagcagaac cggatggccg ggctgcggct cgcgtacctg ccgatggtcg ccgtcaccga
      661 catctacatg ggcgagctcg acgccgcgat cgcgacctgt gaagcggcgc tcgccgacgc
      721 gatcgagcag cgcgaccggc gcgcgctgct gctgacggaa ggcgtgctgg ggtcggcacg
      781 gctgcagcgc ggcgagtacg cggtcgcgca cgagaagagc ctgcgctgcc tcgagctcgc
      841 ccgtgaactc ggcgcgcggc gcttcgaagc cgagaaccag gtgctcgagg gactggcact
      901 gttcggactg ggcgagacgg ccgccgcgcg tagagccctc gaggccgccg cggccgccgc
      961 gcgcgaagtc gccgcgacct actgcggacc gtgggccatc gtcgcgctgg ccctggcagc
     1021 gtgggaggac gagccgcgcg gtcgggcgct gctcgccgag ggcgcgcgct tgctcgagcg
     1081 cggctgcgtc agccacaacc acctggagtt ccaccactac gccatggagc tctgcggccg
     1141 ctggcgggac tgggcgtcgg tgcggcggca tgccgacgcg ctcgaggcat acacgcgcga
     1201 cgaaccgctg ccgtgggtcg agatcatcgt ctccgcacat cgcgcgatcg cacaggccgg
     1261 ctcgaagccg gggcgcgccg ccacccgcga cgtgcagggc gccctgcgcg aggcccgccg
     1321 acaccagttc ctgacgctgg tgccgttgct cgaagcctcg ctggacactg ctgcgcaagc
     1381 atgacgcgcc gccgccggcg cgtcccaggc tgcgagccgg gcgtcagcgg tcgagtgcga
     1441 cgacgtggtg caaggtgccg gtgaagtagt cgaccgccag cagttcaccg tcgacgccct
     1501 cggcgaaaga cgagatcccc aacgccgtgg cggtcgcctc ggcggcggtg acgcggcggg
     1561 tcggcgtgac ggtgcgggga acctgccaga gccggcccgt gatgaaatcg ccgaacacgt
     1621 atcgcccttg cagtgccgga aacgcctcgc cgcgatagac gtaaccgccg gtgatcgact
     1681 cgccggtgcc gtggtcgtac tcgacgaggg gcggcaggct cacggcgacg ttgggcccgc
     1741 aatcggaatt gaacggacgc gtgccttcga agcatcgcca gccgtagttg ccgccggccg
     1801 tcacccggtc gacttcctct cgttcgccct gaccgacgtc gccgacccag agttctgccg
     1861 tcatgcgatc gaagctccag cgccacgggt tgcgaaagcc gtatgcgtag atctcggggc
     1921 agggctccgc gccgctgccg tcctcgcagg tcgcgttcgc cgcgtacgga ttgccggcgg
     1981 gtatacggta cggcaaggcg cctgccgctt cgtcgacgtc gatgcgcagc atcttgccga
     2041 gcagcgtcgt gagtcgctgg ccgttgccga acggctccca cggatcgcca ccactgccgc
     2101 cgtcgcccat cccgacgtac agcagaccgt cgtgcgggcc gaacgcgacg tgtccgccgt
     2161 tgtgatttgc cgccggctgc acgaccgtga acagcacgcg ctcgctgtcg ggatcgagcg
     2221 tgacgccacc gtcggtggtg cggaactcgg aaacccggga cacgagctcg ccgcccgccg
     2281 tcgtggtgta cgagaggtac acgcgcgggt ccgcggggta ggccgggtgg aaagccatgc
     2341 cgagcagccc gcgctcgccg cccgaggtca cgcgactacg caggtcgacg aacgtgcggg
     2401 cgctgccgac cgaggcctcg ttggcgaacg cgcgcacgcg cccttcctgt tcgacgacgt
     2461 accatgtcga gccatcgtcc ggtgcctgca gcgcgagcac ggggtctacg aaggccggca
     2521 gggccggaaa cgccggttcg gtcgacagca agggttcctc gggaacatcg ccaggcgtcg
     2581 agacgctgac cgtcgcggat gccggggaca cgttggacgg cacggcggca tcgtaggcac
     2641 gcaccgcata tgcgtacgtc gtgcccggcg cgagtgccgc gtcgtcgtac gacgtggtcg
     2701 cgaccgtcgc gaccggctcg gccgagccgt cgcgatagac gcggtagccc gcgacgcccg
     2761 tgcccgcgtc ggtcgacgcg gtccactcga gtcggaccgt ggtcggcgag gcgacgatgg
     2821 cggtgaggtt gccgggcgcc gtcggtggcg tcgtatcgga aaccggcggc ggattgccgt
     2881 cgcccccgcc gccgccgcag gcgacgagcc ccacgaccgc gagcatgatc gccaccggca
     2941 gcacagcctt gcgtgtcacg tggagcatgc ggcgatcgtc gacggcggcc gccggcacga
     3001 cgataagcgt ccgacgcgca ccggcgtcag cgcgttccac cgatcgcgaa agccggcgtg
     3061 ccgcgcgcgt c
//
LOCUS       DASPFR010000102         8708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_31090, whole genome shotgun sequence.
ACCESSION   DASPFR010000102 DASPFR010000000
VERSION     DASPFR010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8708
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31090"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VD737_02555"
     CDS             <1..642
                     /locus_tag="VD737_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09467.1"
                     /translation="AIITFGYFAVHLLMLVHAWFVKGERGFFWGWRSMVPQLKDVQDF
                     VANMKYFLYLGPRPKFDRFTYWEKFDYLAVFWGVAMIGVSGLMLWYPEFFSKFLPGWA
                     LNAAYIIHSDEALLATGFIFLFHFFHTHLRPEAFPMDPVIFTGSMPLERFKEERPLEY
                     ERMVANGTLDKHLVPAPEQSNLTAAYVFGTIAVVIGLACAAGIFVALWGLFFG"
     gene            781..2262
                     /locus_tag="VD737_02560"
     CDS             781..2262
                     /locus_tag="VD737_02560"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HXW09468.1"
                     /translation="MKSFISVISRSRLSLLGAAIAATALVLFVTLFVMELLGFEGGPY
                     LGILSYLILPLMVVVGVLLMPVGVWRAKKREARGEKEPPLPVVDFNKPATRRALATFG
                     LLSLVGVVVLAVGSFKAVHWMESVEFCGTACHTVMQPEYTAYLRSPHSRVACADCHIG
                     SGADWFVKSKISGSWQLIAVTFNLYPTPVPTPVHNLRPARDTCEQCHYPGKFSGDRLK
                     VRVHYAEDEQNSEMHNVLLVKVGGQHGRTSSGIHWHVDPGVTIRYLSDPTRETIYDVE
                     MTDKDGKKTVYKTKEEAPAGSEWRTMDCVDCHNRPTHIHRPPAVEVDMALDQGTIDKS
                     LPFIKREGLRAIQVEYPSHEAARDGIAKDIAGYYAQNYPDAPKPAVEQAGKALGDIYS
                     WNVFPAMKVTWNTYPNHIGHKDSPGCFRCHDRKHKSEDGAKISKDCDSCHTILAEDEA
                     DPAILRQIAGEEPTEAAAAGAATGSEPAATTTQVTIPEAGPAG"
     gene            2429..2944
                     /locus_tag="VD737_02565"
     CDS             2429..2944
                     /locus_tag="VD737_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09469.1"
                     /translation="MDDTRTAATDSGTTTPRFSLAECRQVSVCDLSGAERFVVWAIRW
                     RASRDAGDALADSCLEDAFDRAGLSAVQPAFERFVVAACPHATTCRAADRLGCWRLQP
                     LEAHALHAIACLQAGLLGEAWKALARVCARREVGRALLQLEELASALDRIGGRIERWA
                     FTPSAMEPAIA"
     gene            complement(2986..3261)
                     /locus_tag="VD737_02570"
     CDS             complement(2986..3261)
                     /locus_tag="VD737_02570"
                     /inference="COORDINATES: protein motif:HMM:NF042692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6587 family protein"
                     /protein_id="HXW09470.1"
                     /translation="MLETILVSLIVLVAAAYAAWALLPAPTRRNLALKGARALGGPES
                     PGSRGRLAGVLQRLANARTGGCNDCPAATLTPAERTQPRREPQDRSR"
     gene            complement(3272..5146)
                     /gene="feoB"
                     /locus_tag="VD737_02575"
     CDS             complement(3272..5146)
                     /gene="feoB"
                     /locus_tag="VD737_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HXW09471.1"
                     /translation="MSIHAATIDPMRLALVGVPNCGKTALFNRLTGSRQRVANYAGVT
                     VERKEGQFVGPARQRLYKVLDLPGAYSLTPTTLDEAITRDFLLGRLADEPAPEFVVCV
                     VDATNLRLNLRLVLELRDLGVPMVVALNMSDLAIERGYSVDSVALERELGVPVVETVA
                     VEHGGVRHLVEKLEALPHPGSRTPVQWREPSFDQIQQTQREVRRILHAIDYTEPLRKQ
                     ALARLDALVMHPVAGPAILAFVLFFVFQAVFSWAAVPMDFIDGSVAQLGTFVQGALPD
                     GALRSLLVDGVIAGVGSVLIFLPQILILFFFILALEDSGYLPRAAYLLDRLMGSVGLS
                     GRAFIPLLSSFACAVPGIMATRTIPNLRDRLATIMIAPLMTCSARLPVYALVIAAFIP
                     SNPIGPFNLQGVVLFTLYFAGILSAMAVAFVLKRTSLRTAYHPLLLELPEYHWPHLNN
                     LVIGLWERTKVFLQRVGTIILAIMIVLWFLSTYPAPPPGAEGPAIVHSFAGMIGRALE
                     VVFAPLGFNWQISLALVPGLAAREVAVGALGTVYSLSTEGDVTGALVPMIAQTWSLAT
                     ALSLLAWYVFAPQCLSTLVVVKRETNSWRYPLIMAGYLFALAYLAAFVTYQVANALLG
                     "
     gene            complement(5160..5429)
                     /locus_tag="VD737_02580"
     CDS             complement(5160..5429)
                     /locus_tag="VD737_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HXW09472.1"
                     /translation="MPPVSLATLRKGARGVVVDVRDDAQSLGDEAQSTVSRRLLELGF
                     VPGEPVEVVQEIWPGGDPMAVRLGNTTFALRRREASAVMVEPESA"
     gene            5667..6242
                     /locus_tag="VD737_02585"
     CDS             5667..6242
                     /locus_tag="VD737_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXW09473.1"
                     /translation="MSESVLAGVARGDGPAMRECMERFGGLVWAIARRMTRSRADAED
                     AVQEVFVDVWRSAARYDPAQGTERVFIATIARRRLIDRIRRGRLMQYTSDESVLEDVR
                     FAEPGNHGEVRVEAERAARAVARLRPQQQRILRMGLLEGMTHSEIASATGLPLGTVKT
                     LMRRGLIQAREWLGVDAAVARSAAGLHCAAG"
     gene            6322..7215
                     /locus_tag="VD737_02590"
     CDS             6322..7215
                     /locus_tag="VD737_02590"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HXW09474.1"
                     /translation="MVRPASFGFNPETGGSNRFQRQEADLVADAQTLAQHEFDGLVAA
                     LRDAGVTVVTVEDEAVPRCPDAIFPNNWVSFHADGTVVLYPMLARSRRAERRYEHLYA
                     LERATGRRVCRVVDLSHHELEGRYLEGTGSVVFDHPARVAYAALSPRTHREPLAELCA
                     ELGYRPCVFRAADRDGVAIYHTNVMLAIGSGFAVIAIETVERSQRDDVLRELLQAGRA
                     VIEIDGRQMASFAGNVLELRAADGARVLAMSSAAAQAFGASLDTLGRHVDRVVTAAIP
                     TIETLGGGSVRCMLAEVFLPE"
     gene            complement(7384..7734)
                     /locus_tag="VD737_02595"
     CDS             complement(7384..7734)
                     /locus_tag="VD737_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXW09475.1"
                     /translation="MGKLGQDFAAYRERMNARILEEDNRVIKRVYSVDSLAYSEEAGG
                     ELPRQTKELLGLVASLVLRCDDCVKYHLVEARRAGNSRQSMVEAMSIGLVVGGTIVVP
                     HLRRAIEFLDDLEE"
     gene            7839..8480
                     /gene="plsY"
                     /locus_tag="VD737_02600"
     CDS             7839..8480
                     /gene="plsY"
                     /locus_tag="VD737_02600"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HXW09476.1"
                     /translation="MVELGFKTLIAYLLGSVIGSLLFGRLRGVDIRTQGSGNAGGTNA
                     LRTQGWAFALGVIVVDVGKALLAVGWLPSADLPGLHPDPSVDRDWLTVACATAVVVGH
                     VYPVWHEFRGGKGAATLVGTVLVLAPVALVPIVAVWLLTVMLTGYVGLGTMLGSATLP
                     VYLAVASPERTPLVVYGVLMSVFIVYTHRSNVRRMRAGSENRARRLWLLRPRG"
     gene            8477..>8708
                     /locus_tag="VD737_02605"
     CDS             8477..>8708
                     /locus_tag="VD737_02605"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HXW09477.1"
                     /translation="MNDTGARLLRLLADGHLHSGARLADALGVTRAAVWKSVGELRGK
                     GIDVASHDRRGYRLAAPVELLDAASLSRAAADA"
BASE COUNT         1407 a         2904 c         3040 g         1357 t
ORIGIN      
        1 gcgatcatca cgttcggcta cttcgcggtg cacctgctga tgctggtgca cgcctggttc
       61 gtcaagggcg agcgcggctt cttctggggc tggcgttcga tggtcccgca gctgaaggac
      121 gtgcaggact tcgtcgccaa catgaagtac ttcctgtacc tcgggccacg tccgaagttc
      181 gaccggttca cctactggga gaagttcgac tacctggcgg tgttctgggg tgtcgcgatg
      241 atcggcgtct cgggcctgat gctctggtac ccggaattct tctcgaagtt cctgccgggc
      301 tgggcgctca acgcggcgta catcatccac agcgacgaag cgctgctcgc gaccggcttc
      361 atcttcctgt tccacttctt ccacacgcac ctgcggcccg aagcgttccc gatggacccg
      421 gtgatcttca cgggctcgat gccgctcgag cggttcaagg aagagcgtcc gctcgagtac
      481 gagcgcatgg tggcgaacgg cacgctcgac aagcacctgg tgccggcacc ggagcagtcg
      541 aacctgacgg cagcctacgt gttcggcacg atcgccgtgg tgatcggcct cgcgtgcgcg
      601 gcgggcatct tcgtcgcgct ctggggcctg ttcttcggct gacgcgtcgc aaggctgcaa
      661 ggggccgacc tttcaaggtc ggccccttgc tcgtggaaaa ccgtcattgg ccccgcggga
      721 aagccgtata tcctcgcgcg ggatagactg cgtccatcgc aggaaggaaa acaggcagac
      781 atgaagagct tcatctcggt catctcgcgc agtcggctga gcctgctcgg agcggcgatc
      841 gccgcgacgg cgctggtact gttcgtcacg ttgttcgtga tggagctgct cgggttcgag
      901 ggtggaccgt acctcggcat cctgagctac ctgatccttc cgctcatggt ggtggtcggc
      961 gtcctgctga tgccggtggg cgtgtggcgc gccaagaagc gcgaggcgcg cggcgaaaag
     1021 gaaccaccgc tgccggtcgt cgacttcaac aagccggcga cccgccgggc gctggcgacc
     1081 ttcgggttgc tgtcgctggt cggcgtggtc gtgctggccg tcggcagctt caaggccgtg
     1141 cactggatgg agtcggtcga gttctgcggc accgcatgcc acaccgtcat gcagccggag
     1201 tacaccgcct acctgcgctc gccgcactcg cgggtagcct gcgccgactg ccacatcggt
     1261 tcgggggccg actggttcgt caagtcgaag atctcgggca gctggcagct gatcgccgtc
     1321 acgttcaacc tgtatccgac gcccgtgccg acgccggtgc acaacctgcg cccggcgcgc
     1381 gacacctgcg agcagtgcca ctaccccggc aagttctcgg gcgaccggct caaggtccgc
     1441 gtgcactacg ccgaggacga gcagaacagc gagatgcaca acgtcctgct ggtgaaggtc
     1501 ggcgggcagc acggccgcac ctcttcgggg atccactggc acgtcgaccc gggcgtgacc
     1561 atccgctatc tctccgaccc gacgcgcgag accatctacg acgtcgagat gaccgacaag
     1621 gacggcaaga agaccgtcta caagaccaag gaagaggcgc cggcgggttc cgagtggcgc
     1681 acgatggact gcgtcgattg ccacaaccgg ccgacgcaca tccatcgccc gcccgcggtc
     1741 gaagtcgaca tggcgctcga ccaggggacg atcgacaagt cgctgccgtt catcaagcgc
     1801 gaggggctgc gcgccataca ggtcgagtat ccctcgcacg aggcggcccg cgacggtatc
     1861 gccaaggaca tcgcgggcta ctacgcccag aactacccgg acgccccgaa gcctgcagtc
     1921 gagcaggcgg gcaaggcgct cggcgacatc tattcgtgga acgtgttccc ggcgatgaag
     1981 gtcacctgga acacgtatcc gaaccacatc ggccacaagg attcgccggg atgcttccgg
     2041 tgccacgatc gcaagcacaa gagcgaggac ggcgcgaaga tcagcaagga ctgcgacagc
     2101 tgccacacga tcctggccga ggacgaggcc gatccggcca tcctgcggca gatcgcaggc
     2161 gaagagccga ccgaggctgc cgccgccggt gcggccacgg gcagcgagcc ggccgcgacc
     2221 acgacccagg tgacgatccc ggaggcgggg cccgccggct gacccgattc gtgtccacgc
     2281 cgacggggcg cgagctcaac tcgcgccccg tctgcattcc gggggcagtc gcgtggtgac
     2341 cgccgccgac gcctggcgaa ggctgtcgat gggccgcata ggtgttttcc taattgagaa
     2401 tgattctcaa ttgccttaga gttgaggcat ggacgacacc cgcaccgccg ccacggattc
     2461 cggcacgacg accccccggt tcagccttgc cgagtgccgt caggtctcgg tctgcgactt
     2521 gagcggcgcc gagcgcttcg tcgtctgggc gatccgctgg cgcgcctcga gagacgccgg
     2581 ggacgccctg gcggactcat gtctcgagga cgctttcgac cgcgcggggc tcagcgccgt
     2641 gcagcctgca ttcgagcggt tcgtggtggc ggcctgcccg catgcgacga cctgcagggc
     2701 ggccgaccgc ctcggatgct ggcgcctgca gccgctcgag gcgcacgcgc tgcacgcgat
     2761 cgcctgcctg caggcgggcc tgctcggtga ggcgtggaag gcgctggcgc gcgtctgcgc
     2821 gcgacgcgaa gtcggacgcg cgctgctgca gctcgaggaa ctcgcttccg cgctcgaccg
     2881 catcggtggg cgcatcgagc ggtgggcgtt cactccgtcg gcgatggagc cagcgatcgc
     2941 ctgaggccgg cctgtgcgtg agcgcgccgc cgcgccgtca ttcgatcagc gtgaccggtc
     3001 ctgcggctcg cgacgcggct gcgtgcgctc ggcgggcgtc aacgtcgcgg cagggcagtc
     3061 gttgcaaccg ccggtgcgcg cattcgcgag ccgctgcagc acgccggcaa gacgcccccg
     3121 tgagcccggg gactccgggc ctccgagcgc ccgggcgccc ttgagtgcca ggttgcgccg
     3181 cgtcggtgcc ggcagcagcg cccacgccgc atacgcggcg gcgaccagca cgatcagcga
     3241 caccaggatc gtctcgagca tgcgggcgtc ctcagccgag cagcgcgttc gcgacctggt
     3301 acgtcacgaa ggcggcgagg taggcgagcg cgaacaggta gccggccatg atcagcggat
     3361 agcgccagga gttggtttca cgcttgacga ccacgagcgt cgacaggcac tgcggcgcga
     3421 acacgtacca cgccagcagt gagagcgcgg tcgccaggct ccaggtctgc gcgatcatcg
     3481 gcaccagcgc gccggtcacg tcgccttccg tcgacagcga gtagacggtg ccgagggcgc
     3541 ccacggcgac ctcgcgcgcg gcgagtcccg gcacgagcgc aagcgagatc tgccagttga
     3601 agccgagcgg cgcgaagacg acctcgagcg cacggccgat catgcctgcg aagctgtgca
     3661 cgatcgcggg accttcggct ccgggaggcg gagccgggta cgtgctcagg aaccagagca
     3721 cgatcatgat cgccaggatg atcgtgccga cgcgctgcag gaacaccttg gtgcgttccc
     3781 acaggccgat caccaggttg ttcaggtgcg gccagtggta ctcgggcagc tcgagcagca
     3841 gcgggtgata ggccgtgcgc agcgacgtgc gcttcaacac gaaggccacc gccatcgccg
     3901 aaaggatacc ggcgaaatac agcgtgaaca gcacgacgcc ctgcaggttg aacggcccga
     3961 tcgggttcga cgggatgaat gccgcgatca cgagcgcata gacgggcagc cgcgccgagc
     4021 aggtcatcag cggcgcgatc atgatggtgg ccagccggtc gcgcaggttc gggatcgtgc
     4081 gagtcgccat gattcccggc acggcgcagg caaagctcga caggagcgga atgaaggcgc
     4141 gacccgagag gccgacgctg cccatcaggc ggtcgagcag gtaggccgcg cgcggcagat
     4201 agccgctgtc ctccagtgcg aggatgaaga agaacaggat caggatctgc ggcaggaaga
     4261 tcagcacgct gccgacgccc gcgatcacgc cgtcaacgag caggctgcgc agggccccgt
     4321 ccggcagcgc gccctgcacg aaggtgccga gttgcgcgac ggagccgtcg atgaagtcca
     4381 tcggcacggc tgcccagctg aacaccgcct ggaatacgaa gaacagcacg aacgcgagga
     4441 tcgcgggacc ggcgaccggg tgcattacca gggcgtcgag tctcgcgagc gcctgcttgc
     4501 gcagcggttc cgtgtagtcg atcgcgtgca ggatgcggcg cacttcgcgc tgcgtctgct
     4561 ggatctggtc gaacgacggc tcgcgccact gcaccggcgt gcgcgaaccc gggtgcggta
     4621 acgcttcgag cttctcgacg aggtggcgca cgccgccgtg ctcgacggcc accgtctcga
     4681 ccaccggcac gccgagttcg cgctccaggg cgacgctgtc gacgctgtag ccacgttcga
     4741 tcgctaggtc gctcatgttg agcgccacca ccatcggcac gccgaggtcg cgcagttcca
     4801 gcacgagccg caggttcagc cgcaggttgg tcgcgtcgac gacgcagacc acgaactcgg
     4861 gggctggctc gtcggcgaga cggccgagca ggaagtcgcg cgtgatcgcc tcgtcgagcg
     4921 tggttggcgt gaggctgtag gcgcccggca ggtccaggac cttgtacagc cgctgtcgcg
     4981 cagggccgac gaactggcct tccttgcgct ccacggtgac gcccgcgtag ttcgcgacgc
     5041 gctggcggct gcccgtgagg cggttgaaca gcgccgtctt gccgcagttc ggcacgccga
     5101 cgagggcgag gcgcatcgga tcgatggtgg ctgcgtggat gctcatcgcg gttgctgctt
     5161 caggccgact ccggctcgac catcactgcg ctcgcctcgc ggcggcgcag cgcaaaagtc
     5221 gtgttgccca ggcgcaccgc catcgggtcg ccgccgggcc agatttcctg cacgacctcc
     5281 accggttcgc cgggcacgaa gcccagctcg agcagtcggc ggctgacggt cgactgcgct
     5341 tcgtcaccga ggctctgcgc atcgtcgcgc acgtcgacga ccacgccccg ggcacctttg
     5401 cgcagcgtgg caagactgac ggggggcatt cgaactgcag gacgacgctt ggaggggagg
     5461 cgtgcagttt gttcttaatg agattgattc gcaatacggc aaacaccgat cggccggtga
     5521 cttgccgcaa gcctcccgca agcacctgat ggatgggtcg cccaggagtt ggggcggagc
     5581 tgtacagaac atgtacacag gatggacaca acggatatac tgcctgtaca gaagctcgac
     5641 caacacgcaa aggcgcggag aaacggatgt ccgaatcagt gctggctgga gtggcccgcg
     5701 gcgacgggcc cgcgatgcgg gagtgcatgg agcggttcgg cggcctcgtg tgggcaatcg
     5761 cccgccggat gacgcgctcg cgggcggatg cggaagacgc cgtccaggaa gtgttcgtgg
     5821 acgtctggcg cagcgccgcc cggtacgatc ccgcgcaggg gacggagagg gtcttcatcg
     5881 ccaccatcgc gcggcgcagg ttgattgacc ggatccggcg cggtcggctg atgcagtaca
     5941 cctccgacga gagcgtgctc gaagacgtgc gcttcgccga accgggcaat cacggcgagg
     6001 ttcgcgtcga ggcggagcgc gccgcacgag cggtggcgcg attgcggccg caacagcaac
     6061 gcatcctgcg catggggctg ctcgaaggca tgacccattc ggagatcgcc tccgccaccg
     6121 gactgccgct cggcaccgtc aagacgctga tgcgtcgggg cctgattcag gctcgcgagt
     6181 ggctcggtgt cgacgcagcc gtcgccaggt ctgccgccgg gttgcactgc gccgcgggct
     6241 gagcccctag aatcgggggg catgacacag ccggcatctc caggaccagc gccgggcgcc
     6301 caggcggcga acgcggtgtt gatggttcgg ccggcgagct tcggcttcaa tccggagacc
     6361 ggcgggagca atcggttcca gcgccaggag gccgacctcg tcgcggacgc gcaaaccctg
     6421 gcgcagcacg agttcgacgg gctcgtcgcc gcgctgcgtg acgctggcgt gacggtcgtg
     6481 acggtcgagg acgaggccgt tccccggtgc cccgacgcga tctttcccaa caactgggtc
     6541 agcttccacg ccgacggcac cgtcgttctc tacccgatgc tcgcgcgcag ccggcgggcg
     6601 gagcgccgct acgagcatct ctacgcgctg gagcgggcga ccggacgtcg ggtctgccga
     6661 gtcgtggacc tgtcgcatca cgaactcgag ggccggtacc tcgaaggcac cggcagcgtc
     6721 gtgttcgacc atccggcgcg cgtcgcttac gcggccctgt cgccccgcac gcatcgcgag
     6781 ccgctcgcgg agctgtgcgc cgagctcggc taccgcccgt gcgtgttccg tgccgcggat
     6841 cgggacggcg tcgcgatcta ccacaccaac gtcatgcttg cgatcggcag tggctttgcg
     6901 gtgatcgcga tcgagaccgt cgagcggtcg cagcgggacg acgtattgcg cgaactgctg
     6961 caggcgggcc gcgcggtcat cgagatcgac ggtcgccaga tggcgtcgtt cgccggcaac
     7021 gtgctggagc tgcgcgcggc ggacggtgcg cgcgtgctcg cgatgtcctc ggctgccgcg
     7081 caggcgttcg gggcgagcct cgacacgctg ggtcgccacg tcgaccgggt cgtcacggcc
     7141 gcgatcccga cgatcgagac gctcggcggc ggctccgtcc gctgcatgct cgccgaggtc
     7201 ttcctgccgg aataggggac attccggaat tcccgctgat tggggacact ccggattaac
     7261 agaaaaggcc ggcaaaaata gcgccggccg aggaccgtcc gcgatcaccc gtcagcgccg
     7321 gcccgtgaaa tccagcacga aaaacaggag tgtccccatt taagcggaat tccggagtgt
     7381 cccctattcc tcgaggtcgt cgaggaactc gatcgcgcga cgcaggtgcg gcaccacgat
     7441 cgtgccgccg accaccaggc cgatgctcat cgcctcgacc atcgactggc gcgagttgcc
     7501 ggccctgcgg gcctcgacca ggtggtactt cacgcagtcg tcgcagcgca ggacgagcga
     7561 ggcgacgagc ccgagcagct ccttcgtctg cctgggcagc tcgccgcccg cctcctccga
     7621 gtacgcgagc gagtccaccg agtacacgcg cttgatcacg cgattgtcct cctcgaggat
     7681 tcgcgcgttc atgcgctcgc gatacgccgc gaagtcctgc ccgagcttgc ccatggtgcc
     7741 tccgacctcc gttcgtgggg tcgtcgagtg tacggtcgcg cccgggcggg ctcgaaggcc
     7801 gatgctagaa tcgcgccgcg gcgcgctgcg gaggtccctt ggtcgaacta ggcttcaaga
     7861 cgctcatcgc gtacctgctc gggtcggtga tcggcagcct gctgttcggc cgcctgcgcg
     7921 gcgtggacat ccgcacgcag ggcagcggca atgcgggtgg caccaacgcg ctccgcacgc
     7981 agggctgggc gttcgcgctc ggcgtgatcg tggtcgacgt cggcaaggca ctgctcgcag
     8041 tcgggtggct gccgagcgcc gacctgcccg ggttgcaccc cgacccctcg gtcgatcgtg
     8101 actggttgac cgtcgcctgc gcgacggcgg tcgtcgtcgg gcacgtgtat ccggtctggc
     8161 acgagttccg cggcggcaag ggcgcggcca cgctggtcgg gacggtgctc gtgctggcac
     8221 cggtcgcgtt ggtgccgatc gtagcggtgt ggctgctcac cgtgatgctg accggctacg
     8281 tcgggctcgg cacgatgctc ggcagcgcca cgctgccggt ctacctggcg gtcgcttcac
     8341 cggagcgcac gccgttggtc gtctacggcg tcctgatgtc ggtgttcatc gtctacacgc
     8401 accgttccaa cgtgcggcgc atgcgcgccg gcagcgagaa ccgcgcgcga cggctctggc
     8461 tgctgcgacc gcgcggatga acgacacggg cgcgcgactg ctacgcctgc tcgcggacgg
     8521 ccacctgcac tccggtgcac ggctggcgga tgctctcggc gtcacgcgcg cggccgtctg
     8581 gaagagcgtc ggcgagttgc gcggcaaggg catcgacgtg gcgagccacg accgtcgagg
     8641 ctatcgcctc gccgcgccgg tggaactact ggacgcggct tcgctgagtc gcgctgcagc
     8701 cgacgccg
//
LOCUS       DASPFR010000103         5661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1496202, whole genome shotgun sequence.
ACCESSION   DASPFR010000103 DASPFR010000000
VERSION     DASPFR010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496202"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="VD737_02610"
     CDS             complement(<1..975)
                     /locus_tag="VD737_02610"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXW09478.1"
                     /translation="MDYYKLPNGLKVVLSHDATAPTAVVAVYYNIGFRIEPRDRTGFA
                     HLFEHMMFQGSQNLGKMEFIRLVQANGGILNGSTRFDFTNYFQIVPSNALETILWAEA
                     DRMRGLAITQENLTNQQGVVKNEVKVNVLNTPYGGFPWLDLPQYANTNWYNAHNFYGD
                     LEDLDAATLDDVKEFFDTYYAPNNAALVVTGDFDPAQTRAWIAKYFGGIESTPRPPLP
                     DISEPRQEKEKRASRPDPLAERPALAVGYHAPKRNTPEYFAMGMLDQILVQGKDSRLY
                     EALVQKHGFTGDVEGGINLLGNMLNVNGPVLWTAALYHDPGTSAEAILD"
     gene            complement(1256..1672)
                     /locus_tag="VD737_02615"
     CDS             complement(1256..1672)
                     /locus_tag="VD737_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09479.1"
                     /translation="MTEAPRSDEAGTGSRWRLVRDVVVFQVKLGMEALLDLTLIPVSL
                     AAAGLDFVCGHWRQPRWFHAVLRFGERCERRIDLWGVGPGASNVEVDALLRGIETVVR
                     QPGAGPRAIRELRRWAATRLAPGEGDDAGGPRDGAR"
     gene            1767..2843
                     /locus_tag="VD737_02620"
     CDS             1767..2843
                     /locus_tag="VD737_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXW09480.1"
                     /translation="MYRDPCRTAAAALLACLPVALGQAQEAVPVAVVQPRVAEVAEEL
                     RLTGTLTAERRARLSPRVDGLVARVRVDEGDRVQTGASLIELDATVATLALERVKAET
                     AQARARSEEAGRLVSEARRLVAERHLPRTELDRREAEAKLATASLAASSAAEREQAEL
                     VKRHTVPAPFAGVVARRLTDVGEWVARGTPVIELVATDRVRLDVQAPQERFAALRGDA
                     DVRVLADSLGGESSPGRIIARVPVSDPTARTFLVRVLVDDAEGRLLPGTSATAVIGLP
                     GARQALVVPRDALRPYPDGSHTVFVVRDAAGGGSTAVERPVRLGRGGAQVEILEGIER
                     ADRVVVRGNERLRNGQPVRVTEVR"
     gene            2846..>5661
                     /locus_tag="VD737_02625"
     CDS             2846..>5661
                     /locus_tag="VD737_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXW09481.1"
                     /translation="MFEAIIRRGTLQAVIVLIFCVLAVAAAIRMPVQMIPDLEVRTVA
                     IETLWPGATPQDVEKEILIEQERYLRTVPNLKRMVSSAETGRATIRLEFPFGVEINDA
                     LIRVNNALSQVPAYPENADEPRLSTSSFSENAFMYYRLVARDGNPLGLDLDLMQDFAE
                     DNVRPRMERVPGVARVDVSGGAPREIRIEVDAARLAERGITLVDLRDTVRARNRDASG
                     GDIDSGKRRYLVRTLGRFETVEDVSNLILARRGDSIVRLGDVAAVRLSHAELRSLSYT
                     AGQRTLNLSVRREAGSNVIEIKRALTPVVEDLNRGLLARSGLELRLVNDDVRYVQDSV
                     HNVFQNLLLGALLATGVMYLFLRSPQATLVGVLGIPVCTLAAFLGLLFAGRTINVISL
                     AGVAFAIGMTVDNTIVVLENIERLRRGGVERLQATVDGVREVWPAVLASTLTTVLVFA
                     PILFVEEEAGQLFSDIAIAISSAILASMAVAILIVPAAIARLGFSGRAGERRDGRIHA
                     SILRFVDNLLESPKRRRATALGLPAALLFLVVVLLPPAEYLPEGEEPKVFASMIAPPG
                     YNLTEMQGIAAILNTEFARLLEDDPERFARGETSFPALATVNLSVTSRGLRVVAEPKR
                     PADVDALMSAFNARFREFPGMRAFSSRGSIISSNDGGTRSVNVDIAGPDLAQLYRTAD
                     AAFRRAEALFDRPQVNSEPSSLSLDQPLLVVRPDWPRLAELGFTAQGFGFAVAALSDG
                     AYVDEFILGDDKIDIRVYGTAGGARDVDALRGSPLASPAGAVLPLESLAELRDTVDTD
                     VVRRVDGRRTVTLSIVPPRSVALETAVQRVEDELLPALQRDGLAPAGVSLEVSGAADQ
                     LDATRRSLGLNFVVATILSYLLLVAIFTHWGWPLMILTTVPLGLAGGVLGLALVNALG
                     LRLPFDMITMLGFLILLGTVVNNP"
BASE COUNT          815 a         1936 c         1966 g          944 t
ORIGIN      
        1 gtcgaggatg gcttccgcgg aggtcccggg gtcgtggtac agcgccgctg tccacagcac
       61 ggggccgttg acgttgagca tgttgcccag caggttgatc ccgccctcga cgtcgcccgt
      121 gaacccgtgc ttctgcacga gcgcctcgta gaggcggctg tccttgccct gcaccaggat
      181 ctggtcgagc attcccatcg cgaaatactc cggcgtgttc cgcttgggag cgtggtagcc
      241 gaccgccagc gcgggcctct ccgccagcgg gtcaggcctg gacgcgcgct tttccttctc
      301 ctggcgcggt tcggatatgt caggcagcgg cggccgcggc gtggactcga tgccgccgaa
      361 gtacttcgcg atccaggcgc gtgtttgcgc cgggtcgaag tcgcccgtga ccacgagggc
      421 cgcgttgttc ggcgcgtaat acgtgtcgaa gaactctttc acgtcgtcga gggtcgccgc
      481 gtcgaggtcc tcgaggtcgc cgtagaagtt gtgcgcgttg taccagttgg tgttcgcgta
      541 ctgcggcaga tcgagccacg ggaacccgcc gtagggcgtg ttcagcacgt tgaccttcac
      601 ctcgttcttg accacccctt gctgattggt caggttctcc tgggtgatgg cgagtccacg
      661 catccggtcc gcttccgccc agaggatcgt ctccagcgca ttcgacggga cgatctggaa
      721 gtagttggtg aagtcgaacc gggtcgagcc gttcaagatt cctccgttcg cctgcacgag
      781 cctgatgaac tccatcttcc cgaggttttg cgaaccctgg aacatcatgt gctcgaagag
      841 gtgagcgaag ccggtgcggt cgcgcggttc gatgcggaag ccgatgttgt agtacacggc
      901 cacgacggcg gtcggcgccg tcgcgtcgtg ggagagcacg accttgagcc cgttggggag
      961 cttgtaatag tccaccggta ccgtaaatcg cgacggcgat tgctgggctg cgggcggcgg
     1021 cctggactcg cctgcgatgg ctgtgtgaac gctcgccgcg aacgcgaggc agaaaattcc
     1081 gccgaggatc ttgaaacgag aaaggctgct catgggtgct cctgctcctg cgccgacttt
     1141 agggggaagc aggcggcgag gaaacgtctg cagacccgcg catccgcccg tcgtcgcagg
     1201 ccatgcacgg gtcggctgat ctctggcgac acgcggcgcc gtgggcgttc ggccgctacc
     1261 gggcgccgtc ccgcggtccg ccggcatcgt ctccctcgcc aggcgcaagt cgcgtcgcgg
     1321 cccagcgcct gagttcgcgg atcgcccggg gcccggcgcc cggttggcgt acgaccgtct
     1381 cgatcccgcg caggagtgcg tcgacctcga cgttcgaggc gcccggtccg acgccccaga
     1441 ggtcgatccg gcgctcgcat cgttcgccga agcgcagcac cgcgtggaac caacgcggtt
     1501 gcctccagtg accgcacacg aagtccaggc ctgcggctgc cagcgagacc ggaatcagcg
     1561 tgaggtcgag cagcgcttcc atgccgagct tgacctggaa cacgacgacg tcacgcacga
     1621 ggcgccagcg cgagccggtc ccggcttcgt ccgatctcgg tgcctcggtc attcgggctc
     1681 aactcccata aacgcaaaga ctacctccga ttcggagtgt cttttagact cgaacgtttg
     1741 ccggccgcgt tcgaggtgac cagccaatgt atcgagatcc ctgccggacc gcagcggcgg
     1801 cactgctcgc gtgcctgccg gtggcgctcg gccaggccca ggaggcggtg ccggtcgcgg
     1861 tcgtccagcc ccgggtggcg gaggtggcgg aggaactgcg tctcacggga acgctgacgg
     1921 ccgagcgccg cgcccggctc tcgccgcgcg tcgacggtct ggtcgcacgc gtgcgcgtcg
     1981 atgaaggcga ccgcgtgcag acaggcgcga gcctgatcga actcgacgcc accgtcgcca
     2041 cgctggcgct cgagcgtgtc aaagcggaga cggcgcaggc gcgggcacgg tccgaggagg
     2101 ccggacggct ggtcagcgag gcgcggaggc tcgtagccga gaggcacttg ccgcgtaccg
     2161 agctcgatcg gcgggaggcg gaggcgaagc tcgcgacggc ctcgctcgcg gcgagcagcg
     2221 ccgccgaacg cgagcaggct gagctcgtca agcgtcacac ggtgccggca ccgttcgcag
     2281 gtgtcgtcgc gcgtcggctc accgacgtgg gcgagtgggt agcgcgcggg acgcccgtga
     2341 tcgaactcgt cgcaacggat cgcgtgcgcc tcgacgtcca ggcgccgcag gaacgtttcg
     2401 cggcgctgcg gggcgatgcg gacgtgcgcg tgctcgccga ttcgctcggc ggcgaatcga
     2461 gcccggggcg catcatcgcg cgtgtccccg tcagcgatcc gaccgcgcgc acgttcctgg
     2521 ttcgcgtgct ggtcgacgat gcggagggtc gactgctgcc cggcacctcg gcgacggctg
     2581 tcatcggcct gcccggggcg cgccaggccc tcgtggtgcc ccgggacgcg ctgcggccct
     2641 acccggacgg cagccacacc gttttcgtgg tgcgcgacgc cgccgggggc ggcagcacgg
     2701 cggtggagcg acccgtcagg ctcggtcgcg gcggcgcgca ggtcgagatc ctcgagggaa
     2761 tcgagcgcgc tgaccgcgtg gtcgtgcgcg gcaacgagcg cctgcgaaac gggcagccgg
     2821 tgcgggtcac ggaggtccgc tgatcttgtt cgaggcgatc atccggcgcg gcacgctgca
     2881 ggcagtcatc gtcctgatct tctgcgtgct cgcggtcgcc gcagccatac gcatgcctgt
     2941 gcagatgatc cccgatctcg aggtgcgcac ggttgcgatc gagacactct ggccgggcgc
     3001 caccccccag gacgtcgaga aggaaatcct gatcgagcag gagcgttacc tgcgcaccgt
     3061 gcccaacctc aagcgcatgg tgtcctccgc ggagaccggg cgcgcgacga tccggctgga
     3121 attcccgttc ggcgtcgaga tcaacgacgc gctgattcgc gtgaacaacg ccctgagcca
     3181 ggtgcctgcc tatcccgaga acgcggacga accccgtctc tcgacgagct cgttctcgga
     3241 gaacgcgttc atgtactacc ggctcgtcgc gcgcgacggc aatcccctcg gtctcgacct
     3301 ggacctgatg caggacttcg cggaggacaa cgtgcggccg cgaatggagc gcgtgcccgg
     3361 cgtggcgcgc gtcgacgtga gtggcggcgc cccgcgcgag atccgcatcg aggtcgacgc
     3421 cgcgcgtctc gcggaacgcg gcatcacgct ggttgacctg cgcgacacgg tgcgcgcgcg
     3481 caaccgcgac gcttccggcg gcgacatcga cagcggcaag cgccgctacc tggtgcgaac
     3541 actgggccgg ttcgagacgg tcgaggacgt gagcaacctg atccttgcac ggcggggcga
     3601 ctcgatcgtc aggctaggcg acgtcgcggc cgttcggctc agccacgcgg aactgcgctc
     3661 gctctcgtac accgcggggc agaggaccct gaacctgtcg gtgcgtcgcg aggcaggctc
     3721 taacgtgatc gagatcaagc gcgcgctgac gcccgtcgtg gaggacctca accgcgggct
     3781 gctcgcacgc agcgggctcg agctgcggct ggtcaacgac gacgtccggt acgtgcagga
     3841 ctccgtgcac aacgtcttcc agaacctgct gctcggcgcg ctgctcgcga cgggtgtgat
     3901 gtacctgttc ctgcgctcgc cccaggcgac gctggtcggc gtgctcggca tacccgtgtg
     3961 cacgctcgct gcgttcctcg gcctgctgtt cgccggtcgc accatcaacg tgatctcgct
     4021 ggctggcgtc gccttcgcca tcggcatgac cgtcgacaac acgatcgtcg tgctcgagaa
     4081 catcgagcgg ctgcgtcgcg gcggcgtcga gcgcctgcag gcgacggtcg acggcgtgcg
     4141 cgaggtctgg ccggcggtgc tcgcctcgac gctgacgacg gtgctcgtat tcgcgccgat
     4201 cctgttcgtg gaagaggagg cgggacagct cttctcggac atcgcgatcg cgatctcgtc
     4261 cgccattctc gcctcgatgg ccgttgcgat cctgatcgta ccggcggcga tcgcacgcct
     4321 cgggttctca ggacgggccg gcgagcgtcg ggacggccgc atccacgcct cgatcctgcg
     4381 cttcgtcgac aacctgctcg aatcaccgaa acggcgtcgc gcgacggcgc tcgggctgcc
     4441 cgcggccctg ctgttcctgg tcgtcgtgct gctgccgccc gccgagtacc tgcccgaggg
     4501 cgaggagccc aaggtgttcg ccagcatgat cgcgccgccc gggtacaacc tcaccgagat
     4561 gcagggcatc gcggcgatcc tgaacacgga attcgcgcgg ctgctcgagg acgacccaga
     4621 gcgattcgcg cgtggcgaga cgtcctttcc cgcgctcgcg acggtcaacc tcagcgtgac
     4681 gtcacgcggg ctccgcgtcg tcgccgagcc gaaacgccct gcggacgtcg atgcgctgat
     4741 gtcggcgttc aacgcgcgct tccgcgagtt tcccggcatg cgcgcattct cctcgcgcgg
     4801 ctcgatcatc tcgagcaacg acggcggcac gcgcagcgtc aacgtcgaca tcgcgggtcc
     4861 cgacctcgcg cagttgtatc gtacggccga cgctgcgttt cgccgcgcgg aggcgctgtt
     4921 cgaccggcca caggtcaatt ccgagccgtc ctcgctgtcg ctcgaccaac cgctgctcgt
     4981 cgtgcgcccc gactggcctc gcctcgccga gctcggcttc acggcccagg ggttcgggtt
     5041 cgcggtcgcg gcgctcagcg acggcgcgta cgtcgacgaa ttcatcctcg gcgacgacaa
     5101 gatcgacatc cgcgtctacg gcacggccgg gggcgcccgg gacgtcgatg cgcttcgggg
     5161 cagcccgctc gcgtcgccgg ctggggcggt gctgcccctc gagtccctcg ccgagctgcg
     5221 cgacaccgtg gacaccgacg tggtccgacg cgtggacggg cggcgcaccg tgacgctgag
     5281 catcgtgccg ccgcgctcgg tcgcgctcga gaccgcggtc cagcgcgtcg aggacgaact
     5341 gctgcccgcg ctgcagcgcg acggtcttgc gccagccgga gtgtcgctgg aggtctccgg
     5401 cgcggcggac cagctcgacg cgactcgccg ttcgctcggc ctcaatttcg tcgtagcgac
     5461 gatcctcagc tacctgctgc tcgtcgcgat tttcacgcac tggggttggc cgctcatgat
     5521 cctgacgacc gttccgctcg gcctggcggg cggcgtgctc ggcctcgcgc tcgtgaacgc
     5581 gctgggcctg cgcctgccgt tcgacatgat cacgatgctg ggcttcctga tcctgctcgg
     5641 aacggtcgtc aacaacccga t
//
LOCUS       DASPFR010000104        13698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1008308, whole genome shotgun sequence.
ACCESSION   DASPFR010000104 DASPFR010000000
VERSION     DASPFR010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13698
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008308"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            966..6740
                     /locus_tag="VD737_02630"
     CDS             966..6740
                     /locus_tag="VD737_02630"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HXW09482.1"
                     /translation="MPHSLRSVGVACVLVCAWFTFPLAAGAGPGTGAPVATPVFTPTG
                     SVKSIRQVQARFATPMVVLGDPRLPDPFVIDCTGAGTGRWVDARTWVYDFGAELPGGL
                     GCRFTLQPGLEDLAGRAVRAARPFTFDTGGPRVTASLPYEGSESIDEEQVFLLKLDAA
                     ATPESVRAHARCRVEGLAESMPVTLVEGTEREAVLAQRRKLGYAYLRLLWSDGAVSEA
                     RERDPAFVAAERTLAMVRCQRRLPPGATVQLVWGRGIAAASGLATQQEQSLAFRVRPA
                     FTARVECARVNPDAGCLPFKPIDVRFTSPVPRALALGVRLLAAEGERPAQAIDANPAP
                     LLEGVRFDGPFAPSTTVKIVLPSQLVDDAGRLLGNAARFPLDVRVDEYPPLAKFAGTF
                     GVLEAREGGVLPVTLRNLEDAAAAGADAGIPGRRVRVGADPAVLAAWLRRVEKATESR
                     GDWRQDPESGRRQWRDLTGAASVFAAADGAESFAIAKPGGAREFEVVGIPLATPGLHV
                     VELQSRRLGQSLLGEDRPRYVATAALVTNLAVHFKWGRESSIAWVTALDTGRPVAEAA
                     VTVANYCDGKVRWTGRTDRDGIARIATTLGQPGASTWCGDAQGAPLLVLATLADDFSF
                     ALSSWSSGIEPWDFGMPVEYAETADLYHTVFDRPLFRPGETVSMKHYLRRHQAGGMGI
                     EPGLPAERKVVVQHSGSDQRYETVARFDAAGIAGQQWTLPGGARTGDYDVYVADARGQ
                     LRDSGGFKVEDFRLPTMRAEVSGPTGPLVLPTVVPLDLHVAYLSGGGASSLPVTLRTY
                     VEPRLVQLDGYDDYVFGGLPVQEGIETLGSGGDDADEAGGTGAGETRVQVRPLTLDAS
                     GAARIEVPGVPDLERSSVLTAELEYPDANGELLTATRRVRLDTASLHVGLRREGWVGT
                     SEKLRFRVLAVDLDGRPIRRQAVEARLYQVAHYSYRKRLVGGFYAYETSRETRKLDAS
                     CAGRTDARGLLACEVAPGVSGEIVVRAEARDPGGRLAGATASMWVVGADDWWFGGTAG
                     DRMDVLPERPAYETGDKARLQVRMPFRKATALVTVEREGVLDAFVKTLSGREPVVSLP
                     ITAAHAPNVFVSVLAVRGRVTRPEGPAGVAKAPVVTALVDLNKPAFRLGMAALRVGWK
                     PHRLDVSVRPDRGTYRVRDTAKVQVEVRRADGAPLPDGAEVALAAVDEALLELAPNPS
                     WQLLEAMMQRRGLQVWTSTAQLQVVGKRHYGRKAVPHGGGGGRDRARRSFDTLLLWQA
                     RVALDATGRATVDVPLNDSLSSFRIVAVASAGEQTFGTGSATIASSQELILTSGLPAL
                     VRDGDRFLATYTVRNTTQRKLRATVTAQVQPATAGALPSREVVLAPGEARDVAWTVQV
                     PPGTGTLAWNVAADAADGEARDRLQVSQSVVAAVPVRVYQATLAQVSGDFAIPVQRPS
                     DAVPGRGGVEVALRAKLADNLDGVREYMNAYPYQCLEQNLSRAIALEDRTQWDAWMNR
                     LPAYLDGAGLLKYFVSEAHPGEDVLTAYVLTIASVAGWDIPDAPRQRMLEGLAQFVTG
                     RIQRDSALPTADLAVRKLAAIAALARHGAAEASMLDTLAIDPDGWPTSAVLDWLDVLH
                     RLPDVRSKATRVAAAEKTLRSRLTLHGTQLMLSTERRDALWWLMVSADSNAARTVLAA
                     LERPQWRDDVPRLVQGLLARQQRGHWSTTTANAWAVTALRRYSDQFEKTPVGGSTRVT
                     LGGQGGEVQWAHDEPREVRLQWPPGPAELDIVHGGAGRPWALVQATAAIPLRAPLQSG
                     FTITRSVTAIETQRPGRWTRGDVARVRLDVDAQQDATWVVVDDPIPAGATILGTGLGG
                     QSQRLTEGERRSGAAWPAFEERRSDAFRAYYRFVPQGRWTVEYTVRLDNPGTFLLPPT
                     RVEAMYAPEIHAERPNAAVTIEPR"
     gene            6737..8887
                     /gene="pbpC"
                     /locus_tag="VD737_02635"
     CDS             6737..8887
                     /gene="pbpC"
                     /locus_tag="VD737_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HXW09483.1"
                     /translation="MSFGAHIGSRGLRAVLAATVLTSLAALAWLCVPAEVPAYEQVRA
                     RWQPSDAWLLDRRGEVIARQRMDFEVRRLEWTALNDISPALVEAIVEGEDRRFWQHGG
                     VDWRGVAGALRDTVLRDRRRGASTITMQVARLLDDADAARPGERGVWHKVQQVRRARG
                     LEARWTKPQILEAYLNLLGFRGELQGVGAAAEVLAGKLPRGIDRAESAVLAALLPAPG
                     ATLERTVTRACVRLRVDTQGGDCAAIRTAATAMLARRRLPLPAPELAPHVARALLREP
                     GERVTTTLDARVQRLAGQSLARHLASVGGRNVRDGAVLVVDTATGDVLAYVGSARETS
                     RAAAVDGVRALRQAGSTLKPFLYGLAVERGYLTAASLLDDSPLNLDTANGLYIPQNYD
                     RGFKGPVSVRTALGNSLNVPAVRALVLTGVDDFRERLHDLGYAAISREGDYYGYSLAL
                     GSAEVSLWQQAQAYRALARGGIVAPLRLRPDRPDASVPGRPVMSAEASFIVANVLADR
                     AARALTFGSDSVLSTSHWSAVKTGTSKDMRDNWCVGFSPRYLVAVWVGNFEGDSMHDV
                     TGVSGAAPVWAELMEALHRDSIVAPPEAPRGVSRVHVEYSPAHEAARDEWFVTGTEGA
                     VVEAVGSAQALARIASPAHATVIALDPDIPARAQRVPLRARGHLEGLVFELDGMSLGE
                     AQRPLLWSPAPGVHRLALVDHAGRTVDHVRFTVH"
     gene            complement(8891..10066)
                     /locus_tag="VD737_02640"
     CDS             complement(8891..10066)
                     /locus_tag="VD737_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008002122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXW09484.1"
                     /translation="MPAPDLNPLDLYDVRAAISDEERMVQDTVGRFVDDAVLPIIREH
                     FENHTFPRELVAELANLGLLGSSLEGYGCAGMNAVSYGLICQELERGDSGVRSFVSVQ
                     SSLCMYPIHAYGSEEQKQRYLPPMARGEVIGCFGLTEPHGGSDPGNMKTHAKRRGGDW
                     VLNGAKMWITNSPIADLFVIWARTEEGVRGFLVEKGTRGLAAPEIEKKFSLRASATGA
                     IFLEDVVVPASAMLPGSTCGLKAPLSCLTQARYGITWGVIGAAQACLREALEYSKTRI
                     LFNRPLAATQAIQIRLAEMSRKITTSQLLSLQLGRLKDAGRMTPSHVSLAKWNNCRAA
                     IDVARDARDILGGAGISAEYVPIRHALNLESVITYEGTETIHQLTIGRELTGINAFG"
     gene            complement(10103..10750)
                     /locus_tag="VD737_02645"
     CDS             complement(10103..10750)
                     /locus_tag="VD737_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09485.1"
                     /translation="MPARAHCSTRPARPPTARLWIVALALLSITACEGRRREPAERAI
                     AEIEAALADAGTAPAKYIPGQLADVRGDLDELEREYAAGRYRAVLDRAPRTLAAARAL
                     APSAAARAAELDRTLRTEWATLSQAVPIELSAAAERFDRLAARQKLPSGVSHDDVRSA
                     KGRLRDALALWDRARLEADAGRLPEAVTLAHQVREMGRSVTALSSPPGAAAAPVE"
     gene            10826..12136
                     /locus_tag="VD737_02650"
     CDS             10826..12136
                     /locus_tag="VD737_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HXW09486.1"
                     /translation="MKIPEILLVETYQKPGALASVLEVIAGEKLMIEHLQSLRREQGR
                     TLWEITVEIDEQAHPEFYGRINALPHARFVGKSDRVFNRHKGGKIHMEPSLQITTQQI
                     LRDIYTPGVARVCLAIRDDESRAALYTNRQNTVAVVTNGTAILGLGDIGPVAGLPVME
                     GKSALFAALVGITAYPILIDSRDSTRIVDTVAAIAPSFGAIQLEDIAAPECFEIEAEL
                     VRRLSIPVLHDDQHGTAVVALAGLINAVKRVGRTLDQSVVGQVGLGAAGLGIARLLRA
                     QGVRRLLGADLRADAIERLRQLGGEPASLQELMAQADIVVATTGVKGLIKPEWVRPGQ
                     IVFALSNPDAEIEPMAALERGAVFAADGKGINNVAAFPGLFKGALEAGAPRFTDGMLV
                     AAAHALARRAGPEELLPDALNLDTHRAVTAAVRAAGVEPVFSAL"
     gene            12207..12911
                     /locus_tag="VD737_02655"
     CDS             12207..12911
                     /locus_tag="VD737_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXW09487.1"
                     /translation="MSTLRINRSGIVALAAAGAVALSGCAAMQDVATSPEKENTRRGA
                     GYGAAAGAVIGLLTGGNDKFQAAMIGAAAGALAGGAVGYYMDKQEAKLREQMAGTGVD
                     VVRQGDNIVLDVPGGVTFAFDSADLNSQFYPVLDKVASTLTEYDKTVIEVAGHTDSVG
                     SDDYNQQLSERRANSVASYLSGHGVDRSRVVTIGAGEGHPVATNDTDAGRAQNRRVEI
                     TIVPVTQENVEKAKQG"
     gene            complement(13013..>13698)
                     /gene="rpoH"
                     /locus_tag="VD737_02660"
     CDS             complement(13013..>13698)
                     /gene="rpoH"
                     /locus_tag="VD737_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007939338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HXW09488.1"
                     /translation="VHIARGYAGYGLPVGDLVQEGNVGLMKAVRRFDPTVGVRLVSFA
                     VHWIRAEIHEYVLRNWRLVKIATTKAQRKLFFNLRKYKRNLGWLSAEETRAVARDLGV
                     SEREVTEMERRLTSRDVSYDPLPESDDDESANYSPAAYLPAPDADPAVAVERAEWDDD
                     VADRVAFAMTQLDDRSRTIMKARWMDEPKATLHDLADQFGVSAERIRQIEANAIKKLK
                     KLVADEQAA"
BASE COUNT         1990 a         4729 c         4936 g         2043 t
ORIGIN      
        1 attccgctgc catccatggt gatcacccga agactgccgg ctcgtaccgg ctcactgata
       61 ctaaccgtcg agttgtccag acggatggcc gttagaaagc cgttgtcggg tccattgcgg
      121 tcactctgcg tgtccgaaag catgcgtttc ccaaccacgc agtcgcccac acgaccgcga
      181 aagtttcagt gatcccggca gtggatgcct caggcgacgg atgagtggcg acggacatca
      241 cggcgacgta gcgcccgggc gcgactcggc cagtccacgt cgcgcgttgc gagatgggac
      301 cgtgattgag cgtacgaccc agtaaacgca tgctcccgcg agaagggctg cgacctggag
      361 gtcgaagcca atgaagtgga tcgtctctac caagctcatc gtcatgatgt gtccagccat
      421 gcaaaactca gggcttggat tagatgctgc gatgcagcat acgtcgctgc ggtgccgcac
      481 caaccgatac tggaggccag aaagaaaaag ccccgccaaa gcggggccgg gtcatctcga
      541 gcgcgtctct tctgcttgtt cgcgtttttc ttcttcggcg cacctaagaa ccagatttgt
      601 tgttcgatgc tccctgttct tcgccgtctt ccttccgatc gatcccctgg cggacaaccc
      661 tctcccgagc tggcttgccc gtccttcgcc cgtcggcggt tgttagctct catgggccaa
      721 tgaaggacgg gtgaagagga gcaagcctcg tgccaacggt cggtcatgcc agtcaaatca
      781 tgcggttacg tcgagcggat gcaggtattg cggcttctcg agcgtgtaaa gccttgattt
      841 cgtaaagaat gccgacaagc gtctgcgtga cgacgatgcg tgctcgaacg tcgcgtcacg
      901 ccgcgcggcc cggcgtgaca gcgtgcgcac ccgttcacac aatcgacgag aagcggatcc
      961 ccctcatgcc tcactcgtta cgttcggtcg gcgttgcgtg cgtactcgtc tgcgcatggt
     1021 tcacattccc gctcgccgcg ggcgccgggc cgggcaccgg cgcgccggtg gccacgcccg
     1081 tgttcacacc gacgggatcg gtcaagtcga tccgacaggt ccaggcgcgt ttcgcgacgc
     1141 cgatggtggt gctcggcgat ccgcggctgc cagacccgtt cgtgatcgac tgcaccggcg
     1201 cgggcaccgg tcgctgggtg gacgcgcgaa cgtgggtcta cgacttcggc gctgagctgc
     1261 ccggcgggtt agggtgcagg ttcacgttgc agccgggcct cgaggatctc gccggccgcg
     1321 ccgtccgcgc ggcgaggcca ttcacgttcg acacgggcgg tccgcgcgtg acggcttcgt
     1381 tgccgtacga aggctccgag tcgatcgacg aggagcaggt gttcctgctc aagctcgatg
     1441 cggcggcgac gcccgagtcg gtgcgtgccc acgcgcgctg ccgcgtcgaa gggctcgcgg
     1501 agtcgatgcc cgtgacgctc gtcgaaggca ccgagcgcga ggcggtgctc gcgcagcggc
     1561 gcaagctcgg ctacgcgtac ttgcggctgc tctggagcga cggtgccgtg tccgaagcgc
     1621 gcgagcgcga cccggctttc gtcgccgcgg aacggacgct cgccatggtg cgctgccagc
     1681 gccggctgcc accgggcgcg accgtccagc tggtctgggg ccgcggcatc gcggccgcga
     1741 gcgggctcgc gacgcaacag gagcagtcgc tcgcgttccg ggtgcggccg gccttcacgg
     1801 cacgcgtcga gtgcgcacgc gtgaacccgg acgcagggtg cctgccgttc aagccgatcg
     1861 acgtacggtt cacgtccccc gtgccgcgcg cgctcgcgct cggcgtgcgg ctcctggcgg
     1921 ccgagggcga gcgacccgcc caggcgatcg acgcgaaccc tgcgccactg ctcgaaggcg
     1981 tcaggttcga cgggccgttt gcgccctcca cgaccgtgaa gatcgtcctg ccgtcgcagc
     2041 tcgtcgacga cgccggtcga ttgctcggca acgccgcgcg ttttccgctc gacgtgcgtg
     2101 tggacgagta tccgccgctc gcgaagttcg ccggcacgtt cggcgtgctc gaggcgcgcg
     2161 agggcggggt gttgcccgtg acgctgcgca acctcgagga cgcggccgcg gcgggcgcgg
     2221 acgccggcat accgggccga cgcgtgcggg tcggcgccga ccccgccgtg ctcgccgcct
     2281 ggctgcgccg ggtcgagaaa gccacggaat cgcggggcga ttggcggcag gaccctgaga
     2341 gcgggcgccg gcaatggcgc gacctgaccg gcgcggcctc ggtcttcgcc gccgccgacg
     2401 gtgccgagtc gttcgcgatc gcgaagcccg gcggcgcgcg cgagttcgag gtcgtcggca
     2461 ttcccctcgc gacgccaggc ctgcacgtgg tcgagctgca gagccgccgg ctcggacaga
     2521 gcttgctcgg tgaggaccga ccgcgttacg tcgcgaccgc ggcgctcgtc acgaacctcg
     2581 cggtgcattt caagtggggc cgcgaatcct cgatcgcctg ggtgaccgcg ctcgacacgg
     2641 gccggccggt cgcggaggcc gccgtgaccg tcgccaacta ctgcgacggc aaggtccgct
     2701 ggaccgggcg cacggatcgc gacggcatcg cgcgcatcgc gacgacgctc ggccagccgg
     2761 gagcctccac ctggtgcggc gatgcgcagg gcgcgccgct gctcgtgctg gcgacgcttg
     2821 cggacgactt cagcttcgcg ctgtcgtcgt ggtcgagtgg catcgagccg tgggatttcg
     2881 gcatgcccgt ggagtacgcc gagacggccg acctgtacca cacggtgttc gaccggcccc
     2941 tgttccgccc tggcgagacg gtgtccatga agcactacct gcgtcgccac caggccggcg
     3001 ggatgggcat cgagcccggc ctgccggccg agcgcaaggt cgtcgtgcag cattcgggca
     3061 gcgaccagcg ctacgagact gtcgcgcgct tcgatgcagc gggcatcgcc ggccagcagt
     3121 ggacgctgcc tggcggggcg cgcaccgggg attacgacgt gtacgtggcc gatgcgcgcg
     3181 gacagttgcg cgacagcggt ggcttcaagg tcgaggattt ccggttgccg acgatgcgcg
     3241 ccgaggtcag cggtccgacc gggccgctgg tattgccgac cgtcgtgccg ctcgacctgc
     3301 acgtcgcgta cctgtccggc ggcggcgcct cgtcgctgcc ggtgaccctg cgcacgtacg
     3361 tcgaaccgcg cctcgtccag ttggacggtt acgacgacta cgtcttcggc ggcctgccgg
     3421 tgcaggaagg catcgagacg ctcggcagcg gcggcgacga cgccgacgag gccggcggca
     3481 cgggggcggg cgagacgcgc gtgcaggtcc ggccgctcac gctcgacgcg agcggtgcgg
     3541 cgcgcatcga agtgcccggc gtgcccgacc tcgaacgaag cagcgtgctg accgccgaac
     3601 tggagtaccc ggacgccaac ggcgaactgt tgacggcaac ccgccgcgtg cggctcgaca
     3661 ccgcgtcgct gcacgtgggg ctgcgtcgcg agggctgggt cggcaccagc gagaagctgc
     3721 gcttccgcgt gctggcagtc gacctggacg gccggccgat caggcgacaa gccgttgagg
     3781 ccaggctgta ccaggtggct cactactcgt accgcaagcg cctcgtcggc ggcttctacg
     3841 cgtacgagac cagtcgcgag acgcgcaagc tcgacgcgag ttgcgccggc cgcacggacg
     3901 cgcgcgggct gctcgcctgc gaagtggcgc ccggcgtctc gggcgaaatc gtcgtacgcg
     3961 cggaggcgcg cgaccccggc ggacggctcg ccggcgcgac cgcgtcgatg tgggtggtcg
     4021 gcgccgacga ctggtggttc gggggcaccg ccggcgaccg catggacgtg ctgccggaac
     4081 ggccggcgta cgagaccggc gacaaggcgc ggctgcaagt ccgcatgccg ttccgcaagg
     4141 cgacggcgct ggtcacggtc gagcgggagg gcgtgctcga cgcattcgtg aagacgttga
     4201 gcggacgcga gccagtggtg agcctgccga tcacggcggc gcacgccccc aacgtcttcg
     4261 tctccgtgct cgcggtgcgg gggcgcgtga ccagaccgga aggcccggct ggcgtggcga
     4321 aggcgcccgt cgtgacggcg ctcgtcgacc tcaacaagcc cgcgttccgg ctcggcatgg
     4381 cggcgcttcg ggtcggctgg aaaccgcacc ggctcgacgt gtccgtgcgg ccggaccgcg
     4441 gcacgtaccg ggtccgggac acggcgaagg tgcaggtcga ggtgcgtcgc gccgacggag
     4501 cgccgctgcc cgatggcgcc gaagtcgcgc tcgcggccgt cgacgaggcg ttgctcgaac
     4561 tggcgccgaa tccgagctgg cagctgctcg aggcgatgat gcagcgacgc ggcctccagg
     4621 tctggacctc caccgcacag ttgcaggtgg tgggcaagcg acactacggt cgcaaggccg
     4681 tgccgcacgg cggcggtggc gggcgcgatc gcgcgcgacg ctcgttcgac accctgctgc
     4741 tgtggcaggc gcgcgtggcg ctggacgcga cgggccgcgc gacggtggac gtgccgctca
     4801 acgactcgct gagctcgttc cgcatcgtcg cggtcgcgag cgccggcgag cagaccttcg
     4861 gcaccggcag cgcgacgatc gcctcgtcgc aggagctgat cctgacgtcg ggattgcctg
     4921 cgctcgtgcg cgacggcgac cgcttcctcg cgacgtacac ggtgcgcaac acgacgcagc
     4981 gcaaactgcg cgcgacggtc accgcgcaag tgcagccggc cacggccggc gcgttgccat
     5041 cgcgcgaggt cgtgctcgcg cccggcgagg cgcgcgacgt cgcctggacg gtgcaggtgc
     5101 cgcccgggac cgggacgctc gcctggaacg tcgcggcaga cgcggcggac ggcgaggccc
     5161 gcgaccggct gcaggtcagc cagtcggtcg tcgcggccgt gccggtgcgg gtctaccagg
     5221 cgacgctggc ccaagtgtcg ggcgacttcg cgatccccgt gcagcgaccc tctgatgccg
     5281 tcccgggacg cggcggagtc gaggtcgcct tgcgcgcgaa actggcggac aacctcgacg
     5341 gcgttcgcga atacatgaat gcgtatccgt accagtgcct cgagcagaac ctgtcccgcg
     5401 cgatcgcgct cgaggaccgc acgcagtggg acgcctggat gaaccggctg ccggcctacc
     5461 tcgacggcgc cggtctactg aaatacttcg tgtcggaggc acacccgggc gaggacgtcc
     5521 tgaccgcgta cgtcctgacg atcgcgagcg tcgccggatg ggacatcccc gatgcgccgc
     5581 gccagcgcat gctggaaggt ctcgcccagt tcgtcaccgg ccgcatccag cgcgactcgg
     5641 cgctgccgac cgccgatctc gcggtgcgca agctcgccgc catcgctgca ctggcgcgcc
     5701 acggcgcggc cgaggcatcg atgctcgaca cgctcgccat cgatccggac ggctggccca
     5761 cctcggcggt gctggactgg ctcgacgtgc tccatcgatt gccggacgtg cggtcgaagg
     5821 cgacgcgcgt cgcggcagcc gagaagaccc tgcgctcgcg gctgacgctg cacggcacgc
     5881 aactcatgct gtccaccgag cgtcgggacg cgctctggtg gctgatggtc tccgcggaca
     5941 gcaacgcggc gcgcaccgtg ctcgccgcat tggaacgccc gcagtggcgc gacgacgtac
     6001 cgcggctggt gcaggggctg ctggcgcgcc agcagcgcgg gcactggagc accacgacgg
     6061 caaacgcgtg ggcggtgacg gccctgcggc gctactcgga ccagttcgag aagaccccgg
     6121 tcggcgggtc cacccgcgtc acgctcggag gccagggcgg cgaggtgcag tgggcccacg
     6181 acgagccgcg cgaagtccgg ctgcaatggc ctccggggcc tgccgaactc gacatcgtgc
     6241 acgggggcgc cggccgaccg tgggcactcg tgcaggccac cgccgcgatt ccgctccgcg
     6301 cgccgctgca gagcggcttt acgatcacgc gctccgtgac cgcgatcgag acgcaacggc
     6361 ccggccgctg gacgcgcggt gacgtcgcgc gcgtccggct cgacgtcgac gcacagcagg
     6421 acgcgacctg ggtcgtcgtc gacgatccca tccccgccgg tgccacgatc ctcgggaccg
     6481 ggctcggcgg tcagtcgcaa cgcctgaccg aaggcgagcg tcgctcaggc gctgcctggc
     6541 ccgccttcga ggagcgccgc tccgacgcgt tccgcgcgta ctaccggttc gtgccgcagg
     6601 gccgctggac ggtcgagtac accgtgcggc tcgacaatcc cggcacgttc ctgctgccgc
     6661 cgacgcgcgt cgaagcaatg tacgcgcccg aaatccacgc cgagcgtccg aacgccgccg
     6721 tgacgatcga gccgcggtga gcttcggcgc gcacatcggc tcacgcggcc tgcgcgcggt
     6781 cctcgccgcg acggtgctca cgtcgctggc ggcgctcgcg tggctgtgcg tcccggccga
     6841 ggttcccgcg tacgagcagg tacgcgcacg ctggcaaccc tcggacgcct ggctgctcga
     6901 ccggcgcggc gaggtcatcg cgcggcaacg catggacttc gaggtgcggc gcctggagtg
     6961 gaccgcgctg aacgacattt caccggcgct ggtcgaggcg atcgtggagg gagaggaccg
     7021 ccggttctgg cagcacggcg gtgtcgactg gcgtggcgtg gcgggcgcgc tccgcgacac
     7081 ggtcctgcgc gatcgtcggc ggggcgcgag cacgatcacg atgcaggtcg cacgcctgct
     7141 ggacgatgcg gacgcggccc gcccgggcga gcggggggtc tggcacaagg ttcagcaggt
     7201 gcgccgggcg cgcgggctcg aggcgcgctg gaccaagccg cagatcctcg aggcttacct
     7261 caacctgctg gggttccgcg gcgaactgca gggtgtcggt gccgccgcgg aagtgctcgc
     7321 cggcaagctg ccgcgcggca tcgaccgcgc cgaaagcgcc gtgctcgcgg cgctgttgcc
     7381 cgcgccgggc gcgaccctcg agcgcaccgt gacgcgcgcg tgtgtgcgcc tgcgcgtcga
     7441 cacccagggc ggcgactgcg ccgcgatacg gactgccgcg accgcgatgc tcgcgcggcg
     7501 tcgcctgccg ctgcccgcac ccgagctcgc gcctcacgtc gcgcgtgcgc tgctgcgcga
     7561 accgggcgag cgcgtgacga cgacgctcga cgcgcgcgtg caacgcctcg ccggccagtc
     7621 gctcgcgcgg cacctcgcga gcgtcggcgg gcgcaacgtc cgcgacggcg cggtgctggt
     7681 cgtcgacacg gcgacgggcg acgtcctcgc gtacgtggga tcggcgcgcg agacttcacg
     7741 tgccgccgcc gtcgacggcg ttcgcgcgct gcgccaggcc ggctccacgc tgaagccgtt
     7801 cctgtacggt ctcgcggtcg agcgcggtta cctgacggcg gcgtcgctgc tcgacgattc
     7861 accgctcaac ctcgacaccg cgaacggcct ctacatcccg cagaactacg accgtggctt
     7921 caagggtccc gtcagcgtgc gcaccgcgct cggcaattcg ctgaacgtac cggccgtccg
     7981 cgcgctcgtg ctgaccggcg tcgacgactt tcgcgagcgc ctccacgacc tcggctacgc
     8041 ggcgatctcg cgcgaaggcg actactacgg ctattcgctg gcgctcggct cggccgaggt
     8101 gagcttgtgg caacaggcgc aggcctatcg cgcgctggcc cgcggtggga tcgtggcgcc
     8161 gctcaggctg cgcccggacc ggcccgatgc atcggtgccg ggtcggccgg tcatgtcggc
     8221 ggaggccagt ttcatcgtcg ccaacgtgct cgccgaccgg gccgcacgcg cgctgacgtt
     8281 cggctccgac agcgtgctca gcacgtctca ctggagcgcg gtcaagaccg gcaccagcaa
     8341 ggacatgcgc gacaactggt gcgtcgggtt ctcgccccgc tacctcgtcg cggtgtgggt
     8401 cggcaacttc gaaggcgact cgatgcacga cgtcacgggc gtgagtggcg cggcgccggt
     8461 ctgggccgag ctgatggagg cgctgcatcg cgactcgatc gtcgcgccgc cggaagcgcc
     8521 gcgcggcgtg tcgcgtgtgc acgtcgagta ctcgccggca cacgaggccg cgcgcgacga
     8581 gtggttcgtc actggaaccg agggcgcggt ggtcgaggcg gtcgggtccg cacaggcgct
     8641 cgcccgcatc gcgagcccgg cgcacgcgac cgtgatcgcg ctggacccgg acatcccggc
     8701 gcgtgcgcag cgtgtgccgc tgcgtgcgcg gggtcacctg gagggactcg tcttcgagct
     8761 cgatggaatg tcgctgggcg aggcgcagcg accgctgctg tggtcaccgg cgccgggcgt
     8821 gcaccggctg gcgctcgtcg accacgcggg ccggaccgtg gatcacgtcc ggttcacggt
     8881 ccattgatcc tcagccgaac gcgttgatcc cggtcagctc acgaccgatc gtgagctggt
     8941 ggatggtctc ggtgccttcg taggtgatca cgctctcgag attgagcgcg tggcggatcg
     9001 gcacgtactc ggcgctgatg ccggcgccgc caaggatgtc gcgcgcgtcc cgcgccacgt
     9061 cgatcgcggc ccggcagttg ttccacttgg cgagcgaaac gtgcgacggc gtcatgcgtc
     9121 ccgcgtcctt gagccgcccg agctgcagcg agagcagctg cgacgtcgtg atcttgcgcg
     9181 acatctccgc caggcggatc tggatcgcct gcgtcgccgc gagcggccgg ttgaacagga
     9241 tgcgcgtctt tgaatactcg agcgcctcgc gcaggcacgc ctgggcggca ccgatcacgc
     9301 cccacgtgat gccgtagcgc gcctgcgtca ggcaggagag cggcgccttg aggccgcagg
     9361 tgctgccggg cagcatcgcg gacgccggca ccacgacgtc ctcgaggaag atcgcgccgg
     9421 tcgcggaggc ccgcagcgaa aacttcttct cgatctccgg cgcggcgagg ccgcgcgtgc
     9481 ctttctcgac caggaatccg cgcacgcctt cctcggtccg ggcccagatc acgaacaggt
     9541 cggcgatcgg cgagttcgtg atccacatct tggcgccgtt cagcacccag tcgccgccac
     9601 ggcgtttcgc gtgcgtcttc atgttgcccg gatcggaccc accgtgcggc tccgtgaggc
     9661 cgaaacagcc gatcacttca ccgcgagcca tcggcggcaa gtagcgctgc ttctgctcct
     9721 cgctgccgta ggcgtggatc ggatacatgc agagcgacga ctgtaccgag acgaagctgc
     9781 gcaccccgga atcgccgcgc tcgagttcct ggcagatcag cccgtagctc acggcgttca
     9841 tgcctgcaca gccgtaccct tccagcgagc tgccgagcag gccgaggttg gcgagctcgg
     9901 cgacgagctc gcgcggaaac gtgtggttct cgaaatgctc gcggatgatc ggcaggaccg
     9961 cgtcgtcgac gaagcgaccg accgtgtcct gcaccatgcg ttcctcgtcg ctgatcgcgg
    10021 cgcgtacgtc gtacaggtcg agggggttca ggtctggggc gggcaaggtc ggctcccggt
    10081 cggaggcgga gaacgcgaca ttttactcga ccggggcggc ggctgcgccc ggcggcgacg
    10141 agagggcggt caccgagcgg cccatctcgc ggacctggtg ggcgagcgtc actgcttccg
    10201 gcaggcggcc ggcgtctgct tcgaggcgcg cacggtccca cagcgcgagt gcgtcgcgaa
    10261 gtcgcccctt ggcgcttcgc acgtcgtcgt gcgacacgcc ggacggcagc ttctgacgcg
    10321 ccgcgagccg atcgaaccgc tcggccgccg cgctgagctc gatcggcacg gcctggctca
    10381 gggtcgccca ctcggtgcgc agcgtgcgat cgagctcggc cgcgcgcgcc gcggccgacg
    10441 gtgccagtgc gcgtgcggcc gcgagcgtcc ggggcgcgcg atcgagcacg gcgcggtagc
    10501 gacccgcggc gtactcgcgt tcgagctcgt ccaggtctcc gcgcacgtcc gcgagctggc
    10561 cgggaatgta cttcgcgggc gccgtgcccg catcggcgag cgccgcctcg atctcggcga
    10621 tcgcgcgttc ggccggttca cgccttcgac cctcgcaggc cgtgatcgag agcagggcca
    10681 gcgcgacgat ccacagccgc gccgtcggcg gacgggcagg acgcgtggag cagtgggcgc
    10741 gggctggcat gaggcggcgg gtccatgggc ggcgggcgcg gacgggctgc tatcgtatgc
    10801 ggcccggccc gggagatgtg tgcgcgtgaa gattccagag atcctgctcg tcgagacgta
    10861 ccagaagccc ggcgcgctcg cgagcgtgct cgaggtgatc gccggcgaga agctcatgat
    10921 cgagcacctg cagtcgctgc gacgcgagca gggcaggacg ctatgggaaa tcaccgtcga
    10981 aatcgacgag caggcgcacc ccgagttcta cggtcgcatc aacgcgctgc cgcacgcgcg
    11041 cttcgtcggc aagtccgacc gcgtcttcaa ccgccacaag ggcggcaaga tccacatgga
    11101 gccgtcgctg cagatcacga cccagcagat cctgcgcgac atctacacgc cgggcgtggc
    11161 acgcgtctgc ctcgcgatcc gtgacgacga gtcccgggcg gcgctctaca ccaatcgcca
    11221 gaacaccgtc gcggtcgtca cgaatggcac cgcgatcctg gggctcggcg acatcggccc
    11281 ggtggccggc ctgccggtca tggaaggcaa gtccgcgctg ttcgcggcgc tggtcggcat
    11341 cacggcatac ccgatcctga tcgactccag ggactccacg cgcatcgtcg acacggtcgc
    11401 ggcgatcgcg ccgtcgttcg gcgccatcca gctcgaggac atcgccgccc ccgagtgctt
    11461 cgagatcgag gccgagctcg tccgccggct gtcgattccg gtgctgcacg acgaccagca
    11521 cggcacggcg gtggtcgcac tggccgggct gatcaacgcg gtcaagcgcg tcggccgcac
    11581 cctggaccag tcggtcgtgg gccaggtcgg cctcggcgcg gcgggcctcg gaatcgcgcg
    11641 gctgcttcgc gcgcagggcg tgcggcgcct gctcggcgcg gatctgcgcg ccgacgcgat
    11701 cgagcggctg cgccagctcg gcggcgagcc ggcgtcgctg caggagctga tggcgcaggc
    11761 cgacatcgtc gtcgcgacga ccggcgtcaa ggggctgatc aagcccgagt gggtgcgccc
    11821 gggccagatc gtgttcgcgt tgtcgaatcc cgacgcggag atcgaaccca tggcggcgct
    11881 cgagcgcggg gccgtcttcg ccgctgacgg taagggcatc aacaacgttg cggcatttcc
    11941 cggcctgttc aagggtgcac tggaggctgg ggcgccgcgg ttcaccgatg gcatgctggt
    12001 cgcagccgcc cacgccctgg cacgacgggc cgggcccgag gaacttctgc ccgatgcgct
    12061 gaatctggat acgcaccggg ccgtgacggc ggccgtgcgg gcggccggcg tggagccggt
    12121 gttcagcgcg ctttgacgat agtgtggccc gtcaacgggt gacgcgatcg gacgttaagt
    12181 tttcattccc agcaggagtt ccgagcatga gcacccttcg aatcaacagg tcgggaatcg
    12241 tcgcactggc ggccgcgggc gccgtggcgc tgagcggctg cgccgccatg caggacgtgg
    12301 cgaccagtcc cgagaaggaa aacacccgtc gcggcgccgg gtacggcgcg gcggccggcg
    12361 cggtcatcgg cctgctgacg ggcggcaacg acaagttcca ggccgcgatg atcggcgcgg
    12421 cggccggtgc gctggcgggc ggcgcggtcg ggtactacat ggacaagcag gaagcgaagc
    12481 tccgcgaaca gatggccggt acgggcgtcg acgtcgtccg ccagggcgac aacatcgtgc
    12541 tcgacgtgcc cggcggcgtc acgttcgcgt tcgacagcgc tgacctcaac tcgcagttct
    12601 acccggtgct cgacaaggtg gcctcgacgc tcaccgagta cgacaagacc gtgatcgagg
    12661 tcgccggcca caccgacagc gtcggcagcg acgactacaa ccagcagctc tccgagcgtc
    12721 gcgccaacag cgtggcctcg tacctgtcgg gccacggcgt cgaccggtca cgtgttgtga
    12781 cgatcggcgc gggcgagggt caccccgtcg cgaccaacga cactgacgcc ggccgcgcgc
    12841 agaaccgccg cgtcgagatc acgatcgtcc ccgtgacgca ggagaacgtg gagaaggcga
    12901 agcagggatg aggaggaagt gggcagtgac cagtggttag tggtcagtgc cagaaagcaa
    12961 agaggccggc gaaattcgcc ggcctctgtc atttcaaact agccactagc cactacgccg
    13021 cctgctcgtc cgcgacgagc ttcttgagct tcttgatcgc gttcgcctcg atctggcgga
    13081 tgcgctccgc cgagacgccg aactggtcgg cgaggtcgtg cagcgtggcc ttgggctcgt
    13141 ccatccagcg cgccttcatg atcgtgcggc tgcggtcgtc gagctgagtc atcgcgaacg
    13201 cgacgcggtc ggccacgtcg tcgtcccact cggcgcgctc cacggccacc gccgggtcgg
    13261 cgtccggtgc cggcaggtac gcggccggcg aatagttcgc ggactcgtcg tcgtcggact
    13321 ccggcaacgg atcgtacgag acgtcgcggg aggtgagccg ccgctccatc tcggtgacct
    13381 cgcgctcgct cacgcccagg tcgcgcgcga cggcgcgggt ctcttcggcg ctgagccaac
    13441 cgaggttgcg cttgtacttg cgcaggttga agaacagctt gcgctgcgct ttcgtcgtgg
    13501 cgatcttcac gagccgccag ttgcgcagca cgtactcgtg aatctccgcg cggatccagt
    13561 gcacggcgaa cgagacgagt cgcacgccga cggtcgggtc gaagcgacgc acggccttca
    13621 tgaggccgac gttgccttcc tgcacgaggt cgccgacggg cagcccgtaa cccgcgtacc
    13681 cgcgcgcgat gtgcacga
//
LOCUS       DASPFR010000105         6672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1497101, whole genome shotgun sequence.
ACCESSION   DASPFR010000105 DASPFR010000000
VERSION     DASPFR010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497101"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VD737_02665"
     CDS             <1..313
                     /locus_tag="VD737_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09489.1"
                     /translation="GRTATAQACLAEVLAITAKLPSGYQRDLQLIKAPLFRSIDVCLE
                     SLDIMAAAIPQIRFRPERIRLDADIHAAGEANALVAREGIPFREAYRRVGARLEDDPA
                     G"
     gene            452..1810
                     /locus_tag="VD737_02670"
     CDS             452..1810
                     /locus_tag="VD737_02670"
                     /inference="COORDINATES: protein motif:HMM:NF018716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HXW09490.1"
                     /translation="MHTTMNRRRHRAAAWLVTSLFCATATGAAPDHSRLGQDLTPVGA
                     ERAGNADGSIPAWEGGLTQAPAGWTPQQGYVDPFPDDKPLFTITAANAAQYESRLTKG
                     QLALLKKHPQGFRMNVYATRRTVGYPKAVTDKVVAQAGKVSLQGFGLKDLGGSTTPFP
                     IPQNGLEAIWNHLVRYLGGGIVRAGHSFPVRPNGDYYKIGFRAQRIYAQNVEGAEDNR
                     LFYALGYFTEPATLRGTIFLVHEPIDQVAEQRSAWIYNAGARRVRRAPDLAYDGINDG
                     SEGMLTTDQVDAYNGAPDRYEWQLLGKREVYVPYNTYRLSDKSLRYKDIIRPGSINPD
                     LVRYELHRVWVVEGTLKPGQRHIYGKRTLYLDEDSWSVLAEDAYDTRGGLWRASMHGL
                     VQAYDASVPFYRFGIYHDLNSGGYLVGGLDNEMKEPIQFNAKARITEFQPDALRRLGG
                     AL"
     gene            complement(1899..2504)
                     /locus_tag="VD737_02675"
     CDS             complement(1899..2504)
                     /locus_tag="VD737_02675"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXW09491.1"
                     /translation="MSSVDVPGSRADAQRDRILCAALKCFIEHGFHAASMANIAEVAQ
                     MSPGLIYRYFDSKDAIVLAIVQRQLAEKQLAIRQLQSSEQIIDGLTQAFEQWGRRGAN
                     TMSVALQLEMSAEATRNPQIAEALRASDAQTRGQFEEWLRRPSAEGGAGVASGDVRAL
                     ALAVRVVVDGLTLRAAREPDLDADEIRAALELLVQPLLRSG"
     gene            complement(2507..3946)
                     /locus_tag="VD737_02680"
     CDS             complement(2507..3946)
                     /locus_tag="VD737_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HXW09492.1"
                     /translation="MTHVRPLRAALATGLAALVSACGVLEPRVPEARPGVPEQWPLPP
                     TTEGATPASPSSTAVPAVQAVADVGWRNFFVDPKLEELVARSLANNRDFRIAVLNVER
                     ARELYRIQRADRVPSVGAVGTMVRQGGDDRPVTETYSADLAVTGFELDLFGRVRSLSQ
                     SALQSYFAQEQARRSVQLSLIAEVANVYLTLAADQDQVRLAQDTLHTRAEAFRITQKR
                     HEFGAVSALDVYQSRTQVESARADLARYEGQVAQDLNALTLLVGGTVEPELRPAGFDP
                     NVTGLDALPPGLPSDVLLRRPDVLAAEHRLLAANANIGAARAAFFPSITLTGAVGVAS
                     SELSGLFDGGTGTWSFVPRINLPIFEGGRLRANLGAARADRDIALADYEGAIQRGFRE
                     VADALALTSTLAAQRLALEQLVEAAGQAEEIARVRYEAGRDSYLTRLEAQRTLYVAQQ
                     ALITTRLAEQGNRITLYKVLGGGWTEGTP"
     gene            complement(3943..>6672)
                     /locus_tag="VD737_02685"
     CDS             complement(3943..>6672)
                     /locus_tag="VD737_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXW09493.1"
                     /translation="IGFVSKDGSMNREDLADYVGANLRDPLSRVEGVGTVQLFGAPYA
                     MRIWLDPNKLDTYRLSVNDITTAVRAQNAQVTVGQLGGAPAVEGQQLNATISAQSRLQ
                     TPEQFRNIVVRSNPDGSSLRLGEVARVELGAESYEAITRFNGQPAVGMAISLATGANA
                     LATSNAVRERLDELRPFFPAGMETVIPFDTTPFVRAAIIEVVKTLAEAIVLVFLVIYL
                     FLQNFRATLIPTIAVPVVLLGTFGVLAALGFSINVLTMFAMVLAIGLLVDDAIVVVEN
                     VERLMREEGLSPLEATRKSMDQITGALVAIGLVLAAVFIPMAFLSGSVGVIYRQFTAT
                     IVSAMALSVLVAIVLTPALCATLLKPIRRGEHHEARGFFGWFNRAFDRGSHGYEGIVR
                     RVLSRTGRFLLVYLGLAAVMVLLFLRLPSAFLPDEDQGSLISIVQAPVGSTQQRTLQS
                     IARLEHHFMENEPAFKSIFSIVGFSFAGGGQNSGMAFVNLKPWDERDSDDLHVSAVAR
                     RAMGALSQIKDAMAFAFAPPPVPELGVATGFTFYLQDNAGNGHDALMAARNQLLGMTA
                     QSKVLANVRPNGQEDTPQFKLDIDTAKAAALGVAIADINATLSAAWGGSYIDDFIDRG
                     RVKRVYLQADAPFRMSPEDFRRWSVRNSEGLMVPVSSFATWHWEYGSPRLERYNGVSA
                     REIQGEAAPGVSTGQAMDEIERLVGELPPGFGIEWSGASYQERAAGAQTPLLYALSFA
                     IVFLCLAALYESWSIPTAVLMVAPLGILGATLASTLFGLQRDVYFQVAMLTTLGLTSK
                     NAILIIEFAKSNYEKGMDLLEGTMQAAKIRLRPILMTSLAFGFGVLPLALASGAGSGA
                     QRAIGTGVLGGMIVGTLLGVVFIPLFFVVVERWLVRKEPHPTTSQAAPAGGAA"
BASE COUNT         1087 a         2342 c         2273 g          970 t
ORIGIN      
        1 cgggcgcacg gccacggcgc aggcctgcct ggccgaggtg ctcgcgatca cggccaagct
       61 gccctcgggc taccagcgcg acctgcagct gatcaaggca ccgctgttcc gctcgatcga
      121 cgtgtgcctg gaatcgctgg acatcatggc ggcggcgatc ccgcagatcc ggttccgccc
      181 ggagcgaatc cgcctggacg ctgacatcca cgcggcggga gaggccaacg cgctggtcgc
      241 gcgcgagggc atcccgttcc gggaggcgta tcgccgcgtc ggggcccgcc tcgaggacga
      301 cccggccggc tgagccgatg acgcccgctc tgccggctgc gtcgccaggg tcgtcgccag
      361 cgcacacacc gtgggcctcg gcttcgtcgt cgcggccacg taggatggag ccccgcctct
      421 cgcacgagcg cgtcgaacga cgccgggacc catgcacacg acgatgaatc gccggcgcca
      481 tcgcgccgca gcctggctgg tgaccagcct cttctgcgcg accgcgaccg gcgccgcacc
      541 cgaccattcg cggctcggcc aggacctgac gcccgtcggc gccgagcgcg ccggcaatgc
      601 cgatggctcc atccccgcgt gggagggtgg cctgacgcag gcacccgccg ggtggactcc
      661 gcagcagggc tacgtcgatc cgtttccgga cgacaagccg ctgttcacga ttacggcagc
      721 gaacgcggcg cagtacgagt ccaggctgac caagggccag ctggcactgc tcaagaagca
      781 cccgcagggc ttccgcatga acgtctatgc gacgcggcgg accgtgggct acccgaaggc
      841 cgtcaccgac aaggtcgtgg cgcaggcggg caaggtgtcc ttgcagggct tcgggctcaa
      901 ggacctcggc ggctcgacca cgccgttccc gatcccgcag aacggcctgg aggcaatctg
      961 gaatcacctg gtgcgctacc tcggtggcgg catcgtgcgc gcgggacatt cgttcccagt
     1021 gcggccgaac ggcgattact acaagatcgg gtttcgcgcg cagcgcatct acgcgcagaa
     1081 cgtcgagggc gccgaggaca accggctctt ctacgcgctc ggctacttca ccgagccggc
     1141 gacgctgcgc ggcacgatct tcctggtcca cgaaccgatc gaccaggtcg cggaacagcg
     1201 ctccgcatgg atctacaacg ccggcgcgcg ccgcgtgcgc cgcgcccccg acctcgcgta
     1261 cgacggcatc aacgacggct ccgagggcat gctcacgacc gaccaggtcg acgcctacaa
     1321 cggcgcgccc gaccgctacg aatggcagct gctcggcaag cgtgaggtct acgtgccgta
     1381 caacacgtac aggctttcgg acaagtcgct caggtacaag gacatcatcc ggcccggctc
     1441 gatcaatccc gacctggtgc gctacgagct gcaccgcgtc tgggtggtgg aaggcacgct
     1501 caagcccggg cagcgccaca tctacggcaa gcgcacgtta tacctggacg aggacagctg
     1561 gtcggtgctc gccgaggacg cctacgacac gcgcggcggg ctgtggcgcg cgtcgatgca
     1621 cgggctcgtg caggcctacg acgcgagcgt gccgttctac cgtttcggca tctaccacga
     1681 cctgaacagc ggcggttatc tcgtcggcgg cctcgacaac gagatgaagg aaccgatcca
     1741 gttcaacgcc aaggcccgca tcaccgagtt ccagccggac gcgctgcggc ggctgggcgg
     1801 cgcgctgtga ggaagtggtc agtggctagt ggttagtggt tagtggaaga cgcaaccttc
     1861 gaggatcgct gtcttcacta gccactagcc actgaccact agccacttct caacagcggc
     1921 tgcaccagca gctcgagcgc cgcccggatc tcgtcggcgt cgaggtcggg ctcgcgggcc
     1981 gcccgcagcg tgaggccgtc gaccacgacg cggacggcga gcgcgagcgc gcgaacgtca
     2041 ccggacgcca cgccggcgcc accctcggcg gacggccgtc gcagccactc ctcgaactgg
     2101 ccgcgtgtct gcgcgtcgga ggcgcgcagc gcctcggcga tctgcgggtt gcgcgtggcc
     2161 tcggcgctca tctcgagctg cagcgcgaca ctcatggtgt tggcgccgcg acggccccat
     2221 tgctcgaacg cctgcgtgag cccgtcgatg atctgctcgg acgactgcag ctggcggatc
     2281 gccagctgct tctcggcgag ctggcgctgc acgatcgcga gcacgatggc gtccttgctg
     2341 tcgaagtagc ggtagatcag cccagggctc atctgcgcga cttcggcgat gttcgccatg
     2401 ctggccgcgt ggaacccgtg ctcgatgaag cacttgagcg cggcgcagag gatgcggtcg
     2461 cgctgcgcgt cggcgcgcga accggggacg tccacggacg acatgctcac ggcgtgccct
     2521 ccgtccagcc accgccgagc accttgtaga gcgtgatccg gttgccctgc tccgcgagcc
     2581 gcgtcgtgat cagcgcctgt tgcgcgacgt agagcgtgcg ttgcgcctcg agccgcgtga
     2641 ggtagctgtc gcgcccggcc tcgtagcgca cgcgcgcgat ctcctcggcc tggccggccg
     2701 cctcgacgag ctgctcgagc gccaggcgtt gcgccgcgag cgtcgaggtg agcgcgagcg
     2761 cgtccgcgac ctcgcggaaa ccgcgctgga tcgcaccctc gtagtcggcg agcgcgatat
     2821 ctcgatcggc cctcgctgcg ccgaggttgg cgcggagccg cccgccctcg aagatcggca
     2881 ggttgatccg cggcacgaag ctccacgtgc cggtgccgcc gtcgaacagg ccggagagct
     2941 cgctgctcgc gacgcccacc gcgccagtaa gcgtaatcga cgggaagaac gcggcgcggg
     3001 ccgcgccgat gttcgcgttc gcggcaagca gccgatgctc ggcggcgagc acgtccggcc
     3061 gccgcagcag cacgtcggag ggcagccccg gcggcagtgc gtcgagtccc gtcacgttgg
     3121 gatcgaagcc cgccggccga agctccggct ccacggtgcc gccgaccagc agcgtcagcg
     3181 cgttgaggtc ctgcgccacc tgcccctcgt aacgcgcgag gtccgctcgc gcggactcga
     3241 cctgcgtgcg cgactggtag acgtcgagcg cggacaccgc gccgaactcg tgccgcttct
     3301 gcgtgatgcg gaaagcctcc gcgcgcgtgt gcagcgtatc ttgcgcgagc cgcacctggt
     3361 cctggtccgc ggccagcgtc aggtacacgt tcgccacctc cgcgatcagg ctgagctgga
     3421 cgctgcgacg cgcctgctcc tgggcgaagt agctctgcag ggccgactgg ctcaggctgc
     3481 gcacgcggcc gaacaggtcg agttcaaagc cggtgaccgc caggtccgcg ctgtacgtct
     3541 ccgtcaccgg ccggtcgtcg ccgccctggc gcaccatcgt gccgacggcg ccgaccgacg
     3601 gcacacgatc ggcgcgctgg atccggtaga gctcgcgcgc acgctcgacg ttgagcacgg
     3661 cgatgcggaa atcgcggttg ttggcgagcg agcgcgcgac cagttcctcg agcttcggat
     3721 cgacgaagaa atttcgccag cccacgtcgg cgacggcttg taccgccggt accgcggtcg
     3781 acgacgggct cgccggcgtc gccccttcgg tggtcggcgg cagcggccac tgctccggta
     3841 cgccgggccg ggcttcgggc acgcgtggct cgagcacccc gcaggcgctg acgagcgcgg
     3901 cgaggcccgt ggccagcgcc gcccgcaacg gtcgaacgtg cgtcatgccg cacctccggc
     3961 cggggccgcc tggctcgtgg tcggatgggg ttccttgcgg accagccagc gctcgaccac
     4021 gacgaagaac aacgggatga agaccacgcc cagcaacgtg cccacgatca tgccgccgag
     4081 cacgcccgtg ccgatcgcac gctgcgcacc ggaaccggcg cccgacgcga gcgcgagtgg
     4141 caacacgccg aacccgaacg cgagcgaggt catcaggatc gggcgcaggc ggatcttggc
     4201 ggcctgcatc gtgccttcga gcaggtccat gcccttctcg tagttcgact tggcgaactc
     4261 gatgatcagg atcgcgttct tgctcgtgag gccgagcgtg gtgagcatcg cgacctggaa
     4321 gtacacgtca cgctgcagcc cgaacagcgt gctcgcaaga gtcgcgccga ggatgccgag
     4381 cggcgccacc atcagcaccg cggtcgggat cgaccagctt tcgtacagcg ccgccaggca
     4441 caggaacacg atcgcgaacg acagcgcgta cagcagcggc gtctgtgcgc cggcggcacg
     4501 ctcctggtac gaggctccgc tccactcgat gccgaacccg ggcggcagct cgccgaccag
     4561 acgctcgatc tcgtccatgg cctggcccgt gctgacgccc ggagcggcct cgccctggat
     4621 ctcgcgtgcc gaaacgccgt tgtatcgttc caggcgcggc gagccgtatt cccaatgcca
     4681 ggtcgcaaac gacgacaccg ggaccatcaa gccttcggaa ttgcgcaccg accagcgccg
     4741 gaagtcttcc ggcgacatcc ggaacggcgc atcggcctgc aggtagacgc gcttcacacg
     4801 gccgcgatcg atgaagtcgt cgatgtagct gccgccccac gcggccgaca gcgtcgcgtt
     4861 gatgtcggcg atggcgacgc ccagcgcggc ggccttggcc gtgtcgatgt cgagcttgaa
     4921 ctgcggagtg tcctcctggc cgttgggccg cacgttcgcg agcaccttgc tctgcgccgt
     4981 catgccgagc agctggttgc gcgcggccat cagcgcgtcg tggccgttgc cggcattgtc
     5041 ctggagatag aacgtgaagc cggtcgcgac gcccagttcg ggaaccggtg gcggcgcgaa
     5101 cgcgaacgcc atcgcgtcct tgatctgcga cagcgcgccc atcgcccggc gggccacggc
     5161 gctcacgtgc aggtcgtcgg aatcgcgctc gtcccagggc ttcaggttca cgaacgccat
     5221 gcccgagttc tggccaccgc cggcaaagct gaacccgacg atgctgaaga tcgacttgaa
     5281 ggccggttcg ttctccatga agtgatgctc gagtctggcg atcgactgca gcgtccgctg
     5341 ctgcgtcgag ccgaccggcg cctgcacgat ggagatcagc gagccctggt cctcgtcggg
     5401 caggaacgcg ctcggcaggc gcaggaacag caggaccatg acggccgcca acccgaggta
     5461 cacgagcagg aagcgacccg tgcgcgagag cacccgccgc acgatacctt cgtagccgtg
     5521 cgaaccgcga tcgaacgcgc ggttgaacca gccgaagaag ccgcgcgcct cgtggtgctc
     5581 gccgcgacgg atcggcttga gcagcgtcgc gcagagcgcg ggcgtcagca cgatcgccac
     5641 gagcacggac agcgccatcg cggacacgat cgtggcggtg aactggcggt agatcacgcc
     5701 gaccgaaccg gacaggaacg ccatcgggat gaacacggcc gcgagcacca ggccgatcgc
     5761 cacgagtgcg ccggtgatct ggtccatcga cttgcgcgtc gcttcgagcg gcgacagccc
     5821 ctcctcgcgc atcagtcgct cgacgttctc gaccaccacg atcgcatcgt ccacgagcag
     5881 gccgatagcc agcaccatcg cgaacatcgt cagcacgttg atcgagaagc cgagcgccgc
     5941 cagcacgccg aacgtgccga gcagcacgac cgggacggcg atggtcggga tcagcgtggc
     6001 gcggaagttc tgcaggaaca ggtagatcac gaggaagacc agcacgatcg cctcggccag
     6061 cgtcttcacc acctcgatga tggccgcgcg cacgaacggc gtcgtgtcga acgggatcac
     6121 cgtctccatg ccggccggga agaacggccg cagttcgtcg aggcgctcgc gcaccgcgtt
     6181 cgaggtcgca agcgcgttcg cgccggtggc gagcgagatc gccatgccga ccgccggctg
     6241 gccgttgaac cgggtgatcg cctcgtagct ctcggcgccg agttcgacgc gggcgacctc
     6301 gccgagccgc agcgaggagc cgtcgggatt gctgcgcacg acgatgttgc ggaactgctc
     6361 cggcgtctgc aggcgcgact gggcactgat ggtcgcgttg agctgctggc cttccaccgc
     6421 cggcgcgccg ccgagctggc cgaccgtgac ctgggcgttc tgcgcgcgca cggccgtggt
     6481 gatgtcgttg accgacaatc ggtaggtgtc gagcttgttc ggatcgagcc agatccgcat
     6541 cgcgtacggg gcaccgaaca gctgcaccgt gccgacgccc tcgacgcgac tcaacggatc
     6601 acgcaggttg gcaccgacgt aatccgcgag gtcctcgcgg ttcatgctgc cgtccttcga
     6661 gacgaacccg at
//
LOCUS       DASPFR010000106         3373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1374574, whole genome shotgun sequence.
ACCESSION   DASPFR010000106 DASPFR010000000
VERSION     DASPFR010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374574"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..3181)
                     /locus_tag="VD737_02690"
     CDS             complement(74..3181)
                     /locus_tag="VD737_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXW09494.1"
                     /translation="MRIWAFAVRRWQVTLLLFGLLIAVGVSTLQNIPRAEDPEFHAPV
                     PIIVVAYPGADPADIERLIVDPIEDAISELDDIKRMDSRSLDGVGIIQVEFQWDQDPD
                     EKYDEVVREVNRIRPDLPPDIADLEVRRTGSGLVNIMQVALVSDTASYRELDELGRTL
                     RDDVETVPGVRRAEVFALPEPEVRVAVDLERMGRAGVTLGQMESAIRGQNAAIPGGAV
                     DVGLRKFNVKTSGSYDSLDEIADTVVGGRNARIVRLRDVADVTWDTAEQLYVGRFNGR
                     RAIFLAASAKDRVDVFDVRDGIEAKLAAFEQRLPPEVKLERGFDQTRNVGHRLRRLGL
                     DFGIAITLVLLTLVPLGLRAASVVMISIPLSLSIGLAALYFSGFSLNQLSIAGFVLAL
                     GLLVDDSIVVVENIARYLRAGYSRVEAAIAATDQIALAVLGCTATLLFAFLPLLFLPE
                     GSGLFIRSLPAAVLYTVAASLFVALTVIPFLASRVLAVTPAAGEPHGGEGNRALQAIM
                     RFIHGIYAPALRVALAWPRRTLLAALAFVILSFGVAAALGFSLFPNADIPQFRIEVET
                     PTGASVADTDRAVRFIERELARHDEIRHWFANVGHGNPRVYYNIFPEETKANTGEVFI
                     ELDRFDPKRTPALLEELRAKFDAYPGAHIIVEPYRNGPPINAPIEIAVVGPDLDELQR
                     LAREAEALIAATPGARDVDNPARRLRTDLDLNIDTDKAALLGVAPVEVDRTVRAAVAG
                     LTVGKFRETDGDEYDITLRLPMQGRQQLGALDYIQVASASGQPVPLRQLTNPAFSTAP
                     SSIRRHDRLREIIVTAYTTADANVAAVTQDVLGRLERGLDLPPGYSLRAGGELEAQQE
                     TFGGIGTAVLVAVFGILAVLVLEFGSFRSMLIVAGVIPLGMAGGFLGLWVTGYDLSFT
                     ATIGLVALIGIEIKNSILLVDFTNQLREQGLPLDDAVTQAGEIRFLPIILTSATAIGG
                     LLPLAVQGSGLYSPLAIVIIGGLLSSTLIGRLVTPVMYKMLPPRIEVREGAVVDASLA
                     KQASV"
     gene            complement(3184..>3373)
                     /locus_tag="VD737_02695"
     CDS             complement(3184..>3373)
                     /locus_tag="VD737_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09495.1"
                     /translation="AIVYVIDREHNVARRKEVTLGPILGEQVVVTAGLSVGEPVITDG
                     ATWLTDGRAVREVGADQG"
BASE COUNT          544 a         1176 c         1166 g          487 t
ORIGIN      
        1 tagtgttcag tcggaagcgg cttcgccgcc cgccaatccg gccggcctcc tactaaccgc
       61 tagacactga acactagaca ctcgcttgct tcgcaagcga ggcatccacc accgcccctt
      121 cgcgcacctc gatgcgcggc ggcagcatct tgtacatcac cggcgtgacc agccggccga
      181 tcagcgtgga cgacagcagc ccgccgatga tcacgatcgc gagcggcgag tagagccccg
      241 aaccctgcac ggcgagcggc agcaggccgc caatcgcggt ggccgaggtc aggatgatcg
      301 gcaggaaacg gatctcgccg gcctgcgtga cggcgtcgtc gagcggcagg ccctgctcgc
      361 gaagctggtt cgtgaaatcc accagcagga tcgagttctt gatctcgatg ccgatcaacg
      421 ccacgagtcc gatcgtggca gtgaacgaga ggtcgtagcc ggtcacccac agcccgagga
      481 agccgcccgc catgccgagc gggatcacgc ccgcgacgat cagcatcgag cggaaactgc
      541 cgaactcgag cacgagcacg gcgaggatgc caaacaccgc gaccagcacc gccgtgccga
      601 tgccgccgaa cgtttcctgc tgcgcctcga gctcgccgcc cgcgcggagc gagtagcccg
      661 gcggcaggtc gagcccccgc tcgagccggc cgagcacgtc ctgcgtgacc gccgcgacgt
      721 tcgcgtcggc cgtcgtgtag gcggtcacga tgatctcgcg caggcggtcg tggcgccgga
      781 tcgaactcgg cgcggtcgag aacgcggggt tcgtgagctg gcgcagcggc accggttgcc
      841 cgctggcgga agccacctgg atgtaatcga gcgccccgag ctgctgtcgc ccctgcatcg
      901 gcagccgcag cgtgatgtcg tactcgtcgc cgtcggtctc ccggaacttg ccgacggtga
      961 gcccggccac cgcggcacgg acggtccggt cgacctccac cggcgcgact ccgagcagcg
     1021 ccgccttgtc ggtgtcgatg ttcaggtcga ggtcggtgcg cagtcgccgg gccgggttgt
     1081 cgacgtcgcg ggcgccgggc gtcgccgcaa tgagagcctc tgcctccctg gcgaggcgct
     1141 gcagctcgtc gaggtcggga cccaccaccg cgatctcgat cggcgcgttg atcggcgggc
     1201 cgttgcggta gggctcgacg atgatgtgtg cgccggggta ggcgtcgaac ttcgcacgca
     1261 gttcttccag cagcgccggc gtgcgcttcg gatcgaagcg atcgagctcg atgaacacct
     1321 cgccggtgtt cgccttggtc tcctccggaa atatgttgta gtagacacgc ggattgccgt
     1381 gcccgacgtt ggcgaaccag tgccggatct cgtcgtgccg ggcgagctcg cgctctatga
     1441 acctcacggc gcggtccgtg tccgcgacgc tcgcacccgt cggcgtctcg acctcgatcc
     1501 ggaactgcgg gatgtcggcg ttcggaaaca ggctgaagcc gagtgctgcg gccactccga
     1561 aactcaggat cacgaacgcg agggcggcga gcagcgtgcg acgcggccag gcgagcgcga
     1621 cgcgcagcgc gggcgcgtag atgccgtgga tgaagcgcat gatcgcctgc agcgcgcgat
     1681 tgccctcgcc accgtgcggc tcacccgccg ccggcgtcac ggcaagcacg cggctggcca
     1741 ggaacgggat gaccgtcaac gcgacgaaga gcgaggcggc caccgtgtac agcacggcgg
     1801 ccggcagcga gcggatgaac aggcccgagc cttccggcag gaacagcagc ggcaggaacg
     1861 cgaacagcag cgtggccgtg cagccgagca ccgccagcgc gatctggtca gtcgcggcaa
     1921 tcgccgcctc gaccctcgaa tacccggcgc gcaggtagcg ggcgatgttc tcgacgacca
     1981 cgatcgagtc gtcgacgagc aggccgagcg ccagcacgaa cccggcgatc gacagctggt
     2041 tcagcgagaa gccgctgaag tagagcgcgg cgaggccgat cgacagcgac aacgggatcg
     2101 agatcatgac cacgctcgcc gcccgcaggc cgagcggcac cagcgtgagc agcacgagcg
     2161 tgatcgcgat gccgaagtcg aggccgagcc gccggagccg gtggccgacg ttgcgggtct
     2221 ggtcgaagcc gcgctcgagc ttcacttcgg gcggcaggcg ctgctcgaac gccgcgagct
     2281 tcgcctcgat gccgtcgcgc acgtcgaaca cgtcgacccg gtccttggcg ctcgcggcca
     2341 ggaagatcgc acgccggccg ttgaagcggc ccacgtagag ctgctcggcc gtgtcccagg
     2401 tcacgtccgc gacgtcgcgc aagcgcacga tgcgggcgtt gcgcccgccg acgaccgtgt
     2461 cggcgatctc gtccagcgaa tcgtagctgc ccgaggtctt gacgttgaac ttgcgcaggc
     2521 ccacgtcgac ggcgccgccc gggatcgccg cgttctggcc gcggatcgcg ctctccatct
     2581 ggccgagcgt gacgcccgcg cgacccatgc gctcgagatc gacggcgacg cgcacctccg
     2641 gctccggcag cgcgaacact tcggcacggc gcacgcccgg cacggtctcg acgtcgtcgc
     2701 gcagcgtgcg gccgagctcg tcgagttcgc ggtagctcgc cgtgtcgctg acgagcgcga
     2761 cctgcatgat gttgacgagc cccgagccgg tgcggcgcac ctcgaggtcc gcgatgtcgg
     2821 gcggcaggtc cgggcggatc ctgttcacct cgcggacgac ctcgtcgtac ttctcgtccg
     2881 ggtcctggtc ccactggaac tcgacctgga tgatgccgac gccgtcgagc gatcggctgt
     2941 ccatgcgctt gatgtcgtcg agctcgctga tcgcgtcctc gatcgggtcc acgatcaggc
     3001 gctcgatgtc ggccggatcg gcgccgggat acgcgaccac gatgatcggc accggcgcgt
     3061 ggaactcggg gtcctcggcg cgcgggatgt tttgcagcgt gctgaccccg accgcgatca
     3121 gcaggccgaa cagcagcagc gtgacctgcc agcgacggac cgcgaacgcc cagatccgca
     3181 cgctcagccc tggtcggcgc cgacttcgcg caccgcgcga ccgtcggtga gccacgtcgc
     3241 gccgtcggtg atcaccggct caccgacgct gaggccggcg gtcacgacga cctgctcgcc
     3301 gaggatcggg cccagcgtca cttccttgcg gcgcgcgacg ttgtgctcgc ggtcgatgac
     3361 gtaaacgatc gca
//
LOCUS       DASPFR010000107         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1498049, whole genome shotgun sequence.
ACCESSION   DASPFR010000107 DASPFR010000000
VERSION     DASPFR010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498049"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VD737_02700"
     CDS             complement(<1..581)
                     /locus_tag="VD737_02700"
                     /inference="COORDINATES: protein motif:HMM:NF024846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HXW09496.1"
                     /translation="MNYIIVGAGFAGCVLAERLARGSGERVWIVEKRPHIGGNAYDHY
                     DDAGILVHKYGPHIFHTNSREVFQYLSRFTEWRPYEHRVRASVDGQLVPIPINLDTVN
                     ALYGLSLTSSEVESFFASVAEARTPALTSEDVIVSKVGRELYEKFFRGYTRKQWGLDP
                     SELDAQVTARVPVRTNRDDRYFNDVYQAMPRYG"
     gene            complement(663..2432)
                     /locus_tag="VD737_02705"
     CDS             complement(663..2432)
                     /locus_tag="VD737_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HXW09497.1"
                     /translation="MSGDAVSPRGYPRPLLRRAQWVSLDGAWDFAGDPDRQWLLPGEV
                     IWGERIRVPFSPETAASGVDRTEYLPRCWYRTTITPPTSQGPLHIHFGAVDYHATVWL
                     DGELRGRHEGGYTPFTIDATPAARASAGQPIEIVVRVDDDPHDLSKPRGKQDWQPDPH
                     SIWYHRTTGIWQSVWLEPLPPTWIAALRWRASLLNWEIRLHAGIAGELTRPLSLRVRL
                     SHAGRLLSGDTYSVVTEELSRGITLTDPGIDDFRNELLWSPGSPSIIDAELELLDEAG
                     RVVDHVFSYTALREVGVSGDRFMLNGRAYPLRLVLDQGYWPASGMTAPSDEALRRDVE
                     LALAMGFNGVRKHQKVEDPRYLYWADHLGLLVWAEMPSAYRFTADSVRRLTVQWMEVL
                     ARDGSHPCIVSWVPFNESWGVPNLPNEASHRHYVRALYHLTRTLDPSRPVVGNDGWES
                     VATDIIGIHDYDANPQALRERYRSDEVLPRLFRRERPGGRVLVLEGHSQPDQPIVLSE
                     FGGIACSRGDNWSWGYSRCHTEAELQERFAQLMDVVTRLNVFAGFCYTQFADTYQEAN
                     GLLYDDRTPKMPLAEIAAAVRGR"
     gene            complement(2466..>3088)
                     /locus_tag="VD737_02710"
     CDS             complement(2466..>3088)
                     /locus_tag="VD737_02710"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXW09498.1"
                     /translation="VAGGVALAAAARASRGRIPLAAASAALLVRAATGYCPIVARTRN
                     GDSETRRRLGGPRGAHLQAQARIERPPDEVYAFWRDLDRLSAALPRLVSAVELDTTRS
                     EWSLGDRARWTAEIINDEPGRVIGWQTLSGADIVSAGSVRFNEAGNDATDLVVHMQYS
                     PPFGRLGASLAELFGKGATDIVEESLAAAKRAIESSTPHLATEPSR"
BASE COUNT          491 a         1042 c         1068 g          487 t
ORIGIN      
        1 tagccgtatc gtggcatcgc ctggtagacg tcgttgaagt agcggtcgtc cctgttcgtg
       61 cgcaccggca cgcgcgcagt cacctgcgcg tcgagttcgg acgggtccag cccccactgc
      121 tttcgcgtgt agccgcggaa gaacttctcg tagagctcgc gtccgacctt cgagacgatc
      181 acgtcctcgg aggtcaacgc cggcgtgcgc gcctcggcga cgctcgcgaa gaacgactcc
      241 acttccgacg aggtcagcga aaggccgtac agcgcattca cggtgtcgag gttgatgggg
      301 atcggcacca gctgcccgtc gacgctcgcg cggacgcggt gctcgtaggg ccgccattcc
      361 gtgaacctcg agaggtactg aaacacctcc ctggagtttg tatgaaagat gtgggggccg
      421 tatttatgaa cgaggatgcc ggcgtcgtcg tagtggtcgt acgcgtttcc cccgatgtgc
      481 ggacgcttct ccacgatcca gacgcgctcg cccgagcctc gggccagccg ttctgcaagc
      541 acacagccgg caaagccggc accgacgatg atgtagttca cagccacgtc cagtcgaaag
      601 cgggagcctc atccgtggag tgcaagtgag gtgccgatcg cggcgcgctc gagcgtccca
      661 catcaccgtc cgcggaccgc ggccgcgatt tcagccaacg gcatcttcgg cgtgcgatcg
      721 tcgtagagca gcccgttggc ttcctggtag gtgtcggcaa actgggtgta gcagaagcct
      781 gcaaacacgt tcagccgggt cacgacatcc atcagctgag caaagcgctc ctgcagttcg
      841 gcctcggtgt ggcatcgcga gtagccccag ctccagttgt cgccgcgcga gcaggcgatg
      901 ccgccaaact ccgagagcac gatcggctgg tccggctggc tgtgcccctc gagcacgagc
      961 acgcgcccgc ccggacgctc gcgcctgaac aggcgcggca gcacctcatc cgagcgatag
     1021 cgttcgcgca gggcctgcgg gttggcgtcg tagtcgtgaa tgccgatgat gtcggtggcc
     1081 acgctctccc agccgtcgtt gccgacgaca ggacgggacg ggtcgagcgt gcgcgtgaga
     1141 tggtagagcg cgcggacgta atggcgatgc gacgcctcgt tcgggaggtt gggcacgccc
     1201 cacgactcgt tgaacggcac ccacgacacg atgcacgggt ggctgccgtc gcgcgccagc
     1261 acttccatcc actgcaccgt caggcggcgc accgagtcgg ccgtgaagcg gtacgcgctc
     1321 ggcatctcgg cccagacgag caggccgaga tggtcggccc agtagagata gcgcgggtcc
     1381 tcgaccttct ggtgcttgcg cacgccgttg aatcccatgg ccagcgccag ctcgacgtcg
     1441 cgtcgcagcg cctcgtcgct cggggccgtc atacctgacg ccggccagta gccctggtcg
     1501 aggacgagcc gaagcggata ggcacgtccg ttcagcatga agcgatcgcc gctcacgccg
     1561 acttcgcgca gcgccgtgta gctgaagacg tggtcgacga cacggccggc ctcgtcgagc
     1621 agttcgagct cggcgtcgat gatcgagggc gacccgggac tccacaacaa ctcgttgcgg
     1681 aagtcgtcga tgcccgggtc ggtgagcgtg atgccgcgcg acagttcttc ggtgacgacc
     1741 gagtaggtgt cgccgctcag caggcgtccc gcgtgcgaga gacgcacgcg cagcgacaac
     1801 ggccgcgtca gctcgccggc aatgcccgcg tgcaggcgga tctcccagtt gagcaggctc
     1861 gcgcgccatc gcagcgctgc tatccaggtc ggaggcagcg gctcgagcca gaccgactgc
     1921 cagatgccgg tcgtccggtg ataccagatc gagtgggggt cgggctgcca gtcctgcttg
     1981 ccgcgcggct tggacagatc gtgcgggtcg tcgtcgaccc ggacgacgat ctctatgggc
     2041 tgtccggcgg acgcacgcgc cgccggcgtg gcatcgatgg tgaacggcgt gtagccgccc
     2101 tcgtgccgtc cgcgcagctc gccgtcaagc cagacggtgg cgtggtagtc gacggcgccg
     2161 aagtggatgt gcagcgggcc ctgcgacgtc ggcggtgtga tggtcgtgcg ataccagcag
     2221 cgtggcagat actccgttct gtccacgccg cttgccgccg tctccggaga gaacggcacg
     2281 cggatgcgct cgccccagat aacctcgccc ggcagcagcc actgacgatc gggatcgccc
     2341 gcgaagtccc acgcgccgtc gagcgacacc cactgggcac gtcgcagcag gggccgcggg
     2401 tacccccgtg gcgagacagc gtcaccgctc attggtcttt cgcgacgctc gatgtgccgg
     2461 gacggctacc tcgacggctc tgtcgccagg tgcggcgtcg agctctcgat ggcgcgcttg
     2521 gctgccgcga ggctctcttc gacgatgtcg gtggcgccct tgccgaacag ctcggcgagg
     2581 ctggcgccaa gacggccaaa cggcggcgag tactgcatgt gcacgaccag gtcggtggcg
     2641 tcgttgccgg cctcattgaa gcggacgctg ccggcactga cgatgtcggc gcccgagagc
     2701 gtctgccagc cgatgacccg gccgggttcg tcgttgatga tctccgccgt ccaccgcgcg
     2761 cggtcgccga gcgaccattc cgaacgcgtc gtatcgagtt cgaccgcgct gaccagacgc
     2821 ggaagcgcgg ccgacagccg gtcgaggtcg cgccagaagg cgtacacctc gtccgggggc
     2881 cgctcgatcc gcgcctgcgc ctgcaagtgc gcgccgcgcg ggccgcccag gcgtcgcctg
     2941 gtctcggaat cgccgttccg ggtgcgagcg acgatcgggc aataacccgt cgcggcgcgg
     3001 accagcagcg ccgccgaggc ggcggccagc gggattcggc cgcgcgaggc acgcgcggcc
     3061 gccgccaggg ccacgccgcc ggccactg
//
LOCUS       DASPFR010000108         2131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_887334, whole genome shotgun sequence.
ACCESSION   DASPFR010000108 DASPFR010000000
VERSION     DASPFR010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887334"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1777)
                     /gene="argS"
                     /locus_tag="VD737_02715"
     CDS             complement(32..1777)
                     /gene="argS"
                     /locus_tag="VD737_02715"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HXW09499.1"
                     /translation="MIEQLVQDALRALPDDLRPGDAASVVAVERTRDASHGDFASNVA
                     LQLAKAARRKPRDLAQAIVAAMPASELVAKVDIAGPGFINFFLTPMACQTELLRVLDG
                     GRSYGRSQAGGGKRVLVEFVSANPTGPLHVGHGRHAAFGASLANLLDAAGYAVSREYY
                     INDAGRQMDILATSVWLRYLESFGEQFPFPSNGYRGDYIGPIANKLIERHGRALWRPA
                     AEVFANLPQDAPAGDQDEYIDAVIDRARQLLGPERFRQAFDLALGDILDDIRDDLAEF
                     GVRYDLWFSERSLAEQGAIDHALDLLRSNGVVYGKDGAEWFRATDFGDEKDRVVVREN
                     GAKTYFASDIAYHLNKCERGFEQLIDVLGSDHHGYIARVRAGLLAMGQSGDCLDVRLM
                     QFVTLYRGGEKVQMSTRSGEFITLRELRREVGNDAARFFYVMRSNEQHLDFDMQLAVS
                     RSNDNPVYYIQYAHARVCSVLRQVREKGLGHDEARGRASLGRLVEPHEQAVLATLSRF
                     PEVVEAAAEQRAPHMLVTYLRELATEFHAYYNAHQFLVPDEALRDARLLLIRGVRQVV
                     CNGLGLLGVSAPEAM"
BASE COUNT          346 a          729 c          719 g          337 t
ORIGIN      
        1 ctcagcgtgc gggcgcccgt gctcgttgcc atcacatcgc ctccggcgcc gaaacgccga
       61 gcagcccgag cccgttgcag acgacctggc gtacgccgcg gatcagcagg agccgtgcat
      121 cgcgcaacgc ctcgtcgggc acgaggaact ggtgcgcgtt gtagtaggcg tggaactcgg
      181 tggcgagttc ccgcagatac gtgacgagca tgtgcggcgc ccgctgctcg gccgcggcct
      241 cgaccacttc cgggaagcgg ctcagggtgg cgagcaccgc ctgttcgtgg ggctcgacca
      301 gccggccgag gctcgcccga ccgcgtgctt cgtcgtgccc gaggcctttc tcgcgcacct
      361 gccgcagcac gctgcacacg cgcgcgtgcg cgtactggat gtagtagaca gggttgtcgt
      421 tggaccgcga gaccgcgagc tgcatgtcga agtcgaggtg ctgctcgttg ctgcgcatga
      481 cgtagaagaa ccgcgccgca tcgttgccga cctcgcgccg cagttcgcgc agcgtgatga
      541 actcgcctga gcgcgtcgac atctgcactt tctcgccgcc gcggtacagc gtcacgaact
      601 gcatcaggcg cacgtccagg cagtcgccgg actgccccat cgcgagcagg ccggcccgca
      661 cccgggcgat gtagccgtgg tgatcggagc cgagcacgtc gatcagctgc tcgaagccgc
      721 gctcgcactt gttcaggtga tacgcgatgt cggacgcgaa atacgtcttc gcgccgtttt
      781 cacgcacgac cacgcggtcc ttctcgtcgc cgaagtccgt ggcgcggaac cattcggcgc
      841 cgtccttccc gtagacgacg ccgttgctgc gcagcaggtc gagcgcgtgg tcgatcgcgc
      901 cctgctcggc gaggctgcgt tcgctgaacc acaggtcgta ccggacgccg aactcggcca
      961 ggtcgtcgcg gatgtcgtcg aggatgtccc cgagggcgag gtcgaaggcc tgccggaacc
     1021 gctcagggcc gagcaactgc cgcgcgcggt cgatcacggc gtcgatgtac tcgtcctggt
     1081 cgccggccgg tgcatcctgc ggcaggttcg cgaacacctc ggcggccggg cgccacagcg
     1141 cgcgcccgtg gcgctcgatc agcttgttgg cgatgggccc gatgtagtcg ccgcgatagc
     1201 cgttgctcgg gaacgggaac tgctcgccga agctctcgag gtaacgcaac cacacgctgg
     1261 tcgcgaggat gtccatctgg cggcccgcgt cgttgatgta gtactcgcgc gagaccgcgt
     1321 acccggctgc atcgagcagg ttcgcgagcg acgcgccgaa cgccgcgtgc cgcccatgac
     1381 cgacatgcag ggggccggtc ggattggcgg acacgaactc gaccagcacg cgcttgccgc
     1441 ccccggcctg cgatcgacca taggaacgcc caccgtcgag caccctgagc agctcggtct
     1501 ggcaggccat cggcgtcagg aagaagttga tgaagcccgg cccggcgatg tccactttcg
     1561 cgacgagctc gctcgcgggc atcgccgcca cgatcgcctg ggccaggtcg cgaggcttgc
     1621 gcctggccgc tttcgcgagt tgcagcgcca cgttcgaggc gaagtcgccg tgactggcat
     1681 cgcgggtgcg ctcgacggcg acgacggacg cggcgtcacc aggacgcagg tcgtcgggca
     1741 gggcgcgcaa ggcgtcctgc accagctgtt cgatcacgta tctcaaggaa ctcgccgtca
     1801 ggtcgcgcag ggcacgcgaa gtctagggaa ttccagacga cgatgcgagc aaagcgtgac
     1861 cgcgaccggc gacgcatcgc gatccttgct gcgctcagcc gtgcactaag actcgtccag
     1921 cttcgacacc agcacccacg atcggccggc ctccgcggac ggcctgtagg tcgacacgga
     1981 cgcgagcgaa ggcccgtcgg cgcgcgtctg ttcgaaaggg acgccggaaa aaatttcaat
     2041 tacatccggt agttggcgcg cctcctcgtg ttttcgcgat gtcgccaaag gcagacacct
     2101 cgaccccgga acacggtccg cgaaccccgg t
//
LOCUS       DASPFR010000109         8847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_521450, whole genome shotgun sequence.
ACCESSION   DASPFR010000109 DASPFR010000000
VERSION     DASPFR010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8847
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521450"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="VD737_02720"
     CDS             complement(<1..1345)
                     /locus_tag="VD737_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09500.1"
                     /translation="MVNAGRTVAGWLAGRALLFCLALIVLVAVDVSRDEGSWIRARVV
                     SVLPDARSAADLSRRRAATLQQAEAWRRQFNSRLADAHTLPAGELARWLGALDREIAD
                     LESQRLGVLDLVLAADDPGRLVEHTRNEARLQVLRWSRDELRRLAALRTVTLSPREAD
                     ERARSARARHVEATRRYWAARRVHDQYAREHPIAAAVPFEAFGLHSADRDAYLALRRV
                     YVDAAAELRAAKRRRDGAEALQQRLAQAQPLVARPLARIDESATQALDAVIEARRRSA
                     AAMAHGLVELWRRIGQVATAAFWIVVVLTLLPVALKAFWYYVMAPMAARRPPIRIAPG
                     TTPTTVDAAGPSACISAVSLDVTLGAHDELLVHPEYLQSLATPGHKSTRWLLDPRYPL
                     MSLASGMVALTRIRGARQVYTVSSRKDPLAEVAAVDVPAGAQFVLQPRRLVGVVQD"
     gene            complement(1414..2862)
                     /locus_tag="VD737_02725"
     CDS             complement(1414..2862)
                     /locus_tag="VD737_02725"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXW09501.1"
                     /translation="MLKQLTARKSIAMIHRELESGPKLNRVLDRRALTAVGLGSMIGS
                     GIFVLTGTVASLHTGPAVTLAFLVAALGCGLAAMCYAELASMIPVSGSAYSYTYATLG
                     EGLAWFVGWNLALEYVMSGAAVAVSWSGYVVNLLAEFGVHLPSALTNAPLAKVDGSLH
                     HLGFTGAIVNLPAVLIVVALTYLCYVGVRQSARANNVMVSIKVIIIVLFILVGLQYVS
                     TDLWKPYLPVNTGQFGQFGLSGVLQGAAIIFFAYIGFDQAATTAQEARNPQRDVPWGI
                     MAALGISTLLYISMAAVMTGMVPYAQLNVSAPVAVALDAHPELRWLALPVKVGIIVGL
                     TSVILMSLLGQPRIFLAMSRDGLLPPALQKVHPKHRTPYVGTVVTGVVACFFAGLFPL
                     DILGELISIGILIAFSAVCAGVVVLRYTQPETPRPFRVPLAKVTGPAGVVVCFTMAYA
                     LPHDTWIRLIGWTAIGFAIYFGYGYHHSRLRA"
     gene            2957..4444
                     /locus_tag="VD737_02730"
     CDS             2957..4444
                     /locus_tag="VD737_02730"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HXW09502.1"
                     /translation="MMTRSMPNDRGDAPPRPVEDCYWLLPGRLLVGAYPGSRSRAQAM
                     DRLRRLLEAGVTCFVDLTEPDEIASYETLLPFETPGGRRVEHLREPIVDHGVPASRET
                     MARILAMIDGALDSGHTVYLHCRAGIGRSAMAAGCWLAERSGDGEQALRELAACWPQA
                     TQSQHWPRVPETDEQVRFVLEWPTGRAAARVHQHAGNTDSAAERLRGAWYGLALGDAA
                     CAANVVVDAPVAWTQHTALALCLADSLIDKGGSDARDQIERYLRWQRVGDRTATGTVA
                     EAIATPDVARALATYLWRRQPMAGAHDPRDASPTSLPRVLAPACFAVADPAAAVAVAA
                     EAARTTHQSPLILEACRLYAAMLVGALRGEPPSRWLESVPAFEPSPWTAKPLRKDVLA
                     IASAERVDATLAPQGTVLHVLLEARRIAQQARDFGSVIEAARLAARKDQATAPYGALA
                     GTLYGAMHGSTAITAEARARLAGNDQLDAAIERWLVRGRTAGATA"
     gene            4441..5475
                     /locus_tag="VD737_02735"
     CDS             4441..5475
                     /locus_tag="VD737_02735"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HXW09503.1"
                     /translation="MKPRKPRREPAWTRLDDEALLDMRLCDLRLSLRSSGLDHAVHRL
                     YEELRLKGIRFRPHVWISEEWFSPDGTPGIAIPFYLGHPRLQRLERRFMHEVEGGNDK
                     WLMRILRHEAGHAIDTAFTLRRRKTWRQAFGKASRRYPTRYFPRPASRRFVLHLGHWY
                     AQSHPTEDFAETFAVWLAPRSRWRSQYAGWPAFEKLEYVDRTMRDLEGRRATCLNRDV
                     VEPLAENTRTLREHYRRKQRRYEMDTARTYDRRLLRVFGTAESHPRGANAGRFLRKVQ
                     PQLKRLLVRRARMHPYLVQHVLRTATQRARHMNLRLRHSQRNTKRELVVMLERIMLDM
                     LLRDRETYAL"
     gene            5472..6503
                     /locus_tag="VD737_02740"
     CDS             5472..6503
                     /locus_tag="VD737_02740"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09504.1"
                     /translation="MNRKPMRVLVLVHETLVPPETTDGYTQQEIDEWQTEFDVTTSLR
                     AMGHDVRVIGLGDNLAELRATILDWKPDIAFNLLEEFQGIVTYDTYVVAFLELMRLPY
                     TGCNPRGMMISRDKVLSKQILAYHRIPTARYALLPRKRRYAEPRRLKFPLFVKSATED
                     ASLGIAQASIVYDSPKLKERVEFIHEQTNSDALVEEYIEGRELYIGVMGNERLTAFPV
                     WELNFGTLPEVMSIATRKVKWDRRYQQKHGIDTGPARDLPPGMSTRLAGLARRIYRAL
                     SLSGYARIDVRLKPDGSVYVLEANANPNLAQDEDFATSALSAGMGYDTVLQRIIQCGE
                     NYDAAWRES"
     gene            complement(6606..7022)
                     /locus_tag="VD737_02745"
     CDS             complement(6606..7022)
                     /locus_tag="VD737_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HXW09505.1"
                     /translation="MTTATTTLDDLDRRQVEALRAAMLERARQLREEIRQTLLKSDSE
                     QYLQIAHDVRDLEDESFADLIVDVSLAEIDRDLEELRVIDAALLRVADGSYGTCSVCD
                     RPIEVRRLEATPHAARCIDCQTVYERTHFSRTGSTL"
     gene            complement(7208..7744)
                     /gene="def"
                     /locus_tag="VD737_02750"
     CDS             complement(7208..7744)
                     /gene="def"
                     /locus_tag="VD737_02750"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXW09506.1"
                     /translation="MAIRPVLRMGHPLLQRVAEPIERFGTEELRELVRDMDDTMRSLD
                     GAGIAAPQIGVSWRVVIFEVTANPRYPQAETVPYTVLVNPALEPLGTEQEDAWEGCLS
                     VPGLRGLVPRYRRLRYRGYDPEGRPIDRTVSGFHARVVQHEVDHLDGILYPMRVADLR
                     NLGFEDVLFPGARNLPPD"
     gene            7833..8135
                     /locus_tag="VD737_02755"
     CDS             7833..8135
                     /locus_tag="VD737_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09507.1"
                     /translation="MDHGLRCWKCGGSLADYSLPLRRIEECRACRAELHVCRMCEFYD
                     TSKAKHCRETIADEVKDKQRANFCDYFKPTPAAWQPGATTAADKARAELEALFGRK"
     gene            complement(8184..>8847)
                     /locus_tag="VD737_02760"
     CDS             complement(8184..>8847)
                     /locus_tag="VD737_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09508.1"
                     /translation="PDRLRLLAEAGESHQADLIADGILFRGARPPGAPAELMTWQRDG
                     PDLERLSAEAMIRSGTGGGRCSLGYLKPLMRRRFLDERKLRYAEDLRFAEDFNLYVHA
                     LFQGARFLLYPESHYVYWQTPSSASRTEISRIARHALEGSQRLRASVPQGHSSALVTA
                     LDDYEQRWLLLCWFEQLKRGVTHRRLGHLVELLFKPPASPSRILRFARDRARHRREAL
                     RC"
BASE COUNT         1405 a         3039 c         3155 g         1248 t
ORIGIN      
        1 ggtcctggac cacgccgacg agccggcgcg gctgcagcac gaactgcgca cccgccggca
       61 cgtccaccgc ggccacctcc gcgagcgggt ccttgcggct cgaaaccgtg tacacctgtc
      121 gcgcgccgcg gatccgcgtg agcgccacca tgcccgacgc cagactcatc agcggatagc
      181 gcgggtcgag cagccagcgc gtggacttgt ggccgggcgt tgcgaggctc tgcaggtact
      241 cgggatgcac cagcagctcg tcgtgcgcgc cgagcgtcac gtcgagcgaa acggcggaga
      301 tgcaggccga cggcccggcc gcgtccaccg tcgtcggcgt ggtgcccggg gcgatccgga
      361 tcggcgggcg acgggctgcc atcggcgcca tcacgtaata ccagaacgcc ttcagtgcga
      421 caggcaacag ggtcaacacc acgacgatcc agaatgccgc cgtcgccacc tggccgatcc
      481 ggcgccacag ctcgacgagt ccgtgcgcca tggcggcggc ggatcgtcgc cgggcctcga
      541 tcacggcgtc gagcgcctgc gtggcggatt cgtcgatgcg cgcgagcggg cgtgcgacga
      601 gcggctgcgc ctgcgcgaga cgctgctgca gtgcttcggc gccgtcgcga cgacgcttcg
      661 cggcgcgcag ttccgccgcg gcgtccacgt agacgcgtct cagcgcgagg tacgcgtcgc
      721 gatccgccga gtgcaggccg aacgcctcga atggcaccgc ggcggcgatc gggtgttcgc
      781 gcgcgtattg gtcgtgcacc cgccgcgccg cccagtatcg gcgcgtcgcc tccacgtggc
      841 gtgcccgtgc cgagcgcgcc cgttcgtcgg cttcgcgcgg cgacaacgtc acggtgcgca
      901 gggccgcgag gcgccgcagt tcgtcgcggc tccagcgcag gacctgcagc cgtgcctcgt
      961 tgcgcgtgtg ctcgaccagg cggccggggt cgtccgccgc aagcacgagg tcgagcacgc
     1021 cgagtcgctg cgattcgagg tcggcgatct cgcggtcgag ggcgccgagc cagcgggcga
     1081 gctcgcctgc cggcaaggtg tgcgcgtccg cgagccggga gttgaactgc cgtcgccacg
     1141 cttcggcctg ctgcagcgtc gcggcgcgtc gtcgcgagag gtccgcggcg ctgcgcgcgt
     1201 cgggcagcac cgacacgacc cgcgcgcgta tccacgagcc ctcgtcgcgc gagacgtcga
     1261 cggcgacgag cacgatgagc gccaggcaga acagcagtgc cctgccggcc agccagccgg
     1321 cgacggtgcg gcctgcattg accatcaggg cggcctccgt gccgcgatgc gatagccggc
     1381 gatcagccta cgtctcgggg cctgcgccga tcgtcacgcg cgcaggcggc tgtggtggta
     1441 accgtagccg aaatagatcg cgaagccgat cgcggtccag ccgatcagcc ggatccacgt
     1501 gtcgtgcggc agcgcgtacg ccatcgtgaa acatacgacg acacccgcgg ggcccgtgac
     1561 cttggccagt ggcacgcgga acggtcgagg cgtctccggc tgcgtgtagc gcagcacgac
     1621 gacgccggcg cagaccgcgg agaacgcgat caggatgccg atcgagatca actcgccgag
     1681 gatgtcgagc gggaacagcc cggcgaaaaa gcacgcgacg actcccgtga cgaccgtgcc
     1741 gacgtagggc gtgcggtgct tggggtgcac cttctgcagc gccggtggca acagtccgtc
     1801 gcgcgacatc gcgagaaaga tgcgcggctg gccgagcagc gacatcagga tcaccgacgt
     1861 caggccgacg atgatgccga ccttgaccgg cagcgcgagc cagcggagtt ccgggtgcgc
     1921 gtcgagcgcg acggcgaccg gcgcggagac gttgagctgg gcgtacggca ccatgccggt
     1981 catcacggcg gccatggaga tgtagagcag ggtggaaatg ccgagcgcag ccatgatgcc
     2041 ccagggcacg tcgcgctgcg gattgcgcgc ttcctgcgcc gtggtcgcgg cctggtcgaa
     2101 gccgatgtag gcgaagaaga tgatcgccgc gccctgcagc acgccgctca gcccgaactg
     2161 gccgaactgg ccggtgttga cgggcaggta aggtttccac aggtcggtcg agacgtactg
     2221 gaggccgacc aggatgaaca gcacgatgat gatgaccttg atcgacacca tcacgttgtt
     2281 ggcgcgcgcc gactgccgga cgccgacgta gcacaggtac gtgagcgcga cgacgatcag
     2341 caccgcaggc aggttgacga tcgcaccggt gaagccgagg tggtgcagcg acccgtccac
     2401 cttggcgagc ggggcattgg tcagcgcgga gggcaggtgg acgccgaatt cggccagcag
     2461 gttcacgacg tagcccgacc agctgacggc cacggccgcg cccgacatca catattccag
     2521 cgcgaggttc cagcccacga accaggcgag gccttcgccg agcgtggcgt aggtgtagct
     2581 gtaagcgctg ccggagaccg ggatcatcga ggcgagctcg gcgtaacaca tggcggcgag
     2641 cccgcagccc agggcggcga ccaggaacgc gagcgtcacg gccggcccgg tgtgcagcga
     2701 ggcgacggtg ccggtcagca cgaaaatgcc gctgccgatc atggagccca gtccgacggc
     2761 cgtcagcgcc cggcgatcga ggactcgatt cagcttgggg cccgattcca gctcccggtg
     2821 gatcatcgcg atcgacttgc gggccgtcag ctgcttgagc actgcgtgcg tctccccctg
     2881 ggcgttcggt caagtctctc acagcggccc gccgggccgc accccggccg tggccgtgtc
     2941 ttatgatgcc ggctgcatga tgacgcgcag catgccgaac gaccggggcg acgccccacc
     3001 gcgacccgtc gaagactgtt actggctgct gccggggcgc ctgctcgtcg gcgcctatcc
     3061 gggatcgcgc tcgcgcgcgc aggcgatgga tcgcctgcgg cggctgctcg aggccggcgt
     3121 gacgtgcttc gtcgacctga ccgaacccga cgagatcgcg tcgtacgaaa cgctgctgcc
     3181 gttcgaaacg ccgggcggcc gccgcgtcga gcacctgcgc gagccgatcg tcgaccacgg
     3241 cgtgccggcg agtcgcgaaa ccatggcccg catcctcgcg atgatcgacg gcgcgctcga
     3301 ctccggccac acggtctacc tgcactgtcg cgccggcatc ggccgtagcg cgatggcggc
     3361 ggggtgctgg ctggcagagc gctcgggcga cggcgagcag gcgctgcgcg agctcgcggc
     3421 ctgctggccg caggccaccc agtcgcagca ctggccgcgc gtgcccgaga ccgacgagca
     3481 ggtccgcttc gtgctcgagt ggcctacggg ccgcgccgcg gcgcgcgtgc accagcatgc
     3541 gggcaacacc gactcggcgg ccgagcggtt gcgcggagcg tggtacggac tcgcgctcgg
     3601 cgacgcggcg tgcgcggcga acgtcgtcgt cgacgcgccc gtcgcctgga cgcagcacac
     3661 ggcgctggcc ctgtgcctcg ccgacagcct gatcgacaag ggcggcagcg acgcgcgcga
     3721 ccagatcgag cgctacctgc gctggcaacg cgtgggcgat cgcaccgcga cgggcaccgt
     3781 cgccgaagcc atcgcgacgc cggacgtcgc cagggcgctc gcgacgtacc tgtggcgcag
     3841 gcagccgatg gccggcgcgc acgatcctcg cgacgcctct cccacgagcc tgccgcgcgt
     3901 acttgcgccg gcgtgtttcg ccgtcgccga tccggcggcc gccgttgcgg tggcggcgga
     3961 ggccgctcgc acgacgcacc agtcgccgct gatcctcgag gcgtgccgcc tgtatgccgc
     4021 gatgctggtc ggcgcgctgc gcggcgaacc gccgtcgcgc tggctggaat cggtgccggc
     4081 gttcgagccc tcgccgtgga ccgcgaagcc gttgcgcaag gacgtgctgg cgattgcgtc
     4141 ggccgagcgt gtcgatgcga cgctcgcgcc gcagggcacc gtcctgcacg tgctgctcga
     4201 ggcgcgacgc atcgcgcagc aggcacgcga tttcggcagc gtgatcgagg ccgccaggct
     4261 cgcggccagg aaggaccagg cgaccgcgcc ctacggcgcg ctcgcaggca cgctgtacgg
     4321 cgcgatgcac ggatccacgg ccattacggc ggaggctcgc gcgcgtctcg cggggaacga
     4381 ccagctcgac gcggcgatcg agcgttggct ggtacgcggc cggacggccg gagcgactgc
     4441 ttgaagccgc gcaagcctcg ccgggagccc gcctggacga ggctggacga cgaggcgctc
     4501 ctcgacatgc ggctgtgcga cctgcgcctg tcgctgcgca gctccggcct cgaccacgcc
     4561 gtgcatcggc tctacgagga actgcgcctg aagggcatcc gcttcaggcc gcacgtgtgg
     4621 atctccgagg agtggttctc gcccgacggt acgccgggca tcgccatacc gttctacctc
     4681 ggccacccgc gcctgcagcg gctcgagcgg cgcttcatgc acgaggtcga gggcggcaac
     4741 gacaagtggc tgatgcgcat cctgcggcac gaggcgggcc acgcgatcga caccgcgttc
     4801 acgctgcgcc ggcgcaagac ctggcgacag gccttcggca aggcctcgcg gcgctatccg
     4861 acgcgctatt ttccacggcc ggcgagccgc cgcttcgtgc tgcacctggg tcactggtac
     4921 gcgcagagcc acccgaccga ggacttcgcc gagacgttcg cggtgtggct cgcgccgcgt
     4981 tcacgctggc gcagccagta tgcgggctgg cccgcgttcg agaaactcga gtacgtggac
     5041 cgcacgatgc gcgacctcga gggccgtcgc gccacgtgcc tgaaccgcga cgtcgtcgag
     5101 ccgctcgcgg agaacacgcg cacgctgcgc gagcactacc ggcgcaagca gcgccggtac
     5161 gagatggaca ccgctcgcac ctacgaccgg cgactgctgc gcgtgttcgg cacggcggag
     5221 agccatccgc gcggcgccaa tgccgggcgc ttcctgcgca aggtccagcc ccagctcaag
     5281 cgcctgctcg tgcgtcgtgc gagaatgcac ccttacctcg tgcaacacgt cctgcggacc
     5341 gcgacccagc gtgcgcggca catgaacctg cgcctccgtc acagccagcg caacaccaag
     5401 cgcgagctcg tcgtgatgct cgagcggatc atgctcgaca tgctgctgcg ggaccgcgag
     5461 acctacgccc tatgaaccgc aagccgatgc gcgtgctggt gctcgtgcac gagacgctcg
     5521 tgccgcccga gaccacggac ggctacacgc agcaggagat cgacgagtgg cagaccgagt
     5581 tcgacgtcac gacgtcgctg cgcgcgatgg gccacgacgt ccgcgtgatc gggctcggcg
     5641 acaacctggc cgagctgcgc gcgacgatcc tcgactggaa gccggacatc gcgttcaacc
     5701 tgctcgagga gttccagggc atcgtcacct acgacaccta cgtcgtcgcg ttcctcgagc
     5761 tgatgcgcct gccgtacacc ggctgcaacc cgcgcggcat gatgatctcg cgcgacaagg
     5821 tgctgtcgaa gcagatcctc gcctaccacc gcataccgac ggcgcgctat gcgctgctgc
     5881 cgcgcaagcg ccggtatgcg gagccgcgca ggctcaagtt cccgttgttc gtgaagtcgg
     5941 cgaccgagga cgcctcgctc ggcatcgcgc aagcctccat cgtctacgac agcccgaagc
     6001 tcaaggagcg ggtggagttc atccacgagc agaccaactc cgacgcgctg gtggaggagt
     6061 acatcgaggg gcgcgaactc tacatcggcg tgatgggcaa cgagaggctg acggcgtttc
     6121 cggtgtggga gctcaacttc ggcacgctgc cggaggtcat gtcgatcgcc acgcgcaagg
     6181 tcaagtggga ccgccggtac cagcagaaac acggcatcga caccggtccg gcgcgcgacc
     6241 tgccgcccgg gatgtcgacg cgactcgcgg ggctcgcccg gcgcatctat cgcgcgcttt
     6301 ccttgagcgg ctatgcccgc atcgacgtgc gcctgaagcc cgacggcagc gtctacgtgc
     6361 tggaggccaa cgccaacccc aacctcgcgc aggacgagga cttcgcgacc tccgcgctct
     6421 ccgccgggat gggctacgac accgtcctgc agcggatcat ccagtgcggc gagaactacg
     6481 acgcggcgtg gcgggaaagc tgagaggaaa gtggctagtg gctagtggtc agtggttagt
     6541 ggaagaaggc acggcaggct cacgctcttc cactagccac tagccactag ccactcgcca
     6601 cttcctcaga gggtgcttcc cgtccgcgag aagtgcgtcc tctcgtacac ggtctggcag
     6661 tcgatgcagc gcgcggcgtg cggcgtcgct tcgagccggc gcacctcgat cgggcggtcg
     6721 cacacgctgc aggtgccgta gctgccgtcg gcgacccgca gcagggccgc gtcgatgacg
     6781 cgcagttcct cgaggtctcg gtcgatctcg gcgaggctca cgtcgacgat caggtcggcg
     6841 aacgattcgt cctcgaggtc gcgcacgtcg tgggcgatct gcaggtactg ctcggagtcg
     6901 ctcttgagca gcgtctggcg gatctcttcg cgcagctggc gggcgcgttc gagcatcgcc
     6961 gcccgcagcg cttcgacctg gcggcggtcg agatcgtcga gcgtggtcgt ggcggtcgtc
     7021 atcgtgcttc ttccgtgagt gccgggcgag gcaagcatcg gctgccccgg ccaggcggtg
     7081 tatacgggga attgcacaac gccgctgcgg caagcgcggt ccgtccggtg tgaacacgca
     7141 gcggtctggg gcccggcgtc gccgctcccg tatgacggag acgcaagggc gccaagcgag
     7201 ggcggcgtca gtccggcggc aggttgcgcg cgccggggaa cagcacgtcc tcgaagccaa
     7261 ggttacgcag gtccgcgact cgcatggggt agaggatgcc gtcgaggtgg tcgacctcgt
     7321 gctggacgac gcgcgcgtgg aaccccgaca cggtgcggtc gatgggccgg ccctccgggt
     7381 cgtagccccg gtagcgcagg cggcggtatc gcggcacgag cccgcgcagg cccgggaccg
     7441 acagacagcc ttcccaggcg tcctcctgct cggtgccgag cggctcgagc gccggattga
     7501 cgagcaccgt gtacggcacg gtctccgcct gcggatagcg cgggttcgcc gtcacttcga
     7561 agatcacgac acgccacgac acgccgatct gcggcgcagc gatgcccgca ccgtcgagcg
     7621 aacgcatcgt gtcgtccatg tcccggacga gctcgcgcag ctcctcggtg ccgaagcgct
     7681 cgatgggttc ggcaacccgc tggagcaacg ggtgacccat gcggaggacc ggacggatcg
     7741 ccatgtggtg cggacctcgg cggcggaaac ctcgagcgta gacgagtcgg gcggattgac
     7801 aagggtggag cgccggcgta gctaggcgtc ccgtggacca cggactcagg tgctggaagt
     7861 gcggcggctc gctcgccgac tactcgctgc cgttgcgcag gatcgaggaa tgccgtgcct
     7921 gtcgcgccga gctgcacgtc tgccgcatgt gcgagttcta cgacacgtcc aaggccaagc
     7981 actgccgcga gacgatcgcg gacgaggtca aggacaagca gcgcgccaac ttctgcgatt
     8041 acttcaagcc gacgcccgcg gcatggcagc ccggcgcgac gaccgccgcc gacaaggctc
     8101 gcgcggagct tgaggcgctg ttcggtcgaa aatagcgcca tcgcgccggt ttgccggagc
     8161 ggccggcagc gcgacgggcc gtgtcaacag cggagtgcct ctcgcctgtg cctggcacga
     8221 tcgcgtgcga agcgaaggat tcggctggga ctggcgggcg gcttgaagag cagttccacc
     8281 agatggccca ggcggcgatg ggtgacgccg cgcttgagct gctcgaacca gcacagcagc
     8341 agccagcgct gctcgtaatc gtcgagcgcc gtgacgagcg cggaggagtg gccttggggg
     8401 acgctggccc gcagccgctg gctgccctcc agcgcatgcc gggcaatgcg cgagatctcg
     8461 gtgcgactcg ccgacgacgg cgtctgccag tagacgtagt ggctttccgg gtacagcagg
     8521 aagcgggcgc cttgaaacag cgcgtgcacg tagaggttga agtcctcggc gaacctgagg
     8581 tcttcggcat agcgcaactt gcgctcgtcg aggaaccgcc gccgcatcag gggcttgaga
     8641 tacccgaggc tgcagcgtcc tccgccggtg ccgctgcgga tcatggcctc cgcggacaga
     8701 cgttccaggt cgggcccgtc gcgctgccag gtcatgagct ccgccggagc gccgggcggc
     8761 cgcgcgcccc ggaaaaggat gccgtcggcg atgaggtcgg cctggtgact ctcccccgct
     8821 tcggcaagca ggcgcagacg atcgggc
//
LOCUS       DASPFR010000110         6308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_277769, whole genome shotgun sequence.
ACCESSION   DASPFR010000110 DASPFR010000000
VERSION     DASPFR010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277769"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2420
                     /gene="bamA"
                     /locus_tag="VD737_02765"
     CDS             <1..2420
                     /gene="bamA"
                     /locus_tag="VD737_02765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HXW09509.1"
                     /translation="SRPMRLKLMAAIPALLAVPAVLAQGDVGAFTVGDIRIEGLQRIT
                     EGTVYNYLPVNIGDRLDQRRIDEALRALYATGFFRDVELRRDGGTLVIAVLERPSIES
                     FTISGNKDIKTEDLEKSLRNVGLARGKTFNQSTLDEVTRYLTDQYYSRGKYAVKVDTR
                     VEEVPGNRVKIAIEVAEGKRSKIRQINIVGNEAFSDDELLEEFKLRTPNWLSWYRQDD
                     RYSREELSGDIEKLRSHYLDRGYANMNVESTQVAIAPEKDDIFITLNVREGDVYRVSD
                     VKIAGNLVVPEEQLRALLTVRRGDIFSRKQITTTTELMQLRLGQDGYAFAKIDPVPKE
                     NKETKEIELTFLVDPGNRAYVRHITFSGSTGINDDVLRREMRQMEGGYLSNAAIERSK
                     QRLRRLPYIEEVEVETNPVPGTPDLVDVDYQIKEGLPGQFSGGIGYSESQSILLNGSF
                     VHSNFMGTGNRVAAEINAGQYSKVFSLSHTDPYRTVDGVSRTLSATYRQLDQYTTSSS
                     DFETETGTLGVDYGWPISEFQSLRVGLAAQRSDLFTDPNSSAAEALYWVQNNGNTYTE
                     TSSIGIPPLTFTYYGTKFDTFELSGGWSFDSRNRAIFADRGARHRLSMNATVPGSDVE
                     YFSVHYDYLQFIPLGRLFTIMFNSEVSYGDSLGDTTSLPPYRRFFAGGPETVRGYRES
                     RLGPKDSFGNPYGGNLKFTNQLELLLPMPAKWRNSARFSAFVDAGNVFSTDDVQFVGK
                     DRVTPVDYDFSFDKLRYSTGVAVQWMAPLGVFRFSYAVPLNEFKGNDVEYKDETEGFQ
                     FSIGQAF"
     gene            2434..2967
                     /locus_tag="VD737_02770"
     CDS             2434..2967
                     /locus_tag="VD737_02770"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HXW09510.1"
                     /translation="MRTKTALLALVTIAASALPLGAAQAQAKIGVVNVARLLQESPQA
                     QAASQSLEGEFANRKRELQTMERDLKTREEKLTKDGATMAEAERRNQEKTLRDAQRDY
                     ARKQNEFMEDLNVRRNEALGQLQRTVLQEVQTYAKSAGLDLVVADALYASPTVDITQQ
                     VLSALQAKGKTAPPAKP"
     gene            2997..4058
                     /gene="lpxD"
                     /locus_tag="VD737_02775"
     CDS             2997..4058
                     /gene="lpxD"
                     /locus_tag="VD737_02775"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HXW09511.1"
                     /translation="MPVSLGELAVRFGSELHGDPDAAVDRVATLQDAGPGAVAFLANP
                     RYRKHLAGTRATVVVLDAASLGHCRTNALVATNPYATYARIARHLNPEPGFAGGRHPS
                     AVVEPDAIVDATAWVGPHCYVGAGAVIGPGVFLGPGTLVLAGVSVGRDSRCVARVTLC
                     ERVRIGDRCLLHPGVVIGADGFGHAPDQDGYVKVPQLGAVTVGDDVEVGANTTIDRGT
                     IDDTVIGHGVKIDNQVQIGHNCRVGEHTVIAGCAGISGSVTIGRRCMIGGMVGMAGHL
                     EVVDDVYLTGKTMVSTSITKPGLYSGKLPFDEARRFRRNSARFQKLDELAQRVRRLER
                     AQRPGGDASGNDDGDENDT"
     gene            4055..4504
                     /gene="fabZ"
                     /locus_tag="VD737_02780"
     CDS             4055..4504
                     /gene="fabZ"
                     /locus_tag="VD737_02780"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HXW09512.1"
                     /translation="MNDAQGGFGIDEVMRRLPHRYPMLLVDRVLECVSGERLVALKNV
                     SANEEFFAGHFPGRPVMPGVMMLEALAQAAGILCFVTANIYPDENVKFYFAGIDKARF
                     RRPVVPGDRLLLKVVLERRIRTIWKFSTVAEVDGDQACSAEMMVMPG"
     gene            4538..5317
                     /gene="lpxA"
                     /locus_tag="VD737_02785"
     CDS             4538..5317
                     /gene="lpxA"
                     /locus_tag="VD737_02785"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HXW09513.1"
                     /translation="MSGSIHATAIVAPDAVLGEGVEIGAYAVVGPGVEVGPRTRIGAH
                     ATVHCHTRLGADNLVHSYASVGDAPQDKKYGGEPTRLEVGSRNVIREFVTLNRGTTKD
                     RGVTKIGDDNLFMAYSHVAHDCVVGSQCVLANSATLGGHVVLGDWVIMGGFAAIHQFC
                     KVGAHAFLANNAAVTRDVPPYVMAVGGPAKPHSINSEGLKRRGFTPQQIRNLRTAYRV
                     LYRSGLRLEDALEQLRLMAVEQPEVQALVDFIPQSTRSLVR"
     gene            5397..>6308
                     /gene="lpxB"
                     /locus_tag="VD737_02790"
     CDS             5397..>6308
                     /gene="lpxB"
                     /locus_tag="VD737_02790"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HXW09514.1"
                     /translation="MLKIALVAGEASGDNLGGGLIRALAARAQDTRCFGVAGPRMAEA
                     GCEAWHSSDELAVMGLAEILKHLPRLLRLRRDLVSRLVAERPDVFVGIDAPEFNLRVA
                     RQLKAAGVPTVQYVSPQVWAWRQGRVRTIGQAVDLVLCVLPFERRFYDEHHVRAVFVG
                     HPLADRVPMESPPAPARLALGLAADRPTVAVLPGSRRAEVGKLGAPFAATLAWLRARR
                     PELQFVAPMANAAARAAFERALAAHAAGVDVTLVDGRAHEALAAADAVLVASGTATLE
                     TALVKRPMVVAYRVAPLTGWLLRDLKLV"
BASE COUNT         1050 a         2185 c         2152 g          921 t
ORIGIN      
        1 tgagccgccc catgcgactg aagctcatgg ccgcaattcc ggccctgctc gccgtacccg
       61 cggtgctcgc gcagggcgac gtcggcgcct tcacggtcgg cgacatccgc atcgagggcc
      121 tgcagcgcat caccgagggc acggtctaca actacctgcc cgtcaacatc ggcgaccgcc
      181 tcgaccagcg ccgcatcgac gaggcgctgc gcgcgctcta cgcgaccggc ttcttccgcg
      241 acgtcgagct gcgtcgcgac ggcggcacgc tcgtgatcgc cgtgctcgag cgaccttcga
      301 tcgagagctt cacgatcagc ggcaacaagg acatcaagac cgaggacctc gagaagtcgc
      361 tgcgcaacgt cgggctggcg cgcggcaaga ccttcaacca gtcgacgctc gacgaggtca
      421 cgcgctacct gaccgaccag tactactcgc gcggcaagta cgcagtgaag gtggacacca
      481 gggtagagga ggtgcccggc aaccgcgtca agatcgcgat cgaggtggcc gagggcaagc
      541 gctcgaagat ccgccagatc aacatcgtcg gcaacgaggc gttcagcgac gacgagctgc
      601 tcgaggagtt caagctgcgc acgccgaact ggctctcgtg gtaccggcag gacgaccgct
      661 attcgcgcga ggagctgtcg ggcgacatcg agaagctgcg ctcgcactac ctcgaccgcg
      721 ggtacgcgaa catgaacgtc gagtccaccc aggtcgcgat cgcgccggag aaggacgaca
      781 tcttcatcac gctcaacgtg cgcgaaggcg acgtctaccg cgtgtcggac gtcaagatcg
      841 cgggcaacct ggtcgtgccg gaggagcagc tgcgcgcgct gctcaccgtg cgccgcggcg
      901 acatcttctc gcgcaagcag atcacgacga cgaccgagct gatgcagctg cgcctcggcc
      961 aggacggcta cgcgttcgcg aagatcgacc cggtgccgaa ggaaaacaag gagaccaagg
     1021 agatcgagct cacgttcctg gtcgatccgg gcaaccgtgc ctacgtgcgc cacatcacgt
     1081 tcagcggcag caccggcatc aacgacgacg tgctgcgccg cgagatgcgc cagatggaag
     1141 gcggctacct ctcgaatgcc gccatcgagc gctccaagca gcgcctgcgc cggctgccct
     1201 acatcgagga ggtcgaggtc gagaccaatc cggtgccggg cacgccggac ctcgtcgacg
     1261 tcgactacca gatcaaggaa ggcctgccgg gacagttcag cggcggcatc ggttactccg
     1321 agtcgcagtc gatcctgctg aacggcagct tcgtgcacag caacttcatg ggcacgggca
     1381 accgcgtcgc cgccgagatc aacgcaggcc agtacagcaa ggtcttcagc ctgtcgcaca
     1441 ccgacccgta ccggacggtc gacggcgtgt cgcgcacgct ctcggcgacc tatcgccagc
     1501 tcgaccagta cacgacctcg tcctcggact tcgagaccga gacgggcacg ctcggcgtcg
     1561 actacggctg gccgatctcg gagttccagt cgctgcgcgt cggcctggcg gcacagcgct
     1621 ccgacctgtt caccgatccg aactcgagcg cggccgaagc gctgtactgg gtgcagaaca
     1681 acggcaacac ctacaccgag accagctcga tcggcatacc gccgctcacc ttcacgtact
     1741 acggcaccaa gttcgacacg ttcgagctgt cgggcggctg gtcgttcgac tcgcgcaacc
     1801 gcgcgatctt cgcggaccgc ggcgcccgcc accgcctgag catgaacgcg acggtcccgg
     1861 gcagcgacgt cgagtacttc tcggtgcact acgactacct gcagttcatc ccgctcggca
     1921 ggctgttcac gatcatgttc aattccgagg tgagctacgg cgactcgctc ggcgacacga
     1981 cctcgctgcc gccgtaccga cgcttcttcg ccggcggtcc ggagacggtg cgcggctatc
     2041 gcgagagccg cctcggcccc aaggattcct tcggcaatcc ctacggcggc aacctcaagt
     2101 tcacgaacca gctcgagctg ctgctgccga tgcccgccaa gtggcgcaac agcgcgcgtt
     2161 tcagcgcgtt cgtcgacgcc ggcaacgtgt tctccaccga cgacgtccag ttcgtcggca
     2221 aggaccgcgt gacgccggtg gattacgact tttcgtttga taaactgcgt tattcgaccg
     2281 gcgtcgccgt ccagtggatg gccccgctcg gcgtgttccg tttcagctac gccgtgcccc
     2341 tcaacgagtt caagggcaac gacgtggaat acaaggacga gacggaaggg ttccagttct
     2401 ccatcggcca ggctttctga ggtatcccgt tccatgcgca ccaagaccgc actgctcgcc
     2461 ctcgtgacca tcgccgccag cgcgctgccg ctcggcgcgg cccaggcaca ggcgaagatc
     2521 ggcgtcgtca acgtggcgcg cttgctgcag gaatcgccgc aggcccaggc ggcctcgcag
     2581 tcgctcgagg gcgagttcgc caaccgcaag cgcgagctgc agaccatgga gcgagacctg
     2641 aagacgcgcg aggagaagct caccaaggac ggcgccacga tggccgaggc cgagcgccgc
     2701 aaccaggaga agacgttgcg cgacgcgcag cgcgactacg cgcgcaagca gaacgagttc
     2761 atggaagacc tcaacgtccg ccgcaacgag gcgctcggcc agttgcagcg cacggtgctg
     2821 caggaggtcc agacctacgc caagagcgcg ggcctcgacc tcgtggtcgc cgacgcgctg
     2881 tacgcgagcc cgacggtcga catcacgcag caggtcctct cggcgctgca ggccaagggc
     2941 aagacggctc cgccggccaa gccctgagtc aagagcgagc ggcgcaggcg tccgccatgc
     3001 cggtgagcct gggcgagctg gcggtacgct tcggaagcga gctgcacggt gacccggacg
     3061 ccgccgtcga ccgggtcgcg accctgcagg acgccgggcc gggcgcggtc gcgttcctcg
     3121 ccaatcccag gtatcgcaag cacctcgccg gcacgcgggc gaccgtcgtc gtgctcgacg
     3181 cggcgagcct gggccactgc cgcaccaacg cgctcgtcgc gaccaacccc tacgccacgt
     3241 acgcgcggat cgcccgacac ctgaacccgg agcccggttt tgccggtggc cggcatccgt
     3301 cggccgtcgt cgagccggac gcgatcgtgg acgccacggc ctgggtcgga ccgcactgct
     3361 acgtcggtgc gggcgcggtc atcggccccg gcgtcttcct cgggccgggc acgctggtgc
     3421 tcgccggggt ctcggtgggc cgggacagcc gctgcgtcgc gcgggtcacg ctctgcgagc
     3481 gcgtgcggat cggcgatcgt tgcctgctgc acccgggcgt cgtgatcggg gcggacgggt
     3541 tcgggcacgc ccccgaccag gacggctacg tcaaggttcc acagctcggc gcggtcacgg
     3601 tcggcgacga cgtcgaagtc ggcgcgaaca ccacgatcga ccgcggcacg atcgacgaca
     3661 ccgtgatcgg ccacggcgtc aagatcgaca accaggtgca gatcggacac aactgccgtg
     3721 tcggggagca cacggtgatc gcgggctgtg ctggaatctc gggcagcgtg acgatcggcc
     3781 ggcgctgcat gatcggcggc atggtcggca tggccgggca cctcgaggtg gtcgacgacg
     3841 tctacctgac cggcaagacg atggtcagca cctcgatcac gaagcccggg ctgtattcgg
     3901 ggaagctgcc gttcgacgag gcgcgcaggt tccggcgcaa cagcgcgcgg ttccagaagc
     3961 tcgacgaact ggcccagcgc gtgcgtcggc tcgagcgcgc gcagcgtccc gggggggacg
     4021 cgtccggcaa cgacgacggc gacgagaacg acacatgaac gacgcacagg gtggattcgg
     4081 catcgacgag gtgatgcgga ggctgccgca tcgttatccg atgctgctcg tggatcgcgt
     4141 gctcgagtgc gtctcgggcg agcgactggt cgcgctcaag aacgtgagcg ccaacgagga
     4201 gttcttcgcg gggcacttcc ccggcaggcc ggtcatgccc ggggtgatga tgctcgaagc
     4261 gctcgcgcag gccgccggca tcctctgctt cgtcacggcc aacatctacc cggacgagaa
     4321 cgtgaagttc tacttcgcgg gcatcgacaa ggcgcgcttt cgccggcccg tcgtgcccgg
     4381 cgaccgcctg ctgctcaagg tcgtgctcga gcggcggatc cgcacgatct ggaagttctc
     4441 gacggtcgcc gaagtcgacg gcgaccaggc ctgttcggcc gagatgatgg tgatgccggg
     4501 ctgaggccgc ggcgaaaggg gagggagagc aaacgagatg agcggcagca tccacgcgac
     4561 tgcgatcgtc gcgcccgacg ccgtgctcgg cgaaggcgtc gagatcgggg cgtacgccgt
     4621 cgtcgggccg ggcgtcgagg tcggtccgcg cacgcggatc ggcgctcacg cgaccgtcca
     4681 ctgccacacg cggctcgggg cagacaacct cgtgcactcg tacgcgtcgg tcggcgatgc
     4741 gccgcaggac aagaagtacg ggggcgagcc gacgcggctc gaggtcggca gtcgcaacgt
     4801 gatccgcgag ttcgtcacgc tgaaccgcgg cacgaccaag gaccgcggcg tcacgaagat
     4861 cggcgacgac aacctgttca tggcctattc gcacgtcgcc cacgactgtg tcgtgggatc
     4921 acagtgcgtg ctcgccaaca gcgccacgct cggcggccac gtggtgctcg gcgactgggt
     4981 catcatgggt ggcttcgcgg cgatccacca gttctgcaag gtcggcgcgc acgcgttcct
     5041 cgccaacaac gcggccgtca cccgcgacgt gccgccgtac gtgatggcgg tcggcgggcc
     5101 ggccaagccg cacagcatca attccgaggg gctcaagcgg cgcggcttca cgccgcagca
     5161 gatccgcaac ctgcgcacgg cgtaccgcgt gctgtaccgg tcggggctgc gtctcgagga
     5221 cgcgctcgaa cagctgcggc taatggccgt ggagcagccg gaggtccagg cgctcgtcga
     5281 cttcattccg caatccacgc gcagcctcgt gcggtgaggc cgtgggcggg gatccggaat
     5341 tcacctctcc cgcgccagtc ggggaggctc tagccgctcc ccgcgcgtcg ggagaggtgc
     5401 tgaaaatcgc gctcgtcgcc ggcgaagcct ccggtgacaa cctgggcggc ggcctgatcc
     5461 gcgctctcgc ggcgcgcgcg caagacacac gctgtttcgg cgtcgcgggc ccgcgcatgg
     5521 cggaggcggg ctgcgaagcc tggcattcgt ccgacgagct cgccgtcatg gggctcgccg
     5581 agatcctcaa acatttgccg cgcctgctgc ggctccgccg cgacctggtg tcgcggctgg
     5641 tcgccgagcg ccccgacgtg ttcgtcggca tcgatgctcc cgagttcaac ctgcgcgtcg
     5701 cgaggcagct caaggccgcg ggcgtgccga ccgtgcagta cgtcagcccg caggtctggg
     5761 cgtggcggca gggccgcgtg cgcacgatcg gccaggccgt ggacctcgtg ctgtgcgtgc
     5821 tgccgttcga gcgccgcttc tacgacgagc accacgtgcg cgcggtgttc gtgggccatc
     5881 cgctggccga ccgcgtgccg atggagtcgc cgccggcgcc cgcgcggctc gcgctgggcc
     5941 tggcggccga tcggcccacg gtcgcggtcc tgcccggcag tcgtcgcgcc gaggtcggca
     6001 agctcggtgc accgttcgcg gcgacgctcg cgtggctccg ggcgcggcgc cccgagttgc
     6061 agttcgtcgc cccgatggcc aacgcggctg cccgagccgc gttcgagcgc gcactcgccg
     6121 cgcacgcggc gggcgtggac gtcacgctcg tggacgggcg tgcacacgaa gcgctcgccg
     6181 ccgcggatgc cgtgctcgtc gcctcgggca ccgccactct cgagacggcg ctcgtcaagc
     6241 gcccgatggt cgtcgcgtac cgcgtcgcgc ccctcacggg ctggctgctc agggacctga
     6301 agctcgtc
//
LOCUS       DASPFR010000111         6289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1741862, whole genome shotgun sequence.
ACCESSION   DASPFR010000111 DASPFR010000000
VERSION     DASPFR010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1741862"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="VD737_02795"
     CDS             <1..1019
                     /locus_tag="VD737_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09515.1"
                     /translation="FCRQVMIGYDTAETTGQQLADALRGCHERRLANPTSTDSATAQD
                     GGGASPDGTAVSAAEASAGTTFNLPVIGSVDAKALSLPVLTLVLAGMDAFNPCAFFVL
                     LFLLSLLVHAKSRARMAIVGGTFVLFSGIVYFVFMAAWLNVFLIAGELRVITFIAGLV
                     ALLVAVLNIKDYFWFKAGPSLSIPDSAKPGLFKRMREVVTEGSMGPMLVGTVLLAIVA
                     NSYELLCTAGFPMVYTLTLTKAHELATWQYYAWLAVYNVIYVIPLLAIVVVFTKTMGA
                     RKLTESEGRVLKLISGFMMLGFALVLLIAPAALTNALVSIGVLAVAVIASIVIARVSA
                     PRTA"
     gene            1101..2543
                     /locus_tag="VD737_02800"
     CDS             1101..2543
                     /locus_tag="VD737_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXW09516.1"
                     /translation="MSSAVTAPVRLRVVEHLRAVLDEDGALQAPGDVEKYLTDFRGLY
                     RGAALLVACPRDVTQVADVLRTCHDAGVGVVPHGGNTGYCGGATPDESGSQVVLSLHR
                     LNRIRAVDAANYSMTAEAGCVLADVQSAALEAGRLFPLSLGSEGSCQIGGNLSTNAGG
                     LAAVRYGVARDLVLGLEVVLANGDVLHGLSSLRKDNTGYDLRDLFIGAEGTLGVITAA
                     SLKLFPVPRTVETAFLAVPGMQAAVGVLAGLREATGDCVTSFEYLPRIAVEFTTRHVP
                     GVVDPLGDVHPAYVLCEVSTARDDPQLRPLLESALQRCMDAGLVLDATLAASNAQRAA
                     LWKLRESVPEAQKHEGASIKHDVSVPVASLPEFLERASAAVTRVAPRARLVAYGHVGD
                     GNLHFNLSEPAGGQERARFLAQQDAINDAVHECVRSFGGSISAEHGIGRLKRALLPRY
                     KDPVALATMRALKRTLDPNGILNPGKLLPD"
     gene            2545..5658
                     /locus_tag="VD737_02805"
     CDS             2545..5658
                     /locus_tag="VD737_02805"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HXW09517.1"
                     /translation="MHALLRSKITWTLAALALLVGSYAALGFYAAPLLVRSQAIEYVK
                     ETHGRELTLGDVHIHPFKLHVELRDLSLPDADGTPMIAFRRLFVDFEALSSLWERAFV
                     FREIALDGPRVRTVIRPDGSLNLADLAAAQDQHEDQDDAPPAVWVQSLTLTGGEINLF
                     NQLRRRPVERHFAPVTFTLRDFRTTAEGGRFGLAARSRQDEEFEWKGVFALAPRIVSN
                     GEFAIRHLRAPGVAEFLGDDLPFLVPTGSIDLAGTYTLDVGDRLALGLNLPTIRASDL
                     TLRARGEDQDWIRIPSIVVSNTTSTVPALATAMGDVVIEGLEARLWRDADGALNLQRL
                     FETSPVGTDGQAGSAGALPPGPSSTPPAAPASGAPAGEPTLDLGTLHLRHARIDFEDR
                     GVQPAVRVALAPLEFTTRDVSLDLSRPLPVDLDATINGRARLAASGTFVPETSALDVG
                     VELAGLAVKDLQPYAAGGTDLSIERGRLDASGRFSLSPPGGAKPEVGFEGDLAIVGFR
                     SKDSALEQDFLDFERVELSKLRFTMAPDALNIDRVRVVRPFTRVIVSSDQVINVAAVF
                     DPEGTATALAARKAEAAAKASQAVRKKSRAELRAEKRAAAEAAKARAKAPPPAPDLRE
                     TGMPIRIREVEVVGGTLDFSDYSVQPNFAAAIQALQGKVTGLSSDPRSRATIALAGNV
                     GEFSPVRIEGTTQPFAFDRYTDVGFKFENISLPVFNPYSGKFAGYSIAKGKLTTDLHY
                     RIDARQLEAQHRIRIDQLEWGEATAARGEATLPVKFATSLLKDADGVITLDVPVSGTL
                     DDPTFRIGPIVWQIIKNVLSKAVTAPFRALGALFKGAEEAQFVEFAAGQAALESVAAE
                     RLGALGRSLAGKPDLRLDVPIGIDATVDGAALAQARYAQALADATTAVLAGRRRGDNP
                     PPPPPAFDTLEPERQREVLERLYRDLSGAEPQLPEPPELPDDLSRQERRARELQASLD
                     WLEAESRKRALPLPGELERLGQQRGEAIEAALLADTGMAAERVFLTRTGKVTAEASRV
                     RFELEVK"
     gene            complement(5707..>6289)
                     /locus_tag="VD737_02810"
     CDS             complement(5707..>6289)
                     /locus_tag="VD737_02810"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HXW09518.1"
                     /translation="GQQIFHLSASFQVEEPGPDHQVAAPNVTPPEQLPDLATTMRRRL
                     DEMPEQRRRFWERDRPFEFRQVEEIATGAPQPGVRHVWFRLAGPCQGDDTLHRCLLAY
                     VSDYNLLETATLPHGISYHRDDVLLASLDHGLWFHRPFRIDAWLLYATISPSTSGARG
                     MAFGRIFDRSGRLVASTAQEGLMRVLRKGGHSE"
BASE COUNT          919 a         2188 c         2209 g          973 t
ORIGIN      
        1 tgttctgccg gcaggtcatg atcggttacg acaccgccga gaccaccggt cagcagcttg
       61 cggacgctct gcgcggctgc catgaacgcc ggctcgcgaa cccgacgtcg accgactccg
      121 cgacggcgca ggacggcggc ggcgcctccc cggacggcac ggccgtgtcc gccgccgagg
      181 cgagcgcggg cacgaccttc aacctgcccg tgatcggctc ggtggatgcc aaggcgctgt
      241 cgctgcccgt gctcacgctc gtcctcgcgg gcatggacgc gttcaacccc tgcgcgttct
      301 tcgtgctgct gttcctgctg agcctgctgg tgcacgccaa gagccgggcg cgcatggcga
      361 ttgtcggcgg cacgttcgtg ctgttctcgg gcatcgtcta cttcgtcttc atggccgcgt
      421 ggctcaacgt gttcctgatc gcgggcgagc tgcgcgtgat cacgttcatc gcgggcctgg
      481 tcgcgctgct ggtcgccgtg ctcaacataa aggactactt ctggttcaag gccggcccga
      541 gcctgtcgat ccccgactcg gcgaagccgg ggctcttcaa gcggatgcgc gaggtcgtga
      601 ccgagggcag catgggaccg atgctggtcg gcacggtgct gctcgcgatc gtcgcgaact
      661 cgtacgaatt gctttgcacc gccggcttcc cgatggtcta cacgctcacg ctgaccaagg
      721 cacacgagct cgcgacgtgg cagtactacg cgtggctcgc ggtctacaac gtcatctacg
      781 tgattccgct gctcgcgatc gtggtcgtat tcacgaagac catgggcgcg cgcaagctga
      841 ctgaatccga aggccgcgtg ctcaagctga tctcgggatt catgatgctc ggcttcgcgc
      901 tggtgctgct gatcgcaccc gccgcgctca ccaacgcgct cgtgtcgatc ggcgtgctcg
      961 ccgtggccgt gatcgcgtcg atcgtgatcg ccagggtgag cgcacccagg acggcctgac
     1021 cggggccaac ctcgtgcgcg gccgcacgcg cgcgccgcgc tggcaatcgc ctccgtagcc
     1081 tgcgttacac tcgcgggacg atgagttccg ccgtcaccgc ccccgtccgg ctccgggtcg
     1141 tggagcacct gcgcgccgtg ctcgatgagg acggtgcgct tcaggcgccg ggcgacgtcg
     1201 agaagtacct gacggatttt cgcggactct accggggggc ggcgctgctg gtcgcctgcc
     1261 cgcgcgacgt cacgcaggtg gcggacgtcc tgcggacctg ccacgacgcg ggcgtcggcg
     1321 tggtgcctca cggcggcaac accggttact gcggcggcgc gacccccgac gagtcgggca
     1381 gccaggtcgt gctgtcgctg caccggctga accgcatccg cgcggtcgac gcggcgaact
     1441 attcgatgac cgccgaggcg ggctgcgtgc ttgccgacgt gcagtcggcg gccctcgagg
     1501 ccgggcgcct ttttccgctg tcgctcggtt cggaagggag ctgccagatc ggcggcaacc
     1561 tttcgacgaa cgccgggggc ctcgctgccg tgcggtacgg cgtcgcccgc gacctcgtgc
     1621 tcgggctcga ggtcgtgctc gcgaacggcg acgtgctgca cggcctgagc agccttcgca
     1681 aggacaacac cgggtacgac ctgcgcgacc tgttcatcgg cgccgaaggc acgctgggtg
     1741 tgatcactgc ggccagcctg aagctgtttc cggttccgcg caccgtcgag accgcgttcc
     1801 tcgcggtgcc gggcatgcag gccgccgtcg gcgtgctcgc cggacttcgt gaggcgacgg
     1861 gcgactgcgt cacgagcttc gagtacctcc cgcggattgc ggtcgagttc acgacgcggc
     1921 acgtgccggg ggtcgtggac ccgctcggcg acgtgcatcc agcctacgtg ctttgcgagg
     1981 tgtcgacggc gcgggacgat ccgcagctgc ggccgctgct cgaatcggcg ctgcaacgct
     2041 gcatggacgc cggactggtc ctggacgcga cgctggccgc gtcgaacgcc cagcgcgcgg
     2101 cgctctggaa gctgcgcgaa tcggtgcccg aggcgcagaa gcacgaaggt gccagcatca
     2161 agcacgacgt ctcggtgccg gtggcgtcgc tgcccgagtt cctcgagcgg gcgtctgcag
     2221 cggtcacgcg cgtggcgccc cgcgcacgcc tggtcgccta cgggcacgtc ggcgacggca
     2281 acctgcactt caacctgagc gagcccgcgg gcggccagga gcgcgcgcgc ttcctggcac
     2341 aacaggacgc gatcaacgac gccgtgcacg agtgcgtccg ctcgttcggc ggcagcatca
     2401 gcgccgagca cggcatcggt cggctcaagc gcgcgttgct gccccgctac aaggacccgg
     2461 tggcgctcgc gacgatgcgc gcgctcaagc gcacgctcga cccgaatggc atcctgaatc
     2521 caggcaagct gctgcccgac tgacatgcac gcgctgctgc gatctaagat cacgtggacg
     2581 cttgcagcgc ttgcgctgct cgtggggtcg tacgcggcgc tcggcttcta cgccgccccg
     2641 ctcctggtgc gcagccaggc gatcgagtac gtgaaagaga cgcatgggcg cgagctcacg
     2701 ctcggcgacg tgcacatcca tcccttcaag ctgcacgtgg agctgcggga cctgtcgctg
     2761 ccggacgcgg acggcacgcc gatgatcgcg ttccggcggt tgttcgtcga cttcgaggcg
     2821 ctgtcgtcgc tgtgggagcg cgcgttcgtg tttcgcgaga tcgcgctcga cggccctcgc
     2881 gtgcggaccg tgatccggcc cgacggctcg ctcaacctcg cggatctggc ggcggcgcag
     2941 gatcagcacg aggatcagga cgacgcaccg ccggccgtct gggtccagtc actgacgctg
     3001 accggaggcg agatcaacct gttcaaccag ttgcggcggc ggcccgtcga aaggcatttc
     3061 gcgccagtga ctttcacgct gcgcgacttc cgcacgaccg cggaaggcgg tcgcttcggt
     3121 ttggcggcgc gcagccgcca ggacgaggag ttcgagtgga agggcgtttt cgcgctcgcg
     3181 ccgcggatcg tctcgaatgg agagtttgcg atccgccacc tgcgcgcgcc cggcgtggcg
     3241 gagttcctcg gcgacgacct gcctttcctg gtgccgaccg gcagcatcga cctcgccggc
     3301 acgtacacgc tcgacgtggg cgaccgcctg gcgctcggct tgaacctgcc gacgatccgg
     3361 gcgtcggacc tgaccctgcg cgcgcgcggc gaggaccagg actggatccg catcccgtcg
     3421 atcgtcgtct cgaacacgac gtcgaccgta ccggcactgg cgacggcgat gggcgacgtc
     3481 gtgatcgagg gcctcgaggc gcggctctgg cgcgatgcgg acggcgcgct gaacctgcag
     3541 cggttgttcg agacgagccc cgttggcacc gatggtcagg caggttccgc aggcgccctg
     3601 ccacctgggc cgtcgagcac acccccggca gcgcctgcca gcggcgcgcc ggctggcgag
     3661 ccgacgctgg acctcggcac gttgcatctg cgtcacgcca ggatcgactt tgaggaccgc
     3721 ggcgtgcagc ctgcggtgcg cgtcgcgctc gcaccgctcg agttcacgac gcgcgacgtg
     3781 agcctggacc tgtcacggcc gctgccggtg gacctggacg ccacgatcaa cggacgcgcg
     3841 cgactggccg cctcgggcac gttcgtgccc gagacgtccg cgctggacgt gggcgtcgag
     3901 ttggcgggcc tggcggtcaa ggacctgcag ccgtatgcgg cgggtggcac cgacctctcg
     3961 atcgaacgcg gccggctcga cgcgagcggg cgattctcgc tgtcgccccc cgggggcgcc
     4021 aagcccgaag tcggcttcga aggcgacctc gcgatcgtcg gcttccgctc caaggacagc
     4081 gcgctcgagc aggacttcct cgacttcgag cgcgtggagc tgtcgaaact gcgcttcacg
     4141 atggcgccgg acgcgctcaa catcgaccgc gtccgcgtcg tccggccgtt cacccgcgtg
     4201 atcgtgagct ccgaccaggt gatcaacgtc gcggcggtct tcgaccccga gggcacggcg
     4261 acggcgctcg ccgcccgcaa ggccgaggcc gccgcgaagg cgtcgcaggc ggtgcgcaag
     4321 aaatcacgcg ccgagctgcg cgccgagaag cgcgcggcgg ccgaggccgc gaaagcgcgc
     4381 gccaaggcgc ccccgccggc acccgacctg cgcgagacgg gcatgccgat ccggatccgc
     4441 gaagtcgagg tcgtgggcgg cacgctggac ttctccgatt acagcgtgca gccgaacttc
     4501 gccgcagcga tccaggcgct gcaggggaag gtgacgggac tgtcgagcga tccgcgctcg
     4561 cgcgcgacga tcgcgctcgc aggcaacgtc ggcgagttct cgccggtgcg catcgagggc
     4621 acgacgcagc cgttcgcgtt cgaccgctac acggacgtcg gcttcaagtt cgagaacatc
     4681 tcgctgccgg tcttcaatcc gtactcgggc aagttcgccg gctacagcat cgccaagggc
     4741 aagctcacga ccgaccttca ctacaggatc gatgcgcggc agctcgaggc gcagcacagg
     4801 atccgcatcg accagctcga gtggggcgag gcgaccgcgg cgcgcggcga ggccacgctg
     4861 ccggtcaagt tcgcgacgtc gctgctcaag gacgccgacg gcgtcatcac gctggacgtg
     4921 ccggtctccg gcacgctgga cgatccgacg ttccggatcg ggccgatcgt ctggcagatc
     4981 atcaagaacg tgctgagcaa ggccgtgacg gcgccgttcc gtgcgctcgg cgcactgttc
     5041 aagggtgccg aagaagctca gttcgtcgag ttcgccgcgg gccaggccgc gctcgaatcg
     5101 gtggccgcgg aacggctcgg agctctcggc cgcagcctcg cgggcaaacc cgacctgcgg
     5161 ctcgacgtgc cgatcggcat cgacgccacg gtggacgggg cggcgctcgc gcaggcgcgt
     5221 tacgcgcagg cgctggcaga cgcgaccacg gccgtgctgg ccggtcgccg ccgtggcgac
     5281 aatccgcctc caccgccgcc cgcgttcgac acgctcgaac ccgagcgcca gcgcgaagtg
     5341 ctggagcggc tgtatcgcga cctttcgggc gccgagccgc agctaccgga accgccggag
     5401 ctccccgacg acctgtcgcg ccaggaacgc cgtgcgcggg agttgcaggc gtcgctcgac
     5461 tggctcgaag ccgagtcgcg caagcgtgca ttgccgttgc cgggagaact cgagcggctc
     5521 ggccagcagc gtggcgaggc catcgaggcc gcgctgctcg cggacaccgg catggccgcc
     5581 gagcgcgtct tcctgacgcg caccggcaag gtcaccgccg aggcgtcacg ggtgcgcttc
     5641 gaactcgagg tgaaataggg acactccgaa tatccgcgaa attcggagtg tccccattta
     5701 cggtgattat tcggagtgtc ccccttttcg gaggacgcgc atcaggcctt cctgcgcggt
     5761 gctcgccacg agccggcccg agcggtcgaa gatgcggcca aacgccatgc cgcgcgcacc
     5821 gctcgtgctg ggactgatcg tcgcgtacag cagccacgcg tcgatccgga acgggcggtg
     5881 gaaccagagc ccgtggtcga ggctcgcgag cagcacgtcg tctcggtgat acgagatgcc
     5941 atgcggcagc gtcgcggtct cgagcaggtt gtaatcggag acgtacgcga gcaggcagcg
     6001 gtgcagcgtg tcgtcgccct ggcacgggcc cgccagccgg aaccagacgt ggcgcacgcc
     6061 cggctgcggc gccccggtcg cgatttcctc gacctggcgg aactcgaacg ggcggtcgcg
     6121 ctcccagaag cgccgccgct gctcgggcat ctcgtcgagc cgccgccgca tcgtcgtcgc
     6181 gaggtccggc aactgctccg gcggcgtgac gttcggcgcg gccacctggt ggtccggccc
     6241 gggctcctcc acctggaacg acgccgacag gtggaagatc tgctgcccg
//
LOCUS       DASPFR010000112         8357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_33675, whole genome shotgun sequence.
ACCESSION   DASPFR010000112 DASPFR010000000
VERSION     DASPFR010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8357
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33675"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VD737_02815"
     CDS             complement(<1..844)
                     /locus_tag="VD737_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HXW09519.1"
                     /translation="MLAVLNVLGSLLMLFSATYALPMITSLIYRDRLLVDFIYAAVLC
                     LGIGAIVYGATLRHRRELRSRDGFLLVTLAWIFMSAIATVPLLLALPGLSFTDAFFET
                     MSGLSTTGSTVLTGLDSMAPSLNIWRAALHWYGGMGIIVLAVAVLPLLGVGGMQLYKA
                     ETPGAVKNEKLTPRITQTAKALWVVYVLITIACILALKTAGMSWFDAVFHAFSTVALG
                     GFSSYDSSIAHFDSLAVELVIIFFMLLAAMNFSRHFIAWQQKSLRTYVTDFEAIAMLR
                     LVGLS"
     gene            complement(872..2287)
                     /gene="trkA"
                     /locus_tag="VD737_02820"
     CDS             complement(872..2287)
                     /gene="trkA"
                     /locus_tag="VD737_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HXW09520.1"
                     /translation="MKIIILGAGQVGRTAAYHLAREEANDVTVVDLDEALLRDLQDRI
                     DIRTVQGNAASPRTLETAGARDADMIVALTNSDETNMVACHVAWSLFGTGTKIARIRS
                     RDYTRHDGLFVTTDAAPGDARATRAANERTPYGFAVDMYLSPEQAVTEYIERLIRYPG
                     ALQIVDFAAGKVRLVGLKALRGGALVGRPIRELKTHMRNSEARVAAMYRSGQSIEPEG
                     DTDILDGDEVFFVAATQDIRVVMREMQKLEDPVKRVVIAGGGNIGFRLAQSLEDENQV
                     KVIERDSKRARRISEQLRNTIVLVGDAADEELLVEENIDSVDVFCALTNSEEANILSA
                     MLAKRLGARRVMALISRPAYADLTQIGSIDVAISPQTVTIGSLLAYVRRGDVVQVHSL
                     RRGSAEAMETIAHGVRGGKVVGRPIEDIKLPEGAKIVTIVRGDRVVMAHHDTVIENGD
                     HVILFLSDKRHVEQVERLFQT"
     gene            complement(2372..4582)
                     /gene="uvrD"
                     /locus_tag="VD737_02825"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(2372..4582)
                     /gene="uvrD"
                     /locus_tag="VD737_02825"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HXW09521.1"
                     /translation="MDVSHILAPLNDAQRQAVTAPLKPTLVLAGAGSGKTRVLTHRVE
                     WLVQVEGVSPHGILAVTFTNKAAAEMRHRIEQQLGLPSAPLWIGTFHGICHRLLRMHW
                     REAGLPQGFQILDGEDQQRLVKKVIRGLDLDDTRWVPREVSYFINARKDEGERPKHLR
                     DDGDPTRRQMIRLYEAYEEACRRNGVVDFAELLLRSYELLKDVPALGDHYRARFRHVL
                     VDEFQDTNTIQYAWMRVLVGDLSVPYVVGDDDQSIYRWRGARVENLQQYRKDFRDVQL
                     FRLEQNYRSTGNILDAANAIIGNNSGRIGKKLWTSEGRGAPIRLFRAWNERDEAEFVV
                     NKIREWVARGGQRRDNAILYRSNAQSRVFEEYLLAARIPYRVYGGLRFFERQEIKDAL
                     AYLRLIANRDDDASFERVVNLPTRGIGARTLEVLRAHAREHSTSMWRAAFECNDELGS
                     KAASCLQAFLALIEKLDAATQGLPLHEQVDHVIQASGLAAHYQKEKADKGEARLENLE
                     ELVSAARGFEPEDGDMTPLMAFLSHAVLESGEGQAEAWEDCVQMMTLHSAKGLEFPVV
                     FLCGLEDGLFPHQRSVTDTHGLEEERRLCYVGCTRAMRQLYLTYAEQRRMHGVDSYGA
                     PSRFIAEIPAPLVEEVRPKVQVSRPAYVPQSRVAGGYQAARPTGGNLSMSGKRFQDEA
                     PGGLRLGQRVRHAKFGDGVVLNLEGQGANARVQVNFERQGTKWLMMGYAHLEVV"
     gene            4728..6455
                     /locus_tag="VD737_02830"
     CDS             4728..6455
                     /locus_tag="VD737_02830"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HXW09522.1"
                     /translation="MTMRKPSFSRLCAHSCLAVLAGLAPMGASLADAASADAAWRVHV
                     ERFVESHLEANPVEAVYAGRHEFDGRLPDLSAAGIARNVARLEAERAKTAGFDPAKLS
                     EPSRREREYLLAALADELFWIRDAEWPFRNPSWYLGVVDPEVYLSRPYAPLETRLRAY
                     LDYARAIPAATEQIRANLRTPMPAPWVEYGVNAFGGFAQFFANDVGPVFADVEDDALQ
                     SQLEAANAAAARAMQALADWFVAQRPKATQDYALGAGMFAKMLEATEQVTVPVERLRE
                     IGEADLGRHLVALRKTCASYAPGATLRECADKANAHKPEGGAVAGARRQLPELKQFVV
                     QKNLAAIPGTEQALVEEAPPYNAQNFAYIQVPGPYEKTAVPAVYYIAPPDPRWTPEEQ
                     AQYVSGEGTLLSTSVHEVWPGHFLHFMHVNRSTSKIAQLFQSYAYTEGWAHYTEEMMA
                     DEGLRAGDPEWHIGMRLSALKRNARYLCAIGLHTAGMTVAQCERLFRESALTDPGNAR
                     QQALRGTYDPAYLNYTLGKLMILQLREDWRAAHPKAPLRDFHDQFLGYSGPVPLVRRL
                     MLGTVSPPL"
     gene            complement(6582..>8357)
                     /locus_tag="VD737_02835"
     CDS             complement(6582..>8357)
                     /locus_tag="VD737_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HXW09523.1"
                     /translation="DMYVDQLAPAPGTPSGLVSVHQDGTGAVTRIPLTFKANLRDGLG
                     NGADTVVTIPATPQNRLPADVLTLSRRNQGPVVASLGSGRVVTVQYTGFGPTRELDTF
                     RLLNRARDLTDFTTALRYFDAGSQNFIYGDIEGNIGYFTTGEVPLREDLQANTVRGAP
                     PWFVRDGFGGNEWLKDPSPDAFNGTGYLSLPFEELPQTVNPSAGWVVNANNDTSGATL
                     DNDPLNQLRIGGQGIFYLGYAFDHGTRAGRITQALKERLVRGSVDRSDMQAIQADVTL
                     LDAQVLTPYITGAFLRAASAGAPAELAALAADPRVVEAVDRLGAWNHTTPTGVATGYD
                     AVDVNGQLSAPSSQEIARSVAATIYSVWRGQAINNAVDRTLDDLGVPRPGSGEAIKAL
                     RHLVERNGIGLSTVDFFAWTGLATPEQRRDYVMLRSLRDALDLLAGPAFEKAFARSTN
                     QDDYRWGKLHRIVFDGLVVGGPYSIPNAQLGFPPSFANLAGLATDGGFGVVDASSHGA
                     RAASADAFMFGSGPNRRYVGSPGTAPGSIEAETSLPGGMSGVLTSPFYANLLGRWLTN
                     DTYPLRTNRGEVLRSISSQQLFAPM"
BASE COUNT         1272 a         2837 c         2900 g         1348 t
ORIGIN      
        1 ccgagaggcc gaccagccgc agcatcgcga tggcctcgaa gtccgtgacg tacgtccgca
       61 ggctcttctg ctgccacgcg atgaagtgcc gcgagaagtt catggccgcg agcagcatga
      121 agaagatgat cacgagctcg accgcgagcg aatcgaaatg cgcgatgctc gagtcgtagc
      181 tcgaaaagcc gcccagcgcc acggtcgaga acgcgtggaa caccgcgtcg aaccagctca
      241 tgccggccgt cttgagggcg aggatgcagg cgatcgtgat caggacgtag accacccaca
      301 gcgccttggc ggtctgggtg atccgcggcg tcagcttttc gttcttgacc gcgcccggcg
      361 tctcggcctt gtagagctgc atgccgccca cgccgagcag cggcagcacg gcgacggcga
      421 gcacgatgat gcccatgcct ccgtaccagt gcagcgcggc ccgccagatg ttcagtgacg
      481 gcgccatgga gtcgagtccg gtcagcaccg tcgatccggt cgtgctcaag cccgacatgg
      541 tctcgaagaa agcgtcggtg aagctgagcc cgggcagcgc cagcagcagc ggcacggtcg
      601 cgatggccga catgaatatc caggccagcg tcaccagcag gaacccgtcg cgcgagcgca
      661 gctcgcgccg gtgcctgagc gtcgcgccgt acacgatcgc gccgatgccg aggcagagca
      721 ccgcggcgta gatgaagtcc accagcaggc ggtcgcgata gatcagcgac gtgatcatcg
      781 gcagcgcgta cgtggcgctg aacagcatca gcagcgagcc gaggacgttg aggacggcta
      841 gcaagggttc cgcctggcga agccggtggc gtcaggtctg gaacaggcgc tcgacctgct
      901 ccacgtgccg cttgtccgac aggaacagga tcacgtggtc gccgttctcg atgaccgtgt
      961 cgtggtgagc catgaccacg cggtcgccgc gcacgatggt cacgatcttg gcgccctccg
     1021 gcagcttgat gtcctcgatc ggccgaccca cgaccttgcc gccgcgcacg ccgtgcgcga
     1081 tcgtctccat cgcctccgcc gaaccccgtc gcagcgagtg cacctgcacg acgtcgccgc
     1141 gccggacgta ggccagcaac gagccgatcg tcaccgtctg cggtgagatc gccacgtcga
     1201 tcgagccgat ctgcgtcagg tccgcgtacg ccggccggct gatcagcgcc atcacgcggc
     1261 gggcgccgag ccgcttcgcg agcatcgcgg acaggatgtt cgcttcctcg gagttggtga
     1321 gcgcgcagaa cacgtcgacg ctgtcgatgt tctcctcgac caggagttcc tcgtcggcgg
     1381 cgtctcccac cagcacgatc gtgttgcgca gctgctcgct gatgcgccgc gcgcgcttcg
     1441 agtcgcgctc gatcaccttg acctggttct cgtcctcgag cgactgtgcg agccggaacc
     1501 cgatgttgcc gccgcccgcg atcacgacgc gcttgaccgg gtcctcgagt ttctgcatct
     1561 cccgcatgac gacgcggatg tcctgcgtcg cggccacgaa gaacacctcg tcgccgtcca
     1621 ggatgtcggt gtcaccctcg ggctcgatgc tctggccgct gcgatacatc gccgcgacgc
     1681 gggcctcgct gttgcgcatg tgcgtcttga gttcgcggat cgggcggccg acgagcgcac
     1741 cgccgcgcag cgccttgagc ccgaccaggc gcaccttgcc cgcggcgaag tcgacgatct
     1801 gcagcgcccc ggggtagcgg atcagccgct cgatgtattc ggtcacggcc tgctcggggc
     1861 tcaggtacat gtcgacggcg aacccgtacg gggtgcgttc gttcgctgcg cgcgtcgcgc
     1921 gggcgtcgcc gggcgcggcg tcggtggtca cgaacagccc gtcgtggcgc gtgtagtcgc
     1981 gcgagcggat ccgcgcgatc ttggttcccg tcccgaacag gctccaggcg acgtgacacg
     2041 ccaccatgtt cgtctcgtcg ctgttggtca gcgcgacgat catgtcggcg tcacgcgcgc
     2101 cggcggtctc gagcgtgcgc gggctcgcgg cgttgccctg cacggtgcgg atgtcgatgc
     2161 ggtcctgcag gtcgcgcagc agcgcttcgt cgaggtcgac gacggtgacg tcgttcgctt
     2221 cctcccgggc gaggtggtac gcggccgtgc gcccgacctg cccggccccg aggatgatga
     2281 ttttcaagtt tcttgggcca tctggggctg cgcgccgcca ttctagcgcg gttcaggggc
     2341 gcctccgccg ggtgtcggcg aacgccggcg gtcagacgac ctcgaggtgg gcgtagccca
     2401 tcatgagcca cttggtgccc tggcgctcga agttgacctg tacccgggcg ttggcgccct
     2461 ggccctcgag gttcagtacg acgccgtcgc cgaacttcgc gtgccggacc cgctggccga
     2521 gcctgagccc gccgggcgcc tcgtcctgga accgcttgcc gctcatcgac aggttgccgc
     2581 ccgttggacg cgcagcctgg taaccgcccg ccacgcgcga ctgcggcacg tacgccggcc
     2641 tggagacctg caccttcggg cgcacttcct cgaccagcgg cgcggggatc tcggcgatga
     2701 accgcgacgg tgcgccgtag ctgtcgacgc cgtgcatgcg acgctgctcg gcgtaggtga
     2761 ggtagagctg gcgcatcgcc cgcgtgcagc ccacgtagca gagccggcgc tcctcctcga
     2821 ggccgtgcgt gtcggtgacc gaccgctggt gcggaaacag cccgtcctcg agcccgcaga
     2881 ggaacacgac cgggaactcg agacccttgg ccgaatgcag ggtcatcatc tgcacgcagt
     2941 cttcccaagc ctcggcctgc ccctcgcctg attcgagcac cgcgtgcgag aggaatgcca
     3001 tcaacggggt catgtcgccg tcttcgggct cgaagccgcg agcggcgctc acgagttcct
     3061 cgaggttctc gaggcgcgcc tcgcccttgt ccgccttttc cttctggtag tgagccgcga
     3121 gtccgctcgc ctggatcacg tggtcgacct gctcgtgcag cgggagcccc tgcgtcgcgg
     3181 cgtccagttt ctcgatcagc gcgaggaatg cctgcaggca cgacgcggcc ttgctgccga
     3241 gctcgtcgtt gcactcgaag gcggcccgcc acatcgacgt ggagtgctcg cgcgcatggg
     3301 cgcgcagcac ctcgagcgtg cgggccccga tgccgcgcgt cggcaggttc acgacgcgct
     3361 cgaacgaggc gtcgtcgtcc cggttggcga tcagccgcag gtaggcgagc gcgtccttga
     3421 tctcctggcg ctcgaagaac cggaggcccc cgtagacgcg gtacgggatc cgcgcggcga
     3481 gcaggtactc ttcgaacacg cgagactgcg cattcgagcg gtagaggatc gcgttgtcgc
     3541 ggcgctggcc gccgcgagcg acccattcgc ggatcttgtt gacgacgaac tcggcctcgt
     3601 cgcgctcgtt ccaggcgcgg aacagccgga tcggggcccc acgcccttcg ctggtccaga
     3661 gcttcttgcc gatgcggccg ctgttgttgc cgatgatcgc gttggcggcg tcgaggatgt
     3721 tgccggtcga gcggtagttc tgctcgagcc ggaacagctg cacgtcgcgg aagtccttgc
     3781 ggtactgctg caggttctcg acgcgcgcgc ctcgccagcg gtagatcgac tggtcgtcgt
     3841 cgccgaccac gtagggcacg ctcaggtcgc cgaccagcac gcgcatccac gcgtactgga
     3901 tcgtgttggt gtcctggaac tcgtcgacga gcacgtggcg gaagcgcgcg cgatagtggt
     3961 cgccaagggc cgggacgtcc ttgagcagtt cgtagcttcg cagcagcagc tccgcgaagt
     4021 ccacgacgcc gttgcgtcgg caggcctcct cgtaggcctc gtagagcctg atcatctggc
     4081 gccgggtggg gtcgccgtcg tcacgcaggt gcttcggccg ctcgccttcg tccttgcgag
     4141 cgttgatgaa atacgaaacc tcgcgcggca cccagcgcgt gtcgtcgagg tcgagcccgc
     4201 ggatcacttt cttgacgaga cgctgctggt cctcgccgtc caggatctgg aagccctgcg
     4261 gcagcccggc ctcgcgccag tgcatgcgca gcagccgatg gcagatgccg tggaaggtgc
     4321 cgatccagag cggagcggac ggcagtccga gctgctgctc gatgcgatga cgcatttccg
     4381 cagcggcctt gttggtgaac gtgaccgcga ggatcccgtg gggcgagacg ccctcgacct
     4441 gcaccagcca ttcgacgcgg tgcgtgagca cgcgcgtctt gccgctgccg gccccggcga
     4501 gcacgagcgt gggcttgagc ggcgcagtca cggcctgccg ctgcgcatcg ttcagcggcg
     4561 caagaatgtg ggagacgtcc atcggaaggc tgcggattct accctatcgg tgccggtggc
     4621 acgccttcgg tcgacgggct ccccggcggc ggactcgtgg caggcgcccg cggggttcac
     4681 gacccgcgcc cgcgtggtct acgctcgcgc accagccgag gataccgatg accatgcgaa
     4741 agccgagttt ctcgcgtctt tgtgctcact cctgcctggc cgtgctcgcc ggtctcgcgc
     4801 cgatgggcgc gtcgcttgcc gatgcggcaa gtgccgacgc agcgtggcgg gtgcacgtcg
     4861 aacgtttcgt cgagtcgcac ctcgaggcca acccggtcga ggccgtatac gccggacggc
     4921 acgagttcga tggacgcctg ccggacttga gtgcggccgg catcgcccgc aacgttgcga
     4981 ggctcgaggc tgagcgtgcg aagacggcgg gcttcgaccc ggcgaaactt tcggagccga
     5041 gccggcgcga acgcgagtac ctgctggccg cgctcgccga cgagctgttc tggatccggg
     5101 acgccgagtg gccgttccgc aatccgtcgt ggtacctggg cgtggtcgat ccggaggtct
     5161 acctgagccg gccgtacgcg ccgctcgaga cgcggctgcg cgcctatctc gactatgcgc
     5221 gtgcgatccc ggcagcgacc gagcagatcc gcgcgaacct gcgcacgccg atgccggcgc
     5281 cgtgggtcga atacggcgtc aacgcgttcg gcggcttcgc gcagttcttc gcgaacgacg
     5341 tgggcccggt gttcgccgac gtcgaggacg acgcgctgca atcgcagctc gaggccgcca
     5401 acgccgcggc tgcccgggcg atgcaggcac tcgcggactg gttcgtcgcg cagcggccca
     5461 aggccacgca ggactacgcg ctcggcgcgg gcatgttcgc gaagatgctc gaagcgaccg
     5521 agcaggtcac ggtgccggtc gagcgacttc gcgagatcgg cgaggccgac ctcggtcgcc
     5581 acctcgtcgc gttgcgcaag acctgcgctt cgtacgcgcc cggggcgacg ctgcgggaat
     5641 gcgcggacaa ggccaatgcg cacaagcccg aaggcggcgc ggtcgccggc gcgcgacgcc
     5701 agcttccgga actcaagcag ttcgtggtgc agaagaacct ggcggccatc cccggcacgg
     5761 agcaggcgct ggtcgaggag gcaccgccgt acaacgcgca gaacttcgcg tacatccagg
     5821 tgccgggtcc gtacgagaag acggccgtgc cggccgtcta ctacatcgcg cctcccgacc
     5881 cgcgctggac gccggaggag caggcgcagt acgtctcggg cgaaggcacg ctgctgtcga
     5941 cgtcggtaca cgaagtgtgg cccggtcact tcctgcactt catgcacgtc aatcgcagca
     6001 cttcgaagat cgcgcagctg ttccagagtt acgcctacac ggaaggctgg gctcactaca
     6061 ccgaggagat gatggcggac gagggcctgc gtgccggcga cccggagtgg cacatcggga
     6121 tgcgcctgtc cgcgctcaag cggaacgcgc gctacctgtg cgcgatcggc ctgcacacgg
     6181 ctggcatgac cgtcgcgcag tgcgagaggc tgtttcgcga aagcgcgctg accgaccccg
     6241 gcaacgcccg ccagcaggcg ttgcgtggca cctacgaccc ggcgtacctc aactacaccc
     6301 tcggcaagct gatgatcctg caactgcgcg aggattggcg tgccgcgcac cccaaggcac
     6361 cgctgcgcga cttccacgac cagttcctgg gctacagcgg accggtgccg ctcgtgcgcc
     6421 ggctgatgct cggcaccgtt tcgccgccgc tgtgagcacg cttgacggcc acggtccgca
     6481 cggcggactc cgcctgggcg cggccacccg cgccgacgcg cctgggtgga aagcatgctc
     6541 gcggtgatga cggtaccgtc accgcccggc gagcacccgc ctcacatcgg cgcgaacagc
     6601 tgctggctcg agatgctccg cagcacctcg ccccggttcg tgcgcaaggg atacgtgtcg
     6661 ttcgtgagcc agcgtccgag caggttggcg tagaacgggc tcgtcagcac gccgctcatg
     6721 ccgcccggaa gcgacgtttc ggcctcgatc gagcctggcg ccgtgccggg ggaccccacg
     6781 tagcggcgat tcggtccact gccgaacatg aaggcgtcgg ccgaggcggc gcgcgcgccg
     6841 tgcgaggaag catcgacgac gccgaaaccg ccgtcggtcg cgaggccggc gaggttcgcg
     6901 aacgacggcg ggaagccgag ctgcgcgttc gggatcgaat acgggcctcc caccacgagg
     6961 ccgtcgaaga cgatccggtg cagcttgccc cagcggtagt cgtcctggtt cgtcgatcgc
     7021 gcaaacgcct tctcgaacgc ggggccggcc agcaggtcga gtgcgtcacg caggctgcgc
     7081 agcatcacgt agtcgcgccg ctgctcgggt gtggcgaggc cggtccacgc gaagaagtcg
     7141 accgtggaca gaccgatgcc gttgcgctcg accaggtgtc gcaacgcctt gatcgcctcg
     7201 ccggagccgg gtcgcggtac tccgaggtcg tcgagcgtgc gatcgaccgc gttgttgatc
     7261 gcctgcccgc gccacaccga gtagatcgtc gccgccacgc tgcgtgcgat ctcctgcgac
     7321 gatggcgcgg agagctggcc attcacgtcg accgcgtcgt acccggtcgc aacgccggtc
     7381 ggcgtcgtgt ggttccaggc gccgagccgg tcgacggctt cgacgacccg gggatccgcc
     7441 gccagtgcag cgagctcggc cggcgcgccc gccgacgccg cacgcaggaa agcgcccgtg
     7501 atgtacgggg tcagcacctg cgcgtcgagc aaggtcacgt cggcctggat ggcctgcatg
     7561 tcgctgcgat cgaccgaccc ccggaccaat cgttccttga gcgcctgtgt gatgcgtccg
     7621 gcccgggtgc catggtcgaa ggcatagccc aggtagaaga tgccctggcc gccgattcgc
     7681 aactggttga gcggatcgtt gtcgagcgtc gcgcctgagg tgtcgttgtt ggcattgacg
     7741 acccagccgg ccgaaggatt gacggtctgc ggcagttcct cgaacggcag cgaaaggtat
     7801 cccgtgccgt tgaaggcgtc ggggctcgga tccttgagcc attcgttgcc gccaaaaccg
     7861 tcgcggacga accacggcgg cgccccgcgt acggtgttgg cctgcaggtc ctcgcgcagc
     7921 ggcacctcgc cggtcgtgaa gtagccgatg ttgccctcga tgtcgccgta gatgaaattc
     7981 tgcgagccgg cgtcgaaata gcgcagcgca gtggtgaagt ccgtgaggtc gcgcgcgcgg
     8041 ttcagcagcc ggaacgtgtc cagctcgcgc gttggaccga agccggtgta ttgcacggtc
     8101 accacccggc cgctgccgag cgaggcgacg accggaccct ggttgcgacg cgacagcgtc
     8161 aggacgtcgg cgggcagccg gttctgaggc gtggcgggaa tcgtcacgac cgtgtcggcg
     8221 ccgttgccga gcccgtcgcg caggttcgcc ttgaacgtga gcgggatgcg cgtgacggcg
     8281 ccggtgccgt cctggtggac ggagaccagc ccgctgggcg tgccgggagc cggcgcgagc
     8341 tggtcgacgt acatgtc
//
LOCUS       DASPFR010000113         3569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_887563, whole genome shotgun sequence.
ACCESSION   DASPFR010000113 DASPFR010000000
VERSION     DASPFR010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887563"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /gene="rho"
                     /locus_tag="VD737_02840"
     CDS             <1..207
                     /gene="rho"
                     /locus_tag="VD737_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HXW09524.1"
                     /translation="EKRVYPAFNINRSGTRREELITSSDELQKIWILRKLLHPMDELA
                     AAEFLIDKMRDTKTNGEFFEAMKR"
     gene            237..1097
                     /locus_tag="VD737_02845"
     CDS             237..1097
                     /locus_tag="VD737_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXW09525.1"
                     /translation="MAAMLAAVASFAVMDSFLKLLVAHYPPLQVAFFRGVAALPFVVA
                     TVLVRGRLARLRPVNVPLHLLRGVLSIVMLYSFVSAVRDSSLATTYAIFMCAPLLVAA
                     LSAPLLGERVAAVQWGAIGVGLAGVLLMLKPGPGQIGVAGAVWATVAMVAYTISVVTL
                     RVLARTDSNESMVFWFTAMLAVGAGLLALPGWREFHWAHGWLVAGVGLTGATGQYLIT
                     LAFRHAPAATVTPFEYTALVWGVLLDVAIWSVWPSVVTLLGGAVVIGAGLYLIERERR
                     TSAAAAVPGA"
     gene            complement(1109..1546)
                     /locus_tag="VD737_02850"
     CDS             complement(1109..1546)
                     /locus_tag="VD737_02850"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HXW09526.1"
                     /translation="MMLRNLLLGLPATLACVALQVTFAYWSVRYYVRRSVAAPRASGL
                     WPLMVTMLIMMSGTILQIVLWAALFVGLDEFDEFYDAVYFSTVNFSSLGYGDVVMTRP
                     WKLLGPLEALCGVLMLGMTAAALMAILQQMIRSHRAALGAPTE"
     gene            complement(1543..2592)
                     /locus_tag="VD737_02855"
     CDS             complement(1543..2592)
                     /locus_tag="VD737_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09527.1"
                     /translation="MTRPDKAVLRLTIGLGIAALVAYGGGLQMPYALCVMAVLVLCKP
                     GPPMGLGKGAVAAAALAGLLALGMLMVPLLESYAVAGIILTACVLFAVFHRGQRTKSP
                     LTMILVMAFALIPVLGVADQALAATITTTLALGLLIGAVVSLLSHGFFPDPPPAAGAP
                     ASRASRADQEQARWIALRATLIVMPVFVLALVNPSLYVAAIMKTVALGQQAGETNARS
                     AGVELVGSTLMGAALGLVAWLGLSLLPNLWMLVLWLMALALWTGSAMYGTRRTRHGPS
                     FWSNALITMLILLGPAIEDSAAGKSVLAASAVRTTLYLGVSLYAWGTVWALERWRATW
                     ARRERRRLHTLEAHA"
     gene            complement(2603..>3569)
                     /locus_tag="VD737_02860"
     CDS             complement(2603..>3569)
                     /locus_tag="VD737_02860"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HXW09528.1"
                     /translation="VQAFVVPVAAEVSGKVLKVHIRNDDRVRPGQPLFEVDPDQYEIA
                     AQRARSDYESVRRSVNASAAGVDAARASLRAAEAGRDMAEKDAARQERLYAEDPGAIS
                     VRRLEIAQSTREEARSKARRAEADLVKAQEAAGDAGDNNAQLRSARAAVEKAELDLAR
                     TTVVAPAAGTVTDLRVDVGNFAQAGAPVMTLIAVHDVWITADLTENNLGHVDVGDEVA
                     VTFDVMPGRVFEGRIRSIGGGVGSGPSSPPGTLPTVQNNRDWLRQAQRIPVIVELDPA
                     ERPDMRRVRVGGQAAVLVYTDDDNGLMNALGAAYIRVMSWLSYLY"
BASE COUNT          535 a         1277 c         1230 g          527 t
ORIGIN      
        1 gagaagcgcg tctacccggc gttcaacatc aaccgttccg gaacgcgccg cgaggaactg
       61 atcacctcgt cggacgaact gcagaagatc tggatcctgc gcaagctcct gcacccgatg
      121 gacgagctgg ccgcggccga gttcctgatc gacaagatgc gcgacaccaa gaccaacggt
      181 gagttcttcg aggcgatgaa gcgctgaagg ggacgcacga ccaccgcctg cgcggcatcg
      241 cggcgatgct ggcggccgtc gccagcttcg cggtgatgga cagcttcctc aagctgctgg
      301 tcgcgcacta cccgcccctg caggttgcct ttttccgcgg cgtcgccgcg ctgccgttcg
      361 tcgtcgccac ggtgctggtg cgcggtcgac tcgcacgcct tcgtcccgtc aacgtgcccc
      421 tgcacctgct gcgcggcgtg ctttccatcg tgatgctcta cagcttcgtg tcggccgtgc
      481 gcgactcctc gctcgccacg acgtatgcca tcttcatgtg tgcgccgctg ctcgtggcgg
      541 cgctctcggc gccgttgctc ggcgaacgtg tggccgccgt gcaatggggc gcgatcggcg
      601 tcggcctggc cggcgtgctg ctgatgctga agcccggtcc gggccagatc ggcgtcgcgg
      661 gcgcggtgtg ggcgaccgtc gcgatggtcg cgtacacgat ctcggtcgtg acactgcgcg
      721 tgcttgcacg caccgacagc aacgaaagca tggtgttctg gttcacggcg atgctcgccg
      781 tcggcgcggg gctgctcgcg ctccccggct ggcgcgagtt ccactgggcg cacggctggc
      841 tggtcgccgg ggtcgggctc accggcgcga ccgggcagta cctgatcacg ctggcgttcc
      901 ggcacgcgcc tgcggcgacg gtgacgccgt tcgaatacac cgcgctggtc tggggcgtgc
      961 tgctcgacgt cgcgatctgg agcgtctggc cgagcgtcgt cacgctgctg ggcggtgccg
     1021 tcgtgatcgg cgccggcctg tacctgatcg agcgggaacg gcgcacctcc gcggcggccg
     1081 cggttccggg cgcctgagcc ggggcgcctc actccgtcgg ggcaccgagc gccgcacggt
     1141 gcgagcggat catctgctgc aggatcgcca tcagtgccgc cgccgtcatg cccagcatca
     1201 gcacgccgca gagggcctcg agcggcccga gcagcttcca gggccgcgtc atgacgacgt
     1261 ccccgtaacc cagcgacgag aagttgaccg tcgagaaata gacggcgtcg tagaactcgt
     1321 cgaattcgtc gaggccgacg aacaacgcgg cccacaacac gatctgcagg atcgttccgg
     1381 acatcatgat cagcatcgtg accatcaggg gccacaggcc cgacgcgcgc ggtgcggcca
     1441 ccgagcgccg cacgtagtag cggacgctcc agtaggcgaa cgtgacctgc agggcgacgc
     1501 aggccagcgt cgccggcagc ccgagcagca ggttacgcag catcatgcgt gtgcctccag
     1561 tgtgtgcagt cgccgccgtt cccggcgagc ccacgtggcg cgccagcgct cgagcgccca
     1621 gaccgtgccc cacgcataca gcgagacgcc gaggtagagc gtcgtgcgca cggcggacgc
     1681 ggccagcacg ctcttgccgg cagcgctgtc ctcgatcgcg ggcccgagca ggatcagcat
     1741 cgtgatcagg gcgttgctcc agaacgacgg cccgtgtcgc gtgcggcggg tgccgtacat
     1801 cgccgagccc gtccacagcg cgagtgccat cagccagagc accagcatcc agaggttggg
     1861 caacaacgag aggccgagcc aggcgacgag gccgagcgcg gcgcccatca gcgtggaacc
     1921 gaccagctcg accccggccg agcgcgcatt cgtctcgccg gcctgctgtc cgagcgcgac
     1981 cgtcttcatg atcgcggcca cgtacagcga cgggttcacc agggccagca cgaacacggg
     2041 catgacgatc agcgtcgccc gcagcgcgat ccagcgcgcc tgctcctggt cggcgcggct
     2101 cgcgcgcgac gccggcgcgc ccgcggccgg cggcgggtcg ggaaagaacc cgtgcgagag
     2161 caggctgacg accgccccga tcagcaggcc cagggcgagc gtggtcgtga tcgtggcggc
     2221 gagcgcctgg tccgccacgc cgagcacggg aatcagcgca aaggccatca cgaggatcat
     2281 ggtgagcggg cttttcgtac gctgaccccg gtggaacacc gcgaacagca cgcaggcggt
     2341 caggatgatc ccggccacgg cgtagctttc gagcagcggc accatcagca ttccgagcgc
     2401 aagcaggccg gccaacgccg cggcggccac ggcccccttg ccgagcccca tgggcgggcc
     2461 gggcttgcac agcaccagca cggccatcac gcacagggcg tacggcatct gcagtccgcc
     2521 accgtaggcg accagggcgg cgatgccgag cccgatcgtc aatcgcagca ccgccttgtc
     2581 gggacgtgtc atggcgtgca cgtcagtaca ggtacgacag ccagctcatg acccggatgt
     2641 aggcggcgcc gagcgcgttc atcagcccgt tgtcatcgtc ggtgtagacg agcaccgcgg
     2701 cctgaccccc gacccgcacg cggcgcatgt ccggccgctc ggccggatcg agttcgacga
     2761 tcacggggat gcgctgcgcc tgccgaagcc agtcccggtt gttctgcacg gtgggcagcg
     2821 tcccgggcgg cgacgacggg ccgctgccca cgccaccgcc gatgctgcgg atgcggccct
     2881 cgaagacccg cccgggcatc acgtcgaagg tgacggccac ctcgtcaccc acgtcgacgt
     2941 ggccgaggtt gttctcggtc aggtccgccg tgatccagac gtcgtgcacg gcgatcagcg
     3001 tcatgaccgg tgcgcccgcc tgcgcgaagt taccgacgtc gacgcgcagg tcggtgacgg
     3061 tgccggcggc cggcgccacc accgtggtgc gggcgaggtc gagctcggcc ttctcgaccg
     3121 cggcgcgcgc gctgcgcagc tgcgcgttgt tgtcgcccgc gtcgcccgcg gcctcctgcg
     3181 ccttgaccag gtccgcctcg gcgcgtcgtg ccttgctgcg cgcctcctcg cgcgtcgact
     3241 gtgcgatctc gagccgtcgc acggaaatcg cccccggatc ctcggcgtac agccgttcct
     3301 gccgcgcggc gtccttctcc gccatgtcgc gacccgcttc tgccgcgcgc agcgaagcgc
     3361 gggccgcgtc cacgccggcc gcggacgcgt tgaccgaacg acgcaccgac tcgtagtcgg
     3421 agcgtgcccg ttgcgcggca atctcgtact ggtcgggatc gacctcgaac agcggttggc
     3481 cgggccgcac ccggtcgtcg ttgcggatgt gcaccttcag caccttgccc gagacctcgg
     3541 cggccaccgg cacgacgaat gcctggaca
//
LOCUS       DASPFR010000114         3128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_277908, whole genome shotgun sequence.
ACCESSION   DASPFR010000114 DASPFR010000000
VERSION     DASPFR010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277908"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VD737_02865"
     CDS             <1..201
                     /locus_tag="VD737_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09529.1"
                     /translation="KVGSSLSDKLMLAESYYEVPDVAAGCSMMSDFMAQVRALAGKKF
                     TDALAAELADGAQQVMTNHGCQ"
     gene            complement(221..871)
                     /locus_tag="VD737_02870"
     CDS             complement(221..871)
                     /locus_tag="VD737_02870"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXW09530.1"
                     /translation="MNKPPLPFTLLEAPEVLANCYGWSEEVYRDLMALQDGAVSQHEL
                     DRKYLHRKAILMLDFTGFTIQAMHGQQVQALLRILDAQRVCVPVLHEHGATLVRAFAD
                     DLVGLFDDPGHALDAAFEIHRRVGIFNHSPHAAEKPALACVGIGYGEVYAIGPNLAMG
                     DEMNRASKLGEDIARGNETLVTSNVYFALRGRGDVQFEQLASDDQLFPYYRASPPG"
     gene            complement(906..1715)
                     /locus_tag="VD737_02875"
     CDS             complement(906..1715)
                     /locus_tag="VD737_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXW09531.1"
                     /translation="MIGSPTAPVRLLHVTDPHLFGDESRTIYGVQTATSLRAVLAEAL
                     APGAPRPHAILVTGDIADDHTPEAYANFRRALEPFGLPVFCLPGNHDEPALMPALVGH
                     SGFQYGGAAEFGAWGVVFLDTHVPGRPEGHVARDELVRLHDELERMHDRPVLVCLHHP
                     PLPVGSAWLDGVGLTNADALLEVLDRHPTVRLVLGGHVHQAFAQRRHGALVLATPSTC
                     AQFTPRTERCVMDLKPPGYRWLELLPDGGVRTEVRWLQGWAVTGRPPDDRF"
     gene            complement(1712..3052)
                     /locus_tag="VD737_02880"
     CDS             complement(1712..3052)
                     /locus_tag="VD737_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXW09532.1"
                     /translation="MNAAEAPVVVENLSHYFGQGALRRQILFDVSTTIPAGEIVIVTG
                     PSGSGKTTLLTLVGALRAPQQGGLRVLGQRLDGARRGALEDVRRRIGFVFQQHNLLGA
                     LTASQNVQLGLRVTGRAARAELKRRADAMLEAVGLGQHTHKRADQLSGGQRQRVAIAR
                     ALVGEPAMVLADEPTASLDKASGREVVDRMQGLARERGTTILLVTHDNRILDVADRIL
                     HLEDGRLATFTDAVISNTQQMMHLLAQNTERQDLVRVIAGMAEPEFRRALQDTTQQAQ
                     RFLEATSLASDEAYQSMLEQSLRMFTRRVGELLDAERASMFLVDRTRQQLVLRVAQDA
                     PTGAHVRIPLSSGIAGAAATSGVPVRVDDAYADPRFNRAVDAETGFRTRSVLCLPLRD
                     RAGEVFAVTQLLNRRDGLPFDEADERRYADFAASLSVLLESLARIDAGDRGTIG"
BASE COUNT          476 a         1080 c         1094 g          478 t
ORIGIN      
        1 aaggtcggct cgagcctgtc tgacaagctg atgctcgcgg agtcgtatta cgaggtgccg
       61 gacgtcgcgg ccggttgttc gatgatgtcg gacttcatgg cgcaagtccg ggcgcttgcc
      121 ggcaagaagt tcacggatgc gctggccgcg gagctcgccg atggcgcaca gcaggtgatg
      181 acgaatcacg gctgccagtg aaggctcgca ccgcgggccg ctatcccgga ggagacgccc
      241 ggtagtacgg aaacagctgg tcgtcgctcg ccagctgctc gaactgcacg tcgccgcgcc
      301 cacgtagcgc gaagtagaca tttgaagtca cgagcgtctc gttgccgcgt gcgatgtcct
      361 caccgagttt cgacgcgcgg ttcatctcgt cgcccatggc caggttgggt ccgatggcgt
      421 acacctcgcc atagccgatg ccgacacagg cgagcgccgg cttctctgcc gcgtgcggcg
      481 agtgattgaa tatcccgacg cgccggtgga tctcgaacgc ggcgtcgagc gcgtgtccag
      541 ggtcgtcgaa caggccgacc aggtcgtcgg caaaggctcg caccagggtc gccccgtgct
      601 cgtgcagcac cggcacgcag acgcgctgcg catccagtat gcgcagtagc gcctgcacct
      661 gctggccgtg catggcctgt atggtgaacc cggtgaaatc cagcatcagg attgccttgc
      721 gatgcaggta tttgcggtcg agttcgtgct ggctcactgc gccgtcctgg agcgccatca
      781 ggtcgcgata gacctcctcg ctccagccgt agcagttcgc cagcacctcc ggagcctcga
      841 gcagggtgaa cggcagtggc ggcttgttca tcgcggcata ctcctgcagg ccttggcgtc
      901 gtcgttcaga agcgatcgtc cggcgggcgc ccggtcaccg cccacccttg cagccaccgc
      961 acctcggtgc gcacgccgcc gtcgggcagc agttcgagcc agcggtaacc cggcggcttc
     1021 aggtccatca cgcaccgctc ggttcgcggc gtgaactgtg cgcaggtcga cggggtcgcg
     1081 agcacgagcg cgccgtggcg gcgttgagcg aatgcctgat gcacgtggcc accgagcacc
     1141 aggcgcaccg tcgggtgccg gtcgagcacc tcgagcagtg cgtcggcgtt ggtgaggccc
     1201 acgccgtcga gccacgcgct gccgacgggc agcggcggat gatgcaggca caccaggacg
     1261 gggcgatcgt gcatgcgctc gagttcgtcg tgcagtcgaa cgagctcgtc gcgggcgacg
     1321 tgcccttccg ggcgcccggg cacgtgcgtg tccaggaaca cgactcccca cgcgccgaac
     1381 tcggccgcgc cgccatactg gaagccggaa tggccgacca gcgcgggcat cagcgccggt
     1441 tcgtcatggt tgccaggcag gcagaagacc ggcaacccga acggctccag cgcgcgacgg
     1501 aagttggcgt acgcctccgg cgtgtggtcg tccgcgatgt cgcccgtgac caggatcgcg
     1561 tgcggccgcg gcgcgcccgg cgccagcgct tcggcgagca ccgcacgcag cgacgtggcc
     1621 gtctgcacac cgtagatcgt gcgcgactcg tcgccgaaga ggtgcgggtc ggtcacgtgc
     1681 agcagtcgca cgggcgcggt gggcgaaccg atcaaccgat cgtcccccgg tcacccgcgt
     1741 cgatgcgcgc gagcgactcg agcagcaccg acagcgaggc cgcgaagtcg gcgtaacgac
     1801 gttcgtccgc ctcgtcgaac gggaggccgt cgcggcggtt gagcagctgc gtgacggcga
     1861 agacttcgcc tgcacggtca cgcagcggca ggcacagcac gctgcgtgtg cggaaaccag
     1921 tctccgcgtc caccgcccgg ttgaagcgcg ggtcggcgta ggcatcgtcc acgcgcacgg
     1981 gtacgccgga cgtcgcggcc gcacccgcga tgccgctgga caacgggatc cggacgtgcg
     2041 ccccggttgg cgcgtcctgc gcgacgcgca atacgagctg ctgccgcgtg cgatcgacga
     2101 ggaacatcga cgcgcgttcg gcgtcgagca actccccgac ccgtcgcgtg aacatgcgca
     2161 gcgactgctc gagcatgctc tggtacgcct cgtcgctggc gagcgaggtg gcctcgagga
     2221 agcgctgcgc ctgctgcgtg gtgtcctgca gcgcgcgccg gaactccggc tccgccatgc
     2281 cggcgatcac tcgcacgagg tcctggcgct ccgtgttctg cgccagcagg tgcatcatct
     2341 gctgcgtgtt cgagatcact gcgtcggtga acgtcgcgag ccggccgtcc tcgaggtgca
     2401 ggatgcggtc ggccacgtcg aggatgcgat tgtcgtgcgt gaccagcagg atcgtggtgc
     2461 cgcgctcgcg ggcgagcccc tgcatgcgat cgacgacctc gcggccggaa gccttgtcga
     2521 gcgacgccgt cggttcgtcg gcgagcacca tcgccggctc gccgaccagc gctcgcgcga
     2581 tcgcgacgcg ttgtcgttgg ccgccggaca gctggtcggc acgcttgtgc gtgtgttgcc
     2641 cgagaccgac cgcctccagc atcgcgtcgg cgcggcgctt gagctcggcg cgggccgcgc
     2701 gccccgtcac gcgcagcccg agctgcacgt tctgcgacgc cgtcagcgcg ccgagcaggt
     2761 tgtgctgttg gaagacgaag ccgatgcgcc ggcgcacgtc ctcgagcgcg ccccggcgcg
     2821 cgccgtcgag ccgctgaccc agcacgcgca gcccgccctg ctgcggcgcg cgcaacgcgc
     2881 cgaccagcgt cagcagcgtg gtcttgcccg agcctgacgg ccccgtgacg atcacgatct
     2941 cgccggcggg tatcgtcgtc gaaacgtcga acagtatctg gcggcgcagc gccccctggc
     3001 cgaaatagtg gctcaggttc tcgacgacga ccggtgcctc ggcagcgttc atgcctagaa
     3061 cacgtcggcc gggtcgagcc ggcgcacctt gcgcagcgcc agcaggcccg acaacgcgca
     3121 catcacga
//
LOCUS       DASPFR010000115         4920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1254563, whole genome shotgun sequence.
ACCESSION   DASPFR010000115 DASPFR010000000
VERSION     DASPFR010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254563"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VD737_02885"
     CDS             complement(<1..445)
                     /locus_tag="VD737_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016711990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXW09533.1"
                     /translation="MSRLKVTETFVSIQGEADAVGWPTLFIRLTGCPLRCVYCDTQYA
                     FHGGEWQTLDQLLEVAHGSGVRHVCVTGGEPLAQKACLELLTALCDAGFEVSLETSGA
                     LDVAPVDPRVSRVVDLKTPGSGEERRNRIENLEVLAERDQLKFVLC"
     gene            complement(442..1206)
                     /gene="ybgF"
                     /locus_tag="VD737_02890"
     CDS             complement(442..1206)
                     /gene="ybgF"
                     /locus_tag="VD737_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HXW09534.1"
                     /translation="MIRNRSLAVAIVGVLSFGVPALAFGTASNKELEQRANQTDSRID
                     ELEARLNQSLLDLQRQIAGSQQELRTLRGQIEEARHELETLRQQQRDLYADHDRRLLA
                     IENGAALGGVPPATGGLANPPDVVFADEASVYGEAFAAMKAGRYEEAARGFETYLAKY
                     PRGPRADNATYWLGEAQFMQQQYEAALKSFQAVASFPESRRLAEALLKVGDSQAALKA
                     FRNARASYQAVISTYPESAPAAQARMRIEKLNAEGR"
     gene            complement(1211..1747)
                     /gene="pal"
                     /locus_tag="VD737_02895"
     CDS             complement(1211..1747)
                     /gene="pal"
                     /locus_tag="VD737_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HXW09535.1"
                     /translation="MKWTHALAAAVLALGLAACQKPKQVQPEPATPTTPAESGDASTS
                     GIGTDAGATAGAMSEAQRQALAALQARNVVYFDYDSSELKPEYDAVIATHAGYLAQYK
                     TARVRLEGHTDERGSREYNIGLGERRAQAVRRALMLQGVAEGQITTVSYGEERPVAEG
                     SDEGAYSQNRRVELVQVQ"
     gene            complement(1866..3203)
                     /gene="tolB"
                     /locus_tag="VD737_02900"
     CDS             complement(1866..3203)
                     /gene="tolB"
                     /locus_tag="VD737_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HXW09536.1"
                     /translation="MTKNLHGWARRSRAAAGLVAAAALWLCAATAEAQLRLDITQGVR
                     DAVPIAVVPFGGQAEGAPTDVAAVVANDLLLSGRFKPLERRDMVMRPTTGAAIRFEDW
                     RLLRADFIVVGRVEPDATGLAVTFELYNVQTGQPLLSQRLATTERGLRATAHRISDLV
                     FERLTGIPGAFSTRIAYVTVEGRPPKQRYRLVVADADGYGPRTVTESSEPLMSPAWSP
                     DGQSLAYVSFEGKASAVYVQRLATGERRRVSARLGINGAPAWSPDGRKLALTLSRDAN
                     LDIYVLDLATQGLTRLTTDGSIDTEPEWSRDGATVYFTSDRSGSAQVYRIATAAGQQP
                     QRLTFTNSYNARPRLSPDGQSLALVTLDRGGYRIATFDLKTRNVQVLTDGRQDEAPSF
                     APNGAVIIYATRSGNRGTLAMVSSDGRFQQRLSSDTGDVREPVWSPYPATAQR"
     gene            complement(3211..4128)
                     /gene="tolA"
                     /locus_tag="VD737_02905"
     CDS             complement(3211..4128)
                     /gene="tolA"
                     /locus_tag="VD737_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HXW09537.1"
                     /translation="MTRRLRDRARPWLGSAALHVAFVVVLIVAAYNWRSDPPPQQLAI
                     EGDVVRYEDLPPSVRSGKPLREPQPTPPPEAPPEPAPTPPEPAPQPPPEPEAAKLTAE
                     REAAEALKVEQARLAAEREAEQQRAAELEQARAAEARREQEAEALAARQRAAQEAEAR
                     RKAEEDKQREALAAKARQAEQEKAAREAKARAEREAALQRELASEEEREAFARSGVVD
                     EYRTLLTQTIERNWNRPDSARAGLKCTLHVTQAPTGMVMDVRIGECNGDQAVRESITT
                     AVYRSSPLPAPRDPRAFQRQLVMVFKPTE"
     gene            complement(4133..4579)
                     /gene="tolR"
                     /locus_tag="VD737_02910"
     CDS             complement(4133..4579)
                     /gene="tolR"
                     /locus_tag="VD737_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HXW09538.1"
                     /translation="MAGLRRGRKLMGEINVVPYIDVMLVLLIIFMVTAPMLSQGIKVD
                     LPQAAAEPIQPDDLEPLVLSVDPAGEMYLNLGDPQRALAPERVFELASASLRREPERP
                     VLVKADRAVAYGRVVEGMVLLQRAGARKVGFVTDPVATSREPARQR"
     gene            complement(4590..>4920)
                     /locus_tag="VD737_02915"
     CDS             complement(4590..>4920)
                     /locus_tag="VD737_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXW09539.1"
                     /translation="KEIDRLEQNLATLATIGSTSPYVGLFGTVWGIMSAFQGLGSVQQ
                     ATLAMVAPGIAEALIATAMGLFAAIPAVIAYNRYADQVGRLELRFDTFMEELSTILQR
                     HAGVQAG"
BASE COUNT          643 a         1780 c         1704 g          793 t
ORIGIN      
        1 agcacagcac gaacttgagc tggtcgcgtt cggcgaggac ctcgaggttc tcgatccggt
       61 tgcgtcgctc ctcgcccgag cccggcgtct tcaggtcgac gacgcggctc acgcgcggat
      121 ccaccggcgc gacgtcgagg gccccgctgg tctccagcga gacctcgaaa ccggcatcac
      181 acagcgcggt cagcagctcc aggcaggctt tctgcgcgag cggctcgccg ccggtgacgc
      241 agacgtgccg cacgccgctg ccgtgcgcga cctcgagcag ctggtcgagc gtctgccact
      301 cgccaccgtg gaacgcgtac tgcgtgtcac agtacacgca gcgcagcggg cacccggtca
      361 gccggatgaa cagcgtcggc cagccgacgg catccgcctc accctggatc gagacgaagg
      421 tctcggtgac cttgagccgg ctcaccgacc ctcggcgttg agcttttcga tccgcatgcg
      481 cgcctgcgcg gccggggcgg attccgggta ggtcgaaatc accgcctggt aggaggcccg
      541 tgcgttgcgg aacgccttca gcgccgcctg ggagtcaccg accttgagca gcgcctcggc
      601 cagccgtcgc gattccggga agctcgcgac ggcctggaac gacttgagcg cggcctcgta
      661 ctgctgctgc atgaattgcg cctcgccgag ccagtacgtc gcgttgtcgg cacggggacc
      721 gcgcggatac ttggcgagat acgtctcgaa gccgcgcgcg gcctcctcgt accggccggc
      781 cttcatggcg gcgaacgcct cgccatagac cgaggcttcg tcggcgaaca cgacgtcagg
      841 cggattcgcg agcccgccgg tcgcgggagg cacgccgccc agcgccgcgc cgttctcgat
      901 cgcgagcagc cgccggtcgt ggtccgcgta caggtcgcgc tgctgctggc gcagcgtctc
      961 gagctcgtgc cgggcttcct cgatctggcc ccgcagcgtg cgcaattcct gctgcgagcc
     1021 cgcgatctgc cgctgcaggt cgagcagcga ctgattcagt cgcgcctcga gctcgtcgat
     1081 ccgcgagtca gtctggttcg cgcgctgctc gagctccttg ttgctcgcgg tgccgaacgc
     1141 gagcgccggc acgccgaacg acagcacgcc gacgatcgcg acggcgaggg atcggttgcg
     1201 gatcatggcg tcactgcacc tgcacgagtt cgacgcgacg gttctgggag tacgcgcctt
     1261 cgtccgagcc ttcggcgacg ggccgctcct cgccgtagct cacggtcgtg atctggccct
     1321 cggcgacgcc ctgcagcatc agcgcccggc gcaccgcctg cgcgcgccgc tcgccgaggc
     1381 cgatgttgta ctcgcgcgag ccgcgctcgt cggtgtggcc ttcgagccgc acgcgcgcgg
     1441 tcttgtactg ggcgaggtag cccgcgtgcg tcgcgatgac tgcgtcgtac tccggcttga
     1501 gttcgctgct gtcgtaatcg aagtagacca cgttgcgggc ctgcagcgcc gcgagcgcct
     1561 gccgctgcgc ctcgctcatg gctccggccg tcgcgcccgc gtccgtgccg atgccgctcg
     1621 tcgacgcgtc gcccgactcg gcaggggtcg tgggcgtcgc gggttcgggc tgcacctgct
     1681 tcggcttctg gcaggcggcc aggccgaggg cgagcacggc cgcggcgagg gcatgggtcc
     1741 acttcatgtt gatcggctcc ttggtcgggc aatggccgcg tgacgcggcg aatcggggac
     1801 gcatcatacc gcggcagtct ccgcacggac ccacctcggc tggcgtggga tccggcctcg
     1861 ccgcttcatc gctgcgcggt ggccggatac ggcgaccaca ccggttcccg cacgtctccg
     1921 gtgtcggagc tgagccgctg ctggaagcgc ccgtcggacg agaccatcgc gagggtgccg
     1981 cggttgccgc tgcgcgtcgc gtagatgatc actgcaccat tcggcgcgaa gctcggcgct
     2041 tcgtcctggc ggccgtcggt caggacctgc acgttgcgcg tcttcaggtc gaacgtcgcg
     2101 atccggtagc cgccgcgatc gagcgtcacg agcgcgagcg actggccgtc cggcgacagc
     2161 cgtggccgcg cgttgtagct gttcgtgaag gtgaggcgct gcggctgctg gcccgccgcc
     2221 gtcgcgatcc ggtagacctg tgcactgccg gaacggtcgg acgtgaaata gacggtcgcg
     2281 ccgtcgcgcg accattccgg ttcggtgtcg atcgagccgt ccgtcgtgag ccgcgtgagg
     2341 ccctgtgtcg cgaggtcgag cacgtagatg tcgagattgg cgtcgcggga gagggtcaac
     2401 gcgagcttgc ggccgtccgg cgaccaggcc ggcgcgccgt tgatgccgag ccgcgccgac
     2461 acgcggcgcc gttcgcccgt cgcgagccgc tgcacgtaca cggcggaagc cttgccctcg
     2521 aacgagacgt acgcgaggct ctggccgtcg ggcgaccacg ccggggacat cagcggctcc
     2581 gacgattcgg tgacggtgcg cgggccgtac ccgtcggcgt ccgcgacgac gagccggtag
     2641 cgctgcttcg gcgggcgtcc ttccacggtc acgtacgcga tgcgggtcga gaacgccccg
     2701 gggatgcccg tcaatcgttc gaagaccagg tcggagatgc ggtgcgccgt cgcgcgcagc
     2761 ccgcgctcgg tcgtcgcgag ccgctggctg agcaatggct gtccggtctg cacgttgtag
     2821 agctcgaacg tcacggcgag gccggtggcg tccggttcga cgcgaccgac gacgatgaag
     2881 tccgcacgca gcagccgcca gtcctcgaag cggatcgcag caccggtcgt gggccgcatc
     2941 accatgtctc gacgctcgag cggtttgaac cgaccgctca gcagcaggtc gttcgcgacg
     3001 acggccgcca cgtcggtcgg cgcgccttcg gcctggccgc cgaacggaac gaccgcgatc
     3061 ggcaccgcgt cgcgcacccc ctgggtgatg tccagtcgca gctgcgcctc ggccgtcgct
     3121 gcgcacaacc acaacgcggc cgcggccacc aaccccgcgg cggcccgtga ccgcctcgcc
     3181 cacccgtgca gattcttcgt caaggtgcgt ctactccgtc ggcttgaaca ccatcaccag
     3241 ctggcgctgg aacgcgcgag gatcgcgcgg cgccggcaac ggtgacgaac gatacacggc
     3301 agtcgtgatc gattcgcgca cggcctggtc gccattgcac tcgccgatcc tgacgtccat
     3361 caccatgccg gtcggcgcct gcgtgacgtg cagcgtgcac ttgagcccgg cccgcgccga
     3421 atcgggcctg ttccagttgc gttcgatcgt ctgcgtgagc agcgtccggt attcgtcgac
     3481 gacgcccgag cgtgcgaacg cctcgcgctc ctcctcgctc gccagctccc tctgcagcgc
     3541 ggcctcccgc tcggcccggg ccttcgcctc ccgcgccgct ttctcctgtt cggcctggcg
     3601 cgccttggcc gccagcgcct cgcgctgctt gtcctcctcg gccttgcgcc gcgcctcggc
     3661 ctcctgcgcg gcacgctgcc tggccgcgag cgcctcggct tcctgctcgc gacgcgcctc
     3721 cgccgcgcga gcctgctcga gttccgcggc tcgctgctgc tcggcctcgc gttcggcggc
     3781 cagccgcgcc tgctcgacct tcaacgcctc ggcggcctcg cgctcggcgg tgagcttcgc
     3841 ggcctccggc tcgggtggcg gctgtggtgc gggctcgggt ggcgtcggcg ccggctccgg
     3901 tggagcctcg ggcggcggcg tcggttgcgg ctcgcgcaac ggcttgcccg agcgcacgga
     3961 cggcggcagg tcctcgtaac gcaccacgtc gccttcgatc gcgagctgct gcggcggcgg
     4021 gtcgctgcgc cagttgtagg ccgcgacgat cagcaccacg acgaacgcga cgtgcagcgc
     4081 cgccgacccc agccagggcc gtgcgcgatc gcgcagacga cgggtcatcg ggtcagcgct
     4141 gcctcgccgg ctcgcggctc gtagcgaccg ggtcggtcac gaaaccgact ttcctggctc
     4201 cggctcgttg cagcagcacc atgccctcca cgacgcggcc gtacgccacg gccctgtccg
     4261 ccttgaccag tacggggcgc tcgggttccc ggcgcaggct cgcggaggcc agctcgaaca
     4321 ccctttccgg ggcgagcgcg cgctgcggat cgcccaggtt caggtacatc tcgcccgcgg
     4381 gatcgacgct gagcaccagc ggctcgaggt cgtcgggctg gatcggctcg gccgccgcct
     4441 ggggcaggtc gaccttgatg ccctggctca gcatcggtgc cgtcaccatg aagatgatca
     4501 gcagcaccag catcacgtcg atgtacggca cgacgttgat ctcgcccatg agtttgcgac
     4561 cgcgccgcag tcctgccatg tccgtaccct cagccggcct gcacgccggc gtggcgctgc
     4621 aggatcgtgg acagctcttc catgaacgtg tcgaagcgca gctccagccg cccgacctgg
     4681 tcggcatagc ggttgtaggc gatcaccgcc gggatcgcgg caaacagacc catcgcggtc
     4741 gcgatcagcg cttcggcgat gccgggcgcg accatggcga gcgtcgcctg ctgcacgctg
     4801 cccagcccct ggaaggcgct catgatgccc cacaccgtgc cgaagagccc gacgtagggg
     4861 ctggtcgagc cgatggtggc gagcgtcgcc aggttctgtt ccaggcggtc gatctccttg
//
LOCUS       DASPFR010000116         4432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_279310, whole genome shotgun sequence.
ACCESSION   DASPFR010000116 DASPFR010000000
VERSION     DASPFR010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279310"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /locus_tag="VD737_02920"
     CDS             <1..1407
                     /locus_tag="VD737_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HXW09540.1"
                     /translation="FRTGSQCGADDVTMCLAGLPDFRQEQAGVTEFLNGTIRYLRDNV
                     PGLDGLRMDTMKHVGDAYWRQFFATGGVAAPATLWTVGEIFSSDVAQVAKYVDALGSP
                     SAFDFPLKFAVTDSLGKGQSTRRLADLFARDATYDDPTRLATFLDNHDVWRFTSEAEA
                     AGATAAEADRRLDMALTLLYAARGIPVVYYGTEIAMRGRGDSYSLPLGQSSREDMQFQ
                     RLAESTFDERLAALAQARTSRAALRRGSQRTLAAPGATCTAPSSSLSPSADFGDALYV
                     RGSFDDWANPPATANRFANLGSRQYLAVAELPAGQIEFKVAAADWTPEFSNTTQATVP
                     GVPITLSTNSGAGTNSRLQAGTSGCYEFALNATSTSAPVLTVSARSTAAEPDVFAFVR
                     TLAGESSVVYVVNAERAGVDLAALAGGGIDVGGIFADGAALTEITGNATAAQALRVAG
                     GRLVGTLPALTAVLLAAN"
     gene            complement(1448..3094)
                     /locus_tag="VD737_02925"
     CDS             complement(1448..3094)
                     /locus_tag="VD737_02925"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HXW09541.1"
                     /translation="MHKRPLPPLPEWFPRGAALLRDPTLNKGTGFTEQERDVLGLRGL
                     LPAHVCTQDEQVARALENFRRLQTPLSKYIMLSSLLDRNEALFYRVVTDHPDEMMPII
                     YTPTVGLACQQFGHIYRRPRGLFVSATDRGRIAQVMRNWPHREAAMIVVTDGERILGL
                     GDLGANGMGIPIGKLSLYTACAGLHPWKCLPVMLDVGTNDAALLEDPLYLGLRQRRLT
                     GAAYDELVEEFMVASQEVFPGIVVQFEDFANHNAFRLLRRYRDRICCFNDDIQGTAAV
                     TVAGILSSLRVTGQRMRDQRFLCLGAGEAATGISDLLVAAMREDGVDEAQARQRCWMF
                     DSKGLVVQSRTDLAEHKLPYAHDHAPVTDFLGAVKALRPTAIIGVGAVPNSFTEAVVR
                     EMSRINERPVVFALSNPTSKAECTARQAYEWSDGRALYASGSPFDPVTLADGRHFVPR
                     QGNNSYVFPGLGLALVLVGASRVTDAMFLTAARTLAACTTEADLAQGSLYPPLAGVRE
                     VSARIAVAVAELAFRDGLAQIDHPEDLAALVHREMYDPRY"
     gene            complement(3214..3690)
                     /gene="bfr"
                     /locus_tag="VD737_02930"
     CDS             complement(3214..3690)
                     /gene="bfr"
                     /locus_tag="VD737_02930"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HXW09542.1"
                     /translation="MKGDVDVIAHLNKILYNELTAINQYFLHARMLKNWGLDRLGEHE
                     YHESIDEMKHADKLIERILFLEGLPNLQTLGKLLIGENLKEVLECDLRLEYAGHPDLK
                     AAIAHCEQVGDYVSRELFEDILESEEEHIDFLETQLQLIERVGIQNYTQSQMKSPS"
BASE COUNT          672 a         1501 c         1553 g          706 t
ORIGIN      
        1 ttccgcaccg gcagccaatg cggcgcggac gacgtgacga tgtgcctggc ggggctgccg
       61 gacttccggc aggagcaggc gggcgtcacc gagttcctga acggcacgat ccgttacctg
      121 cgcgacaacg ttccgggact cgacggcctg cgcatggaca cgatgaagca cgtcggcgac
      181 gcgtactggc ggcagttctt cgcgaccggc ggagtggcgg cgcccgccac gctctggacg
      241 gtgggcgaga tcttctcgag cgacgtcgcg caggtcgcga agtacgtcga tgcgctcggc
      301 tcgccttccg cattcgactt tccgctcaag ttcgccgtga ccgacagcct cggcaagggg
      361 cagagcaccc ggcgcctcgc agacctgttc gctcgcgatg ccacgtacga cgatccgacg
      421 cggctggcga ccttcctcga caaccacgac gtctggcggt tcacgtccga ggcggaggcg
      481 gcgggcgcca ccgcagccga ggccgaccgc cggctcgaca tggcgctgac gctgctctac
      541 gccgcgcgcg gcatccccgt cgtctactac gggaccgaga tcgcgatgcg tgggcgcggc
      601 gactcctaca gcctgcccct cggccagtcg agccgcgagg acatgcagtt ccagcggctc
      661 gccgaatcga ccttcgacga gcgcctggcc gcgctcgcgc aggcgcgcac ctcgcgtgcg
      721 gcgttgcgtc gcggctcgca gcgcacgctg gcggcgccgg gcgcgacctg cacggcgccg
      781 tcctcgagcc tgagcccgtc ggccgatttc ggcgatgcgc tgtacgtccg cggctcgttc
      841 gatgactggg cgaacccgcc cgcgacggcc aaccgcttcg cgaacctcgg cagtcgccag
      901 tacctggccg tggcggaact gcccgccggc cagatcgagt tcaaggtcgc ggccgccgac
      961 tggacgcccg agttttccaa cacgacgcag gcgaccgtgc cgggcgtgcc gatcaccctg
     1021 agcacgaact cgggcgccgg cacgaacagc cggcttcaag ccggcacgtc cgggtgctac
     1081 gagttcgcgc tgaatgcgac cagcaccagc gcgccggtgc tgacggtctc ggcacggagc
     1141 acggcggccg agccggacgt gtttgcgttc gtgcgcacgc tcgcgggcga aagcagcgtc
     1201 gtctacgtgg tcaacgccga gcgcgcgggc gtcgacctgg ccgcgctcgc gggcggcggc
     1261 atcgacgtcg gcgggatctt cgcggacggc gcggccctga cggaaatcac cggaaacgca
     1321 acggccgccc aggcgttgcg cgtcgccggc ggacggctgg tcgggacgct gccggcgctc
     1381 acggccgtgc tgctcgccgc aaactgagcg tccgtctgcc tctgccgcgg ggcgcgagag
     1441 gcaaggatca gtagcgcggg tcgtacatct cgcggtggac gagcgccgcg agatcctccg
     1501 ggtggtcgat ctgcgcgaga ccgtcgcgga acgccagttc ggcgaccgcg accgcgatac
     1561 gcgcggacac ctcgcgcacg cccgcgagcg gcggatacag gctgccctgc gccaggtctg
     1621 cttccgtcgt gcacgcggcg agcgtgcgcg ccgcggtcag gaacatcgcg tcggtcacgc
     1681 gcgatgcgcc gaccagcacc aacgcgagcc cgagccccgg gaacacgtac gaattgttgc
     1741 cctgccgcgg cacgaagtgc cgtccgtctg ccagcgtcac cggatcgaag gggctgcccg
     1801 aggcatacaa ggcgcggccg tccgaccact cgtaggcctg ccgtgccgtg cactccgcct
     1861 tcgacgtcgg gttcgacagc gcgaacacca ccgggcgctc gttgatccgt gacatctcgc
     1921 gtacgaccgc ctcggtgaag ctgttcggga cggcgcccac gccgatgatg gcggtcggcc
     1981 gcaacgcctt gacggcgccg aggaagtcgg tgaccggcgc gtgatcgtgc gcgtacggca
     2041 gtttgtgctc ggcgaggtca gtgcggctct ggacgaccag gcccttcgag tcgaacatcc
     2101 agcagcgttg ccgtgcctgg gcttcgtcga cgccgtcctc gcgcatcgcg gcgacgagca
     2161 ggtccgagat gcccgtggcg gcctcgcccg cgccgaggca caggaaccgc tggtcacgca
     2221 tgcgctggcc ggtcacccgc agcgacgaca ggatccccgc cacggtgacc gcggccgtcc
     2281 cctggatgtc gtcgttgaag cagcagatgc ggtcgcgata gcggcgcagc agtcggaacg
     2341 cgttgtggtt cgcgaagtcc tcgaactgca ccacgatgcc cgggaacact tcctgcgacg
     2401 cgaccatgaa ctcctcgacg agctcgtcgt atgccgcgcc cgtgaggcgc cgctggcgga
     2461 ggcccaggta gagcgggtcc tcgagcagcg ccgcgtcgtt ggtgccgacg tcgagcatga
     2521 cgggcaggca cttccacggg tggagtccgg cgcacgctgt gtacagcgag agcttgccga
     2581 tcggaatgcc catgccgttg gcgccaaggt cgcccaggcc gaggatgcgt tcgccgtcgg
     2641 tgacgacgat catcgcggcc tcgcggtgcg gccagttgcg catgacctgc gcgatgcggc
     2701 cgcggtcggt cgcgctgacg aacaacccgc gcgggcgacg gtagatgtgt ccgaactgct
     2761 ggcaggcgag gccgaccgtc ggcgtgtaga tgatcggcat catctcgtcg gggtggtcgg
     2821 tgacgacgcg atagaacagc gcctcgttcc ggtcgagcag gctcgacagc atgatgtact
     2881 tcgacagcgg cgtctgcagc cggcggaagt tctcgagcgc gcgcgcgacc tgctcgtcct
     2941 gggtgcagac gtgcgcgggc agcaggccgc gcagtccgag cacgtcccgc tcctgctcgg
     3001 tgaagcccgt gcccttgttc agcgtcgggt cgcgcagcag cgcggcgccg cggggaaacc
     3061 actcgggcag gggcggaagc gggcgcttgt gcatcggcgt gtccgggatc gaagacacgc
     3121 gcgatgctag tgggcgcgac ggggccgtca acgcggaaag tccctagctc acccttggcc
     3181 cgcgcgcgcg tcacgcgcgc ggacggcgcc tcctcaggag ggcgacttca tctgcgactg
     3241 ggtgtagttc tggatgccga cccgctcgat cagctgcagt tgcgtctcga ggaagtcgat
     3301 gtgctcttcc tccgactcga ggatgtcctc gaagagctcg cgcgagacgt agtcgcccac
     3361 ttgctcgcag tgggcgatcg cggctttgag gtcgggatgt cccgcgtact cgagccgcag
     3421 gtcgcactcg aggacttcct tgaggttctc gccgatcagc agcttgccga gggtctgcag
     3481 gttcggcagg ccctcgagga acaggatccg ctcgatgagc ttgtcggcgt gcttcatctc
     3541 gtcgatcgac tcgtggtact cgtgctcgcc gagccggtcg agtccccagt tcttcagcat
     3601 gcgcgcgtgc aggaagtact ggttgatcgc ggtcagttcg ttgtagagga tcttgttgag
     3661 atgagcgatg acgtcgacgt cgcccttcat ggcgtgactc cgaggtgcag aagaaaaggg
     3721 ccgccgaagc ggcccggaat caggaagcct gaggggatgg cttctcggaa tcaagcttgc
     3781 cgcagtcgag catctcgcgt gcgcagggct cgcaggctcc gcagcaggtg gccacgccgg
     3841 tgcacgactg aagctgctcg aaggagcgtg cgccctcgag gatcgcttgt tgcaaccggg
     3901 tctcgctgat gccgtggcaa acgcagacgt acatctgatg cacctcgctc tcggacggaa
     3961 tcctaggggg attttgaccg gaatgcaaat gattcgcatt taggtaattt ccacgatacc
     4021 gacgctgctc cccgcagtcg cccccgcctg ccggaggacg tccggtcggg gcaggcttct
     4081 ccgcgaccgc cggattgccg cctggcccga actggtgcgg gccgtcgcac ggggcctgcc
     4141 ggcgcgaggc gttgcccacc gaccggctgc cggagcgcat gcggcggcgc ctcgagcacc
     4201 tgctcgggat tccgttcggc gcgatcgtca ccaccagttc cgagacgctg ctacgcgagg
     4261 tcgccgccat gcgcgcgcga ggcggaggtc ggaaaatggg gacactccgg atttcccgtt
     4321 aattggggac actcctgatt tcgacctcgc gtaactgccc tttttcctcg gcacttgcgc
     4381 cacgacggtg cactgcggcc ggacgtcgcc tttcctcgcg agagcggtct cg
//
LOCUS       DASPFR010000117         4232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1377220, whole genome shotgun sequence.
ACCESSION   DASPFR010000117 DASPFR010000000
VERSION     DASPFR010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377220"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4203)
                     /locus_tag="VD737_02935"
     CDS             complement(<1..4203)
                     /locus_tag="VD737_02935"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HXW09543.1"
                     /translation="MPGTIPAERRQIIDAIARRARQRRRDRESTPLPLAEFVQRYFRG
                     VGEDDLRLREPAAFAASAAAHLGFGRVRRAGEPLVRVYNPDERRDGWTSPCTIVEVVT
                     DDMPFLVDSLAMVLDDAGLAIQVMVHPVLQVVRDGRGRLVELREEAGDNSVAESWQHV
                     AVTRIGDETRVEALRRRILGTLEDVRLAVSDWPAMRDRARLLAREVAAGVPGVRRQEE
                     VETCEFLEWLADNHFTFLGYREYELERGRTTDRLVPVARSGLGLLRAGEGRPKPAATE
                     LVGELRRKAREVALLIVTKANSVSTVHRSTYLDYVGVKTFDARGNVVGERRFIGLFTS
                     STYSTSPREIPLLRHKVQRIVDHFGISATSHDGKAIMHVLETFPRDELFQASVAELVR
                     TVRGIVNLYERRRVRVFLRRDPYRRFFSCLLFVPRDRYNTQVRERIERILIDEFQGVD
                     LESQIQMSESALARLHMLVRTDPAQEVFADVERIEARISDALRTWADHLREELTERLP
                     APEADALATRFLTAFPGAYQEDVRPQEAIRDLQELAALPDAPGALGMELRLGEAARGA
                     LHLRLYRRGDPVAMSDMLPLLENFDLRILNERPYRVASADGARLWIQDLEVRHAGGRT
                     LDPVQSGKRFEDAFFAVQHGLAESDGFNRLVLAAGLDWRQTLVLRAVCRYLLQTGIAF
                     SQKYMEEVLARNPEIAARLVWTFESRFDPELKAATRNAQVGAFAEEIAEALDQVTSPD
                     DDRILRAFRAVIMATLRTNHYQRTAVGEPKPYLSFKLDPSKLPDLPKPRPMFEIWVYS
                     PRVEGVHLRMGKVARGGLRWSDRREDFRTEILGLMKAQNVKNTLIVPVGAKGGFVPKQ
                     IAPGATREEVQREGTECYRWFIRGLLDLTDNVVGYDVIAPERTVRYDADDPYLVVAAD
                     KGTATFSDTANALAAEYGFWLGDAFASGGSAGYDHKKMGITAKGAWECVKRHFREMGI
                     DTQSQDFTVAGIGDMAGDVFGNGMLLSEHIRLVAAFNHQHIFLDPEPDAARSHAERRR
                     LFELPRSAWTDYDTRLISKGGGVYPRNAKEIALSTQARAMLGLEAAKATPAEIIRAIL
                     KLPVDLLWNGGIGTYVKASWEVNSAVGDRTNDAVRVDGRDLRCKVVGEGGNLGFSQLG
                     RIEYSLQGGRINTDFVDNSGGVDCSDHEVNIKILLKAVQERTSLTTAQRNRLLAQMTD
                     DVARLVLRDNYLQSQAISLMQAKAPERLGEHSHFIRSLEREGLLDRALEYLPTAEDIE
                     DRRRANQGLVRPELALMVSYAKIALNTQLIQSDVPEDPYLSQELDRYFPSRLASRYGE
                     VLGSHRLRREIIVTATTNSIVNRMGPTFVARTQQDTGADAATVARAYSIAREAFDVRD
                     LWAAIEALDNEVASAVQYDMMHD"
BASE COUNT          620 a         1452 c         1460 g          700 t
ORIGIN      
        1 gtcgtgcatc atgtcgtact gcacggcaga ggccacctcg ttgtcgagcg cctcgatcgc
       61 cgcccagaga tcgcgcacgt cgaaggcctc cctcgcgatc gaatacgcgc gagccacggt
      121 cgctgcgtcg gcgcccgtgt cctgctgcgt acgcgcgacg aacgtcgggc ccatccggtt
      181 gacgatgctg ttcgtcgttg cggtgacgat gatctcccgc cgcagccggt ggctgccgag
      241 cacttcgcca tacctgctcg ccaggcggct cgggaagtag cggtcgagct cctggctcag
      301 gtacgggtct tcaggcacgt ccgactggat cagctgcgtg ttgagcgcga tcttggcgta
      361 gctcaccatg agcgcgagct ccggccgcac caggccctgg ttcgcgcgac ggcggtcttc
      421 gatgtcctcc gccgtgggga ggtactcgag cgcgcggtcg agcaggcctt cgcgctcgag
      481 tgagcggatg aagtgcgagt gttcgccgag ccgctcgggc gccttcgcct gcatcaggct
      541 gatcgcctgg ctctgcaggt agttgtcgcg cagcacgagc cgtgcgacgt cgtcggtcat
      601 ctgcgccagc aggcgattgc gctgggccgt ggtgagcgag gtacgctcct ggacggcctt
      661 gagcaggatc ttgatgttga cctcgtggtc cgaacagtcg acgccgcccg agttgtccac
      721 gaagtcggta ttgatccgcc cgccctgcag cgaatactcg atgcgcccga gttgcgagaa
      781 cccgaggttg ccaccttcgc ccacgacctt gcagcgcagg tcgcggccgt ccacgcgcac
      841 ggcatcgttg gtgcggtcac cgaccgcgga gttcacctcc cacgatgcct tgacgtacgt
      901 gccgatgccg ccgttccaca gcaggtccac gggcagcttc aggatggccc ggatgatctc
      961 ggccggagtg gccttcgccg cctcgaggcc gagcatcgcg cgagcctgcg tcgacagcgc
     1021 gatttccttg gcgttgcgcg gatagacgcc gccgcccttc gaaatcagcc tggtgtcgta
     1081 atcggtccag gccgatcgcg gcagttcgaa caggcgcctg cgctccgcgt ggctgcgcgc
     1141 ggcatcgggt tcgggatcga ggaagatgtg ctggtggttg aatgcggcga cgaggcgtat
     1201 gtgctccgac agcagcatgc cgttgccgaa cacgtcgccg gccatgtcgc cgatgcccgc
     1261 caccgtgaaa tcctgcgact gcgtgtcgat gcccatctcg cggaagtgcc gcttgacgca
     1321 ctcccacgcg cccttcgccg tgatgcccat cttcttgtgg tcgtagcccg cggaaccgcc
     1381 cgacgcgaag gcgtcgccga gccagaagcc gtattcggcc gcgagcgcgt tggcggtgtc
     1441 cgagaacgtc gccgtgccct tgtcggccgc gacgacgagg tagggatcgt ccgcgtcgta
     1501 acgcaccgtg cgctctggcg cgatcacgtc gtagccgacc acgttgtcgg tgaggtcgag
     1561 caggccgcgg atgaaccagc gatagcactc ggtgccttcg cgctgcacct cctcgcgcgt
     1621 cgcgccgggc gcgatctgct tcggcacgaa gccgcccttg gcgcccaccg gaacgatcag
     1681 cgtgttcttg acgttctgcg ccttcatcag gccgaggatc tcggtgcgga agtcctcgcg
     1741 ccggtccgac cagcgcagcc ctccgcgcgc gaccttgccc atccggaggt gcaccccctc
     1801 gacgcggggc gagtagaccc agatctcgaa catcgggcgc ggcttcggca ggtcgggcag
     1861 cttcgacggg tcgagcttga acgacaggta cggcttgggc tcgcccacgg ccgtgcgctg
     1921 gtagtggttg gtccgcaggg tcgccatgat gacggcgcgg aaggcccgca ggatgcggtc
     1981 gtcgtcgggg ctcgtgacct ggtcgagcgc ttcggcgatc tcttcggcaa atgccccgac
     2041 ctgcgcgttg cgcgtggcgg ccttcagttc cgggtcgaac cgcgactcga acgtccagac
     2101 cagccgcgcg gcgatctcgg gattgcgcgc cagcacttcc tccatgtact tctggctgaa
     2161 tgcgatgccg gtctgcagca ggtagcggca gaccgcgcgc aggaccaggg tctggcgcca
     2221 gtccagcccg gcggccagca ccagccgatt gaacccgtcg ctctcggcca ggccgtgctg
     2281 cacggcgaag aacgcgtcct cgaagcgctt gcccgactgc acggggtcga gcgtcctgcc
     2341 gcccgcgtgc ctgacctcga ggtcctggat ccacagccgc gcgccgtcgg ccgacgcgac
     2401 ccgatacgga cgctcgttga ggatccggag gtcgaagttc tcgagcagcg gcagcatgtc
     2461 cgacatcgcg acagggtcgc cgcgccggta cagccgcagg tgcagcgcac cgcgtgccgc
     2521 ttcgccgagg cgcagttcca tgccgagcgc gccaggcgcg tcgggcaggg ccgcgagttc
     2581 ctgcaggtct cggatcgctt cctgcgggcg gacatcctcc tggtaggcgc ccggaaacgc
     2641 agtcaggaag cgggtcgcga gcgcatcggc ctcgggcgcg ggtaaccgct cggtgagctc
     2701 ctcgcgcagg tggtccgccc aggtgcgcag cgcgtcgctg atgcgcgctt cgatccgctc
     2761 gacgtcggcg aagacctcct gtgccggatc ggtgcggacc agcatgtgca ggcgcgcgag
     2821 cgcggactcc gacatctgga tctgcgactc caggtcgacg ccctggaact cgtcgatcag
     2881 gatgcgctcg atgcgttcgc gcacctgggt gttgtagcgg tcgcgtggca cgaacagcag
     2941 gcacgagaag aagcgacggt acggatcgcg gcgcaggaac acgcgcacgc gccggcgctc
     3001 gtagaggttg acgatgccgc gcaccgtccg gacgagttcc gccacgctcg cctggaacag
     3061 ctcgtcgcgc gggaacgtct cgagcacgtg catgatcgcc ttgccgtcgt ggctcgtagc
     3121 ggagatcccg aagtgatcga cgatccgctg caccttgtgg cgcagcagcg ggatctcgcg
     3181 cgggctcgtg ctgtaggtgc tcgacgtgaa gagtccgatg aagcggcgtt cgccgacgac
     3241 gttgccgcgg gcgtcgaacg tcttgacgcc gacgtagtcc aggtacgtgg aacggtgcac
     3301 ggtcgagacc gaattggcct tggtcacgat cagcagcgcc acctcgcggg ccttgcgccg
     3361 cagttccccc acgagctccg tggcggccgg cttcgggcgg ccttcgcccg cgcgcagcag
     3421 cccgagcccc gaccgcgcca ccgggaccag ccggtccgtc gtgcggccgc gctccagctc
     3481 atactcgcgg tagcccagga acgtgaagtg gttgtcggcg agccactcga ggaactcgca
     3541 ggtctcgact tcttcctgcc tgcgcacgcc ggggacgccc gcggcgactt cacgcgcgag
     3601 caacctggca cgatcgcgca tcgccggcca gtccgacacc gcgagtcgca cgtcttccag
     3661 cgtgccgagg atgcggcggc gcagtgcctc gacgcgtgtc tcgtcgccga tgcgcgtcac
     3721 ggccacgtgc tgccacgact ccgcgaccga gttgtcgccg gcctcttcgc gcagctcgac
     3781 cagacggccg cggccgtcgc gtacgacctg cagcaccggg tgcaccatga cctggatcgc
     3841 gagcccggcg tcgtcgagca ccatggcgag cgaatcgacc aggaacggca tgtcgtcggt
     3901 cacgacctcg acgatggtgc agggagacgt ccagccgtcc cgccgttcat cggggttgta
     3961 gacccgcacg aggggctcgc cggcacgacg cacccgaccg aacccgaggt gcgctgcggc
     4021 cgacgcggcg aacgctgccg gctcgcgcag gcgcaggtcg tcctcgccga cgccgcggaa
     4081 gtagcgctgc acgaattcgg ccagcggcaa cggcgtggac tcacggtcgc gccggcgctg
     4141 gcgcgcgcgt ctggcgatcg cgtcgatgat ctgcctgcgt tcggcgggga tggttcctgg
     4201 catgaagcgg gtccttcgaa gtctctggtc tc
//
LOCUS       DASPFR010000118         4669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1012520, whole genome shotgun sequence.
ACCESSION   DASPFR010000118 DASPFR010000000
VERSION     DASPFR010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012520"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1069)
                     /locus_tag="VD737_02940"
     CDS             complement(68..1069)
                     /locus_tag="VD737_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09544.1"
                     /translation="MKAAATLRLALPWLHRAIVEARATGSLPALDSLSWLVGRGTAAP
                     APDDDWREWLLGVDAHALAEFRRWPAGPCLAAATGARPDSAPAWGVAQPVHLVAGMDH
                     VRLGPLADALPADAEAEQLADTVRAHFAGDVLDLAGYRDHAWLVRCVEAVECTTHDPA
                     SLAGLDIHDYLPAGPDGARMRSRMNEIQMVLHEHPVNERRANTRAAAINALWLWGFGR
                     LEAVPGKLDAAGRWTLLTDDLWLRAFWQVHGGAERPLGTAGTPDGDTLVAMTQPPTAE
                     PAEALTEVDSSLLARICRSLQDGALHELKVHDGARVHTLDRWSRWRLWRRAARPADL"
     gene            1132..1500
                     /locus_tag="VD737_02945"
     CDS             1132..1500
                     /locus_tag="VD737_02945"
                     /inference="COORDINATES: protein motif:HMM:NF016326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HXW09545.1"
                     /translation="MKFHLDAPGGLNLVRGYGPGQLRVGERTHHSSVILTATAIIEGW
                     RPASVQDLTAADLEPVLGLAPEVVLLGTGAHQQFPDSAILRILYEQRIGVEIMDTSAA
                     CRTFNVLVSEGRSVAAALIV"
     gene            complement(1508..2179)
                     /locus_tag="VD737_02950"
     CDS             complement(1508..2179)
                     /locus_tag="VD737_02950"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HXW09546.1"
                     /translation="MTMVDPTRHNGIGMTSARTRERLVARLREQGIVNPEVLERIRGV
                     PRHVFVDEALASRAYEDTALPIGHGQTISQPYIVARMTQSLVEAGKPRKVLEVGTGCG
                     YQTAVLAPLVTTLYSVERIAALQQRARKVLRDLRIGNVHMKHGDGFEGWAAYAPFDGI
                     IVAAASYSVPPALVEQLADGGRLVIPVGTDREQQLLRITRRGERLEKETLGPVTFVPL
                     LQGLA"
     gene            complement(2176..2937)
                     /gene="surE"
                     /locus_tag="VD737_02955"
     CDS             complement(2176..2937)
                     /gene="surE"
                     /locus_tag="VD737_02955"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HXW09547.1"
                     /translation="MRILVSNDDGYRAEGLQHLVDALSPLAAITVVAPDRNRSGASHS
                     LTLDVPLRVIEFGERRYYVQNGTPTDCVHLAISGLFPDEVDHDMVVSGINDGPNLGDD
                     VLYSGTVAAAIEGRFLGLPAIALSLVLRDGRHFATAARVAAELLMRLQRSPLHATMIL
                     NVNVPDLPYEQLKGYRTTRLGQRHRSEPVVPASDPRGRPVYWVGPAGEGADAGEGTDF
                     HAVANGYVSVTPLQVDLTRYAALEEIGDWLSGRPE"
     gene            3046..3591
                     /locus_tag="VD737_02960"
     CDS             3046..3591
                     /locus_tag="VD737_02960"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HXW09548.1"
                     /translation="MSDPSEPPPDDDVHAFRRAVADVRPLTPRSRVPEPGARPGPRAR
                     FARAEREEVLRESLVTPLDPAALETGDHLAFKRPAVREEVLRKLKRGQFAVEAEIDLH
                     GLGRHAAHEALREFLNDCVRRGLRCVRVIHGKGLRSGPGGPVLKHVVDHWLRRIENVV
                     AFASARPVDGGTGAVYVLLRK"
     gene            complement(3628..4326)
                     /locus_tag="VD737_02965"
     CDS             complement(3628..4326)
                     /locus_tag="VD737_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXW09549.1"
                     /translation="MRRTSVVGSLGSMPRGPRSIHPGVCYHVINRGNHRRRLFHRSAD
                     YAAFVALIQKARDLVPIELFAVCLMPNHFHVVLRAAERDAVGRWMHWLLTTHAQRYNR
                     HHGIVGRVWQGRYKAFPIERDDHLLTVLRYVERNAVRAHLVRRPRDWHWCSASWRRGP
                     SSRRALLTAPPVALPADWDDLVEQPLETAAVEQLRACVNRQQPYGSDAWIDGSGECAG
                     FHRARPRGRPPCRK"
     gene            complement(4415..>4669)
                     /gene="truD"
                     /locus_tag="VD737_02970"
     CDS             complement(4415..>4669)
                     /gene="truD"
                     /locus_tag="VD737_02970"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HXW09550.1"
                     /translation="ASPASGPALEVEREALAAHAASIDLLAREGLDHERRPLRLPVRE
                     FDWQFEGEDQLVLRFTLPRGTFATAVLHELLTDAWDAGEE"
BASE COUNT          682 a         1671 c         1645 g          671 t
ORIGIN      
        1 gcgggtaacg ccgcctgcag ctccgcacgc gggtcgcggc ggacgatgcg tcgcgccggt
       61 gtcgacatca aaggtccgcc ggccgggcgg cgcgacgcca caggcgccag cgcgaccaac
      121 ggtcgagcgt gtgcacgcgc gccccatcgt gcactttcag ttcgtgcagt gccccgtcct
      181 gcagcgagcg gcagattcgc gcgagcaggc tcgaatcgac ttcggtcagg gcctcggcgg
      241 gttcggcggt cggcggctgc gtcatcgcga cgagcgtgtc gccgtcgggc gtgccggccg
      301 tcccgagcgg ccgttccgcg cctccgtgca cctgccagaa cgcgcgcagc cacaggtcgt
      361 ccgtcagcag cgtccaccgg ccggcggcat cgagcttgcc cggcacggcc tcgagccgac
      421 cgaaccccca cagccacaac gcgttgatgg ccgcggcccg cgtgttcgcg cgtcgctcgt
      481 tgaccggatg ctcgtgcagc accatctgga tctcgttcat gcggctgcgc atgcgtgcgc
      541 cgtccgggcc ggcgggcagg tagtcgtgga tgtcgagtcc ggcgagcgac gccgggtcgt
      601 gcgtggtgca ctcgaccgcc tcgacgcagc ggacgagcca cgcgtggtcg cgataacccg
      661 ccaggtcgag cacgtcgccc gcgaagtgcg cgcggacggt gtccgccagc tgctcggcct
      721 ccgcgtccgc cggcagcgcg tccgcgagag gaccgaggcg cacgtggtcc atcccggcga
      781 cgaggtggac cggctgcgcg accccccagg ccggcgcgct gtcgggccgg gcgccagtgg
      841 ccgccgccag gcagggccct gccggccacc gacggaattc ggccagggcg tgcgcgtcga
      901 cgccgagcag ccactcgcgc cagtcgtcgt ccggagccgg cgcggcggtg ccgcggccga
      961 ccagccacga cagcgagtcg agcgcgggca gcgagcccgt cgcgcgcgcc tccacgatcg
     1021 cgcggtgcag ccacggcagt gcgaggcgca acgtcgccgc ggccttcatc gctgcggcgc
     1081 cgtgcccgcg acccggcggc gatccgccgc gtcctgcggt atggttctgc gatgaaattc
     1141 cacctcgacg caccgggcgg cctgaatctc gtgcgcggct acggacccgg ccaactgcgc
     1201 gtcggcgagc gcacgcacca ctcgagcgtg atcctcaccg cgaccgcgat catcgaaggc
     1261 tggcgtcccg cgtcggtgca ggacctcacc gccgccgacc tcgagcccgt gctcggactc
     1321 gcccccgaag tcgtcttgct cggcaccgga gcgcatcagc agttccccga cagcgcgatc
     1381 ctgcgaatac tgtacgagca acggatcggc gtggaaatca tggacacctc ggccgcctgc
     1441 cgcacgttca acgtgctggt cagcgagggc cgctcggtcg cggccgcgct gatcgtctga
     1501 ggccagttca ggcaaggccc tgcaacagtg gcacgaacgt caccgggccg agggtctcct
     1561 tctcgagccg ctcgccacgc cgcgtgatcc gcagcaactg ctgctcgcgg tccgtgccga
     1621 cggggatgac cagccggccg ccgtcggcga gctgctcgac gagcgccggc ggcacggaat
     1681 acgacgccgc ggcgacgatg atgccgtcga acggcgcata tgcggcccag ccttcgaagc
     1741 cgtcgccgtg cttcatgtgc acgttgccga tgcgcaggtc gcgcagcacc ttgcgggcgc
     1801 gttgctggag cgcggcgatg cgctcgacgc tgtagagcgt cgtcacgagc ggcgccagca
     1861 ccgcggtctg gtaaccacag cccgtgccga cctcgagcac cttgcgcggt ttgccggctt
     1921 ccaccagcga ctgcgtcatc cgcgccacga tgtacggctg cgagatcgtc tgcccgtgcc
     1981 cgatcggcag cgcggtgtcc tcgtacgcgc ggctcgcgag tgcttcgtcg acgaacacgt
     2041 ggcgcgggac gccgcggatg cgctcgagca cttccgggtt gacgataccc tgctcgcgca
     2101 gccgcgcgac gagccgctcg cgcgtcctcg ccgaggtcat gccgatgccg ttgtgtcgcg
     2161 tcggatccac catcgtcatt cggggcgccc gctcaaccag tcgccgattt cctcgagggc
     2221 cgcgtaccgc gtcaggtcga cctgcagcgg cgtgaccgaa acatacccgt tggcgacggc
     2281 gtggaaatcc gtgccttccc cggcatccgc gccctcgccc gccggcccga cccagtagac
     2341 cgggcgaccg cgcgggtcgg aggccggcac gacgggctcc gagcggtggc gctggccgag
     2401 ccgcgtggtc cggtagccct tcaactgctc gtagggcagg tccgggacgt tgacgttgag
     2461 gatcatcgtg gcgtgcagcg gcgagcgctg cagccgcatg agcagttcgg cggcgacgcg
     2521 ggcggcggtc gcgaaatgcc ggccgtcgcg cagcaccagc gacagcgcga tggcaggcag
     2581 gccgaggaag cgtccctcga tggcggcggc caccgtaccc gaatagagca catcgtcgcc
     2641 gaggttcggg ccgtcattga tgccggagac caccatgtcg tggtcgacct cgtcgggaaa
     2701 gagcccggag atcgcgaggt gcacacagtc ggtcggcgtg ccgttctgga cgtagtagcg
     2761 gcgctcgccg aactcgatca cgcgcagcgg cacgtcgagg gtcagcgagt ggctcgcccc
     2821 gctgcggttg cggtcgggcg ccacgacggt gatcgcggca agcggggaca gcgcgtcgac
     2881 gaggtgctgc aaaccctcgg cgcgatagcc gtcgtcgtta ctgaccagga ttctcaccag
     2941 gcgtgggacc gtgtcgttgt ctgggcccca gcagtggcgg ggttggccac gaacgcgacg
     3001 ccactatact ggaatccccg gcggcctcgg aagcgaactc gcaccatgtc ggatccctcc
     3061 gagccacctc ccgacgacga cgtccacgcc tttcgccgcg cggtcgcgga cgtgcggccg
     3121 ctcacgccgc gctcgcgtgt gccggagccc ggtgcaaggc cgggtccacg cgcacgtttc
     3181 gcgcgcgccg aacgcgagga ggtgctgcgc gagagcctcg tgaccccgct cgatccggcc
     3241 gcgctcgaga cgggcgatca cctcgcgttc aagcggccgg cggtgcgtga agaggtgctg
     3301 cgcaagctca agcgcggcca gtttgcggtc gaggcggaga tcgacctgca cgggctcgga
     3361 cgtcacgccg cccacgaagc gctgcgcgaa ttcctgaacg actgcgtgcg tcgcggcttg
     3421 cgctgtgtac gcgtgatcca cggcaagggc ctgcgctccg ggccgggcgg tccggtgctc
     3481 aagcacgtcg tcgatcactg gctgcgccgg atcgagaacg tcgtcgcgtt cgcgagcgcg
     3541 cggccggtcg acggcggcac cggcgccgtc tacgtcctcc tccggaaata gggacactcc
     3601 gaatatccgc cgaattgggg acactcctca tttacggcac ggtggtcggc ctcgaggccg
     3661 ggctctgtgg aatcccgcgc actcgccgga gccgtcgatc caggcgtccg atccataggg
     3721 ctgttggcga ttgacgcagg cacgaagctg ctccaccgcc gccgtctcga gcggctgctc
     3781 gacgagatcg tcccagtccg caggcaacgc cacgggcggc gccgtcaaca gcgccctgcg
     3841 cgaggacggt ccgcgacgcc acgacgcgct gcaccagtgc cagtcgcgcg gccgccgcac
     3901 caggtgcgcg cgcacggcgt tgcgctcgac gtaacgcagc accgtcagca ggtggtcgtc
     3961 gcgctcgatc gggaacgcct tgtaccgccc ctgccacacg cggccgacga tgccgtgatg
     4021 acggttgtag cgctgcgcgt gggtcgtcag cagccagtgc atccaccgtc cgaccgcgtc
     4081 acgctcggcc gcgcgcagca cgacgtggaa gtggttcggc atcaggcaga cggcgaacag
     4141 ctcgatcggc acgaggtcgc gcgccttctg gatcagcgct acgaacgctg cgtagtccgc
     4201 cgaacgatgg aacaggcggc gtcgatggtt gccacggttg atcacgtggt agcagacgcc
     4261 cggatggatc gatcgcggtc ccctcggcat ggacccgagg ctgccgacga ccgaggtcct
     4321 gcgcaccgcg caaatccggg caggatgcgc ggaaattcgg agtgtcccca cttaacggga
     4381 aattcggagt gtcccctatt cctcggagtg tcccctattc ctcgccggcg tcccaggcgt
     4441 cggtgagcag ctcgtgcagc acggcggtcg cgaacgtgcc gcgaggcagc gtgaagcgca
     4501 gcaccagttg atcctcgccc tcgaattgcc agtcgaactc gcgcaccggc aatcgcagcg
     4561 ggcggcgttc gtgatcgagt ccctcgcggg cgagcaggtc gatcgaggcc gcgtgcgccg
     4621 ccagcgcctc gcgctcgacc tcgagcgccg ggccgctcgc cggcgaggc
//
LOCUS       DASPFR010000119         5345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_36540, whole genome shotgun sequence.
ACCESSION   DASPFR010000119 DASPFR010000000
VERSION     DASPFR010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36540"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VD737_02975"
     CDS             complement(<1..277)
                     /locus_tag="VD737_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXW09551.1"
                     /translation="MNKTIYVTGTNTGVGKTVATAAALRLARRHGLRCAGLKPVATGG
                     ERSVPGAPLRNGDALELQSAGSMPLDYDTVNPFCFEPAIAPHLAARDA"
     gene            complement(343..2067)
                     /gene="bioH"
                     /locus_tag="VD737_02980"
     CDS             complement(343..2067)
                     /gene="bioH"
                     /locus_tag="VD737_02980"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR02072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HXW09552.1"
                     /translation="MTAVYVETRGEGPDLVMLHGWALHGGSWGPWLDGLAARNRLHLV
                     DLPGHGRSPWPDHALSLHELARAVSPSVPAGAAILGWSLGGLVALELARQRGADVAAL
                     VLLATTPRFLVEHDWPSGMEPATLQGFAAGLAGDYRRTVANFLALQTWGDEHATAALR
                     SLRASLAAHGEPDPRALRAGLEILREADLRDALSQVAAPALVIAGEHDRITPAAAGRA
                     LAARLPSARYREIAKAGHAPFLSHGDEVLREVEGFLSSLPRPAPSAPGAAARRLPGAF
                     AARRATADDPFALDGRRVRHSFSDAARTYDSAAALQARIRDELLERLDLLTMRPRTVV
                     DLGAGTGRGAAALRHRYREATVVALDNAPGMLVEAGRRQGLFRRFHRVGGDAAALPLQ
                     DATTDMIFSSLLLQWCNDPDRVLRECRRVLRPGGVLHLATLGPDTLTELRRAWQVADP
                     DHVHVSRFLDMHDVGDALVRAGFAEPVLDVERHTLAYGDARDLMRDLKAIGAHNATVG
                     RSRGLTGKGVLARMLAAYETARRDGRLPATYEVVFAQAWAPEHPVPGRPADEAGEVLV
                     PLSRLRRR"
     gene            complement(2064..3257)
                     /gene="bioF"
                     /locus_tag="VD737_02985"
     CDS             complement(2064..3257)
                     /gene="bioF"
                     /locus_tag="VD737_02985"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HXW09553.1"
                     /translation="MDGRLTADLAAMAASGRARRRRTVEGRSQGGVRMRLDGHEYLAF
                     CSNDYLGLGTDPRVVRAFQDAAGRWGVGAGASHLVSGHCTEHEALEDELAAYVNRPRA
                     VAFSTGYMANLAVAATLVGRGDTVFEDRLNHASLLDAGLATGARFVRYRHGDAAALRS
                     KLAKSPRGGTRLVMTDAVFSMDGDVAPLAELATACRAGGAELMVDDAHGFGLLGPDGA
                     GAVSEAGLGLDDVPVLMCTLGKAAGTFGAFVAGSTALVDSLVQRARTYLYTTASPPAL
                     AAATREALRVMREESWRREVVLAHVRAFRAGVAALGLTLLPSRTAIQPLVLGDEASAV
                     AASAALMDAGFLVPAIRPPTVPAGTSRLRFTFSAAHREHDVQRLLDALASLRLGGHVP
                     AEVTT"
     gene            complement(3276..4265)
                     /gene="bioB"
                     /locus_tag="VD737_02990"
     CDS             complement(3276..4265)
                     /gene="bioB"
                     /locus_tag="VD737_02990"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008305604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HXW09554.1"
                     /translation="MTPPDLARLGAVRTDWLFDEAHAVYAAPFADLLFHAQRVHRENF
                     VPNRVQVSTLQSVKTGACPEDCKYCPQSVRYDTGLEREELLQVDEVVAAAKAARAQGA
                     TRFCMGAAYRSPKARQLDQIKRMVTEVRALGLETCATVGMLTPEQARELKDAGLDYYN
                     HNLDTSREYYGEIVTTRRYEDRLETLQAVRDAGIRVCCGGILGMGESERDRVGLLHTL
                     ATLPQHPESVPINQLVQVPGTPLHGLAPLDPLELVRAIATARLLMPRSYVRLSAGRTD
                     MDDATQALCFLAGANSVFYGDRLLTTPNPGRSHDEDLFARLGIETEHAVHAEG"
     gene            4374..5117
                     /locus_tag="VD737_02995"
     CDS             4374..5117
                     /locus_tag="VD737_02995"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HXW09555.1"
                     /translation="MRRKVYRLLDRVAGVLLPPRCLLCGGPGAGRARDLCTACEADLP
                     WIDAACRRCGHPVEAPFVEACARCRDRPQPHRHCHAALHHEFPAAELVAALKYQGALA
                     HARVLGTLLADSLARHGVRREVELLVPMPLHASRLIERGFNQSLEVARFTADGLDVEV
                     DGRALRRLRATVPQVGLTRPQRADNVRGAFEADPARVAGRHVALIDDVVTTGATSAAA
                     ARALLAAGAATVDVWCVTRAASSDAQANR"
     gene            complement(5102..>5345)
                     /gene="ubiA"
                     /locus_tag="VD737_03000"
     CDS             complement(5102..>5345)
                     /gene="ubiA"
                     /locus_tag="VD737_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the conversion of 4-Hydroxybenzoate into
                     3-octaprenyl-4-hydroxybenzoate, as part of the ubiquinone
                     biosynthesis pathway; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HXW09556.1"
                     /translation="DRGIVAALQVGALALLVVAGMLSGLGSWYWLGLAVAAATAVHQH
                     YLIRHRDPGECFRAFLNNNLFGLAVFGGILLDYLFA"
BASE COUNT          757 a         1939 c         1938 g          711 t
ORIGIN      
        1 cggcgtcgcg ggccgccagg tgcggggcga tcgccggctc gaaacagaac gggttcacgg
       61 tgtcgtagtc gagtggcatc gacccggccg actgcagttc cagcgcgtcg ccgttgcgca
      121 gcggcgcccc ggggaccgac cgctcgcccc cggtggcgac cggcttcaac ccggcgcacc
      181 ggaggccgtg tcgccgcgcg aggcgcaacg ccgcggccgt cgccacggtc ttgcccacgc
      241 cggtgttggt tccagtcacg tagatcgtct tgttcaactt gatagggcgc ctgcttcccg
      301 ttgctttatg gaccgacctt catggtttgg tttataaaag catcaccgcc gccgaagtcg
      361 cgacagcggt acgagtactt cgcccgcctc gtccgccgga cgcccgggca caggatgctc
      421 cggtgcccag gcctgtgcga agacgacctc gtaggtcgca ggcaaccggc catcgcgcct
      481 cgcggtctcg tacgcggcca gcatccgcgc cagcacgccc ttgccggtca gcccgcgcga
      541 ccgccccacg gtcgcgttgt gggcgccgat cgccttgagg tcgcgcatca ggtcgcgcgc
      601 gtcgccgtag gcgagcgtgt ggcgctccac gtcgagcact ggctcggcga accccgcgcg
      661 gaccagcgcg tcgccgacgt cgtgcatgtc gaggaaccgg ctcacgtgga catggtcggg
      721 atcggcgacc tgccaggccc gtcgcagttc cgtcagcgtg tccggcccga gcgtcgcgag
      781 gtgcagcacg ccgccaggcc gcagcacgcg gcggcattcg cgcaggacgc ggtcgggatc
      841 gttgcaccac tgcagcagca ggctcgagaa gatcatgtcg gtcgtggcgt cctgcagtgg
      901 cagggccgcc gcgtcgccgc ccacgcggtg gaaccgccgg aacagcccct ggcgacggcc
      961 ggcctcgacg agcatgccag gagcattgtc gagcgcgacc accgtggcct cacgataacg
     1021 gtgccgcaac gcggcggcgc cgcgcccggt gccggcaccg aggtcgacca ccgtgcgggg
     1081 ccgcatcgtg agcaggtcga gccgctcgag cagttcgtcc cgaatccgcg cctgcagcgc
     1141 ggccgccgag tcgtaggtgc gagcggcatc ggagaacgag tggcggaccc ggcgaccgtc
     1201 gagcgcgaac gggtcgtccg ccgtggcccg acgtgcagcg aacgctccag gcaagcggcg
     1261 ggcagcggca cccggcgcgc tcggtgccgg tcgaggcagc gatgacagga acccctcgac
     1321 ttcgcgcagg acctcgtcgc catgcgacag gaacggcgcg tgccccgcct tggcgatctc
     1381 ccgataacgg gccgagggca ggcgggcggc gagggcccgg cccgcggcgg cgggagtgat
     1441 ccggtcgtgc tcgccggcga tcaccagcgc aggtgcggcg acctgcgaca gcgcgtcgcg
     1501 caggtcggcc tcccgcagga tctcgagccc ggcccggagc gcgcgcggat cgggctcccc
     1561 gtgcgcggcg agcgatgcac gcagcgagcg caacgccgcc gtcgcgtgct cgtcccccca
     1621 ggtctgcaag gccaggaaat tggcgaccgt gcgccggtaa tcgcccgcga ggccggccgc
     1681 gaagccctgc agcgtcgcag gttccatgcc cgacggccag tcgtgctcga ccaggaaacg
     1741 cggcgtcgtg gcgagcagca ccagcgccgc cacgtccgcg ccccgctgcc gcgcgagttc
     1801 gagcgccacg aggccaccga gcgaccagcc gaggatcgcg gcgcccgccg gcacgctggg
     1861 cgacaccgca cgcgccagct cgtggaggct gagcgcgtgg tccggccacg gactgcgacc
     1921 gtggccgggc aggtcgacca ggtgcaaccg gttccgggcc gccagtccgt cgagccaggg
     1981 accccagctg ccgccgtgca gggcccagcc gtgcagcatt accaggtccg ggccctcgcc
     2041 gcgcgtctcg acatatacgg ccgtcacgtc gtgacctcgg caggcacatg cccgcccagt
     2101 cgcaggctcg cgagcgcatc gagcaggcgc tggacgtcgt gctcgcgatg cgcagccgaa
     2161 aacgtgaagc gcaggcgtga cgttccggcc gggacggtcg gtggccggat ggccggcacc
     2221 aggaatcccg cgtccatcag cgcggccgac gcggcgacgg cgctggcttc gtcgccgagc
     2281 acgagcggct ggatcgccgt gcgcgaaggc agcagcgtca ggcccagcgc cgccacgccg
     2341 gcgcggaacg cgcgcacgtg cgcaagcacg acctcgcgtc gccacgactc ctcgcgcatc
     2401 acgcgcagcg cttcgcgtgt cgccgccgcc aacgcgggtg gcgacgccgt cgtgtagagg
     2461 tacgtgcggg ccctctgcac cagcgagtcg acgagcgccg tcgagcccgc gacgaaggct
     2521 ccgaacgtgc cggccgcctt gccgagcgtg cacatcagca cgggcacgtc gtccagcccc
     2581 aggcccgctt ccgacacggc gcctgcgcca tcggggccga gcaggccgaa accgtgcgcg
     2641 tcgtcgacca tcagttcggc gccgccggcc cggcaggccg tcgccagctc ggcgagcggc
     2701 gcgacgtcgc cgtccatgct gaacaccgcg tccgtcatca ccagccgcgt tcctccacgc
     2761 ggcgacttcg cgagcttcga gcgcaatgcc gccgcgtcgc cgtggcggta tcggacgaac
     2821 cgcgccccgg tggcgagccc cgcgtccagc agcgaagcat ggttgaggcg atcctcgaac
     2881 acggtgtcgc cgcgcccgac gagcgtcgcc gccacggcca ggttggccat gtagccggtc
     2941 gagaacgcca cggcgcgcgg gcggttcacg taagccgcga gctcgtcctc gagcgcctcg
     3001 tgctccgtgc agtggccgct gacgagatgc gacgcgccgg cgccgacgcc ccagcggccg
     3061 gcggcgtcct ggaatgcccg cacgacgcgc ggatcagtgc cgagccccag gtaatcgttg
     3121 ctgcagaacg ccaggtactc gtgcccgtcg aggcgcatgc gcacgccgcc ctgtgatctc
     3181 ccctcgacgg tgcgccgtcg tcgtgcccgt cccgaggcgg ccatggcggc gaggtccgcg
     3241 gtgagcctgc cgtccacgcg cgacacgacg ggccgtcaac cctcggcgtg gacggcgtgc
     3301 tcggtctcga tcccgagccg tgcgaacagg tcctcgtcgt ggctccggcc ggggttgggc
     3361 gtggtcagca ggcgatcgcc atagaagacc gaattggccc ccgccaggaa gcacagtgcc
     3421 tgcgtcgcat cgtccatgtc ggtgcgtccg gccgagagcc gcacgtacga gcgcggcatc
     3481 agcagtcgcg ccgtggcgat cgcgcggacg agctcgagcg ggtcgagcgg cgcgaggccg
     3541 tgcagcggcg taccgggcac ctgcacgagc tggttgatgg ggacgctctc cggatgctgc
     3601 ggcagagtcg ccagcgtgtg cagcaggccc acccggtccc gctcggactc gcccatgccg
     3661 aggatgccgc cgcagcagac gcggatgccg gcgtcgcgaa ccgcctgcag cgtctccagt
     3721 cggtcttcgt aacggcgcgt ggtgacgatc tcgccgtagt actcgcgcga cgtgtcgagg
     3781 ttgtggttgt agtagtcgag ccccgcatcc ttgagctcgc gcgcctgttc cggcgtcaac
     3841 atgccgaccg tcgcgcaggt ttcgaggccg agcgcgcgca cttcggtgac catgcgcttg
     3901 atctggtcga gctgccgcgc cttggggctg cggtacgccg cgcccatgca gaaacgcgtc
     3961 gcgccctgcg cccgggcggc cttggcggcg gcgacgacct cgtcgacctg cagcagttcc
     4021 tcgcgctcga gcccggtgtc gtagcgcacg ctctgcgggc agtacttgca gtcctccgga
     4081 caggcgccgg tcttgaccga ctggagcgtg ctcacctgca cgcggttggg cacgaagttc
     4141 tcgcggtgca cgcgctgcgc gtggaacagc aggtcggcga atggcgccgc gtagaccgcg
     4201 tgcgcttcgt cgaacaacca gtcggtgcgg acggcgccca gtcgggcgag atcgggcggg
     4261 gtcatcggaa atccctcgaa aggcctggga ttggcgacgt gcgccgccgc ggtcgccggt
     4321 gcagcctgga ggggccgcag tattcgtgcg gcccggacct ctgtcaacca aacgtgcgtc
     4381 gcaaggtata caggctgctg gaccgcgtcg ccggggtgct gctgccgccg cgctgcctgc
     4441 tctgcggcgg cccgggcgcg ggccgtgccc gcgacctgtg cacggcctgc gaggcggacc
     4501 tgccctggat cgacgccgcg tgccggcgct gcggtcatcc ggtggaggca ccgttcgtgg
     4561 aggcctgtgc gcggtgtcga gatcggcccc agccgcaccg gcactgtcat gcagcgctgc
     4621 accatgaatt tcccgcggcc gaactggtgg cggccctcaa gtaccaaggc gcactcgcgc
     4681 acgcgcgcgt gctcggcacg ctgctcgcgg acagcctcgc ccggcacggc gtgagacgcg
     4741 aggtcgagtt gctcgtgccg atgccgctgc acgcatcgcg actgatcgag cgcggcttca
     4801 accagtcgct cgaggtcgcg cgattcacgg ccgacgggct ggacgtcgag gtcgacggcc
     4861 gggcgctgcg gcgcctgcgc gcgaccgtcc cgcaggtcgg gctcacgcgc ccgcagcggg
     4921 ccgacaacgt gcgcggtgcc ttcgaggccg acccggcgcg cgtcgcagga cggcacgtcg
     4981 cgctcatcga cgacgtcgtg accactggtg cgacgagcgc tgccgcggcg cgtgccttgc
     5041 tcgccgccgg cgcggccacg gtcgacgtct ggtgcgtgac gcgggccgcg tcgagcgacg
     5101 ctcaggcgaa caggtaatcg agcaggatcc cgccgaacac cgccagcccg aagaggttgt
     5161 tgttcaggaa agcgcggaag cattcgccgg ggtcgcggtg gcggatcagg taatgctgat
     5221 gcacggccgt ggccgccgcc acggccaggc cgagccagta ccacgagccg agccccgaca
     5281 acatgccggc gacgaccagc agggcgaggg caccgacctg cagggcggcg acgatgcccc
     5341 ggtcg
//
LOCUS       DASPFR010000120         7078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_280811, whole genome shotgun sequence.
ACCESSION   DASPFR010000120 DASPFR010000000
VERSION     DASPFR010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7078
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280811"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VD737_03005"
     CDS             complement(<1..729)
                     /locus_tag="VD737_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXW09557.1"
                     /translation="MTREPWYVVVAKRAARGLYASWTLGTLAVLMTLAVLLAFVLPAL
                     AWRRSSTRFFARAWLGLAGLRVRTTGLETLPGGSCVLVANHSSYLDGVLMKAVLPPRF
                     SFVIKREAASMPVIGLLLTRIGAEFIDRHTPGGRQRDARRVVERAEQGHSLVFFPEGT
                     FDAAVGLKRFHLGAFVAAARGNVPVVPAVIHGARRALPSDTYVPRPGRLHVEVLRPVG
                     GRGEPPEQLRDEARSAILLRLGEPE"
     gene            935..1888
                     /gene="xerD"
                     /locus_tag="VD737_03010"
     CDS             935..1888
                     /gene="xerD"
                     /locus_tag="VD737_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HXW09558.1"
                     /translation="MSTAVKPSYHSRTRTAGGGASQETPGPIDRFLDAVWMERGLSPN
                     TLAAYRADLSALERWLDEHGVHLENAQRGDILSFMASRVQAGARPRSTARQLSSFRRF
                     YRYLVREGSLREDPTAQIAMPKVGRSLPKSLMEEEVEALLAAPAVSDPLGHRDRTMLE
                     VLYATGLRVSELVNLKLGQVNLNQGVLRIVGKGDRERLIPLGEEAVQWVQQFLQGPRV
                     EILLERQTDYLFPTRRGDRMTRQAFWHIIKRYARKAGIDKELSPHTLRHAFATHLLNH
                     GADLRVVQMLLGHSDLSTTQIYTHVARERLKDLHSQHHPRG"
     gene            2115..2945
                     /locus_tag="VD737_03015"
     CDS             2115..2945
                     /locus_tag="VD737_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HXW09559.1"
                     /translation="MHRTLLQAPIVAALMLFASAATTTAAAQPAAESRSQSKGTSRPS
                     NTVKAASSEIPAEVRTRVVAKLPGATPDDVSASPIPGLYEVTMGGLIAYVSEDGKYLV
                     SGSVYDLDTQMNLTASRRNAARAKALAAASESQMIVFGPQNAKMTVTVFTDIDCGFCR
                     KFHNQIADVNKAGVRVRYLMYPRTGPGTESWRKAEQVWCSADRRDALTRAKRGEPLKA
                     KACGDGAIKTQYELGSDLGVEGTPAIFTQTGDYIGGFLTPEELVSSVQASQKAALAAR
                     "
     gene            complement(3002..3325)
                     /gene="fdxA"
                     /locus_tag="VD737_03020"
     CDS             complement(3002..3325)
                     /gene="fdxA"
                     /locus_tag="VD737_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016138692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HXW09560.1"
                     /translation="MTFVVTENCIKCKYMDCVEVCPVDCFHEGPNMLVIDPDECIDCT
                     LCEPECPAEAIFSEEELPDGQQPFRQLNADLSKQWPVITEKKDAPPDAKEWDGRPDKL
                     PLLER"
     gene            complement(3378..3923)
                     /locus_tag="VD737_03025"
     CDS             complement(3378..3923)
                     /locus_tag="VD737_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXW09561.1"
                     /translation="MKFCSSCGQAVTLRTPEGDHLPRHVCDACGVVHYQNPKLVVGCV
                     PEYEGRVLICKRAIEPRYGFWTVPAGFMENGETTQEGARRETLEEALADVEIGSLLAV
                     VHVLHADQVHVMFRARMHEPSFGATAESLEVRLCDEAEIPWHDIAFRSVDFALRRYFD
                     DRRRGVEGHHFAALDYRKPAG"
     gene            complement(3956..4042)
                     /locus_tag="VD737_03030"
     tRNA            complement(3956..4042)
                     /locus_tag="VD737_03030"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4006..4008),aa:Leu,seq:caa)
     gene            4237..4869
                     /locus_tag="VD737_03035"
     CDS             4237..4869
                     /locus_tag="VD737_03035"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXW09562.1"
                     /translation="MPAFPDIDVLATGLLDRARAGEEHALEQIYVAYERPVRTLALRL
                     LAHRAAAEDVAQDVFVDLITRLHQYEGRGSFAGWVRSIAVSRCLMHLRSPWQRSRRWL
                     GRQLGETPDGHGHSADEWPAADGPDASLAGQDGDTIDLERALARLGGTARAVVWLHDV
                     EGYTHAEIGALFGATASFSKSQLARAHERLRAELQSEGVGSPCMPVSSIY"
     gene            4842..5480
                     /locus_tag="VD737_03040"
     CDS             4842..5480
                     /locus_tag="VD737_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09563.1"
                     /translation="MHARVEHLLSLRDGHPVDVAVRAHVQRCARCAAALADATELPAR
                     LRALPPAAADQRGWRGVRERLASRRRADAVRARLGRVAAVASIAVIAVAVAWRVAESP
                     GLHSPDPTVRIATPLSAEEAVALDRVLQLRSQSAALEDALAVIGDRPAVQRAGTTLPI
                     DTIEAQVQWIDHRLSAGAGDDAIEAERLWRERVDAMNTLVRLRYVELQPVAM"
     gene            5503..6549
                     /locus_tag="VD737_03045"
     CDS             5503..6549
                     /locus_tag="VD737_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXW09564.1"
                     /translation="MTMTKTDSKTARRAARVRRHVIAVAWAGFAALGTLPVATAHGAE
                     QAPQERAALEKQLKDAQARLDAAARDVADLSSQLYGGDARHDVLKYRQGVPGPRGAML
                     GINIGGSEQARPDGVEIMSVSPSGPAEQAGLRKGDVITAVDGEPLRKSNDMSAGRRLV
                     EHLRGVEPGQVVKLDYLRDGKKQTASVTTVAAEPPMMRIVREHLPMLEGLDLPFDVDA
                     LTGSPGRGFRSLELVPVTPKLGQYFGTDKGLLVVRAPAAAGAGLEEGDVIMTIGGRTP
                     ENPRHAFRILGSYQPSESVKVEVLRQRKRMTVEMKVPAAPEAPAFRPAPRPAVPAVPP
                     VAPAPAPRSDSVSS"
     gene            6566..>7078
                     /locus_tag="VD737_03050"
     CDS             6566..>7078
                     /locus_tag="VD737_03050"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HXW09565.1"
                     /translation="MRAPMRRADFHYHLPDELIARHPAPERSASRLLHLDGRTGALVD
                     RRFRELPGLLRAGDLLVFNDTRVVPARLHGHKRDSGGRVEVMLERLLGGPSALVQLRA
                     SKPPREGSTIVFGDGQALVVERREDEFWRVRFEVNAHEAFERHGEVPLPPYVGRREEP
                     ADRERYQTVYA"
BASE COUNT         1098 a         2492 c         2457 g         1031 t
ORIGIN      
        1 ttctggttca ccgagcctga gcaggatcgc tgaccgcgcc tcgtcgcgca gttgctccgg
       61 cggctcgccg cgcccgccga ccggccgcag cacttcgacg tgcagccggc ccggacgcgg
      121 cacgtaggta tcgctcggca acgcccgtcg cgcgccgtgt atgaccgccg gaacgaccgg
      181 cacgttgccg cgcgccgcgg cgacgaacgc gcccaggtgg aagcgcttca ggccgaccgc
      241 cgcgtcgaac gtgccctccg ggaagaacac cagcgagtga ccttgctcgg cgcgctcgac
      301 cacgcggcga gcgtcccgct ggcggccgcc gggcgtgtgc cggtcgatga actcggcacc
      361 gatccgcgtc agcagcaggc cgatcaccgg catcgacgcg gcttcccgct tgatcacgaa
      421 gctgaaccga ggcggcagca ccgccttcat gagaacgccg tcgaggtagc tcgagtggtt
      481 cgccaccagc acgcaagagc cacccggcag cgtctcgagg ccggtcgtgc gtacgcgcag
      541 ccccgccagg ccgagccacg cccgcgcgaa gaagcgcgtg ctcgagcgcc gccacgcgag
      601 cgccggcagc acgaaggcca gcagcactgc gagcgtcatc agcacggcca gcgtcccgag
      661 cgtccaggac gcatacagtc ctcgcgccgc acgctttgcg acgacgacgt accagggttc
      721 acgggtcacg gatggtgagt gccttctcgc ccgagcattc gaagcgcgcg cgacgcacca
      781 tcaggcgctc gtcggagtgc tcgtgtcggt ggcgcgaaac attctgtgaa tcagttccgg
      841 ttatgttaac gcgccccagg atctgtgagc gatgtcacga cgaccccgga tgaccgcctc
      901 gcatactgcg cgctacgcaa gaccgcgagg ttccatgagc actgcagtga agccgagcta
      961 tcactcccgt acgcgcaccg ccggcggcgg ggcgtcgcag gagacgcccg gccccatcga
     1021 ccggttcctc gacgcagtct ggatggagcg cgggctgtcg ccgaacacgc tcgccgcgta
     1081 ccgcgccgac ctgagcgcgc tcgagcggtg gctcgacgag cacggcgtgc acctcgagaa
     1141 cgcccagcgt ggcgacatcc tctcgttcat ggcctctcgc gtccaggccg gcgcgcggcc
     1201 gcgctccacg gcgcgccagc tctcgagttt ccgccggttc tatcgttacc tggtccgcga
     1261 aggctcgttg cgcgaagacc cgaccgcgca gatcgcgatg cccaaagtgg ggcgttcgtt
     1321 gccgaagtcg ctgatggagg aggaagtcga ggccctgctg gccgctcctg ccgtcagtga
     1381 cccgctcggg catcgcgatc ggaccatgct ggaggtgctc tatgccacag gcctgcgcgt
     1441 ctcggaactc gtcaacctga agctcggcca ggtgaacctg aaccagggcg tgctgcggat
     1501 cgtcggcaag ggtgaccgcg agcggttgat tccgctcggc gaggaagccg tgcagtgggt
     1561 gcagcagttc ctgcaggggc cgcgtgtcga gatcctgctc gagcgccaga ccgactatct
     1621 gttcccgacg cggcgcggcg atcgcatgac gcgccaggcc ttctggcaca tcatcaaacg
     1681 ctacgcgcgc aaggccggca tcgacaagga gctgtcgccg cacacgctgc gccacgcgtt
     1741 cgcgacgcac ctgctgaacc acggtgccga cctgcgcgtc gtgcagatgc tgctcggtca
     1801 cagtgacctg tcgaccacgc agatctacac gcatgtcgcg cgcgagcgtc tcaaggacct
     1861 gcactcgcag caccatccgc gcggctgatg tgggcccctg cggggctccg tgggggcatc
     1921 gtcggcatcg ggtatcgcac cggtctgcta gacttcgcgc cgctcgcgcg aggccggccc
     1981 gttgaacccg gaacgacgcc gtttgtccta gaggcagccc gacgcgcgct gtcgtatccc
     2041 gcgatgccct gcgtccgcgg gcccgtcacc cgggtcgctt aacgatgtcg aacgcccaca
     2101 ggaaacgtcc acgcatgcat cgcacgctct tgcaggcccc gatcgtcgcc gccctgatgt
     2161 tgttcgcgtc cgccgcgacg acgacggccg ccgcccagcc ggcggcggaa tcccggtcgc
     2221 agtccaaggg cacgagccgc ccgtccaaca ccgtcaaggc cgcgtcgtcg gaaataccgg
     2281 cggaggtgcg gacgcgcgtc gtcgccaagc tgcctggggc caccccggac gacgtcagcg
     2341 cctcgccgat cccggggctc tacgaagtca cgatgggcgg gctgatcgcg tacgtcagcg
     2401 aggacggcaa gtacctcgtg agcggcagcg tctacgacct cgacacccag atgaacctga
     2461 ccgcatcgcg tcgcaacgcg gcgcgcgcca aggcgctcgc ggcggcgtcg gaaagccaga
     2521 tgatcgtgtt cgggccgcag aacgcgaaga tgacggtcac cgtgttcacg gacatcgatt
     2581 gcggcttctg ccgcaagttc cacaaccaga tcgccgacgt gaacaaggcc ggcgtccgag
     2641 tccgctacct gatgtacccg cgcaccggcc ccggcaccga atcctggcgc aaggccgagc
     2701 aggtgtggtg ttcggccgac cggcgcgacg cgctgacgcg cgcaaagcgt ggcgaaccgc
     2761 tcaaggcgaa ggcctgcggc gacggcgcga tcaagaccca gtacgaactg ggctcggatc
     2821 tcggcgtcga aggcacgccg gcgatcttca cgcagaccgg agactacatc ggcggcttcc
     2881 tgactccgga agaactcgtc agctcggtgc aggcttcgca gaaggcggcg ctcgccgccc
     2941 gctagcgatc ctcccggccc gcgccacgac gtgcgcggtg acgttggcct ggtccgcctc
     3001 gtcagcgttc gagcagtggc agcttgtccg gccgaccgtc ccactccttg gcatcgggcg
     3061 gcgcgtcctt cttttcggtg atcaccggcc actgcttgga aagatccgca ttcagctgcc
     3121 ggaacggctg ctggccgtcg ggcaattcct cttccgagaa gatcgcttcg gccgggcact
     3181 ccggctcgca gagcgtgcag tcgatgcact cgtccgggtc gatgaccagc atgttgggac
     3241 cttcgtggaa gcagtccacc gggcagactt ccacgcagtc catgtacttg cacttgatgc
     3301 agttttccgt gacgacgaag gtcatcggct actccgggcg agacggctga aaccgggacg
     3361 ggaaacgtga atcgtaccta accggccggc ttcctgtaat ccagtgccgc gaagtggtgg
     3421 ccctcgaccc cgcgccgccg atcgtcgaaa taacgcctga gcgcgaaatc cacgctgcgg
     3481 aacgcaatgt cgtgccaggg gatctcggcc tcgtcgcaga gccgcacctc gaggctttcc
     3541 gcagttgcgc cgaagctcgg ctcgtgcatg cgcgcgcgga acatgacgtg gacctggtcg
     3601 gcgtgcagca cgtgcacgac cgcgagcagc gagccgatct cgacgtcggc gagtgcctcc
     3661 tcgagcgtct cgcgccgtgc accttcctgc gtggtctcgc cgttttccat gaagccggcc
     3721 gggacggtcc agaagccgta tcggggctcg atcgcacgct tgcagatcag gacgcggcct
     3781 tcgtattccg gcacgcagcc caccacgagc ttcgggttct ggtagtggac cacgccgcag
     3841 gcgtcgcaga cgtggcgagg caagtggtcg ccttcgggcg tgcgcagggt gacggcttgg
     3901 ccgcagctgg agcagaactt catcgatgca acgttggacc gccggagcag ggcgttggtg
     3961 ccgggagagg gaatcgaacc ctcacgatgt cgccaccgcc ggattttgag tccggtgcgt
     4021 ctaccagttc cgccatcccg gccaggaagg gctgcgaagc ttaaccgatt gtcgcgcaac
     4081 cggcaggcgg gccgccgcgg ccgcagggct cgccatggcg cgtgcgtgtc gtggaactcg
     4141 gcttcggcgc gcgaggtcaa ccggaccgcc ggccagggcc acggccgact ccgtggccgc
     4201 gaccttttcc agcgctcgcg catctgttgg agcaacatgc ccgcgtttcc cgacatcgac
     4261 gtcctcgcca ccggcctgct cgacagggcc cgggccgggg aggaacacgc gctcgaacag
     4321 atctatgtcg cgtacgagcg accggtgcgc acgctcgcgc tgcgcctgct ggcccaccgc
     4381 gccgccgcgg aggacgtcgc ccaggacgtc ttcgtcgacc tgatcacccg gctgcaccag
     4441 tacgagggtc gcggctcgtt cgcgggctgg gtgcgcagca tcgcggtctc gcggtgcctg
     4501 atgcacctgc ggtcgccctg gcagcgcagc cggcgctggc tgggccggca gctcggcgag
     4561 acgcccgacg gtcacggtca cagcgcagac gaatggcccg ccgcggacgg accggacgcc
     4621 agcctggcgg gccaggacgg cgacacgatc gacctcgagc gcgcgctcgc gcgcctcggc
     4681 ggcaccgcgc gcgccgtcgt ctggctgcac gacgtcgagg gctatacgca cgcggagatc
     4741 ggtgcgctgt tcggggccac cgccagtttt tccaagtcgc agctcgcgcg cgcgcacgaa
     4801 cgcctgcgtg ccgagctgca atccgagggg gtcgggtcac catgcatgcc cgtgtcgagc
     4861 atctactgag cctgcgcgac ggccatccgg tcgacgtcgc cgtccgcgcg cacgtccagc
     4921 gctgtgcgcg ctgtgcggcg gcgctcgccg acgcgaccga gctgccggcg cgtctgcgcg
     4981 ccctgccacc ggccgcagcg gaccagcgtg gctggcgcgg cgtgcgcgag cggctcgcat
     5041 cgcgacgacg agccgacgcg gtgcgggcgc gcctgggccg cgtcgccgcc gtcgcctcga
     5101 tcgccgtgat cgcggtcgcc gtggcgtggc gagtggccga gtcgccgggc ctgcactcgc
     5161 ccgacccgac ggtgcggatc gcgacgccgc tcagcgccga agaggccgtg gcgctcgatc
     5221 gtgtcctgca gctgcggtcg cagtcggcgg cgctcgagga cgcgctggcc gtgatcggcg
     5281 accgcccggc cgtacagcgt gccggcacga ccctgcccat cgacaccatc gaggcgcagg
     5341 tgcaatggat cgaccaccgc ctgtccgctg gcgccggcga cgacgcgatc gaggccgagc
     5401 gactctggcg cgagcgtgtc gatgcgatga acacgctcgt acggctgcgc tacgtcgagc
     5461 tccagccggt cgcgatgtaa aggacagagg atcaacctga ggatgaccat gacaaagacc
     5521 gattcgaaga ccgcgcggcg cgccgcgcga gtccgccgtc acgtgatcgc cgtcgcctgg
     5581 gccggcttcg cggcgctcgg aacgctgccg gtggcgaccg ctcacggcgc cgagcaggcg
     5641 ccgcaggagc gcgcggcgct cgagaagcag ctcaaggacg cgcaggcgcg gctcgatgcc
     5701 gccgcgcgtg acgtggcgga cctgagcagc cagctctacg gcggcgacgc gaggcacgac
     5761 gtgctcaagt accgccaggg cgtgcccgga ccgcgcggcg cgatgctcgg gatcaacatc
     5821 ggtggcagcg agcaggcgcg tcccgacggc gtggagatca tgagtgtcag cccttcggga
     5881 ccggccgagc aggcgggcct gcgcaagggc gacgtcatca cggcggtgga cggcgagccg
     5941 ctgcgcaagt ccaacgacat gagcgcgggt cggcggctgg tggagcacct gcggggcgtg
     6001 gaacccgggc aggtcgtgaa gctcgactac ctgcgagacg gcaagaagca gacggcctcg
     6061 gtgacgaccg tggcggcgga gccgccgatg atgcgcatcg tgcgcgagca cctgccgatg
     6121 ctcgagggcc tcgacctgcc gttcgacgtc gacgccctga cgggcagtcc gggtcgcggt
     6181 tttcgttcgc tcgaactcgt gccggtcacg ccgaagctcg gccagtactt cggcacggac
     6241 aagggtctgc tcgtggtgcg cgctccggcg gccgccggcg ccgggctcga ggagggtgac
     6301 gtgatcatga cgatcggcgg ccgcacgccc gagaacccgc gtcatgcgtt ccggatcctc
     6361 ggttcgtacc agccctcgga gtcggtaaag gtcgaagtcc tgcgacagcg caagcgcatg
     6421 acggtcgaga tgaaggttcc ggcggctccc gaggctcctg ccttccgacc ggcgcctcgg
     6481 ccggcggtgc cggcggtgcc gcccgtcgcc ccggcgccag caccgcgcag cgacagcgtc
     6541 tcttcctgac ggccggtggg gtagcatgcg cgcccctatg cggcgcgcgg atttccacta
     6601 ccacctgccg gacgagctga tcgcgcgtca tccggcgccc gagcgcagcg ccagtcgcct
     6661 gctgcacctc gacggccgga cgggcgcgct cgtcgaccgg cggttccgcg aactgcccgg
     6721 gctgctgcgc gccggagacc tgctggtctt caacgacacg cgcgtcgtcc cggcgcggtt
     6781 gcacgggcac aagcgcgaca gcggcggtcg ggtcgaggtc atgctcgagc gactgctcgg
     6841 cggcccgagc gcactcgtgc agctgcgcgc gagcaagccg ccgcgcgagg gttcgacgat
     6901 cgtattcggc gacggacagg ccctggtcgt cgaacgtcgc gaggacgagt tctggcgcgt
     6961 gcgcttcgag gtgaacgccc acgaggcgtt cgagcgccat ggcgaggtgc ccctgccacc
     7021 ttacgtcggt cgacgcgagg agccggctga ccgcgagcgt taccagaccg tctacgcc
//
LOCUS       DASPFR010000121         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1623602, whole genome shotgun sequence.
ACCESSION   DASPFR010000121 DASPFR010000000
VERSION     DASPFR010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623602"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VD737_03055"
     CDS             <1..393
                     /locus_tag="VD737_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HXW09566.1"
                     /translation="KQVVANARAMVVVFLERGINVVSGGTDNHLFLLDFSAKSFTGKD
                     ADAALGRAHITVNKNSVPNDPRPPMVSSGIRIGTPAVTTRGFKEAEVSQLAHWMCDVL
                     ADVNDEAVIARTRESVQQICRRFPVYGS"
     gene            472..912
                     /gene="nrdR"
                     /locus_tag="VD737_03060"
                     /pseudo
     CDS             472..912
                     /gene="nrdR"
                     /locus_tag="VD737_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006878018.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
     gene            1064..2203
                     /gene="ribD"
                     /locus_tag="VD737_03065"
     CDS             1064..2203
                     /gene="ribD"
                     /locus_tag="VD737_03065"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005415257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HXW09567.1"
                     /translation="MADSYSVADHEHMARALRLAALGRYSAAPNPAVGCVLTDSAGRV
                     VGEGWHRRTGEPHAEVLALAASQGRERGGTAYVTLEPCAHHGRTPPCVDAVLAAAPGR
                     VVVAMEDPNPRVAGQGIRRLREAGVPVHVGLLAAAAAELNRGFVSRMTRARPWVTVKL
                     GSSLDGRTALADGSSKWITGPHARADVQRLRAQASAVLTGIGTVLADDPALTVRDPRF
                     PLHGRRPLRVIFDARGQLPPRARLLNDDHDTLVLTSRAGAAELQAHGVHGQLHLELEL
                     LPVDAHGRLDPAVALARLAERQCNEVLVESGARLAGSFLSAGLVDELVVYLAATVLGQ
                     DARPSFSLPQPIAALEHRARFDYHDVRTVGGDLRVTLRPAREEGV"
     gene            2203..2832
                     /locus_tag="VD737_03070"
     CDS             2203..2832
                     /locus_tag="VD737_03070"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HXW09568.1"
                     /translation="MFTGIVQGVGTVRAIVPREGDVTIVFDTGDVALDGVEPGGSIAV
                     NGVCLTATTRDRSSFTADVSRETLSLTTLGRWREGTRVNVEKALLAGQALGGHYVTGH
                     VDGVAEVVSRHDDARSVRMAFRVPRELARYVARKGSICIDGVSLTVNGVDDDRFDVNL
                     VPHTLEVTTLGALEPGSRVNVEVDIIARYVERMSGAAAAGQPPPTATPC"
BASE COUNT          419 a         1007 c         1040 g          406 t
ORIGIN      
        1 aagcaggtgg tcgcgaacgc acgcgcgatg gtcgttgtgt tcctcgagcg cggcatcaac
       61 gtcgtctcgg gcggcaccga caaccacctg ttcctgctcg acttcagcgc caagtcgttc
      121 accggcaagg acgccgacgc cgcgctcggg cgggcgcaca tcacggtcaa caagaactcc
      181 gtgccgaacg acccgcggcc gccgatggtc tcgagcggca tccggatcgg tacgccggcg
      241 gtcaccacgc gcgggttcaa ggaagccgag gtcagccagc tcgcgcactg gatgtgcgac
      301 gtgctcgccg acgtcaacga cgaagccgtg atcgcgagga cgcgagagtc cgtgcagcag
      361 atctgccggc gcttccccgt gtacggaagc tgaccggctt cgggcatcac gggcagttcg
      421 tcagacggac cttccaccgg cggactcacg atcaggcgga gcgcgaggcc gatgtactgt
      481 ccgttctgct cgttcgagga gaccaaggtc atcgactcac gcctgtcggc ggaggggcag
      541 caggttcgac ggcggcgcga atgcctgcgg tgcggtgagc gcttcacgac cttcgaatcg
      601 gccgagctcg tgatgccgcg cgtggtcaag agcgactcga gccgcgaacc gttcgacgag
      661 gcgaaactgc gctccgggat gatgagggcg ctcgagaagc gcccggtcgc gagcgagcgc
      721 gtggacgcgg ccgtgaacca cgtctgtcac aagctgcgca cgctcggcga gcgtgaggtg
      781 ccggcgcgcc tgatcggaga actcgtcatg gacgagctgc accagctcga cgaggtcgcc
      841 tacgtgcgct tcgcgtcggt ctaccgcagc ttccaggacg tcgacgcgtt ccgcgaggag
      901 atcgagcggc tccggcaccg ccgtccgcgg tcggccgaag acgtgcagct ggcgctctgg
      961 tcgtccggtg gcgaagcagc gtcgccggcg ccggcgcaga ccccgacggc cgctggccgc
     1021 gagccgggca gcgggcagaa gccatagcgc agtcatctcc agcatggctg attcatattc
     1081 cgtcgccgat cacgagcaca tggcacgggc gctgcggctc gcggcgctcg gtcgctactc
     1141 ggcggcgccg aatcccgccg tgggctgcgt gctgaccgat tccgccggac gcgtggtcgg
     1201 cgaaggatgg catcggcgga ccggcgagcc gcacgccgaa gtcctcgcat tggcggcatc
     1261 gcaggggcgc gagcgcggcg gcaccgccta cgtcacgctc gagccctgcg cgcaccacgg
     1321 acgcacgccg ccgtgcgtcg acgcggtact cgctgccgct cccgggcggg tcgtcgtcgc
     1381 gatggaagac ccgaatcccc gggtcgcggg gcagggcatc cggcgtctgc gtgaagcggg
     1441 cgttccggtc cacgtcggcc tgctcgccgc cgcggcggcc gaactcaatc gcggattcgt
     1501 gtcgcgcatg acgcgcgcgc ggccgtgggt gacggtcaag ctcggctcga gtctcgacgg
     1561 acgcaccgcg ctggccgacg gctcgagcaa gtggatcacc gggccgcacg cgcgcgccga
     1621 cgtgcagcgg ctgcgcgcgc aggcatccgc ggtgctgaca ggcatcggca cggtgctggc
     1681 agacgatccc gcgctcacgg tccgcgatcc gcgctttccg ctgcacggcc gccggccact
     1741 gcgcgtgatc ttcgacgcgc gcggccagct gccgccgcgc gcccggcttc tcaacgacga
     1801 ccacgacacc ctggtgctga ccagccgggc gggagcggcc gagctgcagg cgcacggcgt
     1861 ccacggccag ctgcacctcg aactcgagct gttgccggtc gacgcacacg gccgtctgga
     1921 cccggctgtc gcgctggcgc ggctcgcgga gcgacagtgc aacgaggtgc tggtcgagtc
     1981 gggcgcccgc ctcgcgggaa gcttcctgtc ggccggactg gtcgacgaac tggtggtcta
     2041 cctggccgcc accgtgctcg gccaggacgc gcggccgtcg ttctcgctgc cgcagccgat
     2101 cgccgctctc gagcaccggg cgcgattcga ctatcacgac gtgcgcaccg tcgggggcga
     2161 tctgcgtgtc acgctgcggc cggcgcggga ggagggcgtc tgatgtttac cgggatcgtg
     2221 cagggcgtcg ggacggtgcg cgcgatcgtg ccgcgcgagg gtgacgtgac gatcgtgttc
     2281 gacaccgggg acgtggcgct cgacggcgtg gagccgggcg gcagcatcgc cgtcaacggc
     2341 gtctgcctca cggcgacgac gcgagaccga tcgagcttca cggccgacgt gtcgcgcgag
     2401 acgctgtcgc tcacgacgct cggccgctgg cgcgagggga cgcgagtgaa cgtcgagaag
     2461 gccctgctcg cggggcaggc gctcggcggc cattacgtca ccggccatgt cgacggtgtc
     2521 gccgaggtcg tctcgcgcca cgacgacgcc cgctccgtcc gcatggcgtt ccgcgtgccg
     2581 cgggaactcg cgcgctacgt ggctcgcaag ggttcgatct gcatcgacgg cgtgagcctg
     2641 accgtgaacg gcgtggacga cgaccgcttc gacgtcaacc tggtgccgca caccctcgaa
     2701 gtgacgaccc tcggggccct cgagcccggc agccgcgtca acgtcgaggt ggacatcatc
     2761 gcgcggtacg tcgagcgcat gagcggcgcc gccgccgcgg gccagccgcc ccccactgcg
     2821 acaccctgct aaactgcgcg tcgccgcgcg cgctccagcc gcggattcgg ct
//
LOCUS       DASPFR010000122        11973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_402391, whole genome shotgun sequence.
ACCESSION   DASPFR010000122 DASPFR010000000
VERSION     DASPFR010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11973
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402391"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VD737_03075"
     CDS             complement(<1..551)
                     /locus_tag="VD737_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXW09569.1"
                     /translation="MTNARILVVDDENDIRELVREILTEEGYTVETAGTAAEARAACA
                     RQAPELVLLDIWMPDTDGISLLREWQQSQGLNMPVVMMSGHGTVETAVEATRLGAVDY
                     VEKPLSLAKLLRTVQRALDEGRRRERATRTLVPPLIAPVGRSRLMRDLRDQVKQIAPH
                     EAPVLVLGEPGTGREAFARYIHT"
     gene            complement(548..2812)
                     /locus_tag="VD737_03080"
     CDS             complement(548..2812)
                     /locus_tag="VD737_03080"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXW09570.1"
                     /translation="MDAPTLRRTASAITVGVGGLLWLAAILFMAQTAQNSEQFSRLHP
                     WILMINIAGLAVLVGLLATKLAQLIRDWRGHVIGSRLKARMVWIFGVLATLPILLVYF
                     FAVQFLSKGIDSWFNVEIRRSLDDALVLSRSALEVRMREHLADSTMVADELSVMRGPR
                     MAGLLETLRRQVGALELVVANDSGSIIAVSKGFSDDLVPEAVPDEVLLQVRQGQPFVS
                     LEPQQDGGYLIRTAVPIGVSLPGEQRLVVQGLYPVAERLGALADTVQAAYKQFGEKDY
                     LRQPLKASFILTLTVVLLLSLLAAWYGAIFTAQRLVQPIQDLVQGTRAVAHGDLDTKL
                     PLTSHDEMGFLVHSFNDMTKRLRRAREESSRAQQAVETERANLAVILARLSTGVISLE
                     RDWRIRTANRAASMILGEDIETQVGAGFDAMGAEPQGLARQFAEACRQRCEAGATEWR
                     EQFTFVAGTSRRILMCACTPLPGEGDAPGGYVLVFDDITGMLQAQREAAWGEVARRLA
                     HEIKNPLTPIRLSAERMRHRLLPAMSDPKDAQMLERATETIVQQVEAMKEMVNAFSEY
                     ARAPRLEMSTVDLNRMVTEVSDLYRAQGAMRSIRLKVQLDPAIDSVVADAGRLRQLLN
                     NLLTNAVEALEGQGQGSISVTTQRALHGETEVAKIAVEDDGPGFQREMIGQVFDPYVT
                     TKVKGTGLGLAIVRKIVEEHGGHIEADNRPSGGARVCVDLPLHDAGSERGRGNGNSSG
                     ASGRGTSEPRRERA"
     gene            complement(2794..3357)
                     /locus_tag="VD737_03085"
     CDS             complement(2794..3357)
                     /locus_tag="VD737_03085"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HXW09571.1"
                     /translation="MARWTLPLLLCVIATLAAPERASAQASLTLSDVRIGVDDGVYEL
                     DARLDLRLPDDARRAVESGLTLRLNYEIVIDRVRRYMLDAEVAALVQRYEVTYHALSQ
                     RWLVRNLNTGEQDDFGSLDTALARISELRGLPIIDTALLPAGPEYQGRIRAVLDLSTA
                     PDAFGWLLFWADDWSAESDWKTWTLRP"
     gene            complement(3409..4734)
                     /gene="rsmB"
                     /locus_tag="VD737_03090"
     CDS             complement(3409..4734)
                     /gene="rsmB"
                     /locus_tag="VD737_03090"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF008149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HXW09572.1"
                     /translation="MNTAAPARLRAEAARIVAEVLAKGRSLDDLLAAEQDEGSARGLK
                     RSLCYGTLRWHFRLAAVLNALAARPPEQLDPRLRALLEVGLYQLMSGETAPHAAVSET
                     VSAARALGFDKAAGFVNAVLRRFQREQAALLHAVDRDLSIRTAHPRWFVDALRQDRGD
                     AAPAVLEANNAHPPLWVRINRRRTSIEEAANVLNTAGFTTTRHPWASDALQVEPPTDV
                     RSLPGFAEGRLSVQDAAAQLAIDLLEPAPGERILDACAAPGGKTCHVLERTDGAVDLT
                     ALDVSATRIERVRENLVRLGLEASIRVADVAAVAEWWDSRPFDRVLLDVPCSATGVIR
                     RHPDIKILRRARDIPALARRQAELLEAAWQVVRPGGRLLYTSCSVLAAENERVVAGFL
                     ARTRGARDLTPALTAAWPARPDGHGPGYQVLPGESGMDGFYYACLAKSL"
     gene            complement(4731..5741)
                     /gene="fmt"
                     /locus_tag="VD737_03095"
     CDS             complement(4731..5741)
                     /gene="fmt"
                     /locus_tag="VD737_03095"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010117311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HXW09573.1"
                     /translation="MTPALRIVFAGTPEFSVPALDALQAAGHSIVAVYTQPDRPAGRG
                     RALASSPVKRRAEQLGLVVEQPATLRTPEAQARLASHAPEVMVVVAYGLILPQPVLDI
                     PRLGCINIHASLLPRWRGAAPIQRALLAGDAQTGISIMQMDAGLDTGPELLRRELAIG
                     SRETGGELHDRLAPLGAAAIVEALAGVASGTLIARAQPEAGATYAAKIRKDEARIDWS
                     RPTLEIDRLVRAFNPWPVAETRLDGEQVRIWEARPAGKWGHSYLSAGNQGHSGFPDGK
                     VEMSPGTVLDDPGRLVVATGDGALELLRLQLPGRKPVSAADVLNARDLRGRRFASTVV
                     AA"
     gene            complement(5738..6280)
                     /gene="def"
                     /locus_tag="VD737_03100"
     CDS             complement(5738..6280)
                     /gene="def"
                     /locus_tag="VD737_03100"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXW09574.1"
                     /translation="MAKLTILEYPDPRLRTKAQPVTTVDDSIRQLVDDLFETMYEAKG
                     IGLAATQVDVHRRVLVTDVSADRTEPRVFINPQVIERDGSIEAEEGCLSVPGIFDTLR
                     TRSEKIRVRALDRDGEAFEIDADGLLAVCIQHEIDHLDGKLFVDYLSELKRSRIRKKL
                     EKERRERVPAAASPVRTPAL"
     gene            6460..7698
                     /locus_tag="VD737_03105"
     CDS             6460..7698
                     /locus_tag="VD737_03105"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HXW09575.1"
                     /translation="MDRYLRPESARVQTPRAWARALVLAAAVAGAGIGAGLAVPGAAL
                     AQADASRSMSVPIAASAPERYVVKRGDTLWDISAMYLQDPWYWPEIWYVNPNIANPHL
                     IYPGDVLYFSYVDGKPRVSLERAGTVRLSPEVRTSPLDGAIRAIPYDLLMDFVGRPRL
                     LDKDQIKDAPYVVGMRDRHIIGSDHNEIYGRGLENPAVGTRYNIISVGREMRDPDDGD
                     VLGYMGHFAGLGEIVQSTGAVIPGKDSIFKMKRDEELAHIKVLEVGREIVQGDKLFPA
                     TVDVGADFVITLPKNEGVLGQVIAVVDGVSVAGRYQVVAINRGKQHGLEPGNALGVFY
                     RGEEIRDRYDRIDWMRWTANYDKVQLPDERSATALVFQVYDRMSYALIVESSQALHVG
                     DFIAHPQYGHRDAGNVLYMR"
     gene            7769..8890
                     /gene="dprA"
                     /locus_tag="VD737_03110"
     CDS             7769..8890
                     /gene="dprA"
                     /locus_tag="VD737_03110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HXW09576.1"
                     /translation="MEGRDAWLTLAHAHGLHAGRLQEWFAQGRPAVELPGATSATLAA
                     LGLDADTVASLQAPNRAALDRDRAWLDAAPNRSLVVWGADDYPPRLAQIPDAPLVLYV
                     EGAIPALSTPQLAIVGSRNPTALGRDTATSFARELGRAGLGITSGLALGIDAAAHLGA
                     LEAGATTVAVVGRGLDAVYPRENEPLARRILAAGGALATDLPVGSPPLRHHFPRRNRI
                     LSGLAVGVLVVEAAEQSGSLITARLAAEQGRDVFAMPGSIHNAVARGCHRLLRQGARL
                     VETVGDIFEELAGICGDMHGLRAPSAPVVQSLQGPSLDKDYEILLDALGFEPASLDAI
                     VARTRLDTGAVASMLLILELDGRIQQQPGGRYSRRLPGR"
     gene            8892..9374
                     /locus_tag="VD737_03115"
     CDS             8892..9374
                     /locus_tag="VD737_03115"
                     /inference="COORDINATES: protein motif:HMM:NF016264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HXW09577.1"
                     /translation="MKEGVLDILIYLFENYFDADTDDGFEPDRETLKLELEKAGFPGS
                     EVERALCWLEELAADHPARLPPSPTSRAIRVFAALEQARLDTDCRGYLVHLEQVGILS
                     PMQRELVIDRLMALEGDDIDIDKLKWVVLMVLFSQPGQETAFSRMEDLVFEDRSGAIH
                     "
     gene            9516..>11973
                     /locus_tag="VD737_03120"
     CDS             9516..>11973
                     /locus_tag="VD737_03120"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HXW09578.1"
                     /translation="MTKNLVIVESPAKAKTIKKYLGKDFEVMASYGHVRDLVPKEGAV
                     DPERNFEMRWELIDKNRKHVDAIARAIRKADALYLSTDPDREGEAISWHLYQLLRESG
                     ALDGKSVHRSVFYEITRNAVQEAVDNPREIADELVKAYLARRALDYLVGFKVSPLLWK
                     KAGLSGASAGRVQSPALRMICEREEEILAFKPREYWTIDAQAQKDAAQFTAKLTQLAG
                     NKVEQFTVTNATDAAAAKRQLEQAAAGTLVVTNVEKKQRKRNPAAPFTTSTLQQEAAR
                     KLGYGAQRTMRLAQRLYEGVDFGEGPVGLITYMRTDSVSLAAEAVREIRDVIGRLYGR
                     EALPEAPRLYRTKSKNAQEAHEAVRPTSAARTPEMLAGRIDADEHKLYTLIWRRAVAS
                     QMEAAVFDTVAVDLAAGRDNTFRANGSTLLSPGFLAVYRESFDDVTLNADDDENRILP
                     PLEAGDRVTLLDIDTAQHFTEPPPRYSEASLVKALEEHGIGRPSTYASIIQTLLYKKY
                     CELVNRRFVPTDLGKIVNRFLTANFEHWVDYGFTASMEDELDNISRGEEDWLPMLQRF
                     WTELKKQIDDVDQNVTRSDVAMERPLGIDPVSGRPVSVRYGRFGAFAQIGTREDEEKP
                     KFASLRPHQRMDEIGLEDALELFQLPRTLGTWDDGHAIKVAVGRFGPYVQYGAKKYAS
                     LKKEDDPYTITLERARELINEKIQVEANRIIREFDDGSGIQVLNGRYGPYISDGKKNG
                     KIPKDRDPKSLTLEECKALLAAAPERKGWGRRATAKTAPARTTNGDGATAVASGTAVP
                     DGRKAAAPRKSTAAKQKTATR"
BASE COUNT         1895 a         4168 c         4133 g         1777 t
ORIGIN      
        1 atcgtgtgga tgtagcgcgc gaacgcctcg cgcccggtgc cgggctcgcc gagcacgagc
       61 acgggcgcct cgtgcggcgc gatctgcttc acctggtcgc gcaggtcacg catcaggcgg
      121 ctgcgcccga cgggggcgat cagcggcggc accagcgtcc gcgtcgctcg ttcgcggcgc
      181 cggccctcgt cgagcgcgcg ctgcaccgtg cgcaacagct tggcgagcga caacggcttc
      241 tcgacgtagt cgaccgcgcc caggcgggtc gcctcgaccg cggtctcgac cgtgccgtgg
      301 ccggacatca tcacgaccgg catgttgagg ccctgggact gctgccattc gcgcagcagg
      361 ctgatgccgt cggtgtccgg catccagatg tcgagcagca cgagctcggg cgcctggcga
      421 gcgcacgccg cacgcgcttc ggcggcggtg ccggccgtct cgaccgtgta cccctcctcg
      481 gtgaggatct cccggacgag ctcgcgaatg tcgttctcgt cgtcgacgac gaggatgcgc
      541 gcgtttgtca tgcgcgttct ctccttggct ccgatgtacc gcgcccggaa gcgccgctgc
      601 tgttgccgtt gcctctgcca cgctcggacc cggcgtcgtg caacggcagg tcgacgcaca
      661 cgcgcgcgcc gccggatggg cggttgtcgg cctcgatgtg gccgccgtgc tcttcgacga
      721 tcttgcgcac gatcgcgagg ccgagccccg tgcccttgac cttggtcgtg acgtagggat
      781 cgaacacctg gcctatcatt tcgcgctgga agccgggccc gtcgtcctcg accgcgatct
      841 tcgcgacctc ggtctcgccg tgcagcgcgc gctgcgtggt gaccgagatc gaaccctggc
      901 cctggccctc gagcgcttcg acggcgttgg tgagcaggtt gttgagcagc tggcgcagcc
      961 gtcctgcgtc cgccaccacg gagtcgatcg ccgggtcgag ctgcaccttg agccggatgc
     1021 tgcgcatcgc cccctgcgcg cgatagaggt cactcacctc ggtcaccatg cggttcaggt
     1081 cgaccgtcga catctcgagt cgcggcgcgc gcgcgtactc gctgaacgcg ttgaccatct
     1141 ccttcatcgc ctcgacctgc tgcacgatcg tctcggtcgc gcgctcgagc atctgcgcgt
     1201 ccttcgggtc gctcatcgcg ggcagcagcc ggtgacgcat gcgctcggcc gacagccgga
     1261 tcggcgtgag cgggttcttg atctcgtgcg ccaggcggcg cgcgacctcg ccccacgccg
     1321 cttcgcgctg ggcctgaagc atccccgtga tgtcgtcgaa caccagcacg taaccgcccg
     1381 gcgcgtcgcc ttcgcccggc agcggcgtgc aggcgcacat caggatgcgg cggctcgtcc
     1441 ctgccacgaa cgtgaactgc tcgcgccact cggtcgcgcc agcctcgcag cgctgccggc
     1501 atgcctcggc gaactgtcgc gccagtccct gcggctcggc ccccatcgcg tcgaaaccgg
     1561 cgccgacctg cgtctcgatg tcctcgccca ggatcatgct ggcggcgcgg ttggccgtgc
     1621 ggatgcgcca gtcgcgctcg agcgagatca cgccggtgga gaggcgcgcg aggatcaccg
     1681 cgaggtttgc gcgctcggtc tcgacggcct gttgcgcgcg cgacgattcc tcgcgggcgc
     1741 gacgcaggcg cttggtcatg tcgttgaacg agtggaccag gaagcccatc tcgtcgtgcg
     1801 acgtgagtgg cagcttggtg tcgaggtcgc cgtgcgcgac ggcacgcgtg ccctgcacga
     1861 ggtcctggat cggctgcacg aggcgctgcg ccgtgaagat cgcgccgtac cacgcggcaa
     1921 gcagcgacag cagcagcacg acggtcagcg tcaggatgaa gctcgccttg agcggctggc
     1981 gcaggtagtc cttctcgccg aactgcttgt acgccgcctg cacggtgtcg gcgagcgccc
     2041 ccagtcgctc ggccactgga tagaggccct gcacgaccag ccgctgctcg ccgggcaggc
     2101 tcacgccgat cgggacggcc gtgcggatca ggtagccgcc gtcctgctgc ggttcgaggc
     2161 tcacgaacgg ctggccctgc cgcacctgca gcagcacttc gtcgggcacg gcttcgggga
     2221 cgaggtcgtc ggagaagccc ttgctgacgg cgatgatcga gcccgagtca ttggcgacga
     2281 cgagctcgag cgcgccgacc tggcggcgca acgtttcgag caggcccgcc atccgcgggc
     2341 cgcgcatcac gctcagctcg tcggcgacca tcgtcgagtc ggcgaggtgc tcgcgcatgc
     2401 gcacctcgag cgccgagcgg gagagcacga gggcgtcgtc gaggctgcgg cggatctcga
     2461 cgttgaacca gctgtcgatg cccttgctca ggaactgcac cgcgaagaaa tagacgagca
     2521 ggatcggcag ggtcgcgagc acgccgaaga tccacaccat gcgcgccttg agccgcgagc
     2581 cgatcacgtg cccgcgccag tcgcggatca gctgcgcgag cttggtcgca agcagcccga
     2641 ccagcaccgc gaggcccgcg atgttgatca tcaggatcca cggatgcagg cggctgaact
     2701 gctccgagtt ctgcgcggtc tgcgccatga acaggatcgc ggcgagccac agcaggccac
     2761 ccacgcccac cgtgatggcc gatgcggtcc gcctcagggt cggagcgtcc atgtcttcca
     2821 gtcgctctcg gcgctccagt cgtccgccca gaacagcagc catccgaacg cgtccggcgc
     2881 ggtgctcaag tccagcacgg cacggatgcg tccctggtat tccggcccgg ccggcagcag
     2941 cgcggtgtcg atgatcggca gcccgcgcaa ttcggagatg cgcgcgagcg cggtgtcgag
     3001 cgagccgaag tcgtcctgct cgccggtgtt gaggttgcgc acgagccacc gctggctcag
     3061 tgcgtgataa gtgacctcgt agcgctgcac cagcgccgcg acctcggcgt cgagcatgta
     3121 gcgacgcact cggtcgatca cgatctcgta gttcaggcgc aacgtgaggc cggactcgac
     3181 ggcccggcgg gcgtcgtccg gcaggcgcag gtccaggcgc gcgtcgagtt cgtaaacgcc
     3241 gtcgtcgacg ccgatccgga cgtcagacag cgtgagcgac gcttgcgccg aggcgcgctc
     3301 cggggcggcc agcgttgcga ttacgcacag cagcagcggc agggtccatc gcgccaagaa
     3361 cggcgatctg ggcgcggcgg cggacgggct gtagcgcatt gccaacaatc ataacgactt
     3421 ggccagacaa gcataataaa agccgtccat gcccgattcg cccggcagga cctggtaccc
     3481 gggaccgtgc ccgtccggcc gcgccggcca cgcggccgtg agggcgggcg tcaggtcgcg
     3541 ggcgccacgg gtccgcgcga ggaagcccgc gaccacgcgc tcgttctcgg ccgccaggac
     3601 ggaacagctc gtgtacagca gccgcccgcc cggtcgcacg acctgccacg ccgcctcgag
     3661 caactcggcc tggcggcggg ccagcgcggg gatgtcgcgc gcgcggcgca ggatcttgat
     3721 gtccgggtga cggcggatca cgccggtcgc cgagcacggc acgtccagca ggacgcgatc
     3781 gaacggccgg ctgtcccacc actctgcgac cgcggcgacg tccgcgacgc ggatcgaggc
     3841 ctccaggccg agccggacca ggttttcgcg cacgcgctcg atacgcgtcg ccgagacgtc
     3901 gagggcggtc aggtcgacgg cgccgtccgt gcgttcgagc acgtggcagg tcttgccgcc
     3961 cggcgcggcg caggcgtcga ggatgcgctc gcccggcgcg ggctcgagca ggtcgatcgc
     4021 gagctgggcg gcggcgtcct gcaccgacag ccgaccctcg gcaaagcccg gcagcgaccg
     4081 cacgtctgtc ggcggctcga cctgtagcgc atcgctggcc cacgggtgac gcgtcgtcgt
     4141 gaagccagcc gtattcaaca cattagcggc ttcttcgatg ctcgttcgcc gacgattgat
     4201 gcgaacccaa agcggcgggt gcgcgttgtt ggcttcgagc acggccgggg ccgcatcgcc
     4261 gcggtcctgc cgcagtgcgt cgacgaacca ccgcgggtgc gcggtgcgga tcgacaggtc
     4321 gcggtccacc gcgtgcagca gtgcggcctg ttcgcgctgg aagcggcgca gcacggcgtt
     4381 cacgaacccg gcggccttgt cgaagccgag cgcacgggcg gcgctgaccg tttcggagac
     4441 ggcggcgtgt ggcgccgtct cgcccgacat cagctggtag aggccgacct cgagcagcgc
     4501 gcgaagccgc ggatcgagct gttcgggcgg tcgcgccgcg agcgcgttca gcactgccgc
     4561 caggcggaag tgccagcgca gcgtcccgta gcagagcgag cgcttgagcc cgcgcgccga
     4621 gccttcgtcc tgctcggccg ccagcagatc gtcgagcgag cggcccttcg ccagcacctc
     4681 ggcgacgatg cgcgcggcct cggccctcag ccgcgccggt gcggccgtgt tcacgcggcc
     4741 acgaccgtcg atgcgaagcg ccgtcccctc aggtcccgcg cgttcagcac gtcggcggcg
     4801 ctcacgggct tccgacccgg caactgcagt cgcagcagct cgagcgcgcc gtcgccggtg
     4861 gcgacgacga ggcgaccggg gtcgtcgagc accgtccccg gggacatctc tacctttccg
     4921 tcgggaaatc cggagtgtcc ctgatttccg gcggataaat aggagtgtcc ccattttccg
     4981 gccggccgtg cctcccagat gcgcacctgc tcgccgtcga ggcgcgtctc ggccacgggc
     5041 cagggattga aggcgcgcac gagccggtcg atctcgagcg tgggtcgcga ccagtcgatg
     5101 cgggcctcgt ccttgcggat cttggccgcg tacgtcgcgc ctgcctccgg ctgcgctcgc
     5161 gcgatcagcg tgccgctcgc gaccccggcc agcgcctcga caatggcggc cgcgcccagg
     5221 ggcgccagcc ggtcgtgcaa ctcaccgccg gtttcgcgcg acccgatcgc gagctcgcgc
     5281 cgcagcagct cgggccccgt gtcgaggccg gcgtccatct gcatgatcga gatgcccgtc
     5341 tgcgcgtcgc cggccagcag tgcgcgctgg atcggtgcgg cgccgcgcca gcgcggcagc
     5401 agcgacgcgt ggatgttgat gcagccgagg cgcggtatgt cgagcacggg ctgcggcagg
     5461 atcaggccgt acgcgacgac gaccatgacc tcaggcgcgt gcgacgcgag gcgcgcctgt
     5521 gcctcgggcg tccgcaacgt cgccggttgc tcgacgacca gccccagctg ctccgcgcgt
     5581 cgcttgaccg ggctcgacgc gagcgcgcga ccgcgaccgg caggacggtc gggctgcgtg
     5641 tagaccgcga cgatcgaatg gccggccgcc tgcaatgcgt cgagcgccgg caccgaaaac
     5701 tcgggcgtgc cggcaaacac gatgcgcagc gccggcgtca caaagccgga gtgcgcacgg
     5761 ggctggcggc cgccggtacg cgctcgcggc gctccttctc gagcttcttg cggatgcggc
     5821 tgcgcttgag ctcggacagg taatcgacga acagcttgcc gtcgaggtga tcgatctcgt
     5881 gctggatgca gaccgccagc agcccgtccg cgtcgatctc gaaagcctcg ccgtcgcggt
     5941 ccagtgcgcg cacgcggatc ttctccgacc gtgtccgcag ggtgtcgaag atgccgggga
     6001 cggaaagaca gccttcctcg gcctcgatcg agccgtcgcg ctcgatgacc tgcgggttga
     6061 tgaacacccg cggctccgtg cggtccgccg agacgtccgt cacgagcacg cgccggtgca
     6121 cgtctacctg cgtcgccgcg aggccgatgc ctttcgcctc gtacatcgtc tcgaacaggt
     6181 cgtcgacgag ctgccggatc gagtcgtcca cggtcgtcac cggctgtgcc ttggtgcgca
     6241 accgcgggtc cggatactcg aggatcgtga gcttcgccat tcgtttgaaa atactgctaa
     6301 agttaagcca cgcttgaagt tactgctcgc aagaacgagc gtccgggccc cggaaaacgg
     6361 ctcgtcgcgt ggcgcgactg agacgtggtc ggcagccgcc gccgggtggc ccggattata
     6421 agacggcggt cacgaccccg atacccgatg gagaaaggga tggatcgtta tcttcgacct
     6481 gagtcggccc gagtgcagac gccgcgggcg tgggcgcggg ccctggtgct cgccgccgcc
     6541 gtcgcgggcg cgggtatcgg cgccggcctc gccgtcccgg gcgcggcact cgcccaggcc
     6601 gatgcgagcc gctcgatgtc ggtcccgatc gcggccagcg cgccggaacg gtacgtggtc
     6661 aagcggggcg acacgctgtg ggacatctcg gcgatgtacc tgcaggaccc gtggtactgg
     6721 cccgagatct ggtacgtcaa cccgaacatc gcgaacccgc acctgatcta tcccggtgac
     6781 gtgctgtact tcagctacgt cgacggcaag ccgcgcgtct cgctcgagcg ggccggcacc
     6841 gtgcgcctgt cgcccgaagt gcgcacctcg ccgctcgacg gcgccatccg cgcgattccc
     6901 tacgacctgc tgatggactt cgtcggccgc ccgcggctgc tcgacaagga ccagatcaag
     6961 gatgccccgt acgtcgtggg catgcgcgac cgtcacatca tcggcagcga tcacaacgag
     7021 atctatggcc gtggcctcga gaaccccgcg gtgggcacgc gctacaacat catcagcgtc
     7081 ggccgggaaa tgcgcgatcc ggacgacggc gacgtgctcg gctacatggg ccatttcgcc
     7141 gggctcggcg aaatcgtcca gagcaccggt gccgtgatcc ccggcaagga ttcgatcttc
     7201 aagatgaagc gcgacgagga actcgcccac atcaaggtgc tcgaggtggg ccgcgagatc
     7261 gtgcagggcg acaagctgtt ccccgcgacg gtcgacgtgg gcgccgactt cgtgatcacg
     7321 ctgccgaaga acgagggcgt gctcggccag gtcatcgccg tcgtggacgg cgtgtcggtc
     7381 gcgggccgct accaggtggt cgcgatcaac cggggcaagc agcacgggct cgagcccggc
     7441 aacgcgctcg gcgtgttcta ccgcggcgag gaaatccgcg accgctacga ccgcatcgac
     7501 tggatgcgct ggaccgcaaa ctacgacaag gtgcagctgc cggacgagcg cagcgccacg
     7561 gcgctcgtgt tccaggtcta cgaccgcatg agctacgcgc tgatcgtcga gtcgtcgcag
     7621 gcgctgcacg tcggcgactt catcgcccac ccgcagtacg gccatcgcga cgccggcaac
     7681 gtgctctaca tgcgctgacc atttttccgc tgacgtcgca gatatcgcga aagcctgccg
     7741 gggcgttgct ccggcaggct ttttcgtcat ggaaggccgc gacgcctggc tcacgctcgc
     7801 gcacgcgcac ggcctgcacg ccggccggct gcaggagtgg ttcgcgcagg ggcgcccggc
     7861 cgtcgagctg ccgggcgcca cgtccgcgac gctcgccgcg ctcggcctcg acgccgacac
     7921 cgtcgcctcg ctgcaggcgc cgaaccgggc ggccctcgat cgcgaccggg cctggctcga
     7981 cgcggccccg aaccggtcgc tcgtcgtctg gggcgcggat gactaccctc cgcgactcgc
     8041 gcagatcccg gacgcgccgc tggtgctgta cgtcgagggc gcgattcccg cgctctcgac
     8101 gccgcagttg gcgatcgtgg gcagtcgcaa cccgaccgcg ctgggccgcg atacggcgac
     8161 gtcgttcgcg cgcgagctcg gtcgcgccgg cctcggcatc acgagcggcc tggcgctggg
     8221 aatcgacgcg gccgcgcacc tcggcgcgct cgaggccggc gccacgaccg tggccgtggt
     8281 cggtcgcggc ctcgacgcgg tgtatccgcg tgagaacgaa cccctggccc ggcgcatcct
     8341 cgccgcggga ggcgcgctcg ccaccgacct gccggtgggt tcgccgccgc tcaggcatca
     8401 ctttccgcgc cgcaaccgga tcctgagcgg cctggcggtc ggcgtcctgg tcgtggaggc
     8461 ggccgaacag agcggatcgc tgatcacggc ccgcctggcg gcggagcagg gccgcgacgt
     8521 gtttgccatg ccgggatcca tccacaacgc cgtggcccgc ggctgccatc ggctgctgcg
     8581 gcagggcgcc cggctggtgg agacggtggg cgacatcttc gaggaacttg ctggcatctg
     8641 cggcgatatg catggcttaa gggcgccatc ggcgccggtc gtgcaatcac tgcagggacc
     8701 ctcgttggac aaggactatg aaatcctgtt agatgcgctc ggcttcgaac cggccagcct
     8761 cgacgcgatc gtcgcgcgta cgcgactgga taccggcgcc gtcgcctcaa tgctgctcat
     8821 cctcgagctg gacgggcgga tacaacagca accgggcggg cgatacagcc gccggttgcc
     8881 gggaaggtga aatgaaggaa ggcgtcctcg acatcctcat ctatctgttc gagaactact
     8941 tcgacgccga cacggacgat ggcttcgaac ccgatcgcga aacgctgaag ctcgagctgg
     9001 agaaagccgg tttccccggc agcgaggtcg agcgcgcgct gtgctggctc gaggaactgg
     9061 cggccgacca cccggcccgc ctgccaccgt cgccgacctc ccgtgccatt cgggtcttcg
     9121 cggcgctgga acaggcgcgc ctcgacacgg actgccgcgg ctacctggtg cacctcgagc
     9181 aggtcgggat cctgtcgccg atgcagcgcg agctcgtcat cgaccggctg atggcgctcg
     9241 aaggcgacga catcgacatc gacaagctca agtgggtggt gctgatggtg ctcttcagcc
     9301 agcccgggca ggaaaccgcg ttttcgcgga tggaagacct ggtgttcgag gatcgcagcg
     9361 gagccatcca ctagcgggta ggggttagcg gttagcggtt agtcgaacgc tcttcgtcgc
     9421 gccgaaggcg ctcgtgctaa ccgctgatca ctaaccgcta accgctcgca ttagcgcctg
     9481 ccgcggaccg ccgcggcatg ttcgtttctg aggacatgac caagaatctc gtcatcgtcg
     9541 agtcgccggc caaggccaag accatcaaga agtacctggg caaggacttc gaggtgatgg
     9601 cgtcctatgg ccacgtccgc gacctcgttc ccaaggaggg cgccgtcgat cccgagcgca
     9661 atttcgagat gcgctgggag ctgatcgaca agaaccgcaa gcacgtcgac gcgatcgctc
     9721 gcgcgatccg caaggcggac gcgttgtatc tctcgaccga ccccgaccgc gagggcgagg
     9781 cgatttcgtg gcacctgtac cagttgctgc gggagtccgg cgcgctcgac ggcaagagcg
     9841 tgcaccgcag cgtcttctac gagatcacca gaaacgcggt gcaggaggcg gtcgacaacc
     9901 cgcgcgagat cgccgacgaa ctcgtcaagg cctacctcgc tcgccgcgcg ctcgactacc
     9961 tggtcggctt caaggtgtcg ccactgctgt ggaagaaggc cggactttcg ggcgcgtccg
    10021 ccggacgcgt gcagagcccc gcgctgcgga tgatctgcga gcgcgaggag gagatccttg
    10081 cgttcaagcc gcgcgaatac tggacgatcg acgcgcaggc gcagaaggac gcggcgcagt
    10141 tcaccgccaa gctcacgcag ctggccggca acaaggtcga gcagttcacg gtcaccaacg
    10201 cgaccgacgc ggccgcggcg aagcgccagc tcgagcaggc cgccgccggc acgctcgtcg
    10261 tcacgaacgt cgagaagaag cagcgcaagc gcaacccggc agcgccgttc acgacgtcga
    10321 cgctgcagca ggaggccgcg cgcaagctgg gctacggcgc gcaacgcacg atgcgcctcg
    10381 cccagcgcct gtacgagggc gtggacttcg gcgaaggccc ggtcggcctg atcacgtaca
    10441 tgcgcaccga ctcggtcagc ctcgccgcgg aggccgtgcg cgagatccgc gacgtgatcg
    10501 gccggttgta cggacgggag gcgctccccg aggcaccgcg cctgtatcgc accaaatcga
    10561 agaacgcgca ggaggcccac gaggcggtgc ggccgacctc cgctgcgcgt acgcccgaga
    10621 tgctcgcggg caggatcgac gccgacgagc acaagctcta cacgctgatc tggcgccgcg
    10681 ccgtcgcgag ccagatggaa gcggcggtgt tcgatacggt cgcggtcgac ctggccgcgg
    10741 gccgcgacaa cacgttccgc gccaatggct cgacgctgct gtcgccaggc ttcctcgccg
    10801 tgtatcgcga gagcttcgac gacgtcacgc tcaacgccga cgacgacgag aaccgcatcc
    10861 ttccgccgct cgaggccggc gaccgcgtga cgttgctgga catcgacacc gcgcagcatt
    10921 tcaccgagcc gccgccgcgt tactccgagg ccagcctggt caaggcactc gaggagcacg
    10981 gcatcggccg cccgtcgacg tacgcgagca tcatccagac gctgctctac aagaagtact
    11041 gcgagctcgt gaaccggcgc ttcgtgccga ccgacctcgg caagatcgtc aaccgcttcc
    11101 tgaccgcgaa cttcgagcac tgggtcgact acggcttcac ggcgtcgatg gaggacgagc
    11161 tcgacaacat ctcgcgcggc gaggaggact ggctgccgat gctccagcgc ttctggaccg
    11221 agctcaagaa gcagatcgac gacgtcgacc agaacgtcac gcgctccgac gtcgcgatgg
    11281 agcgcccgct cggcatcgat ccggtgtcgg gccgcccggt gagcgtccgc tacggacgct
    11341 tcggcgcgtt cgcgcagatc ggcacgcgcg aagacgagga aaagccgaag ttcgcgagcc
    11401 tgcgtccgca ccagcgcatg gacgagatcg ggctcgagga tgcgctcgag ctgtttcagc
    11461 tgccgcgcac gctcggcacg tgggacgacg gtcacgccat caaggtcgcg gtggggcgct
    11521 tcggacccta cgtgcagtac ggcgcgaaga agtacgcgtc actcaagaaa gaggacgacc
    11581 cgtacacgat cacgctcgag cgcgcgcgcg aactgatcaa cgaaaagatc caggtcgagg
    11641 ccaaccggat cattcgcgag ttcgacgacg gctccggcat ccaggtgctg aacggccgct
    11701 acggcccgta catcagcgac ggcaagaaga acggcaagat cccgaaggac cgcgacccga
    11761 agtcgctgac gctcgaagag tgcaaggcgc tgctcgccgc ggcgccggag cgcaagggct
    11821 ggggccgtcg ggcgacagcg aagacggcgc cggccaggac gaccaacggc gacggtgcca
    11881 ccgcagtcgc ctcgggcacc gccgtgccgg acggccgcaa ggccgcggcg ccgcgcaagt
    11941 cgaccgccgc caagcagaag accgcgaccc gca
//
LOCUS       DASPFR010000123         2887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_158553, whole genome shotgun sequence.
ACCESSION   DASPFR010000123 DASPFR010000000
VERSION     DASPFR010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158553"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="VD737_03125"
     CDS             <1..941
                     /locus_tag="VD737_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HXW09579.1"
                     /translation="PERAHAQNAIKIDGSSTVFPISEAYAEEFQIQKRGKVRVTVGVS
                     GTGGGFKKFCRGETDMANASRPISVEEMEACRKAGIKYMEVPVAFDALTVVVNPANTW
                     AKSLTVAELKKMWEPAAQGRITTWNQINPKFPKEKIMLFGPGADSGTFDYFTEAVMGK
                     SKASRGDYTASEDDNTLVQGVENNKNAIGYFGFAYYAAHKDKMTAVGIDPGTGKAVLP
                     SLENVTNGSYTPLSRPLFVYVRDSSAKRPEVREFVQFMLQNGDLVKEVGYLPLPKQAY
                     DLAWQHFGSGKLGTVFGGQAKVGITIDQLLAMEAKL"
     gene            1022..1966
                     /gene="pstC"
                     /locus_tag="VD737_03130"
     CDS             1022..1966
                     /gene="pstC"
                     /locus_tag="VD737_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HXW09580.1"
                     /translation="MSSVTLPPRTGVALGKGAFASLRYRKLRDRVVEFVLLACGLVAV
                     FTTLAIVAILIFESVSFFEHVSFRDFVTDTMWTPLFADARYGIMPLVSGTLTVTMVAL
                     LVAIPLGTIIAIYLSEFASHRIRESVKPVLELLGAVPTVVYGYFALLTVTPFLQKLIP
                     GLPGFNMLSAGLVIGLMIVPYVASVSEDAMRAVPRYMREGSYAMGATKLQTALRVVVP
                     GAFSGIAAAYILGISRAIGETMVVAIAAGMQPTLTFNPTEPAATITAYIVQVSLGDLP
                     HGSIGYQSIFAAGLVLMLMTLVFNVLGFFLTRKFREAY"
     gene            1968..>2887
                     /gene="pstA"
                     /locus_tag="VD737_03135"
     CDS             1968..>2887
                     /gene="pstA"
                     /locus_tag="VD737_03135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HXW09581.1"
                     /translation="MAAVMPLEQLRADLAWRKNLDKLFVVLGLVILLVCLGLLALLFF
                     DLVRDGASRFGWDFLTQFPSRKAERAGILSAGVGTIMIMFVTAVVALPVGVAAAIYLE
                     EYARKNWFSGIIEINVTNLAGVPSIVYGLLALGLFVYTFGLGQSVLSAGLTLALLILP
                     IVIVATREALRAVPKAIREAAYGLGATRWEVTKDHVLPYSTGGILTGLILGLSRAIGE
                     TAPIITIGALSFIAFLPPSPVTSQFPFVDFEWLRSEFTAMPIQMFNWISRPDQAFQAN
                     AAAAGAILLGMTLLMNAVAIWIRYRFRKKI"
BASE COUNT          458 a          938 c          971 g          520 t
ORIGIN      
        1 gccctgagcg ggcgcacgcc cagaatgcga tcaagatcga cggctcgagc acggtgttcc
       61 cgatctccga ggcgtacgcc gaggaattcc agatccagaa gcgcggcaag gtccgcgtga
      121 cggtcggcgt gtcgggcacc ggcggcgggt tcaagaagtt ctgccgcggc gagaccgata
      181 tggccaacgc gtcgcgcccg atctcggtcg aggagatgga agcctgccgc aaggccggca
      241 tcaagtacat ggaagtgccg gtcgcgttcg acgcgctgac ggtcgtggtc aatccggcca
      301 acacctgggc caagtcgctg accgtcgccg agctcaagaa gatgtgggag ccggccgcgc
      361 agggccgcat cacgacgtgg aaccagatca acccgaagtt ccccaaggaa aaaatcatgc
      421 tgttcgggcc gggcgccgac tcgggcacgt tcgactactt caccgaggcg gtgatgggca
      481 agtccaaggc gagccgcggc gactacaccg cgagcgagga cgacaacacg ctggtgcagg
      541 gcgtcgagaa caacaagaac gcgatcggct acttcggctt cgcctattac gccgcgcaca
      601 aggacaagat gaccgcggtg ggcatcgacc cgggcacggg aaaggccgtg ctgccgagcc
      661 tcgagaacgt caccaacggc agctacacgc cgctgtccag gcccctgttc gtgtacgtgc
      721 gtgacagctc ggcgaagcgt cccgaggtgc gggaattcgt gcagttcatg ctgcagaacg
      781 gtgacctcgt gaaggaagtc ggttacctgc cgctgccgaa gcaggcgtac gacctcgcgt
      841 ggcaacattt cggcagtggc aagctcggca ccgtgttcgg cgggcaggcc aaggtgggca
      901 tcaccatcga ccagctgctc gcaatggaag ccaagctgta agcttccaaa ttcgtggacg
      961 ggccggggac ctccccgtgc ccgtccgcgc accggcggtt gccgcgagag gtctacgcca
     1021 gatgtcgtcc gtcacgttgc caccccgcac gggggttgcg ctcggcaagg gcgcattcgc
     1081 gtcgctgcgc taccgcaagc tgcgcgaccg cgtggtcgaa ttcgtgctgc tcgcctgcgg
     1141 gctggtggcc gtgttcacga cactggcgat cgtcgcgatc ctgatcttcg agtcggtctc
     1201 gttcttcgag cacgtctcgt tccgcgactt cgtcaccgac acgatgtgga cgccgctctt
     1261 cgccgacgcg cgctacggca tcatgccgct cgtctccgga acgctcacgg tgacgatggt
     1321 ggcgctgctg gtggcgatcc cgctcggcac gatcatcgcg atctacctgt cggagttcgc
     1381 gtcgcaccgg atccgcgagt ccgtcaagcc ggtgctcgag ctgctcggcg ccgtgccgac
     1441 ggtcgtctac ggctatttcg cgttgctgac ggtgacgccg ttcctgcaga agctgatccc
     1501 ggggttgccg gggttcaaca tgttgagcgc cggactcgtg atcggcctga tgatcgtgcc
     1561 ttacgtggcc tccgtcagtg aggacgcgat gcgggcggtg ccgcgctaca tgcgcgaggg
     1621 ttcgtacgcg atgggcgcga ccaagctgca gacggcgttg cgcgtggtcg tgccgggtgc
     1681 gttctcgggc atcgccgcgg cgtacatcct gggcatttcg cgggcgatcg gcgaaacgat
     1741 ggtcgtcgcg atcgcggccg ggatgcagcc gacgctgacg ttcaacccga ccgagccggc
     1801 cgccacgatc accgcctaca tcgtgcaggt gagcctcggt gacctgccgc acggcagcat
     1861 cggctaccag agcatcttcg cggcggggct ggtgctgatg ctgatgacgc tggtcttcaa
     1921 cgtgctcggt ttcttcctga cgcgcaagtt ccgcgaggcc tactgacgtg gccgccgtga
     1981 tgccgctcga gcagctgcgc gccgacctcg cctggcgcaa gaacctggac aagctcttcg
     2041 tcgtgctggg gctcgtgatc ctgttggtct gcctcgggtt gctggcgctg ctgttcttcg
     2101 acctggtgcg cgacggggcc tcacgcttcg gctgggattt cctgacgcag tttccgtccc
     2161 gcaaggccga gcgcgccggc atcctgtccg ccggcgtcgg cacgatcatg atcatgttcg
     2221 tgaccgccgt ggtcgcgctg ccggtcggcg tcgccgcggc gatctacctc gaggagtacg
     2281 cgcgcaagaa ctggttcagc gggatcatcg agatcaacgt gaccaacctc gcgggcgtcc
     2341 cgtcgatcgt gtacggcctg ctcgcgctcg ggctgttcgt ctacacgttc ggcctgggcc
     2401 agagcgtgct gtccgccggt ctcacgctgg cgctgctgat cctgccgatc gtgatcgtcg
     2461 cgacgcgcga ggcgctgcgt gcggtgccga aggcgatccg cgaagcggcc tacgggctcg
     2521 gcgcgacgcg ctgggaagtc accaaggacc acgtgctgcc gtactcgacc ggcggcatcc
     2581 tgacggggct gatcctgggc ctttcgcggg cgatcggcga gaccgccccg atcatcacga
     2641 tcggcgcgct gagcttcatc gcgttcctgc cgccgtcacc ggtaacctcc cagttcccgt
     2701 tcgtcgattt cgagtggctc cgcagcgagt tcacggccat gccgatccag atgttcaact
     2761 ggatctcgcg gcccgaccag gccttccagg ccaatgcggc ggccgccggt gcgatcctgc
     2821 tcgggatgac gttgctgatg aacgcggtgg cgatctggat ccgctaccgt ttccgcaaga
     2881 agatcaa
//
LOCUS       DASPFR010000124         6036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1624081, whole genome shotgun sequence.
ACCESSION   DASPFR010000124 DASPFR010000000
VERSION     DASPFR010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6036
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624081"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1072)
                     /locus_tag="VD737_03140"
     CDS             complement(65..1072)
                     /locus_tag="VD737_03140"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HXW09582.1"
                     /translation="MPVELTIPMATLVLLVALALAFDFMNGFHDAANSIATVVSTGVL
                     RPHYAVAWAAFFNFVAIFIFHLKVAATVGKGIVDPVAIDHYVIFGALIGAIAWNLITW
                     YYGIPSSSSHALIGGMIGATISKAGTDPLVAAGIIKTATFIFVSPLLGMALGALLMIA
                     VSWICLRSAPRRVDRWFRRLQLASSAAYSIGHGSNDAQKTMGIIWLLLISAGVTTPDH
                     LPSWVIVSCYVAIAFGTMFGGWRIVKTMGQKITKLRPVGGFAAETGGAITLFLASSLG
                     IPVSTTHTITGAIIGVGSAHRTRAVRWGIAGNIVFAWIVTMPAAALMAALAYYLGRLL
                     I"
     gene            complement(1072..1713)
                     /locus_tag="VD737_03145"
     CDS             complement(1072..1713)
                     /locus_tag="VD737_03145"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HXW09583.1"
                     /translation="MRFLSALMPRETRFFDLFDRHGGLIVEGGDAIVELVRHYEDTAR
                     RAQLIQRIGDVERSADKITHETISVLHSTFITPFDRNDIHRLISGMDDVLDLMQDAAE
                     SMHLFDIQTLPPASLKMAELLQSCCTRVRTGVGLLRSMDNAARALELTEEIDQLESEA
                     DAVSRDGISRLFREEQDVRQLIKHKNVLEYLEDAIDKCQDVANVIESVVVENV"
     gene            1820..>6036
                     /gene="uvrA"
                     /locus_tag="VD737_03150"
     CDS             1820..>6036
                     /gene="uvrA"
                     /locus_tag="VD737_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXW09584.1"
                     /translation="MSDSSHDCIRVRGARQNNLRGLDLDIPLRQLVVVTGVSGSGKSS
                     LVFDTVYAEGQRRYVETFSPYARQFLDRMDKPQVDRIDGIPPAIAIDQTNPVRTSRST
                     VGTMTELNDHLKLLFARAAQLHCRGCGGPVRRDTPDSIYAALTDRAAPLGDPRLVVTF
                     PVTVPPSFTEAEVVKLLEQQGYTRIHSRQPAHVEVVQDRFRLGGAERARVIDALESAL
                     RVGRGRVSVRTSPVSPSKEAEVARSAGEGPADRQPSPAGAGEGEAEEVWRFSTDLHCA
                     DCDIHYREPAPSLFSFNSPLGACETCRGFGRVIGIDYGLVVPDVAKTLRGGAVKPWQT
                     ESNRECQDDLLRYAKKRDVPVDVPWRDLTPDQQRWVLEGEGPWEKKVWYGVRRFFQWL
                     ESKSYKMHVRVLLAKYRSYTPCPTCHGARLKDEALLWRLATSDRPDAALNIHQVMLLS
                     LADCLGFFERLALPAPLDEATAQLLDEVRARLGFLVRVGVGYLTLDRQSRTLSGGEVQ
                     RINLTTALGTSLVNTLFVLDEPSIGLHPRDMKRVIEVMHRLRDNGNSLLVVEHDPQIM
                     LTADRIVDMGPGPGEHGGRVVFEGTPAELAVRSDTLTAQYLSGRRDAGVSAGRRAPPR
                     PGDPVIRVCGATEHNLHDLDVEFPLHRLVCVTGVSGSGKSTLVQDVLYAGLRRAKGRP
                     TETPGAHREIVGAELIDDVVMVDQTPIGRTTRSNPASYVGAFEAIRKRFARAPLALER
                     GYTVGTFSFNSGNGRCPTCGGNGFEHVEMQFLSDVYLRCPDCDGKRFRPEVLEVRVER
                     HGRSLDVAQVLELTVSEAVQFFAEDADVLARLEPLAAVGLDYLRLGQPVPTLSGGEAQ
                     RLKLAGFLADVGERPEKSGQSPFSQSGKGGQSRTLFLFDEPTTGLHFEDVSKLLEAFK
                     RLLDAGHSLLVIEHNLDVIRASDWIVDLGPEGGDGGGRLMVAGTPQTVMAATNSHTGH
                     ALREYERERAEVVRAGQARLPSFTLPATKRDAVADRARDSIVVHRAREHNLRDIEVTI
                     PRECMTVLTGVSGSGKSTLAFDIVFNEGQRRYLESLNAYARQFVQPAARPDVDAIFGI
                     PPTVAIEQRTSRGGRKSTVGTLTEVHHFLRLLYVKLGTQFCPDCQVPIEPQSADSIAA
                     LLLREARGRRIELLAPLVVARKGVYTDLAKWAASKGYAELRVDGAMLPTAKWPRLDRF
                     REHTIELPVGALKVTPEHEAGLRATLDRALEFGKGVAHVVDRGKTRVFSTLRACPSCG
                     RSFAEPDPRMFSYNSKHGWCDGCYGTGLQISGFDAEQSGEEVWWNEWFEGEAAACTSC
                     DGRRLNPVALNVRFRGLSIADLSALPVEQALAFFEALVLTGREAEIARDLRAEAGSRL
                     VFLRDVGLGYLSLDRSAPTLSGGEAQR"
BASE COUNT          939 a         2019 c         2129 g          949 t
ORIGIN      
        1 gagcaggtgc ggatacggca tcgacgaacc ctcgcgggtt tcttctggaa gtggcagggg
       61 accgtcagat cagcaggcgt ccgaggtaat aggccagggc ggccatcagc gccgcggccg
      121 gcatcgtgac gatccaggcg aagacgatgt tgcctgcgat gccccagcgt accgcgcgcg
      181 tccggtgcgc cgagccgacg ccgatgatcg cgccggtgat cgtgtgcgtc gtcgacaccg
      241 gaatccccag gctcgacgcg aggaacagcg tgatcgcgcc cccggtctcg gccgcgaagc
      301 caccgaccgg acgcagcttg gtgatcttct ggcccatggt cttcacgatg cgccagccgc
      361 cgaacatggt gccgaacgcg atcgcgacgt agcagctgac gatgacccag ctgggcaggt
      421 ggtcgggcgt ggtcactccg gccgagatca gcagcagcca gatgatgccc atggtcttct
      481 gggcgtcgtt gctgccgtgc ccgatgctgt aggccgcgga ggaggcgagc tgcagtcgcc
      541 tgaaccagcg gtcgacgcgg cgcggcgcgg accgcaggca gatccaggag acggcgatca
      601 tcagcagcgc gccgagcgcc atgccgagca gcggcgagac gaagatgaac gtggccgtct
      661 tgatgatgcc cgccgcgacc agcgggtcgg tgcccgcctt ggagatcgtc gcgccgatca
      721 tcccgccgat cagcgcgtgc gacgagctcg acgggatgcc gtagtaccag gtgatcaggt
      781 tccaggcgat cgcgccgatc agcgcgccga agatcacgta gtgatcgatc gcgaccgggt
      841 cgacgatgcc cttgccgacg gtcgccgcca ccttcaggtg gaagatgaag atcgcgacga
      901 aattgaagaa agctgcccag gcgaccgcgt agtgcggccg cagcacgccc gtcgacacga
      961 ccgttgcgat cgagttggcc gcgtcgtgga agccgttcat gaaatcgaag gcgagcgcca
     1021 gcgcgacgag cagcacgagc gtcgccatcg ggatggtcag ctcgacgggc atcagacgtt
     1081 ttccacgacc accgattcga tgacgttggc cacgtcctgg cacttgtcga tcgcgtcctc
     1141 gaggtattcg aggacgttct tgtgcttgat cagctgccgc acgtcctgtt cctcgcggaa
     1201 cagccgcgag atcccgtcgc gggacacggc atcggcctcg ctctcgagct ggtcgatctc
     1261 ctcggtgagt tcgagcgcgc gcgccgcgtt gtccatcgag cgcagcagtc cgacgcccgt
     1321 gcggaccctg gtgcagcacg actgcagcag ctcggccatc ttcagcgagg cgggcggcag
     1381 cgtttggatg tcgaacaggt gcatcgactc agccgcgtcc tgcatcaggt cgagcacgtc
     1441 gtccatgccg gagatcaggc ggtggatgtc gttgcggtcg aacggcgtga tgaacgtcga
     1501 gtgcaggacc gagatggtct cgtgcgtgat cttgtcggca ctgcgctcga cgtcgccgat
     1561 gcgctggatc agctgggcgc ggcgggcggt gtcctcgtag tgccggacca gctcgacgat
     1621 cgcatcgccg ccctcgacga tcaggccgcc atggcggtcg aacaggtcga aaaaccgcgt
     1681 ctcgcgcggc atcagcgcgg aaaggaaccg catgcagccc accgggtctg gaattcgttt
     1741 gtcgcgaagt atcggccatc cgcccagttc gtggtagctt ggcgtctgcc ccggccttcc
     1801 ggcttcgggc accccaacca tgtccgattc atcgcacgac tgcatccgcg tacgcggggc
     1861 ccggcagaac aatctccgtg gtctcgacct cgacatcccc ctgcgacagc tggtcgtcgt
     1921 gaccggcgtg tccggatcgg ggaagtcctc gctcgtcttc gacacggtct atgcggaagg
     1981 ccagcgccgc tacgtcgaga ccttctcgcc gtacgcgcgg cagttcctgg atcgcatgga
     2041 caagccgcag gtcgaccgca tcgacggcat cccgccggcg atcgcgatcg accagaccaa
     2101 tccggtgcgc acgtcccgct ccacggtcgg gaccatgacc gagcttaacg accatctgaa
     2161 actgctgttc gcgcgcgcgg cgcagctgca ctgccgtggc tgtggcgggc cggtgcgtcg
     2221 ggacactccg gactcgatct atgcggcctt gacggatcgc gccgcgccgc tcggcgatcc
     2281 gcggctggtc gtgacgttcc cggtgaccgt cccgccgagt ttcaccgagg ccgaagtcgt
     2341 gaaactgctg gagcagcagg gctacacgcg catccactcg cgccagccgg cccacgtcga
     2401 ggtcgtgcag gaccgcttca ggctcggggg cgcggagcgc gcacgcgtga tcgacgcgct
     2461 cgaatcggcg ctgcgggtcg gtcgtggccg cgtgagtgtc cgtacctctc ccgtctctcc
     2521 ctcgaaggag gcagaggtcg cgcgcagcgc gggtgagggc ccggcagatc gacagccctc
     2581 acccgccggc gcgggggagg gagaggcgga agaggtctgg cgcttctcga ccgacctgca
     2641 ctgcgccgac tgcgacatcc attatcgcga gcccgccccc agcctgttct cgttcaactc
     2701 gccgctcggg gcgtgcgaga cctgccgcgg gttcggccgc gtgatcggca tcgactacgg
     2761 gctggtggtg cccgacgtcg cgaagacgct gcgcgggggc gccgtgaagc cctggcagac
     2821 cgaatccaac cgcgagtgcc aggacgacct gctgcgctat gcgaagaagc gcgacgttcc
     2881 cgtcgacgtg ccgtggcgcg acctgacgcc ggaccagcag cgctgggtgc tcgagggcga
     2941 aggcccctgg gagaagaagg tctggtacgg cgtgcgccgc ttcttccagt ggctcgaatc
     3001 gaagagctac aagatgcacg tgcgggtgct gctcgcgaag taccgcagct acacgccgtg
     3061 tccgacctgc cacggcgccc ggctcaagga cgaggcgctg ctctggcggc tcgccacgtc
     3121 cgaccggccg gacgcggcgc tgaacatcca ccaggtgatg ctgctgtcgc tggcggactg
     3181 cctcgggttc ttcgagcgcc tcgcgctgcc ggcgccgctc gacgaggcga ccgcgcagct
     3241 gctcgacgaa gtccgcgcgc gcctcggatt cctggtgcgc gtcggcgtcg gttacctgac
     3301 gctcgaccgc cagtcgcgca cgctgtccgg tggcgaggtc cagcgcatca acctgaccac
     3361 ggcgctcggc acctcgctgg tcaacacgct gttcgtgctc gacgagccgt cgatcggcct
     3421 gcacccgcgc gacatgaagc gcgtgatcga ggtgatgcat cgcctgcgcg acaacggcaa
     3481 ctcgctgctg gtcgtcgagc acgatccgca gatcatgctg acggccgacc gcatcgtcga
     3541 catgggaccc ggaccgggtg agcacggcgg tcgagtggtg ttcgagggca cgccggccga
     3601 gctggccgtg cgatcggaca cgttgacggc gcaatacctg tccggccgtc gcgacgcggg
     3661 tgtctcggcg ggccggcgtg cgccaccgcg gccgggcgac ccggtcatcc gcgtatgcgg
     3721 ggcgacggag cacaacctgc acgacctcga cgtcgagttt ccgctgcacc ggctcgtctg
     3781 cgtgaccggc gtgtcgggct ccggcaagtc gacgctggtg caggacgtgc tgtacgcggg
     3841 gctcaggcgc gccaagggca ggccgacgga gacgccgggt gcgcatcgcg aaatcgtcgg
     3901 tgcggagctg atcgacgacg tcgtgatggt cgaccagacg ccgatcgggc gcaccacgcg
     3961 ctcgaacccc gcaagctacg tgggcgcatt cgaggcgatc cgcaagcggt ttgcgcgcgc
     4021 tccgctcgcg ctcgagcgcg gctacacggt cggcacgttc agcttcaact ccggcaacgg
     4081 ccgctgtccg acctgcggtg gcaacgggtt cgagcacgtc gagatgcagt tcctgtcgga
     4141 cgtgtacctg cgctgccccg attgcgacgg caagcggttc cgccccgagg tgctcgaggt
     4201 gcgcgtcgag cgccacggcc gctcgctcga cgtcgcgcag gtgctcgagc tcaccgtgtc
     4261 cgaagccgtg cagttcttcg ccgaagacgc cgacgtactg gcgcggctcg agccgctcgc
     4321 ggccgtgggc ctcgactacc tgcggctcgg ccagccggtg ccgacgctgt cgggcggcga
     4381 ggctcagcgg ctgaagctcg cgggtttcct ggcggacgtg ggggaacgtc cggaaaaaag
     4441 cggacagtcc ccgttttccc agagcggaaa agggggacag tcccgcacgc tgttcctgtt
     4501 cgacgagccg acgacggggc tgcacttcga ggacgtctcg aagctgctcg aggcgttcaa
     4561 gcggctgctg gacgccggtc actcgctgct cgtgatcgag cacaacctcg acgtgatccg
     4621 cgcctccgac tggatcgtcg acctgggacc cgagggcggc gacggcggcg gtcgcctgat
     4681 ggtggccggc acgccgcaga cggtgatggc ggcgacgaat tcgcacacgg gtcacgccct
     4741 gcgggagtac gagcgggagc gcgccgaggt ggtccgcgcg gggcaggcgc gactgccgtc
     4801 gttcacgctg ccggcgacga agcgtgacgc ggtggcggac cgcgcgcgcg actcgatcgt
     4861 cgtgcaccgc gcgcgcgagc acaacctgcg tgacatcgag gtcacgatcc cgcgcgagtg
     4921 catgacggtg ctgaccgggg tctcgggctc cggcaagtcg acgctcgcgt tcgacatcgt
     4981 gttcaacgag gggcaacggc gttacctcga gtcgctcaac gcctatgcgc gccagttcgt
     5041 ccaaccggcg gctcgaccgg acgtcgatgc gatcttcggc atcccgccga ccgtcgcgat
     5101 cgagcagcgc accagccgtg gcggccgcaa gagcaccgtc ggcacgttga ccgaggtgca
     5161 tcacttcctg cggttgctgt acgtcaagct gggtacgcag ttctgcccgg attgccaggt
     5221 gccgatcgag ccgcagagcg cggactcgat cgcggcgctg ctgttgcgcg aggcgcgcgg
     5281 acgccggatc gagctgctcg cgccgctcgt ggtcgcgcgc aagggcgtct acacggacct
     5341 cgcgaagtgg gccgcgtcga aaggctacgc ggagctgcgc gtcgatggcg cgatgctgcc
     5401 cacggcgaag tggccgcggc tcgaccgctt ccgcgagcac acgatcgagc tgcccgtcgg
     5461 cgcactgaag gtgacgcccg agcacgaagc cgggttgcgt gcgacgctcg accgcgcgct
     5521 cgagttcggc aagggggtcg cacacgtcgt cgatcgcggc aagacgcgcg tgttctcgac
     5581 gttgcgtgcc tgcccgtcgt gcgggcgcag cttcgccgag ccggacccgc gcatgttctc
     5641 gtacaactcg aagcacggct ggtgtgacgg ctgctacggc accggactgc agatcagcgg
     5701 cttcgacgcc gagcagtcgg gcgaggaagt ttggtggaac gaatggttcg agggcgaggc
     5761 ggccgcctgt acgagctgcg acggtcgccg actcaacccg gtcgcgctca acgtccgatt
     5821 ccgggggttg tcgatcgcgg acctctcggc actgccggtg gagcaggcac tggcgttctt
     5881 cgaagcgctc gtcctcacgg gacgcgaggc cgagatcgca cgcgacctgc gcgcggaggc
     5941 cgggtcgcgg ctcgtgttcc tgcgcgacgt cgggctcggc tacctctcgc tcgatcgctc
     6001 ggcgccgacg ctgtccggcg gcgaggcgca gcgcat
//
LOCUS       DASPFR010000125         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_158882, whole genome shotgun sequence.
ACCESSION   DASPFR010000125 DASPFR010000000
VERSION     DASPFR010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158882"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..1476
                     /locus_tag="VD737_03155"
     CDS             382..1476
                     /locus_tag="VD737_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HXW09585.1"
                     /translation="MNERPDRRAEDVRKGQGDVKLSREEFERRLRERFYDPAFGSLSA
                     DLDRVVDTAWRNYDEYHKSPRTRRAGAGFADPSFELPVEWLEARAAIEAAQRRHDDTS
                     GPARILLVCGAARHDQTCPGEMSKTFRLASAARQAMEQPDVDCDLLDLSLLTAQYGRQ
                     ILPCKACVSTAMPLCHWPCSCYPNHAMGQVNDWMNELYPRWVAAHGVMIVTPVYWSQA
                     PSVLKLMIDRLVCADGGNPDPTTTRGKDPVRAKALELGGWPYPRHLAGRLFAVVVHGD
                     AEGTDILRRSLTGWLQDMHLIQAHPVATIDRYVGYYEPYATSHAALDRDDAVVEEVRQ
                     AARTLVEAVKLARSGLLVQVQPPGAEQRPK"
     gene            complement(1508..2002)
                     /locus_tag="VD737_03160"
     CDS             complement(1508..2002)
                     /locus_tag="VD737_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09586.1"
                     /translation="MTLQDWGSIGEVVGALAVVVTLIYLAKQIKQNTHAMEEGRRLAL
                     AQTYQMRSDALQAMLVQAADSDHIGPIIIKLTEKGYPEDVGALGDLTEAELRRFRMWQ
                     IAQQTHWDNMHYQYQQGYLDPEYYEDAFKERVVRLAPTWRALGLTGGRRSFFAEIERL
                     MKSS"
     gene            2184..>2471
                     /locus_tag="VD737_03165"
     CDS             2184..>2471
                     /locus_tag="VD737_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09587.1"
                     /translation="MSEGRAITDGATREGFSGGWTGIVAVALFAYVALAIGLILVDGQ
                     GGRALEIFNLYSDSVASIVVAVLAGAAAHGSQHPAMRRTWWQLTAALGCYSA"
BASE COUNT          380 a          860 c          830 g          401 t
ORIGIN      
        1 agcccgactt gcgactggaa cccgacttct tcttgcgtgg agggaccttt ccgccttcac
       61 gccgggcctc ggacaggccg atggcgatgg cctgtttgcg actcttcacc gtgcgtccgg
      121 acccgctctt caacgtgccg cgcttgcgct tgtgcatcgc gcgcgccacc tttcgcttgg
      181 atttcgcgcc gtaacgactg gccatcgctg cacctcgcac tcgtcgtggg gaggcaaacg
      241 atcgcatgca cgcaaacata agcgctgtcg gcggaggcgt gaccggccgc tcgcccaccg
      301 cgcacgcggc tatcggcgct cgtgcaggtg cctgccccga ccgcaccgaa ccatcgcggg
      361 ccgcccgcat ctaaccgtgt catgaatgaa cggcccgacc gtcgagcgga ggacgtgcgc
      421 aagggccagg gcgacgtcaa gctgagccgc gaggagttcg agcgccggct gcgcgagcgc
      481 ttctacgacc ctgcgttcgg gtcgctgtca gccgacctcg accgtgtcgt cgacaccgcc
      541 tggcgcaact acgacgagta ccacaagagc ccgcgcacgc gacgagctgg cgcagggttc
      601 gccgatccgt cattcgagtt gcctgtggag tggctcgagg cccgggccgc gatcgaggcc
      661 gcgcaacgac gtcacgatga tacgagcggg cctgcgcgca tcctgctcgt ctgcggcgcc
      721 gcccgacacg accagacctg ccccggcgag atgtccaaga cgttccggct cgcaagcgcc
      781 gcccgtcagg cgatggaaca gccggacgtc gactgcgatc ttctggacct gagcctgctc
      841 acggcgcagt acggccgcca gatcctgccc tgcaaggcgt gcgtgtcgac tgccatgccg
      901 ctctgtcact ggccctgctc gtgctatccg aatcacgcaa tggggcaggt caacgactgg
      961 atgaacgaac tgtacccgcg gtgggtggcc gcgcacggcg tcatgatcgt gactcccgtg
     1021 tattggtccc aggcgccgag cgtgctcaag ctgatgatcg accggctcgt gtgcgccgac
     1081 ggcggcaacc cggacccgac cacgacgcgc ggcaaggacc ccgtgcgggc caaggccctg
     1141 gaactcggcg ggtggcccta tccgcgacac ctggcggggc ggctgttcgc ggtggtcgtg
     1201 cacggtgacg ccgagggcac cgatatcctg cgtcgttcgt tgacgggctg gctgcaggac
     1261 atgcacctga tccaggccca cccggtcgcg acgatcgatc gctacgtcgg ctactacgaa
     1321 ccgtacgcga cgagccacgc cgcgctcgat cgggacgatg ctgtcgtcga agaggtgcga
     1381 caggccgcac ggacactggt cgaggccgtg aagctcgcgc gctccgggct gctcgtgcag
     1441 gtgcagccgc cgggcgcaga gcaacggccc aagtgatgcg cgcgtggtcg gcgcgcaggc
     1501 gggcgcccta cgacgacttc atcagccgtt cgatctccgc gaagaaactg cggcggccac
     1561 cggtcaggcc gagggcacgc caggtcggcg cgagccgcac gacccgctcc ttgaacgcgt
     1621 cctcgtagta ttccgggtcg aggtaaccct gctggtactg gtagtgcatg ttgtcccagt
     1681 gcgtctgctg ggcgatctgc cacatgcgga agcggcgcag ttcggcctcg gtcaggtccc
     1741 cgagcgcgcc gacgtcctcg ggatagccct tctccgtcag cttgatgatg atcggcccga
     1801 tgtggtcgga atcggccgcc tgcaccagca tcgcctgcaa cgcgtcggaa cgcatctggt
     1861 aggtctgggc gagcgcgagg cgacggccct cctccatcgc gtgcgtgttc tgcttgatct
     1921 gcttggcgag gtagatgagc gtgacgacca cggccagcgc cccgaccacc tcgccgatcg
     1981 atccccagtc ctgcagcgtc atcacgcacc tcccatcgac ggtcccgacc gccacgatag
     2041 cccagcgcgg ccacgacccg cattgtgaac tccgtcacac cgcggcccgt gggatccgga
     2101 ccggtgtcac ggtcgccgcc cggcgatgtc aggctgaata tcgcgggttt atgtaatccc
     2161 ggcgctcggg gaggcgccgc gtcatgtccg aagggcgagc gatcaccgac ggcgccacgc
     2221 gcgagggctt cagcggcggc tggaccggca tcgtggccgt ggcactgttc gcctacgtcg
     2281 ccctcgcgat cgggctgatc ctggtcgacg gacagggcgg ccgggcgctg gagatcttca
     2341 acctctactc ggattcggtc gcgagcatcg tggtggccgt gctggcgggc gcggcggcac
     2401 acgggtcgca gcaccccgcc atgcggcgca cctggtggca gctcacggcc gcgctcggtt
     2461 gctacagtgc c
//
LOCUS       DASPFR010000126         3285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1135602, whole genome shotgun sequence.
ACCESSION   DASPFR010000126 DASPFR010000000
VERSION     DASPFR010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135602"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..1485)
                     /locus_tag="VD737_03170"
     CDS             complement(301..1485)
                     /locus_tag="VD737_03170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002751.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HXW09588.1"
                     /translation="MTDAPRAILHVDMDAFYASVEQRDDPALRGKPVIVGGGGNRGVV
                     AAASYEVRVFGVHSAMPTREALRRCPHAIVVPPRISHYSAVSRQIFAVFHEFTPLVQG
                     LSLDEAFLDVTASQGALGSAETIAREIKRRIRDRTQLTASVGVAPNKLVAKIASDLRK
                     PDGLVVVQAADVRELLDPLPIRRLFGLGPKTAPRVEALGIHTLGDLRQASPARLRPLF
                     GRYTERVQQRAAGIDDRPVIPDEDEKQISAEETFDTDVADRAWLRAEIARLADRACSR
                     LRARGLVGGCVTVKIRRGDFATYTRQRAFEPRTEETRVVTRIATELLDAWLATQPRAA
                     LRLLGVGVSDLAVASQGDLFAAAESTRNRQLDAAVDEIRGRFGPIALQPASSVRSRRN
                     GA"
     gene            complement(1496..3031)
                     /locus_tag="VD737_03175"
     CDS             complement(1496..3031)
                     /locus_tag="VD737_03175"
                     /inference="COORDINATES: protein motif:HMM:NF025479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HXW09589.1"
                     /translation="MPRAEEQETVIYEVNLEADAAIEAPFDTWLRDHVADMLQLPGFL
                     SAEVLVDVAAPSGRVRRTVQYRLRSQADLDRYLREHAPRMRQHGVALFGERFTAERRT
                     LAHREEFIRGQVSTENCLNCGEVLTGQHCSHCGQRAKVRVLSLGSLLRDLFGDLTEFD
                     SRIWRTLIPLAFRPGLLTAEYLRGRRTHYTPPFRMYLVLSVVFFLATSLGANPGDAMR
                     LNVGEDGANLQIGTDSGQDAGSGASMQGAADQADSTAASGPAASAPAPPGATAQGPTT
                     PAATPPGDAAAAGERERQRVIDAIVSRVPRAEQDRVRRELEREIAGATPEELETVGRI
                     VEDPCSESNVQIQLGSWGSKYEPRLREACRKIVSDSSSFGRALYENIPKMMFIFLPLI
                     AAVMFTLYLGSGRYYVEHLLFFVHYHAFFFLGGLAIVVLDRSSALLEGTVFGNVLRFF
                     EGALTFALAIYVPYYLYRAMRRVYGQGRMVTLAKYSLLGIGYVFFMALTAVGLLFYTA
                     LTL"
BASE COUNT          519 a         1148 c         1155 g          463 t
ORIGIN      
        1 cagagagcgt agaacccagg gccacggtga cgaccaggtc gaacgcgttc atcttcgaca
       61 acgtgcgatt gccggacacg cgcagcaggc acaccagtgc gacgtaggcc agcacgccga
      121 cgacgagcgt gcgcagcagg ctgtcccagc tgctgaacag gaatgtgtcc atgggctgct
      181 ccgttccatc ggtcgccggc gggtcgcccg gccgggagac cgtaccaagc ctgccgaccc
      241 gaccctgtcc gtgagtcccc gacctcgacg tctcaggccg gctcgaacga cccgcgctcg
      301 ctacgcgccg ttccgtcggc tgcggaccga gcttgccggc tgcagcgcga tcgggccgaa
      361 gcggccgcgg atctcgtcga ccgcggcatc gagctggcga ttgcgcgtcg actccgcggc
      421 cgcgaacagg tcgccctgcg aggcgacggc gagatcgctg acaccgacgc ccagcagccg
      481 caacgcggcg cgaggctgcg tggcgagcca cgcgtcgagc agctcggtgg cgatgcgcgt
      541 cacgacgcga gtctcctcgg tgcgcggctc gaacgcgcgc tggcgcgtgt aggtcgcgaa
      601 gtcgccacgc cggatcttga ccgtgacgca gccgccgacc agcccgcgcg cgcgcagccg
      661 cgagcaggcc ctgtccgcga gccgggcgat ctccgcgcgc agccacgcgc ggtcggcgac
      721 gtcggtgtcg aaggtttcct cggcgctgat ctgcttctcg tcctcgtccg ggatcaccgg
      781 gcggtcgtcg atgccggcgg cgcgctgctg cacgcgctcc gtgtaacgcc cgaacaacgg
      841 tcgcagccgc gccggcgagg cctgacgcag gtccccgagc gtgtggatgc cgagcgcttc
      901 gaccctcggc gccgtcttcg ggcccaggcc gaacagtctg cggatcggca gggggtcgag
      961 cagctcacgg acgtctgccg cctgcaccac gacgagtccg tccggcttgc gcaggtccga
     1021 ggcgatcttc gcgacgagct tgttcggggc cacgccgacc gacgccgtca gctgcgtgcg
     1081 gtcgcggatc cggcgcttga tctcgcgcgc gatcgtctcg gcgctgccga gcgcgccctg
     1141 gctcgcggtc acgtccagga acgcctcgtc gagcgaaaga ccctgcacga gcggcgtgaa
     1201 ttcgtggaac accgcgaaga tctgcctcga gacggcgctg taatggctga tccgaggcgg
     1261 caccacgatc gcgtgggggc agcggcgcag cgcctcccgc gtcggcatgg ccgagtgcac
     1321 gccgaagacc cgcacctcgt aactggctgc ggcgaccacg ccgcggttgc cgccaccacc
     1381 gacgatgacc ggcttgccgc gcagcgcggg atcgtcgcgc tgctcgaccg acgcgtagaa
     1441 cgcgtccatg tcgacgtgca ggatggcgcg cggcgcgtcg gtcatcacgg cctgctcaga
     1501 gcgtgagcgc agtgtagaag agcaggccga ccgccgtcag cgccatgaag aacacgtagc
     1561 cgatgccgag cagcgaatac ttggcgagtg tgaccatccg cccctggccg tagacgcgcc
     1621 gcatggcgcg gtagaggtag tacggcacgt agatggccag ggcgaacgtg agcgcgccct
     1681 cgaagaaccg cagcacgttg ccgaacaccg tgccttccag cagtgcggag ctgcgatcga
     1741 gcaccacgat cgcaagcccg ccgaggaaga agaaagcgtg gtagtgcacg aagaacagca
     1801 ggtgctcgac gtagtagcgc cccgagccga ggtacagcgt gaacatcacg gccgcgatca
     1861 gcggcaggaa gatgaacatc atcttcggga tgttctcgta gagcgcccgg ccgaacgacg
     1921 aggaatcgct gacgatcttg cggcaggctt cgcgcaggcg cggctcgtac ttgctgcccc
     1981 acgagccgag ctggatctgc acgttcgact cgctgcacgg gtcctcgacg atccggccca
     2041 cggtctcgag ttcctccggc gtggcaccgg cgatctcgcg ctcgagctcg cgtcgcacgc
     2101 gatcctgctc cgcccggggc actcgtgaga cgatcgcgtc gatcacgcgc tggcgctcac
     2161 gttcgccagc cgccgcagcg tcgccagggg gggtcgccgc aggcgtggtc ggtccctgcg
     2221 ccgtcgcccc gggtggcgcg ggcgccgacg cagcggggcc gctcgctgcc gtcgagtcgg
     2281 cctggtcggc ggcgccctgc atggacgcgc ccgagccggc gtcctggccg gagtcggtgc
     2341 cgatctgcag gttcgcaccg tcctcgccca cgttcagccg catggcgtcg cccgggttcg
     2401 cgcccagcga ggtggcgagg aagaacacga cgctcagcac gaggtacatc cggaacggtg
     2461 gcgtgtagtg cgtgcgacgg ccgcgcaggt attcggccgt gagcaggccg ggtcggaacg
     2521 cgagcgggat caacgtgcgc cagatccgcg agtcgaactc ggtcaggtcg ccgaagaggt
     2581 cgcgcagcag gctgccgagc gacagcacgc gcaccttggc gcgctgcccg cagtgcgaac
     2641 agtgctgccc ggtgagcacc tcgccacaat tgaggcagtt ctccgtggac acctggccgc
     2701 ggatgaactc ctcgcggtgc gccaacgtgc ggcgctccgc cgtgaaccgc tcgccgaaca
     2761 gcgcgacgcc gtgctggcgc atccgcggcg cgtgctcgcg caggtagcgg tcgaggtcgg
     2821 cctggctgcg caggcgatat tgcacggtgc gccgcacgcg cccggacggc gccgccacgt
     2881 cgaccaggac ctcggcggac agaaatcccg gcaactgcag catgtcggcc acgtgatcgc
     2941 gcagccaggt gtcgaacgga gcctcgatcg ccgcgtcggc ctcgaggttg acctcgtaga
     3001 tgaccgtctc ttgttcttcg gcccgcggca ccgcgcaact ctatcgcaaa gcggagcggc
     3061 cgtcggagcg atcatggccg cgacgggaat cgctctgtac catcgcccgc acgcacggag
     3121 gaaacatgcc gcaactgcag gccaacgggg cgacgttcga agtggtcctg gacggacccg
     3181 tcgacgcgcc gtgcctggtg ctgtccaact cgctcgggtc gtcgcacgag atgtggatac
     3241 gccaggtcga gccgttcgcg cggcacttcc gcgtgctgcg ctacg
//
LOCUS       DASPFR010000127         2320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_891576, whole genome shotgun sequence.
ACCESSION   DASPFR010000127 DASPFR010000000
VERSION     DASPFR010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891576"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VD737_03180"
     CDS             <1..712
                     /locus_tag="VD737_03180"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HXW09590.1"
                     /translation="ALDAGLSGPGRLHDLFVGLEAVTPGEYKSRGRGLTMRHGTADTP
                     FGPARIALTERGIAYLAFADEHGQFDGWEAFRNTWREADWCEDDRAAASIAATIWRTA
                     PGTTRKLSLWLHGSNFQLQVWRALLAAGARSTTSYAELARDIGNPSACRAVGAAVGSN
                     PVAWLIPCHHVLRANGALSGYRWGPARKRAMLAWELARSLRGASRPLRVTSSRASTAS
                     ARSASTPASSRALRGSIA"
     gene            complement(600..1091)
                     /gene="phaR"
                     /locus_tag="VD737_03185"
     CDS             complement(600..1091)
                     /gene="phaR"
                     /locus_tag="VD737_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HXW09591.1"
                     /translation="MTQERLIKKYANRRLYDASQSRHITLDDIRGLIVKGEKIKVVED
                     KTGNDITRHILLQVIAEQEQFGRPILSTTVLESIIRFYGNSMQGFLASFLEKSVETFL
                     HQQDNLQQQISKIVSSAPIATMADLTRSNLEALSKMQESMLSALMPSKREKTSREEDE
                     KRP"
     gene            complement(1189..1536)
                     /locus_tag="VD737_03190"
     CDS             complement(1189..1536)
                     /locus_tag="VD737_03190"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HXW09592.1"
                     /translation="MPAKTSSTADLGDFAKRAFTPATRLNETAVANFERIARFQYDLT
                     GDLLQFGLDQLQASAKAKDLTTLISKQRELATKFVEKAQARQKDLAEIASESQADVAK
                     WIEDATALALRKA"
     gene            1736..2209
                     /locus_tag="VD737_03195"
     CDS             1736..2209
                     /locus_tag="VD737_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXW09593.1"
                     /translation="MSSQQQAVLEILKGRIGEEIHLSDWLPVSQERINEFAAATGDFQ
                     WIHVDVERASRESPYGGTIAHGYLTLSLYPLLRGLVDASTPLFPGVQRVINYGLDKLR
                     FPNAVRAGSRVRARCVLLKVDEVAGGLQMHEQYTVEIDGEAKPACVAEAIMRVYF"
BASE COUNT          371 a          753 c          797 g          399 t
ORIGIN      
        1 cgcgctcgac gcgggacttt caggcccggg ccggctgcac gacctgttcg tcggcctgga
       61 agccgtgacg cccggcgaat acaagtcgcg cggacgaggc ctcacgatgc gtcacggcac
      121 ggccgacacg ccgttcggac cggcgcggat cgcgctcacg gagcggggga tcgcctacct
      181 ggcgttcgcg gacgagcacg ggcagttcga cgggtgggag gcgtttcgca acacctggcg
      241 cgaggcggac tggtgcgagg acgatcgcgc ggcggcgagc atcgccgcca cgatctggcg
      301 cacggcgccg ggaacgacgc gcaagctgtc gctgtggctg cacggcagca acttccagct
      361 gcaggtctgg cgggcgctgc ttgccgcggg cgcgcgatcg acgacgtcat acgcggagct
      421 cgcccgcgac atcggcaatc cgtccgcatg ccgcgccgtc ggtgccgccg taggctcgaa
      481 cccggtcgcg tggctgatcc cgtgtcacca cgtcctgcgg gccaacgggg cgctgagcgg
      541 ctaccgctgg gggccggcgc gcaagcgcgc gatgctcgcc tgggagctcg cgcggtcgct
      601 caggggcgct tctcgtcctc ttcgcgtgac gtcttctcgc gcttcgacgg catcagcgcg
      661 ctcagcatcg actcctgcat cttcgagagc gcttcgaggt tcgatcgcgt gaggtcggcc
      721 atcgtggcga tgggcgcgga ggagacgatc ttggagatct gctgctgcag gttgtcctgc
      781 tggtgcagga acgtctccac gctcttctcg aggaagctcg cgaggaaccc ctgcatcgaa
      841 ttgccgtaga agcggatgat cgactcgagc acggtggtgc tcaggatcgg ccgcccgaac
      901 tgctcctgct cggcgatgac ctgcagcagg atgtggcggg tgatgtcgtt gcccgtcttg
      961 tcctcgacga ccttgatctt ctcgcccttg acgatcaggc cacggatgtc gtccagcgtg
     1021 atgtgacggc tctgcgacgc gtcgtagagc cgacggttcg cgtatttctt gatcaggcgt
     1081 tcctgggtca tgggcgcgga gtgtacctga cccctgctgc gctgcacgaa atgcatcgca
     1141 aaaagagcgg gcggcgaccg ggtcgccgcc cctcaaatac caggcaaatc aagccttccg
     1201 gagtgcgagc gccgtggcgt cctcgatcca cttggcgacg tcggcctgcg actcgctcgc
     1261 gatctcggcc aggtccttct ggcgggcctg ggccttctcg acgaacttgg tggcgagctc
     1321 gcgctgcttc gagatcagcg tggtcaggtc cttggccttg gcggaagcct gcagctggtc
     1381 gagcccgaac tgcagcaggt cgccggtcag gtcgtactgg aagcgggcga tccgctcgaa
     1441 gttggccacc gccgtctcgt tgaggcgggt ggccggcgtg aacgcgcgct tggcaaaatc
     1501 gccgaggtcg gcggtcgacg aagtcttggc gggcatggtg gtcggtcctt gcagctggtg
     1561 aatccaggcg tgatcctatc acgctcatat tgcagtgcaa tatccagcct atccgggatt
     1621 tccagaacat cggccacgtt ttaacgcggt gcaaaacgaa ccggatccaa cgggtcgagg
     1681 ccgcccgtgg ccgactggct taccattcca gcggagtcat taccggaagc gaaccatgag
     1741 cagccagcaa caggccgtcc tcgagatatt gaaagggcgg atcggcgagg agatccacct
     1801 gagcgactgg ctgccggtgt cgcaggagcg gatcaacgag ttcgcggccg cgaccggcga
     1861 tttccagtgg atccacgtcg acgtcgagcg cgccagccgc gaatcgccgt acggcggcac
     1921 catcgcgcac ggctacctga cgctatcgct gtatccactc ctgcgcggcc tggtggacgc
     1981 ctcgacgccg ctcttcccag gggtgcagcg cgtcatcaac tacggcctcg acaagctgcg
     2041 cttcccgaac gccgtccggg ccggcagccg cgtacgggcc cgttgcgtgc tgctcaaggt
     2101 cgacgaggtg gccggcggac tgcagatgca cgagcagtac accgtcgaga tcgacggcga
     2161 agcgaagccc gcctgcgtcg ccgaagcgat catgcgcgtg tacttctgac gcgcagaccg
     2221 cgtcggttcg atccgctaac ggtgcaggcg cgccgcccgg tccgggtccg gatccgagtc
     2281 cgcaagagac gccagcaaga cgcatccatg cgggctcggc
//
LOCUS       DASPFR010000128        13872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_525905, whole genome shotgun sequence.
ACCESSION   DASPFR010000128 DASPFR010000000
VERSION     DASPFR010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13872
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525905"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="VD737_03200"
     CDS             <1..1202
                     /locus_tag="VD737_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXW09594.1"
                     /translation="PIIADGVVVSGINGCERFKPEGCFITGHDPDSGRELWRTSTIAL
                     PGDPNDRTWGGVPVSRRGGGDNWFAGSYDPELKLFFLGTSQPKPWVAASRGMSPRDAA
                     LYTNSTLALEPRTGRLRWYFQHVPGETLDMETGFERVLVDVDGKPYLYTVGKDGILWR
                     LNRESGRFAGFAETLYQNLFEPLDHRTGKLRYRQDILDAKIGDPVAVCPSIYGGHNWQ
                     ASAYSPQAHSLIIPLHQLCVEMIGREVDLGEGGGGYGGESTVTPMPGSNGMLGRLSAY
                     DVRTLQPRWSHAQRAMFLTGVLTTAGGLAFVGDLDRWFKAFDVDTGKVLWQTRLGSGL
                     HGYPISYAAGGRQFVAVPTGMGVFRLMTARQSPDIYQPNGGNMLYVFELADQRFALQR
                     DLAQRGE"
     gene            1316..2401
                     /locus_tag="VD737_03205"
     CDS             1316..2401
                     /locus_tag="VD737_03205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXW09595.1"
                     /translation="MRAGLLMVFQNFMDTITDREAYERDLHLASLAEPLGFDTVSGVE
                     HHFFNYAMAPDPTQFLSYMAAKTERIGLLTGAVILPWWRDPIRVVEKMTLLDHLSKGR
                     ALFGIGRGLARREYDIFGIDMNEARDRFDEAADMIIKGLETGVVEGDGKYFKQKRTEV
                     RPGPYKSWKGRFYSVAMSSDSVPVVAKLGAKMMTFAQKPWDQMVPHFESYRALYMQHH
                     KAPPPPPVCVDFLCCHESAERAEALAREHMSNYYVTVMQHYEMASEHFKKMKGYGDYA
                     NNAEMLRSVGMEEAGKAFTDINTFGTPKQILEKLEQRRRTIGDFDLTVQVSYGGMSWK
                     DAESSIRLFAKEVLPEFQSWQPQVKAA"
     gene            complement(2471..2986)
                     /locus_tag="VD737_03210"
     CDS             complement(2471..2986)
                     /locus_tag="VD737_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09596.1"
                     /translation="MKLETVRRRAMAFDRRNGKDWPGAVRSAAMSGAIAAGATSAYVA
                     AAGARDTGSAVAPINATSHIVWGESAGDVESVDARHTLLGAALNLGACVFWAGFYERL
                     FGRAADRGRVGTALLGGGLVAAAAYVTDYHVVPKRLTPGWESRISQPSLLAAYAVLAL
                     SLPLRGLLRRR"
     gene            complement(3134..4789)
                     /locus_tag="VD737_03215"
     CDS             complement(3134..4789)
                     /locus_tag="VD737_03215"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXW09597.1"
                     /translation="MNTAPRPMHATMMDVPLSTQLIFAHGRRLHAGSRVANWRDGRFD
                     ATSFEDVARRVESLAGALAALGVRHNTRVATYCWNTRAHLECYLAVPSMGAVLHTLNV
                     RLFPEQILGIMNHAEDEVLVVDASLLPTLREVLPRVPTLRHVLVIGAAEAGADHFAPP
                     QARVHDYEQLLGRQEGRFDWPALAERDAAVVCYTTGTTGAPKGVVYSHRTIYLHTLGT
                     LGVDTFAISQADRILLLPSMFHANAWGLPYSAWMAGADLLMPGPDLKPASLRRMIERE
                     RPTFTAVVPTLINDLLQADRESPLDMTSFRVIVSGGSVVSPALIRAVRERWNVPVLQG
                     WGMTETSPMCCLSIPPRGTPVADEPAWRTRSGRPVPGMFVRLVGDDGRPLPNDGRTVG
                     ELQLRGPWVTGAYHLQASPESFTRDGWLRTGDVGTIDPQGYVQITDRSKDVIKSGGEW
                     VSSVDLENQIAYHPGVFEAAVIGVPDPRWDERPLAVVVRRPGEQVSAPELRNFLQERV
                     ARFWLPERWAFVDALPKTSVGKIDKKELRRQFGDGSIPVETVK"
     gene            complement(4786..5772)
                     /locus_tag="VD737_03220"
                     /pseudo
     CDS             complement(4786..5772)
                     /locus_tag="VD737_03220"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
     gene            complement(5816..6529)
                     /locus_tag="VD737_03225"
     CDS             complement(5816..6529)
                     /locus_tag="VD737_03225"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXW09598.1"
                     /translation="MTERWIVTGGSRGLGLAIARWARRRGHRVAVVARGVDEPALRSQ
                     LGSPSLAIAADVTVPESVKAAFDQVAAAWGGIDVLVNNAGLHRGGKADKLDLDDWDAV
                     LRTNLTGPLHCVRAALPHMGKGGSIVNIGAVVGFRGFPGDSPYGASKAGLAGLTRVLA
                     VELARQDIRVNLVVPGLVKTDMTEGLSGRAMDSIMKRIPMRRLGHDDEVAEVVGWVAG
                     STYMTGAIIPTDGGLSAML"
     gene            complement(6589..8832)
                     /locus_tag="VD737_03230"
     CDS             complement(6589..8832)
                     /locus_tag="VD737_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXW09599.1"
                     /translation="MHITHRSSRLATAVAMALGGAATTPPAAVAQQTAASGGGLEEII
                     VTARKREENLQDVATSVSALGAEEIARRFDVDLQSMSNAAPNVVIDDLQQGPGSPAAI
                     SIRGIGTTDVEKSFDPTTGVVVDEVFIGVNSGAMIKALDIVGMEILRGPQGTLFGRNS
                     IAGVINVTRGRPDPEDFGASVRLGYGNHNDQQFDGYVNVPLGENFAFKLGGAYRAHDG
                     WFYNRTLGKDVGDTEYKSVSPAFTWRPTEDLELYYRFDKSWQDQDANVVVNMAQADQA
                     FCFYYNQCAQGLESPQSGDRYVVLQNGDDPYQSYFDTDMHVANARWNLSDDSQVVYVF
                     GLFKTDEQVYQDWDATPLTLYHTDRPAEYEQTSHELRFTHTGERLSYTVGAYYWNSEY
                     NIDLLSYIGFGDLLFGLPAGTVLEVPQSVEQETDSTAVFFEGDWKFNDQWTLTVGGRY
                     TKDEKTSGVVDPIMPELADKGNPGNPFEESWSQFSPKVSLRYTYSDALMVYGLYSEGF
                     RAGGFSGRPGTYEGAATPYDPETVDNFEIGVKSEWLQGTLRLNASVFSMQYKDKQEEQ
                     SVPTDQGTGQQTLVLNASEAELNGIEFDFTWQATDMFRLAGNLGFLDASYKKLIDPVT
                     LTDLSDLELRRAPELTATISPTLEWPMLGGSMWVQADFRYIDEQELTFLNSPQSHVDA
                     QSIIDASINWRRDALTVSLWGLNLSEEDAWSQGYDVGTSVTFPGLWTYTATRPPRTYG
                     LRLMMNF"
     gene            9049..9531
                     /locus_tag="VD737_03235"
     CDS             9049..9531
                     /locus_tag="VD737_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXW09600.1"
                     /translation="MTNEALIEQLLQRVDRLESRQAMRDLVTDYCQAFDGVDFPRFLS
                     IWWDDCVWDIGPPFGRFDGHAGIEKAVKDVLWPVWRETHHLTSNLRVAFQDADNARGV
                     CDVDCMGSLRDDSVQMISATYTDHFQRRDGTWKILRRDVVIHYFNPIPGAQMTKPPAG
                     "
     gene            9552..10358
                     /locus_tag="VD737_03240"
     CDS             9552..10358
                     /locus_tag="VD737_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXW09601.1"
                     /translation="MGLLTVSEHRNVYFEDHAGPGLPVLLIHGWGMSCRVWDSTLVAL
                     LTAGHRVVTFDQRGCGQSDKDFAEVSVESSGADAVAVLRHLGVRRAAVNGWSLGGAVA
                     VEAARRLGADCAGVVLTGGASPRYVQAPDFPYGNPPGSPIEAVNALQLDRANFFDGLT
                     KAVCAIPQTDAMNAWMWSIFMQTSPAADRALAQLDALDQREAIRALECPLLAIVGGKD
                     AFVAPDIGRQAVKLARRGQLAEFPDCGHAPFIEDGPRYREVVLDFLRSLN"
     gene            10369..11379
                     /locus_tag="VD737_03245"
     CDS             10369..11379
                     /locus_tag="VD737_03245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXW09602.1"
                     /translation="MARQVNFGLWYDFRNPAPWAVPFERLYAESLEQIAGAEQLGFDS
                     IWMTEHHFCDDGYTPSPLVMAGAIGARTRRMRIGTNLMILPLHNPVRIAEDAATLSLL
                     TGGRFDLGVGIGYRELEFREFGRELKQRPSLIEEGIAIIRKAWAGERLNFEGKRFRVG
                     DVRITPVPEHLPKLYLGGMAEPAIDRAARIADGFLSTGGIGNDVYVKCLEKHGKRPED
                     GVIIAGHWALISDDPEREAAIVGPHALYQSNQYIEWGAFGPPETTPRFPDAATALQMG
                     LYELWDADTAVAKLTELLRTYPQIRDVHFWAQLPGEPLASGQRRIEYVAKHVLPRVRG
                     TL"
     gene            11390..11866
                     /locus_tag="VD737_03250"
     CDS             11390..11866
                     /locus_tag="VD737_03250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HXW09603.1"
                     /translation="MSLELRRAFGHYATGVTVITTTTPAGERIGMTANSFASLSLDPP
                     LVLWSIAKTSTNFDAFRGARNFAVHVLHSGQAALARQFATRQIDRFEGIACAVGRSGA
                     PLLCDYHACFDCETQEVIEGGDHLILIGRVLECDERAGEPLIFYRGRFLEGHTHGA"
     gene            11936..12679
                     /locus_tag="VD737_03255"
     CDS             11936..12679
                     /locus_tag="VD737_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXW09604.1"
                     /translation="MSRATKDEAAPRARRKQAERTALSDRKLTEAAIRLLVERGVQGT
                     TLQAVGELAGYSRGLATHRFGSKGGLFANVLEVASRDWLKRMQKAVGTRTGVEALCAA
                     TDAVEKFMRERPDELTVMYLLWFLGIDRSAEYQSNIAKVHKAQRRDVAAWIRAGQQAG
                     TVAADVEPLRVAEQYAASMAGIIYQWLADPGIPVGLMFAELKANLRHRLELPAHKAAR
                     LAEARSRKGSPERPKRAGNGNGRHAGAGR"
     gene            complement(12685..13506)
                     /locus_tag="VD737_03260"
     CDS             complement(12685..13506)
                     /locus_tag="VD737_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HXW09605.1"
                     /translation="MAAGGLVVAMFSEALAARRGNTPAVVPLGSFLDQLGTYDTQRWI
                     KADRWTNGSPFDNAWRADHVTHDGTAGVMSLRLSNRAYLGQPYSSGEYRTRGFYGYGC
                     YEARFQPVRQPGVVTSFFTFAGPYDNGGNGRHNEIDVEFLGYDTTIVQFNFWTNDDAY
                     GSHNERVLQLGFDASEAAHNYGFRWFEGGLEWYVDGSRVDSFLDTDVVRTPRPSDSYQ
                     KIMMNVWPVDDTASGWAGTFRYEGARTARYEWVRYDASPDCSFTYPAAAPKLGSS"
BASE COUNT         2140 a         4813 c         4773 g         2146 t
ORIGIN      
        1 gcccgatcat cgcggacggc gtcgtcgtga gcgggatcaa cggctgcgag cgcttcaagc
       61 ccgagggctg cttcatcacc ggtcacgacc cagacagcgg acgggagctc tggcgcacgt
      121 ccacgatcgc gcttcccggc gatcccaacg accggacctg gggcggcgta cccgtcagcc
      181 ggcgtggcgg cggcgacaac tggtttgcag gcagctacga ccccgaactc aagctgttct
      241 tcctcggcac gtcgcagccg aagccgtggg tggcggcgag ccgcggcatg tcgccgcgcg
      301 acgccgcgct ctacacgaac tcgacgctcg cgctcgagcc ccgcactggc cggcttcgct
      361 ggtacttcca gcacgtccct ggcgagacgc tcgacatgga gacgggcttc gagcgcgtgc
      421 tcgtcgacgt cgacggcaag ccgtacctct acaccgttgg caaggacggc atcctgtggc
      481 gactcaatcg cgaatcgggc cgcttcgccg ggttcgccga gaccctgtac cagaacctgt
      541 tcgagccgct cgatcaccgc acgggcaagc tcaggtaccg acaggacatc ctcgacgcga
      601 agatcggcga tccggtcgcg gtgtgtccga gcatctacgg cggccacaac tggcaggcct
      661 ccgcgtacag cccgcaggcg cactcgctga tcatcccgct gcaccagctc tgcgtggaga
      721 tgatcgggcg cgaggtcgac ctgggcgaag gcggcggggg atacggcggc gaatcgaccg
      781 ttacgccgat gcccggcagc aacggcatgc tcgggcggct gagtgcctac gacgtgcgga
      841 cgctgcagcc acgctggtcg catgcgcagc gcgccatgtt cctgaccggc gtgctcacca
      901 ccgccggcgg tctcgcgttc gtcggagacc tcgaccgctg gttcaaggcc ttcgacgtgg
      961 acacgggcaa ggtgctctgg cagacgcgtc tcggttccgg cctgcacggc tacccgatca
     1021 gttacgcggc cggtggccgg cagttcgtcg ccgtgccgac cggcatgggc gtgttccggc
     1081 tcatgaccgc ccggcaatcg ccggacatct accagccgaa cggcggcaac atgctctacg
     1141 tgttcgagct cgccgaccag cgttttgcgc tgcagcgcga cctcgcgcag cgcggcgagt
     1201 agtctcggcg ggacggccgc ccgcgctacg cttgctagtt gacaacaagt gtcctagcat
     1261 cggtcgcgct cgaggacgac tgccactcac ccgctccatc cagggggatc aggacatgcg
     1321 cgctggcttg ctgatggtgt ttcagaactt catggacacg atcaccgaca gggaggctta
     1381 cgagcgcgac ctgcacctcg cgagcctcgc ggaaccgctc ggtttcgaca cggtgagcgg
     1441 cgtcgagcac catttcttca actatgcgat ggcgccggat ccgacccagt tcctgagcta
     1501 catggccgcg aagacggaac gcatcgggct cctgaccggc gcggtgatcc tgccgtggtg
     1561 gcgcgacccg atccgtgtcg tcgagaaaat gacgctgctc gaccacctga gcaagggccg
     1621 cgccctgttc ggcatcggcc gcgggctcgc gcgccgcgaa tacgacatct tcggaatcga
     1681 catgaacgag gcccgcgacc gcttcgacga ggcggccgac atgatcatca agggcctcga
     1741 gaccggcgtc gtcgaaggcg acggcaagta cttcaagcag aagcgcaccg aggtccgccc
     1801 cgggccctac aagagctgga agggccgctt ctacagcgtg gcgatgtcgt ccgattcggt
     1861 gccggtcgtc gccaagctcg gcgccaagat gatgacgttc gcgcagaagc cgtgggacca
     1921 gatggtgccg cacttcgagt cctatcgcgc gctgtacatg cagcaccaca aggcgccgcc
     1981 tcccccgccc gtctgcgtcg acttcctgtg ctgccacgag agcgccgagc gggccgaggc
     2041 gctcgcgcgc gagcacatgt ccaactacta cgtcacggtc atgcagcact acgagatggc
     2101 gagcgagcac ttcaagaaga tgaagggcta cggcgactat gccaacaacg cggagatgct
     2161 gcgcagcgtc ggcatggagg aagccgggaa ggcgttcacg gacatcaaca ccttcggcac
     2221 cccgaagcag atcctcgaga aactcgagca gcgccgccgc acgatcggcg acttcgacct
     2281 caccgtgcag gtcagctacg gcggcatgtc gtggaaggat gccgaatcca gcatccgcct
     2341 gttcgctaag gaagtgctgc ccgaattcca gtcctggcag ccgcaggtca aggcggcctg
     2401 accgtactcc cctggcgcga cgcctcgaag ccgggctcgt cccggcttcg ttttgttcgt
     2461 cgcgcgcgat tcagcgacgg cgcagcaggc cgcgcagcgg caggctgagc gcgagcacgg
     2521 cgtaggccgc cagcagcgac ggctgcgaga tgcgcgactc ccagccgggc gtgagccgtt
     2581 tcggcacgac gtggtaatcg gtcacgtagg ccgccgccgc gaccagcccg cccccgagca
     2641 gcgccgtccc gacgcgaccc cggtccgcgg cgcggccgaa gagcctctcg tagaagcccg
     2701 cccagaacac gcaggcgccc aggttcagcg cggcgccgag cagcgtgtgg cgtgcgtcga
     2761 ccgactcgac gtcgccggcg gattcgcccc agacgatgtg actggtggcg ttgatcggcg
     2821 cgaccgcgct gccggtgtcg cgcgcgcctg cggccgccac gtacgccgac gtcgcgccgg
     2881 ctgcgatcgc gccggacatc gccgcgctgc gcaccgcgcc tggccagtcc ttgccgttgc
     2941 ggcggtcgaa tgccattgcc cgcctgcgca ccgtctcgag cttcatgtgc cacctccggt
     3001 tccagcaacg cgcaggattg cgaggcgcgc cgcgagcgtc tgcccgccgg gcaggacgac
     3061 ggcaggccgc tcggcggcgc cctgccgtgt aggtgaggca cgcacgcttg ctcggcgcgc
     3121 gcggctgcgc cggctatttc acggtctcga ccgggatcga gccgtcgccg aactgccggc
     3181 gcagctcctt cttgtcgatc ttgccgacgc tggtcttggg cagcgcatcg acgaacgccc
     3241 agcgctcggg cagccagaag cgcgcgacgc gctcctgcag gaagtttcgc agttctggcg
     3301 cactgacctg ctcgccgggt cgccgcacca ccacggccag gggccgctcg tcccagcgcg
     3361 gatcggggac gccgatcacc gcggcctcga acacgccggg gtgataggcg atctggttct
     3421 ccaggtccac cgacgagacc cattcgccgc cggacttgat cacgtccttg gagcggtcgg
     3481 tgatctgcac gtagccctgc gggtcgatgg tgccgacgtc gcccgtgcgc agccacccat
     3541 cgcgggtgaa cgactccggc gaggcctgca ggtgataggc gccggtgacc cacggtccgc
     3601 gcaactgcaa ctcgccgacg gtgcggccgt cattgggcag cggacgtccg tcgtcgccga
     3661 ccagccgcac gaacatgccc ggcacgggac ggccgctgcg ggtgcgccac gccggctcgt
     3721 ccgcgacggg cgtaccgcgc gggggaatgc tgaggcagca catcggactc gtctcggtca
     3781 tgccccagcc ctgcagcacc ggtacgttcc agcgctcgcg gaccgcgcgg atcagcgccg
     3841 gcgacaccac ggagccgccc gagacgatca cgcggaagct cgtcatgtcg agcggcgatt
     3901 cgcgatcggc ctgcagcagg tcgttgatca acgtcgggac cacggcagtg aacgtcggcc
     3961 gttcccgctc gatcatgcgc cgcaggctgg ccggcttgag gtccggcccg ggcatcagca
     4021 ggtcggcccc cgccatccac gccgagtacg gcagtcccca ggcgttggcg tggaacatcg
     4081 acggcagcag caggatgcgg tcggcctggc tgatcgcgaa cgtgtcgacg ccgagcgtgc
     4141 cgagcgtgtg caggtagatg gtgcgatggc tgtacacgac gcctttcggt gccccggtcg
     4201 tgccggtggt gtagcagacg accgccgcgt cgcgttccgc gagcgccggc cagtcgaagc
     4261 ggccctcctg ccgcccgagc agttgctcgt aatcgtgcac gcgcgcctgc ggcggcgcga
     4321 agtggtccgc gcccgcctcc gcggcaccga tcacgagcac gtgacgcagc gtcgggacgc
     4381 gaggcagcac ctcgcgcagc gtcggcagca gcgaggcgtc gacgaccagg acctcgtcct
     4441 cggcgtgatt catgatgccg aggatctgct ctggaaagag ccgtacgttc agcgtgtgca
     4501 gcaccgcgcc catcgaaggc accgccaggt agcactcgag gtgcgcgcgg gtgttccagc
     4561 agtaggtcgc gacacgcgtg ttgtgccgga cgcccagggc ggcgagcgct ccggcgagtg
     4621 actcgacgcg gcgtgcgacg tcctcgaacg acgtggcgtc gaagcgtccg tcgcgccagt
     4681 tcgcgacgcg actgccggcg tgcagccggc ggccgtgcgc gaagatcagc tgggtggaga
     4741 gcggcacgtc catcatcgtg gcgtgcatcg gccggggtgc ggtgttcatg agctgcggat
     4801 ccctgcgacc cgggcggcct cttcgagcag cgcgggcacg tcgtattcga gcttgcgcga
     4861 gtactcgctg tcgaccatgc cccgggcgtg cagcacgtac gcaccctcga cgatcgaggc
     4921 gaggcgccag aacgcgaaag cgaggtagaa gtcgaggcca gcgacgtcga tgccggttgc
     4981 gcgcgaccag cgcgccgcga gcgcctcgcg cgtcacgagt ccgggacgac gcgtgacggc
     5041 ctgcacgaac gcaaagccgg gcggctcgag cgcgcgcggt ccccagaaca tcagcatcag
     5101 cgccaggtcc gcgagtggat cgccgacggt cgcgagctcc cagtcgatga tggcggcgag
     5161 ccgcggcgcc gttcgatcga acagcgtgtt gtcgaggtga tagtcgccgt ggatcacggc
     5221 ctcgtgcgtg cgtggcggac ggtgccgttc cagtcaggcc gccaggcgct cgaccagcgg
     5281 caggtcacgc acggccgcgg cggcgcgcac ctcgcgccag cgcgcgatct cgcgcgatac
     5341 gtaatccggt ccggggttcg gcgcgcgcag tccgagcgtg cgccagtcga gccggtgcac
     5401 ttcggccagc gcgtcgacga gttcgtgccc gatgcgttcg agcgtcgcag gctcgtccgc
     5461 gtacgcttcg ggcagcgcga tcgtgatcgc cacgccttcc acgtgatcca cgacgaggaa
     5521 cggctggccg agcaccgacg ggtcggcgca gtaaccgcgg gccgccggca cccgtgcctt
     5581 gtgcgtgagc gcgctcagga tgcgccactc gcgctgcacg cccttggccg cgctgtcgga
     5641 cagtgcctgg tcgggcggtc gccgcagcac caggcggccg gccggcgtct ccaccagctg
     5701 cgtgacgttg gcgttgccgc cgctcagccg ggcgccgagt cgcgcgccgt cgatgccgag
     5761 ttcggcgcgc atccacgccg cgaagcgcgc gtccgtggcg gccgtcgcag gcacgtcaaa
     5821 gcatggcgga caggccgccg tccgtcggga tgatcgcgcc ggtcatgtat gtcgagccgg
     5881 cgacccagcc gaccacctcc gcgacctcgt cgtcgtgacc gaggcgtcgc atcgggatgc
     5941 gcttcatgat cgagtccatc gcgcggccac tcagtccttc ggtcatgtcc gtcttgacca
     6001 ggcccggcac gaccaggttc acgcggatgt cctgccgggc cagttcgacc gcgagcacgc
     6061 gggtcaggcc ggccagcccg gccttggacg caccgtaggg actgtcgccc gggaacccgc
     6121 ggaacccgac tacggccccg atgttgacga tggagccacc cttgcccatg tgcggaaggg
     6181 cggcccgcac gcagtgcagc ggcccggtca ggttggtccg cagcacggcg tcccagtcgt
     6241 cgaggtcgag cttgtcggcc ttgccaccgc ggtgcaggcc ggcgttgttg acgagcacgt
     6301 cgatgccgcc ccaagccgcg gcgacctggt cgaacgcggc cttgacggat tctggaacgg
     6361 tgacgtcggc ggcgatcgca aggctcggcg accccagctg ggagcgcagc gcgggttcgt
     6421 cgaccccgcg agcgaccacg gccacgcggt ggccgcgtcg ccgcgcccag cgtgcgatgg
     6481 cgagccccaa gccgcgggag ccgcccgtga cgatccagcg ctctgtcatg tggcgcgagc
     6541 cggcggtgcg cgacgccccg cggcggccgc gcaccgacgt gcccgtggtc agaagttcat
     6601 catcaggcgc aggccatacg tgcgcggcgg acgggtcgcc gtgtacgtcc acaggccggg
     6661 gaacgtcacg ctcgtgccga cgtcgtagcc ctgcgaccag gcgtcctcct ccgacaggtt
     6721 caatccccag aggctcaccg tcagcgcatc gcggcgccag ttgatcgacg catcgatgat
     6781 gctctgcgcg tcgacgtggc tctgcggcga attgaggaac gtgagttcct gctcgtcgat
     6841 gtagcggaag tccgcctgga cccacatgct gccgccgagc atcggccact cgagcgtcgg
     6901 cgagatcgtc gcggtcagct ccggcgcgcg acgcaactcg aggtcggaga ggtcggtgag
     6961 cgtgacgggg tcgatcagct tcttgtagct ggcgtcgagg aagccgaggt tgccagcgag
     7021 ccggaacatg tcggtcgcct gccaggtgaa gtcgaactcg atgccgttca actcggcttc
     7081 ggaagcgttc agcaccagcg tctgctgccc ggtgccctgg tcggtcggca cgctctgctc
     7141 ttcctgcttg tccttgtact gcatgctgaa caccgacgcg ttcaggcgca gcgtgccctg
     7201 gagccactcc gacttcacgc cgatctcgaa gttgtcgacc gtctccgggt cgtacggcgt
     7261 cgcggcgcct tcgtaggtgc ccggccggcc gctgaagcca cccgcgcgga aaccctccga
     7321 atagaggccg tagaccatca gcgcatcgct gtacgtgtag cgcaggctca ccttcggcga
     7381 aaactggctc cagctctcct cgaacgggtt gccgggatta cccttgtccg ccagctccgg
     7441 catgatcggg tcgaccacgc cgctcgtctt ctcgtccttg gtgtagcggc cgccgaccgt
     7501 cagcgtccac tggtcgttga acttccagtc gccttcgaag aacacggccg tcgagtcggt
     7561 ctcctgctcg accgactgcg ggacctcgag caccgtgccc gccggcagtc cgaacagcaa
     7621 gtcgccgaac ccgatgtagc tcagcaggtc gatgttgtat tccgagttcc agtagtaggc
     7681 gccgaccgtg taggagagcc gctcgcccgt gtgcgtgaag cgcagctcgt ggctggtctg
     7741 ctcgtactcg gccggacggt cggtgtggta gagcgtgagc ggcgtggcgt cccagtcctg
     7801 gtagacctgc tcgtcggtct tgaagaggcc gaagacgtag acgacctggc tgtcgtcgct
     7861 caggttccag cgcgcgttcg cgacgtgcat gtcggtgtcg aagtaggact ggtacgggtc
     7921 gtcgccgttc tgcagcacga cgtagcggtc gcccgactgc gggctctcga gcccctgcgc
     7981 gcactggttg tagtagaagc agaacgcctg gtcggcctgc gccatgttca cgaccacgtt
     8041 ggcgtcctgg tcctgccagc tcttgtcgaa gcgataatag agctcgaggt cctcggtcgg
     8101 gcgccaggtg aaggccgggc tgacggactt gtattcggtg tcgcccacgt ccttgccaag
     8161 cgtgcggttg tagaaccagc cgtcgtgagc gcggtaggcg ccgcccagct tgaacgcgaa
     8221 gttctcgccg agcggcacgt tgacgtagcc gtcgaactgc tggtcgttgt ggttgccgta
     8281 acccagccgg acgctcgcgc cgaaatcctc ggggtcggga cgcccgcggg tgacgttgat
     8341 cacgcccgcg atcgagttgc ggccgaacag cgtgccctgc ggtccgcgca ggatttccat
     8401 gcccacgatg tcgagcgcct tgatcatcgc gcccgagttc acgccgatga acacctcgtc
     8461 gacgaccacg ccggtggtcg ggtcgaaact cttctcgacg tcggtggtgc cgatgccgcg
     8521 gatggagatg gccgcggggc tgcccggccc ctgctgcagg tcgtcgatga cgacgttcgg
     8581 cgccgcgttg gacatcgatt gcaggtcgac gtcgaagcgg cgcgcgatct cctcggcgcc
     8641 gagcgcgctc acggacgtgg cgacgtcctg caggttttcc tcgcgcttgc gcgccgtgac
     8701 gatgatctcc tcgagtccgc caccggacgc cgcggtctgc tgcgcgactg ccgcgggcgg
     8761 tgtggtcgcc gctccgccca gcgccatcgc cacggccgtc gcgagacgcg aactcctgtg
     8821 cgtgatgtgc attgatcccc ctctccaaga tgcggatgcc gatcgatcgg accgtcgcca
     8881 cgcgcgcccc cggcaggggc cagcagcgcg gcacggggca gacgatagac tgcttgttgt
     8941 cggcaagcaa gtgttgctcc tatactcgac cgcgaccgcc ggacccttct tgcaacgcaa
     9001 cgccgtgccg cggtcgcgcc ggcgcaacgc cacggaaacc gttcgcccat gaccaacgaa
     9061 gcattgatcg agcagttgct gcagcgcgtg gaccggctcg agagccgcca ggcgatgcgc
     9121 gacctcgtca ccgactactg ccaggccttc gacggcgtgg atttcccgcg cttcctgtcg
     9181 atctggtggg acgactgcgt ctgggacatc ggtccgccgt tcgggcggtt cgacgggcac
     9241 gccggcatcg agaaggccgt gaaggacgtg ctgtggccgg tctggcgcga gacgcaccac
     9301 ctcaccagca acctgcgcgt cgcgttccag gacgccgaca acgcgcgcgg agtctgcgac
     9361 gtggactgca tgggctcgct gcgcgacgac tcggtgcaga tgatcagcgc cacctacacc
     9421 gatcacttcc aacgccgcga cggaacgtgg aaaattttgc gccgcgacgt cgtcatccac
     9481 tatttcaacc cgatcccggg cgcgcagatg accaagccgc ccgccggctg accagcgaca
     9541 ggggaacaca catgggcctg ctgaccgttt cggagcaccg caacgtctac ttcgaggacc
     9601 acgccgggcc gggcctgccg gtgctgctga tccacggctg ggggatgagc tgccgcgtct
     9661 gggactcgac gctcgtcgcg ttgctgacgg ccggccaccg cgtcgtcacg ttcgaccagc
     9721 gcggctgcgg gcaatccgac aaggacttcg ccgaggtgtc ggtggagtcg tccggcgcgg
     9781 acgcggtcgc ggtactgcgc cacctcggcg tccggcgggc ggcggtcaac ggctggtccc
     9841 tgggtggcgc ggtcgccgtc gaggcggcgc gccggctcgg cgcggattgc gcgggcgtcg
     9901 tactcacggg cggcgcttcg ccccgctacg tgcaggcccc cgacttcccg tacggcaacc
     9961 cgcccggcag cccgatcgaa gcggtcaacg cgctgcagct cgatcgtgcg aacttcttcg
    10021 acggcctcac caaggcggtc tgcgcgatcc cgcagacgga cgcgatgaac gcctggatgt
    10081 ggtcgatctt catgcagacc tcgccggccg ccgaccgcgc gctggcccag ctcgacgcgc
    10141 tcgaccagcg cgaggcgatc cgtgcgctcg agtgcccgct gctggcgatc gtcggcggca
    10201 aggacgcctt cgtggcaccc gacatcgggc gacaggcggt gaagctcgcg cggcgcggcc
    10261 agctggcgga gttccccgac tgcgggcatg cgccgttcat cgaggacgga ccgcgctacc
    10321 gcgaggtggt gctcgatttc ctgcggtcgc tgaactgagg ccgcggcgat ggcacgtcag
    10381 gtcaacttcg gcctctggta cgacttccgc aacccggccc cgtgggccgt gccgttcgag
    10441 cggctgtacg cggaaagcct cgagcagatc gcgggcgccg agcagctcgg cttcgactcg
    10501 atctggatga ccgagcacca cttctgcgac gacggctata cgccctcccc gctcgtgatg
    10561 gcaggcgcga tcggcgcccg caccaggcgc atgcgcatcg gcaccaacct gatgatcctg
    10621 ccgctgcaca accccgtgcg gatcgccgag gacgcggcca cgctctcgtt gctgaccggc
    10681 ggtcgcttcg acctgggcgt gggcatcggc tatcgcgagc tcgagttccg cgagttcggg
    10741 cgcgaactca agcagcgccc gagcctgatc gaggaaggca tcgcgatcat ccgcaaggcc
    10801 tgggcgggcg agcggctcaa tttcgagggc aagcgcttcc gcgtgggcga cgtgcgcatc
    10861 actccggtgc ccgagcacct gccgaagctt tacctcggcg gcatggccga gccggcgatc
    10921 gaccgtgcgg cccgcatcgc ggacgggttc ctctcgaccg gcgggatcgg caacgacgtc
    10981 tacgtgaagt gcctcgagaa gcacggcaag cgtcccgagg acggcgtcat catcgccggt
    11041 cactgggcgc tgatctccga cgacccggag cgcgaggcgg cgatcgtcgg tccgcacgcg
    11101 ctgtaccaga gcaaccagta catcgaatgg ggcgctttcg ggccaccgga gacgacgccg
    11161 cggttcccgg acgccgccac cgcgctgcag atgggactct acgaactctg ggacgcggac
    11221 acggccgtcg cgaaactgac cgaactgctg cgcacctatc cgcagatccg cgacgtccac
    11281 ttctgggcgc aactgcccgg cgagccgctc gcctccgggc agcgacgcat cgagtacgtg
    11341 gcgaagcacg tgctgcctcg cgtgcgcggg acgctgtgac cctgccgcca tgagcctcga
    11401 gttgcggcgc gcgttcgggc attacgccac gggcgtcacg gtgatcacga ccacgacgcc
    11461 cgccggcgag cgcatcggca tgacggcgaa cagcttcgcg tcgctctcgc tggacccgcc
    11521 gctcgtgctg tggagcatcg cgaagacgtc caccaacttc gacgcgttcc ggggcgcgcg
    11581 caatttcgcg gtgcacgtgc tgcattccgg gcaggccgcg ctggcgcgcc agttcgcgac
    11641 gcggcagatc gaccgcttcg agggcatcgc ctgcgcggtc ggccgcagcg gggcgccgct
    11701 gctctgcgac taccacgcgt gcttcgattg cgagacgcag gaagtgatcg aaggtggcga
    11761 ccacctgatc ctgatcggcc gcgtgctcga gtgcgacgag cgggcaggcg agccgctgat
    11821 cttctaccgc ggcaggtttt tggaaggaca cacgcacggc gcgtagaatg cgcgtcacgg
    11881 ctccgcgggg aggagctcac ttgttggcaa ccagcaagtc caatagaatg ccggcatgag
    11941 cagagccacg aaggacgaag ctgcaccgcg cgcacggcgc aagcaggccg aacgcactgc
    12001 cctctccgat cgcaagctca ccgaggcggc gatccggctg ctggtcgagc gcggcgtgca
    12061 gggcacgacg ctgcaggcgg tcggcgaact cgccggctac agccgcgggc tcgcgaccca
    12121 tcgcttcggc tccaagggcg gcctgttcgc caacgtgctc gaggtcgcga gccgcgactg
    12181 gctcaagcgc atgcagaaag ccgtcggaac ccgcaccggg gtcgaggccc tgtgcgcggc
    12241 caccgacgcc gtcgagaaat tcatgcgcga gcggcccgac gagctcacgg tcatgtacct
    12301 gctctggttc ctgggcatcg accgcagcgc cgagtaccag tccaacatcg ccaaggtgca
    12361 caaggcgcag cggcgcgacg tcgccgcgtg gatccgggcc ggtcagcagg cgggcaccgt
    12421 ggcggccgac gtcgagccgc tgcgcgtcgc cgagcagtac gcggcctcga tggccggcat
    12481 catctaccag tggctggcgg atccgggcat ccccgtcggg ctgatgttcg cggagctcaa
    12541 ggccaacctg cgccaccggc tcgagttgcc ggcgcacaag gcggcgaggc tcgccgaggc
    12601 gcgctcccgc aagggttcgc cggagcgccc gaagcgcgcc ggcaacggca acggtcgcca
    12661 cgcgggcgcg ggccgctagg gccctcagga cgacccgagt ttcggcgcgg ccgccgggta
    12721 cgtgaagctg cagtccgggc tcgcgtcgta gcgcacccac tcgtatcggg ccgtgcgcgc
    12781 gccttcgtac ctgaacgtgc cggcccagcc ggaggccgtg tcgtccacgg gccacacgtt
    12841 catcatgatc ttctggtacg agtccgacgg cctcggcgtc ctgacgacgt cggtgtcgag
    12901 gaacgagtcg acgcggctgc cgtcgacgta ccattcgagc ccgccctcga accagcggaa
    12961 tccgtagttg tgcgccgcct ctgacgcgtc gaagccgagc tgcagcacgc gctcgttgtg
    13021 ggagccgtac gcgtcgtcgt tggtccagaa attgaactgg acgatcgtcg tgtcgtagcc
    13081 gaggaactcg acgtcgatct cgttgtgcct gccgttgccg ccgttgtcgt acggaccggc
    13141 gaacgtgaag aacgacgtca cgacgccggg ctggcgcacc ggctggaacc tcgcctcgta
    13201 gcagccgtaa ccgtagaacc ccctggtgcg gtactcaccc gagctgtagg gctgtccgag
    13261 gtacgcgcga ttgctgagcc gcagcgacat gacgccggcc gtcccgtcgt gagtgacgtg
    13321 gtctgcgcgc caggcgttgt cgaagggcga gccattggtc cagcggtcgg ccttgatcca
    13381 gcgctgcgtg tcgtacgtgc cgagctggtc gaggaacgac ccgagcggca cgacggcggg
    13441 cgtgttgccg cgccgggcgg ccagggcctc ggagaacatc gcgacgacca gtccgccagc
    13501 ggccatggcc acgatcgcgc gtgatatgag cgtcgttcga ctgctgaatc ccgtcccctg
    13561 catgagacct cccgacggtt ccggcccggc cagggccggg cacacgcgtc gcttcccctc
    13621 gtcgttctcg ttgtgcgcgc ccccgttcgt ggcggcgtca acgcaggcta ttcgattcgt
    13681 gacgcgcgtc aacctgcgcg tgacataacg tcgcagcgcg tggccgctca gccggcggac
    13741 gtcgccgtgt ggggcttgaa cgagtcttcg ggcgccacgc cgtcgagcgc gtccgctcgc
    13801 gtgatcgccg ccgcgacgcg cggctcggcg agccacggcc gcaggttcca gtcgagccgc
    13861 gcccaccagc cg
//
LOCUS       DASPFR010000129         2206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_282239, whole genome shotgun sequence.
ACCESSION   DASPFR010000129 DASPFR010000000
VERSION     DASPFR010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282239"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VD737_03265"
     CDS             complement(<1..600)
                     /locus_tag="VD737_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09606.1"
                     /translation="MGEINRHGLRDAICSLLAAGALAAAPAMAHNDDVDQPDDTATTS
                     ATSSGTTYGYGAATAADSTTDADQSRSSATTDSSSSTDSTAAGASSPSGTSSPSSASS
                     TSGSTTTDATGPNRADLYSTGQSPDAKSRTGSQSMSTDADQANRTQASNSGAGTRTDV
                     VWYIMPVTLKAQSDQISKGCWVQLFSGDDFEGRYVSVVGP"
     gene            894..1892
                     /locus_tag="VD737_03270"
     CDS             894..1892
                     /locus_tag="VD737_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017245520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXW09607.1"
                     /translation="MLDRPGVVVITGASGGVGRAAAREFARRGADVGLLARNDEALAV
                     ARREVEALGRRACAVPVDVADARSVSNAADVVAGTLGPIDVWVNNAMVTVVSPVAQLD
                     ADELRRVTDVTYHGTVYGTLAALRHMRPRNCGTIVQVGSALAYRSIPLQAAYCGAKAA
                     TRAFTDSLRSELIRDRSGVRVTMVHMPALNTPQFDWCRTRMHRKPQPVAPIFQPEVAA
                     RAIAWAATHRRRELFVGMSTLLAVTGQKLAPGWIDRHLARAGYDGQLSEELVEQGRRD
                     NLWEPLSGDVGAHGRFDDRAHGHSAGLQLSTHRVEVGALLLALLSLGAAAFLRKRP"
     gene            complement(1933..>2206)
                     /locus_tag="VD737_03275"
     CDS             complement(1933..>2206)
                     /locus_tag="VD737_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09608.1"
                     /translation="AYWFDLQAVRSVKLDATAHAYGAAVHALISWQGLHVVLVAIVTA
                     YVLARFRAGLVSARHRVTFDTLRLLWLYTAAQGLVTAVVLHSPRLG"
BASE COUNT          337 a          738 c          748 g          383 t
ORIGIN      
        1 cgggccgacc acggagacgt agcgcccctc gaaatcgtcc cccgaaaaca gctgcaccca
       61 gcagcccttg ctgatctgat cactctgtgc tttcagcgtc accggcatga tgtaccagac
      121 gacgtcggta cgtgtgcccg caccgctgtt cgaagcttga gtgcggttcg cctggtcggc
      181 atccgtgctc attgattgcg agccggtgcg cgacttggcg tcaggcgact ggccggtcga
      241 gtacaggtcc gctcgattcg gcccggtggc gtcggtcgtg gtcgagccgg acgtagagct
      301 cgcactgctc ggcgaactcg tgccgcttgg tgagctcgca cccgccgccg ttgagtctgt
      361 cgatgaactc gaatctgtcg tcgcgctgga gcgcgactgg tcggcatccg tcgtggagtc
      421 agcagccgtg gcggctccgt agccataggt cgtgccgctg ctcgtcgcgg acgtcgtcgc
      481 cgtgtcgtcc ggctggtcca cgtcgtcgtt gtgcgccatc gcgggtgcgg ccgccagtgc
      541 gcccgcggcg agcaagctgc agattgcatc gcgaagtccg tgtctgttga tttcacccat
      601 caagagtctc cttgcttccc agtgaagcgt tttcgttctg gaatcccgat cgcggaaaga
      661 cgatccggct acttctattt cgattcgcca ttacgcgctc ggttccacgg cgcgtccgtc
      721 cgactgcctc ggacattcca gtcagcgatc gcgcacacgc gccgcaccgt tgcgacgatc
      781 cgtgaggaat gcgcaatcag gtacaagcaa gcaagtactg agatgcatca catttgcagt
      841 ccggtcggtt cgacgatcgg ccgcaccgtt tcgctgttgc cgtgagttgc gcgatgctcg
      901 acagaccagg agtcgtggtg attaccggag cgagtggcgg cgtaggccgt gccgcggcgc
      961 gtgaattcgc ccgtcgcggc gcggacgtcg ggctgttggc ccgcaacgac gaagcactgg
     1021 ccgtggcgcg gcgggaggtc gaggctcttg ggcgtcgcgc ctgcgcggtg ccggtcgacg
     1081 tggccgatgc gcgttccgta tccaatgccg ccgatgtcgt cgcgggcacc ctggggccga
     1141 tcgacgtgtg ggtcaacaat gcgatggtca ccgtggtgag cccggtcgcg cagctggatg
     1201 ccgacgaact gcgacgggtg accgacgtga cctaccacgg cacggtgtat ggcacgctgg
     1261 ctgcgctgcg ccacatgcgg ccgcgcaact gcggcacgat cgtgcaggtc ggctcggcgc
     1321 tcgcgtatcg ctcgatccct cttcaggccg cctactgtgg cgcgaaggcg gcgacgcgtg
     1381 ccttcacgga ttcactgcgc tccgaactga tccgcgaccg cagcggtgtc cgcgtgacga
     1441 tggtgcacat gccggcgctc aacacccctc agttcgactg gtgccgcacg cgcatgcacc
     1501 gcaagccgca gccggtcgcg ccgatcttcc agccggaggt ggcggctcga gcgatcgcat
     1561 gggccgcgac gcatcgccga cgcgagcttt tcgtgggcat gtcgacgctg ctcgcggtga
     1621 ccgggcagaa gctggcgcca ggctggatcg accgccacct cgcccgcgcc ggctacgacg
     1681 gccagctctc ggaggaactc gtcgagcagg gtcgccggga caacctctgg gagccgctgt
     1741 ccggcgatgt cggcgcacac ggccgtttcg acgaccgtgc gcacggccac agtgccggac
     1801 tgcagctgtc gacgcacagg gtcgaagtcg gcgcactgct gctcgcgctg ctttcgctcg
     1861 gtgcggccgc cttcctgcgc aagcgccctt gaggccccgc gcgacgcgca tggacgaagg
     1921 gtccgtgcac cgtcacccga gtcgcgggct gtgcagcacg acggccgtca cgagaccctg
     1981 cgccgccgtg tacagccaga gcaggcgcag cgtgtcgaac gtcacgcggt gccgggcgct
     2041 cacgagaccg gcgcgaaacc gcgcgagcac ataggccgtg acgatcgcga ccagcacgac
     2101 gtgcaagccc tgccaggaga tcagcgcgtg cacagcggcg ccgtaggcat gggcggtcgc
     2161 atccagtttc accgagcgca cggcctgcag gtcgaaccag tacgca
//
LOCUS       DASPFR010000130         6133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_282344, whole genome shotgun sequence.
ACCESSION   DASPFR010000130 DASPFR010000000
VERSION     DASPFR010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282344"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VD737_03280"
     CDS             <1..636
                     /locus_tag="VD737_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014998871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HXW09609.1"
                     /translation="DELVFYDITASPQERTVDRAWVSRVARVLDIPFCVAGGIRSVAD
                     AEEVLNAGAEKVSVNSPALSDPPLIDALARRFGSQCIVVGIDSQTVDSGFEVHQYTGD
                     PDRSRGTRRRTLDWVREVQDRGAGEVVLNCMASDGVRRGYDLRQLEAVRAVCQVPLIA
                     SGGAGTPAHFAEVFARANVDGALAASVFHSGAIEIGELKRYLRGENVEMRP"
     gene            633..1262
                     /gene="hisIE"
                     /locus_tag="VD737_03285"
     CDS             633..1262
                     /gene="hisIE"
                     /locus_tag="VD737_03285"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HXW09610.1"
                     /translation="MNVVGLDRLDWAKGGGLLPAIVQDARTGRVLMLGYMNRESLQRT
                     LETRRAVFFSRSRNQLWTKGETSGNFLDVVDVSADCDDDAILVLAHPAGPTCHKGTES
                     CFAQSSLPQASRMAFLATLEATIARRIAESPEGSYTARLYASGLGRIAQKVGEEGVET
                     ALAAVTRDDGALLGECADLLFHILVLLKARNLSFDQVVQELGSRHAARP"
     gene            complement(1263..2582)
                     /gene="creD"
                     /locus_tag="VD737_03290"
     CDS             complement(1263..2582)
                     /gene="creD"
                     /locus_tag="VD737_03290"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HXW09611.1"
                     /translation="MRVSWKILMMFGLTVALLIPLGLIRGTVAERQRYRAEAVEEVAR
                     STAGAQTLAGPILVVPYSDRVTKLRPDESGVQRRVDVVESGTWVFFPRSMAARGRMQS
                     IPRRLGLYDVRVYELDADVRAGFHVTIPRDPDPASPRTLGQPRLAIGLRDVRGLVGVP
                     SLHVDGTPRPLHQGLGPAGGRGLHALLPVPAPGQPIELEVAFKAAVQGTETLSIVPLA
                     ERNSIAIASPWPHPKFHGEFLPRARHVRLDGFDANWEVSSLATNAQAQMREALRQPAS
                     AEVRADSVSVSLVDPVNVYSKVDRATKYGVLFVLLTFVAFFMFELLKQLRIHPIQYGL
                     VGLALSMFFLLLLALSEHVQFAAAYVVAAVACVGLIAYYLGHVLGGYMRAAGFAAMLG
                     TLYAALYGLLVSEDNALVLGAGLLFLVLAAVMVLTRRVDWYQAAARA"
     gene            complement(2666..4159)
                     /gene="creC"
                     /locus_tag="VD737_03295"
     CDS             complement(2666..4159)
                     /gene="creC"
                     /locus_tag="VD737_03295"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF008312.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HXW09612.1"
                     /translation="MRIGLRILLGYFLIVALAAVLLGRVFVQQVKPGVRQAMEDTLVD
                     TANLLAAQARDDLLAGRIADGRFAASVQTLARRDVGAEIWGFDKRRINTRIYVTDARG
                     IVVFDSAGRDVGRDYSQWNDVYLTLRGRYGARSTRADPADESSTVMHVAAPIVDDRAT
                     PPRIVGVLTVAKTNQSIAPFIERSQQTILRWGLVLLGVALIVGLVAAWWLSRRLGALR
                     DYADAATAGERVTLPEAPGEFGDLGRALETMRDRLEGKQYVEHYVHALTHELKSPLAA
                     IRGSVELLESGGAQMPADARERFIATIGAQTDRMAEMVEKLLALAAVEHRQEIENPRP
                     VDVLALAEEAAERVAPKFAQRHVTVRIDGSEATVYGDRFLLRQAIVNLLENAADFAPE
                     GSAVDLAIERRDALCHVIVRDRGPGVPDYALERAFERFYSTPRPGGGSRSSGLGLCFV
                     AEVAALHGGRAGLANRLDGGAEATLVVPVGDHPARRRDESGRWRRPS"
     gene            complement(4172..4867)
                     /gene="creB"
                     /locus_tag="VD737_03300"
     CDS             complement(4172..4867)
                     /gene="creB"
                     /locus_tag="VD737_03300"
                     /inference="COORDINATES: protein motif:HMM:NF008296.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HXW09613.1"
                     /translation="MRATILIAEDESAIAETIAYALRTEGYAVEHVLLGGDVVPRVQA
                     GGVDLVLLDVGLPDVTGFDVCRALRASSDVPVVFLTARGAEVDRIVGLELGADDYVTK
                     PFSPREVVARVRARLRRQARGVEARWEAVDAFEHDREGRRFRYRGQLLDLTRYEYGVL
                     EALLARPGAVLSRTQLMDRVWADALESSDRTVDTHIKTLRAKLRAVSGRGGEGDDPIR
                     THRGVGYSLDPGR"
     gene            complement(5109..>6133)
                     /locus_tag="VD737_03305"
     CDS             complement(5109..>6133)
                     /locus_tag="VD737_03305"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXW09614.1"
                     /translation="VEGVAEATASVLKLVSGRLADRGVRAKSLVVAGYGLSNAMRPLI
                     GLAAAWSAVLVLRFLDRVGKGIRTAPRDALIAGAVPPEQRGRAFGFHRSMDHAGAVVG
                     PLIAFALLSAAVDLGTVFLASVVPGLLVIGLLVLGVPGGERYTAAAPAPTRFSWRGLH
                     ARLRAMIVAAGALALASVPEVFIVLWATQAGMQVKWVPLVWAAASLAKMLIALPAGIV
                     SDRLGRVPVLFGGWTARVAMLGLLAWWPQPSRTVVWLLFIAYSVTLAVTEPAERSLIG
                     DHAAERERGTAYGLYHLASGLLVLPGAVLFGAIWQQAGSSVAFTVAATITAVAAAWLL
                     LLARRH"
BASE COUNT         1001 a         2195 c         2109 g          828 t
ORIGIN      
        1 gacgagctcg tcttctacga catcacggcc agcccgcagg aacgcaccgt cgaccgggcg
       61 tgggtgtcgc gggtcgcgcg cgtgctcgac attcctttct gcgtcgcggg cggcatccgc
      121 agcgtggccg acgccgagga agtgctcaac gcgggcgccg agaaggtctc ggtcaattcc
      181 ccggcgctga gcgacccccc gttgatcgac gcgctggcga gacggttcgg ctcgcagtgc
      241 atcgtggtcg gcatcgacag ccagaccgtg gatagcgggt tcgaggtgca tcagtacacc
      301 ggcgatccgg accgctcgcg tggcacgcga cgccgcacgc tcgactgggt tcgcgaggtg
      361 caggaccgtg gcgccggcga ggtggtgctc aactgcatgg cgagcgacgg ggtgcggcgc
      421 ggctatgatc tgcggcagct cgaggcggtg cgcgcggtct gccaggtgcc gctgatcgct
      481 tcgggcggcg ccggcacgcc ggcccacttc gccgaggtgt tcgcccgggc caacgtagat
      541 ggggcactcg cggccagcgt cttccacagc ggcgcgatcg agatcggcga gctgaagcga
      601 tacctgcggg gggagaacgt ggagatgcgc ccatgaatgt cgtcggcctg gatcgactcg
      661 actgggccaa gggcggcggc ctgctgcccg cgatcgtgca ggacgcgcgc acgggccgcg
      721 tcctgatgct gggctacatg aaccgggagt cgctgcagcg cacgctcgaa acccggcgcg
      781 ccgtgttctt cagccgctca cgcaaccagc tctggaccaa gggcgaaacc tccggaaact
      841 tcctcgacgt ggtcgacgtg agcgccgact gcgacgacga cgcgatcctg gtgctcgcgc
      901 accccgccgg accgacctgc cacaagggca cggagtcgtg cttcgcgcag tcgtccctgc
      961 cgcaggcctc gcgcatggcg ttcctggcca cgctcgaggc gacgatcgcg cgccgcatcg
     1021 cggagtcgcc ggagggcagt tacacggcgc ggttgtacgc gagcggcctc ggccgcatcg
     1081 cgcagaaggt cggcgaggaa ggcgtggaga ccgcgctcgc cgccgtcacc cgcgacgacg
     1141 gcgcgctgct cggcgagtgc gccgaccttc tcttccacat cctggtgctg ctgaaggcgc
     1201 ggaacctgtc gttcgaccag gtggtgcagg agctcggttc gcgtcacgcc gcgcgcccct
     1261 gatcacgccc gcgcggccgc ctggtaccag tcgacccggc gcgtcagcac catgaccgcc
     1321 gcgagcacca ggaacagcag cccggcaccc agcaccagcg cgttgtcttc gctgacgagc
     1381 agcccgtaga gcgccgcgta caacgtgccg agcatcgccg cgaagcctgc cgcgcgcatg
     1441 tatcccccga gcacgtgccc caggtagtag gcgatcagcc cgacgcaggc cacggcggcg
     1501 acgacgtagg ccgccgcgaa ctggacatgt tccgaaagcg cgagcagcag caggaagaac
     1561 atcgacagcg ccagtcctac caggccgtac tggatcggat ggatgcgcaa ctgcttcagc
     1621 agctcgaaca tgaagaacgc gacgaacgtg agcagcacga acagcacgcc gtacttggtc
     1681 gcccgatcga ccttggaata cacgttcacg ggatcgacca gcgagacgct gaccgagtcg
     1741 gcccggacct ccgccgaggc aggctgtcgc agcgcctcgc gcatctgcgc ctgcgcgttg
     1801 gtggcgagcg acgacacttc ccagttcgcg tcgaaaccgt ccagtcggac gtgacgggcc
     1861 cggggcaaga actcgccgtg gaacttcgga tgcggccatg gcgacgcgat cgcgatggag
     1921 ttgcgctcgg cgagcggcac gatcgagagg gtttcggtgc cctgcaccgc cgccttgaac
     1981 gcgacctcga gttcgatcgg ctgccccggc gcaggcacag gcagcagcgc gtgcagcccc
     2041 cggccgccgg ccggacccag cccctggtgc aatgggcggg gcgtcccgtc gacgtgcagc
     2101 gacggcacgc ccacgagtcc gcgtacgtca cgcagtccga tggcgaggcg gggctggccg
     2161 agcgtgcgcg gcgaggctgg atcggggtcg cgtggaatcg tgacgtgaaa gcccgcacga
     2221 acgtcggcgt cgagttcgta cacccgcacg tcgtacaggc ccagccgtcg cgggatcgac
     2281 tgcatgcggc cgcgcgccgc catggagcgc ggaaaaaaca cccacgtccc tgactcgacg
     2341 acgtcgacgc gtcgctgcac cccactctcg tcgggccgga gcttcgtgac acgatcgctg
     2401 tacggcacga cgaggatcgg ccccgccagc gtctgcgcac cggccgtgct gcgcgccacc
     2461 tcctcgacgg cttcggcgcg gtaccgctgg cgctcggcca cggtgccccg gatcaggccc
     2521 agcgggatca gcagggcgac ggtcaggccg aacatcatca ggatcttcca cgacacgcgc
     2581 atgatgcctc tccggctatg tggccgggac accttggacg tgacccgcga aagggcgatg
     2641 agcgacgtgt gaagtgcagg tgaagtcagg acggacgacg ccaccgtccg ctctcgtcgc
     2701 ggcgacgtgc cgggtggtcg ccgaccggca ccaccagcgt cgcctccgcg ccaccgtcga
     2761 gacgattggc cagcccggcc cgcccgccgt gcagcgccgc cacctcggcg acgaagcaga
     2821 gccccaggcc gctgctgcga ctgccccctc ccggtcgggg cgtcgaatag aagcgctcga
     2881 atgcgcgctc gagcgcatag tcgggcacgc ccgggccccg gtcgcggacg atgacgtggc
     2941 agagcgcgtc cctgcgttcg atcgccaagt cgacggcgct gccctcgggc gcgaagtcgg
     3001 ccgcgttctc cagcaggttg acgatggcct gccgcagcag gaagcgatcg ccgtacacgg
     3061 tcgcctccga gccgtcgatg cgcaccgtca cgtggcgttg cgcgaacttc ggtgcgaccc
     3121 gttcggcggc ctcttcggcc agcgccagca cgtcgacagg ccgtgggttt tcgatctcct
     3181 gtcggtgctc gacggccgcg agcgcgagca gcttctcgac catctcggcc atgcgatcgg
     3241 tctgtgcgcc gatcgtcgcg atgaaccgct cacgggcgtc cgcgggcatc tgcgccccgc
     3301 ccgactccag cagttccacg ctgccgcgaa tcgccgcgag gggactcttg agctcgtgcg
     3361 tcaatgcgtg gacgtagtgc tcgacgtact gcttgccctc gagacggtcg cgcatcgtct
     3421 cgagcgcccg tccgaggtcg ccgaactccc ccggtgcctc gggcagcgtg acgcgttcgc
     3481 ccgcggtggc cgcatcggcg taatcgcgca gggccccaag ccggcgcgaa agccaccagg
     3541 ccgccacgag ccctacgatc agtgcgacgc caagcaagac caagccccag cgcaggatcg
     3601 tctgctggct gcgttcgatg aagggcgcga tcgactggtt cgtcttggcg accgtcagca
     3661 cgccgacgat gcgcggaggc gtcgcgcggt cgtcgacgat cggagccgcg acgtgcatga
     3721 ccgtcgacga ctcgtcggcc ggatccgcgc gggtcgagcg cgcaccgtag cgcccgcgca
     3781 gcgtcaggta gacgtcgttc cactgcgaat agtcgcggcc gacgtcgcgg cccgcggaat
     3841 cgaacacgac gatgccgcgg gcatcggtga cgtagatgcg cgtgttgatc cggcgcttgt
     3901 cgaatcccca gatctcggca ccgacgtccc gccgcgcgag cgtctgcacg ctcgctgcaa
     3961 aacgcccatc ggcgatgcgg cccgcgagca ggtcgtcgcg agcctgcgcg gcgagcaggt
     4021 tggccgtgtc gaccagcgtg tcctccatcg cctggcgcac gccgggcttg acctgctgca
     4081 cgaacacgcg gccgagcagg accgccgcca gcgcgacgat caggaagtaa cccagcagga
     4141 ttcgcaggcc gatccgcatg cgtcgccgaa gtcagcggcc cgggtccagc gaatagccga
     4201 cgccgcggtg cgtgcggatt ggatcgtctc cctcaccgcc acgcccgctc accgcccgca
     4261 gcttggcgcg cagcgtcttg atgtgcgtgt cgacggtgcg gtcgctgctc tcgagcgcgt
     4321 cggcccagac ccggtccatc aactgcgttc tcgacagcac ggcgccgggt cgcgcgagca
     4381 gtgcctccag cacgccgtac tcgtaacggg tcaggtcgag cagctgaccg cggtagcgga
     4441 aacgccggcc ctcgcggtcg tgttcgaaag catcgactgc ttcccagcgc gcctcgacgc
     4501 cgcgggcctg ccgccgcagg cgcgcacgca cgcgcgcgac gacttcccgc ggcgagaatg
     4561 gcttggtcac gtaatcgtcc gcgccgagct cgagcccgac gatacggtcg acctcggcgc
     4621 cgcgagcggt caggaacacg accggcacgt cgctggaggc ccgcagcgcc cggcagacgt
     4681 cgaatccggt cacgtctggc aggccgacgt cgagcaggac gaggtccacg ccgccagcct
     4741 ggacgcgcgg cacgacgtcg ccgccgagca gcacgtgctc gacggcgtag ccctccgtgc
     4801 ggagcgcgta cgcgatcgtc tcggcgatgg ccgattcgtc ctccgcgatc aggatggtcg
     4861 cccgcatgcg tcctccggtc accggcgcgc ggacattgta gagcggtgaa ggcatgccat
     4921 cccgggcgtg tagcaggcac ggtacccgcg cgtttcggcc gcccccgagc ccggagtggg
     4981 tcgcaagaca cgccgtaaac ccgtcaaagt tgtcggctct tgttttcacg gccgcccccg
     5041 agcccgggtt cgagcgcggc gttgcagtcg agcgaaggta ttcgcgagcc accgcacccg
     5101 aaacgaaact agtggcgccg cgcgagcaac agcagccacg cggcggcgac ggcggtgatc
     5161 gtcgccgcga ctgtgaacgc gaccgacgag cctgcctgct gccagattgc gccgaacagc
     5221 accgcgcccg gcagcaccag caggccgctg gcgaggtggt aaagaccgta ggccgtaccg
     5281 cgctcgcgtt cggccgcgtg atcgccgatc aacgagcgtt cggcaggttc cgtgacggcc
     5341 aacgtgaccg agtacgcgat gaacagcagc cagacgaccg tccgcgatgg ctgcggccac
     5401 cacgccagca gtccgagcat ggccacgcgt gccgtccagc cgccgaacag taccggaacg
     5461 cgaccgaggc gatctgagac gatccccgcc ggcaacgcga tcagcatctt cgcgaggctc
     5521 gccgcggccc agaccagcgg cacccacttc acctgcatgc cggcctgcgt ggcccacagc
     5581 acgatgaaga cctcgggcac gctcgcaagc gcgagcgcac cggccgcgac gatcatggcg
     5641 cgcaagcgtg cgtgcaaccc ccgccaggaa aagcgggtcg gtgccggcgc ggcagcggtg
     5701 tagcgctcgc ccccaggcac gccgagcacg agcaggccga ttacgagcag ccccggcacg
     5761 acggacgcca ggaacacggt gccgaggtcc actgccgcac tcaagagcgc gaacgcgatc
     5821 agcgggccca cgaccgcgcc ggcatggtcc atcgagcggt gaaagccgaa tgcacggccg
     5881 cgctgctcag gtggtacggc gcccgcgatc agcgcgtcgc gaggcgcggt gcgtatgccc
     5941 ttgccgacgc ggtcgaggaa tcgcaggacg agtacggcgg accacgcggc ggccaggccg
     6001 atcagcggcc gcatcgcgtt cgacaagccg tagcccgcca ccacgagcga cttcgcccgc
     6061 acccctcgat ccgcgaggcg acccgacacc agcttgagca cgctcgccgt ggcttcggcc
     6121 acgccctcga cca
//
LOCUS       DASPFR010000131         2351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_526139, whole genome shotgun sequence.
ACCESSION   DASPFR010000131 DASPFR010000000
VERSION     DASPFR010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526139"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VD737_03310"
     CDS             <1..726
                     /locus_tag="VD737_03310"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXW09615.1"
                     /translation="DFAGCCLDQILNQAAKFRYMFGGKASTPLVIRTMVGADLRAAAQ
                     HSQMLTSLWTHIPGLKVVCPSSPYDAKGLLIQAIRDDDPVMFFEHKALYPRKGEVPEG
                     AYTVPFGEANLLREGEHVTVVAIGRMVVFAEKAIDAVAKDGITCDLIDPRTTSPLDID
                     TILESVDGTGRLVVVDESSPRCSVASDICGMVAQRGFRSLKAAPRMVTAPHAPVPFAR
                     ELERAWVPGPAKIEAAIRDAMRD"
     gene            741..>2351
                     /locus_tag="VD737_03315"
     CDS             741..>2351
                     /locus_tag="VD737_03315"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXW09616.1"
                     /translation="MASKRLFLSVIALAGFVLGAAPVIAADVGEGPKKGAGDAAPRFD
                     QKTTEQAHAGEPLFNQYCAACHLGQVAKAPHKMFLQMMAPDAIYSALDVGIMRAHAEK
                     LSAAQKQQVATYLGGDHPLSTARAPQCTGDAARFDAERRPAASGWGITAANTRHIGAA
                     SAGLTAADLPGLELKWAFAFPNAQRARSQPAVAYGAVYVGSQDGTVYALDERSGCVRW
                     TFRAPAEVRTAVVVESPAAARTRPGPPLVFFGDLLARVYAVDAFTGAVRWSYKADDHP
                     NATITGAPALHGGTLYVPVSSLEVTSAADPAYECCTFRGSVVALDAVTGAPRWKSYTI
                     PTPPKEVGRTRVGTRVLAPSGAPVWNSPAIDARRGLLYVGTGENYSSPANDTSDALLA
                     FRLTDGAMVWHAQKTRNDAWNVACMLPDNPNCPPENGPDVDFGSSVIVFRDPTRGDLL
                     LAGQKSGMVYAVDPEQRGKIVWGSRVGRGGIQGGVHFGMATDGERLYVPISDMKDEHD
                     GRVHPEPSRAGIYALDPRTGRTLWGTPAN"
BASE COUNT          356 a          838 c          816 g          341 t
ORIGIN      
        1 gacttcgccg ggtgctgcct ggaccagatc ctcaaccagg cggcgaagtt tcgctacatg
       61 ttcggcggca aggcctccac gccgctggtg atccgcacca tggtcggcgc cgacttgcgt
      121 gccgccgccc agcattccca aatgctcacc tccttgtgga cgcacattcc ggggctgaaa
      181 gtggtgtgtc cgtcatcgcc ttacgacgcc aagggcctgc tgatccaggc gatccgcgac
      241 gacgacccgg tgatgttctt cgagcacaag gcgctctacc cgcgcaaggg cgaggtgccc
      301 gagggcgctt acacggtgcc gttcggtgag gccaacctgc tgcgcgaagg cgagcacgtc
      361 acggtggtcg cgatcgggcg catggtcgtg ttcgcggaga aggccatcga cgccgtggcg
      421 aaggacggca tcacctgcga cctgatcgac ccgcgcacga cctcgccgct cgacatcgac
      481 acgatcctcg agagtgtcga tggcaccggt cgcctcgtgg tggtcgacga gtcgagtccg
      541 cgctgcagcg tggcctcgga catctgcggc atggtggcgc agcgcggctt ccgctcgctc
      601 aaggccgctc cgcgcatggt cacggcgcca cacgcaccgg tgccgttcgc gcgtgagctg
      661 gagcgtgcct gggtgcccgg ccccgcgaag atcgaggctg cgatccgcga cgcgatgcgc
      721 gactgacccc cgaggtaccc atggcgagca agcgtttgtt cctgtccgtg attgcgctgg
      781 ccgggttcgt gctcggcgcc gcacccgtca tcgcggccga cgtcggcgaa ggcccgaaga
      841 aaggcgccgg ggacgcagcc ccacgcttcg accagaagac gaccgagcag gcgcacgccg
      901 gcgagccgct gttcaaccag tactgcgccg cctgccacct gggccaggtc gccaaggcac
      961 cgcacaagat gttcctgcag atgatggcgc cggacgcgat ctacagcgcg ctcgacgtcg
     1021 gcatcatgcg tgcgcacgcg gagaagctga gcgccgcaca gaagcagcag gtcgcgacct
     1081 acctcggcgg cgatcatccg ctgtcgaccg cccgcgcgcc gcagtgcacc ggcgatgcgg
     1141 ccaggttcga cgccgaacga cggcccgcgg cctcggggtg gggcatcacc gccgccaaca
     1201 cccggcacat cggcgcggcg agcgccggtc tcacggccgc agacctgccg ggcctcgagc
     1261 tcaagtgggc gttcgcgttc cccaatgcgc aacgtgcccg ttcgcagccc gccgtggcct
     1321 acggcgcggt ctacgtgggc agccaggacg gcacggtcta tgcgctcgac gaaaggagcg
     1381 gctgcgtgcg ctggaccttt cgcgcaccgg ccgaagtgcg cacggccgtg gtcgtggaat
     1441 cgccggccgc ggcgcgcacg cgtccagggc cgccgctcgt gttcttcggc gacctgctcg
     1501 cgcgcgtcta cgcggtcgac gcgttcacgg gcgcggtgcg ctggtcgtac aaggcggacg
     1561 accatcccaa cgcgacgatc accggcgcac ccgcgttgca cggcggcacg ctttacgtgc
     1621 cggtctcctc gctcgaggtg acctccgccg cggatcccgc gtacgagtgc tgcacgttcc
     1681 gcggctcggt cgtggccctc gacgccgtga cgggtgcacc gcgctggaag tcgtacacga
     1741 tccccacgcc gccgaaggag gtcggtcgca cgcgcgtcgg cacgcgagtg ctcgcgccgt
     1801 cgggcgcgcc ggtgtggaac tcgcccgcga tcgacgcccg gcgcgggctg ctctacgtcg
     1861 gcaccggcga gaactactcg tcgcccgcca acgacaccag cgacgcgctg ctcgcgtttc
     1921 gcctgaccga cggtgcgatg gtctggcacg cgcagaagac caggaacgac gcgtggaacg
     1981 tggcctgcat gctgcccgac aacccgaact gcccgcccga gaacgggccc gacgtggact
     2041 tcggctccag cgtgatcgtg ttccgcgacc ccacgcgggg cgacctgctg ctcgcagggc
     2101 agaagtcggg catggtctac gcggtggatc ccgagcagcg gggcaagatc gtctggggtt
     2161 cacgggtcgg gcgtggtggc atccagggcg gcgtgcactt cggcatggcg accgacggcg
     2221 agcggctcta cgtgcccatt tccgacatga aggacgagca cgacggtcgc gtgcatccgg
     2281 agccgtcgcg cgccggcatc tacgcgctcg acccgcgcac cgggcgcacg ctgtggggca
     2341 cgcccgccaa c
//
LOCUS       DASPFR010000132         4868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1625340, whole genome shotgun sequence.
ACCESSION   DASPFR010000132 DASPFR010000000
VERSION     DASPFR010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625340"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VD737_03320"
     CDS             complement(<1..425)
                     /locus_tag="VD737_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXW09617.1"
                     /translation="MLEGRALLEMALLPALFPVLLNTPAGDGHPVLLVPGFTAGDSTL
                     IGLSAFLRTRGYHVETWGFGQNTGFKLKFSQALEQKLRYMHHRHKRKVSIVGWSLGGV
                     YAFYAAHAAPECVRSVVSLGSPMRFSVSEYDTPLLVKAI"
     gene            617..2518
                     /gene="pepF"
                     /locus_tag="VD737_03325"
     CDS             617..2518
                     /gene="pepF"
                     /locus_tag="VD737_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HXW09618.1"
                     /translation="MPDQHRTRTFLAMLLLAVVPSVHAVERSEVAEKYRWDLAALYPD
                     EAAWAAAKQQFAKAIPDVASHQGKLGDSAASLLAAMTAYEQAVRQAERLYAYAFQRYS
                     EDTRVGRTMQMQQEMSQAYSDFQAATAFMRPELIAVGREKIDAYVQAEPKLAQYRMFF
                     DDVLRATPHTLGPAEEKVVARMSVLGEAGGTVQSVLRSADLPFPEVTFSDGQKVRLDA
                     AAYEKYRASPNKADRDLAFRSFWDSYNDFKRTFAATLNAHVLSHVAYKDVRKFPTSLD
                     AALFDYNIPRSVYEQLLEDVHANLPTLHRYLKLRRQIMDVEQLGYEDLYAPIVKKVDL
                     KYTPEQAMQMTLDAFAPLGAEYVETLRKGYADRWVDFMPNVGKEGGAYSNTVYGVHPY
                     QFMNFNGAWTDVSTLAHESGHSMHSFMSNAKQPWATSNYSTFVAEVASTLNENLLLHH
                     VLDQTRDDRTRLFLLSSYLDSMRTTLFRQTLFAEFELKIHEMVERGEPLTGESLNELY
                     LGLLRQYYGHDAGVVKVDEAYGAEWAFIPHFYRNFYVYQYATSMIGGMQLADGIIGQQ
                     AKKSGASQKNRDAYLRLMSAGSSKYPVDLLKDAGVDMTTSGPFNAAMREMNTIMDEIE
                     RIYAKGTVK"
     gene            2515..3006
                     /gene="queF"
                     /locus_tag="VD737_03330"
     CDS             2515..3006
                     /gene="queF"
                     /locus_tag="VD737_03330"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HXW09619.1"
                     /translation="MNGARGRRKAPGSIYTGLDQLGRATAQPQSPGEAVLERVPNPHP
                     RKVYLVRFVAPEFTSLCPITGQPDFAHLVVDYAPAKWLLESKSFKLYLASFRNHGAFH
                     EDCTLEVAGRLVDFLQPRWLRIGGYWYPRGGMPIDVFWQSGRPPAGLWIPEQGVPPYR
                     GRG"
     gene            3044..4507
                     /locus_tag="VD737_03335"
     CDS             3044..4507
                     /locus_tag="VD737_03335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXW09620.1"
                     /translation="MTGSLRTTLGAALLTLAAGSSMAAVAPARETPAKRAAVNAVDRH
                     AADLRDLSDRIWAYAETALREHRSAAALADYAEQQGFEVERGVAGMPTAFVATFGSGR
                     PVIGVMGEYDALPGISQKATAVKEPLETGAGGHGCGHNLFGAASLGAAIAIKEQIAAG
                     KLKGTVKFYGTPAEENYGGKIYMAREGLFDGIDAVLAWHPGDETQADMVSSQAMVDLG
                     VEFRGKAAHAASDPWNGRSAVDAVELFTHGVNLMREHVKPTSRMHYTITAGGDVPNVV
                     PEYGRVWLWLRDWKRDEVESLLARTRRLAEGAAAMTETEATVKVLGGSWDMLVNETGE
                     RLLHANLLWLGPATYTDGEQAFAKQIQKATGVPDVGMHPGVNPLENQPQEGGSTDVAD
                     ISWIAPTLHATVATSPKGAPWHAWPVVATAGMSIGHKGMLRAAKVLAATMVDLYEQPQ
                     ALAAVQAEFKAKKGDTVYEAYVPKGPPPVPHDDGKAE"
     gene            4555..4651
                     /gene="ffs"
                     /locus_tag="VD737_03340"
     ncRNA           4555..4651
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VD737_03340"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT          838 a         1641 c         1660 g          729 t
ORIGIN      
        1 tagatcgcct tgaccagcag cggggtgtcg tattcgctaa cggaaaagcg catcgggctg
       61 ccgagcgaga cgacgctgcg cacgcactcg ggcgccgcgt gcgcggcata gaaggcgtat
      121 acgccaccga ggctccaccc gacgatgctg accttgcgct tgtggcgatg gtgcatgtag
      181 cgcagcttct gttcgagcgc ctggctgaac ttgagcttga aaccggtgtt ctggccgaag
      241 ccccaggtct cgacgtgata gccgcgcgtg cgcaggaagg cgctcaggcc gatcagcgtg
      301 gagtctccgg cggtgaagcc gggcaccagc agcaccgggt gaccgtcgcc cgccggcgta
      361 ttcaggagca ccgggaacag cgccggcagc agcgccattt cgagcagcgc ccgcccctcg
      421 agcatcgtcc acaccgccct cggcggcctc gggatccgcg agccgttgga cctggccaat
      481 ttgtgcccct ggttcgacct ggtgttgggt tgcaccgcag catggtagca ggcacgacga
      541 cgttgcggcg accgcaacga ctaggcaagc ttccgcctcc tttttccggg ccgggtgccc
      601 atttccggag ttttcgatgc ccgatcagca ccgtacccgt acgttcctgg cgatgttgct
      661 cctcgccgtc gtcccgtccg tacatgccgt cgaacggtct gaggttgccg aaaagtaccg
      721 ctgggacctc gcggcgctgt acccggacga ggcggcgtgg gcggccgcca agcagcagtt
      781 cgcgaaggcg atcccggacg tcgcctcgca ccagggcaag ctcggcgact ccgccgccag
      841 cctgctcgcc gccatgaccg cgtacgagca ggcggtgcgc caggcggagc gtctctacgc
      901 ctacgcgttc cagcgctaca gcgaggacac gcgcgtcggg cgcaccatgc agatgcagca
      961 ggaaatgtcg caggcatact cggacttcca ggccgcgacc gcgttcatgc gaccggagct
     1021 gatcgccgtc ggccgcgaga aaatcgacgc ctacgtgcag gccgaaccga agctcgcgca
     1081 ataccggatg ttcttcgacg acgtgctgcg ggcgacgccg cacacgctgg gcccggccga
     1141 ggagaaggtc gtggcgcgaa tgtcggtgct cggcgaggcc ggtggcacgg tgcagtcggt
     1201 gctgcgcagc gcagacctgc cgttcccgga agtcacgttc tcggacgggc agaaggttcg
     1261 tctcgacgcg gccgcctacg agaaataccg cgcgtcgccg aacaaggccg accgcgacct
     1321 ggcgttccgg tcgttctggg attcgtacaa cgacttcaag cgcaccttcg ccgcgacgct
     1381 gaacgcgcac gtgctctcgc acgtcgccta caaggacgtg cgcaagttcc cgacctcgct
     1441 cgacgcggcg ctgttcgact acaacatccc gcgcagcgtg tacgaacagc tgctggagga
     1501 cgtgcacgcc aacctgccga cgctgcaccg ctacctgaag ctgcgccgcc agatcatgga
     1561 cgtcgagcaa ctcggctacg aggacctcta cgcgccgatc gtgaagaagg tcgacctgaa
     1621 gtacacgccc gagcaggcca tgcagatgac gctcgacgcg ttcgcgccgc tcggcgccga
     1681 gtacgtcgag acgctgcgca agggctatgc ggatcgctgg gtcgacttca tgcccaacgt
     1741 cggcaaggaa ggcggcgcct acagcaacac cgtgtatggc gtgcacccgt accagttcat
     1801 gaacttcaac ggcgcgtgga ccgacgtctc gacgctcgcg cacgagtccg gccactcgat
     1861 gcactcgttc atgtcgaacg cgaagcagcc gtgggcgacg tcgaactatt cgacgttcgt
     1921 ggcggaggtg gcctcgacgc tcaacgagaa cctgctgctg caccacgtgc tcgaccagac
     1981 ccgggacgac cgcacgcgac tgttcctgct gtcgagctac ctcgacagca tgcgtacgac
     2041 gctgttccgc cagacgctgt tcgccgagtt cgaactcaag atccacgaga tggtcgagcg
     2101 cggcgagccg ctgacgggtg aatcgctcaa cgagctgtac ctcggcctgc tgcggcagta
     2161 ctacggtcac gacgccggcg tcgtgaaggt cgacgaggcc tacggcgcgg agtgggcctt
     2221 catcccgcac ttctaccgca acttctacgt gtaccagtac gccaccagca tgatcggcgg
     2281 catgcagctc gccgacggca tcatcggcca gcaggcgaag aagtccgggg cctcgcagaa
     2341 gaaccgcgac gcgtacctgc ggctgatgtc cgcgggctcg tccaagtacc ccgtcgacct
     2401 gctcaaggac gcgggcgtcg acatgacgac gtccgggccc ttcaacgccg cgatgcgcga
     2461 gatgaacacg atcatggacg agatcgagcg catctacgcg aagggcacgg tcaagtgaac
     2521 ggtgcccgcg ggcgccgcaa ggcgcccggt tcgatctaca cgggcctcga ccagctcggc
     2581 cgcgcgactg cgcagccgca gtcgccgggc gaggccgtgc tggagcgcgt gccgaaccca
     2641 cacccgcgca aggtgtatct cgtgcgcttc gtggcgcccg agttcacgtc gttgtgcccg
     2701 atcacggggc aaccggattt cgcgcacctc gtcgtcgact acgccccggc gaagtggctg
     2761 ctcgagagca agtcgttcaa gctctacctc gcgagcttcc gcaaccacgg tgccttccac
     2821 gaggactgta cgctggaggt cgccggtcgc ctggtcgact tcctgcagcc gcgctggctg
     2881 cgcatcggcg gttactggta tccgcgcggc gggatgccga tcgacgtgtt ctggcagtcc
     2941 ggtcgcccgc cggcgggatt gtggatcccg gagcagggcg tgccgccgta tcgcggccgc
     3001 ggatgacgac gagaagacca gcctgcccat gaggagccat cgcttgacgg gatcactgcg
     3061 cacgacgttg ggtgccgcgt tgctgacgct ggccgcgggt tcgtccatgg ccgccgttgc
     3121 tcctgcacgc gaaacgcctg ccaagcgggc ggccgtcaac gccgtggatc gccatgcggc
     3181 ggacctgcgg gatctctccg accggatctg ggcctacgcc gagacggcgc tgcgcgagca
     3241 caggtcggcc gccgcgctgg ccgactacgc cgagcagcag gggttcgagg tcgagcgcgg
     3301 cgtggccggc atgccgacgg cgttcgtcgc gaccttcggg tcgggacgtc ccgtcatcgg
     3361 cgtgatgggg gagtacgacg cgctgcccgg catttcgcag aaggcgacgg cggtcaagga
     3421 gccgctcgag acgggcgcgg gaggtcacgg ctgcggccac aacctgttcg gcgccgcgag
     3481 cctcggcgct gcgatcgcga tcaaggagca gatcgccgcc ggcaagctca aaggcaccgt
     3541 gaagttctac ggcacgccgg cggaggagaa ctacggcggc aagatctaca tggcgcgcga
     3601 aggtttgttc gacggcatcg acgcggtgct ggcgtggcat cccggtgacg agacccaggc
     3661 cgacatggtc tccagccagg cgatggtcga cctgggcgtc gagtttcgcg gcaaggcggc
     3721 gcacgccgcg agcgacccgt ggaacggccg cagcgcggtc gatgcggtcg agctgttcac
     3781 gcacggcgtc aacctgatgc gcgaacacgt gaagcccacc tcgcgcatgc actacacgat
     3841 caccgcgggc ggcgacgtgc cgaacgtcgt gccggaatac gggcgggtct ggctctggct
     3901 gcgcgactgg aagcgcgacg aggtcgagag cctgctcgcg aggacgcgca ggctcgccga
     3961 gggcgcggcg gcgatgaccg aaaccgaggc tacggtgaag gtactgggcg gctcgtggga
     4021 catgctcgtc aacgagaccg gcgagcgcct gctgcatgcg aacctgctgt ggctcggccc
     4081 ggccacgtac accgacggcg agcaggcgtt cgcgaagcag atccagaagg cgaccggggt
     4141 tcccgacgtg gggatgcacc cgggcgtcaa tccgctcgag aaccagccgc aggaaggcgg
     4201 ctcgacggac gtcgcggaca tcagctggat tgccccgacg ctgcacgcga ccgtggcgac
     4261 gtcgcccaag ggggcgccct ggcacgcgtg gcccgtcgtg gcgaccgcgg gcatgtcgat
     4321 cgggcacaag ggcatgctgc gcgccgcgaa agtgctggcc gcgacgatgg tcgacctcta
     4381 cgagcagccg caggcgctcg ccgcggtgca ggctgaattc aaggcgaaga agggcgatac
     4441 ggtctacgag gcctacgtgc cgaaagggcc accgcccgtg ccgcacgacg acggcaaggc
     4501 cgaatgaggg ctcctcgacg cgtgatatag ttgccggcct ccaatggtgg ctcgcgtcgg
     4561 atccctcgcg acggccagcc gtaaaccccg ccaggcccgg aagggagcaa cggtagcggc
     4621 aacgccgggt gccgggggta ttctggcgcg ggccgccgcc tacctgcccg cctcgacgcc
     4681 attccaccca agcgctgctg accgccgatg agctacctcg tccttgcccg caagtggcgc
     4741 ccgcgcacgt tcgaccagct cgtcggccag gatcacgtcc gccgcgcgct cacgaacgcg
     4801 ctcgactccg gccgcatcca ccacgcgttc ctgttcacgg gtacgcgagg cgtgggcaag
     4861 acgaccat
//
LOCUS       DASPFR010000133         6369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_770980, whole genome shotgun sequence.
ACCESSION   DASPFR010000133 DASPFR010000000
VERSION     DASPFR010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770980"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VD737_03345"
     CDS             <1..731
                     /locus_tag="VD737_03345"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HXW09621.1"
                     /translation="GLAAHHASQDPDRFASTLDWAVKLTIIVVLPATLALLLLAGPLT
                     VAIFHYGRFDEHDVHMSTLALMAYSLGLLGFSMVKVLAPGFFARQDTRTPVRIGIQSL
                     VLSMALNVAVVLPLAFIYPGRPGLHALLALNTGLGAWFNATMLYRGLRKQHVLHHSPG
                     WARTLWQVLAGNVAMGLFLWLVAGDTQRWIEMDAWRRVGWMAVLVVGGAGIYFGTLYV
                     LGMRLGHLRVQRVTVPPRPVDLPP"
     gene            728..1723
                     /gene="ribF"
                     /locus_tag="VD737_03350"
     CDS             728..1723
                     /gene="ribF"
                     /locus_tag="VD737_03350"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004163.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HXW09622.1"
                     /translation="MKLRIRCAWENISWLREIEFVRGLHNLRESHRGCVATIGNFDGV
                     HRGHQHMISAVRKRARELDLPATLITFEPTPREYFLGLKAPARLMRLREKLEALALYG
                     IERVVVLRFDERMRNMGAREFESFLLAEKLGVRHMVIGDDFAYGRNREGVAATLRAAG
                     ERHGFTVERVSSFLVNGERVSSSLVREALGEGDLDRAATLLGRPYRMTGRVRQGARLG
                     RTLGYPTANLGLHRKVVPLWGILAVRVSCAGMADHPAIVSLGTRPTVNGTEPLLEVHL
                     FDYCGDLYGQYLSVDFVRWLRAERKFDSLDALVEQMNCDARQARAALTKSEVHPC"
     gene            1810..4638
                     /gene="ileS"
                     /locus_tag="VD737_03355"
     CDS             1810..4638
                     /gene="ileS"
                     /locus_tag="VD737_03355"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HXW09623.1"
                     /translation="MADYRNTVNLPETAFPMKADLARREPDMLAWWEQHRTYEKLRDH
                     ARGRPAFVLHDGPPYANGAIHIGHAVNKILKDIIVKSRSLDGYDAPYVPGWDCHGLPI
                     EHAVEKVRGRDAKNLDAKAFRQACREFALEQIDVQRRDFIRLGVMGDWSNPYMTLAPG
                     YEAEQLRAFAQIIRNGHLYKGFKPVHWCLDCRSALAEAEVEYEERTSPSIDVKFGAVD
                     ARDLARRFAIDVQLPASSSVLIWTTTPWTLPANQAVAVHAEFDYSLVKTVRGDGGPEE
                     VLVLASELVSAVLTRAGLQGAQELGRVKGAALEGLQLQHPFYDRVVPVILAPHVTLES
                     GTGAVHTAPGHGQEDFVIGKRYGLAVDNPVGNDGRFLPTTPLFAGEKVFDANKHVVAV
                     IAERGALLHHEAFRHSYPHCWRHKTPVIFRATPQWFISMEQAGLRKAALAEIGRVEWT
                     PDWGEQRIYSMIEGRPDWCISRQRTWGVPLTLFTHKETGALHPRTPELVDEVATLVEG
                     AGIDAWFDLDPAALLGDEAASYEKVTDIMDVWFDSGVSHHCVSKLFPEVRTPADLYLE
                     GSDQHRGWFHSSLLTSVAQKGHAPYKGVLTHGFTVDEKGRKMSKSLGNVILPQKVMST
                     LGADVLRLWVAATDYANEIAVSDEILKRTSDSYRRIRNTVRFLLGNMAGFDPARDAVP
                     TERMVALDRWAIARTAALQQEVADAYRSYDFHLIYQKVHNFCVVDLGGFYLDVIKDRL
                     YTTPAGGHPRRSAQTALYWIAEAMVRWLAPILSFTAEEIWRFMPGERGESVFFETWAA
                     LPAIDPNDLGVDWERVLAARAAVLRELERLRVAEQIGAPLDAAVTLYGTEEWTRALAP
                     LGEELRFVLITSEAIVADAAERPSDAAPADEEAKTGLWLQVRPVGHAKCARCWHKRPD
                     VGTVAAHPELCARCVVNLDGPGESRRYA"
     gene            4679..5278
                     /gene="lspA"
                     /locus_tag="VD737_03360"
     CDS             4679..5278
                     /gene="lspA"
                     /locus_tag="VD737_03360"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019486629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HXW09624.1"
                     /translation="MSTTATPPTPPPPQHEHRRRASDQPGHPLHHASVAERAALLVQR
                     THPAAWLWLSLAVVALDQVTKFLVTRFLELYERVEVLPVLDFTLLHNTGAAFSILADA
                     SGWQRGFFIVLGIVVCLVLIVWLWRLPRGERLLPISLALIVGGAVGNVIDRVAHGYVI
                     DFIHAHWGEAYFPAFNVADSAITIGAALLLLDAFREQRN"
     gene            5389..6357
                     /gene="ispH"
                     /locus_tag="VD737_03365"
                     /gene_synonym="lytB"
     CDS             5389..6357
                     /gene="ispH"
                     /locus_tag="VD737_03365"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017387043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HXW09625.1"
                     /translation="MKILLANPRGFCAGVDRAIDIVERALELFGAPIYVRHEVVHNRF
                     VVDRLRELGALFVEELDEVPDGATVIFSAHGVSKAVQAEARRRGLRVFDATCPLVTKV
                     HMEVSRYAREGLDVVLIGHEGHPEVEGTMGQFDEANGGRIMLVEDASDVDQLAVRDPA
                     RVAVVTQTTLSVDDTADVLDALKRRFPILATPRKEDICYATQNRQDAVKKLVADCDVV
                     VVVGSVTSSNSNRLREIAQKAGKPGYLVDGPDDLRPEWFAGKSAVGVTAGASAPELLV
                     LRVVERLRQWGGEGAEEIVGRAENVVFALPRELRQAAGTPRDDVSA"
BASE COUNT          974 a         2163 c         2204 g         1028 t
ORIGIN      
        1 caggcctcgc ggcccatcat gcctcgcagg accccgaccg cttcgcctcg acgctcgact
       61 gggcggtgaa gctcacgatc atcgtggtgt tgccggcgac gctcgcgctg ctgctgctcg
      121 ccgggccgct gacggtggcg atcttccact acgggcgctt cgacgagcac gacgtgcaca
      181 tgtccacgct cgcgctgatg gcctattcgc tcgggctgct cggcttcagc atggtcaagg
      241 tgctcgcccc ggggttcttc gcgcgccagg acacgcgcac ccccgtcagg atcggcatcc
      301 agtcgctcgt cctcagcatg gcgctcaacg tcgccgtggt gctgccgctc gcgttcatct
      361 atccgggcag gccgggcctg catgcgctgc tcgcgctcaa caccggcctc ggcgcctggt
      421 tcaacgccac gatgctctat cggggtttgc gcaagcagca cgtcctgcac cattcgccgg
      481 gctgggcgcg cacgctgtgg caggtgctcg ccggcaacgt ggcgatggga ctcttcctct
      541 ggctcgtggc gggcgacacg cagcgctgga tcgagatgga cgcctggcgc cgcgtcggct
      601 ggatggccgt gctggtggtg ggcggcgccg gcatctattt cggaacgctg tacgtgctcg
      661 gcatgcggct cgggcacctg cgcgtccagc gtgtcaccgt accgccgcgg ccggtggacc
      721 tgccgccgtg aagctcagga tccgctgcgc ctgggagaac atctcgtggc tgcgcgagat
      781 cgagttcgtg cgcgggctgc acaacctgcg cgagtcgcac cgcggctgcg tggccacgat
      841 cggcaatttc gacggcgtgc accgcggcca ccagcacatg atctcggccg tccgcaagcg
      901 cgcgcgcgag ctcgacctgc cggcgaccct gatcacgttc gagccgacgc cgcgcgaata
      961 cttcctgggc ctcaaggcac ccgcgcgact gatgcgactg cgcgagaagc tcgaggcgct
     1021 ggcgctctac ggcatcgagc gcgtcgtcgt gctgcgcttc gacgagcgga tgcgcaacat
     1081 gggcgcgcgc gagttcgaga gcttcctgct cgcggagaag ctcggcgtgc gtcacatggt
     1141 gatcggggac gacttcgcct acggccgcaa ccgcgagggc gtcgccgcga cgctgcgcgc
     1201 cgcgggcgag cgccacgggt tcaccgtcga acgggtgtcc tctttcctgg tcaacggcga
     1261 gcgcgtcagc agctcgctcg tgcgcgaggc gctcggcgag ggtgacctcg atcgcgccgc
     1321 cacgctgctc ggacgaccgt atcggatgac ggggcgtgtc cggcaaggcg cgcggctggg
     1381 ccggacgctc ggctatccga ccgccaacct cggactgcac cgcaaggtcg ttccgctgtg
     1441 gggcatcctg gccgtgcgcg tcagctgcgc cggcatggcg gaccaccccg cgatcgtcag
     1501 cctcggcacg cgcccgacgg tgaacggcac cgagccgctg ctcgaggtgc acctgttcga
     1561 ctactgcggt gacctctacg gccagtacct gagcgtggac ttcgttcgct ggctgcgggc
     1621 cgaacgcaag ttcgactccc tcgatgcgct ggtcgaacag atgaattgcg atgcgcgaca
     1681 ggcgcgcgcg gcgctgacca agtccgaagt gcatccgtgc tgagccgcgg ctcggctgga
     1741 tcagggcgct gaattcattc acaatttcgg gttccgtgcc cgcacccctt ccaggccgcc
     1801 cgacccgcca tggctgatta caggaacacc gtgaacctgc cggagacggc cttcccgatg
     1861 aaggccgacc tcgcgcggcg cgagccggac atgctcgcct ggtgggagca gcatcgcacg
     1921 tacgagaagc tgcgcgatca cgccaggggc cgcccagcgt tcgtgctgca cgacgggccg
     1981 ccgtatgcca acggcgccat ccacattggc cacgccgtca acaagatcct caaggacatc
     2041 atcgtcaagt cgcgctcgct ggacggctac gacgcgccgt acgtgcccgg ctgggactgt
     2101 cacggcctgc cgatcgaaca cgccgtcgag aaagtgcgcg ggcgcgacgc caagaacctc
     2161 gacgccaagg cgttccgcca ggcctgccgc gagttcgcgc tggaacagat cgacgtgcag
     2221 cgccgcgact tcatccgtct cggcgtgatg ggcgactggt ccaacccata catgacgctc
     2281 gcgcccggct acgaggcgga gcagttgcgc gcattcgcgc agatcatccg caacggccac
     2341 ctgtacaagg gcttcaagcc cgtgcactgg tgcctggact gccgctcggc gctggccgag
     2401 gccgaggtcg agtacgagga gcgcacctcg ccgtcgatcg acgtcaagtt cggcgccgtc
     2461 gatgcgcgcg acctcgcgcg ccggttcgcg atcgatgttc aactgccggc gtcgtccagc
     2521 gtcctgatct ggacgaccac gccgtggacc ctgccggcca accaggcggt cgcggttcac
     2581 gcggagttcg actactcgct cgtcaagacc gtgcgcggtg acggcgggcc ggaggaggtg
     2641 ctcgtactgg cgagcgagct cgtgtccgcg gtgctgacgc gtgctggctt gcagggcgcg
     2701 caggaactcg gacgagtgaa gggcgcggcg ctcgagggcc tgcaactgca gcacccgttc
     2761 tacgaccgcg tcgtgccggt gatcctggcc ccgcacgtca cgctcgagag cggcaccggc
     2821 gccgtgcata cggcgcccgg ccacggccag gaagacttcg tcatcggtaa gcgttacggg
     2881 ctcgcggtcg acaacccggt cggcaacgac gggcgcttcc tgccgacgac gccgctgttc
     2941 gccggcgaga aagtgttcga cgccaacaag cacgtcgtgg ccgtgatcgc ggaacgtggc
     3001 gcgttgctgc accacgaggc gttccggcac agctatccac actgctggcg tcacaagacg
     3061 ccggtgatct tccgcgcgac cccgcagtgg ttcatctcga tggagcaggc gggactgcgc
     3121 aaggctgcgc tcgcagagat tggtcgcgtc gagtggacgc cggactgggg cgagcagcgc
     3181 atctacagca tgatcgaagg gcgccccgac tggtgcatct cgcggcagcg tacctggggc
     3241 gtgcccctga cgctgttcac gcacaaggag accggagcgc tgcaccctcg cacaccggag
     3301 ctcgtcgacg aggtggcgac gctcgtcgag ggcgccggca tcgacgcctg gttcgacctc
     3361 gacccggccg cgctgctggg cgacgaggcc gcgagctacg agaaggtcac cgacatcatg
     3421 gacgtctggt tcgactccgg ggtctcgcat cattgcgtct cgaagctgtt ccccgaggta
     3481 cgcacgcccg ccgacctcta cctcgagggc tcggaccagc atcgtggctg gttccacagt
     3541 tcgctgctca cgtccgtcgc gcagaaaggc cacgcgccct acaagggcgt gctcacccac
     3601 gggttcaccg tggacgagaa gggccgcaag atgtcgaagt cgctcggcaa cgtgatcctg
     3661 ccccagaagg tcatgagcac gctcggcgcc gacgtgctgc ggctctgggt cgcggccacc
     3721 gactacgcca acgagatcgc cgtttccgac gagatcctga aacgcacgtc ggattcgtac
     3781 cggcgtatcc gcaacaccgt gcgcttcctg ctcggcaaca tggcggggtt cgaccccgcg
     3841 cgcgacgcgg taccgaccga gcggatggtc gcgctcgatc gctgggccat cgcccggacg
     3901 gccgcgttgc agcaggaagt cgccgatgcc tatcgcagct acgacttcca cctgatctac
     3961 cagaaggtgc acaacttctg cgtcgtggac ctcggcggct tctacctcga cgtcatcaag
     4021 gaccgtctct acacgacgcc ggccggcggt cacccgcgcc gctcggcgca gacggcgctc
     4081 tattggattg ccgaggcgat ggtgcgatgg ctcgcgccga tcctgtcctt cacggccgag
     4141 gagatctggc gcttcatgcc gggcgagcgg ggcgagtcgg tgttcttcga gacttgggcc
     4201 gcgctgcccg cgatcgaccc gaacgacctc ggggttgact gggagcgcgt gctcgcggca
     4261 cgcgcggccg tgctacggga actcgagcgc ctccgcgtgg ccgagcagat cggcgcgccg
     4321 ctcgacgctg ccgtgacgct ctacggcacc gaggagtgga cccgagcgct cgcaccgctc
     4381 ggtgaggagc tgcggttcgt gctgatcacc tcggaggcga tcgtcgccga cgcggccgag
     4441 cgtccgtcgg acgccgcgcc tgcagacgag gaggccaaga ccgggctgtg gctgcaggtg
     4501 cggcccgtgg gacacgcgaa gtgcgcccgt tgctggcaca agcggcccga tgtcggtacc
     4561 gtggcggcgc acccggagtt gtgcgcgcgc tgcgtggtca acctagacgg gcccggcgaa
     4621 tcgcgccgct acgcctgagc cgtacgggcg gcaccaagcc tctggacgac atctacgcat
     4681 gagcacgacc gcgactccgc cgacgccgcc accgccgcag cacgagcacc ggcgtcgcgc
     4741 gtcggatcag cccggccacc cgctgcacca cgccagcgtg gccgagcgtg cggcgctgct
     4801 ggtccagcgg acccatccgg ccgcgtggct gtggctgtcg ctggcggtcg tggcgctgga
     4861 tcaggtcacg aagttcctgg tcacgcgctt cctggagctc tacgagcgcg tcgaagtgct
     4921 gccggtgctc gacttcacgc tgctgcacaa caccggggcg gcgttcagca tcctcgcgga
     4981 cgcgtcgggg tggcaaaggg gctttttcat cgtgctcggc atcgtcgtgt gcctggtgct
     5041 gatcgtctgg ctctggcgcc tgccgcgcgg ggagcgcctg ctgccgatct cgcttgcgct
     5101 gatcgtgggc ggtgccgtcg gcaacgtgat cgaccgcgtc gcgcacggct acgtgatcga
     5161 cttcatccac gcgcactggg gcgaggccta ttttccggcg ttcaacgtcg ccgacagcgc
     5221 gatcacgatc ggcgcggcgc tgctgctgct cgatgcgttc agggaacagc gaaactagtg
     5281 gctagtggtc agtggctagt ggctagtgat gagaagtaca tttaacgtgc cgacgtacct
     5341 tcttgatccg acactaacca ctaaccacta gccactagcc acttcttaat gaagattctc
     5401 ctcgccaatc cccgcggttt ctgtgcgggt gtcgaccgcg ccatcgacat cgtcgagcgg
     5461 gccctcgagc tgttcggcgc gccgatctac gtgcgccacg aggtggtgca caaccgcttc
     5521 gtggtcgatc ggctgcggga gctgggcgcg ctcttcgtcg aggagctcga cgaggtgccg
     5581 gacggcgcca ccgtgatctt cagcgcgcac ggcgtctcga aggcggtgca ggccgaggct
     5641 cgccgccgcg ggctgcgcgt gttcgacgcg acctgtccgc tggtaaccaa ggtgcacatg
     5701 gaagtctcgc gctatgcgcg cgaaggcctc gacgtcgtcc tgatcggcca cgagggccat
     5761 ccggaagtcg aaggcacgat ggggcagttc gacgaggcga acggcgggcg catcatgctg
     5821 gtcgaggacg cttccgacgt cgaccagctc gcggtgcgcg accccgcgcg ggtcgccgtg
     5881 gtcactcaga ccacgctttc ggtcgacgat acggcggacg tgctcgacgc gctcaagcga
     5941 cgttttccga tactcgcgac gccgcgcaag gaagacatct gctacgcgac gcagaaccgg
     6001 caggacgcgg tcaagaagct ggtcgccgac tgcgacgtcg tggtcgtggt gggctcggtt
     6061 acgagctcga attcgaaccg gctgcgcgag atcgcgcaga aggcgggcaa gccggggtac
     6121 ctcgtcgacg gtccggacga cctgcggccc gagtggttcg cgggcaagag tgcggtcgga
     6181 gtcacggccg gagcctcggc gccggagctg ctggtgctac gcgtcgtcga gcgactgcgc
     6241 cagtggggcg gggagggcgc cgaggagatc gtgggacgcg cggagaacgt cgtgttcgcg
     6301 ctgccgcggg aactgcggca ggcggcggga acgccacgcg acgacgtgtc ggcctgaggg
     6361 ccgggcacg
//
LOCUS       DASPFR010000134         9321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_526380, whole genome shotgun sequence.
ACCESSION   DASPFR010000134 DASPFR010000000
VERSION     DASPFR010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9321
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526380"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /gene="dapE"
                     /locus_tag="VD737_03370"
     CDS             <1..1122
                     /gene="dapE"
                     /locus_tag="VD737_03370"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HXW09626.1"
                     /translation="LALTEDLLRRPSVSPEDYGCLDVIAGRLAALGFAVERLDFGPVA
                     NLWARRGEAAPVLCFAGHTDVVPSGPREEWATDPFEPVIVDGVLYGRGAADMKSGLAA
                     MVTALERYVTRAPQHRGSLAVLFTSDEEGPSVDGTKKVVEALEARGEKIDWCVVGEPT
                     SVEALGDTIKVGRRGSLSGRLTVHGVQGHIAYPHLADNPVHRFAPALAELVATRWDEG
                     NRYFQPTSFQVSNIAAGTGAPNVIPGELKVRTNLRFSTEQTVATLQQRVLEILDRHGL
                     NYTMDWHISGLPFLTPPGELTDAVARAVRETTGRVPEYSTTGGTSDGRFIAPTGAQVV
                     EFGVVNATIHKVNECVRVADIETLSRAYERVMELLLGEK"
     gene            complement(1291..2097)
                     /locus_tag="VD737_03375"
     CDS             complement(1291..2097)
                     /locus_tag="VD737_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09627.1"
                     /translation="MTQEEKVSQAQQDLQLKVWKELAISKQILMRAAAEALKLDPECT
                     QDELKEALEGALKKIAKADADLFNAKEEAKVAIASLEKKLAASEHALAISQKEAAEAK
                     TAHDGVVQQMANQRAASSMELQKVKDRLSEQEKALKAINTALADTPANVLKKMNALKK
                     QKQEEANARREIESALNTLRSEKRQQDQKLADLQRHGESLVGRYRELHEVSSKLQEQL
                     RPLVEDAKSLPEVPPLDTKLLEAIEQPADANAKRPARNNERLNDRPNARS"
     gene            complement(2231..2752)
                     /locus_tag="VD737_03380"
     CDS             complement(2231..2752)
                     /locus_tag="VD737_03380"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HXW09628.1"
                     /translation="MNRSLDTRAGVPTRRNANLLGFLWCAFLVAYALYAQYVQLQEPC
                     PLCILQRVAVIAVGVVFLAAVIHDPADRGARVYGVLIDLLALAGIGVAARHLWILSQP
                     PGSVAECGASLDYMLDVLPLHEVLTKVLTGSGECAKVTWEFLGISMPGWVLIHLVGLA
                     LLGSVANFVLRRK"
     gene            complement(2805..3554)
                     /gene="phoU"
                     /locus_tag="VD737_03385"
     CDS             complement(2805..3554)
                     /gene="phoU"
                     /locus_tag="VD737_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019694308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HXW09629.1"
                     /translation="MAMDTSGLSHHIHSRYNAELEGVRSGVLQMGGLVEQQVQNGVKA
                     VVSGDSRLGEDVARLDPRVNAMEVAIDEDCSRILATRSPTASDLRLIVAVIKTITDLE
                     RIGDEAEKLGHAAARFAAGERPADRYRELKHIGELVAEMLHGALDAFARLDTEAAIAV
                     ARRDRAIDEEYDAIQRQCITFMMEDPRAIRRTLDIMAVARALERIGDHAKNLCEYVVY
                     MVLGKDIRHLSIEDVEKHVLAARAGRPAGKT"
     gene            complement(3643..5007)
                     /locus_tag="VD737_03390"
     CDS             complement(3643..5007)
                     /locus_tag="VD737_03390"
                     /inference="COORDINATES: protein motif:HMM:NF028240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HXW09630.1"
                     /translation="MNKTVLATAVAAALTLGAGQASAQSKGTATKADVQAIEARMQAL
                     AERLEKLEATNAQLQSDNAELKSLADRREAEVDYLKAQTRELREEGAVASNDLAKVKG
                     TDWASKIKLRGDLRYRHEYIGTERVVDDGVEDAADRNRQRIRARFGIDAKVTDNVKGT
                     LAFATGGDDPRSSNQTLGGVSTRKSIGLDMAFVDWAFMPGGNAVLGKQANPIFRPGQS
                     LFYDGDFNPEGLAVKLDRGMFFGTAYGWWLTEQFNSDPDGTNADSRIVGLQAGVKFPL
                     FGGETVLAANYYECGLCQDQSPIYANNANGNTTYRVGTSTTNLLTYDYDVVELAAQVG
                     TMVGSLPLTFWANYAQNMADDVEYDTAYGAGVVLGKASNARTWEAGAFYQSIDKDAVY
                     AQWLDSDFGNGNTDADGWVLKAGYAPVRNFTVNATYFLNTLNKDVGTELDYDRLQLDL
                     NYKF"
     gene            complement(5243..6595)
                     /gene="phoR"
                     /locus_tag="VD737_03395"
     CDS             complement(5243..6595)
                     /gene="phoR"
                     /locus_tag="VD737_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HXW09631.1"
                     /translation="MARRLPTPALSRAAWRYVSFLAGGLLIALLVGAIFGHAAAFAAL
                     YLAAALAWQVTQLVRFERWLRLRTVLKPPNLGGLWGDAVDVTHRLYQRKRFHKRRVLT
                     LLRELRRMTSAMPDGAVLLGPAREILWFNRTAAQWLGLRRKLDYGLRIDNLIRQPEFV
                     DYVRSGGEGVAPRIHMPDQGDRWFVFNLVTTSEWALQLMIVRDVTAEARLEHMRRDFV
                     ANASHELRSPLTVVSGYLDTLADDPTLDEAWREPVREMQRQSERMKNIVQDLLDLSRL
                     EAGSGEAERTPVEVGGLLALIRKEALARPKPPAEFALRLDSDALLLGAEPELHSIFQN
                     LVSNAVKYTPADGRIAVRYWTDGAGAHVSVQDTGIGIAAAHIPRLTERFYRVDAGRSR
                     KLGGSGLGLAIVKHALQRHGGQLEVQSVEGQGSTFTCHFPAERVQARVGTVESALHPS
                     "
     gene            complement(6599..7294)
                     /gene="phoB"
                     /locus_tag="VD737_03400"
     CDS             complement(6599..7294)
                     /gene="phoB"
                     /locus_tag="VD737_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HXW09632.1"
                     /translation="MTGKQILIVEDEKPIRDMIAFGLRRAGYTVREAEDCGEARSSIA
                     DSRPDLLLVDWMLPDQSGLELTRFLKRNKDTQDLPVIMLTARAEEHDKVAGLEGGADD
                     YVTKPFSPRELLARVQAVLRRTSPAAADGTLEAGALALDIASHRVSAGDRTVPLGPTE
                     YRLLQFLMEHPERVYTRSQLLDRIWGGNVYVEERTVDVHIRRLRKALEPFGCDALIQT
                     VRGAGYRLSTRPE"
     gene            complement(7342..8076)
                     /gene="tsaB"
                     /locus_tag="VD737_03405"
     CDS             complement(7342..8076)
                     /gene="tsaB"
                     /locus_tag="VD737_03405"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HXW09633.1"
                     /translation="MSGVSPALHVLAIDTSTELCSAAVVTRDGRSILRSALTERSHAD
                     LILPMVDEVLAEAGLRLRDLDGLAFGRGPGGFTGLRIAAGVVQGLAYGAGLKVAPVSS
                     LAAVAFLASHAEAGEVAEGILVCNDARMNELYWACYRFDAADPLRAHGLTAERVSPPD
                     RVEPAPGVRHFAGNGIARHPQLRERLEAAGLRFHDGLYPRADAIARLGVAMLGRGEGV
                     DAMQALPTYVRDDVARPAAGPVTGMS"
     gene            complement(8073..>9321)
                     /locus_tag="VD737_03410"
     CDS             complement(8073..>9321)
                     /locus_tag="VD737_03410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HXW09634.1"
                     /translation="VAAQFLGYSVSTRQLANLSKDVAGELLLAQQMAGSVDAALSGLD
                     GQIAAVLSVTAGQESRLEHGQWPERLLESLNGLGARTRELADAIAPLGGPDGQAAFER
                     LHERLLEASERLRELTAEEAPGGVRWAETSARNVSAHFAPVDVAQQLSALLQAQPCAW
                     VLTSATLAVGDDFSHYKRRSGLARAETVRFESPFDFPSQALMYLPKGLGDPGAPGHSR
                     DVIRAALPVIEASGGRAFLLFTSHRGLREGAEELYRHWGEQPPYPVLIQGGASRDQLL
                     RAFREAGNAVLLGTGSFWEGIDVKGEALSVVVIDKLPFAAPDDPLLKARLAAIREQGG
                     NPFFDEQVPQAVIALKQGVGRLIRDESDFGVVMLCDTRLVTKGYGRSFITSLPPMRRT
                     RSLDEVQAFLRERLGTTSPVAVT"
BASE COUNT         1341 a         3231 c         3235 g         1514 t
ORIGIN      
        1 ctcgccctga ccgaagacct gctgcgccgc ccctccgtga gccccgagga ctacggctgc
       61 ctggacgtga tcgccgggcg cctcgcggcg ctgggattcg ccgtcgagcg gctcgacttt
      121 gggccggtgg cgaacctctg ggcgcgtcgt ggcgaggccg cccccgtact ttgcttcgcc
      181 ggacacaccg acgtggtgcc gagcggcccg cgcgaagagt gggcgaccga tccgttcgag
      241 ccagtcatcg tcgacggcgt tctctacggg cggggcgccg cggacatgaa gagcggcctc
      301 gccgccatgg tcacggcgct ggaacggtac gtcacgcgtg cgccacagca ccgcggatca
      361 ctggcggtgc tgttcacgag cgacgaggaa ggtccttcgg tcgacggcac gaagaaggtc
      421 gtcgaagcgc tggaagcccg gggcgagaag atcgactggt gcgtggtcgg cgaacccacg
      481 agcgttgaag cgctcgggga cacgatcaag gtcgggcggc gcggatcact gtcaggacgt
      541 ctcacggtgc acggcgtgca ggggcacatc gcctaccctc acctcgccga caacccggtg
      601 caccggttcg cgccggcgct ggccgaactc gtcgcaaccc gctgggacga gggcaaccgt
      661 tatttccagc cgacgtcgtt ccaggtctcg aacatcgcgg ccggtacggg cgcgccgaac
      721 gtgatccccg gcgaactgaa agtccgcacc aacctgcggt tctcgaccga gcagacggtc
      781 gcgacgctgc agcagcgcgt gctcgagatc ctcgaccggc acggcctcaa ctacacgatg
      841 gactggcaca tttcgggcct gccgttcctg acgccgccgg gcgagctgac cgacgccgtc
      901 gcacgcgcgg tgcgcgagac gacgggtcgc gtgcccgagt actcgacgac cggcggcacg
      961 tccgacggcc gcttcatcgc gccgaccggc gcgcaggtcg tcgagttcgg ggtcgtcaac
     1021 gcgacgatcc acaaggtcaa cgagtgcgtg cgcgtcgccg acatcgagac gctgagcagg
     1081 gcgtacgagc gagtcatgga gctgctgctc ggcgaaaagt gactggtggc tgagtgaata
     1141 gtgactcgtc ggagtagacc gcgtcatcgc cgttcgctcc cagatggcgc ggcgccggcc
     1201 ccaaaatgaa acggggcgag gggtgatccc tcgcccacgt tctcaggccg ggcgctggcg
     1261 cgctgccgac ccctctaaca gaccgccgcg tcaggaacgc gcgttgggac ggtcgttcag
     1321 acgctcgttg ttgcgggccg ggcgcttggc gttggcatcc gccggctgct cgatggcctc
     1381 cagcagcttc gtgtcgagcg gcggaacctc cggcaggctc ttcgcgtcct cgacgagcgg
     1441 gcgcagctgc tcctgcagct tcgatgacac ttcgtgcagt tcgcgatagc ggcccaccag
     1501 gctctcgcca tgacgctgca ggtcggccag cttctggtcc tgctggcgct tttcgcttcg
     1561 cagcgtattc agcgcggact cgatctcgcg acgcgcgttc gcttcttcct gcttctgctt
     1621 cttgagcgcg ttcatcttct tgaggacgtt ggcgggcgtg tcggccagcg ccgtgttgat
     1681 cgccttgagc gccttttcct gctcgctgag gcgatccttg accttctgca gttccatcga
     1741 ggaggcagcg cgctgattgg ccatctgctg gaccacaccg tcgtgggcgg tcttggcctc
     1801 ggcggcttcc ttctgggaaa tggccagggc atgctcgctc gcagccagct tcttctcgag
     1861 cgacgcgatg gcgaccttcg cctcttcctt cgcgttgaag aggtcggcgt cggccttggc
     1921 gatcttcttc agggcgcctt ccagcgcttc cttcagctcg tcctgggtgc actcggggtc
     1981 gagcttgagc gcctcggcgg ctgcccgcat caggatctgc ttgctgatgg cgagctcttt
     2041 ccagactttc aactgaaggt cttgttgcgc ttgactcact ttttcctctt gggtcatggg
     2101 atgggtcgat cggaccgcca attgtattcc acgcccgggc cgccggcgac tcgtaggatc
     2161 cgaatccgat ggtttatgac ccggaggcga tgcccccagg gccgccctgc ccggcccgct
     2221 ttgggcggcg tcacttcctc ctgagcacga agttcgccac gctgcccagc agcgccagcc
     2281 ccaccaggtg gatcagcacc caccccggca tgctgatgcc gaggaattcc caggtgacct
     2341 tggcgcactc accggagccg gtcagcacct tcgtgaggac ttcgtgcagc ggcagcacgt
     2401 ccagcatgta gtcgaggctc gcgccgcact cggcgaccga accgggaggc tgcgagagta
     2461 tccacaggtg gcgtgcggcc acgccgatgc cggcgagcgc cagcaggtcg atcaggacgc
     2521 cgtagacgcg cgcgccgcgg tcggctgggt cgtggatcac ggcggcgagg aacaccacgc
     2581 cgaccgcaat caccgcgacc cgctgcagga tgcagagcgg acacggctcc tggagctgga
     2641 cgtactgcgc gtagagcgcg tacgccacca ggaacgcgca ccacaggaag ccgagcaggt
     2701 tggcgttgcg acgggtcggc acgccggcgc gcgtatcgag cgaacggttc atcgaaactc
     2761 caagggggca tccggccgtc gcgcgtcgtc ccgccggcgg tgcatcaggt cttgcccgcc
     2821 ggtcgcccgg cacgggccgc gagcacgtgt ttctcgacgt cctcgatgga caggtgccgg
     2881 atgtccttgc ccagcaccat gtagacgacg tactcgcaga ggttcttggc gtggtcgccg
     2941 atgcgctcga gcgcgcgcgc gacggccatg atgtcgagcg tgcggcggat cgcacgcggg
     3001 tcttccatca tgaaggtgat gcactgccgc tggatggcgt cgtactcctc gtcgatggcg
     3061 cggtcgcgcc gggcgaccgc gatggccgcc tccgtgtcca gccgggcgaa ggcgtcgagg
     3121 gcgccgtgga gcatctccgc cacgagctcg ccgatgtgct tgagctcccg atagcggtcg
     3181 gcgggacgtt caccggccgc gaaccgcgcc gccgcgtggc cgagcttctc ggcctcgtcg
     3241 ccgatgcgct ccaggtcggt gatggtcttg atcacggcca cgatcagccg caggtccgag
     3301 gcggtcgggc tgcgcgtggc gaggatccgg ctgcagtcct cgtcgatcgc gacctccatc
     3361 gcgttgacgc gcgggtcgag ccgggcgacg tcctcgccga gacgcgaatc gccgctcacc
     3421 acggccttca cgccgttctg aacctgctgc tcgacgagcc cgcccatctg cagcacgccc
     3481 gagcgcacgc cctccagttc cgcgttgtag cggctgtgga tgtggtgcga aaggcccgaa
     3541 gtgtccatcg ccatgtgtgt ctcccggagg cggcgttttt acagcacggc ccgggtcgcg
     3601 gcaagcgcga accgggccgt ggggatcgcg gagaaccgcg gctcagaact tgtagttcag
     3661 gtccagctgc agccggtcgt agtcgagctc cgtcccgacg tccttgttga gcgtgttcag
     3721 gaagtacgtc gcgttgaccg tgaagttcct gacgggcgcg tacccggcct tcaggaccca
     3781 gccatcggcg tcggtgttgc cgttgccgaa gtccgagtcg agccactggg cgtacacggc
     3841 atccttgtcg atcgactggt agaacgcgcc ggcttcccag gtccgggcgt tgctggcctt
     3901 gccgagcacg acgccggcgc cgtacgcggt gtcgtactcc acgtcgtccg ccatgttctg
     3961 ggcgtagttg gcccagaacg tgagcggcag cgagccgacc atggtgccca cctgcgccgc
     4021 cagctcgacc acgtcgtagt cataggtcag caggttggtg gtgctggtgc cgacccggta
     4081 cgtcgtgttg ccgttggcgt tgttcgcata gatgggcgac tggtcctggc agagtccgca
     4141 ctcgtagtag ttggccgcga gcaccgtctc gccaccgaac agcgggaact tgacccccgc
     4201 ctgcaggccg acgatgcgcg agtcggcgtt cgtgccgtcg ggatcggagt tgaactgctc
     4261 ggtgagccac cagccgtacg ccgtgccgaa gaacatgcct cggtcgagct tgaccgccag
     4321 gccttcgggg ttgaagtccc cgtcgtagaa cagcgactgg cccgggcgga agatcgggtt
     4381 ggcctgcttg ccgagcacgg cgttgccgcc cggcatgaac gcccagtcga cgaacgccat
     4441 gtcgagcccg atcgacttgc gcgtcgagac gccgcccagg gtctggttcg acgaacgcgg
     4501 atcgtcaccg ccggtcgcga acgcgagcgt gcccttgacg ttgtcggtga ccttcgcgtc
     4561 gatgccgaag cgcgcacgga tgcgctggcg gttccggtcg gccgcgtcct ccacgccgtc
     4621 gtcgaccacg cgttcggtgc cgatgtattc gtggcgatag cgcaggtcgc cgcgcagctt
     4681 gatcttcgag gcccagtccg tgcccttgac cttggcgagg tcgttcgagg cgacggcgcc
     4741 ctcctcgcgc agctcgcgcg tctgcgcctt gaggtagtcg acctcggcct cgcgccggtc
     4801 cgccagtgac ttgagttcgg cgttgtcgct ctgcagctgc gcgttggtcg cctcgagctt
     4861 ctcgaggcgc tcggcgagcg cctgcatgcg cgcctcgatc gcctggacgt ccgccttcgt
     4921 ggcggtgcct ttggattgcg cggacgcctg cccggcgccg agcgtcagcg cggcggcgac
     4981 ggccgtcgca agcacagtct tgttcacggt agaggtcccc ttctgggtgt gggtcgaaac
     5041 acgtcgaccg gcgccggacc tgccggccgg ccggcctcgg cgcacgttgt acgcgccgca
     5101 tgtgacaacc agattgcggt tcgacgacaa ccgtgcgaca gcggccgtgt gctgccgcgc
     5161 cgtggcgttc atcgagtgga aataatgacg ttccggtgcg aggccgggcg gccggtgacg
     5221 tcgaatcggg ccgcgcgagc cgtcaggacg gatggagcgc gctttcgacc gtgccgacgc
     5281 gtgcctgtac gcgctccgcg ggaaagtggc aggtgaacgt gctgccctgg ccttcgacgc
     5341 tctgcacctc gagctggccg ccgtggcgct gcagggcgtg cttgacgatc gcgagcccaa
     5401 ggcccgagcc gccgagcttg cgcgatcgcc ccgcgtcgac gcgatagaag cgctcggtga
     5461 ggcgcgggat gtgcgctgcg gcgatgccga tcccggtgtc ctgcaccgac acgtgcgcgc
     5521 cggcgccgtc cgtccagtag cgcacggcga tgcgcccgtc ggcgggcgtg tacttgacgg
     5581 cgttgctgac gaggttctgg aagatcgaat gcagttcggg ctcggcgccg agcagcagcg
     5641 cgtccgagtc cagccgcagc gcgaattcgg ccggcggctt tggtcgcgcg agcgcttcct
     5701 tgcggatcag cgcgagcagc ccgcccactt cgacgggcgt ccgttcggcc tcgccgctgc
     5761 ccgcctcgag ccgcgagagg tcgagcaggt cctgcacgat gttcttcatg cgctcggatt
     5821 gccgctgcat ctcgcgcacc ggctcgcgcc acgcctcgtc gagcgtcggg tcatcggcga
     5881 gcgtgtcgag gtagccactc acgacggtga gcggcgagcg caactcgtgc gaggcgttcg
     5941 ccacgaaatc gcggcgcatg tgctcgaggc gcgcctcggc ggtgacgtcg cgcacgatca
     6001 tcagctgcag cgcccactcg ctggtggtga cgaggttgaa cacgaaccag cgatcgccct
     6061 ggtcgggcat gtggatgcgt ggggcgacgc cctcgccgcc gctgcgcacg taatcgacga
     6121 actccggctg acggatcagg ttgtcgatcc ggaggccgta gtcgagcttg cgccgcaggc
     6181 ccagccactg ggcggccgtg cggttgaacc agaggatttc ccgggccggc ccgagcagga
     6241 cggcgccgtc cggcatggcc gacgtcatgc gccgcagctc acgcagcagc gtcagcaccc
     6301 ggcgcttgtg gaaccgcttg cgctgataga ggcgatgcgt gacgtcgacg gcgtcacccc
     6361 agagcccacc caggttcggc ggcttgagta cggtgcgcag ccgcagccag cgctcgaacc
     6421 gcacgagctg ggttacctgc cacgccagcg ccgcggccag gtagagcgcg gcgaacgcgg
     6481 cggcgtgacc gaagatcgcg ccgaccagca gcgcgatgag caggccgccc gccaggaacg
     6541 acacgtaccg ccaggccgct ctcgacagcg caggcgtcgg taaccggcgc gccactcgtc
     6601 actccggccg cgtcgaaagg cggtaaccgg ccccgcgcac ggtctggatc agcgcgtcgc
     6661 atccgaacgg ctcgagcgcc ttgcgcagcc ggcggatgtg cacgtccacg gtgcgttcct
     6721 ccacgtacac gttgccgccc cagatgcggt cgagcagctg gctgcgcgtg tagacgcgct
     6781 ccgggtgctc catcaggaac tgcagcagcc ggtactcggt cgggccgagc ggcaccgtgc
     6841 ggtcgccggc cgacacgcgg tggctcgcga tgtcgagcgc gagcgcgccg gcctcgagtg
     6901 tcccgtcggc ggcggccggc gaggtgcggc gcagcaccgc ctgcacgcgt gcgagcagct
     6961 cgcgcgggct gaacggcttg gtcacgtaat catcggcgcc gccctcgagc ccggcgacct
     7021 tgtcgtgctc ctccgcgcgc gcggtcagca tgatcaccgg caggtcctgc gtgtccttgt
     7081 tgcgcttcag gaagcgcgtg agttcgagcc cgctctggtc cggcagcatc cagtcgacga
     7141 gcagcaggtc gggtcgcgaa tcggcgatgc tcgaacgcgc ctcgccgcag tcctcggcct
     7201 cgcgcacggt gtatcccgcg cgacgcagcc cgaacgcgat catgtcgcgg atcggctttt
     7261 cgtcctcgac gatcaggatc tgcttgcccg tcatggtgtc tgccgccggc gtgagcattg
     7321 caggcgcatt acagacgttg attatgacat tcccgtgaca ggccccgcgg ccgggcgggc
     7381 gacgtcgtca cgcacgtacg tcggcagcgc ctgcatcgcg tcgacgccct cgccgcgccc
     7441 gagcatcgcg acgccgagcc gcgcgatcgc gtcggcacgc ggatacaggc cgtcgtggaa
     7501 gcgcagcccg gccgcctcga gccgctcccg cagctgcgga tggcgggcga tgccgttgcc
     7561 ggcgaagtgc cggacgccgg gcgccggctc gacgcgatcc ggcggactga cgcgctcagc
     7621 ggtgagcccg tgtgcgcgca ggggatccgc cgcgtcgaag cggtagcacg cccagtagag
     7681 ttcgttcatg cgcgcgtcgt tgcagacgag gatgccctcc gccacctctc ccgcttcggc
     7741 gtgagaggcc aggaacgcga cggctgcgag gctcgagacc ggcgccactt tcaaacccgc
     7801 gccgtaggcg agaccttgca cgacgcccgc cgcgatgcgc agtcccgtga agccgcccgg
     7861 gccgcgaccg aacgcgaggc catcgaggtc ccgcagccgc agccctgctt ccgcgagcac
     7921 ctcgtcgacc atcggcagga tcaggtccgc gtgactgcgc tcggtcagcg ccgatcgcag
     7981 gatcgagcgg ccgtcgcgcg tcacgacggc ggccgagcag agctcggtcg aggtgtcgat
     8041 cgcgagcacg tgcagcgccg gcgacactcc gctcacgtga ccgccaccgg gctcgtcgtg
     8101 ccgagccgtt cgcgcaggaa tgcctgcacc tcgtcgagcg agcgcgtgcg tcgcatcggc
     8161 ggcaggctcg tgatgaacga gcggccgtag cccttggtca cgaggcgcgt gtcgcacagc
     8221 atgacgacgc cgaagtcgct ctcgtcgcgg atcagccgcc ccacgccctg cttgagcgcg
     8281 atcaccgcct gcggcacctg ctcgtcgaag aaagggttgc cgccctgctc gcggatcgcc
     8341 gcgagccgcg ccttgagcag cgggtcgtcc ggcgccgcga acggcagctt gtcgatgacc
     8401 accacgctca gcgcttcgcc cttgacgtcg atgccttccc agaaactgcc ggtgccgagc
     8461 agcacggcgt tgccggcctc gcggaacgcg cgcagcagct ggtcgcgcga ggcgccgccc
     8521 tggatcagca ccgggtacgg cggctgctcg ccccagtgcc ggtagagttc ctcggcgcct
     8581 tcacgcaagc cgcggtggct cgtgaagagc aggaacgcgc gaccgccgct cgcctcgatc
     8641 acgggcaggg ccgcgcgaat cacgtcgcgc gagtgccccg gcgcgccagg gtcgcccagg
     8701 cctttcggca ggtacatgag cgcctggctc gggaagtcga agggactctc gaaccgaacc
     8761 gtttcggcac gcgcgaggcc gctgcggcgc ttgtagtgcg agaagtcgtc gccgaccgcc
     8821 agcgtcgcgg aggtgagcac ccacgcgcag ggctgcgcct gcagcagcgc ggagagctgc
     8881 tgcgccacgt cgaccggcgc gaagtgcgcg ctgacgttgc gcgcgctcgt ctcggcccac
     8941 cgcacgccgc cgggcgcctc ctccgcggtc aattcccgca atcgctccga cgcttcgagc
     9001 agacgttcgt gcagacgctc gaacgcggcc tgaccgtcgg gcccgccgag cggcgcgatc
     9061 gcgtcggcca gctcgcgcgt gcgggcgccg agcccgttca gcgattcgag caggcgctcg
     9121 ggccactgcc cgtgttcgag acgcgactcc tggcccgccg tgaccgacag caccgcggcg
     9181 atctgcccgt cgagcccgct cagcgccgcg tcgaccgaac ccgccatctg ctgcgccagc
     9241 agcagttcgc cggccacgtc cttcgacagg ttcgcgagct gccgcgtgct gacgctgtag
     9301 cccaggaact gcgcggcgac g
//
LOCUS       DASPFR010000135         3324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_38555, whole genome shotgun sequence.
ACCESSION   DASPFR010000135 DASPFR010000000
VERSION     DASPFR010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38555"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /gene="bioD"
                     /locus_tag="VD737_03415"
     CDS             <1..471
                     /gene="bioD"
                     /locus_tag="VD737_03415"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HXW09635.1"
                     /translation="DAGTSLDLPRLVSWLRSAAPAAQLRLVEGVGGWRVPLHPSGYTS
                     DLPEALALDVVLVVGLTLGCLNHARLTVEAIERGGRCRLAGWIASGVDAAYERSDDNV
                     ISLADVIGAPPVARIPHLRAAPRSPFGNPVHDFDDDAGALLDVLGITPQPRRPG"
     gene            599..1117
                     /locus_tag="VD737_03420"
     CDS             599..1117
                     /locus_tag="VD737_03420"
                     /inference="COORDINATES: protein motif:HMM:NF021517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HXW09636.1"
                     /translation="MVVSTVEAGSSACEHRIELAPNCSLTPQSARIFVASVAVLTLGT
                     ALVFALQGLWPVLPFAGVEVGVLWWAVRSSLRSGRQREAIVISDGAIVVERRGPACSS
                     RLVFPRHWATVKLRDPPVALHPRRLTIESHGRACEVGRFLTEDERHALAVRLRRLVGR
                     TSESPALETRLD"
     gene            1191..2135
                     /gene="coxB"
                     /locus_tag="VD737_03425"
     CDS             1191..2135
                     /gene="coxB"
                     /locus_tag="VD737_03425"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HXW09637.1"
                     /translation="MPKNMLKRVVAAPGAVGLALLLGPGPALADWTLNLRTGVTELSR
                     EIYGLHMLILWVCVAIAVAVFSVMIYSIATFRKSKGAVPATFDHSTGAEVTWTLIPVV
                     ILVAMAIPAARTLVKIEDASGSEMTVKVTGYQWKWQYDYVDEGVSFFSTLAADHNVAR
                     QLDSGVNVADVDHYLLDVDNPLVVPVGRKVRVLVTAADVLHAWWVPDFGLKKDAIPGY
                     INELWFKADKVGTYRGQCAELCGRDHAFMPVVVRVVEQAEYDAWLASQKSARQAATPA
                     AAPATGPAAPDVPAADEAAAVTDTHRSLPQTVLVADAE"
     gene            2142..>3324
                     /locus_tag="VD737_03430"
     CDS             2142..>3324
                     /locus_tag="VD737_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HXW09638.1"
                     /translation="MSQTNQTHPDVLSRTYHDVEVSAQGHHDDHGPARGIMRWLTTTN
                     HKDIGTMYLVFSLVMFLVGGAMALVVRAELFMPGMQLVDPQFFNTMTTVHALIMVFGA
                     VMPATVGLANWMIPLQIGAPDMALPRMNNFSFWLLVAAFSLLILSLFMPGGGPASGWT
                     LYPPLVLQTGDALPLAIFAIHLAGASSIMGAINIIVTIMNLRAPGMSLLKMPMFVWAW
                     LITAYLLIAVMPVFAGAVTMLLTDHFFGTTFFAASGGGDPVLYQHIFWFFGHPEVYIM
                     ILPAFGIVSEIIPTFARKRLFGYDAMVYATASIAFLSFIVWAHHMFTVGMPLAGEMFF
                     MLSTMLIAVPTGVKVFNWTATMWKGSLSFETPMLFALAFLFLFTIGGFSGLMMAVAPI
                     DF"
BASE COUNT          467 a         1129 c         1138 g          590 t
ORIGIN      
        1 gacgccggca cgtcgctcga cctgccccga ctggtctcct ggttgcgcag cgcggcgccc
       61 gctgcgcagt tgcggctcgt cgagggcgtc ggcggctggc gcgtgccgct gcaccccagc
      121 gggtacacga gcgacctgcc ggaggccctc gcgctcgacg tggtcctggt ggtggggctg
      181 acgctgggat gcctgaacca cgcgcgcctc acggtggagg cgatcgaacg cggcgggcgc
      241 tgccggctgg cgggatggat cgccagcggc gtcgacgctg cctacgagcg cagcgacgac
      301 aatgtaatca gcctggcaga cgtgatcggc gccccgccgg tggcgcggat cccgcatctg
      361 cgcgccgcgc cgcgctcgcc gttcggcaat ccggtgcatg acttcgacga cgacgcgggg
      421 gcgctgctcg acgtcctcgg catcacgccg cagccccgtc gacccggcta gcgaggcgca
      481 agccgtcgtc cgacgacggg tcaggcgcgg gctgcgctgc ggcgctccat cacgcgttgt
      541 cgaggtcgtg tacgggtggg tacatttgtt caattccgat cagtccgggt gcaccaggat
      601 ggtcgtcagc acggtcgaag caggctcgag cgcctgcgag caccgcatcg agctcgctcc
      661 gaactgctcg ctgacccccc agtcggcgcg catcttcgtc gcgagcgtcg ccgtgctgac
      721 gctcggcacc gcgctcgtgt tcgcgttgca gggactgtgg cccgtgctgc cgtttgccgg
      781 ggtcgaggtg ggcgtgctgt ggtgggccgt gcgttccagc ctgcgctccg gacgccaacg
      841 ggaagcgatc gtgatcagcg acggggcgat cgtcgtcgag cgtcgtggcc cggcctgttc
      901 gagccggctg gtatttcctc gccattgggc gacggttaaa ctgcgcgacc cacccgtggc
      961 cctgcatccg cgccgcctca cgatcgagtc acacggccga gcctgcgaag tgggtcgctt
     1021 cctcaccgag gacgagcggc acgcactggc agtgcgtctg cgccgactgg tgggcaggac
     1081 gagcgaatct ccggcgctgg agacacggtt ggattgaccg gtctccgcgc aggtcagcag
     1141 tcgccccacg cacgagggcg gcgccggttt cacgacggga gtcttgggta gtgccaaaga
     1201 acatgctgaa gcgggtcgtt gcggccccgg gcgcggtcgg cctcgcgctg ctgctgggcc
     1261 cgggcccggc cctggcggac tggacgctca acctgcgcac gggggtgacc gagctgagcc
     1321 gcgagatcta cggcctgcac atgctgatcc tgtgggtctg tgtcgccatc gcggtcgccg
     1381 tgttcagcgt gatgatctac tcgatcgcca ccttccgtaa gtcgaagggc gccgtgcccg
     1441 cgacgttcga tcacagcacc ggggcggagg tcacctggac gctgatcccg gtggtcatcc
     1501 tggtcgccat ggcgattccg gcggcgcgca cgctcgtcaa gatcgaggat gcgagcggtt
     1561 ccgaaatgac cgtcaaggtc accggctatc agtggaagtg gcagtacgac tacgtcgacg
     1621 agggcgtgtc gttcttttcg acgctcgcag cggatcacaa cgtcgcgcgc cagctcgatt
     1681 ccggcgtgaa cgtggcggac gtcgaccact acctgctcga cgtggacaac ccgctcgtcg
     1741 tgccggtcgg ccgcaaggtg cgcgtgctcg tgacggccgc cgacgtgctg cacgcgtggt
     1801 gggtcccgga tttcggcctc aagaaggacg cgatccccgg ctacatcaac gagctctggt
     1861 tcaaggccga caaggtcggc acgtaccggg gacagtgcgc cgagctctgc ggccgcgacc
     1921 acgcgttcat gcccgtggtg gtgcgcgtgg tcgagcaggc cgagtacgac gcgtggctcg
     1981 cctcgcagaa gtccgcgcgt caggccgcca cgccggccgc ggcacccgcc accgggccgg
     2041 ccgccccgga cgtgcctgca gccgacgagg ctgccgcggt caccgacacc catcgatcgc
     2101 tgccgcagac cgtgctggtc gcggacgcgg agtaaccgtc catgagccag accaaccaga
     2161 cccatcccga cgtcctgagc cgcacgtatc acgacgtcga ggtctccgcg caggggcacc
     2221 acgacgacca cgggccggcg cgcggcatca tgcgctggct caccacgacc aaccacaagg
     2281 acatcgggac catgtacctg gtgttcagcc tggtcatgtt cctggtgggc ggcgcgatgg
     2341 cgctggtcgt gcgcgccgag ctgttcatgc cgggcatgca gctggtggac ccgcagttct
     2401 tcaacaccat gaccacggtg cacgcactga tcatggtgtt cggcgcggtc atgcccgcga
     2461 cggtcggcct cgcgaactgg atgatcccgc tgcagatcgg cgcgcccgac atggcgctgc
     2521 cgcgcatgaa caacttcagc ttctggctgc tggtcgcggc gttctcgctg ctgatcctgt
     2581 cgctgttcat gccgggcggc ggtcccgcgt cgggctggac gctctatcct ccgctggtgc
     2641 tgcagaccgg cgacgcgctg ccgctcgcga tcttcgcgat ccacctggcg ggcgcctcgt
     2701 cgatcatggg cgcgatcaac atcatcgtga cgatcatgaa cctgcgcgcg ccgggcatgt
     2761 cgctgctcaa gatgccgatg ttcgtgtggg cctggctgat caccgcctac ctgctgatcg
     2821 cggtgatgcc ggtgttcgcg ggcgcggtga cgatgctgct gaccgaccac ttcttcggca
     2881 cgacgttctt cgcggcctcg ggcggcggcg acccggtgct gtaccagcac atcttctggt
     2941 tcttcggaca ccccgaggtc tacatcatga tcctgccggc gttcgggatc gtgtccgaga
     3001 tcatccccac cttcgcgcgc aagcggctgt tcggctacga cgcgatggtc tacgcgacgg
     3061 cctcgatcgc gttcctgtcg ttcatcgtct gggcccatca catgttcacg gtcggcatgc
     3121 cgctcgcggg cgagatgttc ttcatgctgt cgacgatgct gatcgccgtg ccgaccggcg
     3181 tgaaggtctt caactggacc gcgacgatgt ggaagggctc gctctcgttc gagacaccca
     3241 tgctgttcgc gctcgcgttc ctgttcctgt tcacgatcgg cggcttctcc gggctgatga
     3301 tggccgtcgc gccgatcgac ttcc
//
LOCUS       DASPFR010000136         4460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1259854, whole genome shotgun sequence.
ACCESSION   DASPFR010000136 DASPFR010000000
VERSION     DASPFR010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259854"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VD737_03435"
     CDS             <1..536
                     /locus_tag="VD737_03435"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HXW09639.1"
                     /translation="RRTAWYYNAWDSRAEGMATAMEEIVLHAGYYDDNPRAREIVWIM
                     LAQRAARGLASLYAHANLMDIRQAKAFQVEWTPRGWMRPDLDLLHFEQQLYLRQPGYG
                     TSYVTGKYLIDELVKDRSHQQGAAFTLGGFIDEFNAAGMMPVSLIRWQLTGDASMVPG
                     CAAAGARCEPASATTRP"
     gene            complement(537..953)
                     /locus_tag="VD737_03440"
     CDS             complement(537..953)
                     /locus_tag="VD737_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HXW09640.1"
                     /translation="MPTDESPRRDDERLSVQRVTLNDERVAGLGALADSLGYRLFDAD
                     LAGCHDKAGLLERLGRAMAFPNWFGGNWDAWFDCLTDLGWHAPAPGYVLVMRRALGMH
                     RSAPETLDTALAIVEDAARVWADRGVTLRAFVDLEA"
     gene            complement(956..1357)
                     /locus_tag="VD737_03445"
     CDS             complement(956..1357)
                     /locus_tag="VD737_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HXW09641.1"
                     /translation="MSRPWLFVLLAAVGLVAANGWFAHERPSAVVEAPQAPPDASEPT
                     GLPPEAIATLQAIADGGPFRYDRDGTVFQNREGLLPQQPRGYYREYTVETPGSRDRGA
                     RRIVTGGDPPEVYWYTEDHYRSFRRIEAAPP"
     gene            complement(1361..2155)
                     /locus_tag="VD737_03450"
     CDS             complement(1361..2155)
                     /locus_tag="VD737_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXW09642.1"
                     /translation="MAGVFRDGLFEGRTAFVAGGTSGINLGIAERFADLGAAVTVVSR
                     SPEKVDTAVRHLQARGARAFGQACDVREYDAIEAALSAAAAHFGPIDMLVSGAAGNFV
                     ARALDMSAKGFRTVVDIDLLGTFNVLRAAHAHLRKPGAAIVNISAPQAYMPMPGQSHV
                     GAAKAGVDMLTRNLAVEWGRDGIRVVSIVPGPIEDTEGMRRLGAAPGAREKLERSIPL
                     GRYGRKGEIADLAAFLCSDAAQYVTGAVVVCDGGQSLLGSHGFLPD"
     gene            2311..4332
                     /locus_tag="VD737_03455"
     CDS             2311..4332
                     /locus_tag="VD737_03455"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXW09643.1"
                     /translation="MDRPAAHAAPRRQGAKRWELTLLVVITLLAALLMAWDWNWFKGP
                     IERRVATTTGREFRIEGLLDVDLGRNVIVRVEDMWLANAPWALTPEMARVDLLRVEVP
                     FWPLLRGERTLQRVDVVRPALLLERNRSGAANWQFREDERRASAKGWSFGELRVHDGR
                     LDVRDAPLGTDLRLTVDSSTPQPGDDSVRLLVRGSGRYRGHAFQLMGTADSPVELLER
                     ADAAYRIDFSARAGATRARVHGALRVPLDPKRFVIRSELRGNDLGDLYPLVGLAVPST
                     PPYRIEGLFERKGRVLTLRDLQGKIGDSDVAGVAGVDLSGDKPLIRADLRSTHLDLDD
                     LGGLIGLPPSIGEGESASPEQRAEAERRAAGPRLLPDKDYDLRKLAAANADFRLDAKH
                     VDAGKWPIDSLAMRLRLNDSILDLEPVELGFAGGRIAGDVQLDARQRTITAGASLGVH
                     ALDLERMWPNMQPPNVGRLNGRIDLQGEGNSLADMLATADGELRLGMGRGRFSNLLLE
                     LAGLDVAESLKFLLGKDKTVKLRCAYGDFTVEEGLVKTQAMVFDTSDTILFGEGTLDF
                     DGEKLALELRPEPKDFSPVSLRGPLQVGGTFKDPTFRPQAKSLLGRAAVAAALYAIAP
                     PAALLALIETGPGENIDCYTGKDKDDAGGDQRKRETTEARRKEGKKGSG"
BASE COUNT          686 a         1566 c         1572 g          636 t
ORIGIN      
        1 tccgtcgcac ggcctggtac tacaacgcct gggacagccg cgccgagggc atggccaccg
       61 cgatggagga aatcgtgctg cacgcggggt actacgacga caacccgcgg gcgcgcgaga
      121 tcgtctggat catgcttgcg cagcgggccg cgcgcggact cgcgtcgctg tacgcgcacg
      181 ccaacctgat ggacatccgg caggccaagg ccttccaggt ggagtggacc ccgcgcggct
      241 ggatgcgtcc cgacctggac ctgctgcact tcgaacagca gctgtacctg cgccagcccg
      301 gctacggcac cagctacgtg acgggcaagt acctgatcga cgagctggtc aaggatcgct
      361 cccaccagca gggcgccgcc ttcacgctcg gcggcttcat cgacgagttc aacgcggccg
      421 gcatgatgcc ggtgtcgctg atccgctggc agctgacggg cgacgcctcg atggtgccgg
      481 ggtgcgcggc cgccggggcc aggtgcgaac cggcttccgc gacgacccgg ccctgatcag
      541 gcctcgaggt ccacgaacgc gcgcagcgtg acgccgcggt cggcccacac gcgcgccgcg
      601 tcctcgacga tcgcgagcgc cgtgtcgagc gtttcggggg cgctgcggtg catgccgagc
      661 gcgcgtcgca tgacgagcac gtagcccggc gccggggcgt gccagccgag gtccgtcagg
      721 cagtcgaacc aggcgtccca gttgccgccg aaccagttcg gaaacgccat cgcgcggccg
      781 agccgctcca gcagcccggc cttgtcgtgg cagcccgcca ggtcggcgtc gaacagccgg
      841 taaccgagcg agtcggccag cgcgcccagc cccgcgacgc gctcgtcgtt gagcgtgacg
      901 cgttgcacgc tcaggcgctc gtcgtcgcgg cggggcgatt cgtcggtcgg catggtcacg
      961 gcggcgcagc ctcgatgcga cggaaactcc ggtagtgatc ctcggtgtac cagtacacct
     1021 cgggcggatc gccgcccgtg acgatccggc gcgcgccgcg gtcgcgcgag ccgggcgtct
     1081 cgacggtgta ctcacggtag tagccgcgtg gctgctgcgg caacagtcct tcgcgattct
     1141 ggaacacggt tccgtcacgg tcgtaccgga acgggccgcc gtccgcgatc gcctggagcg
     1201 tcgcgatcgc ctccggcggc aatccggtcg gctcggacgc gtcgggcggg gcctgcggtg
     1261 cttcgacgac cgcggacgga cgctcgtgcg caaaccagcc gttggccgcg acgagtccca
     1321 ccgccgcgag cagcacgaac agccacgggc gcgacacggc tcagtccggc aggaagccgt
     1381 ggctgccgag gaggctctgg ccgccgtcgc agacgacgac ggcgcccgtc acgtactgcg
     1441 cggcgtccga gcagaggaac gcggccaggt ccgcgatctc gcccttgcgc ccgtaacgcc
     1501 cgagcgggat cgagcgctcg agcttctcgc gcgcgcccgg cgccgcgccc agtcgccgca
     1561 tgccctcggt gtcctcgatc gggccgggca cgatcgagac gacgcgaatc ccgtcccggc
     1621 cccattcgac ggcgaggttg cgcgtgagca tgtccacccc ggcttttgcg gcgccgacgt
     1681 gtgactggcc gggcatcggc atgtaggcct gtggcgccga gatgttgacg atcgcggcgc
     1741 ccggcttgcg caggtgggcg tgcgccgcgc gcaggacgtt gaaggtgccg agcaggtcga
     1801 tgtcgacgac cgtgcgaaag cctttggccg acatgtcgag cgcgcgcgcg acgaagttgc
     1861 ccgcggcgcc ggagaccagc atgtcgatcg gcccgaagtg cgcggccgcc gcgctcagcg
     1921 ccgcttcgat agcgtcgtac tcgcgcacgt cgcaggcctg gccaaaggcg cgcgcgccgc
     1981 gcgcctgcag gtggcgcacg gccgtgtcga ccttctccgg cgaacggctc accaccgtca
     2041 cggcggcgcc caggtccgcg aaccgctcgg cgatgccgag attgatgccg ctcgtgccgc
     2101 ccgcgacgaa agcggtgcgt ccctcgaaca gcccgtccct gaacacgcca gccatgtgct
     2161 tttccttgcg cggaggatct cttcgcaaga ttaccctgcc tgtcgctccg accgcacccg
     2221 gttcgcttcg ccgcggtcag accccaccga cggccccggt ccgggctccg gggactgacg
     2281 ttgcgcgccc ggtggcgaac actgcggtgg atggatcgac cggccgctca tgcggcgccg
     2341 cgccggcaag gcgccaagcg ctgggaactc acgctgctcg tggtgatcac gctgctggcg
     2401 gccctgttga tggcctggga ctggaactgg ttcaagggac cgatcgagcg gcgcgtcgcg
     2461 accacgaccg gccgcgagtt ccgcatcgag ggcttgctcg acgtggacct cggccgcaac
     2521 gtgatcgtgc gcgtcgagga catgtggctc gcgaacgcgc cgtgggcgct cacgcccgag
     2581 atggcgcgcg tcgacctgct gcgcgtggag gtcccgttct ggccactgct gcggggcgag
     2641 cgcacgctgc aacgcgtgga cgtcgtgcgg ccggcgttgc tgctcgagcg caaccggagc
     2701 ggcgccgcca actggcagtt ccgcgaggac gagcggcggg cgtcggcgaa gggctggtcg
     2761 ttcggcgaac tccgtgtgca cgacgggcgg ctcgacgtcc gcgacgcccc gctcggcacc
     2821 gacctgcggt tgaccgtcga ttcgagcacg ccgcagcccg gcgacgacag cgtccggctg
     2881 ctggtgcgcg gctcgggtcg ataccgcggc cacgccttcc agctgatggg cacggcggac
     2941 tcgccggtcg agttgctcga gcgcgcggac gcagcctacc ggatcgactt ttcggcgcgc
     3001 gccggcgcga cgcgcgcgcg cgtgcacggc gcgctgcgcg taccgctcga tccgaaacga
     3061 ttcgtcatac gctccgaact gcgtggcaac gatctcggtg acctctaccc gctcgtgggc
     3121 ctcgccgtgc cctcgacccc gccgtaccgc atcgaaggcc tcttcgagcg caagggccgc
     3181 gtgctgacgc tgcgcgacct gcagggaaag atcggtgaca gcgacgtggc gggcgtggcc
     3241 ggcgtggacc tgagcggcga caagccgctg atccgcgccg acctgcggtc gacgcacctc
     3301 gacctcgacg acctcggtgg attgatcggc ctgccgccgt cgatcggcga aggcgaatcg
     3361 gcctcgccgg agcagcgtgc cgaggccgaa cgtcgcgccg ccggtccgcg tctgctgccc
     3421 gacaaggact acgacctgcg caagctggcc gcggcgaatg ccgacttccg cctcgacgcg
     3481 aagcacgtcg acgccggcaa gtggccgatc gactcgctcg cgatgcgcct gcgcctgaac
     3541 gattcgatcc tcgatctcga gcccgtcgaa ctcggcttcg ccggcggtcg gatagcgggc
     3601 gacgtacagc tcgacgcgcg gcagcgcacg atcaccgcag gcgccagcct gggcgtccac
     3661 gcgctggacc tcgagcgcat gtggccgaac atgcagccac cgaacgtcgg tcggctgaac
     3721 ggtcgcatcg acctgcaggg tgaaggcaat tcgctggccg acatgctcgc caccgccgac
     3781 ggcgagctgc ggctcggcat ggggcgcggg cggttcagca acctgctgct cgagctggct
     3841 gggctcgacg tcgccgaatc gctgaagttc ctgctcggca aggacaagac cgtgaagctg
     3901 cgctgcgcct acggcgactt cacggtcgag gaaggcctcg tgaagacgca ggcgatggtc
     3961 ttcgacacga gcgacacgat cctgttcggt gaagggacgc tggacttcga cggcgagaag
     4021 ctcgcgctgg agctgcggcc cgagcccaag gacttcagcc cggtgtcgct gcgcgggccg
     4081 ctgcaggtcg gcggcacgtt caaggacccc acgttccggc cacaggccaa gtcgctgctc
     4141 ggccgtgccg cagtcgcggc ggccctctat gcgatcgcac cacctgcagc gctgctcgcg
     4201 ctgatcgaga ccgggcccgg cgaaaacatc gactgctaca ccggcaagga caaggacgac
     4261 gccggcggag accagcgcaa gcgcgagacg acggaagcgc ggaggaagga ggggaagaaa
     4321 ggaagcggtt agtgtccagt gtctagtgtc tagtaagaat aagaagcgga tccgcaacgc
     4381 cgacgagtcg gtctcctact aaccgctaga cactaaacac tagacacttc tttccgcatc
     4441 agcgtgctct tcccgaacag
//
LOCUS       DASPFR010000137         3385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_527016, whole genome shotgun sequence.
ACCESSION   DASPFR010000137 DASPFR010000000
VERSION     DASPFR010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527016"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="VD737_03460"
     CDS             complement(<1..1339)
                     /locus_tag="VD737_03460"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXW09644.1"
                     /translation="MIARTLAFVTLLGATSVCVAQAPAPTPAGVYTEAQAARGKQTYD
                     RYCAQCHHLTLRGSGHGTALEGRPFLQRWGTRTLGELSTYIATQMASNLPRGTPPATF
                     DDLAAHILRVNGAAYGDQELVASNAVAVGAAIMGAAWDPVALHGPEGSDGGKRESWSG
                     AATIAEAAQKAGTWTNRETPALAPVTDAMLRDPPPGDWLSFRRTLDGWGYSPLAQINR
                     DTVGRLRLAWAITMREGSNQPTPLVHDGIMFLTHPGNVIQALDAANGDLIWEYQYEYP
                     PEAQTLGGPTRNIAIYQDKLFLATYDAALVALDARTGRQLWRTVKADYRKGFTHTAGP
                     IIADGVVVSGINGCERFKPEGCFITGHDPDTGRELWRTSTIALPGDPNDQTWGGVPVS
                     RRGGGDNWFAGSYDPELELFFLGTSQPKPWVAASRGMSPRDAALYTNSTLALEP"
     gene            1439..2272
                     /locus_tag="VD737_03465"
     CDS             1439..2272
                     /locus_tag="VD737_03465"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXW09645.1"
                     /translation="MARTIVDAWAQHPTPRHLDHPMFESLRRWTRGLGGPALTAGEQW
                     PIETTLAAMDAGGISLSLLSAWVGPRGDLISNDEVASFVRQAPQRLAGVGSLDVSRPR
                     EAVRELRRCVRDLGFKAVRVLPWLWEMPPTHARLYPIYAECCELGVPFCTQVGHTGPM
                     MPSEYGRPIPYIDQVAIDFPELVIVGGHIGYPWTDEMIAVATKHANVYIDTSAYTVAR
                     YPGALVDYLRGHGRDKVLFGTNWPMVPPVKALEALPTLALDEAVEAKFLAGNAQRVFR
                     L"
     gene            complement(2358..2870)
                     /locus_tag="VD737_03470"
     CDS             complement(2358..2870)
                     /locus_tag="VD737_03470"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HXW09646.1"
                     /translation="MVSTRRVLVPFAAALFAIAGAVHANPVYKASMDGKQMLPEPIQT
                     VATGAVEFVVSADGKSIAYKVSVDRLTNASAADVHLGPPSQNGPVIARLYPNGKGGKK
                     GEYSGVLTEGRLTGADLVGPLTGAGIGDLLAELAAGNVYVNVHTNDGVEPANSGPGDF
                     RLGEIRGQLK"
     gene            complement(3014..>3385)
                     /locus_tag="VD737_03475"
     CDS             complement(3014..>3385)
                     /locus_tag="VD737_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HXW09647.1"
                     /translation="GHAQQVPLRNPFSLTSAIKFGALFAVVLLVVKIVQAQAPSAGMY
                     VVAAIAGTTDVDAITLSMAQYARTSSPQVAAHAITIALLSNTVVKAGMVLALGHPSIR
                     KWTILATTAIVGAGVAAIVLT"
BASE COUNT          466 a         1151 c         1228 g          540 t
ORIGIN      
        1 ggggctcgag cgcgagcgtc gagttcgtgt acagcgcggc gtcgcgcggc gacatgccgc
       61 ggctcgccgc cacccacggc ttcggttgcg acgtgcccag gaagaacagc tcgagctcgg
      121 ggtcgtagct gcctgcaaac cagttgtcac cgccgccacg ccggctgacg ggcacgccgc
      181 cccaggtctg gtcgttggga tcgccgggaa gcgcgatcgt ggacgtgcgc cagagctccc
      241 gtccggtgtc tgggtcgtga ccggtgatga agcagccctc gggcttgaag cgctcgcagc
      301 cgttgatccc gctcacgacg acgccgtccg cgatgatcgg gccggccgtg tgcgtgaatc
      361 ctttcctgta gtcggccttg accgtgcgcc acagttgccg gccggtgcgc gcgtcgagcg
      421 ccaccagggc cgcgtcgtac gtggccagga acagcttgtc ctggtagatc gcgatgttgc
      481 gcgtcgggcc gccgagcgtc tgcgcctcgg gcggatactc gtactggtac tcccagatca
      541 ggtcgccgtt cgccgcatcg agcgcctgga tcacgttgcc cgggtgtgtc aggaacatga
      601 tgccgtcgtg cacgagcggg gtgggctggt tgctgccctc gcgcatcgtg atcgcccacg
      661 cgaggcgcag gcgcccgacg gtgtcgcggt tgatctgcgc gagcgggctg tagccccagc
      721 cgtcgagcgt gcggcggaag ctcagccagt cgccgggcgg cgggtcgcgc agcatcgcgt
      781 ccgtgacggg cgcgagagcc ggcgtctcgc gattggtcca cgtgccagcc ttctgtgccg
      841 cctcggcgat cgtcgcggca ccgctccagc tctcgcgctt gccgccgtcc gagccttcgg
      901 gcccgtgcag cgccaccgga tcccaggcgg cgcccatgat cgccgcgccg accgcgacgg
      961 cgttcgaggc gacgagttcc tggtcgccgt aggccgcgcc gttgactcgc aggatgtgcg
     1021 cggccaggtc gtcgaaggtg gcggggggcg tgccgcgcgg caggttggaa gccatttgcg
     1081 tggcgatgta cgtgctcagc tcaccgagcg tgcgcgtgcc ccagcgctgc aggaacggcc
     1141 tgccctcgag cgccgtcccg tggccgctgc cgcgcagcgt caggtgatgg cactgcgcgc
     1201 agtaacggtc gtaggtctgc ttgccgcgtg cggcctgggc ttcggtgtag acgccggccg
     1261 gggtcggggc cggggcctgt gcgacgcaca cggaggtggc gcccagcagc gtgacgaagg
     1321 caagcgtgcg cgcgatcatg gtgcggtttc cttccgggtc ggcggcctgc atcggccagg
     1381 cccggcgaat atagcgacgt tgcttgttgg ccgacaacaa atgatcgagg actcaccagt
     1441 ggcacgcacg atcgtcgacg cctgggcaca gcacccgacc ccgcgccacc tggatcaccc
     1501 catgttcgag tccctgcgcc gctggacgcg cgggctcggc ggccccgcgc tcacggccgg
     1561 cgagcaatgg ccgatcgaga ccaccctcgc ggcgatggat gcgggcggca tctcgctcag
     1621 cctgctctcg gcctgggtcg ggccccgcgg cgacctgatc tcgaacgacg aggtcgcctc
     1681 gttcgtacgc caggcaccgc agcgcctggc gggcgtcggc tcgctcgacg tcagccgtcc
     1741 gcgcgaggcg gtgcgcgaac tgcgccgctg cgtgcgcgat ctcggcttca aggccgtccg
     1801 cgtgctgccc tggctctggg agatgccgcc gacgcacgcg cgcctgtatc cgatctatgc
     1861 cgagtgctgc gagctcggcg tgccgttctg cacgcaggtg ggccacacgg ggccgatgat
     1921 gccgtcggag tacgggcgac cgatcccgta catcgaccag gtcgcgatcg acttcccgga
     1981 actcgtgatc gtcggaggcc acatcggcta cccgtggacc gacgagatga tcgcggtggc
     2041 caccaagcac gcgaacgtct acatcgacac ttcggcgtac accgtcgcgc gctatccggg
     2101 tgcgctcgtc gattacctgc gcggacacgg gcgcgacaag gtgctgttcg gcacgaactg
     2161 gccgatggtg ccgccggtca aggcgctcga ggcactgccg acactcgcgc tcgacgaggc
     2221 cgtggaggcg aagttcctgg ccggcaacgc acagagggtg tttcgcctct gaccacgccc
     2281 cctcccgcgc gtgcgggaga ggggtggggg ccctggctgc agggccgctg gcaggccggc
     2341 acgagccggc agttcgtcta cttcaactgg ccgcggatct cgccgagccg gaaatcgccg
     2401 ggcccggagt tcgccggctc gacgccatcg ttggtgtgca cgttgacgta cacgttgccc
     2461 gccgcgagct ccgcgagcag gtctccgatg cccgcgcccg tcagcgggcc gaccaggtcg
     2521 gcacccgtca ggcggccttc ggtgagcaca ccgctgtact cgcccttctt cccgcccttg
     2581 ccgttcggat agagcctggc gatcacgggc ccgttctgcg acgggggacc gaggtgcacg
     2641 tcggccgcgg acgcgttggt gaggcgatcg accgagactt tgtaggcgat gctcttgccg
     2701 tcggcgctca cgacgaactc gaccgcgccg gtcgcgacgg tctggatcgg ctccggcagc
     2761 atctgcttgc cgtccatcga tgccttgtag accgggttgg cgtggacggc gccggcgatt
     2821 gcgaaaagcg cggccgcgaa gggaaccagg acgcgacggg tgctgaccat gtgacctcca
     2881 gctctccgag tcacggcgca atgaaatgcc tgcgggcgcg cgccgggaat acgtagttgc
     2941 tgagaggtcc tttcgcaccg cgacggggcc ggcgcacggt ccggccggtg ccgggcgcac
     3001 cggtgcgccg cgatcaggtg aggacgatgg ccgcgacgcc cgccccgacg atcgccgtgg
     3061 tggcgaggat cgtccacttg cggatcgacg ggtggcccag agcgagcacc atgccggcct
     3121 tcaccacggt gttgctcagc aatgcgatcg tgatcgcgtg cgcggcgacc tgtggactgc
     3181 tggtgcgtgc gtactgggcc atggacagcg tgatcgcgtc gacgtcggtg gtgccggcga
     3241 tggcggcgac cacgtacatg ccggccgaag gtgcctgggc ctgcacgatc ttcacgacca
     3301 gcagcacgac cgcgaagagc gcgccgaact tgatggcgga ggtcaggctg aacgggttgc
     3361 gcagcggcac ctgctgcgcg tggcc
//
LOCUS       DASPFR010000138         6324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1015620, whole genome shotgun sequence.
ACCESSION   DASPFR010000138 DASPFR010000000
VERSION     DASPFR010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015620"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="VD737_03480"
     CDS             <1..1145
                     /locus_tag="VD737_03480"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09648.1"
                     /translation="QQGLARREQRSPVRWRRAAGRIVQLERMLVQDGIAIEKVHLHVS
                     ESTQRKRLDVLAGAKATRWRVTGEDRWLSRHYAEVERALERGLDATEHAAAPWHLVDG
                     TDEQRRVEDVAGRLLQALSNGPADVAARTDRAHVAPRRPRKSGFPSAGAGATGRGDDH
                     YDRELERLQGRLARLTRRKRFGDHAVVAVFEGMDAAGKGGAIRRVVAALDARQYRVVP
                     ISAPSPEERARPYLWRFWRRLPPRGNFTIFDRSWYGRVLVERVRNFAAARDWSRAYDE
                     IVEFERQLDEHGVIVAKFWLAVSRDEQLARFEERDRNPLKRHKVDPEDWVNREHWSQY
                     QRAAEDMIARTDTPHGPWTVIAADDKREARLQVLSTLVDRIDAALD"
     gene            1172..2125
                     /locus_tag="VD737_03485"
     CDS             1172..2125
                     /locus_tag="VD737_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09649.1"
                     /translation="MTHVALVSADAALTLDEDMPPLVEAFRRLGARVATPSWDAPDVD
                     WSRFDLVLLRSTWDYVERLDEFLAWADACGRCTRLFNPAAVVAWNTDKHYLAELHAAG
                     VAVVPTRFAEPGTRAAASLQAFLAGGAGSLTAGAPCAFDQYVVKPTVGAGSRDAARYR
                     RGDDDARALAHLSRLVEVERRSAMLQPYLASVDTEGETALMYLGGSPSHAIRKGPLLR
                     LDDGLVAGLFAPEDIRPRVPSHDEQVLAEATLRSLPFESPLYARVDMVRDAWGNPVLL
                     ELELTEPSLFLAHAPGSADRLARAALSRAAIAAEEVTDRST"
     regulatory      2309..2395
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2466..3110
                     /locus_tag="VD737_03490"
     CDS             2466..3110
                     /locus_tag="VD737_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09650.1"
                     /translation="MAGLERISCGPLVETAALDPARRAARLHRQRRRHSSVRAVVLGV
                     TLLLALLFLLGATPSRAEGTERQQMKGLDEQVQEIKSDVLEIAAELGRLEEKLLYPSN
                     TQVAVFVAVAPQDASRFDAVRIQIDGQAVAHYIYSFKELDALRRGGVQRLYVGNLPTG
                     DHRIDVQVDGKAQGGADFSHTEQFTFSKGVEPKLVGLTLAGPDSGRAPIEIGNW"
     gene            3117..5003
                     /locus_tag="VD737_03495"
     CDS             3117..5003
                     /locus_tag="VD737_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXW09651.1"
                     /translation="MMRAPHRLLGTVVTLALATPLAVAEDRLQDLHFGEALYHAYQGE
                     FFDALERLDTELGQHYGLDEPALDTLYPHIRTAEFDVGDFELNYRMHHRAGRAIKAVL
                     EADVDDAIRNEAAYRLARIHFQKDQLDEALHALGRIRGEVPGSIRDDIEFLRANILLA
                     TGRPREAAEVLQPLQGSASLAGFSAYNLGIALLQQGDQAGAVAQLDRAGQVRATTPST
                     QAIRDKSNFVLGTLLFESGDFGRAGQSLDRVRLEGPFSNQALLRTGWAERSAEHYDRA
                     LVPWQLLAEREPTDAAVQEALLAVPHAYAALEVHGRAAVLYGRALETYGQQIERVDAS
                     IDSIRDGRFLDALVREEINQDQTWVVRLRSLPEAPETYYLTELMASHDFQTSLQNYLD
                     LEDLRARLKAWQSSFDAFDDMTALRARYYEPLLPEVDARFRDVDAQMKLRLEQRAHVA
                     DRLRDMLTAPRPEYLATTEERVDLEHLAALRRQLEGDAGEPAEALRARIARVEGRLTW
                     RLRTEYHERLTQAHVHLVELDADVATMNARYQAYVRSRQAATHSYVGYDVQVARLRER
                     VKGALQRVDLAMARQGHLIETAAIDQLKVRRERLVDLQTQARYAVADSYDRASRLQTA
                     GVQP"
     gene            5000..>6324
                     /locus_tag="VD737_03500"
     CDS             5000..>6324
                     /locus_tag="VD737_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXW09652.1"
                     /translation="MKFRLTVPVVLVPLLMAGCAETPERDTLADLRKAKPDLQDVKVE
                     QGLDLAMAGYRRYLEETPQSVLTPEAMRRLADLQIEKQFGLRAGDGKPREMAAPERVA
                     VAAAGEPRIVATAAGKPVAAESDGDFERRTTAQASVIPAVDVERSAATVAGVEEPSGP
                     LEAIALYDKLLAEYPNYEHADKVLYQKARAYDELGRTEDAMQTMEQVMARYPHSAHYD
                     EVQFRRAEFFFTRRQFRDAETAYASIITLGPASSYYELALYKLGWTLYKQEFYEEALH
                     RYMALLDHKVSIGYDFDQQHEVDDERRVTDTFRVISLSFSNLGGPDVVREYFSTYGQR
                     DYEDRVYGNLGEYYLDKLRYDDAARAYRSFVELYPYHRVSPRFSMRVIDIFTKGGFPK
                     LVLESKKDFATRYGLASEYWRHFAPAENPEVLAFLKSNLKDLANHYHAQ"
BASE COUNT         1038 a         2211 c         2185 g          890 t
ORIGIN      
        1 tgcagcaggg gctcgcacgg agggagcagc gatcgccggt gcggtggcgg cgggccgccg
       61 ggcgcatcgt ccagctcgaa cggatgctgg tgcaggacgg catcgcgatc gagaaggtgc
      121 acctgcacgt ctcggagtcc acacagcgca agcggctcga cgtgctcgcc ggagccaagg
      181 ccacgcgctg gcgcgtgacg ggtgaggacc gctggctgtc gcgccattac gcggaggtcg
      241 agcgtgcgct cgaacgcggc ctggacgcca ccgagcatgc ggcggcgccc tggcacctgg
      301 tcgacggtac cgacgagcag cgccgcgtcg aggacgtggc cgggcgcctg ctgcaggcgc
      361 tgtccaatgg gcccgccgac gtggctgcgc gcaccgaccg ggcgcacgtt gcgcccagga
      421 gaccacgcaa gtccggcttc ccgtcggcgg gtgcaggggc cacgggacga ggcgacgacc
      481 actacgaccg cgagctcgag cgcctgcagg gccggctcgc gcgactgacg cgacgcaagc
      541 gcttcggcga ccacgcggtc gtcgcggtgt tcgaagggat ggacgccgcg ggcaagggcg
      601 gcgcgatccg gcgcgtggtc gccgcactcg acgctcgcca gtaccgcgtg gtgccgatct
      661 cggcgccgtc gccggaggaa cgcgcgcgac cctacctctg gcgcttctgg cgccgactgc
      721 cgccgcgcgg caacttcacg atcttcgacc gcagctggta cggccgcgtg ctggtcgagc
      781 gcgtgcgaaa cttcgcggcg gcgcgggact ggagccgggc gtacgacgag atcgtcgagt
      841 tcgagcgcca gctcgacgag cacggcgtga tcgtcgcgaa gttctggctg gccgtgagtc
      901 gcgacgagca gctcgcgagg ttcgaggaac gcgaccgcaa cccgctcaag cgccacaagg
      961 tcgacccgga ggactgggtc aaccgcgaac actggtcgca gtaccagcgc gcggccgagg
     1021 acatgatcgc gcgtaccgac acgccgcatg gcccgtggac cgtgatcgcg gcggacgaca
     1081 agcgcgaggc gcgcctgcag gtactgagca cgctggtcga ccgcatcgac gcagcgctcg
     1141 actgatcacg gcaaccagag gaacgtctgc cttgacccac gttgccctcg tgagcgctga
     1201 tgccgcgctc accctcgacg aagacatgcc gccgctcgtc gaggccttcc ggcggctcgg
     1261 cgcacgggtc gcgacgccca gctgggacgc gccggacgtc gactggtcgc gcttcgacct
     1321 ggtgctgctg cgatcgacct gggactacgt ggagcgcctc gacgagtttc tcgcgtgggc
     1381 ggacgcgtgc ggccggtgca cgcggctgtt caatccggcc gccgtcgtgg cctggaacac
     1441 cgacaagcac tacctggccg agttgcacgc cgccggcgtg gccgtggttc cgacgcggtt
     1501 cgccgagccc gggacccggg cggcggcatc gctgcaggca ttcctggcgg gcggggccgg
     1561 gtcgctgacc gccggcgcgc cgtgcgcctt cgaccagtac gtggtcaagc cgacggtggg
     1621 tgccgggtcg cgggacgcgg cgcgttaccg ccgcggcgac gacgacgctc gcgcgctcgc
     1681 gcacctgagc cgactcgtgg aggtcgagcg gcgcagcgcc atgctgcagc cctacctggc
     1741 cagcgtggac accgaagggg agaccgcgct gatgtacctg ggcggatcgc cgagccatgc
     1801 catccgcaag ggcccgctac tgcggctcga tgacgggctc gtcgccggac tgttcgcgcc
     1861 ggaagacatc cgcccgcgcg tgccgagcca cgacgagcag gtcctcgcgg aggcgacgct
     1921 ccggtccctg ccgttcgagt cgcccctata tgcacgcgtg gacatggtgc gggatgcctg
     1981 gggcaatccg gtgctgctgg aactggaact caccgaaccg tcgctgttcc ttgcgcacgc
     2041 gccgggcagc gccgaccgcc tcgctcgggc cgccctgtcg cgcgcggcca tcgcagccga
     2101 agaggtgacg gatcgctcga cgtgaccgac cgcgcgcgcg gtgccacgtg cgcaaggtcg
     2161 actgacagaa gtgtgacagg cttcggcatc tgtgacacgg cacgtccgga agtgacgctc
     2221 cgatgtgaca tagcgtgtca aacacgcgca ggaatgggat tcacgtcatc ccatcatctg
     2281 taaaacattc cgatagttcc gcccgcgatt cagtcaaggg caaagccatc gaaaggtggc
     2341 gacgcaaagt ctccggtcta aagggctgcg gccccacgac agcgggaccg ccgaatcgga
     2401 tccggcagga tcctgtcacg gcatgccatg cccgctcgat cgcagggagc gacacgaggt
     2461 gacacatggc cggacttgag cggatttcct gcggtccact cgtcgagacc gccgcgctcg
     2521 accccgcgcg gcgcgccgcg cgcctccacc gccagcgacg tcggcactcg tccgtgcggg
     2581 ccgtcgtgct cggcgtgacc ctgctgctgg cgctgctgtt cctgctgggc gcgaccccga
     2641 gccgtgccga gggcaccgag cgccagcaga tgaaggggct cgacgaacag gtccaggaga
     2701 tcaagtccga cgtgctcgag atcgcggccg agctgggtcg cctcgaagag aagctgctgt
     2761 atccctccaa cacgcaggtc gcggttttcg tcgcggtcgc gccacaggat gcttcgcgct
     2821 tcgacgccgt gcgcatccag atcgacggcc aggcggtcgc gcactacatc tacagcttca
     2881 aggagctcga cgcgctgcgt cgcggcggcg tgcagcgcct gtacgtcggc aacctgccga
     2941 ccggcgatca caggatcgac gtgcaggtcg acggcaaggc gcagggcggt gcggacttca
     3001 gccacacgga acagttcacg ttcagcaagg gcgtcgagcc caagctggtc ggactgacgc
     3061 tggcaggtcc cgactcgggc cgggcgccga tcgagatcgg gaactggtga cctgaaatga
     3121 tgcgagcgcc gcaccggctg ctcggcaccg tcgtcacgct ggcgctggcg acgccgctcg
     3181 ccgtggccga ggaccgcctg caggacctgc atttcggcga ggcgctctat cacgcctacc
     3241 agggcgagtt cttcgacgcg ctcgagcggc tcgacacgga gctcggccag cattacgggc
     3301 tcgacgagcc agccctggac acgctctacc cacatatccg gaccgccgaa ttcgacgtcg
     3361 gcgatttcga gctcaactac agaatgcacc atcgcgccgg ccgcgcgatc aaggccgtgc
     3421 tcgaggccga cgtcgacgac gccatccgta acgaggcggc ataccggctc gcgcgcatcc
     3481 acttccagaa ggaccagctc gacgaggcgc tgcacgcgct cggccgcatc cgtggcgagg
     3541 tgcccgggtc gatccgggac gacatcgagt tcctgcgcgc gaacatcctg ctcgcgaccg
     3601 gtcgtccgcg cgaggcggcc gaggtcctcc agcccctgca gggctccgcc tcgctggccg
     3661 gcttcagcgc ctacaacctg ggcatcgccc tgctgcagca gggcgaccag gcgggcgcag
     3721 tggcgcagct cgaccgtgcc gggcaggtca gggcgaccac cccgtccacg caagcgatcc
     3781 gcgacaagtc caacttcgtc cttggcacgc tgctgttcga gtccggcgac ttcggccgcg
     3841 ccggacagtc cctcgatcgc gtgcggctcg aaggcccgtt ctcgaaccag gcgttgttgc
     3901 ggaccgggtg ggccgaacga tcggccgagc actatgaccg tgcgctcgta ccgtggcagc
     3961 tcctcgccga gcgcgaaccg acggacgccg cggtccagga ggcgctgctc gccgtgcctc
     4021 acgcctacgc cgcgctcgag gtgcacggcc gggcggccgt gctctacggg cgcgccctcg
     4081 agacgtacgg gcagcagatc gaacgcgtcg acgcgtcgat cgacagcatc cgcgacggtc
     4141 gcttcctcga cgcgctcgtc cgcgaggaga tcaaccagga ccagacctgg gtcgtgcgcc
     4201 tgcgctcgct gccggaggcg cccgagacct attacctcac ggagttgatg gcgtcgcacg
     4261 acttccagac ctcgctgcag aactacctcg acctcgagga cctgcgcgcg cggctgaagg
     4321 cctggcaatc gagcttcgat gcgttcgacg acatgacggc gctgcgcgcg cgctactacg
     4381 agccgctgct ccccgaggtc gacgcacggt tccgtgacgt cgacgcccag atgaagctgc
     4441 ggctcgagca gcgcgcgcac gtcgccgacc gcctgcgtga catgctcacc gcgccgcgac
     4501 cggaatacct cgcgacgacc gaggaacgcg tcgacctgga acacctggcc gcgctgcgac
     4561 ggcagctcga aggcgatgcg ggcgagcctg ccgaagccct gcgcgcgagg atcgcgcgag
     4621 tcgagggccg gctgacctgg cgcctgcgca cggaatacca cgagcggctg acgcaggcgc
     4681 acgtgcacct cgtcgaactc gatgccgacg tcgccacgat gaacgcgcgc taccaggcgt
     4741 acgtacggtc gcgccaggcc gcgacgcaca gctacgtcgg ctacgacgtg caggttgcgc
     4801 gcctgcgcga gcgcgtgaag ggcgccttgc agcgcgtgga cctcgcgatg gcgcgccagg
     4861 gtcacctgat cgagaccgcc gcgatcgacc agctcaaggt gcgtcgcgag cgactcgtcg
     4921 acctgcagac gcaggcccgc tacgcggttg ccgacagcta cgaccgcgcg tcgcgcctgc
     4981 agaccgccgg ggtgcagcca tgaagttccg gcttacggtg cccgtggtgc tggtcccgct
     5041 gttgatggcc gggtgcgccg agacgccgga gcgcgacacg cttgcggacc tgcgcaaggc
     5101 gaagcccgac ctgcaggacg tcaaggtcga gcagggcctc gacctggcaa tggctggcta
     5161 ccgacggtac ctcgaggaaa cgccacagag cgtgctgacg cccgaggcga tgcgccggct
     5221 tgcggacctg cagatcgaga agcagttcgg cctgcgcgcg ggcgacggca agccgcgcga
     5281 aatggccgcc ccggagcgcg tcgccgtcgc ggccgcaggc gagcctcgca tcgtggcaac
     5341 ggccgcgggc aaaccggtcg ccgcggaatc ggacggcgat ttcgagcgtc gcacgacggc
     5401 gcaggcttcg gtgatccccg cagtcgacgt ggagcggtcc gcagcgacgg tggccggcgt
     5461 cgaggagcct tccgggccgc tcgaggcgat cgcgctctac gacaagctgc tcgccgagta
     5521 cccgaactac gaacacgcgg acaaggtgct ctaccagaag gcgcgcgcct acgacgaact
     5581 cggccgcacc gaagacgcga tgcagacgat ggagcaggtg atggcgcgct acccgcactc
     5641 ggcgcactac gacgaggtgc agttccgccg cgccgagttc ttcttcacgc gtcgccagtt
     5701 ccgggatgcg gagacggcct acgcgtcgat catcacgctc ggccccgcgt cctcgtacta
     5761 cgagctcgcg ctctacaagc tcggctggac cctctacaag caggagttct acgaggaagc
     5821 gctgcaccgc tacatggccc tgctcgacca caaggtgtcg atcggctacg acttcgacca
     5881 gcagcacgag gtggacgacg agcggcgcgt caccgacacc ttccgcgtca tcagcttgag
     5941 cttcagcaac ctcggtggcc ccgacgtcgt ccgcgagtac ttctcgacct acgggcagcg
     6001 cgactacgag gaccgcgtct acggcaacct cggcgagtac tacctcgaca agctgcgcta
     6061 cgacgatgcg gccagggcgt accgctcgtt cgtcgagctg tatccctacc accgcgtgtc
     6121 gccgcgcttc agcatgcgcg tgatcgacat cttcacgaag ggcggcttcc cgaagctggt
     6181 gctcgagtcg aagaaggact tcgcgacgcg ctacggcctg gcctccgagt actggcgcca
     6241 cttcgcgccc gccgagaacc cggaggtgct ggcgttcctg aagtcgaact tgaaggacct
     6301 cgcgaatcac taccacgccc agta
//
LOCUS       DASPFR010000139         2849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_284618, whole genome shotgun sequence.
ACCESSION   DASPFR010000139 DASPFR010000000
VERSION     DASPFR010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284618"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VD737_03505"
     CDS             <1..354
                     /locus_tag="VD737_03505"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HXW09653.1"
                     /translation="LPSWVIVSCYVAIAFGTMFGGWRIVKTMGQKITKLRPVGGFAAE
                     TGGAITLFLASSLGIPVSTTHTITGAIIGVGSAHRTRAVRWGIAGNIVFAWIVTMPAA
                     ALMAALAYYLGRLLI"
     gene            398..2437
                     /locus_tag="VD737_03510"
     CDS             398..2437
                     /locus_tag="VD737_03510"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005171820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HXW09654.1"
                     /translation="MPYPHLLAPLDLGFVRLRNRVLMGSMHTGLEDHARHFPRLEAYF
                     AERARGQVGLIVTGGFAPNVEGWAAPFSGRLTTAAGAARHRPITRAVHAEGGHIALQI
                     LHTGRYGYHPFAVSASRSKSPISPFTPRALGARGIERQIRAFVRCAVLAREAGYDGVE
                     VMGSEGYFINQFLVEHVNRRDDDWGGTYQNRMRLPVEIMERTREAVGRDFIIIYRLSM
                     LDLVPDGSDWSEVVQLARAIERAGATIINTGIGWHEARIPTIATSVPRAAFAWVTRKL
                     KGEVGIPLVATNRINTPAVAEQLLADGSADMVSMARPLLADPEFVAKAAAGRADEINT
                     CIACNQACLDHVFRRQVASCLVNPRAGRETELRYEPAAVRKRVAVVGAGPAGLACATV
                     LADRGHDVDLYEAGDDVGGQFNLAMRVPGKEEFRETLRYFRRRLEVTGVRLHLRVRAS
                     PESLLARDYDEIVLATGVTARDPAIAGTAEQRAAGRVLDYADALLARRPIGPRVAVVG
                     AGGIGFDVAEYLVTGAHSPSLDLALWQREWGVTDPALRRGGLAAPEVEPPARKVYLLQ
                     RKATPHGKGLGKTTGWIHRASLKMKRVEMVGDVRYERVDADGLHVSFGEARERPTVFA
                     VDTVVLCAGQESVRDLEAPLRAAGRRVHLVGGAEVAAELDAQRAIDQASRLAARL"
     gene            complement(2451..>2849)
                     /locus_tag="VD737_03515"
     CDS             complement(2451..>2849)
                     /locus_tag="VD737_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HXW09655.1"
                     /translation="QILWVSSEQLARAAAVTAVFLAVWFRFRASLGTVGFYVAFSIMV
                     TTSVQLVGVYLVFTTLIVPALAVYRHARGRQLWLGYALAFAAYVVGLGVSLVSDLPSS
                     PVIVWAMALMGIGLHLTARAGNGAALREAA"
BASE COUNT          416 a          998 c         1029 g          406 t
ORIGIN      
        1 ctgcccagct gggtcatcgt cagctgctac gtcgcgatcg cgttcggcac catgttcggc
       61 ggctggcgca tcgtgaagac catgggccag aagatcacca agctgcgtcc ggtcggtggc
      121 ttcgcggccg agaccggagg cgcgatcacg ctgttcctcg cgtcgagcct ggggattccg
      181 gtgtcgacga cgcacacgat caccggcgcg atcatcggtg tcggatcggc gcaccggacg
      241 cgcgcggtac gctggggcat cgcaggcaac atcgtcttcg cctggatcgt cacgatgccg
      301 gccgcggcgc tgatggccgc gctggcctat tacctcggac gcctgctgat ctgacggtcc
      361 cctgccactt ccagaagaaa cccgcgaggg ttcgtcgatg ccgtatccgc acctgctcgc
      421 cccgctcgac ctcgggttcg tccggctgcg caaccgcgtg ctcatggggt cgatgcacac
      481 tggcctcgaa gaccacgcca ggcacttccc gcggctggag gcctatttcg cggagcgcgc
      541 gcgcggccag gtcggcctga tcgtcactgg cgggttcgcg ccgaacgtcg aaggctgggc
      601 cgcgccgttc tcgggacggc tcacgacggc ggcgggcgcc gcgaggcacc ggccgatcac
      661 ccgggccgtg catgccgaag gcgggcacat tgcgctgcag atcctgcaca ccggtcgcta
      721 cggctatcac ccgttcgcgg tctcggcgtc gcgatcgaag tcaccgattt cgccgttcac
      781 gccgcgcgcg ctgggcgcac gcggcatcga gcgccagatc cgcgcgttcg tgcgctgcgc
      841 cgtgctcgct cgtgaggccg gctacgacgg cgtcgaggtc atgggctcgg aggggtactt
      901 catcaaccag ttcctggtcg agcacgtgaa ccggcgtgac gacgactggg gcggtacgta
      961 ccagaaccgc atgcggctgc cggtcgagat catggagcgg acgcgcgagg cggtcgggcg
     1021 cgacttcatc atcatctacc ggctgtccat gctcgacctc gtgccggacg gcagcgactg
     1081 gagcgaggtc gtgcagctcg cgcgggcgat cgagcgcgcc ggcgcgacca tcatcaacac
     1141 cggcatcggc tggcacgagg cgcgcatccc gacgatcgcg acgtcggtgc cgcgcgcggc
     1201 gtttgcgtgg gtcacgcgca agctcaaggg cgaggtcggc atcccgctcg tcgccaccaa
     1261 ccgcatcaac acgcccgcgg tggccgagca gctgctcgcc gacggctcgg ccgacatggt
     1321 ctcgatggcg cgaccgctgc ttgccgaccc cgagttcgtc gccaaggcgg cggccgggcg
     1381 cgccgacgag atcaacacct gcattgcgtg caaccaggcg tgcctcgacc acgtgttccg
     1441 gcggcaggtc gcgtcgtgcc tggtcaatcc ccgcgccggt cgcgagaccg agctgcgcta
     1501 cgaaccggcg gccgtgcgca agcgggtcgc cgtggtcggc gccggcccgg cgggactggc
     1561 gtgcgcgacc gtgctcgccg accgtggtca cgacgtcgac ctctacgagg caggcgacga
     1621 cgtcggcggg cagttcaacc tcgcgatgcg cgtgcccggc aaggaggagt tccgcgagac
     1681 gctgcggtac ttccgccgtc ggctcgaggt gaccggcgtg cggctgcacc tgcgggtgcg
     1741 cgcgtcgccc gagtcgctgc tcgctcgaga ctacgacgag atcgtgctgg ccaccggcgt
     1801 gacggcgcgc gatccggcga tcgcaggcac ggccgagcag cgcgcggcgg gtcgcgtgct
     1861 cgactacgcc gacgccctgc tcgcgcgtcg cccgatcgga ccgcgcgtgg ccgtggtcgg
     1921 tgcgggcggc atcggcttcg acgtcgcgga atacctcgtg accggtgcgc attccccgtc
     1981 gctcgacctt gcgctgtggc agcgcgaatg gggcgtgacc gatcccgcgc tgcgtcgcgg
     2041 cggcctcgcg gcgcccgagg tcgagccgcc ggcacgcaag gtctacctgc tgcagcgcaa
     2101 ggccacgccg cacggcaagg gcctgggcaa gacgaccggc tggatacacc gggcgtcgct
     2161 caagatgaag cgcgtggaga tggtcggcga cgtccgctac gagcgcgtcg acgccgatgg
     2221 cctgcacgtc agtttcggcg aggcacgcga gcgtccgacc gtgttcgccg tggacacggt
     2281 ggtgctgtgc gccggccagg agtcggtgcg tgacctcgag gcgccgttgc gtgcggccgg
     2341 ccggcgcgtg cacctggtcg gcggcgccga ggtcgcggcc gaactcgacg cccagcgcgc
     2401 gatcgaccag gcctcgcgac tggcggcgcg gctctaggcc ggggccgtcg ctacgcggct
     2461 tcgcgcaacg cggcgccatt gccggcgcgc gccgtcaggt gcaggccgat gcccatcagc
     2521 gccatcgccc agacgatcac cgggctcgac ggcaggtccg acacgagcga cacgccgagg
     2581 ccgaccacgt atgcggcaaa cgcgagcgcg tagccgagcc agagctgccg gccgcgcgcg
     2641 tgccggtaga ccgcgagcgc gggcacgatc agcgtggtga acacgaggta gacgcctacg
     2701 agctgcaccg acgtcgtcac catgatcgag aacgcgacgt agaaaccgac cgtgccgagc
     2761 gaggcgcgga agcgaaacca gaccgcgagg aacacggcgg tcaccgccgc tgcccgtgcg
     2821 agttgttcgc tcgagaccca gaggatctg
//
LOCUS       DASPFR010000140         4393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_285426, whole genome shotgun sequence.
ACCESSION   DASPFR010000140 DASPFR010000000
VERSION     DASPFR010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285426"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..672)
                     /locus_tag="VD737_03520"
     CDS             complement(145..672)
                     /locus_tag="VD737_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HXW09656.1"
                     /translation="MAIRPYRGIHPSLGPGAWVDETAQVIGRVTLGADASVWPFVLIR
                     GDVNEIRIGARSNVQDASIVHVSRPHPGNDAGWPTLIGEDVVIGHRVALHGCTIHDRV
                     LVGIGSIVLDGVVVESDVIIGAGSVVTPGKRLESGFLYVGSPARRSRELTPQERARIP
                     KMASDYVLLKNDYLK"
     gene            792..1364
                     /locus_tag="VD737_03525"
     CDS             792..1364
                     /locus_tag="VD737_03525"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HXW09657.1"
                     /translation="MIVVTLLFLALLVVAAVYVVRVYNGLVALRENVRKAWANIDVLL
                     TQRHDELPKLVDTCKRYMRYEQETLERVMQARSAVYAAQGRGDVPALGAAEDQLRQGL
                     GRLFAVAENYPELKADQGFRHLETRISQLEEAIADRRELYNESVNLNNIRVQTFPDVI
                     VARMFDFKQSSLLEFSEEQKRDVNVGALFG"
     gene            1357..2268
                     /locus_tag="VD737_03530"
     CDS             1357..2268
                     /locus_tag="VD737_03530"
                     /inference="COORDINATES: protein motif:HMM:NF023899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HXW09658.1"
                     /translation="MADDDLWFALLTLAVAVLAGGAAALRSIRIARLIEDTPTSRVRS
                     AAQGYVELAGRALPLPDTRNLAPLTQRPCVWWRYRISKKVESGSGKNRRQRWQTVASG
                     RSSVPFLLDDDTGTCIVKPDGAEVVTTETTTWYGSTPWPAAGGPGSRSAAQWLMSSGR
                     DYRYAEERIYEHEKIYALGDFRSSASSVERDLGAEQAALLSEWKQDQPALLQRFDADG
                     DGRIDLVEWEAAREAARRTVIERQVDRPIHQNLHALCKPEGRLFLIAALPPGDLAKRY
                     RRRALWSFVGFVAAVYALGWLIQDTFG"
     gene            2289..4301
                     /locus_tag="VD737_03535"
     CDS             2289..4301
                     /locus_tag="VD737_03535"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HXW09659.1"
                     /translation="MDNPLLHEGALPAFQSIRAEYVESAIREVLDENRQRVQALLDSG
                     ASGWDGIVAPIERMQHRLARTWSPVGHLNGVVNSDELRAAYNVCLPLLTAYHTEFAQN
                     ERLYAAYRHVAETEYARLRPEQRRLLDNALRDFRLAGVALDPERKQRFKTLMERLASA
                     QAKFDENVLDATNAWMRPVTDDAELAGLPEAVKARARAEAQKAGRDGWLFMLDAPNYQ
                     AVLTHAGNRSLRRDFYTAWVTRASEQGDARWDNTALMEEILALRHEAAQLVGFASFAE
                     YSLATKMAEDPREVLEFLRRLARHCKPAAEREFAELERFAGTKLEAWDVAFHSERLKR
                     ETLAVSEEALRPYFPLPRVLEGLFAVAERLYGIRIARREGVETYHPDVDYYDILDADQ
                     ERRGGFFLDLYARPKKRGGAWMDECVGRIAVADASALPVAYLVCNFTPPATPRPSLLT
                     HHEVLTMFHEFGHGLHHMLTRVDFPSVAGINGVPWDAVELPSQFMENFAWREEVLPLI
                     SGHVETGEPLPADELRKLQATRSFQAGMQSVRQLEFALFDFRIHAEYSPDRGVRLAET
                     LAEVREEVAVVQPPAFNRFAHSFQHVFSGGYAAGYYSYKWAEVLSADAFGAFEESGIF
                     DATTARRFLQSILERGGSRDAMEAFVEFRGRKPELEPLLRQLGLAA"
BASE COUNT          705 a         1543 c         1478 g          667 t
ORIGIN      
        1 ctccgatttt tcgggctgtg ttttccggca aattccgtcg accggcccgt cacccctgaa
       61 aatccgcgct cggaagtgcc tgttttttcg ggcccagcgc gataaacagg agtgtcccca
      121 tttagccgga aattcggagt gtccctattt caggtagtcg ttcttgagga gcacgtagtc
      181 gctcgccatc ttcgggatgc gcgcgcgctc ctgcggcgtg agttcgcgac tccgccgcgc
      241 cggactgccg acgtacagga accctgactc cagccgcttg ccgggcgtga cgacgctgcc
      301 cgcgccgatg atcacgtcgc tctcgaccac gacgccgtcg agcacgatcg agccgatgcc
      361 gaccagcacg cggtcgtgga tcgtgcagcc gtgcagcgcg accctgtgcc cgatcacgac
      421 gtcctcgccg atcagcgtcg gccagccggc gtcgttgccc gggtgcggcc gcgagacgtg
      481 gacgatcgag gcgtcctgta cgttcgagcg cgcgccgatc cggatctcgt tgacgtcgcc
      541 gcggatcagc acgaacggcc acacggaagc gtccgcgccg agcgtgacgc ggccgatcac
      601 ctgcgccgtc tcgtcgaccc aggctccggg cccgagactg ggatggatgc cacgataggg
      661 tcggatcgcc atgacgaagc acacgttttc agggccgcga tgggccgcca gtgtagacgc
      721 tgcgccccgc cgggcggcaa gcttcgcgtc cccgggccct ggacccgtgc cgaccacact
      781 gggagccgtc gatgatcgtg gtgactctgc tgtttctcgc actgctcgtc gtcgcggcgg
      841 tgtacgtcgt gcgcgtctac aacggtctcg tggcgctgcg cgagaacgtg cgcaaggcct
      901 gggccaacat cgacgtgctg ctcacgcagc gccacgacga actgccgaag ctcgtcgaca
      961 cctgcaagcg ctacatgcgc tacgagcagg agacgctcga gcgcgtcatg caggcgcgct
     1021 ctgccgtgta cgcggcgcag ggccgcggcg acgtccccgc gctcggcgcc gccgaggacc
     1081 agctgcgaca gggcctcggc cggctgttcg cggtcgccga gaactacccc gagctcaagg
     1141 ccgaccaggg cttccgtcac ctggaaaccc gcatcagcca gctcgaggaa gcgatcgccg
     1201 accgccgcga gctctacaac gagtccgtga acctgaacaa catccgcgtg cagaccttcc
     1261 ccgacgtgat cgtcgcccgc atgttcgact tcaagcagtc ctcgctgctc gagttcagcg
     1321 aagagcagaa gcgcgacgtg aacgtgggcg cgctgtttgg ctgacgacga tctctggttc
     1381 gccctgctga cgctcgcggt ggccgtcctg gcgggcggcg cggcggcgtt gcgctcgatc
     1441 cggatcgcga ggctgatcga ggacacgccg acctcgcgcg tcaggtcggc cgcgcagggc
     1501 tacgtcgaac tcgccggtcg ggcgctgcca ctgccggata ccaggaacct cgcgccgctc
     1561 acacagcgac cgtgcgtctg gtggcgctac cggatctcga agaaggtcga gagcggcagc
     1621 ggcaagaacc gccgccagcg ctggcagacg gtcgcctccg gtcgctcctc ggtgccgttc
     1681 ctgctcgacg acgacaccgg cacctgcatc gtcaagcccg acggcgccga agtcgtcacg
     1741 accgagacca cgacctggta tggcagcaca ccgtggccag cggcgggtgg cccgggcagc
     1801 cgcagcgccg cgcagtggct gatgtcgagc ggtcgcgact atcgctacgc cgaggaacgc
     1861 atctacgagc acgagaagat ctacgcgctc ggcgacttcc gctcgagcgc gtcgagtgtc
     1921 gagcgcgacc tcggcgccga gcaggcggcg ttgctgtccg aatggaaaca ggaccagcct
     1981 gcgctgctcc agcggttcga cgcggacggc gacggacgga tcgacctggt cgaatgggaa
     2041 gccgcccgcg aagccgcccg ccgcaccgtc atcgaacgcc aggtcgatcg accgatccat
     2101 caaaacctgc acgcgctgtg caaaccggaa ggccgcctgt tcctgatcgc cgcgcttccc
     2161 ccgggcgacc tggcgaagcg ctaccgaagg cgcgcgctgt ggtccttcgt cggcttcgtg
     2221 gccgcggtgt atgcgctcgg ctggctgatc caggacacct tcggctgacc gggccgtaga
     2281 atgcggggat ggacaacccg ctgctgcacg aaggggccct gcccgccttc cagtcgatcc
     2341 gcgccgaata cgtcgagtcg gcgattcgcg aggtgctcga cgagaatcgc cagcgggtgc
     2401 aggcgctgct cgactcgggc gcctccggct gggacggcat cgtcgccccg atcgagcgca
     2461 tgcagcatcg gctggcccgg acctggtcgc cggtcggcca cctgaacggc gtcgtcaact
     2521 ccgacgagct gcgcgcggcc tacaacgtct gcctgccgct gctcacggcc taccacaccg
     2581 aattcgccca gaacgagcgc ctgtacgcgg cctaccggca cgtcgccgaa accgagtacg
     2641 cgcgtctgcg acccgagcaa cgacgcctgc tcgacaacgc cttgcgcgac ttccgcctcg
     2701 cgggcgtggc gctcgacccc gaacgcaagc agcgcttcaa gacgctgatg gaacggctcg
     2761 cgagcgcaca ggcgaagttc gacgaaaacg tgctcgacgc gaccaacgcc tggatgcgcc
     2821 cggtcaccga cgacgccgaa ctcgcgggac tgccggaggc ggtcaaggcc cgcgcccgcg
     2881 ccgaggcgca gaaagccggc cgtgacggct ggctgttcat gctcgacgca ccgaactacc
     2941 aggccgtgct cacgcacgcc gggaaccgct cgttgcgccg tgacttctac acggcctggg
     3001 tcacgcgcgc ctcggagcag ggcgacgcga ggtgggacaa cacggcgctg atggaggaga
     3061 tcctggcgct gcgacacgag gccgcgcagc tcgtcggatt cgcgagcttc gccgagtatt
     3121 cgctcgcgac caagatggcg gaagacccgc gcgaggtgct cgagtttctg cggcggctgg
     3181 cacggcactg caagccggcc gccgagcgcg agttcgccga gctcgaaaga ttcgccggca
     3241 cgaagctcga ggcctgggac gtcgccttcc actcggagcg tctcaagcgg gagacgctcg
     3301 cggtgtcgga ggaagcgctg aggccctact ttccgctgcc gcgcgtgctc gaaggcctgt
     3361 tcgccgtggc cgagcgactg tacggcatcc ggatcgcgcg acgcgagggc gtcgagacct
     3421 atcaccccga cgtcgactac tacgacatcc tggatgccga ccaggaacga cggggcggct
     3481 tcttcctcga cctctacgcg cggccgaaga agcgcggcgg cgcctggatg gacgagtgcg
     3541 tcggccgcat tgcggtcgcc gacgccagcg cgctgccggt cgcgtacctc gtctgcaact
     3601 tcacgcctcc cgccacgcca cgtccgtcgc tgctgacgca ccacgaggtg ctgacgatgt
     3661 ttcacgagtt cggccacggg ctgcaccaca tgctgacgcg cgtcgacttc ccgagcgtcg
     3721 ccggcatcaa cggcgtgccc tgggacgcgg tcgaactgcc cagccagttc atggagaact
     3781 tcgcctggcg cgaggaagtg ctgccgctga tctccgggca cgtcgaaacc ggcgagccgc
     3841 tgccggcgga cgaactccgc aagctgcagg ccacgcgctc gttccaggcc ggcatgcagt
     3901 cggtgcgcca gctcgagttc gcgctgttcg acttccggat ccacgccgag tattcgcccg
     3961 accggggcgt ccggctggcg gagacgctgg cggaagtgcg ggaggaagtc gccgtcgtgc
     4021 agccgccggc gttcaaccgg ttcgcgcaca gcttccagca cgtcttctcc ggcgggtacg
     4081 ccgccggcta ctacagctac aagtgggccg aggtgctgtc cgccgacgcg ttcggcgcgt
     4141 tcgaggagtc cggcattttc gacgccacga cggcgcgacg cttcctgcag tcgatcctcg
     4201 agcgcggcgg cagccgtgac gcgatggagg cgttcgtcga gttcaggggc cgcaagcccg
     4261 agctcgagcc gttgctccgc cagctgggac tggccgcgta gcgcgcagcg gtcccctggg
     4321 gccacgaccg tgtcgaagcg ctgctggccc tgtcgttcga ccgcgagggc gacgacgcgc
     4381 cctagaaacg ccg
//
LOCUS       DASPFR010000141         6660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_41504, whole genome shotgun sequence.
ACCESSION   DASPFR010000141 DASPFR010000000
VERSION     DASPFR010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41504"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="VD737_03540"
     CDS             <1..1064
                     /locus_tag="VD737_03540"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HXW09660.1"
                     /translation="LKRYLEQDYLPKSRDTVGLWDTPNGTQRYAELARFYTTTDLTPD
                     QIHELGLKEVARIRAEMQQVIAKTGFEGSFEEFLVYLRTDPKFHYTDPKQLLVAYEAM
                     AKRVDPLLPQYFGKLPRMPYGVRPIAEQIAPDTTTAYYQSPALDGSRAGYYYVNLYKP
                     EERPRYEIPVLTIHEAVPGHHLQISLAQELGELPKFRRDFEATAFVEGWALYAESLGQ
                     EMGFYDDPYDKFGQLTYEMWRAVRLVVDTGLHHKRWTRQQAIDYFKANAAKSELDIVN
                     EIDRYIGNPGQALAYKIGELRIKELRREASDRLGERFDLRAFHDVVLGSGAIPLDVLS
                     ANVRRWVEATRAAPTAAGR"
     gene            1095..2363
                     /gene="ispG"
                     /locus_tag="VD737_03545"
                     /gene_synonym="gcpE"
     CDS             1095..2363
                     /gene="ispG"
                     /locus_tag="VD737_03545"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HXW09661.1"
                     /translation="MSNAIVRRPSTAVRIGPVTVGGGAPIVVQSMTNTDTADVEATAL
                     QVRALAIAGSELVRITVNTEEAAAAVPRIVERLAQLGTSVPIVGDFHFNGHKLLREHP
                     GCAEALAKYRINPGNVGRGSKRDPQFAEMIEAACRHGKAVRIGVNWGSLDQDLLTRLM
                     DENSRRPQPLDATDVMREAIVVSALESARRAEELGLPHDRIVISCKVSGVQDLIAVYR
                     SIARRCDYALHLGLTEAGMGSKGIVASTAGIGVLLQEGIGDTIRVSLTPEPGGDRTNE
                     VIVAQEILQTMGLRSFTPMVVACPGCGRTTSTYFQELAQQIQSHLRRRMPEWRRRYAG
                     VEQMTVAVMGCVVNGPGESKHANLGISLPGTGERPVAPVYIDGEKGPTLKGERIAEEF
                     QQLVEEYVERTYGGQLHARMEETVSHDGLA"
     gene            2347..2676
                     /locus_tag="VD737_03550"
     CDS             2347..2676
                     /locus_tag="VD737_03550"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HXW09662.1"
                     /translation="MTALRDRHCRPLPAGTPALAPEQAVELLAQLDAGWRISEDGKSL
                     RRAFGFKDFHRTMGFLNAVAWIANAEDHHPDLEAGYDYCRLAFSTHSIGGLSENDFVC
                     AAKIDAL"
     gene            2700..3686
                     /locus_tag="VD737_03555"
     CDS             2700..3686
                     /locus_tag="VD737_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HXW09663.1"
                     /translation="MRYVAATAAGGPDVLEVATTSVPVPAADEVLVRVHAAGVNRPDV
                     MQRKGLDPPPPGASPILGLEVAGEVVGVGADVRNVAVGEQVCALTNGGGYAEYCTVPS
                     LQCLPWPRGFDAIRAAALPETFFTVWANLMLHGRLARGESVLVHGGTSGIGTVAIQLA
                     RAFEARVYATAGSDAKCAACLGLGAEAAINYRTEDFGARIAELTGGRGVDVILDIVGA
                     PYFERNLASLAMDGRLVEVATQQGAVAERLDLFLVMRKRLTITGSTMRPRTTAQKGAI
                     AGALRREAWPLLDTGRVAPVIHAVYPFEQAAEAHRVLESSVHVGKLVLSFPP"
     gene            complement(3705..4451)
                     /locus_tag="VD737_03560"
     CDS             complement(3705..4451)
                     /locus_tag="VD737_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09664.1"
                     /translation="MCPNPTPNTPTSRTARSAAEPSRRRAARALAPGAARCRRRFLRF
                     FPGGFRDETYLDWERGYKWETHERWNAALGREAFERLLERGGHDEVAARAVRVEQQSR
                     HSMIFSFEKMALRDAIRSPAGARAFANGLYQFLHGSGTDEDRFARWCDVVAGLPRRQT
                     RVLTWPLVTVFGFIAQPDRHVFLKPLVTRAAAVKYGFDFSYRPRPNWETYRSLLDFAA
                     LVRREQRDLRPRDMIDLQSFIWVQGSDEYA"
     gene            complement(4393..4644)
                     /locus_tag="VD737_03565"
     CDS             complement(4393..4644)
                     /locus_tag="VD737_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09665.1"
                     /translation="MDPVSIERLRSREYQLHAYCPVCDRWKLLNLDEMLDRWRGLDRA
                     PTEVQCAECGHFGLLRVRNRVVPQPDAEHADVPDRALGG"
     gene            5088..5552
                     /locus_tag="VD737_03570"
     CDS             5088..5552
                     /locus_tag="VD737_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HXW09666.1"
                     /translation="MALAGSRTGQYLIALAATAAAVLLRAVLDPWLGDAGPFSTLYGA
                     VAVATWVGGLGAAVLAALSGLVVSEAWFAPRPTSLAAVITYAVSCSIIIALGHEARRR
                     IARARRRRGTAQARERDERPGAAGARREPRKLQPARRCDAAAGLDGASRRRR"
     gene            5509..>6660
                     /locus_tag="VD737_03575"
     CDS             5509..>6660
                     /locus_tag="VD737_03575"
                     /inference="COORDINATES: protein motif:HMM:NF020036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXW09667.1"
                     /translation="MPQLVWTARADGVVDYYSARRAAYHGAHAPPDEWHCMVHPDDLE
                     ATRDAWHRASSTGVPYDIEHRVLMADGTYRWHLSRALPVFDHDGRIARWYGTATDIDR
                     LKRAEGELALTSASLRRERERLAVALRAGRMGIYEWQTGTSQVWWSRELYDVFGIDPA
                     SFVPTLEACTELVVPEDRAELWRRTEDCLERRGELTHQYRVCRPDGQVRWLQIQARVT
                     AAPDGEPAYLTGVAFDVTERRRAEEQLLEADRRKDRFIATLAHELRNPLAPIRNAVRL
                     LAPDVQGRGDPTWARDVIERQVSQMARLLDDLLDISRISRDRVELQVGRVTLASVVDL
                     AVETSQPAIERSRHELVVSVPPEPIWLEADAVRLAQVLSNLLNNAAKYTE"
BASE COUNT         1044 a         2353 c         2314 g          949 t
ORIGIN      
        1 gcctcaagcg ttacctcgag caggattacc tgccgaagtc gcgcgacacg gtcggcctct
       61 gggacacgcc caacggtacg cagcgttacg ccgagctggc caggttctac acgaccacgg
      121 acctgacgcc cgaccagatc cacgaactcg gcctgaagga ggtcgcgcgg atccgcgccg
      181 agatgcagca ggtgatcgcg aaaacgggct tcgagggcag cttcgaggaa ttcctggttt
      241 acctgcgcac cgatccgaag ttccactaca ccgatccgaa gcagctgctc gtcgcctacg
      301 aggccatggc caagcgggtc gatccgctgc tgccgcagta cttcggcaag ctgccccgca
      361 tgccgtacgg cgtgcgcccc atcgccgagc agatcgcgcc cgacacgacg accgcgtact
      421 accagagccc cgcgctcgac ggcagccgag ccggctatta ctacgtgaac ctctacaagc
      481 ccgaggagcg ccccaggtac gagatcccgg tgctgacgat ccacgaggcg gtgccggggc
      541 atcacctgca gatctcgctg gcgcaggaac tcggcgagct gccgaagttc cgccgtgact
      601 tcgaggcgac ggcgttcgtc gagggctggg cgctgtacgc ggaaagcctc ggccaggaaa
      661 tgggcttcta cgacgacccg tacgacaagt tcggccagct tacctacgag atgtggcgcg
      721 cggtgcgcct cgtcgtggac accgggctgc accacaagcg ctggacccgc cagcaggcca
      781 tcgactactt caaggcgaac gcggcgaaga gcgaactcga catcgtcaac gagatcgacc
      841 gctacatcgg caatccgggc caggcgctcg cctacaagat cggcgagctg cgcatcaagg
      901 agctgcgacg cgaggcgagc gatcggctcg gggagcgctt cgacctccgc gcgttccacg
      961 acgtggtgct cggcagcggc gcgataccgc tcgacgtgct gtcggccaac gtccgccgct
     1021 gggtcgaggc cacccgggcg gcgccgacgg cggccggccg gtagaattgg cgtttcgcgc
     1081 cgcgcgccct tcccatgagc aacgccatcg tccgccgccc cagtactgcc gtcaggatcg
     1141 gcccggtcac ggtcggcggc ggcgcgccga tcgtcgtcca gtcgatgacc aataccgaca
     1201 cggccgacgt cgaggcgacg gcgctgcagg tgcgtgcgct cgcgatcgcc ggctccgagc
     1261 tggtccgcat cacggtcaac accgaggagg cagcggccgc ggttccgcgg atcgtcgagc
     1321 ggctcgcgca gctcggcacg agcgtgccga tcgtcggcga cttccacttc aacgggcaca
     1381 agctgctgcg cgagcacccg ggctgcgccg aggcactcgc caagtaccgg atcaaccccg
     1441 gcaacgtcgg ccgcggcagc aagcgcgacc cgcagttcgc cgagatgatc gaagcggcct
     1501 gccgtcacgg gaaggcggtg cgcatcggcg tcaactgggg cagcctcgac caggacctgc
     1561 tcacgcgcct gatggacgag aactcgcgtc ggccgcagcc gctcgacgcg accgacgtga
     1621 tgcgcgaggc gatcgtcgtc tcggcgctgg agagcgcgcg gcgcgccgag gaactcggcc
     1681 tgccgcacga ccgcatcgtg atctcgtgca aggtgagcgg cgtgcaggac ctgatcgcgg
     1741 tctatcgcag catcgcgaga aggtgcgatt acgcgctgca cctcggcctg accgaggccg
     1801 gcatgggctc gaagggcatc gtcgcctcga ccgccggcat cggggtcctg ctgcaggaag
     1861 gcatcggcga caccatccgc gtctcgctca cgcccgagcc gggcggcgac cgcaccaacg
     1921 aagtcatcgt cgcgcaggag atcctgcaga cgatgggcct gcggtcgttc acgccgatgg
     1981 tcgtggcgtg cccgggctgc ggccgcacca cctcgaccta cttccaggaa ctcgcgcagc
     2041 agatccagtc gcacctgcgg cggcgcatgc cggagtggcg gcgccggtat gcgggcgtcg
     2101 agcagatgac ggttgcggtc atgggttgcg tcgtcaacgg ccccggcgag agcaagcacg
     2161 ccaacctcgg catcagcctg cccggcacgg gcgagcgtcc ggtcgcaccg gtctacatcg
     2221 acggcgagaa ggggccgacg ctcaaggggg agcggatcgc cgaggagttc cagcagctgg
     2281 tcgaggaata cgtcgagcgc acgtacggcg gccagcttca tgcccggatg gaggagacgg
     2341 tgagccatga cggccttgcg tgatcgacat tgccggccgc tgccggcagg cactcccgcg
     2401 ctggcgcccg agcaagccgt cgagctgctg gcacagctcg acgcgggctg gcgcatttcc
     2461 gaggacggca agtcgctgcg acgcgcgttc ggattcaagg acttccaccg cacgatgggg
     2521 ttcctgaacg ccgtggcctg gatcgcgaac gccgaggatc accatccgga cctcgaggcg
     2581 ggctacgact attgccgact cgcgttctcg acgcactcga tcggcgggtt gtccgagaac
     2641 gacttcgtct gcgccgcgaa gatcgacgcg ctttgagcgc cgtgcgggtc ccgtcgacga
     2701 tgcgttacgt cgcggcgacc gcggccggcg gccccgacgt gctcgaggtc gcgacgacat
     2761 ccgtgccagt tcccgcggcg gacgaagtgc tggtccgggt gcacgcggcc ggcgtgaacc
     2821 ggcccgacgt gatgcagcgc aagggcctgg acccgccccc acccggcgcg agccccatcc
     2881 tgggcctcga ggtcgcgggc gaggtcgtcg gggtcggcgc cgacgtgagg aacgtggcgg
     2941 tcggcgagca ggtctgcgcc ctcacgaacg gcggcggcta cgccgagtac tgcaccgttc
     3001 cctccttgca atgcctgccg tggccgcgcg gtttcgacgc catccgcgcc gcagccctgc
     3061 ccgagacgtt cttcacggtg tgggccaacc tgatgctgca cggccggctc gcacgcggcg
     3121 agtcggtgct cgtccacggc ggcaccagcg gcatcggtac cgtcgcgatc cagctggcgc
     3181 gggccttcga ggcgcgcgtc tatgcgacgg ccgggagcga tgcgaagtgc gcggcgtgcc
     3241 tgggcctcgg cgcggaggcg gccatcaact accgcaccga ggacttcggg gcgcggatcg
     3301 ccgaattgac gggcggccgc ggcgtggacg tgatcctcga catcgtcggc gcgccctact
     3361 tcgagcgcaa cctcgcgtcg ctcgccatgg acggacggct ggtcgaggtc gcgacgcagc
     3421 agggcgcggt cgccgagcga ctcgacctgt tcctggtgat gcgcaagcgc ctgacgatca
     3481 ccggctcgac gatgcggcct cgcacgacgg cgcagaaggg cgccatcgcc ggggcactgc
     3541 gccgcgaagc ctggccgctg ctcgacaccg ggcgcgtcgc gccggtgatc cacgcggtgt
     3601 atccgttcga gcaggccgcc gaggcgcacc gcgtgctcga gtcgagcgtg cacgtcggca
     3661 agctggtgct gagcttcccg ccgtagctcg gcgcaggagg cccgtcaggc gtactcgtcg
     3721 gaaccctgca cccagatgaa cgactgcagg tcgatcatgt cgcgcggtcg caggtcgcgc
     3781 tgctcgcggc ggacgagcgc cgcgaagtcg agcaggctgc ggtaggtctc ccagttgggt
     3841 cgcgggcggt aactgaagtc gaacccgtac ttgaccgcgg cagcccgcgt gacgagcggc
     3901 ttcaggaaga cgtggcggtc gggctgtgcg atgaagccga acacggtcac cagcggccag
     3961 gtcagcacgc gcgtctggcg gcgcggcagg ccggccacca cgtcgcacca gcgcgcgaac
     4021 cggtcttcgt ccgtaccgga gccgtgcagg aactggtaga ggccgttcgc gaacgcgcga
     4081 gcgccggcgg gcgaccggat cgcatctcgc agcgccatct tctcgaacga gaagatcatg
     4141 ctgtggcggg attgctgctc cacgcggacc gcgcgcgcgg cgacctcgtc gtgcccgccg
     4201 cgttcgagca ggcgctcgaa ggcttcgcga ccgagcgccg cgttccagcg ctcgtgcgtc
     4261 tcccatttgt atccgcgctc ccagtcgagg taggtctcgt cgcggaaccc gccgggaaag
     4321 aaccgcagga acctgcgccg acacctggcc gcaccaggcg cgagggcacg ggccgcccgt
     4381 cttcgggacg gctcagccgc cgagcgcgcg gtccgggacg tcggcgtgtt cggcgtcggg
     4441 ttggggcaca actcggtttc tcacccgcag cagtccgaag tgtccgcact ccgcgcactg
     4501 cacttcagtc ggcgcccggt cgagcccgcg ccaccggtcc agcatctcgt cgaggttgag
     4561 gagtttccag cgatcgcaaa cggggcagta ggcatgcagc tggtactcgc ggctgcggag
     4621 ccgctcgatc gacaccggat ccatgagcgg accataccga agctcaccgc tccgctgtac
     4681 gcgtccgacc ccgaatgaat cgtgggattt gccgcacgcg tcccggcgtt ccgcgtctcg
     4741 cgccatcgct gcacgaggct cgatcggcca ggcggccgtc ccgacccagc cgggattggt
     4801 ccagaaagtt gtgctcgctt gtagtcatcg tctgactttc gtgtccgcgt gcgcccggca
     4861 gaatgtgccg ctcatcaggg aggactccac tcatgaagcg gcgctgcccc cactgccaga
     4921 cgaccccgtt gcgtgcggac gcggacacct gctggtattg cgggaaggac tatgcgccgc
     4981 ggcgccagct ccgccgtggc gccccggcac cgaccgcttc cctgcgaaga cgacgaaccg
     5041 actcgagggc gtccaccccc gcggcggccc gatgacctga tccggccgtg gcactcgcgg
     5101 gctcacggac cggccagtac ctgatcgccc ttgccgcgac cgcggcggcc gtgctgctgc
     5161 gcgcggtgct cgacccctgg ctcggcgacg ctggcccgtt ttcgacgctc tacggcgcgg
     5221 tcgcggtcgc gacctgggtc ggcggcctcg gggcggccgt cctggcggcg ctctccgggc
     5281 tggtcgtcag cgaagcatgg tttgcgcccc gcccgaccag cctggcggcc gtgatcacgt
     5341 acgcagtctc ctgctcgatc atcatcgcgc ttggacacga ggcgcgccgt cgcatagcgc
     5401 gcgcgcggcg acgccgcgga acggcgcagg ctcgagagcg agacgaacga cctggcgctg
     5461 caggcgctcg ccgcgagccg cgcaaacttc aacctgctcg ccgatgcgat gccgcagctg
     5521 gtctggacgg cgcgagccga cggcgtcgtt gattactaca gcgcgcgccg tgcggcgtac
     5581 cacggcgcgc acgccccgcc cgacgaatgg cactgcatgg tgcatccgga cgacctcgag
     5641 gccacgcgcg acgcctggca ccgcgcgagc tcgaccggcg tgccttacga catcgagcac
     5701 cgcgtactga tggcggacgg cacgtatcgc tggcacctga gccgcgcgct gcccgtgttc
     5761 gaccacgacg gtcgcatcgc gaggtggtac ggcacggcca ccgacatcga ccgcctgaag
     5821 cgcgccgaag gcgagctcgc gttgacgtcg gcgtcgctgc gtcgcgagcg tgaacgcctc
     5881 gccgtcgcgc tgcgcgccgg tcgcatgggc atctacgagt ggcagaccgg gacgtcgcag
     5941 gtctggtggt cgcgcgaact gtacgacgtc ttcggcatcg acccggccag cttcgtcccc
     6001 acgctggagg cctgcacgga actggtcgtg cccgaggatc gcgcggaact ctggcgacgc
     6061 accgaggact gcctcgagcg tcgcggcgag ctgacgcacc agtaccgcgt ctgccgcccg
     6121 gacggccagg tgcgctggct gcagatccag gcgcgcgtga cggccgctcc cgacggcgag
     6181 ccggcctacc tgaccggcgt ggcgttcgac gtcaccgagc gcaggcgcgc cgaggaacag
     6241 ttgctcgaag cggaccgccg caaggaccgt ttcatcgcga cactcgcgca cgaactgcgc
     6301 aacccgctcg ccccgatccg caacgccgtg cgactgctcg caccggacgt ccagggccgc
     6361 ggcgacccca cctgggcgcg cgacgtgatc gagcgccagg tttcgcagat ggcccggcta
     6421 ctggacgatc tcctcgacat cagccggatc agccgcgatc gcgtcgagct gcaggtcgga
     6481 cgcgtgacgc tcgcgtccgt ggtggacctg gccgtggaaa cgagtcagcc cgccatcgag
     6541 cgcagccgcc acgagctcgt cgtctcggtc ccgcccgagc cgatctggct cgaggccgac
     6601 gccgtgcgcc tggcccaggt gctgtccaac ctgctgaaca acgcggccaa gtacacggag
//
LOCUS       DASPFR010000142         3500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1750667, whole genome shotgun sequence.
ACCESSION   DASPFR010000142 DASPFR010000000
VERSION     DASPFR010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1750667"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1518)
                     /locus_tag="VD737_03580"
     CDS             complement(<1..1518)
                     /locus_tag="VD737_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HXW09668.1"
                     /translation="MTNVLRPERLREFHRLLGERVLVLDGAMGTVIQSYRLDESDYRG
                     DRFRDHAHDLKGNNDLLVLTRPEVIGEIHRRYLDAGADIVETNTFNSNAMSQADYGTE
                     SLVREINLAAAQVARRAADEVAARTGRPRFVAGALGPTNRTASLSPDVNDPGRRNVTF
                     DALAATYADATRALVEGGADLILVETIFDTLNAKAALYAVRQVFDELGVELPIMISGT
                     ITDASGRTLSGQTVEAFWNSVRHARPAVIGLNCALGAKQLRPYVEELARIADTAVCAY
                     PNAGLPNAFGEYDETACETAALVRDYVESGFVNIVGGCCGTTPEHIEHVAEAIRGLPP
                     RRPPAVPVRCRLSGLEPLNVGPESLFVNVGERTNVTGSAKFRRLIEADDYAGALEVAR
                     QQVASGAQVIDVNMDEGMLDSEAAMVRFLNLVAVEPDVARVPVMIDSSKWSVIEAGLK
                     CLQGKGIVNSISLKEGERPFLAQARKVRAYGAAVVVMAFDEQGQADTVERKVGICQ"
     gene            complement(1515..2450)
                     /gene="metF"
                     /locus_tag="VD737_03585"
     CDS             complement(1515..2450)
                     /gene="metF"
                     /locus_tag="VD737_03585"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF006950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HXW09669.1"
                     /translation="MTAASAATRVPPSPAPGGRRPVEVSFEFFPPNDAAMEQTLWASL
                     QRLAPLGPRFVSVTYGADGSTRERTHNVVTRILRETALTAAPHLTCIGAPRGEILDIA
                     RSYWDAGVRHVVALRGDAPAGQGGHAPRRDGFAYAVDLVRGLRTVGDFEISVAAYPET
                     HPEAPNAQFDLDNLRAKIDAGATRAITQFFFDPDLYLRFRDRCEAAGIRARIVPGILP
                     ITRFPHMLKFARRCGASVPEWLVQRFDGLDDDPDTRRMIAASCAIEQVRRLQEHGVHE
                     FHFYTLNRAELTYAICHALGVRAPAPLAGPQAVQA"
     gene            complement(2474..3361)
                     /locus_tag="VD737_03590"
     CDS             complement(2474..3361)
                     /locus_tag="VD737_03590"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXW09670.1"
                     /translation="MEALRAAAEPSRLRLLWVLARGEFSVTELTRIVGQSQPRVSRHL
                     KLLGDAGLLEKFREQHWIYYRVPADGAGRDFVDAVLARIDVSDPVLQADRARVESVLA
                     DRQVTAQRDDDSGPPTEPVELVAAIAEALGDRGRGALFYFGAAPGPVLESIASRARRV
                     VGMHGSRPGLQRARALLHARGLSHCVLQQGALERLPHASGEFETVVLDRVLSAQARPG
                     DALREAARLLDPRGELIVAEDYEALAARSAGSHPLATLKAWLAEAGLLCVRLHPVDLD
                     GRHVVLAVAQPGAAVAAAA"
BASE COUNT          524 a         1226 c         1239 g          511 t
ORIGIN      
        1 ctggcagatc ccgaccttgc gctcgacggt gtcggcctgg ccctgctcgt cgaacgccat
       61 cacgacgacc gccgcgccgt acgcgcgcac cttgcgcgcc tgcgcgagga acggacgctc
      121 gccttccttg aggctgatcg aattgacgat gcccttgccc tgcaggcact tgagtcccgc
      181 ctcgatcacg gaccacttcg acgagtcgat catgacggga acgcgcgcga cgtcgggctc
      241 gacggccacc aggttcagga agcgcaccat cgccgcttcc gagtcgagca tgccctcgtc
      301 catgttgacg tcgatgacct gcgcgccgct cgcgacctgc tgccgcgcga cctcgagcgc
      361 gcccgcgtag tcgtccgcct cgatcaggcg gcggaacttg gccgagccgg tcacgttggt
      421 ccgctcgccc acgttcacga acaggctctc ggggccgacg ttgagcggct cgaggccgct
      481 caggcggcaa cggaccggca ccgccggcgg acgacgcggc ggcaagccgc ggatggcctc
      541 ggcaacgtgc tcgatgtgct ccggggtggt gccgcagcag ccgcccacga tgttgacgaa
      601 gccgctctcc acatagtcgc gcacgagcgc cgccgtctcg caggccgttt cgtcgtactc
      661 gccgaacgcg ttcggcagcc ctgcgttcgg gtacgcacag acggcggtgt cggcgatccg
      721 cgccagttcc tcgacgtacg gccgcagttg cttcgcgccg agcgcgcagt tgagcccgat
      781 cacggccgga cgcgcgtgcc ggaccgagtt ccagaacgcc tcgacggtct ggccggacag
      841 cgtgcgaccg gaggcgtccg tgatcgtgcc ggagatcatg atcggcagct cgacgcccag
      901 ctcgtcgaac acctggcgga ccgcgtagag cgcagccttg gcattcagcg tgtcgaagat
      961 cgtctcgacg aggatcaggt cggcaccgcc ctcgaccagg gcgcgcgtcg cgtccgcgta
     1021 cgttgccgcg agcgcgtcga acgtgacgtt gcgacggcca ggatcattga cgtccggcga
     1081 cagtgacgcc gtgcggttcg tgggccccag cgccccggcc acgaatcgcg gccggcccgt
     1141 gcgggcggcg acctcgtccg cggcgcggcg cgcgacctgg gccgccgcga ggttgatctc
     1201 gcgcacgagc gactcggtgc cgtagtcggc ctgcgacatg gcgttggagt tgaacgtgtt
     1261 ggtctcgacg atgtccgcgc ccgcgtccag gtatcgccgg tggatctcgc cgatgacctc
     1321 ggggcgtgtc agcaccagca ggtcgttgtt gcccttgagg tcgtgggcat gatcccgaaa
     1381 gcggtcgccg cggtagtcgg actcgtcgag tcgatacgac tggataacgg tgcccatcgc
     1441 cccgtcgagc acgagcacgc gctcgccgag cagccggtgg aactcgcgca ggcgttcggg
     1501 tcgcagcacg ttcgtcacgc ctgcaccgcc tgcgggcccg caagcggcgc cggggcgcgc
     1561 acgccgagcg cgtgacagat cgcgtaggtc agctcggcac ggttgagcgt gtagaagtgg
     1621 aactcgtgca cgccgtgttc ctgcaggcgg cggacctgct cgatcgcgca gctcgccgcg
     1681 atcatgcgtc gcgtgtccgg gtcgtcgtcg agcccgtcga accgctgcac gagccactcg
     1741 ggcaccgacg cgccgcagcg tcgcgcgaac ttgagcatgt gtggaaaacg cgtgatcggc
     1801 aggatgcccg gcacgatcct ggcgcggatg ccggcggcct cgcagcggtc gcgaaagcgc
     1861 aggtacaggt cggggtcgaa gaagaactga gtgatcgcgc gcgtcgcgcc cgcgtcgatc
     1921 ttcgcccgca ggttgtcgag gtcaaattgc gcgtttgggg cctccggatg cgtttccgga
     1981 taggcggcca ccgaaatctc gaaatcaccg acggtccgca gcccgcgcac gaggtcgacg
     2041 gcgtaggcga aaccgtcccg acggggcgcg tggccaccct gccccgccgg tgcgtcgcca
     2101 cgcagcgcga cgacgtgccg cacgcctgcg tcccagtagg agcgggcgat gtcgaggatc
     2161 tcgccgcgcg gcgcgccgat gcacgtcagg tggggtgccg cggtgagcgc ggtctcgcgc
     2221 aggatgcgcg tgacgacgtt gtgcgtgcgc tcgcgggtcg agccgtcggc accgtacgtc
     2281 accgacacga acctcgggcc aagcggcgcc agtcgctgga gcgacgccca cagcgtctgt
     2341 tccatggcgg cgtcgttggg cgggaagaac tcgaacgaca cctcgacggg gcgtcgcccg
     2401 cccggcgccg gcgacggcgg gacgcgggtg gcagcactgg cggcggtcat ggcgtgggtc
     2461 cttgcaggag aggtcaggcg gcagccgcga cggcggcgcc gggttgcgcg acggccagca
     2521 cgacgtgccg cccgtccagg tcgaccgggt gcaggcggac gcagagcagt ccggcctcgg
     2581 ccagccaggc cttgagcgtc gcaagcggat ggctgcccgc agaccgggcc gccagcgctt
     2641 cgtagtcctc tgcgacgatc aactcgccgc gggggtcgag cagccgggcc gcttcgcgca
     2701 gcgcgtcgcc gggacgcgcc tgcgccgaca gcacccggtc gagtacgacc gtttcgaact
     2761 cgcccgacgc gtgcgggaga cgctcgagcg cgccctgctg cagcacgcag tggctgaggc
     2821 ctcgggcgtg cagcagcgcc ctcgcccgct gcagtccggg gcgcgatccg tgcatgccga
     2881 cgacgcgcct ggcacgcgat gcgatcgact cgagcaccgg gccgggcgcc gcgccgaaat
     2941 aaaagagcgc gccacggccg cgatcgccga gcgcctccgc gatcgccgcc acgagttcga
     3001 cgggctcggt cggcgggcca ctgtcgtcgt cccgttgggc ggtcacctgt cgatcggcga
     3061 gcacggattc gacacgggct cgatcggcct gcagcacggg atcggacacg tcgatccgcg
     3121 cgagcacggc gtcgacgaag tcgcgcccgg cgccgtcggc cggcacgcgg tagtagatcc
     3181 agtgctgctc gcggaatttc tcgagcaggc cggcgtcgcc gagcagtttc aggtggcgcg
     3241 aaacgcgcgg ctggctctgg ccgacgatgc gggtcaactc ggtgaccgaa aactcgcccc
     3301 gggccagcac ccacagcaga cgcagccggg acggctcggc cgcggcccgg agagcttcca
     3361 cgtgttgttc aaagcccagc cccatacagc aaatataaac atatttttat tcttgtccac
     3421 caccaggcct ttcggtctag catctcgacc caccctcccg gccggccgga gcgcacatga
     3481 ccgaatcggc gttcgcctcg
//
LOCUS       DASPFR010000143         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1751544, whole genome shotgun sequence.
ACCESSION   DASPFR010000143 DASPFR010000000
VERSION     DASPFR010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751544"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1548
                     /locus_tag="VD737_03595"
     CDS             <1..1548
                     /locus_tag="VD737_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXW09671.1"
                     /translation="MDLGYYHRTMVEPGLTRRDLLSRLAAAAALPIAASCGRGPAGSS
                     ASTTRGPLHYSSLADVATLIAARELTSLDVTQQLLARIAAADGHLQSYVTVMTDHAIA
                     SAERADTEIRAGRYRGPLHGVSIGVKDLCYTRGVRTMAGTKVFADFVPDYDATVVARL
                     EAAGAVILGKLVLCEGAFGPYFPGSQVPVNPWDATRWSGVSSSGSGVATAAGLCFASV
                     GTDTGGSIRYPSAANGCVGLKPTYGRVSRYGVFALAPSMDHVGPMTRTVEDAAIMFEA
                     MAGHDPHDTTSLPDPVSPVRAELARGIAGLRIGFDRRYATDNVDADLAEAMDEVLVTL
                     TRLGTTVVPVTMPDVSQAGGAWLDLCYVEALAVHASTFPSRAGEYGPGFRAALEAGQR
                     VTPSTLAAAARVRAEVSSSIESMLETVDCLVCPSMANSARVKEANPFDEETAESWNAT
                     VRNDIHTQPFNFSGSPTLSVPCGFSAEGLPLSVQFVGRRLSESVLCRIGHAYEQTTPW
                     HLKHPAI"
     gene            1573..2073
                     /locus_tag="VD737_03600"
     CDS             1573..2073
                     /locus_tag="VD737_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09672.1"
                     /translation="MDGDNYLYALVEAGVALAGFSAIAIALESRGVQQSETRQKAVIA
                     RLVERSLMASLFALLPTLLFGLDISERIVWLIASGSLAGYGAFRLVQTLRTHKETMVQ
                     MRVGGATRFVLGLVAVAVIIIQVLNAIGFVLSQGQWWYALGVTWLMVTAGYTFVIYLR
                     AWMRGS"
BASE COUNT          348 a          693 c          726 g          419 t
ORIGIN      
        1 atggacctgg ggtactatca ccgcaccatg gtcgaacctg ggctgacgcg ccgcgatctc
       61 ctgtcacgcc tggccgccgc tgcggcactg ccgatcgcgg cgtcgtgcgg gcgaggcccg
      121 gcgggttctt ccgcctcgac aaccaggggc ccgctgcact actcgagcct ggcagacgtg
      181 gctacgctga tcgcggcccg ggagctcacg tcgctggacg tcacgcaaca gctgctcgcc
      241 cgtatcgcgg cggcggacgg gcacctgcag agctacgtga cggtcatgac cgaccacgcg
      301 atcgcgagcg ccgagcgcgc ggacacggag attcgcgccg gccggtaccg cgggcctctg
      361 cacggcgtgt cgatcggggt gaaggacctt tgctatacgc gcggcgttcg caccatggcg
      421 ggcacaaagg tctttgccga tttcgtgccc gactacgacg ccactgtcgt tgcacgactg
      481 gaggccgccg gcgccgtgat cctcggcaag ctcgtcctgt gcgaaggtgc gttcggaccg
      541 tatttccccg gctcgcaggt gcctgtgaac ccgtgggatg cgacgcgatg gagcggcgtc
      601 tcgtcgagcg gctccggcgt ggctacggca gcaggcctgt gtttcgcttc ggtcggtacc
      661 gataccgggg ggtcgatccg gtacccgtcg gccgcgaacg gctgcgtggg cctcaagccc
      721 acgtacggcc gcgtgagccg ctacggtgtg ttcgcgctgg cgccctccat ggatcacgtc
      781 gggccgatga cacgcacggt ggaggacgcc gccatcatgt tcgaagcgat ggccggtcac
      841 gatcctcacg acacgacgtc gctgccggac ccggtttccc cggtacgtgc cgaactggcg
      901 cggggcatcg cgggcctgcg tatcgggttc gaccgtcgct atgccactga caacgtggat
      961 gccgacctgg ccgaggcgat ggacgaggtg ctcgtgacat tgacccgcct gggcacgacc
     1021 gtcgtgcccg tcaccatgcc ggatgtcagc caggccggcg gcgcgtggct cgacctgtgc
     1081 tatgtcgagg cgcttgcggt gcatgcgtcg acgtttccga gccgggccgg tgaatatggg
     1141 ccgggatttc gcgccgcgct cgaggccggt cagcgggtca cgccatcgac gctggccgcg
     1201 gccgcgcgcg ttcgtgcaga ggtctcctcg tcaatcgaat ccatgctgga aaccgtcgac
     1261 tgcctggtgt gcccgtcgat ggccaacagc gcacgcgtca aggaagcgaa tccttttgac
     1321 gaagaaaccg cagagtcctg gaatgcgacc gtccggaacg acatccacac ccagccattc
     1381 aatttcagtg gcagtcccac cttgtcggtg ccgtgcgggt tctcggcgga gggcttgccg
     1441 ctcagcgtgc agttcgtggg tcgccgactt agcgaatcgg tgctgtgccg catcggccac
     1501 gcgtacgagc agacgacccc gtggcacttg aaacatcccg ccatctaatt ggcacgtcag
     1561 ggagcaccag ttatggacgg cgacaactat ctgtacgcgc tggttgaagc gggagtggcg
     1621 ctggccggct tctctgcgat cgcgatcgcc ctggaatcca ggggcgtgca gcagagcgaa
     1681 acccgccaaa aggccgtcat cgcccgcctc gttgaacgca gtctcatggc gtccctgttt
     1741 gccctattgc caaccctgct gtttggactg gacatctcgg aacggatcgt gtggttgatt
     1801 gccagcggat cattggctgg gtacggcgcg tttcggctgg ttcaaacgtt gcgaactcac
     1861 aaagagacga tggtgcagat gagagtaggt ggggccactc gcttcgttct cggccttgtc
     1921 gccgtcgccg tcatcatcat tcaagtcctg aatgcgattg gcttcgtcct ttcgcaaggc
     1981 caatggtggt acgcgctcgg agtcacctgg ttgatggtga ctgccgggta caccttcgtc
     2041 atctaccttc gcgcgtggat gcgcggttcc tgactacgcg atgcagcggt cgtcaggcgc
     2101 tcttgaggtt cttttggtca tggaaacgga gaactgccat gaacaagcag gtcaccttga
     2161 gcaatagcgc gtgctgcctg gctctc
//
LOCUS       DASPFR010000144         4447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1019674, whole genome shotgun sequence.
ACCESSION   DASPFR010000144 DASPFR010000000
VERSION     DASPFR010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019674"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VD737_03605"
     CDS             complement(<1..329)
                     /locus_tag="VD737_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09673.1"
                     /translation="MNDHRDETGFNELERRARAAFDASVESVDARSRSRLNQARQRAV
                     AAVSGAPARQARRGWTTWAPAGALAAGVLVVALLMRGPTGDEGLTPVASHPAALGHEP
                     REMLAAG"
     gene            complement(326..955)
                     /locus_tag="VD737_03610"
     CDS             complement(326..955)
                     /locus_tag="VD737_03610"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXW09674.1"
                     /translation="MTRFVSLEPSVRRRDDVEGITTKETRLDAAHARELDRFLAGVER
                     RAFRIAQLALRNVDDAHDAVQHAMLRLAEHYAGRPSEEWRPLFFRILYNGIRDIQRRQ
                     GVRRRFFGWLPGTTAHAEEDAPDPLEQVPDAGPEPSRLLEADEAMAELQRALQRLPAR
                     QLEAFSLRCLEGLDVAATAAAMGCSEGSVKTHYFRALQTLRSELAEVWT"
     gene            1303..3510
                     /locus_tag="VD737_03615"
     CDS             1303..3510
                     /locus_tag="VD737_03615"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004067.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HXW09675.1"
                     /translation="MSAILGVAIDAPLRRLFDYHPPPDTDAGQLRPGQRVWVPFGRRK
                     TVGVVLELRDHSALPGAQLKRALSLIDAEPVIDAALLELLRWSAEYYRHPIGEVIAAA
                     LPVALREGTDALADVERWVLSAAARTGSVTPLGPRAHKLRELAQWLQGREPAGAAELA
                     TLSTRWREHLRELERRGWVTRVRQAVHETDAAQTTGVRSPGPLLTPEQDAAVAAIDAA
                     RGGYAPFLLHGVTGSGKTEVYLRAIEGALARAEQALVLVPEISLTPQLVSRFAARLPG
                     PLAVLHSGLTDADRLRAWRAARSGVAPVVIGTRSAVFAPLFRPGLIVVDEEHDPSYKQ
                     QDGFRYSARDLALARAQRHGIPVVLGSATPSLESLERVRSGKARQLPLPTRAAGAKPP
                     ELRLVDLRAHGNTQGIATPTMLAIQRHLDRGGQVLVYLNRRGYAPTLFCPACGWVAPC
                     PRCDARLTVHQRERSLECHHCGTQRAIPESCPDCGEAVKPVGQGTERVEETLASMFPA
                     APLARIDRDAVRRRGSLEATLQRIQAGEVRLLVGTQMLTKGHHFPDVTLVVVLNADQG
                     LFGTDFRAAERLAQTIVQVAGRAGRAERPGEVLIQTEYPQHPLLRQLVQGGYDAFAAA
                     ALVEREQAGWPPFARVAVLRAEATRREMPLQFLDEARELAAGVGESGVELLGPALAPM
                     ERRAGHHRAQLLLHAATHSPLQRLLARWVPLLEKTPTARKVRWALDVDPLELF"
     gene            complement(3538..4221)
                     /locus_tag="VD737_03620"
     CDS             complement(3538..4221)
                     /locus_tag="VD737_03620"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09676.1"
                     /translation="MRKNTLAVVISSVLLSSAAIAQGGEPTSAYPTNDTSVSGTTTTT
                     PDANTATGTSTTGSMDADQGTSSSTSTTTDSSTGYESSSPSSSSNPGADTSATAQTSG
                     TSNASDNALSSTHHWTKADVNGDGELSQDEIQAVAPTLAASFTTMDSDSNGKLSRDEM
                     KAYESAHPGNSMEADQGTSSSSSSSTDSTTSSSESSSSATSGDTSSMSDDPTSTTTGE
                     DNTSGTTGQ"
BASE COUNT          617 a         1551 c         1567 g          712 t
ORIGIN      
        1 tcgccggccg ccagcatctc gcgcggctcg tgcccaagcg cggcgggatg cgacgcgacc
       61 ggcgtgaggc cctcgtcgcc cgtcggtccg cgcatcagca gcgccaccac gagcacgccc
      121 gcggccagcg cacccgcggg cgcccacgtc gtccagccgc gacgtgcctg ccttgcgggc
      181 gcgccggaga cggcggcgac cgcacgctgt cgagcctggt tcaggcgcga ccgcgaccgg
      241 gcgtcgaccg actcgacgct cgcgtcgaac gctgcgcgcg cacggcgctc cagttcattg
      301 aatccggttt cgtcgcgatg gtcgttcatg tccagacctc ggccagttcc gaacgcagcg
      361 tctgcagcgc ccggaagtaa tgcgtcttta cgctgccctc ggagcatccc atcgccgccg
      421 cggtcgcggc gacgtcgaga ccttcgagac agcgcagcga gaatgcctcc agctgccggg
      481 ccggcagccg ttgcagcgcg cgctgcaact cggccatggc ctcgtccgct tccagaagcc
      541 ggctcggctc gggaccggcg tcgggcacct gctcgagcgg gtccggcgcg tcctcttcgg
      601 catgggccgt ggtcccgggc agccagccga aaaatcgccg gcgaacgcct tgccggcgct
      661 ggatgtcccg gatgccgttg tagaggatcc ggaagaacag cgggcgccat tcctcgctcg
      721 gccgcccggc gtagtgctcg gccagccgca acatcgcgtg ctgcacggcg tcgtgggcgt
      781 cgtcgacgtt gcgcagggcc agctgcgcga tccggaacgc gcgacgttcg acacctgcca
      841 ggaagcggtc gagttcgcgg gcatgggcag cgtccagtcg ggtctccttg gtcgtgatgc
      901 cctcgacgtc gtcgcggcgg cggaccgagg gttccagact aacaaaacgc gtcatgcctt
      961 agactcgcgc tccacgccgc cacgccgctc aacgatcgtc ccagaccaga acgcctgtgc
     1021 ggacaggggg ttgacgcggg ccgcttcggc acgagatgtg caccgaccgg gacgacgggg
     1081 ggtcccaggc gtccgcggct gcgtaatgcg aactggacgc agtcttggac gccctgggtc
     1141 ggacagcaca agtccatgat tttgctgtga atgactttgg acgctcaaac gttgagcacc
     1201 gtatagaacc tgctggcgac atcggcactt ggcccacatt cccgcaaccc atccacagag
     1261 ttatccacag agtttgtgga caacggcgca ccgggccgaa tcgtgagcgc gatcctcggc
     1321 gtcgcgatcg acgcaccgct gcgccgcctt ttcgactacc acccgccgcc cgacaccgac
     1381 gccgggcagc tgcgtccggg tcagcgggtc tgggtgccct tcggccggcg caagaccgtg
     1441 ggcgtcgtgc tggagctgcg cgaccactcg gccctgcccg gggcccagct caagcgtgcg
     1501 ctctcgctga tcgacgccga gcccgtgatc gacgcggcgt tgctcgaact gctgcgctgg
     1561 tctgccgagt actaccgcca cccgatcggt gaagtgatcg cggccgcatt gccggtcgcc
     1621 ctgcgcgaag gcaccgatgc cctggccgac gtagaacgct gggtcctgag cgcagccgcc
     1681 cgcacggggt cggtcacgcc gcttggtccg cgcgcgcaca agctgcgcga gctcgcgcag
     1741 tggctgcagg ggcgcgagcc cgccggagcg gccgaactcg cgacgttgtc cacccgatgg
     1801 cgcgaacacc tgcgcgagct ggagcgccgc gggtgggtga cgcgcgtccg acaggccgtg
     1861 cacgagacgg acgctgcaca gacgaccgga gtgcggtcgc cggggccgct cctgacgccg
     1921 gaacaggacg ccgccgtggc cgcgatcgac gccgctcggg gaggttacgc gcccttcctg
     1981 ctgcacgggg taaccggcag cggcaagacg gaggtctacc tgcgcgcgat cgagggcgcg
     2041 ctcgcccgcg cggaacaggc cctcgtgctg gtgcctgaga tctcgctgac gccgcagctc
     2101 gtgtcgcggt tcgccgccag gttgccgggg ccgctggccg tgctgcactc ggggctgacc
     2161 gacgcggatc ggctgcgggc ctggcgcgcg gcacgctcgg gcgttgcgcc ggtggtgatc
     2221 ggcacgcgct cggccgtgtt cgcgccactg ttccgccccg gcctgatcgt cgtcgacgaa
     2281 gagcacgatc cttcgtacaa gcagcaggac ggcttccgct attcggcgcg cgatctcgcg
     2341 ctggcacgcg cacagcgcca cggcattccc gtcgtgctcg ggtcagccac gccgtcgctc
     2401 gagagcctcg agcgcgtgcg ctccggcaag gcgcgccagc tgccgctgcc gacgcgcgcc
     2461 gcgggcgcaa agccgccgga gctgcggctg gtcgacctgc gcgcacacgg caacacccaa
     2521 ggcatcgcga cgccgacgat gctcgccatc cagcgtcacc tggaccgcgg tgggcaggtc
     2581 ctcgtctacc tgaaccgtcg cggctacgca ccgaccttgt tctgcccggc gtgcggctgg
     2641 gtcgcgccgt gcccgcgctg cgatgcgcgg ctcacggtgc accagcgcga gcggtcgctc
     2701 gagtgccacc attgcggcac gcagcgcgcg attcccgagt cgtgcccgga ttgcggagag
     2761 gccgtcaagc ccgtgggcca gggcaccgaa cgtgtcgagg agacgctggc gagcatgttc
     2821 ccggcggcgc cgctcgcgcg gatcgaccgc gatgcggtcc gtcgccgagg gtcactggaa
     2881 gccacgttgc agcgcatcca ggccggcgaa gtgcggctgc tcgtcggcac gcagatgctg
     2941 accaagggcc accacttccc ggacgtcacg ctggtcgtgg tgctgaacgc ggaccagggg
     3001 ttattcggca ccgatttccg cgccgcggaa cgtctcgcgc agaccatcgt gcaggttgcc
     3061 ggtcgcgccg gtcgggcgga gcggcccggc gaggtcctga tccagaccga gtacccgcag
     3121 cacccgttgc tgcggcagct cgtccagggg ggctacgacg cgttcgccgc ggcggcgctg
     3181 gtcgaacgtg agcaggccgg ctggccgccc ttcgcccgcg tggcggtgct gcgcgcggag
     3241 gcgacgcgtc gcgagatgcc gctccagttc ctggacgagg cccgggaact cgccgccggt
     3301 gtcggtgaat ccggtgtcga gctgcttggg cctgcgctcg cgccgatgga gcgccgcgcg
     3361 ggccaccatc gcgcgcagct gctgctgcac gccgcgacac actcgccatt gcagcgcctg
     3421 ctcgcgcgct gggtgccgct gctcgaaaaa acgcccaccg cacgcaaggt gcggtgggcg
     3481 ctggacgtgg atccgctcga gctgttctag ggcacgagct tcgtcacgag agcgaggtta
     3541 ctggccggtc gtgccggacg tgttgtcctc gccagtggtg gtggacgtcg ggtcgtcgct
     3601 catcgacgag gtgtcgcccg aggtcgcgct ggagctcgac tccgagctgg aggtcgtcga
     3661 gtcggtggac gaagaggaag aagagctcgt gccctggtca gcctccatgc tgttgcccgg
     3721 gtgcgcgctc tcgtacgcct tcatctcgtc gcgcgagagc ttgccgttgc tgtccgaatc
     3781 catcgtggtg aagctcgcgg ccagcgtcgg cgccaccgcc tggatctcgt cctgcgacag
     3841 ctcaccgtca ccgttcacgt cggccttcgt ccagtggtgc gtcgagctca gcgcgttgtc
     3901 gctggcattc gacgtgccgg acgtctgtgc cgtcgccgag gtgtcggcac ccggattgga
     3961 cgaagagctc gggctcgagc tttcgtagcc cgtgctgctg tccgtcgtcg tggacgtgga
     4021 agagctggtg ccctggtccg catccatgct gcccgtcgtg ctcgtgcccg tcgccgtatt
     4081 ggcgtccggc gtcgtcgtcg tcgtgcccga caccgacgtg tcgttggtcg gataggcgct
     4141 ggtcggctca ccgccctggg cgatcgcggc cgagctcagc aggacggacg agatgacaac
     4201 tgcgagtgtg ttcttgcgca ttgaggtaac cctccttggt cgtggaaaca gtgtgaccgg
     4261 ggttcgcgga ccgtgttccg gggtcgaggt gtctgccttt ggcgacatcg cggaaacacg
     4321 aggaggcgcg ccaactatcg gatgtaattg aaattttttc cggcgtccct ttcgaacaga
     4381 cgcggccccg agggccttcg ctcgcgtccg tgtcgaccta caggccgtcc gcggaggccg
     4441 gccgatc
//
LOCUS       DASPFR010000145         2206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1751744, whole genome shotgun sequence.
ACCESSION   DASPFR010000145 DASPFR010000000
VERSION     DASPFR010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751744"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VD737_03625"
     CDS             complement(<1..472)
                     /locus_tag="VD737_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HXW09677.1"
                     /translation="MLATLLSRTPQGLEAPLVRVEVDVGSGLPAFSVVGLLETAVKES
                     KDRVRAALANCGYEFPAGRVTVNLAPADLPKEGGRFDLAIAIGILAAADLLPAAPFDG
                     VELYGELSLGGDVHATRGILPSALQAGRSGHAMIVPRVNAREAALARSARVLMAG"
     gene            544..1518
                     /gene="cysB"
                     /locus_tag="VD737_03630"
     CDS             544..1518
                     /gene="cysB"
                     /locus_tag="VD737_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012313669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HXW09678.1"
                     /translation="MKLHQLRYLAAVAQTGLNITAAAEKLHTSQPGVSKQIKLLEDEL
                     GFQIFVRDGRNLSRVTPAGQQVIDRAIRILREVQNIKRLSDDLKDEERGTLSIATTHT
                     QARYVLPEVVKAFRERFPEVRLHLHQGTSEQIAEMASLDRIDFAIATGSEGLFDGFAL
                     LPCYKWHRHVVVPRNHPLARAKKITLKQLASHPIITYVFSFTGPSSLHGIFAKEGLVP
                     NVVLTARDADVIKTYVRLGLGVGIVASMAIEAEGDADLVHIDTSDLFPTHTTWIGFRR
                     GGLLRKYQLDFIQLFAPHLTRRVIERAAGAANPAEVEQLVAGLELPLK"
     gene            1705..>2206
                     /locus_tag="VD737_03635"
     CDS             1705..>2206
                     /locus_tag="VD737_03635"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HXW09679.1"
                     /translation="MILSPASRWTAAAIAAAALAPVTASRAEPSTDELLQKIDDLSQK
                     VLVLERKLEIQNEATQTSVKSSPVVRASPKGYSIESPDKQNVVKIRGTLHFDSRHFED
                     DVTAETADTFLLRRVRPTIEGTLGGIYDFRFTPDFAGGRTIILDAFASARFKPWFNVT
                     GGKFKVP"
BASE COUNT          396 a          767 c          701 g          342 t
ORIGIN      
        1 gtcctgccat caatacgcgc gcactgcgcg cgagcgcagc ctcacgtgcg ttgacgcgag
       61 gcacgatcat cgcgtgacct gagcgtccgg cctgcagcgc gctcggcagg atgccgcgcg
      121 tggcgtgcac gtcgccgccg agggagagtt cgccgtagag ttccacgccg tcgaacgggg
      181 ccgccggcag caggtccgcc gccgcgagga tgccgatcgc gatcgcgagg tcgaaccgtc
      241 cgccttcctt cggcaggtcc gcgggcgcca ggttgaccgt gacgcgaccc gcggggaatt
      301 cgtagccaca gttcgcgagt gccgcgcgca cgcgatcctt gctttccttg acggccgttt
      361 cgagcaggcc gacgaccgag aacgcgggca ggccgctgcc gacgtcgact tcgacacgga
      421 cgagcggcgc ctcgaggccc tgcggggtgc gactcagcaa cgtggccagc atcaccgacc
      481 atatccgcca cggcccgctg gcgctcaagc tgcgaaacgg cgtggttcgc ttagaatgac
      541 tgcatgaaac tgcaccagct gcgctacctg gccgcggtcg cccagaccgg cctcaatatc
      601 acggccgcag ccgagaagct gcacacgtcg cagccgggcg tcagcaagca gatcaagctg
      661 ctcgaggacg aactgggatt ccagatcttc gtgcgcgacg gacgcaacct gtcgcgggtc
      721 acgcctgccg gccagcaggt gatcgaccgg gcgatccgga tcctgcgcga ggtgcagaac
      781 atcaagcggc tgtccgacga cctcaaggac gaggagcgtg gcacgctctc gatcgccacc
      841 acgcatacgc aggcgcgtta cgtgctgccg gaagtcgtga aggcgtttcg cgagcgcttt
      901 ccggaggtcc gtctgcacct gcaccaggga acgtccgagc agatcgccga gatggcgtca
      961 ctcgaccgga tcgacttcgc gatcgcgacc ggctccgagg gtctgttcga cggctttgcg
     1021 ctgctgccct gctacaagtg gcaccgtcac gtcgtggtgc cccgcaacca tccgctcgcg
     1081 cgcgcgaaga agatcacgct caagcaactg gcctcgcatc cgatcatcac gtacgtcttc
     1141 agcttcacgg gcccgtcgtc gctgcacggg atcttcgcca aggaaggact ggtgcccaac
     1201 gtcgtgctga cggcccgcga tgccgacgtc atcaagacgt acgtgcgcct cggcctcggc
     1261 gtcggcatcg tcgccagcat ggcgatcgag gcggaaggcg acgccgacct cgtgcacatc
     1321 gacacctccg acctgttccc gacgcacacg acgtggatcg gcttccggcg cggcggcctg
     1381 ctgcgcaagt accagctcga cttcatccag ctgtttgcgc cgcatctgac tcgacgcgtg
     1441 atcgaacgcg cagcaggcgc cgcgaatccg gccgaggtcg aacagctcgt cgccggcctg
     1501 gaactccctc tgaagtaacg cgagccggct gcgggcgcgc cgcttccaca aatgcccgtc
     1561 tcgctgtccg ccagaaacgc aaatccaagt tgcggcacgt gccgaggcga cgcgtaaccg
     1621 gttcctaacc gccggtcgac tgacgctgtc acccgccctc cctaggatcg ctctcctgag
     1681 tcgccacaac acggagaacg accgatgatc ctttcgcccg ccagccggtg gaccgctgcc
     1741 gcaatcgccg cggcagcgct tgcacccgtg accgcgagcc gcgccgagcc gagcaccgac
     1801 gaactgctgc agaagatcga tgacttgagc cagaaggtgc tggtgctgga acgtaagctc
     1861 gagatccaga acgaggcgac gcagacgtcg gtcaagtcat cgccggtcgt cagggccagc
     1921 ccgaagggtt acagcatcga gtcgccggac aagcagaacg tcgtgaagat ccgcggcacg
     1981 ctgcacttcg acagccggca cttcgaggac gatgtcacgg ccgagaccgc cgacactttc
     2041 cttttgaggc gcgtccgccc gacgatcgag ggcacgctcg gcggtatcta cgacttccgc
     2101 ttcacgccgg acttcgcggg cggccgcacg atcatcctcg acgcgttcgc ctcggccagg
     2161 ttcaagccct ggttcaatgt gaccggcggc aagttcaagg tgccag
//
LOCUS       DASPFR010000146         5889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_652886, whole genome shotgun sequence.
ACCESSION   DASPFR010000146 DASPFR010000000
VERSION     DASPFR010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652886"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..769)
                     /gene="lolA"
                     /locus_tag="VD737_03640"
     CDS             complement(137..769)
                     /gene="lolA"
                     /locus_tag="VD737_03640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HXW09680.1"
                     /translation="MRAFLLVAAAALLVPAESRAADAVTRVDAYLDSLASLSAQFVQV
                     VQDRKGEITDRATGTLSIARPNRFRWDYREPYTQTIVADGRKLWLYDPDLEQVTVRSL
                     EQGLGATPAMLLSGSGDVADAFAAGPVEQQQGLTWCRLLPKQPGSDFERVSLAFGPRN
                     ELAAMELVDKLGQTTTIQFSAVKRATQLDESLFRFVPPAGADVIGDVAVR"
     gene            complement(784..3111)
                     /locus_tag="VD737_03645"
     CDS             complement(784..3111)
                     /locus_tag="VD737_03645"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HXW09681.1"
                     /translation="MAKADAKRADGGAQAANVARGLRESALWLFGALALILFAALVSY
                     DRADPAFSSTGQPGPVTNLIGPFGAWLSDLFFVLFGGPAFLFPIMLGFAGYRLFQERG
                     QREPVDRRSASFRSLGFFLALASSCGLATLHFSGAGFPNTAGGVVGSLLGEGLARAMS
                     FLGATLLLLAVWLGSVSLATGMSWLDAMDRIGAAVLRGLSLVRERLSERRDQNIGKEV
                     KQQREVVVREEQKKVAERKPPKIEAPAPKVEKSERVEKEKQVPMFERSGASALPALSL
                     LDDPPPRQGGYSAEALEAMSRLVELKLRDFGVEAEVVEVHPGPVITRFELRPAPGDKV
                     AQISNLAKDLARSLSAISVRVVEIIPGKSTMGLEIPNEVREMVTLGEIIKSKAYDDMA
                     SPLALVLGKDIGGNPVVADLARMPHLLVGGTTGSGKSTAVNAMVLSLLYKSTPDHVRL
                     IMIDPKMLELSVYEGIPHLLAPVVTDMKQAANALRWCVAEMERRYQLMAFAGVRNLAG
                     YNRKVREARESGRPLKDPIEMKRLMPGLDPATVPDLEPLPNIVVVVDELADMMMIVGK
                     KVEELIARLAQKARASGIHLILATQRPSVDVITGLIKANIPTRIAFQVSAKVDSRTIL
                     DQSGAESLLGHGDMLFLQPGTSHPVRVHGAFVSDQEVHKVAATLREHSSPVYIDEVLD
                     GPSAPIPGLPGEAGEGGEGAEGGDAEQDPLYDEAVRIVTETRKASISGVQRRLKIGYN
                     RAARMIEGMEAAGLVGPLNSNGSREVYAPPPPEAD"
     gene            3357..4325
                     /gene="trxB"
                     /locus_tag="VD737_03650"
     CDS             3357..4325
                     /gene="trxB"
                     /locus_tag="VD737_03650"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020796668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HXW09682.1"
                     /translation="MTAPRHSRLIILGSGPAGYSAAVYAARANRNPLLITGVEQGGQL
                     MTTTEVDNWPGDPHGLTGPVLMDRMRQHAERFHTEILMDHINEVDLGRRPLLLKGDRG
                     DYTCDALIIATGASAQYLGLASEEQFRGRGVSACATCDGFFFRGQRVAVVGGGNTAVE
                     EALYLSNIASHVTLIHRRDKLRAEKILQDHLFERQRAGVVDIVWDHTVDEVLGDQTGV
                     TGVRVTHKDGTGTRDLTVAGLFVAVGHKPNTDMFAGQLDMNGGYILVRAGLTGGATAT
                     SVPGVFAAGDVADHVYRQAVTSAGTGCMAALDADKYLDSLDAGAAH"
     gene            4353..5531
                     /locus_tag="VD737_03655"
     CDS             4353..5531
                     /locus_tag="VD737_03655"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXW09683.1"
                     /translation="MPLKAEVVGRIADVDAAEWDALDPSGHPFTRHAFLEALESTGCV
                     GEDTGWIPAHLVVRDSRKSHARVVAAVPQYLKTHSWGEFVFDWSWAQSYLRAGLDYYP
                     KQLSAIPFTPVTGPRLLIDAVATGLDETDLRDELAGLLLETARRTGSSGAHVNFTLEA
                     DQAALERAGFLRRHDCRFLWRNREYRSFDDFLATFRADKRKKAKRERRKVADAGITFR
                     TLRGEAIDAALWRVIFGFSERTFLRHGNGHYLNLEFLERVSRALPGSVMVKVAERAGR
                     PVAASIFFEGGGWLYGRYWGASAHEDSLHFEACYYQGIDYCIERGLQWFDPGTQGEHK
                     IARGFEPTRTLSAHWLEHAGFRTAIARYLERERDAIDAYIADSTGHVPYQRAVAGADA
                     "
     gene            5528..>5889
                     /locus_tag="VD737_03660"
     CDS             5528..>5889
                     /locus_tag="VD737_03660"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HXW09684.1"
                     /translation="MIPWLRMSDPPEAFPAVSEALEDPDGLLCAGGDLSPERLLAAYR
                     RGIFPWYAEGQPILWWSPDPRMVLFPDELRVTRSLRKSLRNRGYRVTFDAAFADVLSQ
                     CADNGLRAFEGTWISPDM"
BASE COUNT          925 a         2079 c         2002 g          883 t
ORIGIN      
        1 cgtgcggccc gccggtcgcg aacatcgtct gcaggctgcc ggtgacgacg gtcacgacga
       61 tcaccgcgct cgttgcgatc cagaaccagc ggtgccgcag ggccagcatg cgtgcgcgca
      121 acggaatcgc ggtgcgtcag cgcaccgcga cgtcgccgat cacgtcggcg ccggcgggag
      181 gcacgaagcg gaacagcgac tcgtcgagct gcgtcgcgcg cttcaccgcc gagaactgga
      241 tcgtcgtggt ctggccgagc ttgtcgacga gttccatggc ggcgagttcg ttgcgtgggc
      301 cgaaggcgag gctgacgcgc tcgaagtcgg agccgggctg cttcggcagc agccggcacc
      361 aggtgaggcc ctgctgctgc tcgacgggcc cggccgcgaa ggcgtcggcg acgtcacccg
      421 aaccggagag cagcatggca ggcgtcgcgc ccaggccctg ctcgagcgac cgcaccgtca
      481 cctgctcgag gtccgggtcg tacagccaga gcttgcggcc gtcggcgacg atcgtctgcg
      541 tgtacggctc gcgatagtcc cagcgaaagc ggttgggccg cgcgatcgac aacgtgccgg
      601 tggcgcgatc ggtgatctcg cccttgcggt cctgcacgac ctgcacgaac tgcgccgaca
      661 gcgacgccag ggaatcgagg tacgcgtcga cgcgcgtgac cgcgtcggct gcgcgggact
      721 cggcgggcac gagcagggcc gcggccgcca ccagcaggaa tgctctcacg gtatcgacag
      781 gcctcagtcc gcctcggggg gaggtggtgc gtagacctcc cgcgaaccgt tggagttcaa
      841 gggaccgacc agccccgcgg cctccatccc ctcgatcatg cgcgcggcgc ggttgtaacc
      901 gatcttgagc cggcgctgga cgccggaaat cgacgccttg cgggtctcgg tgacgatccg
      961 gacggcctcg tcgtacaacg ggtcctgctc ggcgtcgccg ccctccgcgc cttcgccccc
     1021 ctcgcccgcc tcgccaggca gcccgggaat cggcgcagac gggccgtcga gcacctcgtc
     1081 gatgtagacg ggcgagctgt gctcgcgcag ggtcgcggcc accttgtgca cttcctggtc
     1141 ggagacgaac gcgccgtgca cgcgcacggg atgcgacgtg cccggctgca ggaacagcat
     1201 gtcgccgtgg ccgagcaggc tctcggcacc gctctggtcg aggatcgtgc gcgagtcgac
     1261 cttggccgac acctggaacg cgatccgcgt cgggatgttg gccttgatca ggccggtgat
     1321 cacgtcgacg gacggccgct gcgtggcgag gatcaggtgg atgcccgacg cgcgcgcctt
     1381 ctgcgccagc cgcgcgatca gctcctcgac cttcttgccg acgatcatca tcatgtcggc
     1441 gagctcgtcg acgaccacga cgatgttcgg cagcggctcg aggtccggca ccgtcgccgg
     1501 gtcgagtccc ggcatcagcc gcttcatctc gatcgggtcc ttgagcggcc ggcccgactc
     1561 ccgcgcttcg cggaccttgc ggttgtatcc agccaggttg cgcacgcccg cgaacgccat
     1621 caactggtag cggcgctcca tctcggccac gcaccagcgc agcgcgttgg ccgcctgctt
     1681 catgtccgtg acgaccggcg cgagcaggtg cgggatgccc tcgtacaccg agagttccag
     1741 catcttcgga tcgatcatga tcagtcgcac gtggtccggc gtcgacttgt agagcagcga
     1801 cagcaccatc gcgttgaccg cggtcgactt gcccgacccc gtggtgccgc ccacgagcag
     1861 gtgcggcatg cgcgcgaggt cggcgacgac cgggttgccg ccgatgtcct tgccgagcac
     1921 gagcgccagc ggcgaggcca tgtcgtcgta ggccttcgac ttgatgatct cgccgagcgt
     1981 gaccatctcg cggacctcgt tcgggatctc gaggcccatc gtcgacttgc cggggatgat
     2041 ctcgacgacg cgcacgctga ttgccgagag cgagcgcgcg aggtccttgg cgaggttcga
     2101 aatctgcgcg accttgtcgc cgggcgccgg tcgcagttcg aagcgcgtga tgaccgggcc
     2161 ggggtgcacc tcgaccacct cggcttcgac gccgaaatcg cggagcttca gttcgaccag
     2221 gcgcgacatc gcctcgagcg cctcggccga gtagccgccc tgccgcggcg ggggatcgtc
     2281 gagcagtgag agcgccggca gcgccgacgc gcccgaccgc tcgaacatcg gcacctgctt
     2341 ttccttctcg acccgctcgc tcttctcgac cttgggcgcg ggagcctcga tcttcggcgg
     2401 cttgcgctcg gccaccttct tctgctcctc gcgcacgacc acttcgcgct gctgcttcac
     2461 ttccttgccg atgttctggt cgcgccgctc ggacaggcgt tcgcgcacca gcgacaaccc
     2521 gcgcagcacc gcggcgccga tccggtccat ggcgtcgagc caggacatcc cggtggcgag
     2581 cgagaccgag cccagccaga cggcgagcag cagcagcgtc gcgcccagga agctcatcgc
     2641 ccgggcgagg ccttcgccga gcagcgagcc cacgacaccg ccggccgtgt tcgggaaccc
     2701 ggcgcccgag aaatgcagcg tggcgaggcc acagctgctc gcgagcgcca aaaagaagcc
     2761 caggctgcgg aacgaagccg accgtcggtc gaccggctcg cgctggccgc gctcctggaa
     2821 cagccggtat ccggcgaacc cgagcatgat cgggaacagg aaggccggcc cgccgaacag
     2881 cacgaagaac aggtcggaca accaggcgcc gaacgggccg atcaggttcg tcaccggacc
     2941 gggctggccg gtcgaggaga acgcggggtc ggcgcggtcg tagctcacga gcgcggcgaa
     3001 caggatcagg gccagcgcgc cgaacaacca cagtgcgctc tcgcgcaacc cccgggcgac
     3061 gttcgccgcc tgcgcaccgc cgtcggcgcg cttcgcatcc gccttagcca cctgtcatca
     3121 cctgaaggtt caagagttgc tcgtaacccc ttgtgtcgca aggctacacc aaaaaggggg
     3181 acactccgaa attttcaaac ggaaatggag acaaaggtcc gctggagccg cagcggcgtc
     3241 gctgaccgaa aggcatatgc agagccgcag ccgaactttc cgagcggcct gcgagtgctt
     3301 accatgccgc gccttcgtcg acgcgttgcg cgccgcaaaa cagcaaggaa ttgtacatga
     3361 ccgctccccg ccactcccgg ctgatcatcc tgggctcggg tccggccgga tactccgccg
     3421 ccgtctacgc cgcgcgcgcc aaccgcaacc cgctgctgat caccggcgtc gagcagggcg
     3481 ggcagctgat gacgacgaca gaggtcgaca actggccggg cgacccgcac gggctgacgg
     3541 gaccggtgct catggaccgc atgcgccagc acgccgagcg gttccacacc gagatcctga
     3601 tggatcacat caacgaggtg gacctgggcc gccgcccact gttgctcaag ggtgaccgcg
     3661 gcgactacac ctgcgacgcc ctgatcatcg cgaccggagc gagcgcgcag tacctcgggc
     3721 tggcgtccga ggagcagttc cgcggccgcg gcgtgtcggc ctgcgccacc tgcgacggct
     3781 ttttcttccg tggccagcgc gtggcggtcg tcggcggcgg caacacggcc gtcgaggagg
     3841 cgctgtacct ctcgaacatc gcctcgcacg tcacgctgat ccaccgccgc gacaagctgc
     3901 gcgccgagaa gatcctccag gaccacctgt tcgaacgaca gcgcgccggc gtggtcgata
     3961 tcgtctggga tcacacggtg gacgaagtgc tgggtgacca gacgggcgtg acgggggtgc
     4021 gggtgacgca caaggacggc acggggactc gcgacctcac cgtcgcgggc ctgttcgtgg
     4081 ccgtgggtca caagcccaac acggacatgt tcgcgggtca gctcgacatg aacggcggct
     4141 acatcctcgt gcgcgcgggc ctcacgggcg gcgcgacggc cacgagcgtg ccgggcgtgt
     4201 tcgcggccgg cgacgttgcc gaccacgtgt accggcaggc ggtcacctcg gccggcaccg
     4261 gctgcatggc cgcgctcgat gccgacaagt acctggactc actggatgcc ggcgccgccc
     4321 actgaggcag ttcgcgcagg cggggcagcc cgatgcccct gaaggcggaa gtcgtcgggc
     4381 gcatcgcgga cgtcgacgcg gccgagtggg acgcgctcga tccgtccggt cacccgttca
     4441 cgcgacacgc gttcctcgag gcgctcgagt ccaccggctg cgtcggcgag gacaccggct
     4501 ggatcccggc gcacctggtc gtgcgggact cgcggaagag ccacgcgcgc gtggttgccg
     4561 ccgtgccgca gtacctgaag acgcactcgt ggggcgaatt cgtgttcgac tggagctggg
     4621 cgcagtcgta cctgcgcgcc ggcctcgact actatccgaa gcagctttcc gccatcccct
     4681 tcacgcccgt caccggcccg cgcctgctga tcgacgccgt tgccacgggc ctcgacgaaa
     4741 cggaccttcg tgacgagctc gcagggctgt tgctcgaaac cgcgcgccgc accgggtcgt
     4801 ccggggcgca cgtgaacttc acgctcgagg ccgaccaggc cgcgctcgag cgcgcaggct
     4861 tcctgcgccg gcacgactgc cgcttcctct ggcgcaaccg cgagtaccgc agcttcgacg
     4921 atttcctggc cacgttccgc gccgacaagc gcaagaaggc gaagcgcgag cggcgcaagg
     4981 tcgccgatgc gggcatcacc ttccgcacac tccggggcga agcgatcgac gcggcactct
     5041 ggcgcgtgat cttcgggttc tcggagcgca cgttcctgcg ccacggcaac ggtcattacc
     5101 tcaacctgga gttcctcgag cgcgtctcgc gcgccctgcc cggcagcgtc atggtcaagg
     5161 tggccgaacg cgcaggacgg cccgtggcgg catcgatctt cttcgagggc ggtggctggc
     5221 tgtacggccg ctactggggc gcgtcggccc acgaggactc gctgcacttc gaggcctgct
     5281 actaccaggg catcgactac tgcatcgagc gtggactgca gtggttcgac ccgggcacgc
     5341 agggcgagca caagatcgcg cgcggcttcg agcccacccg cacgctctcg gcacactggc
     5401 tcgagcacgc gggctttcgc acggcgatcg cgcggtacct ggaacgcgag cgcgatgcga
     5461 tcgacgcgta catcgccgac tcgaccgggc acgtccccta ccagcgcgcg gtcgcgggag
     5521 ccgacgcgtg atcccttggc tgcgcatgtc ggatccgccc gaggcgtttc ctgcggtcag
     5581 cgaagcgctc gaggatcccg acggcctgct ctgcgccggc ggcgacctga gtcccgagcg
     5641 gctgctcgcc gcttaccggc gcgggatctt tccctggtac gccgagggcc agcccatcct
     5701 ctggtggtcg ccggacccac gcatggtgct gtttcccgac gaactgcgcg tcacgcgcag
     5761 cctgcgcaag agcctgcgca accgcgggta ccgcgtgact ttcgatgcag cgttcgccga
     5821 cgtcctctcg cagtgcgccg acaacggcct gcgggcgttc gagggcacct ggatttcacc
     5881 cgacatgca
//
LOCUS       DASPFR010000147        17244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1509122, whole genome shotgun sequence.
ACCESSION   DASPFR010000147 DASPFR010000000
VERSION     DASPFR010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17244
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509122"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1979)
                     /locus_tag="VD737_03665"
     CDS             complement(12..1979)
                     /locus_tag="VD737_03665"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09685.1"
                     /translation="MSTTVFDARGYPERNDAPAGKFERLLTELRYLAGRAESGTRQLS
                     RIIQKVEGYREQTTSASDAELRARAAALRPQLRRRGFDDLALAAEAFALVREASVRTT
                     GLRHFDVQLIGGWAMLRGMVAEMDTGEGKTLTATLTAATAALAGRAVHVVTVNDYLAE
                     RDATQMGPVYRLLGLSVGCVKQGQQPAERRAAYACDVAYCSNKEVAFDYLRDRLVLGG
                     KPHPIALRLGALAGGPGDGNAGGGRLLLRGLQFAIVDEADSVLVDEARTPLILSGGSD
                     RTGSEQLHREALEIAGTLGEPDFSLGEEGVEITEAGERKLARAGKDLGGVWKGPRRRE
                     QLVRQALTALHVFQRDKHYLVRDGKVQIVDEYTGRLMPDRSWERGLHQLIEIKEGVEV
                     TPVRETLARISYQRFFRRYLHLCGMTGTAAEIAGELRAVYRLRVARVPTNRPTRRSRR
                     SSRYFGSAAAKWQTVVEAIAERSARGQPVLVGTRSVKASEHLAKLLQERNISFRLLNA
                     RQDRDEAEIVARAGERGCVTVATNMAGRGTDIRLGEGVPELGGLHVIATELHDAARID
                     RQLFGRCGRQGDPGTYEAIVALEDDLVKSFAPLVHDGFARGAGVPAALGAAVFRLAQW
                     RAERAHSRARRDLLDLDDYLGDILAFSGRGE"
     gene            complement(1976..4114)
                     /locus_tag="VD737_03670"
     CDS             complement(1976..4114)
                     /locus_tag="VD737_03670"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HXW09686.1"
                     /translation="MSDLHSPSWYRVAQLRPRLRGHARIHRHHYRGELWYVLEDRVSR
                     RMHRFNPAAYYIVGLLDGRRTVHEIWEAATERFGDDAPTQDEVIRLLGQLHAAEVLQT
                     EAMPDLTELARRARKGKRKTWLQNLLSPLAIRFPLVDPDRFLERWLPWYGPWFGWVGL
                     AVWAVVVGFGIATAAGHWDELTADVTSQILAPQNLLILWLTFPVVKLLHELGHACAVK
                     AWGGEVHEMGIMLLVLMPIPYVDASAASAFPRARRRVVVGAAGMLVEVFLASIAIQLW
                     AEAEPGLARAVLYDVMLIAGISTIVFNANPLLRYDGYYMLADLVQIPNLRVRANQYLG
                     RLVERRLFGVNLDEEPTPAAERAWLGFYAVLSFLYRTFVICAIALFIASEYLLVGVLL
                     ALWALATAVVLPVLKGVGYLLMHPRLRRRRPRALATTALALALLYGALFVLPVPLWTR
                     AEGVIALPEESHLRAGAEGFVRKIVAAPGSIVGAGTELVITEDPMLALRERVLEAEVR
                     LLEARANALRLENRVRWAMTLDELEAARRQLEEVRTRLAELTISSPVAGTFVLAHAAA
                     DLPERFLRRGEQVGYVLPAKAATARVLVSQDDVDLVRSRTLRIEAKVAGRLYDTYEAR
                     LVREVPAASNRVSNLALSSAGGGGAALDPSGKEPKTLDPWFAFELELPQTPTLTLGEH
                     VYVRFEHGGEPLAGRLYRSVRQLFMRQFTV"
     gene            complement(4115..5995)
                     /locus_tag="VD737_03675"
     CDS             complement(4115..5995)
                     /locus_tag="VD737_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXW09687.1"
                     /translation="MSPVQPEIQENRRLASVAELASRRGADQAWARFAQAQSHEEFCS
                     SWLAIQCDAIGGVSDGVVVLQTPGARDFVPVAFHPDAPRDRAHLAEIAERTLKEARGV
                     VLPLGPSQSEPRYQLGYPVRLDGEVRGAVGLEMDWRPEPQLHLAMRSLQWGSGWLEVL
                     LRRHADPAAAAQLRLKLALDLVATLLEQGALRESAAAFATEVATRLGCERAAVGLLKG
                     RAIKVIGVSHSAQFDERANLLRAVENAMAEAVDQGELVVSPPPRDDLPVVAHAHDVLL
                     RESGAGAAATFPLVCDGEVVGALSLERAAPYRFDPESLQLGEALAAVAGPIVELKRRA
                     EANLALHAGRSTADLWKKLAGPRHPGFKLAVVLTAAAAAFLALATGTFRVSADVTVEG
                     RMQRAITAPFNGYVKEAPLRAGDRVKAGQPIGRFEDRELQLERIKLVAQREQVERQHR
                     EAMAKRDRAQTAILAAQLDQNEAQLALLEEQLARSVMTAPVDGLIVSGDLSQSLGSPV
                     ERGQVLFEVAPLDGYRIVLRVDERDMAHVALGQRGELAVTAMPGEALPFTVTSIVPVN
                     VAQEGRNVFRVEARLDGEPGRLRPGMTGVGKIHVEERRLVWIWTRSFTDWLQLKLWSW
                     LP"
     gene            complement(6033..6368)
                     /locus_tag="VD737_03680"
     CDS             complement(6033..6368)
                     /locus_tag="VD737_03680"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HXW09688.1"
                     /translation="MATKGADTAAGTDARAALEGTRIKWDDSGMKSSYANVCNVTSTR
                     EEVVLLFGMNQAWNRGQKEVTIHLTDRIVVSPFAAKRLSMLLQGVVNEYEKRFGTLNI
                     DLPGGDAKK"
     gene            complement(6455..17227)
                     /locus_tag="VD737_03685"
     CDS             complement(6455..17227)
                     /locus_tag="VD737_03685"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HXW09689.1"
                     /translation="MLTDNPFVDFGGGAILTPRIAGLAGGAEAFGLLTLDAATLLGDD
                     VFDEAPDGAGLAVIRVDTGIAGLVDNYLGYDLVLVANISGAAFDTPTLRIGSVGERAA
                     FRTGGTAADPVFGDGSDDPMTSFAAGAIYALLIPEAAQQFSILATREVDGVDVDFSAS
                     VEELNNGEVLYVREPGLTVMLNQRIAPDDVQFAGRSWESLGIVTVTPDTTTPESPAIE
                     VVITDASGGIVAADGVILRRVDTSLPGLRLITLSDNPLDQRSHDIFIEVLESRASTLG
                     LTAVNPGPVDGDFVNDLTLKFTVGLNDGTLLSGTVELSAIQSQANSDLAGLAAQLNTL
                     LADALDDLGLEDDAIVLQVVGERLSFDVTDTDIASLRVEGAEQLGFAAGQGVGPDVEF
                     TPNEAPEIAQIANQPGTPNAFTFNGTSSRITVANSASLQADGELTIEAWINPTGPGQD
                     GIIVNKEGEYEVARFSDGTIRWAFANSNPGWVWINTGYVAKLNEWTHIAVVYDNGDVR
                     TYADGELVHFFDGAGEIGDVHTGQNTLQIGDRESFTQHFQGQIDEVRVWTAVRSQTEI
                     RDAMNRILDGDEAGLAGYWTFDDQTTSTAFGSVVDYSGNGNHGTPSGGAVRSHGPIRI
                     EVRDQLGDPIYLHVVSDTPQVQAEMLDESNLLITEAPGFEGTVVITLTAFDGLGAAGD
                     PHGRHHTVTFEYTSGGNSAQAIYGTKFNDIDGDGVRDPNEPGIDGVKLFLDLNANGLL
                     DEREPVTYTDVNGEYGFRGLEVVDPVPHGPAELVAAAPAEFHGGATQTIATTTAQTEI
                     VDRTEATFQFTAQSLPDALSFDGGDRVTVAHNASTALAGSSTVEVRFRYDFDATNQFR
                     ALVHKTSASPLFRTYSMFINQNTGTLFFDTWDAQSGQQSWFLSNVVLQPGQWYEFATV
                     IDRDSTDPDDPSRGPVKAYLNGTEVLSSTVLKTPAAVTTNPLLIGDTLETNFAGFDGI
                     IDNVAIWNKPRTQDEIKADFADGIAAPAVGLAGYWQFDESGGVAIDSSGNANHGTLGT
                     GVEAPLRVDAGTLEPVPPGFSTTVILTPEQTSDNLTGADLRDDFNLAFALQKVSHLLS
                     AHFEDGVLFFQLAGGFDAFQVAASTHQTVIETLERAGAPIINTRLDEVTGGAFGFAAF
                     SEDQTPGSLGELGVAQTDITADVGLKQIVAAGEATIETETTRTTEFLLSVDGLTGYFQ
                     LMHDKTLDNDDIFDLAADLEALVNGDPVVGPLVTVGVADGRLKFTTTGVGDTRTVQLH
                     FSDTFGQSVTTTAFGNGAEHVEMFQLPIGPDPLGFGGSGFAQGTNRSYLVAEVPFPGW
                     TPTTGATSTPAGVIGVLPVLFTDAGQIVEGVDFGNQLIADFDLGEDFEVDEGDEVSFM
                     PTVVGSPGAVFTYLWEVQADNGQIIADGDEATFSFVPFDNGTYTVQLTVTDTFRGLTS
                     HPDTVIVTSLNVAPEVEAGTAQTVFEGEIVSIEPAFDDAGTNDTHTAVIAWGDGTTTI
                     FESLTETNGAGTIPATHVYADDGVYTVMVSVTDDDGATTSDTLVVTVDNVAPVVDAGP
                     DRTADEGALVSLSPLIVIGDNVVIGEDVPLPPTVRFTDSGTLDTHSATIDWGDGTAAE
                     TGLVSEVPFGPPGSEFGLTGLVLGSHVYADDGVYTVIVTVTDDNGGEHSDSFDITITN
                     VAPVIRPTAHAGREGELISLKDVTFLDPGTLDTHTGTVNWGDGTATEDLVIASAPFGP
                     PGSLGGLTGTLNGSHVYADDGEYIVTFVLHDDDGGIATETLTMIVDVVNDPPTVEAGT
                     GTTIFEGDEFSLSGASFNDKGTLDTHTASVNWGDGTTSAGTVTETPFGPPGSTAGMSG
                     TAAASHFYGDDSGAGQFTVTLTVTDDDDESGADTVLVKVLNVAPEVTAGADVEAFEGD
                     TVMVSATFTDPGFLDTHTAEIDWGDGMVTMGAVDAATKTVTGAHVYADNGPYLVTLTV
                     TDDDGGEDSDGLFVTINNAPPVVTDGAAIVDEGVPLALLIPFTDPGSGDTHTALVDFG
                     DGTLPVLFPNVTGSVSVSHTYADNGDFTVTIAVTDDEGATGTGTWEVTVNNVAPIAVG
                     DGYEVNEDNVLVVPAAGVLGNDSDVPADTLSAVLISGPANGTLTLNADGSFSYTPALD
                     FNGSDSFTYSAHDEDGGASLSATVEILVHSVNDAPTVVLGPNLLVDEDDGAQEFAGWA
                     SAFLAGPADEAGQTLTLEISNDFEDLFSEQPTLGFDGTLRFTPGADAFGTALVTVALS
                     DNGGTERGGVDTSMQTFTITVAPINDRPLATSADFGIDEDTPLSAQLAGFDADGDALT
                     FAQFGPGPVHGTLLLNPDGSFTYTPHANYFTPDADPFGGDTFGFRVSDGIISSLPAVV
                     KIQVAPVNDAPVANGESYDLVEDEPLDLTPFGPGLGVLANDVDVDGDTLAAILVDGPD
                     HGSLVLLANGVFVYTPAADFFGPDSFTYQARDPSNELSNVATVSLTVQNVNDAPVAGN
                     ANVVVDEDDVLEDTLLPLTDPDAGEEFTFTILDAPDHGVLVTHPDGSFSYTPAPNFFG
                     ADSFTFRANDGELDSNVGTVSITVNPVNDAPVLDAIDDTSVDEGTPLLVTAHATDVDG
                     PADLPVYSLDVAPEGAEINPATGLITWTPSEVQGPGSHSFVVRATDSSSPDLFDTEQF
                     MVTVNEVNRAPVIDAVPDFGGDEAIDELTLFTVDVNATDPDVPANTLSYSFDQAPEGA
                     EIDPLSGVITWTPGETQDGEHLFVVRATDNGTPSMSDTEEFSVTVNEVNLPPVLNAIG
                     DRTVSEGELLTFTVGATDPDVPANTLAFTATGLPAGATFDPATGVFSWMPSEAQGPAT
                     YNGITFEVADLKGLFDSETISITVLEDASLDAGPQADDGNADDFRVVRNGANVEGYLN
                     DLLVFNRSAASLAGFPLTVTGSGDDDTLTVDFSGGDPIPPLGLAYAGGDSDRPDENDE
                     LVLTGTSPSSVVQFEASGPSSGTVTVDGSTITYTGLEPITLSAAAALEFGFQNGDDVI
                     TLTIGETTSTLSSPSSETITFVNPADSVTILARGGDDTIVVVNNAAGDPDFEIVIDGG
                     SGANTVTSDVPVQTALAGTDGDDVIGIAQTGADIVVTVNGATTTLENAASVRVDGRAG
                     DDVIDASDVHSIAVVLLGGEGNDQLTGGSANDTLLGGPGDDVLAGRGGVDVVDGGEGN
                     DTAVVQRVTPIAYWSLNETSGSGAADSAGTPQNGTFFGSNPDHDDAGPPLSAAPFGAQ
                     TGADFHDTTREYIAVQHSAEFEVPQGTVQLWFKTRDADDRQALFAKDRDGNGAGHLLI
                     WIDDHDLKVRLQTGTTSHTITANNVVSSNVWYQMTFTFGPAGMKLYLNGTQVGTNAYT
                     GGLTANQQPIVIGGSNATHRNGTSDLSRLRITDPFDGWIDEVAFYGAALSPAEIAQTK
                     AKGAMAVTAPADANDTFVSIENTVFADDPHAGDAAASTLQGTVFTDTALTNVATFFGL
                     NGRVDVGTFDGRAPCEPRAGSGGWASVLKQGVDFIKQKLHSASDERGDAKQESKQAKD
                     DWVVVAKSEAKAKDAIEKDRKGDAKIDWKGALASLGAPLFSRGGGHSRPAQPNIAEFN
                     HQPGKVKR"
BASE COUNT         2411 a         5911 c         6126 g         2796 t
ORIGIN      
        1 agtttatcta gctattcgcc tcgcccggag aaggcgagga tgtctcccag atagtcgtcg
       61 agatcgagca ggtcgcggcg cgcgcgcgag tgcgcgcgct cggcgcgcca ctgcgcgagc
      121 ctgaacaccg ccgcgccgag cgccgccggc acgccggcgc cgcgcgcgaa gccgtcgtgg
      181 acgagcggcg cgaacgattt caccaggtcg tcctcgagcg cgacgatggc ttcgtaggtc
      241 cccgggtcgc cctgccggcc gcagcgcccg aacagctgcc ggtcgatgcg cgccgcgtcg
      301 tgcagctcgg tggcgatgac gtgcagcccg ccgagctcgg gcacgccctc gccgagccgg
      361 atgtcggtgc cgcgccccgc catgttggtc gccacggtga cgcagccgcg ctcgccggcg
      421 cgcgccacga tctcggcctc gtcgcggtcc tggcgggcgt tcagcagcct gaaggaaata
      481 ttcctttcct gcaggagctt tgccaggtgc tcggatgcct tcacagagcg ggtgcccacc
      541 agcacgggct gcccccgcgc gctgcgctcg gcgatcgcct cgaccacggt ctgccacttc
      601 gccgcggcgg agccgaagta gcgcgaggag cggcggctgc gccgggtcgg gcggttggtc
      661 ggaacgcgcg cgacgcgcag ccggtacacc gcgcgcagct cgcccgcgat ctcggccgcg
      721 gtcccggtca tgccgcagag gtgcaggtag cgccggaaga agcgctggta gctgatgcgc
      781 gcgagcgtct cgcgcaccgg ggtcacctcc acgccctcct tgatctcgat cagctggtgc
      841 aggccgcgct cccacgagcg gtcgggcatc aggcgcccgg tgtactcgtc gacgatctgc
      901 accttgccgt cgcgcaccag gtagtgcttg tcgcgctgga agacatgcag cgcggtgagc
      961 gcctggcgca cgagctgctc gcgccggcgc ggccccttcc acacgccgcc aaggtccttg
     1021 ccggcgcgag cgagcttgcg ctcgcccgct tcggtaatct cgacgccctc ctcgccgagg
     1081 ctaaagtccg gctcgccgag cgtgccggcg atctcgagcg cctcgcggtg cagctgctcg
     1141 ctgccggtgc ggtccgagcc gcccgacagg atgagcggcg tgcgcgcctc gtccaccagc
     1201 acgctgtcgg cctcgtcgac gatggcgaac tgcagcccgc gcagcagcag ccgcccgccg
     1261 ccggcatttc cgtcccccgg gccgcccgcg agcgccccca ggcgcagcgc gatcgggtgc
     1321 ggcttgccgc ccagcaccag gcggtcgcgc aggtagtcga acgcgacctc cttgttgctg
     1381 cagtaggcga cgtcgcaggc gtacgcggcg cgccgctcgg ccggctgctg gccctgcttc
     1441 acgcagccga ccgacaggcc gagaagccgg tacaccgggc ccatctgcgt cgcgtcgcgc
     1501 tcggcgagat agtcgttcac cgtcacgacg tgcaccgcac gccccgcgag cgccgcggtc
     1561 gccgcggtga gggtcgcggt gagcgtcttg ccttcgccgg tgtccatctc ggccaccatg
     1621 ccgcgcagca tcgcccagcc gccgatcagc tggacgtcga agtggcgcag cccggtcgtg
     1681 cgcaccgaag cctcgcgtac cagcgcgaac gcctctgcgg cgagcgcgag gtcatcgaag
     1741 ccccgccgcc gcagctgcgg ccgcagcgcc gcggcacgcg cgcgcagctc agcgtccgag
     1801 gccgaagttg tttgttctcg atacccttcg actttctgaa taatcctgga gagctggcgc
     1861 gtgcccgact cggcgcgccc cgcgaggtag cgcagctcgg tgagcaggcg ctcgaacttg
     1921 ccggcgggag cgtcgttgcg ctcgggatag ccgcgcgcgt cgaaaaccgt ggtgctcaca
     1981 ccgtgaactg ccgcatgaag agctgccgca cgctgcggta gaggcgcccc gcgagcggct
     2041 cgccgccgtg ctcgaagcgc acgtagacgt gctcgccgag cgtgagcgtg ggtgtctgcg
     2101 gcagctcgag ctcgaacgcg aaccaggggt cgagcgtctt cggctccttg ccgctcgggt
     2161 cgagcgcggc gccgccgccc ccggcgctcg agagcgcgag gttggagacg cggttgctcg
     2221 ccgccggaac ctcgcgcacg agccgcgcct cgtaggtgtc gtagagccgg ccggccactt
     2281 tcgcctcgat gcgcagcgtg cggctgcgca ccaggtcgac gtcgtcctgc gacacgagca
     2341 cccgcgcggt ggcggccttg gcgggcagca cgtagcccac ctgctcgccg cggcgcagga
     2401 agcgctcggg caggtcggcg gcggcgtgcg ccagcacgaa ggtgccggcg accgggctcg
     2461 agatggtgag ctcggcgagg cgcgtgcgca cctcctcgag ctggcgccgg gccgcttcca
     2521 gctcgtccag cgtcatcgcc cagcgcacgc ggttctccag gcgcagcgcg ttggcgcgcg
     2581 cctcgagcag ccgcacctcg gcttcgagca cgcgctcgcg cagcgcgagc atcgggtcct
     2641 cggtgatcac gagctcggtg cccgcgccga cgatcgagcc gggcgcggcc acgatcttgc
     2701 gcacgaagcc ctcggctccg gcgcgcaggt ggctctcctc gggaagcgcg atcacgccct
     2761 cggcgcgcgt ccacagcggc accggcagca cgaacaaggc tccataaagg agggccagcg
     2821 caagcgcggt ggtcgccagg gcgcgcggcc tgcggcgcct cagccgggga tgcatcagca
     2881 ggtagccgac gcccttcagg accgggagca cgaccgcggt ggcgagcgcc cacagcgcga
     2941 gcagcacgcc gaccaggaga tattcgctcg cgatgaagag cgcgatcgcg caaatcacga
     3001 acgtgcgata gaggaacgaa agcaccgcgt agaagccgag ccaggcgcgc tccgcggcgg
     3061 gcgtcggctc ctcgtccagg ttcacgccga acagccgccg ctccaccagg cggccgagat
     3121 actggttcgc ccgcacgcgc aggttgggga tctgcacgag atccgccagc atgtagtagc
     3181 cgtcgtagcg cagcagcgga ttcgcgttga acacgatcgt cgagatcccg gcgatcagca
     3241 tcacgtcgta cagcacggcg cgtgcgaggc cgggctcggc ttcggcccac agctggatcg
     3301 cgatcgaggc gaggaacacc tccaccagca tgccggccgc tccgaccacg acgcggcggc
     3361 gcgcccgcgg gaacgccgag gccgccgagg cgtcgacgta cgggatcggc atcaggacga
     3421 gcagcatgat gcccatctcg tgcacctcgc cgccccaggc cttcaccgcg caggcgtgcc
     3481 cgagctcgtg caggagcttc accaccggga aggtgagcca caggatgagc aggttctgcg
     3541 gcgcgaggat ctgcgaggtg acgtcggcgg tgagctcgtc ccagtgcccc gcggcggtgg
     3601 cgatgccgaa gccgaccacg accgcccaga cggcgaggcc gacccagccg aaccacgggc
     3661 cgtaccaggg cagccagcgc tcgaggaagc ggtccgggtc gaccagcggg aagcggatcg
     3721 caagcggcga gagcaggttc tgcagccagg tcttgcgctt gcccttgcgc gcgcggcgcg
     3781 ccagctcggt gaggtcgggc atcgcctcgg tctgcagcac ttccgccgcg tgcagctgcc
     3841 cgaggagccg gatcacctcg tcctgggtcg gcgcgtcgtc gccgaagcgc tcggtggccg
     3901 cttcccagat ctcgtgcacc gtgcgccgcc cgtcgagcag cccgacgatg tagtaggccg
     3961 ccgggttgaa gcggtgcatg cgccgcgaga cgcggtcctc gagcacgtac cagagctcgc
     4021 cgcggtagtg gtggcggtgg atgcgcgcgt ggccgcgcag ccgcggccgg agctgcgcga
     4081 cccggtacca cgatgggcta tgaagatcag acattcaagg aagccaggac cagagcttga
     4141 gctgcagcca gtcggtgaag ctgcgcgtcc agatccacac caggcgccgc tcctcgacgt
     4201 ggatcttgcc gacgccggtc atgccggggc gcagccggcc tggctcgccg tcgaggcgcg
     4261 cctccacccg gaacacgttg cgcccctcct gcgccacgtt gaccggcacg atcgaggtga
     4321 cggtgaaggg cagcgcctcg cccggcatcg cggtgaccgc gagctcgccg cgctggccga
     4381 gcgcgacgtg cgccatgtcg cgctcgtcca cccgcagcac gatgcggtag ccgtcgagcg
     4441 gcgccacctc gaacagcacc tggccgcgct ccaccggcga gccgaggctc tgcgaaagat
     4501 cgccgctcac gatcagcccg tcgaccggcg cggtcatcac cgagcgcgcg agctgctcct
     4561 cgagcagcgc gagctgcgcc tcgttctggt cgagctgcgc ggcgaggatc gcggtctgcg
     4621 cgcggtcgcg cttcgccatc gcctcgcggt gctggcgctc gacctgctcg cgctgcgcca
     4681 cgagcttgat tctctccagc tgcagctcgc ggtcctcgaa gcggccgatc ggctgtccgg
     4741 ccttcacccg gtcgccggcg cgcagcggcg cctccttgac gtagccgttg aacggcgcgg
     4801 tgatcgcgcg ctgcatccgg ccctcgaccg tgacgtcggc cgagacccgg aacgtgccgg
     4861 tggcgagcgc gaggaacgcc gccgccgcgg cggtgaggac cacggcgagc ttgaagccgg
     4921 gatggcgcgg gccggcgagc ttcttccaca gatccgccgt gctgcgcccg gcgtgcagcg
     4981 cgaggttcgc ctcggcgcgg cgcttgagct cgacgatcgg tccggcgacc gcggcgagcg
     5041 cctcgccgag ctgcaggctc tccgggtcga agcggtaagg cgcggcgcgc tcgaggctga
     5101 gcgcgccgac gacctcgccg tcgcacacca gcgggaaggt ggccgcggcg ccggcccccg
     5161 attcgcgcag cagcacgtcg tgcgcatgcg ccaccaccgg aaggtcgtcg cgcggcggcg
     5221 ggctgaccac gagctcgccc tggtcgaccg cctcggccat cgcgttctcg accgcgcgca
     5281 gcaggttggc gcgctcgtcg aactgcgcgg agtgcgacac gccgatcacc ttgatcgcgc
     5341 gccctttcag caggcccacc gcggcgcgct cgcagcccag gcgcgtcgcc acctcggtgg
     5401 cgaaggccgc ggccgactcg cgcagcgcgc cctgctcgag cagcgtcgcc accaggtcga
     5461 gcgcgagctt gagcctgagc tgcgccgcgg cggccgggtc ggcgtggcgc cgcagcagca
     5521 cctccagcca gcccgagccc cactgcagcg agcgcatcgc gagatgcagc tgcggctcgg
     5581 ggcgccagtc catctccagg ccgaccgcgc cgcgcacttc cccgtcgagg cgcaccggat
     5641 agccgagctg ataccgcggc tcgctttgcg aaggcccgag cggcaggacc acgccgcgcg
     5701 cctccttgag cgtgcgctcg gcgatctcgg cgaggtgcgc gcggtcgcgt ggcgcgtccg
     5761 gatggaacgc gaccgggacg aagtcgcgcg ccccgggcgt ctgcagcacg accacgccgt
     5821 cggacacgcc gccgatcgcg tcgcactgga tcgcgagcca gctcgagcag aactcctcgt
     5881 gcgactgagc ctgggcgaag cgcgcccaag cctggtcggc gccgcgccgg ctcgccagct
     5941 cggcgacgct ggcgaggcgg cggttctcct ggatctctgg ctgcaccggg ctcatcgaat
     6001 gtctcgggac cccggctgcg cgacgcgacg acctacttct tcgcgtcgcc gcccggcagg
     6061 tcgatgttga gcgtgccgaa gcgcttctcg tactcgttga cgacgccctg cagcagcatg
     6121 ctcagccgct tggcggcgaa cgggctcacc acgatgcggt cggtgagatg gatcgtgact
     6181 tccttctgcc cgcggttcca cgcctggttc atgccgaaca gcaggacgac ctcctcgcgc
     6241 gtgctggtga cgttgcacac gttggcgtac gagctcttca tccccgagtc gtcccacttg
     6301 atgcgggtcc cctccagggc ggctctcgcg tccgtccccg ccgccgtgtc tgcgcctttt
     6361 gtagccatgg tattgccgct cccttttcga tctcagttca gtgttggatg ctgcaatgcc
     6421 atcttaaccc ggggcgggcc gggtttgacg ccggtcaacg cttgaccttg cccggctggt
     6481 ggttgaactc ggcgatattg ggctgcgccg gcctggaatg cccgccgccg cgggagaaga
     6541 gcggcgcgcc gaggctggcg agggcgccct tccaatcgat cttcgcgtcg cccttacgat
     6601 ctttttcaat cgcatccttg gctttcgcct ccgacttggc cacgacgacc cagtcgtcct
     6661 tggcctgctt gctctcctgc ttggcgtcgc cgcgctcgtc cgacgccgaa tgcagcttct
     6721 gcttgatgaa gtccacgccc tgcttcagca cgctcgccca gccgccggag ccggcgcgcg
     6781 gctcgcaagg cgcgcgcccg tcgaaggtgc cgacgtcgac gcggccattg agcccgaaga
     6841 acgtggcgac gttggtgaga gcggtatcgg tgaacaccgt cccctgcagc gtgctcgctg
     6901 cggcgtcgcc ggcgtgcggg tcgtcggcga acacggtgtt ctcgatcgag acgaacgtgt
     6961 cgttcgcgtc cgcgggcgcg gtgaccgcca tggcgccttt cgctttcgtc tgcgcgatct
     7021 ccgccgggct gagcgctgcg ccgtagaacg ccacctcgtc gatccagccg tcgaacggat
     7081 cggtgatccg caggcgcgac agatcgctgg tgccgttgcg gtgggtcgca ttgctgccgc
     7141 cgatcacgat cggctgctgg ttcgcggtca gcccgccggt atacgcgttc gtgcccacct
     7201 gggtgccgtt caggtagagc ttcattcccg ccgggccgaa cgtgaacgtc atctggtacc
     7261 agacgttcga cgacaccacg ttgttcgcgg tgatggtatg gctcgtggtg ccggtctgga
     7321 gcctgacctt caggtcgtgg tcgtcgatcc agatcagcag atggccggcg ccgttgccgt
     7381 cgcggtcctt ggcgaacagc gcctgcctgt cgtcggcgtc gcgcgtcttg aaccagagct
     7441 ggaccgtacc ctgcggcact tcgaactcgg cgctgtgctg cacggcgatg tactcgcgcg
     7501 tcgtatcgtg gaagtcggcg ccggtctgcg cgccgaacgg cgcggccgag agcggcgggc
     7561 cggcgtcgtc gtggtccggg ttcgagccga agaaggtgcc gttctgcggc gtgcccgcgc
     7621 tgtcggccgc gcccgagccc gaggtctcgt tcaggctcca gtaggcgatc ggcgtgacgc
     7681 gctgcacgac cgcggtgtcg ttgccctcgc cgccgtcgac cacatcgacc ccgccgcggc
     7741 cggcgagcac gtcgtcgccc gggccgccga gcagcgtgtc gttggccgag ccgccggtga
     7801 gctgatcgtt gccctcgccg ccgagcagca cgaccgcgat gctgtgcacg tcggaagcat
     7861 cgatcacgtc gtcgccggcg cggccgtcga cgcgcacgga ggcggcgttc tcgagcgtgg
     7921 tggtggcgcc gttcaccgtc acgacgatgt ccgcgccggt ctgggcgatg ccgatgacgt
     7981 cgtcgccgtc ggtgccggcg agcgcggtct gcaccgggac gtcggacgtc accgtgttcg
     8041 cgccgctgcc gccgtcgatg acgatctcga agtccgggtc gcccgcggcg ttgttcacca
     8101 ccacgatggt gtcgtcgccg ccgcgcgcca ggatggtgac gctgtcggcc gggttgacga
     8161 acgtgatcgt ctcgctgctc ggactggaga gcgtcgaggt ggtctcgccg atggtgaggg
     8221 tgatcacgtc gtcgccgttc tggaatccga actcgagcgc agccgcggcg gacagcgtga
     8281 tcggctccag gccggtatag gtgatcgtgg aaccgtcgac cgtcaccgtg ccggagctcg
     8341 ggcccgaggc ctcgaactgc accaccgagc tcggcgaggt gccggtcagc accagctcgt
     8401 cgttctcgtc gggccggtcg gagtcgccgc cggcgtaggc gaggccgagc ggcgggatcg
     8461 ggtcgccgcc ggagaagtcg accgtcagcg tgtcgtcgtc gcccgagccg gtgaccgtga
     8521 gcgggaagcc ggcgagcgaa gcggccgagc ggttgaacac cagcaggtcg ttcaggtagc
     8581 cctcgacgtt cgcgccgttg cgcacgacgc ggaagtcgtc ggcgttgccg tcgtccgcct
     8641 gcgggccggc gtcgaggctc gcgtcctcga gcaccgtgat cgagatggtc tcgctgtcga
     8701 acaacccctt caggtccgcg acctcgaagg tgatgccgtt gtaggtcgcc ggcccttgcg
     8761 cctcgctcgg catccagctg aagaccccgg tcgccggatc gaacgtcgcg cccgcgggca
     8821 ggccggtcgc ggtgaacgcg agcgtgttgg ccgggacatc cggatcggtc gcgccgaccg
     8881 tgaaggtcag cagctcgcct tcgctgaccg tccggtcgcc gattgcattg agcaccggcg
     8941 gcaggttcac ttcgttcacc gtgacgctga actcctcggt gtcggacatg ctcggcgtgc
     9001 cgttatcggt cgcccgcacc acgaacaggt gctcgccgtc ctgggtctcg cccggcgtcc
     9061 aggtgatcac gccgctcagc gggtcgatct cggctccctc gggcgcctgg tcgaacgagt
     9121 agctcagcgt gttcgccgga acgtccggat cggtcgcgtt cacgtccacc gtgaacagcg
     9181 tcagctcgtc gatcgcctcg tccccgccga agtccggcac cgcgtcgatc accggcgcgc
     9241 ggttgacttc gttcacggtg accatgaact gctcggtgtc gaacagatcc ggcgagctgc
     9301 tgtcggtcgc gcgcaccacg aagctgtggc tgcccgggcc ctgcacttcg ctcggcgtcc
     9361 aggtaatgag gccggtagcc gggttgatct cggctccctc gggagcgacg tcgagggagt
     9421 agaccggcag gtcggcggga ccgtcgacat cggtcgcatg cgccgtcacc aggagcggcg
     9481 tcccctcgtc cacgctggtg tcgtcgatcg cgtcgagaac cggcgcgtcg ttcaccgggt
     9541 tgacggtgat cgagaccgta cccacgttgg agtcgagctc gccgtcgttg gcgcggaacg
     9601 tgaagctgtc ggcgccgaag aagttcggcg ccggcgtgta gctgaagctg ccgtcggggt
     9661 gggtcacgag cacgccgtgg tccggcgcgt cgaggatcgt gaacgtgaac tcctcgcccg
     9721 cgtccggatc ggtgagcggc agcagcgtgt cctccaagac atcgtcctcg tcgaccacca
     9781 cgtttgcgtt gccggcgacc ggcgcgtcgt tgacgttctg caccgtcagc gagacggtcg
     9841 ccacgttcga caattcgttg gagggatcgc gtgcctgata ggtgaagctg tccgggccga
     9901 agaagtccgc ggccggcgtg tacacgaaga ctccgtttgc caggaggacg aggctgccgt
     9961 ggtccgggcc gtccaccagg atcgcggcca gcgtgtcgcc gtcgacgtcg acgtcgttcg
    10021 ccagcacgcc gagtcccggc ccgaagggcg tgaggtcgag cggctcgtcc tcgaccaggt
    10081 cgtagctctc gccgttcgcg accggcgcgt cgttgaccgg tgccacctgg attttcacca
    10141 ccgccggcag cgagctgatg atgccgtcgg acacgcggaa cccgaacgtg tcgccgccga
    10201 aggggtccgc gtccggcgtg aagtagttcg cgtgcggcgt gtaggtgaag ctgccgtcgg
    10261 ggttgagcaa gagcgtgccg tgcaccgggc cggggccgaa ctgggcgaag gtgagcgcgt
    10321 cgccgtcggc gtcgaagccg gcaagctgcg cgctgagcgg cgtgtcctcg tcgatgccga
    10381 agtccgcgga ggtcgctagc ggccggtcgt tgatcggcgc gacggtgatc gtgaaggtct
    10441 gcatcgaggt gtcgaccccg ccgcgctcgg tgccgccgtt gtcgctgagc gccacggtca
    10501 cgagcgcggt gccgaaggcg tctgccccgg gcgtgaagcg cagcgtgccg tcgaagccga
    10561 gcgtcggctg ctcgctgaag aggtcctcga agtcgttcga gatctcgagc gtgagcgtct
    10621 ggcccgcctc gtccgccggc ccagccaaga acgcgctcgc ccagccggcg aattcctgcg
    10681 caccgtcgtc ctcgtcgacc agcaggttcg gcccgaggac gactgtgggt gcgtcgttga
    10741 ccgaatgcac caggatctcg acggtggcgg acaaggacgc gccaccgtcc tcgtcgtgcg
    10801 ccgagtaggt gaagctgtcg gagccgttga agtcgagcgc cggcgtgtag ctgaacgacc
    10861 cgtcggcgtt cagggtcagc gtgccgttcg ccgggccgga gatcagcacc gccgacagcg
    10921 tatcggccgg gacgtcgctg tcgttgccga gcacgccggc cgccggcacg acgagcacgt
    10981 tgtcctcgtt cacctcgtac ccgtcgccga ccgcgatcgg agcgacgttg ttgacggtca
    11041 cctcccaggt gcccgtgccc gtggcgcctt cgtcatcggt gacggcgatg gtgaccgtga
    11101 agtcgccgtt gtcggcgtag gtgtgcgaga cactgaccga tccggtgaca ttggggaaga
    11161 gcaccggcag cgtgccgtcg ccgaagtcga ccagcgccgt gtgcgtatcg ccgctgcccg
    11221 ggtcggtgaa cgggatcagc aatgcgaggg gcaccccttc gtcgacgatc gctgcgccgt
    11281 cggtgacgac cggcggtgcg ttgttgatcg tgacgaacag gccgtcgctg tcctcgccac
    11341 cgtcgtcgtc ggtcaccgtg agggtgacga gatacggccc gttgtcggcg tacacgtgtg
    11401 cgccggtgac ggtcttcgtg gccgcatcga ccgcgcccat cgtgaccatg ccgtcgcccc
    11461 agtcgatctc ggccgtgtgg gtgtcgagga agcccggatc ggtgaaggtg gcgctgacca
    11521 tcaccgtgtc gccttcgaac gcttcgacgt cggcgccggc ggtgacctcg ggcgccacgt
    11581 tgagcacctt gacgagcacc gtgtcggcgc ccgattcgtc gtcgtcgtcg gtgacggtga
    11641 gcgtgaccgt gaattgcccg gcgccgctgt cgtcgccgta gaagtggctc gccgcggccg
    11701 tgcccgacat gccggcggtc gatcccggcg gcccgaaggg cgtctcggtg accgtgccag
    11761 cgctcgtggt cccgtcgccc cagttgacgc tggcggtatg cgtgtcgagg gtgcccttgt
    11821 cgttgaacga cgcgccgctc aggctgaact cgtcgccctc gaagatcgtg gtgcccgtgc
    11881 cggcctccac cgtcggcgga tcgttgacca cgtcgacgat catggtcagc gtctcggtcg
    11941 cgatgccgcc gtcgtcgtcg tgaagcacga aggtcacgat gtattcgccg tcgtcggcgt
    12001 acacatggct gccgttcagg gtaccggtca ggccgccgag cgatcccggc gggccgaacg
    12061 gcgcggaagc aatgaccagg tcctcggtcg cggtgccgtc gccccagttc accgtgccgg
    12121 tgtgcgtgtc gagcgtgccc gggtcgagga acgtgacgtc cttgagcgat atgagctcgc
    12181 cttcgcgccc agcgtgggcg gtcggccgga tcaccggcgc gacgttggtg atcgtgatgt
    12241 caaaggaatc ggagtgctcg ccgccgttgt cgtccgtcac ggtgacgatg acggtgtaca
    12301 cgccgtcgtc cgcgtagacg tggctgccga gtaccaggcc ggtgagcccg aactcgctgc
    12361 ccgggggccc gaacggaacc tcgctgacca gtcccgtttc ggccgccgtg ccatcgcccc
    12421 agtcgatcgt ggcgctgtgc gtatccagcg tgcctgagtc agtgaagcgg acggtcggcg
    12481 gcaacggcac atcctcgccg atgacgacgt tgtcgccgat cacgatcagc gggctgagcg
    12541 acaccagcgc gccttcatcg gccgtgcggt cggggccagc atccacgacc ggggcgacgt
    12601 tgtcgaccgt gaccacgagg gtgtcgctgg tggtagcgcc gtcgtcgtcg gtgaccgaga
    12661 ccatcacggt gtagacgccg tcgtcggcat agacatgcgt cgccgggatc gttcccgcgc
    12721 cgttcgtctc cgtcagcgac tcgaagatcg tagtcgtccc gtcgccccag gcgatcaccg
    12781 ccgtgtgcgt gtcgttagtg ccggcatcgt cgaacgcggg ctcgatcgag acgatctcgc
    12841 cttcgaacac ggtctgggcg gtccccgcct cgacctcggg cgcgacgttg agcgaggtga
    12901 cgatcacggt gtccggatga gaggtgagac cgcggaaggt atcggtcacc gtcagctgga
    12961 cggtgtacgt accgttgtcg aacggcacga agctgaacgt cgcttcatcg ccgtcggcga
    13021 tgatctggcc gttgtcggcc tgcacttccc agaggtacgt aaagactgcg ccgggcgagc
    13081 cgacgaccgt cggcatgaaa ctgacctcat caccctcgtc gacctcgaag tcttcgccca
    13141 ggtcgaaatc ggctatgagc tggttgccga agtccacgcc ctcgacgatc tggccggcgt
    13201 cggtgaacag caccggcagc acgccgatca cgccggccgg ggtgctggtc gcgccggtgg
    13261 tcggggtcca gccggggaac ggaacctcgg cgaccaggta ggagcgattc gtaccctgcg
    13321 cgaacccgct gccgccgaag ccgagcggat ccgggccgat cggcagctgg aacatttcga
    13381 catgctcggc gccgttgccg aaggcggtcg tggtgacgct ctggccgaag gtgtcggaga
    13441 agtgcagctg gaccgtgcgc gtgtcgccga cgcccgtggt ggtgaacttc aggcggccgt
    13501 ccgctacgcc gacggtgacg agcggcccga ccaccgggtc gccgttgacg agcgcctcga
    13561 ggtccgcggc cagatcgaag atgtcgtcgt tgtccagcgt cttgtcgtgc atgagctgga
    13621 agtagcccgt gaggccgtcg acgctcagca ggaactcggt ggtccgcgtg gtctcggtct
    13681 cgatagtcgc ttcgccggca gcgacgatct gtttgaggcc cacgtcggcc gtgatgtcgg
    13741 tctgtgcgac gccgagctcg cccaggctgc ccggggtctg atcctcgctg aaagccgcga
    13801 acccgaacgc gccgcccgtg acttcatcca gacgggtgtt gatgatcggc gcaccggcgc
    13861 gctcgagggt ttcgatcacc gtctggtgcg tggaagccgc cacctggaac gcgtcgaacc
    13921 cgccggcgag ctggaagaac agcacgccgt cctcgaagtg cgccgagagg aggtggctca
    13981 ccttctggag ggcgaacgcg aggttgaagt cgtccctgag gtccgcgccc gtgaggttgt
    14041 cggaggtctg ctcgggcgtc aggatgaccg tggtggagaa gcccggcggc accggctcca
    14101 gggtacccgc atccacgcgc agcggcgcct cgacgcccgt gccgagcgtg ccgtggtttg
    14161 cgttgccgga gctgtcgatg gctacgccgc cgctctcgtc gaactgccag taaccggcca
    14221 ggccgaccgc cggcgcggca atgccgtccg cgaaatccgc cttgatctcg tcctgcgtgc
    14281 gcggcttgtt ccagatcgcc acgttgtcga tgatcccgtc gaagcccgcg aagttcgtct
    14341 cgagcgtgtc gccgatgagc agcgggttgg tggtgacggc agcaggggtc ttgagcaccg
    14401 tgctgctcaa gacctccgtt ccgttcagat aggccttcac cggtcctctg gaggggtcgt
    14461 cggggtcggt agagtcgcgg tcgatcaccg tcgcgaactc gtaccactgg ccgggttgca
    14521 gcacgacgtt gctcaggaac caggactgct gcccgctttg cgcgtcccac gtatcgaaga
    14581 acagggtgcc cgtgttctga ttgatgaaca tcgagtacgt gcggaacagc gggcttgcgc
    14641 ttgtcttgtg caccagcgcc ctgaactggt tcgtagcgtc gaagtcatag cggaagcgca
    14701 cttcgaccgt cgaggagccg gcgagcgcgg tgctcgcgtt gtgcgcgacg gtcacgcggt
    14761 cgccgccgtc gaacgagagc gcgtccggca ggctctgcgc ggtgaactgg aacgtcgcct
    14821 cggtgcggtc gacgatctcg gtctgcgcgg tcgtggtcgc gatcgtctgc gtcgcgccgc
    14881 cgtggaattc ggcgggcgcc gcggcgacga gctcggccgg gccgtgcggg accggatcga
    14941 cgacctcgag gccgcggaag ccgtactcgc cgttgacgtc ggtataggtg acgggctctc
    15001 tctcgtcgag cagcccgttg gcgttcaggt cgaggaacag cttcacgccg tcgatgcccg
    15061 gctcgttcgg gtcgcgcacg ccgtcgccgt cgatgtcgtt gaacttggtg ccgtagatcg
    15121 cctgcgcgct gttgccgccg ctcgtgtact cgaaggtgac ggtgtggtgg cggccgtggg
    15181 ggtcgcccgc cgcgccgagg ccgtcgaacg cggtcagcgt gatcaccacg gtgccctcga
    15241 agcccggcgc ctcggtgatc agcaggttgc tctcgtcgag catctcggcc tgcacctgcg
    15301 gcgtgtcgct caccacgtgc aggtagatcg gatcgccgag ctggtcgcgc acctcgatgc
    15361 ggatcgggcc gtggctgcgc accgcgccgc cagaaggcgt gccatggttc ccgttaccgc
    15421 tgtagtcaac gacgctgcca aacgccgtgc tggtggtctg gtcgtcgaag gtccagtagc
    15481 cggcgagccc cgcctcgtcg ccgtcgagga tccggttcat cgcgtcgcgg atctcggtct
    15541 ggctgcgtac ggcggtccac acgcgcactt cgtcgatctg gccctggaag tgctgcgtga
    15601 aggactcgcg gtcgccgatc tgcagggtgt tctggccggt gtgcacgtcg ccgatctcgc
    15661 cggcgccgtc gaagaagtgc accagctcgc cgtcggcgta ggtgcgcacg tcgccgttgt
    15721 cgtagaccac cgcgatgtgg gtccactcgt tcagcttcgc gacgtagccg gtgttgatcc
    15781 agacccagcc ggggttggag ttggcgaacg cccagcggat cgtgccgtcg gagaagcgcg
    15841 cgacctcgta ctcgccttcc ttgttgacga tgatgccgtc ctggcccggg ccggtgggat
    15901 tgatccaggc ttcgatcgtg agctcgccgt cggcctgcag cgaagccgag ttggcgacgg
    15961 tgatgcgcga gctggtgccg ttgaatgtga acgcgttggg cgtgcccggc tggttggcga
    16021 tctgcgcgat ctcgggcgcc tcgttgggcg tgaactcgac gtccggcccg acgccctggc
    16081 cggcggcgaa gccgagctgc tcggcgcctt cgacgcgcag cgaggcgatg tcggtgtcgg
    16141 tcacgtcgaa tgagaggcgc tcgccgacga cctgaaggac gatcgcgtcg tcctcgaggc
    16201 cgaggtcgtc cagcgcatcg gccagcagcg tgttgagctg cgcggcgagg ccggcgaggt
    16261 cgctgttcgc ctggctctgg atcgcgctga gctcgacggt gccggagagc agcgtgccgt
    16321 cgttgagccc gaccgtgaac ttcagcgtga ggtcgttgac gaagtcgccg tccaccggcc
    16381 cggggttgac cgcggtcagg ccgagcgtgg aagcgcgcga ctcgagcact tcgatgaaga
    16441 tgtcgtgcga gcgctggtcg agcgggttgt cgctgagcgt gatcaggcgc agccccggca
    16501 gcgaggtgtc cacgcgccgc aggatcacgc cgtcggccgc gacgatcccg ccgcttgcat
    16561 cggtgatcac gacctcgatc gccgggctct cgggcgtggt ggtgtcgggc gtgacggtaa
    16621 cgatgcccag gctctcccag ctgcggccgg cgaactgcac gtcgtccggc gcgatgcgct
    16681 ggttcagcat caccgtgagt ccgggctcgc gcacgtagag cacttcgccg ttgttgagct
    16741 cctcgaccga agcggagaag tcgacgtcca cgccgtcgac ttcccgcgtg gcgaggatgc
    16801 tgaactgctg cgccgcctcc ggaatcagca gcgcgtagat cgcgccggcg gcgaagctcg
    16861 tcatcgggtc gtccgatccg tcgccgaaca cggggtcggc cgccgtgccg ccggtgcgga
    16921 acgcggcgcg ctcgccgacc gagccgatgc gcagggtcgg cgtatcgaac gccgcacccg
    16981 agatattggc cacgagcacc aggtcgtagc cgaggtagtt gtcgaccagc ccggcgatgc
    17041 cggtatcgac gcggatcacc gcgaggcccg cgccgtcggg cgcctcgtcg aacacatcat
    17101 cgccgagcag cgtcgccgca tcgagcgtga gcaggccgaa ggcttcggcg ccgcccgcga
    17161 ggcccgcgat gcgcggagtg aggatcgctc cgccgccgaa gtcgacgaac ggattgtcgg
    17221 tcagcaccgc gtttacgaag ccga
//
LOCUS       DASPFR010000148         2892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1509934, whole genome shotgun sequence.
ACCESSION   DASPFR010000148 DASPFR010000000
VERSION     DASPFR010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509934"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VD737_03690"
     CDS             <1..498
                     /locus_tag="VD737_03690"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HXW09690.1"
                     /translation="YDDTIKLFKDAGQSGSFFSKSYGYAVFPTIGKGGLGVGGAYGEG
                     RVYEKGKYVGDTKMTQLSIGFQAGGQGYSQMIFFQDKRAIDEFKSGNFEFGANANAVV
                     ITAAASGQAGTSGASAGASGGKKDATTAGEYHKGMAVFQIVKGGLMYEASVSGQKFSY
                     QPLGK"
     gene            816..2183
                     /locus_tag="VD737_03695"
     CDS             816..2183
                     /locus_tag="VD737_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXW09691.1"
                     /translation="MRAELDKPFTGDLDEMVKRRLVRIGVPYSRTHYFVDQGVQRGVA
                     YEYGKLMEDELNKRRKTGNMKVAFWFVTLPRDRLLPALVDGRVDMVIAALTVTPERQK
                     LVDFTKPTRENVSEIVVTGPGAPPINSVDDLSGQEVFVRKSSSYYQSLLSLNQRLKAA
                     GKSPVMIEEAPENLEDDDLLEMVNAGLIEATVVDDYIAKFWKEVFTDLVVHEGVAVRT
                     GGTLAVAIRKGSPQLAQGLNVIIDEYGLGTVFGNMMQKRYLVSTKFVKNSASEAERKK
                     FLAMVDLFKKYSDQYDVDYLLMAAQGFQESGLDQSVRSQVGAVGVMQVMPATAKDLNV
                     GDIRQLEPNIHAGIKYMRWMVDHYFKDEPMDALNKGLFAFASYNAGPGRIRQLRQEAA
                     KRGLDPNVWFGNVEQIASERIGRETVTYVSNIYKYYVAYRLVTEERERREAAKKGLGS
                     PQEVD"
     gene            2210..2509
                     /locus_tag="VD737_03700"
     CDS             2210..2509
                     /locus_tag="VD737_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09692.1"
                     /translation="MLRKTTMLAVVGALATASALVATTTSYAQTGGMERRGDRRDDRQ
                     GARDEKRECKAGDEKTRPECRQDKREGKQEDRHDGDDAETAPAPDPEAAKPESNG"
     gene            complement(2581..>2892)
                     /locus_tag="VD737_03705"
     CDS             complement(2581..>2892)
                     /locus_tag="VD737_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HXW09693.1"
                     /translation="IESTPAPMYGPGWGYRGWYGAPYGYGGTEVTTRRYNVGTLVMDV
                     IDREKRQAVFQGGIEGVVTKEMLANKQAAIDQAVAHIFSKYPFVAGQSAPVAPAGDEK
                     K"
BASE COUNT          571 a          900 c          981 g          440 t
ORIGIN      
        1 tatgacgaca ccatcaagct gttcaaggac gcgggccaga gcgggtcgtt cttcagcaag
       61 agctacggct acgcggtgtt cccgaccatc ggcaagggcg ggctcggggt cggcggcgca
      121 tacggcgagg gccgcgtcta cgagaaaggc aagtacgtcg gcgacaccaa gatgacccag
      181 ctgagcatcg gcttccaggc gggaggccag ggctacagcc agatgatctt tttccaggac
      241 aagcgcgcga tcgacgagtt caagtcggga aacttcgagt tcggtgccaa cgcgaacgcc
      301 gtcgtcatca ccgcggccgc ctccggccag gcgggcacaa gtggcgcatc ggcgggtgcg
      361 agcggcggca agaaggatgc gacgacggcc ggcgagtacc acaagggcat ggccgtgttc
      421 cagatcgtga agggcggcct gatgtacgaa gcctcggtgt cgggacagaa gttctcttac
      481 cagcccctcg gcaagtagac gtcgcgcgcg ttcgttcgtt tgcgcgattc cgccgctgca
      541 cggtccggcg cgcgaatttg atttcgtgac agatcgtcga gcgatgtcgt gtacgacgat
      601 gtgacggggg tcttatgcac gttgcaatgc agtccacccc agggttcggc cgcacttcgg
      661 ccattcttct tttcctggca ctctgcctag cgggctgttc cggcgggaag gagggacccc
      721 cggcggcggc accggcccca tcggccgcga cggcggtcgc cgagagcgaa gtgccgatgc
      781 agccgctgcc ttatgaaacg gccctgcccg atagcgtgcg ggccgaactg gacaagccgt
      841 tcaccgggga cctcgacgag atggtcaagc gccgtctcgt ccgcatcggc gtgccgtaca
      901 gtcgcacgca ctacttcgtc gaccagggcg tgcagcgcgg cgtggcctat gagtacggca
      961 agctcatgga ggacgagctg aacaagcgtc gcaagaccgg caacatgaag gtcgccttct
     1021 ggttcgtgac gctgccgcgc gaccggctgc tgccggcact ggtcgatggc cgggtggaca
     1081 tggtgatcgc ggccctgacc gtgactcccg agcggcagaa gctggtggac ttcacgaagc
     1141 cgacacgcga gaacgtcagc gagatcgtcg tgacggggcc cggtgcgcca ccgatcaact
     1201 ctgtcgacga tctctccggg caggaggtgt tcgtccgcaa gagcagctcc tattaccaga
     1261 gcctgctctc gctcaaccag cggctgaagg cggcgggcaa atcgccggtc atgatcgagg
     1321 aggccccgga gaacctcgag gacgacgacc tgctcgagat ggtcaacgcc ggcctgatcg
     1381 aggccacggt cgtcgacgac tatatcgcga agttctggaa ggaggtcttt acagacctgg
     1441 tcgtgcacga gggcgtggcc gtgcgcaccg gaggcaccct ggccgtcgcg atccgcaagg
     1501 gcagcccgca actggcccag ggactgaacg tcatcatcga cgagtacggc ctcggcacgg
     1561 tgttcggcaa catgatgcag aaacgctacc tggtcagcac gaagttcgtg aagaactccg
     1621 cttccgaggc cgagcgcaaa aaattcctgg ccatggtgga cctgttcaag aaatacagcg
     1681 accagtacga cgtggactac ctgctgatgg cggcgcaggg gttccaggag tccggcctcg
     1741 accagtcggt caggagccag gtgggcgccg tgggcgtgat gcaggtgatg ccggcgaccg
     1801 cgaaggacct gaacgtgggg gacatccgcc agctcgagcc caacatccac gcgggcatca
     1861 agtacatgcg ctggatggtg gaccactact tcaaggacga gccgatggac gcgctcaaca
     1921 agggcctgtt tgcattcgcg tcgtacaacg caggcccggg gcgcatccgt cagctgcggc
     1981 aggaagcggc gaaacggggc ctcgacccca acgtctggtt cggtaacgtc gagcagatcg
     2041 cgtccgagcg catcggtcgt gaaacggtca cgtacgtgag caacatctac aagtactacg
     2101 tggcctatcg actggtcacc gaagaacgtg agcggcgcga ggcagccaaa aagggtctcg
     2161 ggagcccgca ggaagtcgac tgacacacac aaacgaggag gagcgcaaca tgctacggaa
     2221 gacgacgatg ctggcggtgg tcggtgcgct ggcgacggcc agcgcgctgg tcgctacgac
     2281 gacgagctac gcccagaccg gcggcatgga acggcgcggc gaccggcgcg acgaccggca
     2341 gggcgcgcgc gacgagaagc gcgaatgcaa ggccggcgac gaaaagacac gtcccgagtg
     2401 ccggcaggac aagcgcgagg gcaaacagga agaccgccac gacggcgacg atgcagagac
     2461 tgcgccggca cccgatcccg aagccgcgaa gcccgagtcc aacggctgag tcttcagcac
     2521 agaacgacaa ggcccccgtc gtatcggacg cgggccttgc gttcctagcg agtcgccggg
     2581 ctacttcttc tcgtcgccgg ccggtgccac cggcgccgac tgccccgcga cgaacgggta
     2641 cttcgagaag atgtgcgcga cggcctgatc gatggccgcc tgcttgttgg ccagcatctc
     2701 cttggtgacc acgccctcga tgccgccctg gaacacggct tggcgcttct cgcggtcgat
     2761 cacgtccatg acgagcgtgc cgacgttgta acggcgcgtc gtaacctcgg tgccgccgta
     2821 gccgtacggg gcgccgtacc agccgcggta gccccagcct ggaccataca tcggggccgg
     2881 cgtcgactcg at
//
LOCUS       DASPFR010000149        16106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_410660, whole genome shotgun sequence.
ACCESSION   DASPFR010000149 DASPFR010000000
VERSION     DASPFR010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16106
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410660"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /gene="ubiA"
                     /locus_tag="VD737_03710"
     CDS             complement(<1..698)
                     /gene="ubiA"
                     /locus_tag="VD737_03710"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR01474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HXW09694.1"
                     /translation="MTNLSDLDGEVSRANGMRGRLVEYARLMRLDRPIGIWLLLWPCL
                     WALWISAAGRPDERVFVIFLIGTFVMRSAGCVINDFADREFDPHVRRTASRPLARGAV
                     SPAEALAVFAVLGLIALALVIPLNRPTQTLALVGGTLAVTYPFLKRFFALPQAYLGLA
                     FSWSVPMAFAAQTGELPLVAWVLFAAGVLWTTAYDTIYAMVDREDDLVIGVRSSAILF
                     GRADRGIVAGLQVGA"
     gene            complement(711..2798)
                     /gene="recG"
                     /locus_tag="VD737_03715"
     CDS             complement(711..2798)
                     /gene="recG"
                     /locus_tag="VD737_03715"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008163.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HXW09695.1"
                     /translation="MSVAPLADRPVTALRGVGPALAESLARLGLQTVQDVLFHLPLRY
                     EDRTRVVPIGGLRHGDRAVIEGEIQLAEVAFRRRRMLLVRLADGTGFVTLRFFHFSAA
                     QQAQLARGQRLRCFGEVRVGPGGPEIVHPEYRRVSPGPGPGSGNETLTPIYPTTEGVQ
                     QGRLRALTDLALDLLARGAIADYVPPGVLDAFELPPLEAALRYVHRPPPDADVAELLA
                     GRHPAQRRLAFEELLAHQLSLRLLREATDHDRARPLPRSTDLAERCLAQLPFRLTGAQ
                     ARVWSEIERDLARPHPMMRLVQGDVGSGKTVVAALAALRAVEHDAQAAVMAPTELLAE
                     QHARNFHAWLDALDVRVALLTGRHSGRTRAALETDLADGSIQVAVGTHALFQEDIAFR
                     RLALVIVDEQHRFGVHQRLQLREKGRADDCYPHQLVMTATPIPRTLAMTAYADLDVSI
                     IDELPPGRTPIKTVVLPDSRRSEIVERIDAACRAGRQAYWVCPLIEESELVEYEAAEK
                     TAAQLAEALPGVRIGLVHGRMPGRRKDATMHSFKQGKLDLLVATTVIEVGVDVANASL
                     MVIENAERMGLAQLHQLRGRVGRGSVESTCVLLYQSPMSAIARERLAVLRQTNDGFEV
                     ARRDLELRGPGELLGTRQTGLMQLRVADLTRDADLLPDVQRAAALMLDGPRSNIAGLV
                     GRWVGAGERFRNA"
     gene            complement(2824..3204)
                     /locus_tag="VD737_03720"
     CDS             complement(2824..3204)
                     /locus_tag="VD737_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017700834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXW09696.1"
                     /translation="MPFTPVSTADAPGAIGTYSQAARAGSTVYLSGQIPLDPATMELV
                     SGDVEVHVRRVFDNLRAVARAAGGDLANAVKVNVYLTDLGHFQVVNKVMAEYFAQPYP
                     ARAAVGVAALPRGAIVEVDAVLVL"
     gene            complement(3321..3671)
                     /locus_tag="VD737_03725"
     CDS             complement(3321..3671)
                     /locus_tag="VD737_03725"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HXW09697.1"
                     /translation="MATAHEGTQHPIKVYLWIWFLLFVFSAFSYLVDYLHLQGPLRWT
                     LILLFMFLKAGFIVAIFMHMKWERLALKLVILVPPLCLLVLIALMAIEGDYTFLTRGT
                     AFAPDEPPAGHAAH"
     gene            complement(3683..4396)
                     /locus_tag="VD737_03730"
     CDS             complement(3683..4396)
                     /locus_tag="VD737_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III family protein"
                     /protein_id="HXW09698.1"
                     /translation="MSHVPHAVEVMTHPIEPAGTVPGLPGVVTDLAADKQAFPVPWGK
                     AMMWIFLLSDTFIFSCFLVSYMHVRMSTTVPWPNPSEVFALSFGGEPIPLILIAIMTF
                     VLISSSGTMAMAVNYGYRRNRARAASLMLITAAFGTTFVGMQAFEWTKLIVDEGIRPW
                     GNPLGAEQFGASFFMITGFHGMHVSAGVIYLVIVALKVWRGDYERAGGNYEIVEIAGL
                     YWHFVDLVWVFIFAFFYLW"
     gene            complement(4393..5088)
                     /locus_tag="VD737_03735"
     CDS             complement(4393..5088)
                     /locus_tag="VD737_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HXW09699.1"
                     /translation="MNLVVASVALALGILVWWMAARRLTSRPWEAQGTFDGRDGARIH
                     AEPPPTRVALWVFLAVVTSFFVLLMTAYVIRMSPHLVQNIDLRDWRPLAEPPVLWVNT
                     LLLAVGSVGMEIARRGLRHRQYERAARGLLAGGGFAIAFLVGQWLAWRQLQAAGYYAA
                     TNPANAFFYVLTGLHALHLLGGLVVWARATARVLRGSTSLETTRLSVDLCTVYWHYLL
                     AVWIVLLGLLVIT"
     gene            complement(5085..6848)
                     /locus_tag="VD737_03740"
     CDS             complement(5085..6848)
                     /locus_tag="VD737_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HXW09700.1"
                     /translation="MSYVAIADQHAHLHDPQTFITKYVWSQDHKVIAIQYMATAMAIG
                     VVAIALSILMRLQLGFPGTFNFINPENYYQFVSMHGMIMVIYLLTALFLGGFGNYLIP
                     LMCGARDMVFPYLNMVSYWVYLLAVLVLVASFFVPGGPTGAGWTLYPPQAILEGTPGA
                     DWGITLMLVSLIIFIVGFTMGGLNYVVTVLQARCRGMTLMRMPLSVWGIFTATILALL
                     AFPALLVSAIMMALDNHLHTSFFMPAIISLGDQTVHKGGSPLLFQHLFWFFGHPEVYI
                     VALPAFGIVSDLISTHARRHIFGYRMMVWAILAIGILSFVVWAHHMYVSGMNPYFGFF
                     FATTTLIIAIPTAIKVYNWVLTLWRGDVHLTLPMLFAIGFVFTFINGGLTGLFLGNVT
                     VDVPLSDTMFVVAHFHMVMGVSPVLVVCGAIYHWYPKMTGRMLDDRLGRLHFWVTLIG
                     TYAIFLPMHYLGILGVPRRYFALGETYFAPDSVHSMNAAISIAAIVVAMAQLIFIFNL
                     FWSLRNGKPAGPNPWRATTLEWQTPETPPRHGNWGPELPVVYRWAYEYGAPGNKHDFV
                     PQNEPPDDVTAVAVGHGRNVG"
     gene            complement(6883..8241)
                     /locus_tag="VD737_03745"
     CDS             complement(6883..8241)
                     /locus_tag="VD737_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXW09701.1"
                     /translation="MAIAVVLVLLVIGTVAFHVLNPWWFTPIASNWSTMDDTVNLTFW
                     VTGAVFVGVNLFMAYAIVRYRHRKGVERRAEYEPEYRRLEWWLIGLTAVGVAAMLAPG
                     LVVWAKFVTVPDDADVVEAVGQQWSWSFRLPGADGVLGAVDASLMAADNPFGMDPNDP
                     RGLDDVLIDGAELHLPLDRPVKLLLRSKDVLHDFTVPEFRVKMDLVPGMITYMWLTPT
                     RTGQFEILCEELCGTGHFAMRGRVVVDDEAAYQTWLAAQQTYAQRRERPPGDAVAGAT
                     QYAVCAACHGAQGEGNQALNAPKLAGLPAWYLSRQLHNFKHGVRGGAPGDAIASQMSA
                     IVQPLDDAMLANVVAHVASLPDVTAPTTVRGDAAAGARRYATCAYCHGARGQGSWSTN
                     APPLAGRSDWYLARQLQQFREGHRGRHPQDFHGSQMARLSNVVADGEPTADLLSYLNT
                     LR"
     gene            complement(8248..8817)
                     /locus_tag="VD737_03750"
     CDS             complement(8248..8817)
                     /locus_tag="VD737_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09702.1"
                     /translation="MKCLYYLAPTLDSTHGISDDLHSVGVDDFFVHVISRDEAGLRKQ
                     HIHSSNYLETLDVVRDGIIGAAVGFCVGLLGVWLIDRFDPFGVEVPMFVYIALVAVAT
                     LFGAWEGGLIGIGLENRKLARFHDDIEAGKFLVLIYALREQETAVAAMMRERHPEAEL
                     VGVDRHFVNPFSSPKREPVAPDERLLQKE"
     gene            complement(8883..9134)
                     /locus_tag="VD737_03755"
     CDS             complement(8883..9134)
                     /locus_tag="VD737_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09703.1"
                     /translation="MWASLITLVLLACLVLWLRAWQRGSRLALGLAIGAGLALLGGLF
                     LQSRPVFDHMPVWVPALPFAIIAITLFGFGALAWFWGED"
     gene            complement(9231..10043)
                     /locus_tag="VD737_03760"
     CDS             complement(9231..10043)
                     /locus_tag="VD737_03760"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HXW09704.1"
                     /translation="MRPPRRALRAAALAVGIFASAAMSFRAPAQSMDNAAEAAAIRAS
                     QAAIGTRTADLTLVDAAGAPLRLGDLRGRPVVVSFIFTNCYVVCSGLTLHLRDVVRIA
                     RETLGDGSFAVLTVGFDSEHDTPARLRAYARDRGITDADWHFVGGDPVTVRRLTDELG
                     FTWAPSPRGFDHVTQVTLLDGEGVVAAQLYGEAFEPPELVEPLKRLVLRQRVERSSVQ
                     GLIERVRLYCTVYDPAARRYRFDYSMLAAGLPPLFALAAVALGFAWLTRRRH"
     gene            complement(10058..12214)
                     /gene="spoT"
                     /locus_tag="VD737_03765"
     CDS             complement(10058..12214)
                     /gene="spoT"
                     /locus_tag="VD737_03765"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003195510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HXW09705.1"
                     /translation="MDVTSSLRSLLPYGRRSIGINQLISKLETYLPPDQVERVQEAYD
                     FAFHAHDGQRRRSGEPYITHPVAVADLLADLRLDAQTMIAAILHDVMEDTPNTKAEIT
                     DRFGREVAELVDGVSKLDQVQFRSRAEAQAESFRKMLLAMVRDIRVIMVKLADRTHNM
                     RTLGAMPPAKRRAIARETLEIYAPIANRLGMHAIKRELEDLGFRALYPRRYKVIESAV
                     KAAKGNQKQFVGRIASSLKESLDQSGIAARIEGREKDVYSIYEKMRRKKLSLNEVVDV
                     YGFRLVVDRPDTCYRTLGVVHSVFKPMPGRFKDYIAIPRVNGYQSLHTTLFGPNSTPI
                     EVQIRTEEMHRIAESGIAAHWQYKEGGGSDTGPQGDRAREWLQQLVEIQQGGNSEEFL
                     ESVKVDLFPDKVYVFTPKGEIRRLPRGATCVDFAYAVHTDVGNRCVAAKVDRRLVPLR
                     TPLRNGQTVEIITAKGATPNPAWVNFVVTAKARASIRQYLKSLKRSEAIELGKRLLNQ
                     ALEEFSLTLRKIPQESVTTVVAELGLRTQDELFEKIGLGERVAPFIARRLLPQDSAAI
                     AEGIAGPLAIAGTEGLVVAYARCCFPIPNDPILASLSSGRGVVIHREACGNLASFRKQ
                     PETWIPVTWQAASDRLFHVEIRADVTNRMGVLASVASAIAGTQTNIDRVAVVERDTDT
                     STLIFELLVHDRKQLASVMRAVRGMPEVLKVSRTLA"
     gene            complement(12231..12518)
                     /gene="rpoZ"
                     /locus_tag="VD737_03770"
     CDS             complement(12231..12518)
                     /gene="rpoZ"
                     /locus_tag="VD737_03770"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020293322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HXW09706.1"
                     /translation="MARITVEDCLQNVPNLFQLVLVAARRARKLANGSEATLEWENDK
                     PTVLALREIAAGHVGVEILEEPDLPPPPPPELMPEPEFMGEFRAPQIGLGD"
     gene            complement(12668..13309)
                     /gene="gmk"
                     /locus_tag="VD737_03775"
     CDS             complement(12668..13309)
                     /gene="gmk"
                     /locus_tag="VD737_03775"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HXW09707.1"
                     /translation="MSAASQPPRRGRLFVISAPSGAGKTSLVRALMERERTLRFSISY
                     TTRPQRPTETHGRDYFFVTGAEFEAMAGRGEFLEHARVFDHRYGTARGQVEASLGAGQ
                     DLILEIDWQGARQIREAMPECISIFILPPSRAELERRLRSRATDTDEVIRRRLRDAAS
                     DMTHWDEFDHVVVNDDFEAALETLLSIVRGFEAERAAARRPELRALVQELTQG"
     gene            complement(13309..14178)
                     /locus_tag="VD737_03780"
     CDS             complement(13309..14178)
                     /locus_tag="VD737_03780"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HXW09708.1"
                     /translation="MIRSMTGFARRERQGPWGTLTCEIRAVNHRYLELSLRMPEDLRA
                     IESDARLLVSAALRRGKVDVGLYLRGATAQAAAIEVNRALVEQLARTASDVASIAGAT
                     LAAVSPLDLLRWPGVIREAERDLQPTQAAAMELLRETVHELNESRGREGARIRDMLHG
                     RCESLQQGVALLRARLPDIAARIRTRVAERVTQLGTQVDPARLEQELVLLAYKMDFEE
                     ELDRLGSHVTETMQVLDAKEPAGRRLDFLMQELNREANTLSSKSQDAETTRAAVDIKV
                     LIEQMREQVQNVE"
     gene            14300..15046
                     /locus_tag="VD737_03785"
     CDS             14300..15046
                     /locus_tag="VD737_03785"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HXW09709.1"
                     /translation="MQIEHADLSLIGHREENQDRCAIADADGTVLLAVVDGMGGHADG
                     ARAAEVAIRTLLAEFWETSRPLFDPDGFLHLTVGRAHEAVVALGSGLPPEIRPRATCA
                     VCLVQGSSAYWAHVGDSRVYQLRGGRVLERTRDHSHVELLLRAGRITERQAQGHPMRN
                     YVECCIGGDPALPEMTLSGRRALQPNDVLMLCSDGLWSGLAEEQIASLSSDPHRDLRE
                     RLAELGELAVTNSAPYADNTTAAAIRWLKA"
     gene            15168..15914
                     /gene="rph"
                     /locus_tag="VD737_03790"
     CDS             15168..15914
                     /gene="rph"
                     /locus_tag="VD737_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HXW09710.1"
                     /translation="MRPSGRAPDQLRPVRITRGFTRHAEGSVLVEFGDTRVLVTATVE
                     DGVPGFLRGKGEGWVTAEYGMLPRSTHTRSAREAARGKQGGRTLEIQRLIGRSLRAVV
                     DLRALGERTVTLDCDVLQADGGTRTASITGAYVALADAMNALLRRRQLSSSPLHGQVA
                     AISVGIVRGEAVVDLDYVEDSGAETDMNVVMNNGGGFVEVQGTAEGHAFRRHELDRLL
                     DLAAGACDRLFALQLEALQAPLAAAAPTRA"
     gene            15911..>16106
                     /locus_tag="VD737_03795"
     CDS             15911..>16106
                     /locus_tag="VD737_03795"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HXW09711.1"
                     /translation="MTPRRLPPTVVLATSNAGKLREMREILAPWNVEVRSVDEYARQT
                     VEETGLTFVENALLKARHAAA"
BASE COUNT         2645 a         5448 c         5631 g         2382 t
ORIGIN      
        1 gcaccgacct gcaggccggc gacgatgccc cggtcggcgc gcccgaacag gatcgccgac
       61 gagcgcacgc cgatcaccag gtcgtcctcg cgatcgacca tggcgtagat cgtgtcgtaa
      121 gccgtcgtcc acagcacgcc cgccgcgaag agcacccacg cgaccagcgg cagctcgccg
      181 gtctgtgccg caaacgccat gggcaccgac cacgagaacg cgagcccgag gtacgcctgg
      241 ggcagcgcga agaagcgctt caagaacggg tacgtcaccg cgagcgtgcc gcccacgagc
      301 gcgagcgtct gcgtcgggcg attgagcggg atcaccagtg ccagcgcgat cagcccgagc
      361 accgcgaaca ccgcgagcgc ttcggccggc gacacggcgc cgcgcgcgag cggccgactg
      421 gccgtgcgac gcacgtgggg atcgaactcg cggtccgcga agtcgttgat cacgcagccc
      481 gccgagcgca tcacgaacgt accgatcagg aagatcacga acacgcgttc gtcgggccgc
      541 ccggccgccg agatccagag cgcccagagg cacggccaca gcagcagcca gatgccgatc
      601 ggacggtcga gccgcatcag tcgtgcgtac tcgaccaggc gcccacgcat gccgttcgcg
      661 cgggagactt cgccgtcgag gtcgcttaga ttcgtcattg caggctggac tcaggcgttg
      721 cggaagcgct cgccggcacc tacccagcgg ccgacgaggc ccgctatgtt ggaccgcggc
      781 ccgtccagca tcaatgccgc ggcccgctga acgtcgggca gcaggtctgc atctcgcgtc
      841 aggtcagcga cgcgcagttg catcagtccg gtctgacgcg tgccgagcag ttcgccgggg
      901 ccgcgcaact cgaggtcgcg cctggcgacc tcgaagccgt cgttggtctg ccgcagcacg
      961 gcgagtcgct cgcgggcgat cgccgacatc ggcgactggt acagcagcac gcaggtgctc
     1021 tcgacggagc cgcgtcccac ccgcccgcgc agctggtgca gctgtgcgag ccccatgcgc
     1081 tcggcgttct cgatcaccat caggctcgcg ttcgcgacgt cgacaccgac ctcgatcacg
     1141 gtggtcgcga ccagcaggtc gagcttgccc tgcttgaacg aatgcatcgt cgcgtccttg
     1201 cggcggccgg gcatgcgtcc gtgcacgagt ccgatgcgca cgccgggcag cgcctcggcg
     1261 agctgcgccg cggtcttctc ggccgcctcg tattccacga gctccgactc ctcgatcagc
     1321 gggcagaccc agtacgcctg gcgcccggcg cggcaggcgg cgtcgatgcg ctcgacgatc
     1381 tcgctgcgtc gcgaatcggg cagcaccacc gtcttgatcg gcgtccggcc cggcggcagc
     1441 tcgtcgatga tcgagacgtc gaggtcggca taggccgtca ttgcgagcgt gcgcgggatc
     1501 ggcgtggcgg tcatcacgag ctggtgcggg tagcaatcgt cggcacgacc tttctcccgc
     1561 agctgcagtc gctggtgcac gccgaagcga tgctgctcgt cgacgatcac gagcgccagg
     1621 cggcggaacg caatgtcctc ctggaacaac gcatgcgtgc cgacggcgac ctggatcgac
     1681 ccatcggcga ggtccgtttc gagcgccgcc cgtgtccggc cggagtggcg gccggtcagc
     1741 agcgcgacgc gtacgtcgag cgcgtcgagc cacgcgtgga agttgcgtgc gtgctgctcg
     1801 gcgagcagtt ccgtcggcgc catcacggcc gcctgcgcgt cgtgctcgac ggcacgcaat
     1861 gccgcgagcg cggccacgac ggtcttcccg gaacccacgt cgccctgcac gagccgcatc
     1921 atcgggtgcg gccgcgccag gtcgcgctcg atctcggacc agacgcgcgc ctgcgcgccg
     1981 gtcagccgaa acggcaactg cgcgaggcag cgctcggcga ggtccgtcga gcgcggcagc
     2041 ggccgcgcgc gatcgtgatc ggtcgcttcg cgcaacaggc gcaggctcag ctggtgcgcg
     2101 agcagctctt cgaacgcgag cctgcgctgg gccgggtgac gtccggccag cagctcggcg
     2161 acgtcggcgt cgggcggcgg ccgatgcacg tagcggagcg ccgcctcgag cggcgggagc
     2221 tcgaaggcgt cgagcacgcc gggcgggacg taatcggcga tggcaccccg cgcgagcagg
     2281 tcgagcgcga gatcggtcag cgcgcgcaaa cgcccctgct gcacgccctc cgtggtcggg
     2341 tagatgggcg tcagcgtctc gttgccggag cccggtccgg ggcccgggga tacgcgacgg
     2401 tactcgggat ggacgatctc gggcccaccg ggcccgaccc tcacttcgcc gaagcagcgc
     2461 agtcgctgtc cccgcgcgag ctgggcctgc tgcgcggccg agaagtggaa gaaacgcagc
     2521 gtgacgaagc cggtgccgtc cgccaggcgc accagcagca tccggcggcg gcggaacgcc
     2581 acttcggcga gctggatctc gccttcgatc acggcgcggt cgccgtgtcg cagccccccg
     2641 atcggcacga cgcgagtgcg atcctcgtag cgcagcggca ggtggaacag cacgtcctgc
     2701 acggtctgca gtccgaggcg cgcgagcgat tcggccagcg ccgggccgac gccgcgcaac
     2761 gccgtgaccg ggcgatcggc gagcggggcc acgctcatcg acgtcggggc ggtcgggacg
     2821 ggttcagagc accagcaccg cgtccacttc gacgatcgcg ccgcgcggca acgcggccac
     2881 gccgacggct gcccgcgccg ggtacggctg cgcgaagtat tccgccatga ccttgttgac
     2941 gacctggaag tggccgaggt cggtcaggta cacgttgacc ttgacggcgt tggcgaggtc
     3001 accgccggcc gcgcgcgcga cggcgcgcag gttgtcgaac acccgtcgca cgtggacttc
     3061 gacgtcgccg ctgacgagct ccatcgtcgc ggggtcgagc gggatctggc ccgacaggta
     3121 gaccgtgctg ccggcccggg cggcctgcga atacgtgccg atcgcgcccg gcgcgtcggc
     3181 ggtcgagacg ggggtgaagg gcacggaggc tccaaacgga agagtgtcta gtgttcagtg
     3241 tctagtgtct ggtaggaggg cgcaaccatg cgacacgggc acgccgatcc tactgaagac
     3301 tagacaccag acactgaaca ctagtgcgcc gcgtgtcctg ccggcggctc gtcgggggcg
     3361 aacgccgtgc cgcgagtcag gaacgtgtag tcgccctcga tcgccatcag cgcgatcagc
     3421 acgagcaggc agagcggcgg cacgaggatc acgagtttga gcgcgaggcg ttcccacttc
     3481 atgtgcatga agatggcgac gatgaagccg gccttcagga acatgaacag caggatcagc
     3541 gtccagcgca acggaccctg caggtgcagg tagtcgacca ggtacgagaa cgcgctgaac
     3601 acgaacagca ggaaccagat ccagaggtag accttgatcg gatgctgcgt gccctcgtgc
     3661 gccgttgcca tcgccctagc tcctaccaga gatagaaaaa cgcgaagatg aagacccaga
     3721 cgaggtcgac gaagtgccag tacaggcccg cgatctcgac gatctcgtag ttgccacccg
     3781 cgcgctcgta gtcgccacgc cagaccttga gcgccacgat caccaggtag atcacgcccg
     3841 ccgacacgtg catgccgtgg aaccccgtga tcatgaagaa cgaggcgccg aactgttccg
     3901 cgccgagcgg gttgccccag ggccggatgc cctcgtcgac gatcagcttg gtccactcga
     3961 acgcctgcat gccgacgaac gtcgtgccga acgccgccgt gatcagcatc agcgaggccg
     4021 cgcgcgcccg gttgcgccgg taaccgtagt tgacggccat cgccatcgtg ccgctgctcg
     4081 agatcagcac gaaggtcatg atcgcgatca ggatcagcgg gatcggctcg ccgccgaagc
     4141 tcagcgcgaa gacctcgctc gggttcggcc acggcacggt cgtggacatc cgcacgtgca
     4201 tgtacgagac caggaagcac gagaagatga acgtgtcgct gagcaggaag atccacatca
     4261 tcgccttgcc ccagggcacg gggaaggctt gcttgtccgc cgcgaggtcg gtgacgacgc
     4321 cgggcagccc cgggaccgtg cccgcgggct cgatcgggtg cgtcatgact tcgacggcgt
     4381 ggggcacgtg gctcatgtga tcaccagcag gccgaggagg acgatccaga ctgcgagcag
     4441 gtaatgccag tagacggtgc agaggtcgac gctgagccgg gtggtctcga gcgaggtgct
     4501 cccccgcaac acgcgcgcgg tcgcgcgggc ccagacgacg aggccgccga gcaggtgcag
     4561 cgcgtggagc cccgtcagca cgtagaagaa ggcgttggcc ggattcgttg ccgcgtagta
     4621 gcccgccgcc tgcaactggc gccacgcgag ccactggccg accaggaacg cgatcgcgaa
     4681 ccccccgccg gcgagcagcc cgcgggccgc gcgttcgtac tggcggtggc gcaggccccg
     4741 cctcgcgatc tccatgccga cgctgccgac cgcgagcagc agcgtgttga cccagaggac
     4801 cggcggttcc gccagcggcc gccagtcacg gaggtcgatg ttctgcacga ggtgagggct
     4861 catgcggatc acgtaggccg tcatcagcag cacgaagaac gacgtgacca cggcgaggaa
     4921 cacccagagc gcgacgcgcg tgggcggcgg ttcggcgtgg atgcgcgcgc cgtcgcggcc
     4981 gtcgaacgtg ccctgcgcct cccacggtcg gctcgtgagc cgccgcgccg ccatccacca
     5041 gacgaggatg ccgagggcca gcgcgacgga agcgacgacc aggttcatcc gacgttgcgc
     5101 ccgtggccga cggcgacggc ggtgacgtcg tccgggggct cgttctgcgg aacgaaatcg
     5161 tgcttgttgc ccggcgcgcc gtactcgtac gcccagcgat acacgaccgg cagctcgggg
     5221 ccccagttgc cgtgccgcgg cggtgtctcc ggggtctgcc actcgagcgt cgtcgcgcgc
     5281 cacgggttcg ggccggccgg cttgccgttc cgcagcgacc agaacaggtt gaagatgaag
     5341 atcagctgcg ccatcgcgac cacgatcgcc gcgatgctga tggcggcgtt catcgagtgc
     5401 acggaatccg gcgcgaagta ggtttcgccg agcgcgaagt agcggcgtgg cacgccgagg
     5461 atgccgaggt agtgcatcgg caggaagatc gcgtacgtgc cgatcagcgt gacccagaag
     5521 tgcagccggc cgagccggtc gtcgagcatc cggcccgtca ttttcgggta ccagtggtag
     5581 atcgccccgc acacgaccag caccggcgag acgcccatga ccatgtggaa gtgcgcgacc
     5641 acgaacatcg tgtccgagag cggcacgtcg accgtgacgt tgcccaggaa cagcccggta
     5701 agcccgccgt tgatgaacgt gaagacgaag ccgatcgcga acagcatcgg cagcgtcagg
     5761 tgcacgtcgc cgcgccagag cgtgagcacc cagttgtaga ccttgatcgc ggtcgggatc
     5821 gcgatgatca gcgttgtggt cgcgaagaag aacccgaagt acgggttcat gccactcacg
     5881 tacatgtggt gcgcccagac cacgaaactc aggatcccga tcgccaggat cgcccagacc
     5941 atcatccggt agccgaagat gtgccggcgc gcgtgcgtgc tgatcaggtc ggagacgatg
     6001 ccgaacgccg gcagcgcgac gatgtagacc tcggggtggc cgaagaacca gaacaggtgc
     6061 tggaacagca gcggactgcc gcccttgtgc acggtctggt cgccgagcga gatgatggcc
     6121 ggcatgaaga agctcgtgtg caggtggttg tcgagcgcca tcatgatggc gctcaccagc
     6181 agcgccggga acgcgagcag cgccaggatc gtcgccgtga agatgcccca gaccgacagc
     6241 ggcatccgca tcagcgtcat gccgcggcaa cgcgcctgca gcaccgtcac gacgtagttg
     6301 aggccgccca tcgtgaagcc gacgatgaag atgatcagcg agaccagcat cagcgtgatg
     6361 ccccagtccg cgccgggcgt gccttccagg atcgcctgcg gcgggtagag cgtccagccg
     6421 gcgcccgtgg ggccgcccgg cacgaagaag ctcgcgacca gcaccagcac cgcgagcagg
     6481 tagacccagt agctgaccat gttcaggtac gggaacacca tgtcgcgcgc gccgcacatc
     6541 agcgggatca ggtagttgcc gaagccgccg aggaacagcg ccgtcagcag gtagatcacc
     6601 atgatcatgc cgtgcatgct gacgaactgg taatagttct ccgggttgat gaagttgaag
     6661 gtgccgggga acccgagctg cagccgcatc aggatcgaca gcgcgatcgc gaccacgccg
     6721 atcgccatcg ccgtggccat gtactggatc gcgatgacct tgtggtcctg gctccagacg
     6781 tacttggtga tgaaggtctg cgggtcgtgc aggtgcgcgt gctggtcggc gatcgcgacg
     6841 tatgacatct gtacctctcg atgaccgccg tggtcggggc cttcagcgca gcgtgttcag
     6901 gtaggagagc aggtccgcgg tcggctcccc gtcggcgacg acgttgctga gtcgcgccat
     6961 ctgggaaccg tggaagtcct gcggatggcg tccacggtgg ccttcgcgga actgctgcag
     7021 ctgccgggcc aggtaccagt cgctgcggcc ggcgagcggc ggcgcgttgg tggaccagga
     7081 accctggccg cgggcgccgt ggcagtacgc acaggtggcg tagcggcgcg cgccggctgc
     7141 ggcgtcgccg cgcaccgtgg tcggggcggt cacgtccggc agcgacgcga cgtgcgcgac
     7201 cacgttggcg agcatcgcgt cgtcgagcgg ctggacgatc gcggacatct ggctcgcgat
     7261 cgcgtcgccc ggcgcgcctc cccggacgcc gtgcttgaag ttgtgcagct ggcgcgacag
     7321 gtaccaggcg ggcaggcctg cgagtttcgg tgcgttcagc gcctggttgc cttcgccctg
     7381 cgcgccgtga caggcggcac agaccgcata ctgcgtggca cccgcgaccg cgtcgcccgg
     7441 gggccgctca cgccgctgcg cgtaggtctg ctgcgccgcg agccaggtct ggtaggcggc
     7501 ctcgtcgtcg acgacgacgc ggccgcgcat ggcgaagtgg cccgtgccgc aaagttcttc
     7561 gcagaggatc tcgaactgcc cggtgcgggt gggcgtgagc cacatgtagg tgatcatccc
     7621 cggcaccagg tccatcttga cccggaactc cggcaccgtg aaatcgtgca gcacgtcctt
     7681 cgagcgcagc agcagcttga ccggccggtc gagcggcagg tgcagttccg cgccgtcgat
     7741 cagcacgtcg tcgaggccgc gcgggtcgtt gggatccatg ccgaacgggt tgtccgcggc
     7801 catcagcgac gcgtcgaccg cgccgagcac gccgtccgcg cccggcaacc ggaagctcca
     7861 ggaccactgc tggcccacgg cttcgacgac gtccgcgtcg tcgggcaccg tcacgaactt
     7921 ggcccagacc acgagccccg gcgcgagcat ggcggcgacg cccaccgccg tgaggccgat
     7981 cagccaccac tccaggcgcc ggtactcggg ctcgtattcg gcgcgccgct cgacgccctt
     8041 gcggtgccgg tagcggacga tcgcgtaagc catgaacagg ttgacgccga cgaacacggc
     8101 gccggtcacc cagaacgtca ggttcacggt gtcgtccatc gtgctccagt tcgacgcgat
     8161 cggcgtgaac caccacgggt tgagcacgtg aaaagccacc gtcccgatca ccagcaggac
     8221 gagcacgaca gcgatcgcca tcgcggctta ctccttctgc aggaggcgtt cgtccggcgc
     8281 gacgggctcg cgcttgggcg agctgaacgg attgacgaag tggcggtcga ccccgaccag
     8341 ctccgcttcg ggatgccgtt cgcgcatcat cgctgcgacc gccgtttcct gctccctgag
     8401 cgcgtagatc aggaccagga acttgccggc ctcgatgtcg tcgtggaagc gcgcgagctt
     8461 gcggttctcg aggccgatgc cgatcaggcc gccttcccac gccccgaaca gcgtcgcgac
     8521 cgcgaccagt gcaatgtaga cgaacatggg gacctcgacg ccgaacgggt cgaagcggtc
     8581 gatcagccac acgccgagca ggccgacgca gaagccgacc gccgcgccga tgatgccgtc
     8641 gcggacgacg tcgagcgtct cgaggtagtt gctcgagtgg atgtgctgct tgcggaggcc
     8701 agcctcgtca cgcgagatca cgtgcacgaa gaagtcgtcg acgccgaccg agtgcaggtc
     8761 gtccgagatg ccgtgggtgc tgtcgagcgt cggcgccaga taatagaggc acttcatact
     8821 ttcccccgcg atgagacctt ggaccttccg ggcgcgaatg tcctcgtcgt gaacagagcc
     8881 ggctagtcct cgccccagaa ccaggcgagc gcgccgaagc cgaacaacgt gatggcgatg
     8941 atggcgaacg gcagggcggg cacccacacc ggcatgtgat cgaagaccgg gcgcgactgc
     9001 aggaacaggc cgccgagcag ggcgaggccc gcgccgatcg cgaggccgag cgcaagcctc
     9061 gagccgcgct gccaggctcg cagccacagg accaagcagg ccagcagcac gagcgtgatc
     9121 agggatgccc acatgcttcg aatctctgac agaaactttg cgccgtcaag gccaatgcga
     9181 atacgtcgcg ttttgcgttg cagcgagagc aacgcgcgac cgcgccctga tcagtgacgg
     9241 cggcgcgtca gccaggcgaa gcccagcgcc accgcggcca gcgcgaagag cggcggcagg
     9301 ccggccgcca gcatcgagta gtcgaagcga taacgccgcg ccgccgggtc gtagacggtg
     9361 cagtacaggc gcacgcgctc gatgagcccc tgcacggagg agcgctcgac gcgctgccgg
     9421 agcaccaggc gcttcagcgg ctccaccagt tccggcggct cgaacgcctc gccatacagc
     9481 tgcgcagcga ccacgccctc gccgtcgagc agcgtgacct gcgtgacgtg gtcgaagccg
     9541 cgcggcgagg gcgcccacgt gaagccgagc tcgtccgtca ggcgtcgcac ggtgacggga
     9601 tcgccgccca cgaagtgcca gtcggcgtcg gtgatgccgc ggtcgcgcgc atacgcccgc
     9661 aggcgcgccg gcgtgtcgtg ctcgctgtcg aagccgacgg tcaacacggc gaagctgccg
     9721 tcgcccagcg tctcgcgcgc gatccgcacg acgtcgcgca ggtgcagcgt gagccccgag
     9781 cagacgacgt agcagttggt gaagatgaag ctgaccacga cggggcggcc gcgcaggtca
     9841 ccgagtcgta acggagcacc ggccgcgtcg accagcgtca ggtccgcagt ccgcgtaccg
     9901 atcgcggcct ggctcgcacg gatcgccgcc gcctcggcag cgttgtccat ggactgcgcc
     9961 ggggcgcgga agctcatcgc cgccgaagcg aaaatcccga ccgcgagcgc cgccgcgcgc
    10021 aatgcgcggc gtggcggccg catgtctggc tcgcgcgtca cgcgagcgtt cgcgacacct
    10081 tgagcacttc cggcatcccg cgcaccgcgc gcatcacgct cgcgagctgt ttccggtcgt
    10141 gcacgagcag ctcgaagatc aacgtggagg tgtcggtgtc gcgctcgacc acggcgacgc
    10201 gatcgatgtt ggtctgcgtg ccggcgatcg ccgaggcgac cgaggccagc acgcccatcc
    10261 ggttggtgac gtccgcccgg atctcgacgt ggaacaggcg atcggaagcc gcctgccacg
    10321 tcacggggat ccacgtctcg ggctgcttgc ggaacgaggc gagattgccg cacgcctcgc
    10381 ggtggatcac gactccgcga ccgctcgaca ggctcgcgag gatgggatcg ttcgggatcg
    10441 ggaagcagca gcgcgcatac gcgaccacga ggccttcggt cccggcgatc gcgagcggac
    10501 cggcgatgcc ttcggcgatc gcggcggaat cctgtggcag caggcgacgg gcgatgaacg
    10561 gcgcgacgcg ttcgccgagg ccgatcttct cgaacagctc gtcctgcgtg cgcagcccga
    10621 gctcggccac cacggtggtg accgactcct gcgggatctt gcgcaacgtg agcgaaaatt
    10681 cctcgagagc ctggttgagc agccgcttgc cgagttcgat ggcttcgctg cgcttcaaac
    10741 tcttgaggta ttggcggatc gacgcgcggg ccttggccgt cacgacgaaa ttgacccagg
    10801 ccgggttcgg ggtggccccc ttcgcggtga tgatctcgac ggtctggccg ttgcgcagcg
    10861 gggtgcgcag cggcaccaga cgacggtcga ccttggccgc gacgcagcgg ttgccgacgt
    10921 ccgtgtgcac ggcgtaggcg aagtcgacgc acgtcgcgcc gcgcggcagc cggcggatct
    10981 cgcctttcgg cgtgaagacg tacaccttgt cggggaagag gtcgaccttg acgctctcga
    11041 ggaactcctc cgagttgccg ccctgctgga tctcgacgag ctgctgcagc cactcgcgcg
    11101 cgcgatcgcc ctgcggtccg gtgtcggagc cgccgccttc cttgtactgc cagtgcgccg
    11161 cgatgccgga ctccgcgatc cggtgcatct cctcggtgcg gatctgcacc tcgatcgggg
    11221 tgctgttcgg gccgaacagc gtcgtgtgca acgactggta gccgttcacg cgcgggatcg
    11281 cgatgtagtc cttgaaccgg cccggcatcg gcttgaagac cgaatgcacg acgccgagcg
    11341 tgcggtagca ggtgtcgggg cggtcgacga ccaggcggaa gccgtagacg tcgaccacct
    11401 cgttgagcga cagcttcttg cgccgcatct tctcgtagat gctgtagacg tccttctccc
    11461 ggccttcgat gcgcgcggcg atccccgact ggtcgagcga ctccttgagc gaggaggcga
    11521 tgcggccgac gaactgcttc tggttgccct tggccgcctt caccgcgctc tcgatgacct
    11581 tgtagcgacg cgggtagagc gcacggaacc ccaggtcctc gagctcgcgc ttgatcgcat
    11641 gcatgccgag ccggttggcg atcggtgcgt agatctcgag cgtctcgcgc gcgatcgcgc
    11701 gccgcttcgc cggcggcatc gccccgagcg tgcgcatgtt gtgggtgcgg tcggcgagct
    11761 tgaccatgat cacgcggatg tcgcgcacca tggcgagcag catcttgcgg aagctctcgg
    11821 cctgcgcctc ggcgcggctg cggaactgca cctggtcgag cttgctgacg ccatcgacca
    11881 gttcggccac ctcacgcccg aagcggtcgg tgatctcggc cttggtgttg ggggtgtcct
    11941 ccatgacgtc gtggaggatc gcggcgatca tcgtctgcgc gtccagccgc aggtcagcga
    12001 gcaggtcggc caccgcgacc gggtgcgtga tgtacggctc gcccgagcgg cggcgctgcc
    12061 cgtcgtgcgc gtgaaacgcg aagtcgtacg cctcctgcac ccgctcgacc tggtcgggcg
    12121 gcaggtaggt ttcgagtttc gagatgagct ggttgatccc tatggaccgt cttccgtagg
    12181 gcagcaggct gcgcagggaa gacgtgacgt ccatgggacc gggggcgggg tcagtctccc
    12241 agaccgatct gcggcgcgcg gaactcgccc atgaactcgg gctccggcat cagctccggc
    12301 ggcgggggcg gcggcaggtc cggctcctcg aggatctcga ccccgacgtg gccggccgcg
    12361 atctcccgca gcgcgagcac cgtcggcttg tcgttctccc actcgagcgt cgcttcggac
    12421 ccgttcgcga gcttgcgcgc gcggcgcgcc gccacgagca ccagctggaa caggttcggg
    12481 acgttctgca ggcagtcttc gacggtgatc cgggccatgg gtctgggtat tgcaggggga
    12541 aaggggcgaa agcataacaa ggagaggcgg caggggatag gggacgggcg ccgcgcagga
    12601 ggcacccgcc ccgggcacgc gacgcgcctc gttacgcgcc tggggcctgc cgcgatcgcc
    12661 ggacttccta gccctgcgtg agttcctgca ccagcgcccg aagttccggc cgtcgtgccg
    12721 cggcgcgttc cgcttcgaag cctcggacga tgctcagcag ggtttccagc gccgcctcga
    12781 agtcgtcgtt gacgacgacg tgatcgaact cgtcccagtg ggtcatgtcg ctcgccgcgt
    12841 cgcgcaggcg ccggcggatg acctcgtcgg tgtcggtggc gcggctgcgc agccgtcgct
    12901 cgagttcggc gcgcgacggc ggcaggatga agatcgaaat gcactccggc atcgcctcgc
    12961 ggatctgccg cgccccctgc cagtcgatct cgaggatcag gtcctggccg gcgccgagcg
    13021 atgcttcgac ctggccgcgt gcggtgccgt accgatggtc gaacacccgc gcgtgctcga
    13081 ggaactcgcc gcgtccggcc atcgcctcga actcggcacc cgtgacgaag aagtaatcgc
    13141 gaccgtgcgt ctcggtcggt cgttgcggcc gcgtcgtgta cgagatcgag aaacgcagcg
    13201 tccgctcccg ctccatcaac gcgcggacca gcgaggtctt gcctgcgccc gagggcgcgg
    13261 aaatcacgaa cagacggccg cggcgcggcg gctgggacgc ggcgctcatc actcgacgtt
    13321 ctggacctgt tcccgcatct gctcgatcag caccttgatg tcgaccgccg cccgcgtcgt
    13381 ctcggcatcc tgcgacttcg acgacagcgt gttggcctcg cggttgagct cctgcatcag
    13441 gaagtcgaga cgacggccgg cgggctcctt cgcgtccagg acctgcatcg tctcggtcac
    13501 gtggctgccg aggcggtcga gctcctcctc gaagtccatc ttgtaggcga gcagcacgag
    13561 ctcctgctcg agccgggcgg gatcgacctg cgtgccgagc tgcgtcacgc gctccgcgac
    13621 tcgcgtccgg atgcgtgccg cgatgtcggg caggcgtgcc cgcagcagcg ccacgccctg
    13681 ctgcagcgat tcgcagcgac catgcagcat gtcgcggatc cgcgcgcctt cgcggccgcg
    13741 ggattcattg agttcgtgca cggtctcacg cagcagttcc atggccgccg cctgcgtcgg
    13801 ctgcaggtcc ctttccgctt cgcggatcac gcccggccac cgcagcaggt cgagcgggct
    13861 caccgcggcg agcgtggcgc cggcaatcga ggctacgtcg gacgcggtgc gcgcgagctg
    13921 ctcgaccagc gcgcgattga cctcgatggc ggccgcctgc gccgtcgccc cgcgcagata
    13981 caggcccacg tcgaccttgc cgcgacgcag ggccgcggag acgaggaggc gcgcgtcgct
    14041 ctcgatcgca cgcaggtcct cgggcatccg cagcgacaac tccaggtaac ggtggttgac
    14101 cgcgcgaatc tcgcacgtca acgtgcccca ggggccttgg cgctcgcgtc gcgcgaagcc
    14161 ggtcatgctg cggatcatcg gcagtgggtt cggctgactg gaaagaaaac cgacggcggc
    14221 acgtcgctgt atgtgcgctg cgtcaagtgc tataaaccat tgataagtct acacgacatc
    14281 gggcgttccg gagcgtccct tgcagatcga gcacgcggac ctcagcctca tcgggcaccg
    14341 cgaagaaaac caggatcgct gcgcgatcgc cgatgccgac gggacggtgc tgcttgcagt
    14401 cgtcgacggc atgggcggtc acgccgacgg cgcacgtgcg gcggaagtgg cgatccgcac
    14461 gctgctcgcc gaattctggg agacgagtcg accgctgttc gatcccgacg gcttcctgca
    14521 cctgacggtg ggccgcgctc acgaagcggt cgtcgcgctc ggcagcggcc tgccgccgga
    14581 gatccgtccg cgtgccacgt gcgcggtgtg cctcgtgcag ggcagcagcg cgtactgggc
    14641 gcacgtcggc gacagccgcg tctaccagct gcggggcggc agggtgctcg agcgcacccg
    14701 cgatcattcg cacgtcgagc tgctgctgcg tgccggacgg atcacggagc ggcaggcgca
    14761 gggccatccg atgcgcaatt acgtcgagtg ctgcatcggc ggtgatccgg cgctgccgga
    14821 gatgacgctg agcgggcgcc gtgcactgca gcccaacgac gtgctgatgc tctgctccga
    14881 tggcttgtgg tcggggctcg ccgaagaaca gatcgcctcg ctcagcagcg acccgcaccg
    14941 cgatctgcgc gaacgcctcg ccgaactcgg cgaactcgcc gtgacgaact cggcccccta
    15001 cgcggacaat acgaccgccg ccgcgatccg ctggctgaaa gcctgaggag agtggctcgt
    15061 ggtcagtggc tagtggctag tcaggaggcg aacgcttgaa acgcgacgtc acttcctcac
    15121 gagccactgg ccactagcca ctgaacactt ccaccggaga ttcccccttg agaccttctg
    15181 gccgcgcgcc cgatcaactg cgtcccgtac ggattacgcg cgggtttacc cggcatgccg
    15241 aaggttctgt gctggtcgag ttcggcgaca cgcgcgtgct ggtcacggcg acggtcgagg
    15301 acggggttcc cggattcctg cgcggcaagg gcgaaggctg ggtgacggcc gagtacggca
    15361 tgctcccccg ttccacccac acccgcagcg cgcgcgaggc ggcacgaggc aagcagggcg
    15421 gccgcacgct tgagatccag cggctgatcg gacgctcgct gcgtgccgtg gtcgacctgc
    15481 gcgcgctcgg cgagcgcacg gtcacgctcg actgcgacgt gctgcaggcg gacggcggga
    15541 cgcgcacggc ctcgatcacg ggcgcatacg tggcgctcgc cgacgcaatg aacgcgctgt
    15601 tgcgtcgccg gcagctctcg tccagcccgc tgcacggaca ggtcgccgcc atctcggtcg
    15661 gcatcgtccg cggggaggcg gtcgtggacc tcgactacgt cgaggattcc ggcgccgaga
    15721 ccgacatgaa cgtcgtcatg aacaacggcg gaggcttcgt cgaggtgcag ggcaccgctg
    15781 aaggtcacgc gtttcgccgc cacgagctcg atcgcctgct cgacctggcc gcgggcgcct
    15841 gcgaccggct cttcgcgctg cagctcgagg cgttgcaggc gccgctggcg gcagcggcgc
    15901 ccacccgggc atgacaccgc gacgactgcc gccgacggtc gtgctcgcga ccagcaatgc
    15961 gggcaagctg cgcgaaatgc gggagatcct ggcgccgtgg aacgtcgagg tccgctcggt
    16021 cgacgagtac gcgcggcaga cggtcgagga gacggggctc acgttcgtcg agaatgcgct
    16081 gctcaaggcc cggcacgccg cggccg
//
LOCUS       DASPFR010000150         6673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1632565, whole genome shotgun sequence.
ACCESSION   DASPFR010000150 DASPFR010000000
VERSION     DASPFR010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632565"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..624
                     /locus_tag="VD737_03800"
     CDS             187..624
                     /locus_tag="VD737_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXW09712.1"
                     /translation="MHKDSYAGFRAAWLGVVATLLAVTAGAGEPSGNQAAPAAVMTQE
                     QLVEHLAKHPQHLFVLDVRSPQEYAEGHVPGAVNVPHDQLASRLAEVPKDKDVVLYCR
                     SGRRSALAADVLAANGYTRLSHLEGDMQAWVARGRPVEQPKAP"
     gene            complement(809..1936)
                     /locus_tag="VD737_03805"
     CDS             complement(809..1936)
                     /locus_tag="VD737_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HXW09713.1"
                     /translation="MKLETLAIHAGRHVDTATGAVREPLHLSTTFERDADGGYPRGYY
                     YTRSGNPNRTSLEQAVAALERGHEAVAFGSGSAATLAAFQLATPGGRIVCGRDCYHGT
                     AAQLREIVPAWGVDVEFVDTTDLAAVEAALDRAPTSLLWFETPTNPMLQVSDIEALAG
                     LAHARGALVGCDNTFASPVLQRPLALGADLVMHSTTKYLGGHSDVLGGIAVVGGPGRV
                     LEGLRHMQGTGGGVPSPFDCWLVLRSLATLPLRVRQQAANALAVAHFLHADRRRVQRV
                     HYAGLPDHPGHSIALRQMEGGFGAVLSMEVEGGKERALAVAARTRLFTRATSLGGVES
                     LIEHRASVEGPRTQTPPGLLRLSIGLEHADDLLADLDQALG"
     gene            complement(1933..3771)
                     /locus_tag="VD737_03810"
     CDS             complement(1933..3771)
                     /locus_tag="VD737_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HXW09714.1"
                     /translation="MNAVAAPAGTLDGLRLNFALQAGILRLLSKQEHLNKINVFPVPD
                     GDTGTNLALTARAVLATLRRLPDPHAGKTLTRVADAALDGARGNSGAILAQFFLGLGD
                     RLGHLTRLDPDDFVEGVAGGADYARESVTDPREGTILTVLTDFAREVQRARRDGLADF
                     RSLFRRGVVAAQASLEQTRFQLEALRRANVVDAGAQGFVELVAGLSDFLESGAETAPP
                     DGADAVVVLDASSEVAAGTEEDLAWRYCTECVVTGGHVDRRHLREALSTLGGSLVVAG
                     LQHKVRVHVHVDDPAEVFRVAARFGVVSGQKADDMQRQQHAAHRAERHVAVVVDSAAD
                     LPEDEMDRLGIHMVPVRVHFGDRSYLDKLGLSPEEFYAEIARGGEPPKTSQPPPGDFR
                     RQFEFLASHYEAVVSINLTSRASGTHAAAETAAARTSSHGHVTVIDSRNASVGQGLLA
                     MYAAECAAAGQRTDEVVAATRAMIPRTRTFGMVGSLEFAVRGGRVSPWVKRIADALRL
                     VPVLHADHEGRVTLGGVLFGRANATVKFARFVRRRMRRDRTYRVLVGHANCETEGQWL
                     LEMLRSPGVGYARLVPLGTALGAHGGPGMLVAAFQPYEAPAEAQPP"
     gene            complement(3788..4588)
                     /locus_tag="VD737_03815"
     CDS             complement(3788..4588)
                     /locus_tag="VD737_03815"
                     /inference="COORDINATES: protein motif:HMM:NF027851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HXW09715.1"
                     /translation="MSFILDALRKSEHARQKQTGPGLAEVPVVATKPKSNVWATAAIA
                     LLVVNLVAVGVLLLRRANDETPPAAAASAPVTTAPARPVADAAAPPATPSAAEPTLSS
                     EPPPPVTTPVARDSAPRPAAGGRNPLAEEVGGAVDPSLVAAASAPPPGPPAVTRQAAP
                     TRRGGTVVYAPIPEAEDLSYAPPPAAPAPAADNLPTADEVSARGGLPELHLDLHVYAN
                     TPQQRFIFVNSRKYREGEALQEGPIVEQITATGAVLSYRGARFKLTND"
     gene            complement(4590..6254)
                     /locus_tag="VD737_03820"
     CDS             complement(4590..6254)
                     /locus_tag="VD737_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXW09716.1"
                     /translation="MYTSFFGLQEKPFAITPDPRYLYLSERHAEALAHLLYGINEAGG
                     FIQLTGEVGTGKTTVIRSLLEQLPGHAEVALILNPRVTPAEFLLTICEELHLQVPEQG
                     RGSTKALMDLLGHYLLEAHARGRRVVLIVDEAQNLSPQTLEQVRLLTNLETATTKLLQ
                     IILIGQEELRTLLEQPDLRQLAQRITGRYHLSPLSRDETAGYVKHRMRVAGATAEAFT
                     PGALKEVHRLSGGIPRVINVVCDRALLGAFTREDHRVGPAYVRLAAAEVYGRPVPAPW
                     LRWATIGAVTAALALVGVGAWTLFATRAGAPTAASDATAAAPAAPVDPAPAAADATVD
                     AAQAPPAPALDELLVQNANDTTTEAALSKLFARWNLAYAANRGRGCDQATAQGLECLF
                     QKGSWAQLRTLNRPAILTLTDDLGSTHQVLLVGLDDEAATIDLGGSALAVPIAAISRY
                     WFGDFLLLWRPPLAVVKSLVPGMRGAEVKWLRENLRVAQGLPQAAPGNDVYDEELTRL
                     VQDFQRQHRLNADGVAGVQTQIVLDTVLNPTGSPTLGDDRAASVGG"
     gene            complement(6292..>6673)
                     /locus_tag="VD737_03825"
     CDS             complement(6292..>6673)
                     /locus_tag="VD737_03825"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HXW09717.1"
                     /translation="LGSTLSAVLLDTNIALADGVLAFALLIGLQFVVTWTSVRTRWVR
                     RLVTGEPQLLLYRGEFLPDALRSARVAKEEVRAAVRAAGVSCLEEAEAVVLETDGSFS
                     VVRGDAGGGETSLEGVQRYPSTSP"
BASE COUNT          956 a         2361 c         2396 g          960 t
ORIGIN      
        1 atggtcgagg tgtcgcggct gatcgacccc gacatgctgg tggagatcga ggccgacgcg
       61 gtcatcgtcg atgcgggtta gcgtacgatt gacgtcgcta ccgacgacgc cggtcgccgc
      121 gagcggcgtc ttcgtcgagc tcacggtcgt gggcgattca ccgcggctgc cgcgaaggag
      181 gcattcatgc acaaggattc gtacgctgga ttccgggccg cgtggctcgg cgtggtggcg
      241 acactgttgg ccgtgaccgc cggagccggc gaaccgtccg gcaaccaggc cgcgcccgcg
      301 gcggtcatga cgcaggaaca gctggtcgaa catctcgcga agcacccgca gcacctgttc
      361 gtgctcgacg tccgctcgcc gcaggaatac gccgaaggcc acgtgcccgg cgcggtcaac
      421 gtgccgcacg atcagctggc ttcccgcctg gccgaggtgc cgaaggacaa ggacgtcgtg
      481 ctgtactgcc gcagtggccg tcgctcggcc ctggcggcgg acgtgctggc ggcgaacggc
      541 tacacgcgcc tgtcgcacct cgagggcgac atgcaggcgt gggtcgcccg cggccggccc
      601 gtcgagcagc ccaaggcccc gtaattgggg acactccgga atttccctga attggggaca
      661 ctccggattt cacgaaaaca ccgggtaaaa cggccctccg ccgtacgccg gaaactccgg
      721 tcaaccgctg ctgcatcgaa aaatgcagca cgaaaaacag gagtgtcccc aattagccgt
      781 taattcggag tgtccccatt tatcccattt atccgagggc ctggtcgagg tcggcgagca
      841 ggtcgtccgc gtgctcgagc ccgatcgaca ggcggagcag gcccggcggc gtctgggtgc
      901 gcgggccttc cacggacgcg cgatgctcga tcaggctttc gacgccgccc aggctggtcg
      961 cccgcgtgaa cagccgggtc cgcgccgcca cggcgagcgc gcgttcctta ccgccctcga
     1021 cttccatcga cagcaccgcg ccgaagccac cctccatctg ccgcagcgcg atcgagtgac
     1081 cggggtggtc cggcaggccg gcgtagtgca cgcgctgcac gcgccgacgg tccgcgtgca
     1141 ggaaatgcgc gaccgcgagt gcgttcgcgg cctgctggcg cacgcgcagc ggcagcgtgg
     1201 cgaggctgcg cagcacgagc cagcagtcga acggcgatgg cacgccgccg ccggtgccct
     1261 gcatgtggcg caggccctcc agcacgcggc cgggcccgcc cacgaccgcg atgccgccga
     1321 gcacgtcgct gtggccaccg aggtacttgg tggtgctgtg catcaccagg tcggcaccga
     1381 gcgccagcgg ccgctgcagc accgggctcg cgaaggtgtt gtcgcagccc accagcgcgc
     1441 cgcgcgcgtg ggcgaggccg gcgagcgcct cgatgtcgga cacctgcagc atcgggttgg
     1501 tcggcgtctc gaaccagagc aggctcgtcg gggcgcggtc gagcgcggcc tcgacggccg
     1561 cgaggtccgt cgtgtcgacg aactcgacgt cgacgcccca cgccggaacg atctcgcgca
     1621 gctgcgcggc ggtcccgtga taacagtcac gaccgcagac gatcctcccg cccggcgtgg
     1681 ccagctggaa cgcggccagc gtggccgcgg agcccgaacc gaatgccacg gcttcgtgac
     1741 cgcgctcgag cgcggcgacg gcctgctcga gcgacgtccg gttgggattg cccgatcgcg
     1801 tgtagtagta gccacgcgga tacccgccgt cggcgtcgcg ctcgaacgtc gtcgacaggt
     1861 gcaggggttc gcgcacggcg ccggtcgcgg tgtcgacgtg gcggccggca tggatcgcga
     1921 gtgtctcgag tttcatgggg gctgcgcctc ggccggcgcc tcgtacggct ggaacgccgc
     1981 gacgagcata cccggtccgc cgtgcgcgcc gagtgccgtg ccgagcggaa ccagccgcgc
     2041 gtagccgacc ccgggcgagc gcagcatctc gagcagccac tggccttcgg tctcgcaatt
     2101 ggcgtggccg acgagcacac ggtaggtgcg atccctgcgc atgcggcggc gcacgaagcg
     2161 cgcgaacttc acggtcgcgt tggcgcgccc gaacaggacg ccgccgagcg tgacgcggcc
     2221 ttcgtggtcg gcgtgcagca ccggcaccag gcgcagcgcg tcggcgatgc gcttgaccca
     2281 cggcgagacg cgtccgccgc gcacggcgaa ttccagcgag ccgaccatgc cgaacgtgcg
     2341 cgttcgcggg atcatcgcgc gcgtcgccgc gaccacttcg tccgtgcgct ggccggcggc
     2401 cgcgcactcg gcggcgtaca tcgcgagcag tccctggccc accgacgcgt tgcggctgtc
     2461 gatcacggtg acgtggccgt gcgagctcgt gcgggccgcc gcggtctccg ccgcggcgtg
     2521 agtgccgctg gcgcggctgg tcaggttgat cgagacgacg gcctcgtagt gcgaggccag
     2581 gaattcgaac tggcgacgga agtcgccggg cggcggttgc gaggtcttcg gcggttcgcc
     2641 gccgcgcgcg atctcggcgt agaactcttc ggggctgagc ccaagcttgt cgaggtagct
     2701 ccggtcgccg aagtgcacgc gcaccggcac catgtggatg ccgaggcggt ccatctcgtc
     2761 ctcgggcagg tccgctgcgg aatcgacgac gaccgcgacg tggcgctccg cgcgatgcgc
     2821 cgcgtgctgc tgccgctgca tgtcgtccgc cttctgcccg gacacgacgc cgaaccgggc
     2881 ggcgaccctg aacacctcgg ccggatcgtc gacgtgcacg tgcacgcgca ccttgtgctg
     2941 cagtcccgcc acgaccaggc tgcccccgag cgtcgacagc gcctcgcgca ggtgccgacg
     3001 gtcgacgtgg ccaccggtca ccacgcactc cgtgcagtag cgccacgcga ggtcttcctc
     3061 ggtacccgcc gccacctcac tggaggcatc gagcacgacc accgcgtcgg caccgtcggg
     3121 cggcgccgtc tccgcgccgc tctcgaggaa gtcgctcaag ccggccacga gttcgacgaa
     3181 gccctgcgcg ccggcgtcga cgacattggc cctgcgcaac gcctcgagct ggaaccgcgt
     3241 ctgctcgagc gacgcctgtg ccgccacgac gccgcggcgg aaaagcgacc ggaaatcggc
     3301 cagtccgtcg cgtcgcgcac gctgcacctc gcgtgcaaag tcggtcagca cggtcaggat
     3361 cgtgccttcg cgcgggtcgg tcacgctttc gcgggcatag tcggcgccgc cggccacgcc
     3421 ttccacgaag tcgtcggggt cgagccgggt caggtgaccg agccggtcgc cgagcccgag
     3481 gaagaactgc gcgaggatcg cacccgaatt gccgcgggca ccgtcgagcg cggcgtcggc
     3541 gacgcgcgtg agcgtcttgc cggcgtgcgg atcgggcaac cgccgcagcg tcgccagcac
     3601 ggcccgcgcc gtgagcgcga ggttggtgcc ggtgtcgccg tcgggcaccg ggaagacgtt
     3661 gatcttgttg aggtgctcct gcttcgagag cagccgcagg atgccggcct gcagcgcgaa
     3721 gttcagccgc aacccgtcga gagtgccggc cggcgccgct acagcgttca cgcgcgcgcc
     3781 gtcccgctca gtcgttggtg agcttgaacc gggcgccgcg gtacgagagc accgcgcccg
     3841 tcgccgtgat ctgctcgacg atcggcccct cctgcaacgc ctcgccttcg cggtacttgc
     3901 gcgagttgac gaagatgaag cgctgctgcg gcgtgttcgc gtacacgtgc aggtcgaggt
     3961 gcagttcggg caatccgcca cgcgccgaga cctcgtccgc ggtcggcagg ttgtcggccg
     4021 ccggggccgg tgcggcaggc ggcggcgcgt acgacaggtc ctccgcctcg gggatcggcg
     4081 catagacgac ggtgccgccg cgtcgggtcg gtgcggcctg gcgagtcacg gcaggcgggc
     4141 ccggcggagg ggccgatgcc gccgcgacga gggacgggtc gaccgcaccg ccgacctcct
     4201 ccgcaagcgg gttgcgcccg cccgcggcgg gtcgcggcgc cgagtcgcgg gccaccggcg
     4261 tcgtcactgg cgggggcggt tcggacgaca gcgtcggttc agcggcggag ggagtggccg
     4321 gaggcgccgc cgcgtcggcg accggcctcg ccggagccgt cgtgaccggg gcgctcgccg
     4381 cggccgcggg cggtgtctcg tcgttcgcgc gccgcaacag cagcacgccg accgcgacca
     4441 ggtttacgac cagcagcgca atcgcggccg tcgcccagac gttggacttc ggcttcgtgg
     4501 cgacgaccgg cacctcggcc aacccgggac cggtctgctt ctgccgcgcg tgctcggact
     4561 tgcgcagtgc gtcgaggatg aacgacacgt cagcctccga cgctcgcagc gcggtcgtcg
     4621 ccgagcgtcg gcgagccggt cgggttcagg acggtgtcga gcacgatctg cgtctgcacg
     4681 ccggcgacgc cgtccgcgtt gagccgatgc tggcgctgga agtcctgcac gagtcgcgtg
     4741 agctcctcgt cgtagacgtc gttgccgggc gcggcctgcg gcagcccctg tgccacgcgg
     4801 aggttctcgc gcagccactt cacctcggcg ccgcgcatgc ccggcacgag cgacttgacc
     4861 accgcgagcg gcggtcgcca cagcagcagg aagtcgccga accagtagcg cgagatcgcc
     4921 gcgatcggta ccgcgagggc gctgccaccc agatcgatcg tcgccgcctc gtcgtcgagg
     4981 cccacgagca agacctggtg cgtgctgccg aggtcgtcgg tcagcgtcag gatcgccggg
     5041 cggttcagcg tgcgcagctg tgcccatgag cccttctgga acaggcactc gaggccctgg
     5101 gccgtcgcct ggtcgcagcc acgaccgcga ttcgcggcat acgccaggtt ccagcgggcg
     5161 aagagcttcg agagggcggc ctcggtcgtc gtgtcgttcg cgttctgcac cagcagctca
     5221 tcgagcgctg gcgccggcgg agcctgcgcg gcgtcgaccg tcgcgtcggc ggcggccggc
     5281 gccgggtcga ccggtgcggc gggcgcagcc gcggtcgcat cgctggcggc cgtcggcgca
     5341 ccggcgcggg tcgcaaacag cgtccatgcg ccgacgccca cgagcgcgag cgcggccgtc
     5401 acggcaccga tcgtcgccca gcgcagccac ggcgccggga cgggtcgacc gtagacttcc
     5461 gcggcagcca gccgcacgta cgcgggacct acgcgatggt cctcgcgcgt gaaggcgccg
     5521 agcagcgcgc ggtcgcagac cacgttgatc acgcgcggga tgccgccact caggcggtgg
     5581 acctccttga gcgcgcccgg cgtgaacgcc tcggccgtcg cgccggcgac gcgcatgcgg
     5641 tgcttgacgt agccggcggt ctcgtcacgc gacagtgggc tcaggtgata gcggccggtg
     5701 atgcgctgcg caagctggcg caggtccggc tgctcgagca gcgtgcgcag ttcctcctgg
     5761 ccgatcagga tgatctgcag cagcttggtc gtggccgtct cgaggttggt gagcaggcgc
     5821 acctgctcga gcgtctgcgg gctcaggttc tgtgcttcgt cgacgatcag cacgacgcgg
     5881 cggccccgcg cgtgcgcttc gagcaggtag tgcccgagca ggtccatcag cgccttggtg
     5941 ctgccgcgcc cttgctcggg cacctgcagg tgcagctcct cgcagatcgt cagcaggaac
     6001 tcggcgggcg tgacccgcgg attcaggatc agcgccactt ccgcgtgacc cggcaactgc
     6061 tcgagcaggc tgcggatcac cgtggtcttg ccggtgccga cctcgcccgt cagctggatg
     6121 aacccgccgg cctcgttgat gccgtacagc aggtgggcca gcgcctccgc gtgccgctcg
     6181 ctcaggtaga ggtaacgcgg gtctggcgtg atcgcgaacg gtttttcctg gaggccgaaa
     6241 aagctcgtgt acatgcggag ttaggtcgcg cggcggaaac tccatcatgc atcagggact
     6301 cgtcgaggga tagcgctgga ccccttccag actcgtttcg ccgccacccg cgtcaccacg
     6361 caccacgctg aacgacccgt cggtctccag cacgacggcc tcggcctctt cgaggcacga
     6421 gactcccgcc gcccgaacgg cagcccggac ttcctctttg gcgacccgcg cgcttcgcag
     6481 cgcgtccggc aggaattccc cccggtagag cagcagttgc ggctcgcccg tgaccagccg
     6541 ccggacccat cgcgtccgga cgctggtcca ggtgacgacg aactgcagtc cgatcagcaa
     6601 cgcgaaggcc agcacgccgt ccgccagcgc gatgttcgtg tcgaggagca cggcagagag
     6661 cgtcgaaccc agg
//
LOCUS       DASPFR010000151         2343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1266784, whole genome shotgun sequence.
ACCESSION   DASPFR010000151 DASPFR010000000
VERSION     DASPFR010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266784"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..581
                     /locus_tag="VD737_03830"
     CDS             165..581
                     /locus_tag="VD737_03830"
                     /inference="COORDINATES: protein motif:HMM:NF042761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HXW09718.1"
                     /translation="MRYIKFLLLGIIFGIALYKAEIVSWFRIQEMFRFDAFHMFGIIG
                     SAVVTASLVLTLLARVGARDRDGAPVELEPKELGTGIRYIAGGTIFGVGWAFTGACPG
                     PLVALVGAGVPAMIITLVCALAGTWAYGVLRPRLPH"
     gene            681..1877
                     /locus_tag="VD737_03835"
     CDS             681..1877
                     /locus_tag="VD737_03835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXW09719.1"
                     /translation="MARWLRLIARYVPGPVAPGYPGLPGLALAWTAVGLVAYARHFVA
                     GTPDIAVAGVLPGALMGLSCYLPWALLTAIVFDVERQVPLTRANWPRGLLLLAAVSIP
                     VVYAAWGLTIVFAGMTSSLSGLPQKAHGFGLVIPAHELLGHQLLFWASVGAAWMLRTL
                     LEARESERRASRLMLEKSQLETSLRQTELDALRMRLQPHFLFNSLQNIAVLTEHDAVT
                     ARRMLTKLADLLRASLGRDNRPTTTVAGEIELTSAYLAIERMRFGDRLSTLLDIAPDT
                     ASARVPSFLLQPIVENAIRHGLRGVQERGVIVVRSRREHGTLVLTVTDNGVGPGDAVA
                     DLPLGIGLGATCERLARMYAGAHSFSMTAVPEGGTEVRITLPFETDVTPQLEESRARA
                     ASAGRR"
     gene            1846..>2343
                     /locus_tag="VD737_03840"
     CDS             1846..>2343
                     /locus_tag="VD737_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXW09720.1"
                     /translation="MPALRVLVVDDEPLIRLGIRRALEAVPDVVVATECGTGPEAIAA
                     LSSGAHDLVVLDVQMPGCSGLDVVRAVGPSRMPAVIFLTAFDEYAVQAFEVNAVDYVL
                     KPFEPERLATAVARARDRVAMRAEGTLATRLEALLSARREPALERLVVRDGERYELVP
                     VDAIDW"
BASE COUNT          325 a          827 c          772 g          419 t
ORIGIN      
        1 cgtaaatggt cttgcgtagc tcgggtccga cttagctaac tccccagccg ccccggccaa
       61 tcgtaaatcg taaatcgtaa atcgtaaata cacctgccac ccacagctat tttccttagc
      121 caagggtgca atcgtaaatc gtaaatcgta aatcgtaaat ccaaatgcgt tatataaaat
      181 ttctcctctt aggcatcatc ttcggcatcg ccctgtacaa ggcggagatc gtatcctggt
      241 tccgcattca ggagatgttc cggttcgacg ccttccacat gttcgggatc atcggctcgg
      301 ccgtggtcac cgcctcgctg gtgctcaccc tgctcgcgcg cgtcggcgcg cgcgaccgcg
      361 acggcgcgcc cgtcgagctc gagccgaagg agctgggcac gggaatccgc tacatcgctg
      421 gcgggacgat cttcggcgtc ggctgggctt tcacgggtgc ctgccccggg ccgctcgtcg
      481 ccctggtcgg cgccggcgtg ccggcgatga tcatcaccct cgtctgcgcg ctggccggca
      541 cctgggccta cggcgtgctc aggccgcgtc tgcctcactg acattcgcca gccatttcgt
      601 gcggctcacc agcccgcggc tgccgttcgc cctgacggcg cggacctcac gggaagccgt
      661 gggagaatgg cgtttccttc atggctcgct ggctgaggtt gatcgcccgt tacgttcccg
      721 gtccggtcgc tcccggctac ccgggcctgc cgggtcttgc gctcgcgtgg acggccgtcg
      781 gcctcgtcgc gtacgcgcgg cacttcgttg ccggcactcc ggacatcgcc gtcgccggcg
      841 tgctgcccgg cgccctgatg gggttgtcgt gctatctccc gtgggccctt ctcacggcca
      901 tcgtcttcga cgtcgagcgg caggtgccgc tgacgcgagc gaactggccg cgcggccttc
      961 tgcttctggc cgccgtcagc atcccggtcg tctacgccgc ctggggcttg acgattgtct
     1021 ttgcgggcat gacgtcgtcg ctgtcgggac ttccgcagaa ggcgcacggc ttcggcctgg
     1081 tgatcccggc ccacgagttg ctcggccacc agctgctctt ctgggcgtcg gtcggtgccg
     1141 cctggatgct gcgcaccctg ctcgaggcgc gcgaaagcga acgtcgcgcc tcgcgcctga
     1201 tgctcgagaa gtcacagctc gagaccagcc tgcggcagac cgagctcgac gcgctgcgca
     1261 tgcggctgca gccgcacttc ctcttcaaca gcctgcagaa catcgccgtg ctgaccgagc
     1321 acgacgccgt caccgcgcgg cgcatgctga cgaagctcgc cgacctgctg cgcgcgtcgc
     1381 tcggacgcga caaccgcccg acgacgacgg tggcgggaga gatcgagttg acatcggcgt
     1441 acctggccat cgaacggatg cgttttggcg accgcctgtc gacgctgctc gacatcgcgc
     1501 ccgacaccgc gtcggctcgg gtgccgagct tcctgctgca gccgatcgtc gagaacgcca
     1561 tccgccacgg gctccgtgga gtgcaggaac gcggcgtcat cgtcgtccgc agccgacgcg
     1621 agcacggcac gctggtgctg accgtcaccg acaacggcgt cggccccggc gatgcggttg
     1681 ccgacctgcc gctcggcatt ggcctgggag cgacctgcga gcggctggcg cgcatgtatg
     1741 ccggcgcgca cagcttctcg atgacggccg tgcccgaggg cggcaccgag gtccgcatca
     1801 ccctgccgtt cgagaccgac gtgacacctc agctcgagga aagccgtgcc cgcgctgcga
     1861 gtgctggtcg tcgatgacga gccgctgatc cggctcggca tccgtcgcgc gctcgaggcc
     1921 gtgcccgacg tggtcgtggc caccgagtgc ggcacgggcc ccgaggccat cgcggcgctg
     1981 tcgtccggcg cgcacgacct ggtggtgctc gacgtccaga tgccgggctg ctcggggctc
     2041 gacgtggtgc gcgccgtcgg gccgtcccgt atgccggcgg tgatcttcct caccgccttc
     2101 gacgaatacg cggtccaggc attcgaggtt aacgccgtcg actacgtcct gaaaccgttc
     2161 gagccggaac gcctggcgac ggcggtggcg cgcgcgcgcg accgagtcgc gatgcgcgcg
     2221 gaaggcaccc tcgcgacgcg gctcgaggcc ctgctctccg ctcgccgcga gccggcgctc
     2281 gagcgactgg tcgtgcgcga cggcgagcgc tacgagctgg tgcctgtcga cgccatcgac
     2341 tgg
//
LOCUS       DASPFR010000152         2050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1632715, whole genome shotgun sequence.
ACCESSION   DASPFR010000152 DASPFR010000000
VERSION     DASPFR010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632715"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..524)
                     /locus_tag="VD737_03845"
     CDS             complement(111..524)
                     /locus_tag="VD737_03845"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXW09721.1"
                     /translation="MLRTITTRWMDNDQYGHVNNVVYYSYFDTAVNGYLIEASGCDVR
                     ELRQIGIVAETSCRFLRELSFPDVVHAGLALEKLGNSSVVYRIGLFRNDELEPAALGR
                     FVHVYVDSETRRPVPVPGEVRRALERLVQWPVVGG"
     gene            696..1823
                     /locus_tag="VD737_03850"
     CDS             696..1823
                     /locus_tag="VD737_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HXW09722.1"
                     /translation="MKIKAAVLRELGTPGPYAQTQPLAIEEFELAPPGPGEVLVRVRA
                     AGLCHSDLSVIDGNRPRPMPMVLGHEMAGVVEALGHGVTDLQRGDHVVASFVPSCGVC
                     VPCVAGRPALCEPGFASNTAGTLLSGERRLEQHGRPVNHHLGVSGFAEYATVSRRSLV
                     RVDPSLPFAEAALFGCAVITGVGAVVNTASMPKLSSMAVIGLGGVGLASLLAGSMLEA
                     NPIIAIDLHEAKLAKALELGATAAVNAGDPDCAERVRELSGGGVAYAFEMAGSARALE
                     LAYRITRRGGTTVTAGLPHPQQQVSIQHVTLVAEERTLRGSYLGSCVPERDIPQYIEW
                     YRAGRLPVDRLLSERITLDGINAAFDRLAAGASIRQVIEIG"
     gene            1863..>2050
                     /locus_tag="VD737_03855"
     CDS             1863..>2050
                     /locus_tag="VD737_03855"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HXW09723.1"
                     /translation="MDRAVARRLERWRDAGLVDEATAERIRRWETEHAAPEGSRLGRL
                     AFGFGGLLLASGLLLFVAA"
BASE COUNT          297 a          714 c          721 g          318 t
ORIGIN      
        1 aagaagtggc cagtggtcag tggctagtgg ttagtgaaga aagcagggtg tgcgtggcct
       61 cctctcctgc ctttactagc cactagccac tgaccactag ccaccagcca ctagccacca
      121 accactggcc actggaccaa ccgctccagc gcccgcctca cctcccccgg caccggcacc
      181 ggccggcgtg tctcgctgtc gacgtacacg tgcacgaagc gcccgagcgc cgcgggctcg
      241 agctcgtcgt tgcggaacag gccgatgcgg tacacgacgc tcgagttgcc gagcttctcg
      301 agcgcgagac ctgcgtgcac gacgtcgggg aagctcagtt cgcgcaggaa gcggcagctg
      361 gtctccgcga cgatgccgat ctggcgcagc tcgcgcacgt cgcagccgct cgcctcgatc
      421 aggtatccgt tcacggcggt gtcgaaataa gagtagtaga cgacgttgtt gacgtgcccg
      481 tactggtcgt tgtccatcca gcgcgtggtg atcgtccgca gcacgcgaaa ctccggacga
      541 cggctgggca gctcggcgcc gtccgcagtc ccgggcgcgc gaacggtgtc caatgtctgc
      601 tcactcatcg ggaacctcac gcccgcgcac gtcagcgggc cctggtcgca ttattgcagt
      661 ggagcggcaa tgggcgcttg ggctagtctc gacgcatgaa gatcaaggcc gccgtcctca
      721 gggagctcgg caccccgggc ccgtacgcgc agacgcagcc gctcgcgatc gaggaattcg
      781 agctggcacc gcccggcccc ggcgaggtgc tcgtgcgcgt tcgcgcggcc gggctgtgtc
      841 actcggactt gtccgtgatc gacggcaacc ggccgcgacc gatgccgatg gtgctcggcc
      901 acgaaatggc aggcgtcgtc gaggcgctgg gacacggcgt cacggacctg cagaggggcg
      961 atcacgtcgt cgcgtcgttc gtgccgagtt gcggtgtctg cgtcccgtgc gtcgctggac
     1021 gccccgcgct gtgcgagccg ggtttcgcgt ccaacacggc cggcaccctg ctctccggcg
     1081 agcgccggct cgagcagcat ggtcgccccg tcaatcatca cctcggcgtc tccgggttcg
     1141 ccgaatacgc caccgtctcg cggcgctcgc tggtgcgcgt cgacccgtcg ctgccgttcg
     1201 ccgaggccgc cctgttcggc tgcgccgtga tcaccggcgt cggcgcggtc gtcaacacgg
     1261 catcgatgcc caagctgtcg tcgatggcgg tgatcggcct cggtggcgtg gggctggcgt
     1321 cgctgctcgc gggcagcatg ctcgaggcga acccgatcat cgcgatcgac ctgcacgagg
     1381 cgaagctcgc caaggcgctc gaactcggcg cgacggccgc cgtcaatgcg ggcgaccccg
     1441 actgtgccga gcgagtgcgc gagctgagcg gaggcggcgt cgcctacgcg ttcgagatgg
     1501 cgggctcggc gcgcgcgctc gaactcgcct accggatcac gcgccgcggc ggcaccaccg
     1561 tgacggcggg cctgccgcac ccgcagcagc aggtgtcgat ccagcacgtc acgctcgtgg
     1621 ccgaggaacg tacgctgcgc ggcagctacc tcggaagctg cgtgcccgag cgcgacatcc
     1681 cgcagtacat cgagtggtac cgggcaggac ggctgcccgt cgaccggctg ctgagcgaac
     1741 gcatcacgct cgacggcatc aatgccgcgt tcgaccgtct cgcggccggc gcgtccatcc
     1801 ggcaggtgat cgagattggc tgagcaggcg ccgaccgcgc cgtcgtgggg gacgggcggc
     1861 tgatggaccg cgccgtcgcc cgacgcctcg agcgctggcg cgacgccggg ctcgtcgacg
     1921 aagccaccgc cgagcgcatc cggcggtggg agacggagca cgcggcgccg gaaggttcgc
     1981 gcctcgggcg gctggcgttc ggcttcggtg ggctgttgct ggcgagcggc ctgctgctgt
     2041 tcgtcgcggc
//
LOCUS       DASPFR010000153         8299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_411474, whole genome shotgun sequence.
ACCESSION   DASPFR010000153 DASPFR010000000
VERSION     DASPFR010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8299
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411474"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /gene="bioA"
                     /locus_tag="VD737_03860"
     CDS             <1..1192
                     /gene="bioA"
                     /locus_tag="VD737_03860"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:TIGR00508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HXW09724.1"
                     /translation="CTHEPAVALAERLIRLAPAGLTRCFYADNGSSAIEVAVKMSFHY
                     WRNRGHVRKRRFITLANSYHGETLGALAVGHVELYREIYQPLLMEGVITAPSPDAWAA
                     APGETPEDCARRAAAAMAELLERHGAETCAVIVEPVVQCAAGMRMHSPLYLTLLRELC
                     DRHDVHLIADEIAVGFGRTGTLFACEHAAVTPDFLCLSKGITGGYLPLSVVLTRDEVY
                     EAFYDEYAQLRAFLHSHSYSGNALACAAALATLDIFESDAVLERNRALAARLGERARA
                     LLGDLPHVAQVRQAGMIVAAELARHGDRRDPFDWRERRGVQVYRHALGHEALLRPIGN
                     VVYFMPPYVITPEQIDTLVATARDSILEATADCPARPSRRVPAMQPPSAAATPAASST
                     GLEF"
     gene            1200..1934
                     /locus_tag="VD737_03865"
     CDS             1200..1934
                     /locus_tag="VD737_03865"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HXW09725.1"
                     /translation="MRITRVYVDSPLTPDTDVRLPESAAYHVARVLRLREGATITAFD
                     GSGADFRCEILAVKGDEVRVHVGARSTGLAESPLRITLVQAVSRSERMDWTLQKATEL
                     GVRTIVPVLSARSVVRLDERQAEKKMRHWQAIVAAACEQCGRSVVPEVRVPQDLGRYL
                     ASPVREGQRLVLSPTGPASLAGLASVGNRVELLIGPEGGLEDSELERAAASGFTPVRL
                     GPRILRTETAGIVALTVLQSLWGDLG"
     gene            complement(2030..3115)
                     /locus_tag="VD737_03870"
     CDS             complement(2030..3115)
                     /locus_tag="VD737_03870"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXW09726.1"
                     /translation="MSRSSERVMQIRQHVRYTTASDGARLAWAEAGSGAPIVKAANWL
                     THLEYEWASPLWKHWLQYFSGIGRLVRYDERGCGLSDWNRVPLTLDQWVEDLDSVIEA
                     ARPAGPVTLLGISQGAATCISYAVRRPERVARLVLYGGYAHGAAHRGTPADQAAYRAM
                     IDLARAAWDRENSSFRQVFTSRFIPGASAGQLAWFNDLCRKCSTGEVVAELLQARSTV
                     DVSGLLGQVRVPTLVLHARGDEVIPVSEGRLLAASIPGAEFVELDSRNHVLLEHEHAW
                     SRFTDAVSAFVGGSRDADSPFGALSAREREVLAAIAEGLRNAEIAERLNISEKTVRNH
                     ATVVFEKLGVTSRAQAIVFARDRHFGG"
     gene            3220..3990
                     /locus_tag="VD737_03875"
     CDS             3220..3990
                     /locus_tag="VD737_03875"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXW09727.1"
                     /translation="MLERTFQFLYGVACYVVFLATFLYAIAFVGGFLVPTPLDAPAGS
                     TPLAQAILIDALLLLVFAVQHSVMARPWFKARWTRIIPPAVERSTFVLAASLALALVF
                     WQWRPIGITVWSVDEPVGRALLWVLFAAGWGTVLVVTFLISHFDLFGLRQVWLSLRGQ
                     PYTPVAFRTPLPYRHVRHPLYLGFMLAFWATPHMTVAHLLFALLTTGYILVAIQFEER
                     DLVAQHGARYEHYRRTVPMLVPRWRREMRASAIDRGHA"
     gene            complement(3994..4488)
                     /locus_tag="VD737_03880"
     CDS             complement(3994..4488)
                     /locus_tag="VD737_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HXW09728.1"
                     /translation="MPNTTFTPTTPTTPGDEIYCLLVPLADDRLLLPRACVTEVINYQ
                     APTPMDDVPPWYLGTIAWGGQRVPLVSFEATLGKALPRASGRTRIVVMQGLTGRGGGH
                     FAILTQGFPQLVRVSPEIVHTDDSRSFNERMPVIGQVRMVNEYPLIPDFERLEEMIDD
                     ETRA"
     gene            complement(4505..>8299)
                     /locus_tag="VD737_03885"
     CDS             complement(4505..>8299)
                     /locus_tag="VD737_03885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXW09729.1"
                     /translation="IESLLNRVISQTLARSPDIVAVVRDAVAAMPQLVDEIDGGAPAT
                     IDVVAIMSRADSISGREGPSLPGTATTPPAPATPSTVEEVDAPEPVAAAPTEEPAGMD
                     PVLRDIFRKETAGHILVVREYLDRCSRGVAPHPVTEALYRACHTLSGIAKTAGARQGI
                     KVAEPMEHYVRKLHDNGHGLDAEALRLLSETVHVLETVAENVDEDTGFFPEQGRLVEG
                     WIALDRALDAELARLADAAERTLGDAWRPAEPRPAPVASAPPPPLADAPAQDFAATST
                     PTPEDLTEPSLPSLADDALRAADLDIDYTTPHEALPDHGERVPAAGTSPAEPESPPDE
                     AVTAPPHLEPAGEDDEFDADIAAIFGEEATELLEQADAAFARWRADRADRSQVTELKR
                     LLHTLKGGARMAGIRSMGDLSHELESFLAVVETSAARHDDAPTFDVLQSSLDELHRMR
                     EMANTGLRIAPATGLMERIRASASALPGAAAEASASGAERAEAARAEAPELATDEAAT
                     TGASPTVGGEEIVVEFEAVTPAPESLVEALLRDEEATEAAVPVEGIEVFEVAAIEEVE
                     VGAAEPTALGDEAVPPPEAVAPVEEHRYVDEVEPEPATELPPPIGVDTAAEFEIPPAR
                     PMPPAEAEPESPFEIAATYSQPVLPGREPVAPTERSELARVDADLLDDLLNNAGEVSI
                     FRARIEQQMTSMEFNLAELDRTVTRLREQLRKLELETEAQILHRHQADEAAHRTDFDP
                     LELDRYSTIQQLSRALAESVSDVASIEGLLANLNRDTQNLLQQQGRVVTEVQNGLMRT
                     RMVPFQRHVQRLTRIVRQAATEAGKKAELVVEGATGELDRQVLERMLPPFEHMLRNSI
                     VHGIEAPSQRELAGKPEAGRITMKLQREGAEVVIVVEDDGAGLNVPAIRAKARQMGLL
                     QPEQDLTDEEALQLVLEPGFSTASRLTQSAGRGVGMDVVATEVKKLGGGLFIESKPGY
                     GARFTIRLPFTLAITQALIVRVHDELFALPMATVEGVARLTRTEIERHLGEEHPTFEY
                     GGTAYRFQHLGTFVGSGPSLLPETDAALPVILVRAGEHSTALVTDELVGSREIVVKPV
                     GPQIASIRGISGATILGDGRIVIILDMGTLVRSEWRARAAEGPRPLRDDRIFALVVDD
                     SITVRRVTQRLLERNGMRVMTAKDGVEALALLQDHVPDVVLLDIEMPRMDGYEVAGHV
                     RSDARLADVPIIMITSRVGDKHRARAIELGVDDYLGKPYQESQLLDAIEPLVLARRGQ
                     QG"
BASE COUNT         1111 a         2909 c         2959 g         1320 t
ORIGIN      
        1 ctgcacgcac gagcctgccg tggccctcgc ggagcgcctc atacggctgg ccccggccgg
       61 gctcacgcgc tgcttctacg ccgacaacgg ctcgagcgcg atcgaggtcg cggtcaagat
      121 gagcttccac tactggcgca accgcggaca cgtgcgcaag cgccgcttca tcacgctcgc
      181 caattcctat cacggcgaga cactgggcgc gctggcggtg gggcacgtcg aactctaccg
      241 ggagatttac cagccgctgc tgatggaggg cgtgatcacc gccccgagcc ccgacgcctg
      301 ggccgccgcg cccggagaga cgccggagga ctgcgctcgc cgggccgcgg cggccatggc
      361 cgagctgctc gaacggcacg gcgcggagac gtgtgcggtg atcgtcgagc ccgtcgtcca
      421 gtgcgcggcg gggatgcgga tgcactcgcc gctgtacctc accctgctgc gcgagctgtg
      481 cgatcggcac gacgtgcacc tgatcgcgga cgagatcgcc gtcggcttcg ggcgcaccgg
      541 cacgctgttc gcctgcgagc acgcggccgt cacgcccgat ttcctgtgcc tgtcgaaagg
      601 catcaccggc ggctacctgc cgctttcggt cgtgctgacc cgcgacgagg tctacgaggc
      661 cttctacgac gaatatgcgc agctgcgcgc cttcctgcac tcgcacagct actcgggcaa
      721 cgcgctcgcc tgcgcggccg cactggcaac gctggacatc ttcgaatcgg acgccgtgct
      781 cgagcgcaac cgtgccctcg ccgcccgcct cggcgagcgg gcgcgtgcac tgctcggcga
      841 cctgccgcac gtggcccagg tgcggcaggc cggcatgatc gtggcggccg aactcgcgcg
      901 gcacggcgac cgacgcgatc cgttcgactg gcgcgagcgg cgcggcgtgc aggtctaccg
      961 tcacgcgctc gggcacgagg cgctgctgcg gccgattggc aacgtggtct acttcatgcc
     1021 gccgtacgtc atcacgccgg agcagatcga cacgctggtc gcgaccgccc gcgactccat
     1081 tctcgaggcc accgcggatt gcccggcacg accgtcgaga cgggttccag cgatgcagcc
     1141 gccgtccgcc gcggcgacac ctgccgcgtc gagcaccggc ctcgagttct gagcgacccg
     1201 tgcgcatcac ccgcgtctac gtcgactcgc ccctcacgcc ggacaccgac gtgcgcctgc
     1261 ccgagtcggc cgcctaccac gtcgcacgcg tgctgcggct gcgggagggc gcgacgatca
     1321 cggcgttcga cggcagcggc gcggacttcc gctgcgagat cctcgcggtc aagggcgacg
     1381 aggtacgagt gcacgtcggc gcgcgcagca ctggcctggc cgagtcgccg ctcaggatca
     1441 cgctcgtgca ggcggtgtcg cgcagcgagc gcatggactg gacgctgcag aaagccactg
     1501 agctcggcgt gcgcacgatc gtgccggtgc tgtcggcccg cagcgtcgtg cgtctcgacg
     1561 agcgccaggc cgagaagaag atgcgccact ggcaggccat cgtcgcggcc gcctgcgagc
     1621 agtgtggtcg cagcgtcgtg ccggaggtgc gcgtgccgca ggacctcggt cgttacctcg
     1681 cctcaccggt gcgcgagggc cagcggctgg tgctgagccc gacgggcccg gcctcgctcg
     1741 cggggctcgc cagcgtcggc aaccgcgtgg aactgctgat cggcccggaa ggcgggctcg
     1801 aggactccga actcgaacgc gccgccgcgt ccggattcac gccggtccgc ctcggccccc
     1861 ggatactgcg caccgagacc gccggcatcg tcgcgctgac cgtgctgcag tcgctgtggg
     1921 gcgacctggg ataggcgggc cgcaactcgg ttccaggacg gtcggagcgg cagcacgttt
     1981 cgcctgacgg caaaggtacg gcggatcgat tgggcgtcga ggtgcagctt cagccgccga
     2041 aatgacggtc gcgcgcgaac acgatcgcct gggcgcgcga cgtcacgccg agcttctcga
     2101 acaccaccgt ggcgtggttg cgcacggtct tctcgctgat gttcaggcgc tcggcgatct
     2161 ccgcgttgcg caggccctcg gcgatggccg ccagcacctc gcgctcgcga gccgacagcg
     2221 cgccgaacgg cgagtcggcg tctcgcgaac cgccaacgaa agccgacacg gcgtccgtga
     2281 atcgcgacca cgcatgttcg tgctcgagca gcacgtggtt gcgcgaatcg agctcgacga
     2341 actccgcacc cgggatgctg gcggccagca gccgtccctc gctgacgggg atcacctcgt
     2401 cgccgcgcgc gtggagcacg agcgtcggca cgcggacctg gccgagcagt ccagacacgt
     2461 cgacggtgga gcgcgcctgg aggagctcgg cgaccacctc cccggtggag cacttgcggc
     2521 acaggtcgtt gaaccacgcg agctgcccgg cgctggcccc gggaatgaag cgggaagtga
     2581 atacctggcg aaaggacgag ttctcacggt cccaggctgc gcgagcgaga tcgatcatcg
     2641 cgcggtaggc ggcctggtcc gcgggcgtgc cgcggtgtgc cgcgccgtgc gcgtagccgc
     2701 cgtagagcac gagccgcgcg acgcgctccg gacggcgcac cgcgtagctg atgcaggtgg
     2761 cggcgccctg cgaaatgccg agcaacgtga ccggccccgc gggccgtgcc gcctcgatca
     2821 ccgagtcgag atcctcgacc cattggtcga gggtcagcgg gacccggttc cagtcgctga
     2881 gcccgcagcc ccgctcgtcg tagcgcacca accggccgat gcccgagaag tactgcagcc
     2941 agtgcttcca gagcggactg gcccactcgt attcgaggtg ggtcagccag ttggcggcct
     3001 tgacgatggg tgcgccgctg ccagcctcgg cccacgcgag ccgcgccccg tcggaggcgg
     3061 tcgtgtaacg cacgtgctgg cggatctgca tgacgcgttc gctcgaccgg gacatccggc
     3121 ccacgtggaa gggcccgagt ctagaccagt cgggacgtac cgacgatggt gcgggcctgc
     3181 ggcacctgtg acgctcgtcg gcgtcaacgg aggcaagcca tgctcgagcg tacattccag
     3241 tttctctacg gcgtcgcctg ctacgtggtg ttcctggcga cgttcctcta tgcgatcgca
     3301 ttcgtcggcg ggttcctcgt gccgacgccg ctcgacgcgc cggcagggtc gacaccgctc
     3361 gcacaggcca tcctgatcga cgcgctgctg ctgctcgtct tcgccgtgca gcacagcgtg
     3421 atggcacggc cgtggttcaa ggcgcggtgg acccggatca tcccgcctgc ggtcgaacga
     3481 tcgaccttcg tgctggcggc gagcctcgcg ctcgccttgg tgttctggca gtggcggccg
     3541 atcggcatca cggtgtggtc ggtcgacgag cccgtgggcc gcgcgctgct ctgggtgctg
     3601 ttcgcggcgg gctggggcac cgtgctcgtg gtgaccttcc tgatcagcca cttcgacctg
     3661 ttcggtctgc ggcaggtctg gctgtcgctg cggggccagc cttacacgcc cgtggcattc
     3721 cgcacgccgc tgccgtaccg gcacgtgcgt catccgctct acctcggttt catgctcgcc
     3781 ttctgggcga cgccgcacat gacggtggcc cacctgctgt tcgcgctgct cacgaccggc
     3841 tacatcctgg tcgcgataca gttcgaggaa cgcgacctcg tcgcgcagca cggcgcgcgg
     3901 tacgagcact accggcggac cgtgccgatg ctggtgccgc gctggcggcg ggaaatgcga
     3961 gcgtccgcga tcgatcgcgg acacgcctga gcctcaggcg cgcgtctcgt cgtcgatcat
     4021 ctcctcgagg cgctcgaagt ccgggatcaa cgggtactcg ttgaccatgc ggacctgccc
     4081 gatgaccggc atgcgctcgt tgaagcttcg cgagtcgtcc gtatggacga tctcgggact
     4141 gacgcgcacg agctgcggga agccctgggt caggatggcg aagtggccgc cgccgcggcc
     4201 ggtcagcccc tgcatcacca cgatgcgcgt gcgaccgctg gcgcgcggca gtgccttgcc
     4261 gagcgtcgcc tcgaacgaca cgagcggcac gcgctggccg ccccaggcga tcgtcccgag
     4321 gtaccacggt ggcacgtcgt ccatcggcgt gggcgcctgg tagttgatca cctccgtcac
     4381 gcaggcgcgc ggcaacagca gccggtcgtc ggcgagcggc accagcaggc agtagatctc
     4441 gtcgcctggc gtcgtcgggg tggtcggcgt gaaggtggtg ttcggcatcg ggcggctccg
     4501 atcgtcagcc ctgctggccc cgccgcgcca gcacgagcgg ctcgatcgcg tcgagcagct
     4561 ggctctcctg gtagggcttg ccgaggtagt cgtccacgcc gagctcgatg gcacgggcgc
     4621 ggtgcttgtc gccgacgcgc gaggtgatca tgatgatcgg cacgtcggcg agccgcgcgt
     4681 cgctgcgcac gtgcccggcc acctcgtagc cgtccatgcg cggcatctcg atgtcgagca
     4741 ggaccacgtc cgggacgtgg tcctgcagca gcgcaagcgc ttcgacgccg tccttcgccg
     4801 tcataacgcg catgccgttg cgttcgagca gccgctgcgt cacgcggcgc accgtgatcg
     4861 aatcgtccac caccagcgcg aagatccggt cgtcgcggag cggtcgcggg ccttcagccg
     4921 cgcgggcacg ccactcggaa cgcaccagcg tgcccatgtc gaggatgatc acgatgcgac
     4981 cgtcgccgag gatcgtcgcg ccggagatgc cgcggatgct ggcgatctgc gggccgaccg
     5041 gcttgacgac gatctcgcgg ctgcccacga gttcgtcggt gaccagggct gtggagtgct
     5101 cgcccgcgcg caccagtatc accggcagcg ccgcgtccgt ctcgggcagc agcgacggcc
     5161 cgctgcccac gaaggtgccg aggtgctgga agcggtaggc ggtgccgccg tactcgaagg
     5221 tcgggtgctc ctcgccgagg tgccgctcga tctcggtgcg cgtcaggcgc gcgacgccct
     5281 cgacggtcgc catcggcagt gcgaacagct cgtcgtgcac gcgcacgatc agcgcctgcg
     5341 tgatcgcgag cgtgaacggc aggcggatcg tgaaccgcgc gccgtagccc ggcttcgact
     5401 cgatgaacag cccgccgccg agtttcttga cctcggtcgc cacgacgtcc atgccgacgc
     5461 cacggccggc cgactgagtg agccggctcg ccgtgctgaa tccgggttcg agcacgagct
     5521 gcagcgcttc ctcgtcggtg aggtcctgct ccggctggag caggcccatc tgccgggcct
     5581 tggcacggat cgccggaacg ttcaggcccg cgccgtcgtc ctcgaccacg atcacgacct
     5641 cggcgccctc gcgctgcagc ttcatcgtga ttcggccggc ctccggcttg ccggcgagct
     5701 cgcgctggga cggggcctcg atgccgtgca cgatcgaatt gcgcagcatg tgctcgaatg
     5761 gcggcagcat gcgctcgagc acctgccggt cgagttcacc ggtggcgcct tcgacgacga
     5821 gctcggcctt cttgccggcc tcggtcgcgg cctggcggac gatgcgcgtg aggcgctgga
     5881 cgtggcgctg gaacggcacc atgcgcgtgc gcatcaggcc gttctgcacc tcggtgacga
     5941 cgcggccctg ctgctgcagc aggttctgcg tgtcccggtt caggtttgcg agcagcccct
     6001 cgatgctcgc cacgtcgctg accgattcgg cgagcgcgcg cgaaagctgc tggatcgtcg
     6061 aatagcggtc gagctcgagc gggtcgaaat cggttcggtg cgcggcttcg tccgcctggt
     6121 ggcggtgcag gatctgcgct tcggtctcga gttcgagctt gcgcagctgc tcgcgcaacc
     6181 gggtgacggt ccggtcgagc tcggcgaggt tgaactccat cgaggtcatc tgctgctcga
     6241 tgcgggcgcg gaagatgctc acctcgcccg cgttgttgag caggtcgtcg agcaggtcgg
     6301 cgtcgacgcg cgccagttcg gaacgctcgg tcggcgcgac gggttcgcgc cccggcagga
     6361 ccggctgcga gtaggtggca gcgatctcga acggtgattc gggctccgcc tcggcgggcg
     6421 gcatcgggcg cgccggcgga atctcgaatt cggccgccgt atcgacaccg atcggtggcg
     6481 gcagttcggt cgcgggctcc ggctcgacct cgtcgacgta tcgatgttct tcgaccggcg
     6541 cgacggcctc cggtggcggg accgcctcgt cgccgagcgc ggtcggctcg gccgccccga
     6601 cctcgacttc ctcgatggcc gcgacttcga acacctcgat gccttcgacc gggacggccg
     6661 cttccgtcgc ttcctcgtcg cgcagcaggg cttcgacgag tgactccggt gcaggtgtca
     6721 cggcctcgaa ctcgacgacg atctcctcgc ccccaaccgt gggtgacgct ccagtcgtcg
     6781 cggcctcgtc cgtcgccagt tctggcgcct cggcccttgc agcttccgcg cgctccgcgc
     6841 cggaggcgga cgcctccgcg gccgctcccg gcagcgcgga ggccgatgcg cggatgcgtt
     6901 ccatcagccc ggtcgccggg gcgatccgca ggcccgtgtt ggccatttcg cgcatgcggt
     6961 gcagttcgtc gagggacgac tgcagcacgt cgaaggtcgg ggcgtcgtcg tgacgcgcgg
     7021 cgctggtctc gaccaccgcg aggaacgact cgagctcgtg gctcaggtcg cccatggatc
     7081 ggatgcccgc catgcgcgcg ccgcccttca gcgtgtgcag caggcgcttg agttcggtga
     7141 cctggctgcg gtccgcgcgg tcggcgcgcc agcgtgcgaa cgcggcatcg gcctgctcga
     7201 gcagttcggt cgcttcctcg ccgaagatcg cggcgatgtc ggcgtcgaac tcgtcgtcct
     7261 cgccggccgg ctcgaggtgt ggcggcgcgg tgaccgcttc gtccggcggc gactcgggct
     7321 ccgctggcga cgtcccggcg gccggaacgc gctcgccgtg gtccggcagc gcttcgtgcg
     7381 gcgtcgtgta gtcgatgtcg aggtcggccg cccgcagtgc gtcgtcggcg agggacggca
     7441 gcgacggctc ggtgaggtct tcgggcgtcg gcgtcgacgt tgccgcgaag tcctgcgcgg
     7501 gcgcatccgc gagcggtggc ggtggtgcgg aggcgacggg cgcaggtcgc ggctctgccg
     7561 gtctccacgc gtcgccgagc gttcgctcgg ccgcgtcggc gaggcgggcc agttcggcat
     7621 cgagcgcacg gtcgagcgcg atccagcctt cgacgaggcg accctgttcc ggaaagaagc
     7681 ccgtgtcctc gtcgacgttc tcagccacgg tctcgagcac gtggaccgtc tcgctcagca
     7741 gccggagggc ctcggcgtcg agcccgtggc cgttgtcgtg aagcttgcgg acgtagtgct
     7801 ccatcggctc ggcgaccttg atgccctggc gggcaccggc cgtcttggcg atcccgctca
     7861 acgtgtggca cgcccggtag agcgcttcgg tcaccgggtg tggcgcgacg ccccgcgagc
     7921 agcggtccag gtattcgcgc accaccagga tgtggccggc ggtctccttg cggaagatgt
     7981 cgcgcagcac ggggtccatg cccgcgggct cctccgtggg ggcggccgcg accggctccg
     8041 gcgcgtcgac ttcctcgacc gtcgacggcg tggccggcgc cggaggcgtg gttgcggtgc
     8101 ccggcagcga cgggccctcg cggccggaga tcgagtccgc ccgcgacatg atcgcgacga
     8161 cgtcgatcgt cgcgggcgcg ccgccgtcga tctcgtcgac cagctggggc atcgcggcca
     8221 ccgcgtcgcg caccacggcg acgatgtccg gcgatcgcgc aagcgtctgg ctgatcacgc
     8281 ggttgagcag cgattcgat
//
LOCUS       DASPFR010000154         8549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1144765, whole genome shotgun sequence.
ACCESSION   DASPFR010000154 DASPFR010000000
VERSION     DASPFR010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8549
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144765"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="VD737_03890"
     CDS             <1..1319
                     /locus_tag="VD737_03890"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXW09730.1"
                     /translation="AEPGEPAVRPRLAACLLFAGLVAGHGSALAQSTTAFVHATLYTL
                     EDAQPIEDATVVIRDGKVQAVGAGLSPPAGARVIDVEGRIVTPGLMSAGSQLGLVEVG
                     SLADTRDYAVASGALGAAFDVQYALNPNATTFDLALSDGLTRALTYPEGSSVAPFLGQ
                     AALLRLGSADALLERAQVAMFVRVGGGAAAGAGGSRSASWSLLRNALEEARRYRPVAG
                     VGRPRDQLLGRLDAEALQDVVRGRMPLAIVADRESDLRQVLRLHDDTGLPLIVYGGAE
                     AWRVAPALAARKIPVVLDPTLNLPASFDALGARLDNAALLHAAGGRVAFCMSAFFRTY
                     NAGSGLRFGAGLAVANGLPWIEGLRAMTSSPASIFGLDGRYGRVRPGADADLVVWDGD
                     PLEPSTSALQVWVRGVEMPVDATRQHALAERYSPLRKREMPPAYRH"
     gene            complement(1409..2194)
                     /locus_tag="VD737_03895"
     CDS             complement(1409..2194)
                     /locus_tag="VD737_03895"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXW09731.1"
                     /translation="MGARSTRGDDYQDVPRPVAALADEYPPNSYDPPHSHKRGQLVYA
                     ISGVLVCTTRDATFVVPPQRALWVPSGVVHEARTRGHVSLRTLYLDDAVTPGLPRTCM
                     TIEVSTLLRELIVEATRIPIEYDVDSRDGRVMTLIVDEIRAAPHTPLHVPMPRHPQLV
                     RICHAILKDPAQNDVLDDWAVAAGMSRRTFTRIFRSETGVTFATWRQNVRLIEAMSRL
                     STGQSVTAVALDVGYNSPSAFTAMFRRAFGVPPRQYFGTEETH"
     gene            2380..3393
                     /locus_tag="VD737_03900"
     CDS             2380..3393
                     /locus_tag="VD737_03900"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXW09732.1"
                     /translation="MSSNAGEAAVGHGAGDDAIDPDIRRFVARVTADYARLTGGRELP
                     VGEMRSVAEQVREPWREGGPVMASVTERVADTEAGPVRLRIYDPGVAKPAPALIYMHG
                     GGWVLFSLDTHDRVMREYAARAGVVVIGVDYALSPEARYPAALNQVVAVVRWLQTRGA
                     KLGVDARRLALGGDSAGGNLSICAALALRDAGERDLVRGLLLIYPAFDRHCSAESLRR
                     FGGPGAVLTAEEVDYFWDHYTGGVDVSNDPHAMPMRASLEGLPPVCMTIPACDVLTEQ
                     SHEMADRLRAAGVDVSMTVYPGATHSFIEAVSIAPLADRAIEDGARWVRRTLGAGAPT
                     GPR"
     gene            3407..4459
                     /locus_tag="VD737_03905"
     CDS             3407..4459
                     /locus_tag="VD737_03905"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HXW09733.1"
                     /translation="MKPALRKLHRWIALTLGVLWLSQALTGLVMVYRWELDDAALEAP
                     AVPLDAAALGARIEAIERDPAGLTVTSLWATGGVDGRFDLYVDDSAGDTDIVRVDGRG
                     RVLRSRGSGEGFVPTAASLHQTLLAGDRGKLVVGISGVFLLVTIVMGLVLAWPARARQ
                     WRPALLPKGARPGAARRYAWHRAGGLWIGIPALVSLGAGVLLTFESTLAGWLGTDATP
                     PELAGIEAPAGAPIAPAQAFTTALAQFPGSSLSGVSFPSQEQPWFRIRVRQPEEWRRV
                     YGTSVVYVAAADGTVLRTEDALQAPAATAFMSNLYPVHTGEALGVVGRVFALVVGAWL
                     LAMLVLGVGLWLARRR"
     gene            4464..6026
                     /locus_tag="VD737_03910"
     CDS             4464..6026
                     /locus_tag="VD737_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXW09734.1"
                     /translation="MRQDDPACSHGLGRLFARRSIESLQSDAQRSGLPRTLSAFNLVL
                     LGIGCIVGAGIYVLPGLAAAHFAGPGVVLSFLLAGAACALAALCYAELASTMPVSGSA
                     YAYCYAAMGEVFAWSLGCLLLFEYGIAAALLAVGFSGYLGSLLRDVGITMPAALSTPL
                     VQAEILPGGTRLHVGTSFNLVAALAIAGITAVLVRGVQASALVNNVLVLLKVTVLGGL
                     VVVGAAAVDPANWVPFVPPNEGGFAFGWQGVVRAASILFFAYIGFETVSTAAAEARNP
                     QRDLPIGILGSLAACTLIYVVVAAVLTGIVPYRELGVPDPIALAIDRLHMPVLASLVK
                     VGALAGLASVLLVNAFGQSRVAFAMSRDGLLPPLFSRLGARVRTPVAGVVLFAIVSAL
                     GAALLPLSLLGDLTSIGTALAFATVCLTVLWLRNARPDLHRPFRVPLGGFRIGGVWIG
                     WVPLGGMILCLGMAAPVAIDVTGQALRGQVLPVLIIAGYAAACTLAYFGYAVRNSSLR
                     RVGATIVEPGAP"
     gene            6104..7732
                     /gene="pruA"
                     /locus_tag="VD737_03915"
     CDS             6104..7732
                     /gene="pruA"
                     /locus_tag="VD737_03915"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR01236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HXW09735.1"
                     /translation="MKHSSNLPPPRNEPIQDYAPGSTQRARLKAAIADVEAAASEIPG
                     FVGGRTVHGGTPVRVTSPHRHERAVAQLHQLGADEVRQAIEAAVEAQHDWAALAFEQR
                     AAVFLRAADLIAGPWRDRMNAATMVGQSKTVVQAEIDAACELADFLRFNVHYAERLLT
                     EQPESSPGTWNRVDYRPLEGFVYAISPFNFTAIGGNLCTAPAILGNTVVWKPATTASL
                     SNWRIMEVLREAGLPDGVINFVPGDAVTITREVLADPRLAGVHFTGSTSVFQSIWREV
                     GERIDRYRTYPRLVGETGGKNFVVAHPSADADSLAVALVRGAYEYQGQKCSAASRAYV
                     PASLWPELRYRLLSEISSIRVGDPKDFRNFMGAVIDRRAYDRIHGYLERAAADTDVEV
                     LTGGTGDTSEGWFIEPTLVQTSDPAHRLMREELFGPVLAVYVYDDSEWREVLALVDRT
                     AEYGLTGAVFARDRAAIVEAATVLRNTAGNFYVNDKPTGAVVGQQPFGGARASGTNDK
                     AGSILNLWRWVSPRTIKENFVPPRDVRYPSMGEE"
     gene            7891..>8549
                     /locus_tag="VD737_03920"
     CDS             7891..>8549
                     /locus_tag="VD737_03920"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HXW09736.1"
                     /translation="MSSGTHTTRNLRLAIGCVLALASAAGSAQQAASDQELEEIIVTG
                     SQIRLPDPFAGGQVAEGGRAGILGNLTTLETPFSSTNYTEELMRNQQAKSVADVLLND
                     PNVRAARGFGNFQELFMIRGFPAYSDDMTYNGIYGILPRQFVASEYMERVELFRGASA
                     FLNGAAPGGSNLGGTVNLVPKRAPAEGVRRLTAGWESDSAYYLAGDFGRRFGPDDQTG
                     VR"
BASE COUNT         1129 a         3002 c         3087 g         1331 t
ORIGIN      
        1 cagccgagcc gggagagcct gccgtgaggc cacggcttgc ggcctgtctg ctgttcgccg
       61 gactggtcgc gggccacggg tccgcgctcg cgcagtcgac caccgcgttc gtgcacgcca
      121 cgctgtacac gctcgaggac gcgcagccga tcgaggatgc gacggtggtg atccgcgacg
      181 gcaaggtgca ggccgtgggc gcgggcctgt cgccaccggc cggggcgcgc gtgatcgacg
      241 tcgagggccg catcgtcacg ccgggcctga tgagcgcggg ctcgcagctc gggctggtcg
      301 aggtcggcag cctcgccgac acgcgtgatt acgcggtcgc cagcggcgcg ctcggcgccg
      361 cgttcgacgt ccagtacgcg ctgaacccga acgcgacgac gttcgacctc gcgctgtccg
      421 acgggctcac gcgcgcgctc acgtatcccg aaggctcgtc cgtggcgccg ttcctcgggc
      481 aggccgcgtt gctgcgcctc ggatccgccg acgcgctgct cgagcgcgcg caggtcgcca
      541 tgttcgtgcg cgtcggcggt ggtgcggctg ccggcgcggg tggttcgcgc agcgcctcgt
      601 ggagcctgct gcgcaacgcg ctcgaggagg cccgtcgcta tcggcccgtc gcgggcgtcg
      661 ggcgcccgcg cgaccagctg ctcgggcggc tcgacgccga ggcgctgcag gacgtcgtca
      721 ggggccgcat gccgcttgcg atcgtcgccg accgcgaatc ggacctgcgc caggtgctgc
      781 gactgcacga cgacacgggc ctgccgctga tcgtctacgg cggcgccgag gcctggcgcg
      841 tcgcaccggc gctggccgcc cggaagatcc cggtcgtgct cgatccgaca ttgaacctgc
      901 cggcgtcgtt cgacgcgctc ggtgcgcggc tcgacaatgc cgccctgctg cacgcggccg
      961 gcgggcgcgt ggcgttctgc atgtccgcgt tcttccgcac ctacaacgcg ggcagcggcc
     1021 tgcggttcgg ggccggcctc gccgtggcca acggcttgcc gtggatcgag ggactgcgcg
     1081 cgatgaccag ctcgccggcg tcgattttcg gactcgacgg gcgctacggc cgggtacgtc
     1141 ccggtgccga cgccgacctg gtcgtctggg acggcgatcc gctcgagccg tcgacctccg
     1201 cgctgcaggt gtgggtccgc ggcgtcgaaa tgcccgtgga cgccacgcgc cagcacgcac
     1261 tcgccgaacg gtattcgccg ctgcggaaga gggagatgcc gccggcgtac aggcattagt
     1321 ggctagtggc tagtggctag tggcagagga gtacgccgac gcacttgctc cttcactagc
     1381 cactgaccac tgaccaccag ccacttcctc agtgggtttc ttccgtcccg aagtactgcc
     1441 gcggtggtac gccgaacgcg cgccggaaca tcgcggtgaa cgcgctcggg ctgttgtagc
     1501 cgacgtcgag cgccacggcc gtcacggact gtcccgtgga gaggcgtgac atcgcctcga
     1561 tcagccgcac gttctggcgc cacgtggcga acgtcacgcc ggtctcgctg cggaagatcc
     1621 gcgtgaacgt gcggcgcgac atgccggcgg cgacggccca gtcgtccagc acgtcgttct
     1681 gggccgggtc cttcaggatg gcgtgacaga tccgcacgag ctgcggatgg cgtggcatcg
     1741 ggacgtgcag cggcgtgtgc ggtgccgcgc ggatctcgtc gacgatcagc gtcatcacgc
     1801 gtccgtcgcg cgagtccacg tcgtactcga tcgggattcg cgtcgcctcg acgatcagct
     1861 cgcgcagcag cgtcgagacc tcgatcgtca tgcaggtgcg cggcaggccc ggggtcaccg
     1921 cgtcgtcgag gtagagcgtg cgcaacgaca cgtggccgcg tgtgcgcgcc tcgtgcacga
     1981 cgccgctcgg tacccacagt gcgcgctgcg gcggcaccac gaacgtcgca tcgcgcgtgg
     2041 tgcacacgag cacgccgctg atcgcataga cgagctggcc gcgcttgtgt gagtggggcg
     2101 ggtcgtacga gttcggcggg tactcgtcgg cgagcgccgc gaccggtcgc ggcacgtcct
     2161 ggtaatcgtc cccgcgcgtg ctccgtgccc ccatgcgtga tcaccgtggg cgctggccca
     2221 tccgagacga aatctgttcc ggcgccgatg gcgtcgggtg cgacccgagg ttatcagtac
     2281 cgcaacgccg caaacaaggc gcggtcggct gcgctggcgg ccggcgacga cacggacgca
     2341 acgcgcgtcg cgcaggcgcg cgcgcaggag agaccgagga tgagcagcaa cgctggcgaa
     2401 gcggccgtgg gccacggcgc aggcgacgac gcgatcgatc cggacatccg gcggttcgtc
     2461 gcgcgcgtga cggccgatta tgcacggctg acgggcggtc gcgagttgcc ggtcggcgag
     2521 atgcggagcg tcgccgagca ggtgcgcgag ccctggcgcg aaggcgggcc cgtcatggcc
     2581 agcgtgaccg agcgcgtcgc cgacacggag gccggaccgg tgcgactgcg gatctacgac
     2641 cccggggtcg cgaagcccgc cccggcgctc atctacatgc acggcggcgg ctgggtgctg
     2701 ttcagcctcg acacgcacga ccgcgtcatg cgggagtacg cggcgcgggc cggcgtcgtc
     2761 gtgatcggcg tcgactacgc gctctccccc gaggcgcgtt acccggccgc gctcaaccag
     2821 gtggtcgccg tcgtgcgctg gttgcagacg cgcggggcga aactcggcgt cgacgcgcgg
     2881 cggctcgcgc tcggcggcga ctcggccggc ggcaacctgt cgatctgcgc ggcgctcgcg
     2941 ttgcgcgacg cgggcgagcg tgatctcgta cgcggcctgc tgctgatcta cccggcgttc
     3001 gaccggcact gctccgcgga atcgctgcgt cgcttcggcg gccctggcgc cgtgctcacg
     3061 gccgaggaag tcgactactt ctgggaccat tacaccggcg gcgtcgacgt cagcaacgac
     3121 ccgcacgcca tgccgatgcg ggcctcactc gagggcctgc cgcccgtctg catgacgatc
     3181 cccgcctgcg acgtgctgac cgagcagagc cacgaaatgg ccgaccgtct gcgggcggcg
     3241 ggcgtcgacg tgtcgatgac ggtctatccg ggcgcgacgc actcgttcat cgaagccgtg
     3301 tcgatcgcgc cgctcgccga ccgggccatc gaggacggcg cccgctgggt gcggcggacg
     3361 ctcggtgccg gcgccccgac cggaccgcgc tgagccggcg cgccgcatga agcccgcgct
     3421 gcgcaagctg caccgctgga tcgcgctcac gctgggcgtg ctgtggctgt cgcaggcact
     3481 caccggcctc gtgatggtgt atcgctggga actcgacgac gccgcgctcg aggcgcctgc
     3541 cgtgccactc gatgccgcgg cgctcggcgc gcgcatcgag gccatcgagc gggatcccgc
     3601 gggcctcacg gtcacgagtc tctgggcgac cggcggggtc gacggacgct tcgacctcta
     3661 cgtcgacgac tcggccggcg acacggatat cgtgcgcgtc gacggccggg gccgggtgct
     3721 gcgcagccgg gggtcaggcg aaggcttcgt ccccactgcg gcctcgctgc accagacgct
     3781 gctcgccggc gaccgcggca agctcgtcgt cggcatcagc ggcgtcttcc tgctggtcac
     3841 gatcgtgatg gggctcgtgc tggcctggcc ggcgcgcgcc aggcagtggc gaccggcgct
     3901 gctgcccaaa ggcgcgcgcc cgggtgcggc gcgacgttat gcctggcacc gtgcgggtgg
     3961 gctgtggatc ggcatcccgg cgctggtgtc gctcggcgcc ggcgtgctgc tcacgttcga
     4021 gtcgacgctc gccggctggc tcggcaccga tgccacaccg ccggaactcg caggcatcga
     4081 ggcgccggcc ggcgcaccga tcgcgcccgc gcaggcgttc acgaccgcgc tcgcgcaatt
     4141 ccccggctcg agtctgagcg gggtgtcgtt tccgtcgcag gagcaaccgt ggttccgcat
     4201 ccgtgtgcgt cagccggagg agtggcggcg cgtctacggc acgagcgtcg tgtacgtcgc
     4261 ggcggcggac ggaacggtgc tgcgcaccga ggatgcgttg caggccccgg cggcgaccgc
     4321 gttcatgagc aacctctatc ccgtgcacac cggcgaggcg ctcggcgtcg tcggccgtgt
     4381 gttcgccctc gtggtcggcg cctggctgct ggccatgctc gtgctcggcg tcgggctctg
     4441 gctcgcccgc cggaggtgac gtgatgcggc aagacgatcc tgcgtgcagc catggtctcg
     4501 gccggctgtt cgcgcgtcgt tcgatcgagt cgctgcagtc cgacgcgcag cggtccggac
     4561 tgccgcggac gctcagcgcg ttcaacctgg tgctgctcgg gatcggttgc atcgtcggcg
     4621 ccggcatcta cgtgctgccg gggctcgcgg ccgcccactt cgcggggccg ggtgtcgtgc
     4681 tctcgttcct gctcgcgggg gccgcctgcg cgctcgcggc gctgtgctac gcggaactcg
     4741 cctccacgat gccggtgtcc ggctcggcct acgcctactg ctacgccgca atgggcgagg
     4801 tgttcgcgtg gtcgctcggc tgcctgctgc tgttcgagta cggcatcgcg gcggcactgc
     4861 tcgcggtcgg gttctcgggc tacctcggaa gcctgctccg ggacgtcggc atcacgatgc
     4921 ccgccgcact ctcgacaccg ctggtgcagg ccgagatcct gcctggcggc acgcggctgc
     4981 acgtcggcac ctcgttcaac ctcgtcgcgg cgctcgccat cgcgggcatc accgcggtgc
     5041 tcgtgcgcgg cgtgcaggcc tccgcgctgg tcaacaacgt gctggtgctg ctcaaggtca
     5101 cggtgctggg cggcctggtc gtggtgggcg ccgcggcggt cgacccggcg aactgggtgc
     5161 cgttcgtgcc acccaacgag ggcgggttcg cgttcggctg gcagggcgtc gtgcgggccg
     5221 cgtcgatcct gttcttcgcc tacatcggtt tcgagaccgt ctccaccgcg gcggccgagg
     5281 cgcgcaatcc gcagcgcgac ctgccgatcg gcatcctcgg ttcgctggcc gcctgcacgc
     5341 tgatctacgt ggtggtcgcg gccgtgctga cgggcatcgt gccgtatcgc gaactcggcg
     5401 tgccggaccc gatcgcgctc gccatcgatc gcctgcacat gccggtgctc gccagtctcg
     5461 tcaaggtcgg cgcgctcgcg ggcctcgctt cggtgctgct ggtcaacgcg ttcggccagt
     5521 cccgcgtcgc gttcgcgatg tcgcgggacg gcctgctgcc accgctgttc agccgactgg
     5581 gcgcgcgggt ccgcacgccg gtggccgggg tcgtgctgtt cgcgatcgtc tcggccctcg
     5641 gtgcggcgct gctgccgctc tcgctgctcg gtgacctgac cagcatcggc acggcgctcg
     5701 cgttcgcgac cgtctgcctg accgtgctgt ggctgcgcaa tgcgcggccg gacctgcacc
     5761 gtccgtttcg cgtgccgctc ggcggcttcc gcatcggcgg agtctggatc ggctgggtgc
     5821 cgctcggcgg catgatcctg tgcctcggca tggcggcgcc ggtcgcgatc gacgtgaccg
     5881 gccaggcgtt gcgcggccag gtgctgccgg tgctgatcat cgcgggctac gcggccgcgt
     5941 gcacgctcgc ctacttcggg tacgcggtca ggaactcgtc gctgcgacgc gtcggcgcga
     6001 cgatcgtcga gccaggggcg ccttaggccg accgggagtc tgtcgcgacg cggaggccgt
     6061 accatgcggg ccccggtcct cgacatcgat gcagggcact gacatgaagc actccagcaa
     6121 ccttccgccg ccacgcaatg aaccaatcca ggactacgcc ccgggttcga cacagcgcgc
     6181 acgcctcaag gccgcgatcg ccgacgtcga ggcggccgca agcgagattc cgggcttcgt
     6241 cggcgggcgg acggtccacg gcggcacacc agtccgggtg acttcaccgc accggcacga
     6301 gcgcgccgtc gcccagctgc accagctcgg ggcggacgag gtccgccagg ccatcgaggc
     6361 ggcggtcgag gcgcagcacg actgggccgc gctggcgttc gagcagcgtg ccgccgtgtt
     6421 cctgcgcgcg gccgacctga tcgccggccc ctggcgtgac cgcatgaacg ccgcgacgat
     6481 ggtcggccag agcaagacgg tcgtgcaggc cgagatcgac gctgcatgcg agctcgcgga
     6541 cttcctgcgc ttcaacgtgc actacgccga gcggctgctc actgagcagc cggagtcgag
     6601 cccgggcacc tggaaccgcg tcgactatcg ccccctggag ggcttcgtct acgcgatctc
     6661 gccgttcaac ttcaccgcca tcggcggcaa cctgtgcacg gcgccggcga tcctcggcaa
     6721 cacggtggtg tggaagcccg cgacgacggc gtcgctgtcc aactggcgca tcatggaagt
     6781 gctgcgtgag gcggggctgc ccgacggcgt gatcaacttc gtgcccggcg acgccgtgac
     6841 gatcacgcgc gaggtgctgg ccgatccgcg cctcgcgggc gtgcacttca ccggctcgac
     6901 ctccgtattc cagtcgatct ggcgcgaggt cggcgaacgc atcgaccgct atcggaccta
     6961 tcccaggctc gtcggcgaga ccggcggcaa gaatttcgtc gtcgcgcacc cgagcgccga
     7021 tgccgactcg ctcgcggtcg cgctggtgcg tggcgcgtac gagtaccagg ggcagaagtg
     7081 cagcgcggcc tcgcgtgcgt acgtgccggc gtcgctgtgg cccgagctgc gttaccgcct
     7141 gctgtccgag atctcgtcga tccgcgtcgg ggacccgaag gacttccgca acttcatggg
     7201 cgccgtgatt gaccgtcgcg cgtacgaccg gatccacggt tacctggaac gcgccgctgc
     7261 cgacacggac gtcgaggtgc tgaccggcgg caccggcgac acgtccgaag gctggttcat
     7321 cgagccgacg ctggtgcaga cgagcgatcc ggcgcaccgg ctgatgcgcg aggaactgtt
     7381 cggcccggtg ctggcggtgt acgtctacga cgactcggag tggcgcgagg tgctcgcgct
     7441 ggtcgaccgc acggccgagt acggcctgac gggcgccgtg ttcgcgcgcg accgcgccgc
     7501 gatcgtcgag gcggccacgg tgctgcgcaa caccgccggc aatttctacg tcaacgacaa
     7561 gccgacgggc gcggtcgtcg gccagcagcc gtttggcggc gcccgcgcca gcggcaccaa
     7621 cgacaaggcg ggctcgatcc tcaacctgtg gcgctgggtg tcgccccgca cgatcaagga
     7681 gaacttcgtg ccgccgcgcg acgttcgtta tccgtcgatg ggcgaggaat gagtcgcgca
     7741 cgtcgctggc ctcaattcga tagtttcagt ccctaacgcg aatgcgggcc acgcggggcg
     7801 cgcctaaaag tgcgaaaacg accgtcgggc ggcgcgacat cgcgaccgga cggcatacga
     7861 cgacggcgtc aaagtcactg cggaggtcac atgtctagcg gcactcacac gactcgcaac
     7921 ctgcgcctcg ccatcggttg cgtgctggcg ctcgcctcgg cggccgggtc cgcgcagcag
     7981 gcggcgtccg accaggaact cgaggaaatc atcgtcacgg gttcgcagat tcgcctgccc
     8041 gatccgttcg cgggcggcca ggtggccgaa ggcggccgcg ccggcatcct cggcaacctg
     8101 acgacgctcg agacgccgtt ctcgagcacg aactacaccg aggagctgat gcgcaaccag
     8161 caggcgaaga gcgtcgccga cgtcctgctg aacgacccga acgtgcgcgc ggcgcgcggc
     8221 ttcggcaact tccaggaact gttcatgatc cgcggctttc cggcctactc ggacgacatg
     8281 acgtacaacg gcatctacgg catcctgccg cgccagttcg tcgcgtccga gtacatggag
     8341 cgcgtcgaac tgttccgcgg tgccagcgcg ttcctgaacg gcgccgcgcc cgggggcagc
     8401 aacctcggcg gcacagtcaa cctcgtgccg aagcgtgcgc cggcagaagg cgtgagacgc
     8461 ctcaccgccg gctgggaaag cgattcggcc tactacctcg ccggggattt cggtcggcgg
     8521 ttcggtcccg acgaccagac cggcgtgcg
//
LOCUS       DASPFR010000155         3211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1144899, whole genome shotgun sequence.
ACCESSION   DASPFR010000155 DASPFR010000000
VERSION     DASPFR010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144899"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VD737_03925"
     CDS             complement(<1..401)
                     /locus_tag="VD737_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09737.1"
                     /translation="MLASLAVVALLRVTTSQADPGLQPYTARYHVSYRGLSGGEIESS
                     FRPGPRPGLWHYETRAYPSFLGRLAVSSQAHETSTMQVSADGVRPLSFSFDDGASDRK
                     KDIKLNYDWSAGKLTGVAEGKPIEFDLVPGV"
     gene            complement(514..1182)
                     /gene="purN"
                     /locus_tag="VD737_03930"
     CDS             complement(514..1182)
                     /gene="purN"
                     /locus_tag="VD737_03930"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HXW09738.1"
                     /translation="MTVASPAAVRVVVLVSGRGSNLKAIAAHADSGELPIEIVAVVSD
                     RAEAGALPWAASRGIATGVLSPADFPDRDAYGRALGELVESFRPGLVVLAGFMRILSD
                     GFVNRFEGRLLNIHPSLLPRHPGLHTHRRAIEAGDTVHGASVHFVTSELDGGPVVIQA
                     TVPVRAGDDESRLAARVLAQEHVIYPRCIGWFATGRLELKDGVPWLDGAPLAAPVAID
                     ATHD"
     gene            complement(1179..2219)
                     /gene="purM"
                     /locus_tag="VD737_03935"
     CDS             complement(1179..2219)
                     /gene="purM"
                     /locus_tag="VD737_03935"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008438191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HXW09739.1"
                     /translation="MAETPTTTTYRDAGVDIEAGDALVDRIKPAVKRSMRREVLGGIG
                     GFGALVEVPLDRYRRPVMVSGTDGVGTKLRLAIETGRHDTIGIDLVAMCANDVVVQGA
                     EPLFFLDYYATGKLDVSTAERVINGIVEGCLQAGCALVGGETAEMPGMYEGEDYDLAG
                     FCVGIVEKDAIIDGTRTRPGDAIIGLAASGPHSNGYSLIRRLLALSGAGPHTQLDGRP
                     LYDLLLEPTRIYVKSVLELLAQRVPVHGVAHITGGGPTGNIPRTLPHGMEAVIDARLW
                     PRQSVFDWLQRVGNVAREEMYRTFNCGLGMTLTVPATEADRALQLLRARGETAAVIGE
                     VRAGDREVVIVE"
     gene            2315..>3211
                     /locus_tag="VD737_03940"
     CDS             2315..>3211
                     /locus_tag="VD737_03940"
                     /inference="COORDINATES: protein motif:HMM:NF021367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HXW09740.1"
                     /translation="MTSKRSHVTSPARARRLARVLLAACTTCMVAWAPSNVAFAAASE
                     TPYQAVVPLQGTTEGDRAAAIGEALKVAVVRASGRTDAATAPRVVAAAADPSSYVQQY
                     STTPDRMLRVGFDASAMDQLLQQAGLPWWPAERPPVTVYLFTTSVAGGARALTGADQA
                     AERLEVERAAQARGVPVAWPAGAIDPASARARLGADRAVLLGLGAAGNYEWVYAHAGQ
                     TLRTQGGVAQAVDVVADALAARYAPASTRSSSVVLLRIGGLDGVRDYAALTEYLDGLS
                     LVREVTVKQLQRDAVQFELEVRG"
BASE COUNT          474 a         1137 c         1122 g          478 t
ORIGIN      
        1 tgcacgccgg ggaccaggtc gaactcgatc ggcttgccct ccgcaacgcc ggtcagcttg
       61 ccggcagacc agtcgtagtt gagcttgatg tctttcttgc ggtcgctggc gccgtcgtcg
      121 aacgagaacg acagcggacg cacgccgtcg gcggacacct gcatcgtgct cgtctcgtgc
      181 gcctgcgagc tgaccgcgag tcgccccagg aaactcggat acgcccgtgt ttcgtagtgc
      241 cacagccccg gccgcgggcc cgggcgaaac gaggactcga tctcgccgcc gctcaggcca
      301 cggtagctca cgtggtagcg cgcggtgtac ggctgcagtc cgggatctgc ctgcgacgtc
      361 gtcaccctga gcagcgccac gacggcgagc gacgccaaca tgccggcacg taagaacgga
      421 cgccgcgcat caggtgcatc gtgatcgggg gtgccggtgc ctggtgtcat cgagctgaat
      481 ctcctgcgcg tcggccgggc gccgatcgcc cggttagtca tgcgtcgcat cgatcgcgac
      541 cggcgcggcc agcggcgcgc cgtcgagcca gggcacgcca tccttgagtt cgagtcgccc
      601 cgttgcgaac cacccgatgc accgcggata aatcacgtgc tcctgcgcca ggacacgcgc
      661 cgcaagacgg ctctcgtcgt cgccggcccg caccggcacc gtcgcctgta tgaccaccgg
      721 tccgccatcg agttcggacg tgacgaagtg aacactggcg ccgtgcacgg tgtcgccggc
      781 ctcgatcgca cgccggtgcg tgtgcaggcc ggggtggcgc ggcagcagcg acggatggat
      841 gttcaacagg cggccttcga atcggttgac gaagccgtcg ctcaggatgc gcatgaagcc
      901 ggccagcacc accagccccg gccgaaaact ctccacgagt tcgcccagcg cgcggccgta
      961 cgcgtcccga tccggaaagt ccgccggcga gagcacgccg gtcgcgatgc cgcgcgaagc
     1021 tgcccaaggc agggcgcctg cctctgcgcg atcgctgacg accgccacga tctcgatcgg
     1081 cagttcgccg gagtcggcgt gcgcggcgat cgccttcagg ttgctgccgc ggcccgacac
     1141 cagcaccacg acgcgcacgg cggcaggcga cgcgacggtc attcgacgat gacgacttcg
     1201 cggtctcccg cgcgcacctc gccgatcacc gccgcagtct caccgcgcgc gcgcagcaac
     1261 tgcagggcgc ggtcggcctc cgtcgccggc acggtgagcg tcatgcccag gccgcagttg
     1321 aacgtgcggt acatttcctc gcgcgcgacg ttgccgacgc gctgcagcca gtcgaacacc
     1381 gactgccgcg gccagagccg cgcatcgatt acggcttcca tgccgtgcgg cagcgtccgc
     1441 gggatgttgc cggtggggcc gccgccggtg atgtgcgcga cgccgtggac cggcacccgt
     1501 tgcgcgagca gctcgagcac ggacttcaca tagatccgcg tcggctcgag cagcaggtcg
     1561 tacagcggtc gaccgtcgag ctgcgtgtgc ggacccgcgc cgctcagcgc gagcagccgg
     1621 cggatcagcg aataaccgtt cgagtgcggt ccggaggcgg cgagaccgat gatggcgtcg
     1681 ccgggccgcg tgcgcgtgcc gtcgatgatg gcatccttct cgacgatgcc gacgcagaaa
     1741 ccggccaggt cgtagtcctc gccctcgtac atgccgggca tctccgcggt ctcgccaccg
     1801 accagcgcgc agccggcctg caggcacccc tcgacgatgc cgttgatgac gcgctcggcg
     1861 gtggacacgt cgagcttgcc ggtcgcgtaa tagtcgagga agaacagcgg ttcggcgccc
     1921 tgcacgacca cgtcattggc gcacatcgcc accaggtcga tgccgatcgt gtcgtgacga
     1981 cccgtctcga tcgcgaggcg cagcttggtg cccacgccgt cggtgccgga gaccatcacg
     2041 gggcgtcgat atcggtcgag cggcacctcc acgagcgcgc cgaaaccgcc gatgccgccg
     2101 agcacctcgc ggcgcatcga acgcttgacc gcgggcttga tgcggtcgac gagcgcgtcg
     2161 ccggcctcga tgtcgacgcc tgcgtcgcga taggtcgttg tggtcggagt ctcggccatc
     2221 gtggatcgtc tctcgggagc gggaggcccc aaggcgcggc tcgaggctgt ggtattttac
     2281 acggcggccg cagcgtcgga ctcctgatcg atcaatgact tccaagcgct cccacgtcac
     2341 gagccccgcg cgtgcccgcc gtctcgcacg tgtcctgctc gccgcctgca cgacgtgcat
     2401 ggtggcgtgg gcaccgtcga acgttgcctt tgccgccgcc tcggagaccc cttaccaggc
     2461 cgtcgtgccg ctccagggga ccacggaggg cgaccgcgcg gcggcgatcg gcgaagccct
     2521 caaggtggcg gtcgtgcgcg cctcggggcg caccgacgct gcaaccgcac cacgggtggt
     2581 cgcggcggcg gccgatccgt cgagctacgt acagcagtac tccacgaccc ccgaccgcat
     2641 gctcagggtg gggttcgacg cgagcgcgat ggatcagttg ctgcagcagg caggcttgcc
     2701 ctggtggccg gcggaacgcc ccccggtgac cgtctacctg ttcacgacat cggtcgccgg
     2761 cggtgcgcgc gcgctgaccg gtgcagacca ggcagccgaa cgcctcgaag tcgaacgggc
     2821 cgcgcaggcg cggggcgtcc ccgtcgcgtg gcccgccggc gcgatcgatc cggcctcggc
     2881 gcgcgcccgg ctcggtgcgg accgcgcggt gctgctcggg ctcggcgccg ccggcaatta
     2941 cgaatgggtc tacgcgcacg ccgggcagac gctgcgcacg cagggcggtg tcgcgcaggc
     3001 ggtcgacgtg gtcgccgacg cgctggcggc gcgctacgcg cccgcatcga cgcgttcgag
     3061 cagcgtcgtg ctgctgcgga tcggtggcct cgacggtgtc cgcgattatg ccgccctgac
     3121 cgaatacctc gacgggttga gcctcgttcg cgaggtgacc gtgaagcagc tgcagcggga
     3181 cgcggttcag ttcgagctcg aggtgcgcgg g
//
LOCUS       DASPFR010000156         4799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1022832, whole genome shotgun sequence.
ACCESSION   DASPFR010000156 DASPFR010000000
VERSION     DASPFR010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022832"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..978
                     /locus_tag="VD737_03945"
     CDS             40..978
                     /locus_tag="VD737_03945"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXW09741.1"
                     /translation="MTFHPDVLNYFASVASEFPPPGTEPTAQASRALHQRLARRLGVG
                     RALQTVRTFTVPGPTGEIACRLYSDRPPGSLPLLIFLHGGGWIGGDLDTHDVFCRELA
                     FGADCGVIAVDYRLAPENKFPGGYEDCLAACTYILAHAAELGIDPARIAIGGDSAGGN
                     LAAAVVQALRQAEGKQPCFQLLIYPFTDFRMATPAFTEMQVPGFSAREAAWCVDQYLS
                     SISEARDLRASPALAGSLSGLPPTFVVTAEFDVLRDDGEAYALALAKAGVPVQVRRYL
                     GVPHGFLAMPPDLEVTASAIGDVCKALRAAFEEKAA"
     gene            975..1916
                     /locus_tag="VD737_03950"
     CDS             975..1916
                     /locus_tag="VD737_03950"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXW09742.1"
                     /translation="MTTATARPNFGWKLRLVIAVQTWLVRLRIMPDADAYARWPVERR
                     KALKPAPWMTYPPPASVQTKRETLATADGPLEMKLFSSGSVQAPSVLFIHGGGWVVGG
                     VDSLDYLCANLCDRLNVGVAAVDYRLAPDHRFPAGLEDCYSALTWLATQAQRPILVVG
                     DSAGGNLAAALCLLARDRGGPVIAHQTLIYPALDACMESPSMALEKTGMTRADCDAIV
                     RHYLGDHDPRDPLVSPIHATDLGGLPPAFIVTADHDLLRDDGARYAECLRTAGVEVRY
                     VNYPDMPHGFLSQPRLCAGAPAALADIIEEVRTRLVH"
     gene            1971..3668
                     /locus_tag="VD737_03955"
     CDS             1971..3668
                     /locus_tag="VD737_03955"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HXW09743.1"
                     /translation="METALSPLDFMRRSRKLYPDREAVVDGELRMSYAEFFARCDRWS
                     AALARLGVGKGDRVATLAPNTHQHLEQFYAVPQLGAVLVPLNYRLVAEDFVYLANHSG
                     TKVLCVHGEYLDVIDGVRDRMTSVEHFVSLEGRKPGWLEYEPLLAEGDGAFVRPEIDE
                     LDPLTINYTSGTTSKPKGVVITHRNAWVNAVGTLVHLPMTPADRYLWTLPMFHANGWT
                     FTWIVSAVGGTHVCLRQVDASAVYRTVRAERISTLCAAPTVLIGIANGPAAERRDLPG
                     GVRVITAGAPPAAATIERMESELGWTVTQVYGLTETSPLISICEPRPEHASLSVAERA
                     RIKARQGVDLVTSGDLRVVDETMNDVPHDGQTLGEIVARGNVVMQGYYNDPEATATAF
                     RGGYFHTGDAAVVHPDGYVEIRDRYKDVIISGGENISSIEVEGALLAHPDVLEVAVVG
                     MSHEKWGESPHAFVVLKTGKSLTSDDLRQYARGVLAHFKVPTVFTFVDSLPKTATGKI
                     QKYVLRGGKAAIARQERRVSAEASRAGVGLARRRRDASCTQYLGIRSPGCARKCLIR"
     gene            3880..>4799
                     /locus_tag="VD737_03960"
     CDS             3880..>4799
                     /locus_tag="VD737_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09744.1"
                     /translation="MLKWPSLAGAFACLFLAANAWAQSASVKAASSAEVGSKLQVHWT
                     GPAAQGDFVSIDAAGAPDHVYGPYAYPSHGNPLTLLVPTTSGNYEIRYHVGASGYRVL
                     ARTPLAVTDVAATLEAASTVEAGGSVRVSWKGPNNQGDFISIDPVGAPDRTYGNYAYP
                     AKGNPVDVRAPDQPGDYLLRYHLANSYRVIGSVPLKVGGVAATLEFPANARAGSTLSV
                     KWTGPGNKGDFISIDAVGAADRTYGNYAYPEKGNSVEIRVPDDPAEYVVRYHLASSNR
                     VIGSAPLKVEPVTASLTAPAKVPARSVFEV"
BASE COUNT          801 a         1630 c         1584 g          784 t
ORIGIN      
        1 ccatcgcaca ctgcagacgg acgcataggg gctcggacga tgacgtttca cccggatgtc
       61 ctcaactatt tcgcaagcgt cgccagcgag tttcccccac ccggaaccga accgacggcg
      121 caggcctcgc gggccctgca ccagcgattg gcccggcgac tcggtgtcgg ccgtgccctc
      181 cagaccgtcc gcaccttcac ggtcccgggt ccgaccggcg aaatcgcgtg ccggttgtat
      241 tcggatcgcc cgccaggatc gctgccgctg ctgatcttcc tgcacggggg cggctggatc
      301 ggtggcgacc tcgacacgca cgacgtcttc tgtcgcgagc tcgctttcgg cgcggactgc
      361 ggcgtcatcg cggtggacta tcgactcgct ccggagaaca agtttccggg cggctacgag
      421 gactgcctcg ccgcttgcac gtatatcctc gcgcacgccg ccgaactcgg catcgatccg
      481 gcacgcatcg cgatcggagg cgacagtgcc ggaggcaacc tcgcggccgc ggtcgtgcag
      541 gcgcttcgtc aggcagaggg caagcagccc tgctttcagc ttctgatcta cccgttcacc
      601 gacttccgga tggcgacccc tgccttcacc gagatgcagg tgcccgggtt ttcggcccgc
      661 gaggccgcct ggtgcgtgga ccagtatctt tcgtcgatca gcgaggctcg cgaccttcgc
      721 gcgtccccgg ccctggctgg gagcctgtcg ggcctgccac ccacgttcgt ggtgacggcc
      781 gagttcgacg tgctgcgcga cgacggcgaa gcctacgcgc tcgcactcgc gaaggcgggc
      841 gtgccggtgc aggtgcgtcg ctatctcggc gtgccccacg gattcctggc gatgccgccg
      901 gacctcgagg tcacggccag cgcgatcggg gacgtctgca aggccctgcg tgcagccttc
      961 gaggagaaag ccgcatgacg acggcaacgg cacgaccgaa cttcggctgg aagctgcggc
     1021 tggtcatcgc ggtgcagacc tggctcgtgc gtctccggat catgccggac gccgatgcct
     1081 acgcccgctg gcccgtcgaa cggcgcaagg cgctgaagcc cgcgccgtgg atgacctacc
     1141 cgccgccggc atcggtgcag acgaagcgag agaccctcgc gaccgcagac ggcccgctgg
     1201 aaatgaagct gttcagcagc ggctcggtgc aggcaccgtc ggtcctgttc atccacggcg
     1261 gtggctgggt cgtcggcggt gtcgattcgc tcgactacct ctgcgccaac ctctgtgatc
     1321 gattgaatgt cggcgtcgcc gcggtcgact accggctcgc accggatcac cgtttccctg
     1381 cgggcctcga ggactgctac tcggcgctga cgtggctggc cacgcaggcg caacggccga
     1441 tcctggtggt cggtgacagc gccgggggca acctggccgc ggccttgtgc ctgctcgctc
     1501 gtgaccgagg cgggccggtc atcgcgcacc agacgctgat ctatccggcg ctcgatgcct
     1561 gcatggaatc gccgtcgatg gcgctcgaga agacgggcat gactcgcgcc gactgcgacg
     1621 ccatcgtgcg gcactacctg ggcgatcacg accctcgcga tccgctcgtg tcgcccatcc
     1681 acgccacgga cctgggcggc ctgccgcccg cgttcatcgt cacggcggat cacgacctgc
     1741 tgcgcgacga cggggcgcgc tacgcggaat gcctgcggac ggccggggtc gaggtccgat
     1801 acgtcaacta ccccgacatg cctcacggat tcctgtccca gcccaggctc tgcgcgggcg
     1861 caccggccgc ccttgcggat atcatcgaag aagtccgcac gcgactcgtg cactgaacga
     1921 ggcgcggacc tgcggtcttg ttgcaatcta ccggaacacg gtgaaaggcc atggaaacag
     1981 cactctcacc gctcgacttc atgcgccggt cgcggaagct gtatcccgat cgcgaggccg
     2041 tggtcgacgg cgagctccgg atgtcgtacg cggagttttt cgcccgctgc gaccgctggt
     2101 cggccgcgtt ggcccggctc ggagtcggaa agggtgaccg cgtcgcgacg ctcgctccga
     2161 acacccacca gcacctcgag cagttctacg cggtgcccca gctcggggcg gtcctcgtgc
     2221 cgctgaacta ccggctggtg gccgaggatt tcgtctacct tgccaatcac agcggcacga
     2281 aggtgctgtg cgttcacggc gagtacctgg acgtcatcga cggcgtccgg gaccgcatga
     2341 ccagcgtcga gcacttcgtg tcgctcgaag gtcgcaagcc gggatggctc gagtacgagc
     2401 cactgctggc cgaaggcgac ggagccttcg ttcgcccgga gatcgacgaa ctcgatccgt
     2461 tgacgatcaa ctacacgagc ggcacgactt cgaaaccgaa gggcgtggtg atcacgcatc
     2521 gcaacgcctg ggtgaacgcg gtcgggacgc tggtgcacct gccgatgacg ccggccgacc
     2581 gctacctgtg gacgctgccg atgttccacg caaatggctg gacgtttacg tggatcgtga
     2641 gcgccgtggg cggcacgcac gtctgcctgc ggcaggtcga tgcctctgcc gtgtaccgca
     2701 cggtgcgcgc cgaacgcatc agcacgctct gcgcggcacc gacggtgctc atcggcatcg
     2761 ccaacggccc cgcggccgag cgccgggacc tgcccggcgg cgtgcgggtg atcacggccg
     2821 gcgcgccgcc cgcggccgcg accatcgagc gcatggagtc cgagttgggc tggacggtga
     2881 cgcaggtcta cggactcacc gagacttccc cactgatctc gatctgcgag ccgcgccccg
     2941 agcacgcaag tctctcggtg gctgaacgcg cgcgcatcaa ggcccgccag ggcgtcgatc
     3001 tcgtgacctc cggggacctg cgcgtcgtcg acgagaccat gaacgacgtg ccccacgacg
     3061 ggcagacgct cggcgagatc gtcgcgcgcg gaaacgtcgt catgcagggt tactacaacg
     3121 atcccgaggc gaccgcgacg gccttccgcg gcggctactt ccacaccggc gacgccgccg
     3181 tcgtgcatcc cgacggctac gtcgagatcc gcgaccgcta caaggacgtg atcatcagcg
     3241 gcggcgagaa tatctcgtcg atcgaggtcg agggcgcgct gctggcgcat cccgacgtgc
     3301 tcgaggtcgc ggtcgtcggc atgtcgcacg agaagtgggg cgagtcaccg cacgcgttcg
     3361 tcgtgctgaa gacaggaaag agcctgacgt cggacgacct gcggcagtac gcgagaggcg
     3421 tcctcgcgca cttcaaggtg ccgacggtgt tcacgttcgt cgacagcctg cccaagaccg
     3481 cgactggaaa gatccagaag tacgtgctcc gcggcggcaa ggcggcgatc gcgcggcagg
     3541 agcggcgcgt ttcggccgag gcatcacgcg caggcgttgg ccttgcacgt cggcggcgcg
     3601 atgcttcgtg tacccagtat ctcgggatcc ggtcgccggg atgcgcgcgt aagtgcttga
     3661 ttagatagtg gacccgtgta ggactcgaac ctacgaccta ccgattaaga gagagcggca
     3721 cacccctgtt tcaaaggggg ttttcccgac gatcgtgtac cgccgctcaa cggcaggaga
     3781 cccttaggtc tagttcgcaa attaacgtga acccctcgca gcggactgcc gaccgacgca
     3841 ggcactagaa ttgcgcagat ctcgccgagg tgcgctctca tgctcaaatg gccatccctc
     3901 gccggcgcgt tcgcatgcct gttcctcgcg gccaacgcct gggcacagtc agcgagcgtc
     3961 aaggccgcat cgagcgccga agtcggcagc aagttgcagg tgcactggac cggcccggca
     4021 gcacagggcg acttcgtcag catcgacgcc gccggggcac ccgaccacgt ctacggaccg
     4081 tatgcctatc cgtcgcacgg caacccgctc accctgctgg tgcctaccac gagcggcaac
     4141 tacgagatcc gttatcacgt cggtgcgagc ggatatcgcg tactggcgag gacgccactc
     4201 gccgtaaccg acgtcgcggc gacgctcgag gcggcgtcga cggtcgaggc cggtggttcg
     4261 gtgcgagtca gctggaaagg gcccaacaac cagggcgatt tcatctcgat cgatcccgtc
     4321 ggtgcgcccg accgaacgta cggcaactac gcttacccgg cgaagggtaa tccggtggac
     4381 gtgcgcgcac cggatcagcc cggcgattat ctgttgcgct atcacctcgc gaacagttac
     4441 cgcgtgatcg gcagcgtgcc gctcaaggtc ggcggtgtcg cggcaactct cgagtttccg
     4501 gcgaatgcgc gtgccggcag cactctgtcc gtcaagtgga ccgggccagg caacaaaggc
     4561 gacttcatca gtatcgacgc tgtcggggcg gccgatcgca cctatggcaa ctacgcgtat
     4621 cccgagaaag gcaattccgt cgagatccgc gtgcccgacg atccagccga gtacgtggtg
     4681 aggtatcacc tcgcgtcgag caacagggtc atcggcagcg cgcccctcaa ggtcgagccc
     4741 gtcactgcga gcctgacggc ccctgcgaaa gtccctgcgc gcagcgtgtt cgaggtgag
//
LOCUS       DASPFR010000157         3927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1388442, whole genome shotgun sequence.
ACCESSION   DASPFR010000157 DASPFR010000000
VERSION     DASPFR010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388442"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(384..1691)
                     /gene="clpX"
                     /locus_tag="VD737_03965"
     CDS             complement(384..1691)
                     /gene="clpX"
                     /locus_tag="VD737_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005315369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HXW09745.1"
                     /translation="MADDRSKHDDGKLLYCSFCGKSQHEVRKLIAGPSVFVCDECVEL
                     CNDIIREELEEKAEKTREKLPKPQEIKRVLDEYVIGQDRAKKVLSVAVYNHYKRLQTK
                     SSERTRDDIELAKSNILLIGPTGSGKTLLAETLARLLNVPFTIADATTLTEAGYVGED
                     VENIIQKLLQKCDYDVEKAQTGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLI
                     EGTIASVPPQGGRKHPQQEFLQVDTGNILFVCGGAFAGLEKIIMNRTHKGGIGFQAEV
                     REQNARQHGSDLFHDVEPEDLIKYGLIPEFVGRLPVVATLDELDEAALMQILTEPKNA
                     LTKQYRKLFDMEGVDLEFREESLRAVARKAMQRKTGARGLRTILENTLLDIMYDLPSM
                     RNVQKVVIDEDVIQGDSKPFLVYGQDDARLAAVEERRTGSGSA"
     gene            complement(1818..2450)
                     /gene="clpP"
                     /locus_tag="VD737_03970"
     CDS             complement(1818..2450)
                     /gene="clpP"
                     /locus_tag="VD737_03970"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HXW09746.1"
                     /translation="MSSIEPRALNLVPMVVEQTARGERAYDIYSRLLKERVVFCVGPV
                     EDYMANLIVAQMLFLESENPEKDIAIYINSPGGSVSAGLAIYDTMQFIRPDINTICVG
                     QAASMGAVLLAGGTKGKRFCLPHSRVMIHQPLGGFQGQATDIEIHAREILDARHRLNA
                     ILAKHTGQTIDRIKQDTDRDNFMTGEQATAYGLIDAVLQQRSEGGVATRT"
     gene            complement(2458..3753)
                     /gene="tig"
                     /locus_tag="VD737_03975"
     CDS             complement(2458..3753)
                     /gene="tig"
                     /locus_tag="VD737_03975"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HXW09747.1"
                     /translation="MQVSVESTGSLERRMEVSVPKEDIEQAVDERLKKVSRTARLKGF
                     RPGKAPIKVIRQQFGAQVRQEVITDLVQSSFAQAVTEQKLTPAAGPRIEPISSAPGED
                     LKYRATFEVYPEVALKQLDGLAVTRPVAEVSEADIEAMVQNLREQRPQFEPVERASRE
                     GDRVTMDFEGAIDGRAFEGSAGTDVAVVLGAGRMLRDFETGITGATAGEHKTVAVRYP
                     DEYHNKDLAGRTAEFSVHVKKVEEKQLPPLDDAFCREYGVNEGGVEQLLSEVADNMRR
                     ELAENVRARLKQQLFDRLLEANPLDVPKALVDQQVREMQVDTARRMGAKDASQVPPPD
                     AFVEPARRRVALGLLVGELVRTQGLQVDRGRVDARLAELVATYPDPDAILKAYRQNPD
                     AMRQVESMVLEDQVVDYLLERAKVTEQPSSFKELMNFGA"
BASE COUNT          568 a         1314 c         1283 g          762 t
ORIGIN      
        1 gggatgacca tgtgcggata gacgacgacg tcgcgcagcg gcagcaccgg catcggctcg
       61 gtgcgcgcgg cggcagtctt cgtcgacggg gcgttcgatt ctggggcgtt ggtcacgtgt
      121 cggtctccag accgtctgca ccctctcccg ccgtgcggga cagggttcgg acaagggttc
      181 gatccctcgc gcgcctgcgc ggcgccggga acgaagtggg ggcctgcgcg gccgggaaaa
      241 tattatcgcg ccagagcgac gcgaggggga aaaagaagtg gctagtggtc agtggtcagt
      301 ggccagtccg gattcggaac gcggcggagc cgcctcttca ccagccacta gccactagcc
      361 actgaccacc agccactagc cgttcaggcg cttccgctcc ccgtccgccg ctcttccacc
      421 gcggcgaggc gcgcgtcgtc ctggccgtag acgaggaagg gcttgctgtc gccctggatc
      481 acgtcctcgt cgatgacgac cttctgcacg ttccgcatgc tcggcaggtc gtacatgatg
      541 tcgagcagcg tgttctcgag gatcgtgcgc aggccgcgcg cgccggtctt gcgctgcatc
      601 gccttgcgcg cgaccgcgcg cagcgactcc tcgcggaact cgaggtcgac gccttccatg
      661 tcgaagagct tgcggtactg cttcgtcagg gcgttcttgg gctcggtcag gatctgcatc
      721 aacgccgcct cgtcgagctc gtcgagcgtc gcgacgaccg gcaggcgtcc gacgaattcc
      781 gggatcaggc cgtacttgat caggtcctcg ggctcgacgt cgtggaacag gtcgctgccg
      841 tgctggcgcg cgttctgctc gcggacttcg gcctggaagc cgatgccgcc cttgtgggtg
      901 cggttcatga tgatcttttc gagcccggcg aacgcgccgc cgcacacgaa caggatgttg
      961 cccgtgtcga cctgcaggaa ctcctgctgc gggtgcttgc ggccgccctg cggtggcacc
     1021 gaggcgatcg tgccctcgat gagcttgagc agcgcctgct gcacgccttc gccggagacg
     1081 tcgcgcgtga tcgacgggtt gtcgctcttg cgcgagatct tgtcgatctc gtcgatgtag
     1141 acgatgccgg tctgcgcctt ctcgacgtcg tagtcgcact tctgcagcag tttctggatg
     1201 atgttctcga cgtcttcgcc gacatagccc gcttcggtga gcgtcgtcgc gtcggcgatc
     1261 gtgaacggca cgttcagcag ccgcgcgagc gtctcggcca gcagcgtctt gccggagccg
     1321 gtcgggccga tcagcaggat gttgctcttg gcgagctcga tgtcgtcgcg cgtgcgctcc
     1381 gatgacttcg tctgcagccg cttgtagtgg ttgtagaccg cgacggacag caccttcttg
     1441 gcgcggtcct gcccgatcac gtattcgtcg aggacgcgct tgatctcctg cggcttcggc
     1501 agcttctcgc gcgtcttctc ggccttttcc tcgagctcct cgcggatgat gtcgttgcac
     1561 agctcgacgc attcgtcgca gacgaacacc gacgggcctg cgatgagctt gcggacctcg
     1621 tgctggctct tgccgcagaa ggaacagtag agaagcttgc cgtcgtcgtg cttggagcga
     1681 tcgtccgcca tgtgcctcga accctgcgcc aggaggccaa aattcctggc tcgatggctt
     1741 ataccacgcg agccccggtc cgcaaaaaac ctttctgctg cagtgacttg aagctcagcc
     1801 cccggttccg ggcgccgtca cgtgcgcgtc gccacgccac cctcgctccg ctgctgcagc
     1861 accgcgtcga tcaggccgta tgcggtcgcc tgctcgccgg tcatgaaatt gtcacggtcc
     1921 gtgtcctgct tgatccggtc gatcgtctgg ccggtgtgct tggccaggat cgcgttcagc
     1981 cggtggcggg cgtcgaggat ctcgcgcgcg tggatctcga tgtccgtcgc ctggccctgg
     2041 aagccgccca gcggctggtg aatcatgacc cgcgagtgag gcaggcagaa ccgcttgccc
     2101 ttcgtgccgc ccgcgagcag caccgcaccc atgctcgccg cctgccccac gcagatcgtg
     2161 ttgatgtccg gccggatgaa ctgcatcgtg tcgtagatcg cgagtcccgc gctcaccgag
     2221 ccgcccggcg agttgatgta gatcgcgatg tccttctccg ggttctccga ctcgaggaag
     2281 agcatctgcg cgacgatcag gttcgccatg tagtcctcga ccgggccgac gcagaacacc
     2341 acccgctcct tgagcaggcg cgagtagatg tcgtaggcgc gctcgccacg ggccgtctgt
     2401 tcgacgacca tcgggacgag gttcaaggca cgcggttcga tggatgacat ggtgtgatca
     2461 ggctccgaag ttcatcagtt ccttgaaact cgacggctgc tcggtgactt tcgcccgttc
     2521 gagcaggtag tccaccacct gatcctccaa caccatgctt tcgacctgtc gcattgcatc
     2581 agggttctgg cggtaggcct tcaggattgc atccggatcg gggtaagtcg ccacgagctc
     2641 agcgaggcgc gcatccacgc gcccccggtc gacctgcagg ccctgggtcc tgacgagttc
     2701 gccgaccagc agcccgagcg cgacccggcg ccgcgccggc tcgacgaatg catcgggcgg
     2761 cgggacctgc gacgcgtcct tcgcgcccat gcgcctcgcc gtatccacct gcatctcgcg
     2821 cacctgctgg tccacgagcg ccttcggcac gtcgaggggg ttcgcctcga gcagccggtc
     2881 gaacagctgc tgcttgaggc gggctcgaac gttctcggcg agctcgcgac gcatgttgtc
     2941 ggcaacctcc gataacaact gctcgacccc gccttcgttg acgccgtatt cccggcagaa
     3001 ggcgtcgtcg agcggcggca gctgcttctc ctccaccttc ttgacgtgca ccgagaactc
     3061 cgccgtgcgg ccggcgagat ccttgttgtg gtactcgtcc ggatagcgca cggcgacggt
     3121 cttgtgttcg ccggctgtcg cgcccgtgat ccccgtctcg aaatcgcgga gcatgcgccc
     3181 ggcgcccagc accacggcta cgtccgtgcc ggcgctgccc tcgaacgccc ggccgtcgat
     3241 cgcaccctcg aagtccatgg tcacgcggtc gccctcgcga ctcgcgcgct cgaccggctc
     3301 gaactgcggg cgttgctcgc gcaggttctg gaccatggcc tcgatgtcgg cttcgctcac
     3361 ctcggcgacc gggcgcgtga cggccaggcc gtccaactgc ttgagcgcca cctcggggta
     3421 cacctcgaag gtcgcgcggt acttgaggtc ctcgcccggg gcgctcgaaa tcggctcgat
     3481 gcgggggccg gcggccggcg tgagcttctg ctcggtcacg gcctgcgcga agctcgactg
     3541 cacgaggtcg gtgatgactt cctgccgcac ctgcgcgccg aactgctggc ggatcacctt
     3601 gatcggcgcc ttcccggggc ggaaaccctt gagccgggcc gtacgactga ccttcttgag
     3661 gcgctcgtcg accgcctgct cgatgtcctc cttggggaca ctgacttcca tccggcgctc
     3721 gaggctgccg gtcgactcaa ccgaaacttg catggttcct tcgatcctca ggcgtctgcg
     3781 ggccgaaccc ggaaagtagc cgcgtattgt gccacggagg gagtggctgg tcagtggcca
     3841 gtggccagtg gccagtggcc agtggctagt ggctagtggc tagtggctag tggctagtgg
     3901 ctggcaagga cctggaagga gggcccc
//
LOCUS       DASPFR010000158         6321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_656090, whole genome shotgun sequence.
ACCESSION   DASPFR010000158 DASPFR010000000
VERSION     DASPFR010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656090"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..2180
                     /locus_tag="VD737_03980"
     CDS             273..2180
                     /locus_tag="VD737_03980"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HXW09748.1"
                     /translation="MSTPPLIVDATVQFLRAHLPFSRMARKDLEFVAERARLGYFPVG
                     TVIVDPASGIAEFLYIIQRGHVRVRNPNAAVDDEVRGAGECFPVIALSAASAGSRTFE
                     ATEDVFCLLLPRADFDELRRRSAEFAQFCTEALATVVQHSLGQLRHHFSQRAADQQTL
                     LEPIKALVRRDPVYCLVDTPVRAALERMRQEGVRTIAVVDTGRRPVGVFTLIDLMERV
                     VLPGVTLSTPVSEVMSACPVVVDELTTAQDGLGLMASRGFHQLLVVQGGELTGIVTER
                     DLFGLQRVTMRNVTQSIRLARDVPALRRCVDDIQSLTENLLAQGAAAEPLTHTITALN
                     DALTQRLFELLLPQFPRVAPLSWCWLAVGSEGRREQTVATDQDNAIVFRATARDVEPA
                     REALLEFARAANHALAELGFPLCVGNIMAGNPDCCLTLEEWRARFASWMREPTPTALL
                     GANIFFDFRALSGDGTLAATLRQWLDATSGENKLFLRMLVANALQAEPPLGWIRTFRT
                     DAGETIDLKTHGTRIFVDAARAFALALGIPETNTAQRIRVAGRRLARDERDITATVDA
                     YQFLQLLRLRVQRGGLETAAAVEAPADKGPRAPNRLDPYALNELDQRMLREAFRQARA
                     LQTHLDQSVGH"
     gene            2183..2905
                     /locus_tag="VD737_03985"
     CDS             2183..2905
                     /locus_tag="VD737_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HXW09749.1"
                     /translation="MLLPSTFGEVWRSRRIRRSLPAELQARLGRVHRSFLDRPGLDQP
                     LALARFIALDLETTGPRMLTDRIISIGAVAVTDGTVRHDDAFEALLRQERSSAVDNIL
                     VHQIGGQAQLGGTDPAEALVRCLEFVGDSRIVAFRAEFDQTVFRREARLRLGVHAWPR
                     FIDLAALLSALFPGTANDTLDEWVGHFGLPPIGRHHAIADAYATARLLMVVLQAAARA
                     GIETSRDLRDAEQAQRWLGRRR"
     gene            3030..4277
                     /locus_tag="VD737_03990"
     CDS             3030..4277
                     /locus_tag="VD737_03990"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HXW09750.1"
                     /translation="MTAVPVMQPNPRLLVIDDDPAVRQVIAGIGRSVGFEVTGCEGDA
                     SLDDCLGRMSDLIILDLVMPVVDGIEVIERLGENHSAARLVLVSGQDRRVLASASRLA
                     TACGLKVAAVFAKPFSAADLRACLTEQKGAITGGDTARLRRMPPDIRSARIEQALQAQ
                     EIVLHYQPQVSVDTLEWQGVEALVRWQHPEFGLLTPDTFVPLVERNPDLTRRFTHYIL
                     RAAAHQIVPPEAVGGFTGRVGINVTADVLAAADFPNQLIALLEQAGLACERVVLEVTE
                     TTLPSEPVKALAIQTRLRMRGVSLAVDDFGTGHSSLERLHTFPMDELKIDLNFVREST
                     SDPEARAIVHNSIALSRDLRVRCVAEGVENLGSLRLLSKLHCGYAQGYFIGRPMPASE
                     LARWSEQWARRRRDILPQLEAAQ"
     gene            4274..5059
                     /locus_tag="VD737_03995"
     CDS             4274..5059
                     /locus_tag="VD737_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09751.1"
                     /translation="MSDVTGPPGTLTLEVAGEDVADARATLRTTLDAAAMGLLTASVA
                     HAGNNRLTVILSCLDLLHAGNVDADDLRLALDLATGAAQQLATDFGRLLANTRRPTMQ
                     SGAVDLRQALAGAQRLDALLHESALSLAMDVPDGLRVELDRDALVVALLRVLLLARRC
                     GATAVQVAGDALEVPGRTVTLRKGRYCRLSIELAEARLPATLRTAVEPGHVVERLDEP
                     LGLQLAALEAYVGAARGQVLVSEAPDADATRVELYLPASARDP"
     gene            5178..5630
                     /locus_tag="VD737_04000"
     CDS             5178..5630
                     /locus_tag="VD737_04000"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HXW09752.1"
                     /translation="MEDRRCWPLIVGLFLLFVMGTPMADDQEEWVSIAKSDRTELFVK
                     PSTLKWEGQWISVRSKQNFVEPLPSAKKGKTFRSARNEYRIACAHRKLAYSETEAYAE
                     LDLQGAIVQKSRSGEKNLTWMDAPEGTVFGELLEFACDNAPPAPAVAQ"
     gene            5647..6015
                     /locus_tag="VD737_04005"
     CDS             5647..6015
                     /locus_tag="VD737_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HXW09753.1"
                     /translation="MTTSRTFDHPALLDKFGGDEEFVRSLLGVTLRSSGALPTELRHA
                     CAAADFAALATLAHKIKGTAGDIVATTLQSQARGAEVAARAADPGAIGLGLELADTMD
                     EFLGEVRSAVSRQGVPREAR"
     gene            6012..>6321
                     /locus_tag="VD737_04010"
     CDS             6012..>6321
                     /locus_tag="VD737_04010"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXW09754.1"
                     /translation="MTPTATQASVRRREFTTAGLGGLDLHAQAWLPDDHVRSVIVVAH
                     GLGEHSGRYAGLAEALVGAGHAVYALDHRGHGRSPGPRANIGRFEHLVSDYGAFAGRA
                     A"
BASE COUNT          904 a         2218 c         2236 g          963 t
ORIGIN      
        1 atctcgatcc cgctcggctt cctcgcagtg ctgctcggct ccatgctatt ccgcgaccgt
       61 cgctccgaag cgctgtggcc cgaggtctac gcacggcaga acaccggcat cctcgcgtcg
      121 aaggcgacgg cgcactagcg gttagcggtt agcggttagc ggttactccg agcgcgccag
      181 gcgcgccgac gacatcatca tgcgatcgat catgcagcgg tcgtctggga ctacacttcc
      241 gactaaccgc taaccgctaa ccgctgcttg tcttgtctac cccgcctctc atcgtcgacg
      301 cgacggttca gttcctgcgc gcgcacctgc cgttctcgcg catggcgcgc aaggacctcg
      361 agttcgtcgc ggagcgggcg cggctcggct atttcccggt gggcacggtc atcgtcgacc
      421 cggcgagcgg catcgcggaa ttcctctaca tcatccagcg cgggcacgtc cgcgtccgca
      481 acccgaacgc ggcggtcgac gacgaggtcc gcggggcggg cgagtgcttt cccgtgatcg
      541 cgctctcggc cgcgtcggcc ggcagccgga cgttcgaagc gaccgaggac gtcttctgcc
      601 tgctgctgcc gcgcgcggac ttcgacgagc tgcggcggcg ctcggcggag ttcgcgcagt
      661 tctgcaccga ggcgctcgcg acggtggtgc agcattcgct gggccagctg cgtcatcatt
      721 tctcgcagcg tgccgccgac cagcagacgc tgctcgagcc gatcaaggcg ctggtgcggc
      781 gtgatccggt ctattgcctg gtcgacaccc ccgtgcgcgc cgcgctcgag cgaatgcgcc
      841 aggagggcgt gcgtacgatt gcggtcgtgg acaccgggcg acgtccggtc ggagtcttca
      901 cgctgatcga cctgatggaa cgcgtcgtgc tgccgggcgt cacgctgtcg acgccggttt
      961 ccgaggtcat gtccgcctgt ccggtagtgg tcgacgaact gaccaccgcg caggacggac
     1021 tcggcctcat ggcgagccgc ggcttccacc agctgctggt ggtgcagggc ggcgagctca
     1081 ccggcatcgt gaccgagcgc gacctgttcg gcctgcagcg cgtgaccatg cgcaacgtca
     1141 cgcaatcgat ccgcctggcc cgcgacgtgc cggcattgcg ccgctgcgtc gacgacatcc
     1201 agtcgctgac cgagaacctg ctggcgcagg gtgcggccgc cgagccgctc acgcacacga
     1261 tcacggcgct caacgacgcg ctcacgcagc ggctgttcga actgctgctg ccgcagttcc
     1321 cgcgcgtggc cccgctgtcg tggtgctggc tcgcggtggg cagcgagggc cggcgcgagc
     1381 agaccgtggc caccgaccag gacaacgcga tcgtgttccg cgcgaccgct cgagacgtcg
     1441 agccggcgcg cgaagcgctg ctcgagttcg cgcgcgcggc caatcacgcg ctggcggagc
     1501 tcgggtttcc gctctgcgtc ggcaacatca tggcgggcaa cccggactgc tgcctgacgc
     1561 tcgaggagtg gcgcgcgcgc ttcgcgtcgt ggatgcgcga gccgaccccg acggcgctgc
     1621 tgggcgccaa catcttcttc gacttccgcg cgctgtccgg cgacggcacg ctggcggcga
     1681 cgctgcggca gtggctcgat gccacgagcg gcgagaacaa gctgttcctg cgcatgctcg
     1741 tcgccaacgc gctgcaggcc gagccgcccc tcggctggat ccgcacgttc cgcaccgacg
     1801 cgggcgagac gatcgacctg aagacgcacg gcacgaggat cttcgtcgat gcggcgcgtg
     1861 cctttgcgct ggcgctcggc atccccgaga ccaacactgc gcagcgcatc cgcgtggccg
     1921 gtcgccgtct cgcccgcgac gagcgcgaca tcacggcgac cgtggatgcg taccagttcc
     1981 tgcagctgct gcggctgcgc gtccagcgcg gcggtctcga gaccgccgcg gccgtggaag
     2041 cgcccgccga caagggtccg cgggcgccga accgactcga cccttacgcg ctcaacgagc
     2101 tcgaccagcg catgctgcgc gaagcgttcc ggcaggcgcg ggcgctccag acgcacctgg
     2161 accagtcggt cggtcactga cgatgctgct gccgtcgacc ttcggtgaag tgtggcggtc
     2221 ccgtcgcatc cgacgatcgc tgcctgccga actccaggca cgcctgggcc gggtccaccg
     2281 cagcttcctc gaccgcccgg gcctcgacca gccactcgcg ctcgcgcggt tcattgcgct
     2341 cgacctcgaa acgacggggc cgcgcatgct gaccgaccgc atcatctcga tcggtgcggt
     2401 cgcggtcacg gacggcacgg tgcggcacga cgacgcgttc gaggcgctgc tgcggcagga
     2461 gcgctcgagc gcggtcgaca acatcctcgt gcatcagatc gggggccagg cgcagctcgg
     2521 cggcaccgat ccggcagagg cgctggttcg ctgcctcgag ttcgtgggcg attcgcggat
     2581 cgtggcgttc cgggccgagt tcgaccagac ggtgttccgc agggaggcgc ggctgcgact
     2641 gggcgtgcac gcgtggccga ggttcatcga cctcgcggcg ctgctctcgg cgctgtttcc
     2701 ggggacggcc aacgacacgc tcgacgagtg ggtgggtcat ttcggcctgc cgccgatcgg
     2761 ccgccatcac gccatcgcgg atgcctatgc cacggccagg ctgctgatgg tcgtgctgca
     2821 ggcggccgcg agggccggca tcgagacgag ccgggacctg cgcgacgccg agcaggccca
     2881 gcgctggctg gggcgacggc gctgacagac gcccgtcctg ggggccgggc aatccgaacc
     2941 agtttgacgc gggttggcag ggggcgcgga agtgtgaacc aagtcacgtt ttaccatgca
     3001 cggcgttccc aaggccgcgc gagaacccat tgactgccgt tccagtgatg cagccgaacc
     3061 cgcgactgct ggtgatcgac gacgaccctg ccgtgcggca ggtgatcgcc ggcatcggtc
     3121 gctcggtcgg gttcgaggtg accggctgcg agggcgacgc gtcgctcgac gactgcctgg
     3181 gccgcatgtc cgacctgatc atcctcgacc tggtcatgcc ggtcgtggac ggcatcgagg
     3241 tgatcgagcg cctcggtgag aaccactctg cggcgcggct cgtgctggtc tccggccagg
     3301 atcgccgcgt gctggcaagc gcgtcgcgac tggccaccgc gtgcggactc aaggtcgccg
     3361 cggtgttcgc caagccgttc agcgccgcgg acctgcgcgc ctgcctgacg gaacagaagg
     3421 gcgcgatcac gggcggcgac acggcgcggc tgcgccgcat gcccccggac atccgcagcg
     3481 cccgcatcga gcaggcgctg caggcgcagg agatcgtcct gcactatcag ccacaggtga
     3541 gcgtcgacac gctcgagtgg cagggcgtcg aggcgctggt gcgctggcag cacccggaat
     3601 tcgggttgct gacccccgac acgttcgtgc cgctcgtcga gcgcaacccg gacctgacgc
     3661 ggcggttcac gcactacatc ctgcgcgcgg ccgcgcacca gatcgtgccg ccggaggcgg
     3721 tcggcgggtt cacgggtcgc gtcggcatca acgtcacggc ggacgtgctc gccgcggccg
     3781 atttcccgaa ccagctgatc gcgctgctcg agcaggcggg actcgcgtgc gagcgcgtcg
     3841 tgctcgaggt caccgaaacg acgctgccct ccgagccggt gaaggccctg gcgatccaga
     3901 cgcgtctgcg catgcgcggg gtctcgctgg cggtcgacga cttcggcaca ggccactcga
     3961 gcctcgagcg gctgcacacg ttcccgatgg acgagctcaa gatcgacctc aatttcgtgc
     4021 gcgagtcgac cagcgacccg gaggcgcgcg cgatcgtcca caactccatc gctctttcgc
     4081 gtgacttgcg ggtgcgctgc gtcgccgagg gcgtcgagaa cctggggtcg ctccggctgc
     4141 tgtcgaagct gcactgcggt tacgcgcagg gctatttcat cggccggccg atgccggcct
     4201 ccgagctggc gcgatggtcg gagcagtggg cgcgtcgtcg ccgcgacatc ctgccgcagc
     4261 tggaagcggc tcaatgagcg acgtcacggg cccgccaggc acgctcacgc tcgaagtggc
     4321 gggcgaagac gtcgccgatg cgcgcgccac gctgcgcacg acgctcgacg cggcagccat
     4381 gggcttgttg acggcctcgg tcgcgcacgc cggcaacaac cgcctcacgg tgatcctgtc
     4441 gtgcctcgac ctgctgcacg caggcaacgt cgacgcggac gacctgcggc tggcgctcga
     4501 cctcgcgacc ggcgcggccc agcagctcgc gacggatttc ggtcggctgc tcgcgaacac
     4561 ccgccgcccg acgatgcagt ccggggcggt cgacctccgc caggcgctag ccggtgcgca
     4621 gcgtctcgac gcgctgctgc acgaaagcgc gctgtccctg gccatggacg tgccggatgg
     4681 gctgcgcgtg gagctcgatc gcgacgccct cgtcgtggcg ctgctgcgcg tgctgctgct
     4741 cgcgcgtcgc tgcggcgcga cggccgtgca ggtcgcgggg gacgccctcg aggtgccggg
     4801 ccgcacggtc acgctgcgca aggggcgcta ctgccgcttg tcgatcgagc tcgccgaggc
     4861 gcggctgccc gcgacgctcc gaaccgccgt cgagcctggc cacgtcgtcg agcggctcga
     4921 cgagcccctc ggcctgcaac tggccgcgct cgaggcgtac gttggtgccg cgcgcggcca
     4981 ggtgctggtg agcgaggcgc ccgacgccga cgcgacacgg gtcgagttgt acctgccggc
     5041 ctccgctcgc gatccgtaag gtcactgcgg cagacccgtg tttacgacgg cccgctccgg
     5101 tgcaaacatc gtactgccgc acggcctggc cggtacgcca ggccgtcggg tcgtcctcga
     5161 aagttgaggt gatcgtcatg gaagaccgca ggtgctggcc gctgatcgtg ggcctcttcc
     5221 tgctcttcgt catgggcacg cccatggcgg acgaccagga ggagtgggtg tccatcgcga
     5281 aaagcgatcg cacggagcta ttcgtaaagc cttccacgct caagtgggag ggccagtgga
     5341 tcagcgtccg cagcaagcag aacttcgtcg aaccgctgcc gtcggccaag aagggcaaga
     5401 ccttccgttc ggcccgcaac gagtaccgca tcgcctgcgc ccatcgaaag cttgcgtaca
     5461 gcgagaccga ggcctatgcg gagctcgacc tgcagggcgc gatcgtgcag aagtcgcgaa
     5521 gtggcgagaa gaacctgacg tggatggacg ccccggaggg cacggtgttc ggcgagctgc
     5581 tcgagtttgc ctgcgacaat gctccgccgg cgcccgccgt ggcgcagtga ccggggttcg
     5641 agcaccgtga cgacgtccag gaccttcgac cacccggccc tgctcgacaa gttcggcggc
     5701 gacgaggagt tcgtccggtc gctgctcggc gtcacgctgc gatcgagcgg cgcgttgccg
     5761 acggaactgc gccacgcctg cgccgccgcg gacttcgccg cgctcgcgac actggcgcac
     5821 aagatcaagg gcacggccgg cgacatcgtc gccacgacgc tgcagtcgca ggcgcgggga
     5881 gctgaggtcg cggcgcgggc ggccgacccc ggcgccatcg ggctgggcct cgagctggcc
     5941 gacacgatgg acgagttcct tggcgaggtg cgcagcgccg tgtcgcggca gggcgtaccc
     6001 cgggaggcga gatgaccccg accgcgacgc aggcctcggt gcgacgacgc gaattcacga
     6061 ccgccggtct cggcgggctc gacctccatg cccaggcatg gctgccggac gaccacgtgc
     6121 gctccgtgat cgtggtcgcg cacgggctcg gcgagcattc ggggcgctac gcgggcctgg
     6181 ccgaggcgct ggtcggggca ggccacgccg tgtacgcgct cgaccaccgc gggcacgggc
     6241 gctcgcccgg gccccgcgcc aacatcgggc ggttcgagca cctggtctcc gactacggcg
     6301 cgttcgctgg gcgtgccgcg c
//
LOCUS       DASPFR010000159         6401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1268728, whole genome shotgun sequence.
ACCESSION   DASPFR010000159 DASPFR010000000
VERSION     DASPFR010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268728"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VD737_04015"
     CDS             <1..975
                     /locus_tag="VD737_04015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXW09755.1"
                     /translation="TEFWHFLVLGLFVGLAGGSFSVGTPYVARWFDKKHQGFAMGVFG
                     AGNSGAAVTKFVAPAIVVGFGWIFVPQVYAVAMLITALAFWFFTYSDPKHRVPGHVTY
                     RRQLECLRDPNVWKYCQYYSIVFGGYVALSLWMVNYYVGEFGLDIRIAALLAACFSLP
                     GGVLRAIGGWMADKWGAHRVTWWVMWVSWICLFLLAYPATSYTVQTVNGPVTFTLAHN
                     VWSFTFLMFLLGIAWAFGKASVFKYIADDFPTNIGTISGVVGLAGGLGGFVLPILFGA
                     LLDLTGVRSTAFMMMYAVVWVSLIWMYWTEVRRSEVMGPRATEFSLRG"
     gene            1007..2656
                     /locus_tag="VD737_04020"
     CDS             1007..2656
                     /locus_tag="VD737_04020"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09756.1"
                     /translation="MSVATPIPIPPKSASGADIADWRPEDPAFWETTGKKIAYRNLAL
                     SVPALLCGFAVWGMWGIITVQMLNLGFPFTQAELFTLTAIAGIAGATMRIPASFLIRL
                     AGGRNTIFLTTAMLLAPAIGTGVVLQHKDWPLWAFQLMALWSGVGGGNFASSMSNIST
                     FFPKRLQGTALGINAGIGNFGVTTMQIVIPLCMTVGLFGAFAGEPMTLLKDSGWIFGK
                     IAAGTPTWIQNAGFAWVISLVPLSILCWLGMNNLKTVSPDTKVIPSFAKITYLYTLAF
                     VPSILGLYLYLPKPTGLGLISMWVAIPLDVIAALLVMRLTAFGTMKEGIKKQFAIFND
                     KHTWSMTLLYIVTFGSFIGFSMALPLAITVIFGFSHVPDASGVMTHTIKNPNAPSAFT
                     YAWMGPFIGAAIRPVGGWIADKVGGSIVTQIISAVMVAASVAVGYVMLQAYGSATPEK
                     YFLLFMVLFIVLFAASGIGNGSTFRTIGVIFDRTQAGPVLGWTSAIAAYGAFIAPIVI
                     GEQIKAGTPQFAMYGFAIFYGLCLVLNWWFYLRRNAYVKNP"
     gene            2682..>6401
                     /locus_tag="VD737_04025"
     CDS             2682..>6401
                     /locus_tag="VD737_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HXW09757.1"
                     /translation="MSHFLDRLTHFTLPKEQFSNGHGVATGEDRTWEDAYRNRWAHDK
                     IVRSTHGVNCTGSCSWKIYVKGGIVTWETQQTDYPRTRADLPNHEPRGCARGASYSWY
                     LYSANRLKYPMVRGRLLERWRAALRVSKTPVDAWASIVEDPAARRDYQRVRGMGGFVR
                     SSWDEVNQLIAAANVYTIKKHGPDRVIGFSPIPAMSMVSYAAGSRYLSLIGGVCMSFY
                     DWYCDLPPASPQIWGEQTDVPESADWYNSTYIIAWGSNVPQTRTPDAHFFTEVRYKGA
                     KTVAVTPDYSEVAKLADLWMHPKQGTDAALAMAMGHVVLKEFYFGGSTGRPRSAYFDD
                     YARRYTDLPLLVRLKEQTLADGRKVLAPDRYLRASDFADSLGQANNPDWKTVAFDTAG
                     KAVLPNGSIGFRWGAEGRDDAGKWNLESKEAHHGSDVRLKLSLLEDSGPAHEISEVAF
                     PYFAGADTPHFQSNKLAGDLNMARVPAVRLSLADGETLVATVFDLQAAQYGIDRGLGS
                     GAKDYDDNAPYTPAWQERITGVPREQVITVARQFADNADKTRGKSMIIIGAAMNHWYH
                     ADMNYRGVINLLMMCGCIGQSGGGWAHYVGQEKLRPQTGWTALAFALDWIRPPRQQNS
                     TSFFYAHTDQWRYEKLGVEEVLSPLADRKAFGGSLIDYNVRAERMGWLPSAPQLKTNP
                     LQVVRDAQAVGADPKDYVVRALRDGTLQMSCEDPDAPQNWPRNLFVWRSNLLGSSGKG
                     HEYFCKHLLGTENGVQGKDLGKDDAKPTEVTWHDEAPEGKLDLLVTLDFRMSTTCLYS
                     DIVLPTASWYEKNDLNTSDMHPFIHPLSAAVDPVWQSRSDWEIYKGFAKAFSEVCVGH
                     LGVEKEVVLTPLMHDTPAELAQPFDVKEWKRGQCDLIPGKTAPQIAVVERDYPNVYKR
                     FTSLGPLMAKAGNGGKGIGWNTQTEVQQLGELNGVDDAGRPRIVSDVDACEVILQLAP
                     ETNGHVAVKAWEALSKITGREHAHLALHREDEKIRYRDVLAQPRKIISSPTWSGLESE
                     KVSYNAGYTNVHELIPWRTLTGRQQFYQDHPWMIAFGEGFSSYRPPIDLKATSGIHNI
                     KPNGNPEILLNFITPHQKWGIHSTYSDNLMMLTLNRGGPVIWLSEDDAKTAGIEDNDW
                     VELFNINGAIAARAVVSQRVKPGMTMMYHAQEKIINTPGSEITGVRGGIHNSVTRIVT
                     KPTHMIGGYAQYSYGFNYYGTIGTNRDEFVVVRKMR"
BASE COUNT         1159 a         2098 c         2038 g         1106 t
ORIGIN      
        1 accgagttct ggcatttcct ggtgctggga ctgttcgtcg gtctcgccgg cggctcgttc
       61 tcggtcggca ccccgtacgt cgcgcgctgg ttcgacaaga agcaccaagg cttcgcgatg
      121 ggcgtgttcg gcgccggcaa ctcgggggcg gcggtcacga agttcgtcgc gcccgcgatc
      181 gtcgtcggct tcggctggat cttcgtgccg caggtgtacg cggtagcgat gctgatcact
      241 gcgctcgcgt tctggttctt cacctacagc gatccaaagc accgggtgcc cggccatgtg
      301 acctaccgcc gccagctcga gtgcctgcgc gacccgaacg tctggaagta ctgccagtac
      361 tactcgatcg tcttcggcgg ttatgtggcg ctgtcgctgt ggatggtcaa ctactacgtc
      421 ggcgaattcg gcctcgacat ccgcattgcc gcgctgctgg cggcgtgttt ctcgctgccc
      481 ggcggcgtgc tgcgcgccat cggcggctgg atggccgaca aatggggcgc gcaccgtgtc
      541 acctggtggg tcatgtgggt cagctggatt tgcctgttcc tgctcgcgta tccggcaacc
      601 agctacacgg tgcagaccgt caacggtccg gtgaccttca cgctggccca caacgtctgg
      661 agcttcacct ttctcatgtt cctgctcggc attgcctggg cattcggcaa ggcgagcgtg
      721 ttcaagtaca tcgccgacga cttcccaacg aacatcggaa caatttcagg ggtcgtcggg
      781 ctggccggag gcctcggcgg cttcgtgctg ccgatcctgt tcggcgccct gctcgacctc
      841 accggcgtgc gctcgaccgc tttcatgatg atgtacgcgg tggtctgggt gtcgttgatc
      901 tggatgtact ggaccgaagt gcgccgctcg gaagtcatgg gcccgcgcgc caccgagttc
      961 agcttgcgcg gttgattccc tcttcccgag caccgaagga ccgactatga gtgtcgccac
     1021 acccatcccc atcccgccca aatcagcctc gggcgccgac atcgcagatt ggcggccgga
     1081 agaccccgcg ttttgggaaa cgaccggcaa gaagatcgcc taccgcaacc tcgcgctgtc
     1141 ggtgccggcc ctgctgtgcg gcttcgcggt ctggggcatg tggggaatca tcacggtgca
     1201 gatgctgaac ctcggctttc cgttcacgca ggccgagctc ttcacgctga ccgcgattgc
     1261 aggcatcgcc ggcgccacga tgcgcattcc ggcttcgttc ctgatccggc tggctggcgg
     1321 ccgcaacacg atcttcctta cgactgccat gttgctggcg ccggccatcg gcaccggcgt
     1381 cgtgctgcag cacaaggact ggccgctgtg ggcgttccag ctgatggcgc tgtggtcggg
     1441 tgtgggcggc ggcaatttcg ccagctcgat gtcgaacatc agcacgtttt tccccaagcg
     1501 gctgcagggc acggcgctcg gcatcaatgc cggcatcggc aatttcggcg tcacgacgat
     1561 gcagatcgtg atcccgctgt gcatgacagt cggcctcttc ggcgcctttg caggcgagcc
     1621 gatgacactg ctgaaggaca gcggctggat cttcggcaag atcgccgccg gcacgccgac
     1681 ctggatccag aacgccggct tcgcctgggt gatttcgctg gtgccgctgt cgatcctgtg
     1741 ttggctcggc atgaacaact tgaagacggt gtcgcccgac accaaggtga tcccgtcgtt
     1801 cgccaagatc acctacctct acacgctcgc cttcgtaccg tcgatcctcg gcttgtattt
     1861 gtatctgccg aagccgaccg gactcgggct gatcagcatg tgggtggcga tcccgctcga
     1921 tgtaatcgcg gcgctgctgg tgatgcggct gactgcattc ggcacgatga aggagggcat
     1981 caagaaacag ttcgcgattt tcaatgacaa gcacacgtgg tcgatgacgc tgctttacat
     2041 cgtcacgttc ggctcgttta tcggcttctc gatggcgctg ccgctcgcga tcaccgtgat
     2101 ctttggcttc tcgcacgtgc cagacgccag tggcgtgatg acgcacacga tcaagaatcc
     2161 gaacgcacct tcggcgttca catacgcgtg gatgggtccg ttcattggcg ccgcgatccg
     2221 gccggtcggc ggctggatcg ccgacaaggt cggcggctca atcgtcacgc agatcatctc
     2281 ggcagtgatg gtcgccgcgt cggtggccgt cggctacgtg atgctgcagg cgtacggctc
     2341 ggccacgccg gaaaagtact tcctgctctt catggtgctg ttcatcgtgc tgttcgccgc
     2401 cagcggaatc ggcaacggct cgacgttccg caccatcggc gtgatcttcg accgcacaca
     2461 agccggtccg gtcctcggct ggacctccgc catcgccgcg tacggcgcgt tcatcgcgcc
     2521 gatcgtgatc ggcgaacaga tcaaggccgg aacgccgcag ttcgcgatgt acggattcgc
     2581 catcttttac ggcctgtgcc tggtcctgaa ctggtggttc tacctgcgcc gcaacgccta
     2641 cgtgaagaac ccctgacatt cccagccgcg gagacacatc aatgagtcac tttctcgatc
     2701 gactgaccca cttcacgctg ccaaaggagc agttctccaa cggccatggc gtggctaccg
     2761 gggaggatcg cacctgggag gacgcctacc gcaatcgctg ggcgcacgac aagattgtgc
     2821 gcagcacgca cggcgtcaac tgcaccggtt cctgctcctg gaagatctac gtcaagggtg
     2881 gaatcgtgac gtgggaaacc cagcagaccg actacccgcg cacgcgcgcc gacctgccaa
     2941 accacgagcc gcgtggatgc gcgcgcggcg cgtcgtactc gtggtacctg tacagcgcca
     3001 accgtctgaa gtacccgatg gtgcgcggcc gcctgctgga gcgctggcgc gccgcgctac
     3061 gagtgtcgaa gacgccggtc gacgcgtggg caagcatcgt cgaggatccg gccgcgcgcc
     3121 gcgactacca gagggtgcgc ggcatgggag gcttcgtgcg cagctcatgg gacgaggtca
     3181 accaactgat cgccgcggct aacgtgtaca cgatcaagaa gcacgggccg gaccgcgtga
     3241 tcggcttctc gccgatcccg gccatgagca tggtcagcta cgccgccggc agccgctatc
     3301 tttcgctgat cggcggcgtc tgcatgagtt tctacgactg gtactgcgac ctgccgcccg
     3361 ccagcccgca gatctggggt gagcagaccg atgtcccgga gtcggccgac tggtacaact
     3421 caacctacat catcgcctgg ggttcgaacg tgccccagac ccgcacgcct gacgcgcact
     3481 tcttcaccga ggtccgctac aagggtgcga agacggttgc agtcacgccc gactattccg
     3541 aagtcgccaa gctggccgac ctctggatgc accccaagca gggaaccgac gccgcgctcg
     3601 caatggcgat gggccacgtt gtgctgaagg agttctactt cggcggcagc acggggcgtc
     3661 cacgctcggc gtacttcgac gactacgcgc gccgctacac cgacctgccg ctgctggttc
     3721 gcctgaagga acagacgctc gcagacggcc gcaaggtgct cgctcccgac cgctacctcc
     3781 gcgccagcga cttcgcggac agcctcgggc aggccaacaa tcccgactgg aagacggtgg
     3841 cgttcgacac ggccggcaag gcggtcctgc ccaacggctc gatcggcttc cgctggggtg
     3901 ccgaaggccg cgacgacgca ggcaagtgga acctcgagtc gaaggaagcg caccacgggt
     3961 ctgacgtgcg gctaaagctg tcgctcctgg aggacagcgg gccggcgcac gagatcagtg
     4021 aggtcgcctt cccctacttc gccggcgccg acacgccgca cttccagagc aacaagctgg
     4081 caggcgatct gaacatggcg agggtgcccg cggtccggct gtcgctcgcg gacggcgaga
     4141 cgctggtcgc gacggtgttc gacctgcagg cggcccagta cggcatcgac cgcgggctcg
     4201 gcagcggcgc gaaggactac gacgacaacg cgccgtacac cccggcctgg caggaaagaa
     4261 tcaccggtgt gccgcgcgaa caggtcatca cggtcgcccg gcagttcgcg gacaacgccg
     4321 acaagacccg cggcaagtcg atgatcatca tcggtgccgc tatgaaccac tggtaccacg
     4381 ccgacatgaa ctaccgcggc gtgatcaacc tcttgatgat gtgcggctgc atcggccaga
     4441 gcggcggcgg ctgggcgcac tacgtcgggc aggaaaaact ccgcccgcag accggctgga
     4501 ccgcactcgc gttcgcgctc gactggatcc gtccgccgcg ccagcagaac tcgacctcgt
     4561 tcttctacgc gcacaccgac cagtggcgct acgagaagct cggcgtcgag gaggtgctgt
     4621 cgccgctggc cgaccgcaag gccttcggcg gcagcctgat cgactacaac gtgcgcgccg
     4681 agcgcatggg ctggctgccg agcgcgccac agctgaagac caacccgctg caggtcgtgc
     4741 gcgacgcaca ggcggtcggc gcggacccaa aggactacgt cgtgcgcgct ctgagggacg
     4801 gcacgctgca gatgagctgc gaggacccgg acgcgccgca gaactggccg cgcaacctgt
     4861 tcgtgtggcg ctccaacctg ctcggctcga gcggcaaggg gcatgagtat ttctgcaagc
     4921 acctgctcgg caccgagaac ggcgtgcagg ggaaggacct tggcaaggac gacgcgaagc
     4981 ccaccgaagt cacgtggcac gacgaggcac cggaaggcaa gctcgacttg ctggtcacgc
     5041 tggacttccg catgagcacc acgtgcctgt acagcgacat cgtgctgccc acggccagct
     5101 ggtacgagaa gaacgatctc aacaccagcg acatgcatcc gttcatccac ccgctcagcg
     5161 cggcagtgga cccggtatgg cagtcgcgca gcgactggga gatctacaag ggatttgcga
     5221 aggcattttc cgaggtctgc gttggccatc ttggcgtcga gaaggaagtg gtgctgacgc
     5281 cgctgatgca cgacacgccg gccgagctgg cgcagccgtt cgacgtgaag gagtggaaga
     5341 gggggcagtg cgacctgatc cctggcaaga ccgcgccgca gattgcggtg gtcgagcgcg
     5401 actacccgaa tgtctacaag cgcttcacgt ccctcggacc gctgatggcc aaggcgggca
     5461 acggtggcaa gggcatcggc tggaacaccc agaccgaggt gcagcaactc ggcgaactga
     5521 acggcgtcga cgacgctggc cggccgcgca tcgtcagcga cgtcgatgcc tgcgaggtca
     5581 tcctgcagct cgccccggag accaacggcc acgtggcagt gaaggcctgg gaggcgctgt
     5641 cgaagatcac cggccgcgag catgcacacc tcgcgctgca ccgcgaggac gagaagatcc
     5701 gttaccgcga cgtgctggcg cagccccgca agatcatcag ctcgccgacc tggtcggggc
     5761 tcgagagcga aaaggtctcg tacaacgccg gttacaccaa cgtgcacgaa ctgattccgt
     5821 ggcgcacgct cactggccgg cagcagttct accaggacca cccctggatg atcgcgttcg
     5881 gcgagggctt ctccagttac cggccgccga tcgaccttaa ggccacctcg ggcatccaca
     5941 acatcaagcc caacggcaac ccggagatcc tgctcaactt catcacaccg caccagaagt
     6001 ggggcatcca ctcgacctac agcgacaacc tgatgatgct gacgctgaac cgcggcggcc
     6061 cggtgatctg gctatcggag gacgacgcga agacggccgg catcgaggac aacgactggg
     6121 tggaactgtt caacatcaac ggcgcgatcg cggcacgcgc ggtcgtcagc cagcgcgtca
     6181 agcccggcat gaccatgatg taccacgcgc aggagaagat catcaacacg ccgggctccg
     6241 agatcacggg cgtgcgcggc ggcatccaca actcggtcac gcgcatcgtg accaagccga
     6301 cgcacatgat cggcggctac gcgcagtaca gctacggctt caactactac gggacgatcg
     6361 gcaccaaccg tgacgagttc gtcgtcgtgc gcaagatgcg g
//
LOCUS       DASPFR010000160         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_169138, whole genome shotgun sequence.
ACCESSION   DASPFR010000160 DASPFR010000000
VERSION     DASPFR010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169138"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2161
                     /gene="uvrA"
                     /locus_tag="VD737_04030"
     CDS             <1..>2161
                     /gene="uvrA"
                     /locus_tag="VD737_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004797750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXW09758.1"
                     /translation="SLSAYARQFLEQMEKPEVDSIEGLSPAISIEQKTTSRNPRSTVG
                     TVTEIYDYLRVLYARAGVPRCPEHGQDLAAQTVTQMVDQVLALPEGTGVLLLAPIVQD
                     RKGEHAEVFEHLRSQGYVRARIDGTLHELDERPALDPRKRHSIEAVIDRLKVRPDAAQ
                     RLAESFETALALAQGTARLAFHDDPGREPLQFSNRFACTICGYSLTELEPRIFSFNNP
                     NGACGTCGGLGLQEFFDPARVVVNPSLSLAGGAIRGWDRRNAYYFQLIQALARHFRFD
                     IEAPWQELPEAVRHALLLGSGEEKIAFRYFDAKGAQTRRTHAWEGILPNLERRYRETE
                     SATVREELAKYRGTRPCVDCGGSRLNQAARNVFVAGRALPEIAALSIADARAFFGGVQ
                     IEGWRGEIAARLVREVGDRLKFLSDVGLDYLSLDRSAETLSGGEAQRIRLASQIGSGL
                     VGVMYILDEPSIGLHQRDNQRLLDTLVRLRDLGNTVLVVEHDEDAIRQADYVVDLGPG
                     AGSHGGLVVAQGTPELVANHPQSLTGAYLSGRRRIPVPAQRRTSQPDRSIRIVGATGN
                     NLRGVNATFPLGLMTCVTGVSGSGKSTLVVDTLYRHAAGAMQESAGEAAPCVAVEGLD
                     QIDRVIDIDQSPIGRTPRSNPATYTGIFGPVRELFAEVPEARARGYDPGRFSFNVRGG
                     RCEACEGDGVIRVEMHFLPDAYVTCDACRGMRYNRETL"
BASE COUNT          327 a          768 c          748 g          318 t
ORIGIN      
        1 tccctctccg cctacgcgcg gcagttcctc gagcagatgg agaagccgga ggtcgactcc
       61 atcgagggcc tgtcgcccgc catctcgatc gagcagaaga ccacctcgcg gaatccccgg
      121 tcgaccgtgg gcaccgtcac cgagatctac gactacctgc gcgtgctcta cgcgcgggct
      181 ggcgttccgc gctgtcccga gcacggacag gacctcgcgg cgcagaccgt cacgcagatg
      241 gtcgaccagg tgctcgcgct gccggaaggc acgggcgtgc tgctgctcgc gccgatcgtg
      301 caggaccgca agggcgagca cgccgaagtt ttcgagcacc tgcgcagcca ggggtacgtg
      361 cgggcccgca tcgacggcac gctgcacgag ctcgacgagc ggccggcgct cgatccgcgc
      421 aagcggcact cgatcgaggc cgtgatcgac cgtctcaagg tgcgtcctga cgccgcgcag
      481 cgactcgcgg agtcgttcga gacggcgctc gcgctcgcgc agggcacggc gcggctcgcc
      541 ttccacgacg atccggggcg cgagccgctg cagttctcca accgcttcgc gtgcacgatc
      601 tgcggctaca gcctcacgga actcgagccc cggatctttt cgttcaacaa tccgaacggc
      661 gcctgcggca cctgcggcgg gctgggactg caggagttct tcgaccccgc gcgggtggtc
      721 gtcaatccga gcctgtcgct ggcgggcggt gcgattcgcg gctgggaccg tcgcaacgcg
      781 tactacttcc agctgatcca ggcgctcgcg cggcacttcc gcttcgacat cgaggcgccg
      841 tggcaggagc tgcccgaagc ggtgcgtcac gcattgctgc tgggcagcgg cgaggaaaag
      901 atcgcgttcc ggtacttcga cgccaagggc gcgcagacgc gccgcacgca cgcctgggaa
      961 ggcatcctgc cgaacctcga gcgtcggtac cgcgagacgg aatcggccac ggtccgcgag
     1021 gagctcgcca agtaccgtgg cacgcggccc tgcgtcgact gcggcggcag ccggctcaac
     1081 caggccgcgc gcaacgtgtt cgtggcgggt cgcgcgctac cggagatcgc cgcgttgtcg
     1141 atcgccgatg cgcgcgcgtt cttcggcggc gtgcagatcg agggctggcg cggcgagatc
     1201 gcggctcgcc tggtgcgcga ggtcggggac cgcctcaagt tcctgagcga cgtcggcctc
     1261 gattacctgt cgctcgaccg cagtgccgaa accctctccg gcggcgaggc gcagcgcatc
     1321 cggctcgcca gccagatcgg ctcggggctc gtcggcgtca tgtacattct cgacgaaccc
     1381 tcgatcgggc tgcaccagcg cgacaaccag cggctcctcg acacgctggt gcgtctgcgc
     1441 gacctcggca acacggtgct ggtcgtcgag cacgacgagg acgccatccg ccaggcggac
     1501 tacgtggtcg acctcggtcc cggggcaggc tcgcacggcg ggctcgtggt cgcgcagggc
     1561 acgccggagc tggtcgcgaa ccatccacag tcgctcaccg gcgcttactt gagcggacgg
     1621 cgccgcatcc ccgtgccggc gcagcggcga acgtcgcaac cggaccggtc gatccggatc
     1681 gtcggcgcca ctggcaacaa cctgcgaggc gtcaacgcga cgtttccgct cggcctgatg
     1741 acctgcgtca cgggcgtgtc gggctcgggc aagtccactc tcgtggtcga cacgctctac
     1801 cggcacgcgg ccggtgcgat gcaggagtcc gcgggcgaag cggccccgtg cgtggccgtc
     1861 gaagggctcg accagatcga ccgtgtgatc gacatcgacc agtccccgat cggccgcacg
     1921 ccgcgctcga accccgccac gtacacgggc atcttcgggc cggtgcgcga gctgttcgcg
     1981 gaagtgcccg aagcgcgcgc ccgtggctac gaccccggtc gattcagctt caacgtccgc
     2041 ggcgggcgct gcgaggcctg cgagggcgac ggcgtgatcc gcgtcgagat gcacttcctg
     2101 ccggacgcct acgtgacctg cgatgcctgc cgcggaatgc gctacaaccg cgagacgctc
     2161 g
//
LOCUS       DASPFR010000161        10494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1269293, whole genome shotgun sequence.
ACCESSION   DASPFR010000161 DASPFR010000000
VERSION     DASPFR010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269293"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VD737_04035"
     CDS             <1..414
                     /locus_tag="VD737_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09759.1"
                     /translation="RNGVKWWQWDPTKWLIAGLSYVKLATNLKRVPDVQIQRAQLAMQ
                     FKRVEQALEKRRSRSTASDEHLERLKARVAVEYAAFRKAAEEWNKVREEWVADRKQRL
                     LQRWEETSFRLRLKQIEHGMRLQRRRLRAMSKAYA"
     gene            complement(558..2672)
                     /locus_tag="VD737_04040"
     CDS             complement(558..2672)
                     /locus_tag="VD737_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HXW09760.1"
                     /translation="MTGVLLGGLAPVVHAADEASLEPVITVIGDRIRLDTIPGSASVL
                     DATVIQDARVLTVNEAMRKVPGVFARDEEGFGLRPNLGIRGLNPTRSSKLLLLEDGIP
                     LGYAPYGDNASYYHPPVERFERIEVLKGSGQILFGPHTVGGVINYITPAVPGEATGRV
                     MASVGTEGFRELHGQFGDRFGDTGFVAHGTYKQSDGARDNMHFEVGDLNLKVVHELGE
                     RQALTLRGSWYDESSQVTYSGLTLAEWRDDPYQNPFKNDHMYARRWGTSATHRFDLDD
                     AVTFTTNIYYTRFERDWWRQSSNSAQRPNDASDPACGGMASLSATCGNEGRLREYWTA
                     GIEPRVTVEHALFGLDNVLEGGLRYHREDQARIQANGDGPRSRRAGTGVNAGIREDSD
                     RTVEAMSAFVQNQFDLGRWTVTPGVRFESVDYERTDNLAGATGESSIDEVVPGLGATF
                     EAVPGTVLFAGVHRGFAPPGVADIVTSAGGSVDLDAELSWNYELGVRGTAGAGLSYEA
                     TLFRMDFENQIVPASVAGGAGATLTSAGETLQQGLELSAAVDSTAWVDWPVELFARGA
                     YTWLADAEYVGERYSNVPGFGTVRVTGNRLPYAPEHLMSATVGARLPFGLQVALEGVY
                     TSSAYTDDLNTVAIVDSGQRGEMPGYTVWNLTANYALPACDCSVFAAAKNLGDKLYVA
                     DMSRGLIPGMPRTVQAGFEFRF"
     gene            2893..3375
                     /locus_tag="VD737_04045"
     CDS             2893..3375
                     /locus_tag="VD737_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019823277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HXW09761.1"
                     /translation="MPSFDVVSEVEAHEVTNAVDQASREVGQRFDFKGTNAKFELKDG
                     VITLSAPADFQLKQMLEILRLKLSKRGIDVACLKVDEPVVPGQTAKQVVTLRQGVDAE
                     LGKKIQRQIKDSKLKVQAALQDKQVRVTGKNRDDLQAAIALIRSGGYDLPLQFTNFRD
                     "
     gene            complement(3356..4657)
                     /locus_tag="VD737_04050"
     CDS             complement(3356..4657)
                     /locus_tag="VD737_04050"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXW09762.1"
                     /translation="MRTAFTDAREWHVCRVAFALVVAGLGVAACSKPASKDLQPGSYR
                     AVLELPGGKALPFGLDVAREENGPVLYLINGTERVRVTEVSTEPGTLGARMPGYETTL
                     EATVEGGELAGTVRLVHDRGRVLELPFEAKLGETWRFHAEPLSDNADVAGRWQMVFTD
                     ESGRRVRGVAELQQQFEQVTGTVILPTGDQRFLAGDVRDETLQLSRFDGGAAVLYEAK
                     LDTKGNLVGETWSDRGGRQRFVATRDPDAEVESSAAATELRNPDAEFAFSFPDLAARA
                     VSTSDPTFDGRVLLVSITGSWCPNAHDEARLLADLDRRYRSRGLAIVALMFEQHDDPA
                     RAVAAIRRFRDATGIDYPTLLAGGADKAKAAAALPQLEAVRAYPTTVFIDRAGRVRRI
                     QAGFIGPAAEVHHAQLVHEYEQTVEDLLLEPAPDPANPGSS"
     gene            complement(4665..5051)
                     /locus_tag="VD737_04055"
     CDS             complement(4665..5051)
                     /locus_tag="VD737_04055"
                     /inference="COORDINATES: protein motif:HMM:NF022526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2780 domain-containing protein"
                     /protein_id="HXW09763.1"
                     /translation="MDDLIRKLTKDLGVSREQARGGLVALLRTGQQNMSRPDFEQFVA
                     DIPGADKLLRTAPAQSTLSSLAGGLGSLLGGRSGAGRWAGLAASFTELGIDIDTAKRF
                     GPIVIEYVREHGGENLVGKIREALNI"
     gene            complement(5095..7476)
                     /locus_tag="VD737_04060"
     CDS             complement(5095..7476)
                     /locus_tag="VD737_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09764.1"
                     /translation="MALPNYEKLGVFYLGREYDQATGQVGPELLYDARDLTTHAVCIG
                     MTGSGKTGLCLSLLEEAAIDGIPAIAIDPKGDIANLALTFPQLRAEDFKPWVDQGEAA
                     RKGQSLDDFAAATAATWRRGLEEWGQDGTRIQRLRDAAAVTVYTPGSSAGRPLSILRS
                     FAAPDAAVAGDATALKERVGSAVAGLLGLLDIEADPIRSREYILLCALLDAAWRKGES
                     LDLAALIRSVQKPPFEQVGVFDLESFYPAKDRTELALRINALLASPGFEAWLQGDPLD
                     VPSMLYDADGRPRIAIVSIAHLNDAERMFVVTLIANELVAWMRRQPGTTSLRALFYMD
                     EVFGYFPPSAMPPSKTPLLTLMKQARAFGVGVVLATQNPVDLDYKGLGNAGTWFIGRL
                     QTERDKLRVIEGLLGSNAAAGMDKTQLEAAMSNLPQRTFLMRNVHDDAPVLLRTRWAL
                     SYLRGPLTLAEITRLGAAGGAPSPAVARVEAGVPAPAPSPKPAATSSSRPVVPAGLPE
                     QFLAASGTAVEYEPRLGARVRTHFVDARLGVDLWTDTYRLAPLDERGPAWERAESCDR
                     DALDLRDEPSPDAAFAPVPGGVLAAPKALQQHARALVEHVYRTSTLAVLRCPELKAAA
                     TPQQTEGEFRAQLAQSLREKRDRAVDDLRRKYASRLATLTDRQRRAEQKVARERSQAS
                     HETMSSALSVGGSLLGALFGGGRRASALGKAATAARGVGRIGKERTDVAHAEADLTSV
                     REQYAALEAELEAEVSRLESTLDPAAIQLETVTVKPRKSDIEVAELALVWHPA"
     gene            7716..9443
                     /locus_tag="VD737_04065"
     CDS             7716..9443
                     /locus_tag="VD737_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXW09765.1"
                     /translation="MSIALDQVTKRYQGVPVVNDVSLSVRPGEFLVLLGPSGSGKSTL
                     LRAIAGLTDIDHGRISLHGKDVTHLTARQRQVGFVFQHYALFRHMTIGDNIEFALRVR
                     KVRAAERRARRKELLKLVALEGMDDRLPAQLSGGQQQRVAVARALAHRPEVLLMDEPF
                     GALDAKIREELRRTIRQIQRELEITTILVTHDQEEAFALADRIGVMHQGRLLECGRPD
                     DLYTRPATRFVSTFLGAANLLLGYRTSRGVRFHARGIERPHEVVAVLRPEEVELAADE
                     SGIRANFVGHGVLEEVLFGGAVERLRVRMASDGPVPAGSNESHQRRGLLLEVSRTLPE
                     QRDFPVSHGLRVAVGARRIHVLPTPISSFTAVGADEASARAIADSPLLATLGARMQTR
                     VGQQVGTAQRAPPGMPVLATGPGSAETARWQLRNGATQLLCIPPTAPLPHHVLIHTLD
                     ANSRRATLAVAASLLRHIAAEAVYLVIHPTPSLEAERAAHMRDLLDARSSALNDHGLD
                     MRTEMRPGEVTEELQRELAAYEDSMLVLGTSNPEALRWDWLASVLEGEPARPVLIVSS
                     ARVEAPVEA"
     gene            9447..10295
                     /gene="cysT"
                     /locus_tag="VD737_04070"
     CDS             9447..10295
                     /gene="cysT"
                     /locus_tag="VD737_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HXW09766.1"
                     /translation="MATSWFRQPSILPGFGLTLGFTTFFLSALVLLPLAALIFKTTAM
                     TWSEFVSTVLDERAVASYRLSFGAALVAALVNAFFGFIVAWSLVRYEFPGRRLVDALI
                     DLPFALPTAVSGIALAAVFSPTGWLGVRLQEWFGVQVAYTWLGIVVALTLIGLPFVVR
                     SVQPALIEAQRDLEEAAETLGAGPFYVFRRIVLPAVLPALVTGFTMAFARGVGEYGSV
                     VFISGNLPLKTEITPLLIVIKLEQFDYNGAAALGFSMLLVSFLMLFTINLAQAWSRRR
                     HVGAGR"
BASE COUNT         1538 a         3661 c         3637 g         1658 t
ORIGIN      
        1 cgcaacggcg tgaagtggtg gcagtgggac ccgaccaagt ggctgatcgc aggtctctct
       61 tacgtgaagc tggccaccaa cctgaagcgc gtgccggacg tgcagatcca gcgtgcgcaa
      121 ctcgcgatgc agttcaagcg cgtcgagcag gcgctcgaga aacgccgcag ccgcagcacg
      181 gcgagcgacg agcatctcga gaggctcaag gctcgcgtcg ccgtggaata cgccgcgttc
      241 cgcaaggccg ccgaggagtg gaacaaggtc cgcgaggagt gggtggccga ccgcaagcaa
      301 cgactgctgc agcgctggga agagaccagc ttccggctgc gcctcaagca gatcgagcac
      361 ggcatgcgcc tgcagcgccg gcgcctgcgg gcgatgagca aggcgtacgc ttaggaagcg
      421 gttagcggtt agcggtcagt ggctggtcgc tggcaagaag ggcgccgtag atcggcgccc
      481 atttttttcg ttgaccaccg acccatccgc tgtctttcct cactagccac tagccactag
      541 ccactggcca ctggccacta gaatctgaac tcgaatcccg cctggaccgt gcggggcatg
      601 ccggggatca gcccgcggct catgtccgcg acgtagagct tgtcgccgag gttcttggcg
      661 gccgcgaaca ccgagcagtc gcaggccggc agcgcatagt tcgcggtcag gttccagacg
      721 gtgtaccccg gcatctcgcc gcgctggccg ctgtcgacga tcgcgacggt gttcaggtcg
      781 tcggtgtagg cggagctcgt gtagacgcct tcgagggcca cctgcagccc gaacggcagc
      841 cgggcgccga cggtcgccga catcaggtgc tcgggcgcgt acggcagccg gttgccggtc
      901 acccggaccg tgccgaagcc cggaacgttg ctgtaccgct cgccgacgta ctcggcatcc
      961 gccagccacg tgtaggcgcc acgcgcgaac agctcgaccg gccagtcgac ccaggccgtg
     1021 ctgtcgacgg ccgcggacag ctcgagcccc tgctgcagcg tttcgccggc gctcgtcagc
     1081 gtggccccgg ctccgccggc gacgctcgcc gggacgatct ggttctcgaa gtccatccgg
     1141 aacagcgtcg cctcgtacga cagaccggcg ccggccgtgc cccgaacgcc gagctcgtag
     1201 ttccacgaca gttccgcatc gagatcgacg ctacctccgg cggacgtgac gatgtctgcg
     1261 accccgggag gggcgaatcc ccggtgcacg ccggcgaaca gcacggtgcc cggcaccgcc
     1321 tcgaacgtcg cgccgagacc cggcacgact tcgtcgatgc tcgattcgcc cgtggcgccg
     1381 gcgaggttgt cggtgcgctc gtagtcgacg ctttcgaatc ggacgcctgg cgtcaccgtc
     1441 cagcgaccga ggtcgaactg gttctgcacg aacgccgaca tcgcctcgac ggtgcgatcg
     1501 ctgtcttcgc ggatgccggc gttgacgccg gtgccggccc ggcgcgagcg cgggccgtcg
     1561 ccgttggcct ggatgcgtgc ctggtcctcc cggtggtaac gcagcccgcc ctcgagcacg
     1621 ttgtcgaggc cgaacagcgc gtgctcgacc gtgacgcgag gctcgatgcc ggcggtccag
     1681 tattcgcgca gccgcccctc gttgccacag gtcgcagaca ggctggccat gccgccgcac
     1741 gcggggtcgc tcgcgtcgtt ggggcgctgc gccgaattgc tcgattgccg ccaccagtcg
     1801 cgttcgaagc gcgtgtagta gatgttcgtg gtgaacgtca ccgcgtcgtc gagatcgaag
     1861 cgatgcgttg cagacgtgcc ccagcgccgg gcgtacatgt gatcgttctt gaaagggttc
     1921 tggtatgggt cgtcgcgcca ctcggccagg gtgagtcccg agtaggtcac ctgggacgac
     1981 tcgtcgtacc agctgccgcg cagcgtcaac gcctggcgtt cgccgagttc gtggaccacc
     2041 ttgaggttca ggtcgccgac ctcgaagtgc atgttgtcgc gagcgccgtc ggattgcttg
     2101 tacgtgccgt gcgcgacgaa gccggtgtcg ccgaaacggt cgccaaactg gccgtgcaac
     2161 tcacggaacc cctcggtgcc gaccgaggcc atgacacgtc cggttgcctc gccgggcacg
     2221 gcgggcgtga tgtagttgat gacgccgccg acggtgtgcg gcccgaacag gatctggccg
     2281 gagcctttca gcacctcgat gcgctcgaaa cgctcgaccg gcgggtggta gtagctcgcg
     2341 ttgtccccgt acggggcata acccagcggg atgccgtcct cgagcagcag cagcttgctc
     2401 gaacgcgtcg ggttgagtcc gcggatgccg aggttcggtc gcaggccgaa gccctcctcg
     2461 tcgcgtgcga acacgcccgg caccttgcgc atcgcctcgt tgaccgtcag cacacgggcg
     2521 tcctggatca cggtggcatc gagcaccgaa gccgaaccgg gaatcgtgtc cagccgtatg
     2581 cgatcgccga tgacggtgat gaccggttcg aggctggcct cgtcggcggc gtggacgacc
     2641 ggggcgagcc cgccgagcag gacgcccgtc accgcgatgg cgagagcccg cgaagtgggt
     2701 cgattagggg tgacgagcgc catgacgaat cccaagtggc caatgatgag gcgggacttt
     2761 gcttcgcctg gttacaagag tcaatgctaa tcattcgcat tcgcatctgg atatctacgt
     2821 gtccagcggc gtcgtgtcac gcggaggccg ctcgcggtag tctgcgcacc ccgaaaaccc
     2881 gagtgacaac gcatgccatc gttcgacgtc gtctccgaag tcgaggccca cgaggtcacc
     2941 aacgccgtcg accaggcctc acgggaggtc ggccagcggt tcgacttcaa ggggacgaat
     3001 gccaagtttg aattgaaaga cggggtcatc acgctcagtg ccccggcgga ctttcaactc
     3061 aagcagatgc tcgagatcct gcggctgaag ctctccaagc gcggcatcga cgtcgcctgc
     3121 ctcaaggtcg acgagccagt cgtgcctggc cagaccgcga aacaggtcgt cacgctgcgg
     3181 cagggtgtcg acgccgagct cggcaagaag atccagcggc agatcaagga ctcgaagctc
     3241 aaggtccagg ccgcgctgca ggacaagcag gtccgggtca ccggcaagaa ccgggacgac
     3301 ctccaggccg ccatcgcgct gatccgcagc ggcggctacg acctgcccct gcagttcacg
     3361 aacttccggg attagccgga tcgggggcgg gctccaggag caggtcctcg accgtctgtt
     3421 cgtattcgtg caccagttgc gcgtgatgga cctcggccgc ggggccgatg aagcccgcct
     3481 ggatccgacg cacgcggccg gcgcgatcga tgaacacggt ggtcggatag gcgcgcacgg
     3541 cctcgagctg cggcagcgcc gccgcggcct tggccttgtc cgcgccgccg gccagcagcg
     3601 tcggatagtc gatgcccgtg gcgtcgcgaa atcgacggat cgcggcgacg gcccgcgcgg
     3661 ggtcgtcgtg ctgctcgaac atcagggcga cgatggcgag tccgcgcgag cggtagcgcc
     3721 ggtcgaggtc cgccagcagt cgagcctcgt cgtgcgcatt ggggcaccag ctgccggtga
     3781 tcgacacgag cagcaccctg ccgtcgaacg tcgggtcgct cgtcgacacg gcccgggctg
     3841 ccaggtcggg aaacgaaaag gcaaactccg cgtcggggtt gcgcagctcg gtggccgccg
     3901 cgctcgactc gacctcggca tcgggatcac gcgtcgcgac gaaacgctgg cggccccctc
     3961 gatccgacca cgtttcgccg accaggttgc ccttcgtgtc gagctttgcc tcgtagagca
     4021 cggccgcgcc gccgtcgaag cgggacagct gcaacgtctc gtcgcggacg tcgccggcca
     4081 ggaaccgctg gtccccggtc ggcaggatca cggtaccggt gacctgctcg aactgctgct
     4141 gcagttcggc gacgccccgc acgcgccggc cagactcgtc ggtgaacacc atttgccagc
     4201 gaccggccac gtcggcgttg tcgctgagcg gctcagcgtg gaaccgccac gtctcgccga
     4261 gcttcgcctc gaacggcagc tcgagcacgc ggccgcggtc atgcacgagc cggaccgtcc
     4321 cggccagctc gccgccctcg acggtcgcct ccagcgtggt ctcgtagccg ggcatgcgcg
     4381 cgcccagggt gccgggctcc gtcgagactt ccgtcacgcg cacacgttcc gtgccgttga
     4441 tcaggtacag caccggaccg ttttcttcgc gcgcgacgtc gaggccgaac ggcaaggcct
     4501 tgccccccgg caactcgagc actgctcggt agctgccagg ctgcaggtcc ttgctcgccg
     4561 gcttgctgca ggcggcaaca ccaagacctg ccacgacgag ggcgaacgcg acacgacaga
     4621 cgtgccactc gcgtgcgtcg gtgaacgcgg tccgcacggc gtcgtcagat gttgagcgct
     4681 tcgcggatct tgccgacgag gttctcgccg ccgtgctcgc gcacgtactc gatcacgatc
     4741 gggccgaaac gcttggcggt gtcgatgtcg atgccgagtt cggtgaacga ggccgccagg
     4801 ccggcccagc gaccggcgcc cgaccgaccg ccgagcagcg agcccaagcc gccggccagc
     4861 gagctgagcg tgctctgcgc aggtgcggtg cggagcaact tgtccgcgcc cggaatgtcg
     4921 gcgacgaact gctcgaagtc gggacggctc atgttctgct gcccggtccg cagcagcgcc
     4981 accaggccgc cgcgcgcctg ctcgcggctg acgccgaggt ccttggtgag cttgcggatc
     5041 aggtcgtcca tcgctagaag tccgtcgcgc cgggcgaagt gccgcgattc tacgtcatgc
     5101 cgggtgccag accagcgcga gctcggcgac ctcgatgtcg gacttgcggg gcttgaccgt
     5161 caccgtctcg agctggatcg ccgccggatc caacgtcgat tcgagacgtg agacctcggc
     5221 ctcgagttcg gcctctagcg ccgcgtattg ttcccgcacc gaggtcaggt cggcttcggc
     5281 gtgcgcgacg tcggtgcgct ccttgccgat ccggccgacg ccgcgcgcgg cagtggcggc
     5341 cttgcccagt gcgctggcgc gacggccgcc gccgaacaac gcgccgagca ggctgccgcc
     5401 cacgctcagc gcggacgaca tggtctcgtg gctggcctgg ctcctctcgc gggcgacctt
     5461 ctgttccgcg cggcgctggc ggtcagtgag cgtcgccagc cggctcgcgt acttgcggcg
     5521 caggtcgtcg acggcccggt cgcgtttctc gcgcaggctc tgcgcgagct gcgcgcgaaa
     5581 ctcgccttcc gtctgctgcg gcgtcgccgc ggccttgagc tcgggacaac gcaggacggc
     5641 gagcgtactc gtgcggtaga cgtgctccac cagcgcccgg gcgtgctgct gcagggcctt
     5701 cggcgcggcc agcacgccgc ccgggaccgg cgcgaaggcg gcgtctggcg agggctcgtc
     5761 gcggaggtcg agcgcgtcgc gatcgcagga ttcggctcgt tcccaggccg gaccgcgctc
     5821 gtcgagcggc gccaggcgat aggtgtccgt ccacaggtcg acgccgaggc gggcgtcgac
     5881 gaagtgcgtg cgcacgcgcg cgccgagcct cggctcatac tcgacggccg tgcctgaggc
     5941 cgccaggaac tgctccggca ggccggccgg caccaccggg cgactcgacg aggtggccgc
     6001 cggcttcggc gacggggccg gggcaggcac gccggcctcg acgcgtgcga cggccggtga
     6061 aggtgccccg cccgccgcgc cgagacgcgt tatctcggcg agcgtcagcg ggcctcgcag
     6121 gtacgacagg gcccagcgcg tgcgcagcag caccggcgcg tcgtcgtgga cgttgcgcat
     6181 caggaaggtg cgttgcggca ggttcgacat tgccgcctcg agttgcgtct tgtccatgcc
     6241 ggccgcggcg ttgctgccga gcagcccttc gatcacgcgc agcttgtccc gctcggtctg
     6301 caggcgcccg atgaaccagg tccccgcgtt gccgaggccc ttgtaatcca ggtccaccgg
     6361 attctgcgtc gccaacacga cgccgacgcc gaacgcacgc gcctgcttca tcagcgtgag
     6421 cagcggtgtc ttcgacggtg gcatcgcgct cggcgggaag tagccgaaca cctcgtccat
     6481 gtagaacagc gcgcgcaggc tcgtggtgcc gggctggcgc cgcatccacg ccacgagctc
     6541 gttggcgatc agcgtgacga cgaacatgcg ttcggcgtcg ttcaggtgcg cgatcgagac
     6601 gatcgcgatg cgcggccggc cgtcggcgtc gtagagcatc gaggggacgt cgagcgggtc
     6661 gccctgcaac cacgcctcga accccgggga ggcgagcagc gcgttgatcc gcagcgcgag
     6721 ctcggtgcgg tccttcgccg gatagaagct ttcgaggtcg aacacgccga cctgctcgaa
     6781 cggcggtttc tgcaccgacc ggatcagcgc ggcgaggtcg aggctctcac ccttgcgcca
     6841 ggccgcgtcg agcagcgcgc acagcaggat gtactcgcgg ctgcggatcg ggtcggcctc
     6901 gatgtcgagc aggccgagca atccggccac ggccgagccg acccgctcct tgagcgccgt
     6961 cgcatcgccc gccacggcgg cgtcgggtgc cgcgaacgag cgcaggatcg acaggggccg
     7021 tccggcactc gaccctggcg tgtaaacggt cacggccgcc gcgtcgcgca gtcgctggat
     7081 tcgcgtgccg tcctggcccc actcctcgag ccccctgcgc cacgttgccg cggtcgccgc
     7141 ggcgaaatcg tccaggctct ggcccttgcg cgccgcctcg ccctgatcga cccatggctt
     7201 gaaatcctcg gcccgcagtt gcggaaacgt gagcgcgagg ttcgcgatgt cgcccttggg
     7261 gtcgatcgca atcgcgggga tgccgtcgat cgcggcctcc tcgagcagcg acaggcacag
     7321 gcccgtcttg ccgctgccgg tcatgccgat gcagacggcg tgcgtggtca ggtcgcgcgc
     7381 gtcgtagagg agctcgggac cgacctggcc cgtggcctgg tcgtactcgc gcccgaggta
     7441 gaagacgccg agcttttcgt agttgggcag ggccatgggt cgtccgatga atggcgcgtc
     7501 gtgccgacgg cgcttgtttt gagggcatcg taaaccaacc tgccggatcg gtcccggcac
     7561 cccgggcgcc cgccgtgcag gaagccgccg acgccagctt cagacgccgc gccaaggtcc
     7621 gcctcgcttg tcaccggttt gtcacccgcg ggcgactgac gccgtcaccg cgcctcccta
     7681 cgatgccgcg aaccctccgg atccgcgtgt cgcgcatgtc catcgccctc gaccaggtca
     7741 ccaagcggta ccaaggcgta ccggtcgtca acgacgtgtc gctgagcgtc cggccgggcg
     7801 agttcctggt gctgctcggg ccgagcggca gcggcaagag cacgctgctg cgcgcgatcg
     7861 cggggctcac cgacatcgat cacggccgca tctcgctgca cggcaaggac gtcacgcacc
     7921 tcacggcgcg ccagcgccag gtcgggttcg tcttccagca ctacgcgctg ttccggcaca
     7981 tgacgatcgg cgacaacatc gaattcgcgc tgcgcgtacg caaggtccgg gccgccgagc
     8041 gccgggcacg ccgcaaggag ctgctcaagc tcgtcgccct cgaaggcatg gacgaccggc
     8101 tgccggcgca gctctcgggc ggccagcagc aacgcgtcgc cgtggcccgc gcgctcgcgc
     8161 accggccgga agtgctgctg atggacgagc cgttcggcgc gctcgacgcg aagatccgcg
     8221 aggaactgcg ccgcacgatc cggcagatcc agcgcgaact cgagatcacg acgatcctcg
     8281 tcacgcacga ccaggaagag gctttcgcgc tggctgaccg catcggcgtc atgcaccagg
     8341 gccgcctgct cgagtgcggg cggccggacg acctctacac gaggcctgcg acgcgcttcg
     8401 tgtcgacgtt cctgggcgcg gcgaacctgc tgctcgggta ccgcacctcg cgcggcgtgc
     8461 gcttccacgc gcgcggcatc gagcggccgc acgaagtggt ggccgtgctg cggcccgagg
     8521 aggtcgaact cgcggccgac gagtccggga tccgggcgaa tttcgtgggc cacggcgtcc
     8581 tcgaggaggt gctgttcggc ggtgccgtgg agcgactgcg cgtgcgcatg gcgagcgacg
     8641 gtcccgtgcc cgcgggcagc aacgagagtc atcaacgccg ggggttgctg ctcgaggtgt
     8701 cgcgcaccct gcccgagcag cgcgatttcc cagtgagcca cgggctgcgc gtagcggtcg
     8761 gcgcgcgccg catccacgtg ctgcccacgc cgatctcgag cttcacggcg gtgggcgccg
     8821 acgaggcgtc cgctcgcgca atcgcggatt cgccgttgct cgcgacgctc ggtgcgcgca
     8881 tgcagacgcg ggtcggccag caggtcggca cggcgcaacg cgcgccgccc ggcatgcccg
     8941 tcctcgcgac cggccccggc agtgccgaga cggcccgctg gcagctccgc aacggcgcca
     9001 cgcagctgct ctgcatcccc cccaccgccc cgctgccgca ccacgtgctg atccatacgc
     9061 tggatgcgaa ttcgcgtcgc gccacgctcg ccgtggcggc gagcctgctg cgtcacatcg
     9121 cggcggaagc cgtgtacctc gtcatccacc ccactccgtc gctcgaggcc gagcgcgccg
     9181 cccacatgcg cgacctgctc gacgcccgct cgagcgcgct caacgatcac ggcctggaca
     9241 tgcgcaccga gatgcgtccc ggagaggtga ccgaagagct gcagcgtgaa ctggcggcct
     9301 acgaggacag catgctcgtg ctcggcactt ccaatcctga ggcgctgcgg tgggactggc
     9361 tggcgtccgt gctcgagggc gagccggcgc gcccggtgct gatcgtcagc tccgcgcggg
     9421 tcgaagcgcc cgtggaggcc tgacgcgtgg ccacgtcgtg gttccgccag ccgagcatcc
     9481 tgcccggatt cggcctcacg ctcggcttca cgacgttctt cctgagcgcg ctcgtgctgc
     9541 tgccgctcgc ggcactgatc ttcaagacca ctgcgatgac gtggtcggag ttcgtctcga
     9601 cggtgctcga cgagcgggcc gtcgcgtcct accggctgag tttcggcgcg gcgctggtgg
     9661 ccgcgctcgt caacgcgttc ttcggcttca tcgtcgcctg gtcgctcgtg cggtacgagt
     9721 ttccgggacg gcgcctcgtc gacgcgctga tcgacctgcc gttcgcgctg ccgacggcgg
     9781 tctccggcat tgcgctggcg gcggtgttct cgccgaccgg ctggctcggc gtgcgcctgc
     9841 aggagtggtt cggcgtgcag gtggcctaca cgtggctcgg catcgtcgtc gcgctcacgc
     9901 tgatcgggct gccgttcgtc gtccgctcgg tgcagccggc gctgatcgag gcgcagcgcg
     9961 acctcgagga ggccgccgag acgctcggtg ccgggccgtt ctacgtcttc cgccgcatcg
    10021 tgctgccggc ggtgctgccg gccctcgtca cgggcttcac gatggctttt gcgcggggcg
    10081 tcggcgagta cggttccgtc gtgttcatct cgggcaacct gccgctcaag accgagatca
    10141 cgccgctgct gatcgtgatc aagctcgagc agttcgacta caacggcgcc gcggcgctcg
    10201 gcttctcgat gctgctcgtg tcgttcctga tgctgttcac gatcaacctc gcccaggcct
    10261 ggagccgacg gcgccacgtc ggggcagggc gctgacgtgg ccggcgcggc ccctcgctac
    10321 gaaaaacccc gcagcgtgcc cgcccccgtg cgtgccatgc gcgcgggcct cgtcttcgtc
    10381 acgatcgcgt tcctgtcgct gctgctgctc gcgccgctcg gcgcggtgct cgcgcaggcc
    10441 tttgcggagg gactgcgggt ctatctcgag tcgttcgaga accccgacac gctg
//
LOCUS       DASPFR010000162         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_169657, whole genome shotgun sequence.
ACCESSION   DASPFR010000162 DASPFR010000000
VERSION     DASPFR010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169657"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VD737_04075"
     CDS             complement(<1..312)
                     /locus_tag="VD737_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09767.1"
                     /translation="MNRIPVRRAYRYALLAAWMALVAGCGGGAETVANPATSGGTPPS
                     YSGPAPATADVQAFKINVWDNLKASNRCGQCHVSGGQAPQFVRQDDVNLAYAAANGIV
                     NL"
     gene            complement(323..1513)
                     /locus_tag="VD737_04080"
     CDS             complement(323..1513)
                     /locus_tag="VD737_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09768.1"
                     /translation="MPRTSPSTARAATARRACACVAALFVVLVPFDAVADARSQAKRI
                     HDRLAGVPPTEAVLTSMASDVAAGRAVDAAQTAMQHRAFYDVTLKNFAMPWTNRDQTV
                     FAPLNDYVATVIGMVRDDVAFDRVLYDDILYVGRSGLGVPGVSASSNDHFAALEAQHV
                     NLKDDLVQATQSSLYGLPAEATAGVMTSRAAAQAFFIAGTNRAMFRYTLLNHLCTDLE
                     QVQDPTRPPDRIRQDVSRSPGGDSRVFLNNCIACHSGMDPMAQAFAYYDYDEAGGRML
                     YTAGMVRPKYFNNEETFPPGFSTPDDAWDNYWRQGRNSLLGWDGSLPGRGSGAKSLGR
                     ELARSDAFATCQVQKVFRAVCLRPPSDAADRARVQAMAGDFKAGGYRLKQVFAAAATY
                     CMGD"
     gene            1729..>2187
                     /locus_tag="VD737_04085"
     CDS             1729..>2187
                     /locus_tag="VD737_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09769.1"
                     /translation="MNVRVASLAHRRVAAWARAFALALAFTASSISCTSDGGAGGGPA
                     GGQSPDPVVLDFPVAYVKRAVPLDDAMQQDARNLLDFQPGGDLYLRDRASPSAPERNL
                     TSAVTLGEGDVRDVEPSFDGRKLVFALHEPMIEGASPDEQPTWDIWEFDLD"
BASE COUNT          324 a          751 c          764 g          348 t
ORIGIN      
        1 gaggttcacg atcccgttgg cggcggcgta cgcaaggttt acgtcgtcct gacggacgaa
       61 ctgcggcgcc tggccgccgg acacgtggca ctgtccgcag cgattcgagg ccttcaggtt
      121 gtcccagacg ttgatcttga acgcctgcac gtcggcggtg gccggcgccg gccccgagta
      181 cgacggcgga gtaccgcccg aggtcgcggg gttcgcgacg gtttcggcgc cgccgccgca
      241 accggcgacg agcgccatcc acgcggcgag cagcgcgtag cggtacgccc ggcgaacggg
      301 gattcggttc atcgcgagat cctcaatcgc ccatgcagta ggtcgccgcc gcggcgaaga
      361 cctgcttcag ccggtagccg ccggccttga agtcaccggc catggcctgc acgcgcgccc
      421 ggtcggcggc gtcgctcggc gggcgcaggc acacggcgcg gaagaccttc tgcacctggc
      481 aggtcgcgaa ggcgtcgctg cgcgcgagct cacggccgag cgacttggcc ccgctgcccc
      541 gacccggcag cgagccgtcc cagccgagca gcgagttgcg gccttgacgc cagtagttgt
      601 cccaggcgtc atccggtgtc gagaagccgg gcgggaacgt ttcctcgttg ttgaagtact
      661 tcggccgcac catccccgcc gtgtaaagca ttcttccacc ggcctcgtcg tagtcgtagt
      721 acgcgaaggc ctgcgccatc gggtccatgc cggagtggca ggcgatgcag ttgttcagga
      781 acacccgact gtcgccgccc gggctgcgac tcacgtcctg gcggatccgg tccggtggcc
      841 gcgtcgggtc ctgcacctgc tcgaggtccg tgcagaggtg gttcagcagg gtgtagcgga
      901 acatcgcgcg gttcgtgccc gcgatgaaga acgcctgcgc cgcggcgcgc gaggtcatga
      961 cgcccgcggt cgcttctgcc ggcagtccgt acagcgacga ctgcgtcgcc tgcaccaggt
     1021 cgtccttgag gttgacgtgc tgggcttcga gcgcggcgaa gtggtcgttg ctggaggcgg
     1081 acacgcccgg gacgcccagg ccgctgcggc cgacgtacag gatgtcgtcg tagagcacgc
     1141 ggtcaaacgc cacgtcgtcg cgcaccatgc cgatcacggt cgccacgtaa tcgttcaacg
     1201 gtgcgaagac ggtctggtca cggttcgtcc agggcatcgc gaagttcttg agcgtgacgt
     1261 cgtagaacgc ccggtgctgc atcgcggtct gggcggcgtc gacagctcgc cccgcagcga
     1321 cgtcgctcgc catcgaagtc agcacggctt cggtcggcgg cacgccggcg aggcggtcgt
     1381 ggatgcgctt cgcctgcgag cgcgcatcgg ccaccgcgtc gaacggcacc agcacgacga
     1441 acagcgccgc cacgcatgcg caggctctgc gcgccgtggc ggcccgcgcg gtcgacggtg
     1501 acgttcgagg catgccttcc tcctgcacga cggtggtccg ccgggccgcg caacgatccc
     1561 cgcgcgcagg tgaccacgcc aagcccggct tacgtttgtg agagccgcct cacacaaagc
     1621 gccttgtgag agccgcctca cacaaacgcc gcgagtttcc gaatccttgc ttgggttgcg
     1681 acaacatccg tgggcgacga ccccggtggt cgtcccgcag gcaggaacat gaacgtacgt
     1741 gtcgcatcgc tggcgcatcg gcgtgtcgca gcatgggcga gggctttcgc actggcgctg
     1801 gcgttcacgg cgtcgtcgat ttcctgtacg agcgatggcg gcgcaggcgg cgggcccgcc
     1861 ggcggtcagt cgcccgaccc ggtcgtgctc gactttccgg tcgcctacgt caagcgtgcg
     1921 gtgcccttgg acgacgcgat gcagcaggac gcgcgcaacc tgctcgactt ccagcccggc
     1981 ggcgacctgt acctgcgcga ccgcgcgtcg ccttccgcgc ccgagcgcaa cctcacgtcg
     2041 gccgtgacgc tcggggaagg cgacgtgcgg gacgtcgagc cgtcgttcga cggccgcaag
     2101 ctcgtgttcg cgctgcacga gccgatgatc gaaggtgcct cgcccgacga gcagccgacg
     2161 tgggacatct gggaattcga cctggac
//
LOCUS       DASPFR010000163         6024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_536231, whole genome shotgun sequence.
ACCESSION   DASPFR010000163 DASPFR010000000
VERSION     DASPFR010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536231"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="VD737_04090"
     CDS             complement(<1..1329)
                     /locus_tag="VD737_04090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXW09770.1"
                     /translation="MRHRKVVAILVALGALTLLALALADLPTGSKGLAIVAALLAYGL
                     LVHWVRSSLKGQEALLDALADGLWSLHDKDYSVSIAEPRDARLRRLVAGYNGLGSKLR
                     SQRQDLYQRELLLDTVIQASPLAMVLTNATGTILYGNLAARELLSRGRRLEGLALAPL
                     LETQPEALREALEDTRDQLFTTKFGDEAQVYHVSHRQFLLNGQPHRLRLLKQLTRELN
                     AQEVAVWKKVIRVIAHEINNSVAPIASLAQSGHRLAQQPEPAQLQRVFAAIDDRMQHL
                     SQFVEGYSRFAKLPKPRPAAVEWRPFLESLKAVSPFTIEDVLPSGQAWFDESQIEQVM
                     INLLKNAIEASPADASVTVSLSFSPDVRDVLFDAVGDFAIIRVRDNGLGLTEEDKQRV
                     FEPFFSKKSGGTGLGLYVTHSIIERHSGYIYVDSEYGVGTTFSVYLPVKQV"
     gene            complement(1340..2710)
                     /locus_tag="VD737_04095"
     CDS             complement(1340..2710)
                     /locus_tag="VD737_04095"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXW09771.1"
                     /translation="MHGRTLLVIDDNDAVRTALDVLLTLEGARVEQAATPREGLGRLA
                     RGGVDLVIQDMNFQREATSGREGIELFRAIRATHPDMPVVLMTAWTSLETAVELVKAG
                     AADYLAKPWDNARLVTTVRNLLQLRAALEQERERQSRLDRSREELAGRFDLRGIVYRS
                     DALHALVQTATQVARADVPVLITGPNGAGKEVVAEIIQANSSVRDGPFVKVNAGALPA
                     DLLESELFGAESGAYTGARARAGRFEAADGGTLFLDEIGNLPPTGQAKLLRVLQTGEF
                     ERLGSSQTRRVKVRILSATNTSLLEAIRQGRFREDLYYRLNVIELRVPPLAERRDDVL
                     PLARHFLEPGYELTGEAERALLRHDWPGNVRELRNCLRRASLLAGSHRIGAQDLGLPA
                     GATAGLSGESSTSLREPDRAEIEGALARHQGVVAKAARDLGLTRQSLYRRMEKLGIAA
                     GAPDGA"
     gene            complement(2799..4004)
                     /locus_tag="VD737_04100"
     CDS             complement(2799..4004)
                     /locus_tag="VD737_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXW09772.1"
                     /translation="MDFKHILSSLRRSPTGAILVALQIALALAIVVNSLFIVVQRLET
                     LNADHGLDVPNIFAVGWTVSGDRFNGEATMREDLAILRGLPGVVAATVTNALPLSGGG
                     SSNSLYTEQNEKGNRGDVNVFFVDEQAARTFGVKIAEGRDLDAAVVRKPPRNTSSFPP
                     EILLTREAAKEMFPKGSALGKTVYTSINQPATVVGIIDRMHGSWPTWDKFANVALAPE
                     IPDEQYARYLVRTEPGRRDELMRQAEDALAKVENGRVILKVRSLEYVAASTYADDRAM
                     AVYLTVVIGLLLGISALGIFGLASFNVSTRTKQIGTRRAVGARRLDILRYFLVENWLI
                     TTVGVVVGCVLALLLGYWLSTAFELPRLKLYYLVGGAVGLWLLSLAAALRPARRAALV
                     SPAIATRTV"
     gene            complement(4024..5358)
                     /locus_tag="VD737_04105"
     CDS             complement(4024..5358)
                     /locus_tag="VD737_04105"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXW09773.1"
                     /translation="MYAYDLRLAWHSIRRNPGLAALMVLAMALGIAVVTVTFTVYHAM
                     ATNPIEHKNDRLNAVTIDTWDPQQPYDEDKPEHPPTLMTYKDAMHLYASDAAPRALVM
                     YKSGVLVLPERQGVKAFNAITRVTTHEFFTMFDVPFQYGGGWDAAADRGPEPVVVLSD
                     ETNQKVFGGENSVGRTLRLNKNDYRVVGVLEPWAPSPKFYDLNNGSFDDPEDLYLPFG
                     WGRALELPVYGNTNCWKSETFNAYADFLNSECVWLQVWADLPEPADREKFQAYLDNYA
                     RSQKAAGRLPRPLNNQIYDVDAWLDRNEVVQKDNRVLIGIGVLFLGVCVVNVVGLLLA
                     KFLNGAPMTGLRRALGASRKDIVRQHMTEVLVLGVAGGVLGLLLAVGGLAGIRSFYTS
                     DWNRAAAERLTEIDPIVVLVTLALSVVAGAIAGLYPSWRIGRTAPAVYLKTQ"
     gene            complement(5381..>6024)
                     /locus_tag="VD737_04110"
     CDS             complement(5381..>6024)
                     /locus_tag="VD737_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXW09774.1"
                     /translation="FVAVTGPSGSGKTTFLNVAGLLETFDSGTYRLDGVDVSKLDDNA
                     RSQVRNRKIGFIFQSFNLIPDLNVFDNVDVPLRYRRMPTAERRERITAALDLVGLGSR
                     NKHLPSQLSGGQQQRVAIARALAGDPALLLADEPTGNLDSLMARQVMDLLEQINGMGT
                     TIVMVTHDPELARRAHRNVQVIDGQITDYKPYEPHAASVTHLHPPTAGAAAVG"
BASE COUNT          902 a         2068 c         2090 g          964 t
ORIGIN      
        1 cacctgcttc acgggtaaat agaccgagaa cgtcgtcccc acgccgtact cgctgtcgac
       61 gtagatgtag ccgctgtgac gctcgatgat gctgtgcgtc acgtacaggc ccagtccggt
      121 accgcccgac ttcttggaga agaatggctc gaagacgcgt tgcttgtctt cttccgtgag
      181 ccccaacccg ttgtcgcgca cgcggatgat agcaaaatca cccaccgcgt caaacagaac
      241 gtcgcgtacg tcgggcgaaa aagacagcga tacggtgacg ctggcgtcgg ccgggctggc
      301 ttcgatggcg ttcttgagca ggttgatcat gacctgctcg atctgcgact cgtcgaacca
      361 cgcctggccc gacggcagca cgtcctcgat cgtgaacggg ctcacggcct tcagcgactc
      421 gaggaacggc cgccattcga ccgcggcggg tcgcggcttc ggcagcttgg cgaaccgcga
      481 gtagccttcg acgaactgcg acaggtgctg catgcggtcg tcgatcgcgg cgaacacgcg
      541 ctgcagctgc gcgggttcgg gctgctgcgc gagccggtga ccggactgtg ccagtgacgc
      601 gatcggcgcg acggagttgt tgatctcgtg cgcgatcacg cggatcactt ttttccagac
      661 cgcgacttcc tgcgcgttca gctcgcgcgt cagctgcttg agcagccgca gccggtgcgg
      721 ctggccgttc agcaggaact gccggtgcga cacgtgatag acctgcgcct cgtcgccgaa
      781 cttcgtcgtg aagagctggt cgcgcgtgtc ctcgagcgcc tcgcgcaacg cctcgggttg
      841 cgtctcgagc agcggggcga gcgcgaggcc ttcgagccga cgaccgcggc tcaacagctc
      901 gcgcgcggcg aggttgccgt agaggatggt gccggtcgcg ttcgtgagca ccatcgcgag
      961 cggcgaggcc tggatcaccg tgtcgagcag cagctcgcgc tggtagaggt cctgccgctg
     1021 cgagcgcagc ttcgacccca gcccgttgta gccggcgacg agacgtcgga ggcgcgcgtc
     1081 gcgcggctcg gcgatgctga cgctgtaatc cttgtcgtgc aggctccaga ggccgtcggc
     1141 cagcgcgtcg agcagcgcct cctggccctt gagcgacgat cgcacccagt ggaccagcag
     1201 tccgtacgcc agcagcgcgg cgacgatcgc gagacccttc gaccccgtgg gcaggtcggc
     1261 gagcgcgagc gcgagcagcg tgagcgcccc gagcgcaacc aggatcgcga cgaccttgcg
     1321 atgacgcatc cgggaggcct caggcgccgt ccggcgcgcc ggccgcgatg ccgagctttt
     1381 ccatgcgacg gtacagcgac tgccgcgtga gcccgagatc gcgcgcggcc ttggcgacca
     1441 cgccctggtg ccgcgccagc gcgccttcga tctcggcgcg gtcgggttcg cgcagcgacg
     1501 tcgaggactc cccggagaga ccggccgtgg cgcccgccgg cagcccgagg tcctgtgcgc
     1561 cgatccggtg ggaccccgcg agcaggctcg cacgccgcag gcagttgcgc agttcgcgta
     1621 cgttgccggg ccagtcgtgc cgcagcagcg cgcgttcggc ctcgccggtg agctcgtagc
     1681 ccggctcgag gaaatgccgt gcgagcggca gcacgtcgtc gcgtcgctcg gcgagcggcg
     1741 gcacgcgcag ctcgatcacg ttcaagcggt agtagaggtc ctcgcggaac cggccctggc
     1801 ggatcgcctc gagcagcgac gtgttggtcg cggagaggat ccgcaccttg acgcgccgcg
     1861 tctggctcga gcccagccgc tcgaactcgc cggtctgcag cacgcgcagc agcttggcct
     1921 gcccggtcgg cggcaggttg ccgatttcgt cgaggaacag cgtgccgcca tcggccgcct
     1981 cgaaccgccc cgcgcgcgcc cgcgcccccg tgtacgcgcc cgactcggca ccgaacagtt
     2041 cgctctcgag caggtccgcc ggcagcgcac ccgcgttgac cttgacgaac gggccgtcgc
     2101 ggaccgacga gttggcctgg atgatctcgg cgacgacttc cttgcccgcg ccgttcggtc
     2161 ccgtgatcag caccggcacg tccgcgcgcg cgacctgcgt cgccgtctgc acgagcgcgt
     2221 gcagcgcgtc gctgcggtag acgatgccgc gcaggtcgaa gcgcccggcg agctcttcgc
     2281 gcgatcggtc gagccgcgac tggcgctcgc gctcctgctc gagcgccgcg cgcagctgca
     2341 gcaggttgcg gaccgtggtg accagccgcg cgttgtccca gggcttcgcg aggtagtcgg
     2401 ccgcgcccgc cttgaccagc tcgaccgccg tctcgaggct ggtccacgcc gtcatcagca
     2461 ccaccggcat gtcggggtgc gtggcgcgaa tcgcgcggaa cagctcgatg ccctcgcgcc
     2521 cggaggtcgc ctcgcgctgg aagttcatgt cctggatcac gaggtcgacg ccgccccgcg
     2581 cgagccgccc gagcccctcg cgcggggtgg ccgcctgctc gacgcgggcg ccctcgagcg
     2641 tgagcagcac gtcgagagcc gtgcgcaccg cgtcgttgtc gtcgatgacc agcagcgtcc
     2701 gaccgtgcat cgtcctctct gcgtcgacca gggcaggcgg cgccctcacc cgcgcttcgc
     2761 gcgaccgccc ccgccatcgc gggcgaggtg ccggaagctc aaaccgtgcg cgtggcgatc
     2821 gcgggcgaca ccagcgcagc ccgacgcgca gggcgcaacg ccgccgcgag gctcagcagc
     2881 cagagcccca ccgcgccgcc gaccagataa tagagcttca ggcgcgggag ttcgaacgcg
     2941 gtgctgagcc agtaaccgag aagcagcgcg agcacgcacc ccacgaccac gcccaccgtc
     3001 gtgatcagcc agttctcgac caggaaatag cgcaggatgt cgagccggcg cgcgccgacc
     3061 gcgcgccgcg tgccgatctg cttggtgcgc gtgctgacgt tgaacgacgc gagcccgaag
     3121 atgccgagcg cggagatgcc gagcagcagt ccgatcacca cggtcaggta gaccgccatg
     3181 gcgcggtcgt ccgcgtaggt ggaggcggcg acgtactcga gcgatcgcac cttgaggatc
     3241 acgcgcccgt tctcgacctt ggcgagcgcg tcctcggcct ggcgcatgag ctcgtcgcgg
     3301 cgaccgggct cggtccgcac gaggtagcgc gcgtactgct cgtccgggat ctccggcgcg
     3361 agcgcgacgt tggcgaactt gtcccaggtc ggccacgagc cgtgcatgcg gtcgatgatg
     3421 cccaccacgg tcgccggctg gttgatcgag gtatagacgg tcttgccgag tgcggagccc
     3481 ttcgggaaca tctccttggc cgcttcgcgc gtcagcagga tctcgggcgg aaacgacgac
     3541 gtgttgcgtg gcggcttgcg gacgaccgcg gcgtcgaggt cgcggccttc cgcaatcttg
     3601 acaccgaagg tgcgcgcggc ctgttcgtcg acgaagaaga cgttgacgtc gccgcggttg
     3661 cccttctcgt tctgctcggt gtacagagag ttgctcgagc cgccgcccga cagcggcagg
     3721 gcgttggtga cggtcgccgc caccacgccg ggcagcccgc gcaggatcgc gaggtcctcg
     3781 cgcatcgtgg cctcgccgtt gaagcggtcg ccgctcaccg tccagccgac ggcgaagatg
     3841 ttcggcacgt cgaggccgtg atccgcgttg agcgtctcga ggcgctgcac gacgatgaac
     3901 agcgagttca cgacgatcgc gagcgccagc gcgatctgca gcgcgaccag gatcgcgccc
     3961 gtggggctgc gacgcagcga gctcaggatg tgcttgaagt ccatggtggg cgtgctccgt
     4021 ggatcactgg gtcttgaggt acacggccgg ggccgtccgg ccgatccgcc acgaggggta
     4081 cagaccggcg atcgcgccgg cgacgaccga cagggcgagc gtcacgagca cgacgatcgg
     4141 gtcgatctcg gtcagccgct cggctgcggc gcggttccag tcggacgtgt agaacgagcg
     4201 gatgccggcg agccccccca ccgcgagcag caggcccagc acgccgcctg cgactcccag
     4261 caccagcacc tcggtcatgt gctggcgaac gatgtccttg cggctcgcgc ccagcgcacg
     4321 gcgcagcccg gtcatgggcg ccccgttcag gaacttggcg agcagcaatc cgaccacgtt
     4381 gaccacgcac acgccgagga acagcacgcc gatgccgatc agcacgcggt tgtccttctg
     4441 cacgacctcg ttgcggtcga gccaggcgtc gacgtcgtag atctggttgt tgagcggcct
     4501 gggcagtcgc cccgccgcct tctgcgagcg agcgtagttg tccaggtacg cctggaattt
     4561 ctcccgatcc gccggctccg gcaggtccgc ccacacctgc agccagacgc actcggagtt
     4621 caggaagtcg gcgtaggcat tgaacgtctc gctcttccag cagttggtgt tgccgtagac
     4681 gggcagttcc agcgcgcgcc cccagccgaa cggcaggtag aggtcttcgg gatcgtcgaa
     4741 cgagccgttg ttgagatcgt agaacttggg gctcggcgcc cacggctcca gcacgccgac
     4801 cacgcggtag tcgttcttgt tgagccgcaa cgtacggccc acgctgttct cgccgccgaa
     4861 cactttctgg ttggtctcgt cgctcagcac caccaccggc tccggcccgc ggtcggccgc
     4921 ggcgtcccag ccgccgccat actggaacgg tacgtcgaac atcgtgaaga actcgtgcgt
     4981 ggtgacgcgc gtgatcgcgt tgaacgcctt gacgccctgg cgttccggca gcacgagcac
     5041 gcccgacttg tacatgacca gggcgcgcgg tgcggcgtcc gaggcgtaga ggtgcatcgc
     5101 atccttgtag gtcatcagcg tcggcggatg ttccggcttg tcctcgtcgt agggctgctg
     5161 cgggtcccag gtgtcgatcg tcacggcgtt caggcgatcg ttcttgtgct cgatcgggtt
     5221 ggtcgccatc gcgtggtaga ccgtgaaggt caccgtgacc accgcgatgc cgagcgccat
     5281 ggccagcacc atcagggctg caaggccggg gttgcgtcgg atgctgtgcc aggcaagtcg
     5341 caggtcgtag gcgtacatgg tcgtcccctg cgtgggcgga tcagccgacg gccgccgcac
     5401 cggccgtcgg cgggtggagg tgcgtgaccg acgccgcgtg cggctcgtac ggcttgtagt
     5461 cggtgatctg cccgtcgatc acctgcacgt tgcgatgcgc gcgtcgggcg agctccgggt
     5521 cgtgcgtgac catcacgatc gtcgtgccca tgccgttgat ctgctcgagc aggtccatga
     5581 cctggcgcgc catcagcgag tccaggttgc cagtcggttc gtcggcgagc agcagcgccg
     5641 gatcgccggc cagtgcgcgc gcgatcgcga cccgctgctg ctgcccgccc gacagttgcg
     5701 acggcaggtg cttgttgcgc gagcccaggc ccaccaggtc cagtgccgcg gtgatgcgct
     5761 cgcgccgctc cgccgtcggc atgcgccggt agcgcagcgg cacgtctacg ttgtcgaaca
     5821 cgttcaggtc ggggatcagg ttgaagctct ggaagatgaa cccgatcttg cggttgcgta
     5881 cctgcgagcg cgcgttgtcg tcgagcttgc tgacgtcgac gccgtcgagc cgataggtgc
     5941 cgctgtcgaa cgtctcgagc aaccccgcga cgttcaagaa ggtggtcttg ccggagcccg
     6001 acggcccggt gaccgccacg aact
//
LOCUS       DASPFR010000164         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_902237, whole genome shotgun sequence.
ACCESSION   DASPFR010000164 DASPFR010000000
VERSION     DASPFR010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902237"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1251)
                     /locus_tag="VD737_04115"
     CDS             complement(91..1251)
                     /locus_tag="VD737_04115"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HXW09775.1"
                     /translation="MSISIRAGLVGLLAATALVGCGKKQEAPPAADTAATPAAQAPAS
                     SEEKVVNVYNWSDYIDPKTLEQFTAETGIKVNYDVFDSNEVLETKLLAGNTGYDVVVP
                     SASFMERQIKAGVFRKLDRSKITNWDKLDAEVLQRVALHDPGNEHSVNFMWGTTGIGY
                     NEGKVKAIDANAPVDSWRLVLDPKEAAKYKDCGISVLDAPSEITAVALAYLGKDPNSQ
                     SEADLKLAEDLLMKVRPYIRMIHSSNYIDALANGEICIAIGWSGDVLQARDRAAEAGQ
                     GNVIKYNVPKEGTIIWFDMFAIPADAPHPDNAHAFIDFMNRPEIAATNSNFINYANGN
                     SASFEQINEEVRTDPGIYPPEDVKAKLFPDLAESAEFTRLLNRTWTRFTTGK"
     gene            complement(1598..>2211)
                     /locus_tag="VD737_04120"
     CDS             complement(1598..>2211)
                     /locus_tag="VD737_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXW09776.1"
                     /translation="VADEVICGFGRTGRWFGSEYFGIRPDLMPIAKGLSSGYLPIGGV
                     MVSDKVADVMVERGGEFAHGFTYSGHPVACAVASANLGLLRDEKLVDRTREETGPYLA
                     GKWRELAQHPLVGEARSVGLIGALELVRDKATRTFFDNRGEVGTICRDFCFGNGLIMR
                     ATRDTMIISPPLVITREQIDELAEKAWRCLDLTYARCRDEGRL"
BASE COUNT          339 a          719 c          743 g          410 t
ORIGIN      
        1 ctcagcgcac cgacgttgag aagccagccc ccgaaacgac gcgggcggcc gacaaggccg
       61 cccgcggtgc aggaacgtcg ctcgtgcgcg tcacttgccg gtggtgaagc gggtccaggt
      121 gcggttcagc aggcgcgtga actctgccga ttcggcgagg tcggggaaca gcttcgcctt
      181 gacgtcttcc ggcggataga tgcccggatc cgtgcggact tcctcgttga tctgctcgaa
      241 cgaggccgag ttgccgttgg cgtagttgat gaagttcgag ttggtcgcgg cgatctccgg
      301 ccggttcatg aagtcgatga acgcgtgcgc gttgtccgga tgcggcgcgt cggccgggat
      361 cgcgaacatg tcgaaccaga tgatcgtgcc ttccttggga acgttgtact tgatcacgtt
      421 gccctggccc gcctcggcgg cacggtcgcg cgcctgcagg acgtcgccgc tccagccgat
      481 cgcgatgcag atttcgccgt tggcgagcgc gtcgatgtag ttggagctgt ggatcatgcg
      541 gatgtacggc cggaccttca tcagcaggtc ctccgccagc ttcaggtcgg cctctgactg
      601 gctgttggga tccttgccca ggtaggccag cgcgaccgcg gtgatctcgc tcggtgcgtc
      661 gagcaccgag attccgcagt ccttgtactt ggcggcttcc ttggggtcga gcacgaggcg
      721 ccagctgtcg accggcgcgt tcgcgtcgat ggccttgacc ttgccctcgt tgtagccgat
      781 gcccgtggtg ccccacatga agttgacgga gtgctcgttg cccggatcgt gcagtgcgac
      841 gcgctgcagg acctcggcgt cgagcttgtc ccagttcgtg atcttgctgc ggtcgagctt
      901 gcggaacacg cccgccttga tctgccgctc catgaacgag gccgacggca cgacgacgtc
      961 atagcccgtg ttgccggcga ggagcttggt ttcgagcacc tcgttcgagt cgaacacgtc
     1021 gtagttgacc ttgatgcccg tctcggccgt gaactgctcg agcgtcttcg ggtcgatgta
     1081 gtccgaccag ttgtagacgt ttacgacctt ttcctcggac gaggccggcg cctgggccgc
     1141 aggcgtggca gccgtgtcgg cggccggcgg cgcttcctgc ttcttgccgc agcccacgag
     1201 cgcggtggcc gcgagcaggc ccacgagccc ggcgcgaatg ctgatgctca tcgtatgcat
     1261 acccctgtga tgtcgatgac gatcgatccc cgtcggcgca gccgaccggc gagtgtcgtt
     1321 gtatagcgaa gaaacattgc gatggctagt ggccctgaaa tggggacact ccgaattaac
     1381 ggccaattgg ggacactcct gattttcgaa gtcctgcggg aaaagcgggt gtcccccgcg
     1441 agcgcgcaag ccgcggcagg aaccccggcc gtcgcgcggc cggttggtcc ggaccgcccc
     1501 cggcgatcgt cctggaccgt tcatgcgtcg gcaccgcagg gcacgcgggc gttaattcgg
     1561 agtgtcccca attggccgga aattcggagt gtccccctca gaggcggccc tcgtcgcggc
     1621 agcgggcgta ggtgaggtcc aggcagcgcc aggccttctc ggccagctcg tcgatctgtt
     1681 cacgcgtgat cacgagcggc ggcgagatga tcatcgtgtc gcgggtcgcg cgcatgatca
     1741 gcccgttgcc gaaacagaag tcccggcaga tcgtcccgac ctcgccgcgg ttgtcgaaga
     1801 acgtgcgcgt ggctttgtcc cgcacgagct cgagtgcgcc gatcaggcct acggatcggg
     1861 cctcgccgac cagcgggtgc tgcgccagct cgcgccactt gcccgcaagg taagggccgg
     1921 tctcctcgcg ggtgcgatcg acgagctttt cgtcgcgcag cagccccagg ttggcgctcg
     1981 cgaccgcgca cgcgaccggg tgccccgagt aggtgaaccc gtgcgcgaac tcgccgccgc
     2041 gctcgaccat cacgtccgcc accttgtccg agaccatcac gccgccgatc ggcaggtagc
     2101 cggacgacag ccccttcgcg atcggcatca gatcggggcg gatgccgaag tactccgagc
     2161 cgaaccagcg accggtccgc ccgaagccgc agatcacttc gtccgcgacc a
//
LOCUS       DASPFR010000165        11421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1514827, whole genome shotgun sequence.
ACCESSION   DASPFR010000165 DASPFR010000000
VERSION     DASPFR010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11421
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514827"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1733)
                     /locus_tag="VD737_04125"
     CDS             complement(171..1733)
                     /locus_tag="VD737_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXW09777.1"
                     /translation="MSSERWRPAAAWLIVLYTQLAWGSAEPTAPTPASPVAPTPAPAV
                     EMRLPVAPAATETGPAPQAPETLSGSDAFAEFRGAFDAGRYAAAVPFAQRVLQLAEAD
                     ARTPVDEEVQVALMNLAMTQYLSGDYVGAESSYARAIELVEAAGRPLHARLARAYAGL
                     AAAYHETNRHELAVQNFQQAVALTRRHEGLLTEAQVPLLEKYVDSLTELGRYPEALQG
                     WKYVMRIATRKYGDNSPALAPTLERIGRWYTQVGLYEQARRTLRRAIDLVEAAEGARS
                     VKLIGPLTALAACNRRQLIDPKQNLFSSPDAERATMYHEPGLGSAASVSSTALMSEGE
                     RALLRAAEIAEARAEASPVQVADVRTQVGDWYQSRGLAERALPNYQKAWAAASKVKQQ
                     HEGRPIVEALFGQPVLLQIVRPDGWDKFASRSPEEVETRSVVVELTVNTEGRAEQVRV
                     IDDSGDERRAQKTTEALETARYRPRMENGAPVATAGISFTQLWILPLQRDDDPRPQGS
                     KEDGTAKSDAAT"
     gene            complement(1816..3945)
                     /locus_tag="VD737_04130"
     CDS             complement(1816..3945)
                     /locus_tag="VD737_04130"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HXW09778.1"
                     /translation="MPPTLESRHLRPPRPSELPFRSIYRHGFVRVAVGTPRVEVASPA
                     ANVAATLEVAREAAAEQAVFGVFPELGLSAYSNEDLFQQDALLDASLAALRTLVEASQ
                     DVSLTFAVGLPLIVDGRLFNCAVLLRRGRLLGAVPKTYLPNYREFYEKRQFAPAHQAV
                     STSITLLGQAVPFGTRLLFEATNLPGFVLHMEVCEDVWAPLPPSTLASLAGATVVANL
                     SASDVTVGKADYRRLLCASQSAKCVTAYLYSAAGPGESTTDLAWDGHALIYENGERLA
                     ESNRFPLMGGCILADVDLDHLRQERMRWTTFGDCVQAHRAQLQDFRRVEFELDVPADR
                     VSLRRRIERFPYVPSDPARRDERCAEVYDIQVDGLRKRLAATGIKRVVLGISGGLDST
                     QAALVAVRAFDRLGLPRRDILGYTLPGFGTSRQTFDNAHALMRTLGITAGEIDIRPSA
                     ELMLQHIGHPYAQGEPVHDVTFENVQAGERTSHLFRLANLKDALVLGTGDLSELALGF
                     TTYGVGDHMSHYNVNASVPKTLIRHLIRWLVATRQFDAATLEVLTRIADTPISPELVP
                     GAGDKPEQSSEATVGPYELQDFHLYYISRFGYRPSKVAFLAEAAWGDVRTGAWPDTVP
                     PADRHEYDLATICQWLEVFLRRFFQTSQFKRSAMPNGPKVGSGGSLSPRSDWRAPSDS
                     PATVWVEELHANVPASLRPRPRGTRRR"
     gene            4073..4450
                     /locus_tag="VD737_04135"
     CDS             4073..4450
                     /locus_tag="VD737_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09779.1"
                     /translation="MSATFFARAKGILGLPTPAAEPAKAAVVAKKPAAHHAVSIAPGP
                     RCCAGARELRGQRFLSREAPMLPLASCDRTDCTCRYEHHQDRRKGARRARDMGVAVDG
                     WIEKDHRVGEKRGRRKSDQRSDQ"
     gene            complement(4526..5176)
                     /locus_tag="VD737_04140"
     CDS             complement(4526..5176)
                     /locus_tag="VD737_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXW09780.1"
                     /translation="MQAVIVPVTAFAQNCSVVWCPRTLEAAVIDPGGDLDKVLAEVSK
                     RGLKLTKILLTHAHIDHAGATAELARRESLPIVGPHRGDKFWIDGLPEQGRMFGFGPC
                     ETFEPDQWLDEGDRVKVGDVEFEVHHCPGHTPGHVVFHSVPDKLAFVGDVLFQGSIGR
                     TDFPGGDYATLISAIRDRLFPLGDDVRFVPGHGPMSTFGQERRTNPFVGDAAVGKG"
     gene            complement(5178..5804)
                     /locus_tag="VD737_04145"
     CDS             complement(5178..5804)
                     /locus_tag="VD737_04145"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HXW09781.1"
                     /translation="MRKIWNTVLKGLVAILPLGLTVYVVYWLAVSAERLFSRLIKLVV
                     PEPYYWPGLGLLAGVVLLYFTGLAVNAYVVSRALRVSDRLFARIPVVKTIYLAIRDFT
                     RFFPSSGQASDLRRVVLVPFGPGKVIGFVTAESGEMLRRSASADDAVAVYLPMSYMVG
                     GYTVFLARELVEPTSLTVEEGMRIALMGGVRGGGPGPGAGTGVVEEAR"
     gene            5862..7331
                     /gene="dacB"
                     /locus_tag="VD737_04150"
     CDS             5862..7331
                     /gene="dacB"
                     /locus_tag="VD737_04150"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HXW09782.1"
                     /translation="MPRSTIHRAASPRISSLLLVAALAAGASGCAHARDKPYLPPEVE
                     DVVKRRKVPPASLSVYVREVGREEPLVSFNSDVPRNPASTMKLLTTYAALETLGPAYT
                     WRTRAYAAGAVRDQVLEGDLVLVGGGDPFMIADRWWGFVNGLRQAGVQRIAGDVVIDN
                     SYFAPQGDDRAAFDNRPYNSYNVLPDALLVNFQTVTINAVPDVATGTVRASVNPWPAN
                     FAIDSNVRLDPGPCRRGSGGISVAMPEGAMGARVVLGGRYAAGCGQFSTSRAVMKPPD
                     YAFGLFKSFWQQAGGTIGGGLRLGPLPPDAKLLYTHDSLTLAEVIRLINKYSSNAMAR
                     SVYLTLAAERYPDRPATTDASREAIDDFLASREIAIPALVIENGSGLSRNERITAAGL
                     ADVLLAAWQSPYMPEFAASLPLSATDGTLKRRFRAPEMQGRLRMKTGSLTDVAALAGY
                     VSAASGKTFVTVVMLNHPAASQGAGEALQTALVEWVFGQ"
     gene            7420..7839
                     /locus_tag="VD737_04155"
     CDS             7420..7839
                     /locus_tag="VD737_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004278705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HXW09783.1"
                     /translation="MAYDRNNVFARILRGEIPAHKVCEDERTLAFMDVMPQADGHTLV
                     IPKHAAENLFDLPADDLAATIRTTQRVARAVRKAFDAPGIMIAQLNGAAAGQSVFHIH
                     FHVVPRHAGIDLRFHARDMADPARLAEHAERVRAALD"
     gene            7893..9836
                     /gene="acs"
                     /locus_tag="VD737_04160"
     CDS             7893..9836
                     /gene="acs"
                     /locus_tag="VD737_04160"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HXW09784.1"
                     /translation="MHKALYEVPAEFAARARLRKDDYEKLYAESVADPEGFWSRMGRR
                     IDWVQPYTKVRDVSFDASDFRIRWFYDGKLNVAWNCLDRHLAERGDKVALIHEGDDPD
                     ESAKVTYRELYHRVCKLANALKSLGVGKGDRVTIYLPMIPEAAVAMLACARIGAIHSV
                     VFGGFSPDSLAGRIADCASTVVITADEGVRGGKKVPLKANVDQALSSPGTGTVKHVLV
                     VKRTGASVPMYQRDRWYSELVDAQPDECPCEPMDAEDPLFILYTSGSTGKPKGVLHTT
                     GGYLVYAALTHEAVFDLREDDVYWCTADVGWVTGHSYVVYGPLANGATSVVFEGVPNF
                     PDPGRFWQVCDKHAVTIFYTAPTALRALMREGEGPVKKHSRATLRLLGTVGEPINPEA
                     WEWYHRVVGDGRCPIVDTWWQTETGGILISPLPGAIAQKPGSATRPFFGVKPAIVDND
                     GHVLEGACEGNLVLIDSWPGQMRTVFGDHQRFIDTYFRTFPGRYFTGDGAKRDADGYY
                     WITGRVDDVLNVAGHRLGTAEIESALVAHHLVAEAAVVGCPHDIKGQGIYAYVTLVLD
                     AEPTEQLRKELREWVRREIGPIATPDYIQWAPGLPKTRSGKIMRRILRKIAANESEQL
                     GDISTLADPSVVAQLVDNRMIRA"
     gene            complement(9942..10142)
                     /locus_tag="VD737_04165"
     CDS             complement(9942..10142)
                     /locus_tag="VD737_04165"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HXW09785.1"
                     /translation="MVDDDRVEGSAKNIGGKIKEGVGKAVGDEKLRREGQLDQVKGRA
                     ENAIGGIKDEAREQRDDIERKR"
     gene            complement(10273..10692)
                     /gene="cueR"
                     /locus_tag="VD737_04170"
     CDS             complement(10273..10692)
                     /gene="cueR"
                     /locus_tag="VD737_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010188993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HXW09786.1"
                     /translation="MPVAAVRGQHHNIGSAAQLTGVSAKMIRHYEDIGLLPRVDRTAS
                     GYRVYGEADLHRLRFVRRARSLGFSIRDIGALLGLWDDPHRASRDVKRLAHAQIEDLE
                     RRIAEMQSMQRTLRELARRCHGDTRPECPVLDDLAGR"
     gene            complement(10743..>11421)
                     /locus_tag="VD737_04175"
     CDS             complement(10743..>11421)
                     /locus_tag="VD737_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HXW09787.1"
                     /translation="ESGLDPDAGRQQRSAHEAQGRTVSWLFEDRDDGARLLGLLAFGD
                     APRPSSREAVEALHGMGVRTVMISGDNRGAAESIARMTGIAPEDVIAQVLPGDKAAVV
                     ERLRAEGPVAMVGDGVNDAPALAAADVGFAMGSGTDVAMHAAGVTLMRPDPRLVADAI
                     DVSRRTTRKIHQNLFWAFVYNVVGIPLAAAGLLNPVVAGAAMAFSSVSVVSNTLLLRR
                     WRPREAR"
BASE COUNT         1758 a         4030 c         3938 g         1695 t
ORIGIN      
        1 gagccggcag tgccagggca atcgcaacca acgcagccgc agcccgaggc gtcggagcca
       61 gagccgtcgg aaccagctcg gccgcaacct gaggggcagc agccctgacg gcgtcgtccg
      121 gcttcgacga gtcacggcca cggccgaagc cgctgacggc gaacggccgc tcaagtcgcg
      181 gcgtcggact tcgcggtgcc gtcttccttc gatccctgcg gccggggatc atcgtccctc
      241 tgcagcggca ggatccacag ttgcgtgaac gagatgccgg cggtggccac cggtgcgcca
      301 ttctccatgc gcggccgata acgcgcggtc tcgagcgcct ccgtcgtctt ctgcgcgcgt
      361 cgctcatcgc cggaatcgtc gatcaccctg acctgttccg cgcgcccttc ggtgttcacg
      421 gtcagctcga cgacgacgct gcgcgtctcg acttcctccg ggcttcgcga cgcgaacttg
      481 tcccagccgt cggggcgcac gatctgcagc agcaccggct gcccgaacag ggcttcgacg
      541 atcgggcggc cttcgtgctg ctgcttgacc ttggaggcag ccgcccacgc cttctggtag
      601 ttgggcagcg cacgctcggc caggccgcga ctctggtacc agtcgcccac ctgcgtgcgc
      661 acgtccgcca cctgcacggg cgacgcctcg gcgcgcgctt cagcgatctc cgccgcgcgc
      721 agcagcgccc gctcgccctc ggacatcaac gcggtcgagg agacggatgc cgctgaaccg
      781 aggcccggct cgtggtacat cgtcgcgcgc tcggcgtcgg gcgaggagaa caggttctgc
      841 ttcgggtcga tcagctggcg gcggttgcag gcggcgagtg ccgtcagcgg gccgatcagc
      901 ttgacggacc tggcgccctc tgctgcttcg acgagatcga tcgcgcgccg caacgtgcgt
      961 cgcgcctgtt cgtacaggcc aacctgggtg taccaccgcc cgatgcgctc cagcgtcggc
     1021 gcgagcgcgg gactgttgtc gccgtacttg cgcgtcgcga tgcgcatcac gtacttccag
     1081 ccctggaggg cctcggggta gcgcccgagc tcggtcagcg agtcgacgta cttctcgagc
     1141 agcggcacct gcgcttccgt cagcagccct tcgtggcggc gcgtgagcgc gaccgcctgc
     1201 tggaagttct ggacggcgag ctcgtgacgg ttggtctcgt gatacgcggc cgcgagcccg
     1261 gcgtaggcgc gcgcgaggcg tgcgtgcagg ggccggcccg cggcctcgac caattcgatc
     1321 gcccgcgcgt atgacgactc ggcaccgacg tagtcgccgc tcaggtactg cgtcatcgcg
     1381 aggttcatca acgcgacctg gacctcctcg tcgacgggcg tgcgggcgtc cgcctcggcg
     1441 agctgcagca cgcgctgcgc aaacggcacg gccgcggcgt acctgccggc gtcgaatgca
     1501 ccgcggaatt ccgcaaacgc atcactgccg ctcaaggtct ccggcgcctg cggcgccggc
     1561 ccggtctccg tggccgcggg cgccaccggc aagcgcatct cgacggccgg ggcgggggtc
     1621 ggcgcgaccg gggaagcggg cgtcggcgcg gtcggctcgg cgctgcccca ggcgagttgc
     1681 gtgtacagga cgatcagcca cgcggccgcg gggcgccagc gttcggagga cacgggtttc
     1741 acgtcccgag gcttacacgg cgcgcgcgat gcttgcaacc ggaccggcgg cggcgcggcg
     1801 gctcacgacg cgacgtcagc ggcgacgcgt gccacgcggc ctcgggcgca gcgaagccgg
     1861 cacattggcg tgcagttcct cgacccagac ggtggcgggc gaatcgctcg gcgcacgcca
     1921 gtcactgcgc ggtgacagcg agccgccgga gccgaccttg gggccgttcg gcatcgcgga
     1981 gcgcttgaac tggctggtct ggaagaaccg gcgcaggaag acctcgagcc actggcagat
     2041 cgtcgcgagg tcgtactcgt gccggtcggc cggagggacg gtgtccggcc acgcgcccgt
     2101 gcgaacgtcg ccccacgcgg cctcggccag gaaggcgacc ttgctgggcc ggtagccgaa
     2161 gcggctgatg taatacaggt ggaagtcctg cagttcgtac gggccgaccg tggcttcgga
     2221 ggactgctcg ggcttgtcgc cggcgccagg cacgagctcg ggcgagatcg gcgtgtcggc
     2281 gatgcgcgtc agcacctcca gggtcgcggc atcgaactgg cgcgtcgcga ccagccaccg
     2341 gatcaggtgg cggatcagcg tcttcggcac cgacgcgttg acgttgtagt gcgacatgtg
     2401 gtcgcccacg ccgtaggtcg tgaagccgag cgcgagctcg ctcaggtccc cggtgccgag
     2461 cacgagcgcg tccttgagat tggcgagccg gaacaggtgc gaggtgcgct cccccgcctg
     2521 cacgttctcg aacgtgacgt cgtgtaccgg ctcgccctgc gcgtacggat ggccgatgtg
     2581 ctgcagcatc agctcggccg acgggcggat gtcgatttcg ccggccgtga tgccgagcgt
     2641 gcgcatcagc gcgtgcgcgt tgtcgaacgt ctgccggctc gtgccgaagc cgggcagcgt
     2701 gtaaccgagg atgtcccgcc gcggcagccc gagccggtcg aacgcccgca ccgcgaccaa
     2761 tgccgcctgc gtcgaatcga gaccgccgga gatcccgagt acgacgcgct tgatgccggt
     2821 ggccgccagg cgcttgcgca gcccgtcgac ctggatgtcg tagacctcgg cgcagcgctc
     2881 gtcgcggcgg gccgggtcgg acggcacgta cgggaaacgc tcgatccgcc gccgcagcga
     2941 gacgcggtcc gccggcacgt ccagctcgaa ctcgacccgg cggaagtcct gcagctgcgc
     3001 gcgatgggcc tgcacacagt cgccgaacgt cgtccagcgc atgcgctcct ggcgcaggtg
     3061 gtcgaggtcg acgtcggcca ggatgcagcc gcccatcagc ggaaaccggt tcgattccgc
     3121 caggcgctct ccgttctcgt agatcagcgc gtggccgtcc caggcgaggt cggtcgtcga
     3181 ttcgccgggg ccggccgccg agtacaggta ggcggtcacg catttggccg actgcgacgc
     3241 gcagagcagc cgccgatagt cggccttgcc gaccgtgacg tcgctcgccg agaggttcgc
     3301 gacgacggtg gcgccggcca gcgacgccag cgtgctcggc ggcagcggcg cccagacgtc
     3361 ctcgcacacc tccatgtgca gcacgaagcc gggcaggttg gtcgcctcga acagcagccg
     3421 cgtaccgaac ggaacggcct ggccgagcag cgtgatcgac gtcgagacgg cctggtgggc
     3481 gggcgcgaac tgccgctttt cgtagaactc gcggtagttt ggcaggtagg tcttcgggac
     3541 cgcgccgagc aggcggccgc ggcgcagcaa caccgcgcag ttgaacagcc ggccgtcgac
     3601 gatcagcggc aggccgacgg cgaaggtcag cgagacgtcc tggctcgcct cgacgagcgt
     3661 gcgcagcgcc gcgagcgagg cgtcgagcag cgcgtcctgc tggaacaggt cctcgttcga
     3721 gtacgccgac aggccgagct cggggaagac accgaacacc gcctgttcgg cggccgcctc
     3781 acgggccact tcgagcgtgg ccgcgacgtt ggctgccggg gaagcgacct cgacccgagg
     3841 cgtgccgacc gccacgcgca cgaaaccgtg gcggtagatc gagcggaagg gcaactcgga
     3901 cgggcggggc gggcggaggt ggcgggattc cagggtcggc ggcatacgtt cgttctcagc
     3961 gggcagtttc gaccggacgg gcagggcggg caatagcgaa tgtgggacgc cggtcacacc
     4021 ggaatgccgt ccgctccggt tagcgtcggg tcactctgac gaggaaccgc tcgtgtccgc
     4081 cacgttcttt gctcgcgcga aagggattct tgggctgccg acgcccgcgg ccgagcccgc
     4141 caaggcggct gtcgtggcca agaagcccgc ggcccatcac gccgtgtcga tcgcgcccgg
     4201 gccgcggtgc tgcgccggcg cgcgcgaact gcgcggacag cggttcctgt cgcgcgaggc
     4261 accgatgctg ccgctcgcga gctgcgaccg cacggactgc acctgccgtt acgagcacca
     4321 ccaggaccgc cgcaagggcg cgcggcgcgc acgcgacatg ggcgtcgccg tggacggctg
     4381 gatcgagaag gaccaccggg tcggcgaaaa gcgcggccgt cgcaagagcg accagagaag
     4441 tgaccagtga atagcgaata gagacccgtc ggaaccgagg gtcggcgccc cccggatcca
     4501 ccagtcacta ttcaccggtc actgatcacc cctttcccac cgcggcgtcg ccgacgaacg
     4561 ggttcgtgcg ccgctcctgc ccgaacgtcg acatcggacc gtggccgggc acgaaccgca
     4621 cgtcgtcccc gagcggaaac aagcggtcgc ggattgcgct gatcagcgtc gcgtaatcac
     4681 cgccggggaa gtcggtgcgc ccgatcgatc cctggaacag cacgtcgccc acgaaggcca
     4741 gcttgtccgg cacggagtgg aacacgacgt gcccgggcgt gtgaccgggg cagtggtgca
     4801 cctcgaactc gacgtcgccc accttgaccc gatcgccttc gtcgagccac tggtcgggct
     4861 cgaacgtctc gcagggaccg aacccgaaca tgcgaccctg ctcgggcagc ccgtcgatcc
     4921 agaacttgtc gcccctgtgc gggcccacga tcggcagcga ctcgcgtcgc gcgagctcgg
     4981 ccgtcgcgcc ggcgtggtcg atgtgggcgt gcgtcagcag gatcttcgtg agcttgagcc
     5041 cgcgcttact gacctccgcg agcaccttgt cgaggtcgcc gcccggatcg atcacggcgg
     5101 cttccagcgt ccgcgggcac cagaccacgg agcagttctg cgcgaacgca gtgaccggca
     5161 cgatcaccgc ctgcacctca gcgcgcctct tccacgacgc ccgtgccggc gccgggcccc
     5221 ggaccgccgc cgcgcacgcc gcccatcagc gcgatgcgca tgccttcctc gaccgtgagc
     5281 gaggtcggct cgaccagttc gcgcgcgagg aacacggtgt agccgcccac catgtagctc
     5341 atcggcaggt agaccgcgac cgcgtcgtcc gccgacgccg accttcgcag catctcgccc
     5401 gactcggccg tcacgaaacc gatcaccttg cccggtccga acggcaccag cacgacgcga
     5461 cgcaggtcgc tcgcctgtcc cgaggacggg aagaaccgcg tgaagtcgcg gatcgccagg
     5521 tagatcgtct tgacgacggg aatcctcgcg aacaggcggt cggagacgcg taacgcgcgg
     5581 ctcacgacgt aggcgttgac ggcgagcccg gtgaagtaca gcagcacgac gccggccagc
     5641 aggccgaggc cgggccagta gtagggctcg ggcacgacga gcttgatcag ccgcgagaac
     5701 agtcgctccg ccgaaaccgc gagccagtag acgacgtaca ccgtgaggcc gagcggcagg
     5761 atcgccacga ggcccttgag gacggtgttc cagatcttgc gcatggagcg cgcgagtata
     5821 gccgcgcctg ccggcgcgtt cgcttatgat tcctgcagac catgccgaga tcgacgattc
     5881 atcgcgccgc gtcgccgcgc atttcctcgc tgttgctcgt cgctgcgctg gccgccggcg
     5941 cgtccggctg cgcgcacgcc cgcgacaagc cgtacctgcc gcccgaagtc gaggacgtgg
     6001 tcaagcggcg caaggttccg cccgcgagcc tgagcgtcta cgtgcgcgag gtcggtcgcg
     6061 aggagccgct ggtcagcttc aactccgacg tgccgcgcaa cccggcctcg acgatgaagc
     6121 tgctcacgac ctacgcggct ctcgaaacgc tcggtccggc gtacacctgg cgcacccgcg
     6181 cctatgcggc gggtgccgtg cgcgaccagg tgctcgaagg cgacctcgtg ctggtcgggg
     6241 gcggcgaccc gttcatgatc gccgatcgct ggtgggggtt cgtcaacggc ctgcgccagg
     6301 ccggcgtgca acgcatcgcc ggcgacgtcg tgatcgacaa ctcctacttc gccccgcagg
     6361 gcgacgatcg tgcggcgttc gacaaccggc cgtacaactc ctacaacgtg ctccccgatg
     6421 cgctgctcgt caatttccag acggtgacca tcaacgccgt gccggacgtc gcgaccggca
     6481 ccgtgcgagc gagcgtgaac ccgtggcccg ccaattttgc gatcgacagc aacgtgcgtc
     6541 tcgacccggg gccgtgtcgt cgcggctcgg gcggcatctc ggtcgcgatg cccgagggcg
     6601 ccatgggtgc gcgcgtcgtg ctcggcggac gctacgccgc cgggtgcggg cagttctcga
     6661 cttcgcgcgc cgtgatgaag ccgccggact acgcgttcgg gctgttcaag agcttctggc
     6721 agcaggccgg cggcacgatc ggcggcggcc tgcgcctggg gccgctcccg cccgacgcga
     6781 agctgctgta cacccatgat tcgctcacgc tcgccgaggt gatccggctc atcaacaagt
     6841 actcgagcaa cgcgatggcg cggtcggtgt acctgacgct cgcggccgag cgctatccgg
     6901 accgaccggc cacgacggac gcgagccgcg aggccatcga cgacttcctg gcgagccgcg
     6961 agatcgcgat ccctgccctg gtcatcgaga acggctccgg actgtcgcgc aacgaaagga
     7021 tcacggcggc cggcctcgcg gacgtgctgc tcgccgcctg gcagagcccg tacatgccgg
     7081 agttcgccgc ctcgctgccg ctttcggcca ccgacggcac gctcaagcgt cgcttccgtg
     7141 cgccggagat gcaggggcgc ctgcgcatga agaccggctc cctcaccgac gtcgcggcgc
     7201 tcgccggcta cgtgagcgcc gccagcggca agaccttcgt gaccgtggtg atgctgaacc
     7261 accccgcggc ctcccaggga gccggcgagg cgttgcagac cgcgctcgtg gagtgggtgt
     7321 tcggtcaata gactttggtc gtatgcgcgg ccggccgcgc aggggccgcc gctatactcg
     7381 tcgtcccggt cgtctccgcg accggcgacg gggccgaaca tggcgtacga caggaacaac
     7441 gttttcgcgc gaatcctgag gggcgagatc cccgcgcaca aggtctgcga ggacgagcgc
     7501 acgctcgcat tcatggacgt gatgccgcag gccgacgggc acacgctcgt gatcccgaag
     7561 cacgccgcgg agaacctgtt cgacctgccg gcggacgacc tcgcggcgac gatccgtacg
     7621 acccagcgag tcgcccgcgc cgtcaggaag gctttcgacg cgcccggcat catgatcgcc
     7681 cagctgaacg gcgccgcggc cggccagagc gtgttccaca tccatttcca cgtcgtgccg
     7741 cgtcacgccg gcatcgacct gcgtttccac gcccgcgaca tggccgatcc ggcgcggctc
     7801 gccgagcacg ccgaacgcgt gcgcgcggca ctcgactgat ttcgacgatc gacgcagccc
     7861 cgtgccggcg gggacgttag ggagacccga ccatgcacaa ggccctgtac gaagtgcccg
     7921 cggagttcgc cgctcgcgcc cgcctccgca aggacgacta cgagaagctg tacgccgagt
     7981 cggtcgccga cccggaaggc ttctggtcac gcatggggcg gcgcatcgac tgggtgcagc
     8041 cgtacacgaa ggtccgggac gtgagcttcg acgccagcga cttccgcatc cgctggttct
     8101 acgacggcaa gctcaacgtg gcgtggaact gtctcgaccg ccacctcgcc gagcgcggcg
     8161 acaaggtcgc gctgattcac gagggggacg acccggacga atcggcgaag gtcacgtacc
     8221 gcgagctcta ccaccgggtc tgcaagctcg ccaacgcact caagagcctc ggcgtcggca
     8281 agggcgatcg cgtcacgatc tacctgccga tgatcccgga ggccgcggtc gccatgctcg
     8341 cctgcgcgcg catcggcgcg atccactcgg tcgtgttcgg cggcttttca ccggactcgc
     8401 tcgccggccg catcgccgac tgcgcctcca cggtcgtgat cacggccgac gagggcgtgc
     8461 gcggcggcaa gaaggtgccg ctcaaggcca acgtcgacca ggcgctgtcg tcgccgggca
     8521 ccgggaccgt caagcacgtg ctggtcgtga agcgcacggg cgcgtccgta ccgatgtacc
     8581 agcgcgaccg ctggtattcg gaactcgtcg acgcgcagcc cgacgagtgc ccctgcgagc
     8641 cgatggacgc cgaggatccg ctgttcatcc tctacacctc gggttcgacc ggcaagccga
     8701 agggcgtgct gcacaccacc ggcggctacc tcgtctacgc ggcgctcact cacgaggccg
     8761 tcttcgacct gcgcgaggac gacgtctact ggtgcacggc cgacgtgggc tgggtcaccg
     8821 gccacagcta cgtggtctac gggccgctcg ccaacggcgc gacctcggtg gtgttcgaag
     8881 gcgtgccgaa cttcccggac ccgggccgct tctggcaggt ctgcgacaag cacgcggtca
     8941 cgatcttcta caccgcgccc acggcgctgc gcgcgctgat gcgcgagggc gaaggcccgg
     9001 tcaagaagca ttcgcgcgcg acgctgcgac tgctcggcac cgtcggcgag ccgatcaacc
     9061 ccgaggcgtg ggagtggtat caccgcgtcg tgggcgacgg gcgctgcccg atcgtcgaca
     9121 cgtggtggca gacggagacc ggcgggatcc tgatcagccc gctgccgggc gcgatcgcgc
     9181 agaagccggg ttccgcgacg cgaccgttct tcggcgtgaa gccggcgatc gtcgacaacg
     9241 acggccacgt gctcgagggt gcctgcgaag gcaacctcgt gctcatcgac agctggcccg
     9301 gtcagatgcg cacggtgttc ggcgaccacc agcgcttcat cgacacgtat ttccgcacgt
     9361 tcccgggccg ctacttcacg ggcgacggcg cgaagcgcga cgccgacggt tactactgga
     9421 tcactggtcg cgtcgacgac gtgctcaacg tcgcgggcca tcgcctcggc accgccgaga
     9481 tcgagagcgc gctggttgca caccatctgg tcgccgaggc cgccgtggtc ggctgcccgc
     9541 acgacatcaa gggccagggc atctacgcgt acgtgacgct cgtgctcgat gccgagccga
     9601 ccgagcaact ccgcaaggag ctgcgcgagt gggtgagacg tgagatcggc ccgattgcga
     9661 cgcccgacta catccagtgg gccccgggcc tgccgaagac gcgctcgggc aagatcatgc
     9721 gccgcatcct gcgcaagatc gcggccaacg agtccgagca gctcggcgac atctcgacgc
     9781 tggccgatcc gagcgtcgtg gcccagctcg tcgacaaccg catgatccgc gcctgacggc
     9841 gcggatcagt ggctagtggc tagtggctag tggctggtgg ccagtgagga ggcggctgcg
     9901 ccgcttcctt ccgactagcc actgaccact gaccactttc ttcagcgttt gcgctcgatg
     9961 tcgtcgcgct gctcgcgggc ttcgtccttg atgccgccga tggcgttctc cgcgcggccc
    10021 ttcacctgat cgagctggcc ttcacgacgc aatttctcgt cgccgacggc cttgcccaca
    10081 ccttccttga tcttgccgcc gatgttcttg gcggagcctt ccacgcgatc gtcgtctacc
    10141 atgcagtcct ccagtgctgt cttccgtgac agctcgtcct gttggaaccg atgggacacg
    10201 gagagttcac cgtgcgcttc gtgcgggatc tttccgacag gccgtcagcc cgcagcagca
    10261 ggcgcaggat gatcagcggc ccgcgaggtc gtcgagcacg gggcactccg gtcgcgtgtc
    10321 gccgtgacag cggcgcgcca gctcgcgcag cgtgcgctgc atcgactgca tctcggcgat
    10381 cctgcgctcg aggtcttcga tctgcgcgtg cgcaagacgc ttgacgtcgc gactcgcgcg
    10441 gtgcgggtcg tcccacaggc cgagcaacgc gccgatgtcc ctgatcgaga agccgagtga
    10501 ccgtgcgcgt cgcacgaagc gcaaccggtg gaggtccgcc tcgccgtaca cgcgatagcc
    10561 cgaagcggtc cggtccacgc gcggcagcaa cccgatgtct tcgtagtgcc ggatcatctt
    10621 ggccgagacg ccggtgagct gggctgcgga cccgatgttg tgatgctggc cgcggactgc
    10681 ggcgacaggc accgttcgat tcgtcttcct ggcggttcgc atgtgaatcg acctccgcag
    10741 catcaccgcg cctcgcgcgg cctccagcgt cgcagcagca acgtattgga cacgacgctc
    10801 acgctcgaga acgccatggc ggcgcccgcc accaccggat tcagcaggcc cgcggccgcg
    10861 agcgggatcc cgacgacgtt gtagacgaat gcccagaaca ggttctggtg gatcttgcgc
    10921 gtcgtccggc gcgagacgtc gatcgcgtcg gcgacgagcc tcgggtcggg gcgcatcagc
    10981 gtcacgccgg ccgcgtgcat cgcgacgtcc gtgccgctgc ccatcgcaaa gccgacgtcg
    11041 gcggcggcga gcgcaggtgc gtcgttgacg ccgtcaccga ccatcgcgac cggcccttcc
    11101 gcacgcagcc gctccacgac ggccgccttg tcgccgggca gcacctgcgc gatcacgtcc
    11161 tccggcgcga tgcccgtcat ccgcgcgatc gactccgcgg cgccgcggtt gtcgcccgag
    11221 atcatcaccg tgcgcacacc catcccatgc agggcctcga cggcctcgcg cgacgaggga
    11281 cgcggcgcat cgccgaaggc gagcagcccc agcaggcgcg cgccgtcgtc gcgatcctcg
    11341 aagagccacg acaccgtgcg cccctgtgcc tcgtgcgcgg atcgctgctg ccggccggcg
    11401 tcggggtcga gccccgactc g
//
LOCUS       DASPFR010000166         9468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_658966, whole genome shotgun sequence.
ACCESSION   DASPFR010000166 DASPFR010000000
VERSION     DASPFR010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9468
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658966"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1590)
                     /locus_tag="VD737_04180"
     CDS             complement(<1..1590)
                     /locus_tag="VD737_04180"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXW09788.1"
                     /translation="MQPAASPAPRNALANETSPYLRQHAANPVEWYPWGPEALELARS
                     LDKPILLSVGYSACHWCHVMAHESFEDPATARLMNDLFVNVKVDREERPDIDRIYQTA
                     HQVMNQAGGGWPLTVFLAPGSLRPFFSGTYFPPEQRYGMPGFRAVLEKVAEFYRTRRA
                     DLESFGDKLVEVLGQLQPPPDHAAATLGRGPLEVARATLQREFDERFGGFGDAPKFPH
                     PTNVELLLRSWRASAAGEAPDLHALYMATLTLTRMAEGGLYDQLGGGFCRYSVDAFWM
                     IPHFEKMLYDNGQLLGVYAQAAVATGEPLFRRVTGETADWLLRDLRSPEGAFYSTLDA
                     DSEGHEGKFYVWTPDEVQRVLEPEQYEAFARRFGLDQEPNFEGQWHLYVRRGVGDVAA
                     DFGLGEEAVATRLDEARARLLEVRDARVWPGRDEKILTSWNGLAIAGLARASRALARD
                     DYADAAVRAMQFLRERCWRDGRLLAVHAEGTSKYAGYLDDYASLAWGLLELLEARWEP
                     TALDWAIELAEVMLEHFTDPST"
     gene            1668..2894
                     /locus_tag="VD737_04185"
     CDS             1668..2894
                     /locus_tag="VD737_04185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019097921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HXW09789.1"
                     /translation="MSTSIAAIDHSAPALPELRLKPREERRLTAGHLWIFSNEVDTAR
                     TPLTGFAPGATCRVVSSHDRFLGYAYVNPHALICARIVGRHPEHPPGRSLIVHRLQVA
                     LALRERLYDRPYYRLVYGESDGLPGLVLDRFGDVVVGQAGTAGIEALKGEVVAAVQKV
                     LDPRTFVWKNDGGARELEGLSSYVETAFGPPVDEAIVEENDVRFVAPVGRGQKTGWFY
                     DQSANRRALLKYVPGRRVLDVFSYLGAWGLAAAKAGATEVLCVDSSAPALELLERSAR
                     ANALDHVRTERADAFDALAALRAGAEKFDVVVIDPPAFIKRRKDIPKGQAAYRKLNQL
                     AMQVLARDGILVSCSCSHHLGQDDLLHAIQQSARHLDRFAQVVEIGGHAPDHPIHPAI
                     PETRYLKALYCRVVHD"
     gene            2954..3751
                     /gene="lgt"
                     /locus_tag="VD737_04190"
     CDS             2954..3751
                     /gene="lgt"
                     /locus_tag="VD737_04190"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HXW09790.1"
                     /translation="MFVYSHPDPVALSLGPLHVRWYGLMYLVGFLAAWWLARRRASRP
                     ESSWSPGEVDDLIFYCAVGVILGGRLGWMLFYGTERLMADPLSAIRIWEGGMSFHGGL
                     VGVIAALALFASRRGRRIPDVFDFTAPLPAIGFGAGRIGNFINGELWGKPSDVPWAVV
                     VDGTPVHASQLYEAALEGLALFLILWWFTSRPRFRWAPSGLFLLCYGVFRFGVEFVRV
                     PDANRGYLFLDWVTMGQVLSLPMIVAGLAMLAYAYRQREPSGNYRAA"
     gene            3757..4551
                     /gene="thyA"
                     /locus_tag="VD737_04195"
     CDS             3757..4551
                     /gene="thyA"
                     /locus_tag="VD737_04195"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HXW09791.1"
                     /translation="MRQYQDLLRHVLERGARKTDRTGTGTLSVFGWQMRFDLEEGFPL
                     VTTKKLHLKSIVHELLWFLSGDTNVRYLREHGVTIWDEWADEEGELGPVYGAQWRAWT
                     AADGRRIDQISRAIDLIRRDPDSRRILVNAWNVGELERMALTPCHALFQFYVAEGRLS
                     CQLYQRSADVFLGVPFNIASYALLTHMVAQQCDLRPGEFVWTGGDTHLYVNHVDQART
                     QLERTPFPLPRLRIRRRPPSILDYQYDDFEFVGYQAHPAIKAPIAV"
     gene            4548..5231
                     /locus_tag="VD737_04200"
     CDS             4548..5231
                     /locus_tag="VD737_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HXW09792.1"
                     /translation="MTKRPKSAPIKPRTASRPAPGAASPHHAHMRRAAVTRSRPADEN
                     PYVVVRNSRIHGRGVYAARRIRKGTRIIEYVGDRISHDEADARYDARPDDGHTFLFVV
                     DDELCIDAGIGGNAARFINHKCDANCETIIEDRRVFIEALRTIQPGEELGYDYQLTWE
                     STDDPEELALYRCLCGAAECRGTMLDRVPLDEKRRRERARRKARKRAAVDVPGAAAKK
                     GRRAAGRAR"
     gene            5235..5747
                     /locus_tag="VD737_04205"
     CDS             5235..5747
                     /locus_tag="VD737_04205"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HXW09793.1"
                     /translation="MAALVSIVVAMDERNAIGRAGGLPWRLPDDLKRFKALTIGKPVV
                     MGRRTWDSIGKPLPGRHNVVVTRQRGLNLDGATPVGSLEQALAVVGDVPEVCVIGGAE
                     IYRVALPVAGVLHLTRVHATFDADAFFPPFEPADWVEVAREDRAADDRHGWAFSFLEL
                     RRARRNARTS"
     gene            5861..7873
                     /locus_tag="VD737_04210"
     CDS             5861..7873
                     /locus_tag="VD737_04210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXW09794.1"
                     /translation="MYDVDDDPFGPLRRWFGHWPPLLLTVAALALATLVRFALDEAWS
                     DRLPFLPYFPAILLCAMVAGWRYGALATVLSALLCVLLNVRVLDVAMLTAVALFVAAN
                     AITIMLAESARRARARAEAAAADAQERERRFEVMADSVPLIIWVHDAAGGIRFVNKAW
                     EQFFGVTQEQARRDGWQALVHDEDRAAYEGQFLECIRERKPFHAEARVRRADGAWRFI
                     ESYGVPRIGANGELVVFAGSTMDVTERHRLAVEREQLLEGERAARSEAELATRAKDEF
                     LATLSHELRTPLSVIVLWSRILARKYGGTSDELRKGLALIIDNGMALSQLIGDLLDMS
                     RIVSGRVTLDMRPIDAAEVVAQAVASHRPAADARRITLALEVGPQPKIVLGDPTRLQQ
                     ILWNLLSNALKFTHENGHIWVTARHRGRQLEIAVRDDGEGIAPEFLPQIFSRFRQADS
                     TTARRHGGLGLGLAIVKQLVELQGGEIEATSDGPGRGSTFKVTLPLHESAFATLDTDS
                     TGSWRRLDPDRLLNPRLEGLRVLAVEDQAEMLESLRQMLEEQGARVTTATSGVEALET
                     LRTHPDDFDAMVSDIGMPTMDGYELIRRVRNELGLGTDRLLAVALTAYARDEDRARAL
                     QFGFQAHLSKPYQVGQLVAIVNQLLAAQRVKNQGEAVLGEAGATAG"
     gene            complement(7881..9260)
                     /locus_tag="VD737_04215"
     CDS             complement(7881..9260)
                     /locus_tag="VD737_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXW09795.1"
                     /translation="MTPNDATILGAGLSGALMAIFLARRGVAVTLYERRPDLRKVDMD
                     AGRSINLALADRGIHALRRAEAYADVAPLLIPMRGRMLHAIDGTLTFAPYGQRAHEVI
                     HSVSRPGLTRALLDHAQRRHGIAPRFLQAARALDFERDELVMLDEVTGDTYTRRLAPL
                     IAADGAGSVVRRAMTGQLGVQVVEDPLDYGYKELTLPAGPGGRHRVEREALHIWPRGG
                     FMLIALPNLDGSFTVTLFLALRGGPDSFAALTTHASVEAFFARHFPDAVTLMPGLARE
                     FFEHPVGRMGTVYADEWSARGRAVLLGDAAHAIVPFHGQGMNACFEDCYELDRLLGQT
                     GDWAQAFGRFTALRKPHTDAIAAMALENFTEMRDTVRDPKFLLRKELSFELERRHPER
                     FIPRYSMVMFHHAIPYAVARERGEIQAAILAALTEHATSLDQIPVERMDAEVSGRLEP
                     LPVEFRAKR"
BASE COUNT         1423 a         3288 c         3333 g         1424 t
ORIGIN      
        1 ggtcgacgga tcggtgaaat gctcgagcat cacctcggcc agttcgatcg cccaatcgag
       61 cgccgtgggc tcccagcgcg cctccagcag ttcgagcagg ccccaggcga gcgaggcgta
      121 gtcgtcgagg tagcccgcgt atttcgaggt gccttccgca tgcaccgcga ggagtcggcc
      181 gtcgcgccag cagcgctcgc gcaggaactg catcgcgcgc acggccgcgt ccgcgtagtc
      241 gtcgcgcgcg agcgcgcggc tcgcgcgggc gagtcccgcg atggcgaggc cgttccagga
      301 ggtcaggatc ttctcgtcac gcccgggcca gacccgcgcg tcacgcacct cgagcagcct
      361 ggcgcgtgcc tcgtccagcc gcgtcgcaac ggcttcttcg ccgagaccga agtcggcggc
      421 cacgtcgccg acgccgcgac gcacgtagag gtgccattgc ccctcgaagt tgggctcctg
      481 gtcgaggccg aagcggcgcg cgaacgcctc gtactgctcg ggctcgagca cgcgctgcac
      541 ttcgtccggc gtccagacgt agaacttccc ctcgtgaccc tcggagtcgg cgtcgagcgt
      601 cgagtagaag gcgccctccg gcgagcgcag gtcgcgcagc agccagtctg ccgtctcgcc
      661 ggtcacgcgg cgaaacaggg gctcgccggt cgcgaccgcg gcctgcgcgt acaccccgag
      721 cagctggccg ttgtcgtaca gcatcttctc gaagtgcgga atcatccaga acgcgtcgac
      781 cgagtagcgg cagaacccgc cgccgagctg gtcgtacagg ccgccttctg ccatgcgcgt
      841 gagcgtgagc gtcgccatgt agagcgcatg caggtccggt gcttcgccgg ccgcggaggc
      901 gcgccagctg cgcagcagca gctcgacgtt ggtcggatgc gggaacttgg gtgcgtcgcc
      961 gaagccgcca aagcgctcgt cgaactcgcg ctgcagcgtg gcgcgcgcga cttcgagcgg
     1021 tccgcggccg agcgtggcgg ccgcgtggtc cggcggcggc tgcaactgcc cgagcacctc
     1081 gacgagcttg tcgccgaacg actcgaggtc ggcccggcgc gtgcggtaga actccgcgac
     1141 tttctccagc accgcacgaa agcccggcat gccgtaacgc tgctcgggag gaaagtacgt
     1201 gcccgagaag aaaggtcgca gcgatcccgg cgcgaggaac acggtgagcg gccagccgcc
     1261 gcccgcctgg ttcatgacct ggtgggccgt ctggtagatg cggtcgatgt ccggccgctc
     1321 ctcgcggtcg accttgacgt tgacgaacag gtcgttcatg agccgcgccg tcgccggatc
     1381 ttcgaacgac tcgtgcgcca tcacgtggca ccagtggcag gccgagtacc cgaccgagag
     1441 caggatcggc ttgtcgagcg agcgcgcaag ctcgagcgcc tcgggccccc acggatacca
     1501 ctcgacggga ttcgccgcgt gctgtcgcag gtaggggctc gtctcgttgg ccagcgcgtt
     1561 gcgaggagcg ggcgaggcag cgggctgcat gggaaaacct gtcgggcgcg gtcggctaga
     1621 atgggcagtc tataacggag ggctgacgcc cggcggcgga aacgcacatg tccacctcga
     1681 tcgcggcgat cgatcattcg gcccccgcac tgccggaact gcgcctcaag ccacgcgaag
     1741 agcggcggct gacggcgggc cacctctgga tcttcagcaa cgaagtcgac accgcgcgca
     1801 cgccgctcac cggtttcgcg cccggcgcga cgtgccgcgt ggtctcgagc cacgaccgct
     1861 ttctcggcta cgcgtacgtc aacccgcacg cgctgatctg cgcgcgcatc gtcgggcgcc
     1921 acccggagca cccgcccggc aggtcgctga tcgtgcaccg gctgcaggtg gcgctggcgc
     1981 tgcgcgagcg gctgtacgac cggccgtact atcgcctggt ctacggtgaa tcggacggat
     2041 tgccggggct cgtgctcgac cgcttcgggg acgtcgtcgt cgggcaggcg ggcaccgccg
     2101 gcatcgaagc gctgaagggc gaggtcgtcg cggccgtgca gaaggtgctc gatccgcgca
     2161 cgttcgtgtg gaagaacgac ggcggcgcgc gcgagctcga aggcctgtcg tcgtacgtcg
     2221 agacggcgtt cggtccgccc gtggacgagg ccatcgtcga ggagaacgac gtgcggttcg
     2281 tcgcgccggt cgggcgcggc cagaagaccg gctggttcta cgaccagtcc gccaaccgcc
     2341 gtgcgctgct caagtacgtg ccggggcggc gcgtgctcga cgtcttctcg tacctcggcg
     2401 cctggggact cgcggcggcg aaggcgggcg cgacggaagt cctgtgcgtg gactcgtccg
     2461 caccggcgct cgagctgctc gagcgctcgg cgcgcgccaa cgcgctcgat cacgtgcgca
     2521 cggagcgtgc cgacgccttc gacgcgctcg cagcgctgcg cgcgggcgca gagaagttcg
     2581 acgtggtcgt gatcgacccg ccggcgttca tcaagcgacg caaggacatc ccgaaggggc
     2641 aggcggcgta tcgcaagctg aaccagctcg cgatgcaggt gctcgcgcgc gacggcatcc
     2701 tcgtgtcgtg ctcgtgctcg catcacctcg ggcaggacga cctgctgcac gcgatccagc
     2761 agtcggcgcg ccacctcgac cgctttgcac aggtcgtcga gatcggcggg cacgcacccg
     2821 accacccgat ccacccggcg attcccgaga cccgctacct caaggcgctg tattgccgcg
     2881 tcgtgcacga ttgagcgcga cgtcgacgag tccgcggcca ggcagcggcg acaacgggta
     2941 agatacgcgg ccgatgttcg tctactcgca cccagatccc gtcgcgctgt cgctcgggcc
     3001 gctgcacgtc cgctggtacg gcctgatgta cctggtcgga ttcctcgcgg cctggtggct
     3061 ggcccgccgg cgtgcgtccc gacccgaatc gtcgtggtcg ccgggcgagg tcgacgacct
     3121 catcttctac tgcgccgtcg gcgtgatcct cggcgggcgc ctgggctgga tgctgttcta
     3181 cggcacggag cggctgatgg ccgacccgct cagcgcaatc cgcatctggg agggcggcat
     3241 gtccttccac ggcggcctgg tcggcgtgat cgccgcgctc gcgctgttcg cgtcgaggcg
     3301 tgggcgccgc atcccggacg tgttcgactt cacggcgccg ctgcccgcga tcgggttcgg
     3361 tgccgggcgc atcggcaact tcatcaatgg cgaactctgg ggcaagccct ccgacgtgcc
     3421 atgggccgtc gtcgtcgacg gcaccccggt gcatgcgtcg cagctctacg aggcggcgct
     3481 cgaaggcctg gcactgttcc tgatcctctg gtggttcacg tcgcggccgc gtttccggtg
     3541 ggcaccgtcg ggcctgttcc tgctctgtta cggtgttttc cggttcggcg tcgagttcgt
     3601 gcgcgtgccg gacgcgaacc gcggctacct gttcctcgac tgggtgacca tgggccaggt
     3661 gctgtcgctg cccatgatcg tggcgggcct cgcgatgctc gcctacgcgt accggcagcg
     3721 cgagccgtcc ggcaactacc gcgcggcctg acggcgatgc ggcagtacca ggacctcctg
     3781 cgacacgtgc tcgagcgggg cgcgcgcaag accgaccgga ccggcaccgg cacgctgtcg
     3841 gtgttcggct ggcagatgcg cttcgacctc gaagaaggct tcccgctggt cacgaccaag
     3901 aagctgcacc tgaagtccat cgtgcacgag ctgctctggt tcctgagcgg cgataccaac
     3961 gtgcgctacc tgcgcgagca cggcgtgacg atctgggacg aatgggccga cgaggaaggc
     4021 gaactcgggc ccgtgtacgg cgcgcaatgg cgcgcgtgga ccgccgcgga cgggcgtcgc
     4081 atcgaccaga tctcgcgagc catcgacctg atccggcgcg acccggactc gcggcgcatc
     4141 ctcgtcaacg cgtggaacgt cggcgaactc gagcggatgg cgctgacgcc gtgccacgcg
     4201 ctgttccagt tctacgtggc ggagggacgc ctgtcctgcc agctctacca gcgcagcgcg
     4261 gacgtgttcc tcggcgtgcc gttcaacatc gcctcgtacg cgctgttgac gcacatggtc
     4321 gcgcagcagt gcgacctgcg gccgggcgaa ttcgtgtgga ccggtggcga cacgcacctg
     4381 tacgtgaacc acgtcgacca ggcgcgcacg cagctcgagc gcacgccttt ccccttgcca
     4441 cggctccgga tccggcgcag gccgccgtcg atcctcgact accagtacga cgacttcgaa
     4501 ttcgtcggct accaggccca ccccgccatc aaggctccca tcgccgtatg accaagcgcc
     4561 cgaagtccgc cccgatcaag cccaggaccg catccaggcc tgcgcccggc gcggccagtc
     4621 cgcaccacgc gcacatgcgc cgcgccgcgg tcacgcgctc gcggcccgcc gacgagaatc
     4681 cgtacgtcgt ggtgcgcaat tcccggattc acggtcgcgg cgtgtacgcg gcgcgccgga
     4741 tccgcaaggg cacgcggatc atcgagtacg tgggcgaccg gatctcgcac gacgaggccg
     4801 acgcgcgcta cgacgcgagg cccgacgacg gccacacgtt cctgttcgtc gtcgacgacg
     4861 agctctgcat cgacgccggc atcggcggca acgccgcgcg gttcatcaac cacaagtgcg
     4921 acgccaactg cgagaccatc atcgaggacc ggcgcgtgtt catcgaggcg ctgcgcacga
     4981 tccagcctgg cgaagaactc ggctacgact atcagctcac ctgggagagc acggacgacc
     5041 ccgaggaact cgcgctctac cgctgcctct gcggcgccgc cgaatgccgg ggcacgatgc
     5101 tcgaccgcgt gccactcgac gagaagcgcc gtcgcgagcg cgcgcgacgc aaggcgcgca
     5161 agcgtgccgc ggtcgacgtc ccgggtgccg ctgcgaagaa gggcaggcgc gccgccgggc
     5221 gcgcccgctg aaccgtggca gcgctcgtct cgatcgtcgt cgcgatggac gagcgcaacg
     5281 ccatcggacg cgcgggcggc ctgccgtggc gcctgcccga cgacttgaag cgcttcaagg
     5341 cgctcacgat cggcaagccg gtcgtcatgg gacgtcgcac ctgggactcg atcggcaagc
     5401 cgcttccagg acgtcacaac gtcgtcgtga cgcggcagcg cggattgaac ctcgacggcg
     5461 cgacgcccgt cgggtcgctc gagcaggcac tggccgtggt cggcgacgtg ccggaggtct
     5521 gcgtcatcgg tggcgccgaa atctaccgcg tcgccctgcc agtggccggc gtgctgcacc
     5581 tgactcgcgt gcacgcgacg ttcgacgccg atgcgttctt tccaccgttc gaaccggcgg
     5641 attgggtcga agtggcgcgc gaagatcgcg ctgctgacga ccggcacggg tgggcgttca
     5701 gcttcctgga gctgcggcgc gcacgccgaa acgcccggac gtcctaagcc gttgtcagag
     5761 ccgtcctacg gacagctacg gtgggggtgt gggactacgc tgctagaatg cgtccggttt
     5821 gcccacggag cgctggatcc gatgacgagg tcgagagtcc gtgtacgacg ttgacgacga
     5881 ccccttcggt ccgttgcgca ggtggttcgg gcactggccg ccgctgctgc tgacggtcgc
     5941 cgcgctcgcg ctcgccacgc tcgtccggtt cgccctcgac gaggcctggt ccgaccgatt
     6001 gccgttcctg ccgtattttc cggcgatcct cctgtgcgcg atggtggcgg ggtggcggta
     6061 cggcgcgctc gccacggtcc tgtccgccct gctgtgcgtg ctgctgaacg tgcgcgtgct
     6121 cgacgtcgcc atgctgaccg cggtcgcttt gttcgtcgcc gccaacgcga tcacgatcat
     6181 gctggcggaa tcggcgcgtc gcgcgcgcgc gcgcgccgag gccgccgccg cggatgcaca
     6241 ggagcgcgag cgccgcttcg aggtgatggc ggacagcgtg ccgctgatca tctgggtgca
     6301 cgatgccgcc ggcggcatac gcttcgtcaa caaggcgtgg gagcagttct tcggcgtcac
     6361 gcaggagcag gcacgtcgcg acggatggca ggcgctggtt cacgacgagg accgcgcagc
     6421 gtacgaaggc cagttcctgg aatgcatccg ggagcgcaaa ccgttccacg ccgaggcgcg
     6481 cgtccgtcgt gccgacggcg cctggcgttt catcgagtcg tacggcgtgc cgcggatcgg
     6541 tgccaacggc gagctcgtcg tattcgcggg cagcaccatg gacgtcaccg agcgccaccg
     6601 ccttgccgtc gagcgcgagc agctgctcga gggcgaacgg gcggcccgct cggaggccga
     6661 gctcgccacg cgcgccaagg acgagttcct ggcgacgctg tcgcacgagc tgcgcacgcc
     6721 gctctccgtc atcgtgctct ggagtcgcat cctggcgcgc aagtacggcg gcacgagcga
     6781 cgaactgcgc aaagggctcg cgctgatcat cgacaacggc atggcgctgt cgcagctgat
     6841 cggcgacctg ctcgacatga gccgcatcgt ctccggacgc gtcacgctcg acatgcggcc
     6901 gatcgacgcg gccgaggtcg tcgcgcaggc ggtcgcctct catcgccccg ccgccgacgc
     6961 ccggcgcatc acgctggccc tcgaggttgg tccgcagccc aagatcgtcc tcggcgatcc
     7021 gacgcgcctg cagcagatcc tgtggaacct gctgtcgaac gcgctcaagt tcacgcacga
     7081 gaacggccac atctgggtca cggcgcggca tcgcggcagg cagctcgaaa tcgccgtgcg
     7141 tgacgacggc gagggcatcg cgcccgagtt cctgccgcag atcttcagcc ggttccgcca
     7201 ggcggacagc acgaccgcgc gacgccacgg tggcctcggc ctggggctgg cgatcgtgaa
     7261 gcagctggtc gagctgcagg gtggcgagat cgaggcgacc agcgacgggc ccgggcgcgg
     7321 gtccaccttc aaggtgacat tgccgctgca cgagtccgcg ttcgcgacgc tcgacacgga
     7381 cagcaccggc agctggcgac gcctcgatcc ggaccgcctg ctcaacccga ggctcgaagg
     7441 cctgcgcgtg ctggcggtcg aagatcaggc ggaaatgctc gaatcgttgc gccagatgct
     7501 ggaggagcag ggcgcgcgcg tgacgacggc cacctccggc gtcgaggcgc tggagacgct
     7561 gcgcacgcac ccggacgatt tcgacgccat ggtcagcgac atcggcatgc cgaccatgga
     7621 tggctacgag ctgatccgcc gcgtccgcaa tgaactcggc ctcggcaccg accggctgct
     7681 cgcggtcgcg ctgaccgcgt acgcccggga cgaggaccgc gcgcgcgcgt tgcagttcgg
     7741 cttccaggcg cacctctcga agccctacca ggtcggccag ctggtcgcga tcgtgaacca
     7801 gctgctggcc gcgcagcgcg tcaagaacca gggcgaagcc gtgctcggcg aagccggcgc
     7861 gacggcgggc tgaccgtcgc ctaccgcttc gcgcggaact ccaccggcag cggctcgaga
     7921 cgtcccgaca cttcggcgtc catccgttcg acggggatct ggtcgaggga ggtcgcgtgc
     7981 tcggtcagcg cggccaggat ggcggcctgg atctcgccgc gctcgcgtgc gacggcgtac
     8041 gggattgcat ggtggaacat caccatcgag tagcgcggga tgaaccgttc agggtggcgg
     8101 cgttcgagtt cgaaactcag ctccttgcgc agcaggaatt tcgggtcgcg gaccgtgtcg
     8161 cgcatctcgg tgaaattctc gagcgccatc gcggcgatcg cgtcggtgtg cggcttgcgc
     8221 agggccgtga agcgcccgaa ggcctgcgcc cagtcgccgg tctggccgag caggcgatcg
     8281 agttcgtagc agtcttcgaa gcacgcgttc atgccctgcc cgtggaaggg cacgatcgcg
     8341 tgcgcagcgt ccccgagcag cacggcacgg ccgcgggccg accactcgtc ggcatacacc
     8401 gtgcccatgc gcccgaccgg gtgctcgaag aactcgcgcg cgaggcccgg catcagtgtc
     8461 acggcgtcgg ggaagtgccg tgcgaagaaa gcttcgacgc tcgcgtgcgt ggtcagcgcc
     8521 gcgaacgagt cggggccgcc gcgcagcgcc aggaacagcg tcaccgtgaa gctgccgtcg
     8581 aggttcggca gcgcgatcag catgaatccg ccgcgcggcc agatgtgcag cgcctcgcgc
     8641 tccacccggt gccggccgcc cgggccggcg ggcagcgtga gctccttgta gccgtagtcg
     8701 agcggatcct cgacgacctg cacaccgagc tgtcccgtca tcgcgcgacg cacgaccgat
     8761 ccggcaccgt cggcggcgat cagcggcgcg agccgccgcg tgtacgtgtc gccggtgacc
     8821 tcgtcgagca tgacgagctc gtcgcgctcg aaatcgagcg cgcgcgcggc ctgcaggaag
     8881 cgcggtgcga tgccgtggcg ccgctgcgcg tggtcgagca gggcacgcgt caggccgggt
     8941 cgcgacaccg aatgtatgac ctcgtgcgct cgctgcccgt acggcgcaaa cgttagcgtg
     9001 ccgtcgatcg cgtgcagcat gcggccccgc atcgggatca gcagtggcgc gacgtcggcg
     9061 taggcctccg cgcgcctcag cgcgtggatg ccgcggtcgg ccagcgccag gttgatggag
     9121 cggccggcgt ccatgtcgac cttgcgcagg tccggacggc gctcgtagag cgtgaccgcc
     9181 accccgcgcc gggccaggaa gatcgccatc agcgcgcccg agaggccggc gccgaggatg
     9241 gtggcgtcgt tcggcgtcaa gctgcgcgct ccagcgtggt cccggcgagg gcgttcaagc
     9301 cgcgagctcg gcgtgcagcg cgtcgacgca gcgccacacg tcgtcgtagc ggttgtagaa
     9361 cggcacgggc gcggcgcgaa tcacgtcggg ctcacgccag tccggcagga tcagccgctg
     9421 gcgcaggccg tcgaacaccg cgcgcgcgcg gtcgcgagaa agtccgac
//
LOCUS       DASPFR010000167         7063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1149175, whole genome shotgun sequence.
ACCESSION   DASPFR010000167 DASPFR010000000
VERSION     DASPFR010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149175"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1287
                     /locus_tag="VD737_04220"
     CDS             91..1287
                     /locus_tag="VD737_04220"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF006719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HXW09796.1"
                     /translation="MFDALETLAPDPILGVTAAFRRDNYPLKVDLGVGVYRDVHGITP
                     VPAAVREAERALIAAQTSKTYVGPAGNLEFNARIVELALGPLAARLGPRIATIQTVGG
                     CGALRLGAELVRTACPDAVIHVSTPTWANHEPLVGSSGLRLERYPYYDPVSRDVDFDA
                     MHDHLARLPRGSVVLLHGCCHNPTGADLDIEQWGALAAVIERRGLLPFVDMAYQGFAD
                     DLERDVAGLRALAEAVPELLLAISCSKNFGLYRERAGALAVIARTPDAAAAVATHQAR
                     LARRMYSMPPDHGAAIAATLLGDASLRSQWAGEVAVMVERMKALRGLLAGRLAERQPN
                     GDFGWLTRQRGMFSLLGLDADQLQALREQHHIYVPADGRMNVAGISELNVDYIADAIA
                     SLTTRR"
     gene            1596..2051
                     /gene="rimP"
                     /locus_tag="VD737_04225"
     CDS             1596..2051
                     /gene="rimP"
                     /locus_tag="VD737_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HXW09797.1"
                     /translation="MLRDTLTALLQPVVEGLGYELWELEYMPGRGNALLRIYIDTGAS
                     EGITVEDCERVSRAASDTLDAEDPIPGNYTLEVSSPGIERPLRTARHFAPYAGEQVFV
                     EMAHVVEERRRFKGRLVAAGAESIEVEVDGRTYRLPIAGIRKAHLAPEL"
     gene            2079..3581
                     /gene="nusA"
                     /locus_tag="VD737_04230"
     CDS             2079..3581
                     /gene="nusA"
                     /locus_tag="VD737_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003235027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HXW09798.1"
                     /translation="MNKEILMVVDAVSNEKGVEKDIIFEALEAALASATRKKYGEEID
                     VRVSINRKTGDYDTFRRWKVFADDSTELEFPDKELRLEDALDISPDAEPGGYVEVPLE
                     SVAFGRIAAQTAKQVIVQKVREAERAQVVEQYKGRVGTLVSGLVKRVDRNGIFVDLGG
                     NAEGFIPREAMIPREPVRTQDRIKAFLKDVRSEPRGPQLFLSRTAPEFLIELFKLEVP
                     EVGQGLINILGAARDSGARAKIAVRSNDPRIDPVGACVGMRGSRVQAVSNEIAGERVD
                     IIPYDDNPAQFVINAMQPAEVSSIVVDEEAHSMDIAVAEEKLSQAIGRGGQNIRLASE
                     LTGWKLNIMSEQAAEEKSESEQKALRELFMQQLDVDEDVAGILVQEGFSSIEEIAYVP
                     QAELAGIEEFDEEIVQELRNRARDVLLTQAIASEETIEKAAPAADLLEVDGMDDALAA
                     ELARHGIVTREDLADQAVDDLLDIPSIDEKRAGELILAARKHWFEQGERA"
     gene            3598..6183
                     /gene="infB"
                     /locus_tag="VD737_04235"
     CDS             3598..6183
                     /gene="infB"
                     /locus_tag="VD737_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HXW09799.1"
                     /translation="MAEVTVTQFAEVLKVPVEKLLVQLGEAGIQVGGADDIISDEAKM
                     ELLTHLRRAHGRQEDAAAPRKITLQRKSQQEIRLASTQGRARTVAVEVRRKKTYLNRS
                     VIEEEQRQRQEDADRQRNAEENAETARHDQERAEVERAAAEIRERERIEAENLRVEEE
                     QARQRAEADARVAAEEAARERTERERAEAERKALETVQPPTRPAGIRVARPAEPMPQP
                     APGSARPAPGGSGKYGRRELHVSADARASFKKKQQHPAGRFRRSGQVQVETRHGFVEP
                     TAPVKREVQIPETITVGELASRMAIKANEVIKTMMKMGVMATINQPIDQDTATLVVEE
                     FGHTAVALKEDQLEDQLLATASDVELLPRPPVVTIMGHVDHGKTSLLDYIRRTKVAAG
                     EAGGITQHIGAYQVETPRGPITFIDTPGHAAFTAMRARGANVTDIVILVVAADDGVMP
                     QTVEAIQHARAAEVPIVVAVNKIDKHDADPDRVRNDLGKQNVIPEEWGGDVMFVNVSA
                     RTGDGVDRLLESVLLQAEVLDLKSPQAGHAVGVVLESSIEKGRGPVATILVKRGTLRT
                     GDPIIAGQEFGRVRALFDALGKQVPEAGPAVPVQVLGLSDAPNAGDDMLVVESERKAR
                     EVALYRQGKYRDVRLAGATKKVEDVFSQMGEAAGQTVNVIVKADVQGSAEALRDALSK
                     IGTDEVTVKVIASGVGGITESDVSLAAASRARVIGFNVRADGAARTAMKDQGVEVNYY
                     SIIYEALDDMRALVSGMLKPEIKEQIVGLAEVREVFRSSKFGTVAGCLVTEGFIKRGN
                     PVRVLRDNVVIHQGELDSLRRFKDDVGEVRAGTECGIGVVKYNDIRVGDQIECFARVE
                     VARTA"
     gene            6194..6670
                     /gene="rbfA"
                     /locus_tag="VD737_04240"
     CDS             6194..6670
                     /gene="rbfA"
                     /locus_tag="VD737_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HXW09800.1"
                     /translation="MPREVPRARRIAEQVQRTLSQLLLREVRDPRLKPMTVTHVKVSA
                     DLTHVWVKYTLLSGDSHDALQREILDEAATYLRGPLGRALRLRVAPTLHFAPDEELER
                     GNRLDELITRAVREDRAKSPGSTDDDAAADEAADPADDDDARYDSDDDDAAPDDRP"
     gene            6670..>7063
                     /locus_tag="VD737_04245"
     CDS             6670..>7063
                     /locus_tag="VD737_04245"
                     /inference="COORDINATES: protein motif:HMM:NF013660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09801.1"
                     /translation="MRATPTPPAPARKRWRAVDGIVLLDKPPGLTSTQALQRVRRALQ
                     AEKAGHAGTLDPMATGMLPLCFGQATKACGTLLGQRKAYRATVRLGSATDTGDADGHV
                     VAQRDVSPATTGQVEDALRGLRGERTQVP"
BASE COUNT         1199 a         2388 c         2548 g          928 t
ORIGIN      
        1 gaccagaagg tcgtgcaggc gcgcgccggc gcgacctgaa caccagcgcg tttcggtggc
       61 atgatgcgcc gatccagcac aggcgatgcc atgttcgacg cgctcgaaac gcttgctccc
      121 gacccgatcc tgggcgtgac cgcggccttt cgccgcgaca actatccgct caaggtggac
      181 ctcggagtcg gcgtctaccg tgacgtgcac ggcatcaccc cggtgccggc cgccgtccgg
      241 gaggccgagc gtgcgctgat cgcggcgcag acgagcaaga cgtacgtcgg accggctggc
      301 aacctcgagt tcaatgcacg catcgtcgaa ctcgcgctcg gtccgctcgc ggcgcgtctc
      361 ggtccgcgca tcgcgaccat ccagaccgtc ggcggctgcg gggcactgcg actcggcgcc
      421 gaactcgtgc gcaccgcctg ccctgacgcc gtgatccacg tcagcacgcc cacctgggcc
      481 aaccacgagc cgctggtcgg cagttccggc ctgcgcctcg agcggtaccc gtactacgac
      541 ccggtctccc gagacgtcga cttcgacgcc atgcacgacc atctcgcgcg cctgccgcgg
      601 ggttccgtcg tcctgctgca cggctgctgc cacaacccga ccggcgccga cctcgacatc
      661 gagcagtggg gcgcgctggc ggccgtgatc gagcggcgtg ggctgctgcc gttcgtcgac
      721 atggcatatc agggtttcgc cgacgacctc gagcgtgacg tcgcgggctt gcgtgcgctc
      781 gccgaggccg tgccggaact gctgctcgcg atctcgtgct cgaagaactt cgggctctat
      841 cgcgagcggg ccggcgccct cgccgtgatc gctcgcacgc cggacgcggc ggctgcggtc
      901 gccacgcacc aggcgcgcct ggcccggcgc atgtactcga tgcctcccga ccacggcgcc
      961 gccatcgcgg ccacgctgct gggcgatgcg agcctgcgtt cgcaatgggc aggggaagtg
     1021 gccgtcatgg tggagcgcat gaaggcgctg cgcgggctgc tggcggggcg gctcgccgag
     1081 cggcagccca acggcgactt cggctggctc acccgacagc gcggcatgtt ctcgctgctc
     1141 ggcctcgacg cggaccagct gcaggccctc cgtgagcagc accacatcta cgtgccggcc
     1201 gacggccgca tgaacgtggc cgggatcagt gagctcaacg tcgattacat cgccgacgcg
     1261 atcgccagcc tgacgacgcg gcgctgacgg cagcgttaca gcgcggcggg cagccctcgc
     1321 ggccattggt catcggcccg ccgccctgac tagcccagcc ctcggtcttt gctatagaat
     1381 ccgcggtccg aggcgttacg taagtcgcag gcgcgaccgc ggagcgcgtt cggccggcag
     1441 gcacctcgcg gcgcccgtcg gagcggcggg agattcgcca gagaggtccc ccgagcgggg
     1501 atttttcttt ttgacggcga ccatgccggt cgccggaaga atgggccgcg ggcccatttt
     1561 ttgtttgtgg acgcggcagg agcgggcgag accagatgct cagggacacg ctgacggcgt
     1621 tgctgcagcc ggtggtcgag gggctcgggt acgagctctg ggaactcgaa tacatgccgg
     1681 gtcgcggcaa cgcgctgctg cgcatctaca tcgacacggg ggccagcgag ggcatcacgg
     1741 tcgaggactg cgagcgggtg agccgggcgg cgagcgacac gctcgatgcc gaggatccga
     1801 tcccgggcaa ctacacgctc gaggtgtcct cgccgggcat cgagcggccg ctcaggacgg
     1861 cacgacattt cgcgccgtac gccggcgaac aggtgttcgt ggagatggcg cacgtggtcg
     1921 aggagcggcg ccggttcaag ggccggctgg tcgcggcagg tgcggagtcg atcgaggtgg
     1981 aagtggacgg ccggacgtac cggctgccga ttgcaggcat acgcaaggcg cacctggcgc
     2041 cggagctttg acgggcgctg ggaagaggac gacggacgat gaacaaagag atcctgatgg
     2101 tcgtggacgc cgtttcgaac gaaaagggcg tcgagaagga catcatcttc gaagcgctcg
     2161 aggccgcgct cgcctccgcc acgcgcaaga aatacggcga ggagatcgac gtgcgcgtgt
     2221 cgatcaaccg caagaccggc gactacgaca cgttccggcg ctggaaggta tttgccgacg
     2281 attccaccga gctcgagttc ccggacaagg agctgcgcct cgaggacgcg ctcgacattt
     2341 cgccggacgc cgagccgggt ggctacgtcg aggtgccgct cgagtcggtg gccttcggcc
     2401 gcatcgccgc gcagaccgcc aagcaggtca tcgtgcagaa ggtccgcgag gccgagcgcg
     2461 cgcaggtggt cgaacagtac aagggccgtg tcggcacgct ggtgtcgggc ctggtcaagc
     2521 gcgttgaccg caacggcatc ttcgtcgacc tgggcggcaa cgccgaaggc ttcatcccgc
     2581 gcgaggcgat gatcccgcgc gagccggtgc gcacgcagga ccggatcaag gccttcctga
     2641 aggacgtacg ctccgagccg cgtggcccgc agctgttcct gtcgcgcacg gcgcccgagt
     2701 tcctgatcga actgttcaag ctcgaggtgc ccgaggtcgg ccagggcctg atcaacatcc
     2761 tcggcgccgc gcgcgattcc ggtgcgcgcg ccaagatcgc ggtgcgctcg aacgacccgc
     2821 gcatcgatcc cgtgggcgcc tgcgtcggca tgcgcggctc gcgcgtgcag gcggtctcga
     2881 acgagatcgc cggcgagcgc gtcgacatca tcccgtacga cgacaacccg gcgcagttcg
     2941 tgatcaatgc gatgcagccg gccgaagtga gctcgatcgt cgtcgacgag gaagcgcact
     3001 cgatggacat cgccgtggcc gaggagaagc tctcgcaggc gatcggccgc ggcggccaga
     3061 acatccggct cgcgtccgag ctgaccggct ggaagctcaa catcatgagc gagcaggcgg
     3121 cggaggagaa gagcgagtcg gagcagaagg cgctgcgcga gctcttcatg cagcagctcg
     3181 acgtcgacga ggacgtcgcc ggcatcctcg tgcaggaagg tttctcgagc atcgaggaga
     3241 tcgcctacgt gccgcaggcc gagctcgcgg gcatcgagga gttcgacgag gagatcgtgc
     3301 aggaactgcg caaccgcgcg cgcgacgtgc tgctcacgca ggcgatcgcg agcgaggaga
     3361 ccatcgagaa ggccgccccg gcggccgacc tgctcgaggt ggacggcatg gacgatgcgc
     3421 tggccgcgga actcgcgcgc cacggcatcg tgacccgcga ggatctcgcg gaccaggcgg
     3481 tcgacgacct gctcgacatc ccgagcatcg acgagaagcg cgcgggcgag ctgatcctcg
     3541 ccgcgcgcaa gcactggttc gaacagggcg agcgggcgtg agcggaggtc tgattacatg
     3601 gcggaagtga cggtcacgca gtttgccgag gtcctgaagg ttccggtcga gaagctgctg
     3661 gtgcagctcg gcgaggccgg catccaggtc ggcggtgccg acgacatcat cagcgacgag
     3721 gccaagatgg aactcctgac gcacctgcgc cgcgcgcacg gtcgccagga ggatgccgcg
     3781 gccccgcgca agatcacgct gcagcgcaag tcgcagcagg agatcaggct cgcctcgacg
     3841 cagggccgcg cgcgcaccgt cgcggtcgag gtgcgtcgca agaagaccta cctcaaccgc
     3901 tcggtcatcg aggaagagca gcgccagcgg caggaagatg ccgaccggca gcgcaacgcc
     3961 gaggagaacg cggagaccgc gcgtcacgac caggaacgtg ccgaggtcga gcgtgccgcg
     4021 gccgagatcc gcgagcgcga gcgcatcgag gccgagaacc tccgcgtcga ggaagagcag
     4081 gcgcgccagc gggcggaggc cgacgcgcgc gtcgccgccg aagaagcggc gcgcgaacgc
     4141 accgagcggg agcgggccga ggccgagcgc aaggcgctcg agaccgtgca gccgccgacg
     4201 cgcccggccg gcatccgcgt ggcccgtccg gccgagccga tgccacagcc ggcgcccgga
     4261 tcggcgcgcc ccgcaccggg cgggtcgggc aagtacggcc gccgcgaact ccacgtttcc
     4321 gcggacgcgc gcgcgagctt caagaagaag cagcagcacc ccgccggacg tttccgccgc
     4381 tccggtcagg tgcaggtcga gacgcgtcac ggattcgtgg agcccaccgc gcccgtcaag
     4441 cgtgaggtgc agatcccgga gacgatcacg gtcggcgagc tcgcgagccg catggcgatc
     4501 aaggccaacg aagtcatcaa gacgatgatg aagatgggcg tgatggccac gatcaaccag
     4561 ccgatcgacc aggacaccgc gacgctggtg gtcgaggagt tcggccacac ggcggtcgcg
     4621 ctcaaggagg accagctcga ggaccagctg ctcgcgacgg cctccgacgt cgaactgctg
     4681 ccacggcccc ccgtcgtgac catcatgggt cacgtcgacc acggcaagac ctcgctgctc
     4741 gactacatcc gccgcaccaa ggtggcggcg ggcgaggccg gcggcatcac gcagcacatc
     4801 ggcgcctacc aggtcgagac gccgcggggt cccatcacgt tcatcgacac tccgggccac
     4861 gcggcgttca cggcgatgcg agcgcgcggg gccaacgtca ccgacatcgt gatcctggtg
     4921 gtcgcggcgg acgacggcgt catgccgcag acggtcgagg cgatccagca cgcgcgcgcg
     4981 gccgaggtgc cgatcgtggt cgccgtcaac aagatcgaca agcacgacgc cgacccggac
     5041 cgcgtgcgca acgacctcgg caagcagaac gtgatcccgg aggagtgggg cggcgacgtc
     5101 atgttcgtca acgtctccgc gcgcaccggc gacggcgtcg accggctgct cgagtcggtg
     5161 ctgctgcagg cggaagtgct cgacctcaag tcgccgcagg cgggtcacgc cgtgggcgtc
     5221 gtgctcgagt cgagcatcga gaaaggccgc gggccggtgg cgaccatact ggtcaagcga
     5281 ggcacgctca ggaccggcga cccgatcatc gcgggccagg aattcggccg cgtccgtgcg
     5341 ctgttcgacg cgctcggcaa gcaggtgccc gaggcgggcc ctgcggtgcc ggtgcaggtc
     5401 ctggggctct cggacgcgcc gaacgcgggc gacgacatgc tggtcgtcga gagcgagcgc
     5461 aaggcgcgcg aagtcgcgct gtaccgccag ggcaagtatc gcgacgtgcg tctggcgggc
     5521 gcgacgaaga aggtcgagga cgtcttctcg cagatgggcg aagcggccgg gcagaccgtc
     5581 aacgtcatcg tcaaggcgga cgtgcagggc agcgccgagg cattgcgcga tgcactgtcc
     5641 aagatcggca ccgacgaggt cacggtcaag gtcatcgcga gcggcgtggg cggcatcacc
     5701 gagtccgacg tctcgctggc ggcggcgtcg cgtgcgcggg tcatcggctt caacgtccgc
     5761 gccgacggcg cggcgcgcac ggccatgaag gaccagggcg tcgaggtcaa ctactactcg
     5821 atcatctacg aggcgctcga cgacatgcgc gccctggtct ccggcatgtt gaagcccgag
     5881 atcaaggagc agatcgtggg cctggccgag gtgcgcgagg tgttccgctc gagcaagttc
     5941 ggcacggtgg cgggctgcct cgtgacggag ggcttcatca agcgcggcaa cccggtgcgc
     6001 gtcctgcgcg acaacgtcgt catccaccag ggcgagctcg actcgctgcg ccgcttcaag
     6061 gacgacgtgg gcgaggtccg ggccggcacc gagtgcggca tcggcgtggt caagtacaac
     6121 gacatccgcg tgggcgacca gatcgagtgc ttcgcgcgcg tcgaagtggc gcgcacggcc
     6181 tgacggggac gccatgccac gcgaggtacc gcgcgcgcga cggatcgccg aacaggtgca
     6241 gcgcaccttg tcgcagctgc tgctgcgcga ggtgcgtgac ccgcgcctca agccgatgac
     6301 cgtcacgcac gtgaaggtct cggccgacct cacgcacgtc tgggtcaagt acacgctgct
     6361 gtcgggcgac tcgcacgacg cgctgcagcg cgaaatcctc gacgaagcgg ccacgtacct
     6421 gcgcgggccg ctcgggcgcg cgctgcggtt gcgcgtcgcg ccgacgctgc atttcgcacc
     6481 ggacgaagaa ctggagcgcg gcaaccggct cgacgagctg atcacgcgcg cggtgcgcga
     6541 ggaccgcgca aagagccccg ggtccaccga cgacgacgcg gccgccgacg aagccgccga
     6601 cccggctgac gacgacgacg cgcgctacga ttccgacgac gacgacgcgg cccctgacga
     6661 tcgcccgtga tgcgagcgac acccacgccc cccgcgcccg ctcgcaagcg ctggcgcgcg
     6721 gtggacggca tcgtgctgct cgacaagccg ccgggcctga cctccacgca ggcgctgcag
     6781 cgcgtgcgac gcgcgctgca ggccgagaag gccggccacg ccggcacgct cgatccgatg
     6841 gccacgggca tgctgccgct gtgcttcggc caggcgacga aggcctgcgg cacgttgctc
     6901 gggcagcgca aggcctatcg cgccacggtc cgcctcggta gcgccaccga cacgggcgat
     6961 gccgacgggc acgtcgtggc acagcgcgac gtgtcgccgg cgacgacggg gcaggtcgag
     7021 gacgccttgc ggggcctgcg aggcgagcgc acgcaggtgc ccc
//
LOCUS       DASPFR010000168         6813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_783441, whole genome shotgun sequence.
ACCESSION   DASPFR010000168 DASPFR010000000
VERSION     DASPFR010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6813
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783441"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            374..577
                     /locus_tag="VD737_04250"
     CDS             374..577
                     /locus_tag="VD737_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09802.1"
                     /translation="MLKFLLWVLLLVVCWPLALLVLVLWPLVWLLTLPFRLVGIAVEG
                     VFELLRAVLLLPARLLGGRPGRA"
     gene            complement(596..2608)
                     /locus_tag="VD737_04255"
     CDS             complement(596..2608)
                     /locus_tag="VD737_04255"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HXW09803.1"
                     /translation="MTSRLPLVVFMPSGKRGHFPIGTPLLTAARQLGVDIDSVCGGRA
                     ICGRCQVLVAEGEFAKHGVRSHAASLSPRSGTEQRYETRRGLAEGRRLSCQARIEGDL
                     VVDVPPESQVHRQVVRKAAEARDIELYPLVKLHYVEVAEPDMHDPTGDLQRLEAALER
                     EWQLRDLDCDLSVLQRLQPVLRQGQWKVTAAVHDASRIVAVWPGFHETVHGLAVDVGS
                     TTIAAHLCNLVTGEVVASAGRMNPQIRYGEDLMSRVSYSMMNPGGAAQMTQVVREALS
                     DIARELAATAGVECDSILEVTLVGNPIMHHLVLGIDPVELGGAPFALAIDRAVTLTAA
                     ELGIALHPSARVYALPCIAGHVGADAAGVILAERPDLADEMTLLVDVGTNAEIVLGDR
                     RRLVACSSPTGPAFEGAQISCGQRAAPGAIERVRIDRATLEPRFQVIGSELWSDEPGF
                     ADAVGSPGVTGVCGSGIIEVIAGMYLAGIITQDGSFDGSLASRSPRVVPDGRTFSYVL
                     HEDAHRTLAITQNDVRAIQLAKAALMAGIRLLMERLGVTTVDRIRLAGAFGAHIDVKY
                     AMVLGMIPDCDLSKVTSAGNAAGTGARIALLDSRSRATIEALVRQVEKIETAIEPRFQ
                     KHFVEAMAIPHKTAECPNLRTVVDLPPPKFSGSADGRRRTSRRPVR"
     gene            complement(2610..2948)
                     /locus_tag="VD737_04260"
     CDS             complement(2610..2948)
                     /locus_tag="VD737_04260"
                     /inference="COORDINATES: protein motif:HMM:NF025142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HXW09804.1"
                     /translation="MASLIVISWRDVPAQVVVKRGRDVAKVQLSARFMEAVDRAAMRA
                     GRGSSDAYLEDWRRSPPRPCGDDLHAEAAAEAARIEARYSDDDLEALIRAQGIESPPP
                     EKTPRRDEAG"
     gene            complement(2936..3586)
                     /locus_tag="VD737_04265"
     CDS             complement(2936..3586)
                     /locus_tag="VD737_04265"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HXW09805.1"
                     /translation="MSSETRSLLIACGALAREIVALKRLNGWRELDVQCLPPELHNRP
                     ERIPGAVRAAIAAARGRYRQIFVAYADCGTGGLLDRVLHDERVERLPGAHCYEFFATG
                     PRFAALADEEPGTFYLTDFLVRHFERLVVTGLGIDRHPELAQEYFRNYRRMLYLVQAP
                     DAGLSAAARSFAQRFGLQYVEHATGYGQLETSLRSWAGLASSRDTGVTEQGVTWHR"
     gene            complement(3600..4307)
                     /locus_tag="VD737_04270"
     CDS             complement(3600..4307)
                     /locus_tag="VD737_04270"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HXW09806.1"
                     /translation="MTDETTTAEPFDLDSLDDGELVEQVHDDLYNGLRDEVVEATQIF
                     LRRGWAADKVLNETLVEGMRIVGVDFRDGILFVPEVLLAANAMKAGMEVLRPLLAETG
                     AEPIGKVVIGTVKGDIHDIGKNLVSMMLEGAGFEVFNIGINNSVEKYLAALEEHQPDI
                     LGMSALLTTTMPYMKVVIDTLKEQGLRDRYIVLVGGAPLNDEFAQAIGADAYCRDAAV
                     AVETAKSLIAARRAARR"
     gene            4410..5297
                     /gene="folD"
                     /locus_tag="VD737_04275"
     CDS             4410..5297
                     /gene="folD"
                     /locus_tag="VD737_04275"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: protein motif:HMM:NF010783.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HXW09807.1"
                     /translation="MTAQVIDGRKIAEQTRAEHAAGASRLTAATGVVPHLAAVLVGED
                     PASAVYVRNKQLACQKAGMKSSLHRLPASTSERGLLDLVAQLNADPDVHGILVQLPLP
                     KGIDTASVLDAIDPSKDVDGFHPENVGLLQQGRPRFQPCTPAGVMRLLQVSGIDTRGR
                     HAVVIGRSDIVGKPMATLLAQKGSDATVTLCHSRTADIAHHTRQADLVVAAVGIPRFV
                     QGDWLRRGAVVIDVGINRVDDKLVGDVDYAQAATVASAITPVPGGVGPMTIAMLLGNT
                     LLAAEQLCRSRERSARVGG"
     gene            complement(5306..6118)
                     /gene="xth"
                     /locus_tag="VD737_04280"
     CDS             complement(5306..6118)
                     /gene="xth"
                     /locus_tag="VD737_04280"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HXW09808.1"
                     /translation="MSTTTTRIATYNVNGIRARLPHLLRWLEREQPDVACLQELKAVS
                     EGFPILDIRAAGYEAIWHGQRSWNGVAILTRGIKPVEVRRALPGDPDDEHSRYVEAEV
                     GEFIIGCLYLPNGNPQPGPKFDYKLRWFERLIEHAATLYGVGRPVVLCGDYNVVPTDF
                     DIYNPRSWLKDALLQPESRECYQRLLAQGWTDALRKLYPEERIYTFWDYFRQSWQRNA
                     GLRIDHLLLNAEASARLREGGVDRWVRDQEKPSDHAPTWVRLAGPLSANSGR"
     gene            complement(6128..>6813)
                     /locus_tag="VD737_04285"
     CDS             complement(6128..>6813)
                     /locus_tag="VD737_04285"
                     /inference="COORDINATES: protein motif:HMM:NF027666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase/phosphatase domain-containing protein"
                     /protein_id="HXW09809.1"
                     /translation="TLDVGCLKRQYRDRLLSLRAVDDMVGSLVTTLQRLDEWSRTVFI
                     FTSDNGWFYGQHRLTDKILGYEESIRVPLYIRAPGFPGQATTRAALNNDLASTVADFA
                     GIEPGLRVDGRSLLPLMRNPAETMWRKRFLVEFLGNVETDRVPPRAPFSAVRTTNLSR
                     ATPPDQIYVSWKDGLGSEEFYDLRTDPLQLSSQHDNAAWASVRNMLAGWLADFRACAR
                     GSCQTLENQ"
BASE COUNT         1010 a         2293 c         2394 g         1116 t
ORIGIN      
        1 gcaggaactt catcatccgt cggccctccg tcggtttcag catcgcacgc gcccacgacc
       61 ttgcgcgaca gttcatcgcc ctgctcgcac gtttcggccc gcttgaacgg cacgcaaccg
      121 tgacgccgag gagccatccg ggccagaacc gattcgccgg gctgtgcgag gcatggatgc
      181 cgagcccaga gcccccaagg atgggtttac ggcgtcccgg cgaatcgtgt tgtggaccgg
      241 gtggccccgc ggcactccag cctgcaagca ccgcgtctag cattcgcggt gccgagtcgt
      301 gcgggtagag cgacaagccg tcgcgcaaag ctgcctgcgc atgcgattct ggcgtcgtgg
      361 aggacaagga ccgatgttga agttcctgct ctgggtgctg ctgctcgtcg tgtgctggcc
      421 gctggcgctg ctcgtgctgg tgctgtggcc gctcgtctgg ctgctgacgt tgccgttcag
      481 gctcgtcggg atcgcggtcg aaggcgtgtt cgagctgttg cgcgcggtgc tgctgttgcc
      541 ggcgcggctg ctgggcgggc ggccgggccg cgcctgacgg cgccgggcgc cgacctcagc
      601 gcacgggccg gcgactcgtg cgacggcggc cgtcggcgga accgctgaac ttcggcggcg
      661 gcaggtccac gaccgtgcgc aggttcgggc actccgccgt cttgtgcggg atcgccatcg
      721 cctcgacgaa gtgcttctgg aatcgcggct cgatcgccgt ctcgatcttc tcgacctgtc
      781 ggacgagcgc ctcgatcgtc gcgcgcgagc gcgagtcgag cagggcgatg cgggcgccgg
      841 tgccggccgc gttgccggcg gacgtcacct tcgacaggtc gcagtccggg atcatgccca
      901 gcaccatcgc gtacttgacg tcgatgtgcg caccgaaggc gccggcgagc cgtatccggt
      961 cgaccgttgt cacgccgagc cgctccatca gcaggcggat gcccgccatc agcgcggcct
     1021 tggcgagctg tatcgcgcgc acgtcgttct gcgtgatcgc gagggtgcgg tgcgcgtcct
     1081 cgtgcagcac gtatgagaac gtccgtccgt caggcaccac gcgtggcgag cgtgaggcga
     1141 gcgagccgtc gaacgacccg tcctgagtga tgatcccagc gaggtacatc ccggcgatca
     1201 cctcgatgat tcccgagccg cagacgccgg tgacgccggg gctgccgacc gcgtccgcga
     1261 atccgggctc gtccgaccac agctcggagc cgatgacctg gaaccgcggc tcgagcgtcg
     1321 cacggtcgat gcgcacgcgc tcgatggcgc caggggcggc acgctggccg cagctgatct
     1381 gcgcaccttc gaacgcgggt cccgtggggc tcgagcaggc gacgagcctg cgtcggtccc
     1441 cgagcacgat ctccgcgttg gtgcccacgt ccacgagcag cgtcatttca tcggcgaggt
     1501 cgggccgctc cgccagtatc acgccggcgg catcggcgcc cacgtggccc gcgatgcagg
     1561 gcagggcgta gacgcgtgca ctggggtgca gcgcgatccc gagctctgcc gccgtcagcg
     1621 tcaccgcccg gtcgatcgcg agcgcaaacg gcgcgccgcc gagttccacc ggatcgatgc
     1681 cgagcaccag gtgatgcatg atcggattgc cgaccagcgt gacctccagg atcgagtcgc
     1741 attccacgcc ggccgtggcc gccagttcgc gggcgatgtc gctcagtgct tcgcggacga
     1801 cctgggtcat ctgcgccgcg ccgcccgggt tcatcatcga atacgagacg cggctcatca
     1861 ggtcttcgcc gtagcggatc tgcgggttca tgcgccccgc agaggcgacg acctcgccgg
     1921 tgacgaggtt gcagaggtgt gcggcgatgg tggtcgagcc cacgtcgacc gcgagcccgt
     1981 gaaccgtttc gtggaaccct ggccacaccg cgacgatccg cgaggcgtcg tgcacggccg
     2041 ccgtgacttt ccactggcct tgccgcagca ccggctgcag tcgctgcagg accgacaggt
     2101 cgcagtcgag gtctcgcagc tgccactcgc gttcgagtgc tgcctcgagt cgctgcaggt
     2161 cgccggtcgg gtcgtgcatg tccggttcgg cgacctcgac gtagtgaagc ttcaccagcg
     2221 gatacagctc gatgtcgcgg gcttccgcag ccttgcgcac cacctggcgg tgcacctggc
     2281 tctctggcgg cacgtcgacc acgaggtcgc cctcgatgcg ggcctggcac gagagtcgtc
     2341 gtccttcggc caagcccctg cgcgtctcgt agcgctgttc ggtcccggag cgcggggaga
     2401 gcgacgccgc gtgcgaccgc accccgtgct tcgcgaactc gccttcggcc accagcacct
     2461 ggcaccgccc gcagatcgcg cggccaccgc agacggagtc gatgtcgacg ccgagctggc
     2521 gagctgcggt cagcaacggc gtcccgatcg gaaaatgccc gcgcttgccg gacggcatga
     2581 acacgacgag cggcaggcgc gacgtcacct caaccggcct cgtcgcggcg cggcgtcttc
     2641 tccggcggcg gcgactcgat gccctgcgcg cggatcagcg cctcgaggtc gtcgtcgctg
     2701 tagcgggctt cgatgcgggc ggcctcggcg gccgcctcgg cgtggaggtc gtcgccacag
     2761 ggccgcggtg gactgcggcg ccagtcctcg agatacgcgt cggagctgcc gcgtccggcg
     2821 cgcatcgcag cacgatcgac ggcctccatg aaccgcgccg acagctggac cttggcgacg
     2881 tcacgacctc gcttgacgac gacctgcgcg ggcacgtcgc gccaggaaat gacgatcaac
     2941 gatgccacgt cacgccttgc tcggtgacgc cggtgtcgcg cgagctggcg aggcccgccc
     3001 agctgcgcag gctcgtctcg agttgcccat agccggtcgc gtgctcgacg tactggaggc
     3061 cgaagcgctg cgcgaaactg cgagccgcgg ccgagagccc ggcgtcaggc gcctggacca
     3121 ggtacagcat gcgtcggtag ttccggaaat actcctgggc gagctcgggg tggcgatcga
     3181 tgccaagccc ggtcacgacc agccgctcga agtgtcgcac caggaagtcc gtcaggtaga
     3241 acgtgcccgg ctcctcgtcc gccagcgcgg caaatcgggg cccggtcgcg aagaactcgt
     3301 agcagtgggc gcccggcaat cgttcgacgc gttcgtcgtg cagcacgcgg tccagcaatc
     3361 cgcccgtgcc acagtcggcg taagcgacga agatctgccg gtatcgaccg cgcgccgcgg
     3421 cgatggcggc gcgcaccgcc ccgggtatgc gttcgggacg attgtgcagt tcaggcggca
     3481 ggcactgcac gtcgagttcg cgccagccgt tgagccgctt gagcgcgacg atctcgcgag
     3541 cgagggcgcc gcaggcgatc aagagcgacc gggtctcgct gctcacggcc cgcggccctt
     3601 catcgccgcg ctgcgcgacg cgcggcgatg aggctcttgg ccgtctcgac ggcgacggcg
     3661 gcgtcgcggc agtacgcatc ggcgccgatg gcctgcgcga actcgtcgtt gagcggcgcg
     3721 ccgcccacca gcacgatgta ccggtcgcgc aggccctgtt ccttcagcgt gtcgatgacc
     3781 actttcatgt acggcatcgt cgtggtgagc agcgccgaca tgccgaggat gtccggctgg
     3841 tgctcctcga gcgccgcaag gtacttctcg accgagttgt tgatgccgat gttgaagacc
     3901 tcgaagcccg cgccctcgag catcatcgac acgaggttct tgccgatgtc gtggatgtcg
     3961 cccttgacgg tgccgatgac gaccttgccg atgggttcgg cgcccgtctc ggccagcagg
     4021 gggcgcagca cttccatgcc ggccttcatc gcgttggccg cgagcagcac ttcgggcacg
     4081 aacaggatgc cgtcgcggaa gtcgacgccg acgatccgca ttccctcgac cagcgtctcg
     4141 ttgaggacct tgtcggcggc ccagccgcgg cgcaggaaga tctgcgtcgc ctcgacgact
     4201 tcgtccctga ggccgttgta gaggtcgtcg tgcacctgct cgaccagctc cccgtcgtcg
     4261 aggctgtcga ggtcgaaagg ttccgccgtg gtcgtctcgt ccgtcatcgg ctgcgaactc
     4321 tatcaccggt cggctcgcga agggcacggg cgggaacgct atgatcagtg tccggagggc
     4381 ccgtcgtacc gggccggagc gaatactcga tgacggcaca ggtgatcgac ggcaggaaga
     4441 tcgcagagca gacgcgagcc gagcacgcgg cgggtgcgag ccggttgacg gcggccacgg
     4501 gggtcgttcc gcacctggcc gcggtgctgg tcggtgagga cccggcgagc gcggtgtacg
     4561 tgcgcaacaa gcagctcgct tgccagaagg ccggcatgaa gagctcgctg cacaggctgc
     4621 cggcgtcgac gtcggagcgt gggttgctcg acctcgtggc gcagctcaat gccgatccag
     4681 acgtgcacgg catcctggta cagctgccgc ttccgaaggg catcgacacc gcgtccgtgc
     4741 tcgatgcgat cgatccgtcg aaggatgtcg atggctttca ccccgagaac gtcgggctgc
     4801 tgcagcaggg caggccgcgc tttcagccct gcacgccggc aggggtcatg cggttgctgc
     4861 aggtgtcggg catcgatacg cgcggccgtc acgcggtcgt gatcggccgc agcgacatcg
     4921 tcggcaagcc gatggcgact ctgctcgcgc agaaaggctc ggacgcgacc gtgacgctct
     4981 gccacagtcg caccgccgac atcgcgcatc acacgcgcca ggccgacctc gtcgtcgccg
     5041 ccgtgggcat acctcggttc gtgcagggcg actggctccg tcgcggcgcg gtcgtgatcg
     5101 acgtggggat caatcgcgtc gacgacaagc tcgtgggcga cgtcgactat gcgcaggccg
     5161 cgacggtggc ctccgcgatc acgccggtgc cgggcggcgt cgggccgatg acgatcgcca
     5221 tgctgctcgg caacacgctg ctcgccgccg agcaactctg ccgttcgcgg gagagatcgg
     5281 cccgcgtggg cgggtgaggg aggtcttaac gcccgctgtt tgcggagagg gggcctgcca
     5341 gcctgaccca cgtgggcgcg tgatcgctgg gcttctcctg gtcgcgcacc cagcggtcga
     5401 cgccgccttc tcgcagcctc gctgacgcct cggcattcag cagcaggtga tcgatgcgca
     5461 ggccggcgtt gcgctgccag ctctgccgga aatagtccca gaaggtgtag atgcgctctt
     5521 cggggtacag cttgcgcagc gcgtcggtcc agccctgggc gagcaggcgc tggtagcact
     5581 cgcggctctc gggctgcagc agtgcgtcct tgagccacga gcgcgggttg taaatgtcga
     5641 agtcggtcgg cacgacgttg tagtcgccgc acagcaccac cggccgcccg acgccgtaca
     5701 gcgtcgccgc gtgctcgatc agccgctcga accagcgcag cttgtaatcg aacttggggc
     5761 ccggctgggg attgccgttc ggcaggtaca ggcagccgat gatgaactcg ccgacctcgg
     5821 cctccacgta gcggctgtgt tcgtcgtcgg ggtcgccggg cagggcgcgg cgcacctcga
     5881 ccggcttgat gccgcgggtg aggatcgcga cgccgttcca cgagcgttgc ccgtgccaga
     5941 tcgcctcgta cccggccgcg cggatgtcga ggatcgggaa gccctcgctg acggccttga
     6001 gttcctgcag gcaggcgacg tcgggctgct cgcgctccag ccagcgcagc aggtgcggca
     6061 gccgggcacg gattccgttg acgttgtacg tcgcgattcg ggtcgtggtg gtgctcatcc
     6121 agccagttta ctggttctcg agcgtctggc agctgccgcg ggcgcacgcg cggaaatcgg
     6181 ccagccagcc cgccagcatg ttgcgcacgc tggcccaggc cgcgttgtcg tgctggctgg
     6241 acagctgcaa aggatcggtc ctgaggtcgt agaactcttc gctgccgagc ccgtccttcc
     6301 acgacacgta gatctggtcg ggcggtgtcg cgcggctgag gttggtcgtg cgaacggcgc
     6361 tgaacggcgc acgtggcggc actcgatcgg tctctacgtt gccgagaaac tccacgagga
     6421 accgcttgcg ccacatggtt tccgctggat tgcgcatcag cggcaacagt gagcggccgt
     6481 ccacccgcaa cccgggttcg atgccggcga agtcggccac cgtggaagcg aggtcgttgt
     6541 tcagggcagc ccgtgtggtg gcctgacccg gaaagcccgg cgcacggatg tacaacggca
     6601 cgcggatcga ttcctcgtag cccaggatct tgtcggtcag gcggtgctga ccgtagaacc
     6661 agccgttgtc ggaagtgaag atgaagaccg tgcgcgacca ctcgtccagg cgctgcagcg
     6721 tggtgaccag agagccaacc atgtcgtcga ccgcgcgcag ggacaggagc cgatcacggt
     6781 actggcgttt caggcagccg acgtcgaggg tcg
//
LOCUS       DASPFR010000169         5489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1638263, whole genome shotgun sequence.
ACCESSION   DASPFR010000169 DASPFR010000000
VERSION     DASPFR010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638263"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VD737_04290"
     CDS             complement(<1..308)
                     /locus_tag="VD737_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09810.1"
                     /translation="MFVDRSIDPTADQVRDLRDMPDERPITMVNLLKFRAQARYEDGD
                     AVPCTGREAYARYKHAFTVTVAAVSQAQVVFSGPVQQVFIGRAGQPEADWDEVLVVRY
                     P"
     gene            complement(314..1219)
                     /locus_tag="VD737_04295"
     CDS             complement(314..1219)
                     /locus_tag="VD737_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HXW09811.1"
                     /translation="MLQTFDERNRNLIVNVAGELTHRDQARVSPFDSAVQGGDAVWEG
                     LRLYRGRIFRLEQHLDRLVNSAKALAFAQIPSRAAITTEIRRTLAANDMFDGVHIRLT
                     LTRGVKITSGMDPRLNQHGPTLIVLAEHKPPVYSKAGLSLVTSSVRRFPPDCLDPKIH
                     HNNLLQSILAKIEANVAGADDALMLDMRGFIAETNATHVFVVERGRVRTPRLVACPEG
                     ITRATVLELCAQHGIPHEEADLTLAQAYAADEVFCTGTMGELAAVTSVDGRTVGDGRP
                     GPTVVRLSALFRELTERDGTVVVEE"
     gene            complement(1231..1980)
                     /locus_tag="VD737_04300"
     CDS             complement(1231..1980)
                     /locus_tag="VD737_04300"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09812.1"
                     /translation="MAEPDDDVLRLAMWSGPRNISTAFMRSWGNREDTVVVDEPFYAH
                     YLEVTGIEHPGREEVLAHHECDWRRVVESLLAPLPPGVRVLYQKQMSHHLLPSIGRDW
                     LGHMTHAFLIRDPRSMIASLADRLESFDLLATGLPQQVEIFEFELRRTGRSPPVLDAA
                     DLLRDPPAMLRRLCAALGVPYSDRMLWWPPGRRDTDGIWARHWYDRVERSTGFEPLPS
                     TTPDRLPPRLAAIEAQCRPLYDRLREHRLRA"
     gene            complement(2020..2820)
                     /locus_tag="VD737_04305"
     CDS             complement(2020..2820)
                     /locus_tag="VD737_04305"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXW09813.1"
                     /translation="MHRRSLRHAWPALLALSLAATAALAATGLLKVAADEAAIDAALA
                     SPDRPAADREQDERRHAKEVLTFLGVRPGWRVLDAFSAGGYNTELLARAVGVKGQVIA
                     YNNPPYAKFAAKGIADRYKGERLGNVRQVTAEVDELELAPQSLDGVLFVMSYHDAYWR
                     PKDGSFDRTDPARLLSKLYEALKPGGVVVVQDHVAKAGGDPAVDADKLHRIDPALVRR
                     DFEKAGFTFDGSSDVLAHPDDDHTRLVFDEAIRGRTDQFVYRFRKPMA"
     gene            complement(2861..3799)
                     /gene="gcvA"
                     /locus_tag="VD737_04310"
     CDS             complement(2861..3799)
                     /gene="gcvA"
                     /locus_tag="VD737_04310"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HXW09814.1"
                     /translation="MPRSLPPLTSLRAFEAAARSLSFTKAARELHVTPAAISHQIRGL
                     EQFLGVTLFQRTSRRLELTRDGQLAAEHFREAFERIARGVQALRQQGREGELVIGVTP
                     SFATRWLLPRLPSLADELPQVRVRLVTSTAPVDFDRDDIDLAVRFGRGGFDSVVTDEL
                     FNEVVAPVASPAFLRKHPLRRPADLARVRLLQDDSMRRAGRPPSWTEWLRAAGEPGLE
                     IECGLVVDDSHLALQAACDGQGVALGRLAYAVHDVAQGRLVLPFSQVIELDLHYHLIL
                     PEARAQEPLIARFREWLLRQAEDFRGVLAGHARKAA"
     gene            3879..4631
                     /gene="otsB"
                     /locus_tag="VD737_04315"
     CDS             3879..4631
                     /gene="otsB"
                     /locus_tag="VD737_04315"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HXW09815.1"
                     /translation="MRIHKLHPGLLPAATALFLDVDGTLIEFGPAPDDVEVDAATLKF
                     LQDASDTLDGALALISGRSLAQLDALFGYARFPAAGLHGLERRDARGRLHVHAAEPLP
                     QSLVDELGEIAERHPGVLLEDKGRGVALHYRQAANLQEMLEREVHALARRHGGPDLQV
                     QPGAYVLELKPSGITKAHAIEAFMQEPPFSGRTPLFAGDDLTDLHGFDAVERLGGVSI
                     AVGPRVSAMIPADTPTALRALLEEFLAREAAA"
     gene            4648..>5489
                     /locus_tag="VD737_04320"
     CDS             4648..>5489
                     /locus_tag="VD737_04320"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HXW09816.1"
                     /translation="MSRLVAVSNRVTVPRRAATPGGLALGVHAAMQARGGFWFGWSGE
                     IADREPDAARVVIRDKITYGTVELTQADHDLYYLGFCNGAIWPLFHYFLDAVHYHDEQ
                     YEAYLRVNRTFARQLTPHLEPDDLIWVHDYHLIPLAQELRTLGAQQPIGFFLHIPFPH
                     VEALRALPVYGELMRALLDFDLVAFQTERDLECFRSAVFDLWGPEAIAGDGSVVACGR
                     RTWLEHIPIGVDVDAIQKAAVSAQSSEAVQRMVSGLVGRRCVIGVDRLDYSKGLVERF
                     DAYAR"
BASE COUNT          816 a         1899 c         1926 g          848 t
ORIGIN      
        1 gggtagcgca ccacgagcac ttcatcccag tcggcctcgg gctggccggc gcggccgatg
       61 aacacctgct gcacgggccc ggagaacacg acctgcgcct ggctcaccgc ggcgaccgtc
      121 accgtgaacg cgtgcttgta gcgcgcatag gcctcgcgac cggtgcacgg caccgcgtcg
      181 ccgtcttcgt agcgcgcctg cgcgcggaac ttgagcaggt tcaccatcgt gatcggtcgc
      241 tcgtccggca tgtcgcgcag gtcgcgtacc tggtcggcgg tcgggtcgat gctgcggtcg
      301 acgaacatgt gcactactcc tcgacgacga cggtgccgtc gcgttccgtc aattcccgaa
      361 acagcgccga cagccgcacg accgtcggcc cggggcgccc gtcgccgacc gtgcggccgt
      421 cgacggacgt gaccgcggcg agttcgccca tcgtgccggt gcagaacacc tcgtccgcgg
      481 cataggcctg cgccagcgtc aggtcggctt cctcgtgagg gatgccatgc tgcgcgcaga
      541 gctcgagcac ggtcgcacgc gtgatgcctt ccgggcacgc cacgagccgc ggcgtccgca
      601 cgcgcccccg ttcgaccacg aacacgtgcg tcgcgttggt ctcggcgatg aatccgcgca
      661 tgtccagcat cagcgcgtcg tcggcgcccg cgacgttggc ctcgatcttg gcgaggatcg
      721 actggagcag gttgttgtgg tgaatcttcg ggtcgagaca gtcgggcgga aaccgccgga
      781 cgctgctggt gacgaggctc aagcccgctt tcgaatacac cggtggcttg tgctcggcca
      841 gcacgatcag cgtcgggccg tgctgattga gccgcggatc catgcccgag gtgatcttca
      901 cgccgcgggt cagcgttagt cggatgtgca cgccgtcgaa catgtcgttg gcggcgagcg
      961 tgcgccggat ttccgtcgtg atcgcggccc gcgacgggat ctgcgcgaac gcgagcgcct
     1021 tggccgagtt gaccagccgg tccaggtgct gctccagccg gaagatgcgc ccccggtaga
     1081 ggcgcaaccc ctcccagacg gcgtcgccgc cctgcaccgc cgagtcgaac gggctcacgc
     1141 gcgcctggtc gcgatgcgtg agttcgccgg cgacgttgac gatcaggttg cggttgcgtt
     1201 catcgaaggt ctgcagcacg tcgagggtcc tcaggcccgg agccggtgct cgcggagccg
     1261 gtcgtagagc ggtcggcact gggcctcgat cgccgcgagc cgcggcggca aacggtccgg
     1321 cgtggtggac ggcaggggtt cgaagccggt cgatcgctcg acgcggtcgt accagtgccg
     1381 ggcccagatg ccgtcggtgt cgcgtcgtcc cggtggccac cacagcatgc gatcggagta
     1441 cggcacgccg agtgccgcac aaagcctccg caacatcgcg ggcggatcgc gcaggaggtc
     1501 ggcggcgtcg agcacgggcg gcgaccgccc cgtgcgtcgc agctcgaact cgaagatctc
     1561 gacctgctgc ggcaggcccg tggcgagcag gtcgaacgat tccagcctgt cggcgagcga
     1621 ggcaatcatc gaccgggggt cccggatcag gaacgcgtgc gtcatgtgcc cgagccagtc
     1681 gcgcccgatc gacggcagca ggtgatgcga catctgcttc tggtagagca cgcgcacgcc
     1741 gggtggcagg ggcgcgagca gggactcgac cacgcgccgc cagtcgcact cgtggtgcgc
     1801 caggacttcc tcgcggccgg gatgctcgat gcccgtgacc tcgaggtagt gcgcgtagaa
     1861 cggctcatcg acgacgacgg tgtcctcgcg gttgccccac gaacgcatga acgccgtcga
     1921 gatgttgcgc gggcccgacc acatcgcgag ccgcagcacg tcgtcgtcag gctcagccat
     1981 ccgccccttc cgcgcggccg ctgccgcgcg ccgggcatct taggccatgg gcttgcggaa
     2041 acgatagacg aactggtcgg tcctgccgcg gatggcctcg tcgaagacca gcctggtgtg
     2101 gtcgtcgtcc gggtgcgcca gcacgtcgga cgagccgtcg aacgtgaacc cggctttctc
     2161 gaagtcgcga cgcacgagtg ccgggtcgat gcggtgcagc ttgtccgcat ccaccgcagg
     2221 atcgccgccg gccttggcga cgtggtcctg cacgacgacg accccgcccg gcttgagcgc
     2281 ctcgtacagc ttcgagagca gtcgcgccgg atccgtgcgg tcgaagctgc cgtccttcgg
     2341 gcgccagtag gcgtcgtgat agctcatcac gaacaggacg ccgtcgagcg actggggcgc
     2401 gagctcgagc tcgtcgactt ccgccgtgac ctgtcggacg ttgccgagcc gctcaccctt
     2461 gtagcggtcg gcgatgccct ttgcggcaaa cttcgcgtac ggcggattgt tgtaggcgat
     2521 gacctggccc ttgacgccga ccgcgcgcgc aagcagttcg gtgttgtagc cgccggccga
     2581 aaaagcgtcg agcacgcgcc agcccggcct gacgccgagg aacgtgagca cttccttcgc
     2641 gtgccgtcgc tcgtcctgct cgcgatcggc cgcgggccga tcgggactcg ccaatgcggc
     2701 gtcgatcgcc gcctcgtcgg cggccacctt caacagcccg gtcgcagcca gcgccgcggt
     2761 ggccgcgagc gaaagggcca gcagggccgg ccaggcgtga cgcagcgagc gacggtgcat
     2821 gcgagtctcc tgcaatgggg gcgcgacgcg gcccgcgcgg tcaggcggcc tttcgggcgt
     2881 gtccggcgag cacgccgcgg aagtcctctg cctgtcgcag cagccactcg cggaagcggg
     2941 cgatcagcgg ctcctgcgcg cgggcctcgg gcaggatcag gtggtagtgc aggtcgagtt
     3001 ctatgacctg cgagaacggg agcaccagcc gtccctgcgc cacgtcgtgg acggcgtacg
     3061 ccagccggcc gagggcgacg ccctgtccgt cgcacgctgc ctgcaaggcg aggtggctgt
     3121 cgtcgacgac gaggccgcat tcgatctcga gccccggttc gccggcggcg cgcagccact
     3181 cggtccacga aggcggccgg cccgcgcgtc gcatggagtc gtcctgcagc aggcgcacgc
     3241 gcgccaggtc cgccggacgt cgcaacgggt gcttgcgcag gaacgcaggc gacgcgaccg
     3301 gcgcgacgac ttcgttgaac agctcgtcgg tgacgaccga atcgaagccg ccgcgtccga
     3361 aacgcaccgc caggtcgatg tcgtcgcggt cgaaatcgac gggcgccgtg ctcgtgacga
     3421 ggcgaacgcg gacctgcggc agttcgtccg cgagggacgg cagccgtggc agcagccagc
     3481 gcgtggcgaa ggaaggcgtc acgccgatca cgagctcgcc ttcgcgcccc tgctggcgca
     3541 gcgcctgcac gccgcgcgcg atgcgttcga aggcctcgcg gaaatgctcg gccgccagct
     3601 ggccgtcacg cgtcaactcg agccggcggc tcgtgcgctg gaacagcgtc acgccgagga
     3661 actgctccag gccgcggatc tggtggctga tcgccgccgg ggtgacgtgg agttcccgcg
     3721 cggccttggt gaaactgagc gaacgggccg ctgcctcgaa cgcacgcagc gacgtcagcg
     3781 gcggcagcga acggggcatg gatgagttcc tttcaacgac gaaatgatgt tttctagctt
     3841 acggggcatc cgtattcgac cctatgctcg gcggattcgt gaggatacac aaactgcacc
     3901 cagggctgct ccccgcggcc accgcgctgt tcctcgatgt cgatggcacg ctgatcgaat
     3961 tcggcccggc gcctgacgat gtcgaggtcg atgccgccac gctgaaattc ctgcaggacg
     4021 cgtccgacac gctcgatggg gcgctcgcgc tgatcagcgg gcgctccctg gcgcaactgg
     4081 acgccctgtt cggctatgcg cgcttccccg cggcgggact gcacggtctc gagcgtcgcg
     4141 acgcgcgagg acggctgcac gtgcacgccg cggagccgtt gccgcagtcg ctggtcgacg
     4201 agctcggcga gatcgccgag cgccacccgg gcgtgctgct cgaggacaag ggccgcggcg
     4261 tggcgctgca ctaccgccag gcggcgaacc tgcaggaaat gctcgagcgc gaggttcatg
     4321 ctctggcacg acgacacggc ggccctgacc tgcaggttca gccgggcgcc tatgtgctcg
     4381 aactcaagcc ctcgggtatc acgaaggccc acgcgatcga ggccttcatg caagaaccgc
     4441 cgttttccgg acgcacgccg cttttcgccg gcgacgacct gaccgacctg cacggcttcg
     4501 atgccgtcga gcgcctgggc ggtgtctcga tcgcagtggg accgcgcgtg tcggcgatga
     4561 tcccggcgga cacgccgacc gcgcttcgcg cgctgctcga ggagtttctc gcgcgcgagg
     4621 ctgcggcatg acccgggcag acgggtcatg agccgcctcg tcgccgtctc gaaccgcgtg
     4681 accgtgccgc gccgcgcggc gacgccgggc ggcctcgcgc tcggcgtgca cgccgcgatg
     4741 caggcgcgcg gcggcttctg gttcggctgg agcggcgaga tcgcggaccg cgagcccgat
     4801 gccgctcgag tcgtgatccg cgacaagatc acctacggca ccgtcgagct gacgcaggcc
     4861 gaccacgacc tgtattacct cgggttctgc aacggcgcca tctggccgct gttccactac
     4921 ttcctggacg ccgtccacta ccacgacgag cagtacgaag cgtacctgcg cgtcaaccgg
     4981 acgttcgccc gccagctgac gccgcacctc gagcccgacg acctgatctg ggtgcacgac
     5041 taccacctga taccgctcgc gcaggaactc cgcacgctcg gcgcgcagca gccgatcggg
     5101 ttcttcctgc acatcccgtt tccgcacgtc gaggcgctgc gcgcgctgcc ggtctatggc
     5161 gagctgatgc gggcgctgct cgacttcgac ctcgtcgcct tccagaccga gcgcgatctc
     5221 gagtgcttcc gctcggccgt gttcgatctc tggggtccgg aggcgatcgc cggcgacggc
     5281 tccgtggtcg cctgcggccg ccgcacctgg ctggaacaca tcccgatcgg cgtcgacgtg
     5341 gatgccatcc agaaggccgc ggtctcggcg cagtcctcgg aggccgtgca gcgcatggtg
     5401 tcgggcctcg tcggccggcg ctgcgtgatc ggggtggacc ggctcgacta cagcaagggc
     5461 ctggtcgaga ggttcgacgc gtacgcccg
//
LOCUS       DASPFR010000170         2020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1516342, whole genome shotgun sequence.
ACCESSION   DASPFR010000170 DASPFR010000000
VERSION     DASPFR010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516342"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1783
                     /locus_tag="VD737_04325"
     CDS             <1..1783
                     /locus_tag="VD737_04325"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HXW09817.1"
                     /translation="LSTGGTATDVTDVIHPDNRDMAVRAVRAVGLDVGGVDFLSKDIT
                     LSYRDIGGGICEVNAAPGFRMHVAPSEGTPRDAAGPVIDMLFPPGTPSRVPIAALTGT
                     NGKTTTARMLAHITKMAGYSPGLTTTDGVYIDGRRTVAGDMTGPVSARMVLADPQIDM
                     AILETARGGLLRAGMGVSEVNVGAVLNVQSDHLGLKGIDTLEQLAEVKRVVVEVATDC
                     AVLNADDPHVLRMAGHTEAKNICYVTMNPQHELVREHIRAGGRACALEAGVNGQMITL
                     YDRGGHIPLVWTHLIPATLEGRAMHNVQNAMFAAAMAFSLGIKLDAIRHGLRTFDSTF
                     FQAPGRMNVFNEHPFKVLFDYGHNAHAVAALADLAARLDCTGRRIVVLAGPGDRRDED
                     LRAIAQAVAGRFDHYICRRDDDLRNRAPDEVPRIQSAALQESGVPASAISVIPDEQEA
                     IDAALNMGQPGDLLLVFADALTRSWKQIIKFRSPTSPASAGEFARNAGDGSYVQRPTG
                     EAARSAGEGSGIGGFISATAPAPAGEVARSAGEGRAFAAPQAARPVLAPRDPEPVEPS
                     FAGEIAKELHGLIRDERGVRLAPEAED"
     gene            1785..>2020
                     /locus_tag="VD737_04330"
     CDS             1785..>2020
                     /locus_tag="VD737_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09818.1"
                     /translation="MTPPLPFADSRRLTGANLFFGQPGAVLETAGLEADAALLDGWRS
                     RVDRARAHLGWPPALAVSARRHAGGTSLALAAPA"
BASE COUNT          305 a          751 c          702 g          262 t
ORIGIN      
        1 cctttccacc ggcggcaccg ccaccgacgt gaccgacgtg atccacccgg acaaccgcga
       61 catggcggtg cgtgcagtgc gcgccgtcgg actcgacgtc ggcggcgtcg atttcctcag
      121 caaggacatc accctgagct accgcgacat cggcggcggc atctgtgagg tcaacgccgc
      181 gccgggcttc cgcatgcacg tcgcgccgag cgagggcacg ccgcgcgatg ccgccggacc
      241 cgtgatcgac atgctgttcc cgccggggac gccgtcgcgc gtgccaatcg ccgcgctgac
      301 gggcaccaac ggcaagacca cgaccgcccg catgctcgcg cacatcacca agatggccgg
      361 gtactcgccg ggactcacca cgaccgacgg cgtctacatc gacggtcggc gcaccgtggc
      421 cggcgacatg acgggcccgg tctcggcacg catggtgctg gccgacccgc agatcgacat
      481 ggcgatcctc gagaccgcac gcggtggcct gctgcgggcc ggcatgggcg tttccgaggt
      541 caacgtcggc gcggtgctga acgtgcagag cgatcacctc gggctcaagg gcatcgacac
      601 gctcgagcag ctggccgagg tcaagcgcgt ggtcgtcgag gtcgcgaccg actgcgcggt
      661 gctcaacgcc gacgacccgc acgtgctgcg catggcgggc cacaccgagg cgaagaacat
      721 ctgctacgtg acgatgaacc cgcagcacga gctcgtgcgc gagcacatcc gcgcgggcgg
      781 gcgcgcctgc gcgctcgagg caggcgtcaa cgggcagatg atcacgctgt acgaccgcgg
      841 cggccacatc ccgctggtct ggacgcacct gattcccgcg acgctcgaag gccgcgcgat
      901 gcacaacgtg cagaacgcga tgttcgcggc cgccatggcg ttctcgctcg gcatcaagct
      961 cgacgcgatc cgccacgggc tgcgcacgtt cgactcgacg ttcttccagg cgcccgggcg
     1021 catgaacgtg ttcaacgagc acccgttcaa ggtgctgttc gactacggcc acaacgcgca
     1081 cgcggtcgcc gcactcgcgg acctggccgc gcgcctcgac tgcaccggcc ggcgaatcgt
     1141 cgtgctcgcg ggcccgggcg accggcgcga cgaggacctg cgcgcaatcg cacaggccgt
     1201 ggccggacgg ttcgaccact acatctgccg gcgcgacgac gacctgcgca accgcgctcc
     1261 cgacgaggtg ccgcggatcc agtcggcggc gctgcaggaa agcggcgtgc ccgcgagcgc
     1321 catctcggtc atcccggacg agcaggaagc gatcgacgcc gcgctgaaca tgggccagcc
     1381 cggcgacctg ctgctggtct tcgccgatgc gctgacgcgt tcgtggaagc agatcatcaa
     1441 gttccgttcc cccacgtctc cggcgtcagc cggagagttc gcccggaacg cgggtgacgg
     1501 ctcctacgtc cagcgtccga cgggagaggc tgcgcgcagc gcaggtgagg gctccggcat
     1561 cggtggcttc atctctgcca ccgcgcccgc gccagcgggc gaggtcgcgc gcagcgcagg
     1621 tgagggccgc gcctttgccg ccccccaggc cgcccgaccc gtcctcgcgc cacgcgaccc
     1681 cgagcccgtc gagccgtcgt tcgcgggcga gatcgccaag gaactgcacg ggctgatccg
     1741 cgacgagcgc ggtgtgcggc tcgcgccgga agccgaagac tgacgtgacg ccgccgctgc
     1801 cgttcgcgga ctcccgccgg ctgacgggcg caaacctgtt cttcggccag cccggcgccg
     1861 tgctcgaaac ggccgggctc gaggccgacg ccgcgttgct cgacggatgg cgctcgcggg
     1921 tcgatcgggc gcgcgcccac ctcggctggc cgccggccct cgccgtgagc gcgcggcggc
     1981 acgcgggcgg cacgtcgctc gcgctggccg cccccgcgga
//
LOCUS       DASPFR010000171         4281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_295340, whole genome shotgun sequence.
ACCESSION   DASPFR010000171 DASPFR010000000
VERSION     DASPFR010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295340"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            423..746
                     /locus_tag="VD737_04335"
     CDS             423..746
                     /locus_tag="VD737_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09819.1"
                     /translation="MQQGRKAEETRVFEALKEKSRLSADLEAAEETIGSQQQEIAKLR
                     EAVRVRSECIKELCACLAEATPWIDPIMIGPVTHTGEAARNKLLERIGAALTWAGHKP
                     PAPAT"
     gene            complement(874..1731)
                     /locus_tag="VD737_04340"
     CDS             complement(874..1731)
                     /locus_tag="VD737_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXW09820.1"
                     /translation="MEPKHNLSARAWTRIAGAGIVAWGLGACAPTPVVPEGALQARAE
                     LARLQADAKLAPLAPQALTEAEAAVQLAEQTDAEPEVSAHRVYLAQRKVELARALAEA
                     RYAEQERSALTAQLDRARLEAREREAAAARSDAQTARMDADAARMQAAAMQAEMAAMN
                     ARQTDRGMVLTIGDVLFDTGKANLKSGAVTDLDELARFLAKYPDRSVVIEGHTDSVGS
                     QQFNVGLSERRAASVQTYLMRQGIDASRIRTQGMGESVPVASNESAGGRQQNRRVEII
                     VSNPTQASR"
     gene            complement(1731..2150)
                     /locus_tag="VD737_04345"
     CDS             complement(1731..2150)
                     /locus_tag="VD737_04345"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HXW09821.1"
                     /translation="MLHPQYRDAPPRRRTNTAKWLAAVSGAALLAAGCASAPPPTAQI
                     ESARQAIENAERAQAAQYAAPELSQARSKIASADTAVQSEEMEQAARLAAEARADAEL
                     ASARTAAAKAQSANDEIRRANQMIIEEMGRSTTGGTQ"
     gene            2478..2849
                     /locus_tag="VD737_04350"
     CDS             2478..2849
                     /locus_tag="VD737_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09822.1"
                     /translation="MESKRLIVAAAVLGLTLGATGCGRDDDAMDTTGTTDATGTTGTD
                     TTGTTGYGDTTGTGTTTPESDAAMGTTTDPNMPPADPNMPPADPTTPGTTGDTTTTPG
                     TTTDQDQSTTTDTTAPPPPGG"
     gene            complement(2920..3921)
                     /locus_tag="VD737_04355"
     CDS             complement(2920..3921)
                     /locus_tag="VD737_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09823.1"
                     /translation="MHSAIAASLLALVGSMFLPGSHAETRPASTPAAVDYVADGRAPL
                     DQLARRFAELDAKHGWAVETVYAYPDAPDLQIKAWRTAATGPALWVLAGIHGEEPAGP
                     NAIARELDALVDFARSGVPVVVVPLCNPRAYRHNWRYPNTPERDWKRGGYSVGDAEYL
                     LPNLERGDTPRGERAPGPETYALTQFVLRLADRYPPKLVLDLHEDELSTDGGYIYSQG
                     RRAGESPVGLEVVRLLQSAGIPLRRSGKTRFGETIVDGVISRDDQGGPIRDGSIDELL
                     ASPEVFVAGRTVRGPSADTVIVVETPAFAGARLDQRVAAQAAVVRQVATLWRLNDAP"
     gene            complement(3921..>4281)
                     /locus_tag="VD737_04360"
     CDS             complement(3921..>4281)
                     /locus_tag="VD737_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09824.1"
                     /translation="VVTGLLATAMVLMNYSKSLVEGFTFLTLIVTAANLPLYLLCALA
                     LIVLARRGERRLPASMLVLGVIGTAYSVFAFVGLGHEPFLWSLVLGAAGLPVYWALRH
                     RRTVGVASIAPADGRDG"
BASE COUNT          650 a         1498 c         1504 g          629 t
ORIGIN      
        1 gggtcgggac gcaaggtcaa gagtcgcaag caggccatcg ccatcggcct gtccgaggcc
       61 cgacgtgaag gcggaaaggc ccctccgcgc aagaagaagt cgagttccag tcgcaagtcg
      121 agctctgggc gaaagtcgag ccggggtcgc aaatcgagtt cgggccggaa gtcgagttcc
      181 gggcggaagt cggggtcggg tcgcaaatcg agccggggca gccggtccag taggtcgtga
      241 cgcccgcacg ccgtctccgc tgagggcttc ggtaagccca gcgtgccgga gcgctccgac
      301 gcgggtgtcg gcgccacgcg catcgcgcct gcgccacggt gacgaagtgg ccgggctcgc
      361 gtccaaagca ggtggaccgg tgcgcaccgt aagaggcccg gccgcgatcg atcgggtatc
      421 cagtgcagca gggccgcaag gccgaggaga cgcgcgtgtt cgaagccctg aaggagaaaa
      481 gccggctttc cgccgacctc gaggcagccg aggagaccat cgggtcgcag cagcaggaga
      541 tcgcgaagct gcgcgaggcc gtgcgcgtcc gcagcgaatg catcaaggaa ctctgcgcct
      601 gcctggcgga agcgaccccc tggatcgacc cgatcatgat cggcccggtg acgcacaccg
      661 gcgaagccgc gcgcaacaag ctgctggagc gcatcggcgc ggccctgacg tgggccggac
      721 acaagccccc ggcaccagcc acctagcggt tcgtccagct cgccgcgacg cgccggctcg
      781 cgcagccgcg accatgcggc gtcgcggctg atcgtccttc gggcggcggg tcgaacccgg
      841 agcccgagga cggccatccg ccaggccgag gcgttacctc gacgcctgcg tcggattgct
      901 gacgatgatc tcgacgcgac ggttctgctg acgcccgccg gccgattcgt tcgacgcgac
      961 cgggacgctc tcgcccatgc cctgggtcct gatccgtgac gcgtcgatgc cctggcgcat
     1021 caggtacgtc tgcacggacg ccgcccggcg ttcggacaag ccgacgttga attgctggct
     1081 gccgacgctg tccgtgtgcc cctcgatcac gaccgaccga tcggggtact tggccaggaa
     1141 tctcgcaagc tcatccaggt cggtcacggc gccgctcttg aggttggcct tgccggtgtc
     1201 gaacagcacg tcaccgatgg tcagcaccat gccgcggtcc gtctggcgcg cgttcatcgc
     1261 cgccatctcg gcctgcatgg ccgcagcctg catgcgtgcg gcgtcggcgt ccatgcgcgc
     1321 cgtctgcgcg tcgctgcgcg cggccgcggc ttcgcgttcg cgtgcttcga ggcgggcgcg
     1381 gtcgagttgc gccgtcagcg cggaacgctc ctgctcggcg taacgcgctt cggccagggc
     1441 gcgtgcgagt tcgaccttgc gctgcgcgag gtagacccgg tgtgcgctca cttcgggctc
     1501 cgcgtccgtc tgctccgcga gctgcacggc ggcctcggcc tccgtcagcg cctgcggcgc
     1561 gagaggtgcc agctttgcgt cggcctgcag gcgcgccagt tcggcgcgag cctgcagggc
     1621 cccttcggga accaccggcg tcggcgcgca ggcgcccagt ccccacgcga cgattccagc
     1681 gcccgcgatg cgcgtccagg cacgtgcaga aaggttgtgc ttcggttcca tcactgggtc
     1741 cctcccgtgg tcgagcgccc catttcctcg atgatcatct ggttcgcgcg gcggatctcg
     1801 tcgttggcgg actgtgcctt ggccgctgcg gtgcgcgcgg aggcaagctc ggcatcggcg
     1861 cgggcttctg cggccagtcg cgcggcctgt tccatctcct cgctctgcac ggcggtgtcc
     1921 gccgacgcga tcttggagcg cgcctggctc agctccggtg cggcgtactg ggcggcctgc
     1981 gcgcgctcgg cattctcgat ggcctgcctg gccgattcga tctgcgcggt cggcggtggc
     2041 gcggaggcac agcccgccgc gagcagcgca gcgcccgata ccgccgccag ccacttggcg
     2101 gtgttcgtac gtcgtcgtgg tggagcgtcg cgatattggg gatgcagcat tgttcacctc
     2161 tgtccttgtc gggcctcagc tggcccaccg gttcgcccaa tatgaccact cttggcgcat
     2221 ggcgctgtcc gtcgggcagc gcggcggtgt gcgtgtttcg ccctacgtgg gctcgacggc
     2281 tccccaagcg aagcgcggcg aacgaatggc cggcgctcaa ccgagcgcgc atttgccgcg
     2341 ccctgacgca agcgtgctgc gtctcgcttg gaagtaggat aggtccgcgt gtttttggct
     2401 cgcttgtctg gaagattcgc atcggcgagt ggcggatcgt ccgcacgctc cacaaacgga
     2461 cgagcggaga ggcacacatg gagagcaaga gactgatcgt tgccgccgcc gtgctgggac
     2521 tgacgctcgg cgcgacaggc tgcggcaggg acgacgacgc gatggacacg accggcacca
     2581 cggatgcgac gggcacgacc ggcacggata cgacgggcac gaccggttac ggcgatacca
     2641 ccggcacggg gacgacgact cctgagtccg acgccgccat gggcacgacc accgacccga
     2701 acatgccgcc ggcggatccg aacatgccgc cggcagatcc cacgactccg ggcacgaccg
     2761 gcgataccac cacgacgcct ggcacgacga ccgaccagga tcagagcacg actaccgata
     2821 cgacggcacc gccgccgccc ggcggctgac gctcccgcac aggcgaaccg cgcgcgtcgg
     2881 gcgcctggcg cccggcgcgt gcgtgcgtgt cgccgatcct catggggcgt cgttgagccg
     2941 ccacagggtc gcgacctggc gcacgacggc ggcctgcgcg gccacgcgct ggtcgagacg
     3001 cgcgccggca aacgcgggcg tctcgacgac gatcaccgtg tccgcggacg gaccgcggac
     3061 cgtgcgaccg gcgacgaaca cctccgggct cgcgagcagt tcgtcgatcg acccgtcgcg
     3121 gatgggcccg ccctggtcgt cgcggctgat cacgccgtcg acgatggtct cgccgaagcg
     3181 ggtcttgccg gaccgtcgca gcggaatacc ggcggactgc agcaggcgca cgacctccag
     3241 gccgaccggg ctttcgccgg cgcgacgtcc ctgtgaatag atgtagccgc catcggtcga
     3301 gagctcgtcc tcgtgcaggt cgagcacgag cttcggtgga taccgatcgg cgaggcgcag
     3361 cacgaactgc gtcagcgcgt acgtctcggg tcccggcgcc ctttcgccgc gaggcgtgtc
     3421 gccgcgctcg aggttcggca gcaggtactc cgcgtcgccc acgctgtagc cgccgcgctt
     3481 ccagtcccgc tcaggcgtgt tcggatagcg ccagttgtgg cggtacgccc tgggattgca
     3541 gagcggcacg accacgaccg gaacgccgga ccgcgcgaag tcgaccagcg cgtccagttc
     3601 gcgcgcgatc gcgttcggcc ccgcgggttc ttcgccgtgg atgccggcca gcacccagag
     3661 cgcaggcccc gtcgcggccg tgcgccaggc cttgatctgc aggtccggcg catcgggata
     3721 ggcatagacc gtctcgacgg cccacccgtg cttcgcgtcg agctcggcga aacggcgtgc
     3781 cagttgatcg agcggcgcgc ggccgtcggc gacgtagtcg accgcggccg gtgtggatgc
     3841 cggtcgcgtc tccgcgtgtg agcccggcaa gaacatcgaa ccgaccaagg ccagcaggct
     3901 tgcggcgatc gcagagtgca tcagccatcc ctcccatccg ccggggcaat cgaggccacg
     3961 ccgaccgtgc gtcggtgccg caacgcccag tacaccggca ggccggccgc gcccagcacc
     4021 agcgaccaca ggaacggctc gtgtccgagg ccgacgaacg cgaagaccga gtacgccgtg
     4081 ccgatcacgc cgagcacgag catgctcgcg ggcaagcgac gctccccgcg tcgtgcgagc
     4141 acgatgagcg ccaacgcgca gagcaggtag agcggcaggt tcgcggccgt gacgatcagc
     4201 gtcaggaacg tgaatccctc gaccagcgac ttgctgtagt tcatcagcac catcgcggtg
     4261 gcgagcaggc cggtgacgac c
//
LOCUS       DASPFR010000172         6236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1393065, whole genome shotgun sequence.
ACCESSION   DASPFR010000172 DASPFR010000000
VERSION     DASPFR010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6236
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393065"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1495)
                     /gene="sufB"
                     /locus_tag="VD737_04365"
     CDS             complement(50..1495)
                     /gene="sufB"
                     /locus_tag="VD737_04365"
                     /inference="COORDINATES: protein motif:HMM:NF008773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HXW09825.1"
                     /translation="MSSPQADLDALVARKYRHGFVTDVESDTLPPGLDEDVVTIISRK
                     KREPEFMLQWRLKALRHWHTMREPDWAHLRIAPIDYQAISYYSAPRRKDGPKSLDEVD
                     PKLLATYEKLGVPLHERARLAGVAVDAVFDSVSVATTFKEKLSKAGVIFCSFSDAVLN
                     HPALVEQYLGSVVPYTDNFFATLNSAVFSDGSFVYVPKGVRCPMELSTYFRINAANTG
                     QFERTLIVADEGSHVSYLEGCTAPMRDENQLHAAVVELVALADANIKYSTVQNWYPGD
                     ENGVGGIYNFVTKRGECRGANSRISWTQVETGSAITWKYPSCVLTGENSVGEFYSVAL
                     ANHYQQADTGTKMIHIGRNTRSTIVSKGISAGRGQNTYRGLVKVLPSAEGARNHTQCD
                     SLLMGDKCGAHTFPYMEIKNPSAIVEHEATTSKIADDQLFYCRSRGVSEEDAVNMIVN
                     GFCKAVFKELPMEFAVEAQNLLSVSLEGSVG"
     gene            complement(1501..1989)
                     /locus_tag="VD737_04370"
     CDS             complement(1501..1989)
                     /locus_tag="VD737_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HXW09826.1"
                     /translation="MLRISKLTDYGTVVLACLAASPAHRLTATEVAERTRLGLPTVSK
                     LLKSFHRAGLLTSTRGARGGYLLARPAAAISAAAIIDAIEGPVAITECSGQHSTCAHE
                     VSCSTGSAWQRINGAIRRSLDEISLAQLSGQEAVAVWRPHLSEVARPAAPPRGPGRIQ
                     DR"
     gene            2124..2981
                     /locus_tag="VD737_04375"
     CDS             2124..2981
                     /locus_tag="VD737_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXW09827.1"
                     /translation="MSSPTSSTVSVPPVACRDSIAVLRNVHYMVDSGKTVLEGLDIDI
                     CRGAITAIMGPSGTGKTTLLKLLTGQIRADRGEVVVDGQDLAQLNRKQIYALRRRMGM
                     LFQNGALLTDLDVFENVAFPLREHARLPERLIRTIVLSKLHAVGLRGAASLLPQQLSG
                     GMARRVALARAIAMDPDILIYDEPFAGLDPISMGVVLRIIKSLNDALGITSIVVTHDV
                     REISQIADSSYILSGGRVVASGTTRELQEHPSPVVHQFVEGLADGPVPFHYPAPDYHA
                     QLLGGQVFD"
     gene            2981..3754
                     /gene="mlaE"
                     /locus_tag="VD737_04380"
     CDS             2981..3754
                     /gene="mlaE"
                     /locus_tag="VD737_04380"
                     /inference="COORDINATES: protein motif:HMM:NF033619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HXW09828.1"
                     /translation="MPASLIRELVDGAANLFERAGRITRFGLQLAVLTPTALLRPRSI
                     VDQVYNTGALSLVIVMTCGLFVGMVLGLQGYEMLKRVGSEEYLGTAAALSLLKELGPV
                     VTALLFAGRAGTALAAEIGLMRATDQLAAMDIMAVDPMRRVVAPRFIGGVIAMPLLTA
                     TFITIGIYGAQLIGVQFFGIDQGSFWSQIRGAVELDDVGEGLIKGAVFGVACSLIAVY
                     EGYHAVPTAEGVGRATTRTVVVSAVVTLVLDYLLTALML"
     gene            3772..4281
                     /gene="mlaD"
                     /locus_tag="VD737_04385"
     CDS             3772..4281
                     /gene="mlaD"
                     /locus_tag="VD737_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HXW09829.1"
                     /translation="MRPSRTIDISTGLFVLLGFAAILFLVTQITNREFTLNSTSYRLR
                     ADFENVGGLKPGAPVSMAGVTVGRVENIQYDQKLYKAVVTLRVDGRYDEIPNDSDASI
                     LTSGLLGGQYVGLSPGGSDEYFKDGDQVTFVQDAVVLESLISKYLFNTASKQQTPEPA
                     ADAATAPQE"
     gene            4293..4985
                     /locus_tag="VD737_04390"
     CDS             4293..4985
                     /locus_tag="VD737_04390"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXW09830.1"
                     /translation="MNGIIGTLTVVAVLGAVPLAVASAQAAEKPATAAAVPGPGPQQV
                     IQKVSQDLLRDLDVNRATYKKEPQKLRALVDKHLLPNFDVDYAARLVLGKHWRSASEA
                     QRKRFIDAFYQSLMRNYGDAIVEFQENQLKILPFRGDPASGTATVRTEIMRQGTPVPV
                     NYSMRATPQGWKAWDVTIEGISYVKNYRTDFGAQADKEGIDAVIQRLESQNAAGKPDP
                     NLKAPAKAATKS"
     gene            4996..5301
                     /locus_tag="VD737_04395"
     CDS             4996..5301
                     /locus_tag="VD737_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXW09831.1"
                     /translation="MAAFEIRATSPGRLEAQGAICYDGAARALKAGLDLIPRGQPCTI
                     DLSRVTEADSAGLAVLVEWLATARKRQSSIRYEGIPAQILAVARISDLDDLLLGESG"
     gene            complement(5387..>6236)
                     /locus_tag="VD737_04400"
     CDS             complement(5387..>6236)
                     /locus_tag="VD737_04400"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HXW09832.1"
                     /translation="SSLDRGPARLACPNVALVRGLVISLLAVVALGGCATTSAAKGST
                     ASEEEAAEARAAHNPDPIEGFNRGVYKFNDAIDRHALRPAAVAYRDHTPAWLQKGVGN
                     FFTNLFYPTTIVNQFLQGKLKEGSQDIARFVINTTLGWGGIFDVATGARLPVHDEDSG
                     QTLGRWGVPPGPYLMLPLLGPATLRDAPARIADDFTQPFRWYDAGNERYFSLAVYLVD
                     KRAGLLQLDRLVNEAYDPYAFVRDAYLQRRQYAVYDGDVPQEELEDDSDWAEEALRED
                     EAEEEE"
BASE COUNT         1017 a         2076 c         2097 g         1046 t
ORIGIN      
        1 tgggcgtgca ggtcgacgat gctcagcatt caggtgcctc tcgttgcggt cagccgacgg
       61 agccttcgag gctgaccgac agcaggttct gggcttccac ggcgaactcc atcggcagct
      121 ccttgaacac cgccttgcag aagccgttga cgatcatgtt gacggcgtcc tcctcggaga
      181 cgcctcggct gcggcagtag aacagctggt cgtcggcgat cttcgaggtc gtggcctcgt
      241 gctcgacgat tgccgaggga ttcttgatct ccatgtacgg gaacgtgtgt gcgccgcact
      301 tgtcccccat cagcagcgag tcgcactgcg tgtggttgcg cgcgccctcc gccgacggca
      361 ggaccttcac gagcccgcgg taggtgttct ggccgcggcc cgcggaaatg cccttggaga
      421 cgatcgtcga gcgcgtgttg cggccgatgt ggatcatctt ggtgccggtg tcggcctgct
      481 ggtagtgatt cgccagcgca accgaataga actcgccgac cgagttctct cccgtcagca
      541 cgcagctcgg gtatttccag gtgatggccg acccggtctc gacctgcgtc cagctgatgc
      601 gtgagttggc gccccggcat tcgccgcgct tcgtgacgaa gttgtagatg ccgccgacgc
      661 cgttctcgtc gccggggtac cagttctgca ccgtggagta cttgatgttg gcgtcggcga
      721 gcgccacgag ctcgaccacc gcggcgtgca actggttctc gtcccgcatc ggcgccgtgc
      781 agccctcgag gtagctcacg tggctgccct cgtcggcgac gatcagcgtc cgctcgaact
      841 gcccggtgtt ggccgcgttg atccggaaat acgtcgacag ctccatcggg caacgcacgc
      901 ccttggggac gtagacgaac gagccgtcgg agaacaccgc cgagttcagc gtcgcgaaga
      961 aattgtcggt gtacggcacg acgctgccga ggtactgctc gacgagggcg gggtgattca
     1021 agaccgcgtc ggagaacgag cagaagatca cccccgcttt ggagagtttc tccttgaacg
     1081 tggtcgcgac cgagacgctg tcgaacaccg cgtccaccgc cacgccggcg agccgcgcgc
     1141 gctcgtgcag cgggacgccg agcttctcgt aggtggcgag cagcttcgga tcgacctcgt
     1201 ccaggctctt cgggccgtcc ttcctcctcg gggcggagta gtacgagatc gcctggtagt
     1261 cgatcggggc gatgcgcagg tgcgcccagt cgggttcgcg catcgtgtgc cagtgccgca
     1321 gggccttcag gcgccactgc aacatgaatt ccggctcgcg cttcttgcgc gagatgatgg
     1381 tgaccacgtc ctcgtcgagg ccgggcggca gcgtgtcgga ctccacgtcc gtgacgaagc
     1441 cgtgccggta cttgcgggcg acgagggcat cgaggtcggc ctggggactc gacatgcgcg
     1501 ttacctgtcc tggatgcggc cggggccgcg cggcggagcc gcggggcgcg cgacctctga
     1561 aaggtggggc cgccagacgg cgaccgcttc ctggcccgac agttgcgcca gcgagatctc
     1621 gtcgagcgag cgacggatcg cgccgttgat gcgctgccag gcgctgccgg tgctgcacga
     1681 gacctcgtgt gcacaggtgc tgtgctggcc gctgcactcc gtgatcgcga ccgggccctc
     1741 gatcgcatcg atgatcgcgg cggcactgat cgcggccgcg ggccgggcga gcaggtatcc
     1801 gccgcgcgcg ccgcgggtcg acgtcaacag cccggcgcgg tggaagctct tgaggagctt
     1861 gctcacggtg gggaggccca gccgggtgcg ctcagcgacc tcggtcgcgg tcaggcggtg
     1921 cgccgggctc gcggcgaggc aggcgagcac gaccgtgccg taatcggtca gtttgctgat
     1981 gcgtagcatg ggtgaaatcc aagtgggacg attttagtcc tgattcgacc cggagccaac
     2041 cctggccacg tcgcatccga ggaaaaacaa ccacttctgc taagatccgc gtcttttcgc
     2101 cccaaccgaa cacccggcat accttgagca gccccacttc cagcaccgtc agcgtcccac
     2161 cggtggcgtg tcgcgattcc atcgcggtcc tgcggaacgt ccactacatg gtggactccg
     2221 gcaagaccgt gctggaaggc ctcgacatcg acatctgccg cggcgccatc acggccatca
     2281 tggggccgag cggcacgggc aagaccaccc tgctcaagct gctcaccggg cagatccggg
     2341 ccgaccgcgg cgaggtcgtc gtcgacgggc aggacctggc gcagctcaac cgaaagcaga
     2401 tctatgcgtt gagacgccgg atgggaatgc tgttccagaa cggcgcgctg ctcacggacc
     2461 tcgacgtgtt cgagaacgtc gccttcccgc tgcgcgaaca cgcgcggctg ccggagaggc
     2521 tgatccgcac gatcgtcctc agcaagctgc acgccgtcgg cttgcgcggc gcggccagcc
     2581 tgctgcccca gcaactctct ggcggcatgg cacggcgcgt agcactcgca cgggcgatcg
     2641 cgatggaccc cgacatcctg atctacgacg agccgttcgc cgggctcgac ccgatctcga
     2701 tgggcgtggt gctgcgcatc atcaagtcgc tcaacgacgc gctgggcatc acgagcatcg
     2761 tcgtcaccca cgacgtgcgc gagatctcgc agatcgccga ctcgagctac atactctctg
     2821 gcggccgcgt ggtcgccagc ggcacgacgc gcgagctgca ggagcatccc tcgccggtcg
     2881 tgcaccagtt cgtggagggc ctcgcggacg gcccggtgcc gttccattac ccggctccgg
     2941 attaccacgc gcagttgctc ggcggccagg tgttcgactg atgccggcgt cactgatccg
     3001 cgaactggtc gacggcgccg cgaacctgtt cgaacgcgcg ggacggatca cgcgcttcgg
     3061 gctgcagctc gcggtgctga cgccgacggc gctgctgcgg ccacgttcga tcgtcgacca
     3121 ggtctacaac acgggcgcgc tctcgctcgt catcgtcatg acctgcggcc tgttcgtcgg
     3181 catggtgctc ggcctgcagg gttacgagat gctgaagcgg gtgggttccg aggagtacct
     3241 cggcacggcc gcggcgctca gcctgctcaa ggaactcggc cccgtcgtca ccgcgctgct
     3301 gttcgcgggc cgagccggca cggcgctcgc cgcggagatc ggcctgatgc gagccaccga
     3361 ccagctcgcg gccatggaca tcatggccgt cgacccgatg cgacgcgtgg tggcgccgcg
     3421 cttcatcggt ggcgtgatcg cgatgccgct gctgacggcg acgttcataa cgatcggcat
     3481 ctatggagcg cagctgatcg gcgtgcagtt cttcggaatc gaccagggca gcttctggtc
     3541 gcagatccgc ggcgcggtgg aactcgacga cgtcggcgaa ggtctgatca agggtgcagt
     3601 gttcggagtc gcctgcagct tgatcgccgt ttacgagggt taccacgccg tcccgacggc
     3661 cgaaggcgtc ggtcgcgcca cgacacgcac agtggtcgtg tccgccgtcg tcacgctggt
     3721 cctcgattac ctgctcacgg ccctcatgct ctgacgaccc cgaggcgaca catgcgtcca
     3781 tcgcgcacga tcgacatttc aaccggcctg ttcgtcctgc tcggcttcgc ggccatcctg
     3841 ttcctggtca cgcagatcac gaaccgcgag ttcacgctga acagcacgtc ctatcggctg
     3901 agggcagact tcgagaacgt cggaggcttg aagcctggcg cgccagtgtc catggcaggc
     3961 gtcacggtcg gccgcgtcga gaacatccag tacgaccaga aactctacaa agcggtggtc
     4021 acgctgcgcg tcgacgggcg ttacgacgag atcccgaacg acagcgacgc cagcatcctg
     4081 acctcggggc tgctcggcgg ccagtacgtc gggctgtcgc ccggcggctc cgacgagtac
     4141 ttcaaggacg gagaccaggt cacgttcgtg caggacgccg tcgtgctcga gagcctgatc
     4201 agcaagtacc tgttcaacac ggcgagcaag cagcagacgc ccgaacccgc cgcggatgcg
     4261 gcgaccgccc cgcaggagtg agtggaatgc gcatgaatgg aatcatcgga acactgaccg
     4321 tcgtcgccgt gctcggcgcg gtgccgctcg ccgtcgcttc ggcccaggcg gccgagaagc
     4381 ccgccaccgc ggccgccgtg ccgggtccgg gcccgcagca ggtgatccag aaggtgtccc
     4441 aggacctgct gcgtgacctc gacgtgaacc gggcgacata caagaaagag ccgcagaagc
     4501 tgcgcgcgct cgtcgacaag cacctgctgc cgaatttcga cgtcgactac gcggcgcgcc
     4561 tcgtgctcgg caagcactgg cgttcggcct cggaggcaca gcgcaagcgc ttcatcgacg
     4621 cgttctacca gtcgttgatg cgcaactacg gcgacgcgat cgtcgagttc caggagaacc
     4681 agctgaagat cctgccgttt cgcggtgatc ctgcgtccgg cacggcgacc gtgcgcaccg
     4741 agatcatgcg gcagggcacg ccggtgccgg tcaactacag catgcgggcg acgccgcagg
     4801 gctggaaggc gtgggacgtc acgatcgaag gcatctcgta cgtgaagaac taccgcaccg
     4861 acttcggcgc acaggcggac aaggagggca tcgacgcggt gatccagcgg ctcgagtcgc
     4921 agaatgcggc aggcaagccc gatccgaacc tgaaggcgcc ggcgaaggcc gcaaccaagt
     4981 cctgacgtcg gcaccatggc agccttcgaa atccgtgcga cgtctcccgg gcgcctcgag
     5041 gcgcaagggg cgatctgcta cgacggcgcc gcgcgggcgc tcaaggccgg gctcgacctg
     5101 atcccgcgtg ggcagccctg cacgatcgac ctgtcgcggg tcacggaagc cgacagcgcc
     5161 ggtctcgccg tgctcgtcga gtggctcgcc accgcacgca agcgccagtc ctcgatccgg
     5221 tacgagggga tcccggcgca gatcctggcc gtggcgcgga tcagcgacct ggacgacctg
     5281 ctgctcgggg aaagtggtta gtggtcagtg gccagtggtc agtggtcagt gcagaaggaa
     5341 ggccggcatt tgccgtccgt ccgcggtttc gactagccac tagccactat tcttcctctt
     5401 ccgcttcgtc ttcccgcagc gcctcttccg cccagtccga atcgtcctcg agttcttcct
     5461 ggggcacgtc gccgtcgtac acggcgtact gccgacgctg caggtaggcg tcgcgtacga
     5521 acgcgtacgg gtcgtaggct tcgttgacga ggcggtcgag ctggagcagg cccgcacgct
     5581 tgtcgaccag gtacacggcc agcgagaagt accgctcgtt gcccgcgtcg taccagcgga
     5641 acggctgggt gaaatcgtcg gcgatgcgcg ccggcgcgtc gcgcagcgtc gcagggccga
     5701 gcaacggcag catcaggtac gggccgggcg gcacgcccca gcgaccgagc gtctggcccg
     5761 agtcctcgtc gtggacgggc agcctggcgc cagtcgcgac gtcgaagatg ccaccccagc
     5821 ccagcgtggt gttgatgacg aaccgcgcga tgtcctggct gccttccttg agcttgccct
     5881 gcaggaactg gttcacgatc gtcgtcggat agaacaggtt cgtgaagaag tttccgacgc
     5941 ctttctgcag ccacgccggc gtgtggtcgc ggtaggcaac cgcggccgga cgcagcgcgt
     6001 gccggtcgat cgcatcgttg aacttgtaaa cgccgcggtt gaagccctcg atcgggtccg
     6061 ggttatgggc cgcgcgcgcc tccgccgctt cttcctcgct cgccgtgctg cccttggctg
     6121 cggacgtggt cgcgcaaccg ccgagcgcca cgaccgccag tagagatatc accagcccgc
     6181 gcacgagcgc gacgttcggg caagccaacc gcgccgggcc gcggtccaga gacgac
//
LOCUS       DASPFR010000173         5412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1027782, whole genome shotgun sequence.
ACCESSION   DASPFR010000173 DASPFR010000000
VERSION     DASPFR010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027782"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VD737_04405"
     CDS             <1..378
                     /locus_tag="VD737_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXW09833.1"
                     /translation="LTVFIEIDDEPVYTVNGRHVISEIVELCGGRNVFAELPQLAPPI
                     ATEAVLAADPQVIVTTDDTIADPAAIWRQWPRLRAVQAGTVYSLSSDLVTRASPRLAQ
                     GVEVTCRALEQAREIYRRRGGAR"
     gene            complement(392..940)
                     /locus_tag="VD737_04410"
     CDS             complement(392..940)
                     /locus_tag="VD737_04410"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HXW09834.1"
                     /translation="MGHRLSKIYTRTGDDGSTGLGDGTRVPKEHLRVEAYGTVDEANS
                     ALGVVLAVPGLPEAVLHALTEVQHDLFDLGGELCIPGHRAISAADVERLEQTLDRFND
                     DLPPLKDFILPGGGPAAAACHLARTVTRRAERRVWALARAEAVAPEVAQYLNRLSDLL
                     FVVARVLARHEHGSEVLWRRRR"
     gene            complement(955..2835)
                     /locus_tag="VD737_04415"
     CDS             complement(955..2835)
                     /locus_tag="VD737_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXW09835.1"
                     /translation="MRSVHACLARGVAKCAVVPLILAGATPASADDPDDAPATVVVTA
                     TRVPAPLQEVLAPVIVIDRATIERSAATDAADLLRFHAGLDVARNGGPGQPTSVFIRG
                     AESNHTLVLIDGVRINPGTIGLPALQNVTPDMVERIEVVKGPRSALWGSDAIGGVINV
                     VTRRGSRDGWVAEAGYGDYDTRRASLNGGIDLGERTALDFGMSWLDSAGFPTRTDDPT
                     DRGYENLNANVQLRADVGPGEVSLQHWRAAGTSEYSDFFLTPVDQDFETSTTSAAMQL
                     PVGTTGAARVSVARLEDSIDQNQSADFLHTERDSVDAQFDWQASQAQAFSVGAMYYSE
                     DASSESYGTGFEERTHAVSAYVQDRVVAGPHLALLALGYTDHQTAGSAVTWNLEYGYT
                     FNEGRTRAFGLSGAGFRAPDATDRYGYGGNPALEPERSRNYELGLRHALDSRHTVTVS
                     AFQTDVEDLIEFVVTDFETFEGINRNVAEARIRGLEASWRYAGETWQAHVEATRQEPR
                     NLADDSVLLRRAEESVTAAVTRAFGPVQLGLDVLWAGDRTDLGATGPVTLESYVLANL
                     TAQWQATRSLALVARVENLLDEDYELVSTYNTPDRGVYFTVRYTPGRPDPTVRTAATS
                     AR"
     regulatory      complement(2974..3127)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3245..4012
                     /locus_tag="VD737_04420"
     CDS             3245..4012
                     /locus_tag="VD737_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXW09836.1"
                     /translation="MPHSDFLATALEAAHAAADVVRHYYQRNLEVTIKADKSPVTEAD
                     VETEKTIRAILAAKFPGHGFHGEETGTTALDAEYVWFVDPIDGTKAFVREYPMFSTQI
                     ALARRGRLVVGVSSAPAYGELAYAEIGVGAWLNEQPIRVSAVDRIEDAALSTGNLKTL
                     ATGSRWPAFGRLVGRLNRIRGYGDFLHYHLLASGRIDAVVESDVNILDVGACAVIVEA
                     AGGRFTDLEGRPLTAASGSVLATNGRLHDAIHAAIQG"
     gene            4038..4994
                     /locus_tag="VD737_04425"
     CDS             4038..4994
                     /locus_tag="VD737_04425"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HXW09837.1"
                     /translation="MATNVRKIIAVVGATGSQGGGLVRSILDDRDGEFAVRALTRDPQ
                     SDKARALAAAGAEVVQADQDDAASMRRAFEGVHGAYCVTSFWDHMSPDRERDQARTQA
                     TAAREARVEHVIWSTLEDTRRWVPLSDPRMPTLQGRYKVPHFDVKGEADEFFAATGVP
                     TTYLLTSFYWDNYIKFGLGPKPGPDGRLVLSMPLGDRPFPSIAAEDIGRCAHAIFRRG
                     AEFANRRVGIAAGHLTGEQMASGLARALGREVSYQAVPFDVFRGLGFPGADDLGNMFQ
                     FNHDFSDEFCRSRSPEFTRALYPRLQTYDQWLAAHASEIPLG"
     gene            5017..5352
                     /locus_tag="VD737_04430"
     CDS             5017..5352
                     /locus_tag="VD737_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HXW09838.1"
                     /translation="MQSWLYLLVAIVAEVIATSALEASATFSRPGPSVVVIAGYGVAF
                     YFLARALETVPVGIAYAIWSGIGILLITVIAWLVYGQALDVPALVGLALIVAGVVVIN
                     VFSKTVVAH"
BASE COUNT          768 a         1884 c         1908 g          852 t
ORIGIN      
        1 ctcaccgtct tcatcgagat cgacgacgag ccggtctaca cggtcaacgg acgccacgtc
       61 atcagcgaaa tcgtcgaact ctgtggcggc cgcaacgtgt tcgcggagct gccgcaactc
      121 gcgccgccga ttgcgaccga ggccgtgctc gcggctgatc cgcaggtcat cgtgacgacc
      181 gacgacacga tcgcagaccc ggcggcgatc tggaggcaat ggccgcggct gcgcgccgtg
      241 caggcgggga cggtgtattc gctttcgtcg gacctcgtga cgcgggcctc gccgcggctc
      301 gcgcagggcg tcgaggtgac ctgccgcgcc ctggagcagg cgcgcgagat ctatcgccgg
      361 cgcggcggcg cccggtaggc cggcgaagcc ctcagcggcg ccggcgccag agcacctcgc
      421 tgccgtgctc gtgtcgcgcc agcacgcgcg cgacgacgaa cagcaggtcg gagagccggt
      481 tcaggtattg cgccacctcg ggcgcaacgg cctcggcacg cgcgagcgcc cagacgcgtc
      541 gttccgcgcg gcgcgtgacg gtgcgcgcga ggtgacacgc ggcggccgcc ggtccaccgc
      601 ccggcaggat gaagtccttg agtggcggca ggtcgtcgtt gaagcgatcg agcgtctgct
      661 cgaggcgttc gacgtccgcg gccgagatcg cgcggtgccc cggaatgcag agctcgccgc
      721 ccaggtcgaa caggtcgtgc tgcacctccg tcagggcgtg caggacggcc tccggcagtc
      781 ccggcaccgc cagcacgacg ccgagcgcgc tgttggcctc gtccacggtg ccgtacgcct
      841 cgacgcgcag gtgttccttc ggcacgcgcg tgccgtcgcc caggcccgtg ctgccgtcgt
      901 cgccggtgcg cgtgtagatc ttgctgaggc ggtggcccat ggctcgtgac ccggttacct
      961 ggcagatgtg gcggcggtgc ggacggtcgg gtccggacgg cccggcgtat agcgcaccgt
     1021 gaaatacact ccgcggtcgg gcgtattgta ggtgctgacc agttcgtaat cctcgtccag
     1081 caggttctcc acgcgcgcga ccagcgcgag gctgcgcgtc gcctgccact gcgccgtgag
     1141 gttggcgagc acgtagctct cgagcgtgac gggcccggtc gcgcccaggt ccgtgcggtc
     1201 gcctgcccag agcacgtcga gtccgagctg cacgggaccg aacgcgcgcg tcacggccgc
     1261 ggtcacgctt tcctcggcgc ggcgcagcag caccgagtcg tcggcgaggt tgcgtggctc
     1321 ctgccgggtg gcctcgacgt gcgcctgcca ggtctcgcca gcgtaccgcc acgaggcttc
     1381 gaggccgcgg atgcgtgctt cggcgacgtt gcggttgatg ccctcgaacg tctcgaaatc
     1441 ggtcacgacg aactcgatca ggtcctcgac gtcggtctgg aaagccgaca cggtcaccgt
     1501 gtgccgcgag tcgagcgcgt gtcgcaggcc gagctcgtag ttgcgcgagc gctcgggttc
     1561 gagggcggga ttgccgccgt agccatagcg gtccgtggcg tcgggcgcgc ggaagcccgc
     1621 gcccgacagg ccgaacgcgc gcgtacgtcc ctcgttgaag gtgtagccgt actccaggtt
     1681 ccaggtcacg gccgagccgg cggtctggtg gtccgtgtaa ccgagtgcca gcaacgccag
     1741 gtggggtccc gcgacgacgc ggtcctgcac gtaggcgctc acggcgtgcg tccgctcctc
     1801 gaagccggtg ccgtacgact cgctgctcgc gtcctccgag tagtacatcg caccgacgct
     1861 gaacgcctgg gcttgcgacg cctgccagtc gaactgcgcg tcgaccgagt cacgctccgt
     1921 gtgcaggaaa tccgcagact ggttctggtc gatgctgtct tcgagccggg cgaccgagac
     1981 gcgcgcggcg ccggtcgtcc cgaccgggag ctgcatcgcg gccgaggtcg tcgaggtctc
     2041 gaagtcctgg tccacgggcg tcaggaagaa atccgagtac tcggacgtgc ccgcggcacg
     2101 ccagtgctgc agcgagacct cgccgggtcc gacgtcggct cgcagttgca cgttggcgtt
     2161 caggttctcg tagccgcggt ccgtcgggtc gtccgtgcgc gtgggaaatc cggcgctgtc
     2221 gagccacgac atcccgaaat cgagcgccgt ccgctcgccc aggtcgatcc cgccgttcaa
     2281 gctcgcccgg cgcgtgtcgt agtcgccata accggcctca gcgacccagc cgtcgcgcga
     2341 gccgcgccgc gtcacgacgt tgatcacgcc gccgatcgcg tcgctgcccc acagcgccga
     2401 ccgcggtccc ttgacgacct cgatgcgctc gaccatgtcg ggcgtgacgt tctgcagcgc
     2461 cggcaacccg atcgtgccgg gattgatccg cacgccgtcg atcagcacga gcgtgtggtt
     2521 gctctcggcg ccgcgtatga agacggacgt gggctgcccg ggtcccccgt tcctcgcgac
     2581 gtcgagcccg gcgtggaagc gcagcaggtc cgccgcgtcg gtggcggcgc tgcgctcgat
     2641 cgtcgcgcgg tcgatcacga tcacgggcgc gagcacttcc tgcagcggcg ccggcacgcg
     2701 cgtcgcggtg accacgacgg tggctggtgc gtcgtccggg tcgtcagccg acgcgggcgt
     2761 cgcgcctgcg aggatcagcg ggacgacggc gcacttcgcg acgccacggg caaggcaggc
     2821 atgaacggaa cgcatgttgg ctacaccccg agcgagcggc gtcgaaacgc cggtgacaat
     2881 ccagctgcgg ggtgatggcg gaaatctcgt acgggttctg cagcccggat ccgcgccgct
     2941 cgcccaccgc gaggctggtc tcgtccgacg ggccggtctc cgggctctcg agcgggcggt
     3001 gggccctgcc tgcatcccct tcccgcgcct gcggcgcagt gggttccgga ctcgctcgtc
     3061 cggacgatgc aggcttcact cgactaccgt tgcgggggca gcgccggaat tggaccggct
     3121 tcccgttctc cctgagccct ggctcgcggg atcacctgtc ggtgcgcgca cggtaggcaa
     3181 gcgggtgcgg taagtcaagg aaatgcgggt accatggcgg cggtcgacgc caccgactgc
     3241 ctgcatgccg cactccgatt tcctcgcgac tgcactcgag gccgcgcacg ccgcggccga
     3301 cgtcgtgcgg cactactacc agcgcaacct cgaggtcacg atcaaggcgg acaagtcgcc
     3361 ggtgaccgag gccgacgtcg agaccgagaa gacgatccgc gcgatcctcg ccgcgaaatt
     3421 cccagggcac gggttccacg gcgaggagac cggcacgacg gcgctcgacg ccgagtacgt
     3481 ctggttcgtc gacccgatcg acggcaccaa ggcgttcgtg cgcgagtatc cgatgttctc
     3541 gacgcagatc gcgctcgcgc gtcgcgggcg cctggtcgtc ggtgtctcct ccgcaccggc
     3601 ctacggcgaa ctcgcgtatg cggagatcgg cgtgggcgcc tggctcaacg agcaaccgat
     3661 ccgcgtgagc gccgtcgacc ggatcgagga cgccgcgttg tccaccggca acctgaagac
     3721 gctggcgacc gggtcccgct ggcccgcgtt cggtcggctg gtcggccgcc tgaatcgcat
     3781 ccgcggctac ggcgacttcc tgcactacca cctgctggcc tcgggtcgca tcgacgcggt
     3841 cgtcgagtcg gacgtcaaca tcctcgacgt gggcgcctgc gccgtgatcg tggaggccgc
     3901 cggtggacgc ttcaccgacc tcgagggccg gccgctgacc gcggcgagcg gcagcgtgct
     3961 cgcgaccaac ggccgactgc acgacgccat acacgcagcg atccaaggct gagcctgcgc
     4021 tgcaacggag gttcaacatg gcaaccaacg tcaggaagat catcgcggtc gtcggcgcca
     4081 ccggttcgca gggcgggggg ctcgtgcgct cgatcctcga cgatcgggac ggtgagttcg
     4141 cggtccgcgc gctcacccga gatccgcagt cggacaaggc gcgggcgctg gccgcagccg
     4201 gggccgaggt cgtgcaggcc gaccaggacg atgctgcgtc gatgcgccgt gcgttcgaag
     4261 gcgtccacgg tgcctattgc gtgacctcgt tctgggatca catgtcgcca gaccgcgagc
     4321 gcgatcaggc acggactcag gcgacggctg cgcgcgaggc acgggtcgag cacgtgatct
     4381 ggtcgacgct cgaggacacg cgacgctggg tgccgctgtc ggatccgcgc atgccgacgc
     4441 tgcagggtcg ctacaaggtg ccgcacttcg acgtcaaggg cgaggccgac gagttcttcg
     4501 ccgcgacggg cgtgcccacg acctacctgc tcacgtcgtt ctactgggac aactacatca
     4561 agttcgggct ggggcctaag ccggggcccg acggccggct cgtgctgtcg atgccgctcg
     4621 gcgatcgacc gttcccgagc atcgctgcgg aagacatcgg gcgctgcgcc cacgccatct
     4681 tccgccgcgg cgccgagttc gcgaaccggc gggtcggcat cgcggccggg catctcaccg
     4741 gcgagcagat ggcgagcggg ctggcgcggg cgctcggtcg cgaggtctct taccaggccg
     4801 tgcccttcga cgtgttccgc ggcctgggat ttcccggcgc cgacgacctc ggcaacatgt
     4861 tccagttcaa ccacgacttc agcgacgagt tctgccgctc gcgcagcccg gagttcacgc
     4921 gggcgctgta cccgcggctg cagacgtacg accagtggct cgcggcgcac gcaagcgaga
     4981 tcccactcgg ctgaccggcc gccgccgcgc atgtcgctgc aatcgtggct gtacctgctc
     5041 gtcgcgatcg tcgccgaggt gatcgccacc tcggcgttgg aggcgtccgc cacgttcagc
     5101 cggcccgggc cttcggtcgt ggtgatcgcg ggctacggcg tggccttcta cttcctggca
     5161 cgcgcgctcg agaccgtgcc ggtgggcatc gcctacgcga tctggtcggg catcggcatc
     5221 ctgctgatca cggtgatcgc ctggctggtc tacggccagg cgctcgacgt gccggcgctc
     5281 gtcgggctcg cgctgatcgt cgcgggtgtc gtcgtgatca acgtattctc gaagaccgtg
     5341 gtcgcgcact gaatccgcac gctagatccg cgcactagtc cgcgtcacgg ccgatccaca
     5401 aggcgattcg cc
//
LOCUS       DASPFR010000174         3966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_539279, whole genome shotgun sequence.
ACCESSION   DASPFR010000174 DASPFR010000000
VERSION     DASPFR010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539279"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VD737_04435"
     CDS             complement(<1..314)
                     /locus_tag="VD737_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09839.1"
                     /translation="MTDASVSPMGSDPGFAPPRRFRHHHLQSIYPSLPIRRRTVERRC
                     APLLGAARTEILDCGDGVRLLAQVTTQVRLGRAPSDKLAVLLHGWEGSAQSLYLLSLG
                     QT"
     gene            complement(365..1834)
                     /locus_tag="VD737_04440"
     CDS             complement(365..1834)
                     /locus_tag="VD737_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXW09840.1"
                     /translation="MTTGKRALIVDDSRSARVILSRMLEQHGMTVDTAESAEQALEYL
                     QRTRPDVIFMDHLMPGMDGFQAVQAIKADPLTATIPLMMYTSQEGELYVSQARALGAV
                     GVLPKTVRPVDVSRILYQLHLLPDRRTQRSALFPGPAAAAAQAVESGNGHEAPIAAAL
                     AAGQADEAAATGTRPAMAPLGMPTAAETTPALAFTPAMVNDLQAGLRQSTQQLVKDQL
                     GEQRRFMLATFEAFARRMSTELREGLAKIPPPPSPESLIPPSPPRQWWPPVMTALLAV
                     IPAVVLGYMMWQTSMTNERLARELVETRAVAERAKADAIAATARASATVASTEPMSGT
                     PGEEEAVATEYVPYGEAPFSGGRLERLRALASLLEEKQFKGSIRVESFVGDFCLAGTT
                     GEGFAIAPGDTPVAKCDLVGNPYDEALSPAQRQSLDFANYAAMLRKRTGGAIRVEVVN
                     AGRREPIAYPEQGDKVTASAWNVVAAQNNRVEFHLVPAA"
     gene            1999..>3966
                     /gene="fusA"
                     /locus_tag="VD737_04445"
     CDS             1999..>3966
                     /gene="fusA"
                     /locus_tag="VD737_04445"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXW09841.1"
                     /translation="MPYTTRDIRNLALVGQAGAGKTLLAEALLAQSGAIRSKGSLARG
                     TTVCDFDPQEKALLHSLDAAICGFETQGKRVNVIDTPGYPDFMGRALSALEAVETAVI
                     VVSAVNGVETMTQRMMDFAKQRGLCRLIVVNKIDSRDARAEEVLEEIRENFGRACLPL
                     NLPAERGSAVVDCFFQPDGKPTDFSSCKDAHTQIIDQVVEVDEELMALYLEQGEELNP
                     DQLHDPFEKALREDHLVPICFCSAETGAGIPELLDVLARLAPNPAEGNPPPFLKGEGE
                     SAERVEVSPDPAKHVIAHVFKVTIDPFVGKLGVFRVHQGTVKPGAQLYVGDARKPVKL
                     AHIYQLQGKDHVEVQQAVPGDIVAVPKIDEIFFDAVLHDSHDEDHYHLKSVTFPPAML
                     GLAIRSEKRGDEQKLSEGLHRLTAEDPCVRVEHHVAQNETVLYGLGDLHLRVMLQRLS
                     DRYGVSVKTSPPSVPYRETITRPAEGHCRHKKQTGGAGQFGEVYLRIEPLERGAGFEF
                     VDDVVGGAIPGQFIPAVEKGVRQVLTEGAVAGFELQDVRVSVYDGKHHPVDSKEVAFV
                     SAGRKAFIEAIRSAGPIVLEPIVRVAISTPSGAIGDITSDLATRRARINGQDSLAGGR
                     AEITALVPLAELQDYLSRLKALTGGEGTYTMD"
BASE COUNT          636 a         1368 c         1370 g          592 t
ORIGIN      
        1 aacgtctgcc cgagcgacag caggtacagc gactgtgcgc tgccttccca accgtgcagc
       61 agcaccgcca gcttgtcgct cggggcacga cccaggcgca cctgcgtggt gacctgggcg
      121 agcaaccgca cgccgtcgcc acagtcgagg atctcggtcc gtgccgcgcc gagcagcggc
      181 gcgcaccggc gctcgaccgt gcggcgccgg atcggcaggc tcggatagat cgactgtagg
      241 tggtgatggc ggaaccggcg gggcggcgcg aatccagggt ccgaacccat cggcgaaacg
      301 ctcgcgtcgg tcatgagcgc aggacgcggt cccccgctcg cgcgtcagtc atgggagttc
      361 acactcaggc ggcggggacc aggtggaatt cgacgcgatt gttctgcgcg gccacgacgt
      421 tccaggcgct cgcggtcacc ttgtcgccct gttccgggta ggcgatcggc tcgcgccgac
      481 ccgcgttgac gacctcgacg cggatcgccc cgccggtgcg cttgcgcagc atcgccgcgt
      541 agttcgcgaa atcgagcgac tgccgctgcg ccggcgacaa agcctcgtcg tacgggttgc
      601 cgacgaggtc gcatttcgcg accggcgtgt cccccggcgc gatcgcgaag ccctcgcccg
      661 tggtgccggc caggcagaag tccccgacga acgattccac gcggatgctg cccttgaact
      721 gcttctcctc gagcagcgac gccaatgcgc gcaggcgctc gagccgcccg cccgagaacg
      781 gcgcctcccc gtagggtacg tactcagtgg ccaccgcctc ttcttcgccc ggcgtgccgc
      841 tcatgggctc cgtgctcgcg accgtggccg aggcgcgcgc cgtggcggcg atcgcgtcgg
      901 ccttcgcgcg ctcggcgacc gcccgtgtct cgacgagctc gcgcgcgagc cgctcgttgg
      961 tcatcgaggt ctgccacatc atgtagccga gcacgactgc cgggatcacc gcgagcagcg
     1021 cggtcatcac ggggggccac cattgccgcg gcggcgacgg cggaatcagc gactccggcg
     1081 acggcggcgg cggaattttc gcgaggcctt cgcgcagctc cgtgctcatg cggcgcgcga
     1141 acgcctcgaa cgtcgcgagc atgaagcgtc gctgctcgcc gagctggtcc ttgacgagct
     1201 gctgggtcga ttggcgcagc cccgcctgca ggtcgttgac catggcaggg gtgaacgcga
     1261 gtgcaggtgt cgtctccgcg gcggtcggca tgcccagcgg tgccatggcc gggcgcgtgc
     1321 cggtcgcggc ggcctcgtcg gcctgccccg cggccagggc ggccgcgatc ggcgcttcgt
     1381 gtccgttgcc gctttcgaca gcctgcgccg ccgcggcggc gggtcccgga aacagtgcgc
     1441 tgcgctgcgt ccgacgatcg ggcagcaggt gcagctggta gaggatccgc gacacgtcga
     1501 cggggcgaac cgtcttcggc agcacgccca ccgcgccgag cgcgcgcgcc tggctcacgt
     1561 agagctcacc ctcctgcgac gtgtacatca tcagcgggat cgtggccgtc aaggggtcgg
     1621 ccttgatggc ctgcacggcc tggaatccgt ccatgccggg catcaggtgg tccatgaaga
     1681 tcacgtccgg acgcgtccgt tgcaggtact cgagcgcctg ctcggccgac tctgccgtgt
     1741 ccaccgtcat cccgtgctgc tcgagcatcc ggctcaggat cacccgggcc gaacgcgagt
     1801 cgtcgacgat cagggcgcgc ttgcccgtgg tcattcgccg gatccctttt ggagacgccg
     1861 cgacgggcgc cattgtgtac cggctttcta ccttgaaaga gcgtcgaagc caatcccggg
     1921 tattggaagt cgccccgtgg ggccccgggg ttatgcttcg gtcattgcat cgacaagacc
     1981 cgaaacaagg agccctgcat gccctacacc actcgcgaca tcaggaacct cgccctcgtg
     2041 ggccaggcag gcgcgggcaa gacgctgctg gccgaagcct tgctcgcgca gtccggagcg
     2101 atacgctcaa agggttcgct cgcccgcggg acgacggtct gtgatttcga tccgcaggag
     2161 aaagccctgc tgcattcgct ggacgcggcg atctgcggct tcgagaccca gggcaagcgc
     2221 gtcaacgtga tcgacacgcc cggatacccg gacttcatgg ggcgcgcgct gagcgcgctc
     2281 gaggcggtcg agacggccgt gatcgtcgtg agcgccgtca acggcgtcga gacgatgacc
     2341 cagcgcatga tggacttcgc aaagcagcgc ggcctctgcc ggctgatcgt ggtcaacaag
     2401 atcgacagcc gcgacgcgcg cgccgaggaa gtgctcgagg agatccgcga gaacttcggg
     2461 cgcgcgtgcc tgccgctcaa cctgcccgcg gagcgcggct cggccgtcgt cgactgcttc
     2521 ttccagccgg acggcaagcc gaccgatttc tcctcgtgca aggacgccca cacgcagatc
     2581 atcgaccagg tcgtcgaggt ggacgaggaa ttgatggccc tctacctgga acagggagag
     2641 gagctgaacc ccgaccagct ccacgacccg ttcgaaaagg cgctgcgcga ggaccacctc
     2701 gtgccgatct gcttctgctc cgccgagacc ggagcgggca tccccgaact gctcgacgtg
     2761 ctcgcgcgcc tggcgccgaa tccggccgag ggcaacccgc cgccgttcct caagggcgaa
     2821 ggcgaatccg ccgagcgggt cgaggtgagc cccgacccgg ccaagcacgt gatcgcccac
     2881 gtgttcaagg tcacgatcga cccgttcgtc ggcaagctgg gcgtgttccg cgtgcaccag
     2941 ggcacggtga agccaggcgc acagctctac gtcggtgacg cccgcaagcc ggtgaagctg
     3001 gcgcacatct accagctgca gggcaaggat cacgtcgagg tgcagcaggc cgtgccgggc
     3061 gacatcgttg cggtgccgaa gatcgacgag atcttcttcg acgccgtgct gcacgactcg
     3121 cacgacgagg accactatca cctcaagtca gtgaccttcc cgcccgcgat gctcggcctc
     3181 gcgatccgca gcgagaagcg cggcgacgag cagaagctgt cggagggact gcaccgcctg
     3241 acggccgagg acccgtgcgt acgcgtcgag caccacgtcg cgcagaacga gacggtgctg
     3301 tacgggctgg gtgacctgca cctgcgcgtg atgctgcagc ggctctcgga ccgttacggc
     3361 gtctcggtca agacgagccc gccgagcgtg ccgtaccgcg agaccatcac gcgaccggcc
     3421 gagggccact gccggcacaa gaagcagacc ggcggcgccg gccagttcgg cgaggtgtac
     3481 ctgcgcatcg agccgctcga gcgcggcgcg ggcttcgagt tcgtcgacga cgtggtcggg
     3541 ggcgcgatcc cgggacagtt catcccggcc gtcgagaagg gcgtacggca ggtgctgacc
     3601 gagggcgcag ttgccggctt cgagctgcag gacgtgcgcg tgagcgtcta cgacggcaag
     3661 caccacccag tcgattccaa ggaagtcgcg ttcgtctcgg cgggccgcaa ggcattcatc
     3721 gaggcgatcc gcagcgccgg cccgatcgtg ctcgagccca tcgtgcgcgt ggcaatcagc
     3781 acgccgtccg gcgcgatcgg cgacatcacc agcgacctcg cgacgcgtcg cgcgcgaatc
     3841 aacggccagg attcgctggc cggcggacgc gccgagatca cggcgctggt gccgctcgcg
     3901 gagctgcagg actacctgtc gaggctcaag gcgctgacgg gcggcgaagg cacgtacacg
     3961 atggac
//
LOCUS       DASPFR010000175         4687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1150482, whole genome shotgun sequence.
ACCESSION   DASPFR010000175 DASPFR010000000
VERSION     DASPFR010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150482"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VD737_04450"
     CDS             <1..999
                     /locus_tag="VD737_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXW09842.1"
                     /translation="VASGTVRTAPLPAGIVGGIEIAPWGEIGLTFTSARSPADAFSVD
                     PMTLAVSRWTTSETGGLDPNNNVEPETMEVRSFDGLVISGFLYRPDRTRFPGPRPLIV
                     NIHGGPESQSRPGFLGRNNYLINELGIAIFFPNVRGSSGYGKRFVSLDNGPLRREDSV
                     KDIGAFLDALARDPLLDANRFAVTGGSYGGYMCYATSIFYGARLEGANCVVAISSFVT
                     FLENTQSYRRDLRRVEYGDERDPRQRRKLLEISPLTRVQEIRLPMLVVTGANDPRVPA
                     SEADQIVAAIRANGGSAWHLLGQDEGHGFAKKANQDYQFWTSLMFWEQTLLAPKRE"
     gene            1128..2141
                     /locus_tag="VD737_04455"
     CDS             1128..2141
                     /locus_tag="VD737_04455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HXW09843.1"
                     /translation="MLQLGYKLMTETTDAKALVRNARMAEDAGFDFVAISDHFHPWLE
                     SHGHSPFAWSVLGAIATSTSRVGIATGLTCPTMRYHPAIVAQAAATVGVMCDGRFTLA
                     VGAGERLNEHVVGLGWPAVHERHDMLREAIEIIRLLWRGETCSHQGEYYHLDSARLYD
                     LPEKPIPLVVGVSGDKSVQLAAEVADGIMAVEAKRELVDDWRKAGGGDGPRYGEMSLG
                     YARSKRAGLELVHRYMKFGAFDWSVLAELPNVGSFEAASKYVRPKDLEDEIPHGPGVE
                     PYVESVRKFVDAGFDRVALLAVGEDQETFMRFFERELADALRALEPRSAKASTKKTIK
                     RRS"
     gene            2439..4655
                     /locus_tag="VD737_04460"
     CDS             2439..4655
                     /locus_tag="VD737_04460"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HXW09844.1"
                     /translation="MIRQILIRYAVPIAAAVSLVAFVGVPVIDSILAGWFRADVRTRA
                     RLVMTSIEDTLRTVLETGDPSRAIAYLDRISGDEKMLGVVVCARDDGEVVRTERVPTI
                     VSCASTAPVEAADALRSSLEETLDVSEFPIETVAGERYRVLVVHDLGFIDRRQTTARN
                     FILAFTVLAAGALAFVVAFAIRALLQRWIRLLVGDISSRRFLDDAESSGASLPVLMQV
                     RQALRELEATQRLEIGFQENWTPQALQQVMREQLNDCPMLVVSNREPYIHNLDASGRP
                     VVQVPASGMVTAIEPIMRACSGTWIAHGSGSADRLVVDRHDHVRVPPDDPTYDLRRVW
                     LTPEEEEGYYYGFSNEGLWPLCHLAYVRPAFRERDWQAYRTVNRRFAEVVAREAKGVE
                     PVVLIQDFHFALLPRLIREQGVNATIAMFWHIPWPNAETFGVCPWKREILEHMLGADI
                     LGFHTRYHCHNFLDTVDRYIECQIDQEHMTITHRGHVCRVKPYPISIEWPPRWLAGVA
                     PAAEARAAVSARFDLPPSVRIGVGVERWDFTKGIIERFLALEVLFEKHPELRGRLTFL
                     QVAAPSRSKLPAYQALQEQTLRELNRVNLRFGTGSWQPIVLIGEHQEPAQVFELYRAA
                     DFCLVNSLHDGMNLVAKEFVASREDEDGVLILSNFTGASRELVEALLVNPYDVSETAD
                     AMARALTMPRNERRQRMHLMRQTLSEANVYRWAGRMLIDVARIRQRQALKSTSRVA"
BASE COUNT          759 a         1626 c         1561 g          741 t
ORIGIN      
        1 gtggcgagcg gcaccgtgcg cacggcaccg ctgcccgccg ggatcgtcgg tggcatcgag
       61 atcgcgccgt ggggcgagat cggcctcacg ttcacgtcgg cgcgcagccc cgcggacgcg
      121 ttctcggtcg accccatgac gctcgcggtc tcgcgctgga cgacgagcga gaccggcggc
      181 ctcgacccga acaacaacgt cgagccggag accatggaag tccggagctt cgacggtctc
      241 gtcatttcgg gcttcctgta ccggcccgac cgcacgcgct tcccgggccc gcgccctctg
      301 atcgtcaaca tccacggcgg cccggagagc cagtcgcggc ccgggttcct cggccgcaac
      361 aactacctga tcaacgagct cggcatcgcg atcttcttcc ccaacgtgcg cgggtcgtcg
      421 ggctacggca agcggttcgt gagcctcgac aacggcccgc tccggcgcga ggactcggtc
      481 aaggacatcg gcgccttcct cgacgcgctc gccagggatc cgctgctcga cgcgaaccgg
      541 ttcgcggtga ccggaggctc gtacggcggc tacatgtgct acgcgacgtc gatcttctac
      601 ggcgcgcgac tcgagggggc gaactgcgtg gtcgcgatct cgagcttcgt cacgttcctc
      661 gagaacaccc agagttaccg tcgcgacctg cgacgtgtcg agtacggcga cgagcgcgac
      721 cccaggcagc gccggaagct gctcgagatc tcgccgctca cccgcgtgca ggagatccgc
      781 ctgccgatgc tggtggtcac cggtgcgaac gatccacgcg tcccggcgag cgaggccgat
      841 cagatcgtcg ccgcgatccg cgccaacggc ggttccgcgt ggcacctgct gggccaggac
      901 gaaggtcacg gcttcgccaa gaaggccaac caggattacc agttctggac cagcctgatg
      961 ttctgggaac agacgctgct cgcgccgaag cgcgagtgac gagccgtcga atgcacgcgg
     1021 ccatgtcgtg tgagccccct ccgacgggac gcgaacgcgc gtcctgacgc cgcggagccg
     1081 ccacacgcgc gaagatctcc gcctcggcga cgtcggcgga ggtttccatg ctgcagctcg
     1141 gttacaagct catgacggag accacggacg ccaaggcgct cgtgcgcaac gcacggatgg
     1201 ccgaggacgc cggtttcgac ttcgtggcga tctccgacca cttccaccct tggctcgagt
     1261 cccacggtca ttcgccgttt gcctggagcg tgctcggcgc gatcgcgacg tcgacgtcgc
     1321 gggtcggcat cgcgaccggc ctcacctgcc cgacgatgcg ctaccacccc gcgatcgtgg
     1381 cacaggccgc cgcgacggtc ggcgtgatgt gcgacggacg cttcacgctc gcggtgggcg
     1441 ccggcgaacg cctcaacgag cacgtggtcg gcctgggctg gccggccgtg cacgagcgcc
     1501 acgacatgct gcgcgaggcg atcgagatca tccggctgct gtggcgcggc gagacctgct
     1561 cgcaccaggg tgagtactac cacctcgaca gcgcgcgcct ctacgacctg ccggagaaac
     1621 cgatcccgct cgtggtcggc gtcagcggcg acaagtcggt gcagctcgcg gcggaagtcg
     1681 ccgacggcat catggcggtc gaggccaagc gcgagctcgt cgacgactgg cgcaaggccg
     1741 gcggtggcga cggcccgcgc tacggcgaga tgtcgctcgg gtatgcgcgc agcaagcggg
     1801 ccggcctcga gctcgtgcac cgctatatga agttcggggc gttcgactgg agcgtgctcg
     1861 cggagctgcc gaacgtcggc tcgttcgaag ccgcgagcaa gtacgtgcgg cccaaagacc
     1921 tcgaggacga gattccgcac gggccgggcg tcgaaccgta cgtcgagtcg gtgcgcaagt
     1981 tcgtcgatgc agggttcgac cgcgtcgcac tattggccgt cggcgaggac caggagacct
     2041 tcatgcgctt cttcgagcgc gagctggccg acgcgctgcg ggcgctcgag ccgcgcagcg
     2101 ccaaggcctc tacgaagaaa acgatcaagc gccggagtta gtcacgcgca cactctgcca
     2161 ctcacgcgtc caggcccgcg tgagtgacga cccgcgtgtc aacacgaaga agtccatgcg
     2221 gccgtggcgg gcttgtcgcc gtcgtcgtcg agccgcaccg cgcgcgatgt cggcgagcgt
     2281 gtcgttgcgg cctgagcgac gagtcaagcg tgagcttttc tcatcgaccg gcggtcaaat
     2341 cgtcgctacg cacggctgcg ctgtattccg tagactgttg agtcgacctc cgtcgccgca
     2401 ggcgtctaac ccccgtcggc gcaccggaca ggtaaaccgt gatccgtcag attctcattc
     2461 gctacgccgt accgatcgcc gccgcagtca gcctggttgc cttcgtcggc gtgccggtca
     2521 tcgactcgat cctcgccgga tggttccgcg ccgacgtccg cacgcgggcc cggctcgtga
     2581 tgacgtcgat cgaggacacg ctgcgaaccg tgctcgaaac cggtgacccc agtcgcgcga
     2641 tcgcctacct cgaccgcatc tccggcgacg agaagatgct cggcgtggtg gtgtgtgccc
     2701 gcgacgacgg cgaggtggtc aggaccgagc gcgtcccgac gatcgtctcg tgtgcgtcga
     2761 cggccccggt cgaagcggcc gacgcgctgc gctcgtcgct cgaggaaacg ctggacgtct
     2821 cggaattccc gatcgagacc gtcgcgggcg agcgctatcg cgtgctggtg gtgcacgacc
     2881 tgggattcat cgaccggcgc cagacgacgg cgaggaactt catcctcgcc ttcaccgtgc
     2941 tcgcggccgg cgcgctcgcc ttcgtcgtcg ctttcgcgat ccgcgcgctg ctgcagcgct
     3001 ggatccgcct gctcgtcggc gacatcagca gtcggcgctt cctcgacgac gcggagtcgt
     3061 ccggcgcttc gctgcccgtg ctgatgcagg tgaggcaggc cctgcgcgaa ctcgaggcga
     3121 cccagcgcct cgagatcggc ttccaggaga actggacacc gcaggcactg cagcaggtga
     3181 tgcgcgagca gctgaacgac tgcccgatgc tcgtggtgtc gaaccgcgaa ccctacatcc
     3241 acaacctcga cgcgtcgggt cgccccgtgg tgcaggttcc ggcgagcggg atggtcacgg
     3301 cgatcgagcc gatcatgcgc gcctgttcgg gcacctggat cgcccacggc agcggctcgg
     3361 ctgaccgcct ggtcgtcgac cgtcacgacc acgtacgggt cccgcccgac gacccgacct
     3421 atgacctgcg acgcgtctgg ctgacgcccg aggaagagga aggctactac tacggcttct
     3481 ccaacgaggg tctgtggcca ctgtgccacc tggcgtacgt acgtcctgcg tttcgcgagc
     3541 gcgactggca ggcctatcgc acggtgaacc gtcgcttcgc ggaagtcgtc gcgcgggaag
     3601 cgaaaggcgt cgaaccggtg gtcctgatcc aggatttcca cttcgcgctg ctgccgcgcc
     3661 tgatccgcga acagggcgtc aatgccacga tcgccatgtt ctggcacatc ccttggccca
     3721 acgcagagac ctttggcgtg tgcccgtgga agcgcgagat cctcgagcac atgctgggtg
     3781 cggacatcct cgggttccac acgcgctatc actgccacaa cttcctcgac accgtggatc
     3841 gctacatcga gtgccagatc gaccaggagc acatgacgat cacgcaccgc ggccacgtct
     3901 gccgggtcaa gccctatccc atctcgatcg aatggccgcc acggtggctg gccggggtgg
     3961 ccccggccgc cgaggctcgc gctgccgtgt cggcacgctt cgacctgccg cccagcgtgc
     4021 ggatcggtgt cggcgtcgag cgctgggact tcaccaaggg catcatcgag cgattcctcg
     4081 cgctcgaggt gctgttcgag aagcacccgg agctgcgcgg acgcctcaca ttcctgcagg
     4141 tggcggcgcc gtcacgcagc aagctgccgg cgtaccaggc actacaggag cagacgctgc
     4201 gcgagctcaa ccgggtcaac ctgcggttcg gcaccggcag ctggcaaccc atcgtgctga
     4261 tcggcgagca ccaggagccc gcacaggtct tcgagctcta tcgagccgcg gacttctgcc
     4321 tggtcaacag cctgcacgac gggatgaacc tcgtggcaaa ggagttcgtc gcctcacgcg
     4381 aggacgagga cggcgtgctc atcctgagca acttcaccgg cgcgtcgcgc gaactggtcg
     4441 aagcgctgct cgtcaaccct tacgacgtct cggagacggc ggatgccatg gcccgcgcgc
     4501 tcacgatgcc ccgcaacgag cggcgccagc gcatgcacct gatgcgacag accctgtccg
     4561 aggccaacgt ctaccgctgg gccgggcgga tgctgatcga cgtcgcgcgg atccggcagc
     4621 gccaggcgct gaagtccacc tcccgggtgg cgtgaccgat cgtcgcgaca gcgtcgacca
     4681 cggcgct
//
LOCUS       DASPFR010000176         5475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1760641, whole genome shotgun sequence.
ACCESSION   DASPFR010000176 DASPFR010000000
VERSION     DASPFR010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1760641"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VD737_04465"
     CDS             <1..340
                     /locus_tag="VD737_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09845.1"
                     /translation="WITLQFRYLAFAGPGRLLLAGCRGVRREPASGGRSIDQAATIGF
                     SGELDYVPRRSATFGAYLLGINGLFDDAFAGGPGVYVYEEMPYYGKRSGLTGRGLEGV
                     TDVVLQAFGI"
     gene            complement(362..1246)
                     /gene="ttcA"
                     /locus_tag="VD737_04470"
     CDS             complement(362..1246)
                     /gene="ttcA"
                     /locus_tag="VD737_04470"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HXW09846.1"
                     /translation="MSAVVIEPAAAPAAIPRRASTTFKRLQARLRGLVGKAIDDFRMI
                     EDGDRVMVCLSGGKDSYTLLDVLLSLQRSAPVRFELVAVNLDQKQPGFPAHVLPEYLT
                     ALGVPFHVIEQDTYRVVKRVVGEGRTMCGLCSRLRRGALYRYASEHGVTKLALGHHRD
                     DIVETLFLNMFFGGRLKAMPPKLLSEDGRHIVIRPLAYVPEPEIARYARARQFPIIPC
                     NLCGSQDHLQRVAVKQMLADWERAHPGRTEAIFSALGNVSVSHLADARAFDFAGLESR
                     RVAAPGPPGDREPWDDPG"
     gene            complement(1243..2838)
                     /locus_tag="VD737_04475"
     CDS             complement(1243..2838)
                     /locus_tag="VD737_04475"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HXW09847.1"
                     /translation="MAQSPYQQLEQEFRRLHAFRGAASLLRWDAAVMMPRGSADVRGE
                     QLAALDSECHALLTSPKVSRLIERAQANQAQLEDWQLANLREMRRERDRAIATPLTLV
                     TRLARATALAEVRWLEARQENEFPLFAPHLEEVVALTRDKAQVLSKALGLAPYDALID
                     GFSPGFLSADIDQLFRVVNRRLPSLIRDAIDLQSTRPPLPLAGKVSVSKQRALVLEVM
                     RALGFPFHRGRLDEASHAFTEGVWGDVRVTTRFDVQEPFTALMGVLHETGQAMYDLGL
                     PAAWRDQPVGRDRGMALEESQSLLLEMIVGRSRPFLTWLKPLLEKHFGCTGPEWSVEN
                     LYSTLTRVRRSAVRADADELTYPVHITVRYELEKEIFDGTLPVRDLPEAWRSAFERRL
                     GLRPASDAEGCLQDVHWAIGSFGYFPSYALGSIIAAQLYESLRAELEHFDADIAAGRF
                     GALFDWLRRNVHARGASVGALELIQGATGRPLSAAPWLRYAESKYLEDEGEPAAEEVA
                     TTGSAGASQPTPLAAAAPGPAES"
     gene            complement(3005..5419)
                     /locus_tag="VD737_04480"
                     /pseudo
     CDS             complement(3005..5419)
                     /locus_tag="VD737_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
BASE COUNT          868 a         1928 c         1891 g          788 t
ORIGIN      
        1 ctggatcacg ctgcagtttc gctacctcgc gttcgcaggg ccagggcgat tgctgctcgc
       61 gggctgccgc ggcgtgcgtc gcgagccggc gagcggcggt cgcagcatcg accaggcggc
      121 gacgatcggc ttcagcggcg agctcgacta cgtgccgcga cgctcggcga ccttcggcgc
      181 gtacctgctc ggcatcaacg gcctgttcga cgacgccttc gccggcggcc cgggcgtcta
      241 cgtctacgag gaaatgccct attacggcaa gcgctcgggc ctcaccgggc gcggactgga
      301 gggcgtgacc gacgtcgtcc tgcaggcgtt cggcatctga ggatgccgga ggtgagtgcc
      361 gtcagcccgg gtcgtcccag ggctcgcggt cgcccggcgg acccggcgcc gccacgcgcc
      421 gcgactcgag tccggcgaag tcgaaagctc tcgcatcggc gagatgcgag accgagacgt
      481 tgccgagcgc ggagaagatc gcctcggtgc gtccgggatg cgcccgctcc cagtcggcca
      541 gcatctgctt gaccgccacc cgctgcaggt ggtcctgcga cccgcacagg ttgcacggga
      601 tgatcgggaa ctggcgcgcc cgcgcgtacc gcgcgatctc aggctccggg acgtaggcga
      661 gcggccggat cacgatgtgg cggccgtcct ccgacaggag cttgggcggc atggccttca
      721 gccggccgcc gaagaacatg ttcaggaaca gcgtctcgac gatgtcgtcg cgatgatgac
      781 cgagcgccag cttggtgacg ccatgctcgc tcgcgtagcg gtagagcgcg ccacggcgca
      841 ggcgcgagca gagcccgcac atcgtgcggc cttcgccgac cacgcgcttg accacgcgat
      901 aggtgtcctg ctcgatcacg tggaacggca cgccgagcgc ggtcaggtac tcgggcagca
      961 cgtgcgccgg gaaacccggc tgcttctggt cgagattgac ggcgacgagc tcgaagcgca
     1021 ccggtgcact gcgttgcagc gacagcagca cgtcgagcag cgtgtacgag tccttgccgc
     1081 cggacaggca gaccatcacg cggtcgccgt cctcgatcat gcggaagtcg tcgatcgcct
     1141 tgccgaccag cccgcgcagc cgtgcctgca gtcgcttgaa cgtcgtcgag gcgcgtcgcg
     1201 ggatggcggc cggcgccgcc gccggttcga tcacgaccgc gctcacgact ctgcgggtcc
     1261 cggcgcggcc gccgcgagcg gcgtcggctg cgaggcgccg gcgctgccgg tcgtcgcgac
     1321 ctcctccgcc gcaggctcgc cctcgtcttc caggtacttg gactccgcgt aacgcagcca
     1381 gggcgccgca ctcagcggcc ggcccgtcgc gccctggatc agctcgagcg cgccgacact
     1441 ggcgccgcgc gcgtgcacgt tgcgacgcag ccagtcgaac agcgcgccga agcgaccggc
     1501 cgcgatgtcg gcgtcgaagt gctcgagctc ggcacgcagg ctttcgtaca gctgcgcggc
     1561 gatgatcgag ccgagcgcat acgacggaaa ataaccgaac gagccgatcg cccagtgcac
     1621 gtcctgcagg cagccctcgg cgtcgctcgc cggtcgcagg ccgagccggc gttcgaacgc
     1681 ggagcgccac gcctccggca ggtcgcgcac cggcagcgtg ccgtcgaaga tctccttctc
     1741 gagttcgtag cgcaccgtga tgtgcacggg gtacgtgagc tcgtccgcgt cggcgcgcac
     1801 ggcgctgcgc cgcacccgcg tgagcgtgga gtacaggttc tcgacggacc actccgggcc
     1861 ggtgcagccg aagtgcttct cgagcagcgg cttgagccag gtcaggaacg ggcggctgcg
     1921 tcccacgatc atctcgagca gcagggactg gctctcctcg agcgccatgc cgcggtcgcg
     1981 gccgaccggc tggtcgcgcc aggccgccgg caggcccagg tcgtacatcg cctggccggt
     2041 ctcgtgcagc acgcccatca gggccgtgaa cggctcctgc acgtcgaagc gtgtcgtgac
     2101 gcgcacgtcg ccccagacgc cttcggtgaa cgcgtggctg gcctcgtcga ggcggccgcg
     2161 gtggaacggg aaaccgagcg cgcgcatgac ctcgagcacg agcgcgcgct gcttcgagac
     2221 gctcaccttg ccggcgagcg gcagcggcgg ccgcgtcgac tgcaggtcga tcgcgtcgcg
     2281 gatcagtgac ggcagccggc ggttgaccac gcggaacagc tggtcgatgt cggcgctcag
     2341 gaatccgggg ctgaagccat cgatcagggc gtcgtagggc gcgagcccga gcgccttcga
     2401 cagcacctgc gccttgtcgc gcgtcagcgc cacgacctcc tcgaggtgcg gcgcaaaaag
     2461 cgggaattcg ttttcctgtc gcgcctcgag ccagcgcact tcggcgagcg cggtcgcgcg
     2521 cgcgaggcgc gtgacgagcg tcaggggtgt cgcgatcgcc cggtcgcgct cccggcgcat
     2581 ctcgcgcagg ttggcgagct gccagtcctc gagctgcgcc tggttggcct gcgcccgctc
     2641 gatcagccgc gacaccttcg gcgaggtgag cagcgcgtgg cactcggaat cgagcgccgc
     2701 gagctgctcg ccgcgcacgt ctgcgctgcc gcgcggcatc atcacggcgg cgtcccagcg
     2761 cagcaggctc gctgcgccac ggaacgcgtg gagcctgcgg aactcctgct cgagctgctg
     2821 gtacggactc tgtgccatga ctgtttggag acgcccatcc tgggccgcga gtagcgctct
     2881 ccgcctcgga gcgggaacgg tcacgaacca gccgtgggga cgggcgacga ttgtagcaaa
     2941 gccgtcaccg cggacgagct gccgtgcgga cgggaagagt caattttact tattattcaa
     3001 taatttagac gacaggtggc cggtcctgca caccgccggc ccgggctgcc aggccctccc
     3061 gtagacgatc gagtcgctgc gcgacggcct gcggcgagcc ggtgttgcgg gcgaccagcg
     3121 cgtacacggc cggcaccacc accagcgtca gcagcacgga gatcacgacg ccgtagaaca
     3181 cgacgacgcc gatcgagctg agcgattccg cgcccgcgcc cgacgccagc atcagcggca
     3241 gcgcgccgaa cgcggtgcag aagctggtca tcagcaccgg ccgcagccgg atcgccgccg
     3301 cctcgatgac ggcctcgcgg tattcgacgc cgcggtcacg cagctggttc gcaaactcga
     3361 cgatcaggat gccgttcttg gcggcgagac ccaccagcat gatcgcggcg atctgggtga
     3421 acacgttgat cgacatgccg tacaaccaca gcccgaccag cgcgccgatg accgccagcg
     3481 gcaccgtcgt gatgatgatg aacgggtgca ggaagctctc gaacagggcg gcgagcacca
     3541 ggaacacgat caccagcgcg agcacgaaca tccagtagag ctgcgtgccc gactgcttga
     3601 actcgcgcga ctggccgtcg taggcgagct tgaccgaggg cggcatctcg gtctgcacca
     3661 cttcttcgac gtagcgcagc gcctcgccga gcgtgtggcc cggcaccagc gccgcgctga
     3721 ccgtgatcga gcgcagtcgg tcgaagcggt tcagccgcat cggcccggcg agctcggtca
     3781 ggcgcaccag gttggcgagc ggcacgaggt cgccggtgcg atccgaacgc acgtagaggt
     3841 tgtcgaggtc ggtcggcgtg ccgcgatcct cgacgcggcc ctgcaggatc acgttgtact
     3901 cgcggtcgcg gtcgacgtag gtcgtgacga tccgcgaccc gagtaccgtc tcgagcgtgc
     3961 gcgacacggt ctgcagcgac acgccgaggt cggccgcgcg gttgcggtcg acggccacct
     4021 cgatctgcgg cttgcgctcc tcgtagttcg actggacgtt ggtgagcccc ggatgcgcgc
     4081 tcatgcgctg catcagcagg tcacgccatt gaatcaggtc ggcgtactcc gtgccctgca
     4141 gcacgacctg caccggggaa ccgccgccga cgccccatcc cccgggcacg ttcacgaacg
     4201 cccgcacgcc cggcagcttg ttgaactcgg cgcgtatggc ctgcgcgatc tggtccgcgg
     4261 accgctcgcg ctcgtcccag ggcgccagca gcatgaagcc acgcgcctcg ttcatctggc
     4321 cgccgccgcc gccgaagccg cccggcacgc ggccgttcag gcgcaccacc tcgccggtcg
     4381 cgcgctcccg ggcgacgatc tcctcgacct gccgcaggtg acgatccatg tagtcgagcg
     4441 acgcgccctc cggaccgttc aggccgatca tcaggaaccc gcggtcttcc gtcggggcga
     4501 gttccgccgg cagcacgcgc aacagcagcg ccgccacggc ggtcgccgcg agggtcgccc
     4561 cgaccacgag ccacgggcgc cgcatcgcgc ggcgcaggcc gcggtcgtac caggcggaca
     4621 ggcgccggaa gaattcgtcg accttctcgg ccatgcgcga gcgctgcgcg ccgcccgaaa
     4681 acagcttcga catcatcatc ggcgtgagcg tcagtgcgac gagcgccgag aatgcgaccg
     4741 cggcggcgat cgtgaagccg aactcgcgga acagcttgcc gacgttgccc tcgaggaacg
     4801 acatcggcag aaacacggca accagcaccg cggtcgtggc gagcaccgca aagccgatct
     4861 cgcgcgagcc gtcgaccgcc gcgaccagcg ccggctcgcc gttctccatg cgccggtaga
     4921 tgttctcgag cacgacgatt gcgtcgtcga cgaccagccc gatcgcgagc accaggccga
     4981 gcagcgtcag ggtattgatc gagatgccga gctaggccat gacgatgaac gcggcaacga
     5041 tcgacaccgg gatcgtcacg gccggcacca gcgtcgcgcg ggcgttgccg aggaacaggt
     5101 agatcacgac gatcacgagg cccatcgcga tcacgagcgc acggacgact tccttcatcg
     5161 aggcgtcgat gtacatggac cggtccaggt tgatctcgag acgcgtgccg ggcggcaggt
     5221 ccggcaggat ccgttcgcgc tcttcgcgta cggctcgcgc gacctcgagc gtgttcgcct
     5281 tggagatctg ctcgaccccg atgctgatgc cgggcacgcc gttgctgcgc gcgacggtgc
     5341 gctcgttctc ggccgcgagc cgcacgtcgg cgacctcgcc gagacgcacc aaatagccgc
     5401 cctcgccacg accgaccacg agctcgcgga aatcctgctc ggtgttgagg ccggtctgcg
     5461 tgcgcagcgt cagct
//
LOCUS       DASPFR010000177         8501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1150919, whole genome shotgun sequence.
ACCESSION   DASPFR010000177 DASPFR010000000
VERSION     DASPFR010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150919"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VD737_04485"
     CDS             complement(<1..471)
                     /locus_tag="VD737_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HXW09848.1"
                     /translation="MSRRRVVVTGLGIVSPVGSTVASAWDGIVNGRSGIGPITKLDVS
                     AFPARIGGEVRDFDAEAYMSVKDVRKFDPFVPFGFASAVQAIKDSGVEVTEANAARIG
                     VAIGAGIGGLSTIEENTAKWLEAKSPRRISPFFIPGSIINAAAGQVSIHYGLRGP"
     gene            complement(556..798)
                     /gene="acpP"
                     /locus_tag="VD737_04490"
     CDS             complement(556..798)
                     /gene="acpP"
                     /locus_tag="VD737_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019752224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXW09849.1"
                     /translation="MSSIEQRVKAIVAEQLGVKEEEVTNESSFVDDLGADSLDTVELV
                     MALEEEFETEIPDEDAEKITTVQQAIDYINERRSQA"
     gene            complement(919..1662)
                     /gene="fabG"
                     /locus_tag="VD737_04495"
     CDS             complement(919..1662)
                     /gene="fabG"
                     /locus_tag="VD737_04495"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HXW09850.1"
                     /translation="MTLANEIAFITGATRGIGRAIALELGRRGARVVGTATTEAGARA
                     IDEYLQAAGITGRGVVLNVADGAAVEGCFKAVEGAEGTPSILVNNAGVTRDGLLMRMG
                     ADDWQTVLDTDLSAVYRTCKAVMRGMMKARKGRIVNIASVIGVMGNAGQTNYAAAKAG
                     MIGFSKSLAREVGSRNITVNVVAPGFIVTDMTEALGEAQKTALLGQVPLGRLGSPEDV
                     AHAVAFLASPEASYVTGETLHVNGGMYMI"
     gene            complement(1659..2609)
                     /gene="fabD"
                     /locus_tag="VD737_04500"
     CDS             complement(1659..2609)
                     /gene="fabD"
                     /locus_tag="VD737_04500"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HXW09851.1"
                     /translation="MSLAFVFPGQGSQSVGMLADLGGTEPVVRQTFDEASEVLGYDLW
                     ALCQQGPEAELGATERTQPAMLAAGVATWRVWLAKGGPRPVAMAGHSLGEYSALVCSD
                     ALEFRAAVDLVRYRGQVMQQAVPVGQGAMAAILGLEDAELEAACREAAQGEVVEAVNF
                     NAPGQTVIAGHASAVQRAIDGARARGAKRAVLLPVSVPSHSRLMVGAAEQLATRLASV
                     EVRLPNVPSVYTVDVRTHASPDGIRQSLKEQLYKPVRWADTVRAMIAGGCTTFVECGP
                     GKVLTSLNRRIERKPELKMLALEDGASIAAALAACAEATS"
     gene            complement(2659..3273)
                     /gene="rrtA"
                     /locus_tag="VD737_04505"
     CDS             complement(2659..3273)
                     /gene="rrtA"
                     /locus_tag="VD737_04505"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HXW09852.1"
                     /translation="MDLARLGHADVRAWRAPLGLAVLLVLVQMSGTLSSGTSLRYDRD
                     AIVAGGEWYRLVSGHLYHYDSTHLAWNLAGLALVAWLFVREYAPVQWLAILLGSTVAV
                     DLGFLVFEPQLAWYVGFSGVLHGLAAAGLVSWWRRYRDAVTVIASTVLALKLGWEHAV
                     GALPFTSGTLSIPVVHAAHTYGAVGGAVAGWLLGLRRPRPAPSL"
     gene            3345..3806
                     /gene="gspG"
                     /locus_tag="VD737_04510"
     CDS             3345..3806
                     /gene="gspG"
                     /locus_tag="VD737_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HXW09853.1"
                     /translation="MRFASRFTALRAAGRRAAGFTLIEIMVVVVILGILAALVAPNVI
                     RRIDDARVTKARQDIRAYETALNLYRMDNFRYPNTEQGLQALVQRPADPNIRNWKEGG
                     YIDGIKKDPWGNDYAYIAPGTRGEYDLYTLGADGQPGGEGPDADIGNWNIE"
     gene            3811..4365
                     /gene="gspH"
                     /locus_tag="VD737_04515"
     CDS             3811..4365
                     /gene="gspH"
                     /locus_tag="VD737_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HXW09854.1"
                     /translation="MRPRPAARRRASGFTLLELLVVVVIIGIITSMAVISVNVLGGDH
                     EMQQEAERLQAILIQSREDALLQSRDLGLRLDETSFEFLEYDGRTERWRPVLGDPLLR
                     ERTLPPGLRVRLRVEDRDVQLKPRQPETERDPIYPQVILQASGEIVPFDVIFSRDGTD
                     EQRRVSGTVEGRIELHDDARESRP"
     gene            4500..5045
                     /gene="gspI"
                     /locus_tag="VD737_04520"
     CDS             4500..5045
                     /gene="gspI"
                     /locus_tag="VD737_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HXW09855.1"
                     /translation="MSPFSGFTLIEVLAALVIVALGMLGAITAVNQSARNGTYLREKT
                     LAHWVAMNVITERRLQASPPDVAESNGEVEFANARWRWTLRVTQTEVQSLRRMDVSVA
                     LATAPEDSSLARLTGFYGTALSTASGGSVSWAGTGAPGSGGEDDGSDDEADDGGRPRA
                     DRPVPDEPPEDDVPVDPEAED"
     gene            5047..5667
                     /gene="gspJ"
                     /locus_tag="VD737_04525"
     CDS             5047..5667
                     /gene="gspJ"
                     /locus_tag="VD737_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HXW09856.1"
                     /translation="MSSRQRTAGFTLLEVLVAIAIFAIVGVMSLTGYTQLQRQSEYQE
                     QRLERVREVQRAVQTIAQDLTQIEPRPIREPIGEQHIPALLAGDSVEYKLEFTRAGWS
                     NTAGLPRPTLQRVAYRLDQDGLWRDYWRVLDRTQTAEPTRQNLLKGVRSVTFRYMTPA
                     REWVDRWPVLEGQSALEQERARPAAIEVTLELDDWGVLRRLVEVSG"
     gene            5667..6632
                     /gene="gspK"
                     /locus_tag="VD737_04530"
     CDS             5667..6632
                     /gene="gspK"
                     /locus_tag="VD737_04530"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HXW09857.1"
                     /translation="MRARARPATPPPRQRGVALITAVLVVALATMLAINVTFRGMVDQ
                     RRSANLFAFDQGFEVALGAEAWAADFLRKDAQETQQDHLGETWAQSMPPLPIDEGVGT
                     VEGRLEDLQGRFNLNNLVFSDGTPNALAISQLERILAMVDLEPAWAVAIADWIDGDVN
                     PGFPDGAEDSVYTGMDPPHLAANMPITRTSELLVLPEFGIERYRRLQPYVTALPAGTK
                     LNVCTAPGVVLDALAEDQRQFSLNAQDLATRRKDACFPTVEDIRGTLGQAGYDQVKDT
                     IGESSSYFRATIWVTIGTTQFTLYSLLARGGAGSIRPLLRSFGAE"
     gene            6637..7866
                     /gene="gspL"
                     /locus_tag="VD737_04535"
     CDS             6637..7866
                     /gene="gspL"
                     /locus_tag="VD737_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HXW09858.1"
                     /translation="MTETLVIRLRSTEDAPASWLIIDGNGARSGPVVNGPIADALSLS
                     QSRRTVIVLPAAEVTLAEPDLPPVRGASRIAQAVPFALEEQLASDLENLHFAVGARNP
                     SASATPVAIVARSVLDRWQAVWEAAGIHPDAAYAESSLIPAAPNATVLVLDEGTLHVK
                     RRAAVAYALDALTLATSLELALATTGDGGEHVTFYAGTADYESNKDVIEALRERTATL
                     QVKLLPDGVLPLFAAQLPTARAVNLLQGAYAPPSSFGSQLRQWRLPAALAVATLLVFL
                     GAQGVKLWQLRQAEQKLNAEIAQTFESILPGQPMVDPRAQVQGVLARAGGGAGALLPA
                     VSLLAQAVAQAPATRVEGMNFRGGVLELRVVAPTIEALDGIRETMSSRGATVELQSTN
                     PRNQQVEGRLHVRLGAA"
     gene            7863..8360
                     /gene="gspM"
                     /locus_tag="VD737_04540"
     CDS             7863..8360
                     /gene="gspM"
                     /locus_tag="VD737_04540"
                     /inference="COORDINATES: protein motif:HMM:NF016493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HXW09859.1"
                     /translation="MNLDTVRDWLGNLSTRERNLVYGAAVLAALALLYLVIVLPFTTT
                     TSRMTARVEQKSADLAWMRAQAPHAAAAAGLAQSRSGESLVVIVDRTARQAGLGATLR
                     DQSPSGNAGLRLRMEAAPFDTLVTWLAVLQQQHGVGIEAATIDAAGPGLVNATLSLTH
                     AGAAG"
BASE COUNT         1322 a         2984 c         2910 g         1285 t
ORIGIN      
        1 cgggccgcgc agtccgtagt ggatcgacac ctgtccggcg gccgcattga tgatgctgcc
       61 cgggatgaag aacggcgaga tcctgcgcgg cgactttgcc tcgagccact tggccgtatt
      121 ttcctcgatc gtcgacaacc cgccgatgcc cgcgccgatc gcgacgccga tacgcgctgc
      181 gttggcctcg gtcacctcga cgccggagtc cttgatcgcc tgcacggccg acgcgaagcc
      241 gaacggtacg aacgggtcga acttgcgcac gtccttgacg gacatgtagg cctccgcgtc
      301 gaagtccctg acttcgccgc cgatgcgcgc cgggaacgcg ctcacgtcga gcttggtgat
      361 cggcccgatg ccgctacgac cgttgacgat gccgtcccag gccgacgcca cggtgctgcc
      421 caccggggac acgatgccta aaccagtgac gacgaccctg cgcctggaca agctgagtcc
      481 ccccggatga gctgctggaa aacggtggtc ggagccgtga ccgccttccg tggcggaccg
      541 gcgacggggc gcagctcagg cctggctgcg acgctcgttg atgtaatcga tggcttgctg
      601 gacggtcgtg atcttctcgg cgtcctcgtc cggaatctcg gtctcgaatt cttcttcgag
      661 cgccatgacg agttcgacgg tgtcgagcga gtccgcaccc agatcgtcga cgaaggagga
      721 ctcgttggtg acctcttcct ccttgacgcc cagttgctcg gcgacgatcg ccttcactcg
      781 ctgttcaata ctgctcattg tcgttgagat cctgtgatag gaaatgaacc cgatcgcggg
      841 ccgccctggg tcggcccgac gtggggcgcg tattctatgt taaggctcca cgggggtcca
      901 attgacgcct ttttcttttc agatcatgta catgccgccg tttacgtgca gcgtttcacc
      961 ggtgacgtag gacgcttccg gcgaggccag gaacgccacc gcgtgcgcga cgtcctccgg
     1021 tgatccgaga cggccgagcg gcacctggcc gagcagcgcc gtcttctgcg cctcgcccag
     1081 cgcctcggtc atgtcggtga cgatgaagcc gggcgcgacg acgttgacag tgatgttgcg
     1141 ggaaccgacc tcgcgggcca ggctcttcga gaaaccgatc atgccggcct tggccgcggc
     1201 gtaattggtc tggccggcgt tgcccatgac cccgatcacg gaggcgatgt tgacgatgcg
     1261 gcccttgcgc gccttcatca tgccgcgcat cacggccttg caggtccggt agaccgcgct
     1321 caggtcggtg tcgagcacgg tctgccagtc gtcggcgccc atgcgcatca gcaggccgtc
     1381 gcgcgtgacg cccgcgttgt tgaccaggat cgacggcgtg ccctcggcgc cctcgaccgc
     1441 cttgaaacaa ccctccaccg ccgcgccgtc ggccacgttc aggacgacgc cgcggcccgt
     1501 gattccggcc gcctgcaggt actcgtcgat cgcccgggcg ccggcctcgg tcgtcgccgt
     1561 cccgacgacg cgcgcgccac gacggcccag ctcgagcgcg atggcccggc cgatgccacg
     1621 cgtggcgccg gtgatgaagg caatttcgtt cgcgagcgtc atgacgtggc ctccgcgcag
     1681 gcggccagcg cggccgcgat cgacgcgccg tcctccagcg cgagcatctt gagttcgggc
     1741 ttgcgttcga tgcggcggtt gagcgacgtc agcaccttgc cgggcccgca ctcgacgaac
     1801 gtggtgcagc cgccggcgat catcgcgcgc acggtgtcgg cccagcgcac cggtttgtac
     1861 agctgttcct tgagcgactg gcggatgccg tcggggctcg cgtgcgtacg gacgtcgacg
     1921 gtgtagacgg acggaacgtt cggcaggcgc acctcgacgc tggcaagccg cgtggcgagc
     1981 tgctccgccg cccccaccat cagccggctg tgcgagggca cgctgacggg cagcagcacc
     2041 gcgcgcttgg cgccgcgcgc ccgggcgccg tcgatcgcgc gttgcaccgc gctcgcgtgc
     2101 ccggcgatga cggtctggcc cggcgcgttg aaattcacgg cctcgacgac ttcgccctga
     2161 gcggcttcgc ggcacgcggc ctcgagttcg gcgtcttcga ggccgaggat cgccgccatg
     2221 gcgccctgcc cgaccggtac ggcctgctgc atgacctggc cgcggtagcg cacgaggtcc
     2281 accgccgcgc gaaactcgag cgcgtccgag cacaccagcg cggagtactc gccgaggctg
     2341 tgtcccgcca tcgcgaccgg acgcggcccg cctttcgcga gccacacgcg ccacgtggcg
     2401 acgcccgccg cgagcatcgc cggctgcgtg cgttcggtgg cgccgagctc ggcctcgggt
     2461 ccctgctggc agagcgccca caggtcgtag ccgagcacct cgctcgcctc gtcgaaggtc
     2521 tgccgcacga cgggctcggt cccgccgagg tcggcgagca ttccgaccga ctgcgacccc
     2581 tggccgggaa agacgaatgc caggctcatg tgcttgcgat gcctcgcttg gggagcggcc
     2641 gtccggccgg acccgcgatt ataacgacgg cgcaggcctg ggccggcgca ggccgagcag
     2701 ccagcccgcg acggcgccgc cgaccgcccc gtaggtgtgc gctgcgtgca cgacggggat
     2761 cgacagcgtt ccggacgtga acggcagcgc gcccacggcg tgttcccagc cgagcttgag
     2821 cgcgagaacg gtcgaggcga tcaccgtcac cgcgtcccgg tagcgccgcc accagctcac
     2881 caggccggcc gccgcgagcc cgtgcaggac gccggagaac ccgacgtacc acgcgagttg
     2941 cggctcgaat acgaggaacc cgaggtcgac ggcgacggtc gaacccagca ggatcgcgag
     3001 ccactgcacc ggcgcgtact cgcgcacgaa gagccacgcg acgagcgcga gcccggcgag
     3061 gttccaggcg aggtgcgtcg agtcgtagtg atagaggtgc cccgagacca gccggtacca
     3121 ctcgccgccc gccacgatcg catcgcggtc gtagcgcagc gacgtcccgg aactgagcgt
     3181 gccggacatc tgcaccagca cgagcagcac cgcgagcccg agcggcgcgc gccatgcgcg
     3241 tacgtcggcg tgtccgaggc gggcgaggtc catgcagcgt gcggggccgg ggtcggggtg
     3301 tttgctatga ttcgtacaat ttaccgccag ccacggttcg acacatgaga ttcgcaagtc
     3361 gcttcactgc ccttcgcgcc gccgggcgcc gggccgccgg tttcacgctc atcgagatca
     3421 tggtcgtcgt ggtgatcctc ggcatcctcg ccgcgctcgt cgcgccgaac gtgatccgtc
     3481 gcatcgacga cgcccgggtg accaaggcca ggcaggacat ccgcgcctac gagaccgcgc
     3541 tcaacctgta ccgcatggac aacttccggt acccgaacac cgagcaggga ctgcaggcgc
     3601 tcgtgcagcg gcccgccgac ccgaacatcc gcaactggaa ggaaggcggc tacatcgacg
     3661 gcatcaagaa ggacccgtgg ggcaacgact acgcctacat cgcgcccggc acgcgcggcg
     3721 agtacgacct ctatacgctc ggggccgacg gccagccggg cggcgaaggc ccggatgccg
     3781 acatcggtaa ctggaacatc gaataacgcc ttgcggcccc gtccggccgc ccgtcgtcgc
     3841 gcctcgggct tcacgctgct cgagttgctc gtcgtcgtcg tcatcatcgg catcatcacg
     3901 tcgatggcgg tgatctcggt caatgtgctc ggcggcgacc acgagatgca gcaggaggcc
     3961 gagcgcctgc aggcgatcct gatccagtcg cgcgaggacg ccctcctgca gagtcgcgac
     4021 ctgggactgc gtctcgacga gacgagcttc gagttcctgg aatacgacgg ccgcaccgag
     4081 cgatggcgcc ccgtgctcgg cgatcccttg ctgcgcgagc gcacgctacc gcccgggctg
     4141 cgcgtgcgac tgcgcgtcga ggaccgcgac gtgcaactga agccgcggca gcccgagacc
     4201 gagcgcgacc cgatctatcc gcaggtgatc ctgcaggcct ccggggagat cgtcccgttc
     4261 gacgtgatct tctcgcgcga cggcaccgac gagcagcgtc gcgtcagcgg caccgtcgag
     4321 ggccgcatcg agctgcacga cgacgcgcgg gagagccggc cgtgaacgga aaatggggac
     4381 actcctattt tccgggaaaa tggggacatt cctattttcc gcgggtaccc cagccacatg
     4441 cgcgcagatc ccggcagcgg aaaatcggag tgtccccatt ttccggaagg aaaataggag
     4501 tgtccccatt ttccggcttc acgctgatcg aggtgcttgc cgcgctggtg atcgtcgcgc
     4561 tcggcatgtt gggcgcgatc accgcagtca accagtcggc gcgcaacggc acgtacctgc
     4621 gtgagaagac gctcgcgcac tgggtcgcga tgaacgtgat caccgagcgc cggctgcagg
     4681 cgtcgccgcc ggacgtcgcc gagagcaacg gcgaggtcga gttcgcgaac gctcgctggc
     4741 gctggacgct gcgcgtcacg cagaccgagg tgcagtcgct gcggcgcatg gacgtgagcg
     4801 tggcgctcgc caccgcaccg gaggattcgt cgctggcgag gctgaccggc ttctacggca
     4861 ccgcgctcag cacggcgagt ggcggcagcg tgagctgggc cggcacgggc gcgccgggca
     4921 gcggtggcga ggacgacggc agcgacgacg aagccgacga tggcgggcgc cctcgggccg
     4981 atcggcccgt cccggacgaa ccacccgaag acgacgtgcc ggtcgatccg gaagcggagg
     5041 actgaggtgt cgtcgcgcca gcgcaccgcc gggttcacgc tgctcgaagt gctcgtcgcg
     5101 atcgcgatct tcgcgatcgt gggggtcatg tcgctgaccg gctacacgca gctgcagcgg
     5161 cagagcgaat accaggaaca gcgactcgag cgcgtgcgcg aggtgcagcg cgccgtgcag
     5221 acgatcgcgc aggatctcac gcagatcgag ccccggccga tccgcgagcc gatcggcgag
     5281 cagcacatac cggcgctgct cgcgggcgat tcggtcgagt acaagctcga attcacgcgc
     5341 gccgggtggt cgaacactgc gggcctgccg cggccgacgc tgcagcgcgt agcctaccgc
     5401 ctcgaccagg acgggctctg gcgtgactac tggcgcgtgc tcgatcgcac gcagacggcg
     5461 gagccgacgc ggcagaacct gctgaagggc gtgcgatcgg tgacgttccg ctacatgacg
     5521 ccagcgcgcg aatgggtcga tcgctggccc gtgctcgagg ggcagagcgc gctcgagcag
     5581 gagcgcgcac gtccggccgc gatcgaggtc acgctcgaac tggacgactg gggcgtgctg
     5641 cgtcgactgg tcgaggtgtc gggctgatgc gcgcgcgtgc ccgccccgcc acaccgccgc
     5701 cgcgccagcg cggcgtcgcg ctgatcaccg ccgttctggt cgtcgcgctc gcgacgatgc
     5761 tcgcgatcaa cgtgacgttc cgtggcatgg tggaccagcg tcgcagcgcc aacctgttcg
     5821 cgttcgacca gggattcgag gtggcgctcg gcgcggaggc ctgggcggcc gatttcctgc
     5881 gcaaggacgc gcaggagacg cagcaggatc acctcggcga gacctgggct caatcgatgc
     5941 cgccgctgcc gatcgacgag ggcgtcggca cggtcgaggg acggctcgag gacctgcagg
     6001 ggcgcttcaa cctcaacaac ctcgtcttct cggacggcac gccgaacgcg cttgcgatca
     6061 gtcagctcga gcgcatcctc gcgatggtgg acctcgagcc cgcgtgggcc gtcgcgatcg
     6121 ccgactggat cgacggcgac gtcaacccgg ggtttccgga cggcgcggag gacagcgtct
     6181 acacgggcat ggatccgccg cacctcgcgg ccaacatgcc gatcacacgc acgagcgagc
     6241 tgctggtgct gcctgagttc ggcatcgaac gctatcgacg gctccaaccc tacgtcaccg
     6301 ccctgcccgc cggcaccaag ctcaacgtct gcacggcacc gggcgtcgtg ctcgatgcgc
     6361 tcgcggagga ccagcgccag ttcagcctca atgcacagga cctcgcgact cgtcgcaagg
     6421 atgcgtgttt tccgacggtg gaggacatcc gcggcacgct cggccaggcc ggctacgacc
     6481 aggtcaagga cacgatcggc gaatcgagtt catatttccg cgcgacgata tgggtcacta
     6541 ttggcactac ccagttcacc ctgtacagtc tgctcgcgcg tgggggcgcc ggctcgatca
     6601 ggcccctgct gcgcagcttc ggagccgaat gatccaatga ctgaaaccct ggtgatccga
     6661 ctccggtcga ccgaagacgc gccggcgtcc tggctcatca tcgacggcaa cggcgcccgc
     6721 tccggccccg tcgtcaacgg acccatcgcc gacgccttga gcctgtcgca gagccgacgc
     6781 acggtgatcg tgctgccggc ggccgaggtg acgctggcgg agcccgacct gcctcccgtg
     6841 cggggcgctt cgcgcatcgc ccaggccgtg cctttcgcgc tcgaggagca actcgcgtcc
     6901 gatctcgaaa acctgcactt cgcggtcggc gcgcgcaacc cctcggcgag cgcgacgccc
     6961 gtggccatcg tcgcgcgcag cgtgctcgat cgctggcagg cggtctggga agcggccggc
     7021 atccatccgg acgccgcgta cgccgagtcg tcgctgattc cggcggcgcc caatgcgacg
     7081 gtgctcgtgc tcgacgaagg cacgctgcac gtcaagcgtc gggccgcggt cgcgtacgcg
     7141 ctcgatgcac tcacgctcgc gacgtcgctc gagctcgcac tcgcgacgac gggcgacggc
     7201 ggcgaacacg tcacctttta cgccggcacg gccgactacg agtccaacaa ggacgtcatc
     7261 gaagcactgc gcgagcgcac ggccacgctg caggtcaagc tgctgccgga cggcgtgctg
     7321 ccactcttcg ccgcgcagct gccgacggcc cgtgccgtga acctgctgca gggggcctac
     7381 gcaccgccct cctcgttcgg gtcgcagctg cgccagtggc gactgccggc ggccctggcg
     7441 gtcgcgacgc tgctcgtgtt cctcggtgcg cagggcgtca agctctggca gttgcgccag
     7501 gccgagcaga agctcaacgc cgagatcgca cagacgttcg agtcgatcct gcccggccag
     7561 ccgatggtcg atccgcgtgc gcaggtgcag ggcgtgctcg cgcgtgccgg aggtggcgca
     7621 ggcgcgctgc tgccggcggt gtcgctgctg gcccaggccg tggcgcaggc gcccgcaacg
     7681 cgcgtcgagg gcatgaattt ccgcggcggc gtgctcgagt tgcgcgtcgt cgcgccgacg
     7741 atcgaggcgc tcgacggcat ccgcgagacc atgagcagtc gtggcgccac cgtcgagctg
     7801 cagtcgacca atccgcgcaa ccagcaggtc gaaggccgcc tgcacgttcg cctgggggcc
     7861 gcatgaacct cgacacggtt cgcgactggc tcggcaacct cagcacgcgt gagcgcaatc
     7921 tcgtctacgg cgctgcggtg ctggcggccc ttgcgctgct gtacctcgtg atcgtgctgc
     7981 cgttcaccac aaccacgtcg cgcatgacgg cacgcgtcga gcagaagtcc gcggacctcg
     8041 cgtggatgcg cgcccaggcg ccccacgcgg cggcggccgc cggcctcgca caatcgcgct
     8101 ccggcgaatc gctggtcgtg atcgtcgatc gcaccgcgcg ccaggccgga ctcggcgcga
     8161 cgctgcgcga ccagagcccg agcggcaatg cggggctgcg actgcgcatg gaagcggccc
     8221 ctttcgacac gctcgtcacc tggctcgccg tcctgcagca gcagcacggc gtcggcatcg
     8281 aggcggccac gatcgacgcc gccggcccgg ggctcgtcaa cgcgacgctg agcctcacgc
     8341 acgccggcgc ggcgggttga cggcgcagcc gaccaggctg agcgggattc ccccacgtga
     8401 agcatcggac cggctggctg gtcgcgctcg gcattctcgc cttgctcgcg ttcgcactgg
     8461 tcacgctgcc ggcgaccgtg ctgtccagct tcgcaggacg t
//
LOCUS       DASPFR010000178         4087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1517530, whole genome shotgun sequence.
ACCESSION   DASPFR010000178 DASPFR010000000
VERSION     DASPFR010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517530"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..3685)
                     /locus_tag="VD737_04545"
     CDS             complement(110..3685)
                     /locus_tag="VD737_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXW09860.1"
                     /translation="MSLHPLLKQQFDDARQEGGQLDLRRLLHAISTAYAEWDEERRGV
                     VRSMRLLADETTAFTREVRESAAAQLQAILDHVKDAILTVDDTGRIETLNTTGERVFG
                     YGENDVRGQKLDLLIPSLARKPRIVEALEEFATAVEDTQADLAPRETRGRHKNGTLFD
                     AEIGVSKVRLDRREMYIVCLRETTDRKAAEAAIRESEARYRTLVEHAPEAIVVLDMDL
                     GHFVECNENAVRFFKMTREELLHSGPQKISPPYQSDGSPSFGVARGYLDRALAGEAPN
                     FEWLHRDALGHDIPCEVRLVRLPSSGRRLIRGSITDITERKRNELLAVGDRRVFERIT
                     SHADLMTTLEAVTETAEKVTPDAICAVSVYEPEANVLFHVAGTRLPQPYLNAKRRVEI
                     GPRNGSCAAAVFLQRQVIVAEITRDALWEHVRVPAVEAGLRACWSTPVRASDGRVLGT
                     VALYFRQPRSPLRRDFELMSRLTALAGIAIERKRSEEALRRSEAQYRSLFENVIEGVY
                     RSTADGRFEAVNPALVQMLGYDSADELLAIDDNRVIYAHASERDNVISTLHRDGLVRG
                     AESQLRRRDGTLVTVQENARVIRGADDGIVGYEGTLTDITERKRAELQLYEEKEKAQV
                     TLQSIGDAVITTDASGRVEYLNPVAEDLTGWDSREAYGRPIGEVFHVVSEATRQPVDS
                     PIVRCLREGRVVDMVEPSVLINRRGQEISVQDSAAPIRDRGGRLIGVVMVFHDVSQER
                     RLQRALSYQATHDVLTGLINRREFELRLTDALHTARADPSIRHVVMFLDLDQFKVVND
                     TCGHAAGDRLLKQITSVLQTRIRATDTLARLGGDEFGVLLQDCTLETAQRIGEELRQA
                     IRDFRFSWQDRVMNVGVSIGLAEMNAEHETLSTIMSAADVACYSAKESGRNRVHTYSE
                     GQAPERLREMQWVSRINQACDEERFELYFQPIVPIRHGVETTRQFELLLRMRDEAGRL
                     VLPNEFIPAAERFNLMPAIDRWVVRQACRTLAHRRGTDGSIPPYCLTINVSTTTINDE
                     QFLDHVIAEMAAAEVSPGALCFELTETTAMTSLVAATHFIRELRKRGVRFSLDDFGSG
                     LSSFLFLKNLPVDFVKIDGQFVHNVAQDAIDRSMVDAITQIAGAMGICTVAERVDSAE
                     VLEQLAIVGVQYAQGHYIASPQPVSVLHELLASPMTVGEVGRKAG"
BASE COUNT          590 a         1374 c         1443 g          680 t
ORIGIN      
        1 ctcgagcggg aaagggaaat tcaggtacta cgttcgagcc gcgaacgcgt caggggtttc
       61 ggcgttcgtt gcgtcgccaa cggtgacagt gacgggaggg taggagccct cacccggcct
      121 tgcggccgac ctctcccacc gtcatgggcg aggcgagcag ctcgtgcagc acgctcaccg
      181 gctgcggcga cgcgatgtag tgcccctgcg cgtactgcac gccgacgatg gcgagctgct
      241 cgagcacctc ggcggagtcg acccgctcgg cgaccgtgca gatgcccatc gcgcccgcga
      301 tctgcgtgat cgcgtccacc atgctgcggt cgatcgcgtc ctgtgcgacg ttgtggacga
      361 actgcccgtc gatcttgacg aagtcgaccg gcaggttctt gaggaacagg aacgacgaca
      421 ggccgctccc gaagtcgtcg agcgagaagc ggacgccgcg cttgcgcagc tcccggatga
      481 agtgcgtggc ggcgacgagc gacgtcatcg ccgtcgtctc ggtcagctcg aagcacagcg
      541 cacccgggct gacctcggcc gccgccattt ccgcgatcac gtggtcgagg aactgctcgt
      601 cgttgatcgt cgtcgtcgag acgttgatcg tcaggcagta cggcgggatc gagccgtccg
      661 tgccgcggcg gtgcgcgagc gtgcggcagg cctggcggac gacccagcga tcgatcgccg
      721 gcatcaggtt gaagcgctcg gccgcgggga tgaactcgtt cggcagcacc agccggccgg
      781 cctcgtcgcg catgcgcagc agcagctcga actggcgcgt cgtctcgacg ccgtgacgga
      841 tcggcacgat cggctggaag tacagctcga agcgctcctc gtcgcacgcc tggttgatgc
      901 gcgacaccca ctgcatctcg cgcaggcgct ccggcgcctg gccctcgctg taggtgtgga
      961 cgcggttgcg gcccgattcc ttggccgagt agcaggcgac gtcggccgcc gacatgatgg
     1021 tcgacagcgt ctcgtgctcg gcgttcatct cggcgaggcc gatcgacacg ccgacgttca
     1081 tgacgcggtc ctgccagctg aaccggaagt cgcggatcgc ctggcgcagc tcctcgccga
     1141 tccgctgcgc cgtctccagc gtgcagtcct gcagcagcac gccgaactcg tcaccgccta
     1201 ggcgcgcgag cgtgtcggtg gcgcggatcc gggtctgcag cacgctcgtg atctgcttga
     1261 gcagccggtc gccggccgcg tgcccgcagg tgtcgttgac gaccttgaac tggtcgaggt
     1321 cgaggaacat cacgacgtgg cggatcgacg ggtccgcccg ggcggtgtgc agggcgtcgg
     1381 tgagtcgcag ctcgaactcc cggcgattga tcagtccggt gagcacgtcg tgggtcgcct
     1441 ggtaggagag cgcgcgctgc aggcgacgct cctggctcac gtcgtggaac accatcacga
     1501 cgccgatcag gcgaccgccg cggtcgcgga tcggtgccgc ggagtcctgc accgagatct
     1561 cctggccgcg ccggttgatc agcaccgacg gctcgaccat gtcgacgacg cggccttcgc
     1621 gcagacagcg cacgatcggg ctgtcgaccg gctggcgggt ggcctcggag acgacgtgga
     1681 acacctcgcc gatcggccgc ccgtacgcct cgcgtgaatc ccagccggtc aggtcctcgg
     1741 cgacggggtt caggtactcg acgcggccgc ttgcgtcggt cgtgatgacc gcgtcgccga
     1801 tcgactgcag cgtgacctgc gccttttcct tttcctcgta gagctggagc tcggcgcgct
     1861 tgcgctccgt gatgtcggtg agcgtgccct cgtagccgac gatcccgtcg tccgcgccac
     1921 ggatcacgcg cgcgttctcc tgcaccgtga ccagcgtgcc gtcgcgacga cgcagctggc
     1981 tctcggcgcc gcgcaccagc ccgtcccggt gcagcgtcga gatcacgttg tcgcgctcgc
     2041 tggcgtgcgc gtagatgacc cggttgtcgt cgatcgcgag cagttcgtcc gccgagtcgt
     2101 acccgagcat ctgcacgagc gcggggttga cggcctcgaa gcgcccgtcg gcggtcgagc
     2161 ggtagacgcc ttcgatcacg ttctcgaaca ggctgcggta ctgggcctcg ctgcgccgga
     2221 gcgcctcttc cgagcgcttg cgctcgatcg cgatgccggc gagggccgtc aggcgcgaca
     2281 tcagctcgaa gtcgcggcgc agcgggctcc gcggctggcg gaagtacagc gcgacggtgc
     2341 ccagcacgcg tccgtcggac gcgcgcacgg gcgtggacca gcaggcgcgc agcccggcct
     2401 cgacggcggg gacccgcacg tgctcccaca gcgcgtcgcg cgtgatctcg gcgacgatga
     2461 cctgccgctg caggaagacc gcggccgcgc atgagccgtt gcgcggcccg atctcgacgc
     2521 gacgcttggc gttgagatag ggctgcggca gccgcgtgcc cgcgacgtgg aacagcacgt
     2581 tggcttcggg ctcgtagacg gagaccgcgc agatggcgtc gggcgtgacc ttctcggcgg
     2641 tctcggtgac cgcctcgagc gtcgtcatca ggtcggcgtg gctcgtgatg cgctcgaaca
     2701 cgcgccggtc gccgaccgcc agcaactcgt tgcgcttgcg ttcggtgatg tccgtgatgc
     2761 tgccgcggat cagccgccgg ccggaggagg gcaggcgcac caggcggact tcgcacggga
     2821 tgtcgtgccc gagcgcgtcg cgatgcagcc actcgaagtt gggcgcctcg ccggcgagcg
     2881 cgcggtcgag atagccgcgt gcgacgccga acgacgggct gccgtccgac tggtagggag
     2941 ggctgatctt ctgcgggccg gagtgcagca gttcctcgcg cgtcatcttg aagaagcgca
     3001 cggcgttctc gttgcactcg acgaagtgcc cgagatccat gtcgagcacc acgatcgctt
     3061 cgggcgcgtg ctcgaccagc gtgcggtagc gagcctcgct ttcgcggatc gccgcttcgg
     3121 cggccttgcg gtccgtggtc tcgcgcaggc agacgatgta catctcacgg cggtcgagcc
     3181 gcaccttgct cacgccgatc tcggcgtcga acagcgtgcc gttcttgtgc cggccgcgag
     3241 tctcgcgggg cgccaggtcg gcctgcgtgt cctcgaccgc cgtcgcgaac tcctcgagcg
     3301 cctcgacgat gcggggtttg cgggcgaggg acgggatcag caggtcgagt ttctggccac
     3361 gcacgtcgtt ttcgccgtag ccgaagacgc gctcgccggt ggtgttcagg gtctcgatgc
     3421 gtcccgtgtc gtcgaccgtc aggatcgcgt ccttgacgtg gtcgaggatc gcctgcagct
     3481 gcgcggccgc gctctcacgg acctcgcgcg tgaaggcggt ggtctcgtcg gcgagcagcc
     3541 gcatcgagcg gacgaccccc cggcgctctt cgtcccactc cgcatacgcc gtgctgatgg
     3601 cgtgcagcaa ccggcgcaaa tcgagctggc caccctcctg ccgggcgtcg tcgaattgct
     3661 gcttgaggag cgggtgcagg ctcatcgggc gaaaacgggt ccttcagccc gcttcggcgg
     3721 gtgtccgcgc atggtaggtc cggaatggag ttttctgttg tgactagagt ctcaccgaac
     3781 cggcaggcgc tgggaaggac gagtacgcaa tatccgacga gcggacgcct gcgacgggcg
     3841 ggtggtcgac cgacacgttc atcgatcctg tcggcgcaat gacgccgcaa ggcattatgt
     3901 gaaagcgcgg ggctcggggt gcaccgaaaa ggtgccgcgg gccgtcacag cgtcgtcgac
     3961 acgaccccgg accgggcact ctcgatgccc gccgcgttgt acgaggcgat cgcgaagtac
     4021 caggtgccgt gctcgagacc ctggatctcc gctttcgaca cggtcgcgcc ccgcacgtcc
     4081 acgacct
//
LOCUS       DASPFR010000179         6688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1028657, whole genome shotgun sequence.
ACCESSION   DASPFR010000179 DASPFR010000000
VERSION     DASPFR010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6688
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028657"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="VD737_04550"
     CDS             complement(<1..1261)
                     /locus_tag="VD737_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HXW09861.1"
                     /translation="MTPSPGSACFHCGEPIPAGVRIHARVKGHEQPVCCHGCKAVAEF
                     ITGAGLDDYYRYRDQAGARADEPPRADRWSAYDRPELVERLTRAEPDGARSITVLLEG
                     LRCSACSWLADRALHQQAGVLDVSVNAATARARLVWDPTRVRLGDLLRVLEHVGLRPH
                     PLAGEPAEQLALLERRTALKRLAVAGFGMMQVMMFALPLYVGGVERGMHEFFRLVSML
                     ITVPVAIYAGWPFYVGAWHALRARTVSMDVPVTLGIVAAFTASVWNALSGRGEVYFDS
                     VTMFVFFLALGRYVEMVARHRAGSVADALARLAPVTARRVRGGQVEDVQALELRPGDH
                     LLVRTGEILAADGVVAEHVDGRIDESLLTGESTAIAKTRGSAVHAGTQNLGPPLEIEV
                     TAVGGATVLSGIVALLERAQAERPRLAR"
     gene            complement(1258..1455)
                     /locus_tag="VD737_04555"
     CDS             complement(1258..1455)
                     /locus_tag="VD737_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09862.1"
                     /translation="MSIDAARPWYRQAWPWLLIIPPAGAVIGGAITLYLAISRPDVLV
                     RTDCQRDGAITVCGEDAPRAP"
     gene            complement(1473..2855)
                     /gene="ccoG"
                     /locus_tag="VD737_04560"
     CDS             complement(1473..2855)
                     /gene="ccoG"
                     /locus_tag="VD737_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HXW09863.1"
                     /translation="MSEVEERVEGALYAARQKIYPREIQGRFQRLRVYAVWGLLGLYY
                     LLPWVPWQGRQAVLFDLPARQFHIFGLTLWPQDFYFLTWLLVIAALALFFFTALAGRL
                     FCGYACPQTVWTEVFIWMERWVEGSRNQQIKLAKAPWTRDKVLRKAAKQFLWIAFALW
                     TGFTFVGYFTPITELAGKLVGFSTGPWETFWVLFYGGATYVNAGHMREQVCKYMCPYA
                     RFQSAMFDKDTLVITYDQHRGEPRGARKRGVDHKARGLGDCVDCTMCVQVCPTGIDIR
                     KGLQYECIACAACIDACDEVMDKVGYPRGLIRYDTQRGIEGQRRHVLRPRTIVYGTLL
                     GLLVVGFGVSLMQRNVVGVDVLRDRNALFRERADGLIENVYNVKILNKDGDPHAFEIT
                     ATGLEGLQVDYGQDTVAVGPGEVRSVPVRIRVPRGELRGGADIQVTVRTTDARALEAA
                     GKARFLAPTH"
     gene            complement(2852..3142)
                     /locus_tag="VD737_04565"
     CDS             complement(2852..3142)
                     /locus_tag="VD737_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09864.1"
                     /translation="MRWPRLGQDVAIVLWPAFLVASVATMVCFAFIDPVLIGNDDYPP
                     PAFATRMTGYAVGFFFFWLVASLSSLITLYLVRTAHPEPTDAVADEGTRSPE"
     gene            complement(3175..4080)
                     /gene="ccoP"
                     /locus_tag="VD737_04570"
     CDS             complement(3175..4080)
                     /gene="ccoP"
                     /locus_tag="VD737_04570"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HXW09865.1"
                     /translation="MSTGWSLWIVILTVANILACVWLLRWTSKPKAADEKIGGGADTG
                     HVWDEDLREYNNPLPKWWLWLFYVTVVFGLLYLVLYPGLGNFAGLKSWTQEAQYEQEK
                     AAVEARASELLAPLAALPVGDLVDNEQAITTAHNLFQQNCAQCHGSDGGGARGFPNLA
                     NADWQWGGDADAVVATIANGRTAAMPPWGAVLGDDGVAEVVAYVQQLSGQPSDAAQAT
                     AGETRYQQVCAACHGLDGKGMAVLGAPNLTDDVWIYGGDAATLRQTIVAGRTGQMPAW
                     NDKLGEQRVKLLAAYVTRLARDGSR"
     gene            complement(4077..4247)
                     /locus_tag="VD737_04575"
     CDS             complement(4077..4247)
                     /locus_tag="VD737_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HXW09866.1"
                     /translation="MDIGTVRGLVTLALLIAFVALVVYVYGKRRKADFDEMARMPLED
                     DPPAEDRGSKTP"
     gene            complement(4251..4859)
                     /gene="ccoO"
                     /locus_tag="VD737_04580"
     CDS             complement(4251..4859)
                     /gene="ccoO"
                     /locus_tag="VD737_04580"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HXW09867.1"
                     /translation="MNHETVEKKVGLMGVLIALVISVGGLVEIVPLYFQQAVTEPAPG
                     VKPYPALQLEGRDVYIREGCYVCHSQMIRPFRSETERYGPYSRPGESVYDHPFQFGSK
                     RTGPDLARVGGRYSDDWHRVHLINPRDVVPESNMPRFPWLLEAGIDPVQTPKKMVALR
                     KVGVPYTDEDIASASAAVEGKTEMDALIAYLQGLGISSGAGT"
     gene            complement(4871..6292)
                     /gene="ccoN"
                     /locus_tag="VD737_04585"
     CDS             complement(4871..6292)
                     /gene="ccoN"
                     /locus_tag="VD737_04585"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HXW09868.1"
                     /translation="MAASESTYNYTVVRQFALMTIVWGLVGMAVGVLIAAQLLWPALN
                     FDVPWLSYGRLRPLHTNAVIFAFGGSALFATSYYVVQRTCHARLAFERLAAFTFWGWQ
                     AVIVLAAITLPLGITTSKEYAELEWPIDLLITVVWVAYAVVFFGTIALRKVKHIYVAN
                     WFFASYIITIAVLHIFNSLAIPVSATKSYSVYPGVVDAMVQWWYGHNAVGFFLTAAFL
                     GMMYYFIPKQAGRPVYSYRLSVVHFWALISTYMWAGPHHLHYTTLPDWAQSLGMVFSL
                     ILLAPSWGGMINGIMTLSGAWHKLRTDPILKFMIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTIGHVHSGALGWVAMISVGTMYALIPKLYGRTEMYSTKAIDVHFWTTTIGVV
                     LYITSMWIAGVMQGLMWRATNADGTLTYAFIESIKSTYPYYAVRLAGGTLVLAGMFLM
                     AWNVWKTIATRTSTADAPVVQPA"
     gene            6475..>6688
                     /locus_tag="VD737_04590"
     CDS             6475..>6688
                     /locus_tag="VD737_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004748395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09869.1"
                     /translation="MDTKVKDASPGKCPFSHIAGVGRTNRDWWPNQLRLELLHQHSAK
                     SDPMGKGFDYAKEFKGLDYKALKKDLV"
BASE COUNT         1099 a         2301 c         2288 g         1000 t
ORIGIN      
        1 accgcgccag cctggggcgc tcggcctgcg cgcgttccag cagcgcgacg atcccggaca
       61 acacggtggc gccgccgacg gcggtcacct cgatctcgag cggcggcccg aggttctgcg
      121 tcccggcgtg cacggccgag ccgcgggtct tggcgatggc ggtcgattcg cccgtgagca
      181 gcgattcgtc gatgcgcccg tcgacgtgct cggcgacgac gccgtccgcg gccaggatct
      241 cgccggtgcg cacgagcagg tggtcgcccg gccgcagctc gagcgcctgc acgtcctcga
      301 cctggccgcc gcgcacgcgt cgcgcggtga cgggcgcgag ccgggcgagg gcgtcggcga
      361 cgctgccggc gcggtgccgc gcgaccatct caacgtagcg cccgagcgcc aggaagaata
      421 cgaacatcgt gaccgagtcg aagtagacct cgccccgtcc gctcagcgcg ttccagacgc
      481 tcgccgtgaa ggccgcgacg atgccgagcg tgaccggcac gtccatgctg acggtgcgcg
      541 ccctgagcgc gtgccacgca ccgacgtaga acggccagcc ggcgtagatc gcgaccggca
      601 ccgtgatcag catgctgacg agccggaaga actcgtgcat gccgcgctcc acgccgccca
      661 cgtagagcgg cagcgcgaac atcatcacct gcatcatgcc gaagcccgcg acggccaggc
      721 gcttcagcgc cgtgcgacgc tcgagcagcg cgagctgctc ggccggctcg ccggcgagtg
      781 ggtgcgggcg cagaccgacg tgctcgagca cgcgcagcag gtctccgagc ctgacgcggg
      841 tcgggtccca caccaggcgc gcgcgggccg tggccgcgtt gacgctcacg tcgagcacgc
      901 cggcctgctg gtgcagtgcc ctgtcggcga gccagctgca cgcggagcag cgcaggccct
      961 cgagcagcac ggtgatggaa cgcgcgccgt ccggctcggc ccgcgtcagc cgttcgacga
     1021 gttccgggcg gtcgtaggcg gaccagcgat cggcacgcgg cggctcgtcg gcgcgtgcgc
     1081 cggcctggtc acggtagcgg tagtagtcgt cgaggccggc accggtgatg aactccgcga
     1141 cggccttgca gccgtgacag cagaccggct gctcgtgacc cttgacgcgc gcatggatgc
     1201 gcacgcccgc cgggatgggc tcgccgcagt ggaagcaagc agagcccggc gacggcgtca
     1261 tggagcccgc ggggcgtcct cgccgcagac ggtgatggcg ccgtcacgct ggcagtcggt
     1321 gcgcacgagc acgtccgggc gcgagatcgc caggtacagc gtgatcgcgc cgccgatcac
     1381 ggcgcccgcc ggcgggatga tcagcagcca gggccaggcc tggcggtacc acggtcgtgc
     1441 ggcgtcgatg ctcattgtgg tgccctgcgt gttcagtggg tcggtgcgag gaaacgtgcc
     1501 ttgccggccg cttcgagcgc gcgcgcatcg gtcgtgcgga cggtgacttg gatgtcggcg
     1561 ccaccgcgca gttccccgcg cggcacgcgg atgcgcacgg gcacgctgcg gacctcgccg
     1621 ggaccgacgg cgaccgtgtc ctggccatag tcgacctgca ggccctcgag accggtggcc
     1681 gtgatctcga acgcgtgcgg gtcgccgtcc ttgttcagga tcttgacgtt gtagacgttc
     1741 tcgatcagcc cgtccgcgcg ctcgcgaaag agcgcgttgc ggtcgcgcag cacgtcgacg
     1801 ccgacgacgt tgcgttgcat cagcgacacg ccgaagccga cgaccagcag tccgagcagc
     1861 gtgccataca cgatcgtccg cggacgcagc acgtggcgac gttgtccctc gatgccacgc
     1921 tgcgtgtcgt agcggatcag gccgcgcggg tagcctacct tgtccatgac ctcgtcgcag
     1981 gcgtcgatgc acgccgcgca ggcgatgcac tcgtactgca ggcccttgcg gatgtcgatg
     2041 ccggtcgggc acacctgcac gcacatcgtg cagtcgacgc agtcgccgag cccgcgcgcc
     2101 ttgtggtcga caccgcgctt gcgcgcgccg cgcggctcgc cgcggtgctg gtcataggtg
     2161 atgaccagcg tgtccttgtc gaacatcgcg ctctggaagc gcgcgtaggg gcacatgtac
     2221 ttgcagacct gctcgcgcat gtggcccgcg ttgacgtacg tcgcgccgcc gtagaacagc
     2281 acccagaagg tttcccacgg gccggtcgag aagcccacga gcttgcccgc gagctcggtg
     2341 atcggcgtga agtagccgac gaacgtgaac ccggtccaca gcgcgaaggc gatccacagg
     2401 aactgcttgg ccgccttgcg caggactttg tcccgcgtcc acggcgcctt cgcgagtttg
     2461 atctgctggt tgcggctgcc ttcgacccag cgctccatcc agatgaacac ttcggtccac
     2521 accgtctgcg ggcaggcgta gccacagaac agccggccgg ccagcgccgt gaagaagaac
     2581 agcgccagcg ccgcgatcac cagcagccag gtcaggaagt agaagtcctg cggccagagc
     2641 gtcaggccga agatgtggaa ctggcgcgcc ggcaggtcga acagcaccgc ctgccgcccc
     2701 tgccacggga cccacggcag caggtagtag aggccgagca gtccccacac cgcgtacacg
     2761 cgcaggcgct ggaaccgccc ctgtatttcg cggggataga tcttctggcg cgccgcgtac
     2821 agcgcgcctt cgacccgctc ttcgacttcg ctcattcggg actccgtgtc ccttcgtcag
     2881 ccaccgcgtc cgtcggttcg ggatgcgcgg tgcgcaccag gtacagcgtg atcaggctcg
     2941 acagcgacgc cacgagccag aagaagaaga agcccaccgc gtagcccgtc atgcgcgtcg
     3001 cgaacgcggg cggcggatag tcgtcgttgc cgatcagcac ggggtcgatg aaggcgaaac
     3061 acaccatcgt cgcgacgctc gcgaccagga acgcgggcca gagcacgatg gccacgtcct
     3121 gcccgaggcg cggccaccgc atccgacccg tgtattcggc ctgggcctcc cacactagcg
     3181 tgagccgtcc cttgccagcc gcgtgacgta ggcggccagc agcttgacgc gctgctcgcc
     3241 gagcttgtcg ttccaggccg gcatctggcc cgtgcgcccc gcgacgatcg tctggcgcag
     3301 cgtcgccgcg tcgccgccgt agatccagac gtcgtccgtc aggttcggcg cgccgagcac
     3361 ggccatgccc ttgccgtcga gcccgtggca ggccgcgcag acctgctggt agcgcgtctc
     3421 gccggcggtc gcctgcgccg cgtcgctggg ctggccgctc agctgctgca cgtaggcgac
     3481 cacttcggcg acgccgtcgt cgccgagcac ggcgccccag ggcggcatgg ccgcggtgcg
     3541 cccgttggcg atcgtcgcga cgaccgcgtc ggcgtcaccg ccccactgcc agtcggcgtt
     3601 tgccaggttc gggaagccgc gcgcgccgcc gccgtccgag ccgtggcact gcgcgcagtt
     3661 ctgctggaac aggttgtgcg cggtcgtgat cgcctgctcg ttgtcgacca ggtcgccgac
     3721 cggcagcgcc gcgagcggcg caagcagttc gctggcgcgt gcctcgacgg ccgccttctc
     3781 ctgctcgtac tgcgcctcct gcgtccagct cttcagtccc gcgaagttgc ccaggccggg
     3841 atagagcacc aggtacagca ggccgaacac gaccgtcacg tagaacagcc acagccacca
     3901 cttgggcagc ggattgttgt actcgcgcag gtcttcgtcc cagacgtggc cggtgtcggc
     3961 gccgcccccg atcttctcgt cggcggcctt gggcttcgac gtccagcgca gcagccagac
     4021 gcacgcgagg atgttggcga ccgtcaggat gacgatccag aggctccagc cagtactcat
     4081 ggggtcttgc tccctcgatc ttcagcgggc gggtcgtctt cgagcggcat gcgagccatc
     4141 tcgtcgaagt ccgccttgcg tcgtttgccg tagacgtaca cgaccagcgc cacgaacgcg
     4201 atcagcagcg cgagcgtgac caggccgcgg acggtgccga tgtccatgcc tcaggtcccc
     4261 gcgccggagc tgatgccgag gccctgcagg tacgcgatca gcgcgtccat ctcggtcttg
     4321 ccttcgaccg cggcggacgc actggcaatg tcctcgtccg tgtacgggac gccgaccttg
     4381 cgcagcgcca ccatcttctt cggcgtctgc acggggtcga tgcccgcctc gagcagccac
     4441 gggaagcgcg gcatgttgga ctccggcacg acgtcgcgcg ggttgatcag gtgcacgcgg
     4501 tgccagtcgt ccgagtagcg gccaccgacg cgcgcgaggt cgggccccgt gcgcttggag
     4561 ccgaactgga acgggtggtc gtacaccgac tcgcccggcc tcgagtacgg cccgtagcgc
     4621 tcggtctcgg agcggaaggg ccggatcatc tgcgagtggc agacgtagca gccctcgcgg
     4681 atgtagacgt cgcggccctc cagctgcagt gccgggtacg gcttcacacc gggcgccggc
     4741 tcggtcaccg cctgctggaa gtacagcggc acgatctcga ccaggccgcc gacgctgatc
     4801 accagcgcga tcagcacgcc catgaggccg actttcttct cgactgtttc gtggttcatg
     4861 cgtggatacc tcaggcgggc tgcacgaccg gcgcgtccgc ggtgctggtg cgcgtcgcga
     4921 tcgtcttcca gacgttccag gccatcagga acatgcccgc gagcaccagc gtgccgcccg
     4981 cgaggcggac cgcgtagtac gggtacgtcg acttgatcga ctcgatgaac gcgtaggtga
     5041 gcgtgccgtc ggcgttcgtc gcgcgccaca tcaggccctg catgacgccg gcgatccaca
     5101 tcgaggtgat gtagagcacc acgccgatcg tcgtcgtcca gaagtgcacg tcgattgcct
     5161 tcgtgctgta catctccgtg cggccataca gcttcgggat cagcgcgtac atcgtgccga
     5221 ccgagatcat cgcgacccag ccgagggctc cggagtgcac gtggccgatc gtccagtcgg
     5281 tgtagtgcga cagcgcgttg accgtcttga tcgacatcat cggcccctcg aaggtcgaca
     5341 tgccgtagaa cgacaacgag acgatcatga acttgaggat cgggtcggtg cgcagcttat
     5401 gccaggcgcc cgacagggtc atgatgccgt tgatcatgcc gccccacgac ggcgcgagca
     5461 ggatcagcga gaacaccatg ccgagcgact gcgcccagtc gggcagcgtc gtgtagtgca
     5521 ggtggtgcgg gcccgcccac atgtacgtgg agatcagcgc ccagaagtgc acgaccgaga
     5581 gccggtacga gtagaccggg cggccggcct gcttcgggat gaagtagtac atcatgccga
     5641 ggaaggccgc ggtcaggaag aaaccgacgg cgttgtgccc gtaccaccac tgcaccatcg
     5701 catcgacgac gcctgggtag acgctgtacg acttggtggc cgacaccggg atcgccaggc
     5761 tgttgaagat gtgcagcacc gcgatcgtga tgatgtagga cgcgaagaac cagttcgcga
     5821 cgtagatgtg cttgaccttg cgcagcgcga tcgtgccgaa gaagacgacc gcgtaggcca
     5881 cccacacgac cgtgatcagc aggtcgatcg gccactcgag ctcggcgtac tccttggagg
     5941 tcgtgatgcc gagcggcagc gtgatcgcgg cgagcacgat gaccgcctgc cagccccaga
     6001 acgtgaatgc ggcgagccgc tcgaacgcga gccgcgcgtg gcaggtgcgc tgcacgacgt
     6061 agtacgaggt cgcgaacagc gccgagccgc cgaacgcgaa gatcaccgcg ttggtgtgga
     6121 gcggccgcag ccgcccgtag gagagccacg ggacgtcgaa gttgagcgcc ggccacagga
     6181 gctgcgccgc gatcagcacg ccgaccgcca tgccgaccag cccccagacg atcgtcatga
     6241 gcgcgaactg gcgcacgacc gtgtagttgt aggtggattc cgacgcggcc atcgagcctc
     6301 ccggggcgag tgcagcctgc gatgctaggg acgcgggcgc ggcggctttt gacctgagtc
     6361 aaacggaccg tgcgatagcc ggcaacaatc gaccggatcg caacaatcaa cttcacaacg
     6421 gccgcggcac cggctgcaat agcgtccgat cagacgcgct caccggagac agccatggac
     6481 accaaggtca aggacgcgag cccgggcaag tgcccgttca gccacatcgc cggcgtcggc
     6541 cggaccaacc gcgactggtg gcccaaccag ctgcgcctcg agctgctgca ccagcattcg
     6601 gcgaagtccg atccgatggg caagggcttc gactacgcga aggagttcaa gggcctcgac
     6661 tacaaggcgc tcaagaagga cctggtca
//
LOCUS       DASPFR010000180         6152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1518220, whole genome shotgun sequence.
ACCESSION   DASPFR010000180 DASPFR010000000
VERSION     DASPFR010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518220"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1369
                     /locus_tag="VD737_04595"
     CDS             191..1369
                     /locus_tag="VD737_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009485198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HXW09870.1"
                     /translation="MGPPIESKLPAVGTTIFTVMSRLAAEHEAINLSQGFPDFEPPPR
                     LVELVEQHMRRGPRAQNQYAPMTGLPVLREAIAAKVAEHYRRHVDPEAAITVTSGGTE
                     ALFCAIQALVQRGDEVVLLDPCYDSYEPSVTLAGGRAVHVPLMRRPTRDGYCGIDWQR
                     LREALTDRTRLLVVNFPHNPSGAVLDRTDLDSLAEVLRGTPTWVLADEVYEHMVFDGR
                     THQSLLTHDELATRSLVVSSFGKTFHATGWKVGYCVAPPELTTEFRKVHQFVQFAVAT
                     PLQAGIAGYLQECPGHAHELPAFYQAKRDHFARLLHETRFRFTPAASTYFQLVDYSAI
                     SDLPDTDFARELTVRHGVAAIPVSVFSASPAADERLVRFCFAKHEATLDAAAAKLRNL
                     "
     gene            1371..2168
                     /locus_tag="VD737_04600"
     CDS             1371..2168
                     /locus_tag="VD737_04600"
                     /inference="COORDINATES: protein motif:HMM:NF007757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXW09871.1"
                     /translation="MTANLRVTMIQTELAWHAPATNRRTLARHFRGLVGHTDLIVLPE
                     MFTTGFTMDAAGLAETMEGETVGWLREEAAAVGCAITGSLIVREGSYRHYNRLLFVTP
                     DGEVRHYDKRHLFRMANEQSHYAAGKHRLIVTWKGWRICPLICYDLRFPVWSRNRGDY
                     DVLLYVANWPAKRRVAWSALLRARAIENLCYVVGVNRIGKDGNGASYGGDSAAVDFQG
                     LALGGDHGGGDFVETVVLDREALQKYREKFPAHLDADDFELKSGSTA"
     gene            2165..3436
                     /locus_tag="VD737_04605"
     CDS             2165..3436
                     /locus_tag="VD737_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXW09872.1"
                     /translation="MTGSDRDGAGDAPEHRAPAPPDADASPPRPESLSPRTLVTHPPE
                     VTLPPGNRPLVAPIYQSVKFTFDDVGETLEHFQRRRGGFFYSRVSNPTLRQLEGVLAR
                     LQGRDGCLLTGSGIAAIAVPLIALCRAGDHVVYFAEQYQPTQKLVGRLLKRYGVAGTM
                     LSITDLEALERVLASTPTRVVVFESPTNPVLKVADLERITELARRHGALTLLDNTLAG
                     FHNHGQHAVDLYSHSLTKYASGHGDVMGGAIIGEPKLIDSLRDDANMLGATLDPHAAF
                     LVLRGMKTYFLRWEAQCRTAQRVAEFLARHPGVERVRYPGLSDDPGHALARRQMRDFG
                     TLVTFDIAGGEAAGTRFAEALNLFAIAASLGSTESLVMPPALQQPRGLTAEQRRWSDI
                     GPGTVRLSLGLEDADDLLADLSAALVKASPD"
     gene            3508..5874
                     /locus_tag="VD737_04610"
     CDS             3508..5874
                     /locus_tag="VD737_04610"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXW09873.1"
                     /translation="MDAASLTRDGFRVGEWLIEPGASRATDASGRVVELTHNQTRLLV
                     ALALRHGEPVARRELRDSLWPGAGGTEEKLRETVAGLRTLFAESARHPRYIASVGQDA
                     YALLAHVEPLPPPAPAPHTALPAGRLHWVLAELRRRNVVKVAVSYLIGMWIVLQVAEV
                     TFAPLHFPNWWMTALTIMAIIGLPIVAVLAWAYEITPQGIVIDPSSSGAAAVKMPRAR
                     QSLAPAIVAGVAAMAAVTGLAWWRSIDTPLSPAEATEPTVESGPPSVAVLPLVDLTPK
                     GDTAWLGDGLSEELSTRLAQVPGLRVAARTSAFEFRGRNLDVRKIGQLLGVRHVLEGS
                     VRRDGDAVRVTVQLIDARTGFHVWAGNFDRAWRDVLSLQDEIARSVTAALRVVLMDGP
                     VPGPASAGANLLDSRGIDPYLEGLARLRQPGDRSSLDEAEQAFRTAIRIAPGFAGAHA
                     GLCRTLARGFDYSRDRRVLAEAEQACQRSLALDATLIDTEKALAGLYVSDGRFRDAAD
                     AYSKVIARNARDADALLGLGQAYEGLGRGREAEASLRKATEIEPAYWSTYMALGRFLF
                     QRGRADEALAAFRRVTELVPSSAIAWSNVGAALQLKGDLATAEQAYVTSLKLEPSKGA
                     YSNVATIQFALGRFEDAAGSFARAVELGPHDQVVHGNLADALWQIDGRREEATRAYGR
                     AIELAERELAATPNDSVLRAQVGYYYGRVGDGQRSLAYLDEALRAGPELLYVQYYRGV
                     AAADRGDRETALAAVSDMIKLGYPREQLRSAPEFRSLSEDPEYKKLVG"
     gene            5936..>6152
                     /locus_tag="VD737_04615"
     CDS             5936..>6152
                     /locus_tag="VD737_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09874.1"
                     /translation="MDDGTVYCTPDKGNVWAKNPDPKQPETVEWRRGPNVTNFRLRFH
                     RQAIQGNGATSDWPFEGAPPEPPNGTGI"
BASE COUNT          969 a         2141 c         2105 g          937 t
ORIGIN      
        1 gggagcgtca cgagccaggg agggcgagtg cgagccggca gggatgcctc ttactggcgt
       61 ctcccccgac caaaccccgg acgccggcgg cgacgcgccg gtactgcgct ccgaaagcca
      121 cgtcggacgt ttgcacgacc gtcgcgctta acgaaatgcc cgatccggcc gcgctgctag
      181 aatcaaacgg atgggcccgc cgatcgagag caagttgccg gccgtcggca cgacgatctt
      241 caccgtcatg tcgcggctgg cggccgaaca cgaggccatc aacctctcgc agggcttccc
      301 tgatttcgag ccgccgcccc ggctcgtcga actcgtcgag cagcacatgc gccgcgggcc
      361 gcgcgcgcag aaccagtacg cgccgatgac cgggctgccc gtgctgcggg aggcgatcgc
      421 ggcgaaggtg gccgaacact acaggcgtca cgtcgatccc gaagccgcga tcaccgtgac
      481 ctccggtggc accgaggcgc tgttctgcgc gatccaggcg ctggtgcaac gcggcgacga
      541 agtcgtgctg ctcgacccgt gctatgactc gtacgagccc tcggtcacgc tggccggagg
      601 acgggcggtc cacgtgccgc tcatgcggcg accgacccgc gacggctatt gcggcatcga
      661 ctggcagcgg ctgcgcgagg cgttgaccga ccgcacccgc ctgctcgtcg tcaattttcc
      721 gcacaacccc agcggtgccg tgctcgaccg caccgacctg gactcgctcg ccgaggtgct
      781 gcgcggcacg ccgacctggg tccttgcaga cgaggtctac gagcacatgg tcttcgacgg
      841 acggacgcac cagagcctgc tcacgcacga cgagctcgcc acgcgcagcc tggtcgtctc
      901 gtcgttcggc aagacctttc acgcgaccgg ctggaaggtc ggctactgcg tcgcgccgcc
      961 ggagttgacg accgagttcc gcaaggtgca ccagttcgtg cagttcgcgg tggccacgcc
     1021 gctgcaggcg ggcattgccg gctacctgca ggaatgcccg gggcacgcgc acgaactgcc
     1081 ggcgttctac caggcgaagc gggaccactt cgcccggctc ctgcacgaga cacgctttcg
     1141 cttcacgccc gccgccagca cgtacttcca gctggtcgac tattcggcga tcagcgacct
     1201 gcccgacacc gatttcgccc gcgagctgac cgtgcgccac ggcgtcgccg ccatcccggt
     1261 ctcggtgttc tcggcgtcgc cggccgcgga cgagcgactc gtccgattct gctttgccaa
     1321 acacgaggcc acgctcgacg cggcggccgc caagctgagg aacttatgaa atgactgcga
     1381 acctcagagt cacgatgatc cagaccgagc tcgcctggca cgccccggcg accaatcgcc
     1441 gcacgctggc acggcacttc cggggcctcg tcgggcacac cgacctgatc gtgctgccgg
     1501 agatgttcac gacgggcttc acgatggacg cggcgggcct cgcagagacg atggagggcg
     1561 agacggtcgg atggctgcgg gaggaggcgg cggcggtcgg ctgtgccatc accggcagcc
     1621 tgatcgtgcg cgagggttcg tatcgccact acaaccggtt gctgttcgtg acgcccgacg
     1681 gcgaggtccg gcactacgac aagcgccacc tgttccggat ggccaacgag cagtcgcatt
     1741 acgcggccgg caagcaccga ctcatcgtca cgtggaaggg ctggaggatc tgcccgctca
     1801 tctgctacga cctgcgcttt ccggtctgga gtcgcaaccg aggcgattac gacgtgctgc
     1861 tgtacgtggc caactggccg gccaagcggc gggtcgcctg gtcggccctg ctgcgggcgc
     1921 gcgcgatcga gaacctgtgc tacgtcgtgg gcgtcaaccg catcggcaag gacggcaacg
     1981 gcgcctccta cggcggtgac agcgccgccg tcgatttcca gggcctggcg ctcggtggcg
     2041 accacggcgg cggcgacttc gtcgagaccg tcgtgctcga tcgcgaggcg ctgcagaaat
     2101 accgcgagaa gttcccggcg cacctcgacg cagacgactt cgaactcaag tcaggctcga
     2161 cggcatgacc ggcagcgatc gcgacggcgc cggcgacgca ccggagcacc gcgcgcctgc
     2221 gccgccggac gccgacgcat cgccgccccg cccggagtcc ttgtcgccac ggaccctggt
     2281 cactcacccg ccggaggtca cgctgccgcc cggcaacagg ccgctcgtcg cgccgatcta
     2341 ccagtccgtc aagttcacgt tcgacgacgt cggcgaaacg ctcgagcatt tccagcgcag
     2401 gcgcggtggt ttcttctact cgcgggtttc gaaccccacg ctgcgccagc tggagggcgt
     2461 gctggcccgc ctgcagggac gcgacggctg cctgttgacg ggctccggca tcgcggcgat
     2521 cgcggtcccg ctgattgcgc tttgcagagc cggcgaccac gtcgtgtact tcgccgagca
     2581 gtaccagccg acccagaaac tcgtgggccg cctgctcaag cggtacggcg tcgcaggcac
     2641 catgctttcg atcacggacc tcgaagcgct cgagcgcgtg ctggcgagca ccccgacccg
     2701 cgtcgtcgtc ttcgagtcac cgaccaaccc cgtgctcaag gtcgccgacc tcgagcgcat
     2761 cacggagctc gcgcgacggc acggcgcact cacgctgctc gacaacacgc tggccggctt
     2821 ccacaaccac ggccagcacg ccgtcgacct gtattcgcac agcctgacca agtacgcgtc
     2881 gggacacggc gacgtgatgg gcggggcgat catcggtgaa ccgaagctga tcgattcgct
     2941 gcgcgacgac gccaacatgc tcggcgcgac gctcgatccg cacgcggcgt tcctggtgct
     3001 gcgcggcatg aagacctatt tcctgcgctg ggaagcgcag tgccgcaccg cgcagcgcgt
     3061 cgccgagttt ctcgcgcggc atccgggcgt ggagcgcgtg cgctatcccg gcctgtccga
     3121 cgatcccggt cacgcgctcg cgcgccggca gatgcgcgat ttcggcaccc tcgtgacgtt
     3181 cgacatcgcg ggtggcgagg cggcgggcac gcggttcgcc gaggcgctga acctgttcgc
     3241 gatcgccgcc agcctgggtt cgaccgagtc gcttgtgatg ccgccggcac tccagcagcc
     3301 gcgcgggctg actgcggagc agcggcgctg gagcgacatc ggtccgggca ccgtgcgcct
     3361 ctcgctcggc ctcgaggacg ctgacgatct tctcgccgac ctgtccgccg cgctggtgaa
     3421 ggcaagtccc gactgacctg tggcctcggt gctggctctg ctatgcttct cctcgctcga
     3481 cacgagggag tcgatgagtc gagggtcatg gacgccgcca gcctcactag ggatggcttc
     3541 cgggtgggcg agtggctcat cgagcccggt gcttcgaggg ccacggacgc gtccggccgt
     3601 gtcgtcgagc tgacacataa tcaaacccgg ctgctcgtcg cattggcact gcgccacggc
     3661 gagcccgtcg cgaggcgaga gttgcgcgac tcgctctggc cgggcgccgg tggcaccgag
     3721 gagaagttgc gcgaaaccgt cgccggcctg cgcaccctct tcgccgagag cgctcgccac
     3781 ccacgctaca ttgcctcggt cggccaggat gcgtacgcgc tgctggcgca cgtcgagccg
     3841 ctcccgcctc ctgcacccgc gccccacacg gcactgcctg caggacgcct gcactgggtg
     3901 ctggcggaac tgcgccggcg caacgtcgtg aaggtggccg tgtcgtacct gatcggcatg
     3961 tggatcgtgc tgcaggtcgc cgaggtgacg tttgcgccgc tgcattttcc gaactggtgg
     4021 atgacggcgc tcaccatcat ggcgatcatc ggcctgccga tcgtggcggt gctcgcgtgg
     4081 gcgtacgaga tcacgccgca gggcatcgtc atcgacccgt cgagcagcgg cgccgcggcc
     4141 gtgaagatgc cgcgcgcgcg gcaatccctc gcgcctgcga tcgtcgccgg cgtcgccgcc
     4201 atggcggcgg tgaccggttt ggcgtggtgg cgatcgatcg atacaccgtt gtcgcctgcg
     4261 gaagccaccg aacccacggt ggagtcgggt ccaccctccg tcgcggtcct gccgctcgtc
     4321 gacctgaccc cgaaaggcga caccgcgtgg ctcggcgacg gcctgagcga agagctgtcg
     4381 acgcgcctcg cccaggttcc ggggctgcgc gtcgcggccc gcacctcggc gttcgagttt
     4441 cgcggcagga acctcgacgt ccgcaagatc ggccagttgc tgggtgtgcg ccacgtcctg
     4501 gagggcagtg tccgccggga tggagacgcc gtccgcgtca cggtgcagct gatcgacgca
     4561 cgcaccgggt ttcacgtctg ggccggcaac ttcgaccggg catggcggga cgtcctctcg
     4621 ctgcaggacg agatcgcgcg ttcggtgacg gcggcgctgc gcgtcgtgct catggatggc
     4681 ccggttcccg gcccggccag cgctggcgcc aacctgctcg attcacgggg catcgatccg
     4741 tacctggaag ggcttgcgcg gctgcggcaa cccggcgacc ggagctcgct ggacgaggcc
     4801 gaacaggcgt tccgcaccgc gatccggatc gcgccggggt tcgcgggcgc ccatgcgggg
     4861 ctctgccgca cgctggcgcg cggcttcgac tacagccgcg accggcgagt gctcgcggag
     4921 gccgaacagg cgtgccagcg ttcgctcgcg ctcgacgcca cgctgatcga caccgaaaag
     4981 gcgctcgcgg gcctgtacgt ctccgacggc cggttccgcg acgcggccga cgcgtattcg
     5041 aaggtcatcg cgcgcaacgc gcgggatgcg gatgcgctgc tgggcctggg acaggcatac
     5101 gaagggctcg ggcgcggacg cgaagccgag gccagcctgc gcaaggccac cgaaatcgaa
     5161 ccggcttact ggagcaccta catggcgctc ggccgcttcc tgttccagcg gggtcgcgcc
     5221 gacgaggcac tggccgcgtt ccggagggtc accgaactcg taccgtcgag cgccatcgcc
     5281 tggagcaacg tcggcgccgc gctgcagctc aagggcgacc tggcgaccgc ggagcaggcg
     5341 tacgtcacgt ccctgaagct ggagccgtcc aagggcgctt actcgaacgt cgccaccatc
     5401 cagttcgcgc tcgggcggtt cgaggacgcg gcggggagct tcgcgagggc ggtcgaactc
     5461 ggaccgcacg accaggtcgt gcacggcaac ctcgcggacg cactgtggca gatcgacggt
     5521 cggcgggagg aggcaacccg ggcgtacggt cgagcgatcg aactggcgga acgcgaactt
     5581 gcggcgacgc ccaacgattc ggtcctgcgc gcgcaggtcg gctactacta tggccgggtg
     5641 ggcgatggcc agcgctcgct cgcctacctc gacgaggcgc tgcgggccgg tccggaactg
     5701 ctgtatgtcc agtactaccg aggcgtcgcc gcggcggacc gcggcgaccg cgaaactgcg
     5761 ctggcggcag tgtcggacat gatcaagctg ggatacccgc gcgaacagtt gcgttccgcg
     5821 ccggagtttc gcagtctgtc ggaggacccg gaatacaaga aactcgtcgg ataagtcacg
     5881 gcaagggtcg cgtcgcgagg gaggatccac tatggccagg ccaatacgaa tcttgttgga
     5941 cgacggtacg gtttactgca ctcccgacaa gggcaacgtc tgggcgaaga accccgatcc
     6001 caagcaaccc gaaaccgtcg aatggaggcg agggcccaac gtcacgaatt tccgcttgcg
     6061 gtttcacagg caggccatcc agggcaacgg tgcgacgtcg gactggccgt tcgagggcgc
     6121 gccaccggag ccgcccaatg ggaccggcat cg
//
LOCUS       DASPFR010000181         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1761365, whole genome shotgun sequence.
ACCESSION   DASPFR010000181 DASPFR010000000
VERSION     DASPFR010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1761365"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VD737_04620"
     CDS             <1..472
                     /locus_tag="VD737_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HXW09875.1"
                     /translation="CSPADMTWRAGVDVVSFGATKNGAMNAEAVVVFDEAVAATLPYL
                     MKRGGQVLSKARYVSAQLERYLADDRWLQRARAANGHARSLADRLLAAGIELVGEVQV
                     NMLFTRMPADVVAALDRGPFRYYKLGLDQRFVCRHDQEPEGMDALVQTVTRALA"
     gene            469..1329
                     /locus_tag="VD737_04625"
     CDS             469..1329
                     /locus_tag="VD737_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HXW09876.1"
                     /translation="MSESARARRRAWLALTLVVLVAGSASRVHAESLEAIPLQYRLAE
                     ELLPILQPLLPADAALTGAGDVLLLRADEATVQQVRAAIATLDRPPRQLLITVAQATT
                     GTDAGAGVRGSATIEAGDVQVGVNRPPAPQSGARVTVHGGREQVELHEVSSVRALEGR
                     ETYVALTTSRPYTSTSTVHDGPHHVTEVHTLGRQDAQTGFVATPRLHGDRVTLEISPS
                     QQGFASSRRDANVTTRSLVTTVTGRLGEWIELGGVTSTGTDTEQGLIVWGARSELTRY
                     SAWVKVEEDR"
     gene            1392..2321
                     /locus_tag="VD737_04630"
     CDS             1392..2321
                     /locus_tag="VD737_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HXW09877.1"
                     /translation="MDDRLARCRVAVVGAGRVGGELIRHLALIGVGHLEVFERDRHVA
                     ATMRHHHPVHEGDFWDCLTLDRLRTFDFAVGTIDDPEARTRLNQKCLVASVNLLQVWT
                     EDDVATVGAYPFGSLDDCACYECATDRTAVPMPIASLKLTVEGTRSYTPAGAEVTTAG
                     IAGALAVALLARIAAGSHGRVARRASIEATSGAGRSIELQRDPDCARCGGLVRPVAIV
                     HTRNSWVVSPEVEQACPEMLDEYVQLSDAVEGLAGRSFKVGELAQRYPGGPIPVKFAL
                     TTLGGRAICLDFEDLAQGRPGGARSPAKVHPTG"
BASE COUNT          340 a          847 c          879 g          340 t
ORIGIN      
        1 ctgctcgccc gccgacatga cctggcgtgc cggcgtggac gtcgtctcgt tcggtgcgac
       61 caagaacggc gcgatgaacg ccgaggccgt ggtggtgttc gacgaggcgg tcgccgcaac
      121 gctgccgtac ctgatgaaac gtggcggcca ggtgctctcc aaggcccgct acgtctctgc
      181 ccagctcgaa cgctacctgg ccgacgatcg ctggctgcag cgcgcccgcg cggccaacgg
      241 gcacgcacgt tcgctggcgg accggttgct ggccgcgggc atcgagctcg tcggcgaggt
      301 gcaggtcaac atgctgttca cacgcatgcc ggcggacgtc gtggccgcgc tcgatcgagg
      361 ccccttccgc tattacaagc tcgggctcga ccagcgcttc gtctgccggc atgaccagga
      421 acccgagggc atggacgcgc tcgtccagac cgtgacgcgg gcactcgcat gagcgaatcc
      481 gcccgcgcgc gtcgccgtgc gtggctcgcg ctcacactcg tggtgctcgt ggctgggtcg
      541 gccagccggg tgcacgccga gtcgctcgag gcgattccgc tgcagtaccg cctggcggag
      601 gaactgctgc cgatcctgca gccgttgctg ccggccgacg ccgcgctgac cggcgcgggc
      661 gacgtgctgc tgctgcgcgc cgacgaggcc acggtgcagc aggtgcgggc cgcgatcgcg
      721 acgctcgacc gcccaccacg gcagttgctg atcaccgtgg cgcaggcgac gaccgggacg
      781 gacgccgggg ccggtgtgcg cggctccgcg acgatcgagg ccggcgacgt gcaggtcggc
      841 gtcaaccgcc cgccggcgcc gcagtccggc gcacgcgtga ccgtgcacgg gggtcgggaa
      901 caggtcgagc tgcacgaggt ctcgagcgta cgtgcgctcg agggccgcga gacctacgtc
      961 gcattgacga cgtcgcggcc ctacacgtcc acgtcgacgg tccacgatgg cccgcaccac
     1021 gtcaccgaag tgcacacgct gggccgccag gacgcgcaga ccgggttcgt ggcgacgccg
     1081 cgcctgcacg gcgaccgggt gacgctggag atttcgccat cccagcaagg atttgcgtct
     1141 tcgaggcgcg acgcgaacgt cacgacgcgg tcactggtca cgacggtcac gggccgcctc
     1201 ggcgaatgga tcgagctcgg cggcgtcacg tccaccggga cggacactga gcaggggctc
     1261 atcgtctggg gagcgcgctc ggaattgacg cggtactcgg catgggtcaa ggtcgaggaa
     1321 gaccgctgac ggcgcgctcc cacaggtccg cgcactccat catgacgccg actccaccga
     1381 acccggagcc catggacgac cgcctcgcac ggtgccgcgt cgcggtcgtc ggcgcggggc
     1441 gcgtcggggg cgagctgatc cgccacctgg cgctgatcgg cgtcggccac ctcgaagtct
     1501 tcgaacggga ccgccacgtc gccgcgacca tgcgtcacca tcatcccgtg cacgagggcg
     1561 acttctggga ctgcctcacg ctcgacaggc tgcggacgtt cgacttcgcg gtcggcacga
     1621 tcgacgaccc ggaggcgcgc acgcgcctga accagaagtg cctggtggcg agcgtcaacc
     1681 tcttgcaggt gtggaccgag gacgacgtcg cgacggtcgg tgcctatccg ttcggatcgc
     1741 tcgacgattg cgcgtgctac gaatgcgcga ccgaccgcac cgcggtgccg atgccgatcg
     1801 catcgctcaa gctcaccgtc gagggtacgc ggtcgtacac gcccgccggc gcggaggtca
     1861 cgacggccgg catcgccggg gcgctcgccg tggcgctgct cgcccgcatc gcggccggtt
     1921 cccacggccg ggtggcacgg cgtgcgtcga tcgaggcgac ctcgggggcc ggccgttcga
     1981 tcgaactgca acgcgacccc gactgtgcgc gttgcggcgg cctcgtgcga ccggtcgcaa
     2041 tcgtgcacac gcgcaactcc tgggtcgtgt cgccggaggt cgagcaggcg tgtcccgaga
     2101 tgctcgacga gtacgtgcag ctgagcgacg cggtcgaggg cctcgcgggg cggtccttca
     2161 aggtcggcga gctggcccag cgttatccgg gcggcccgat cccggtcaag ttcgcgctga
     2221 cgacgctggg cgggcgcgcg atctgcctcg atttcgagga tctcgcgcag gggcggcccg
     2281 gcggcgcgcg cagcccggcc aaggtccacc cgaccgggta agcgtcaggg cggcgcggcg
     2341 gccggcccgg cgccggtcgg cggtgcggcg gtggccggcg gcggaccgat cacctcgagg
     2401 ctcttc
//
LOCUS       DASPFR010000182         4674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_53474, whole genome shotgun sequence.
ACCESSION   DASPFR010000182 DASPFR010000000
VERSION     DASPFR010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53474"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1633)
                     /gene="lysS"
                     /locus_tag="VD737_04635"
     CDS             complement(116..1633)
                     /gene="lysS"
                     /locus_tag="VD737_04635"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HXW09878.1"
                     /translation="MSDAEQPSPAVTDVSADAENHLIAERKAKLERLRETGNAFPNDF
                     RRNAAADDLQTAYGAHAAEWFDAHMIRVAVAGRMMAKRIMGKASFARLTDRSGSIQLF
                     LQQSALGEVYDDFKGWDVGDIVGAEGTLFKTRTGELSVKVERLRLLVKSLRPLPDKWH
                     GLADVEQRYRQRYVDLITSEESREVFRVRTRIVQFLRHFLDSLDFLEVETPMLQVIPG
                     GAVAKPFVTHHNALDVDMYLRIAPELYLKRLVVGGFERVYEINRNFRNEGLSTRHNPE
                     FTMLELYQAYADYRDLMDLVERMFQGVADTICGTRRVEYQGETFDFGSTFRRVTIEDL
                     VVHFNPGIERALLRDVQYLREWCGKLAIPCKPDDGAGKLQVEIFEKTAEHRLAQPTFV
                     YAYPAEVSPLARRNDSDPFLTDRFEFFVGGREIANGFSELNDAEDQAERFRAQAARKS
                     GGDEEAMFYDADYVRALEYGLPPTAGLGIGIDRLVMLLTDRHTIREVVLFPAMRS"
     gene            complement(1764..2807)
                     /gene="prfB"
                     /locus_tag="VD737_04640"
     CDS             complement(1764..2807)
                     /gene="prfB"
                     /locus_tag="VD737_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HXW09879.1"
                     /translation="MRSGGTFDYEGKHERLTEVLRELEDPRIWQNPERAQELGRERTK
                     LDGVVATLDKLTHDLAEAGELLDLAEAESDEGTAAAVQQDLARVDREVKKLEFKRMFP
                     GEMDSHNAFLDIQAGAGGTEAQDWAQMLMRMYLRWGDKNGFKTEVIDLNDGEVAGIKS
                     ATIRFDGEYAYGWLRTETGVHRLVRKSPFDSNARRHTSFASVFLSPEIDDDIDIDINP
                     ADLETDVYRSSGAGGQHVNKTESAVRIRHLPSGIVVACQSERSQHKNRSTAMKMLKAK
                     LYELEVNKRNAAAKVLEDSKSDVSWGNQIRSYVLDQSRIKDLRTGIEIGNTTAVLDGD
                     LGPFLEESLKQGL"
     gene            complement(2893..>4674)
                     /gene="recJ"
                     /locus_tag="VD737_04645"
     CDS             complement(2893..>4674)
                     /gene="recJ"
                     /locus_tag="VD737_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HXW09880.1"
                     /translation="RPAGGPLMSTPARRIVRRDPRAELQAALPASLHPVLRRVYGARG
                     VTHADDLALRLDQLLPVRSLEGVEQAAERLVAAHRQRARVLVIGDFDADGATSTALVV
                     RHLRLLGFEQPDFLVPDRFRFGYGLTPEIVRVAADRRPDLIVTVDNGISSVDGVEEAR
                     RCGIDVLVTDHHLPGRELPRACCIVNPNAPGATFGSKALAGVGVAFYVMAALTHAMHA
                     AGRVPAGVDASPAQWLDLVALGTVADVVPLDLNNRVLVAQGLRRIRRGRCVPGLRALL
                     ELAGRELAGVTGQDLGFQAGPRLNAAGRLDDMSRGIRCLLTDDPVEARGLAAELAQLN
                     TDRRELEARMQFEAVAHVDARVATLEGRVPAGVCVFDEGWHQGVIGLVASRVKERLHR
                     PVIAFAPSEPGWIKGSARSVPGVHVRDVLDAVATGNPGMLDKFGGHAMAAGLALRTEH
                     LPAFESAFAAEVERRVDDDTLAGQLHTDGELLAGEFCVETAAALREGGPWGSGFPEPV
                     FDGRFEVFDARVVADRHLKLRLRCASGEAVEAIAFRHYDDARAPAIRPQDRVELVYRA
                     GVDEYAGQARLQLLVEYVAPLASDRKC"
BASE COUNT          677 a         1623 c         1610 g          764 t
ORIGIN      
        1 tgcccttgcc gcccagctcc ttggccttcc ccgactcggt catgccgtcc cccatctggt
       61 cgtttccgtg acgggtgagg ggtgcccccg ggcgggggtc gccgaaaccg gtgggtcagc
      121 tcctcatcgc cgggaagagc acgacctcgc ggatggtgtg gcggtcggtg aggagcatca
      181 cgaggcggtc gatgccgatg ccgagcccgg ccgtcggcgg caggccgtac tcgagcgcac
      241 gcacgtagtc ggcgtcgtag aacatcgctt cctcgtcgcc gccgctcttg cgcgcggcct
      301 gtgcgcgaaa gcgctcggcc tggtcctcgg cgtcgttcag ctcggagaag ccgttggcga
      361 tctcgcggcc gccgacgaag aactcgaagc ggtcggtcag gaagggatcc gagtcgttgc
      421 gacgcgcgag cggtgacacc tcggccgggt acgcgtacac gaacgtaggc tgcgcgagac
      481 ggtgctccgc ggtcttttcg aagatctcga cctgcagctt gccggcgccg tcgtccggct
      541 tgcagggaat cgccagcttg ccacaccact cgcgcaggta ctgcacgtcg cgcagcagcg
      601 cgcgctcgat gcccggattg aagtgcacga cgaggtcttc gatggtcacg cgtcggaacg
      661 tcgatccgaa atcgaacgtc tcgccctggt attcgacgcg gcgcgtgccg cagatcgtgt
      721 ccgcgacgcc ctggaacatg cgctcgacca ggtccatcag gtcgcggtag tccgcgtagg
      781 cctggtagag ctcgagcatc gtgaactcgg gattgtggcg ggtcgagagc ccctcgttgc
      841 ggaagttgcg gttgatctcg tagacgcgtt cgaagccgcc caccaccagg cgcttcaggt
      901 acagttccgg cgcgatgcgc aggtacatgt ccacgtcgag cgcgttgtgg tgggtcacga
      961 acggcttggc gaccgccccg cccgggatca cctgcaacat cggcgtctcg acctcgagga
     1021 agtcgagcga gtcgaggaag tggcgcagga actgcacgat gcgcgtgcgc acgcggaaga
     1081 cttcgcggct ctcctcgctc gtgatcaggt cgacgtagcg ctgccggtaa cgctgttcga
     1141 cgtcggcgag gccgtgccac ttgtcgggca gcggccgcag cgacttgacc agcagccgca
     1201 ggcgctcgac cttgaccgac agctcgccgg tacgcgtctt gaacagcgtc ccctcggcgc
     1261 cgacgatgtc gccgacgtcc cagcccttga agtcgtcgta aacctcaccg agtgccgact
     1321 gctgcaggaa cagctggatc gagccgctgc ggtccgtgag cctggcgaag ctcgccttgc
     1381 ccatgatgcg cttggccatc atgcggccgg ccaccgcgac gcggatcatg tgggcgtcga
     1441 accactccgc cgcgtgagca ccgtacgccg tctgcaggtc gtccgcggcc gcgttgcgcc
     1501 ggaagtcgtt cgggaacgcg ttgcccgtct cgcgcagccg ctcgagtttc gccttgcgct
     1561 cggcgatgag gtggttctcg gcgtcggccg agacgtcggt gactgccggg gacggttgtt
     1621 cagcgtcgga catcggtgcc tctcgacgcg agcggttagc ggttagcggt tagcggttag
     1681 ttcaagagct cgctcgcgaa cgccgcggcc gctagtgcgc tcctactaac cgctaaccgc
     1741 taaccgctaa ccgctgcgct ccattacaaa ccttgcttca gtgactcttc caggaacggc
     1801 ccgaggtcgc cgtccagcac ggccgtggtg ttgccgatct cgatgccggt gcgcaggtcc
     1861 ttgatgcggg actggtccag cacgtacgag cggatctggt tgccccagct cacgtcggac
     1921 ttcgagtcct cgagcacttt cgcggccgcg ttgcgcttgt tgacctcgag ctcgtagagc
     1981 ttggccttga gcatcttcat cgcggtcgag cggttcttgt gctggctgcg ctcgctctgg
     2041 cacgcgacga cgatgcccga gggcaggtgg cggatgcgca ccgcggactc cgtcttgttg
     2101 acgtgctggc cgccggcgcc actcgatcgg tagacgtccg tctcgaggtc cgcggggttg
     2161 atgtcgatgt cgatgtcgtc gtcgatctcc ggcgacagga acacggacgc gaacgacgtg
     2221 tggcgacgcg cgttcgagtc gaacgggctc ttgcgcacga gccggtgaac gccggtctcg
     2281 gtgcgcagcc agccgtaggc atactcgccg tcgaagcgga tcgtggcgct cttgatgccg
     2341 gcgacctcgc cgtcgttgag gtcgatgact tcggtcttga agccgttctt gtcgccccag
     2401 cgcaggtaca tgcgcatcag catctgcgcc cagtcctgcg cctccgtgcc accggcgccg
     2461 gcctggatgt ccaggaaggc gttgtgcgag tccatctcgc ccgggaacat ccgcttgaac
     2521 tcgagcttct tgacttcgcg gtccacccgc gcgaggtcct gctggacggc cgcggcggtg
     2581 ccttcgtcgg actcggcttc ggccaggtcg agcagctcgc cggcctcggc caggtcatgg
     2641 gtgagcttgt cgagcgtcgc gacgacgccg tcgagtttgg tgcgctcgcg gccgagctcc
     2701 tgggcgcgct cggggttctg ccagatccgc gggtcctcga gctcgcgcag gacctcggtc
     2761 aaccgctcgt gcttgccctc gtagtcaaag gtaccccctg agcgcatcgg tgcgctgcgc
     2821 gaggtcttcg acttggcggc ggatcggatt gatttccatg gacggtggtc ggtgcggaac
     2881 gaactgcgaa gtctagcatt tccggtcact tgcgagaggc gccacgtact cgaccagcag
     2941 ttgcagccgg gcctggcccg cgtactcgtc gacccccgcc cgatacacga gctccacgcg
     3001 atcctgcggc cggatcgccg gcgcgcgcgc gtcgtcgtag tggcggaagg cgatcgcctc
     3061 gaccgcctcg ccggacgcgc agcgcaggcg cagcttgagg tgacggtcgg ccacgactcg
     3121 cgcgtcgaac acctcgaaac ggccgtcgaa caccggctcc ggaaatcctg agccccacgg
     3181 ccctccctcg cgcagggccg ccgccgtctc gacgcaaaac tcgccggcca gcagttcgcc
     3241 gtccgtgtgc agctggccgg cgagcgtgtc gtcgtcgacc cggcgctcga cctcggccgc
     3301 gaacgccgat tcgaatgccg gcaggtgctc cgtgcgcagc gccaggccgg ccgccatcgc
     3361 gtggccgccg aacttgtcca gcatcccggg attccccgtc gccacggcgt ccagcacgtc
     3421 gcgcacgtgc acgccgggga ccgagcgtgc cgagcccttg atccagccgg gttcggacgg
     3481 cgcgaacgcg atgaccggcc gatgcagccg ctccttgacg cgggacgcga cgaggccgat
     3541 cacgccctgg tgccagccct cgtcgaacac gcagacgccc gcgggtacgc gaccctcgag
     3601 cgtggccacg cgcgcgtcga cgtgcgcgac cgcttcgaac tgcatcctgg cttcgagttc
     3661 gcggcggtcg gtgttgagtt gcgcgagctc ggcggcgagc ccgcgcgcct cgaccggatc
     3721 atcggtcagc agacagcgga ttccgcggga catgtcgtcg aggcggcctg ccgcattgag
     3781 acgcggaccg gcctggaaac cgaggtcctg ccccgtcacg cccgccagtt cacggccggc
     3841 gagttcgagc agcgcgcgca gccccggcac gcagcggccg cggcgtatgc gccgcaggcc
     3901 ctgggcgacc agcacgcggt tgttgaggtc gagcggcacg acgtccgcga ccgtgccgag
     3961 cgcgaccagg tcgagccact gggccgggct tgcgtccacg ccggcgggca cccgacccgc
     4021 cgcgtgcatc gcgtgcgtca gcgcggccat cacgtagaac gccacgccga cgccggcgag
     4081 cgccttcgag ccgaacgtcg cgcccggcgc gttcggattg acgatgcaac aggcgcgcgg
     4141 cagttcccgc cccggcaggt ggtgatcggt gaccagcacg tcgatgccgc agcgccgtgc
     4201 ttcctcgacg ccgtcgaccg aggagatccc gttgtccacc gtgacgatca ggtccggtcg
     4261 gcggtcggcc gcgacgcgca cgatctcggg cgtcagcccg tagccgaacc ggaaccggtc
     4321 gggcaccagg aagtcgggct gctcgaagcc cagcaggcgc aggtgccgca cgaccagcgc
     4381 ggtgctcgtc gcgccgtccg cgtcgaaatc gccgatcacc agtacgcgcg cccgctgccg
     4441 atgcgcggcg accagccgct cggcggcctg ctcgaccccc tcgagcgacc gaaccggcaa
     4501 cagctggtcg aggcgcagcg cgaggtcgtc ggcgtgcgtc acgccgcgcg cgccgtacac
     4561 gcgccgcagc acgggatgca gcgacgcggg caacgccgcc tgcagctccg cacgcgggtc
     4621 gcggcggacg atgcgacgcg ccggtgtcga catcaaaggt ccgccggccg ggcg
//
LOCUS       DASPFR010000183         5571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_174806, whole genome shotgun sequence.
ACCESSION   DASPFR010000183 DASPFR010000000
VERSION     DASPFR010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174806"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2261
                     /locus_tag="VD737_04650"
     CDS             <1..2261
                     /locus_tag="VD737_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXW09881.1"
                     /translation="DAPEPASAPSPDLFTAINMTGASIAQNELGLTGEGVKVAVMDTG
                     IDIEHPAFGGSGVPLTGTGTFPTARVVEGWDFVGDAFNADPTSLAYNPTAAPDPNPDD
                     CNGHGSHVAGIVGANDATNGMKGVAPGVTFGAYRVFGCDGSTTSDIMVAAMERALADG
                     MDVLNMSIGSSYQWPQYPTAAAADRLVNKGMVVVTSIGNAGANGLYSASAPGVGAKVI
                     GTASFDNTNQMLPTFTISPDDTSIGYTNATAAPPAPTSGSLPMARTGTSTTTNDGCAP
                     HAAGAYTGQAVLIRRGTCGFYDKALNAQNAGAAAVVLYNNVAGRINPTVAGAMPITIP
                     VVAISDTEGVLINNRLAAGPVTLTWTDDLGSFLSATGGLISSFSSYGLSPDLTVKPDI
                     GAPGGNIYSAYPLEKGGYANISGTSMASPHVAGGVALLVQANPKTPSQVVRTILQNSA
                     DPKNWWGNPALGFLDNVHRQGAGMVDIDDAILATTRIEPAKLALGESQAGPATRTLTI
                     RNDGDAAVTYDLSHVPALSTGPNTFTPAFVTGFATVAFSAPSVTVPAKGSASVDVTIT
                     ANAGLADLSQYGGYLVLTPQGGGQVYRVPYAGLKGDYQAKPVFTGGFPFLGRLTACSL
                     VRDLDCYVGGSYTVPAAGTVYSMTNAFNVPQLLVHLDHQVRLFRVEIFDSNGKAWHRE
                     YNEDYVGRNSTATGFFTFALDGVTTAGNKSYTLPEGTYTAKVSVLKALGDAANPAHWE
                     TWTSPAFVIDRP"
     gene            complement(2435..4570)
                     /locus_tag="VD737_04655"
     CDS             complement(2435..4570)
                     /locus_tag="VD737_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXW09882.1"
                     /translation="MTTPIRVPATIAALVAIATLAPAQAATAPKPDDPTSFLAAIKQA
                     PDAKLGPWLANLAAEYRAARDKGVTDTRFRTKNRALRTSRGTVAIDAVANNPAALVRS
                     LKAMGAVSVTAKGPLVAARVPISALGRLAAEPSLRYARPVMATKDALPANAVSQGVVS
                     LRADVGREQAGVDGSGIKVGVLSDSIGCNPPPFLPGTRTTTLQEDINTGELPTEVQIL
                     KDAVCPATDEGRGMAQLIHDTAPGAAIAFHTAFESELDFAEGIIRLQENAGSDVIVDD
                     VRYFAEPFFMDGMIAQAVDIVARRGVPYFSSAGNQARNSYESAYRPVNQAINASGNVT
                     GAPNRVSRMHDFDPGPGVQVLQPVAVVPDADAGFIIFSFQWDQPHRTATTYAWIKEGK
                     SAAEASQLAKGAASDLDLVIFDHKGHLLRRCPPGVSTGITCQITGDSNVGGDAVDLAA
                     IFYSGPPKAPQLFYIAFVHSAGPDPGVVKYSWFDSQGAMVPLDFHTASGTSFGHSNAA
                     GNIAVGAAAWYATVPFSTSGNYPPNDIYFTPKIAPGLAVCAPACLNDFSSAGGIPIYF
                     DRFGNRLAAPEVREVPAVTGPDGGNTSFFTTDSTYDDDDGDGLNSPFSTFVTPQLDSG
                     LDEFPNFFGTSASAPHVAAVAALLLDKTASLTPEQVREVLQKTARGPIDKRFTSNRPI
                     IITPIEVGGYNFDAGTGLVDAAAALDAAD"
     regulatory      4897..4980
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            5108..>5571
                     /locus_tag="VD737_04660"
     CDS             5108..>5571
                     /locus_tag="VD737_04660"
                     /inference="COORDINATES: protein motif:HMM:NF017709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I9 family protein"
                     /protein_id="HXW09883.1"
                     /translation="MSKKPLLTLLSSAALSAVAALSVPISAGVAAPAAASENARAIKG
                     SYIVQLSESPVVAYTGDIKGYAATKPRAGQKIDPFSSKVVNYKSYLESRHDAVLASVG
                     GKKFYSFGYSFNGFAARMTEAQANALASTKGVVKVTQDAIHLVDTSSTPDFL"
BASE COUNT          934 a         1835 c         1893 g          909 t
ORIGIN      
        1 tcgacgctcc ggaaccggcc tcggcaccgt cgccggacct gttcaccgcg atcaacatga
       61 ccggcgccag catcgcgcag aacgagctcg ggctcacggg cgagggcgtc aaggtggccg
      121 tcatggacac cggcatcgac atcgagcacc cggcgttcgg gggttcaggg gtgccgctca
      181 ccggcacggg aaccttcccg accgcgcgcg tcgtcgaagg atgggatttc gtcggcgacg
      241 ccttcaacgc ggacccgacg tcgctggcct acaaccccac tgccgcgcct gacccgaacc
      301 cggacgactg caatggccac ggttcgcacg tggcgggcat cgtcggcgcc aacgacgcga
      361 ccaacggcat gaagggcgtg gcgcccggcg tcacgttcgg ggcgtaccgc gtattcggct
      421 gcgacggctc gacgacgtcg gacatcatgg tcgccgcgat ggagcgcgcg ctggccgacg
      481 gcatggacgt gctcaacatg agcatcggtt cgtcgtacca gtggccgcag tatccgacgg
      541 cggcggcggc ggaccggctg gtgaacaagg gcatggtcgt cgtcacctcg atcggcaacg
      601 cgggtgccaa cggcctgtac tcggcgagcg cccccggcgt cggcgccaag gtgatcggca
      661 cggcgtcctt cgacaacacc aaccagatgc tgccgacctt cacgatctcg ccggacgaca
      721 cgtcgatcgg ctacaccaat gcgacggctg cgcccccggc gccgacgtcg ggcagcctgc
      781 ccatggcccg gacgggcact tcgacgacca ccaacgacgg gtgcgctccc cacgcggccg
      841 gcgcctacac cggccaggcc gtgctgatcc ggcgcggcac ctgcggcttc tacgacaagg
      901 cactcaacgc acagaacgcc ggtgccgcgg ccgtcgtgct gtacaacaac gtggcgggcc
      961 gcatcaatcc gacggtcgcg ggcgcgatgc cgatcacgat tccagtcgtg gcgatatccg
     1021 acaccgaggg cgtgctgatc aacaatcggc tcgccgcggg cccggtcacc ctgacctgga
     1081 ccgacgatct gggttcgttc ctgagcgcga cgggcggact gatctcgtcg ttcagctcgt
     1141 acggcctgtc gcctgacctc accgtgaagc ccgacatcgg cgccccgggc ggcaacatct
     1201 actcggccta tccgctcgag aaaggcggct acgccaacat cagcgggacc tcgatggcct
     1261 cgccgcacgt ggcgggcggc gtcgcgctgc tggtgcaggc caatccgaag acgccgtcgc
     1321 aggtcgtccg caccatcctg cagaacagcg ccgacccgaa gaactggtgg ggcaacccgg
     1381 ccctcgggtt cctcgacaac gtgcatcgcc agggtgccgg tatggtcgac atcgacgacg
     1441 cgattctcgc gacgacgcgg atcgagccgg cgaagctcgc gctgggtgag agccaggcgg
     1501 gaccggcgac ccggacgctg acgatcagga acgacggcga tgcggccgtc acctacgacc
     1561 tgagccacgt gccggcgttg tccaccggac ccaacacgtt cacgccggca ttcgtcacgg
     1621 gcttcgccac ggtagccttc agcgcacctt ccgtgacggt cccggccaag ggctcggcct
     1681 cggtcgacgt gacgatcacg gccaatgcgg gcctggcgga cctgagccag tacggcggct
     1741 acctggtctt gaccccgcag ggcggtgggc aggtatatcg agtgccttac gccggcctga
     1801 agggggacta ccaggccaag ccggtcttca ccggtggctt cccgttcctg ggcaggctca
     1861 cggcgtgctc cctcgtgcga gacctcgact gctacgtggg cggctcgtac accgtccctg
     1921 cagccggaac ggtctacagc atgacgaacg cgttcaacgt gccgcagctg ctcgtgcacc
     1981 tcgatcacca ggtccgcctg ttccgggtcg agatcttcga ctcgaacggc aaggcgtggc
     2041 atcgcgagta caacgaggac tacgtcggtc gcaacagcac cgcgaccggg ttcttcacgt
     2101 ttgcgctcga tggggtcacg accgcgggca acaagagcta cacgctgcct gaaggcactt
     2161 acaccgcgaa ggtgtcggtg ctcaaggccc tgggcgacgc cgcgaatccc gcgcactggg
     2221 agacgtggac ctcgccggcg ttcgtgatcg accggccgta acggcagcaa gaccggagcg
     2281 agggcccctc ggaaacgagg ggcccttttt ttcgtagctc gatcacgagg gcgacgtcac
     2341 ctgtgttcgc gcggaacacg tgcattcacc gcgaaaaaaa agagcccggt cacgggaccg
     2401 ggctcgaggc tcaagtcgta atgggaatgc gacgtcagtc tgcggcatcc aaggcggccg
     2461 ccgcgtcgac gaggcccgtg cccgcgtcga agttgtagcc gcccacctcg atgggcgtga
     2521 ttatgatcgg ccggttcgac gtgaagcgct tgtcgatcgg cccgcgcgca gtcttctgca
     2581 gcacctcgcg cacctgttcc ggtgtgagcg acgcggtctt gtcgagcagc agcgccgcca
     2641 ccgccgcgac gtgcggggcc gaggccgagg tgccgaagaa gttcgggaac tcgtcgagcc
     2701 cactgtcgag ctgcggcgtc acgaacgtgc tgaacgggct gttgaggccg tcaccgtcgt
     2761 cgtcgtcgta ggtcgaatcg gtggtgaaga agctcgtgtt gcccccgtcg gggccggtca
     2821 cggccggcac ttcacgcacc tccggcgccg cgagccggtt gccgaaccgg tcgaaataga
     2881 tcggaatgcc accggccgac gagaagtcat tgaggcaggc cggcgcgcag acggcgagtc
     2941 caggcgcgat cttcggcgtg aagtagatgt cgttcggcgg gtagttgccg ctggtgctga
     3001 acggcaccgt cgcgtaccag gccgccgcgc cgacggcgat gttgccagcg gcgttcgagt
     3061 gcccgaacga ggtgccgctc gcggtgtgga agtcgagcgg caccatcgcg ccctggctgt
     3121 cgaaccagct gtacttgacg acgcccgggt ccgggccggc cgagtgaacg aacgcgatgt
     3181 agaacagctg cggcgccttg ggcgggccgg agtagaagat cgccgcgaga tccaccgcgt
     3241 cgccgcccac gttgctgtct ccggtgatct ggcaggtgat gcccgtggat acgccgggcg
     3301 ggcagcgtcg cagcaggtgg cccttgtgat cgaagatcac gaggtcgagg tcgctggccg
     3361 cgcccttggc gagctgcgac gcctcggccg cgctcttgcc ttccttgatc cacgcgtacg
     3421 tggtcgcggt gcggtgcggc tggtcccact ggaagctgaa gatgatgaac ccggcgtcgg
     3481 cgtccggcac gacggcgacg ggctgcagca cctgcacgcc ggggcctgga tcgaagtcgt
     3541 gcatgcgact caccctgttg ggggcgccgg tgacgttgcc gctcgcgttg atcgcttggt
     3601 tcacgggccg atacgcgctc tcgtaggagt tgcgcgcctg gttgcccgcc gacgaaaagt
     3661 acggcacgcc gcgtcgcgcg acgatgtcga ccgcctgcgc gatcatgccg tccatgaaga
     3721 acggttcggc gaagtaccgc acgtcgtcga cgatgacgtc ggaacccgcg ttttcctgca
     3781 ggcggatgat gccttcggcg aagtcgagct cgctctcgaa cgcggtgtgg aacgcgatcg
     3841 ccgcgccggg tgccgtgtcg tggatcagct gcgccatgcc gcggccttcg tcggtggccg
     3901 ggcagaccgc gtccttgagg atctgcacct ctgtcggaag ctccccggtg ttgatgtctt
     3961 cctgcagcgt ggtcgtgcgc gtgcccggca ggaacggcgg cgggttgcag ccgatgctgt
     4021 ccgagagcac gccgaccttg atgccgctgc cgtcgacgcc ggcctgctcg cggcccacgt
     4081 cggcccgcag gctgacgacg ccctgcgaga cggcattggc cggcagcgca tccttcgtcg
     4141 ccatgaccgg ccgtgcatag cgcaacgacg gttcggcggc gagccggccg agtgcgctga
     4201 tcggtacgcg cgccgccacg agcgggccct tcgcggtcac ggagaccgcg cccattgcct
     4261 tgagcgagcg caccagggcg gcaggattgt tggcgacggc gtcgatcgcg accgtgccgc
     4321 ggctcgtgcg cagggcgcgg ttcttcgtgc ggaacctggt atcggtcacg cccttgtcgc
     4381 gtgccgcacg gtattcggcc gcgaggttcg cgagccaggg acccagcttt gcgtcgggcg
     4441 cctgcttgat ggctgcgagg aacgacgtgg gatcgtcggg cttcggtgcg gtggccgcct
     4501 gggccggggc gagggtcgcg atcgcgacca gcgctgcgat cgtggcgggc actcgtatcg
     4561 gtgtcgtcat tcaccaactc cttgtgaaaa acgtcagcgg tgaaccgttc attccgctga
     4621 ccggggcggg cgagcgtact ccgcggttga catgatcgcg aggggtcaag cgatgtaatt
     4681 tcgcaacaaa cgccgggaaa aacagtgtcg aagtcatttc ggcaagctcg cctcgttatg
     4741 ctgcggagca gcgaagggat aggctcggcc ggtgccgaat tggtaccggc aacccgcgcc
     4801 actgccggtg actaccgcgt tatgtcatct cgtccggaat aaacatatcg atgtcgttgc
     4861 tttcgcttag cgacgaggtt agtttcgccc aacggcaagc gtgaagggca cacccgccgc
     4921 gagacggggc cgcaaagcca cagctcttga tcaatagaac aagagggctg ggctgccgga
     4981 agggaggcac cgcagacgtc gcaatgcccg cgaggtccgg tggttttttt tcctgtcgag
     5041 gacgtccgtc ctcgcgtctg cccgacgttc aaacacggtg aaatcctcgt cgggagggga
     5101 acattccatg tcgaagaaac cattgctgac gctgctgtcg tcggcggcac tgagcgccgt
     5161 cgcagcactg tcggtgccga tcagtgccgg agtcgccgct ccagccgcgg catccgaaaa
     5221 tgcgcgggcc atcaaaggct cgtacatcgt tcagttaagc gagagccccg tcgtcgccta
     5281 caccggcgac atcaagggtt atgcggcgac caagccgcga gcgggccaga agatcgatcc
     5341 gttcagctcg aaagtcgtca attacaagag ctacctcgag tcgcggcacg acgcggtgct
     5401 cgcctcggtc ggtggcaaga agttctacag cttcggttac tcgttcaacg gcttcgccgc
     5461 caggatgacc gaggcccagg cgaacgcact ggcttcgacc aaaggcgtgg tcaaggtcac
     5521 gcaggacgcg atccatctcg ttgacacgtc gtcgactccc gacttcctcg a
//
LOCUS       DASPFR010000184        10677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_786079, whole genome shotgun sequence.
ACCESSION   DASPFR010000184 DASPFR010000000
VERSION     DASPFR010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10677
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786079"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1893
                     /locus_tag="VD737_04665"
     CDS             <1..1893
                     /locus_tag="VD737_04665"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXW09884.1"
                     /translation="DGDSLIRPADNPSEARGVLSTSQPTVVLLDARGLEDLAAVVGDL
                     QSPDGARIVVIFATAEQSDDVARSVRGSAAFAVLAIPVVPPQAAAVLEGACEEARARH
                     ALLQGPTSTPPPPVVDARPETSPVELAPETCAPSPSSRLQVQASSSAMTRKSTALIGV
                     GVVGLLVIAVSVAWWLQEPVPATGQAAPSRAVSVAPAALVEPELEPQAVQAGSIDELL
                     DSARTAMRERRYTDPENRSAYSYYRSVLAQDPGNGEAREGLERIATVLQERVQASIEE
                     QRWEEAGRTLAQLRAIRPAAPEVAGFDATLREAHARSQAEIERRQVGERADQLADLVS
                     LRIRQGKLLDPANDSARHYLAQLRRLPGDVPRAGTATTELQQAYLGRLRDALAKSQRT
                     EAERWKAAARGLGVSATELSAVQRDVAARAALSESKQEGARIAQLVQQRIADGRLLEP
                     ASDSAVFHLNALRALDPAAAIVASNERALSGRLVEHARFALAEQRLDDARTHAAAARQ
                     LGVDSDVVAALERDIANAGATPGPAGARPAPRLVRTRYVAPQYPAAALKQGLTGSVRL
                     RLAVNAEGHVSEAMVVQASPPGIFDAAAVNAARKWRFKAIGPKGSEVHATATVDMLFK
                     PEDAQQ"
     gene            1890..3980
                     /locus_tag="VD737_04670"
     CDS             1890..3980
                     /locus_tag="VD737_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXW09885.1"
                     /translation="MKPVARFREGAVTIGFLALLVAFIGAVLWPGYRLADRLAASNAA
                     LRLASEQQGRPEAIASALESVRDRLGAGAYVGESIADLHRTMTSFDSALTELRGSAAG
                     ASAELRSAEALWARYQAVLGPVATFEGLPYRDTDETGTAMTAAGRELGADTRGALAMA
                     RIATPKLTEALTSIGARLQRDAAADSARLRLLMIVGVAFACAFGASFAYVQWLKRRHE
                     RAALEARHQTRDILATVKDGLFLIDAEFRVGKAHSAALGTLLGRETFDGLTFEDLLRP
                     LVPDKTLATASKYVKLLWGERANENLIRSINPLSEVEVQIDRGEGRRDVRYLDFEFHR
                     VRGDEGTRQVLVTVDDVTSRVLLARELKESQANAQSQLDMLLGILQADPAQALGFLDD
                     AGAALGHVNSVLKVPARNDREFRDKIDQLFREIHRIKGEAATLGLVALENRAHDFEDA
                     LNELRQRTNLSGDDFLPMVVRLDELFSHLKSVRELVVRLDGLRVNAVLEAPATPIPSL
                     SEAAFDRLAQRLAADHGKDVRVTCMGLEHVPADYARPLRDIAIQLVRNAVVHGIEAPA
                     ARRTAGKAATGALRLEFHAAPGGYELLFEDDGAGVDERRVRAAAVARGLVAAEQGEAL
                     DSRAVLALLFKAGFSTRDRQDRDAGRGVGLDLVRRTVQSLNGKLGVTSVPGRFTRFRL
                     ALPSLEADAQQAAG"
     gene            4003..4398
                     /locus_tag="VD737_04675"
     CDS             4003..4398
                     /locus_tag="VD737_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXW09886.1"
                     /translation="MPAAAPRQDRPLRLLIVDDSNVIRRRIERSQQIARLEVVGTAND
                     GVTAIEEFRRTDPDVVTMDITMPQMDGIECVERLVSMKPHVLILVVSALADKATAVEA
                     MEKGANGFLNKPFTDRQLNDALLELIAGA"
     gene            4406..4855
                     /locus_tag="VD737_04680"
     CDS             4406..4855
                     /locus_tag="VD737_04680"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HXW09887.1"
                     /translation="MMEERDIRAFVFGTTRYFEVAAQQAATVGSPFLVTQGAPEVHDY
                     TGVIEVSGRRRGVVYFSAPRGMLTVLLMKMNESDVSHENLCDLVGEVANTISGNVRRD
                     FGKDFTISPPSVVTGHAAPITLPSGCRPVVIPIHWRSHAARLVVCLQ"
     gene            complement(4873..6684)
                     /locus_tag="VD737_04685"
     CDS             complement(4873..6684)
                     /locus_tag="VD737_04685"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HXW09888.1"
                     /translation="MPSPHAVAVLIVTIAVFYLYTRRWIRMELVSLLLLALLLSIFYL
                     FPYVSPDHRITEAEVLQAFGHPALVAICSLMVLGRGLTMTGALEPIVRLIARVWQFNT
                     AIGLLLTLTLAAFVSGFINDTPVLVMMLPMLLSLADRTGYPSSRTLMPVNFAILIGGM
                     LTSIGTSTNLLVLSIAADFGMRPMGVFDFTATTSIALLVALPYLWLVAPRLLSTKQGE
                     PQEHAREFDARVVAEGRNGKLIGRDRSGLRRTLGREMPLVELIRGGERLSADAFGTVV
                     AGDVLVLRDTPAGLRDVAATFGAELFDRTRREPFIAADRDDDVSLGEVVIGTACSLVG
                     STLSQARFAERFHVVVVGLKRGTEQILRDGLPIQDVPLTAGDVLLVQGPDRRLRALKS
                     EPHLMVLDSTLQLPRSPLAWRALLIMFLVVLAAATRVMPIHVAAFLGVIAMLLAGCVK
                     FEGLGRALSPAVVLMVASSVALGHSIVTTGAADWIGAGISTLVESASPAVQIAVFMGF
                     SALLTNFVSNSAAAAVGTPIAVATAIQLGAPLEPFVLAIMFGANLSFATPMAYQTNLL
                     VMNAAGYTFRDFIRVGLPLVVIMLVTLSVLLARRYGF"
     gene            6888..7460
                     /locus_tag="VD737_04690"
     CDS             6888..7460
                     /locus_tag="VD737_04690"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HXW09889.1"
                     /translation="MQATQKHAGKAGMGWGRFAAMIATSTVIMFPLMYQLVYRADHLT
                     FSVTRLLSALIMGAVMTIVMLGFMWSMYEGTRTKIAVMAGAALLGCVLFLLNRSQALI
                     DDTAFMQAMIPHHSIAINNARKARIHDPRVRELADQIISSQVREIREMELLVEDIERD
                     GRLGERVLPARAAVITPEMEREVQAAVRRD"
     gene            7600..9351
                     /locus_tag="VD737_04695"
     CDS             7600..9351
                     /locus_tag="VD737_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HXW09890.1"
                     /translation="MHDTPRRWVWAGLASAAFVPGAHAGIEVEIDGLDDDEIRGAVRA
                     ALTLTQYKRRDVTLARARYLYGQAEEEIKRALEPFGYYDPGVEGRFETIEAGEYRVTF
                     SVRPGPAVQVTRRSLELPEDVRKLEPVEAALIEFEPERGERLDHQLYERSKQRVTTAL
                     RAVGYLDAETSVHQVAVNTAQRTAEIDLAWELGPRYRLGTVQFSDSQFDDRFLSGYVP
                     WQPQDYYSTEALLTLQQRLSQTGYFQTVAVEPVLDERGDGVVPVQVVLTENERNLYRA
                     GVYYSTDYGVGGRLGYERRWLNRRGHTLDTNAEYSQRLQDYSVTYEIPRPSPEDKRYT
                     FGAAYRDETSDTAQSRTLRLAAADSRKEWKGFARTLGLHYLDGDFEVGGERGRSALLF
                     FEGTLGRKHYDDLAFPRDGYSFDIGLRLAPGSGLSDTSLAQAWVQHKRLLRQGSDHSR
                     IILRARLGAMAVGDFDELPPDLRFFAGGDRSIRGFDYQAIGELNDQGKVVGGQYLAVA
                     SAEYEYYLPQRENWGAAVFTDIGDAFTHDFRENVSVGVGLRWRSPIGMVRVDVATPVE
                     SRFDDGVRLHITIGPDL"
     gene            9348..>10677
                     /locus_tag="VD737_04700"
     CDS             9348..>10677
                     /locus_tag="VD737_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09891.1"
                     /translation="MTDTRGPPSDTPGQDGERASRATHRGRRIAGYVLLAVVALLVLI
                     AAVVAWIVLTPGGTRWAVGQAESRLPALRIEHVEGTAGHGLTLRGLRYEPEQGTRVRA
                     ALARIQLDLGALWKRSVVIEDLFLQGARVQIAESPDQEPQEPFSLEPPVDVIVARGRA
                     EDVIVADETGAIATIRHAELRGGWTRANGIEVEQLRVAAREGDVRLQGHLDGGPGQYR
                     ADGDASVRWQVGARTYAGTAHVSTDDARIRVETRLTSPLAARATGSFDQQAGQWAAGL
                     RVPAVDLRAEMAPDAPVGVVSAELHANGTERTAAVQGELVVDGETVHVVDGRLVLRPE
                     PRTLHLERLVLRLNEDPGQFVASGTFALDADRVTADLKTHWRELTIPEAWAGQQLHTN
                     GQLAVRGSAERYALSGEIEVGPPGRLADVSVVAQGTPRRLALEKFVVEQEA"
BASE COUNT         1726 a         3655 c         3770 g         1526 t
ORIGIN      
        1 gacggtgact cgctgatccg ccccgcggac aatccttccg aagcccgtgg cgtgctctcg
       61 acgtcccagc cgaccgtcgt gctgctggac gcccgcggcc tcgaggatct cgcggcggtc
      121 gtcggcgatc tgcagtcgcc agacggcgcg cggatcgtgg tgatcttcgc gacggcggag
      181 cagagcgacg acgtcgcccg cagcgtgcgt ggctcggctg cgttcgccgt gctcgcgatc
      241 ccggtcgtgc cgccgcaggc ggccgccgtg ctcgaaggcg cgtgcgagga ggcgcgcgct
      301 cgtcacgcgt tgctgcaggg cccgacgtcg acgcctccgc cgccggtcgt cgacgcgagg
      361 ccggaaacct ccccggtcga gctggcaccg gagacctgcg cgccgtctcc gtcgtcgcgc
      421 ctgcaggttc aggcatcgag cagcgcaatg acgcgcaagt cgaccgccct gatcggggtc
      481 ggcgtcgtgg ggctgttggt catcgccgtc agcgtggcct ggtggctcca ggagccggtg
      541 cctgcgaccg ggcaagccgc cccgtcacga gccgtcagcg tcgcgcctgc ggccctcgtc
      601 gaaccggagc tggaaccgca ggccgtgcag gccggcagca tcgacgaact gctcgactcg
      661 gcccgtaccg cgatgcgcga gcgacgctac accgacccgg agaatcgcag cgcgtacagc
      721 tactaccgtt cggtgctcgc ccaggatccc ggcaacggcg aggcacgcga aggcctcgag
      781 cgcattgcca ccgtgctgca ggagcgcgtg caggcgtcga tcgaagagca gcgctgggag
      841 gaggcgggcc gcacgctcgc gcagctgcgc gcgatccgtc ccgcagcccc cgaggtcgcc
      901 ggtttcgacg ccacgttgcg cgaggcgcat gcccgctcgc aggccgagat cgagcgccgc
      961 caggtcggcg aacgcgccga ccagttggcg gacctcgttt cgctgcggat ccgccagggc
     1021 aaattgctgg atccggcgaa cgacagcgcc cggcactacc tggcgcagct gcgtcgcctg
     1081 ccgggcgacg ttccgcgcgc cggcacggcg accaccgagc tgcaacaggc ctacctcggc
     1141 aggttgcgag acgcgctcgc caaatcgcag cgcacggagg ccgagcgctg gaaagcggcg
     1201 gctcgcggcc tgggcgtgag cgcgacggaa ctctcggcgg tccagcgtga cgtcgcggca
     1261 cgggccgcgc tgtccgaatc gaagcaggag ggcgcgcgca tcgcacagct cgtgcagcag
     1321 cgcatcgccg acggtcggtt gctcgagccg gccagtgaca gcgcggtctt ccacctgaac
     1381 gccctgcgcg cgctcgatcc cgccgcggcg atcgtcgcca gcaacgagcg ggcattgtca
     1441 ggccggctgg tggaacacgc gcggttcgca ctggccgagc agcggctcga cgacgcgcgt
     1501 acgcacgcgg ccgcggcgcg ccagctgggc gtcgacagcg acgtcgtggc cgcgctagag
     1561 cgcgacatcg cgaacgccgg cgccacccct gggccggccg gggcgcgtcc ggcaccgcgg
     1621 ctggttcgaa cccgctacgt cgcgccgcaa tatcccgcgg ccgcgctcaa gcagggtctc
     1681 acgggcagcg tgcggctgcg tctcgcggtc aacgccgagg gacacgtcag cgaggcgatg
     1741 gtcgtgcagg cctcgccccc gggaatcttc gacgccgccg ccgtcaacgc cgcgcgcaag
     1801 tggcgcttca aggcgatcgg ccccaagggg tcggaggtgc acgccaccgc gacggtggac
     1861 atgctcttca agccggagga cgcccagcag tgaagcccgt ggcacgtttc cgcgaaggcg
     1921 ccgtcaccat cggcttcctc gcgctgctcg tcgcattcat cggcgcggtc ctgtggcccg
     1981 gttatcggct cgccgatcga ctggccgcca gcaacgccgc gctgcggctc gcgagcgaac
     2041 agcaggggcg acccgaagcg atcgccagtg ccctcgaatc cgtccgtgac cggctgggcg
     2101 cgggcgccta cgtcggcgaa tcgatcgccg acctgcatcg aacgatgacg agcttcgact
     2161 cggcgctgac cgagcttcgc ggctcggccg ccggcgcgag cgcggaactg cgttcggcgg
     2221 aggcgctctg ggcccgctac caggcagtcc tgggacccgt ggccacgttc gagggcctgc
     2281 cttaccgcga caccgacgag accggcacgg cgatgacggc cgcggggcgc gaactgggcg
     2341 ccgacacgcg cggcgcgctc gcgatggccc gcatcgccac gccgaaactg accgaggcac
     2401 tgacgtcgat cggtgcgcga ctgcagcgtg acgcggccgc cgattctgcg cgactgcgcc
     2461 tgctgatgat cgtcggcgtg gccttcgcct gtgcgttcgg tgcgtcgttc gcgtacgtgc
     2521 agtggctgaa acgtcgacac gagcgtgcgg ccctggaagc ccggcaccag acccgggaca
     2581 tcctcgcgac cgtcaaggac gggctgttcc tgatcgacgc cgagttccgc gtcggcaagg
     2641 cacactcggc cgcgctcggc acactgctcg gtcgcgagac cttcgacggc ctgacgttcg
     2701 aggacctgct gcggccgctg gtgcccgaca agacgcttgc gacggccagc aagtacgtca
     2761 agctgctgtg gggggagcgc gccaacgaga acctgatccg gagcatcaac ccgctgtccg
     2821 aggtcgaggt gcagatcgac cgcggcgaag ggcgacgcga cgtccgttac ctcgacttcg
     2881 agttccaccg cgtgcgcgga gacgagggca cgcgccaggt cctcgtgacg gtcgacgacg
     2941 tgacttcacg cgtgctgctc gcgcgcgaac tgaaggagtc gcaggcaaat gcacagtcgc
     3001 agctcgacat gctgctcggc atcctgcagg cggacccggc ccaggcgctc ggcttcctgg
     3061 acgatgccgg cgcggccctg ggccacgtca attcggtgct gaaggtgccg gcccgcaacg
     3121 accgtgagtt ccgcgacaag atcgaccagc tgttccgcga gatccatcgc atcaagggcg
     3181 aggccgccac cctcggcctg gttgcgctcg agaaccgcgc gcacgatttc gaggacgcgc
     3241 tcaacgagtt gcgccagcgc acgaatctgt cgggcgacga tttcctgccg atggtcgtgc
     3301 gtctcgacga gctgttcagc cacctgaaat ccgtgcgcga actcgtcgtg cggctcgacg
     3361 gcctgcgcgt caacgccgtg ctcgaggccc ctgcgacgcc gatcccctcg ctgtccgagg
     3421 cggccttcga cagactcgca cagcgcctgg ccgccgacca cggcaaggac gtgcgcgtga
     3481 cctgcatggg gctcgagcac gtgccggcgg attacgcccg acccctgcgg gacatcgcca
     3541 tccagctggt ccgcaacgcc gtcgtgcacg gcatcgaggc gcccgccgcg cggcggacgg
     3601 cgggcaaggc cgccacgggt gcactgcgcc tcgagttcca tgcagcgccc gggggctacg
     3661 aactgctgtt cgaggacgac ggtgcgggcg tcgacgaacg gcgcgtgcgg gccgctgcgg
     3721 tggcccgcgg cctcgtcgcg gcggagcagg gcgaggcgct cgactcccgg gcggtgctgg
     3781 ccctgctgtt caaggccggc ttctcgacgc gcgatcggca ggaccgtgac gccggccgcg
     3841 gcgtcgggct cgacctggtc cgacgcacgg tccagtcgtt gaacggcaag ctcggggtga
     3901 cgagcgtgcc tggcaggttc acccgattcc ggctcgcgct gccgtccctg gaagcggacg
     3961 cccagcaggc ggccggctga cctgcggcgc ggagtacgcc caatgcccgc tgccgcgccg
     4021 cgtcaggacc gcccgctcag gctgctgatc gtcgacgact ccaacgtgat ccggcgtcgg
     4081 atcgagcgct cgcagcagat cgcgcggctc gaggtcgtcg gcacggcgaa cgacggggtc
     4141 acggcgatcg aggagttccg gcgcaccgac ccggacgtcg tcacgatgga catcacgatg
     4201 ccgcagatgg acggcatcga gtgcgtcgag cggctggtct cgatgaagcc gcacgtgctg
     4261 atcctcgtgg tgtccgcgct cgcggacaag gccaccgcgg tcgaggcgat ggagaagggc
     4321 gccaacggtt tcctgaacaa gccgttcacg gaccgccagc tcaacgacgc gctgctcgaa
     4381 ctgatcgccg gagcctgatc gccccatgat ggaagagagg gacatccgcg cgttcgtgtt
     4441 cgggaccacc cggtacttcg aggtggccgc gcagcaggcc gcgacggtgg gctcgccatt
     4501 cctggtcacg cagggcgccc ccgaagtcca cgactacacc ggcgtgatcg aggtgtccgg
     4561 ccggcgccgc ggcgtcgtgt acttctccgc gccgcgcggc atgctgaccg tgctgctgat
     4621 gaagatgaac gagagcgacg tctcgcacga gaacctgtgc gacctcgtcg gcgaggtcgc
     4681 caacacgatc tcgggcaacg tgcgacggga tttcggcaag gacttcacga tctcgcctcc
     4741 ttccgtcgtc accggccacg cggcgccgat cacgctgccg tccgggtgtc gtcccgtcgt
     4801 gatcccgatc cactggcgca gccacgcggc ccgactggtg gtgtgcctgc agtaggacga
     4861 ggtgcggccg gttcagaacc cgtaacgacg cgcgagcagg accgacagcg tgaccagcat
     4921 gatcacgacc agcggcaggc cgacgcggat gaagtcgcgg aacgtgtagc cggcggcatt
     4981 catcaccagc aggttcgtct ggtaagccat cggggtcgcg aagctcaggt tcgcgccgaa
     5041 catgatcgcg agcacgaacg gctcgagcgg cgcccccagc tggatcgcgg tggccacggc
     5101 gatcggcgtg ccgacggccg ccgccgcact gttcgacacg aagttggtga gcagcgccga
     5161 gaaccccatg aacacggcga tctgcacggc cggggaggcg gactccacca gcgtagagat
     5221 gcccgcgccg atccagtcgg cggcgcccgt cgtcacgatg ctgtgcccga gcgccacgct
     5281 cgaggccacc atcaggacga cggccgggct caatgcgcga ccgaggccct cgaatttcac
     5341 gcagccggcc agcagcatcg cgatgacgcc caggaaggcg gcgacgtgga tcggcatgac
     5401 gcgcgtcgcc gccgcgagga cgaccaggaa catgatcagc agggcccgcc aggccagcgg
     5461 cgagcgcggc aactgcagcg tgctgtcgag caccatcagg tgcggctcgg acttcagggc
     5521 ccgcaggcgc ctgtccggtc cctggacgag cagcacgtcg ccggcagtca gcgggacgtc
     5581 ctggatcggc aagccgtcac gcaggatctg ctcggtgccg cgtttgagcc cgacgaccac
     5641 gacgtggaag cgctccgcga atcgcgcctg cgacagcgtg ctgccgacca gcgagcaggc
     5701 cgtgccgatg acgacctcgc cgagactgac gtcgtcgtcg cggtcggcgg cgatgaacgg
     5761 ctcgcggcgg gtccggtcga acagctcggc cccgaacgtg gccgcaacgt cacgcaagcc
     5821 ggccggcgtg tcgcgcagca ccaggacgtc gcccgcgacg accgtgccga aggcatccgc
     5881 cgacaggcgt tcgccgccgc ggatcagctc gacgagcggc atttcccgtc ccagcgtccg
     5941 gcgcagcccc gagcggtcgc ggccgatcag cttgccgttc ctgccctccg ccacgaccct
     6001 cgcgtcgaac tcgcgtgcgt gctcctgggg ctcgccctgc ttggtgctga gcagccgagg
     6061 cgccaccagc cacaggtaag ggagggcaac gagcaacgcg atgctcgtcg tggcggtgaa
     6121 gtcgaacacc cccatcggtc gcatgccgaa gtcggcggcg atggacagga cgagcaggtt
     6181 ggtcgacgtg ccgatgctgg tcagcatgcc gccgatgagg atcgcgaaat tcaccggcat
     6241 cagggtcctg gaggacggat aacccgtgcg atcggccagg ctgagcagca tgggcagcat
     6301 catcaccagg accggcgtgt cgttgatgaa cccgctcacg aacgcggcga gggtgagggt
     6361 cagcagcaga ccgatcgcgg tgttgaactg ccacactctc gcgatcagcc gcacgatcgg
     6421 ctcgagcgcg cccgtcatcg tcaagccccg gccgaggacc atcaacgaac agatcgccac
     6481 gagcgccggg tggccgaagg cctggagcac ctcggcctcc gtgatccggt ggtcgggtga
     6541 aacgtaaggg aacaggtaga agatcgaaag cagcagcgcc agcagcagca gtgagaccag
     6601 ctccatccgg atccacctgc gcgtgtacag gtagaacacg gcgatcgtca cgatcagcac
     6661 ggccacggcg tgagggctag gcaaactgga ctcccttgag ttctggcgaa tgttgccaga
     6721 agcgaccacc tcgcgtcgtc gccgaagtgt gcatgtgccg actgacctag tgccgggtca
     6781 gcggatctgc acgtgtcttt gggaacgact cgcctgtgca cgcccctggg cgagcgctag
     6841 ggttggaccg gggtccgacg cggacccggc gcgcacagga gcaggcgatg caggcaacgc
     6901 agaagcacgc agggaaagct ggcatggggt gggggcgctt tgccgcgatg atcgccacgt
     6961 cgacggtgat catgtttccg ttgatgtacc agctcgtata tcgcgcagat catttgacgt
     7021 tcagcgtcac ccggctgctc tccgcgctga tcatgggcgc ggtcatgacg atcgtcatgc
     7081 tgggcttcat gtggtcgatg tacgaaggca cccggaccaa gatcgccgtc atggccggcg
     7141 cggccttgct tggctgcgtg ctgttcctgc tcaatcggag ccaggcgctg atcgacgaca
     7201 ctgcattcat gcaggcgatg attccgcatc attcgatcgc catcaacaat gcccgcaagg
     7261 cgcgcatcca cgatccgcgc gtgcgggaac tggccgatca gatcatctcg tcgcaggtac
     7321 gtgagatccg cgaaatggag ctgctggtcg aggacatcga acgcgacggc aggctggggg
     7381 aacgcgtgct gccggcccgc gcggccgtga tcacgccgga gatggagcga gaggtccagg
     7441 ccgccgtccg acgcgactga ggtcgcaggg cgctgcgctc gacgcggggc gcccatacag
     7501 aatgtaggcc atcgcgtacg gccatccgag ccaatgccga ggaaccgcgc gccgcgccag
     7561 ccggtcgcac ataggtactc cgttcgcgac gatacgaaga tgcacgacac tccccgacgc
     7621 tgggtctggg cggggcttgc gtcggccgcc ttcgtccccg gcgctcacgc cggcatcgag
     7681 gtcgaaatcg acgggctcga cgacgacgag atccgtggtg cggtccgggc ggccctgact
     7741 ttgacgcaat acaagcgacg cgacgtgacg ctggcgcgcg cgcgctacct gtacggacag
     7801 gcggaggagg agatcaagcg cgcgctggag ccgttcggct actacgaccc tggcgtcgag
     7861 ggccggttcg agacgatcga ggccggcgag taccgcgtga ccttttccgt caggcccggg
     7921 ccggccgtcc aggtcacccg ccgctccctc gagttgcccg aagacgtgcg caagctcgag
     7981 cccgtcgagg cggccctgat cgagttcgag ccggaacgcg gcgagcggct cgaccaccag
     8041 ctctacgagc gcagcaagca acgcgtcacg acggctctgc gtgccgtcgg ttacctggat
     8101 gccgaaacaa gcgtgcatca ggtggcggta aacacggcgc agcgcaccgc ggaaatcgac
     8161 ctcgcgtggg agctcggtcc ccgttaccgg ctcggaacgg tgcagttctc ggattcgcag
     8221 ttcgacgacc gtttcctgtc gggctacgta ccgtggcagc cgcaggacta ctactcgaca
     8281 gaggcgcttc tcacgctgca gcagcgactc agtcagacgg gttatttcca gaccgtggcc
     8341 gtcgagcccg tgctggacga gcgcggtgac ggcgtggtcc ctgtgcaggt cgtgctgacc
     8401 gagaacgagc gcaacctcta ccgggcgggg gtgtactaca gcaccgatta tggcgtcggc
     8461 ggccggctcg gctacgagcg gcgctggctc aaccgccgcg gtcacacgct cgacaccaac
     8521 gccgagtact cgcagcggct gcaggattac agcgtgacgt atgaaatccc gcgtcccagt
     8581 cccgaggaca agcggtacac gttcggcgcg gcctatcgcg acgaaacctc cgacaccgcg
     8641 cagtcacgga cgctgcggct cgcggccgcg gattcgcgga aggaatggaa gggattcgcg
     8701 cgcacgctcg gcctgcacta cctcgacggc gatttcgaag tcggcggcga gcgcggccgt
     8761 tcggccctgc tgttcttcga aggcacgctc gggcgcaagc actacgacga cctcgctttt
     8821 ccgcgggacg gttattcgtt cgacatcggc ctgaggcttg cgccgggcag cggcctgagc
     8881 gacacgagcc tcgcgcaggc ctgggtacag cacaagcggc tgctgcgcca gggcagtgac
     8941 cattcgcgaa tcatcctgcg cgcacgcctc ggcgccatgg cggtcggcga cttcgacgaa
     9001 ctgccaccgg acctgcggtt cttcgcgggt ggcgatcgct cgatacgagg gttcgattac
     9061 caggccatcg gcgaactgaa cgaccagggc aaggtcgtgg gcggccagta cctcgccgtg
     9121 gcgagtgccg agtacgagta ctacctgccc cagcgcgaga actggggggc ggccgtgttc
     9181 accgacatcg gcgatgcgtt cacgcacgac ttccgcgaga acgtctcggt cggcgtcgga
     9241 ctgcgctggc gttcgcccat cggcatggtg cgcgtcgacg tggccacgcc cgtcgagagc
     9301 cgtttcgacg acggcgtccg gctgcacatc acgatcgggc cggacctgtg acggacacgc
     9361 gcggaccgcc gagcgacacg ccagggcagg acggcgagcg cgcgtcccgg gcgacgcatc
     9421 ggggccggcg catcgccggc tacgtcctgc tcgcggtcgt cgcgctgctc gtgctgattg
     9481 cggccgtggt cgcctggatc gtgctcacgc cgggcggcac gcgctgggcc gtgggccagg
     9541 ccgagtcgcg gctgcctgca ctccggatcg agcacgtcga gggcacggcc ggacacgggt
     9601 tgacgctgcg gggtctccgg tacgagccgg aacagggcac gcgcgtgagg gccgcgctcg
     9661 cgcgcatcca actggacctg ggcgcgctgt ggaagcgctc ggtcgtcatc gaggacctct
     9721 tcctgcaggg tgcccgcgtg cagatcgcgg agtcgcccga ccaggaaccg caggagccgt
     9781 tttcgctcga gccgccggtg gacgtgatcg tggcccgtgg cagggcggaa gacgtgatcg
     9841 tcgccgacga aacgggcgcg atcgcgacga tccgtcacgc ggaactgcgg ggcggctgga
     9901 cgcgcgcgaa cggcatcgag gtcgagcagt tgcgcgtcgc ggcacgcgag ggagacgttc
     9961 ggctgcaggg ccatctggac ggtggccccg ggcagtaccg tgccgacggc gacgcaagcg
    10021 tgcgctggca ggtcggggcc cgaacttacg ctggcacggc acatgtttcc acggacgacg
    10081 cgcgcatccg cgtcgaaaca cgtctgacgt cgccgctcgc cgcgcgcgcg acgggctcgt
    10141 tcgaccagca ggcagggcag tgggccgcgg gcctgcgcgt accagcggtc gaccttcgcg
    10201 ccgagatggc cccggacgcg ccggtgggcg tggtgtcggc cgagctgcac gccaacggca
    10261 ccgaacgtac cgccgccgtg cagggcgaac tggtcgtgga cggtgagacc gtgcacgtcg
    10321 tcgacggtcg actcgtgctg cggccggagc ctcgaacgct gcacctcgag cgcctcgtgc
    10381 tgcggctgaa cgaggacccc ggccagttcg tggcgtctgg cactttcgct ctcgatgccg
    10441 accgcgtcac ggcggacctc aagacgcact ggcgcgagct cacgattccg gaggcctggg
    10501 caggacagca acttcacacc aacggccagc tggccgtgcg tggcagtgcc gagcgctatg
    10561 cgttgagcgg agaaatcgag gtcggcccgc ccgggcgatt ggccgacgtc agcgtggtcg
    10621 cccaaggaac accccgccgg ctcgcgctcg agaaattcgt cgtggagcag gaggccg
//
LOCUS       DASPFR010000185         5045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_297350, whole genome shotgun sequence.
ACCESSION   DASPFR010000185 DASPFR010000000
VERSION     DASPFR010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297350"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VD737_04705"
     CDS             complement(<1..976)
                     /locus_tag="VD737_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXW09892.1"
                     /translation="MRLEAAFNQFVSVTAMNLRNLPSRLGTSLVAVIGIGGVVAVLVS
                     LLSMGEGFRAALDLSGRDDVAMILRGGSTDELSSSITREEFNVIALAPGIARDARGPI
                     VSGELYTIVDLPMRSTGTAANVPFRGVGDRAMELRPGFRIVAGRMFVPGRDEVIVGRG
                     VFAQFGEVDLGREVKWGSQSWRVVGVFAAGGSVSESEVWTDAVVLRGVYRRGNTVQIV
                     RAQLESPSAIERVKASLEHDPRVNVTVRSERAFYADQSRILLALIKYVGSTLAVLMGI
                     GAVFAALNTMYSAVSSRTREIATLRALGFGAAPVVTSVLLEAAMLGLLG"
     gene            complement(985..2154)
                     /locus_tag="VD737_04710"
     CDS             complement(985..2154)
                     /locus_tag="VD737_04710"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXW09893.1"
                     /translation="MIGAVGKYLPLLAASLRRKRLRTFFTIASIVVAFLLFGLAESMR
                     FALRSGVEVAGADRLLTMHKVSFTQLLPASYENRIKAVDGVVAITPQTWFGAWFQDER
                     NQIPTFPTKPEAFLRIYPEIQVPEAEEQAWIADRAGILVGRSIATLFGWKVGDTVPLK
                     SAIWRREDGSDTWDVTVRAIFDMAQGGDTRQIVMHQEYFEEAKQQAKGLIGWYVIRVD
                     DVDHAQAVARQIDALFANSPAETKTSSERAMAQSFVNQIGNIGAILSAIVAAVFFTML
                     LVTANTMAQSVRERTNELGVLKTLGFSNPGVLGLVLGESMLVTTLGGVAGMVLAWYVG
                     VQFEPVLDQYLPGFRVPPSAFVQALAIMLALGAVAGAAPAWQAMRLRIVDALRRE"
     gene            complement(2151..2864)
                     /locus_tag="VD737_04715"
     CDS             complement(2151..2864)
                     /locus_tag="VD737_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXW09894.1"
                     /translation="MNATQAADVTSRPIVRIRDLVKEYRRGSEVVRVLDGLSLDIAQG
                     DFVALMGPSGSGKSTLLNLIGGLDRPTSGSLEVEGLRVDALTDASLTRWRADHVGFVF
                     QMYNLLPVLTAERNVELPLLLTGLGREQRMKRVAAALALVGLTERARHRPRELSGGQE
                     QRVGIARAIVTDPTLLLCDEPTGDLDRKSGDDVLTLLEALNDERGKTIIMVTHDPHAA
                     ERAHRIIHLDKGLLSEGHP"
     gene            complement(2861..4084)
                     /locus_tag="VD737_04720"
     CDS             complement(2861..4084)
                     /locus_tag="VD737_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXW09895.1"
                     /translation="MPLDKAALESLRLERDTDDNRYRERGGTQRWRWIAAAIVAVALV
                     LFAWRSFNGALPVDTITVDAAAANGAVLNASGYVVARRLATVSSKVTGRVSEVLFEEG
                     AEVEANQVLARLDRTTVQAEYAVAATSLEAARRNLREIEVRLADARRTLERNRSLVDR
                     KLVSQSVVDASQAEAAALAARLEAAKAEVDVANARVALSRQALDDLEIRAPFRGVVIS
                     KDAQPGEMVSPISAGGGYTRTGIATLVDMDSREVEVDVNEAYINRVRAGQRVEGVLDA
                     YPDWRIPAHVISIVPTADRQKATVRVRIGLDELDPRILPDMGIKVSFLEDATAAAAAP
                     QVRVPANAIVDEDGASHVWVVRDGKVERRAVKASPTPDGQVAVLEGLDGGETLVVDAP
                     ARLSEGRAVELKAGP"
     gene            4133..4948
                     /locus_tag="VD737_04725"
     CDS             4133..4948
                     /locus_tag="VD737_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HXW09896.1"
                     /translation="MTPTPRLVDIGLNLAHDSFEHDRDHVVATARAAGVEHMVITGST
                     LDSTAAAIELVRAAPETFRCTAGVHPHHAAEMRAEDVPRLRELLASPEVGAAGECGLD
                     HFRNFSPHEDQERAFRWQLELAIECGKPVFLHQRDAHDAFVSILDDYLPRLPGAVAHC
                     FTGEGRELRDYLDRGLSIGITGWICDERRGQHLRELVRLIPLDRLMAETDAPYLLPRD
                     LSPKPAHRRNEPKYLPHVVKTIAACRGEPYATIAAATTRNALEFFGFGGSASG"
BASE COUNT          796 a         1856 c         1652 g          741 t
ORIGIN      
        1 cgcccagcag tccgagcatc gccgcttcga gcagcaccga cgtgacgacg ggggccgcac
       61 cgaagccaag cgcgcgcagc gtggcgatct cgcgcgtgcg cgacgatacc gccgagtaca
      121 tcgtgttgag cgccgcgaac accgcgccga tgcccatcag caccgcgagc gtgctgccca
      181 cgtacttgat caacgcgagc aggatccgcg actggtccgc gtagaacgcc cgctcgctgc
      241 gcaccgtcac gttgacgcgg gggtcgtgtt cgaggctcgc cttgacgcgc tcgatcgcgc
      301 tcggcgattc gagctgggcg cgcacgatct ggacggtgtt gccccgtcgg tagaccccgc
      361 gcagcacgac ggcgtcggtc caaacttcgg actccgacac gctgcccccc gccgcgaaca
      421 cgccgacgac gcgccacgac tgcgagcccc acttgacctc gcgtccgagg tcgacctcgc
      481 cgaactgcgc gaacacgccg cgcccgacga tcacctcgtc gcgcccgggc acgaacatgc
      541 gtccagccac gatccggaag ccgggacgca gttccatcgc gcgatcgccc acgccacgga
      601 acggcacgtt ggcagcggtg cccgtgctgc gcatcggcag gtcgacgatc gtgtagagct
      661 cgccggagac gatcggcccg cgcgcgtccc gggcgatgcc cggcgcgagc gcgatcacgt
      721 tgaattcttc gcgggtgatc gagctcgaca gttcgtccgt cgacccgcca cgcaggatca
      781 tcgccacgtc gtcgcgaccc gacaggtcga gcgccgcgcg gaagccctcc cccatcgaca
      841 gcagcgacac cagcaccgcg acgacgccgc cgatgccgat caccgcgacc agcgacgtgc
      901 cgagccgcga cggcaggttg cgcaggttca tggccgtcac ggacacgaac tggttgaagg
      961 cggcctcgag gcgcactgcc gctcctactc gcgacgcagc gcgtcgacga tgcgcaaccg
     1021 catcgcctgc caggccggcg ccgcgccggc caccgcgccg agcgcgagca tgatcgcgag
     1081 cgcttgcacg aacgccgacg gcggcacccg aaacccgggc aggtactggt cgagcaccgg
     1141 ctcgaactgg acgccgacgt accaggcaag caccatgccc gcgacaccgc cgagcgtcgt
     1201 caccagcatc gactcaccga gcacgagccc gagcacgccc ggattgctga atcccagggt
     1261 cttgagcacg cccagctcgt tggtgcgttc gcgcaccgac tgcgccatgg tgttggccgt
     1321 gaccagcagc atcgtgaaga acaccgcggc cacgatcgcc gacaggatcg cgccgatatt
     1381 gccgatctgg ttcacgaacg actgggccat cgcgcgctcg gacgacgtct tggtctcggc
     1441 cggcgaattg gcgaacagcg catcgatctg gcgagccacg gcctgcgcat gatcgacgtc
     1501 gtcgacccgg atcacgtacc agccgatcaa ccctttcgcc tgctgcttcg cttcctcgaa
     1561 atactcctgg tgcatgacga tctggcgcgt gtcgccgccc tgcgccatgt cgaagatggc
     1621 ccgcacggtg acgtcccagg tatcggaacc gtcttcacgg cgccagatgg cggacttgag
     1681 cgggaccgtg tcgcccacct tccagccgaa cagcgtcgcg atgctgcggc cgacgaggat
     1741 cccggcgcgg tcggcgatcc acgcctgctc ctcggcctcg ggcacctgga tctcggggta
     1801 gatgcgcagg aacgcctcgg gcttggtggg gaacgtcggg atctggttgc gctcgtcctg
     1861 gaaccaggcg ccgaaccacg tctgcggcgt gatcgccacg acgccgtcga ccgccttgat
     1921 ccgattctcg tagctcgccg gcagcagctg cgtgaacgac accttgtgca tggtcaggag
     1981 ccggtcggcg cccgcgacct cgacgcccga acgcaacgcg aaccgcatcg actcggccag
     2041 cccgaacagc aggaacgcga ccacgatcga ggcgatcgtg aagaacgtgc gcagccgctt
     2101 gcgacgcagg ctcgcggcga gcagcggcag gtacttgccg acggctccga tcatgggtgc
     2161 ccttcgctca gcagtccctt gtcgaggtgg atgatccggt gcgcgcgctc ggcggcatgg
     2221 ggatcgtgcg tgaccatgat gatcgtcttg ccgcgctcgt cgttcagcgc ctcgagcagc
     2281 gtcagcacgt cgtcgccgga cttgcggtcg aggtcgcccg tcggctcgtc gcagagcagc
     2341 agcgtgggat cggtgacgat cgcgcgggcg atgccgacgc gctgctcctg cccgccggag
     2401 agctcgcggg gtcgatgacg ggcccgctcc gtcaggccca cgagggccag ggccgcggcg
     2461 acccgcttca tgcgctgctc gcggcccagc ccggtcagca gcagcggcag ctcgacgttg
     2521 cgctcggcgg tgagcacggg cagcaggttg tacatctgga acacgaagcc gacgtggtcg
     2581 gcgcgccagc gggtgagtga cgcgtcggtc agcgcgtcga cccgcagacc ctcgacttcc
     2641 agggagcccg aggtcggacg gtcgagcccc ccgatcaggt tcaggagcgt ggacttgccc
     2701 gagcccgagg ggcccatcag cgccacgaaa tcgccctgcg cgatgtccag cgacaggccg
     2761 tccagcacgc ggaccacctc cgagccgcgc ctgtattcct tgacgagatc gcggatgcga
     2821 acgatgggcc gcgacgtcac gtccgctgcc tgcgtcgcgt tcatgggccg gccttgagct
     2881 ccactgcgcg gccctcgctc aatcgcgccg gcgcatcgac cacgagcgtc tcgccgccgt
     2941 cgagcccctc gagcacggca acctggccgt cgggcgtcgg cgaggccttc accgcacgcc
     3001 gctcgacctt gccgtcgcgc accacccaga cgtggctcgc gccgtcctcg tcgacgatcg
     3061 cgttggccgg cacgcggacc tgcggcgcag ccgcggcggc ggtcgcgtcc tcgaggaagc
     3121 tcaccttgat gcccatgtcg ggcaggatgc gcgggtccag ttcgtccagt ccgatgcgca
     3181 cgcggacggt cgccttctgg cgatcggccg tcggcacgat gctgatcacg tgcgcgggga
     3241 tgcgccagtc cggataggcg tcgagcacgc cctccacgcg ctgcccggct cgcacgcggt
     3301 tgatgtaggc ctcgttgacg tccacttcga cttcgcgcga gtccatgtcg accagcgtcg
     3361 cgatgcccgt gcgggtgtag ccgccgcccg cggagatcgg cgagaccatt tcgccgggtt
     3421 gtgcatcctt cgagatcacc acgcccctga acggcgcgcg gatttcgaga tcgtcgagcg
     3481 cctgccggct cagcgccacg cgggcgttgg cgacgtcgac ctccgccttc gccgcctcga
     3541 gccgcgccgc gagcgcggcg gcctcggcct gggaggcgtc caccacgctc tgcgagacga
     3601 gcttgcgatc gaccagcgag cgattgcgct cgagcgtacg gcgcgcgtcg gccagccgca
     3661 cctcgatctc gcgcaggttg cgccgtgccg cctcgaggct ggtcgccgcc acggcgtact
     3721 cggcctgtac cgtggtgcgg tcgagccgcg cgagcacctg gttcgcctcg acctcggcgc
     3781 cctcctcgaa cagcacctcg ctcacgcgac cggtcacctt ggagctgacg gtcgcgaggc
     3841 gccgggcgac gacgtaaccc gacgcgttca gcaccgcgcc gttggcggcc gctgcatcca
     3901 ccgtgatcgt gtcgacgggc aacgcaccgt tgaacgagcg ccatgcgaac agcacgagcg
     3961 cgaccgccac gatcgccgcc gcgatccagc gccaccgttg agtaccgccg cgctcacgat
     4021 accggttgtc gtcggtatcg cgctccagcc gcaggctctc gagggctgcc ttgtccagtg
     4081 gcatggtgaa tcgctaggct cggaaagccg cgcacgttac cacagacact gcatgacccc
     4141 gactccccgc ctcgtcgaca tcggcctgaa cctcgcgcac gacagcttcg aacacgatcg
     4201 cgaccacgtg gtcgcgacgg cgcgtgccgc gggcgtcgag cacatggtga tcaccggcag
     4261 cacgctcgac agcacggcgg ccgcgatcga gctcgtccgc gccgccccgg agacgtttcg
     4321 ctgcaccgct ggcgtgcacc cgcaccacgc cgccgagatg cgggccgagg acgtgccgcg
     4381 cctgcgcgaa ctgctcgcca gtcccgaggt cggcgcggcc ggcgagtgcg gcctcgatca
     4441 tttccgcaac ttctccccgc acgaagacca ggagcgcgcc ttccgctggc agctcgagct
     4501 ggcgatcgag tgcggcaagc ccgtcttcct gcaccagcgc gacgcgcacg acgccttcgt
     4561 gtccatcctc gatgactacc tgccgcgcct gccgggtgcc gtggcgcact gcttcaccgg
     4621 cgagggccgc gagctccgcg actacctcga ccgtggtctt tcgatcggca tcaccggctg
     4681 gatctgcgac gagcgccgcg ggcagcacct gagggaactc gtccgcctga tcccactgga
     4741 tcggctgatg gccgagaccg acgcgccgta cctgctgccg cgcgacctga gcccgaagcc
     4801 cgcccatcgc cgcaacgagc ccaagtacct gccgcacgtc gtgaagacca tcgcggcctg
     4861 ccgcggcgag ccctacgcga ccatcgcggc cgcgacgacc cgcaatgcgt tagagttctt
     4921 cggcttcggc ggctccgcca gtggctagtg gtcagtggct agtggctagt agaaccacgt
     4981 ccgggttcac tagccactag ccactagcca cttgacttca caaaatcacg ctaaaagttt
     5041 gaaac
//
LOCUS       DASPFR010000186        11359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_786211, whole genome shotgun sequence.
ACCESSION   DASPFR010000186 DASPFR010000000
VERSION     DASPFR010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11359
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786211"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VD737_04730"
     CDS             <1..271
                     /locus_tag="VD737_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HXW09897.1"
                     /translation="QLRRAAVSVPSNVAEGSARRTTRDLTAFLHVARGSIAELETQLL
                     LAREVGYLDEAALNSVLPNLDEVGRLLNGLITSLRRRSRPTTAGH"
     gene            complement(289..1083)
                     /locus_tag="VD737_04735"
     CDS             complement(289..1083)
                     /locus_tag="VD737_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009390813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HXW09898.1"
                     /translation="MGFLAGKRALIVGLASERSIAHGIAAAMHREGAELAFTYQNDRL
                     KDRVVEMAAAFDSRATVPMDVASDAEIESAFAAVREAWDHLDIVVHSVAFAPREELAG
                     TFVESTTREGFKIAHEISSYSLTALAKAARPLMRGRAGAIITLSYLGAVRSIPNYNVM
                     GLAKASLEANVRFLAADLGPENIRVNAISAGPIKTLAAAGVSGFRKMLGQVAAATPLR
                     RNVTTDEVGNAAAFLCSDLASGITGEVLYVDAGYSHVGMSFPGESG"
     gene            complement(1088..2755)
                     /locus_tag="VD737_04740"
     CDS             complement(1088..2755)
                     /locus_tag="VD737_04740"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXW09899.1"
                     /translation="MKNNLRQRLRLACPTLIAVAALAGCASTDSTYSSLPPAPQHPGE
                     RATLDRETVTIPGTLMPSRTVPHLPSEQYTDLLDRIRDGYALPDVQHFAVDRELEHYR
                     SRPDFLDRTFKRGARYLYHIVAEIEKRNLPLELALLPVVESAFNPVAYSRSRASGLWQ
                     FIPSSGKHYGLEQNWWIDERRDVLEATDAALTYLQYLHGYFGGDWFLAIAAYNGGEGT
                     VRAAVRRNEQRGLPTDLWSLELRAETRDYVPKLLAISRIVRDPEAYGLQFAAIPNQPY
                     FEVVEPGRQVHLGDAAELAGITRDDMFALNPGYNRMTTPPKGPHRLLLPIPNAQQFRQ
                     AMLDHTLLEQQGKVLVAAVEPPPDVHHRVRRGDTLSSIARRYGVPTESLRAANDLRGS
                     VIHPGQSLLIPPTATGAAATLAALAPPREDIAAQLPERQKSSYSSPKPRVHVVRSGDT
                     LWGVARKYGVTVPTLAAANGLSSQAGLIPGARLDIPGSGGGSNARSNADRMTYKVRHG
                     DTLSQIADRYNVTVRQLMSWNQLRQASSLRAGQRLVLYVDPSRRSGG"
     gene            complement(2762..3538)
                     /gene="gloB"
                     /locus_tag="VD737_04745"
     CDS             complement(2762..3538)
                     /gene="gloB"
                     /locus_tag="VD737_04745"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HXW09900.1"
                     /translation="MLQVSPVRAFSDNYIWLIRAPADPGAAVVVDPGDDRPVEQALQQ
                     SGLRLGAILVTHHHADHVGGVGALAARHGASVYGPARERMPCDVQALDDGGTADLGQL
                     GLEFAVMAVPGHTLGHIAYVGHGALFCGDTLFSAGCGRLFEGTAAQMLDSLDRIAELP
                     DATRVYCAHEYTLSNLRFASAVEPDNSDVREALAETGRKRARDEVTLPSTLGRERRIN
                     PFLRSREGPVRAAAERHAGRALATDTEVFAAVRSWKDGFR"
     gene            3678..4406
                     /locus_tag="VD737_04750"
     CDS             3678..4406
                     /locus_tag="VD737_04750"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXW09901.1"
                     /translation="MDLAAAADRWLDSPLGAAVLDLETRLLQQELSDVVGFELLQVGR
                     WGDAAALCSGARTQHRTLIAPDATGPHSIRARFDTLPIASSSVEAVLLPHTLEHAQRP
                     HQLLREVDRVLVGEGNVIICAFSPWGPWGVRNFVAHGNFPPHAARMMSERRTRDWLSL
                     LGFEIVGARRYLFAPPWTQRMVGYRADSWLEREGPLIAAPLAGAYLLKAKKRVHAVTP
                     IRPAWVARPVMVGGAPEPTTRQAA"
     gene            4403..4888
                     /gene="rnhA"
                     /locus_tag="VD737_04755"
     CDS             4403..4888
                     /gene="rnhA"
                     /locus_tag="VD737_04755"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003180633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HXW09902.1"
                     /translation="MSSGQEDARALRAGDVIVYTDGACKGNPGPGGWGALLIAGEHRK
                     ELYGGESPTTNNRMELTAAIEALAALKRPCRVQLYTDSQYVRLGITEWLPQWKRRGWK
                     TADKKPVKNDDLWKQLESQIERHEVHWHWVRGHAGDPGNERADELANLGIMEKRLADG
                     G"
     gene            5016..6131
                     /locus_tag="VD737_04760"
     CDS             5016..6131
                     /locus_tag="VD737_04760"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXW09903.1"
                     /translation="MDFIDLKTQYRRLKPSIDARIARVLEHGQYVMGPEVAELEQKLA
                     EYVDARHCIGVASGTDALLIALMALDIGPGDEVITVPFTFFATAEMIALIGAKPVFVD
                     IDRGSYNMDPGKLEAAITPRTRAIMPVSLYGQCPDLDAIEAIARAHDVPVIEDAAQSF
                     GATYKGRRSGGLTAIGATSFFPSKPLGCYGDGGALFTNDDDLARVMREIRVHGQDRRY
                     HHPRLGLTGRLDSIQAAVLLAKLETFDEEVAARRRLGERYAELIRIKFGTGPQARVHA
                     PEVESYNTCVFAQYTVEVEHREIVETRMKQLDVPTAVHYPLSLHMQPVFKDLGYQEGD
                     FPNSEAAARRVISLPMHPYLSDAQQVEVVDALAQAVA"
     gene            6258..7025
                     /gene="dnaQ"
                     /locus_tag="VD737_04765"
     CDS             6258..7025
                     /gene="dnaQ"
                     /locus_tag="VD737_04765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HXW09904.1"
                     /translation="MRQIVFDTETTGLEPELGHRIIEIGAIEIVNRRRTGRKFHRYLC
                     PDREIDSGAVQVHGLTSEFLRGQPRFADVFDELREFLAGAELIIHNAAFDVAFLDAEM
                     KALVAQRPDLGGLCTRDVCGVLDTLALARQMHPGQRNSLDALCKRYSVDNSHRDLHGA
                     LLDADLLLEVYLAMTGGQGALTLDDHVTSAHVATAAARTVRPAGMLVVLRANEQEIEA
                     HEALLRLLDKASGGRTAWRSFEPAPASTPMAAAEATA"
     gene            7022..8536
                     /gene="nhaB"
                     /locus_tag="VD737_04770"
     CDS             7022..8536
                     /gene="nhaB"
                     /locus_tag="VD737_04770"
                     /inference="COORDINATES: protein motif:HMM:NF018188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton antiporter NhaB"
                     /protein_id="HXW09905.1"
                     /translation="MIYTLSQSFGRAFLGQSPGWYKYTIVAFLIANPILLLLVGPVLT
                     GWVVLGEFILTLAMALRCYPLQPGGLIAIEAALIGLTTPESIYEEVTTAFPVILLLIF
                     MVAGIYFLRDLLLYVFTRLILRVRSRATLALTFCAASALLSAFLDALTVVAVAVTVGM
                     GFYEVYHRVASGKAHQALHHDSATDEFVEVTHRDNLEGFRGFLRSMMMHGAVGTAIGG
                     VMTQVGEPQNLLIAEQLGWHFGEFFVAMAPVTLPVMLAGFATCWSVERLGLFGYGSPL
                     PDATRAVLEDFAREHAANATMRDRQILITQASVALVLVLSLAFHVAEVGLIGLMVIVL
                     ATTFTGITEEHRIGKAFESALPFTALLVVFFVIVAVIHDQHLFEPVISFVLSLEGQMQ
                     AVMLYLAAGVLSTISDNVFVASIYITEVKAAFLQGVVNRSQFEWLAVAINTGTNVPSV
                     ATPNGQAAFLFLLTSTLAPLIRLGYGRMVWMALPYFVTMTTVGLLALAAATSGL"
     gene            8574..9893
                     /gene="tviB"
                     /locus_tag="VD737_04775"
     CDS             8574..9893
                     /gene="tviB"
                     /locus_tag="VD737_04775"
                     /inference="COORDINATES: protein motif:HMM:NF011729.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HXW09906.1"
                     /translation="MTERNTKNSPGDMAKFNLRNLKVGIVGLGYVGLPLAVEFGKRFQ
                     TVGFDIKPDRVDELRRGVDSTLEVESKELAEATKLSFTTSLADLKPCRVYIVTVPTPI
                     DDYKRPDLTPLEKSSESIGKVLKKGDVVVYESTVYPGCTEEVCVPILERVSGLKFNKD
                     FYAGYSPERINPGDKEHRVTTIRKVTSGSTPAVAEFVDSLYRAIVRVGTHKASSIKVA
                     EAAKVIENTQRDVNIALINELALIFNRLGIDTEEVLLAAGSKWNFLPFRPGLVGGHCI
                     GVDPYYLTHKAQSIGYHPEMILAGRRINDNMGIYVAAKVTQLMIQRRIQVRDSRILIL
                     GLTFKENCPDVRNTKIVDVVRELEKYGAAVDVHDPWADADDAEHEYGLRPVRKLKPGT
                     YDAIVMGVAHQEFRQMGVEKIRALGRKNHVLYDIKYVFKAGEVDGRL"
     gene            10002..11045
                     /locus_tag="VD737_04780"
     CDS             10002..11045
                     /locus_tag="VD737_04780"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HXW09907.1"
                     /translation="MKILVTGAAGFIGYHTAKLLLERGDEVVGLDNLNDYYDVRLKLA
                     RLEQLQAYPRFRFVKLDLADRAGMTELFEREKFQRVIHLGAQAGVRYSIQNPLAYVDS
                     NVVGTANVLEGCRHHGVEHLVYASTSSVYGANTKMPFSVHQNVDHPLSFYAATKKANE
                     LMAHTYAHLYALPVTGLRFFTVYGPWGRPDMALFLFTRNILAGKPIDVFNYGHHRRDF
                     TYVGDIAQGVVRACDRVATPHAGWDSDAPDPATSKGPYRLYNIGNNKPVELLTYIRTL
                     EQCLGRKAEMNLLPLQAGDVPDTWADIDELTRDVGYTPATPVETGIRAFVDWYLEYYK
                     DGEPANPRVVNLG"
BASE COUNT         1853 a         3797 c         3858 g         1851 t
ORIGIN      
        1 tcaactgaga agggccgccg tctcggttcc ctccaatgtc gccgaaggtt cggcacgcag
       61 gactacgcgc gacctaacgg cttttttgca tgttgcgcgc gggtctatcg ccgaactcga
      121 aactcagctg cttcttgcgc gcgaggtcgg atatctcgac gaagctgcct tgaattcagt
      181 tttgccgaac ctggatgaag tcgggcggct cttgaacggg ctgatcacat cacttcggcg
      241 ccgatcgcga ccgacgacgg cgggccacta gccactagcc actagccact agccactttc
      301 ccccgggaag ctcattccca catgactgta ccccgcatcc acgtacagca cttcgccggt
      361 gatgcctgag gcgaggtcgg agcacaggaa agcggcggcg ttgccgactt cgtcggtcgt
      421 cacgttgcgg cgcagcggag tcgcggcggc cacctgtccc agcatcttgc ggaagccgct
      481 cactccggcg gcggcgagcg tcttgatggg cccggcggag atcgcgttga cgcggatgtt
      541 ctcagggccc aggtccgcgg ccaggaagcg cacgttggcc tcgaggctcg ccttggcgag
      601 ccccatcacg ttgtagttcg gaatcgagcg cacggccccc aggtacgaga gcgtgatgat
      661 cgcgccggcg cgcccgcgca tcagcggccg cgccgctttc gcgagcgccg tgaggctgta
      721 gctcgagatc tcgtgcgcga tcttgaagcc ttcgcgggtc gtgctctcga cgaacgtgcc
      781 ggcgagctcc tcacgcgggg cgaacgccac cgagtggacg acgatgtcca ggtggtccca
      841 ggcctcgcgc acggccgcga acgccgactc gatctcggcg tcggaggcga cgtccatcgg
      901 cacggtcgcc ctggaatcga acgcggccgc catctcgacg acgcggtcct tgaggcggtc
      961 gttctggtac gtgaacgcga gttcggcgcc ttcgcgatgc atcgcggcgg cgatgccgtg
     1021 ggcgatcgag cgctcggacg cgagccccac gatcagggcg cgcttgcctg ccaggaatcc
     1081 cacggtatca gcctccggaa cgacggctcg gatcgacgta tagcaccagg cgctgtccgg
     1141 cgcgcagcga ggatgcctgg cgcagctggt tccagctcat cagctgccgc acggtcacgt
     1201 tgtagcggtc ggcgatctgc gagagcgtgt cgccgtgccg gaccttgtac gtcatgcgat
     1261 cggcgttcga acgcgcgttg ctgccgccgc cgctgcccgg gatgtcgagg cgagccccgg
     1321 ggatcagtcc cgcctggctc gaaagtccgt tggcggcggc gagcgtcggc acggtcaccc
     1381 cgtacttgcg cgcgacgccc cagagcgtgt cgccggaacg cacgacgtgc acgcgtggtt
     1441 tgggcgagga ataggaactc ttctggcgtt cgggcaactg ggccgcgatg tcctcgcgcg
     1501 gcggggcgag tgccgcgagc gtggccgccg ccccggtggc cgtgggtgga atcaacagtg
     1561 actgtcccgg gtggatgacc gagccgcgca ggtcattggc ggcgcgcagg gactccgtcg
     1621 gcacgccata gcggcgcgcg atcgagctga gcgtgtcgcc gcgacgtaca cgatggtgca
     1681 cgtcgggcgg cggctcgacc gcggcgacga gcaccttgcc ctgctgctcg agcagcgtgt
     1741 ggtcgagcat cgcctgacgg aactgctgcg cgttcgggat cggcagcagc agccggtgcg
     1801 ggcccttggg cggggtggtc atgcggttgt agccggggtt caacgcgaac atgtcgtcgc
     1861 gcgtgatgcc cgcgagttcc gcggcgtcgc ccaggtgcac ctggcggccg ggttcgacga
     1921 cttcgaagta cggctggttc gggatggcgg cgaactgcag gccgtaggct tcggggtcgc
     1981 gcacgatgcg gctgatcgcg agcagcttcg gcacgtagtc gcgggtctcg gcgcgcagtt
     2041 ccaggctcca gaggtcggtc ggcagcccgc gctgctcgtt gcggcgcacc gccgcgcgca
     2101 cggtgccttc gccgccgttg taggcggcaa tggccaggaa ccagtcgcca ccgaagtaac
     2161 cgtgcaggta ctgcaggtac gtgagcgcgg cgtcggtcgc ctcgagcacg tcacgtcgct
     2221 cgtcgatcca ccagttctgc tcgagcccgt agtgcttgcc cgaggacgga atgaactgcc
     2281 agagccccga ggcgcggctg cgtgaatacg cgaccgggtt gaaggcgctc tcgacgaccg
     2341 gcagcagcgc gagttcgagt ggcaggttgc gtttttcgat ctcggcgacg atgtgataga
     2401 ggtagcgcgc tccgcgcttg aacgtgcggt cgaggaaatc cggccggctg cggtagtgct
     2461 cgagctcgcg gtcgaccgca aagtgctgca cgtcgggcaa cgcgtagccg tcccggatcc
     2521 ggtcgagcag gtcggtgtac tgctcggagg gcaggtgcgg cacggtgcgc gagggcatga
     2581 gcgtaccggg gatcgtcacc gtctcgcggt ccagcgtcgc acgctcgccg gggtgttgcg
     2641 gcgcgggcgg caggctcgaa tatgtcgaat ccgtcgaggc gcagcccgcc agcgccgcga
     2701 cggcgatcaa ggtgggacag gcgagtcgca gtcgttggcg gaggttgttc ttcatttcgc
     2761 ctcagcggaa gccgtccttc catgagcgca cggcggcgaa cacctcggtg tccgtcgcga
     2821 gcgcccggcc ggcgtgcctt tcggccgccg cacgcacggg accctcgcgc gagcgcagga
     2881 aggggttgat gcggcgctcc cgtcccagcg tggacggcag cgtgacttcg tcgcgcgcgc
     2941 gcttgcggcc ggtctcggcc agcgcctcgc ggacgtcgga gttgtcgggc tcgacggcgc
     3001 tggcgaaccg gaggttcgaa agcgtgtact catgggcgca gtacacccgg gtcgcgtcgg
     3061 gcaattctgc gatgcggtcg agggaatcga gcatttgcgc ggccgtgccc tcgaagaggc
     3121 gcccgcagcc ggcgctgaac agcgtgtcgc cgcagaacag ggcgccatgg cccacgtagg
     3181 cgatgtgacc cagcgtgtgt ccggggaccg ccatgacggc gaattcgagc cccagctgtc
     3241 caaggtctgc cgtgcctccg tcatcgagcg cctgcacgtc gcagggcatc ctctcccggg
     3301 ccggtccgta gaccgaggcc ccgtggcggg ctgccagcgc cccgaccccg ccgacgtggt
     3361 ccgcgtggtg atgcgtcacc aggatcgcgc ccagtcgcag cccggactgc tgcaaagcct
     3421 gctccacggg acggtcgtca cccgggtcta ctaccaccgc ggcgccgggg tctgccggcg
     3481 cacggatcag ccagatgtag ttgtccgaga acgcccgtac cgggctcacc tgcagcatgc
     3541 gctacgagac tcctcgttcg atccgcgggc caatgagtcc gggcggcggt attagagcgg
     3601 gtcgccacca aaatttccat aagcggacgc acgttttttc caacgcgggt aggcacttgc
     3661 cgttatcctt gcggcccatg gaccttgctg ccgccgccga ccgctggctc gactccccac
     3721 tgggcgcggc cgtgctcgac ctcgagacgc ggctgctcca gcaagagctc tccgacgtcg
     3781 tgggcttcga actgctgcag gtcggtcgct ggggcgacgc cgccgcatta tgttcaggcg
     3841 cgcgcacgca gcaccgcacg ctgatcgcgc ccgacgcgac ggggccgcac agcatccgcg
     3901 cgcggttcga caccctgccc atcgcgagca gttcggtcga ggccgtgctg ctgccgcaca
     3961 cgctcgagca cgcgcagcgg ccccaccagt tgttgcgcga ggtcgatcgg gtgctggtcg
     4021 gcgagggcaa cgtgatcatc tgcgcgttca gcccgtgggg accgtggggc gtgcggaatt
     4081 tcgtcgcgca cggcaacttc ccgccgcacg ccgcgcgcat gatgagcgag cgccgcacgc
     4141 gcgactggct gagcctgctc ggcttcgaga tcgtcggcgc acgtcgctac ctgttcgcgc
     4201 cgccgtggac ccagcgcatg gtcggctacc gcgccgacag ctggctcgag cgcgaagggc
     4261 cgttgatcgc ggccccgctc gccggtgcct acctcctcaa ggcgaagaaa cgcgtccacg
     4321 ccgtgacgcc gatccgcccg gcctgggtgg cgcgcccggt catggtcggc ggcgcacccg
     4381 agcccacgac gagacaggcg gcgtgagctc gggccaggag gacgcacgcg cgcttcgtgc
     4441 gggcgacgtg atcgtctaca cggacggtgc ctgcaagggc aaccccggcc cgggcgggtg
     4501 gggggcgctg ctgatcgcgg gcgagcatcg caaggaactc tacggcggcg agtcgccgac
     4561 gaccaacaac cgcatggagc tgacggcggc gatcgaggca ctggccgcgc tcaagcgtcc
     4621 ctgccgcgtg cagctctaca ccgactcgca gtacgtgcgc ctcggcatca ccgaatggct
     4681 gccgcagtgg aagcgtcgtg gctggaagac cgcggacaag aagcccgtca agaacgacga
     4741 cttgtggaag cagctggaaa gccagatcga gcgtcacgag gtccactggc actgggtgcg
     4801 cggccacgcc ggcgatcccg gcaacgaacg cgccgacgag ctggcgaacc tgggaatcat
     4861 ggagaagcgg ttagcggatg gcggttagcg gttagtcaag aaccatgacc tccgcggccc
     4921 acggatttgc tgacgacgcc caggaaagcg actctgacta accgctaacc gctatccgct
     4981 aaactacgaa gagcagtcgc ccgaacagca gcgccatgga cttcatcgac ctcaagaccc
     5041 agtacagacg cctgaagccg tcgatcgacg cgcgcatcgc cagggtgctg gagcacgggc
     5101 agtacgtcat gggtccggag gtggccgagc tcgagcagaa actcgcggag tacgtcgatg
     5161 ctcgccactg catcggcgtc gcgagcggca ccgatgcgct attgatcgcg ttgatggcgc
     5221 tcgacatcgg gccgggcgac gaggtgatca ccgtcccctt cacgttcttc gccaccgccg
     5281 agatgatcgc gctgatcggg gcgaagccag tcttcgtcga catcgaccgt ggcagctaca
     5341 acatggatcc cgggaagctc gaggcggcga tcacgcctcg cacccgcgcg atcatgccgg
     5401 tctcgctgta cgggcagtgc ccggacctgg acgcgatcga agcgatcgca cgcgcgcacg
     5461 acgtgccggt gatcgaggac gcggcgcaga gcttcggggc gacctacaag ggccggcgct
     5521 cgggaggcct cacggcgatc ggcgcgacct cgttcttccc ctccaagccg ctcggctgct
     5581 acggcgacgg cggcgcgctg ttcaccaacg acgacgacct ggcgcgcgtg atgcgcgaga
     5641 tccgcgtgca cggccaggac cgccggtatc atcatccgcg gctgggcctc acgggcaggc
     5701 tggactcgat ccaggccgcc gtgctgctcg cgaagctcga gaccttcgac gaggaggtcg
     5761 cggcgcggcg ccggctcggc gagcgttacg ccgagctgat ccgcatcaag ttcggcaccg
     5821 gcccacaggc ccgcgtgcac gcgcccgaag tggagtcgta caacacctgc gtgttcgcgc
     5881 agtacacggt cgaggtcgaa caccgcgaaa tcgtcgagac gcgcatgaag cagctcgacg
     5941 tgccgaccgc cgtgcactac ccgctgtcgc tgcacatgca gcccgtcttc aaggacctcg
     6001 ggtaccagga gggcgatttc ccgaactcgg aagccgccgc ccgccgcgtg atcagcctgc
     6061 cgatgcaccc gtacctgagc gatgcgcagc aggtcgaggt tgtggatgcg ctggctcagg
     6121 cggtcgcctg agccagcaaa gtgttcagtg tctagtgtct agtgtctagt gttcagtaag
     6181 agccgactgc gtcggcgaga gcgacgccgc ccttcttaac tagacactag acactaaccg
     6241 ctagacactt cacttccatg cgtcaaatcg tcttcgacac cgaaaccacg ggcctggagc
     6301 cggagctcgg tcatcggatc atcgagatcg gggcgatcga gatcgtcaac cgccgtcgca
     6361 cggggcggaa gttccaccgt tacctgtgtc cggaccgcga gatcgattcc ggggccgtgc
     6421 aggtgcacgg gctcaccagt gagttcctgc gcgggcagcc gcgtttcgcg gacgtcttcg
     6481 acgaattgcg cgagttcctc gccggcgcgg aactgatcat ccacaacgcg gcgttcgacg
     6541 tcgcgttcct cgacgcggag atgaaggcgc tcgtggcgca gcgcccggac ctcggcggcc
     6601 tctgtacgcg cgacgtctgc ggcgtactcg acacgctcgc gctggcgcgg cagatgcacc
     6661 cgggccagcg caacagcctc gacgcgctct gcaagcgcta ttcggtcgac aactcccacc
     6721 gcgacctgca cggcgcgctg ctggacgccg acctgctgct cgaggtctac ctggcgatga
     6781 cgggcgggca gggcgcgctc acgctcgacg accacgtcac ctcggcgcac gtcgcaaccg
     6841 ccgcggcccg cacggtccgg ccggccggca tgctggtcgt gctacgggcg aacgaacagg
     6901 agatcgaggc gcacgaggcg ctgctccggt tgctcgacaa ggccagcggc ggcaggaccg
     6961 cgtggcggtc gttcgagccg gcgccggcgt ccacgccgat ggccgccgcc gaagcgacgg
     7021 catgatctac acgctgtcgc agtcgttcgg ccgggcgttc ctgggccagt cgcccggctg
     7081 gtacaagtac acgatcgtcg ctttcctgat tgcgaacccg atcctgctgc tgctcgtcgg
     7141 gccggtgctg accggctggg tggtgctcgg cgagttcatc ctgacgctcg cgatggcgct
     7201 gcgctgttat ccgctgcagc cgggcggcct gatcgccatc gaggccgcgc tgatcgggct
     7261 cacgacgccc gagagcatct acgaggaggt gacgacggcg ttccccgtga tcctgctgct
     7321 gatcttcatg gtggcgggca tctacttcct gcgcgacctg ctgctgtacg tgttcacgcg
     7381 cctgatcctc cgcgtgcgca gccgcgccac gctggcgctc acgttttgcg cggcgtcggc
     7441 cttgctgtcg gcgttcctcg acgcgctgac ggtcgtggcg gtcgcggtca ccgtcggcat
     7501 gggcttctac gaggtctacc accgcgtggc ttcgggcaag gcgcaccagg cactgcatca
     7561 cgactcggcc accgacgagt tcgtcgaggt gacgcatcgc gacaacctcg aggggtttcg
     7621 gggcttcctg cgctcgatga tgatgcacgg cgccgtcggc accgcgatcg gcggcgtgat
     7681 gacgcaggtg ggcgagccgc agaacctcct gatcgcggag cagctcgggt ggcacttcgg
     7741 cgagttcttc gtggcgatgg cacccgtgac gctgcccgtg atgctggccg gcttcgcgac
     7801 gtgctggtcg gtggaacgcc tcgggctgtt cggatacggc tcaccgctgc cggacgcgac
     7861 gcgtgccgtg ctcgaggatt tcgcgcgcga gcacgccgcc aacgcgacca tgcgcgatcg
     7921 acagatcctg atcacccagg cgtccgtcgc gctggtgctg gtgctgtcgc tggcgttcca
     7981 cgtggccgag gtcgggctga tcggcctgat ggtcatcgtc ctcgccacga cgttcacggg
     8041 aatcaccgag gagcaccgca tcggcaaggc cttcgagtcg gccctgccgt tcacggcgct
     8101 gctggtcgtg ttcttcgtga tcgtcgcggt catccacgac cagcacctgt tcgagccggt
     8161 gatctcgttc gtgctgtcgc tcgagggcca gatgcaggcc gtcatgctgt acctggccgc
     8221 cggcgtgctc tccacgatca gcgacaacgt gttcgtcgcc agcatctaca ttaccgaggt
     8281 caaggccgcg ttcctgcagg gcgtcgtgaa ccgaagccag ttcgagtggc ttgccgtcgc
     8341 gatcaacacg ggcaccaacg tgccgagcgt ggcgacgccg aacgggcagg ccgccttcct
     8401 gttcctgctg acgtccacgc tcgcgccgtt gatccgcctc ggctacggcc gcatggtctg
     8461 gatggccctg ccgtatttcg tcaccatgac cacggtcggc ctgctcgcac tcgccgcggc
     8521 caccagcggg ctatgacggg cgtctcaaat agcacgccag tttcgtggca tccttgaccg
     8581 agcggaacac caagaacagt cccggggaca tggccaagtt caacctgcgc aacctcaagg
     8641 tcgggatcgt cggtctcggc tacgtcggcc tgccgctcgc cgtcgagttc ggcaagcggt
     8701 tccagaccgt cggattcgac atcaagcccg accgcgtcga cgaactgcga cgcggtgtcg
     8761 acagcacgct cgaagtcgag tcgaaggagc tcgcggaagc gaccaagctc tcgttcacga
     8821 cgagcctggc agacctgaag ccgtgtcgcg tgtacatcgt cacggtgccc acgccgatcg
     8881 acgactacaa gcgccccgac ctcacgccgc tcgagaagtc gagcgagtcg atcggcaagg
     8941 tgctcaagaa gggcgacgtc gtggtctacg agtccacggt gtacccgggc tgcacagagg
     9001 aagtgtgcgt cccgatcctc gagcgcgtgt cggggctcaa gttcaacaag gacttctacg
     9061 ccggttacag tccggagcgc atcaacccgg gggacaagga gcaccgcgtc acgaccatcc
     9121 gcaaggtcac ctcgggctcg acaccggcgg tggccgagtt cgtcgattcg ctgtatcgcg
     9181 cgatcgtgcg cgtcggtacg cacaaggcgt cgagcatcaa ggtggccgag gccgccaagg
     9241 tcatcgagaa cacgcaacgc gacgtcaata tcgcgctgat caacgaactc gcgctgatct
     9301 tcaaccgcct cggcatcgac accgaggagg tgctgctcgc ggccggcagc aagtggaact
     9361 tcctgccgtt ccggcccggg ctggtcggcg ggcactgcat cggcgtggac ccgtactacc
     9421 tgacgcacaa ggcgcagtcg atcggctacc accccgagat gatcctcgcc gggcgccgca
     9481 tcaacgacaa catgggcatc tacgtcgccg ccaaggtgac gcagctcatg atccagcggc
     9541 gcatccaggt gcgcgactcg cggatcctga tcctggggct cacgttcaag gagaactgcc
     9601 ccgacgtccg caacaccaag atcgtcgacg tcgtgcgcga gctcgagaag tacggcgccg
     9661 ccgtcgacgt gcacgacccg tgggcggacg ccgacgacgc cgagcacgaa tacggcctgc
     9721 ggcccgtgcg taagctcaag cccggcacct acgacgcgat cgtcatgggc gtcgcgcacc
     9781 aggaattccg tcagatgggc gtcgagaaga tccgcgcgct cggcaggaag aaccacgtgc
     9841 tgtacgacat caagtacgtg ttcaaggccg gggaagtgga tggacgactg tgaagaagtg
     9901 gtcagtggct agtggctagt ggttagtggg cggagaaagc gcctcagcgt ccgccgcctt
     9961 ctcttacact agccactgac cactagccac tggccacttg catgaagatt ctcgtcactg
    10021 gcgccgcggg cttcatcggc taccacacgg cgaagctgct gctcgagcgt ggtgacgagg
    10081 tcgtcgggct cgacaacctc aacgactact acgacgtccg gctcaagctc gcgcgcctcg
    10141 agcagctgca ggcgtacccc cggtttcgct tcgtcaagct cgacctcgcg gatcgcgcag
    10201 ggatgacgga gctgttcgag cgcgagaagt tccagcgcgt gattcacctg ggtgcgcagg
    10261 ccggcgtgcg ttactcgatc cagaatccgc tcgcctacgt cgacagcaac gtcgtcggta
    10321 ccgccaacgt gctggaaggt tgtcgtcacc acggggtcga gcacctggtc tacgcctcga
    10381 ccagctccgt gtacggcgcc aacacgaaga tgccgttctc ggtgcaccag aacgtcgatc
    10441 atccgctctc gttctacgcg gcgaccaaga aggcgaacga gctgatggcg cacacctatg
    10501 cgcacctcta cgcactgccg gtgaccgggc tgcgattctt cacggtctac gggccgtggg
    10561 gacggcccga catggcgctg ttcctgttca cgcggaacat cctcgccggc aagccgatcg
    10621 acgtcttcaa ctacggacac caccgtcgcg acttcacgta cgtcggcgac atcgcgcaag
    10681 gcgtggtgcg ggcatgcgac cgcgtcgcga ccccgcacgc gggctgggac agcgacgcgc
    10741 cggaccccgc aacctcgaag gggccgtatc gcctctacaa catcggcaac aacaagccgg
    10801 tcgagctgct gacctacatc cgcacgctcg agcagtgcct gggccgcaag gccgagatga
    10861 acctgctgcc gctgcaggcg ggcgacgtgc cggacacgtg ggcggacatc gacgagctga
    10921 cgcgcgacgt gggctacacg ccggcgacgc cggtcgagac gggcatccgc gcgttcgtcg
    10981 attggtacct cgagtactac aaggacggcg aaccggccaa tccgagggtc gtgaacctcg
    11041 gatgatcgat ccctctcccg tgcagcggga gagggtgggg tgaggggacc gcgcgaaacg
    11101 cgggtgaggg cggatcacct ctcccgcgtc ggcgggagag gtcgcgcgaa gcgcgggtga
    11161 gggcccggct tccgtggcac ggccgccgac ggtgttaagc ttttccgcat gacttcaccc
    11221 cgcaacgagc tgccggcctg gaaatctctt gaggcgcacg ctgctgggcg cgtctgcacc
    11281 ctgcacctgc gtgacctgtt cgcctcggac ggctcgcgcc gcttcgacca gttgagtgtc
    11341 caggcgctcg acctgctct
//
LOCUS       DASPFR010000187         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1395164, whole genome shotgun sequence.
ACCESSION   DASPFR010000187 DASPFR010000000
VERSION     DASPFR010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395164"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VD737_04785"
     CDS             <1..774
                     /locus_tag="VD737_04785"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination factor protein RarA"
                     /protein_id="HXW09908.1"
                     /translation="EREYSTGASQVRAEDGAIATLARAADGDARRALNLLEIATDLAQ
                     AGDGVVTETIASEVSAGGYRRFDKKGEQFYDQISALHKAVRGTDPDAALYWLCRMLDG
                     GCDPRYVARRVLRMASEDVGNADPRALQLALQAAETYERLGSPEGELALAQAVVFMAC
                     AAKSNAVYVAFKAAMADAQAHGTLEVPLRLRNAPTGLMKGLGYGKGYRYAHDEPDAIA
                     AGETYFPDEMTPARYYEPVPRGLEIKIAEALERVRARSK"
     gene            1019..>2003
                     /locus_tag="VD737_04790"
     CDS             1019..>2003
                     /locus_tag="VD737_04790"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HXW09909.1"
                     /translation="MRKNHIRRRPVARLSVPARTLAGLLLATVGMLDAAHASSSAAKD
                     VQLRVLSSPANYVSGGDARIEVRAAPGLHDKLVLYVNGSRLDATWQKTGSHRVQAVID
                     GLQVGENTLEVYVKGQSLRDTLRLVNHPITGPMFSGEQQQPFVCTTIQSAVGRQPLVD
                     SATPPGYKVTDGTGATIGYSRDCSIETFVTYWYRRNTGGGLVPLPAGARPADMGTVQL
                     ADGRTVDFVVRREVGSINRFLYSIAMLAPAPGADNAQQNDTSLWNGKLLYWFQGGVAI
                     GHTQGTVHSGSMNPDVLGQGMAIVHSSGNNTGTHYNLEVAGETAMMTKERFI"
BASE COUNT          345 a          686 c          689 g          283 t
ORIGIN      
        1 gagcgcgagt attcgaccgg ggcctcgcag gtgcgcgcgg aggacggggc gatcgcgacg
       61 ctggcgcgcg cggcggacgg cgacgcgcgt cgtgcgctca acctgctcga gatcgcgaca
      121 gatctcgcgc aggcgggcga cggcgtcgtc accgagacca tcgcgtccga ggtctccgcg
      181 ggcggctacc ggcgtttcga caagaagggc gagcagttct acgaccagat ctcggcactg
      241 cacaaggccg tgcgcggcac cgatccggat gccgcgctgt actggctctg ccggatgctc
      301 gatggcggct gcgatccgcg ctacgtcgcg cgtcgcgtgc tccgcatggc gtccgaggac
      361 gtcggcaatg ccgacccgcg tgccttgcaa ctcgcccttc aggccgcgga aacctacgag
      421 cgcctcggca gccccgaggg cgagctcgcg ctcgcacagg ccgtcgtgtt catggcctgc
      481 gcggccaaga gcaacgccgt ctacgtcgcg ttcaaggcgg cgatggccga cgcacaggcg
      541 cacggcacgc tcgaggtgcc gctgcggctg cgcaatgcac cgaccggact gatgaaaggc
      601 ctcggttacg gcaagggcta tcgctacgcc cacgacgagc cggacgcgat cgccgccggc
      661 gaaacctact tcccggacga aatgactccc gcgcggtatt acgagcccgt gcctcgcgga
      721 ctggaaatca agatcgccga ggcgctcgag cgcgtcaggg cacgctccaa atagtcctcc
      781 cgcgatgcga gacacctccg caccgtcgtt caggcgggtg cgggcgtcgt cctgttgcga
      841 cgcaagacga gcggtccaac tgctcgacgc gcgccagcct ggatcgagcg ttccttgaga
      901 cttgcacaag cgaacggatt tagtcaccgt tgcggcagcg ccatcgacgg cccggaaggc
      961 cgcttgcgcc acaggcacat cgccgcgccc ggcgcggccc agatgaaaga ggggaatcat
     1021 gcgaaagaat cacatcaggc gacggcctgt cgcccggctg tccgtgccgg cacgcaccct
     1081 ggccggcctg ctgctggcga cggtcggtat gctcgatgcg gcccacgcct cgtcgagtgc
     1141 ggcgaaggac gtgcagctgc gcgtgctgtc gagcccggcc aattacgtct ccggtggcga
     1201 cgcgcgcatc gaggtccgcg ccgcaccggg tctgcacgac aagctcgtgc tgtacgtcaa
     1261 cggcagccgg ctcgacgcga cctggcagaa gaccggctcg caccgcgtgc aagccgtgat
     1321 cgacggcctg caggtcggcg agaacacgct cgaggtctac gtgaaggggc agtcgctgcg
     1381 cgacacgctc aggctcgtca atcacccgat cacgggtccg atgttctccg gagagcagca
     1441 gcagccgttc gtctgcacga cgattcagag tgcggtcggt cgccagccgc tggtcgattc
     1501 ggcgacaccg ccgggctaca aggtgaccga cgggacgggc gcgacgatcg gctacagccg
     1561 cgactgctcg atcgagacgt tcgtgacgta ctggtaccgc aggaacacgg gcggtggcct
     1621 ggtgccgctg ccggcgggcg cgcgaccggc cgacatgggc acggtccagc tcgcggacgg
     1681 acgcaccgtc gacttcgtcg tccggcgcga ggtgggctcg atcaaccgct tcctctacag
     1741 catcgcgatg ctcgcgccgg caccgggcgc cgacaacgcg cagcagaacg acacgagcct
     1801 gtggaacggc aagctgctct actggttcca gggcggcgtc gcgatcggcc acacgcaggg
     1861 caccgtgcat tcgggctcga tgaaccccga cgtgctgggc cagggcatgg ccatcgtcca
     1921 ttcgagcggc aacaacaccg gcacgcacta caacctcgag gtcgcgggcg agaccgcgat
     1981 gatgaccaag gagcgcttca tcg
//
LOCUS       DASPFR010000188         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1395496, whole genome shotgun sequence.
ACCESSION   DASPFR010000188 DASPFR010000000
VERSION     DASPFR010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395496"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2142)
                     /locus_tag="VD737_04795"
     CDS             complement(<1..2142)
                     /locus_tag="VD737_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HXW09910.1"
                     /translation="MEITRLPPADVLASLGSCEAGLDVPEVCKRQREFGPNRIEEIRA
                     APLSLRLAREFTHFFALILWVAAALAFIAESANPGGGMWQLGVAILGVILVNGVFSFM
                     QEYRAERAIAALRDLLPAETKVVRSGELTTVPAGTLVPGDLILVSEGDNVPADCRLIH
                     AQALRANFATLTGESRPLTRTAEATLSDNHLDARNLLLAGTSIVGGRGRAVVYATGMR
                     TEFGRIARLTQTVGDAGSPLQREIARLSRLVALLASVLGLAFFGLGVVVGLPFWQSAM
                     FGIGIIVANVPEGLLPTVTLSLAMATQRMARRHALVRHLPSVETLGSTTVICSDKTGT
                     LTQNTMSVREVYTPRLEASTSDLDRVADDEHLRRNLRLCHDLRRSRRDGLETWLGDPM
                     EIALAEFGAPASGPERVVEEIPFDSDRRRMSVVVQQDGRRWLYCKGAPEAVLKLCDAL
                     AVPGGSLPLDDARRAAVLEVQSAMAGRGLRVLGFAHRELRDAPSDESGLVFAGLVGLR
                     DPPRPEVMEAVRRCRSAGIRIVMVTGDHPRTAEATAREIGLVTTTRPLVIHGDRLKRM
                     SDAQLAIELDSDEVLFTRVTAEQKLRVVLALKAKGHVVAVTGDGVNDAPALKAADIGI
                     AMGLSGTDVAKESADMVLLDDNFASIVNAIEEGRAVYRNIRHFLTYILTSNVPELVPY
                     LAYVLFRIPLPLTVIQILAVDLGTDMLPALAL"
BASE COUNT          366 a          793 c          785 g          339 t
ORIGIN      
        1 cagcgcgagg gccggcagca tgtcggtgcc gaggtcgacc gcgaggatct ggatcacggt
       61 gagtggcagc gggatccgga acagcacgta tgcgaggtac ggcaccagtt ccgggacgtt
      121 cgaggtgagg atataggtca ggaagtggcg gatgttgcgg tagacggcac gcccttcctc
      181 gatcgcgttg acgatgctcg cgaaattgtc gtccagcagc accatgtccg cggattcctt
      241 ggcgacgtca gtgccgctca gccccatcgc gatgccgatg tcggcggcct tgagcgcagg
      301 cgcatcgttc acgccgtcgc ccgtgacggc gacgacgtgg cccttggcct tgagcgcgag
      361 caccacgcgc aacttctgtt ccgccgtgac tcgcgtgaac agcacctcgt ccgaatccag
      421 ctcgatcgcc agctgcgcgt ccgacatccg cttgagccgg tcaccgtgga tgacgagcgg
      481 cctggtcgtg gtgacgaggc cgatttcacg agccgtcgct tccgccgtcc gcggatggtc
      541 gccagtgacc atcacgatcc ggatcccggc gctgcgacag cgcctgaccg cctccatgac
      601 ctccggccga ggtgggtcgc gcaggccgac gagcccggcg aagacgagcc ccgattcgtc
      661 cgacggcgca tcgcgcagct cgcgatgggc gaacccgagc acgcgcagcc cgcgtcccgc
      721 catcgctgac tgcacctcga gcaccgcggc gcgtcgcgcg tcgtcgagcg gcagcgaccc
      781 gccgggcacg gcaagcgcgt cgcagagctt gagcacggct tcgggtgcgc ccttgcagta
      841 gagccagcgc cggccgtcct gctgcacgac gaccgacatg cgccgccggt cgctgtcgaa
      901 cggaatctcc tcgacgacgc gttccggccc ggacgcgggc gcgccgaact ccgcgagcgc
      961 gatctccatc ggatccccca gccaggtctc caggccatcc cgccggctgc ggcgcaggtc
     1021 gtggcacagg cggagattgc gacgcagatg ctcgtcgtcg gcgacgcggt cgaggtcgct
     1081 ggtggacgcc tcgagcctgg gcgtgtagac ctcacgcacg gacatcgtgt tctgcgtgag
     1141 cgtgccggtc ttgtcgctgc agatcacagt ggtcgagccg agcgtctcga ccgagggcag
     1201 gtgcctgacg agcgcatgac gcctcgccat gcgttgcgtc gccattgcca gcgacaacgt
     1261 gacggtcggc agcaggcctt ccggcacgtt cgccacgatg atgccgatgc cgaacatcgc
     1321 gctttgccag aacggcagcc cgacgaccac gccgagaccg aagaacgcca ggccgagcac
     1381 ggacgcgagc agcgcgacga ggcgcgacag gcgggcgatc tcccgttgaa ggggcgagcc
     1441 ggcgtcgccc acggtctggg tcaggcgggc aatgcgaccg aactccgtgc gcatgcctgt
     1501 cgcatagacg acggcgcgcc cgcgcccgcc gacgatcgag gtgcccgcga gcagcaggtt
     1561 gcgcgcatcg aggtggttgt ccgacagcgt cgcttcggcc gtgcgggtca gcggacgcga
     1621 ttcgccggtc agcgtcgcga aattggcgcg caacgcctgc gcatggatga gcctgcagtc
     1681 ggctggtacg ttgtcgcctt cggaaaccag gatcaggtcc cccggcacca gcgtgccggc
     1741 cggcacggtg gtcagttcgc cgctgcggac gaccttcgtc tctgccggca gcaggtcgcg
     1801 cagcgcagcg atggcgcgct cggcgcgata ctcctgcatg aacgagaaca cgccgttcac
     1861 gaggatcacg ccgaggatcg ccacgccgag ctgccacatc cccccgcctg ggttcgcgga
     1921 ctcggcgatg aaagccagcg ccgccgcaac ccacaggatc agcgcgaaga aatgcgtgaa
     1981 ttcccgcgcc aggcgcagcg agagaggtgc ggcgcgtatc tcctcgatcc ggttcgggcc
     2041 gaactcccgc tgccgtttgc agacttcggg aacgtccagc cccgcctcgc acgagcccag
     2101 gctcgcgagc acgtccgcgg gcggcagtcg cgtgatttcc atagcgcccc gaggacgggg
     2161 cacgggacat tttcccgctt accggatgaa cgcgcagcaa cgtgcggcgc ggcgggctct
     2221 cggactatgc ctaggcgggc gcggtcggta cgcccggccc cggctcactt gtcctcgttc
     2281 cag
//
LOCUS       DASPFR010000189         2945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_297934, whole genome shotgun sequence.
ACCESSION   DASPFR010000189 DASPFR010000000
VERSION     DASPFR010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297934"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /gene="folE2"
                     /locus_tag="VD737_04800"
     CDS             <1..901
                     /gene="folE2"
                     /locus_tag="VD737_04800"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF010200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HXW09911.1"
                     /translation="LRARRADSRILRRPFEASQPSMSRTAVPKLGTASVTPIEDVQGR
                     ADSRQIPINKVGIKDVFHPIRVRDRSGGDQHTVANFNMYVALPHNFKGTHMSRFVEIL
                     HLHEREISVESFRQMLTEMTERLDATSGHIEMTFPYFVMKQAPVTRVESVVKYDASLI
                     GEIHDGVEQMWIRVVVAATSLCPCSKKISDYGAHNQRSHITITARVREHVWIEELIDI
                     AEQEASCEVFGILKRPDEKYVTERAYDNPKFVEDIVRDVAVRLNAEERVLAYVVEAEN
                     FESIHNHSAYAQIERDKEEVASG"
     gene            complement(1059..2366)
                     /locus_tag="VD737_04805"
     CDS             complement(1059..2366)
                     /locus_tag="VD737_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXW09912.1"
                     /translation="MRTQILPLDPPQTTPDRIGKYYVVHEVGRGSTGAVYLSHDPFYG
                     RDVAIKLYHATTNDDAEGRNARRMFLGEAKMVGKLQHPNIVPIYDAGEEDGRRYVVTE
                     HIHGARTLNQYCRSNSLLPIDQVVSIMYKCAKALHYAHSRGVVHRDIKPSNILLTQEG
                     DVRIVDFGIALVADSDVSRLEGVAGSPAYMSPEQVQGFELDARSDLYSLGAVMYEMLC
                     GQRPFRAGALGKLLRQVVQQAPETLRSMRPEIPAELEPVVMKALEKEPDNRYRTGNEF
                     AGELTRVHQKLRASQAEIDDEERFAVLRKLRFFHDFSHAEIREVMRASTWCEYQPGEA
                     ILRAGDIDDRFYIVVSGNVRIGRGGDSLGTIPAGGCFGEASYGEGSRRDTLAEAQEAV
                     TLLKVGATLIEQASISCQLRFHKVFLRELIGRLQRGQKKVVSG"
     gene            complement(2396..2920)
                     /locus_tag="VD737_04810"
     CDS             complement(2396..2920)
                     /locus_tag="VD737_04810"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HXW09913.1"
                     /translation="MTTSPVDLPPSRAERARTRIPASALREPVHLLALGFGSGLAPVA
                     PGTFGSLVGLALALLALQAGWWTALGVAVLASVAGVWICGESARRLGVHDHPAIVWDE
                     VAGMMLVMLAAPATWWGAVLAFALFRVFDVWKPWPIREVDHGMRGGAGIMLDDILAAL
                     WAVAVLLLIRTYTT"
BASE COUNT          504 a         1010 c          964 g          467 t
ORIGIN      
        1 cctgcgtgcc cgccgcgcgg actcccgtat actcaggcgt ccgttcgagg ccagccaacc
       61 cagcatgagt cgcaccgccg ttcccaagct cggcaccgcc agcgtgaccc cgatcgagga
      121 cgtccagggt cgcgccgatt cccgccagat cccgatcaac aaggtcggga tcaaggacgt
      181 gtttcacccg atccgcgtgc gcgaccgctc gggtggcgac cagcacaccg tcgccaactt
      241 caacatgtac gtggccctgc cccacaactt caagggcacg cacatgtcgc ggttcgtcga
      301 gatcctgcac ctgcacgagc gcgagatctc ggtcgagtcg ttccggcaga tgctgaccga
      361 gatgaccgag cgcctcgacg ccacctccgg ccacatcgag atgacgttcc cctacttcgt
      421 catgaagcag gcgcccgtca ccagggtcga gagcgtggtc aagtacgacg cgagcctgat
      481 cggcgaaatc cacgacggcg tcgagcagat gtggatccgc gtcgtcgtcg cggcgaccag
      541 cctgtgcccc tgctcgaaga agatctccga ctatggcgcg cacaaccagc gttcgcacat
      601 cacgatcacg gcgcgcgtca gggagcacgt ctggatcgag gagctgatcg acatcgccga
      661 gcaggaagcc tcctgcgagg tcttcggcat cctgaagcgg cccgacgaga agtacgtgac
      721 cgagcgcgcg tacgacaacc ccaagttcgt cgaggacatc gtccgcgacg tggcggtgcg
      781 cctgaacgcc gaggagcgcg tgcttgccta cgtggtcgag gccgagaact tcgagtcgat
      841 ccacaatcac agcgcctatg cgcagatcga gcgggacaag gaagaagtgg ctagtggtta
      901 gtggctagtg gtcagtggct agtggttagt ggttagtggc tagtaggaag gctcgcatca
      961 cgcgtgcgac cgcaaccaga aaaaaggcgc cgagaggcgc cttcttcttt ccggcactag
     1021 ccactagcca ctagccacta gccactagcc actgaccact agccactaac cactttcttc
     1081 tgtccccgct gcagccgccc gatcagctcg cgcaggaaca ccttgtggaa gcggagctgg
     1141 cacgagatcg aggcctgctc gatcagcgtc gcgccgacct tgagcagcgt caccgcctcc
     1201 tgcgcctccg ccagggtgtc gcggcgcgaa ccctcgccgt agctcgcctc gccgaagcag
     1261 ccgcccgcgg ggatcgtccc gaggctgtcg ccaccccggc cgatgcggac gttgcccgac
     1321 accacgatgt agaagcggtc gtcgatgtcg ccggcgcgca ggattgcctc gcccggctgg
     1381 tactcgcacc acgtgctcgc ccgcatgacc tcgcggatct cggcgtgcga gaagtcgtgg
     1441 aagaagcgca gcttgcgcag gaccgcgaag cgctcctcgt cgtcgatctc cgcctggctc
     1501 gcgcgcagct tctggtgcac gcgcgtgagt tcgcccgcga actcgttgcc ggtgcggtag
     1561 cggttgtccg gctccttctc gagcgccttc atgacgacgg gctcgagttc ggccgggatc
     1621 tcggggcgca tcgagcgaag cgtctcgggc gcctgctgca cgacctgtcg cagcagcttg
     1681 ccgagcgcgc cggcgcggaa cgggcgctgg ccgcagagca tctcgtacat gacagcgccg
     1741 agcgaataga ggtcggagcg cgcgtcgagt tcgaagccct gcacctgctc gggcgacatg
     1801 taggcggggc tgcccgccac gccttcgagc cgcgagacgt cggaatccgc gaccagcgcg
     1861 atcccgaagt cgacgatgcg cacgtcgcct tcctgcgtga gcaggatgtt cgacggcttg
     1921 atgtcccggt gcacgacgcc gcgcgagtgc gcgtagtgca gcgccttcgc gcacttgtac
     1981 atgatcgaca cgacctggtc gatcggcagc aggctgttgc tgcggcagta ctggttgagc
     2041 gtgcgcgcgc cgtggatgtg ctcggtgacg acgtagcggc ggccgtcctc ctcgcccgcg
     2101 tcgtagatcg gcacgatgtt gggatgctgc agcttgccga ccatcttggc ttcgccgagg
     2161 aacatgcggc gcgcgttgcg gccttcggcg tcgtcgttgg tcgtcgcgtg atagagcttg
     2221 atcgcgacgt cgcgcccgta gaacgggtcg tgcgagaggt acacggcgcc ggtgctgcca
     2281 cgcccgactt cgtgcaccac gtagtacttg ccgatgcgat cgggggtcgt ctgcggcggg
     2341 tcgaggggca ggatctgcgt tcgcaccgcg gccggttcca tttttcttgc gtgactcacg
     2401 tcgtgtacgt tcggatcagc agcaggaccg cgaccgccca cagcgcggcc aggatgtcgt
     2461 cgagcataat gcccgctccg ccccgcattc cgtgatcgac ctcgcggatc ggccagggct
     2521 tccagacgtc gaacacgcgg aacagcgcga acgcgagcac ggccccccac cacgtcgcgg
     2581 gcgccgcgag catcaccagc atcatcccgg cgacttcgtc ccagacgatc gccggatgat
     2641 cgtgcacgcc gaggcggcgc gcgctctcgc cgcagatcca gacgcccgcg accgatgcca
     2701 gcacggccac gcccagcgct gtccaccagc ccgcctgcaa cgcgagcagc gcaagcgcga
     2761 ggccgaccag cgagccgaag gtaccgggtg cgacgggcgc gagaccggac ccgaatccca
     2821 aagccagcag gtgcaccggc tcgcgcagcg cggaggccgg aatgcgcgtg cgtgcgcgct
     2881 cggcgcgcga cggcggcagg tcaaccggcg aagtggtcat gaccgctcgc gctcaccggc
     2941 tcgtc
//
LOCUS       DASPFR010000190         5424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1275528, whole genome shotgun sequence.
ACCESSION   DASPFR010000190 DASPFR010000000
VERSION     DASPFR010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275528"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            298..1149
                     /locus_tag="VD737_04815"
     CDS             298..1149
                     /locus_tag="VD737_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXW09914.1"
                     /translation="MTAAPVEAADRYWPPIVDPATHQYTPGRWVWADLVTSDVAAAAD
                     FYGKVFGWTFETYGGEDDRETYTLVLAEGLPIGGMVFDARARKDATPSARWIGLLSVS
                     DPEAVAARVRSSGGEIVYAPAMLGERGETAVFKDPEGTLFGVVKSRNGDPPDYFGDVG
                     EWTWLDLWSNDVEKAAQFYRSVIGYETVPAGDTSSARSGMHLVSGGYVRAGIMQKRDP
                     KTTSVWLPYVRVADAKKSAQAAREAGGRVIYEPAPLGTAMVGIVADPTGAPVGIVEMA
                     ASPETRQ"
     gene            1146..1568
                     /locus_tag="VD737_04820"
     CDS             1146..1568
                     /locus_tag="VD737_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09915.1"
                     /translation="MSARQQLDRWIGRSRVVACTVALVLAAGCASDGGGGGGGVSTSM
                     HVGVGYGSYYGPGWYGGYYEPYPPVVVVDPDRPVGDRPDRPTTLPADVAGARPQPAGM
                     ASSNRATTAPSRPSNVSRPTSRPSPRPATRPMPRMGRR"
     gene            complement(1611..2180)
                     /locus_tag="VD737_04825"
     CDS             complement(1611..2180)
                     /locus_tag="VD737_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HXW09916.1"
                     /translation="MKMLGRLAATVGLAFATSASAQQAATAPPAAAPSSLSSSLGVVV
                     FPAKGQAAEVQSRDEGECYAWSKGQTGIDPNAPAPAPAPASTASATEANRGGGERVRG
                     AARGAAAGAIIGEVANDDASDGAAIGATVGVLKGGAERRRNKEQAEQQQATAQKEAAA
                     QQQAANQQQRDQFNKGFAACLEAKGYTVK"
     gene            complement(2290..4626)
                     /locus_tag="VD737_04830"
     CDS             complement(2290..4626)
                     /locus_tag="VD737_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXW09917.1"
                     /translation="MEITKLAAAVAAILTTGQALQAQAAELPGAAGAAGDVEEIIVTA
                     QRREESIQDVPLSVQAFTGETLAEAGVTSVLDLPRLAPNFNAMRGTQTANVRLSIRGV
                     GASSNSAIDPSIGTFIDGVYVPRPGSLFGSMNDIASVEVLRGPQGTLFGRNSTVGAVL
                     LRSVDPQDEFGGSAELTYGNYDEQKFAGTLNVPVGERFAVRVAGLTDSRDGYAHNRFD
                     GRDFATLESDAFRIGATWDITDDVTWTVKYDRSRMQGDGKAELEIDPDTLTDASRARL
                     TLITGGNPPDLDDAFDRKSNQRITGDVDDDQWGLVSNLTWDLANGHTVRLLGGYRSWN
                     NEQLESDVLFMPADFLARTGRFESTSQSYELQLISPQDELLNGRLDYVAGLYYFQEDY
                     FVGEQFGLGAQFCGLVPAAQRPACAASANKANATMLDFDQDAENLAAYAQTDIGLTDA
                     VDLVLGARWTKDDKTGTFVQTVNNPFGALLRAAENTALDLGEDAFTYRVGLNWAPNQD
                     LLVFGSYSTGYKSGGFNSGGGNAALGQRRLFDKETTDNYEVGAKWTLADGRAHLNGTA
                     FLMQLDDFQDRAFDGQSFNVINAGSVRNQGIEIDGDWSPQDWLSLYASVGYLDAEFDE
                     YDNASCLPYPAQVNPTCTQDLAGERPVFAPELQASTGVQVQGDFGGSGVGYMLRTDVA
                     YMDDINVNQINDNNPQGIQPAFTLVSARFSLYFGAAQRYALTFWGDNLTDEDYCTGKV
                     AQPFDNLLGLRDPVSGGTVMRCQVNVPRTYGVSLKASF"
     gene            complement(4715..>5424)
                     /locus_tag="VD737_04835"
     CDS             complement(4715..>5424)
                     /locus_tag="VD737_04835"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HXW09918.1"
                     /translation="DITRAEHVAFARRFGDLEDHPVAGSDPDHPGLVRIYKDLDTPPE
                     HYENAWHCDGTWRETPAMGCVLRCVESPEVGGDTIWADMVAAYEGLPGHVKSQIEGLR
                     ARHSIEATFGARLPIEQRQAMKAKFPDAEHPVVRTHPETREKALFVNAFTTHIVNFHT
                     PANVRHGQDYAPGASNLLSYLIGRVNIPDYQVRWRWRKDSVAIWDNRCTQHYAVQDYW
                     PAVRRMERAGIVGDRPF"
BASE COUNT          809 a         1837 c         1828 g          950 t
ORIGIN      
        1 ccgcatcctt gaacagcttg atggtgtcgt cgtacttgtc cgcaaacgcc ggccccgtgg
       61 cgagcaggag cgccgcgaac gtcggtaaca gtgaactgag acggaacatg gccatctcct
      121 tcatggtggc tgcctcatgc attctagtcc cgtggcggcg aggcggcttt cgttggttgt
      181 acggacgacg actgggttac catcggccga cgtcgagtaa cggagaagga agtggcgaca
      241 cccttggttt gcgggagcag gcgcgtcaag tcgctgctta tgtgttgtct agtgtcgttg
      301 accgccgcac ccgtcgaggc ggcggatcgt tactggccgc ccatcgtcga ccccgccacg
      361 caccagtaca cccccggtcg ctgggtctgg gccgacctgg tgacctcgga cgtcgccgca
      421 gcggccgact tctatggcaa ggtcttcggc tggacgttcg agacctacgg aggcgaggac
      481 gaccgcgaga cctacacgct cgtgctggcc gagggcctgc cgattggcgg catggtgttc
      541 gatgcgcggg cccggaaaga cgcgactcct tcggcacgct ggatcggcct gctctcggtc
      601 agtgaccctg aggccgttgc ggcacgcgtg aggagcagcg gcggtgagat cgtctacgcc
      661 cccgccatgc tcggcgagcg tggcgagacc gccgtgttca aggaccctga gggtacgctg
      721 ttcggcgtcg tgaagtcgcg caacggcgat ccgcccgact atttcgggga cgtcggcgaa
      781 tggacgtggc tcgacctctg gtcgaacgac gtcgagaagg ccgcgcagtt ctaccgcagc
      841 gtgatcggct acgagacggt gcccgcgggt gatacgtcgt cggcacgcag cggcatgcac
      901 ctcgtctccg gcggttacgt ccgtgccggg atcatgcaga agcgcgatcc gaagaccacg
      961 tcggtgtggc tgccctacgt gcgcgttgcc gacgcgaaga agtccgccca agccgcgcgc
     1021 gaagcgggcg gccgcgtgat ctacgagccc gcgccgctcg gaacggcaat ggtcgggatc
     1081 gtcgccgacc ccaccggagc gccggtggga atcgtggaaa tggccgcaag cccggagaca
     1141 cggcagtgag cgcacgacag caactcgatc ggtggatcgg ccgcagccga gtcgtcgcgt
     1201 gcaccgtcgc actggtgctc gccgccgggt gcgcgagcga cggtggcgga ggcggtggcg
     1261 gcgtgtcgac cagcatgcac gtcggcgtgg gctacggcag ctactacggt ccaggctggt
     1321 acggcggcta ctacgagccc tatccacccg tggtcgtcgt cgatcccgac cgacccgtcg
     1381 gtgatcgtcc cgaccggcct accacgctgc ccgcggacgt cgccggcgcc aggccgcagc
     1441 cggcggggat ggcgtcgtcg aatcgtgcga cgacggctcc gagtcgcccg tcgaacgtca
     1501 gccgcccgac cagccgaccg tcgccgcgtc cggcgacacg tccgatgcca cggatggggc
     1561 ggcgctaaac gccggattga agccagtgga cgcaggctcc gggcgccagg tcacttgacc
     1621 gtgtagccct tggcctcgag gcacgccgcg aagcccttgt tgaactgatc gcgctgctgc
     1681 tggttcgccg cctgctgttg agccgccgcc tccttctgcg cggtcgcctg ttgctgctcg
     1741 gcctgttcct tgttgcggcg acgttccgcg ccacctttca gcacgccgac cgtcgcaccg
     1801 atggcggcgc cgtcgctggc atcgtcgttg gccacttcac cgatgatcgc gccggccgcg
     1861 gcgccacgcg ccgccccgcg cacgcgctcg ccaccgccac ggtttgcctc cgtcgcggac
     1921 gccgtcgaag cgggggccgg cgcgggcgcg ggagcgttgg gatcgatgcc ggtctggcct
     1981 ttcgaccacg catagcactc gccctcgtcg cggctctgta cttcggcggc ctgtcccttg
     2041 gcggggaaga ccaccacgcc cagggaactc gacagtgatg agggggccgc cgcaggcggg
     2101 gccgttgccg cctgctgtgc gctcgcgctc gtcgcaaagg cgaggccgac cgtggctgcg
     2161 agcctgccga gcatcttcat gagcgttccc cgctggtctc ctgtcgacat catggagccg
     2221 ccacgggtcc tgctcaagcg aatccgcacc cgggagatcc cgccgcagga cctcccgggt
     2281 ggtcgtggct cagaacgacg ccttcagcga caccccgtac gtgcggggca cgttcacctg
     2341 gcagcgcatg acggtgcccc cactcacggg atcgcgcagg cccagcaggt tgtcgaacgg
     2401 ctgggcaacc ttgccggtgc agtagtcttc gtcggtcagg ttgtcgcccc agaaagtcag
     2461 ggcgtagcgc tgcgccgcac cgaagtacag cgagaaccgc gccgacacca gcgtgaatgc
     2521 aggctggatg ccctgcgggt tgttgtcgtt gatctggttg acgttgatgt cgtccatgta
     2581 ggccacgtcg gtgcgcagca tgtaacccac gccgctgcca ccgaaatcgc cctggacctg
     2641 cacgcccgtc gacgcctgca gctcgggtgc gaacaccggg cgctcacccg cgaggtcctg
     2701 cgtgcaggtc gggttgacct gcgccgggta cggcaggcac gacgcattgt cgtactcgtc
     2761 gaactccgcg tcgagatagc cgaccgacgc gtacagcgag agccagtcct gcggcgacca
     2821 gtcgccgtcg atctcgatgc cttgattgcg gacgctgccc gcgttgatga cgttgaagct
     2881 ctggccgtcg aacgcacggt cctggaaatc gtcgagctgc atcaggaacg ccgtgccatt
     2941 caggtgcgcg cgaccgtctg ccagcgtcca cttggcgccc acttcataat tgtccgtcgt
     3001 ttccttgtcg aacagccggc gctggccgag cgccgcgttg ccgccgccgg agttgaagcc
     3061 tccggacttg taacccgtcg agtagctgcc gaacacgagc aagtcctggt tcggcgccca
     3121 gttcaggccg acgcggtacg tgaacgcgtc ctcgccgagg tcgagcgcag tgttctccgc
     3181 cgcgcgcagc aaggcaccga acgggttgtt gaccgtctgc acgaacgtgc cggtcttgtc
     3241 gtctttggtc cagcgcgcgc cgagcaccag gtcgaccgca tcggtgaggc cgatgtccgt
     3301 ctgcgcgtat gccgccaggt tctcggcgtc ctggtcgaag tcgagcatgg tggcgttcgc
     3361 cttgttggcg ctcgcggcgc acgcgggacg ctgcgccgcg ggcacgaggc cgcagaactg
     3421 cgcgcccagg ccgaactgct cgccgacgaa gtagtcttcc tggaagtaat agagtccggc
     3481 cacgtagtcg agccggccgt tcaacagctc gtcctgcggc gagatcagct gcagttcgta
     3541 cgactggctg gtgctctcga accgccccgt gcgggcgagg aaatcggcgg gcatgaacag
     3601 cacgtccgat tcgagctgtt cgttgttcca gctgcgatag ccgccgagca ggcggacggt
     3661 gtggccgttc gcgagatccc aggtcaggtt gctgacgaga ccccattggt cgtcgtccac
     3721 gtcgcccgtg atgcgctggt tgctcttccg gtcgaaggcg tcgtcgaggt cgggcggatt
     3781 gccgccggtg atcagcgtga gccgcgcgcg cgacgcgtcc gtcagagtgt ccggatcgat
     3841 ctcgagctcc gccttgccgt cgccctgcat tcgcgaccgg tcgtacttga ccgtccaggt
     3901 cacgtcatcc gtgatgtccc acgtcgcgcc gatcctgaac gcgtcgctct cgagcgttgc
     3961 gaaatccctg ccgtcgaagc ggttgtgggc atagccgtcg cgcgagtccg tgagacccgc
     4021 gacgcgcacc gcgaagcgct cgcccaccgg gacgttgagc gtaccggcga atttctgttc
     4081 gtcgtagttg ccataggtca gctcggcgct gccgccgaac tcgtcctgcg gatccaccga
     4141 gcggagcagc accgccccca cggtcgagtt gcgcccgaac agcgtgccct gcggcccccg
     4201 cagaacttcg acgctcgcga tgtcgttcat cgagccgaac agcgagccgg gtcgcggcac
     4261 gtacacgccg tcgatgaacg tgccgatgct cgggtcgatc gcgctgttgc tggaggcgcc
     4321 cacgccgcgg atgctcagcc gcacgttcgc ggtctgcgtg ccgcgcatcg cgttgaagtt
     4381 cggcgcgagc cgcggcaggt cgagcacgct ggtcacgccg gcctcggcga gcgtctcgcc
     4441 cgtgaaggcc tgcaccgaaa gcggcacgtc ctggatgctc tcctcgcgcc gctgtgccgt
     4501 gacgataatc tcctcgacgt cccctgccgc tcccgcggca ccgggcaact cggcggcctg
     4561 ggcctgcagg gcctgtccgg tcgtcaggat tgccgccacc gcggcggcca gcttcgtgat
     4621 ctccatccgt cgcatttcct tacccccacg catgtgcgtt gactcccgcg acgccgaggc
     4681 gccgcgtcaa ttccggtcga aagaaaaaaa gaattcagaa gggccggtcg ccgacgatgc
     4741 cggcacgttc catccggcga accgccggcc agtagtcctg gaccgcgtag tgctgcgtac
     4801 agcggttgtc ccagatcgcg acgctgtctt tgcgccagcg ccaccgcacc tgatagtccg
     4861 ggatgttcac gcgaccgatc aggtagctca gcaggttgct tgcgccaggc gcatagtcct
     4921 gcccgtggcg cacgttggcg ggcgtgtgga agttgacgat gtgtgtcgtg aacgcattca
     4981 cgaacagcgc cttttcgcga gtttccggat gcgtgcgcac gaccgggtgt tcggcatcgg
     5041 ggaacttcgc tttcatcgcc tgacgttgtt cgatgggcag ccgcgcgccg aaggtcgcct
     5101 cgatgctgtg gcgtgcgcgc aggccctcga tctgcgactt gacgtgtcca ggcaggccct
     5161 cgtacgccgc caccatgtcg gcccagatcg tgtcgccgcc gacttccggc gactcgacgc
     5221 agcgcaggac gcagcccatg gccggcgttt cgcgccaggt accgtcgcag tgccacgcgt
     5281 tctcgtaatg ctccggcggc gtgtcgaggt ccttgtagat gcggaccagc cccgggtgat
     5341 ccgggtcgct gccggccacc ggatgatctt cgagatcgcc aaaccgacgt gcgaaggcga
     5401 cgtgctcggc gcgcgtgatg tcct
//
LOCUS       DASPFR010000191         4859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1396342, whole genome shotgun sequence.
ACCESSION   DASPFR010000191 DASPFR010000000
VERSION     DASPFR010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1396342"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /gene="cysC"
                     /locus_tag="VD737_04840"
     CDS             <1..879
                     /gene="cysC"
                     /locus_tag="VD737_04840"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HXW09919.1"
                     /translation="PMLPGRPYWIKIGTRQATATITEPKYKVNVNTLEHLAAKKLELN
                     EIGVCNLSLDQSIAFDSYQDCRETGSFILIDRLSNATVGAGMIHFALRRSQNIHWQAL
                     DVNRKSRAAIKGQRPAVLWLTGLSGAGKSTIANLVEKKLHALGKHTYLLDGDNVRHGL
                     NRDLGFTDADRVENIRRVAEVSKLMVDAGLIVLVSFISPFRAERRMARALFGPGEFHE
                     VHVDTPLSIAETRDPKGLYKKARRGELKNFTGIDSPYEAPESPEIYVDTTRNASEQSA
                     EAIVEYLERGGWLSAS"
     gene            complement(910..2322)
                     /locus_tag="VD737_04845"
     CDS             complement(910..2322)
                     /locus_tag="VD737_04845"
                     /inference="COORDINATES: protein motif:HMM:NF021382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HXW09920.1"
                     /translation="MRERLYVGPKIRALREARGLRLEACARQLELSTSYLSQIETNQR
                     PVTPRVLIALSRYFGISPGEFATDDEGRLIADLREATADLAIDAASPSLAELKLAAAT
                     TPTLARQFLALHRAYRALDERMLRFDETVKIRTEDAPDSRIPYQEVRDFFHYRDNYVD
                     ELDVAAESLAAEIGVGGTGGIVAALERALLDRHGIRVARAPSTEGYIRRFDADTSTLT
                     LGRDLAEPTRAFQLATQLADLTFGPLFEQVLDTGGLKSVTAREIARIGLGNYAAGALT
                     MPYRHFAASAMALRHDIEQLQHRYTASFEQVSHRLSTLQRPGARGVPFYFVRVDQAGN
                     ITKRHSSTRFQFARFGGACPLWNVHEAFGHPGRIMVQLAEMPDGVRYLCIGRSIVKRS
                     GNYLQPNRHYAIGIGCEVAHAEKVIYSEGLRLDGAAVPVGVSCRICERPNCHQRAFPP
                     LGRKLTVQPGVRRVVPYAIE"
     gene            2451..3362
                     /gene="prpB"
                     /locus_tag="VD737_04850"
     CDS             2451..3362
                     /gene="prpB"
                     /locus_tag="VD737_04850"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004973459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HXW09921.1"
                     /translation="MPIKLANGFSSAGARLRAALEVENPLQVVGAINAYAALLAERAG
                     FRALYLSGAGVANASFGLPDLGITSLNDVCEDVRRITSATDLPLLVDADTGWGAAFNI
                     SRTISDLIRSGAAGCHLEDQVQAKRCGHRPGKALVTADEMADRIKAAVDGRTDDQFVI
                     MARTDAHAVEGQQAAIDRAQHYVAAGADMIFAEALTTLDEYRQFTAAVKVPVLANITE
                     FGKTPLYTVQELASAGAQLVLYPLSAFRAMSKAAEAVYGALRQEGTQKSVTASMQTRA
                     ELYEVLGYHDYERKLDELFAREAPVKP"
     gene            3407..4582
                     /gene="prpC"
                     /locus_tag="VD737_04855"
     CDS             3407..4582
                     /gene="prpC"
                     /locus_tag="VD737_04855"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HXW09922.1"
                     /translation="MSSSTTTSPSETPAVGFKPKKSVALSGVPAGNTALCTVGRSGND
                     LHYRGYDILDLADRCEFEEVAHLLVHGKLPNAAELRSYKDKLRRLRGLPVPVRQALEA
                     LPAASHPMDVLRTGASVLGCAMPEKDDHNTAGARDIADRLVASLGSMLCYWYHYSHSG
                     LRITVETDDDSVGGHFLHLLHRRQPPASWVKAMHVSLNLYAEHEFNASTFTGRVIAGT
                     GSDIYSCITGAIGALRGPKHGGANEVAFEIQQRYENPDDAEADIRARVERKEVIIGFG
                     HPVYTISDPRNKVIKEVARKLSTEAGDMKMFDIADRLETVMWDVKKMFPNLDWFSAVS
                     YHVMGVPTAMFTPLFVIARTTGWSAHVIEQRIDGKIIRPSANYTGPENLAFVPLDRR"
     gene            4597..>4859
                     /locus_tag="VD737_04860"
     CDS             4597..>4859
                     /locus_tag="VD737_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09923.1"
                     /translation="MKRLIGLCAWLACLPAFGADAVSELQLRRAGNESGVLRAVIPEG
                     TVGFCRDQPSVAKAVEAHVHGSRELRLLECFVQPAARVALQPV"
BASE COUNT          832 a         1639 c         1645 g          743 t
ORIGIN      
        1 ccgatgctcc ccgggcggcc ctactggatc aagatcggca ccaggcaggc caccgcgacc
       61 atcaccgagc ccaagtacaa ggtcaacgtc aacacgctcg agcacctcgc ggccaagaag
      121 ctcgagctca acgagatcgg cgtctgcaac ctctcgctcg accagtcgat cgcgttcgat
      181 tcgtaccagg actgccgcga gacgggctcg ttcatcctga tcgaccggct gtcgaacgcg
      241 acggtgggcg cgggcatgat ccacttcgcg ctgcgccgct cgcagaacat ccattggcag
      301 gcgctcgacg tcaaccgcaa gtcccgggcc gcgatcaagg gccagcgccc cgcggtgctg
      361 tggctcacgg gcctctccgg cgccggcaag tccacgatcg cgaacctggt cgagaagaag
      421 ctgcacgcgc tcggcaagca cacctacctg ctcgacggcg acaacgtgcg ccacggcctc
      481 aaccgcgacc tcggcttcac cgacgccgac cgtgtcgaga acatccgccg cgtcgcggag
      541 gtgtcgaagc tgatggtcga cgccgggctg atcgtgctcg tgtcgttcat ctcgccgttc
      601 cgggcggagc gacggatggc gcgcgcgttg ttcgggcccg gcgagttcca cgaggtgcac
      661 gtcgacacgc cgctgtcgat cgcggagaca cgcgacccga agggcctgta caagaaggcg
      721 cgccgcggcg agctcaagaa cttcaccggc atcgattcgc cctacgaggc gccggagagc
      781 cccgagatct acgtcgacac cacgcgcaac gcatcggaac aatccgccga ggcgatcgtc
      841 gagtacctcg agcgcggcgg ctggctgagc gcctcctgac ctctcccggc gcaccgggag
      901 aggtgaggac tattcgatcg cgtatggcac cacgcggcgc acgccgggct gcacggtcag
      961 cttgcggccg agcggtggga acgcgcgctg gtgacagttc ggccgttcgc agatgcgaca
     1021 actgacgccc accgggacgg cggcaccgtc cagtcgcagc ccttcggagt agatcacctt
     1081 ttcggcgtgc gcgacctcgc agccgatgcc gatcgcatag tgtcggttgg gctgcaggta
     1141 gttgcctgac cgcttgacga tgctgcggcc gatgcagagg tagcgcacgc cgtcgggcat
     1201 ctccgcgagc tgcaccatga tgcggcccgg gtggccgaac gcctcgtgca cgttccagag
     1261 cggacaggcc ccgccgaagc gcgcgaactg gaaccgcgtc gagctgtggc gcttggtgat
     1321 gttgcccgcc tggtcgacgc gcacgaagta gaacggcacg ccgcgggcgc cgggccgctg
     1381 cagcgtgctc aggcggtgcg agacctgctc gaagctcgcc gtgtagcggt gctgcagctg
     1441 ctcgatgtcg tgacgcaggg ccatcgcgga cgccgcgaag tgccggtacg gcatcgtcaa
     1501 cgcaccagcg gcgtaattgc cgaggccgat gcgggcgatc tcgcgcgcgg tcaccgactt
     1561 gaggcccccc gtgtcgagca cctgctcgaa cagcgggccg aacgtgaggt cggcgagctg
     1621 ggtcgccagc tggaaggcgc gggtcggctc agcgaggtcg cgaccgagcg tcagcgtgct
     1681 cgtatcggcg tcgaaccgcc ggatgtaccc ctcggtcgat ggggctcgtg ccacccgtat
     1741 cccgtggcgg tcgagcagcg ctcgttcgag tgcggcgacg atcccgcccg tgccccccac
     1801 gccgatctcg gccgcgagcg attcggccgc gacgtcgagc tcgtcgacgt agttgtcgcg
     1861 gtagtggaag aagtcgcgga cctcctggta cggaatccgc gagtccggcg cgtcctcggt
     1921 cctgatcttc accgtctcgt cgaagcgcaa catgcgctcg tcaagggcgc gatacgcccg
     1981 gtgcagcgcg aggaactgtc gcgcgagcgt cggcgtggtg gcagcggcga gcttgagttc
     2041 cgcgaggctg ggcgaggcgg cgtcgatggc caggtcggcc gtcgcctcgc gcaggtcggc
     2101 gatcaggcgg ccttcgtcgt cggtcgcgaa ttcccccggg ctgatgccga agtatcgcga
     2161 cagcgcgatc agcaccctgg gagtgacggg gcgctggttg gtctcgatct gcgagaggta
     2221 gctggtcgaa agctcgagct ggcgcgcgca ggcctcgagc ctgagcccgc gggcttcgcg
     2281 cagcgcacgg atcttcgggc cgacgtagag tctttcgcgc atcggttttg cagttttgca
     2341 ggagcggcca agcgcatcga gcatatacgc caaatttcac aaccctgcca ccgcgcataa
     2401 aggcgcaaaa tatcgagcaa ttttgtggtt cgtcatccgg tgatgtcggc atgcccatca
     2461 aattagcaaa tggattctcg tcggcgggcg cacggctgcg agcggcgctc gaggtcgaga
     2521 acccgctgca ggtcgtcggc gcgatcaacg cctatgcggc gctgctcgcc gagcgggccg
     2581 gcttccgcgc gctctacctg tcgggcgccg gcgtggccaa cgcctcgttc ggcttgccgg
     2641 acctcggcat cacaagcctc aacgacgtct gcgaggacgt ccgccggatc acctcggcca
     2701 cggacctgcc cctgctcgtg gatgcagaca ccggctgggg tgccgccttc aacatctcgc
     2761 ggacgatctc ggacctgatc aggtcggggg ccgccggctg tcacctcgag gatcaggtcc
     2821 aggccaagcg ctgcggacac cgacccggca aggccctggt cacggcggac gagatggcgg
     2881 accggatcaa ggcggccgtc gacggccgca cggacgacca gttcgtcatc atggcgcgta
     2941 ccgacgccca cgcggtggag gggcagcagg ccgcgatcga ccgggcccag cactacgtcg
     3001 ctgccggcgc ggacatgatc ttcgcggagg cgctcacgac gctggacgaa tatcggcagt
     3061 tcacggcggc cgtgaaagtg cctgtgctgg ccaacatcac cgaattcggc aagacgccgc
     3121 tctacaccgt gcaggagctc gcgagcgccg gcgcgcagct cgttctgtac ccgctgtccg
     3181 cgttccgtgc catgtcgaag gcggccgaag ccgtctacgg cgccctgcgc caggaaggca
     3241 cgcagaagag cgtgaccgcc agcatgcaga cgcgcgccga gctctacgaa gtgctcggct
     3301 accacgacta cgaacgcaag ctcgacgaac tcttcgcgcg agaggctccg gtgaagccct
     3361 gacccgccgc gcggaacgca cgtacacacc aggacgagag cgcatcatga gcagcagcac
     3421 caccacgagc cccagcgaaa ctcccgcggt cggcttcaag ccgaagaagt ccgtcgcgct
     3481 gtccggcgtg ccagcgggca acaccgcttt gtgcaccgtc ggccgcagcg gcaacgacct
     3541 gcactaccgc ggctacgaca tcctggacct cgccgacagg tgcgaattcg aggaagtcgc
     3601 gcacctgctg gtgcacggca agctgccgaa cgcggccgag ctcaggagtt acaaggacaa
     3661 gctccgtcgg ctgcgcggcc tgccggtgcc ggtgcgacag gcactcgagg ccttgccggc
     3721 cgcgagccac ccgatggacg tgctgcgcac gggcgcgtcg gtgctcggct gcgcgatgcc
     3781 cgaaaaggac gaccacaaca cggccggtgc gcgcgacatc gccgaccggc tcgtcgcgag
     3841 cctcggctcg atgctctgct actggtatca ctacagccac tccgggctgc gtatcaccgt
     3901 cgagaccgac gacgattcgg tcggtggtca cttcctgcac ctgttgcatc gccgccagcc
     3961 gcccgcctcg tgggtcaagg cgatgcacgt ctcgctcaac ctgtacgccg agcacgagtt
     4021 caacgcctct acgttcacgg gccgagtgat cgccggcacg gggtccgaca tctattcctg
     4081 catcacgggc gcgatcggcg cgttgcgcgg accgaagcac ggcggcgcga acgaagtcgc
     4141 gttcgagatc cagcagcgtt acgaaaatcc ggacgacgcc gaagccgaca tccgcgcgcg
     4201 cgtcgagcgc aaggaagtca tcatcggctt cggccatccg gtctacacga tcagcgaccc
     4261 gcgcaacaag gtgatcaagg aagtcgcgcg caaactgtcg acggaagccg gcgacatgaa
     4321 gatgttcgac atcgccgatc ggctcgagac cgtcatgtgg gacgtcaaga agatgtttcc
     4381 caacctcgac tggttcagcg ccgtgtcgta ccacgtcatg ggcgtgccca ccgcgatgtt
     4441 cacgccgctg ttcgtgatcg cgcgcacgac gggctggagc gcccacgtga tcgagcagcg
     4501 gatcgacggc aagatcatcc ggccgagcgc gaactacacg gggccggaaa acctggcttt
     4561 cgtcccgctc gacaggcgct gaccggggga agtacggtga aacgactgat tgggttgtgt
     4621 gcgtggttgg catgcctgcc ggcgttcggc gcggacgcgg tgagcgagtt gcagctgcgg
     4681 cgcgccggca atgagtccgg cgtgctgcgc gccgtgatcc ccgaggggac cgtcggcttt
     4741 tgccgcgacc agccctcggt ggcgaaggcg gtcgaagccc acgtgcacgg cagccgggag
     4801 ttgcgcttgc tcgagtgctt cgtgcagccg gcggcgcgcg tcgcgctgca gccggtcta
//
LOCUS       DASPFR010000192         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1276075, whole genome shotgun sequence.
ACCESSION   DASPFR010000192 DASPFR010000000
VERSION     DASPFR010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1276075"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VD737_04865"
     CDS             complement(<1..400)
                     /locus_tag="VD737_04865"
                     /inference="COORDINATES: protein motif:HMM:NF020031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXW09924.1"
                     /translation="MNLHEYQSKQLFARYGIPVPQGFVASTPEEAVDAAGRLGGPVWV
                     VKAQVHAGGRGKAGGVKVVKSKDDVRKAAAGMLGQKLKTKQTVGDGLPINRVYVESGS
                     KIDRELYLSLLLNRDTSRIAFVASAAGGMDI"
     gene            570..2216
                     /locus_tag="VD737_04870"
     CDS             570..2216
                     /locus_tag="VD737_04870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXW09925.1"
                     /translation="MAQAVQPGLVSTGRNPVDADLPWRVLSLLNVYRLLLPMLLLLVF
                     FFDAPTRSVGSQQPGLLLATAVAYFTFALLCIRSIARRAPSPTWQAMVQLAVDAVAMA
                     MFIHSSGGVASGLGTLLVLPAGATATIVERRYALLGTAIITIALLLETTISFLQGEGQ
                     TSDFLITGLTGASLFAITLLAIPYARRLRESEALVKQRDIDIANLNELNQFIVQNLRE
                     SILVVDEFDRVRLINETAARLLRGAPVLTGTALSLVSPRLAYLLDAWRQQTSDRRDVN
                     GEVVGADGGTAIRPHFVALSQVGSGPVLIFLEDTSVVAEQAQQSKLAALGRLSASIAH
                     EIRNPVGAMSHAGQLLRESPSLTPEDRDLTEIIEKNGVRVSRIIENVLQLSRRDTTRQ
                     ERFDVVDWIGTFVREYVETLQIDANRFHIEAPSHGRSIEVEFDPSHLHQVVWNLCDNA
                     LRHGGAEGASHVELHAGRIASTGRPFLQVCDRGEGIDPAQAERIFEPFFTTGHGGTGL
                     GLFISRELCQTNGALLVYEPRPGGGSIFRVIFADPRRWKG"
     gene            2213..>2625
                     /locus_tag="VD737_04875"
     CDS             2213..>2625
                     /locus_tag="VD737_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXW09926.1"
                     /translation="MNDRARLSRPRALVVDDEPDIRDLLSMTLRRMQVDVETASDRAS
                     AIRRLGSERFDLVLTDMRLPDGDGLDIVQWIQQQRPGVPVAVITAHGNVETAVRALKL
                     GAFDFISKPLDLVALRKMIQATLKLREDDTAPHDET"
BASE COUNT          399 a          940 c          863 g          423 t
ORIGIN      
        1 cgatgtccat gccgcccgcc gccgaggcga cgaacgcgat ccggctcgtg tcgcggttga
       61 gcagcaggct caagtacagc tcgcggtcga tcttcgaacc cgactccacg tagacccggt
      121 tgatcggcag cccgtcgccg accgtctgct tggtcttgag cttctgcccg agcatgccgg
      181 cggcggcctt cctgacgtcg tccttcgact tgacgacctt gaccccacct gccttgccgc
      241 ggccgcccgc gtggacctgc gccttgacga cccagaccgg cccgccgagc cgcccggccg
      301 cgtcgacggc ctcctcgggc gtggacgcga cgaagccctg cggcacaggg atgccgtagc
      361 gggcgaacag ttgcttcgac tggtactcgt ggaggttcat ggggctcgtg ggggcgggac
      421 aaacgagccc gaaagtgtag caaatcgacc ccctctcccg ccccgcgaga gagggttggg
      481 gtgtgggtct ctttacccct gttctccgag caccggggtg aggccctctt cgcactttcc
      541 ccgttgtagg actgctacag tccgcgccca tggcgcaagc agtgcagccg ggcctggtat
      601 cgaccgggcg gaatcccgtg gacgcggacc tgccgtggcg cgtgttgtcg ctgctcaacg
      661 tctatcggct gctgctgccg atgctgctgc tgctggtgtt cttcttcgac gcgccgaccc
      721 gcagcgtcgg cagccagcaa ccgggcctgt tgctcgcgac cgccgtcgcc tatttcacgt
      781 tcgcgctgct ctgcatccgg tcgatcgcgc gacgcgcccc ctcgcccacg tggcaggcga
      841 tggtccagct cgcggtcgac gcggtcgcga tggcgatgtt catccactcg agcggtggcg
      901 tcgcgagcgg cctcggcacg ctgctggtgc ttccggccgg cgccacggcg acgatcgtcg
      961 agcgtcgcta cgcgctgctc ggcaccgcca tcatcacgat cgcgctgctg ctcgagacga
     1021 ccatctcgtt cctgcagggc gaaggccaga cctccgattt cctgatcacc gggctcacgg
     1081 gcgccagcct gtttgcgatc acgctgctcg cgattcccta cgcccggcgc ttgcgggaga
     1141 gcgaggcgct ggtcaagcag cgcgacatcg acatcgccaa cctcaacgag ctgaaccagt
     1201 tcatcgtgca gaacctgcgc gagagcatcc tcgtggtcga cgagttcgac cgcgtccgcc
     1261 tgatcaacga gaccgcggcg cgcctgctcc gcggagcgcc cgtgctgacg gggaccgcgc
     1321 tcagcctcgt gtcgccgagg ctcgcgtacc tgctggacgc gtggcgccag cagacctccg
     1381 accgccgcga cgtcaacggc gaggtcgtcg gggccgacgg cggcacggcg atccgcccgc
     1441 acttcgtcgc gctgtcgcag gtcggctcgg gccccgtgct gatcttcctg gaggacacga
     1501 gcgtcgtcgc cgagcaggcc cagcagtcga aactcgcggc gctcgggcgc ctgagcgcga
     1561 gcatcgcgca cgagatccgc aaccccgtcg gcgcgatgag ccacgccggc cagttgctgc
     1621 gcgagtcgcc cagcctcacg cccgaggatc gggacctgac cgagatcatc gagaagaacg
     1681 gcgtgcgcgt gagccggatc atcgagaacg tgctgcagct ttcacgtcgg gacacgacgc
     1741 ggcaggagcg tttcgacgtc gtcgactgga tcggcacctt cgtgcgcgag tacgtcgaga
     1801 cgctgcagat cgacgccaac cgcttccaca tcgaggcgcc ttcgcacgga cggtccatcg
     1861 aagtcgagtt cgacccctcg cacctgcacc aggtcgtctg gaacctgtgc gacaacgcgc
     1921 tgcgacacgg tggcgccgag ggcgcctcgc acgtggaact gcacgccggg cgcatcgcct
     1981 cgaccgggcg gccgttcctc caggtctgcg accgcggtga aggcatcgac cccgcgcagg
     2041 ccgaacgcat cttcgagccg ttcttcacga ccggccacgg cggcaccggg ctgggactct
     2101 tcatttcgcg cgaactctgc cagaccaacg gcgcgctgct cgtctacgag ccgaggcccg
     2161 gcggtggtag cattttccgc gtcatttttg ccgacccgcg gcgatggaaa ggatgaacga
     2221 ccgcgccaga ctgtcgaggc cccgagccct ggtcgtcgac gacgagccgg acattcgcga
     2281 cctgctgtcg atgacgctgc gccgcatgca ggtcgacgtg gagaccgcga gcgaccgcgc
     2341 gagcgccatt cgtcggctcg gcagcgagcg cttcgacctc gtgctcaccg acatgcggct
     2401 gcccgacggc gacggcctcg acatcgtgca gtggatccag cagcagcgcc cgggcgtgcc
     2461 ggtggcggtg atcaccgcgc acggcaacgt cgaaacggcg gtgcgcgccc tcaagctcgg
     2521 cgccttcgac ttcatctcga agccactcga cctcgtcgcg ctgcgcaaga tgatccaggc
     2581 cacgctgaag ctgcgcgagg acgacaccgc gccgcacgat gagac
//
LOCUS       DASPFR010000193         6613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1031215, whole genome shotgun sequence.
ACCESSION   DASPFR010000193 DASPFR010000000
VERSION     DASPFR010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6613
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031215"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1787)
                     /locus_tag="VD737_04880"
     CDS             complement(<1..1787)
                     /locus_tag="VD737_04880"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HXW09927.1"
                     /translation="MDKSYSPRDIESRLYAAWEAAGWFAPAGQGAPYCIMIPPPNVTG
                     TLHMGHAFQHTLMDVLTRLHRMEGDRTLWQPGTDHAGIATQMVVERQLNAEGRHRRDL
                     GREAFVERVWQWKDESGGTISRQMRRLGDSVDWSRDRFTMDAGLSEAVIEVFVRLYEK
                     SLIYRGQRLVNWDPVLHTALSDLEVVSEPEAGQLWHLRYPLADGSGHLVVATTRPETM
                     LGDTAVAVHPDDERYRHLVGRQVRLPLADRSIPVIADEYCDPAFGSGCVKITPAHDFN
                     DYEIGKRHGLPMINVLTADAALNDDVPEPYRGLDRFEARKRVVADLEALGLLEKIEPH
                     TLPVPRGDRSGAVLEPWLTDQWYVRIAPLAEPAIAAVEDGRIRFVPDNWSKTYFQWMR
                     NIQDWCISRQLWWGHRIPAWYDADGNVYVARSESDAQRQATEKHGRAVALTRDEDVLD
                     TWFSSALWPFSTLGWPQPTAELKEFYPTSVLVTGFDIIFFWVARMVMMTTHFTGRVPF
                     RHVYINAIVRDAEGEKMSKSKGNTLDPLDLIDGIEFKNLLEKSTSGLLRREHKEKAEK
                     YVRSHFPSGIPAFGADALRFTFASLATFAR"
     gene            complement(1889..2185)
                     /locus_tag="VD737_04885"
     CDS             complement(1889..2185)
                     /locus_tag="VD737_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09928.1"
                     /translation="MGSQPSEDIPVLTDIVEPDGRSSKFIDDTALFLGELEAHLTAVI
                     HEQADELVHNACREMEALLLEQVSDRLKAELPGLIARVIADHFHGPRGNRVSGA"
     gene            complement(2213..2644)
                     /locus_tag="VD737_04890"
     CDS             complement(2213..2644)
                     /locus_tag="VD737_04890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HXW09929.1"
                     /translation="MPQVDFYPLGATDRRSRLVAACRLAEKAYEQGLKVAVRTASPAE
                     TAEFDDLLWTYADGSFVPHGVWPTDPDVAALTPVLVGNAALPESHRDVLINLAPDAPA
                     DFSAYARVCDVVGGDEEGRKAGRLRWRAYREAGCNPEAHPL"
     gene            complement(2669..4150)
                     /locus_tag="VD737_04895"
     CDS             complement(2669..4150)
                     /locus_tag="VD737_04895"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HXW09930.1"
                     /translation="MRLFVVTASPVRQKTDCAIVGLHDGGGLAGAGALIDAALKGRLT
                     RLVKAGDVRGKAGELRMVDTDSLPCARVLVVGLGKKGKFGRKQYRKALVAALGAIAKT
                     GARDAVSYLGEAVDPADDYYDARLAAEAFGAALYRVPAIRSSRKPPESALKAFGFAVR
                     ESGQKSQAQRGLRHGQAIAAGSALTRDLANLPANVCTPTYLAQQARELARSYPSVKAQ
                     VLEEADLKRLKMGSFLSVTNGTEEPAKLIVLRYDGGRAGAAPVALVGKGVTFDSGGIS
                     LKQPPGMDEMKFDMTGAASVFGAVKAAAEMGLALNLVGIVPACENMPSGRATKPGDIV
                     KSMSGQTIEIINTDAEGRLILCDALTYARRFKPAAVVDIATLTGACVIALGAHLSAVM
                     SNDDELAAELTAAGQRAEDRCWHMPLGEEYHEQLKSNFADFSNSCGREGGAITAACFL
                     AKFTEGLCWAHLDIAGTAYLTGANKGSTGRPVPLLMDFLIGRA"
     gene            4323..5450
                     /gene="lptF"
                     /locus_tag="VD737_04900"
     CDS             4323..5450
                     /gene="lptF"
                     /locus_tag="VD737_04900"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HXW09931.1"
                     /translation="MSLLNRLDRYLLREVASTFIAVTGVLLVVLMSNQFARVLGQAAQ
                     NDFAGPIVLTLLGLTTLQQLTLLVPIGLFLGIVLALGRLYHESEMTAMTACGVGPLRI
                     YKPIVLLSLVVAALLAVLSFRVVPAAWQKSQDLRVAALRAAQFGALEPGRFRSFAGGD
                     AVFYAERVGEQGELYDVFVQRNVGDKLEVAMAERAVQRGAGQAEQLFILYDGRRYEGV
                     PGAADWRIVEFREHGIPVQLPDTKKVKNREEMKSTPALLGSDAPRDRAELAWRTAVPI
                     MALALMVLAVPIARLRPRQGRFTRVGLAVLAYFLYSNLLAAARVWIEKDAPAGQLGLW
                     WVHLVPLAIAGLLLWQELHPGSRVTPADWWAAIRSRRKRVA"
     gene            5452..6525
                     /gene="lptG"
                     /locus_tag="VD737_04905"
     CDS             5452..6525
                     /gene="lptG"
                     /locus_tag="VD737_04905"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HXW09932.1"
                     /translation="MGVLGRYIIRTVLAYTGLVMLVLVALGALFLFIGQQDDIGVGNY
                     SSSQALMFVGLNLPSYLFELLPVAGLIGGLLGLGNLARGSELIAMRSAGVTTWRFCAW
                     LGVAGVMLAVLMFVVGEYVAPPLGQYARQMKVFAKFDEFSLAGNRATWVRDGDTIISV
                     DQQSASTRFGGIKVFQLGPERRLVSVALAESARVARDKVWRLDKYVETAFATDTLGSS
                     VLRNAEREVRTSLSPEFLGLAIAEPDAMGLRDLRSYILHLQRNDLQSARFETALWSRL
                     ARLAAVVVVVMLALPFSIGSLRNSGQGARTVIGVLIGAGFVLLSKTLESSGELFGLQP
                     WLVGWLPTLALAALTGTLLWRNR"
BASE COUNT          937 a         2239 c         2331 g         1106 t
ORIGIN      
        1 cgggcgaacg tcgcgagcga agcgaacgtg aaacgcagcg catccgcgcc gaacgccggg
       61 atgccgctcg ggaagtggct gcggacgtac ttctcggcct tctccttgtg ctccctgcga
      121 agcaatcccg aggtcgactt ctccagcaga ttcttgaact cgattccatc gatgagatcg
      181 agcgggtcga gcgtgttgcc cttcgacttc gacatcttct cgccctcggc gtcgcgcacg
      241 atggcgttga tgtagacgtg gcggaacggg accctgccgg tgaagtgggt ggtcatcatc
      301 accatgcgcg cgacccagaa gaagatgatg tcgaacccgg tcacgagcac ggaggttggg
      361 tagaactcct tcagttccgc ggtcggctgc ggccagccga gcgtcgagaa cggccagagc
      421 gccgagctga accaggtgtc gagcacgtcc tcgtcgcggg tcagcgcgac cgcgcggccg
      481 tgcttctccg tggcctgtcg ctgcgcgtcg gactccgagc gcgcgacgta gacgttgccg
      541 tcggcgtcgt accaggccgg gatgcggtga ccccaccaga gctggcggct gatgcaccag
      601 tcctggatgt tgcgcatcca ctggaagtac gtcttcgacc agttgtccgg cacgaagcgg
      661 atgcggccgt cctcgacggc cgcgatcgcg ggctcggcga gcggtgcgat gcgcacgtac
      721 cactggtcgg tgagccacgg ctcgagcacc gcgccggagc ggtcgccgcg cggcaccggc
      781 agcgtgtgcg gctcgatctt ctcgagcagg ccgagcgcct cgaggtcggc gacgacgcgc
      841 ttgcgcgcct cgaagcgatc gagcccgcgg tacggctccg gcacgtcgtc gttgagcgcc
      901 gcgtcggcgg tgagcacgtt gatcatcggc aacccgtggc gcttgccgat ctcgtagtcg
      961 ttgaagtcgt gggcgggcgt gatcttgacg cacccgctgc cgaacgccgg gtcgcagtat
     1021 tcgtcggcga tgaccggaat cgaccggtcg gccagcggca ggcgcacctg gcggccgacg
     1081 aggtggcgat agcgctcgtc gtcggggtgc accgccaccg ccgtgtcacc gagcatcgtc
     1141 tcgggccgcg tggtcgcgac gaccaggtga ccgctgccgt cggcgagcgg ataacgcagg
     1201 tgccacagct gcccggcctc gggctcggag acgacttcga ggtcggacag cgccgtgtgc
     1261 agcaccggat cccagttgac gagccgctgg ccgcggtaga tcagggactt ttcgtacagc
     1321 cgcacgaaca cttcgatcac ggcttcggac agccccgcgt ccatcgtgaa gcgatcgcgc
     1381 gaccagtcga ccgaatcgcc gagacggcgc atctggcgtg agatcgtgcc gcccgattcg
     1441 tccttccact gccacacgcg ttcgacgaag gcctcgcggc ccaggtcgcg tcgatggcgg
     1501 ccttcggcgt tgagctgccg ttccacgacc atctgcgtcg cgatgccggc gtggtcggtg
     1561 ccgggctgcc acagcgtgcg atcgccctcc atgcgatgca ggcgcgtgag cacgtccatc
     1621 aacgtgtgct ggaacgcgtg gcccatgtgc agcgtgcccg tcacgttcgg cggcgggatc
     1681 atgatgcagt aaggcgcgcc ctggccggcg ggcgcgaacc agccggccgc ctcccaggcg
     1741 gcatacaggc ggctttcgat atcgcggggg gagtagctct tgtccatcga cggcctcggg
     1801 cagaacggtc aagtataccg gcctgtccca gccgcgcccg cgcgaggcgg cgttccagcg
     1861 cccgtggtgc aggcgcccgg acgagccgct aggcccccga cacccggttc ccgcggggcc
     1921 cgtggaagtg atccgcgatg accctggcga tcaacccggg cagctcggcc ttgagacggt
     1981 ccgagacctg ctcgagcagc agcgcctcca tctcgcggca ggcgttgtgg acgagctcgt
     2041 cggcctgctc atggatcacc gccgtcaggt gggcttcgag ttcgccgagg aacagcgcag
     2101 tgtcgtcgat gaacttcgac gaacggccgt cgggctcgac gatgtcggtc aggaccggga
     2161 tgtcttcgga cggctggctg cccatcgctc gatcgtcact cctggtcggt cgtcacagcg
     2221 ggtgcgcctc ggggttgcaa ccggcctcgc ggtaggcgcg ccagcgcagg cggccggctt
     2281 tcctgccttc ctcgtcgccg ccgacgacgt cgcagacacg agcataagcg gaaaaatccg
     2341 cgggcgcatc gggcgccagg ttgatcagga cgtcacggtg cgactcgggc agcgctgcgt
     2401 tgcccaccag cacgggcgtc agcgcggcga cgtccggatc ggtcggccag acgccgtgcg
     2461 gcacgaagct gccgtccgcg tacgtccaca gcaggtcgtc gaactcggcc gtctcggccg
     2521 ggctcgccgt tcgcacggcc accttcaatc cctgctcgta ggccttctcg gcgagtcgac
     2581 aggcggccac gagtcgcgag cgacggtccg tcgcgccgag cggatagaag tcgacctggg
     2641 gcacggcgtc gcgcgaaagc cgacaggctc aggcgcgacc gatcaggaaa tccatcagca
     2701 acggcaccgg ccgaccggtg ctgcccttgt tggcccccgt caggtaggcc gttccggcga
     2761 tgtcgaggtg cgcccagcac aggccctcgg tgaacttggc gaggaagcag gccgccgtga
     2821 tcgcgccacc ctcgcggccg cacgagttgc tgaaatccgc gaagttgctc ttgagctgct
     2881 cgtggtattc ctcgccgagc ggcatgtgcc agcagcgatc ctcggcacgc tggcctgccg
     2941 cggtgagttc cgcggcgagt tcgtcgtcgt tgctcatgac cgcggacagg tgtgcgccca
     3001 acgcgatcac gcaggcgccc gtgagcgtcg cgatgtcgac gacggccgcc ggcttgaacc
     3061 gacgcgcgta ggtgagcgcg tcgcagagga tcagccgccc ctcggcgtcg gtgttgatga
     3121 tctcgatcgt ctgccccgac atgctcttga cgatgtcgcc gggcttggtc gcgcgaccgc
     3181 tcggcatgtt ctcgcaggcc gggacgatgc ccacgaggtt gagcgcgagg cccatctccg
     3241 cggccgcctt gacggcgccg aacacgctcg cggcaccggt catgtcgaac ttcatctcgt
     3301 ccatgcccgg cggctgcttg agcgagatgc cgccgctgtc gaacgtgacg cccttgccga
     3361 ccagtgcgac cggggcggcg cccgcacgac cgccgtcgta gcgcagcacg atcagcttcg
     3421 cgggctcctc ggtgccgttc gtgaccgaga ggaacgagcc catcttgagc cgcttgaggt
     3481 cggcttcctc caggacctgg gccttgacgc ttgggtacga gcgcgcgagc tccctggcct
     3541 gctgcgcaag gtaggtcggc gtgcagacgt tggcgggcag gttggcgagg tcccgcgtga
     3601 gcgcgctgcc ggccgcgatc gcctggccgt gcctgaggcc gcgttgggcc tgcgacttct
     3661 gcccggattc acgcacggcg aaaccgaacg ccttgagcgc ggactccggc ggcttgcgcg
     3721 agctgcggat cgcgggcacg cggtagagcg ccgcgccgaa cgcctccgcg gcgagtcgcg
     3781 catcgtagta gtcgtcggcc gggtcgacgg cctcgccgag gtagctcacc gcatccctgg
     3841 caccggtctt tgcgatcgcg ccgagtgcgg ccacgagcgc cttgcggtac tgcttgcgac
     3901 cgaacttgcc tttcttgccg agccccacga cgagcacgcg cgcgcagggg agggagtcgg
     3961 tgtcgaccat gcgcagttcg ccggccttgc cgcgcacgtc gcccgccttg accagccgcg
     4021 tcagccgccc cttgagcgcc gcgtcgatca acgcgcccgc gccggcgagg ccgcctccgt
     4081 cgtgcagacc gacgatggcg caatcggttt tctggcgcac gggcgaggcg gtgacgacga
     4141 ataggcgcat gaatcgacgg tcccttccag ggggcggtcg aaccgctgcg cgatgcgcgt
     4201 atgcggcgct cgaatcgccc gttaaactgc gacgctgcgc atctgttctc gcgtcgggcc
     4261 aagcccgctc gcggcgtgcg cgtagcccgc gagtgtaccg attcccgccg aggcccgcga
     4321 atttgtcgct gctcaatcgc ctggaccgct acctgctgcg cgaagtggcc tcgaccttca
     4381 ttgccgtcac gggcgtgttg ctcgtcgtgc tgatgtcgaa ccagttcgcg cgcgtgctcg
     4441 gccaggccgc gcagaacgac ttcgccgggc cgatcgtgct cacgctgctc gggctcacca
     4501 cgctgcagca gttgacgctg ctggtgccga tcggactctt tctcggcatc gtgctcgcac
     4561 tcggccggct ctatcacgag agcgagatga cggccatgac cgcctgcggc gtcgggcccc
     4621 tgcggatcta caagccgatc gtgctgctga gcctcgtggt cgccgcgctg ctcgcggtgc
     4681 tgtcgttccg cgtcgtcccg gccgcctggc agaagtcgca ggacctgcgc gtcgccgcgc
     4741 tgcgggccgc gcagttcggc gccctcgagc cgggccggtt ccgcagcttc gcgggcggcg
     4801 acgcggtgtt ctacgccgaa cgtgtcggcg agcagggcga gctctacgac gtgttcgtgc
     4861 agcgcaacgt cggcgacaag ctcgaagtcg cgatggccga gcgcgcggtg cagcggggcg
     4921 caggacaggc cgaacagctg ttcatcctct acgacggccg ccgttacgag ggcgtgccgg
     4981 gtgcagcgga ctggcgcatc gtcgagtttc gcgagcacgg catcccggtg cagttgccgg
     5041 acaccaagaa ggtcaagaac cgcgaggaga tgaagtcgac gcccgcgctg ctcggctccg
     5101 acgcgccgcg cgaccgcgcg gaactcgcct ggcgcacggc cgtccccatc atggcgctcg
     5161 ccctgatggt gcttgccgtg ccgatcgcgc gcctgcggcc gcggcagggt cgcttcacgc
     5221 gtgtcggtct cgcggtgctc gcctacttcc tctattcgaa cctgctcgcc gcggcgcgtg
     5281 tgtggatcga gaaggacgca ccggccggac agctcgggct ctggtgggtg cacctcgtgc
     5341 cgctcgcgat cgccgggctg ctgctctggc aggagctgca cccgggcagc cgcgtgacgc
     5401 ccgcggactg gtgggcggcg attcgttcgc gacgcaagcg ggtcgcctga cgtgggcgtg
     5461 ctcggccgct acatcatccg cacggtgctc gcgtacacgg gcctcgtgat gctcgtgctg
     5521 gtggcgctcg gcgcgctctt cctgttcatc ggccagcagg acgacatcgg cgtcggcaac
     5581 tacagctcga gccaggcgct gatgttcgtc ggactcaacc tgccgagcta cctgttcgag
     5641 ctgctgcccg tggccggcct gatcggcggc ctgctcggcc tcggcaacct ggcgcgcggc
     5701 agcgagctga tcgcgatgcg gtcggcggga gtgacgacgt ggcgcttctg cgcgtggctg
     5761 ggagtggccg gtgtgatgct cgcggtgctg atgttcgtgg ttggcgagta cgtcgcgccg
     5821 ccgctcggac agtacgcccg gcagatgaaa gtgttcgcca agttcgacga gttcagcctc
     5881 gccggcaatc gggccacctg ggttcgcgat ggcgacacga tcatcagcgt cgaccagcaa
     5941 tcggccagca cgcggttcgg tggcatcaag gtgttccagc tcgggccgga acgtcggctg
     6001 gtcagcgtcg cactcgctga atcggcccgc gtcgcacgcg acaaggtgtg gcggctcgac
     6061 aagtacgtcg agactgcatt tgccacggac acgctgggct cgtccgtgct gcgcaacgcc
     6121 gagcgcgagg tacgcacgtc gctgtcgccg gaattcctcg gcctcgccat cgccgaaccg
     6181 gacgccatgg gcctgcgcga cctgcggtcg tacatcctgc acctgcagcg caacgacctg
     6241 cagtcggcca ggttcgaaac ggcattgtgg tcgcgcctgg cgcggctcgc ggcggtcgtc
     6301 gtcgtcgtga tgctggcgct gccattctcg atcggctcgt tgcgaaactc ggggcagggt
     6361 gcccgcacgg tgatcggcgt gctgatcggc gcgggcttcg tgctgctcag caagacgctc
     6421 gagagcagtg gcgagctgtt cggcctgcag ccgtggctgg tcgggtggct accaacgttg
     6481 gcgctcgcgg cgctcaccgg gacgctgctg tggcgcaatc ggtgaatccg cacctctccc
     6541 gcgccggcgg gagaggtcgg cagaaggcct ggtgggggca ggtggcctga tcgctggaag
     6601 tgtcggtgct gcg
//
LOCUS       DASPFR010000194         3855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_543002, whole genome shotgun sequence.
ACCESSION   DASPFR010000194 DASPFR010000000
VERSION     DASPFR010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543002"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3855)
                     /locus_tag="VD737_04910"
     CDS             complement(<1..>3855)
                     /locus_tag="VD737_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXW09933.1"
                     /translation="DDAECVVVGLGSVTDDAEAVAAHLRTQGRKVGVVSVKLLQPFPE
                     AELVQALKGKKAVTVLERSETTALTSFVTQALFKGVENASNGIRHPGIPALHALPKIT
                     TAIFGLGAHDLQPRHLVAAFRNMDTRNVPFVYLGSQFFAKNPPPQLAALQARLRAAYP
                     ETESMALETEPNPVLLPPEAFRVRFHSIGGYGTIATGKLLTDILAGVLGLHSKAAPKY
                     GSEKSGAPTNYYITLSPEPVKITNAELEDVEIVISPDHRVFGHTNPLRGLVDGGTFIL
                     QSSLSPLEAWKELPEHARRTLRAKKVKFFVLDGFAVAKRHAPTPELETRMMGIAFIGA
                     VCGHVDRIKAGASEGALLEKIRSQIAKKFGAKGGAVVEGNLAVMREGLAATRRVDYET
                     AAFAAVDTQPPTRPKRSVAISAGMCRASSASPDGGFLDRDYYEQMIAAPFREGTIAEA
                     PVLPGAGLFMPAGTAAWKDKGLFRREVPQFVAERCTGCMECTLVCPDAAIPNTVHDIH
                     DLLQSGIRELDLPEAQRAELLAQVYPLTEAVRARYKATRGSLELHDAVADAATSLDGT
                     NANVRRSVQRLAEALRRFPVARTRPFFDAPEKECAGSGGLYSVAIDPWKCSGCLECID
                     VCGPGALEPRVQDDVLLDDLQVRFERLSRTGNTPARFVRDAVRPGGDIKRLMLDRANY
                     YATTGGHGACRGCGEVTAIRLVTSMNHAIQKSRRQEHVAELTSLLERLRAKHASLEEG
                     EAGRRERIASTIATLEKRLYLLESGPTGNGPACAVIANATGCSSVYASTFPFNPYNDP
                     WVNSLFQDTPAVAKGLFEGLTANALDDVKALRIARLELDDAYDPASHDRQFRTFGWTE
                     FTPQELALLPTAFSIGGDGATYDIGFGALSRLLTTRTPIKVMVVNTGVYSNTGGQAST
                     ASLTGQDSDLTRFGAAHAGKQEDRKELGLLAAFHPNVFVVQTSTALQGHFLGNVMEYL
                     NYTASPAVLDVYTPCQAEHGIADAAASRRARLAVESRMNPVFVHDPRRGATLHARFSL
                     DGNPALDRDWAMGTLTYVEDGTTRLLETPLTPADFAREETRFKKQFRKLPPAAEANAL
                     PIHEYVDLPVAARDGKVPFVWSTDDERRLEKLEASPTIVHLVEERRRYWRTLQHLAGR
                     HVEQLDAEHRSELATLEARYQESVAERESSLDSIARAMSELAAASNAPPAAMATVLAP
                     FGGGAATASTRDGVSGASTSPRSAGTASVGGLKTIREEDLAACVNCKTCYQQVPELFE
                     KATIVVDGKAMEVGRVIPGVLER"
BASE COUNT          530 a         1327 c         1359 g          639 t
ORIGIN      
        1 cgctccagga cgcccgggat cacgcgcccg acttccatcg ccttgccgtc gaccacgatc
       61 gtcgccttct cgaacagttc cggcacctgc tggtagcagg tcttgcagtt cacgcacgcg
      121 gccaggtctt cctcgcggat cgtcttcaag ccgccgacgg aagcggtccc ggcagacctg
      181 ggcgacgtcg acgcgccact cacgccgtcc cgggtcgatg cggtggccgc gccgccgccg
      241 aacggcgcca gcaccgtcgc catcgccgcc ggtggagcgt tcgaggctgc cgccagctcg
      301 ctcatcgcgc gcgcaatcga atcgagcgac gactcgcgct ctgccaccga ctcctggtac
      361 cgcgcctcca gcgttgcgag ctccgagcgg tgctcggcgt cgagttgctc gacgtgccgg
      421 ccggcgaggt gctgcagcgt gcgccagtag cgacgacgct cctcgaccag gtggacgatc
      481 gtcggcgacg cctcgagctt ctcgagtcgc cgttcgtcgt ccgtggacca gacgaacggc
      541 accttgccgt cgcgggcagc gacgggcaaa tccacgtatt cgtggatcgg cagtgcgttc
      601 gcctcggccg ccggcgggag cttgcggaac tgcttcttga accgcgtctc ctcgcgcgcg
      661 aagtcggccg gcgtcagcgg cgtttcgagc agcctggtcg tgccgtcctc gacgtaggtg
      721 agcgtcccca tcgcccagtc cctgtcgagc gccgggttgc cgtccagcga gaaccgcgcg
      781 tgcagcgtgg cgccgcgacg cgggtcgtgc acgaagaccg ggttcatgcg gctctcgacc
      841 gcgaggcggg cgcggcggct ggccgcggca tcggcgatgc cgtgttccgc ctggcagggc
      901 gtgtagacgt cgagcaccgc cggcgacgcc gtgtagttga ggtattccat gacgttgccg
      961 aggaagtgcc cctgcaacgc ggtggaggtc tgcacgacga agacgttcgg gtggaacgcc
     1021 gcgagcagcc cgagttcctt gcgatcctcc tgcttgcccg cgtgcgcggc gccgaagcgg
     1081 gtcaggtcgg agtcctggcc ggtcaggctc gccgtcgagg cctggccgcc ggtgttcgag
     1141 tacacgcccg tgttgaccac catcaccttg atcggcgtgc gcgtcgtgag caggcgcgac
     1201 aacgcgccga agccgatgtc gtacgtggcg ccgtcgccgc cgatgctgaa cgccgtcggc
     1261 agcagcgcga gttcctgcgg cgtgaactcg gtccagccga acgtgcggaa ctgccggtcg
     1321 tgactggccg ggtcgtacgc gtcgtcgagt tcgaggcgtg cgatgcgcag cgccttgacg
     1381 tcgtcgagcg cgttcgcggt caagccttcg aacaggccct tcgcgacggc cggggtgtcc
     1441 tggaacaggc tgttgaccca cgggtcgttg taggggttga acgggaaggt cgacgcgtag
     1501 acgctcgagc agccggtcgc gttcgcgatc acggcacagg ccgggccgtt gccggtcggg
     1561 ccgctttcga gcaggtacag ccgcttctcg agcgtcgcga tcgtcgacgc gatgcgttcc
     1621 cggcggcccg cctcgccttc ctcgagcgac gcgtgcttcg cgcgcaggcg ctcgagcagg
     1681 ctggtgagtt ccgccacgtg ctcctggcgc cgcgacttct ggatcgcgtg gttcatcgag
     1741 gtgacgaggc gtatcgccgt gacctcgcca cagccccggc aggcgccgtg gccgcccgtc
     1801 gtcgcgtagt agttcgcgcg atcgagcatc agccgcttga tgtcgccgcc tggccgcacg
     1861 gcatcgcgca cgaagcgcgc cggggtgttg cccgtgcggc tcaggcgctc gaagcgcacc
     1921 tgcaggtcgt cgagcagcac gtcgtcctgg acacgcggct cgagcgcacc cggaccgcag
     1981 acgtcgatgc actcgagaca gccgctgcac ttccacgggt cgatcgcgac ggaatacagg
     2041 ccgccgctgc cggcgcactc cttctccggt gcatcgaaga acggccgcgt ccgtgccacc
     2101 gggaatcgcc gcagcgcctc ggccaggcgc tgaacgctgc gacgcacgtt ggcattggtg
     2161 ccgtccaggc tcgtggcggc gtcggcgacc gcatcgtgca gttcgagcga tccgcgcgtc
     2221 gccttgtagc gcgcgcgcac ggcttccgtc agcgggtaca cctgtgcgag cagttccgca
     2281 cgctgcgcct cgggcaggtc gagttcacgg atgccggact gcagcaggtc gtggatgtcg
     2341 tgcacggtgt tcgggatggc cgcgtcgggg cagacgagcg tgcactccat gcagcccgtg
     2401 caacgctcgg cgacgaactg cggcacctcg cgccggaaca atcccttgtc cttccaggcc
     2461 gccgtgccgg ccggcatgaa cagcccggcg cccggcagca ccggcgcctc ggcgatggtg
     2521 ccttcgcgga acggcgccgc gatcatctgt tcgtagtagt cgcggtcgag gaacccaccg
     2581 tcgggcgagg cgctcgaggc ccggcacatg ccggcggaaa tcgcgacgct gcgcttcggc
     2641 cgcgtcggcg gctgcgtgtc gaccgcggcg aacgcggccg tctcgtaatc gacgcgtcgc
     2701 gtcgcggcga ggccctcgcg catcacggcg aggttgcctt cgacgaccgc cccacccttc
     2761 gcgccgaact tcttcgcaat ctggctgcgg atcttctcga gcagcgcgcc ctccgacgcc
     2821 ccggccttga tgcgatcgac gtggccgcag acggcgccga tgaacgcgat gcccatcatg
     2881 cgcgtctcga gttcgggcgt cggcgcgtgg cgcttggcga ccgcgaaacc gtcgagcacg
     2941 aagaacttga ccttcttcgc acgcagcgtg cgacgcgcgt gctcggggag ctccttccag
     3001 gcctcgagcg gcgacaggct cgactgcagt atgaaggtgc caccgtccac gaggccgcgc
     3061 agcgggttgg tgtgcccgaa cacgcggtgg tccggcgaga tgacgatctc gacgtcctcg
     3121 agctcggcat tggtgatctt gaccggctcg gggctcagcg tgatgtagta gttggtcggc
     3181 gcgccgctct tctccgagcc gtacttcggc gcggccttcg agtgcagccc gagcactcct
     3241 gccaggatgt ccgtgagcag cttgccggtc gcgatcgtgc cgtagccgcc gatcgagtgg
     3301 aaccgcacgc ggaacgcttc cggcggcagc agcacgggat tcggctcggt ttcgagcgcc
     3361 atcgactcgg tctcgggata ggcggccctg aggcgcgcct gcagtgccgc gagctgcggc
     3421 ggcgggttct tcgcgaagaa ctggctgccc aggtacacga acggcacgtt gcgcgtgtcc
     3481 atgttgcgga acgcggccac caggtgacgc ggctgcaggt cgtgtgcgcc gaggccgaag
     3541 atcgcggtcg tgatcttggg caatgcgtgc agcgcgggga tgccggggtg acggatgccg
     3601 ttgctcgcgt tctcgacgcc cttgaacagc gcctgcgtga cgaagctggt cagggcggtg
     3661 gtctcggagc gctccagcac cgtgacggcc ttcttgccct tgagcgcctg caccagttcg
     3721 gcttcgggga acggctgcaa tagcttgacc gagacgacgc ccaccttcct gccctgcgtg
     3781 cgcaggtgcg cggcgaccgc ttcggcgtcg tcggtgaccg agccgaggcc gacgaccacg
     3841 cattcggcgt cgtcg
//
LOCUS       DASPFR010000195        16745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1520592, whole genome shotgun sequence.
ACCESSION   DASPFR010000195 DASPFR010000000
VERSION     DASPFR010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16745
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520592"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1538)
                     /gene="iolC"
                     /locus_tag="VD737_04915"
     CDS             complement(<1..1538)
                     /gene="iolC"
                     /locus_tag="VD737_04915"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: protein motif:HMM:TIGR04382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HXW09934.1"
                     /translation="MAEAAALDVVCLGRAAVDLYGQQVGGRLEDVQSFAKYLGGSSAN
                     LAVGLARLGLRPAMLTRVGDEHLGRFVREQLAREGVDVSHVVTDPERLTGLVVLGIAG
                     ADAYPHVFFRTLCADMGLVTDDFDEAWISSARALAVTGTHLSTSTTRRAVQQAMQWAR
                     SHGRGVVLDIDYRPVLWGLTTAGAGDNRYVAAQAVTQVLQEFLADCDVIVGTEEEVRI
                     AGGSDDTDAALRSIRERSNAVIVLKRGAAGCVIHEPGVEPRAVAGFPVEPFNVLGAGD
                     AFLSGLLAGWLEGAGWERCAELGNACGALVVSRHGCSPAMPSRVELEAYLARASSLRR
                     PARDEWIAHLHRATTTRRPRDELHVLAFDHRRQFETLAGQYGAPWTRISQFKDLVARA
                     VERVAERDGGARVGVIVDERHGNAALERFTHRGTWVGRPIELPGSKPLRFEPLDEPGL
                     ALRDWPAHHVVKCLAFYHPDDPGDLRRDQEARLAALHADCVALDRELLLELITTSSGR
                     PNDDR"
     gene            complement(1531..2361)
                     /locus_tag="VD737_04920"
     CDS             complement(1531..2361)
                     /locus_tag="VD737_04920"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HXW09935.1"
                     /translation="MTTNTLHLTDHRTGFGHGETVITRHDAEDDPVGLGVAVLRLRAG
                     ERLEQPADVETAWLLMHGHVRGTAGDRRFEFQRRSLFDESASCIHVCTGTSVTLDAVT
                     DAELTVYRCPNRRVFEARVFGPGDVPDEHRGAGQVRDRCLRLVRTIFDRKNSPDEVEL
                     VLGEVVTLPGGWSSYPPHHHPQPEIYHYRFTEPQGFGHAELGEAVVKVRQYDTVRIPA
                     GHDHAQCAAPGYGMYYSWVIRHLPDRPYTVPEFTDEHAWTMRSDAQFWWPAGARDRDG
                     "
     gene            complement(2358..3518)
                     /locus_tag="VD737_04925"
     CDS             complement(2358..3518)
                     /locus_tag="VD737_04925"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXW09936.1"
                     /translation="MTAARIGLIGTGYIGRAHAIALSTVGTVFADVEPPVLELVADTD
                     PDRAARAARELGFRRSTGDWRALVADPDVDVVVVCTPNHLHRDMALAAIAAGKHVSCE
                     KPLALSATDARQMASAADVAGLCHLVGFNYACNPLLQTARELVGAGEIGTPIGFHGRY
                     FEDYLSDPGLPFTWRCERALAGSGALADLGTHLINLAHVLLGDLGRVMARLSTVIGER
                     REATTGRARQVENEDTAQVLVEFANGVRGSFDVSRVATGYKCGLAFDLFGTRGSLRFD
                     QERMNELQLYRNDDRPGLRGFRTILAGPEHADYRSFCPAPGHGLGINDLKVIESRNLL
                     AAIARRRVDPRARGATPDFHEGARVQAVIEAIERSHATQAWIDVEAHGGAGA"
     gene            complement(3515..4537)
                     /gene="iolG"
                     /locus_tag="VD737_04930"
     CDS             complement(3515..4537)
                     /gene="iolG"
                     /locus_tag="VD737_04930"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HXW09937.1"
                     /translation="MAASRDPLRVALIGAGRIGRIHADNLTRTPGARLSCVCDADEGA
                     AESLAAQRDAVVASVDAALADPAVDALLIASPTDTHADLIEAGARAGKAVFCEKPLDL
                     DLARARRCVEAAERAGIVLRLGFNRRYDPSFARVKRELAGGAIGPLATLTITSRDPAP
                     PPIDYVRRSGGLFRDMMIHDFDMARWLLAEEPVEVYARGGCFVDPEIAAAGDVDTAAV
                     LLVTASGRLCQISNSRRCSFGYDQRIEAFGATGMLRANNATATHVEHASAAGFTTEPA
                     LPFFLERYAEAYRRELVDFVRACGGESVELATALDGLRALELADAAQRSHETRQPVRV
                     EPRTLQ"
     gene            complement(4539..5387)
                     /locus_tag="VD737_04935"
     CDS             complement(4539..5387)
                     /locus_tag="VD737_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HXW09938.1"
                     /translation="MKAPKNPESLRNEIVRRYENLSPRLKQVAKFVLENPNDVALQTL
                     AVIAARSKVQPSTIVRFAKTFGYEGASDIQKLFRDEILNHAPSSNYSERIRQYSEGSG
                     AASTRGAHDLMREFADNNILALRHLKEVVRDADLERSLDLIEAARAVYIVGMRRSFPV
                     AAYLAYALRHVEKRAYLIDGVAGMLTEQASIVEAEDLMIAISFHPYARETAEVVAAAR
                     ERNAPVIAISDSRVSPIAREAELCFEVKDAEVRQFRSLTASLCLAQTLAISYAYRLGT
                     RRPRDH"
     gene            complement(5482..8211)
                     /locus_tag="VD737_04940"
     CDS             complement(5482..8211)
                     /locus_tag="VD737_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXW09939.1"
                     /translation="MTDSSTGRPLPNSTVRIEPLGRTVTADRSGEFRVADVPAGSYQL
                     TVTSVGFREATQTLEVPATGAATQTFALLGAGVEEITVTGFRLALATALQDKKSSAVI
                     KDSITADDAGKLPDQNAAEALQRLPGVSTTTDQGEGRYVTVRGIDPGLNNVTLDNQIL
                     GTPEPDDRRIALDTVPANLLAKLEVVKSVTPDMDGNAIGGSINIVTPSAFDQEDGHFA
                     SASMEYGYYDLNGENPFGGALAWGQTFGEDRWGIVLSGSYSEREYASHNLQGGDPWQE
                     EGDFFVPDELVLRDYVIERKRSGVVANLEFRPSDTTKLYFRNLYNRFEDIELQPETIY
                     DYRNGDLEDQTATSGTFTEGEGSRELQSRREIQSIQSSTLGGDFGFGSGWTLGASYTL
                     GKSEQDTPWDYGWVFELDDALPMTYDTSGEFFDVTAGPEFQDTEGYEFDEHFRAKQMV
                     EEDLNIAQLDLRRDLSFGGRSGFVKFGAKLLDREKTSDQSATVYDGFDSDDDFLLSQV
                     ARSGDPDFYCSERCYTFGPKINFEAAQRFFRDNQAAFEVNDGDTVAEEYGVDYVIDEK
                     ISAGYLMLQLDVTPTLSLTGGVRVEKTEADYQGYDLEFVDGDIDPVPPKVTGSKDYTN
                     WLPGVQMRWAPRQELVVRAAWTNTIGRAGYEQLVPFRSFEIEELDPEDCSNAPDPCFE
                     GALETGNSDLEPIESMNWDVSVEWYLPAGVIATGVFFKDLENPVFTRFQVLEEEEFEG
                     RFYQELEVLRPENAESGDIFGIEFNYQQSFTGLPAPFDGLGFSLGYTYSDSSAEVFDR
                     DDDIPFFLQSEHVGNLALYYERAGVGLRLGYSYRSEYLDTLGEGPDEDLYIDDHGQLD
                     FKASYDFTDMFTVYLQMQNLTDEPLRYTSGRGRRLAENEIYSWNAMAGVQVKF"
     gene            complement(8520..10052)
                     /locus_tag="VD737_04945"
     CDS             complement(8520..10052)
                     /locus_tag="VD737_04945"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004951467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HXW09940.1"
                     /translation="MNAPRPTAVPTPDTFELGHFVEGREAAGTSGRRGAVHDPSTGAL
                     RGHVAFASEAETQAAIAAAAAALPAWSATPPLQRARVLFRYKALLESHLDELARLITS
                     EHGKVFSDARGEVIRGIEVVEFACGIPQMLKGEFSESVGTGVDSWSQRQPVGVCAGIT
                     PFNFPAMVPMWMFPVAIACGNTFVLKPSEKDPSCPMRLAQLLKEAGLPDGVFNVVNGD
                     REAVDTLLTDPRVGAVSFVGSTPVAEYIYRTASAHGKRVQALGGAKNHMVVMPDADTA
                     NAASALIGAAYGSAGERCMAISVAVAVGDAAEPLIEAVRAQLAQLKVGSGLADGVEMG
                     PLVTREHLERVRGYVELGVQEGANLVVDGRSLRIKGHEGGYFLGPCLFDAVEPRMRIY
                     REEIFGPVLCVVRVPDLGAALELVNGHEFGNGTAIFTASGEAARTFTQGVQAGMVGIN
                     VPIPVPMAFHSFGGWKRSLFGALHVHGPDGVRFYTRLKTVTSRWPTSRREGAQFVMPT
                     MK"
     gene            complement(10211..11056)
                     /gene="phhA"
                     /locus_tag="VD737_04950"
     CDS             complement(10211..11056)
                     /gene="phhA"
                     /locus_tag="VD737_04950"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HXW09941.1"
                     /translation="MSAPPATYGASSHGLRGDYRDMRPDYTVTQDYSAYDDAAQQRWR
                     RLYQRQIKLVPQRACDEFLRVIETLDYGAGIPRFEQVNERLGRATGWEVVAVPGLLPE
                     PVFFGHLAARRFPVTVWLREEAEFDYIVEPDVFHDFFGHVPLLFDPVFADYMQAYGRG
                     GQKAEGLHALEYLARLYWYTVEFGLIRGRDGLRIYGAGILSSPSEIVHALDSPQPRRI
                     GFDLRRVMRTKYKIDTFQPTYFVIDGFDQLFRETAPDFTPLYTEVAQQETLPADALLE
                     GDRVY"
     gene            11216..11596
                     /locus_tag="VD737_04955"
     CDS             11216..11596
                     /locus_tag="VD737_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09942.1"
                     /translation="MKLWTALAIAPFMMATGALAEAACVYPQPPQALPNGGSATKEEM
                     LAAQGLVKEYVNNVQGTYLPCLEKERDEATGALDNMDPEYTAKKGSIEAIHAKKHNAA
                     LDELQAFVDRWNAEKKAFTAKSDK"
     gene            11643..12842
                     /locus_tag="VD737_04960"
     CDS             11643..12842
                     /locus_tag="VD737_04960"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HXW09943.1"
                     /translation="MPSPAEAEALIRARVQPLPVESRPLASLAGAVLAQAVRSERDQP
                     PFDRVMMDGIALASQAWRAGRRRYRIAGTQAAGLPPRRLEDPETCLEAMTGAVLPPGC
                     DCVVPVEHITVEDGVAVVDEVTPVEPFWNVHTRGLDCREGDMLLTRGTVLGAPELAVL
                     ASAGLPRADVHADPRIVIVATGDELVAPDEPIEAWQVRRSNSYALRGALHLRGFHRVA
                     EDHLPDDHAVLAQRLATHLATHDVVLLTGGVSMGRFDHVPSVLRELGVAEVFHKVAQR
                     PGKPIWFGIGPAGQTVFGLPGNPVSSLVCLLRYAVPAIYAMLGAKPRPPEQVPLGAGH
                     LVKAGLWHYLPVRVEQSPALGVVAMPRPTRGSGDFVSLLGTDGFVELPPGPREMPAGC
                     VVAFYRW"
     gene            12874..13914
                     /gene="moaA"
                     /locus_tag="VD737_04965"
     CDS             12874..13914
                     /gene="moaA"
                     /locus_tag="VD737_04965"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HXW09944.1"
                     /translation="MTSVTTVPIALHDRLGRPIHDLRISVMDRCNFRCPYCMPQATFH
                     EKYRFLKSAERLTFEEIARLARLAAGLGVRKLRLTGGEPLLRTGLPELVAELSTIEGV
                     EDLALTTNGVLLAQHAAELKGNGLHRVTVSLDSLDDAVFTAMSGGYGGLAQVLQGIDA
                     ALESGLKPVKVNTVVQRGRNDHTVLDVLEFFRGKDVTVRLIEYMDVGTRNHWDPGDVV
                     PSAELLDRIRARWSVSPAPGRYRGEVAERYVYDDGAGEIGFVSSVSAPFCGDCNRARL
                     SSEGVLYTCLFASQGVDLRSPLRAGATDVELLDLLRRAWSQRADRYSELRGELRTREA
                     PLRKVEMNYIGG"
     gene            13920..14393
                     /gene="moaC"
                     /locus_tag="VD737_04970"
     CDS             13920..14393
                     /gene="moaC"
                     /locus_tag="VD737_04970"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HXW09945.1"
                     /translation="MSALTHVDSSGRPTMVDVGAKAVTHRTAVAETRVRFPAAVAAAL
                     RDAQFATAKGPVFHTAIVAGVMAAKRTHELIPFCHPLGLERCDVAIDMQGDEAVIRCT
                     VSIHHKTGVEMEALTGASVAALTVYDMCKALSHAIVIAETRLVEKHGGKRDVTAA"
     gene            14427..14672
                     /locus_tag="VD737_04975"
     CDS             14427..14672
                     /locus_tag="VD737_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXW09946.1"
                     /translation="MANRIHLQYFAVLREQAGRSEETLETEALTPAELYEQVRASHGF
                     SLPRAMLRVAVNDEFRDWTVPLASGDRVVFIPPVAGG"
     gene            14672..16258
                     /locus_tag="VD737_04980"
     CDS             14672..16258
                     /locus_tag="VD737_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HXW09947.1"
                     /translation="MQPFSFSDSPIDAAAGRAALADPACGGYASFEGWVRDHNEGRQV
                     RHLEYEAFATLAVREGERIVAEAIEKYGVSRAACVHRVGDLALGEMAVWVGVSSPHRG
                     EAFAACRYIIDEVKHRVPIWKKEHYVDGDSGWVNCERCAQPSAHEHEHGQAHQPDHAH
                     GHEHVHATGSRPDTRVPHGVPDYSRQMALPEVGRDGQARLRQGSVLVIGAGGLGVPVL
                     QYLAAAGVGRIGIVDGDVVEASNLHRQPLYGWADLGAKKARVAAERLRALNPDVRVET
                     YDERATPANISDWLARYDLVVDCTDNFATKFLVNDAAVSLGKPAVFASIYQYEGQLQT
                     YLPRPDWPCLRCLWPEAPRDGLVGNCAEAGVLGAVPAALGAMQAMQALKLLLGLPADA
                     RPALHTFDLLGMHWHTLKAARNPACDHDTRVLQAADAEDDELEREYPDLRAASASGLT
                     LVDIREPWERASDDPARQIPMHVPLSAFVNGEAALPEGRYLLVCAHGVRSHALAEHLR
                     SHGHREVYSLAGGLAGLAGV"
     gene            complement(16255..16677)
                     /locus_tag="VD737_04985"
     CDS             complement(16255..16677)
                     /locus_tag="VD737_04985"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXW09948.1"
                     /translation="MAVVVRVATVEDIPDMHRVRQSVIENRLASAAITAASYVPEIQT
                     RGRGWVAVEDGQVLGFAVGNRENGNIWALFVTPAAEGRGVGRRLHDEMVAWLFDQGCR
                     RLWLSTEPGTRAQRFYESAGWQFRQHLPGGEAMYELLP"
BASE COUNT         2525 a         5810 c         5783 g         2627 t
ORIGIN      
        1 cggtcgtcgt tcgggcgccc actcgacgtc gtgatcaact cgagcagcag ttcgcggtcg
       61 agcgcgacgc aatccgcgtg cagggccgca agacgtgctt cctggtcccg ccgcaaatcg
      121 ccgggatcgt ccgggtggta gaacgccagg cacttcacga cgtggtgtgc cggccagtcg
      181 cgcagcgcga ggccgggttc gtcgagcggc tcgaatcgca gcggcttcga tcccggcaat
      241 tcaatggggc ggccgaccca ggtgccgcgg tgcgtgaagc gctcgagtgc ggcgttgcca
      301 tgacgctcgt cgacgatcac gccgacgcgc gcaccgccgt cgcgttcggc gactcgctcg
      361 acggctcgcg ccacgaggtc cttgaactgt gagatgcggg tccacggcgc gccatactgg
      421 ccggccagcg tctcgaactg gcgacggtgg tcgaacgcca ggacgtgcag ctcgtcgcgc
      481 ggacggcggg tcgtcgtcgc gcggtggagg tgggcgatcc actcgtcgcg ggcgggacgc
      541 cgcagggacg atgcccgtgc gaggtacgcc tcgagttcga cgcgcgacgg catggccggc
      601 gaacacccgt ggcggctcac taccagcgca ccgcaggcat tgccgagttc cgcgcagcgc
      661 tcccagccgg cgccttcgag ccagcccgcc agcaagccgc tcaggaatgc gtcgccggcg
      721 cccagcacgt tgaacggctc gaccggaaag cctgccaccg ctcgcggctc gacgccgggc
      781 tcgtggatca cgcagcccgc cgcccctcgc ttcagcacga tcacggcgtt cgaccgctcg
      841 cggatgctgc gcagggccgc gtcggtgtcg tccgagccgc cggcgatgcg cacctcttct
      901 tcggtcccga cgatcacgtc gcagtcggcg aggaactcct gcagcacctg cgtcaccgcc
      961 tgcgcggcga cgtagcgatt gtcgccggcg cccgcggtgg tcagccccca cagcacggga
     1021 cggtagtcga tgtcgagcac gacgccgcgg ccgtgcgagc gtgcccactg catcgcctgc
     1081 tgcacggcgc gccgcgtcgt ggaggtcgaa aggtgcgtgc ccgtcacggc cagcgccctc
     1141 gccgaggaga tccacgcctc gtcgaaatcg tccgtgacga gacccatgtc cgcgcacagc
     1201 gtgcggaaga agacgtgcgg ataagcgtcg gcgccggcga tgccgagcac gaccaggccg
     1261 gtgagtcgtt cgggatcggt gacgacgtgc gagacgtcca ctccttcgcg cgccagttgc
     1321 tcgcgcacga accgccccag gtgctcgtcg ccgacgcggg tcagcatcgc cggccgcagg
     1381 ccgagccgcg cgagcccgac ggcgaggttg gccgacgacc cgccgaggta tttcgcgaac
     1441 gactgcacgt cctcgagccg gccgcccacc tgctgaccgt acaggtcgac cgcggcgcgc
     1501 ccgagacaga cgacgtcgag cgcggccgct tcagccatcg cggtccctcg cgcctgcggg
     1561 ccaccagaac tgcgcgtcac tgcgcatcgt ccaggcgtgc tcgtcagtga attcgggcac
     1621 ggtgtaaggc cggtcaggca ggtgccgtat cacccacgag taatacatcc cgtagcccgg
     1681 cgcggcgcac tgcgcgtggt cgtgaccggc ggggatgcgg accgtgtcgt actgccgcac
     1741 cttgacgacg gcctcgccga gctccgcgtg gccgaacccc tgcggctcgg tgaagcggta
     1801 gtggtagatc tccggctgcg ggtggtggtg tggcgggtag ctcgaccagc cgccgggaag
     1861 cgtgacgacc tcgcccagca cgagttcgac ctcgtccggc gagttcttgc gatcgaagat
     1921 cgtgcgcacg aggcgcaggc agcgatcgcg cacctgtccc gcgccgcggt gctcgtccgg
     1981 cacgtcaccg ggcccgaaca cgcgcgcctc gaacacgcgc cggttgggac atcggtatac
     2041 cgtgagctcg gcgtcggtga ccgcatcgag cgtcacggac gtgccggtgc acacatggat
     2101 gcagctggca gattcgtcga acagcgagcg gcgctggaac tcgaagcgcc tgtcaccggc
     2161 cgtgccacgg acgtggccgt gcatcagcag ccaggccgtt tccacgtctg cgggctgctc
     2221 gagccgttcg ccggcgcgga gccgcaacac ggccacgccg agtccgaccg ggtcgtcctc
     2281 cgcgtcgtga cgtgtgatta ccgtctcgcc gtggccgaac ccggtgcggt ggtcggtcag
     2341 gtgcagcgtg ttggtggtca tgcgcccgcc cctccgtggg cttcgacgtc gatccaggcc
     2401 tgcgtggcgt gcgagcgctc gatcgcctcg atgaccgcct gcacgcgggc accctcgtgg
     2461 aaatccggcg tggcgccccg tgcgcgcgga tccacccgac gccgggcgat cgcggccagc
     2521 aggttacgcg attcgatgac cttgaggtcg ttgatgccga gcccgtggcc cggtgcaggg
     2581 cagaacgagc ggtagtcggc gtgctccggt cccgcgagga tcgtgcggaa cccacgcagg
     2641 cccgggcgat cgtcgttgcg atagagctgc agctcgttca tgcgttcctg gtcgaagcgc
     2701 aggctgccgc gcgtgccgaa caggtcgaac gcaagcccgc atttgtagcc ggtcgccacg
     2761 cggctgacgt cgaaactccc gcgcacgccg ttcgcaaact cgacgagcac ctgcgccgtg
     2821 tcttcgttct cgacctggcg cgcgcgtccc gtggttgctt cgcgccgttc gccgatcacg
     2881 gtcgacagcc gtgccatgac gcggccgagg tcgccgagca gcacgtgcgc gaggttgatc
     2941 aggtgcgtgc cgaggtcggc cagtgcgccc gacccggcga gcgcccgctc gcagcgccac
     3001 gtgaacggca ggccggggtc gctgaggtaa tcctcgaagt accggccgtg gaagccgatc
     3061 ggcgtgccga tctctcccgc gccgacgagc tctcgcgccg tctggagcag cgggttgcag
     3121 gcatagttga agccgaccag gtggcagagc cccgcgacat cggcggccga agccatctgg
     3181 cgggcgtcgg tcgcggacag cgccagcggc ttctcgcagg agacgtgctt gcccgccgcg
     3241 attgcggcca gcgccatgtc gcgatgcagg tgattgggcg tgcagacgac gacgacgtcg
     3301 acgtccggat cggcgaccag cgcacgccag tctccggtgc tgcggcgaaa gccgagttcg
     3361 cgcgcagccc gcgccgcacg gtcggggtcg gtgtctgcga ccagttcgag gaccggcggc
     3421 tcgacgtccg cgaacaccgt gccgaccgtg ctcaaggcga tcgcgtgcgc gcggccgatg
     3481 tagccggtgc cgatcaggcc gatgcgggcc gccgtcactg cagcgtcctc ggctcgaccc
     3541 ggacgggctg gcgcgtctcg tgcgaacgct gtgccgcatc ggccagttcg agcgcacgga
     3601 gcccgtcgag cgccgtcgca agctcgaccg actcgccgcc gcaggcgcgc acgaagtcga
     3661 cgagctcgcg ccggtaggcc tcggcatacc gctcgaggaa aaatggaagt gccggctcgg
     3721 tcgtgaagcc agcggcgctc gcgtgctcga cgtgcgtcgc ggtcgcgttg ttcgcgcgca
     3781 gcatgccggt cgccccgaaa gcctcgatgc gctggtcgta gccgaaactg cagcgacggc
     3841 tgttgctgat ctggcaaagc ctcccgctgg ccgtcacgag cagcacggcc gccgtgtcga
     3901 cgtcacccgc cgcggcgatc tcgggatcga cgaaacagcc accgcgtgcg tacacctcga
     3961 ccggttcctc ggcgagcagc cagcgcgcca tgtcgaagtc gtgaatcatc atgtcgcgaa
     4021 acagtccgcc cgagcgccgc acgtagtcga tcggcggcgg cgccggatcg cggctcgtga
     4081 tcgtcaacgt cgcgagcgga ccgatcgcgc cccccgccag ctcgcgcttc acgcgcgcga
     4141 acgacgggtc gtagcgccgg ttgaagccga gccgcagcac gatgccggcc cgttcggcgg
     4201 cctcgacgca gcgacgcgca cgcgccaggt cgaggtcgag cggcttctcg cagaacaccg
     4261 ccttgccggc ccgggctccc gcctcgatca ggtcggcgtg cgtatcggtc gggctcgcga
     4321 tgagcaacgc gtcgaccgcc gggtccgcga gcgcggcgtc cacgctcgcg accacggcgt
     4381 cacgttgcgc cgccagcgat tctgcggcgc cttcgtccgc gtcgcagacg caggacagcc
     4441 gcgccccggg tgtgcgggtg agattgtcgg catggatgcg accgatgcgt ccggcgccga
     4501 tcagtgcgac gcgaagcggg tcgcgcgaag ccgccacgtc agtggtcccg cggccggcgc
     4561 gtgccgagcc ggtacgcata actgatcgcg agcgtctgcg cgagacacag cgaggcggtg
     4621 agcgagcgga actgacgcac ctccgcgtcc ttgacctcga agcagagttc cgcctcgcgc
     4681 gcgatcgggc tcacgcggct gtcgctgatc gcgatgacgg gcgcgttgcg ctcgcgggcg
     4741 gccgccacga cttcggcagt ctcgcgagca tatggatgga agctgatcgc gatcatcagg
     4801 tcctcggctt caacgatgct cgcctgctcg gtgagcatgc cggccacgcc gtcgatgagg
     4861 tacgcccgct tctcgacgtg gcgcagcgcg tacgccaggt acgcggccac cggaaacgac
     4921 cggcgcatgc cgacgatgta gacagcgcgg gccgcctcga tcaggtcgag cgagcgttcg
     4981 aggtccgcgt cgcgcacgac ctccttcagg tgccgaagcg ccaggatgtt gttgtcggcg
     5041 aactcgcgca tcaggtcgtg cgcgccgcgc gtcgacgccg cgcccgagcc ctcgctgtac
     5101 tggcggatcc gttcggagta gttcgaactc ggggcgtgat tcaggatctc gtcccggaac
     5161 agcttctgga tgtcgctggc gccttcgtac ccgaacgtct ttgcgaaccg gacgatcgtg
     5221 gacggctgca ccttggagcg cgccgcgatc acggcgagcg tctgcagcgc cacgtcgttc
     5281 gggttctcga gcacgaactt cgcgacctgc ttcagtcgcg ggctcaggtt ctcgtatcgc
     5341 cgcacgattt cgttccgcag gctttcgggg ttcttcggag ctttcacgtc agggttcctg
     5401 gggcgcgccg cggcatgcta ggccagccgg aacgccaatt ccaccgccgg cacgggcatt
     5461 ccaccctggc cggcgatggc ttcagaactt cacctgcacg cccgccatcg cattccacga
     5521 gtagatctcg ttctccgcca gtcggcgacc ccgcccgctg gtgtagcgca gcggttcgtc
     5581 ggtcaggttc tgcatctgca ggtagacggt gaacatgtcc gtgaagtcgt agctcgcctt
     5641 gaagtcgagc tgcccgtggt cgtcgatgta gaggtcttcg tcggggcctt cgcccagcgt
     5701 atcgaggtac tcgctgcggt aggagtatcc gagtcgcagc cccacgcccg cgcgctcgta
     5761 gtacagcgcc aggttgccca cgtgctcgga ctgcaggaag aacgggatgt cgtcgtcccg
     5821 gtcgaacact tcggcgctgc tgtccgagta cgtgtaaccg agcgagaatc cgagcccgtc
     5881 gaacggtgcc ggcaggccgg tgaacgactg ctggtagttg aactcgatgc cgaagatgtc
     5941 cccggactcg gcgttctccg gtcgcagcac ctcgagttcc tggtagaagc ggccctcgaa
     6001 ctcctcctcc tcgagcacct ggaaccgcgt gaagaccggg ttctcgaggt ccttgaagaa
     6061 cacgccggtc gcgatgacgc ccgccggcag gtaccactcg accgagacgt cccagttcat
     6121 cgattcgatc ggctcgaggt cggagttgcc ggtttcgagc gcgccctcga agcacgggtc
     6181 cggtgcgttc gagcagtcct ccgggtcgag ttcctcgatt tcgaaactgc gaaatggcac
     6241 gagctgctcg tatccagcac gcccgatcgt gttggtccag gcggcacgaa cgacgagctc
     6301 ctgtcgtggc gcccagcgca tctgcacgcc gggcagccag tttgtgtagt ccttggagcc
     6361 ggtcactttc ggcggcaccg ggtcgatgtc gccgtcgacg aactcgaggt cgtacccctg
     6421 gtagtcggct tcggtcttct cgacacggac gccaccggtc aacgacagcg tgggcgtcac
     6481 gtcgagctgc agcatcaggt agccggcgga gatcttctcg tcgatcacgt aatcgacgcc
     6541 gtactcctcg gcgaccgtgt ccccgtcgtt gacctcgaac gcagcctggt tgtcgcggaa
     6601 gaaacgctgc gccgcctcga agttgatctt cggtccgaac gtatagcaac gctcgctgca
     6661 gtagaaatcg gggtcgccgc tgcgggcgac ttgcgagagc aggaaatcgt cgtcgctgtc
     6721 gaagccgtcg tagacggtgg cgctctggtc gctggtcttc tcacggtcga gcagcttggc
     6781 gccgaacttc acgaagccgc tgcggccgcc gaagctgagg tcgcgacgca ggtcgagctg
     6841 cgcgatgttg aggtcttcct cgaccatctg cttcgcacgg aagtgctcgt cgaactcgta
     6901 accttccgtg tcctggaact cgggaccggc ggtcacgtcg aagaactccc ccgaggtgtc
     6961 gtaggtcatc ggcagggcgt cgtccagctc gaacacccag ccgtagtccc acggggtgtc
     7021 ctgctcggac ttgccgagcg tgtacgaggc accgagcgtc cagccactgc cgaaaccgaa
     7081 gtcgccgccg agcgtcgagc tctggatcga ctggatctcg cggcggctct gcaactcgcg
     7141 cgagccttcg ccctcggtga aggtcccgga ggtcgcggtc tggtcctcga gatcgccgtt
     7201 gcggtagtcg tagatcgttt caggctgcag ttcgatgtcc tcgaaccggt tgtagaggtt
     7261 gcggaaatag agcttggtcg tgtcgctcgg tcggaactcg aggttcgcca cgacccccga
     7321 acgcttgcgc tcgatcacgt aatcgcgcag caccagctcg tccgggacga agaagtcgcc
     7381 ctcctcctgc cacgggtcgc cgccctgcag gttgtgcgag gcgtactccc gctcggagta
     7441 gctgcccgag agcacgatgc cccagcggtc ctcgccgaac gtctggcccc acgcgagcgc
     7501 cccgccaaat gggttttcgc cgttcaggtc gtagtagccg tactccatgg acgcgctggc
     7561 gaagtgaccg tcttcctggt cgaaggcgct cggcgtgacg atgttgatcg agccaccgat
     7621 cgcgttgccg tccatgtccg gggtgaccga cttcacgacc tcgagcttgg ccagcaggtt
     7681 cgcgggcacc gtgtcgagcg cgatgcgccg atcgtccggc tcgggcgtgc cgaggatctg
     7741 gttgtcgagc gtgacgttgt tgagcccggg gtcgatgccg cgcaccgtca cgtagcggcc
     7801 ctcgccctgg tcggtcgtcg tcgacacgcc gggcagccgc tgcagtgcct cggccgcgtt
     7861 ctggtcgggc agcttgcccg cgtcgtccgc cgtgatcgaa tccttgatca ccgctgacga
     7921 cttcttgtcc tgcagcgccg tggccagcgc gaggcggaaa cccgtcaccg tgatttcctc
     7981 gacgcccgcg ccgagcaacg cgaatgtctg cgtcgcggcg cccgtcgccg ggacctcgag
     8041 cgtctgcgtg gcctcgcgga atccgacgga ggtcaccgtg agctggtagc tgcccgccgg
     8101 cacgtccgcg acgcggaact caccgctgcg gtccgcggtg acggtgcggc ccagcggctc
     8161 gatccggacg gtcgaattcg gcaacggccg tcccgtggac gaatcggtca cgcggcctgt
     8221 cacctctccc gccactccga cggcaacggg caacagggcg agggttaaag cgcctgcgac
     8281 gacgttctgc ttggctctca tgacgttgtg tcctggttcg tggtggttcg gcccgcacga
     8341 cggcaacagc gggtcgtgca atcccaacag cggttgatgg ctgtcccgtg acaccgatgg
     8401 aatcgtcgtt ccattctgtc tcgatcatag aacaactgtt ctgcgaatgg caacggcggg
     8461 ccgcgggcag ccaggacgct gcccgggcgc tcgggggtaa ggcggaggcg ccgggtcgtt
     8521 tacttcatcg tcggcatgac gaactgcgcg ccctcgcggc gcgaggtcgg ccagcgcgac
     8581 gtgacggtct tgagacgcgt gtagaaccgt acgccgtcgg gcccgtgcac gtgcagtgcg
     8641 ccgaacagcg agcgcttcca gccgccgaac gagtggaagg ccatcggcac cgggatcggc
     8701 acgttgatgc cgaccatgcc ggcctgcact ccttgcgtga acgtgcgcgc ggcctcgccg
     8761 ctcgcggtga agatcgccgt gccgttgccg aattcgtgcc cgttgacgag ctcgagcgcc
     8821 gcgccgaggt cgggcacgcg cacgacgcag agcacggggc cgaagatctc ctcgcggtag
     8881 atgcgcatcc gcggctcgac cgcgtcgaac aggcaggggc cgaggaaata cccgccctcg
     8941 tgtcccttga tccgcaaact gcggccgtcg accacgaggt tcgccccttc ctgcacgccg
     9001 agctccacgt agccgcgcac ccgctcgagg tgctcgcgcg tgacgagcgg ccccatctcg
     9061 accccgtcgg ccaggccgga gccgaccttg agctgtgcga gctgtgcgcg cacggcctcg
     9121 atcagcggct cggccgcgtc gccgacggcc accgcgaccg aaatggccat gcaacgctcg
     9181 ccggccgagc cgtacgccgc gccgatcagc gcgctcgctg cgttggccgt gtcggcgtcc
     9241 ggcatcacca ccatgtggtt cttggcgccg cccaaggctt ggacgcgctt gccgtgcgcc
     9301 gaggccgtcc ggtagatgta ctcggccacc ggcgtcgagc cgacgaaact gaccgcgccg
     9361 acgcgcggat cggtgagcag cgtgtcgacc gcttcgcggt cgccgttgac gacgttgaag
     9421 actccgtccg gcaggccggc ttccttgagc aactgcgcga gccgcatcgg acagctcggg
     9481 tccttctccg acggcttgag cacgaacgtg ttgccgcacg cgatcgcgac cgggaacatc
     9541 cacatcggca ccatcgccgg aaagttgaac ggcgtgatgc ccgcacagac gccgaccggc
     9601 tggcgctgcg accagctgtc gacgccggtg ccgacgctct cgctgaactc gcccttgagc
     9661 atctgcggga tgccgcaggc gaactcgacg acttcgatgc cgcggatcac ttcgccacgg
     9721 gcgtccgaga acaccttgcc gtgctcggac gtgatcagcc gcgcgagttc gtcgaggtgc
     9781 gattcgagca gcgccttgta gcggaacagc acgcgcgcgc gctgcagcgg cggcgtcgcc
     9841 gaccacgccg gcagcgcggc ggccgccgcg gcgatcgcgg cctgcgtttc ggcttcactc
     9901 gcgaaggcga cgtggccccg gagcgcaccg gtcgacggat cgtgcactgc gccgcgacga
     9961 ccggaggtgc ccgcggcttc gcgtccctcg acgaagtgac cgagttcgaa cgtgtcgggt
    10021 gtcgggacgg cggtcggtcg cggagcgttc atgtggcgag tccgtcgtgg agcgaggcgg
    10081 ggcgggcatt gtaggcaaaa atggggacac tcccgatttt ctcccacggc gtcgcttccg
    10141 ccggggcagc gacggcgcgg aaaccagccg tgtccctgtt cgacgggaca gtcggagcgt
    10201 cccgccggcg tcagtacacg cggtcgcctt ccagcagtgc gtccgccggc agcgtttcct
    10261 gctgcgcaac ctcggtgtag agcggcgtga aatcgggcgc ggtctcgcga aacagctggt
    10321 cgaacccgtc gatcacgaag tacgtgggct ggaaggtgtc gatcttgtac ttcgtgcgca
    10381 tcacgcggcg caggtcgaag ccgatgcgcc gcggctgcgg gctgtcgagc gcgtggacga
    10441 tctcgctcgg cgacgacagg atgccggcgc cgtagatccg caatccgtcg cgcccgcgga
    10501 tcagaccgaa ctcgaccgtg taccagtaga ggcgcgcgag gtactcgagc gcgtgcaggc
    10561 cttcggcctt ctgcccgccc cgtccgtacg cctgcatgta gtcggcgaac acgggatcga
    10621 acagcagtgg cacgtggccg aagaagtcgt ggaacacgtc gggctcgacg atgtagtcga
    10681 attcggcttc ctcgcgcagc cacaccgtca ccgggaaccg gcgcgccgcg aggtgcccga
    10741 agaacacggg ctccggcagc aggcccggta ccgcgacgac ctcccagccg gtcgcacgcc
    10801 cgagccgctc gttcacctgc tcgaatcgcg ggatgcccgc gccgtagtcg agcgtctcga
    10861 tgacgcgcag gaactcgtcg cacgcgcgct gcggcacgag cttgatctgc cgctggtaga
    10921 gccggcgcca tcgctgctgc gctgcgtcat cgtaggccga atagtcctgc gtgacggtgt
    10981 agtcgggccg catgtcgcgg tagtcgccgc gcaggccgtg actgctcgct ccgtacgtcg
    11041 cagggggtgc cgacatgcct ggatccatga tgggtctgaa ggtgcgtcct tttacgtccg
    11101 gcgggccggt ttgccaagcg gccgggttcg ggccgggccc agaggctgcg gtatagtggc
    11161 gcttccgcgc cggccgcgcc gccgcgaccc atcccgcagc cgaggattga actccatgaa
    11221 gctttggacc gctcttgcga tcgctccgtt catgatggcc accggtgccc tggccgaggc
    11281 cgcatgcgtc tatccgcagc cgccgcaggc cctgccgaac ggcggcagcg caaccaagga
    11341 agagatgctg gccgcgcaag gcctcgtcaa ggaatacgtc aataacgtgc agggaaccta
    11401 cctgccgtgc ctcgagaagg agcgcgacga agcgaccggc gcgctcgaca acatggaccc
    11461 ggagtacacg gccaagaagg gctcgatcga ggcaatccac gcgaagaagc acaacgctgc
    11521 gctggacgag ctgcaggcct tcgtcgatcg ctggaacgcc gagaagaagg cctttaccgc
    11581 gaagtcggac aagtaagcgg gcggccgaca tcgaccccgc gaccgtcacc gccgccgccg
    11641 cgctgccctc gcctgccgaa gcggaggcgc tgatccgagc gcgcgtgcag ccgctgccgg
    11701 tggaatcacg cccgctcgcc tcgctcgccg gggcggtgct ggcccaggcg gtgcgctcgg
    11761 agcgcgacca gccgccgttc gatcgcgtga tgatggacgg catcgcgctc gcctcgcagg
    11821 cgtggcgcgc cggccgtcgc cgctaccgca tcgccggcac ccaggccgcc ggcctgccgc
    11881 cgcggcggct cgaagatccg gagacctgcc tcgaggcgat gacgggcgcc gtgctgccgc
    11941 ccgggtgcga ctgcgtcgtg ccggtcgagc acatcacggt cgaagacggc gtcgcggtcg
    12001 tcgacgaggt cacgcccgtc gaaccgttct ggaacgtgca cacgcgcggc ctcgactgtc
    12061 gcgagggcga catgctgctg acccgcggca ctgtgctcgg cgcgcccgaa ctggcggtgc
    12121 tcgcgtccgc cgggctgccg cgcgccgacg tgcacgcgga tccgcgcatc gtcatcgtcg
    12181 ccaccggcga cgagctcgtg gcgcccgacg agccgatcga agcctggcag gtgcgtcgct
    12241 ccaacagtta tgcgctgcgc ggtgccctgc acctgcgcgg ctttcatcgc gtcgccgagg
    12301 atcacctgcc cgacgaccac gccgtgctgg cgcagcggct cgcgacgcac ctggccacgc
    12361 acgacgtcgt gctgctgacc ggcggcgtct ccatgggccg gttcgaccac gtgccgagcg
    12421 tgctgcgcga actcggcgtc gccgaagtct tccacaaggt cgcgcagcgg ccgggcaagc
    12481 cgatctggtt cggcatcggc ccggccggcc agacggtctt cggcctgccc gggaacccgg
    12541 tctcgtcgct cgtgtgcctg ttgcggtatg cggtgccggc gatctacgcg atgctcggcg
    12601 cgaagccgcg cccgcccgag caggtgccgc tcggcgccgg gcacctcgtc aaggccgggc
    12661 tctggcacta cctgcccgtg cgcgtcgagc aaagccccgc gctcggcgtc gtggcgatgc
    12721 cgcggccgac gcgcggctcg ggcgacttcg tgtcgctgct gggcacggac ggcttcgtcg
    12781 agctgccgcc ggggccccgc gaaatgcctg cgggctgcgt cgttgcgttt taccgatggt
    12841 gacgcaccgg tccgcgatgg ggacaatgca ccgatgacca gcgttaccac cgtaccgatc
    12901 gccctgcacg accgcctcgg ccgcccgatc cacgacctgc ggatctcggt catggaccgg
    12961 tgcaatttcc gttgcccgta ctgcatgccg caggcgacgt tccacgagaa ataccggttc
    13021 ctgaagtcgg ccgaacgcct gacgttcgag gagatcgcgc ggctcgcgcg gctggctgcc
    13081 gggctcgggg tccgcaagct gcggctgacc ggcggcgagc cgctgctgcg cactggcctg
    13141 cccgagctgg tcgcggagct gtcgaccatc gaaggagtcg aggacctggc gctgacgacc
    13201 aacggcgtgc tgctcgccca gcacgcggcc gagctcaagg gcaacgggct gcaccgcgtg
    13261 accgtcagcc tcgattcgct cgatgatgcc gtgttcacgg ccatgtcggg cggctacggc
    13321 ggcctggctc aggtgctgca gggcatcgac gcggcgctcg aatccggcct caagccggtc
    13381 aaggtgaaca ccgtcgtgca gcgtggccgc aacgaccaca cggtgctcga cgtgctcgag
    13441 ttcttccgtg gcaaggacgt gacggtgcgg ctgatcgaat acatggacgt cggcacgcgc
    13501 aatcactggg atccgggcga cgtcgtgccg tcggccgagc tgctcgaccg gatccgcgcg
    13561 cgctggagcg tgtcgcccgc accgggccgc tatcgtggcg aggtcgcgga acgctacgtc
    13621 tacgacgacg gcgccggcga gatcggcttc gtgtcgtcgg tgagcgcccc gttctgcggc
    13681 gactgcaacc gcgcacgcct ctcgtcggaa ggtgtgctct acacgtgcct gttcgcgtcg
    13741 cagggcgtcg acctgcgctc gccgctccgt gcaggcgcca ccgacgtcga gctgctcgac
    13801 ctgctgcgcc gcgcgtggtc gcagcgcgcc gaccgctaca gcgaactgcg cggcgaactg
    13861 cgcacgcgcg aggcgccgct gcgcaaggtc gagatgaact acatcggcgg ctgaaggcga
    13921 tgagcgcact cacgcacgtc gactcgagcg gcaggccgac gatggtggac gtcggcgcca
    13981 aggccgtgac gcaccggacc gccgtcgcgg aaacgcgcgt gcggtttcca gccgccgtgg
    14041 cggctgcgtt gcgcgatgcg cagttcgcga cggccaaggg ccctgtgttc cacaccgcga
    14101 tcgtcgccgg cgtgatggcc gccaagcgca cgcacgagct gatcccattc tgccacccgc
    14161 tcggactcga gcgctgcgac gtggcgatcg acatgcaggg cgacgaagcc gtgatccgct
    14221 gcaccgtgtc gatccaccac aagaccggcg tcgaaatgga ggcgctcaca ggggcctcgg
    14281 tcgcggcgct caccgtgtac gacatgtgca aggcgctgtc gcacgcgatc gtcattgccg
    14341 agacgaggct cgtggagaag cacggcggca agcgcgacgt gacggcggcc tgatgacgcg
    14401 ccggcgaaac cccggaggct gacgaattgg cgaacaggat tcacctgcag tatttcgcgg
    14461 tgctgcgcga gcaggccggc cgcagcgagg aaacgctcga gaccgaggcg ctgacgcctg
    14521 ccgaactcta cgagcaggtc cgtgcgagcc acggcttctc gctgccgcgc gcgatgctgc
    14581 gcgtcgccgt caacgacgag ttccgtgact ggaccgtacc gctcgcgagc ggcgaccgcg
    14641 tcgtgttcat cccgccggtg gcggggggct gatgcagcct ttctcgttca gcgattcccc
    14701 gatcgacgcg gccgccggcc gcgccgcgct cgcggatccg gcctgcggag gctacgcgag
    14761 cttcgagggc tgggtgcgcg accacaacga aggtcgccag gtccgccacc tcgagtacga
    14821 ggcgttcgcg acgctggccg tgcgcgaggg tgagcgcatc gtcgcagaag cgatcgagaa
    14881 gtacggcgtg agccgcgccg cgtgcgtgca ccgggtcggc gacctcgcgc tcggcgagat
    14941 ggccgtctgg gtcggcgtga gctcgccgca tcgcggcgag gcgttcgccg cctgccgcta
    15001 catcatcgac gaagtgaagc atcgcgtgcc gatctggaag aaggagcact acgtcgacgg
    15061 cgactcgggc tgggtcaact gcgagcgctg cgcacagccc tcggcacatg agcacgagca
    15121 cgggcaggcg caccagcccg accacgcgca cggtcacgaa cacgtgcatg cgacggggtc
    15181 tcgccccgac acgcgcgttc cccacggtgt cccggactac tcgcgccaga tggcgctgcc
    15241 tgaagtcggt cgcgacggcc aggcccggct gcggcagggc tcggtgctgg tgattggtgc
    15301 cggtggactc ggcgtgcccg tactgcagta cctggccgcg gccggcgtcg ggcggatcgg
    15361 catcgtggat ggcgacgtcg tcgaggcgag caacctgcat cgccagccgc tgtacggctg
    15421 ggccgacctc ggcgcgaaga aagcacgcgt cgcggccgag cgactgcgcg cgctcaaccc
    15481 cgacgtccgc gtggagacgt acgacgagcg cgcgacgccg gccaacatct ccgactggct
    15541 cgcgcgctac gacctggtgg tcgactgcac cgacaacttc gcgaccaagt tcctcgtcaa
    15601 cgacgccgcc gtgagcctcg gcaagccggc ggtcttcgcg agcatctacc agtacgaggg
    15661 ccagttgcag acgtacctgc cgcgccccga ctggccctgc ctgcgctgcc tgtggccgga
    15721 ggcgccgcgc gacggcctgg tcggcaactg cgcagaagcg ggcgtgctcg gggccgtgcc
    15781 ggccgcgctc ggcgcgatgc aggcgatgca agcgctcaag ctgctgctcg ggctgcccgc
    15841 ggacgcgcgc cctgccctgc acacgttcga cctgctcggc atgcactggc acacgctcaa
    15901 ggccgcgcgc aaccccgcgt gcgaccacga cacgcgtgtc ctgcaggctg ccgacgccga
    15961 agacgacgaa ctcgaacgcg aatacccgga cctgcgcgcc gcgtcggcgt ccggcctcac
    16021 gctcgtggac atccgggaac cctgggaacg cgcgagcgac gatccggcgc ggcagatccc
    16081 gatgcacgtg cctctgagcg cgttcgtcaa tggcgaagcc gcgctgcccg agggtcgtta
    16141 cctgctggtc tgcgcccacg gcgtgcgcag ccacgcgctc gccgagcacc tgcgttcgca
    16201 cggccaccgc gaggtgtatt cgctggccgg tgggctggca gggctcgccg gggtttaggg
    16261 cagcagctcg tacatcgcct cgccccccgg caggtgctgc ctgaactgcc agccggctga
    16321 ttcgtagaag cgctgcgccc tcgtgcccgg ttcagtgctc agccacaaac gcctgcagcc
    16381 ctggtcgaag agccaggcga ccatctcgtc gtggagtcga cgaccgacgc cacgcccttc
    16441 ggcagccggc gtgacgaaca acgcccagat gttgccgttt tccctgttgc cgacggcaaa
    16501 gccgagcacc tgaccgtcct cgacggccac ccagccccgg ccgcgcgtct ggatttccgg
    16561 tacgtaactc gccgcggtaa tcgcggccga agccagccgg ttctcgatga ccgactggcg
    16621 gacacggtgc atgtccggaa tgtcctcgac cgtggcgact cgcacgacga cggccatgtc
    16681 gatttctcgg ctgagcgctt gttcagggcc gggtctgccc gctgccgcgc accacgtact
    16741 tgtag
//
LOCUS       DASPFR010000196         8569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1521654, whole genome shotgun sequence.
ACCESSION   DASPFR010000196 DASPFR010000000
VERSION     DASPFR010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8569
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521654"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VD737_04990"
     CDS             complement(<1..255)
                     /locus_tag="VD737_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09949.1"
                     /translation="MPRCKDLVLVLVAGGCVVAGLALPSLTHAQGADATPLGFYAGGG
                     ASYSNVSVERPGSCYGDCYSWWGEYPTYDEGDGDYSFVGHV"
     gene            complement(370..1587)
                     /gene="dapC"
                     /locus_tag="VD737_04995"
     CDS             complement(370..1587)
                     /gene="dapC"
                     /locus_tag="VD737_04995"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HXW09950.1"
                     /translation="MNPRLDLLQPYPFERLRALLAGARPPAALRHIPLSIGEPKHAPP
                     PFIAQALVEGLRTLGNYPVTLGLPRLRETISRWLERRFGLPAGAVDAETMVLPANGTR
                     EALFAFTQAAVDADGSRGAPSLVAMPNPFYQIYEGATLLAGAQPVFLNCTAATGYMPD
                     LDAVDDQTWNRCQVLFLCTPGNPTGAVMPETLLRRALELADRFEFVVASDECYSELYF
                     DENAPPAGLLQAAWNAGHRSFERCVVFHSLSKRSSVPGLRSGFVAGDPRLLAKFRLYR
                     TYHGCAMPEHVQLASIPAWQDEDHVVANRRLYREKFRRVAPMLSEALQQDVPIPAGSF
                     YLWPAVGDDERFARELFEQQHVTVLPGSYLARDTDAGNPGRGRVRISLVASVDDCVTA
                     AERVATYGCERRR"
     gene            complement(1640..4360)
                     /gene="glnD"
                     /locus_tag="VD737_05000"
     CDS             complement(1640..4360)
                     /gene="glnD"
                     /locus_tag="VD737_05000"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HXW09951.1"
                     /translation="MNSPVAYEELDSGPAPTAPQPPWSFLATLPQALRGSEPAIAAFR
                     QALADGDAALRQRFEHDEPVEMLVRDRARMVDVVLRSAWELHVGAGARDVDLIAVGGY
                     GRGELHPCSDIDVLILLPKSEVIDAETGIERFLMFLWDIGLEVGHSVRTIDDCQRESA
                     ADVSVATTFIEARLVAGPEHLFQSMRRALGQDQVWSSKAFFEAKVAEQQTRHHRYHDT
                     GYNLEPNVKASPGGLRDIQTIGWVAKRHFGAETLDELVGHGFLTGAELRKLRQAQAFL
                     WKVRFALHVLNGRREDRLLFDHQIRIAKMFGYEDATYTLAVEQFMQRYYRTVMDVSLL
                     NEMLLQLFREAILSGEGESAIVPLNPRFQLRNDYLEVTSDDTFVRNPSAILELFRVMQ
                     DTPGIRGVRATTIRLLGRHLWLIDEEFRQNPRHHRLFLELLRAPVGVTHELRRMNLYG
                     VLGRYIPAFGRVVGRMQYDLFHAYTVDAHILFVLRNLRRLALPRFEHELPEMSRLMAA
                     IQRPEVAYLAALFHDIAKGRGGDHSELGAVDAEAFCLEQGLSRYDARLVAWLVRHHLI
                     FSVTAQKKDISDPKVIQEFARTVGDQTHLDYLYLLTVADVRGTNPKLWNSWKSSLFHD
                     FYERVRRALRRGLETPLEHEELVAETRAHALELLDLEHVSRARAAQIWSRFNEMYFLR
                     YSPAEIAWQTTLLAATEPDDVSLLVAVRTEVERGGNAVLTVGPHRQQNFARTTALLDQ
                     MGLNIVDARITPLDGGLGLDTYLVLEDTGQPIADRQRAQQIELQLRRVLQNPESGPPV
                     VTRRAPRQVRMFSTPTQITFSEDPLQNRTVLEIIAGDRPGLLSEIGKVFLAEQIDVIT
                     AKIMTIGERAEDVFYVTDAQGRPLGEPARKRLQESLTAALDRRDAENRLA"
     gene            complement(4357..5178)
                     /gene="map"
                     /locus_tag="VD737_05005"
     CDS             complement(4357..5178)
                     /gene="map"
                     /locus_tag="VD737_05005"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HXW09952.1"
                     /translation="MQHSPTPTTAAAAEASGRVLLKGPAEQAAMREAGRLAAEVLDMI
                     GQHVVPGVTTEELDRLCHEHIVQVQQAVPAPLGYRGFPKSICTSVNHVVCHGIPGERK
                     LKQGDIVNVDVTVIKDGFHGDTSRMFHVGKPLPHAVRLVDLTYQAMWLGIREVRPGAR
                     LGDIGATIQRFVESNHYSVVREYCGHGIGRGFHEDPQVLHYGTAGTGLMLEPGMTFTI
                     EPMVNAGRRDVRLLPDGWTVITRDHSLSAQWEHTVLVTEDGFEVLTLGAGEQLPA"
     gene            5452..6129
                     /gene="rpsB"
                     /locus_tag="VD737_05010"
                     /pseudo
     CDS             5452..6129
                     /gene="rpsB"
                     /locus_tag="VD737_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
     gene            6550..7440
                     /gene="tsf"
                     /locus_tag="VD737_05015"
     CDS             6550..7440
                     /gene="tsf"
                     /locus_tag="VD737_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003650628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HXW09953.1"
                     /translation="MAVTAEAVKALRERTGAGMMECKKALVETNGDLEAAAEIMRKSG
                     LAKADKKAGRVAAEGVIVVERSADGRQAAVVEINSETDFVARQDDFQSFAGEVARAAL
                     TQRPASLEALLDTRLASGKTVEETRRELIARIGENIGVRRFGLVEAETPLHTYRHGTR
                     IGVVVAMQGGDETLGHDLAMHIAAINPQYLSADEVPAESVAKEKEIFVSQVAQDPKTA
                     GKPAEIVAKMVDGKVRKYLGEITLLGQPFVKDDKQSVEQVLKKANARVVRFVRYEVGA
                     GIEKKQENFAAEVMAQVRGA"
     gene            7525..8256
                     /gene="pyrH"
                     /locus_tag="VD737_05020"
     CDS             7525..8256
                     /gene="pyrH"
                     /locus_tag="VD737_05020"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HXW09954.1"
                     /translation="MEPTADPQPRNRRILVKLSGEALMGDADYGIDPAFLRRLANEVR
                     SVRELGVEIALVLGGGNLFRGAGLARAGMDRVTGDHMGMLATVMNALAMQDALESMGV
                     ATRVMSAVRINQVCEDYIRRRAIRHLEKGRVTIFAAGTGNPFFTTDTAASLRAIEIEA
                     DLLLKATKVDGIYDADPVGNPNARRYDRLTYDQVLDQRLNVMDATSIVLCRDHRLPLR
                     VFNLNDPGALVRAASGADVGTLVTI"
     gene            8290..>8569
                     /locus_tag="VD737_05025"
     CDS             8290..>8569
                     /locus_tag="VD737_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HXW09955.1"
                     /translation="MLEDIKKDANDRMGKCVAAFRQELKRLRTGRASTSLVDHIRVDY
                     YGAESPLNQVANVAVEDARTITIVPFDKQMVSTIEKAVMKSDLGVTPTT"
BASE COUNT         1413 a         2848 c         3027 g         1281 t
ORIGIN      
        1 cacgtggccc acgaaggagt agtcgccgtc gccttcgtcg tacgtggggt attcgcccca
       61 ccaggagtag cagtcgccat aacaactgcc cgggcgttcg acgctgacgt tcgagtagct
      121 ggcgccgccg ccggcataga agccgagcgg cgtggcatcg gcgccctggg cgtgggtgag
      181 cgatggcaga gcaagcccgg cgacgacgca gccgccggcc accagcacga ggacgagatc
      241 cttgcagcgc ggcatggcga gcctccaagg acgattcgat cgtctctttt ctaggcccgc
      301 ggacgagctc cagcaatcgt ggctagttgg tgacgcgccg gcgtgcggcc cgagactgag
      361 tctcgccttt cagcgtcgtc tttcgcaccc gtaggtggcg acgcgttcgg cggccgtcac
      421 gcagtcgtcg acgctcgcga ccagcgaaat gcgcacgcgt ccgcggcccg ggttgcccgc
      481 gtcggtgtcg cgagcgaggt aggagcccgg cagtacggtc acgtgctgct gctcgaaaag
      541 ctcgcgggcg aaccgctcgt cgtcgccgac cgcgggccac aggtagaagc tgccggcggg
      601 gatcggcacg tcctgctgca gcgcctccga gagcatcggt gcgacgcgac ggaatttctc
      661 gcggtacagc cggcggttcg cgacgacgtg gtcttcgtcc tgccacgccg ggatgctcgc
      721 gagctgcacg tgctccggca tcgcgcagcc gtgataggtg cgataaagcc ggaacttcgc
      781 cagcagacgc ggatcgccgg cgacgaaccc cgaccgcagg cccggtacgc tcgagcgctt
      841 cgagaggctg tggaacacga cgcatcgctc gaagctgcga tgaccagcgt tccacgcggc
      901 ctgcagcagg cctgcgggcg gtgcgttctc gtcgaagtag agttccgagt aacactcgtc
      961 cgacgcgacc acgaactcga accggtcggc cagttcgagc gcgcggcgca gcaacgtttc
     1021 gggcatcacg gccccggtcg gattgcccgg cgtgcagaga aacagcacct ggcatcggtt
     1081 ccaggtctgg tcgtccactg cgtcgaggtc gggcatgtag ccggtcgcgg ccgtgcagtt
     1141 caggaacacg ggctgcgcgc cggccagcaa cgtggccccc tcgtagatct ggtagaacgg
     1201 gttcggcatc gccaccagtg acggcgctcc acggctgccg tccgcgtcga ccgccgcctg
     1261 cgtgaacgcg aacagtgcct cgcgtgttcc gttcgccggc agcaccatcg tctccgcatc
     1321 gacggcgccg gcgggcaacc cgaatcgccg ctcgagccag cgcgagatcg tctcgcgcag
     1381 ccgcggcagg cccagcgtca cgggatagtt gcccagcgtg cgcaggccct cgaccagcgc
     1441 ctgcgcgatg aacggcggcg gagcgtgctt cggctccccg atcgagagcg ggatgtggcg
     1501 cagcgccgcc ggcggtcgcg cgcccgcgag cagcgcgcgc agccgctcga acgggtaggg
     1561 ctgcagcagg tccaggcgcg gattcaccta ggaaaagtgt ctagcggtta gcggttagta
     1621 agaccaggaa ggctcggtgc tatgcgaggc gattttccgc gtcgcggcgg tcgagcgcgg
     1681 cggtcaggct ttcctgcagc cgcttgcggg ctggctcgcc gagcgggcgt ccctgggcgt
     1741 cggtgacgta gaagacgtcc tcggcgcgct cgccgatggt catgatcttg gcggtgatca
     1801 cgtcgatctg ctccgccagg aacaccttgc cgatctccga gagcaggccg ggccggtcgc
     1861 ccgcgatgat ctcgagcacg gtgcggttct gcagcgggtc ttcactgaac gtgatctgcg
     1921 tcggcgtcga gaacatgcgc acctgccgcg gcgcgcgacg cgtcacgacg ggcggcccgg
     1981 attcggggtt ctgcagcacc cgtcgcagct gcagctcgat ctgctgggcg cgctgccggt
     2041 cggcgatcgg ctgcccggtg tcctcgagca ccaggtacgt gtcgagcccg aggccgccgt
     2101 cgagcggagt gatgcgcgca tcgacgatgt tgaggcccat ctggtcgagc agcgcggtgg
     2161 tgcgcgcgaa gttctgctgc cggtgcggcc cgacggtcag cacggcgttg cccccgcgct
     2221 cgacctcggt gcgcacggcc acgagcagcg acacgtcatc gggttccgtc gccgcgagca
     2281 gcgtggtctg ccacgcgatc tcggccggcg agtaacgcag gaagtacatc tcgttgaagc
     2341 gtgaccagat ctgcgctgcg cgggcgcgcg acacgtgctc gagatcgagc agttccagcg
     2401 cgtgcgccct ggtctcggcc acgagttctt cgtgctcgag cggtgtctcg aggccgcgcc
     2461 gcagcgcacg tcgcacgcgc tcgtagaaat cgtggaacag cgacgacttc caggaattcc
     2521 agagcttggg gttggtgccg cggacgtcgg ccaccgtgag caggtacagg tagtcgaggt
     2581 gcgtctggtc gccgaccgtg cgggcaaact cctggatcac cttggggtcg gagatgtcct
     2641 tcttctgcgc cgtcaccgaa aagatcaggt ggtgacgcac gagccacgcc acgaggcgcg
     2701 cgtcgtagcg cgagaggccc tgttcgaggc agaacgcctc ggcgtcgacc gcgcccagtt
     2761 cgctgtggtc gccgccgcgg cccttggcga tgtcgtggaa cagcgcggcg aggtaggcca
     2821 cctcggggcg ctggatcgcc gccatcagtc gcgacatctc cggcagctcg tgctcgaatc
     2881 ggggcagtgc gaggcggcgc aggttgcgca gcacgaacag gatgtgcgcg tcgaccgtgt
     2941 aggcgtggaa caggtcgtac tgcatgcggc cgaccacgcg accgaacgcg gggatgtagc
     3001 ggccgagcac gccgtagagg ttcatgcgtc gcagctcgtg cgtcacgccc accggcgcgc
     3061 ggagcagctc gaggaagagc cgatggtggc gcgggttctg cctgaactct tcgtcgatca
     3121 gccagaggtg gcggccgagc agccggatcg tcgtcgcgcg cacgccgcgg atgccgggcg
     3181 tgtcctgcat cacgcggaac agctcgagga tcgccgaggg gttgcgcacg aaggtgtcgt
     3241 cggacgtcac ttccaggtag tcgttgcgca gctggaagcg cgggttcagc ggcacgatcg
     3301 cgctttcgcc ctcgccggac aggattgcct cgcggaagag ctgcagcagc atctcgttga
     3361 gcaggctcac gtccatgacg gtgcggtagt accgctgcat gaactgctcg accgcgagcg
     3421 tgtacgtcgc gtcctcgtag ccgaacatct ttgcgatgcg gatctggtgg tcgaacagca
     3481 ggcggtcctc gcgccggccg ttcagcacgt gcagcgcgaa gcgcaccttc cacaggaacg
     3541 cctgtgcctg gcgcagcttg cgcagctccg ccccggtcag gaagccgtgc ccgacgagct
     3601 cgtcgagcgt ctcggcgccg aagtggcgct tggcgaccca gccgatcgtc tggatgtcgc
     3661 gcagcccgcc cgggctcgcc ttgacgttgg gctcgaggtt gtagccggtg tcgtggtaac
     3721 ggtggtggcg tgtctgctgc tccgccacct tggcctcgaa gaaagccttc gatgaccaga
     3781 cctggtcctg cccgagcgcg cggcgcatcg actggaacag gtgctcaggg ccggccacca
     3841 gccgggcctc gatgaaggtg gtcgcgacgc tcacgtcggc ggcgctctcg cgctggcaat
     3901 cgtcgatcgt gcgcaccgaa tggccgactt cgaggccgat gtcccacagg aacatcagga
     3961 agcgctcgat gcccgtctcg gcgtcgatga cctcgctctt cggcagcagg atcagcacgt
     4021 cgatgtcgga gcaggggtgc agctcgccgc gcccgtagcc gccgaccgcg atcaggtcga
     4081 cgtcgcgggc accggcgccg acgtgcagtt cccaggcgct gcgcaacacg acgtcgacca
     4141 tgcgcgcgcg gtcgcgcacc agcatctcga ccggctcgtc gtgctcgaag cgctgcctga
     4201 gggcggcatc gccgtcggcg agcgcctgcc ggaaggcggc gatcgccggc tctgacccgc
     4261 gcaacgcctg cggcagcgtc gccaggaacg accacggcgg ctgcggtgcg gtcggggcgg
     4321 gccccgagtc gagttcttcg taggcgaccg ggctgttcac gcgggcagtt gctcgcccgc
     4381 accgagcgtc aggacctcga agccgtcttc cgtgacgagc acggtgtgct cccactgcgc
     4441 cgacagcgag tggtcgcgcg tgatgacggt ccagccgtcc ggcagcagtc gcacgtcgcg
     4501 gcggccggcg ttgaccatcg gctcgatcgt gaacgtcatg ccgggctcga gcatcagccc
     4561 cgtcccggcc gtgccgtagt gcagcacctg cgggtcttcg tggaaaccac gaccgatgcc
     4621 gtgcccgcag tattcgcgca cgaccgaata gtggttggac tcgacgaagc gctggatcgt
     4681 cgcgccgatg tcgccgagcc tcgctccggg tcgcacctcg cggatgccga gccacatggc
     4741 ctggtaggtg aggtcgacga ggcgcacggc gtgcggcagc ggcttgccga catggaacat
     4801 gcggctggtg tcgccatgga agccgtcctt gatgaccgtg acgtcgacgt tgacgatgtc
     4861 gccctgcttg agcttgcgct cgcccgggat gccgtgacag accacgtggt tgaccgaggt
     4921 gcagatcgac ttcggaaaac cccgatagcc gagcggagcg ggcaccgcct gctgcacctg
     4981 gacgatgtgt tcgtggcaga gccggtcgag ttcctcggtc gtgacgcccg ggacgacgtg
     5041 ctggccgatc atgtcgagga cttcggccgc gagacggccc gcctcgcgca tggccgcctg
     5101 ttccgccgga cccttcagga gcacgcgtcc gctggcttcc gcggctgccg cggtggtggg
     5161 cgttggggaa tgttgcatca ggcgcatccg tggagacctc ggcccctcac tttgaccctt
     5221 tcccgcgggg gcgggtgagg gagcaagccc cccgcaggtg ggagagggcc gctgcgaggc
     5281 tccgtaagtg tttgaaaatc cgagggcgcg atggtataaa gcgcggcctt ttttgacaac
     5341 gaaatccgca cacgcaccgt cacgtgtggc tgggtgcccg gcaggccgtg aggctttcct
     5401 gggttgccgc atggggtgcg tggaggttca acccttcaag gagttttgcg aatggccggc
     5461 atgtccatgc gacagatgct ggaagcgggt gttcatttcg gacaccagac ccgcttctgg
     5521 aacccgaaga tgtccccttt catcttcggc gaacgtaacc gcatccacat catcaatctc
     5581 gagcagtccc tcccgctgtt ccaggaggcc gcgacgttca tcaggaacgt ggtcgccgac
     5641 ggcggcaagg tcctgttcgt gggtaccaag cgctcggcgc gcgaagcgat cgcggccgaa
     5701 gcgcagcgtt gcggcatgcc ctacgtgagc caccgatggc tcggcgggat gctcaccaat
     5761 ttcaagacga tccgccagtc gatccgtcgt ctcgccgaga tcgaggagat gcaggcgagc
     5821 ggcctgctgg agagccgcag caagcgcgag gcgcagatgg tgcgccgcga gaaggaaaag
     5881 ctcgaccgca gcctgggcgg catcaaggac atggaaggcc tgccggacgc gatgttcgtg
     5941 atcgacgtcg gccacgagaa catcgcgatc cacgaagcgc agaagctcgg catcccggtc
     6001 gtggcggtcg tcgacaccaa ctgctcgccc gacggcatca gctacgtgat cccgggcaac
     6061 gacgacgcga tgcgcgcgat ccagctctac gccgcgggca tcgccgatgc ggtcatcgaa
     6121 ggtcgctcga cgatcgtcga agtgccggtc ggtgaggacg aattcgtcga actcgacgag
     6181 gaaggcaagc cgcgtccccg taccggcggg ccccggcagc agccgccgcg cggccgcggc
     6241 accccggcgc gtcgcaagac gacgggcggt gcgggagcgc gtcgcaagcc ggtggccgcc
     6301 gaggacacga cggccgtcga gcagcagcag cgcgaggaac tcgtcgaggc cgatccggag
     6361 gagagcgccg aggcgttcat ggcccgccgc gaggcgtcgc gcaagaagcc ggcggccggc
     6421 gccacggccg caccggccgc ggccggcgaa cctgccacca ccgaggcggg cgcggcggaa
     6481 gcggccgcgg cagagcctgc ggccgccgcc gctgcaccgg aagcggcgaa gaccgaagga
     6541 gcctgagcca tggccgtcac ggcagaagca gtcaaggcgc tgcgcgagcg caccggcgcc
     6601 ggcatgatgg agtgcaagaa ggcgctggtc gaaaccaacg gcgacctcga ggccgccgcc
     6661 gagatcatgc gcaagtcggg cctcgccaag gccgacaaga aggccggtcg cgtggccgcc
     6721 gaaggcgtca tcgtagtcga gcgttccgcc gacggccggc aggctgcggt ggtcgagatc
     6781 aactccgaga ccgacttcgt cgcgcgccag gacgacttcc agtcgttcgc gggtgaggtc
     6841 gcccgcgctg cgcttacgca acggccggcc agcctcgagg ccctgctcga cacgcggctg
     6901 gcttcgggca agacggtcga ggagacgcgc cgcgagctga tcgcgcgcat cggcgagaac
     6961 atcggcgtgc gccgcttcgg gctggtcgaa gccgaaacgc cgctgcacac ctatcgccac
     7021 ggcacccgca tcggcgtggt ggtggcgatg cagggcggcg acgagacgct cgggcacgac
     7081 ctcgcgatgc acatcgcggc gatcaacccg cagtacctgt ccgccgacga ggtgcccgcc
     7141 gagtccgtcg cgaaggagaa ggaaatcttc gtctcgcagg tcgcgcagga cccgaagacc
     7201 gccggcaagc cggccgagat cgtcgcgaag atggtcgacg gcaaggtccg caagtacctc
     7261 ggcgagatca cgctgctcgg acagcctttc gtcaaggacg acaagcagtc ggtcgagcag
     7321 gtgctcaaga aggcaaacgc ccgcgtcgtg cgcttcgtgc gctacgaggt cggggcgggg
     7381 atcgagaaga agcaggagaa cttcgcagcc gaggtcatgg cgcaggtgcg gggcgcgtaa
     7441 gcccgcccgg ccaccgagcg actctctcag aaccccgcgc aagcggggtt ctttataatc
     7501 accgcaggcc actgggcgca gacgatggaa ccgaccgcag acccgcaacc gcgcaaccgc
     7561 cgcatcctcg tcaagctgtc cggtgaagcc ctgatgggcg atgccgacta cggcatcgac
     7621 cccgccttcc tgcgccgcct ggccaacgaa gtccgcagcg tccgcgagct gggcgtcgag
     7681 atcgcgctgg tcctgggcgg cggcaacctg ttccgcgggg ccgggctcgc gcgcgccggc
     7741 atggaccgcg tgaccggcga ccacatgggc atgctcgcga cggtcatgaa cgcgctcgcg
     7801 atgcaggacg ccctcgagtc gatgggcgtg gcgacgcgcg tgatgtcggc ggtgcgcatc
     7861 aaccaggtct gcgaggacta catccgccgc cgggcgatcc gccacctgga aaaaggccgc
     7921 gtgacgatct tcgccgccgg cacaggcaac ccgttcttca cgacggacac ggccgcgagc
     7981 ctgcgcgcga tcgagatcga ggccgacctg ctgctcaagg cgaccaaggt ggacggcatc
     8041 tacgacgccg atccggtcgg caacccgaac gcacggcgct acgaccggct gacctacgac
     8101 caggtgctgg accagcgcct gaacgtcatg gacgcgacgt cgatcgtgct gtgccgcgat
     8161 caccggctgc cgctgcgcgt gttcaacctg aacgacccgg gagcgctcgt gcgcgccgcc
     8221 agtggcgcgg acgtgggcac cctggtcacg atctgacgcg gccgaggccg cccgaagggg
     8281 agttgttcca tgctcgaaga catcaagaag gacgccaacg accgcatggg caagtgcgtc
     8341 gccgccttcc gccaggagct gaagcgcctg cgcacggggc gcgcgagcac gtcgctggtc
     8401 gaccacatcc gcgtggacta ctacggcgcg gagtcgccac tcaaccaggt cgccaacgtc
     8461 gcggtcgagg acgcgcgcac catcacgatc gtgcccttcg acaagcagat ggtctcgacg
     8521 atcgagaagg cggtcatgaa gtccgacctc ggcgtgacgc cgacgaccg
//
LOCUS       DASPFR010000197         3068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1155175, whole genome shotgun sequence.
ACCESSION   DASPFR010000197 DASPFR010000000
VERSION     DASPFR010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155175"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /gene="argH"
                     /locus_tag="VD737_05030"
     CDS             complement(<1..1019)
                     /gene="argH"
                     /locus_tag="VD737_05030"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HXW09956.1"
                     /translation="MSRLWDKGAPLDERVLRFTAGEDHALDDRLVQYDVQASVAHAEM
                     LHACGLLSQHDLAAIRTGLREIGEAHARGEWSVTLELEDGQTALETLLTQSIGDAGAR
                     LHAGRSRNDQVLAALRLYLRDAIEAMADSARAVVQSLEALAEAQGSIALPGYTHMQQA
                     MPSSVALWAQGYAAELHDDVDGLRHAHRRISKNPLGSAAGYGTPNLPIDRDETRTRLD
                     FAANHEPVTAVQLSRGKGEAEVLFQVALLMQDLGRLAADLLLFYTREFGFVQLPDAFT
                     TGSSIMPQKRNPDVFELVRGRTATAQACLAEVLAITAKLPSGYQRDLQLIKAPLFRSI
                     DVCLES"
     gene            complement(1137..2063)
                     /gene="argB"
                     /locus_tag="VD737_05035"
     CDS             complement(1137..2063)
                     /gene="argB"
                     /locus_tag="VD737_05035"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HXW09957.1"
                     /translation="MIGHKGEQAVTVRALRGAAPYIHMYKGKVFVIKAGGGAFRDAST
                     MRAFVEQVAILHHLGIRCVLVHGGGPQLDEVTAKLGVETRMVQGRRVTTAAAIDATSM
                     VLNGVINTQLLALCRAFGIAAIGLSGVDGGLVQARRRAPVTLASGEVVDYGMVGDIDA
                     VDGSVITRLLDAGFLPVVSPLSCDAGGQLLNINADTVAAAIGGALVAEKLILCTGAPG
                     ILERLDDPQSLISYTDVAGLRRLREEGAISDGMLPKSSAIEAAIRGGVRRVHVISYAD
                     SDALLAEIFTNEGKGTLVVADTKALTPAEQGK"
     gene            complement(2072..>3068)
                     /locus_tag="VD737_05040"
     CDS             complement(2072..>3068)
                     /locus_tag="VD737_05040"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HXW09958.1"
                     /translation="DRFLDLSDFTRDEIVDLLALAQRLQDRPEPRALEGRILGLLFLN
                     PSLRTLASFQAGMMRLGGSAFVVTPGAGTWNVETRDGVVMNGAAAEHVREAIPVLASY
                     CDALGIRAFAEGKDLAADLSEQQFLQMAGLCDKPLVNLESAVNHPCQALADWKTLDDL
                     HVPRAAKFVLSWVHHPRARPLAVPAATVHMAAQRGMEVVVLRPEGYALPAAIMRKARA
                     AAEASGGSVTETSDRASALAGSHVVYAKEWGSPEHYGDPEAEARARAVLADWCIDEHW
                     FAGARADCHLMHCLPVRRNVAVADAVLDGPRSRVIAEAANRMVVQMAVLHRLLKR"
BASE COUNT          451 a         1107 c         1039 g          471 t
ORIGIN      
        1 gattccaggc acacgtcgat cgagcggaac agcggcgcct tgatcagctg caggtcgcgc
       61 tggtaacccg aaggcagctt ggccgtgatc gccagcacct cggcgaggca ggcctgcgcc
      121 gtggcggtgc gtccgcggac gagctcgaac acgtccggat tgcgcttctg cggcatgatc
      181 gacgaaccgg tcgtgaacgc gtcgggcagt tgcacgaagc cgaactcgcg tgtgtagaac
      241 agcagcaggt cggcggccag ccggccgagg tcctgcatca gcagcgcgac ctggaacagc
      301 acttcggctt cgcccttgcc gcgggagagc tgcacggccg tcaccggctc gtggttcgcc
      361 gcgaagtcga gccgggtgcg cgtctcgtcg cgatcgatcg gcaggttcgg cgtgccatag
      421 cccgccgccg agccgagcgg attcttcgag atgcgtcggt gcgcgtgccg cagcccgtcg
      481 acgtcgtcgt gcaactccgc ggcgtaaccc tgcgcccaca gcgcgaccga gctcggcatc
      541 gcctgctgca tgtgcgtata gcccggcagc gcaatcgagc cctgcgcctc ggccagcgcc
      601 tcgagcgact ggaccacggc tcgcgccgaa tctgccatcg cctcgatcgc atcgcgcagg
      661 taaagccgca gcgcggcgag gacctggtcg ttgcgcgagc ggcccgcatg caaccgtgcg
      721 cctgcatcgc cgatgctctg ggtcaggagc gtctcgagtg ccgtctggcc gtcctcgagc
      781 tcgagcgtca ccgaccactc gccgcgcgcg tgcgcctcgc cgatctcccg gagcccggtc
      841 cggatcgccg ccaggtcatg ctgcgacagc agcccgcacg cgtgcagcat ctcggcgtgc
      901 gcgacggacg cctggacgtc gtactgcacc agccggtcgt ccagcgcgtg gtcctcgccc
      961 gccgtgaagc gcagcacccg ctcatcgagc ggcgcgccct tgtcccagag cctgctcatg
     1021 ccactgttct ccgtcgctat gagtgtctag cggttagcgg ttagcggtta gtaggaaccg
     1081 cgccgcgagg tgcgacgctc cgactaaccg ctgatcacta accgctaacc gcttcctcat
     1141 ttcccttgct ccgccggcgt cagtgccttg gtgtcggcga cgaccagcgt gcccttgcct
     1201 tcgttcgtga agatctccgc gagcagcgcg tcgctgtcgg cgtacgagat cacgtgcacg
     1261 cggcgcacgc cgccgcggat cgcggcctcg attgcgctcg acttgggcag catgccgtcg
     1321 ctgatggcgc cttcctcacg cagccggcgc agcccggcca cgtcggtgta cgagatcagc
     1381 gactgcggat cgtcgagccg ctcgaggatg ccgggggcgc ccgtgcacag gatcagcttc
     1441 tccgcgacca gcgcgccgcc gatcgccgcg gcgaccgtat ccgcgttgat gttgagcaac
     1501 tggccgcccg cgtcgcagga aagcgggctc acgaccggca ggaagccggc gtcgagcagg
     1561 cgcgtgatga cgcttccgtc aacggcgtcg atgtcgccga ccatgccgta atccacgacc
     1621 tcgccgctgg cgagcgtcac gggcgcccgc cgccgtgcct gcacgagccc gccgtcgacg
     1681 ccgctcaggc cgatcgccgc gatgccgaag gcgcggcaca gcgccagcaa ctgcgtgttg
     1741 atgacgccgt tcagcaccat gctcgtcgca tcgatggccg ccgccgtggt gacgcgccgg
     1801 ccctgcacca tgcgcgtctc gacgccgagc ttcgccgtaa cctcgtcgag ctgcgggccg
     1861 ccgccgtgga cgagcacgca acggatgccg aggtgatgca ggatcgcgac ctgctcgacg
     1921 aacgcgcgca tcgtcgaggc gtcgcggaac gcgccgccgc cggccttgat cacgaacacc
     1981 ttgcccttgt acatgtgtat gtacggcgcc gcgccgcgca gcgccctcac cgtgaccgcc
     2041 tgctctccct tgtgtccgat catcgtggtg ttcacctctt cagcagtcga tggagcacgg
     2101 ccatctgcac gaccatgcgg ttggcggcct ccgcgatcac gcggctgcgg ggcccgtcga
     2161 gcaccgcgtc tgcaacggcg acgttgcgac ggactggcag gcagtgcatc aagtggcagt
     2221 cggcacgcgc gccggcgaac cagtgttcgt cgatgcacca gtcggccagg accgcgcgcg
     2281 cccgcgcctc ggcctccggg tcgccgtaat gctcggggga gccccactcc ttggcgtaga
     2341 cgacgtgcga gcccgcgagc gcgctcgccc ggtcgctcgt ctcggtgacg ctgccgccgg
     2401 aggcctcggc cgccgcacgc gccttgcgca tgatcgcagc gggcaacgcg tagccctcgg
     2461 gtcgaagcac gacgacctcc atgccgcgtt gcgccgccat gtgcacggtc gcggccggca
     2521 ccgcgagggg ccgcgcgcgt ggatgatgga cccagctcag cacgaacttc gcggcccgcg
     2581 gcacgtgcag gtcgtcgagc gtcttccagt cggcgagcgc ctggcacgga tggttgacgg
     2641 ccgactccag gttcacgagg ggcttgtcgc aaagccccgc catctgcagg aactgctgct
     2701 ccgacaggtc cgcagcgagg tccttgcctt ccgcgaatgc gcggatgccc aacgcgtcgc
     2761 agtaggaggc gagcacgggg atcgcctcgc gcacgtgctc cgccgccgcg ccgttcatga
     2821 cgacgccgtc gcgcgtctcg acgttccagg tgcccgcgcc gggcgtcacg acgaacgcgg
     2881 agccgccgag acgcatcatg ccggcctgga acgacgcgag cgtgcgcagc gacgggttca
     2941 ggaacagcaa cccgaggatt cgcccctcga gtgcccgcgg ttccggccgg tcctgcaggc
     3001 gctgcgcgag tgcgagcagg tcgacgattt cgtcgcgcgt gaagtcggac aggtcgagga
     3061 agcgatcc
//
LOCUS       DASPFR010000198         4037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_544488, whole genome shotgun sequence.
ACCESSION   DASPFR010000198 DASPFR010000000
VERSION     DASPFR010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544488"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..754
                     /locus_tag="VD737_05045"
     CDS             221..754
                     /locus_tag="VD737_05045"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HXW09959.1"
                     /translation="MNDALYFVLNTLLTLVVVVFLLRVLMPLVRADFRNPVGEAVLKI
                     TNPLVLPLRRVFRPGRRVDVASIVALLIVQFAKTIVLLLVRGAALRPDTVLVAGLFGL
                     VILVIQFYFYAVLIYAIMSWFGGASYSPVGLVLGRLVEPLLAPFRRVIPPIGGLDLSA
                     LFLMIALQAVLILLRSP"
     gene            776..1075
                     /locus_tag="VD737_05050"
     CDS             776..1075
                     /locus_tag="VD737_05050"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HXW09960.1"
                     /translation="MTGAIRHDGPDLLLDVRVQPRASRTEFAGVMGDRLRIRLNAPPV
                     DGRANAALVEFVAAECGLARARVTLERGQAGRDKRLRLHGLATTPAALQRALATA"
     gene            1168..1698
                     /locus_tag="VD737_05055"
     CDS             1168..1698
                     /locus_tag="VD737_05055"
                     /inference="COORDINATES: protein motif:HMM:NF025822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4426 domain-containing protein"
                     /protein_id="HXW09961.1"
                     /translation="MTHRTMMARTMKTRTATRIAGVLAALVLGGTQLAGCDAGVDSRP
                     QTARPAEDTHQDFGNFEVHYNAVRTDQLTPDVARAYSIERSGNRVLLNVSMLAKSPDG
                     RTTPTDGVVKATAYNLNGQLKNLEMRRVQEGSSVYFIGEVGISGNEILVFDIDATPRD
                     GSGAYSVKFKREFFAD"
     gene            1836..2150
                     /locus_tag="VD737_05060"
     CDS             1836..2150
                     /locus_tag="VD737_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXW09962.1"
                     /translation="MAAKKSSAPTKSDILASISKDTGLSKKQVSSVFESLNGVIRKSL
                     KSNGMFTLPGLAKMKVVKKPATKAREGINPFTGEKMTFKAKPASKKVRVMPLKNLKGF
                     VN"
     gene            complement(2277..2999)
                     /locus_tag="VD737_05065"
     CDS             complement(2277..2999)
                     /locus_tag="VD737_05065"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXW09963.1"
                     /translation="MSQDDDADEDAASTTGDTPGAGFRPPVPQSLEIPGPAGPLEARL
                     EDPVPEGEPHPVVGVLCHPHPLHGGTMQNKVVHTAARAMQEAGAVTVRFNFRGVGRSG
                     GHYDEGRGEVDDALAVVEWLRGRYGADTLWLAGFSFGAAVALQAAVSAARPQRLVTIA
                     PPVGRIIKTPIAHPRCEWLIVQGDRDDLVDVAAVREWAAGYDPLPELLVVPDAEHFFH
                     GKLTELRSGVRTWLGGRGPVAG"
     gene            3118..3735
                     /locus_tag="VD737_05070"
     CDS             3118..3735
                     /locus_tag="VD737_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HXW09964.1"
                     /translation="MSSSFAHGQRIPAEFAFCAPDPVQHACLAPNRNPHLRWTGVPST
                     ARSLVLLCIDTDVPTRPDDVNREGRTVPADLPRTDFHHWVMVDIAPSVSEIGAGAASD
                     GVTPRGKRSPGGPAGSRQGINDYTGWFAGDAEMDGQYFGYDGPCPPWNDARLHHYHFV
                     LYATDLDRCPVDGAFTGPQVRAAIAKHVLAEARLTGTYTLNPAVR"
     gene            complement(3767..>4037)
                     /locus_tag="VD737_05075"
     CDS             complement(3767..>4037)
                     /locus_tag="VD737_05075"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXW09965.1"
                     /translation="WLYEHTAPDHKGKPFDSSRKGGRPFSFALGARQVIAGWDQGVAG
                     MRVGGQRRLVIPSALAYGDQGAGGVIPPNATLVFDVELLDVQPPP"
BASE COUNT          658 a         1411 c         1349 g          619 t
ORIGIN      
        1 cgcggcgtcg gacctcgaac cggcacggct ccgcgaacag gtcacctcgc gcggcgggac
       61 gaccgaggcg gcgatgcacg tgctcggggc cggcggcgtg cgtgatattt ttgcgcgggc
      121 cgtggcggcg gcggcccacc gctccggcga gctggcgcgg cagtacggcg ccgcctgaga
      181 acgagacgac caacgaagaa ggcagtacgg gaccccaacg atgaacgacg cgctgtattt
      241 cgtcctgaac acgctgctca cgctcgtggt ggtggtgttc ctgctgcggg tgctgatgcc
      301 gctggtccgc gcggacttcc gcaatcccgt cggcgaggcc gtgctcaaga tcacgaaccc
      361 gctcgtgctg ccgctgcgtc gggtgttccg gcccggccgc cgcgtcgacg tcgcatcgat
      421 cgtcgcgctg ctgatcgtgc agttcgcgaa gacgatcgtg ctgctgctgg tccgcggcgc
      481 ggcgctgcgt cccgatacgg tgctggtcgc cggcctgttc ggcctcgtga tcctggtgat
      541 ccagttctat ttctatgccg tgctgatcta cgcgatcatg agctggttcg gcggcgcgtc
      601 atacagcccc gtgggcctgg tgctcggccg gctggtcgag ccgctgctcg ccccctttcg
      661 ccgggtcatc ccgccgatcg gcggcctcga cctgtcggcg ctcttcctga tgatcgcgct
      721 gcaggcggtg ctgatcctgc tgcgctcgcc ctgagcgcac ctggcggccg cggtcatgac
      781 gggcgcgatc cgccacgacg gtcccgacct gctgctcgac gtccgcgtgc agccgcgcgc
      841 ctcgcgcacc gaattcgccg gcgtgatggg cgaccggctg cgcatccggc tcaacgcgcc
      901 accggtggac ggacgcgcca atgcggccct cgtggagttc gtcgctgcgg aatgcggcct
      961 ggcgcgcgca cgcgtgacgc tcgagcgcgg ccaggcgggg cgcgacaagc gactgcgcct
     1021 gcacggcctg gcgacaactc ctgccgcact gcaacgggcg ctcgcgaccg cgtgaccgcg
     1081 ttgcgcaccg tggcacgcgg catatagtgg ccgtgccgat cagtgctgca acgggcgacg
     1141 ttgcgcctgt gacgggagga tccacccatg acacatcgga cgatgatggc gaggacgatg
     1201 aagacaagga cggcgacccg cattgccggc gtgctggccg cactggtcct cggcggcacg
     1261 cagcttgcag gatgcgacgc gggcgtggac agccggccgc agaccgcgcg cccggccgaa
     1321 gacactcacc aggacttcgg caatttcgaa gtccactaca acgcggtacg caccgaccag
     1381 ctcacgcccg acgtcgcacg cgcatattcc atcgagcgca gcggcaaccg cgtgctgctc
     1441 aacgtgtcga tgctcgccaa gtcccccgat gggcgcacca cgccgaccga cggtgtggtc
     1501 aaggccaccg cgtacaacct gaacgggcag ttgaagaacc tggagatgcg gcgcgtgcag
     1561 gaaggatcgt cggtctattt catcggcgag gtcggcatca gcggcaacga gatcctcgtc
     1621 ttcgacatcg acgcgacgcc gcgtgacggc agcggagcgt actcggtcaa gttcaagcgc
     1681 gagttttttg cggactgatg cgccgcggca ctaaaaaaat aggaaaaaat cggtttcgac
     1741 gtattgagaa ccctagtatt catggtattt tcgcgctcgc tgagagacta accggggcca
     1801 gcggcccgca gcacacattt aggagacaca ctcagatggc agccaagaag agctctgcac
     1861 cgaccaagtc cgacatcctc gcttcgatct ccaaggacac gggcctgtcg aagaagcagg
     1921 tgagctctgt gttcgaatcg ctcaacggcg tgatcaggaa gagcctcaag agcaatggca
     1981 tgttcacgct tcccggcctc gccaagatga aggtcgtgaa gaagccggcc acgaaggcgc
     2041 gcgaaggcat caacccgttc acgggcgaga agatgacgtt caaggccaag ccggccagca
     2101 agaaggtccg cgtgatgccg ctcaagaacc tcaaggggtt cgtcaactga gcgacgcttc
     2161 cgcggcaggc tcgccgcgct gaggcacacg aaacgccggg gtgcgctgca tcccggcgtt
     2221 tttttacctg cgaccgcgga agaacccact agccactagc cactaaccac taaccactag
     2281 cccgccacgg gcccccgccc ccccagccac gtcctcacgc cgctccgtag ctcggtgagc
     2341 ttgccgtgga agaagtgctc ggcgtccggc acgaccagca actccggcaa ggggtcgtag
     2401 ccggcggccc actcgcgcac cgccgcgacg tccacgaggt cgtcgcggtc gccctgcacg
     2461 atcagccact cgcagcgcgg gtgggcgatg ggcgtcttga tgatgcggcc caccggcggc
     2521 gcgatcgtga cgaggcgttg cggccgtgcg gcggacacgg ccgcctgcag cgcgaccgcg
     2581 gcaccgaacg agaatccggc cagccagagc gtgtccgcgc cgtatcgccc gcgcagccac
     2641 tcgaccacgg cgagggcgtc gtcgacctcg ccccgcccct cgtcgtagtg gccgccgctg
     2701 cggccgacgc cgcggaagtt gaagcgcacg gtgaccgcgc cggcctcctg catcgcgcgt
     2761 gccgcggtgt gcacgacctt gttctgcatc gtcccgccgt gcaggggatg cgggtggcac
     2821 agtacgccga ccacggggtg cggctcgccc tcgggaacag ggtcctcgag ccgcgcttcg
     2881 agcgggcctg cgggccccgg gatctcgagt gattgcggca ccggaggtcg aaatccggcc
     2941 cccggcgtgt cgcctgtggt cgaagccgcg tcctcgtcgg cgtcgtcgtc ctggctcatg
     3001 actgtcagcg cctcgcggtt cgcaacatga cgacaccgag tctgccagag cgctgtcgcc
     3061 ccgaggcggc cgtggcagaa tcgcccgacg acaggacagg agattctcgt gcacctgatg
     3121 tcctcgagct tcgctcacgg ccagcggatt ccggccgagt ttgcgttctg cgcaccggac
     3181 ccggtccagc atgcgtgcct cgcgcccaac cgcaacccgc acctgcgctg gacgggcgtg
     3241 ccgtcgaccg cgcgctcgct ggtgctgctt tgcatcgata ccgacgtgcc gacccggccc
     3301 gacgacgtca accgggaagg ccgcaccgtc ccggcagact tgccgcgcac ggactttcac
     3361 cactgggtca tggtggacat tgcgccctcg gtcagcgaga tcggagcggg tgcagcttca
     3421 gacggcgtga cgccgcgagg caagcgcagc ccgggaggcc cggccggctc acgacagggc
     3481 atcaacgact acacgggctg gtttgccggc gacgccgaga tggacggtca gtatttcggc
     3541 tacgacggtc cgtgcccgcc gtggaacgac gcgcggctgc accactacca cttcgtgctc
     3601 tatgcgaccg acctggaccg ttgccccgtc gacggcgcgt tcacgggccc gcaggtccgc
     3661 gccgccatcg ccaaacacgt gctggccgag gcgcggctca ccggcacgta tacgctcaat
     3721 cccgcggtgc gctagcggcg gccgcgcgac gacgcccccg gccaggtcaa ggcggcggct
     3781 gcacgtcgag caactcgacg tcgaagacca gcgtggcgtt gggtggaatg accccacccg
     3841 ccccctggtc tccatacgcg agcgccgagg ggatcacgag ccggcgctgc ccgccgacgc
     3901 gcattccagc gacgccctgg tcccagcctg cgatcacttg ccgcgcaccc agcgcgaagc
     3961 tgaacggccg accgcccttg cgcgaactgt cgaacggctt gcccttgtgg tccggcgcgg
     4021 tgtgctcgta gagccag
//
LOCUS       DASPFR010000199        17136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_178136, whole genome shotgun sequence.
ACCESSION   DASPFR010000199 DASPFR010000000
VERSION     DASPFR010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17136
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178136"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..2115
                     /locus_tag="VD737_05080"
     CDS             331..2115
                     /locus_tag="VD737_05080"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HXW09966.1"
                     /translation="MADVFVSYARSDRARVAPIVAAIEAEGWTVWWDPDIAAGQEFDR
                     RIADELKGASAVLVVWTPRSVESRWVRGEARHGVERGILVPVRFDAAELPIDVRALHT
                     TDLDDWHGDPAAPQAREVLRALRATIERQRALQTAATSGRAALAASAAVGRPDRIPIC
                     VLPFANMSGDPEQEYFSDGITEDIITDLSKVSALSVISRNSAFVYKGRHVDLPKVARE
                     LNATHILEGSVRKAGGRVRITAQLVEAATNDHLWAERFDRDLHDIFELQDEISGAIVK
                     ALRLRLLPEEKRAIEQRGTDDVDAYNLYLMARQLYLTGNEGDSRRADAVVRLCTRATE
                     LDPAYARAWALRALGEQLGRFAAGTGSDGGLASAERALALDADLAEAHAVKATILAGE
                     GRHDAAALEIAQALRLDPESSEVNRAAGYLSYRQHRFGVAIGHWEKAAAVNETDINSL
                     GMLLSCYRAVGDVEALHRTARRTLDRTEKVLAHDPSNGTVLGYSVYAWAALGEAERAK
                     ERMNRALLVDPDNLNMRYNFGCVLVIHLPDHEAALDMLEPFFEKVAEGFFEHARVDPD
                     FEAIRDLPRFKAMVAKAEERLARARSAT"
     gene            complement(2118..2960)
                     /gene="fghA"
                     /locus_tag="VD737_05085"
     CDS             complement(2118..2960)
                     /gene="fghA"
                     /locus_tag="VD737_05085"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HXW09967.1"
                     /translation="MEVLSEHSCFGGVQRFVRHQSRECGGPMRVAVYEPPQAREHGAR
                     VPVLYYLAGLTCTEETFTIKAGAQRLAAQYGLLLVAPDTSPREPRIPGDDASWDFGVG
                     AGFYVDATEAPWSSHYRMYSYVTRELPELIAGSFPVDASRHSIMGHSMGGHGALVCAL
                     RNPDLYRSVSAFAPIVAPSQVPWGQKAFRGYLGPERTRWADYDATALVERNPWPGKIM
                     IDQGTDDKFLAEQLRPELFADACARSGQQLRLRMQPGYDHSYWFIQTFMADHVAWHAA
                     ALGV"
     gene            complement(3087..4196)
                     /locus_tag="VD737_05090"
     CDS             complement(3087..4196)
                     /locus_tag="VD737_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HXW09968.1"
                     /translation="MKTRAALCHQAGQPLVIETVDLDGPRAGEVLVEIRATGVCHTDE
                     FTRSGADPEGLFPVIFGHEGAGVVVDVGAGVKSVSRGDHVIPLYTPECRECKSCTSRK
                     TNLCTAIRATQGQGVMPDGTSRFSLGGEKIHHYMGCSTFSNYTVLPEIAVAKVREDAP
                     FDKICYIGCGVTTGIGAVINTAKVEPGASVVVFGLGGIGLNVIQGARMAGAAMIVGVD
                     INPARRSLAEKFGMTHFVNPKEVEGDLVPYLVSLTGGGADYSFECVGNVDLMRQALEC
                     CHRGWGVSVIIGVAGAGQEIRTRPFQLVTGRVWKGTAFGGARGRTDVPKIVDWYMNGK
                     VWIDPLITHTMPLERINDAFHLMHSGESIRSVVTF"
     gene            4356..5018
                     /locus_tag="VD737_05095"
     CDS             4356..5018
                     /locus_tag="VD737_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09969.1"
                     /translation="MNFPSRCLLTLAYAWSAVAFGASGGDISKVIGSVRVESGQAARD
                     VSTVNGSVTIGATSTVEAVETVNGSVRIEERAVVESAETVNGDVTIGDGAEVREDVET
                     VNGDITLRSGARIGGSVANVNGDMLIEGASVGGGLETVNADIRLTSGARVSGGIHVER
                     NNGNGRSWFGKEQSNPKITVEQGAVVEGPLVFEREVDLYVAPGVTLPAVQGVQPKRYT
                     LQ"
     gene            complement(5088..6644)
                     /locus_tag="VD737_05100"
     CDS             complement(5088..6644)
                     /locus_tag="VD737_05100"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HXW09970.1"
                     /translation="MVVAACGGGGGGSDGGGDGGGTPPPPPPPPPPALELPTDTTSSE
                     SAYLTSTSSGWEVVPLLTVGDSPASGTYPLLGKPDGLGAFAGRLSEAGEVTDTGDYLT
                     VLMNHEMRDDQGVARAHGTRGAFVSQWTFELDTMRASAGRDLGTHVWMYSGGGWADQT
                     GNVTFDRLCSATLAPRSAFFNSATGRGYDGRIFTNGEEVDDVGRAFAWIVGGPEHGRV
                     YHLPHLGQLAFENVVPNPGSGDTTITVGLDDVQAGQVYVYVGQKSSTGNPVERAGLHG
                     GRLYGLRVTGGGPNYAPGPVPRENRGAIDGTFELVDVSDVAAGSGFELQSASDARGVT
                     AFARPEDGAWDPQNSRVFYWVTTGSFMDGVRQSPRLYRLTFDSLENPTGGTIDLVVDA
                     ASLSGTDGLPADGFDNITVAADGRVVVQEDGGNDDYVSKIWVVDPGTGTATQAFESDR
                     SRFLAGGSNFLTSDEENSGVIEVTDQVQGASWYDSGRRYFLGTNQAHYRHPTTSLVEG
                     GQLYLFASPK"
     gene            complement(6777..7745)
                     /locus_tag="VD737_05105"
     CDS             complement(6777..7745)
                     /locus_tag="VD737_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HXW09971.1"
                     /translation="MDRSERFYKIHGMLQNRKVVRTQEFLDELEVSKATFKRDLEYLR
                     DRMGAPIVYDRHEEAYRYDMGVADRELWQLPGLWFTAEEILALLTMDKLLADLQPGVL
                     GETVAPLRKRLKSMLASQRHSAEDIAQRIRILAMGSRKVDPAHFRVLSTALLSRRRLR
                     IRHLARRTGEVSEREVSPQRLVHYRDNWYLDAWCHKRQALRTFGLDAIESATVTDKAT
                     RDVAEDTLDRHFASGYGIFAGSETQEAVLLFSESRARWVSRELWHPGQDGKMQLDGSY
                     LLRFPYAQEPELVMDILKYGPDVQVLAPDSLREAVATKLRAAAALY"
     gene            complement(7775..8917)
                     /locus_tag="VD737_05110"
     CDS             complement(7775..8917)
                     /locus_tag="VD737_05110"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HXW09972.1"
                     /translation="MTARVVLVPRGPRLRRMLLAVVGVVAGFASAMTAQAAEVLDEAA
                     AARFAALALQCVHLEYPNKLAHTLASDADARPPRQLTPAFYGCYDWHSSVHGHWLLVR
                     LLRQFPAASFATAARAALARSLTTENVLGEVAYLKTPGRASFERPYGLAWLLALAGEL
                     RQWDDPQARQWSTTLRPLEQEAAARLAAWLPKLRYPIRVGEHSQTAFAFGLVHDWART
                     AGDDAMRSLVEQRSVAFYAADRDCPLAYEPSGEDFLSPCLAEADLMRRVLPSPAYAKW
                     LHGFLPSIPRDARARWLEPGVVSDRSDPKLAHIDGLNLSRAWMLHGIASGLAPNDRRI
                     PALEAAAGVHAAAALPAVTGEHYEGGHWLGTFAVYLTTGAGRAAGR"
     gene            complement(8935..10575)
                     /locus_tag="VD737_05115"
     CDS             complement(8935..10575)
                     /locus_tag="VD737_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HXW09973.1"
                     /translation="MSRRRTLLPVLCLLALAGCRGTPATMPAAASDGSATPAAAAAPP
                     AAGARWWNDRVFYEIFVRSFADSTTGPLADDGIGDFQGLIEKLDYLNDGDPATTTDLG
                     ITGIWLMPISPSPSYHGYDVTDYYGIHPQYGTRADFERFIAECHRRGIKVITDLVLNH
                     SSSRHPWFVKALAGDQQFRDRYIFVDANAVPDVRGPWDQQVWQEARGQHYYGIFWSGM
                     PDLNYRNPAVTAEAYRVAEFWLRDLGVDGFRLDAIRHLIEDGDVMSDTPETIEWLRGF
                     RTHVKHVKPGAMMVGEVWTATEVVSEYIERGALDLAFEFDVAKAILQSIQSGRRDDLA
                     YALQNAWDAYPGQQFATFVTNHDQDRIASVLGEEPARLELAASLLLAAPGVPFLYYGE
                     EIGQVGRKPDEMIRNPMPWTSGPNGGFTRAARPWEPLQRGHERRNVATQSADPASLLA
                     HYRRWIRLRQAEPALLQGDFTLLDLGRDDVIGWQRSAQGRTLTFIANLGRDELRGLVL
                     PAAVAATVAVDRLTGRPVDARIALRLEPYGVRLYTSAR"
     gene            10748..11455
                     /locus_tag="VD737_05120"
     CDS             10748..11455
                     /locus_tag="VD737_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW09974.1"
                     /translation="MQHAAELQFDAGVRRAESVCLTADEFVIDRSLRGARRGPTLTVA
                     HRHRRCESELALLDGHRLAVRDRRREVVRRYVVDLRFIADAPVTIRTVAWNCWLASFA
                     SLATAGLAAWLAWAQRAHPQAWSAWVVALGLAATALLLGAVALYRTYETTLLFSVDGH
                     VTLARLTGALGSTRHAAAFHAELTRGIAAARAAIAQSRQAFLRDELREHRRLYEEGVL
                     PADAYEAGKRRILQAHD"
     gene            complement(11673..14402)
                     /locus_tag="VD737_05125"
     CDS             complement(11673..14402)
                     /locus_tag="VD737_05125"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXW09975.1"
                     /translation="MTIPSAPAVAAAAVAAPVDQLIPFDRFQLDNGLTVIVHTDRKAP
                     IVAVNLWYHVGSKNETPGRTGFAHLFEHLMFQGSEHFNDEFFRPLELVGATSMNGTTN
                     FDRTNYFQNVPTTALDLALWLESDRMGHLLGVVDQARLDEQRGVVQNEKRQGENRPFG
                     LVPETVFRASFPPGHPYRTLPIGSMEDLDAATLDDVRDWFKTYYGAANAVLVLAGDID
                     VATARAKAQQYFGHIAPGPALTRPGEWIAARTESKRETMYDQVAQPRWQRYWNTPPDG
                     SADAEHLALAAEILGGGKTSRLYERLVYRERLADTVGAGQSPLEIAGLFSISADVKTG
                     VPVERVEAAVEEELRRFIEQGPAAAELDRAKTVFRSSFIRGLERIGGFGGKADVLASC
                     EVYEGDPGCYRRSLRTIAAATPADLQQVARRWLMQGDYTLTVLPQPPYKAAATSAVDR
                     SKGPPTITSFPDVAFPDLQRRKLSNGIPVIVATRPGVPVARVSLLFDAGYVADLGRKP
                     GTSSFAMGMLDEGAGQFDALGLADRVERLGADLSAGSALDTSFVAVSTLTEKLEPSLQ
                     LLADVVRRPTFPTAEIERIRKEWIAGIAREKTSPDALALRVLPPVMYGDGHPYAIPFS
                     GSGTEESVAALTREDLVDFHRQVLRPDNVTLIVTGAVTPEQVLPLLERQFGDWRPEPG
                     VSLVRPAIPLSPPAAAPRVFLLDRPGAQQTTLLVGQLMPSSRAEDRLELNTANAVVGG
                     TFTSRLNMNLREQKHWSYGVRSSLPEALGQRPWLLSAPVQSDKTLEAIAEIRRELEQF
                     VGPSPATADEIAKIRQRDVRALSGQYETNASVSGAIADIVRFGRPDDWVRTLRERLEA
                     QTDAGVQQAAQRAFRPNALTWVIVGDLGRIEAPIRELGLGEVQVLDADGEVLR"
     gene            14726..15877
                     /locus_tag="VD737_05130"
     CDS             14726..15877
                     /locus_tag="VD737_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HXW09976.1"
                     /translation="MSSRYLISLAVAAALAAPVAAQAEQGDWLFRVGLSVTNPDSTNL
                     ELPPVGDIVIDDDISPTFNISYFLTDHISTELLAAWPFTHGVDLKTGGGGDDKTRIGN
                     VDHLPPTLSLNWHFNPDGTFRPYIGVGANYTLFSSEETRGPLTGSILKLDDSFGAAGQ
                     VGVDIGSGNWFFNANARYIQIESDATLDGADIGTIDVNPWVYGVHIGYKFGRPAPVAE
                     PEPAPAAAPPPPPPPPPPPPPADADGDGVTDDLDKCPGTPAGTKVDKVGCPLEQTLKL
                     LFDFDSAELRPESITELERVVKFMNDVPFAMVLVQGHTDSVGGDAYNLALSDRRAKAV
                     FDYLSSRGVDPARVKSEGKGEAEPIADNKTAEGRQENRRVMLIRTDSGM"
     gene            16062..16931
                     /locus_tag="VD737_05135"
     CDS             16062..16931
                     /locus_tag="VD737_05135"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXW09977.1"
                     /translation="MKPVHALALMTALAVPPAFGADTATDTGAAGADAALQAAVNGDW
                     RSADEKSRDRYRHPVESLTFWGLKPGMTVLEVQPGGGWWTNILAPYARATGGKLYVTA
                     ADLQNPKLSDAAKKARADFEARYAARPETFGKLDYVDWGAVSRSLPANTFDLILTARS
                     VHGWMQQGPDVAQQTFAEFHEALKPGGILAVEQHRAHPKNGAAEDPKSGYVSEQHVID
                     LAQKAGFKLAAKSEINANPKDTKDHPFGVWTLPPALYSTPYGSGQPDDPKFDHSKYLA
                     IGESDRMTLKFVK"
BASE COUNT         2707 a         5875 c         5941 g         2613 t
ORIGIN      
        1 gtagcagttg cccgagacct gcatgtggtg aagcgtgggc atggactgat tccccgatcg
       61 atggctacgg ctgcggcgac tggcctgacg tcgctgcgcg gcgctggcag gtgcagccgc
      121 catcgaagtg agccgtcgcg gcgttcatga cgagcgcgtc cccgcagtct tgacgttcga
      181 gttgccggtc cacatgacgc tgccccccac gtgagctccc gctgggcaaa tggagcaccg
      241 ccgacgccac tcgtcaactg ccgcatcggt atcccacgca cggcgggagc aagtaagctg
      301 gagcccttcg acaccgcagg gtcatggacg atggccgacg tcttcgtctc ctacgcccgc
      361 agcgaccggg cgcgcgtcgc gccgatcgtg gccgcgatcg aggcggaagg ctggacggtc
      421 tggtgggatc cggacatcgc cgccggccag gaattcgacc gccgcatcgc cgacgagctc
      481 aagggtgcgt cggccgtgct ggtggtctgg accccgcgct cggtcgagtc tcgctgggtt
      541 cgcggcgagg cgcgtcacgg cgtcgagcgt ggcatcctcg tgccggtgcg attcgacgcc
      601 gccgagctgc cgatcgacgt acgcgccctg cacacgaccg atctcgacga ctggcatggg
      661 gatccggcgg cgccgcaggc acgcgaggtg ctgcgcgcgc tgcgcgcgac gatcgagcgc
      721 cagcgcgcgt tgcagaccgc ggcaacgtcg ggccgggccg cgctcgcagc cagcgctgcg
      781 gtcggcaggc ccgaccggat cccgatctgc gtgttgccgt tcgcgaacat gagtggcgac
      841 cccgagcagg agtacttcag cgacggcatc accgaggaca tcatcaccga cctcagcaag
      901 gtgtcggcgc tctcggtgat ctcacgcaac agcgcgttcg tctacaaagg aaggcacgtc
      961 gacctgccga aggtggcgcg cgagctcaac gcgacgcaca tcctcgaggg cagcgtgcgc
     1021 aaggccggcg gccgcgtgcg gatcacggca cagctggtcg aagcggcgac caacgatcac
     1081 ctgtgggccg agcgctttga ccgcgacctg catgacatct tcgagctgca ggacgagatc
     1141 tccggagcga tcgtcaaggc gctgcgcctg cgtctgctgc cggaggagaa gcgtgcgatc
     1201 gagcagcgcg gcaccgacga cgtcgacgcc tataacctgt acctgatggc ccggcagctc
     1261 tacctcaccg gcaacgaggg ggattcgcgg cgcgcggatg cggtcgtgcg cctgtgcacc
     1321 cgggcgaccg agctcgaccc cgcttatgcg cgcgcgtggg cgctgcgggc gctcggcgag
     1381 cagctggggc ggttcgccgc cggaacgggc agcgacggcg gcctggcgtc cgccgagcgg
     1441 gcgctcgcac tggacgcaga ccttgcggag gcccacgccg tcaaggccac catcctggcc
     1501 ggagaaggcc gccacgacgc cgccgccctg gagatcgcgc aggcgttgcg tctcgacccg
     1561 gagtcgtccg aggtcaatcg cgctgcgggc tacctcagct accgccagca ccgtttcggc
     1621 gtagcgatcg gccactggga gaaagccgcc gcggtcaacg agacggacat caactcactc
     1681 ggcatgctgc tcagctgcta ccgggccgtc ggcgacgtcg aggcgctgca ccgcacggca
     1741 aggcggacgc tcgaccgcac cgagaaggtg ctggcgcacg atccgagcaa cggcacggtg
     1801 ctcggctaca gcgtctacgc gtgggcggcg ctcggtgagg ccgaacgggc caaggagcgg
     1861 atgaaccggg cgttgctggt cgatcccgac aacctcaaca tgcggtacaa cttcggctgc
     1921 gtactcgtga ttcacctgcc cgaccacgaa gcggcgctcg acatgctcga gccgttcttc
     1981 gagaaggtgg cggagggttt cttcgaacac gccagggtcg atcccgattt cgaggcgatc
     2041 cgcgacctgc cgaggttcaa ggcgatggtc gcgaaggccg aggaacggct cgcgcgcgca
     2101 cggtcagcta cctgacctca cacacccagt gccgccgcgt gccacgccac gtggtccgcc
     2161 atgaacgtct ggatgaacca gtagctgtgg tcgtagcccg gctgcatgcg cagccgaagt
     2221 tgctggccgg acctcgcgca ggcgtccgcg aacagttccg gccgcagctg ctcggcgagg
     2281 aatttgtcgt cggtcccctg gtcgatcatg atcttgccgg gccacgggtt gcgttccacg
     2341 agtgccgtcg cgtcgtaatc ggcccaacgc gtccgctcgg ggccgagata gcccctgaac
     2401 gccttctggc cccagggcac ctgcgagggt gcgacgatcg gggcgaaggc cgacacggac
     2461 cggtagagat cgggattgcg cagcgcgcac acgagcgcgc cgtgaccgcc catcgagtga
     2521 cccatgatcg agtgtcgcga tgcgtccact ggaaaactgc ccgcgatcag ttccggcagc
     2581 tcccgcgtca cgtagctgta catgcgatag tgcgacgacc acggtgcctc ggtcgcgtcg
     2641 acgtagaagc cggcgcccac cccgaagtcc cagctcgcgt cgtcacccgg aatccgcggc
     2701 tcgcgtggac tcgtgtcggg cgccaccagc agcaggccgt actgcgccgc gaggcgttgg
     2761 gcgcccgcct tgatcgtgaa cgtttcctcg gtgcaggtga gcccggcgag gtaatagagc
     2821 accggcaccc gcgcgccgtg ctcgcgcgcc tgcggtggct cgtacaccgc gacccgcatc
     2881 ggcccgccgc actcgcgcga ctggtggcgg acgaagcgct gcacgccgcc gaagcaggaa
     2941 tgctcggaga ggacttccaa tgtcggactc cagcgaaagg agtgtttagt ggctagtggc
     3001 tagtggctgg tggctagtga agatccggaa cggccgcaat acggccttct gcactggcca
     3061 ctagccactg gccactgacc acttcctcag aacgtcacca cgctgcggat cgactcgccg
     3121 ctgtgcatca ggtggaacgc gtcgttgatg cgctcgagcg gcatggtgtg cgtgatcagc
     3181 gggtcgatcc agaccttgcc gttcatgtac cagtcgacga tcttcggcac gtcggtgcgg
     3241 ccgcgcgcgc caccaaaggc cgtgcccttc cagacgcgac cggtgacgag ctggaatggc
     3301 cgcgtgcgga tctcctgtcc ggcgccggcc acgccgatga tgaccgacac gccccagcca
     3361 cggtgacagc attcgagcgc ctggcgcatc aggtcgacgt tgccgacgca ctcgaagctg
     3421 taatcggcgc cgccgcccgt gagcgacacg aggtacggca cgaggtcgcc ttcgacctcc
     3481 ttcgggttca cgaagtgggt catgccgaac ttctcggcga gcgagcgtcg cgccggattg
     3541 atgtcgacac ccacgatcat ggcggcgccc gccatgcgcg cgccctggat cacgttgagg
     3601 ccgatgccgc cgaggccgaa gacgacgacg ctggcgccgg gctcaacctt cgcagtgttg
     3661 atgacggcgc cgatgcccgt cgtcacgccg cagccgatgt agcagatctt gtcgaacggc
     3721 gcgtcttcgc ggactttcgc aaccgcgatc tcgggcagca cggtgtagtt cgagaacgtg
     3781 gagcaaccca tgtagtggtg gatcttctcg ccgcccaggg aaaaccggct ggtgccgtca
     3841 ggcatcacgc cctggccctg cgtcgcgcgg atcgccgtgc agaggttggt cttgcgcgac
     3901 gtgcagctct tgcactcgcg acattcgggc gtgtagagcg ggatcacgtg gtcgccccgg
     3961 gagaccgact tcacgccggc acccacgtcc acgacgacgc cggcgccctc gtgcccgaag
     4021 atcaccggga acagcccttc ggggtccgcg ccggagcgcg tgaactcgtc cgtgtggcag
     4081 acgcccgtcg cgcggatctc gaccagcact tcgccggcgc gcggcccgtc gaggtcgacg
     4141 gtctcgatca cgagcggctg gccggcctgg tggcagaggg cggcgcgtgt cttcatgggc
     4201 ggctccgtag gtgcagctgt cgccggcgcg gaagcgcgga gcctagcgca acgtggcggt
     4261 cgttgcaggc ggtcgagcgc agcggttgca accttccgcg cccgccatgc atcccaaggg
     4321 cacacgagca cgaccgactc gccggagacc gcgccatgaa ctttccgtcc cgttgcctcc
     4381 tgaccctggc gtatgcgtgg tcggccgtcg cgttcggcgc gtctggcggc gacatcagca
     4441 aggtgattgg cagcgtgcgg gtcgagtccg gccaggcggc ccgcgacgtc tccaccgtga
     4501 acggttcggt gaccatcggg gcgacgtcga ccgtcgaggc ggtcgagacg gtcaacggct
     4561 cggtccgcat cgaggagcgc gcggtggtcg agtcggcgga aaccgtcaac ggcgacgtga
     4621 cgatcggcga cggggccgag gtccgcgagg acgtcgagac ggtgaacggc gacatcaccc
     4681 tgcgcagcgg cgcgcgcatc gggggatcgg tcgccaacgt caacggcgac atgctgatcg
     4741 aaggcgcgag cgtcggcggc ggactcgaga cggtcaatgc cgatatccgc ctgacctccg
     4801 gggctcgcgt atccgggggc atccacgtgg aacggaacaa cggcaacggc cgctcgtggt
     4861 tcgggaagga gcagagcaac ccgaagatca ccgtcgagca gggcgccgtc gtcgagggtc
     4921 cgctcgtatt cgagcgcgag gtggacctgt acgtggcccc cggcgtcacg ctgccggccg
     4981 tgcagggcgt gcagccgaag cgctatacgc tgcagtaaat caccggcaga cagggagagg
     5041 gatcggccga gtccgccccg accgatcccg ggtttacccg ccgcgcctca cttgggcgac
     5101 gcgaacaggt aaagctggcc tccctcgacg agcgacgtgg tggggtgccg gtagtgcgcc
     5161 tggttggtgc cgaggaaata cctcctgccg ctgtcgtacc agcttgcccc ctgcacctgg
     5221 tcggtcacct cgatcacgcc cgagttctcc tcgtcgctcg tcaggaagtt cgaaccaccg
     5281 gcgaggaaac ggctgcggtc cgattcgaaa gcctgcgtgg ccgtgcccgt gcccggatcc
     5341 acgacccata tcttgctcac gtaatcgtcg ttgccgccgt cctcctgcac gaccacgcgc
     5401 ccgtcggcgg cgacggtgat gttgtcgaag ccgtcggccg gcaggccgtc ggtgcccgag
     5461 agcgaggcag catcgacgac gaggtcgatc gtgccgcccg tcgggttttc cagcgagtcg
     5521 aacgtgaggc ggtacaaccg cggcgactgg cggacgccgt ccatgaacga acccgtcgtg
     5581 acccaataga agacgcgcga attctggggg tcccaggcgc cgtcctccgg cctggcaaaa
     5641 gccgtgacgc cgcgcgcgtc cgaggccgac tgcagctcga agccggaacc cgccgcgacg
     5701 tcgctcacgt cgacgagctc gaaggtgccg tcgatcgcgc cccggttctc cctcggcacc
     5761 ggaccggggg cgtagttggg acccccgccc gtgacccgca gcccgtacag cctgccgccg
     5821 tgcagccccg ctcgctcgac cgggttgccg gtggaggatt tctggcctac gtagacgtag
     5881 acctgtcccg cctgcacgtc gtccaggccg accgtgatcg tggtgtcgcc ggaaccgggg
     5941 ttcggcacga cgttttcgaa cgcgagttgc ccgaggtgcg gcaggtggta cacgcgaccg
     6001 tgttccggcc cgccgacgat ccaggcgaac gcgcgcccca cgtcgtcgac ttcctcgccg
     6061 ttggtgaaga tgcgcccgtc gtagccgcga cccgtcgcgg agttgaagaa tgccgaccgc
     6121 ggcgccaggg tcgccgagca caggcggtcg aacgtcacgt tgcccgtctg gtcggcccag
     6181 ccaccgcccg agtacatcca cacgtgcgtg ccgaggtccc gcccggcact ggcgcgcatc
     6241 gtgtcgagtt cgaaggtcca ctgcgagacg aatgcgccgc gcgtcccgtg cgcgcgggcg
     6301 acaccctggt cgtcgcgcat ctcgtgattc atcaggaccg tcaggtaatc gccggtgtcc
     6361 gtcacctcgc ctgcctccga aagccggccg gcgaaggcgc cgagcccgtc gggtttgccg
     6421 agcagcgggt aggtgccgct cgcgggggaa tcgccgaccg tcagcagggg gaccacttcc
     6481 caaccgctcg aggtcgaggt caggtacgcg ctctctgagc tcgtcgtgtc ggtcgggagc
     6541 tcgagcgccg ggggtggtgg tgggggaggc ggcgggggcg tgccgccgcc gtcaccgccg
     6601 ccgtcgctgc cgccaccgcc gccgccgcag gcggcgacca ccacggtcgt caagaatccc
     6661 agaacgctca gccggcctac cgtggcggca aatcgatgtc gcatgttcga cccctttccc
     6721 tgggctcgtc tttggaaaaa attacagcgg cgccaaccgc gcgctccgct ggctcctcag
     6781 tagagcgccg cggcggcccg aagtttcgtg gccaccgcct cgcgcagcga atccggagcg
     6841 agcacctgca cgtccggccc gtacttgagg atatccatca cgagttcggg ctcctgggcg
     6901 taggggaaac ggagaagata gctgccgtcg agttgcatct ttccgtcctg cccaggatgc
     6961 cagagctctc gcgacaccca gcgcgcccgg gactcggaaa acagcagcac ggcttcctgc
     7021 gtctcgctgc cggcgaagat cccgtagccg ctcgcgaagt ggcggtcgag cgtgtcttcg
     7081 gcgacgtccc tggtggcctt gtcggtcacg gttgccgact cgatcgcgtc gaggccgaac
     7141 gtgcgcagcg cctggcgctt gtggcaccac gcgtccaggt accagttgtc tcgatagtgg
     7201 acgagccgtt gcggcgacac ctcgcgctcg gacacctcgc cggtgcgccg ggcgaggtgg
     7261 cggatgcgca ggcgccgccg cgagagcagc gccgtgctga gcacgcgaaa gtgcgccgga
     7321 tcgaccttgc gcgagcccat cgccaggatg cggatgcgct gcgcgatgtc ctccgcgctg
     7381 tgacgttgcg aggcgagcat cgacttgagc cgcttgcgca gcggcgcgac ggtttcgccc
     7441 agcacgccgg gctgcaggtc ggcgagcagc ttgtccatgg tcagcagcgc gaggatttcc
     7501 tccgccgtga accagagtcc cggcagttgc cacagctcgc ggtcggccac gcccatgtcg
     7561 tagcgatagg cttcctcgtg ccggtcatag acgatcggcg cgcccatgcg gtcgcgcagg
     7621 tattcgaggt cgcgcttgaa cgtggccttc gagacctcca gctcgtcgag gaattcctgc
     7681 gtgcgcacca ccttgcggtt ctgcagcatg ccgtggatct tgtagaagcg ttccgacctg
     7741 tccatccacc atcccgtcgt cgtcgtatcg tggttcagcg tccggcggcc cgacccgcgc
     7801 ccgtcgtgag gtacacggcg aacgtgccga gccagtggcc gccctcgtaa tgctcgccgg
     7861 tgaccgccgg cagcgccgcg gctgcatgca cgcccgccgc ggcttcgagg gcggggattc
     7921 gacgatcgtt cggcgcgagg ccgctggcga tgccgtgcag catccaggcc cggctcaggt
     7981 tcagtccgtc gatgtgcgcg agcttgggat cgctgcggtc ggagacgacg ccgggctcga
     8041 gccagcgggc gcgcgcgtcg cgcgggatcg agggaaggaa gccgtgcagc catttcgcat
     8101 acgccggcga gggcagcacg cgtcgcatca ggtcggcctc ggccaggcag ggcgacagga
     8161 agtcctcgcc ggacggctcg taggccagcg ggcagtcacg gtcggccgcg tagaacgcga
     8221 ccgagcgctg ttcgaccagc gagcgcatgg cgtcgtcgcc ggccgtacgt gcccagtcgt
     8281 gcaccaggcc gaacgcaaac gcagtctggc tgtgctcgcc gacgcggatc gggtagcgca
     8341 gcttcggcag ccaggccgcc agccgcgcgg cggcttcctg ctccagcggc cgcagcgtcg
     8401 tcgaccactg ccgcgcctgc gggtcgtccc actggcgcag ctcgcccgcg agcgccagca
     8461 gccacgcgag accgtagggg cgctcgaacg aggctcgacc gggcgtcttc aggtaggcga
     8521 cttcgcccaa gacgttctcg gtggtcaggc tgcgcgcgag cgctgcgcgc gccgccgtcg
     8581 cgaacgatgc cgcaggaaac tgccgaagca ggcgcacgag cagccagtgg ccgtgaaccg
     8641 acgagtgcca gtcgtagcag ccgtagaacg cgggcgtcag ctgccgcggc gggcgcgcgt
     8701 cggcgtcgct cgcgagcgtg tgcgcgagct tgttcgggta ttcgaggtgc acgcactgca
     8761 gcgcgagcgc cgcgaaacgc gcggccgcgg cttcgtcgag tacctcggcg gcctgcgccg
     8821 tcatggcgct cgcgaagccg gcgacgaccc cgacaaccgc gagaagcatg cggcgcagcc
     8881 gcggaccgcg cggcacgagc acgactcgag cagtcatcgg tggcgatcgt cctgtcatcg
     8941 ggccgacgtg tagagtcgca cgccgtacgg ctcgagccgc agcgcgattc gtgcgtccac
     9001 cggccggccc gtcaggcggt cgactgccac cgtcgcggcg acggccgccg gcagcacgag
     9061 tccgcggagt tcatcgcggc cgaggttggc gatgaaagtg agggtgcggc cctgcgcgga
     9121 gcgctgccag ccgatcacgt cgtcgcgacc gagatcgagc agcgtgaagt cgccctgcag
     9181 cagcgcgggc tcggcctgcc gcaggcggat ccagcggcgg taatgggcca gcagcgaggc
     9241 cggatcggct gactgcgtcg cgacgttgcg ccgttcgtgg ccgcgttgca gcggttccca
     9301 tggccgggcc gcccgcgtga agccgccgtt gggcccagac gtccacggca tgggattgcg
     9361 aatcatctcg tcgggcttgc gtccgacctg gccgatctcc tcgccgtagt acaggaatgg
     9421 cacgcccggc gccgcgagca gcaggctcgc cgcgagctcg aggcgcgcag gctcctcgcc
     9481 cagcacgctc gcgatgcggt cctggtcgtg gttggtgacg aaggtcgcga actgctgtcc
     9541 ggggtacgcg tcccaggcgt tctggagtgc atacgcgagg tcgtcgcgac gcccggactg
     9601 gatcgactgc aggattgcct tggcgacgtc gaactcgaac gcgaggtcga gcgctccacg
     9661 ctcgatgtac tcgctgacga cctcggtggc ggtccacacc tcgcccacca tcatcgcccc
     9721 gggcttcacg tgcttcacgt gcgtgcggaa cccgcgcaac cactcgatcg tctcaggcgt
     9781 gtcgctcatc acgtcaccgt cctcgatcag gtgacggatc gcgtcgagcc ggaacccgtc
     9841 gacgcccagg tcgcgcagcc agaactcggc gacgcggtac gcttccgccg tgactgcggg
     9901 attgcggtaa ttgaggtcgg gcatcccgga ccagaagatc ccgtagtagt gctggccgcg
     9961 ggcttcctgc cacacctgct ggtcccaggg accacgcacg tccggaacgg cgttcgcgtc
    10021 cacgaagatg tagcggtcgc ggaactgctg gtcgcccgcg agcgccttca cgaaccacgg
    10081 atggcgcgag gagctgtggt tcagcaccag gtcggtgatg accttgatgc cacggcggtg
    10141 acactcggcg atgaagcgct cgaagtcggc gcgcgtgccg tactggggat ggatgccgta
    10201 gtaatcggtg acgtcgtacc cgtgatagct cggcgacggg ctgatcggca tgagccagat
    10261 cccggtgatg ccgaggtcgg tggtcgtggc cgggtcgccg tcgttgaggt aatcgagctt
    10321 ctcgatcagt ccctggaagt cgccgatgcc gtcgtcggcg agcggcccgg tcgtcgagtc
    10381 ggcgaacgag cgcacgaaga tctcgtagaa gacgcgatcg ttccaccatc gcgcaccggc
    10441 cgccggcggt gcggccgcgg cagccggcgt cgccgacccg tcggatgcgg ccgccggcat
    10501 cgtggcgggt gtgccgcggc atcccgccag tgccagcagg cacagcactg gcagcaacgt
    10561 gcgtcgccgg ctcatgggtc gatcctcgat cggtgcgagc acggatcata gcggggaaac
    10621 agccgcgtcg tttcggcgcg cccgatccgg acgggtcggg cagacacgcc gtggcgaacc
    10681 ggtcggcgcc ggaaacgtga cggtcgtctc gtgcctcggc ccagccacat cgcctagcat
    10741 cggttccgtg cagcacgccg cggaattgca gttcgacgcc ggagtccgac gcgcggaaag
    10801 cgtctgcctg accgccgacg agttcgtgat cgaccgcagc ctgcgcggcg cgcgacgcgg
    10861 ccccacgctc acggtcgccc atcgccatcg ccgctgcgag tccgaactcg cgctgctcga
    10921 cggccaccgg ctggccgtgc gtgacaggcg tcgcgaggtg gtgcgccgct acgtggtcga
    10981 cctgcgtttc atagccgacg cgccggtcac gatccggacc gtcgcgtgga actgctggct
    11041 cgcgagcttc gcgagcctgg caacggccgg tctcgcggcg tggctcgcgt gggcgcagcg
    11101 tgcccatccg caggcgtggt cagcctgggt cgtggccctc gggctggcgg cgacggcgct
    11161 cctgctgggc gccgtcgccc tgtatcggac gtacgagacg acgctgctct tcagcgtgga
    11221 cggccacgtc acgctcgcca ggttgacggg cgcgctcggg tcgacgcgcc acgcggcggc
    11281 ctttcacgcc gagctgacac gcggcatcgc ggcggcccgt gccgcgatcg cgcaatcccg
    11341 gcaggcgttc ctgcgcgacg aactccgcga gcatcgccgc ctgtacgagg agggcgtgct
    11401 cccggcagac gcgtacgaag ccggcaagcg ccgcatcctg caggcgcacg actgatcggc
    11461 ggcagcgcct cgcaatgcgc agcgcatttg tcgtcgcctg acattccggc gcgcccgctc
    11521 cggacgggtc gggcagacac gccgtaagag cggtccctcg cggctcgaaa tgcggcaggc
    11581 agccctgagc ggccgcccgt gcggtcgctc gcacacgcaa acgccgggca gccgtcagcg
    11641 gccgcccgtg cggtcgctcg tgcgagtagg cgtcagcgca gcacctcgcc atcggcgtcg
    11701 agcacctgca cctcgccgag tccgagctcg cggatcggcg cctcgatgcg accgaggtcg
    11761 ccgacgatga cccacgtcag cgcgttggga cggaaggcgc gctgtgcggc ctgctggacg
    11821 cccgcgtccg tctgcgcctc gagccgctca cgcagcgtgc gaacccagtc gtcgggccgg
    11881 ccaaagcgga cgatgtcggc gatcgcgccg ctgaccgacg cgttcgtctc gtactgcccg
    11941 gacagcgcgc gcacgtcgcg ctggcggatc ttggcaattt cgtccgcggt cgccggactg
    12001 ggaccgacga actgctcgag ctcgcgccgg atctccgcta tggcctcgag cgtcttgtcc
    12061 gactgcaccg gcgccgacag cagccacggc cgctggccga gcgcttccgg caggctcgaa
    12121 cgcacgccgt aagaccagtg cttctgctcg cgcaggttca tgttcagccg cgacgtgaac
    12181 gtgccgccga cgaccgcgtt ggcggtgttg agctcgagtc ggtcctcggc ccggctcgac
    12241 ggcatcagct ggccgaccag cagcgtcgtc tgctgcgcgc caggccgatc gagcaggaac
    12301 acgcggggcg ccgcggcggg cggggacagc gggatcgcgg gccgtaccag ggaaacgccg
    12361 ggctcgggtc gccagtcacc gaactggcgt tcgagcagcg gcagcacctg ctccggcgtg
    12421 accgcgccag tgacgatcag cgtcacgttg tcgggtcgca gcacctgccg gtggaaatcg
    12481 acgaggtcct cgcgcgtgag cgccgcgacg gattcctccg taccggaccc cgagaacgga
    12541 atcgcatacg gatgtccgtc gccgtacatc accggtggca gcacgcgcag cgccagcgcg
    12601 tcagggctcg tcttttcgcg cgcgatgccg gcgatccact ccttgcggat acgctcgatc
    12661 tcggccgtcg ggaaggtggg ccggcgcacc acgtccgcga gcaactgcag cgacggctcg
    12721 agcttctcgg tcagcgtcga caccgcgacg aacgacgtgt cgagtgccga accggccgag
    12781 aggtccgcgc cgaggcgctc gacgcggtcg gcgaggccga gtgcgtcgaa ctggccggcc
    12841 ccttcgtcga gcatgcccat tgcgaagctc gacgtgcccg gcttgcggcc gagatcggcc
    12901 acgtagccgg cgtcgaagag cagcgacact cgtgccaccg gcacgcccgg gcgcgtggcg
    12961 acgatcacgg ggatgccatt cgacagcttg cggcgctgca ggtcggggaa ggcgacgtcc
    13021 ggaaagctcg tgatcgtcgg cgggcctttg cttcgatcga ccgccgaggt cgcagccgcc
    13081 ttgtaaggag gctgcggcag cactgtgagc gtgtagtcgc cctgcatcag ccagcggcgc
    13141 gcgacctgct gcaggtcggc cggcgtcgcg gccgcgatcg tccgcagcga gcgccggtag
    13201 cagcccgggt cgccttcgta gacctcgcag gaagcgagca cgtcggcctt gccgccaaag
    13261 ccgccgatgc gttcgagtcc gcgtatgaac gaggaacgga atacggtctt ggcgcggtcg
    13321 agctcggccg ccgcaggacc ctgctcgatg aagcggcgca gttcctcttc gacggcagct
    13381 tcgacgcgtt cgacggggac acccgtcttg acgtccgcgg agatgctgaa caggcccgcg
    13441 atctcgagcg gcgactgccc ggcgccgacc gtgtcggcga gccgctcgcg atagacgagc
    13501 cgttcgtaca gtcgcgaggt cttgccgccg cccaggatct cggcggccag cgcgaggtgc
    13561 tcggcgtcgg cggagccgtc gggcggggtg ttccagtagc gctgccaccg cggctgcgca
    13621 acctgatcgt acatggtctc ccgcttgctt tcggtgcgcg ccgcgatcca ctcgccgggg
    13681 cgtgtgaggg ccggtcccgg cgcgatgtgg ccgaagtact gctgtgcctt ggcgcgtgcc
    13741 gtggcgacgt cgatgtcgcc ggccagcacg agcacggcgt tcgcggcgcc gtagtacgtc
    13801 ttgaaccagt cgcgaacgtc gtcgagcgtc gccgcgtcga ggtcctccat cgagccgatc
    13861 ggcagcgtgc gatacgggtg acccggtggg aagcttgcac ggaacacggt ctccggcacg
    13921 agaccgaacg gccgattctc gccctggcgc ttctcgttct gcacgacgcc gcgctgctcg
    13981 tcgagcctgg cctggtcgac cacgccgagc aggtggccca tgcgatcgga ttcgagccac
    14041 agcgccaggt cgagcgccgt ggtcggcacg ttctggaaat agttggtccg atcgaagttc
    14101 gtggtgccgt tcatgctcgt ggcgccgacg agttccagcg ggcggaagaa ctcgtcgttg
    14161 aagtgctcac tgccctggaa catcaggtgt tcgaacaggt gcgcgaaccc ggtgcgaccg
    14221 ggagtctcgt tcttggagcc cacgtggtac cagaggttga cggcgacgat cggcgccttg
    14281 cggtccgtgt ggacgatcac cgtcagcccg ttgtcgagct ggaacctgtc gaacgggatc
    14341 agctggtcta cgggcgcggc aaccgctgcg gccgcgacgg ccggcgccga cgggatcgtc
    14401 accagcgcca gccaggcggc ggagaccgcc agccgatgta agcgaatccc tacgctcgtg
    14461 ccgcgcacca aaacagcccg cttgttgctt agggacgtca aaaagcctcc tccgaatcca
    14521 cttcggcgcc tcgcacgaac cacggagccc tgcgccgctg cggtaatacc ggatttaccg
    14581 cactggactt tgcctgacca taacgcggaa aattgggttc ggtggaccgt tactgctcgt
    14641 tcggggtggg actggcgagc tgatggtgag cgatcaccgt cgacccgata atgcatggca
    14701 taaaagccac aaggagaacg cctcgatgtc atcccgttac ctcatttccc tggctgtagc
    14761 ggcagccctt gcagcgccgg ttgcggccca ggccgaacaa ggcgactggc tgttccgggt
    14821 cggcttgtcg gtgaccaatc ccgactcgac caacctcgaa ctgccgccgg tcggcgacat
    14881 tgtcatcgat gacgatatct caccgacgtt caacatcagc tacttcctca cggaccacat
    14941 cagcaccgag ctgctggcgg catggccgtt cacgcacggc gtcgatctca agacgggtgg
    15001 cggcggagac gacaagaccc gtatcggcaa cgtcgatcac ctgccgccga cgctgagcct
    15061 gaactggcac ttcaatccgg acggcacctt ccgcccgtac atcggcgtcg gtgcgaacta
    15121 cacgctcttc agcagtgagg agacgcgtgg cccgctcacc ggttcgatcc tgaagctcga
    15181 cgactcgttc ggcgcggccg gccaggtcgg tgtggacatc ggttccggca actggttctt
    15241 caacgcgaac gcgcgataca tccagatcga gtccgacgcg acactcgacg gtgccgacat
    15301 cggcacgatc gacgtcaacc catgggtgta cggcgtgcac atcggctaca agttcggtcg
    15361 cccggctccg gtggcagaac ccgagccggc gccggccgcc gcgccgccgc ctccgccccc
    15421 gccgcctccg ccgccgcccc ccgccgatgc cgacggcgac ggcgtgacgg acgacctcga
    15481 caagtgcccg ggcactccgg ccggcaccaa ggtcgacaag gtcggctgcc cgctggagca
    15541 gacgctcaag ctgctgttcg acttcgacag cgccgagctt cgccccgagt cgatcaccga
    15601 gctcgagcgc gtcgtgaagt tcatgaacga cgtgccgttc gcgatggtgc tcgtccaggg
    15661 ccacacggac agcgtcggtg gtgacgcgta caacctggcg ctgtcggatc gtcgcgccaa
    15721 ggccgtgttc gactacctct cgagccgcgg tgtcgacccg gcgcgcgtga agtcggaagg
    15781 caagggcgag gccgagccga tcgccgacaa caagacggct gaaggccgcc aggagaatcg
    15841 tcgcgtcatg ctgatccgca cggattcggg catgtaagcg gctgcacagc caccctgcgg
    15901 aggctgtcga cgaggccccg acgcgcaagc gcggggcctt cgtcttttcg ggccacgcac
    15961 ttttcgggcc gtgcgcgacg agccgtgcac cctggtcggt caaacgacgg caccggacgg
    16021 tagaatggcc gtttcgtgcc tgcgcgaaag gagtccatcg catgaagccc gtccacgcac
    16081 ttgccctgat gacggccctg gccgtcccgc ccgccttcgg cgccgacacc gcaacggaca
    16141 ccggcgcggc aggcgccgac gccgcgctgc aggccgccgt gaacggcgac tggcgcagcg
    16201 ccgatgaaaa gtcgcgcgac cgctaccgtc acccggtcga gtcgctcacg ttctggggat
    16261 tgaagcccgg catgaccgtg ctggaagtgc agcccggcgg cggttggtgg acgaacatcc
    16321 tcgcacccta tgccagggcg accggcggca agctctacgt gacggccgcg gacctccaga
    16381 acccgaagct ctccgacgcc gcgaaaaagg cgcgcgccga cttcgaggcg cgttacgccg
    16441 cgcggcccga aacgttcggc aagctcgatt acgtcgactg gggagccgtc tcgaggtcgt
    16501 tgccggcgaa cacgttcgac ctgatcctga cggcgcgctc ggtccacggc tggatgcagc
    16561 aggggccgga cgtggcgcag cagacgttcg ccgagttcca cgaggcgctc aagcccggcg
    16621 gcatcctggc cgtcgagcag catcgggcgc acccgaagaa cggggccgcc gaggacccga
    16681 agtccgggta cgtgtccgag cagcacgtga tcgacctggc gcagaaagcc ggcttcaagc
    16741 tcgccgcaaa gtcggagatc aacgcgaacc cgaaggacac caaggatcat cccttcggcg
    16801 tgtggacgct gccgccggcg ctgtactcga caccgtacgg ttcgggccag ccggacgacc
    16861 cgaagttcga ccacagcaag tacctcgcga tcggcgaatc cgatcgcatg acgctcaagt
    16921 tcgtgaagtg acgggtgggg cacactgatg cggaagagtc cgtcccccgc gatcacgacc
    16981 gcgttgctgc tgtcgacggc gctcgccggc tgcgccagcc agcccgcgcc gtccgacgtc
    17041 ccgcaggcct ccacgccggc cgccaccgcg cctgcggtgg cgacgtcgga gtcgcagaag
    17101 ctgcaggcga tattcgaaga atatttcgag gagacc
//
LOCUS       DASPFR010000200         5498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_423103, whole genome shotgun sequence.
ACCESSION   DASPFR010000200 DASPFR010000000
VERSION     DASPFR010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423103"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1409
                     /gene="xseA"
                     /locus_tag="VD737_05140"
     CDS             <1..1409
                     /gene="xseA"
                     /locus_tag="VD737_05140"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HXW09978.1"
                     /translation="APMTDSLFDDAPPAALPPREVLTISQLNAQARVLLERGLGSVWL
                     EGEISNLSRPASGHWYFSLKDASAQVRCAMFRTRAMLVRFPLRDGARVLARGRVSLYE
                     ARGEFQVVVEHLEEAGEGALRRRFEDLKRKLAAEGLFESSRKRRPPVLPRRVGIVTSR
                     TGAALRDVLHILRRRFPAVPVLIYPCAVQGDSAAREIVEALRVAAQRAECDVLIVARG
                     GGSLEDLMAFNDEAVARAIAASPIPVICGVGHETDFTIADFVADERAPTPSGAAERAV
                     PDASEFLRALGALERNLAAAMLRKLRTLGQALHLQQRGLARTHPGARLRQHAQRLDEL
                     DQRLQRAVSRRLARSHDRLERARALLGRSSPARRLATLRLRLERAARQLPLCMQRRVQ
                     RAHDRYGRATRTLHAVSPLATLDRGYAIVLDAAGRVVSDAASLEPGAAIEARLARGTL
                     HATVASVIGAPAAPASER"
     gene            1406..2323
                     /locus_tag="VD737_05145"
     CDS             1406..2323
                     /locus_tag="VD737_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXW09979.1"
                     /translation="MTGRRGRRTGRAVAIASQTMAVVACLAVPADGTFGQDAVAPGDA
                     VRAELADVLPIDTRVPGGVAIIRLGDETLPPGEMYYGPYRAPVVHDGRSWVAVIGIPL
                     ATLPGAQTAVLVTPDPDDTDLEIEFEVGPKTYAEQRLTVEPRKVDPLPQDLVRIEAET
                     ERSDRALSTYSEEFWPTWRWAAPVPGPRSSSFGLRRVFNGQPRSPHSGMDIAAPTGTP
                     IRAPAEGRVLDVGDFFFNGNTVFVDHGRGVVTMYCHLSRIDVQPGQAVAAGTTLGLVG
                     ATGRVTGPHLHFGVAVNRAMVDPALFLRE"
     gene            complement(2401..3546)
                     /locus_tag="VD737_05150"
     CDS             complement(2401..3546)
                     /locus_tag="VD737_05150"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HXW09980.1"
                     /translation="MAVARTSPSAAHDLPATRLAILDEPLRLYRGGVVEHPVIAYETW
                     GRLNDARDNTVLIFTGLSPSAHAASSPADPKPGWWEPMIGDGKAIDTSRFFVVCVNSL
                     GSPFGSSGPASIDPRTGRPYGLDFPEVAVEDIARAGYEVLRQLDIWTVQCVIGPSLGG
                     VTVLAFSVQFPEVTRNLISISGAASASPFAIALRSLQREMVRSDPAWRGGHYEPGRGP
                     RQGLRLARKLGTITYRGHEEWDQRFGRRRVAETAGLQGDFRPQFEVEAYLEHQGQRFA
                     EVFDANAYLYLSRASDQFDLAEHGGGSLEKAYAKFKVERTLVLGVQSDMLYTIDQQQA
                     IAEHLRAAGREVEFHAFPSPQGHDSFLVDYARFEPAIGNFLKKVASG"
     gene            complement(3649..5073)
                     /gene="der"
                     /locus_tag="VD737_05155"
     CDS             complement(3649..5073)
                     /gene="der"
                     /locus_tag="VD737_05155"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020752350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HXW09981.1"
                     /translation="MLPVVALVGRPNVGKSTLFNALTRTRDALVADVPGLTRDRKYGY
                     ARQQGRSCVVIDTGGLVETVQGGIERLMAEQTLKAVEEADRVLFVVDAREGTTPQDLH
                     VADLLRRSGRPVLVVANKSEGLEDEMAQADFHAFGFGEPVAVSAAHLQNLDLLFDRAL
                     EGLAGADEVPEPLSGTVERQAIRVAVVGRPNVGKSTLINRLLGEERLIAFDQPGTTRD
                     TVEVPFERDGQSFVLIDTAGVRRRARVEQAIEKFSIVKTLQAIDRAHVVIGVLDAHDT
                     VAEQDASLLGLVAEQGRALVIAVNKWDGIPADQRNAIRDGLDLRLDFLRYASVHFISA
                     RHGTGVGDLVGSVISAYRAAMREMPTKDLTRALEQAITIHQPPLVRGRRIKLRYAHQG
                     GRNPPVVVIHGNQTQHVPDAYRRFLENHFRQVFRLHGTPLRVEFRTDDNPFAGRRNEL
                     TPRQVRSRKRLMARVKGSRKGGKR"
     gene            complement(5075..>5498)
                     /locus_tag="VD737_05160"
     CDS             complement(5075..>5498)
                     /locus_tag="VD737_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXW09982.1"
                     /translation="YAVGYQGRVVMFALDSGQVWWGRELSSYRGLALDADQLYVATSD
                     GSVVALRRRDGTVLWQQDGLKRRGLGAPAVVGNAVVVGDFEGYLHWLDRDTGKFIARE
                     RPGRTRISAAPVVDGNRVYVIDEGGRIAAYSSGGPPER"
BASE COUNT          791 a         1925 c         1974 g          808 t
ORIGIN      
        1 ctgcaccgat gaccgactcc ctgttcgacg acgcgccgcc cgccgccctc ccgccgcgcg
       61 aagtgctcac catctcgcag ttgaacgcgc aggcgcgcgt gctgctcgag cgcgggctcg
      121 gctccgtctg gctcgagggc gagatctcca acctgtcgcg ccccgcctcc ggccactggt
      181 acttctcgct caaggacgcg agcgcgcagg tccgctgcgc gatgttcagg acgcgcgcca
      241 tgctcgtgcg cttcccgctg cgcgacggcg cgcgcgtgct cgcccgcggc cgcgtgagcc
      301 tgtacgaggc ccgtggcgag ttccaggtcg tggtcgaaca cctcgaagaa gcgggcgaag
      361 gcgccctgcg acgtcgtttc gaggacttga agcgcaagct cgcggccgag ggtctcttcg
      421 agtcgtcgcg aaagcgcagg ccgcccgtcc tgccgcggcg cgtcggtatc gtcacctcgc
      481 gcaccggtgc ggcgttgcgt gacgtcctgc acatcctgcg gcgccgcttc ccggccgtgc
      541 cggtcctgat ctacccgtgc gcggtccagg gcgattctgc ggcgcgggaa atcgtcgagg
      601 cgctgcgcgt cgccgcgcag cgggccgaat gcgacgtgct gatcgtggcc cgcggcggtg
      661 gctcgctcga agacctgatg gcgttcaacg acgaggccgt cgctcgcgcg atcgccgcgt
      721 cgccgattcc cgtgatctgc ggcgtgggcc acgagaccga tttcacgatc gccgacttcg
      781 tcgcggacga gcgcgcgccc acgccctccg gggcggccga gcgtgccgtg ccggacgcct
      841 ccgagttcct gcgcgcactc ggggcgctcg agcgcaacct ggccgcagcg atgctccgca
      901 agctgcgcac gctgggacag gcgctgcacc tgcagcaacg tggccttgcc cgcacgcatc
      961 ccggcgcaag gttgcggcag cacgcgcagc ggctcgacga actcgaccag cgcctgcagc
     1021 gcgcggtctc gcgccggctc gcccggtcac acgaccgcct cgagcgagcg cgcgcactgc
     1081 tcgggcgcag cagccccgcg cggcggctgg cgacgctgcg cctgcggctc gagcgtgccg
     1141 ccaggcagtt gccgctctgc atgcagcggc gcgtgcagcg ggcgcatgac cgctacgggc
     1201 gcgcgacgcg cacgctgcac gcggtgagcc cgctcgcgac gctggaccgt ggctacgcga
     1261 tcgtgctcga cgcagccgga cgcgtggtca gtgatgcggc gagcctcgag ccaggagccg
     1321 cgatcgaggc tcggctcgcg cgcgggacac tgcacgcgac cgtcgcgtcc gtgatcgggg
     1381 caccggcggc accggcctcc gagcgatgac cggccggcgc ggacgtcgta ccggtcgcgc
     1441 cgtcgcgatc gcctcgcaga cgatggcggt cgtcgcgtgc ctcgccgtcc cggccgacgg
     1501 cacgttcggg caggacgccg ttgcgccagg cgacgccgtt cgcgccgaat tggcggatgt
     1561 cctgccgatc gacacccgcg tcccgggtgg agtggcgatc atccggctgg gcgacgaaac
     1621 cctgccaccg ggcgagatgt attacggccc gtaccgggcg ccggtcgtgc acgacggtcg
     1681 ctcgtgggtc gccgtgatcg gcatcccgct cgcaaccctg cccggggcgc agacggcggt
     1741 gctggtgacg ccggacccgg acgacacaga cctcgagatc gagttcgaag tgggtccgaa
     1801 gacctatgcc gagcagcgtc tgaccgtcga gccgcgcaag gtcgatccgc tgccgcagga
     1861 cctggtgcgg atcgaggcgg aaaccgaacg gtcggatcgg gcgttgtcga cctattcgga
     1921 ggagttctgg ccgacctggc gttgggcagc gccggtgccg ggaccgcgct cgagttcgtt
     1981 cggacttcgg cgcgtgttca acggccagcc ccggagcccc cacagcggca tggacatcgc
     2041 tgccccgacg ggcacgccca tccgcgcgcc ggcggagggt cgcgtgctcg acgtcgggga
     2101 ttttttcttc aacggcaaca ccgtgttcgt cgaccacggg cgcggggtcg tgacgatgta
     2161 ctgccacctg agccgcatcg acgtgcagcc gggccaggcc gtggccgccg gcaccacgct
     2221 cgggctcgtc ggcgcgaccg gtcgcgtgac cggaccccac ctgcacttcg gggtggcggt
     2281 gaaccgggcg atggtggatc cggcgttatt ccttcgggaa taacgccgga agtggctggt
     2341 ggctagtggt cagtggctag tggtgggaaa caagagcgcg cggcgtgctc ttactagcca
     2401 ctagccacta gccactttct tcaggaaatt gccgatcgcg ggctcgaacc gcgcgtagtc
     2461 gaccaggaac gagtcgtggc cctgcggcga ggggaacgcg tggaactcga cctcgcgtcc
     2521 cgcggcgcgc aggtgctcgg cgatcgcctg ctgctggtcg atggtgtaga gcatgtcgga
     2581 ctgcacgccg agcaccagcg tacgctcgac cttgaacttc gcatacgcct tctcgagcga
     2641 gccgccgccg tgctcggcca ggtcgaactg gtcggaggcc cgcgagagat acaggtacgc
     2701 gttggcgtcg aacacctcgg cgaagcgttg tccctggtgc tcgagatacg cctcgacctc
     2761 gaactgcggg cggaaatcgc cctgcagacc ggccgtctcc gcgacgcggc ggcgaccgaa
     2821 gcgctggtcc cactcctcgt gaccgcgata ggtgatcgtg ccgagcttgc gcgcgagccg
     2881 cagtccctgg cgcggcccgc gaccgggttc gtagtggccg ccgcgccagg cgggatcgct
     2941 gcgcaccatt tcacgctgca gcgagcgcag cgcgatcgca aacggcgagg cgctcgcggc
     3001 gcccgaaatc gagatcaggt tgcgcgtgac ttccggaaac tgcaccgaga aggccagtac
     3061 cgtgacgccg cccagcgacg gcccgatgac gcactggacg gtccagatgt cgagctgccg
     3121 caacacctcg tagccggcgc gcgcgatgtc ctcgaccgcg acctccggga agtccaggcc
     3181 atacggccgg ccggtgcggg gatcgatcga cgcgggcccg gacgacccga acgggctgcc
     3241 gagcgaattg acgcagacga cgaagaaccg gctcgtgtcg atggccttgc catcgccgat
     3301 catcggttcc caccagccgg gcttcgggtc ggcgggcgac gaagccgcgt gggcggacgg
     3361 cgaaaggccc gtgaagatca ggacggtgtt gtcacgggcg tcgttgagcc ggccccaggt
     3421 ttcgtacgcg atgaccggat gctcgaccac accgccgcga tacaggcgca gcggttcgtc
     3481 caggatcgcc agccgcgtgg cgggcaggtc gtgcgcggcg gacggggagg ttctggcgac
     3541 ggccatcagc atgccctgca gtctagacgc ggctgccgtg cgggcaagcc tcagacgata
     3601 gccggtcaga accggaggtt atgagcgtac cgagccggcg ccgcgctgtc agcgcttgcc
     3661 ccccttccgg ctgcccttga cccgcgccat caggcgcttg cggctgcgga cctggcgtgg
     3721 cgtgagttcg ttgcgccggc ccgcgaacgg attatcgtcc gtgcggaact ccacccgcag
     3781 cggcgtgccg tgcagccgga acacctggcg gaagtggttc tcgaggaagc gccggtacgc
     3841 gtccggcacg tgctgggtct ggttgccgtg gatcacgacg acgggcggat tgcggccacc
     3901 ctggtgcgcg tagcgcagct tgatgcgccg accgcgcacg agcggcggct ggtggatggt
     3961 gatcgcctgt tcgagggcgc gcgtgaggtc cttggtcggc atttcgcgca tggcggcgcg
     4021 gtacgccgag atcaccgagc cgaccaggtc gccgacgccg gtgccgtgac gggccgagat
     4081 gaagtgcaca gacgcatagc gcaggaagtc caggcgcagg tcgagcccgt cacggatcgc
     4141 gttgcgctgg tcggcgggga tgccgtccca cttgttgacc gcgatcacga gcgcccgccc
     4201 ctgctccgcc acgagcccga gcaggctcgc gtcctgttcg gcgaccgtgt cgtgggcgtc
     4261 gagcacgccg atcacgacgt gtgcccggtc gatcgcctgc aaggtcttga cgatgctgaa
     4321 tttctcgatc gcctgctcga cgcgggcgcg gcgccggacg ccggccgtgt cgatcagcac
     4381 gaacgactgg ccgtcgcgtt cgaacggcac ctcgacggtg tcccgggtcg tgccgggctg
     4441 gtcgaaggcg atcagccgct cctcaccgag caggcggttg atcagcgtcg acttgccgac
     4501 gttgggacgg ccgacgaccg cgacgcggat cgcctgacgc tcgaccgtgc cgctgagcgg
     4561 ttccggcacc tcgtcggcgc cggcgaggcc ttcgagcgcg cgatcgaaca gcaggtccag
     4621 gttctgcagg tgcgccgcgg agaccgcgac cggctcgccg aagccgaacg cgtggaagtc
     4681 ggcctgcgcc atctcgtcct cgaggccctc ggacttgttg gcgacgacga gcacgggccg
     4741 accggaccgc cgcagcaggt cggcgacgtg caggtcctgc ggcgtggtgc cctcgcgcgc
     4801 gtcgacgacg aagagcacgc gatccgcctc ctcgacggcc ttcagggtct gttcggccat
     4861 caggcgctcg atgccgccct ggacggtctc gacgaggccg ccggtgtcga tcaccacgca
     4921 cgagcgtccc tgctgccgcg cgtaaccgta cttgcggtcg cgcgtgaggc cgggcacgtc
     4981 cgcgacgagc gcgtcgcgcg tccgcgtcag cgcgttgaag agcgtggact tcccgacgtt
     5041 cgggcggccg acgagggcga cgacgggcag cacatcagcg ctccggcggg ccgccgctgc
     5101 tgtaagcggc gatgcggccg ccttcgtcga tcacgtacac gcgattgccg tcgaccaccg
     5161 gcgccgcgct gatccgcgtc cgaccgggac gctcgcgcgc gatgaacttg ccggtgtcac
     5221 ggtcgagcca gtgcaggtag ccctcgaagt cgccgaccac cacggcgttg ccgaccacgg
     5281 cgggggcgcc gagaccgcga cgcttcagcc cgtcctgctg ccacagcacg gtgccgtccc
     5341 gacggcggag cgcgaccacg ctgccgtccg aggtggccac gtagagctgg tcggcatcga
     5401 gcgcgaggcc gcggtagctc gacaactcgc ggccccacca tacctggccg gagtcgagag
     5461 cgaacatcac gacgcggccc tggtaaccga ccgcgtag
//
LOCUS       DASPFR010000201         2309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_57543, whole genome shotgun sequence.
ACCESSION   DASPFR010000201 DASPFR010000000
VERSION     DASPFR010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57543"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VD737_05165"
     CDS             complement(<1..894)
                     /locus_tag="VD737_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HXW09983.1"
                     /translation="MAWNRKRIVVAALAGAFVAVVVTVVALNFVTSESDIETKLEHRY
                     NVDDPQFRREIGILLGPPIIDGNRIENFENGVEIFPAMLAAIRGARSTITFETYIYWS
                     GSVGRQFADALGERARNGVRVHLLIDWVGSQNMEQPLLDEMKSAGVEVHRYHPLRWYN
                     LTRMNNRTHRKLLVVDGRVGFTGGVGISDEWDGDAHDPEHWRDSHYRLEGPAVAQMQA
                     AFMNNWIKTTGEVLQGKDYFPALEPAGTALGQVFTSSPSGGGDSMQLMYLMSITAAQQ
                     RIDLSAAYFVPDPLTRRALRQA"
     gene            complement(1095..>2309)
                     /locus_tag="VD737_05170"
     CDS             complement(1095..>2309)
                     /locus_tag="VD737_05170"
                     /inference="COORDINATES: protein motif:HMM:NF025068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HXW09984.1"
                     /translation="GVAEIDGQTYELRPDAADGRHLLAEVDTSRLPPLEPEMAVGPVR
                     EKPDPSQFRALGHLGADLAPVVHDVLVLYTAASARRYGTSTLQSMIVGGVAAANEAYR
                     ASGVGITLQLAGLQQAVTIAETRGGMVDTLWSLARSAEASDLRDRHAADIVLLVNESD
                     DWCGYAYIMHDPNPAYASQAYGAVQARCLSSEAIAHEVGHIQGLVHDRDTRDEDGALP
                     YGHGYRLCQRGGFRDVMSYQCPNVDVPRVRLFSNPARSYRGVPTGIAHETDPGRSADA
                     ARALNETAPLVAAYRIAEAAVDAPRPPVGLTASVRRGVARLDWRARDPQGLGFAVDRS
                     SGGDFTEIARVDSDVTRFVDTTVARPATYSYRVRSLATDGASSTSEVVTVTIRDRHGR
                     PVRDVGRRELPR"
BASE COUNT          346 a          787 c          821 g          355 t
ORIGIN      
        1 cgcctggcgc agcgcgcgcc gcgtgagcgg atcgggcacg aaataggccg cggacaggtc
       61 gatgcgctgc tgggccgcgg ttatcgacat caggtacatc agctgcatgc tgtcgccgcc
      121 gccggacggc gaactcgtga acacctgccc gagcgcggtg ccggcgggct cgagcgccgg
      181 gaaatagtcc ttgccctgca gcacttcgcc ggtggtcttg atccagttgt tcatgaaggc
      241 ggcctgcatc tgcgcgacgg ccggcccctc gagccggtag tgcgagtcgc gccagtgctc
      301 gggatcgtgg gcgtcgccgt cccactcgtc gctgatgccc acgccgcccg tgaaaccgac
      361 ccggccgtcg accacgagca gcttgcggtg cgtgcggttg ttcatgcgcg tcaggttgta
      421 ccagcgcagc gggtggtacc ggtgcacctc gacgcccgcc gacttcatct cgtcgagcag
      481 tggctgctcc atgttctggc tgccgaccca gtcgatcagc aggtgcacgc gcacgccgtt
      541 gcgggcgcgc tcgccgagcg catcggcgaa ctggcggccc acgctgccgg accagtagat
      601 gtaggtttcg aacgtgatgg tcgagcgcgc gccgcgaatc gccgcgagca tggccgggaa
      661 gatctcgacg ccgttctcga agttctcgat acggttgcca tcgatgatgg gcgggccgag
      721 caggatgccg atctcgcgcc ggaactgcgg gtcgtccacg ttgtagcggt gctcgagctt
      781 tgtctcgatg tcgctctcgg acgtgacgaa attgagcgcg acgacggtga cgacgacggc
      841 gacgaacgcg cccgccaggg ctgcgacgac gatgcgcttg cgattccagg ccatggacgt
      901 cctttcggca gtgtgccgaa gccgagcgtt gcgggcggaa cggacgctga ctatcagacg
      961 gtcacccaaa gtcgcctcgc gacggcggca cgcagtgacc gcgtatcgcc aaagcgcagc
     1021 ctgtcacggg tggagcgttt cgtggccgcg gcggcgccac tgcggactac cgacacttcc
     1081 gcggcatcaa aaggtcagcg aggcaactcg cggcggccga cgtcgcgtac agggcggccg
     1141 tggcggtcgc ggatcgtgac ggtgacgacc tcggacgtgc tggacgcgcc gtcggtggcg
     1201 agcgagcgca cgcggtacga atacgtggca gggcgcgcca cggtcgtgtc gacgaacctg
     1261 gtgacgtccg aatcgacgcg cgcgatctcg gtgaaatcgc cgccgctgct gcggtcgacc
     1321 gcgaatccga ggccctgggg atcgcgcgcg cgccagtcga ggcgcgccac gccgcgacgc
     1381 acggacgcgg tcaagccgac aggcgggcgc ggcgcatcga cggcggcctc ggcgatgcgg
     1441 taggccgcga cgaggggcgc cgtttcgttg agtgctcgcg cggcgtcggc ggagcgccca
     1501 ggatccgttt cgtgcgcgat gccggtcggc acgccccggt acgagcgcgc cggattggag
     1561 aacagccgca cgcgcggcac gtcgacgttc gggcactggt aggacatcac gtcgcggaag
     1621 ccgccgcgct ggcacaggcg gtacccgtgg ccgtacggca gcgcgccgtc ctcgtcgcgc
     1681 gtgtcgcggt cgtgcaccag gccctggatg tgtcccacct cgtgcgcgat cgcttccgac
     1741 gacaggcacc gcgcctgcac cgccccgtac gcctgcgagg cataggccgg attcgggtcg
     1801 tgcatgatgt acgcgtagcc gcaccagtcg tcgctctcgt tcacgagcag cacgatgtcg
     1861 gcggcgtggc gatcgcgcag gtccgatgct tccgctgagc gcgcaagcga ccacagcgtg
     1921 tccaccatgc cgccacgtgt ttccgcgatc gtgacggcct gctgcaatcc ggccagctgc
     1981 aacgtgatgc cgacgccgct cgcccgatat gcctcgttcg ccgcggccac gccgcccacg
     2041 atcatgctct gcagcgtcga ggtgccgtag cgccgagcgg atgcggccgt gtagagcacc
     2101 agcacgtcgt ggacgacggg cgcgaggtcc gcgccgaggt gaccgagtgc gcggaactgc
     2161 gacggatcgg gcttttcccg cacgggcccg acggccattt ccggctcgag cggcggcagc
     2221 cgtgacgtgt ccacctccgc cagcaaatga cgaccgtcgg ccgcgtccgg ccgcagctca
     2281 tacgtctgcc cgtcgatctc ggccacgcc
//
LOCUS       DASPFR010000202         6031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1522835, whole genome shotgun sequence.
ACCESSION   DASPFR010000202 DASPFR010000000
VERSION     DASPFR010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6031
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522835"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..481)
                     /locus_tag="VD737_05175"
     CDS             complement(266..481)
                     /locus_tag="VD737_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HXW09985.1"
                     /translation="MTAADRSEKLIQPNAQHQYDVTAADLPLSCPMPGMHLWNSHPRV
                     FLAVEKTGWAKCPYCSAEYTLRKVVGG"
     gene            complement(549..1649)
                     /locus_tag="VD737_05180"
     CDS             complement(549..1649)
                     /locus_tag="VD737_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXW09986.1"
                     /translation="MQPLFTSPPESVCILRLSALGDVCHVLPVVRTLQDAWPQTRLTW
                     ILGKVEHKLLGHVPGVEFVVFDKKAGAAGYRALRERLASRRFDALLHMQLALRASLAA
                     ALVPARWKVGFDRGRAREGQWLFTNVQIEPRTREHVLDSLFGFAERLGLGTRSMRWDI
                     PLPDTALEYAGRAIPDTSRPTLVLSPCSSHASRNWSAERYAALADHAVVRHGMEVLLC
                     GGRSDLELRYGRDIERCMRQPSRNLIGQDTLLEFLATLQRATALVSPDSGPAHMATTV
                     GTPVVGLYAATNPQRSGPYYSRQWCVDRYERAATRYLNRSASRVPWTEKIERPGVMDL
                     IEVDDAVEKLDELMAAGAPRTGPTRPAEPILR"
     gene            1701..2435
                     /locus_tag="VD737_05185"
     CDS             1701..2435
                     /locus_tag="VD737_05185"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: protein motif:HMM:NF002475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HXW09987.1"
                     /translation="MNPAAVAGDEALARIAGGAMLYDAARVGQPEAAIFEPAYWQARG
                     AAVGTRGGRGTVTFVRGADGEPWVLRHYRRGGAVARLSSDAYLWTGAPRTRSFAEWRL
                     LRQLRDWNLPVPRPIAARYLRRGLTYRADLITEELPTRRTLAASLADAPLPTAAWHAI
                     GRCIARLHARGVHHADLNAHNLLLGDDGAVYVLDFDRGRIRRRGSWERVVLARLRRSL
                     DKVTAKYPPGRFGHEQWRALLTGVEE"
     gene            2443..3747
                     /gene="waaA"
                     /locus_tag="VD737_05190"
     CDS             2443..3747
                     /gene="waaA"
                     /locus_tag="VD737_05190"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF004388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HXW09988.1"
                     /translation="MMRLLYVALTYLLAPVFIVREAWQAWRDRSQRGRVAQRLGYVAR
                     APRDADVWVHAVSVGEVQAAAALVRALRRRRPDLAIALTTTTATGAQRAHALFGDTVH
                     HGYLPYDLPGPVRRFLDRVSPRVAVILETEIWPTLYATLAHRRIPLVLASARLSARSV
                     DRYRRFAALFGESLAQHVAIGAQTAEDAERFRRIGAPAERVQVTGNVKYDLEIPAGQV
                     EAGRALRERWGRERPVWIAGSTHEGEEAAALAAHEAVLERFPDALLLLVPRHPPRFEA
                     VRALLQRRGVPFARRGAGAVPAPTQTVYLVDTLGELQAFYAASDVAFVGGSLVPIGGH
                     SLLEPAVLGVPILSGPYTQNGREIAELLERAGALELVADAPALGRALLAWFEAPSVAR
                     DAGESGRRAVAANRGAVDRLVGMILPRLVRSSDAPPAASSVP"
     gene            complement(3704..5140)
                     /locus_tag="VD737_05195"
     CDS             complement(3704..5140)
                     /locus_tag="VD737_05195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HXW09989.1"
                     /translation="MTRKSLLLACSVAAVLVAGMPVRAENLIDVYQDALSSDPVLREA
                     EARRMAALEAKPQARGLLLPQVDVSGQYATRDSESESTFTQSVDHDDNEATPNIIVQV
                     PNNQTVDSDFWSYDAQITQSLFRWDQWQALKRADAQVAVAEANFRAAQQNLLVRVSTA
                     YFDVLAAEDTLAAAEATLEALTRQLEQADKRFEVGLIAITDVQEARAAHDSATAGVIA
                     AKRTLATTQEALRELTNEAYTTLAKPAEQMPLDAPNPADEQAWVDRALENNISVIAAR
                     LETEVAARDVKVARAGHYPAIDLFARRSEFDGDTTATNNGLTGPADSTQTDDTIGVQV
                     TIPIFSGGVTQSRVRESVYLQRAARERLEGSMRSAERETRDAYLGVIAEKARVQALAQ
                     AVKSSQTALEATEAGFEVGTRTTVDVLAARRTLFEAQRDYARSRYDYLINIVRLESAA
                     GGLVQDDLARINSFLTAPTTLPVVRPKT"
     gene            complement(5279..5932)
                     /locus_tag="VD737_05200"
     CDS             complement(5279..5932)
                     /locus_tag="VD737_05200"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HXW09990.1"
                     /translation="MFDVQSARRQMIEQQVRAWEVLDLRVLEALERVPREEFVPAAYR
                     ELAFADTSVPIGHGQAMLPPKVEGRILQALDLQPGDHVLEVGTGTGFFAACIGQLARS
                     VRTIDIHAEFVPKAEATWRRVGAHNVAADVYDAMQLTEREAYDAIALTGALPLYDPRF
                     ERALKVGGRLFATVGTGPLLEARRITRVGHDEWVRESLFETSLTPLVHAPEPPRFVF"
BASE COUNT          855 a         2067 c         2147 g          962 t
ORIGIN      
        1 caaggagcgc gccggcgagt tcgactcgcg cttcatcgag ctcgcgcgcg cggtctacgt
       61 caccaacgac gagcgcgccg ccgccaagcg gcgcatcaac accaggctcg gctcggcgct
      121 ggtggaggag aagagctaca aggaatacaa gagaagtggc tagtggctag tggctagtgg
      181 ctagttaaga aaagcaaggc cggcgcgatc gccggccttc tcactgacca ctaaccactg
      241 accactagcc actaaccact ggctactaac ctccgaccac tttcctcagc gtgtattccg
      301 cgctgcagta cgggcacttc gcccagccgg ttttctcgac ggctaagaac acgcgcgggt
      361 gagaattcca caggtgcatg ccgggcatgg ggcagctgag cggcaggtcc gccgcggtca
      421 cgtcgtactg atgctgggcg ttcggctgga tgagcttttc gctgcgatcg gctgccgtca
      481 cggtaggggg cctcgataaa gccacgggtc gatggttcga acggggccgg cgggccgccg
      541 ccgccgcatt atcgcaaaat cggctcggcc gggcgggtgg gaccggttct cggggcgccg
      601 gccgccatca gctcgtcgag cttctccacc gcgtcgtcga cttcgatcag gtccatcacg
      661 ccgggccgct cgatcttctc cgtccagggc acgcggctgg cggatcggtt caggtaccgg
      721 gtcgcggccc gctcgtagcg gtcgacgcac cactgtcgac tgtaataggg gccgctgcgc
      781 tgcggattcg tggccgcgta cagcccgacg accggcgtgc ccaccgtcgt cgccatgtgg
      841 gccggaccgg agtcgggcga cacgagcgcg gtcgcgcgtt gcagcgtagc caggaactcg
      901 agcagcgtgt cctggccgat caggttgcgc gacggctggc gcatgcagcg ctcgatgtca
      961 cgtccgtagc ggagctcgag gtcgctgcgg cccccgcaca gcagcacctc catgccgtgg
     1021 cgcacgacgg cgtgatcggc gagcgccgca taacgttccg cactccagtt gcgcgatgcg
     1081 tggctcgagc acgggctcag gaccagcgtc ggccgcgacg tgtcggggat ggcgcgcccc
     1141 gcgtactcga gcgccgtgtc cggcagcggg atgtcccagc gcatcgagcg cgtccccagc
     1201 cccaggcgtt ctgcaaaccc gaacaaggag tcgagcacgt gctcgcgagt gcgcggctcg
     1261 atctgcacgt tggtgaacag ccattggccc tcacgtgcgc gcccgcggtc gaagccgacc
     1321 ttccagcgcg ccggcacgag cgccgcggcg aggctcgcgc gcagtgcgag ctgcatgtgc
     1381 agcagcgcgt cgaagcgacg gcttgccagg cgctcgcgca aggcccggta gccggcggca
     1441 ccggccttct tgtcgaacac gacgaactcg acgccgggaa cgtggccgag cagcttgtgc
     1501 tcgaccttgc cgaggatcca ggtgaggcgc gtctgtggcc aggcgtcctg cagcgtgcgc
     1561 accaccggca gcacgtggca cacgtcgccc agggcggaca gccgcaggat gcagacggac
     1621 tcaggcgggc tcgtgaacag gggctgcacg gaggtctcga cggtaggcag cggcgccggg
     1681 atcgggaaca atcggcgacg atgaacccag ccgccgtcgc cggtgacgaa gcgctcgcgc
     1741 gaattgcggg cggcgcgatg ctatacgatg ccgcgcgcgt cggccaaccg gaggccgcga
     1801 tcttcgagcc ggcgtactgg caggcgcgcg gcgcggcggt gggcactcgc ggtggacgtg
     1861 gcacggtgac gttcgtgcgt ggcgcggacg gcgagccgtg ggtcctgcga cactaccggc
     1921 gtggcggtgc cgtcgcgcga ctgagcagcg acgcgtatct ctggaccggt gcacctcgca
     1981 cgcgctcgtt cgccgagtgg cggctgctgc gccagctgcg cgactggaac ctgcccgtgc
     2041 cgcgaccgat cgcggcgcgc tacctgcggc gcgggctcac gtatcgtgcg gacctgatca
     2101 ccgaggagct gccgacgcgt cgcacgctcg cggcgtcgct cgccgacgcg ccgttgccga
     2161 cggccgcctg gcacgcaatc ggtcgctgca tcgcgcggct gcacgcgcgc ggcgtgcacc
     2221 acgcggacct gaacgcgcac aacctgctgc tcggcgacga cggtgcggtc tacgtgctcg
     2281 acttcgaccg cggacgcatc cgtcgtcgcg gctcctggga acgcgtcgtg ctcgcgcgtt
     2341 tgcggcgttc actcgacaag gtgacggcga agtacccgcc cggtcgcttc ggtcacgagc
     2401 aatggcgcgc cttgctcacc ggagtcgagg aatgacccgc cgatgatgcg cctgctctac
     2461 gtcgcgctca cgtacctgct cgcgccggtc ttcatcgtcc gcgaggcctg gcaggcgtgg
     2521 cgcgatcgct cacagcgtgg ccgtgtcgcg caacgcctcg gctacgtggc gcgcgcgccg
     2581 cgcgacgccg acgtgtgggt gcacgccgta tcggtcggcg aagtgcaggc agcggcggca
     2641 ctcgtgcggg cgctcaggcg ccggcgaccg gacctggcga ttgcgctcac gacgacgacg
     2701 gccacggggg cccagcgtgc gcacgcgttg ttcggagata ccgtgcacca cggttatctg
     2761 ccgtacgacc tgcccgggcc cgtgcgccgc ttcctcgatc gggtctcgcc tcgggtggcc
     2821 gtgatcctcg agaccgaaat ctggccgacg ctgtacgcga cactcgcgca ccggcgcatc
     2881 ccgctggtgc tcgcgagcgc gcggctctcc gcgcgctcgg tcgaccgcta tcggcgcttc
     2941 gccgcgctgt tcggcgaatc gctcgcgcag cacgtcgcga tcggtgcgca gaccgccgag
     3001 gatgccgagc gcttccgacg gatcggcgcg ccggcagagc gcgtgcaggt cactggcaac
     3061 gtcaagtacg acctcgaaat accggccggg caggtcgagg cgggtcgcgc gttgcgcgag
     3121 cgttggggac gtgaacggcc ggtgtggatc gccggcagca cgcacgaagg cgaggaggcc
     3181 gcggcgctcg cagcgcacga ggccgtgctc gaacgctttc cggatgcgct gttgctgctc
     3241 gtgccgcggc acccgccgcg cttcgaggcc gtgcgggcgt tgctgcagcg gcgcggtgtc
     3301 cctttcgcgc gacgcggtgc aggcgcggtg ccggcaccga cgcagaccgt gtacctcgtg
     3361 gacacgctcg gtgaactgca ggcgttctac gccgcgagcg acgtcgcgtt cgtcgggggc
     3421 agcctcgtgc cgatcggcgg gcacagcctg ctggagcctg ccgtgctcgg cgtgccgata
     3481 ctgtccgggc cgtacacgca gaacggccgg gagattgccg aactgctcga gcgagccggc
     3541 gcgctcgagc tggtcgccga cgcgccggcc ctcggccgcg cgctgctcgc gtggttcgag
     3601 gcgccgtccg tggcgcgtga cgcgggcgag tcaggccggc gtgcggtcgc ggccaatcgt
     3661 ggcgcggtcg accggctggt cgggatgatc ttgccgcggc tggtcaggtc ttcggacgca
     3721 ccaccggcag cgtcgtcggt gccgtgagga agctgttgat cctcgcgagg tcgtcctgca
     3781 cgaggccacc cgcggccgat tccagccgga cgatgttgat caggtagtcg tagcggctgc
     3841 gggcatagtc gcgctgggcc tcgaacagcg tgcggcgcgc cgcgagcacg tcgaccgtgg
     3901 tgcgcgtgcc cacctcgaaa ccggcctcgg tggcctcgag cgccgtctgg ctcgacttca
     3961 cggcctgggc gagcgcctgc acgcgggcct tctcggcgat gacgccgagg taggcgtcgc
     4021 gggtctcgcg ctcggccgag cgcatcgagc cttcgaggcg ttcgcgcgcc gcgcgctgca
     4081 ggtacaccga ctcgcgcacg cgtgactgcg tcacgccgcc gctgaagatc gggatcgtga
     4141 cctgcacgcc gatcgtatcg tcggtctgcg tcgagtcggc cgggccggtc aggccattgt
     4201 tcgtggcggt ggtgtcgccg tcgaactcgc tgcgccgcgc gaacaggtcg atcgccgggt
     4261 aatgtccggc acgcgccacc ttgacgtcgc gcgcggcgac ttccgtctcg agtcgcgccg
     4321 cgatgacgga gatgttgttc tccagcgcgc ggtcgaccca cgcctgctcg tcggccgggt
     4381 tcggcgcgtc gagcggcatc tgttccgcag gcttggccaa ggtggtgtac gcctcgttgg
     4441 tgagttcgcg cagcgcctcc tgggtcgtcg cgagggtgcg cttggccgcg atgacgcccg
     4501 ccgtcgcgct gtcgtgcgcc gcacgcgctt cctgcacgtc ggtgatcgcg atcagcccga
     4561 cctcgaagcg cttgtccgcc tgctcgagct gccgcgtcag ggcctcgagc gtcgcctcgg
     4621 ccgccgcgag cgtgtcctcc gcggcgagca cgtcgaaata cgccgtgctc acgcgcacca
     4681 gcaggttctg ctgggcggca cggaaattgg cttcggccac ggccacctgc gcgtcggcgc
     4741 gcttcagcgc ctgccactgg tcccagcgga aaagcgactg cgtgatctgc gcgtcgtacg
     4801 accagaagtc ggagtcgacg gtctgattgt tgggcacctg cacgatgatg ttcggcgtcg
     4861 cctcgttgtc gtcgtggtcg acgctttgcg tgaaggtgga ttcgctttcc gagtcgcgag
     4921 tcgcgtactg cccgctcacg tcgacctgcg gcagcagcag tccgcgggcc tgcggtttcg
     4981 cctcgagcgc cgccatccgg cgcgcctcgg cttcgcgaag cacgggatcg ctgctcagcg
     5041 cgtcctggta cacgtcgatc aggttctccg cgcgcacggg catgcccgcc acgagcacag
     5101 cggcgacgct gcaggcaaga agcagcgatt ttcttgtcat ggactcactc actctgcggc
     5161 gtcgccgcgc gtggtcaact agtgttatac tagactatat caccggccga tcggtaggct
     5221 agcccctgtg ccgggtggat gcccgccgcc gatcgacgaa ttcgcccggc tggtcgcgtc
     5281 agaacacgaa cctgggcggt tcgggcgcgt ggaccagcgg cgtcagcgag gtttcgaaca
     5341 ggctttcacg cacccactcg tcgtgtccca cgcgcgtgat ccggcgggct tcaagcagcg
     5401 ggcccgtgcc gacggtcgcg aacagccgtc cacccacctt cagcgcccgc tcgaagcgcg
     5461 ggtcgtacag gggcagcgcc ccggtgagcg cgatcgcgtc gtaggcctcg cgctcggtga
     5521 gctgcatcgc gtcgtacacg tcggccgcga cgttgtgcgc cccgaccctg cgccaggtcg
     5581 cctcggcttt cggcacgaac tcggcgtgga tgtcgatcgt acgcacactg cgggcgagct
     5641 ggccgatgca ggcggcgaag aagcccgtcc cggtgccgac ctcgagcacg tggtcgcccg
     5701 gttgcaggtc gagcgcctgc aggatgcgac cctccacctt gggaggcagc atcgcctgtc
     5761 cgtgtcctat cggcacggag gtgtcggcga acgcaagctc gcggtaggcc gcaggcacga
     5821 attcctcgcg cggcacgcgt tcgagcgcct cgagcaccct gaggtcgagc acctcccacg
     5881 cgcgcacctg ctgctcgatc atctggcggc gtgcggactg gacgtcgaac attcgagagg
     5941 ttccggatga gaaaacgcga atcggcgctt ccgaccggcg cgaaggttaa ggttggacat
     6001 cgcgcggaac aagccgaatc aatgcgcatt a
//
LOCUS       DASPFR010000203         3300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1523700, whole genome shotgun sequence.
ACCESSION   DASPFR010000203 DASPFR010000000
VERSION     DASPFR010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523700"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2034
                     /locus_tag="VD737_05205"
     CDS             <1..2034
                     /locus_tag="VD737_05205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmcA/MtrC family decaheme c-type cytochrome"
                     /protein_id="HXW09991.1"
                     /translation="IADIGTLVATIDSATITDNKPVLEITVQTAHGGDVLGLAATTLR
                     LGIAKLVPAGAGLPSRWQSYVNRAGTPSIQNPTLASAIQANVESGVAAGWQELGEGRY
                     RYTSAVNLASVTTPIAVPYEPTLLHRLSLALDLSGNARALAPDNPHVDFVPAGGTVIG
                     KDIAATEKCTGCHVRFAEHGGPRRSVEFCVVCHNPGSVDPDRGESVDMAYMAHSIHRG
                     EARAQPYVVYGFGGTEYDFSHVTYPQPLAFCETCHEQSDAAPQGDDWSTTVTASACGG
                     CHVAGMNKTGPDPATGRYAYTYTHSNTLVPPQAFQDGDCLGCHGAGKVAGTTLALHQR
                     DEDRKAIEQGDLFTYRILNIENAAVGQQPKVTFQILDANGAPVDVKAITTGRLRLDFS
                     WSAPDVHNVAEVAGNAYAANRGEAIVIDLIAGMASVVANGDGTYSYTLSTPLPTGFAD
                     AALGSGLLVVLEGRRQMPDGSEAYPESAFRFSGGTARENLVAQEKCETCHKRVAAHGG
                     SRAGDPLVCTVCHNSSVGGTFGTDVVGPLALGAFIHNVHADKVAGVGEVTYPQSLARC
                     EGCHLAGKFNVARTSALPITVDAGTTATSGAETLAWQDDLADSATAGTCRTCHDAPEA
                     IAHMQQQGGSFAQPKTLSPSSAQEGCSTCHAAGRTFDTAAAHCGALPFGQCTQ"
     gene            2129..>3300
                     /locus_tag="VD737_05210"
     CDS             2129..>3300
                     /locus_tag="VD737_05210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmcA/MtrC family decaheme c-type cytochrome"
                     /protein_id="HXW09992.1"
                     /translation="MNEKYTSLGWLARGLLVAGLGLATVGLGGCSGDNSSVEPPSPPA
                     GPFPPQPGTGAPVPVTTATQIVATVQRVTVPEDGKPVVEVYLKDEEGFSLTGLPAANI
                     RFVLARLEPAQNGASSTWHAITRRTEAFPGTPTPVPAEAVTGTGPASQGYTETATAGV
                     FTEVGNGVYTYQFAQGLKDNAAIPFDGALPHRVGLEIRIGSPTIPANNAVMTFNPVTG
                     TPIAASGREIVDNDTCNACHDNLVFHGGARFDLQYCVMCHDSYSSDAQTGNTIDLKVM
                     IHKIHAGETLPSVEAGGLYGIFGNNNSWNDFSHVVYPQDKRNCTTCHEEGDADTPQAS
                     NWRLTVNRTTCSSCHDDENFTTGANHGGIAATDETCSACHGPGSGVLSVQTAHQIP"
BASE COUNT          575 a         1196 c         1097 g          432 t
ORIGIN      
        1 atcgccgaca tcggcacgct ggttgccacg atcgattccg ccaccatcac ggacaacaag
       61 ccggtgctcg agatcacggt ccagacggcg cacggcggcg acgtgctcgg cctggccgcg
      121 accacgttgc gtctcggcat cgccaagctg gtgccggccg gcgccggcct gccgtcgcgc
      181 tggcaaagct acgtgaaccg ggcgggcacg cccagcatcc agaacccgac gctcgcgagc
      241 gcgatccagg ccaacgtcga gagcggtgtc gctgccggtt ggcaggaact cggcgaagga
      301 cgttaccggt acacctcggc ggtcaatctc gccagcgtga cgacgccgat cgccgtgccc
      361 tacgagccca cgctcctgca tcgactgagc ctcgcgctcg acctgtctgg caacgcgcgc
      421 gccctcgcgc ccgacaatcc gcacgtggat ttcgtgccgg cgggcggcac ggtcatcggc
      481 aaggacatcg ccgccacgga gaagtgcacc ggctgccacg tgcgattcgc ggagcatggc
      541 ggcccgcgtc gcagcgtcga gttctgcgtc gtctgccaca accccggttc ggtcgacccc
      601 gatcgcggcg agtccgtgga catggcgtac atggcccact cgatccaccg tggtgaggcc
      661 cgcgcgcagc cgtacgtcgt ctatgggttc ggcggcacgg agtacgattt ctcgcacgtg
      721 acctacccgc agccgctcgc cttctgcgag acctgccacg agcagtccga cgccgcgccg
      781 cagggcgacg actggtcgac cacggtgacc gcgtccgcct gcggtggctg ccacgttgcg
      841 ggcatgaaca agaccgggcc tgatcctgcg accggccgct acgcatacac ctatacgcac
      901 tccaacacgc tcgtgccgcc gcaggctttc caggacggcg actgcctggg ctgccacggc
      961 gccggcaagg tcgcgggcac aacgctcgcg ctgcaccagc gcgacgagga ccgcaaggcg
     1021 atcgagcaag gcgatctctt cacgtaccgg atcctcaaca tcgagaacgc ggccgtgggc
     1081 cagcagccca aggtgacgtt ccagatcctc gacgcgaacg gcgcgccggt cgacgtgaag
     1141 gccatcacga cgggccgcct gcggctcgac ttctcgtggt ccgcgcccga cgtccacaac
     1201 gtggcggagg tcgcgggcaa cgcgtacgcg gccaatcgtg gcgaagcgat cgtgatcgac
     1261 ctgatcgccg gcatggcgag cgtcgtcgcg aacggcgatg gcacgtacag ctacacgctg
     1321 tcgacaccgc tgccgaccgg gttcgccgac gccgcgctcg gatcgggcct gctggtggtc
     1381 ctcgaagggc ggcgccagat gccggacggc tcggaggcgt acccggagag cgcgttcagg
     1441 ttctcgggcg gcacggcccg tgagaacctc gtcgcgcagg agaagtgcga gacctgccac
     1501 aagcgcgtgg ccgcgcacgg cggcagccgc gcgggcgatc cactggtctg caccgtctgc
     1561 cacaactcga gcgtcggtgg cacgttcggc accgacgtcg tcgggccgct cgcgctcggc
     1621 gcgttcatcc acaacgtgca cgcggacaag gtcgcgggcg tcggtgaggt cacctatccg
     1681 cagagcctcg cgcgctgcga gggctgccac ctcgcgggca agttcaacgt cgcccgcacg
     1741 agcgcgctgc cgatcacggt cgacgcggga acgacggcga cgtccggtgc ggagacgctg
     1801 gcctggcagg acgacctcgc agactcggcc acggccggca cctgcaggac ctgccacgac
     1861 gcgccggagg cgatcgcgca catgcagcag caaggcggct cgttcgcgca accgaagacg
     1921 ctgtcgccct cgtcggcgca ggaaggctgc agcacctgcc acgccgcggg gcgcacgttc
     1981 gacaccgcgg cggcgcactg cggtgcattg ccgttcgggc agtgcacgca gtgagcagca
     2041 gcaagagttc gaacaacgac ggatctgcgc gccggacaag ccccggcgca cggccgcaca
     2101 cggccgaagg acaggctgga gtcgcacgat gaacgagaag tacacgtcac tcggatggct
     2161 ggcgcgcgga ctgctggtcg cgggcctcgg gctcgccacc gtcggcctcg gcggctgcag
     2221 cggcgacaat tcgagcgtcg agccaccgtc gccaccggca ggcccgttcc cgccgcagcc
     2281 cggcaccggg gcaccggtgc cagtcaccac ggccacgcag atcgtggcga ccgtgcagcg
     2341 cgtgaccgtg cccgaggacg gcaagccggt cgtcgaggtc tacctcaagg acgaagaagg
     2401 tttttcgctc accggcctgc cggcggcgaa catccgcttc gtgctcgcgc ggctcgagcc
     2461 ggcgcagaac ggcgcctcga gcacctggca cgcgatcacg cgtcgcacgg aagcgttccc
     2521 gggcacgccc acccccgtac cggccgaagc cgtgaccgga accggtccgg ccagccaggg
     2581 ctacacggag acggccaccg cgggcgtctt caccgaggtc ggcaacggcg tctacacgta
     2641 ccagttcgca cagggcctga aggacaacgc cgcgattccg ttcgacggcg cgctgccgca
     2701 tcgcgtcggt ctcgagatcc ggatcggcag cccgacgatc ccggccaaca acgccgtcat
     2761 gacgttcaac cccgtcaccg gcacgccgat cgccgcgtcg ggccgcgaga tcgtcgacaa
     2821 cgacacctgc aacgcctgcc acgacaacct cgtgttccac ggcggcgcgc gcttcgacct
     2881 gcagtactgc gtgatgtgcc acgactcgta ttcgagcgac gcacagaccg gcaacacgat
     2941 cgacctcaag gtgatgatcc acaagatcca cgcgggcgag acgctgccga gcgtcgaagc
     3001 gggcggcctc tacggcattt tcggcaacaa caactcgtgg aacgacttca gccacgtcgt
     3061 ctacccgcag gacaagcgca actgcaccac ctgccacgag gaaggcgacg ccgacacgcc
     3121 gcaggcgagc aactggcgcc tgacggtcaa tcgcaccacc tgctcgtcgt gccacgacga
     3181 cgagaatttc acgaccggcg cgaaccacgg cggcatcgcg gcgaccgacg agacctgcag
     3241 cgcctgtcac ggtccgggtt ccggcgtgct cagcgtgcag accgcgcacc agattccgga
//
LOCUS       DASPFR010000204         7124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_546395, whole genome shotgun sequence.
ACCESSION   DASPFR010000204 DASPFR010000000
VERSION     DASPFR010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546395"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1375)
                     /locus_tag="VD737_05215"
     CDS             complement(<1..1375)
                     /locus_tag="VD737_05215"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HXW09993.1"
                     /translation="MPSIVRPRTWAGLLCGVLLMAAQGAQDAQAAPSYDDLVELWQQW
                     RTFERPPLRDGAPDYTAAAVAQRHAELRRYQERLEAFDTAGWTREQRIDLELVRAQLN
                     GMDFDLRVLRPWARDPAYYQSIWTEQSDTPEHEGPAHHGLVELWQYTYPLSAADAGKL
                     AQQLRIVRPLLEQARGNLTGNARDLWVTGVGTMQAQVKNLDDLAQKTQGAGDELARAI
                     AEAREATVAFVAWLEQQAPQKNGPSGIGKEAYEWNLKHVHLVPMTWDEEVALLKRELA
                     RAHAALRFEEHRNRGLPALTTPADAEAYAKKANDAVTKYMAWLARKQVFTVEPYLEPA
                     LRRHIDPFVPPESRNFFSIASHYEPITLYAHFQHWFDHAWLVHAPNASPIRRTAWYYN
                     AWDSRAEGMATAMEEIVLHAGYYDDHPRAREIVWIMLAQRAARGLASLYAQANDFDIA
                     AAKAFQVE"
     gene            complement(1567..2187)
                     /locus_tag="VD737_05220"
     CDS             complement(1567..2187)
                     /locus_tag="VD737_05220"
                     /inference="COORDINATES: protein motif:HMM:NF022426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HXW09994.1"
                     /translation="MKTPLSVVVLTVALAGCASMSKEECRYVDWRTVGYEDGASGRPA
                     SRLGDHRRACADHGVTPDLTAYEAGRAAGMREFCQAHNGYRVGASGQVYYGTCPADLA
                     PAFERGYDQGRELYVREQRVRDAENGIAYREAEIRRLEDGLARGAFVLVGNTSTAEER
                     TQAVIDAKQAAERIGRLRKEIEGLQADRLRYERELEVYRSTVASTY"
     gene            complement(2335..3069)
                     /locus_tag="VD737_05225"
     CDS             complement(2335..3069)
                     /locus_tag="VD737_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXW09995.1"
                     /translation="MSAGVLARVLALGALVAFLVVPQWFAPALAPLNQYGAPPIYDQG
                     DLLPLALWHLGTVVVAAVASTLVAVSLGICVTRPAGARFLPLSRALVDFGQTFPPVAV
                     LAIAVPLVGFGQVPTLIALFAYGLLPVFENTIAGLEGCPRAALDAADGMGMSPRQRLL
                     DVELPLALPTILEGIRLSLVINVGTAAIGSTVAARGLGEVIIAGLLSNNTAFVLQGGL
                     VTALLAILLYDALRLLERRLVRGRPG"
     gene            complement(3066..4004)
                     /locus_tag="VD737_05230"
     CDS             complement(3066..4004)
                     /locus_tag="VD737_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXW09996.1"
                     /translation="MITIEKLSKRYGEVTAVDEVSMTVEPRTITTIVGTSGSGKSTLL
                     RMINRLVEPSSGRVLIDGRDTALERPHLLRRRIGYAIQGFGLFPHRTVAENVATVPRL
                     LGWDTVRIDARVTELLAACDLEPDLYAGKYPHELSGGQQQRVGVARALAAAPDLLLMD
                     EPFGALDAILRAKAQDDLLAIQRRFGTTIILVTHDIDEAFRLGDRVAVMRDGRLLQHD
                     RPARLVTQPADPFVAQLAGTGERSLRLLALLGTSGVARPGSVEGPAVKATASLREVLA
                     ELVWRGATSANVVDEAGVSVGHVALADVLARGRQAS"
     gene            complement(4001..5173)
                     /locus_tag="VD737_05235"
     CDS             complement(4001..5173)
                     /locus_tag="VD737_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXW09997.1"
                     /translation="MIARGVPRGPGLDPLGVVACAIGAVALATLPFVLFKANRILPGE
                     PRAIADVLPATLAWGFHALLALVAVVALRARNAHLRLVVMLAGLAATAMVVAVAASAL
                     TPDGNRVVRAAPGGGAWVLVILLGLLATDALTRLQLGPLARVACMVAFLVAVVAALSS
                     GYFDDLSIMREYANNAARFADEARRHAALAIGSLLAALIVGVPTGVACHRAPRLRAAV
                     LGALQVVQTVPSIALFGILMAPLGALVAAWPALASAGVSGIGAAPAAVALFLYALLPV
                     VANTVVGLARVPESAVDAARGLGFTPLQVLREVELPLALPTMLAGVRIALVQNVGLVT
                     IAALVGGGGFGTFVFQGIGQTAMDLVLLGALPTVAFAYSAAVVLEALVERSQRVPR"
     gene            complement(5170..6108)
                     /locus_tag="VD737_05240"
     CDS             complement(5170..6108)
                     /locus_tag="VD737_05240"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HXW09998.1"
                     /translation="MAQWLRALTLAMGLCAGTHVAATTAASRPIVVSSKLSSESVMIG
                     QMIRRLLEAHGIATLDRTALGGTPLVRRALLAGEIDLYVEYTGNAGFFFNRPGDAAWK
                     DWRRGYELGARLDREQHGIVWLTPAPASNAWALAVRRDVARRHRLATLSDFARWVREG
                     GRAVLACSAEFANAGTLRSLERTYDFHLEPRQKIVLAGGETAATIAAAAARTNDVNTA
                     MVYGTDGGIAAADLVLLEDDRHDQPVYAPVPTARQAVVSAYPQLPAIVRPLMQRLDAA
                     TLQALNARVQIDGESPEAVAVDFLQGAGLLAAGSGR"
     gene            complement(6223..6675)
                     /locus_tag="VD737_05245"
     CDS             complement(6223..6675)
                     /locus_tag="VD737_05245"
                     /inference="COORDINATES: protein motif:HMM:NF021082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2383 domain-containing protein"
                     /protein_id="HXW09999.1"
                     /translation="MSSTDILGDLNELLRDELAAIETYRHAIERVRKTHGEDPRFGKL
                     TDMLRDHELAASKLRELVHRLGGTAANDPGPWGTWANTVLNAASLLGDKAALRALREG
                     EESGVKDYRAALTEVTAVTTPEVLDVYAWNLAREEEHVRQLDRLIAFG"
     gene            6896..>7124
                     /locus_tag="VD737_05250"
     CDS             6896..>7124
                     /locus_tag="VD737_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10000.1"
                     /translation="MESADEHHVSPDVASVGSLHWRYLDQYGGEVLCAAADRRPVVFR
                     TVYEAVETSRRLGGCSLGRCLHAPMRWVLLRP"
BASE COUNT         1031 a         2546 c         2542 g         1005 t
ORIGIN      
        1 actccacctg gaaggccttg gcggcggcga tgtcgaagtc gttggcctgg gcgtagagcg
       61 aggccaggcc gcgcgcggcg cgctgcgcca gcatgatcca cacgatctcc cgggcgcgcg
      121 ggtggtcgtc gtagtagccc gcgtgcagca cgatctcctc catcgcggtg gccatgccct
      181 cggcgcggct gtcccaggcg ttgtagtacc aggcggtgcg acggatcggg ctcgcgttcg
      241 gcgcgtgcac gagccaggcg tggtcgaacc agtgctggaa gtgcgcgtac agcgtgatcg
      301 gctcgtagtg gctcgcgatc gagaagaagt tgcggctttc gggcggcacg aacgggtcga
      361 tgtgcctgcg cagcgccggc tcgaggtagg gctcgacggt gaagacctgc ttgcgggcga
      421 gccaggccat gtacttggtg accgcgtcgt tggctttctt cgcgtaggct tccgcgtcgg
      481 ccggcgtcgt gagggcaggc aggccgcggt tgcgatgctc ctcgaacctc agcgccgcgt
      541 gcgcgcgcgc cagttcgcgc ttcaacagcg cgacttcctc gtcccaggtc atcgggacga
      601 ggtgcacgtg cttgaggttc cactcgtagg cttccttgcc gatgcccgac gggccgttct
      661 tctgcggggc ctgctgctcg agccaggcga cgaacgcgac cgtcgcctca cgcgcctcgg
      721 cgatggctcg tgccagctcg tcgccggcgc cctgcgtctt ctgcgcgagg tcgtcgaggt
      781 tcttgacctg cgcctgcatc gtgccgacgc ccgtgaccca gaggtcgcgc gcgttgcccg
      841 tcaggttgcc ccgcgcctgc tcgaggagcg gacgaacgat gcgcagctgc tgcgcgagct
      901 tgcccgcgtc ggcggcgctc agcggatacg tgtactgcca gagttcgacc aggccgtggt
      961 gggccgggcc ttcgtgctcc ggcgtgtcgc tctgctcggt ccagatcgac tggtagtacg
     1021 ccgggtcgcg ggcccagggg cgcagcacgc gcaggtcgaa gtccatgccg ttcagttgcg
     1081 cgcgcaccag ttcgaggtcg atgcgctgtt cgcgtgtcca gcccgcggtg tcgaaggctt
     1141 cgaggcgttc ctggtaccgc ctgagctcgg cgtgccgctg cgcgaccgcg gcggcggtgt
     1201 agtccggcgc gccgtcccgg agcggagggc gctcgaacgt gcgccactgc tgccacagct
     1261 cgacgaggtc gtcgtacgag ggggcggcct gcgcgtcctg cgcaccctgt gcagccatca
     1321 ggaggacacc gcacagcagg ccggcccacg tgcgcgggcg gacgatcgac ggcatcggag
     1381 agttcctttc cgccggcacg ggcggtcgat ggacggcatg ccggaaaaag taccagaaat
     1441 ggggacactc cgaattaacg gctaaatggg gacactcctg atttcgtgcg ggggcggaaa
     1501 aacgtggcct ggcgaacggc cctggaaatc aggaatgtcc ccaatttccg gggtgtgcct
     1561 gggcggtcag taggtcgagg cgacggtgct ccggtagacc tcgagctcgc gctcgtagcg
     1621 gaggcggtcc gcctgcagcc cctcgatctc cttgcgtagc cggccgatgc gctcggcggc
     1681 ctgcttggcg tcgatgacgg cctgcgtgcg ctcttccgcc gtcgaggtgt tgccgaccag
     1741 cacgaacgcg ccgcgcgcga gcccgtcctc gagccggcgg atctcggcct cgcgataggc
     1801 aatgccgttt tcggcgtcgc gcacgcgctg ttcgcgcacg tagagctcgc ggccctggtc
     1861 gtagccacgt tcgaacgccg gtgcgaggtc cgcggggcac gtgccgtagt acacctgccc
     1921 gctggcaccc acgcgataac cgttgtgcgc ctggcagaac tcacgcatgc ccgccgcgcg
     1981 acccgcctcg taggcggtga ggtccggcgt gacgccgtgg tccgcgcagg cccgccggtg
     2041 atcgccgagc cggctggccg ggcggccgga cgcgccgtct tcgtagccga ccgtgcgcca
     2101 gtcgacgtag cggcactctt ccttgctcat cgacgcgcac ccggccagcg cgacggtgag
     2161 cacgacgacg gacaacgggg tcttcatcac gacgcggtca ctcctgatca cccgcatcgc
     2221 agcataagga tcgcgccgcg actggcgcgt gcggcggtca cggaatcgat cgacgaccgg
     2281 ccccgatttc acttaacgtc gcgccggcca cgccgtgatc gggcgggcgc gcggtcaacc
     2341 cggacggccc cggacgagcc tgcgctcgag cagccgcaac gcgtcgtaca gcaggatcgc
     2401 gagcagcgcc gtcacgagcc caccctgcag cacgaaggcg gtgttgttgg acagcaggcc
     2461 cgcgatgatc acctcgccga ggcccctggc cgccaccgtc gagccgatcg ccgccgtgcc
     2521 gacgttgatc accagcgaca gccggatgcc ctccaggatc gtcggcagcg cgagcggcag
     2581 ttcgacgtcg agcagccgtt gccgtgggct catgcccatg ccgtcggccg cgtcgagcgc
     2641 ggcccgcggg cagccctcga gcccggcgat ggtgttctcg aacacgggca gcagcccgta
     2701 cgcgaacagc gcgatcagcg tcggcacctg cccgaacccg accagcggaa ccgcgatcgc
     2761 aagcaccgcg accgggggaa acgtctgccc gaagtcgacc agcgcgcgcg agagcggcag
     2821 gaaccgggcg ccggccgggc gcgtcacgca gattccgagc gagacggcga cgagcgtgct
     2881 ggcgaccgcc gcgacgacga cggtcccgag gtgccacagt gcgagcggca gcaggtcgcc
     2941 ctggtcgtag atcggcggcg cgccgtactg gttgagcggc gccagcgcgg gggcgaacca
     3001 ctgcggcacc accaggaacg cgaccagcgc gccgagcgcc agcacgcgcg cgaggacgcc
     3061 tgcgctcacg acgcctggcg tccgcgtgca aggacgtcgg ccagcgcgac gtgcccgacg
     3121 gacacgcccg cctcgtcgac gacgttcgca ctcgtggcgc cccgccagac cagctcggcc
     3181 agcacctcgc gcagcgacgc ggtcgccttc acggccggcc cttcgacgct gccgggccgc
     3241 gccacccctg acgtcccgag caatgcgagc agtcgcagcg agcgctcgcc cgtgccggcg
     3301 agctgcgcga cgaacgggtc ggccggctgc gtgaccaggc gggccggccg gtcgtgctgc
     3361 agcagccgtc cgtcgcgcat gaccgccacg cggtcgccga gcctgaacgc ctcgtcgatg
     3421 tcgtgcgtca ccaggatgat ggtcgtgccg aaccgacgct ggatcgccag cagatcgtcc
     3481 tgcgccttcg cccgcaggat cgcgtcgagc gcgccgaacg gctcgtccat caacagcagg
     3541 tccggcgcgg cggcgagcgc gcgcgccacg ccgacccgct gctgctgtcc gccggacagc
     3601 tcgtgcgggt acttgccggc gtagagatcg ggctccaggt cgcacgcggc gagaagctcc
     3661 gtcacgcgcg cgtcgatgcg caccgtgtcc cagccgagca gccgcggcac cgtggcgacg
     3721 ttctcggcga ccgtgcgatg ggggaaaagc ccgaaaccct ggatcgcgta gccgatccgt
     3781 cgtcgcagca ggtgcggccg ctcgagtgcg gtgtcgcggc cgtcgatcag cacgcggccg
     3841 gaggagggct cgaccagccg gttgatcatg cgcagcagcg tcgacttgcc ggaacccgac
     3901 gtgccgacga tcgtggtgat cgtgcgcggc tcgacggtca tcgacacctc gtcgaccgcg
     3961 gtcacttcgc cataccgctt gctgagcttc tcgatcgtga tcatcgcggc acccgctgcg
     4021 accgttcgac gagcgcttcg agcacgacgg cggccgagta cgcgaacgcg accgtcggca
     4081 gtgcgccgag cagcacgagg tccatcgccg tctggccgat cccctggaac acgaacgtgc
     4141 cgaatccgcc gccgccgacc agtgctgcga tcgtcacgag gccgacgttc tgcaccagcg
     4201 cgatccgcac gcccgcgagc atcgtcggca gggcgagcgg cagctcgacc tcgcgcagca
     4261 cctgcaacgg cgtgaacccg aggccccgcg cggcatcgac cgccgactcc ggcacgcgcg
     4321 cgagcccgac caccgtgttg gccacgaccg gcagcagtgc gtacaggaac aacgcgacgg
     4381 cggccggcgc ggcgccgatg ccgctgacgc cggcgctcgc gagcgcgggc cacgccgcga
     4441 ccagcgcgcc cagcggtgcc atcaggatcc cgaacagcgc aatggacggc acggtctgca
     4501 cgacctgcag ggcgccgagc acggcggcgc gcaaccgtgg cgcccggtga cacgcgacgc
     4561 cggtcggcac gcccacgatc agtgccgcga gcagcgagcc gatcgcgagt gccgcgtgcc
     4621 gtcgtgcctc gtccgcgaac cgtgcggcat tgttcgcgta ctcgcgcatg atcgagaggt
     4681 cgtcgaaata cccggacgag agcgccgcca cgaccgccac gaggaaggcg accatacacg
     4741 cgacgcgcgc cagcggcccg agctgcaacc gcgtcagcgc gtccgtggcg agcaggccga
     4801 gcaggatcac gagcacccag gcgccacccc cgggggccgc gcgcaccacg cggttgccgt
     4861 cgggcgtcag cgcgctggcc gcgaccgcga cgaccatggc cgtcgccgcg agaccggcga
     4921 gcattacgac gagccgcaga tgggcatttc gcgcccgcag ggcgacgacc gcgacgagcg
     4981 cgagcagcgc gtggaacccc cacgcgagcg tcgccggcag cacgtccgcg atcgcgcgcg
     5041 gttcgccggg caggatgcgg ttcgccttga acagcacgaa cggcagcgtg gccagcgcga
     5101 ccgcgccgat cgcgcacgcg acgacgccga gtgggtcgag cccgggccca cgcgggacgc
     5161 cgcgcgcgat caacggcccg acccggcggc cagcaggccc gcgccctgca ggaagtccac
     5221 cgcgacggcc tcgggtgact ctccgtcgat ctgcacgcgc gcgttgagcg cctgcaatgt
     5281 cgcggcgtcc agccgctgca tcagtggccg gacgatcgcg ggcagctgcg gatacgccga
     5341 aaccacggcc tggcgggccg tcggcaccgg cgcgtacacc ggctggtcgt ggcggtcgtc
     5401 ctccagcagc acgaggtccg cggcggcgat gccgccgtcg gtgccgtaga ccatcgcggt
     5461 gttgacgtcg ttggtgcgcg cggccgcggc cgcgatcgtc gcggctgtct cgcccccggc
     5521 gagcacgatc ttctggcgtg gctcgaggtg aaagtcgtag gtgcgctcga gcgagcggag
     5581 ggtaccggcg ttggcgaatt cggccgaaca ggcgagcacg gcccggccgc cctcgcggac
     5641 ccagcgtgcg aagtcgctga gcgtcgccag gcggtggcgg cgtgccacgt cacgtcgcac
     5701 ggcgagggcc cacgcgttgc tcgcgggcgc gggcgtgagc cagacgatgc cgtgctgctc
     5761 gcggtcgagt cgcgcaccga gttcgtagcc gcgccgccag tccttccacg cggcgtcgcc
     5821 aggccggttg aagaagaagc ccgcgttgcc ggtgtactcg acgtagaggt cgatctcgcc
     5881 ggccagcagc gcgcggcgca cgagcggcgt gcccccgagt gccgtgcggt cgagggtcgc
     5941 aatgccgtgc gcctcgagca atcgccggat catctggccg atcatgaccg actcgctcga
     6001 gagcttcgac gacaccacga tcggccggga cgcggcggtc gtcgccgcca cgtgcgtacc
     6061 ggcgcacagc cccatggcca gcgtgagcgc cctgagccac tgcgccaacg agttcctccc
     6121 gcgatgcatg cacggatgtg gccgtgccgc acgttggcgg ggcgccgtgg cggcgtcaag
     6181 cctcgccaca ctcgcggctc ggcgcgccgc gagcgtcgac gttcagccga acgcgatcag
     6241 gcggtcgagc tggcggacgt gctcttcctc ccgcgccagg ttccaggcgt acacgtccag
     6301 gacttctggc gtggtcacgg cggtcacctc cgtgagcgcc gcccggtaat ccttgacgcc
     6361 gctttcctct ccttcgcgca aggcgcgcag tgcggccttg tcgcccagca ggctcgccgc
     6421 attgaggacc gtattggccc aggtgcccca cgggccgggg tcgttggccg cggtgcctcc
     6481 cagccggtgc acgagctcgc gcagcttcga cgctgcgagc tcgtggtcgc gcagcatgtc
     6541 cgtcaacttc ccgaaccgcg gatcctcacc gtgcgtcttg cgcacgcgtt cgatggcgtg
     6601 ccggtatgtc tcgatggctg cgagctcatc gcgcagcagt tcgttcaagt cgccgaggat
     6661 gtcagtcgag ctcatggcgc acctcccgtg gccgttcgga ggcttcatca tcttccgcgg
     6721 caacggacaa acaacctgcc gcacaccggc acgcttgtca gcactggctg acgtcggcga
     6781 cgagacgttc gtcacgggat cgatgcgcgg gtcggttccc gggcgccgtc cggcgccggc
     6841 cctcctatac tttgccgggg ccaggcgacc gggctgcggc gatggagcag ggcacatgga
     6901 atccgcggac gagcaccacg tgtcgccgga cgtagcatcg gtgggctcgc tgcactggcg
     6961 atacctcgac cagtacggcg gcgaggtgtt gtgcgccgcc gcggaccggc ggcccgtcgt
     7021 cttccgcacc gtgtacgagg ccgtcgagac ctcgcgacgg ctcggcgggt gttcgctcgg
     7081 ccgctgcctg catgcgccga tgcgctgggt gctgctgcga ccgg
//
LOCUS       DASPFR010000205         7097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_180066, whole genome shotgun sequence.
ACCESSION   DASPFR010000205 DASPFR010000000
VERSION     DASPFR010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180066"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VD737_05255"
     CDS             <1..323
                     /locus_tag="VD737_05255"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10001.1"
                     /translation="FAVDSIPAILAVTRDPFLVFTSNAFAILGLRSLYFAVAGLMALF
                     RYLKYSLVLILAFVGMKMLLVSHYHVPNHISLAIILATLVGGIVASVWVARREAARSE
                     TAGQ"
     gene            complement(358..1809)
                     /locus_tag="VD737_05260"
     CDS             complement(358..1809)
                     /locus_tag="VD737_05260"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXW10002.1"
                     /translation="MPHRLFSTKPIGELHEADTANELHRTLDARALVLLGIGAIIGTG
                     IFVLTGTAAANHAGPALVVSFLIAGLGCALAGLCYAEFASMIPVSGSAYSYSYATLGE
                     GVAWFIGWNLILEYLFAAATVAVGWSGYVVSLVEQFGFHLPDALTHAPFTKGAGHLEI
                     VRTGAIVNLPAVLVIAAITALCYVGIHQSARFNAIVVAIKVTVIVLFIGFGIAAVDAG
                     NWVPYVPENTGKFGEFGWSGVVQAAGIIFFAYIGFDAVSTAAQEAKNPQRDMPIGIMG
                     SLVVCTILYVAVSAVLTGMVHYSELNTPAPVALALDRHPELEWLAGWIKLGAIAGMTS
                     VILVMMLGQPRIFYAMSRDGLLPPFFHKVHPTHRTPYIGTLITGGLAAVIGGLFPVTI
                     LGELVSIGTLLAFTTVCIGVLVLRYTRPDLKRPFRTPAPWVVCLGGAAICTVMMVSLP
                     TDTWLRLGVWTLFGLAIYVFYGFRNSELRKTGR"
     gene            complement(1991..2800)
                     /locus_tag="VD737_05265"
     CDS             complement(1991..2800)
                     /locus_tag="VD737_05265"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXW10003.1"
                     /translation="MTMQPLLNIAVRAARRAGDLIVRNLDRVSTLNVQAKGRNDFVSE
                     IDQAAERDIIETVRRVHPDHAFLGEESGRSGGDSDVIWIIDPLDGTTNFLHGFPQFAV
                     SIGVEVRGRLEHAVVYDPMRQELFTASRGYGAQVDGRRIRVSKQLGLEGALIGTGFPY
                     RANARWLDQYMAMLKSVMQETAGIRRPGAASLDLAYVAAGRLDGFWEIGLSAWDTAAG
                     TLLITEAGGRIGTLTGQPYQQGGHVVAGTPKVFDALVACLGPNVPAELRDV"
     gene            2876..3646
                     /locus_tag="VD737_05270"
     CDS             2876..3646
                     /locus_tag="VD737_05270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HXW10004.1"
                     /translation="MSIRIVLVAPSHPGNIGSAARAMKTMGLDSLWLVAPERFPATEA
                     TVMAAGADDVLQRAHVVSELRDAIADCGLIVGTTARGRHLPWRVVEPRDAALEIAAAA
                     PASEVAILFGSERTGLENSQLQLCHTLLTIPTGTAYTSLNLAMAVQVVSYEVCLALRD
                     RAGGLGSPRSDGPVPLASAADMEKFYAHLEQVLDEIDFRDRTQSGHLMARLRRLFQRT
                     VLDQNEMNILRGILTAVQGRRRRAGDPHVSDAGSQAAP"
     gene            3643..5226
                     /locus_tag="VD737_05275"
     CDS             3643..5226
                     /locus_tag="VD737_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXW10005.1"
                     /translation="MNDRRVVYLDNAATTPVDPRVAAEMRECLGPDGVFGNPSSMSHA
                     YGREAHRRVERAREEVAALVGAQPEQVLFTSGATESDNLAILGAARFQRQRARHVVTS
                     RTEHPAVLDACRQLEREGCSVTYLKPGPGGIVEAEQVAEALQPDTVLVSLMHVNNEIG
                     VVQDVAAVGRLCRDRGVLFHVDAAQGLGKLDLDVHRDSIDLLSLTAHKVHGPKGVGAL
                     CVRREPRIGLVPLQFGGGQERGLRSGTLPTHQVVGMGAACRIARLEMVDDAPRIAGLR
                     SRLWNALKDLPGVLLNGDPVRRVAGILNVSIDGVEGESLLLALCDLAVSSGSACASTH
                     AQPSYVLRALGRSDRLAQSSLRLSLGRFTTEQDVDHAAARIREEVLRLRECRGFGAAT
                     AVTAIDDPRYGVEVQRRLRSLAGAGRLPEVAHKVTGRAGDREQGCSVELEFAVDDRAR
                     ISQGAFLAFGCPHVLAAASWLVERAPGWTRADLEAWDWQEAARALEIPPAKFGRLLTL
                     QDAVRIAARNWASEPGSTV"
     gene            5260..5583
                     /gene="iscA"
                     /locus_tag="VD737_05280"
     CDS             5260..5583
                     /gene="iscA"
                     /locus_tag="VD737_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016537756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HXW10006.1"
                     /translation="MSISLTPSAVERVRTYMASRGKGIGLRLGIKQTGCSGYAYVVSY
                     ADEVGARDVVFDEAGVKVVVDREALKFVDGTEIDFVRTGLNEAFKFHNPNVKGECGCG
                     ESFNV"
     gene            5891..6316
                     /gene="ndk"
                     /locus_tag="VD737_05285"
     CDS             5891..6316
                     /gene="ndk"
                     /locus_tag="VD737_05285"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HXW10007.1"
                     /translation="MALERTLSIVKPDGVKKNVIGDVYQRFEKAGLRIVAARMLCLSQ
                     AHAEAFYGIHRERPFFRDLVKYMTSGPVVVQVLEGEDAVAKNRDIMGATDPKKAAPGT
                     IRADLADSIEANVVHGSDSLENAAREIAFFFSETELCPR"
     gene            6357..>7097
                     /gene="rlmN"
                     /locus_tag="VD737_05290"
     CDS             6357..>7097
                     /gene="rlmN"
                     /locus_tag="VD737_05290"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HXW10008.1"
                     /translation="MTSEATTPAPERTNLLGLTRSGLEAFVVAMGEKPFRARQLFKWI
                     YKRAEGDIAAMTDLGKDFRRRLEEIAEIRTPDILGVQVSADGTRKWALRFEGGQAIEM
                     VFIPEPGRGTLCISSQVGCQMDCQFCSTGAQGFNRNLTTAEIVGQVWLANRELGYAPD
                     GDRVITNVVFMGMGEPLANYRNVVPAADVMMDDLGLDLSRRRVTVSTSGLVPQMYRIA
                     DETNVALAVSLHAPNDELRSQIVPINRRH"
BASE COUNT         1139 a         2403 c         2470 g         1085 t
ORIGIN      
        1 tgttcgcggt cgactccatc ccggcgatcc tcgcggtcac gcgcgacccg ttcctggtct
       61 tcacgtccaa cgcattcgcg atcctcgggc tacgatcgct ctacttcgcc gtggcgggcc
      121 tcatggcgct gttccgctac ctcaagtaca gcctggtgct gatcctggcg ttcgtcggca
      181 tgaagatgct gctcgtgagc cactaccacg tgcccaacca catctcgctc gccatcatcc
      241 tggcgacgct cgtcggtggc atcgtcgcct cggtctgggt cgcgcgccgc gaggcggcgc
      301 gcagcgaaac ggccggccag tagacaggcg ccgcggcggc gcggcgtcgg cgcccggtca
      361 gcgtccggtc ttgcgcagct cgctgttgcg aaagccgtag aagacgtaga tcgccaggcc
      421 gaacagcgtc cacacgccga gccgcagcca cgtatcggtc ggcagcgaga ccatcatgac
      481 cgtgcagatc gccgcgccgc cgaggcacac cacccacggc gcgggcgtgc ggaacggtcg
      541 cttgaggtcc gggcgcgtgt agcgcagcac gagcacgccg atgcagacgg tcgtgaaggc
      601 gagcagcgtg ccgatcgaga cgagctcgcc caggatggtg acgggaaaca ggccgccgat
      661 cacggccgcg agcccgcccg tgatcagcgt gccgatgtac ggcgtcctgt gcgtcgggtg
      721 caccttgtgg aagaacggcg gcagcaggcc gtctcgcgac atcgcgtaga agatgcgcgg
      781 ctgcccgagc atcatcacga gaatcacgga cgtcatgccg gcgatcgcgc cgagcttgat
      841 ccagcccgcg agccactcga gctcggggtg ccggtcgagc gcaagcgcga ccggggccgg
      901 cgtgttgagc tcgctgtagt gcaccatgcc cgtcagcacc gcggacaccg cgacgtagag
      961 gatcgtgcag acgacgaggc tgcccatgat cccgatcggc atgtcgcgct gcgggttctt
     1021 ggcctcctgc gccgcggtcg agaccgcgtc gaagccgatg taggcaaaga agatgatgcc
     1081 ggccgcctgc acgacgccgc tccagccgaa ctcgccgaac ttgccggtgt tctcggggac
     1141 gtacgggacc cagttgccgg cgtccacggc agcgatcccg aatccgatga acagcacgat
     1201 caccgtgacc ttgatcgcga cgacgatggc gttgaagcgc gccgactggt ggatgccgac
     1261 gtagcagagc gcggtgatcg ccgcgatgac cagaaccgcc ggcaggttga cgatcgcccc
     1321 cgtgcgcacg atttccaggt ggccggcgcc cttcgtgaag ggcgcgtgcg tcagcgcgtc
     1381 cggcaggtgg aaaccgaact gctcgacgag gctcacgacg tagcctgacc agccgacggc
     1441 gacggtggcc gcggcgaaca ggtattccag gatcaggttc cagccgatga accacgcgac
     1501 gccctcgccg agcgtcgcgt acgaatagga gtacgcgctg cccgacaccg ggatcatcga
     1561 cgcgaattcg gcgtagcaga gaccggccag cgcgcagccc aggcccgcga tcaggaacga
     1621 gacgacgagc gcgggaccgg cgtggttcgc cgccgccgtg ccggtcagca cgaagatccc
     1681 ggtgccgatg atcgcgccga tgccgagcag cacgagcgcg cgtgcgtcga gggtgcggtg
     1741 gagttcgttg gcggtgtcgg cttcgtgcag ttcgccgatg ggcttggtcg agaacaggcg
     1801 atgcggcatg ggtgcgcgtg gtcctgggcc cggcgggcaa tgccgcggga ctctaccgga
     1861 cgtggccggg ccgggatagg gcccgccgcc cgccccgaaa atggggacac tcctgattaa
     1921 cgaccgtaaa tggggacact cctgattatg tggaaaatca ggaatgtccc catttacggt
     1981 gtcgccgtcc tcagacgtcg cgcaactcgg cgggcacgtt cgggccgagg cacgcgacca
     2041 gcgcgtcgaa caccttgggc gtgccggcga ccacgtgacc gccctgctgg tacggctgcc
     2101 ccgtcagcgt gccgatgcgt ccgccggctt cggtgatcaa cagcgtgccg gcggcggtat
     2161 cccacgcact caggccgatc tcccagaaac cgtcgaggcg gccggcggcg acgtacgcga
     2221 ggtcgagcga cgccgcgccc gggcgccgga tgcccgccgt ctcctgcatc accgacttga
     2281 gcatcgccat gtactggtcc agccagcgtg cattggcccg ataggggaag ccggtgccga
     2341 tcagcgcgcc ctcgaggccg agttgcttgc tgacgcggat gcgacgaccg tccacctgcg
     2401 cgccgtaccc gcgcgacgcg gtgaagagtt cctgccgcat cgggtcgtag accacggcgt
     2461 gctcgagccg cccgcgcacc tcgacgccga tcgacaccgc gaactgcgga aagccgtgca
     2521 ggaagttggt cgtgccgtcg agcgggtcga tgatccagat gacgtcgctg tcgccgccgc
     2581 tgcgtcccga ttcctcgccg aggaacgcgt ggtcggggtg gacgcgacgc accgtctcga
     2641 tgatgtcgcg ctcggctgcc tggtcgatct cggagacgaa atcgttgcga cccttcgcct
     2701 gcacgttgag cgtcgacacc cggtcgaggt tccgcacgat caggtcgccg gcgcgacgcg
     2761 ccgcgcggac ggcgatgttg agcaaaggct gcatggtcat gcggcgggat ctcgacgtcg
     2821 gtggtggcaa agagcggcgg ggaacccgcc tcgaagcccg ctagaatacc agagcatgtc
     2881 catccgcatc gtcctggtcg cgccttcgca cccgggcaac atcggctccg cggcgcgcgc
     2941 catgaagacg atggggctcg attcgctctg gctcgttgca ccggaacgct ttccggccac
     3001 cgaggcgacc gtgatggccg ccggcgccga cgacgtcctg cagcgtgcgc acgtcgtgag
     3061 cgagcttcgc gatgccatcg ccgactgcgg gctcatcgtc ggcacgaccg cgcgcggacg
     3121 tcaccttccc tggcgcgtcg tcgagccgcg cgatgcggcg ctcgagatcg cggcggcggc
     3181 acccgcgagc gaagtggcga tcctgttcgg cagcgagcgg accgggctcg agaactcgca
     3241 gctgcagctg tgccacacgc tgctgacgat tccgacgggc accgcataca cctcgctcaa
     3301 cctcgcgatg gccgtacagg tcgtgagtta cgaagtctgc ctggcgctgc gtgatcgggc
     3361 gggcggcctc ggctcgccgc gcagcgacgg gccggtgccg ctcgcgagcg ctgcggacat
     3421 ggagaagttc tacgcgcacc tcgagcaggt gctcgacgaa atcgactttc gcgaccgcac
     3481 ccagtcgggc cacctgatgg cacgtctgcg tcggctgttc cagcgcaccg tgctcgacca
     3541 gaacgaaatg aacatcctgc gcggcatcct gacggcggtg cagggaagac gacgacgcgc
     3601 gggcgatcct cacgtgtcgg acgccggttc gcaggccgca ccgtgaacga ccgacgcgtc
     3661 gtctatctgg acaatgccgc gacgaccccg gtcgacccgc gcgtcgctgc ggagatgcgc
     3721 gaatgcctgg gaccggacgg cgtcttcggc aatccgtcgt cgatgtcgca cgcgtacggt
     3781 cgcgaagcgc atcgacgcgt cgagcgcgcc cgggaggaag tggccgcgct ggtgggcgcg
     3841 cagccggagc aggtgctgtt cacgtccggc gcgaccgaat ctgacaacct cgcgatcctc
     3901 ggcgccgcgc gcttccagcg gcagcgcgcg cggcacgtcg tgacctcgcg cacggaacat
     3961 ccggcggtgc tcgacgcctg ccgccagctc gagcgcgaag gctgcagcgt cacctacctc
     4021 aagccggggc cggggggcat cgtcgaagcg gagcaggtgg ccgaagcgct gcagcccgac
     4081 acggtgctgg tgtcgctgat gcacgtgaac aacgagatcg gcgtcgtgca ggacgtggcg
     4141 gcggtggggc ggctctgccg cgaccgaggc gtcctgtttc acgtcgacgc cgcgcagggc
     4201 ctcggcaagc tggatctcga cgtccatcgc gacagcatcg acctgctgtc actgacggcg
     4261 cacaaggtgc acgggccgaa gggggtgggc gcgctctgcg tccggcgtga gccgcgcatc
     4321 ggcctggtgc cgctgcagtt cggcggtggc caggagcgtg gcctgcgctc gggcacgttg
     4381 ccgacgcacc aggtggtcgg catgggcgcc gcgtgccgga tcgcgcgcct cgagatggtc
     4441 gacgacgcgc cgcgcatcgc gggcttgcgc agccgactct ggaatgcgct caaagacctg
     4501 ccgggcgtgc tgctgaacgg cgacccggtg cggcgcgtgg ccggcatcct caatgtctcg
     4561 atcgacggcg tcgagggcga gtcgctgctg ctcgcgctct gcgacctcgc ggtttcgtcg
     4621 gggtcggcct gtgccagcac gcacgcgcag ccgtcgtacg tgctgcgtgc gctcggtcgc
     4681 agcgaccggc tcgcgcagag ttcgctgcgg ctgagcctcg gccggttcac gacggagcag
     4741 gacgtcgatc acgcggccgc gcgcatccgc gaagaggtgc tgcgcttgcg cgaatgccgt
     4801 gggttcggtg ctgccacagc cgtgacggcg atcgacgatc cgcgttatgg ggtggaggtg
     4861 cagcggcggc tccgttcgct ggcgggggcc ggccgcctgc cggaggtcgc gcacaaggtc
     4921 acgggccgcg cgggggaccg cgaacagggc tgctccgtcg agctcgagtt cgcggtcgac
     4981 gaccgggccc ggatctcgca gggcgcgttc ctggcgtttg gctgtccgca cgtgctcgct
     5041 gcggcgtcct ggctggtcga acgggcgcct ggctggaccc gcgccgacct cgaggcctgg
     5101 gactggcagg aggcggcgcg cgcgctcgag attccgccgg ccaaattcgg gcgcctgctc
     5161 acgcttcagg acgcggtgcg catcgcggcc cggaactggg ccagcgagcc ggggtctaca
     5221 gtgtagaatt ttgcaggcac ctctggagga ccgcaccgca tgtccatctc gctcaccccg
     5281 tccgctgtcg agcgtgtccg cacgtacatg gcgagccgcg gcaagggcat cggtctgcgt
     5341 ctgggcatca agcagaccgg ctgctccggc tacgcctacg tggtgagcta cgcggacgag
     5401 gtcggcgccc gcgacgtggt gttcgacgaa gcaggggtca aggtggtggt cgaccgcgag
     5461 gcgctcaagt tcgtcgacgg gaccgagatc gacttcgtcc gcacgggcct gaacgaggcc
     5521 ttcaagttcc acaatcccaa cgtcaagggc gagtgtggct gcggcgaaag cttcaacgtg
     5581 taagaagtgg ctagtggtca gtggctagtg gctagtgcga acacccccaa cccctggcgc
     5641 ttaccttagc gagccactga ctactagcca ccagccacta gccactagcc actagccact
     5701 cgcactaggc ctaactgccc gtccgggcag tgatttcttg gccggcagtg cccgtcccgg
     5761 tatacttcgc cgttaacttt tcgtggccgg tacccccacc ggcccgcgtt gctgcgggcg
     5821 gggccaggtg ccgggtcatc ttccagcggt ggacccgatc cgccgccgtc acccagtcca
     5881 aggatccgca atggccctcg aacgcaccct ttccatcgtc aagccggatg gagtcaagaa
     5941 gaacgtaatc ggcgacgtgt accagcgctt cgagaaggcg gggttgcgca tcgtcgcggc
     6001 gcggatgctc tgcctgagcc aggcgcacgc cgaggctttc tacggcatac atcgcgagcg
     6061 gccgttcttc cgggacctcg tcaagtacat gacctcgggc ccggtggtgg tgcaggtgct
     6121 cgagggcgag gacgccgtcg cgaagaaccg cgacatcatg ggcgcgaccg atccgaaaaa
     6181 ggccgcgccg ggcacgatcc gcgcggacct ggccgacagc atcgaggcca acgtcgtgca
     6241 tggctcggac agcctcgaaa acgcggcccg cgaaatcgcg ttcttcttct ccgaaacgga
     6301 gctgtgtccg cgctgagcgc aacgcactcc gcccgcaccc agcggtccaa accggcatga
     6361 cgagcgaagc gacgacgcct gcacctgagc gcacgaacct cctgggcctc acgcggtccg
     6421 ggctggaggc gttcgtcgtc gcgatgggcg agaagccgtt ccgggcccgc cagctgttca
     6481 agtggatcta caagcgtgcc gaaggcgaca tcgccgcgat gacggacctc ggcaaggact
     6541 tccgtcgtcg cctcgaggag atcgcggaga tccgcacgcc cgacatcctc ggcgtgcagg
     6601 tgtccgccga cggcacccgg aagtgggcgc tgcggttcga gggcggccag gccatcgaga
     6661 tggtgttcat acccgagccg ggtcgcggca cgctctgcat ctcgagccag gtcggctgcc
     6721 agatggactg ccagttctgc tcgaccggcg cccagggctt caaccgcaac ctgaccacgg
     6781 ccgagatcgt gggccaggtc tggctcgcca atcgcgagct cggctatgcg cccgacggcg
     6841 atcgcgtgat caccaacgtc gtgttcatgg gcatgggtga gccgctcgcc aactaccgca
     6901 acgtggttcc ggccgccgac gtcatgatgg acgacctcgg gctcgacctc tcgcgccgcc
     6961 gcgtcaccgt gagcacctcg ggcctcgtgc cgcagatgta ccgcatcgcc gacgaaacca
     7021 acgtggcgct cgcggtctcg ctgcacgcgc ccaacgacga gctgcgcagc cagatcgtgc
     7081 cgattaaccg gcggcat
//
LOCUS       DASPFR010000206         3179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1767276, whole genome shotgun sequence.
ACCESSION   DASPFR010000206 DASPFR010000000
VERSION     DASPFR010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767276"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="VD737_05295"
     CDS             <1..617
                     /locus_tag="VD737_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXW10009.1"
                     /translation="PRLRELCTKHGILLIFDEVITGFGRLGAPFGAQYFGVTPDMITM
                     AKGLTNGAVPMGAVAASAQVHDTFMQGPEGAIELFHGYTYSAHPVACAAGIATQEIYR
                     REGLLTRAAELSDTWADGVHSLRGLPHVIDLRNLGLIGAIELEPIAGKPGLRGYQTFV
                     RAFEKGLLVRVTGDIIALSPPLIIEKAHIEQVFGLLGETLKESG"
     gene            complement(781..1488)
                     /locus_tag="VD737_05300"
     CDS             complement(781..1488)
                     /locus_tag="VD737_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HXW10010.1"
                     /translation="MKLTQGLAAVGLSLAAMAASAEVTGTVGVYSDYNWRGITQTSQD
                     PALQGSIDYAHDSGFYAGVWASNVDFGDCCDENIETDIYLGFSGGETVTWDFGGVYYL
                     YPGAEDIDFWEIYAGLGYNWLSGKLSYSSDFANYDETALYFELNGEWELPANFGLNAH
                     VGYSDGDGIKLAYGEDSYFDWAVGVSYTLGHFDLGLKYADGSDLEALDGTPGDVGSSE
                     GVAIFSISTTFPWSAEE"
     gene            complement(1714..2091)
                     /locus_tag="VD737_05305"
     CDS             complement(1714..2091)
                     /locus_tag="VD737_05305"
                     /inference="COORDINATES: protein motif:HMM:NF017690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXW10011.1"
                     /translation="MTVTLGPEVVKIDGNAAAEESLDLDPSRLAPGSPVPAQFVRNAF
                     TDATGRFFGGIWRSSEGAWRVSYTENELCVLTGGRVRVSDDAGRSWTFRPGDCFVMPA
                     GFVGLWEVLEPAEKFYAIYEPPA"
     gene            complement(2137..2811)
                     /locus_tag="VD737_05310"
     CDS             complement(2137..2811)
                     /locus_tag="VD737_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HXW10012.1"
                     /translation="MRRFVDRAEAGRQLAAALVGAVPGDTGVVLGLPRGGVPVAAEVA
                     RTLGLPLDVLCVRKLGVPLHPELAMGAIASGGAVVRNDDVLSTLSSPDAEFERVLERE
                     RVELARRERCFRGDAPALDVRDRGVILVDDGLATGATMAAAVRALRSLAPAMIVAAAP
                     VGSREAFARIEELADRVVCLNAPTFFGSVGSFYEAFDQTNDDEVRELLALARRRVAGD
                     SGETGA"
     gene            complement(2815..>3179)
                     /locus_tag="VD737_05315"
     CDS             complement(2815..>3179)
                     /locus_tag="VD737_05315"
                     /inference="COORDINATES: protein motif:HMM:NF025923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10013.1"
                     /translation="LVGPVAGRHVVIVDDLIGAGTTIMRATHACRAAGALSVTAFATH
                     GVFAPGAERLLGPDGPDRLLVTDTILPVRLNTGAGERGTLEVLPASALFAEAVRRIEA
                     GESVVALREMERKPPPRE"
BASE COUNT          522 a         1098 c         1068 g          491 t
ORIGIN      
        1 tgccgcggct gcgcgaactc tgcaccaagc acggcatcct gctgatcttc gacgaagtca
       61 tcaccgggtt cggccgtctc ggcgcgccgt tcggcgcgca atatttcggc gtgacgcccg
      121 acatgatcac gatggccaag ggtctcacca atggcgccgt gccgatgggc gccgtcgccg
      181 cgagcgcgca ggtccacgat acgttcatgc agggccccga aggcgcgatc gagctgttcc
      241 acggctacac gtactccgcg cacccggtcg cctgtgcggc gggcatcgcg acccaggaga
      301 tctatcgtcg cgagggcctg ctcacgcgcg ccgccgagct gtcggacacc tgggccgacg
      361 gcgtgcactc actgcgcggc ctgccgcacg tgatcgacct gcgcaacctg gggctgatcg
      421 gcgcgatcga actcgagccg atcgccggca agccgggcct gcggggctac cagacgttcg
      481 tccgtgcgtt cgagaaaggc ctgctcgtgc gcgtcaccgg cgacatcatc gcgctgtcac
      541 cgccgctgat catcgaaaag gcgcacatcg aacaggtgtt cgggctgctg ggcgaaaccc
      601 tgaaggaaag tggctagtgg tcagtggcta gtggtcagtg gttagtgaag aaccgactgc
      661 gtcggtccgc ggcgcagccg ctccgcacta gccactagcc actagccact gacgacggcg
      721 cccggcctcc tcgcggagac cgggcgtcga tgcacgaaga acgttcgtgc aggttgcttg
      781 ttattcttcg gccgaccagg ggaacgtggt cgagatcgag aagatcgcca cgccttcgct
      841 gctgccgacg tcgcccggcg tgccgtcgag tgcctcgagg tcgctgccgt cggcgtactt
      901 caagccgagg tcgaagtgac ccagcgtgta cgacacgccc acggcccagt cgaagtagct
      961 gtcttcgccg tacgccagct tgatgccgtc accgtcgctg tagccgacgt gcgcattgag
     1021 gccgaagttg gccggcaatt cccattcgcc attcagttcg aagtacaacg cggtttcgtc
     1081 gtagttggcg aagtcgctcg agtacgacag cttgccgctc agccagttgt acccgaggcc
     1141 ggcgtagatc tcccagaagt cgatgtcttc ggcacccggg tacaggtagt agacgccgcc
     1201 gaagtcccac gtcaccgtct cgccgccgct gaagcccagg taaatgtcgg tctcgatgtt
     1261 ttcgtcgcag cagtcgccga aatcgacgtt gctcgcccag acgccggcgt agaagccgct
     1321 gtcgtgggcg tagtcgatgc tgccctgcaa ggcagggtcc tgcgacgtct gcgtgatgcc
     1381 gcgccagttg tagtcgctgt acacgccgac cgtgccggtc acttccgcac tggcagccat
     1441 cgctgcgagg ctcaacccga ctgcagccag cccttgggtc aatttcatcg gatacccctt
     1501 tgttgtctca acgcaacata taaacgactg acgacaccgt gaattgtagc agaggcgaaa
     1561 cggtcttcag gcgctgcgcc cgcgcacggc ggttcgcgac ggtgcggtgc aggaagcgtg
     1621 ccagtcgcaa agcgagtttt ctgcttggac gacccgtcgt acccgcaccg tttgggcgca
     1681 ttgcaccaag gccggcgcac cgtcaaggat cactcaggcc ggcggctcgt agatcgcgta
     1741 gaacttttcg gccggctcga gcacttccca cagccccacg aatccggccg gcatcacgaa
     1801 gcagtcgccc gggcgaaagg tccagctgcg tccggcgtcg tcactgactc ggacgcggcc
     1861 cccggtcagc acgcagagct cgttctcggt gtacgagact cgccaggcgc cttcgctgct
     1921 gcgccagatg ccgccgaaga agcgcccggt ggcgtcggtg aacgcattgc gcacgaactg
     1981 cgccggaacg ggactgcccg gcgccaggcg cgacggatcg aggtcgagcg actcctcggc
     2041 ggcggcgttg ccgtcgatct tgaccacttc cggacccagc gtgacggtca tgcgcgatgc
     2101 tccgaggcag ccgcacgcgc ggccgcggcg agggtatcac gcgcccgtct cgccgctgtc
     2161 gcccgcgaca cgccggcgtg ccagggccag cagctcgcgg acttcgtcgt cgttcgtctg
     2221 gtcgaacgcc tcgtagaacg agccgacgga accgaagaac gtcggtgcgt tcaggcacac
     2281 gacccgatcg gcgagttcct cgatgcgcgc gaacgcctcg cgggaaccga ccggcgcggc
     2341 cgcgacgatc atggcgggtg cgagcgaccg caatgcccgt acggccgccg ccatggtggc
     2401 gccggtggcg agaccgtcgt cgacgaggat cacgccgcgg tcacgcacgt cgagcgccgg
     2461 cgcgtcgccg cgaaagcagc gctcgcgacg cgcgagctcg acacgctccc gctcgagcac
     2521 gcgctcgaac tcggcgtccg gcgacgacaa cgtggagagg acgtcgtcgt tgcgaacgac
     2581 ggcgccgccg ctggcgatgg cgcccatggc gagctcggga tggagcggga cgccgagctt
     2641 gcggacgcac agcacgtcga gcggcaggcc gagcgtcctg gcgacctcgg ccgccaccgg
     2701 cacgccgccg cgcggcaacc cgagcaccac gccggtgtcc cccggcaccg caccgacgag
     2761 tgccgcggcg agctggcgtc ccgcctcggc gcggtcgacg aacctgcgca tcgttcactc
     2821 ccggggcggc ggcttgcgct ccatttcgcg aagtgcgacg accgactcgc cggcctcgat
     2881 gcgacgcacg gcttcggcga acagcgccga tgccgggagc acttcgagcg tgccccgttc
     2941 gcccgcgccc gtgttcaggc gcaccggcag gatggtgtcg gtgacgagca gccggtcggg
     3001 gccgtcggga ccgagcagtc gctcggcgcc cggcgcgaac acgccgtggg tcgcgaaggc
     3061 cgtcacggag agtgcgccgg ccgcgcgaca agcatgggtc gcgcgcatga tcgtggtgcc
     3121 ggcgccgatc aggtcatcca cgatcacgac gtgacgcccc gccacggggc cgacgagca
//
LOCUS       DASPFR010000207         4956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_425075, whole genome shotgun sequence.
ACCESSION   DASPFR010000207 DASPFR010000000
VERSION     DASPFR010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425075"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VD737_05320"
     CDS             <1..879
                     /locus_tag="VD737_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10014.1"
                     /translation="FPMFDDRRRSAAKVSPLYDWRPMSTYIRRPPYWEGALAGERGLR
                     RMRPLAVLGDNITTDHLSPSNAILRDSAAGEYLAKMGLPEEDFNSYATHRGDHLTAQR
                     ATFANPQLVNEMAVTDGTVRKGSLARLEPDGVVMRMWEAIETYMHRKQPLIIVAGADY
                     GQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLEFKPGVDRKTLGLDGS
                     ETYDVVGHRTPRADLTLVIHRRSGERVEVPVTCRLDTAEEVSIYEAGGVLQRFAKDFL
                     EATSPALAGEAGRQAV"
     gene            963..2150
                     /gene="prpF"
                     /locus_tag="VD737_05325"
     CDS             963..2150
                     /gene="prpF"
                     /locus_tag="VD737_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004783077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HXW10015.1"
                     /translation="MPSAPQIRIPATYMRGGTSKGVFFRLDDLPVRAQVPGEARDRLF
                     MRVIGSPDPYAAHIDGMGGATSSTSKCVIVSKSSRAGHDVDYLYGQVSIDKAFVDWSG
                     NCGNLSAAVGPFAISNGLVDPGRVPRDGTCTVRIWQANIAKTIVAHVPMTDGVVQETG
                     EFELDGVTFPAAEIVLEFVDPSDDGEAGAAMFPTGQLVDDLEVPGVGTFKATLISAGI
                     PTVFVNAADIGYTGTELREAINGDSRQLERFEKIRVAGALRMGLIETPEEALKRQHTP
                     KVAFVAPSRSYVSSSGKEISANEIDLLVRALSMGKLHHAMMGTAAVAIAAAAAVPGTL
                     VNLAAGGGQRQAVRFGHPSGTLRVGAEASQVNRQWTVTKAIMSRSARVLMEGSVRVPA
                     DAF"
     gene            2435..4690
                     /locus_tag="VD737_05330"
     CDS             2435..4690
                     /locus_tag="VD737_05330"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CFI-box-CTERM domain-containing protein"
                     /protein_id="HXW10016.1"
                     /translation="MNTWRSVLVALAMSGVVSSCGGGGDGDGDGHGRGPPPDLAGVWA
                     GNWQGADPAIGEVSGVWQATVSPAGKDGVSGSGFLTGDVDCMDGRLSGSAGETVYRGT
                     VDRSPCMLNEWQLRALSTQDQMASGTWTQRTTNAQGTFSGVRIANVGGPRIERMHPAS
                     AAPGAIVTLVGSGFDPIAANNSVRFGDASIPTPASAASATTLTVRVPDPAYTAPVAVT
                     TASGRALSPRAFVADPSTPAPLVSGTVATSAMPRALAFSPDGRKLYMVNAGSVGLLTT
                     QSNTTLVPTAGLPSKVSAAPLGIAAAADGRRVYVAAGNNGVVAADAALMQPVADEAIA
                     GFATATDAPAGTAALALSPDGTRLYVADNLPGGVLRIVNLGSRTVVTSTPFGASLRPT
                     CVAASPDGTRVLLGVRDAAGTQSDFVAVLDPLTGQVDGTTIPLGLQASPVAIAYSQDG
                     TRAYVSNRGLGTVSVIDTVSRAVASTLGGLQSPAGLALAPDGRTLLVANEGDDSVVVV
                     DLSTQQREAIPIVLPGVSRAAPVAVAVSPDGTQAYVALGATNAIAELGNSAPLEIRIG
                     GYGIGTVLSSPAGVQCGTACRARFEVGTRVALTALPGFGSQFVGWSGNGCGSGSVTVA
                     RPAVTCTANFDNVSPSTGSAGGSGCFVATAAYGSAMAPQVVALREFRDRYLLANAPGR
                     AFVRWYYQWSPGAAHVIRGDETLRKLARAALWPAVAAVTHPKLAWAASALLLWLAVEL
                     AVHMSRARTAR"
     gene            4731..>4956
                     /locus_tag="VD737_05335"
     CDS             4731..>4956
                     /locus_tag="VD737_05335"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HXW10017.1"
                     /translation="MSDVPTKSAARPEPDRLLVEIADYAMNYDGGSPLARETAYHCLL
                     DSLACALQALKYPACTKLLGPVVPGATMARG"
BASE COUNT          765 a         1732 c         1727 g          732 t
ORIGIN      
        1 ttcccgatgt tcgacgaccg gcgtcgcagc gcggcaaagg tgagcccgct ctacgactgg
       61 cgaccgatgt cgacgtacat ccgtcgcccg ccatactggg aaggcgcgct ggccggcgaa
      121 cgcgggcttc gccgcatgcg gccgctcgcg gtgctcggtg acaacatcac gacggaccac
      181 ctgtcgccgt cgaacgcgat cctgcgcgac agcgccgccg gcgagtacct tgcgaagatg
      241 ggactgccgg aagaggactt caactcgtac gcgacgcatc gcggcgatca cctgacggcg
      301 caacgcgcga ccttcgcgaa cccgcagctc gtcaacgaga tggccgtgac cgacggcacg
      361 gtgcgcaagg gatcgctcgc gcgcctcgag cccgacggcg tcgtgatgcg catgtgggaa
      421 gcgatcgaga cctacatgca ccgcaagcag ccgttgatca tcgtcgccgg tgcggactac
      481 ggccagggat cgtcgcgcga ttgggccgcc aagggcgtac ggctcgccgg ggtcgaggcc
      541 atcgtcgccg aagggttcga gcgaatccac cgcacgaacc tgatcggcat gggcgtgctg
      601 ccactcgagt tcaagccggg tgtcgatcgc aagacgctgg gactggacgg tagcgagacg
      661 tacgacgtcg tcggccaccg cacaccgcgt gcggacctga cgctcgtgat ccatcgacgc
      721 agcggcgagc gcgtggaggt tcctgtcacg tgccggctcg acaccgccga ggaagtctcg
      781 atctacgagg ccggtggagt gctgcagcgg ttcgcgaagg acttcctgga agccacctcg
      841 cccgcgttgg cgggagaggc cggccgtcag gccgtgtgag ggcttcttac ctctcccggc
      901 tttgcgggag aggtcgcgcg cagcgcgggt gagggctgtt actacttcag gacaacgctc
      961 tgatgccatc ggcaccgcag atccgtatcc ccgccaccta catgcgcggc ggcaccagta
     1021 aaggcgtctt cttccgcctg gacgacctgc ctgtacgggc gcaagtgccg ggcgaagcac
     1081 gcgaccggct gttcatgcgc gtgatcggca gcccggaccc gtacgccgcc catatcgacg
     1141 gcatgggcgg cgcgacctcg agcaccagca agtgcgtgat cgtgtcgaag agctctcggg
     1201 cgggtcacga cgtcgactac ctctacggcc aggtttcgat cgacaaggcc ttcgtcgact
     1261 ggagcggcaa ctgcggcaac ctctccgccg cggtcgggcc gttcgcgatc tcgaacggcc
     1321 tcgtcgaccc gggccgcgta ccgcgtgacg gcacctgcac cgtgcggatc tggcaggcca
     1381 acatcgccaa gaccatcgtc gcgcacgtgc cgatgaccga cggcgtcgtg caggaaaccg
     1441 gcgaattcga gctcgacggc gtgacgtttc cggccgccga gatcgtgctc gagttcgtcg
     1501 acccttccga cgacggcgag gcgggcgccg cgatgttccc gaccggacag ctcgtcgacg
     1561 acctcgaggt gcccggcgtc ggaacgttca aggcgacatt gatcagcgcc ggcatcccga
     1621 cggtcttcgt gaacgcggca gacatcggct acacgggcac cgagttgcgc gaagcgatca
     1681 acggggactc gaggcagctg gagcgcttcg aaaagattcg cgtggccggt gcgctgcgca
     1741 tgggactgat cgagacgccc gaggaagcgc tcaaacggca gcacacgcca aaggtggcgt
     1801 tcgtcgcacc ctcgcgcagc tatgtctcgt cgagcggcaa ggagatctct gcgaacgaga
     1861 tcgacttgct cgtgcgcgcg ctgtcgatgg gcaagctgca tcacgccatg atgggcaccg
     1921 ccgccgtcgc gatcgccgcg gcagcggccg ttccgggcac gctcgtgaac ctcgccgccg
     1981 gtggtggcca acgtcaggcg gtgcggttcg gtcatccgtc gggcacgttg cgcgtcggcg
     2041 ccgaagccag ccaggtcaat cggcagtgga ccgtgaccaa ggcgatcatg agtcgcagcg
     2101 cccgcgtgct gatggaaggc tcggtgcgcg tgcccgcgga cgccttctga cccccatctc
     2161 tcccgccatc gcgggagagg tcggccggcg gccgggcgag ggcctcctgc agagcctggc
     2221 ccgcgctgac cgccacgccg ggatgaagag accgtccagc ggccctggcc ggattgccgg
     2281 ttctgcacga cagccctcgg caagccgacg acgccaagaa gtacgcattg attcccaagc
     2341 gctcggcgcg acactggcaa caggactgcg ctgaccgcgg taccgagacc cgaccgatcc
     2401 cggccgtccg agcccgcgga accgagggtg ccccatgaac acctggcgct cagtactcgt
     2461 cgccctggcg atgagcggtg tcgtttcgtc ctgtggcggt ggcggtgatg gagatggcga
     2521 cggccatggt cgcggaccgc cgcccgacct cgccggcgtc tgggcaggca actggcaggg
     2581 cgccgacccc gcgatcggtg aagtgtccgg cgtgtggcag gcgaccgtca gtccggcggg
     2641 caaggacggc gtctcgggca gcggcttcct caccggggac gtcgattgca tggacgggcg
     2701 gttgtccggg tccgcgggcg agaccgtcta caggggcacg gtcgaccgct caccgtgcat
     2761 gctcaacgag tggcagctgc gtgcgctcag tacgcaggac cagatggcgt cgggcacctg
     2821 gacgcagcgc accacgaacg cccagggcac gttctccggc gtgcgcatcg cgaatgtcgg
     2881 cggcccgcgc atcgagcgca tgcacccggc ctccgccgcg cccggtgcca tcgtcacgct
     2941 cgtcggttcc gggttcgacc ccatcgcggc caacaacagc gtgcgcttcg gcgatgcgtc
     3001 gatacccaca ccggcgtcgg ccgcttcggc caccacgctg acggtccgag tgcccgaccc
     3061 ggcgtacacc gcaccggtcg cggtgacgac ggcttcgggc cgcgccctca gcccgcgcgc
     3121 gtttgtcgcg gacccatcca cgccagcgcc gctcgtgtcg ggaaccgtcg cgacctccgc
     3181 gatgccgcgg gcactggcct tcagcccgga cggtcgcaag ctatacatgg tgaacgcggg
     3241 ctccgtcggc ctgctgacca cgcagtccaa cacgacgctc gtcccgacag cgggtttacc
     3301 cagcaaggtc agcgccgcac cgctcggcat cgctgcggcc gcggacggcc gccgtgtcta
     3361 cgtcgctgcg ggcaacaacg gtgtcgtggc cgcggacgcg gcgctgatgc agccggtcgc
     3421 agacgaagcg atcgccggat ttgcgacggc gaccgacgcg ccggccggca ccgccgcgct
     3481 tgccttgagc ccggacggaa cccgcctcta cgtcgcggac aacctgccgg gcggcgtcct
     3541 gcgcatcgtc aacctgggct cgcgcacggt cgtgacgtcg acgccgttcg gcgccagcct
     3601 gcggccgacg tgtgtcgccg cgagcccgga cggcacgcgg gtgctgctgg gggtgcgcga
     3661 cgcggctgga acgcagagcg acttcgttgc cgtgctcgat ccgctgacgg gacaggtcga
     3721 cgggacgacg atcccgctcg gcctgcaggc atcgccggtc gcgatcgcct actcgcagga
     3781 tggcacgcgg gcctacgtct cgaaccgggg cctcggcacg gtgtcggtca tcgacacggt
     3841 gagccgcgcc gtggcgtcga cgctcggcgg attgcagtct ccggccggcc ttgcgctcgc
     3901 accggacggc aggaccctgc tcgtcgcgaa cgaaggcgac gacagcgtcg tggtcgtcga
     3961 tctctcaacg cagcagcgcg aggcgatccc catcgtgctg ccgggcgtct cgagggctgc
     4021 tccggtcgcg gtcgcggtga gcccggacgg cacgcaagcg tacgtcgccc tcggcgcgac
     4081 caacgcgatc gcggaactcg gcaactcggc gccgctggag atccggatcg gcggctatgg
     4141 catcggcacg gtgctctcat cgccagccgg cgtacagtgc ggaaccgcct gccgcgcccg
     4201 cttcgaggtc ggcacccgcg tcgccctgac ggcgctgccg ggcttcggct cgcagttcgt
     4261 cggctggagc ggcaacggat gcggatcggg cagcgtcacg gtcgcgcggc cggccgtcac
     4321 ctgcaccgcg aatttcgaca acgtgtcgcc ttcgacagga tcggccggcg gcagcggttg
     4381 cttcgttgcc accgccgcct acggcagcgc catggcgccg caagtggtcg cgctgcgcga
     4441 gttccgcgat cgctacctgc tggccaacgc acccggacgg gctttcgtac ggtggtatta
     4501 ccagtggtcg ccgggcgcgg cgcacgtgat ccgtggcgac gagaccctgc ggaagctcgc
     4561 acgagccgcg ctctggcccg cggtggcggc ggtgacgcac ccgaagctcg catgggcggc
     4621 gtcggccctg ctgctatggc tggcggtgga actggcggtg cacatgagcc gcgcaagaac
     4681 ggcgagatag actttcggac gttcgacggg gcggccatca tgctcgctgc atgagcgacg
     4741 tccccaccaa gtccgccgcc cgacccgagc ccgaccggct gctcgtcgag atcgccgact
     4801 acgcgatgaa ttacgacggt ggctcgcccc tcgcgcgcga gacggcctac cactgcctgc
     4861 tcgactcgct cgcctgcgcg ctgcaggcgc tgaagtatcc cgcctgcacg aaattgctgg
     4921 gtccggtcgt gcccggcgca acgatggccc gcggcg
//
LOCUS       DASPFR010000208         7254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1646773, whole genome shotgun sequence.
ACCESSION   DASPFR010000208 DASPFR010000000
VERSION     DASPFR010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7254
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646773"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1819
                     /locus_tag="VD737_05340"
     CDS             173..1819
                     /locus_tag="VD737_05340"
                     /inference="COORDINATES: protein motif:HMM:NF015194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HXW10018.1"
                     /translation="MLTDHAGLSRFERLLGLITRVRPGEGRSAFLFFLHGFLLLSSYQ
                     IVKALREALILTKFSAEVRAYAVAVMALALIFIVPLYGYVRRHVDGERLLRLVTLFFV
                     VTLPLFAGLVWAGAPVEFVFFVWVGIYGLMVISQMWAFAADSFNIKSGQRLFPVIMIG
                     ANLGALMGAKFAQFAAATLSVAGLMIFATGTLAATLLLAGPERDAVPEGSRSVSAPHG
                     MAVPRLLGGIGLVLRDRYLLQIALLAILLNWINSTGEFILADFFKADAERRVAADPSL
                     DMGSLITAYYGNFQFWVTVGSLAIQLLLVARIFRTVGIRGALLVHPGFVALGYGLIAL
                     APLLGGFIPIFSMIFFVKIVENSLDYSLMNTTRQALFLPVDRNSKYDGKTAIDTFFWR
                     FGDLIQAGAIYAGTHLLAWDATQFALLNFGLALAWIGLAVALGRGYGLKAQENVINVA
                     PEAGDPIPDLQYLPGERFLHPIAITAFRDADPGDVLQLRACCDDGQPLPRWLQFDPRR
                     RAFVGVGPVTMEEVRVLVIASDVDGMEARSDFRVRRVTLG"
     gene            1931..7234
                     /locus_tag="VD737_05345"
     CDS             1931..7234
                     /locus_tag="VD737_05345"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXW10019.1"
                     /translation="MATECSTPPLQSVPWLQGGGELGELIRAKDWSSTALGPPESWPG
                     SLRTAVSICLGSKFPILLWWGPDFVMLYNDGYREMLGAKHPRSLGQHGSECWAEIWHI
                     IGPMLDGVRSRGEATWSDDQMLPLYRIGFTEECYFTYTYSPIRNDAGRVEGVFCAVME
                     TTERVLNARRLATLRALAANAPEAHNATIACCDAVTTLVATPEDTPLAAMYLLSDDGR
                     TLDRVADAGLEDGDASLPVTFRLGDAEGELESALAAACTSEVAEACRIVAVADARLPA
                     LPSRPWPDPVQNVVCLRVKQPGQPRPSGVLLVAISPRRPLDPAYRSFLELAAGHVATA
                     IANSRSHEEERRRARMLAELDRAKTTFFTNVSHEFRTPLTLMLGPLEHVLALGKDELP
                     ERLRTELQTIYRNALRLLKLVNTLLDFSRIEAGRADASFEPTDLASFTAELAGVFRST
                     VEGAGLRYTVECEPLPEPVHVDRQMWEKVVFNLLSNALKFTLSGAIAVRLRAEGASVE
                     LAVADTGLGIAAEDQPRVFERFHRLTQRGARTQEGTGIGLALSQEFVRLHGGTLSVDS
                     EPGRGSVFTVRLPFGHGHLPPEHVRPHPRGAHAVRSSAFVNEAVGWAASAPSPRPAVE
                     PAADVPDRPRVLVADDNADMRGYLARICGSRWDVETVTDGAAALARIQRDPPDLLISD
                     VMMPGLDGLTLVRRLRANTATRELPVILLSARAGEEARLEGLQAGASDYLVKPFSARE
                     LLAYVDTRLEIAATRRNAEQGLRQSSALNEIQRDTLALALGGAPVEAVMQALADSGPK
                     LLGESARTAVYLRDGGTLRFGASANLPARYATEARLIPIDAVALPCGRAAHWGRAEIV
                     QDLAADPACAAYVPLAEALGVRSCWSIPIRAGEGRVIGTYAVYRPVPGGPSAAEIERV
                     TLLTQTAAVVLEHYASARVRADAETTLRESEHQLNAALELANASRRELESLLDAAPLG
                     VYVVDADAKIRHANPIAKDTFGLQHDVIGSDFVSEVERLWPTDHVREIVDLVRHTLAT
                     GQCHVAPERMQVRRDTGVVEWFEWRIDRIPLADGRPGVVCYYRDISMHVRARKALARA
                     ETLTSGQKRALELSLNGGAIDDVLRVLAQTGYEVSLDQAPTAIFRTTPDGHGHLVASA
                     GLAESYVKEFNESNLIEVSPSRRAILHGEMVVVEDVRSDPSCVPYRTIAQAYDVLSLW
                     SFPIRAPDGTILGALTVYRHEPGVPQTEDVQSMRLLADTAGMLLERDRVVAARAAAEE
                     ALRESERRIRELADAMPQLVWTSDATGKVDYYNGRAAQYDGIQPGDAPGASWQSIIHP
                     DDLEATVAAWERSTRLGVPYVFEHRLRMADGSYRWHLERAEPVRSANGLRWFGTATDV
                     HELRAAREALRDNEERLRDADRRKDEFIAMLAHELRNPLAPIRTGLQVIQRAPERVAE
                     VGRLGQIMERQVTHMVRLIDDLLDVSRITSGKLVLQRQPSLLQDLVAGAVEANRSGIA
                     TAGLDLAVQVPEEPLLIDVDPTRFLQVVSNLLNNAAKFTPAGGHIRLAAEVEGEGDAA
                     QLVLRVTDTGIGIRPDMLQRVFEMFTQAESELPRSNSGLGIGLALARQLIEMHGGTLQ
                     ACSDGPGRGSTFTVQLPLGRTPVTRRAARAPAPSANYTRRVLIVDDNEDAAEALAMLV
                     TDLGGAACTASDGFGGLERIGDFEPEIVLLDLGMPGIDGFETCRRMRDAGSRAYIVAL
                     TGWGQEKDRHQVLRSGFDAHLTKPADPAQLEELLATGRLKR"
BASE COUNT         1092 a         2511 c         2524 g         1127 t
ORIGIN      
        1 atggtaccgg catagtgacg accagcttca gcgcgccggt caagaacgag gcgggtggag
       61 tctacgaata cacgatcgag gtcaacgtgg gcgaagcagc atcgactgaa gcgtcgaccg
      121 cgttcctcga ccccatgatc atcgtcggac gcggctgagg gacgaccgca cggtgctgac
      181 cgaccacgct gggctcagcc gcttcgaacg actgctcgga ctgatcacgc gcgtccggcc
      241 tggcgagggc cggagcgcat tcctgttctt cctgcacggc ttcctgctgc tgtcgtcgta
      301 ccagatcgtc aaggcgctgc gcgaggcgct gatcctcacg aagttctcgg cggaggtgcg
      361 cgcctatgca gtggccgtga tggcgctcgc gctgattttc atcgtgccgc tgtacggcta
      421 cgtccgtcgc cacgtcgacg gcgagcgcct gctgcggctc gtgacgctgt tcttcgtcgt
      481 gacgctgccg ctcttcgcgg gcctggtctg ggccggggcc ccggtcgagt tcgtgttctt
      541 cgtgtgggtc ggcatctatg ggctgatggt catctcgcag atgtgggcct tcgcggccga
      601 ctcgttcaac atcaagagcg ggcagcgact gttcccggtc atcatgatcg gcgccaacct
      661 gggtgcgctc atgggcgcca agttcgcgca gttcgcggcg gccacgcttt ccgtcgcggg
      721 cctgatgatc ttcgcgaccg gcacgctcgc agcgacgctg ctgctggccg gacccgagcg
      781 cgacgcggtg ccggaaggat cacgctcggt ctccgcgccg cacggcatgg ccgtgccccg
      841 cctgctcggc ggcatcgggc tggtgctgcg tgaccggtac ctgttgcaga ttgcgctgct
      901 cgcgatcctg ctcaactgga tcaactcgac cggcgagttc atcctcgcgg atttcttcaa
      961 ggcagacgcg gagcgacgcg tcgcggcgga cccgtcgctc gacatgggtt cgctgatcac
     1021 cgcgtactac ggcaatttcc agttctgggt cacggtggga tcgctcgcga tccagctgct
     1081 gctggtggcg cggatcttcc gcaccgtggg catccgcggc gcgctgctgg tccatccggg
     1141 cttcgtcgcg ctgggctacg ggctgatcgc gctcgcgccg ctgctcggcg gattcatccc
     1201 gatcttctcg atgatcttct tcgtcaagat cgtcgagaac agcctcgact attcgttgat
     1261 gaacaccacg cgccaggcct tgttcctgcc cgtcgaccgc aactccaagt acgacggcaa
     1321 gaccgccatc gacaccttct tctggaggtt cggcgacctc atccaggcgg gagcgatcta
     1381 cgccggcacc cacctgctcg cgtgggacgc cacgcagttc gcgctgctga acttcggtct
     1441 cgcgctcgcc tggatcgggc tggcggtcgc gctcggccgc ggctacggcc tgaaggcgca
     1501 ggagaacgtc atcaacgtcg cgcccgaggc cggggacccg atcccggacc tgcaatacct
     1561 gcccggcgag cgcttcctgc acccgatcgc gatcacggcg ttccgggacg ccgacccggg
     1621 tgacgtattg cagctgcggg cgtgctgcga cgacgggcaa ccgctgcccc ggtggctcca
     1681 gttcgatccg cggcgtcggg cattcgtggg cgtcgggcct gtcacgatgg aagaggtccg
     1741 cgtgctggtc atcgcgagcg acgtggacgg aatggaggcg cgcagcgact tccgggtgcg
     1801 acgggttacg ctgggctgag gccagagggg tgcttgcccc aacgccgttg tcggttttgg
     1861 ctgacattgc gggccggccg ggcctcttat tctgtcggct tcggttcccc gccccgtgaa
     1921 ggacctcccg ttggccacgg aatgttccac cccacctttg cagtcggtgc cctggctgca
     1981 aggcgggggt gagctgggcg aactgatccg cgccaaggac tggtcgagca ccgccctggg
     2041 cccgccggaa tcgtggccgg ggagcctgcg cacggccgtc agcatctgcc tcggctcgaa
     2101 gttcccgatc ctgctctggt ggggtcctga cttcgtgatg ctctacaacg acggttaccg
     2161 ggagatgctg ggggcgaagc atccgcgctc gctgggccag cacggcagcg aatgctgggc
     2221 cgagatctgg cacatcatcg gcccgatgct cgacggcgtg cgaagccgcg gggaagccac
     2281 ctggtcggac gaccagatgc tgcccctgta caggatcggc ttcaccgagg agtgctactt
     2341 cacctacacg tacagcccga tccgcaacga cgccgggcgc gtcgaaggtg tgttctgcgc
     2401 ggtcatggaa acgaccgagc gcgtgctcaa tgcgcgccgg cttgcgacgc tgcgcgcgct
     2461 ggccgcgaac gcacccgagg cgcacaacgc gacgatcgcc tgctgcgacg ccgtcacgac
     2521 gctggtcgcg acgccggaag acacgccgct cgcggcgatg tacctgctga gcgacgacgg
     2581 gcgcacgttg gaccgcgtgg cggacgccgg cctggaggac ggcgacgcgt cgctgccggt
     2641 cactttcagg ctcggcgatg ccgagggcga actcgaaagc gcgctcgcgg ctgcctgcac
     2701 gagcgaagtc gcggaagcct gccgcatcgt ggccgtcgcc gacgcgcgcc tgccggcgtt
     2761 gccgagccgg ccatggccgg atcccgtgca gaacgtcgtc tgtctacgcg tcaagcagcc
     2821 cggacagccg cgcccgagcg gcgtcctgct ggtcgccatc agcccccggc ggccgctcga
     2881 tccggcgtac cgcagcttcc tggagctggc ggcgggccac gtcgcgacgg ccatcgccaa
     2941 ctcgcgcagc cacgaggaag agcgccgccg cgcccggatg ctcgcggaac tcgaccgcgc
     3001 caagaccacg ttcttcacca acgtgagcca cgagttccgc acgccactca cgctgatgct
     3061 ggggccgctc gagcacgtgc tcgcgctggg caaggacgag ctgccggagc gcctgcgcac
     3121 ggagctgcag acgatctatc gaaacgcgct gcgcctgctg aagctcgtga acacgctgct
     3181 cgacttctca cggatcgagg cgggtcgcgc cgacgcgagc ttcgagccga cggacctcgc
     3241 aagcttcacc gccgagctcg ccggcgtctt ccggtccacg gtcgagggcg ccggactgcg
     3301 ctacacggtc gagtgcgagc cgttgccgga gcccgtccac gtcgatcgcc agatgtggga
     3361 gaaggtcgtc ttcaacctgc tctcgaacgc cctcaagttc acgctgtcgg gtgccatcgc
     3421 ggttcgactg cgggccgaag gcgcgtccgt cgaactggcc gttgcggaca ccggactcgg
     3481 cattgccgcg gaagaccagc cacgcgtgtt cgagcgcttt caccgtctca cgcagcgcgg
     3541 cgcccgcacc caggaaggca ccggcatcgg cctggcgctg tcgcaggagt tcgtgcggct
     3601 gcacggcggc acgttgagcg tcgacagcga gccggggcga ggctcagtgt tcaccgtcag
     3661 gttgccattt ggacacgggc acctgccccc ggagcatgtc cggccgcacc cgcggggagc
     3721 gcacgccgtc cgcagctcgg ccttcgtgaa cgaggccgtc ggctgggcgg cgagtgcccc
     3781 gtcgccgcgt ccggcggtcg agccggctgc cgacgtcccg gatcgtcccc gggtgctcgt
     3841 ggccgacgac aacgcggaca tgcgcgggta tctcgccagg atctgcggca gccgctggga
     3901 cgtcgagacg gtcacggacg gcgccgctgc gctggcccgg atccagcgtg accccccgga
     3961 cctgctgatc tcggacgtga tgatgcccgg cctcgacggc ctgacgctcg tccgccggct
     4021 tcgtgcgaac acggcgaccc gggagctgcc agtcatcctg ctgtcggcgc gggcgggcga
     4081 ggaagctcga ctggaggggt tgcaggcagg cgcgagcgat tacctggtca agccgttctc
     4141 ggcgcgggag ctgctggctt acgtcgacac gcgcctggag atcgcggcga cccgccgcaa
     4201 cgccgagcag ggactgcgcc agagttcggc gctcaacgaa atccagcgcg ataccctggc
     4261 gctcgcgctc ggaggtgcac ccgtcgaggc ggtgatgcag gcgctggccg actccggtcc
     4321 gaagctgctc ggcgagtccg ctcgcacggc cgtgtacctc agggacggcg gaacgctgcg
     4381 gttcggcgct tccgccaacc tgccggcgcg ctatgccacg gaggcgcgcc tgatcccgat
     4441 cgacgcggtc gcgctgccct gcggccgcgc ggcgcactgg ggccgggcgg agatcgtcca
     4501 ggacctcgcg gccgacccgg cctgcgcggc gtatgtgccg ctcgccgaag cactcggcgt
     4561 gcggtcctgc tggtcgatcc cgatccgcgc gggggaggga cgggtgatcg gcacctacgc
     4621 cgtgtatcgg ccggtgccgg gcggcccgtc cgcggccgag atcgagcgtg tgacgctgct
     4681 gacgcagacg gcggccgtcg ttctcgagca ctacgcgagc gcacgcgtac gcgccgacgc
     4741 cgagacgacg ctgcgcgaga gcgagcacca gctgaacgcg gcgctcgagc ttgccaacgc
     4801 cagccgtcgc gagctcgaaa gcctgctcga cgctgctccg ctcggcgtgt acgtcgtcga
     4861 cgcggacgcg aagatccgcc acgccaaccc catcgccaag gatacgttcg gactgcagca
     4921 cgacgtgatc ggctcggatt tcgtcagcga agtcgaacgg ctctggccca ccgaccacgt
     4981 gcgtgaaatc gtcgacctgg tgcggcacac gctcgccacc ggccagtgcc acgtcgcgcc
     5041 ggagcgcatg caggtgcggc gcgacacagg cgtcgtcgaa tggttcgagt ggcgcatcga
     5101 ccgcatcccg cttgcagacg gccgtccggg cgtcgtctgc tactaccgcg acatctcgat
     5161 gcacgtacgc gcgcgcaagg cgctcgcccg cgcggagacg ctgacctcgg gccagaagcg
     5221 tgcgctcgag ctgtcactca acggcggtgc gatcgacgac gtgctgcgcg tgctggccca
     5281 gaccggctac gaagtctcgc tcgaccaggc gcccacggcg atcttccgga ccacgccgga
     5341 cggccacggc cacctcgtcg cctccgccgg cctcgccgag agctatgtga aggagttcaa
     5401 cgagtcgaac ctgatcgagg tgtcaccgag ccggcgtgcg atcctgcacg gggaaatggt
     5461 cgtcgtcgag gacgtgcggt cggaccccag ctgtgtgccg taccgcacga ttgcgcaggc
     5521 gtacgacgtg ctgtcgctgt ggtcgttccc gatccgggcc ccggacggca cgatcctcgg
     5581 ggcgctgacc gtttaccggc acgagcccgg cgtgccgcag accgaagacg tgcagtccat
     5641 gcgcctgctc gcggacaccg caggcatgct gctcgagcgc gaccgggtcg tcgccgcgcg
     5701 tgcggcggcg gaggaagcac tgcgcgagag cgagcggcgc atacgcgagc tcgccgacgc
     5761 gatgccgcag ctcgtgtgga cgtccgatgc cacgggcaag gtcgattact acaacgggcg
     5821 cgcagcgcaa tacgacggca tccagccggg cgacgcaccc ggcgccagct ggcaatcaat
     5881 catccacccg gacgacctcg aggccacggt cgccgcctgg gagcgatcga cgaggctggg
     5941 cgtgccgtac gtgttcgagc atcgattgcg catggcagac ggcagctacc gctggcatct
     6001 cgagcgcgcg gagcccgtcc gcagcgccaa cggcttgcgc tggttcggca cggcgacgga
     6061 cgtgcacgaa ctgcgtgccg cacgcgaggc gctgcgcgac aacgaggaac gtctgcgcga
     6121 cgccgaccgt cgcaaggacg agttcatcgc gatgctggcc cacgaactgc gcaacccgct
     6181 cgcgccgatc cgcacggggc tgcaggtgat ccagcgtgcg cccgagcgcg tggccgaagt
     6241 cggccggctc gggcagatca tggagaggca ggtcacgcac atggtgcggt tgatcgacga
     6301 cctgctcgat gtctcccgga tcacgtccgg caagctcgtg ctgcagcgcc agccgagcct
     6361 gctgcaggat ctcgtggcgg gcgcggtcga ggctaaccgt tccggcatcg cgaccgccgg
     6421 tctcgacctg gctgtgcagg tgccggagga gccgctgctg atcgatgtcg acccgacgcg
     6481 gtttctccag gtcgtctcca acctgctcaa caatgcggcc aagttcacgc ccgcgggcgg
     6541 gcacatccgg ctcgcggcag aggtcgaggg cgaaggtgac gccgcgcagc tcgtcctgcg
     6601 ggtgacggac accggcatcg gcatccgccc cgacatgctg cagcgcgtgt tcgagatgtt
     6661 cactcaggca gagtccgagc tgccgcggtc caacagcggc ctcggcatcg gcctcgcgct
     6721 cgcgcgccaa ctgatcgaga tgcacggtgg cacgctccag gcctgcagcg acggccccgg
     6781 gcgcggcagc accttcaccg tgcaactgcc gctcgggcga acgcccgtca cgcggcgcgc
     6841 tgcacgcgcg cccgcgccca gcgccaacta cacgcgccgg gtgctgatcg tcgacgacaa
     6901 cgaggatgcc gccgaggcgc tggcaatgct ggtgaccgac ctcggcgggg ccgcgtgcac
     6961 cgccagcgac ggcttcggcg gcctcgagcg gatcggggac ttcgagcccg aaatcgtact
     7021 gctggacctc gggatgccgg gcatcgacgg cttcgagacc tgccgtcgga tgcgcgatgc
     7081 cggctcccgc gcctacatcg tggcgttgac cggctggggg caggaaaagg accggcacca
     7141 ggtcctgcgc agcggcttcg acgcgcacct gaccaagccc gcggatccgg cacagctcga
     7201 ggaactgctg gcgaccggac gactcaagag gtgatcggtg gccgaggggt agtg
//
LOCUS       DASPFR010000209         3024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1402263, whole genome shotgun sequence.
ACCESSION   DASPFR010000209 DASPFR010000000
VERSION     DASPFR010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402263"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VD737_05350"
     CDS             complement(<1..411)
                     /locus_tag="VD737_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10020.1"
                     /translation="MQQKTVVVDKVASIAQACGLGRELRIATSDIPCEEGVVLVVEVL
                     NNKSTYNTLELTSGRMAKVSRGDVIVGALGHRQALFGYSGHVPDTVRAGDVVQVLNIG
                     GVLGVCDSINPEKGKPFDARVLGCVLQFPFLGERI"
     gene            complement(440..1660)
                     /gene="argG"
                     /locus_tag="VD737_05355"
     CDS             complement(440..1660)
                     /gene="argG"
                     /locus_tag="VD737_05355"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HXW10021.1"
                     /translation="MTTTAQGARQPILLAFSGGLDTSFCVPWLAETYSRPVVTLTVDT
                     GGIDAEAAAALEQRSRALGAVAHHLVDARAAYFDQVLKYLVMGNVRRGNLYPLCVGAE
                     RVLQAQTVAQFARELGTDVAAHGSTAAGNDQVRFEVALRTLAPELEILAPVRDRAFKR
                     PEQLEYLRSRNLPVPPFGAAYSVNRGLWGVTIGGKETLVSDACIPDAAWVLTRDAFER
                     PQAPERHTLGFEAGVPCSLDGRRLDPVALIEQVEAIGARFGVGRGIHLGDTIIGFKGR
                     VAFEAPAAEVLLTAHRELEKLVTSARQQRTKDLLAGPYGDLVHEGQHLDPVCRDIEAF
                     FVSTQRRVTGEVHLLFRTGVVFVEGTSSPYSLMKASKGAYGESAGEWTPEDAHGFSKM
                     LALPGIFYTRAGRN"
     gene            complement(1670..2125)
                     /locus_tag="VD737_05360"
     CDS             complement(1670..2125)
                     /locus_tag="VD737_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10022.1"
                     /translation="MASQIEAQTLRRDAIVRILRQGAVARQAELVDLLAREGFDATQS
                     SVSRDLRELGVVKGADRYLLPAVEDALTPSHFETVRAFVKGYRAAGAALTVLRTSAGT
                     AQSVAIALDKARWPEVVGTIAGDDTIFIATGSARAQRRLHERLRTTFGR"
     gene            2208..2867
                     /locus_tag="VD737_05365"
     CDS             2208..2867
                     /locus_tag="VD737_05365"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXW10023.1"
                     /translation="MTADASSTGEGELAARRLALFRSLHALARTGLHFCRDEYDRERY
                     EQLEAIAAELLATGAGLTPAELRRDWAGETGYVTPKIEVRGAVFRADDGKVLLVREAL
                     DGLWTLPGGWADVNDSPSGAVRREIEQEAGLHTRVVKLAALYDRNAHGHTPSIHHSWK
                     AFFVCEVEGGELRGSYETDAVAFFDPSALPPLSLGRCTPAQVLRMRQHWLQPGIPTDF
                     D"
BASE COUNT          474 a         1022 c         1071 g          457 t
ORIGIN      
        1 gatgcgctcg cccaggaacg ggaactgcag cacgcatccg agcacgcgcg cgtcgaaggg
       61 cttgcccttc tccgggttga tcgagtcaca gacgccgagc acgccgccga tgttcagcac
      121 ctgcacgacg tcaccggcac gcacggtgtc cggtacgtgg ccggaatagc cgaacaatgc
      181 ctggcggtga ccgagcgcgc cgacgatcac gtccccgcgc gacaccttgg ccatgcggcc
      241 gctcgtcagc tcgagcgtgt tgtaggtcga cttgttgttg agcacctcga cgaccagcac
      301 cacgccttcc tcgcacggga tgtccgaggt ggcgatccgc agctcgcgac cgaggccgca
      361 ggcctgggcg atcgaggcga ccttgtcgac gacgacagtc ttctgttgca tcgtggcgag
      421 tcccccaggg agcggcgcgt cagttgcgtc cggcgcgcgt gtagaaaatg cccggcagcg
      481 ccagcatctt cgagaagccg tgtgcgtcct cgggcgtcca ttccccggcc gactcgccgt
      541 aggcgccctt ggacgccttc atcagcgagt acggggacga agtgccttcg acgaacacga
      601 cgcccgtccg gaacagcagg tggacttcgc cggtcacgcg ccgctgcgtg gagacgaaga
      661 acgcctcgat gtcgcggcag accgggtcga ggtgctgtcc ctcgtggacc aggtcgccgt
      721 aaggtcctgc cagcaggtcc ttggtgcgct gctgccgcgc ggacgtcacg agcttctcga
      781 gctcccggtg ggccgtcagc agcacctcgg ccgctggcgc ctcgaacgcg acgcgcccct
      841 tgaacccgat gatcgtgtcg cctaggtgga tgccacgccc gacgccgaac cgcgcgccga
      901 tcgcctcgac ctgctcgatc agcgcgacgg gatcgaggcg gcgaccgtcg agtgagcagg
      961 gcacgcccgc ctcgaagccg agcgtgtggc gctccggtgc ctgcggccgt tcgaaggcgt
     1021 cccgggtgag cacccaggcg gcgtcgggta tgcaggcgtc ggagaccagc gtctccttgc
     1081 cgccgatcgt cacgccccaa agcccgcggt tgaccgagta cgccgcgccg aacggcggca
     1141 ccggcaggtt gcgcgagcgc aggtattcga gctgctcggg ccgcttgaag gcgcgatcgc
     1201 ggaccggcgc gaggatctcg agctcgggcg cgagcgtgcg cagggccact tcgaagcgga
     1261 cctggtcgtt gccggccgcc gtagagccgt gcgcggcgac gtcggtgccg agctcgcggg
     1321 cgaactgtgc gacggtctgc gcctgcagga cgcgctcggc gccgacgcac agtgggtaca
     1381 ggttcccgcg gcgcacgttg cccatcacga gatacttgag cacctggtcg aagtaggcgg
     1441 cgcgcgcgtc gacgagatgg tgcgccaccg cgccgagcgc ccgcgagcgc tgctcgagcg
     1501 ccgcggcggc ctcggcgtcg atgccgccgg tgtcgacggt gagcgtgacg accggacgcg
     1561 aatacgtttc cgcgagccac ggcacgcaga acgaggtgtc gagtccgccg gagaatgcga
     1621 gcaggatggg ctgccgggcg ccctgggcag tggtggtcat cgagaggtct cagcgtccga
     1681 aggtggtgcg gaggcgctcg tgcaggcgac gctgcgcgcg ggcgctcccc gtggcgatga
     1741 agatcgtgtc gtcgccggcg atcgtgccga cgacttcggg ccagcgtgcc ttgtcgagtg
     1801 cgatggcgac gctctgggcc gtgccggccg aagtgcgcag gaccgtgagc gcggcgcccg
     1861 cggcgcggta gcccttcacg aacgcgcgca cggtctcgaa gtggctcggc gtcaatgcgt
     1921 cctcgaccgc gggcagcagg tagcggtcgg cacccttgac cacgccgagc tcacgcaggt
     1981 cgcggctgac gctggactgc gtggcgtcga agccttcgcg cgcgagcagg tcgaccagct
     2041 cggcctgacg cgcgaccgcg ccctggcgca ggatccgcac gatggcgtcc cggcgcaggg
     2101 tctgggcttc gatctggctg gccaacggcg gcgtccccgg tcaggtgaat aaaaggcgag
     2161 caaatgtgca taaatatgca agcaatgtca atggccccgg tgcacggatg actgcggacg
     2221 cctccagtac cggtgaaggt gaactcgcgg cgcggcgtct cgcgttgttt cgcagcctgc
     2281 acgcgctcgc gcggaccggg ctgcatttct gtcgcgacga gtacgatcgc gaacgctacg
     2341 aacagctcga ggcgatcgcg gccgagctgc tcgcgactgg cgcaggcctg acgccggccg
     2401 agctgcgccg tgactgggcc ggcgagaccg ggtacgtcac gccgaagatc gaggtccgcg
     2461 gcgccgtgtt ccgcgccgac gacggcaagg tcctgctggt tcgagaggca ctggacgggc
     2521 tctggacgct gcccggaggc tgggcggacg tcaacgactc gccgtccggc gcagtacggc
     2581 gggagatcga gcaggaagcg ggcctgcaca cgcgcgtcgt caagctcgcg gcgctctacg
     2641 accgcaacgc gcacggccac actccgtcga tccatcacag ctggaaggcg ttcttcgtct
     2701 gcgaggtcga gggcggcgaa ctgcgcggca gctacgagac cgacgccgta gccttcttcg
     2761 atccgtccgc gctgccgccg ttgtcgctcg gccgctgcac gccggcgcag gtcctgcgca
     2821 tgcgccagca ctggttgcag ccgggaatac ccactgactt cgattgaagc gggcagcttc
     2881 ggaaagtgtc tagtgttcag tgtctagcgg ttagtgggag cccacgaagc cggcgagcga
     2941 cgtagccgct tcctgcccgt cactagccac tagccactaa ccactagcca ctagccacta
     3001 gccactctcc atcaccgttc gatc
//
LOCUS       DASPFR010000210         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1402317, whole genome shotgun sequence.
ACCESSION   DASPFR010000210 DASPFR010000000
VERSION     DASPFR010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402317"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1589
                     /locus_tag="VD737_05370"
     CDS             <1..1589
                     /locus_tag="VD737_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008305243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXW10024.1"
                     /translation="MDRVVCGDVGFGKTEVALRAAFVAVQGGKQVAVLVPTTLLAQQH
                     YETFVDRFADWPVKIELMSRFRAGAQFKTAVEGLADGKVDVVVGTHRLLQPGIKFKDL
                     GLVIIDEEHRFGVRDKERLKALRAEVDVLTLTATPIPRTLNMALGGLRDLSLITTPPQ
                     ARLSIKTFVSEWSDPTIREACLRELRRGGQVYFVHNSVETIDRMGQRLTELVPEARIV
                     IGHGQMRERDLEQVMLDFYHKRANMLLCTTIIESGIDVPTANTIVIDRADRFGLAQLH
                     QLRGRVGRSHHQAYAYLVTPTKNAMTADAQRRLEAIESLEDLGAGFVLATHDLEIRGA
                     GELLGEEQSGQIQEVGFALYMEMLERAVKAMQDGKDLQLDKPLHHGPEIELHVPALLP
                     ESYLPDVHARLVLYKRISSVQSVPELDDLQAETVDRVGPLPEPARNLFRIARLRVAAT
                     PLAIERMDLGSSSGSVSFGTGTPLDPGALILMIQKSARTMRFDGPQKLRFTGQWEDPE
                     QRFSAAQALLDQLDRCVTRH"
     gene            1716..>2615
                     /locus_tag="VD737_05375"
     CDS             1716..>2615
                     /locus_tag="VD737_05375"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HXW10025.1"
                     /translation="MLAFLLLWLAGCASEAQLDVAEGIGPAPKLPPPVPERIPTVEIA
                     KATGWAEGRRPEPAAGLRVTAFAQQLEHPRWLHVLPNGDVLVAETAAPERPEENHGLR
                     GWLMKYFMKKAGAVVPSANRITLLRDADGDGRAEVRSVFIEGLNSPFGMALVGDDFYV
                     ANTDAVVRFPYQPGQRQITAAATKVASLPAGPRNHHWTKSLIASPDGRKLYATVGSNS
                     NVAEHGMAEEQGRASIWEIDRASGAAREYATGLRNPNGLAWVAETGALWTAVNERDEL
                     GGDLVPDYMTAVRDGGFYGWPYSY"
BASE COUNT          420 a          928 c          905 g          362 t
ORIGIN      
        1 cgatggatcg cgtcgtgtgc ggcgacgtgg gcttcggcaa gaccgaagtc gcgctgcggg
       61 cggccttcgt cgccgtgcag ggcggcaagc aggtggccgt gctggtgccg accacgctgc
      121 tcgcgcagca gcactacgag acgttcgtcg accgtttcgc cgactggccc gtcaagatcg
      181 agctgatgtc gcgctttcgc gcgggcgcgc agttcaagac ggccgtcgaa ggtctcgccg
      241 acggcaaggt cgacgtggtc gtgggcacgc accggctgct gcagcccggc atcaagttca
      301 aggacctcgg gctcgtgatc atcgacgagg agcaccgctt cggcgtgcgc gacaaggagc
      361 ggctcaaggc gctgcgcgcc gaggtcgacg tgctgacgct caccgccacg ccgatcccgc
      421 gcacgctgaa catggcgctc ggcggcctgc gcgacctgtc cctgatcacg acgccgccgc
      481 aggcgcggct gtcgatcaag acgttcgtca gcgagtggag cgacccgacg atccgcgagg
      541 cctgcctgcg cgagctgcgc cgtggcgggc aggtttactt cgtgcacaac agcgtcgaga
      601 cgatcgaccg gatgggccag cgactcaccg agctcgtgcc cgaggcgcgc atcgtcatcg
      661 gccacggcca gatgcgcgag cgcgacctcg agcaggtgat gctcgatttc taccacaagc
      721 gcgccaacat gctgctctgc acgaccatca tcgagagcgg catcgacgtg ccgacggcca
      781 acacgatcgt gatcgaccgc gccgaccgct tcgggctcgc gcagctgcac cagctgcgcg
      841 gccgcgtcgg ccgttcgcac caccaggcct acgcctacct ggtcacgcct acgaagaacg
      901 ccatgaccgc cgacgcccag cgacggctcg aggcgatcga gtcgctcgag gacctcggcg
      961 caggcttcgt gctagcgacc cacgacctcg agatccgcgg cgccggcgag ctcttgggcg
     1021 aggagcaaag cggccagatc caggaggtgg gcttcgcgct ctacatggag atgctcgagc
     1081 gcgccgtgaa ggcgatgcag gacggcaagg acctccagct cgacaagccg ctgcaccacg
     1141 gccccgagat cgagctgcac gtgcccgcgc tgctgccgga gtcgtacctg cccgacgtgc
     1201 acgcgcgcct ggtgctgtac aagcgcatct cgtcggtgca gtcggtgccc gaactcgacg
     1261 acctgcaggc cgagaccgtc gaccgcgtcg ggccgctgcc cgaaccggcc cggaacctgt
     1321 tccgcatcgc aaggctgcgc gtcgcggcca cgccgctggc catcgagcgc atggacctcg
     1381 gcagctcgag cggctcagtg agctttggga ccggcacccc gctcgatccg ggggcgctga
     1441 tcctgatgat ccagaagagc gcacgcacga tgcggttcga cgggccgcag aagctccgct
     1501 tcaccggcca gtgggaggac cccgagcagc gattctcggc ggcgcaggcc ctgctcgacc
     1561 agctcgaccg ctgcgtgacg cggcactgac gccgtcagca aacggcgcgc ccgcacgccg
     1621 gtgtacgtcc gcacgaccgc tgcgtcgcgg ccggagcgtg ttgtaatcgg ctcccgccgc
     1681 tgcggagccg tgccttgcgc catcgtcacc gcccagtgct tgcattcctg ttgctgtggc
     1741 tcgcgggctg cgcctccgag gcgcagctgg acgtcgccga gggcatcggg ccggccccga
     1801 agctgccgcc cccggtcccc gagcgcattc ccacggtcga gatcgcgaaa gcgaccggat
     1861 gggccgaagg acgccgcccc gaaccggccg cggggctgcg cgtcacggcg ttcgcgcagc
     1921 agctcgagca tcctcgctgg ctgcacgtgc tgccgaacgg cgacgtgctg gtggcggaga
     1981 ccgcggcgcc ggagcggccg gaggagaatc acggcctgcg gggctggctc atgaagtact
     2041 tcatgaagaa ggcgggcgcg gtggtgccga gcgccaatcg catcaccttg ctgcgcgacg
     2101 ccgacggcga cggtcgcgcc gaggtccgct cggtgttcat cgaggggctc aattcgccgt
     2161 tcggcatggc cctggtcgga gacgatttct acgttgccaa caccgacgcc gtcgtgcgct
     2221 tcccgtacca gccgggccag cgccagatca ccgcggccgc aacaaaggtc gcgtccctgc
     2281 cagccggccc gcgcaatcac cactggacca aaagcctcat cgcgagcccc gacggccgca
     2341 agctctacgc gacggtcggg tccaacagca acgtggcgga gcacggcatg gccgaggagc
     2401 aagggcgcgc gtcgatctgg gagatcgacc gcgcgagcgg cgcggcgcgc gagtacgcga
     2461 cgggcctgcg caacccgaac ggcctcgcct gggtggccga gaccggcgcg ctctggaccg
     2521 cggtcaacga gcgcgacgaa ctcggcggcg acctcgtccc cgactacatg accgccgtgc
     2581 gcgacggcgg cttctatggg tggccgtaca gctat
//
LOCUS       DASPFR010000211         4214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_181950, whole genome shotgun sequence.
ACCESSION   DASPFR010000211 DASPFR010000000
VERSION     DASPFR010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181950"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VD737_05380"
     CDS             <1..312
                     /locus_tag="VD737_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXW10026.1"
                     /translation="VVFNNGQWGAEKKNHVDFYSRRFLGVDLDSPSWARVAQAFGCEG
                     VTIDAPGDVGPALRQAVEAQAQGTCTVLELMLTQELGDPFRRDALSRPVRHLDKYKAY
                     V"
     gene            complement(322..1083)
                     /locus_tag="VD737_05385"
     CDS             complement(322..1083)
                     /locus_tag="VD737_05385"
                     /inference="COORDINATES: protein motif:HMM:NF036520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXW10027.1"
                     /translation="MMAASSSKQARRQVNRGARVRSGTRAPRRSKATQLDLRRTPMQA
                     RGQATFERILDATAELLDKVGTEALTTNLISRTADVNVATLYQYFPNKQAVLLALFKR
                     QNETRVEIGKQMISGLAHSPDWRRMIANGVQAVAEARRNAGGATALRLAMRSSPDLLE
                     HDRQQSVEIAGTLSRELVEAGLKPDKALLVARCTIEALTAVLDLWSIESGGKDDRILG
                     EAKELVCDHLASYFDAARKGRREGTRRGKAARPRR"
     gene            1268..2248
                     /gene="glyQ"
                     /locus_tag="VD737_05390"
     CDS             1268..2248
                     /gene="glyQ"
                     /locus_tag="VD737_05390"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HXW10028.1"
                     /translation="MPKTALPVPRTFQDLILALQRYWAERGCVILQPYDMEVGAGTFH
                     TATFLRAVGPEPWSAAYVQPSRRPTDGRYGDNPFRLQHYYQYQVVIKPSPDDFIDLYL
                     GSLRAVGIDPLVHDVRFVEDNWESPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLDCR
                     PVMGEITYGLERLAMYLQGVESVFDLVWTDGPLGRVTYGDVYHQNEVEQSKFNFELAD
                     VDELFRQFDAHEKECLALLEAGLALPAYERMLKTSHTFNLLDARKAISVTERQRYILR
                     VRTLARGVAEAYYASREALGFPMARNRGQSPIPHVDPPLPERGLATRGLA"
     gene            2245..>4214
                     /gene="glyS"
                     /locus_tag="VD737_05395"
     CDS             2245..>4214
                     /gene="glyS"
                     /locus_tag="VD737_05395"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HXW10029.1"
                     /translation="MTSARDFLVEIGTEELPPKSLLGLSAAFAEGIERGLRDAGVSFR
                     AVERFATPRRLAVRVRKLAEQQPDRPLEKRGPPVKAAFDAAGNPTQAALAFARGCSVD
                     VGSLERLDTPKGQWLVHRGVEAGARTADLLPAVVTAALDALPIAKRMRWGAGDAQFVR
                     PVHWVVMLYGREVVPAEILGVRAANLTYGHRFMAPRAIRLPSPLAYERQLLKRGRVLA
                     DAVARRDAIRRGVTEAAERVGGTAVVSEALLDEVTGLVEWPVPLAGRFDARFLDLPPE
                     VPIATMQDHQRYFPVRDAEGRLMPWFVTVSNIESLDPAQVVAGNERVVRPRLSDAEFF
                     YATDRRQRLEMRSEGLKRVTFQTQLGSLHQKTERVRSLARSIAATIGADVSLADRAAQ
                     LAKCDLLTNMVAEFPELQGLMGRYYARHDGEPAEVAEALREQYLPRFAGDELPTTGAG
                     MALSIADKLDTIAGIYATGQKPSGTRDPFGLRRAALGLLRTAIERGLDLDLQRLLRQA
                     VVAARADMETVAAGLGKSLPPADLDTLSDDVYDYVIERLRAWYVEGGAGLEVTPEMFD
                     AVLATRPASPLDFDARLRALVQFLQLPEATSLAAANKRIANILRKTDEPVGDVVDPDR
                     LLDPAEQVLAEQVDAIARMVEPRFASREYTQGL"
BASE COUNT          607 a         1470 c         1463 g          674 t
ORIGIN      
        1 gtggtcttca acaacggcca gtggggcgcg gagaagaaga accacgtgga tttctattca
       61 cgacgattcc tcggcgtcga cctggacagt cccagctggg cgcgcgtcgc gcaggcgttc
      121 ggctgcgagg gcgtcacgat cgatgcgccc ggcgacgtcg gaccggcctt gcgccaggcc
      181 gtcgaggcgc aggcgcaggg cacctgcacc gtcctcgagc tgatgctgac gcaggaactc
      241 ggcgacccgt tccgccgcga cgcgttgtcg cgacccgtgc gtcacctcga caagtacaag
      301 gcgtacgtgt aacgcccgcg atcaacgccg cgggcgcgcc gccttgccgc gtcgggtccc
      361 ctctcggcgg cccttgcggg cggcgtcgaa gtacgacgcg aggtgatcgc acacgagttc
      421 cttggcttcg ccgaggatcc ggtcgtcctt gccgccggac tcgatggacc agaggtccag
      481 caccgccgtg agcgcctcga tcgtgcagcg agcgaccagc agcgccttgt cgggtttgag
      541 ccccgcctcc accagctcgc gtgacagcgt gccggcgatc tccaccgact gctggcggtc
      601 gtgctcgagc aggtcgggcg aggagcgcat cgcaaggcgc agcgccgtgg cgccgccggc
      661 gttgcgccgg gcttcggcga ccgcctgcac gccattcgcg atcatgcgtc gccagtcagg
      721 cgagtgcgcg aggccgctga tcatctgctt gccgatctcg acgcgcgtct cgttctggcg
      781 cttgaacagc gcgagcagca cggcctgctt gttgggaaag tactggtaga gcgtggcgac
      841 gttgacgtcg gccgtgcgcg agatcaggtt ggtcgtgagc gcctcggttc ccaccttgtc
      901 gagcagctcg gcggtcgcat cgaggatccg ctcgaaggtc gcctggcctc gcgcctgcat
      961 cggcgtgcgc cgcaggtcga gctgggtggc cttcgaacgg cgcggtgccc gtgtcccgga
     1021 acgcacccgc gcgccccggt tgacctgcct gcgcgcctgc ttgctcgaac tcgctgccat
     1081 catgtcgctc cgtaatattg gccgccgcat ggtcgcgacg acccgttagt atcgcctcat
     1141 gtatgggtcc gcgtcaggtg cagtagtcct acacatctga atagtgcttg ggttcggccc
     1201 gtggccacgg gccgtgccgc ttcgctgcgt tgctatactt gctgcttttg cgccctcgcg
     1261 agcacccatg ccgaagacgg cccttcccgt cccgcgaacg ttccaggacc tgatcctcgc
     1321 gctgcagcgc tactgggccg agcgtggctg cgtgatcctg cagccttacg acatggaagt
     1381 cggtgccggc acgttccaca ccgcgacgtt cctgcgcgcc gtcggccccg agccgtggag
     1441 cgccgcctat gtgcagccgt cgcggcgccc gaccgacggc cgctacggcg acaacccttt
     1501 ccggctgcag cattattacc agtaccaggt cgtcatcaag ccgtcgcctg acgacttcat
     1561 cgacctgtac ctcggatcct tgcgcgcggt cggcatcgac ccgctcgtgc acgacgtccg
     1621 gttcgtcgaa gacaactggg agtcgccgac gctcggcgcg tgggggctcg gctgggaggt
     1681 ctggctcaac ggcatggagg tcacccagtt cacgtacttc cagcaggtcg gcggactgga
     1741 ctgccggccc gtgatgggcg agatcaccta cggcctggag cgcctcgcga tgtacctgca
     1801 gggcgtcgag agcgtgttcg acctcgtctg gacggacggt ccgctggggc gggtgaccta
     1861 tggcgacgtc tatcaccaga acgaggtcga gcagtcgaaa ttcaacttcg agctcgccga
     1921 cgtcgacgaa ctcttccggc agttcgacgc gcacgagaaa gagtgcctgg cgttgctcga
     1981 ggccgggctc gcgctgccgg cctacgagcg catgctgaag acctcgcaca cgttcaacct
     2041 gctcgacgcg cgcaaggcga tctcggtcac cgagcgccag cgctacatcc tgcgcgtgcg
     2101 gacgctcgcg cgcggggtcg ccgaggcgta ttacgcgagc cgcgaggcgc tggggtttcc
     2161 gatggcgcgg aatcggggac agtccccgat tccgcacgtc gatccgccac tgcctgagcg
     2221 aggtctcgcg acccggggcc tcgcatgacc tccgcgcggg acttcctggt cgagatcggc
     2281 accgaggagc tgcctcccaa gtcactgctc ggcctgagcg cggcgttcgc cgagggcatc
     2341 gagcgcgggc tgcgcgatgc cggggtgtcg ttccgcgcgg tggaacgttt cgcgacgcca
     2401 cgccggctgg ccgtgcgtgt ccgcaagctc gccgagcagc agccggaccg tccgctcgag
     2461 aagcgcggcc ctccggtcaa ggcggccttc gacgccgccg gcaacccgac ccaggcggcc
     2521 ctcgcgtttg cgcggggctg cagcgtcgac gtgggttcgc tcgagcgcct cgacacgccc
     2581 aagggccagt ggctggtgca tcgcggtgtc gaggccggtg cacgcaccgc cgacctgctg
     2641 ccggccgtgg tgaccgccgc gctcgacgca ttgccgatcg cgaagcgcat gcgctggggc
     2701 gcgggcgacg cgcagttcgt tcgacccgtg cactgggtcg tgatgctgta cggccgcgag
     2761 gtcgtgcccg ccgagatcct cggcgtgcgc gcggccaacc tgacgtacgg gcaccgcttc
     2821 atggcgccca gggccattcg cctgccgagc ccgctggctt atgagcgcca gctgctcaag
     2881 cgcggccgcg tgctcgcgga cgcagtcgcg cggcgcgacg cgatccgtcg cggggtcacc
     2941 gaggccgccg agcgcgtcgg cggaacggcg gtcgtgtccg aagcgctgct cgacgaggtg
     3001 acgggcctgg tcgagtggcc ggtgccgctg gccggccgct tcgatgcgag gttcctcgac
     3061 ctgccgcccg aggtgccgat cgcgaccatg caggaccacc agcgctattt cccggtgcgc
     3121 gacgccgaag gacggctgat gccctggttc gtcaccgtgt cgaacatcga aagcctcgac
     3181 ccggcgcagg tcgtcgccgg caacgagcgc gtcgtgcggc cgcgcctctc cgatgccgag
     3241 ttcttttacg cgaccgatcg ccggcagcga ctcgagatgc gcagcgaagg gctcaagcgg
     3301 gtcactttcc agacgcagct gggatcgctg caccagaaga ccgagcgcgt gcgctcgctg
     3361 gcgcgctcga tcgctgcgac gatcggcgcc gacgtgagcc tcgcggaccg ggccgcacag
     3421 ctcgcgaaat gcgacctgct cacgaacatg gtcgccgagt tccctgagct gcaaggtctg
     3481 atggggcgct actacgcccg gcacgacggc gaaccggccg aggtcgccga ggcactgcgc
     3541 gagcagtacc tgccccgctt cgcgggcgac gagctgccga ccaccggcgc gggcatggcg
     3601 ttgtcgatcg ccgacaagct cgacacgatc gccggcatct acgcgacggg ccagaagccc
     3661 tcgggcacgc gcgacccgtt cggcctgcga cgcgcagcgc tcgggctgct gcgcaccgcg
     3721 atcgagcgcg ggctcgacct cgacctgcag cgattgctgc ggcaggccgt cgtggcggcg
     3781 cgcgctgaca tggaaacggt cgccgccgga ctcgggaagt cgctgccgcc tgcggacctc
     3841 gatacgctgt ccgacgacgt ctacgactac gtgatcgagc gcctccgcgc ctggtacgtc
     3901 gagggcggcg caggactcga ggtcacgccc gagatgttcg acgcggtgct ggccacccgc
     3961 ccggcgtctc cgctcgactt cgacgcacgc ctgcgggcgc tggtccagtt cctccagctg
     4021 ccggaagcca cgagcctcgc ggcggcgaac aagcgcatcg cgaacatcct gcgcaagacc
     4081 gatgagccgg tgggcgacgt ggtcgatccg gatcgactgc tggaccctgc cgagcaggtc
     4141 ctggccgagc aggtggacgc gatcgcgcga atggtggagc cccgcttcgc gtcgcgcgag
     4201 tacacgcagg ggct
//
LOCUS       DASPFR010000212         5492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_304851, whole genome shotgun sequence.
ACCESSION   DASPFR010000212 DASPFR010000000
VERSION     DASPFR010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304851"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VD737_05400"
     CDS             complement(<1..253)
                     /locus_tag="VD737_05400"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HXW10030.1"
                     /translation="MMPDLLVHGLYVAYVLAFLLGLATIFTWVERKQSAVMSDRIGAN
                     RAYLRIPFTQVKLVWWGLFHGMADGLKMIFKENFTPATYD"
     gene            complement(250..1665)
                     /locus_tag="VD737_05405"
     CDS             complement(250..1665)
                     /locus_tag="VD737_05405"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXW10031.1"
                     /translation="MSAPAIKVTIDQREVEARPGETVLQVARRHGIDIPHYCYHDKLS
                     ISGNCRICLVKVNGLPKLQPACNLGCAPNMVVETAVEQVSQAREQVLQFLLLNHPVDC
                     GICDKAGECRLQDYQHLYGPKQSIARDPKHHKRKLYDLGPRITLDNERCILCSRCVRF
                     TREISGSNALGIVERGNHSYVEAEVPGRFDDPYSDNVIEICPVGALLSKDFLYKCRVW
                     FLQPVRSVCTGCSRGCSIKVWRRSAVRQQHMLGADSAHVAYRTTVFENPEINGPWLCN
                     KGYDQHKWMARDRLVQPLVRGQAGSAGAALAEAKRLLESARNPAVLVSSHASNEELDV
                     LQSLLGGVTVYVHRDWLPVEGEVVEDHLLIRADKNPNRAGVVARFGEREFDATAGHDV
                     VLAWGEFAAAPPGAAHWIHLSLFGTPADVPATVVIPVSNTWERRGTFTNFEGKLNAFE
                     QVFERPAGVQHAADVLRSLAS"
     gene            complement(1662..2942)
                     /gene="nuoF"
                     /locus_tag="VD737_05410"
     CDS             complement(1662..2942)
                     /gene="nuoF"
                     /locus_tag="VD737_05410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HXW10032.1"
                     /translation="MNGTVFQTFPITATSHTLGEYRTRGGYGGLEQALREWTPAQVGE
                     RAKASGLQGRGGAGFMTGLKWEFVAKDAPVVYLCCNIDEGEPGTFKDRWILEHSPHQL
                     IEGMLIASYALRVRHAFAYIRGEFDLPLRRLEAALQEAYAAGLVGENILGSGFSCDIV
                     VHRGAGAYVCGEESSLINSLEGKKGFPRNKPPFPAVRGLYQKPTVVNNVETLATVPWI
                     MVHGPEAYAAIGVRKSPGTRLFGVSGHVERPGLFEKPTGYPLAQLILEDAGGVLGGRP
                     LKAVIPGGSSTPILTADEIETVTLDPESMTRAGSMVGSGAVIVIAEGTCMVRLLQVLS
                     RFYAHESCGQCSPCREGTDWMNRILERVIAGQGTAADLATLRRIPAGIQGNTVCALGD
                     AASMPVASFMKKFGEEFEHYVTHGRSMHDGRLTP"
     gene            complement(2939..3418)
                     /locus_tag="VD737_05415"
     CDS             complement(2939..3418)
                     /locus_tag="VD737_05415"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HXW10033.1"
                     /translation="MNSPLPPALLEQFDAIRRRYPPEMKTSLVLPLLHCVQDARGHVT
                     EADAAAIAEYIGMPAIQVVEALTWYSMFDREPQGRYVIKVCRNIACSLRGSERVVAHL
                     QKKLGIGVGETTADGRFTLRTVECLASCGTAPAMQVNRTYHEQLDEARLDRILESLP"
     gene            complement(3415..4647)
                     /gene="nuoD"
                     /locus_tag="VD737_05420"
     CDS             complement(3415..4647)
                     /gene="nuoD"
                     /locus_tag="VD737_05420"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HXW10034.1"
                     /translation="MQLPPETRQYGPPVRSHLEHTADPNHVVVNIGPSHPATHGTIQI
                     IAEIDGEKVVRADVHCGYLHRGFEKEAEHHTYHNVIPYTDRLNYCSAMNNNFAYAEAV
                     EKLLGVELTPRCIWLRTLLAEYNRIADHITGIAASAMEMGGMTAFLYLMTVRDYIFEH
                     MNHLSGARLTYSYVRIGGLARDLPDGWLDRLEEILQFTEAYLERVHGMLDRNRIFIGR
                     TRDVAVLTKEQALHWGYTGPILRSTGMPMDLRKDAPYLAYAELDFEVPVGIKGDNYDR
                     YYVRMREIDESISMVRQCVKRLPGGPINVDDPRVVLPEKSLVYSQIESLIHHFKLVTE
                     GVKVPPGEVYSAHEAPNGELGFYLVSDGTGRPWKLHVRGPSFVHMGGMHTLLEGYQVS
                     DIVPTFGSINMVGGECDR"
     gene            complement(4655..5131)
                     /locus_tag="VD737_05425"
     CDS             complement(4655..5131)
                     /locus_tag="VD737_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HXW10035.1"
                     /translation="MTPGFYDSITAGLQGLYDRAWDSHGMLVLDLPAASLLPVVERLK
                     GEFGFALFLDVTAIDWLGRREPRFDVVYHFLCPAEMRRIRLKVALEEPALEVPTLTGL
                     YGSAHFMEREAHEMYGVRFAGNADLRPILLYEGFEGHPLRKDYAMEHEQPIVPYRK"
     gene            complement(5136..>5492)
                     /gene="nuoB"
                     /locus_tag="VD737_05430"
     CDS             complement(5136..>5492)
                     /gene="nuoB"
                     /locus_tag="VD737_05430"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HXW10036.1"
                     /translation="RFGSGPFRATPRQADLMIVAGTVNHKMGPRVKRLYDQMPEPKYV
                     ISMGSCSNCGGLFQQAYSVLKGIDQVIPVDVYVPGCPPRPEIVIDAIMRLQDRIASDR
                     HPILPSEQPPQSRTER"
BASE COUNT          846 a         1846 c         1894 g          906 t
ORIGIN      
        1 ggtcgtacgt ggccggggtg aagttttcct tgaagatcat cttcagcccg tcggccatgc
       61 cgtggaacag cccccaccag acgagcttga cctgcgtgaa cgggatccgc aggtaggccc
      121 ggttcgcgcc gatccggtcg gacatcacgg cggactgctt gcgctcgacc caggtgaaga
      181 tcgtcgcgag cccgagcagg aacgcgagca cgtacgcgac gtagagtccg tggacgagca
      241 ggtccggcat catgacgcga ggctccgcag gacgtccgcc gcatgctgca cgcccgccgg
      301 acgctcgaag acctgctcga acgcgttgag cttgccctcg aagttcgtga aggtgccgcg
      361 tcgctcccag gtattcgaca ccggtatcac gaccgtggcg ggcacgtccg ccggcgtgcc
      421 gaacaggctc aggtggatcc agtgcgcggc accgggcggc gcggccgcaa actcgcccca
      481 ggcgagcacg acgtcgtgac cggccgtggc gtcgaactcg cgctcgccga agcgcgcgac
      541 gacgccggcg cggttcgggt tcttgtcggc gcggatcaat agatggtcct cgacgacctc
      601 gccttcgacc ggcagccagt cgcggtgcac gtacaccgtg acgccgccga gcagcgactg
      661 cagcacgtcg agctcctcgt tcgacgcgtg cgaggacacg agcaccgcgg ggttgcgcgc
      721 cgactcgagc agccgcttcg cctcggcgag ggctgcgccg gcactgccag cctgcccgcg
      781 gacgagcggc tgtaccaggc gatcgcgcgc catccacttg tgctggtcgt agcccttgtt
      841 gcagagccac ggcccgttga tctccggatt ctcgaacacg gtcgtccgat aggccacgtg
      901 ggcgctgtcg gcgcccagca tgtgctgctg gcgcacggcg gagcggcgcc agaccttgat
      961 gctgcagccg cgcgagcagc cggtgcacac cgaccgcacg ggctgcagga accagacgcg
     1021 gcacttgtag aggaagtcct tcgacagcag cgcccccacc gggcagatct cgatcacgtt
     1081 gtccgaatac gggtcgtcga agcggcccgg gacctcggcc tcgacgtacg agtgattgcc
     1141 gcgctcgacg atgccgagag cgttcgagcc cgagatctcg cgcgtgaagc gcacgcagcg
     1201 gctgcagagg atgcagcgct cgttgtcgag cgtgatgcgc ggccccaggt cgtacagctt
     1261 gcgcttgtga tgcttgggat cgcgagcgat cgactgcttc ggtccgtaca ggtgctggta
     1321 gtcctgcagc cggcactcgc ctgccttgtc gcagatgccg cagtcgaccg ggtgattgag
     1381 cagcaggaac tgcagcacct gttcgcgcgc ttggctgacc tgctcgacgg cggtctcgac
     1441 gaccatgttc ggcgcgcagc ccaggttgca ggccggctgc agcttgggca acccgttgac
     1501 cttgaccagg cagatccggc aattgccgga gatggacagc ttgtcgtggt aacagtagtg
     1561 cgggatgtcg atgccgtggc ggcgcgcgac ctgcagcacc gtctcgcccg gccgggcctc
     1621 gacctcgcgc tggtcgatcg tgaccttgat tgccggtgcg ctcacggcgt gagcctcccg
     1681 tcgtgcatcg agcggccgtg cgttacgtag tgctcgaact cctcgccgaa cttcttcatg
     1741 aaactggcca ccggcatcga ggcggcgtcg ccgagcgcgc acacggtgtt gccctggatg
     1801 ccggccggga tccgccgcag cgtcgccagg tcggccgccg tcccctggcc cgcgatcacg
     1861 cgctcgagga tgcggttcat ccagtcggtg ccctcgcggc acggcgagca ctggccgcac
     1921 gactcgtgcg cgtagaagcg cgacagcacc tgcagcagcc gcaccatgca ggtgccctcg
     1981 gcgatcacga tcaccgcgcc ggagcccacc atgctgcccg ccctggtcat gctctccggg
     2041 tcgagcgtca ccgtctcgat ctcgtcggcg gtcaggatcg gcgtggagct gccacccggg
     2101 atcacggcct tgagcgggcg gccaccgagc acgccaccgg cgtcttcgag aatcagttgc
     2161 gcgagcggat agccggtcgg cttctcgaac aggcccggtc gctcgacgtg cccggacacg
     2221 ccgaacagcc gcgtgccggg cgatttcctg acgccgatcg ccgcgtacgc ttcaggcccg
     2281 tgcaccatga tccacggcac cgtcgcgagc gtctcgacgt tgttgacgac ggtcggcttc
     2341 tggtagagcc cgcgcaccgc cgggaacggc ggcttgttgc gcgggaagcc cttcttgccc
     2401 tcgagcgagt tgatcaggct cgactcctcg ccgcagacgt acgcgcccgc gccgcggtgc
     2461 acgacgatgt cgcagctgaa gccgctgccg aggatgttct cgccgaccag gcccgccgcg
     2521 taggcttcct gcagcgcggc ttcgagccgg cgcagcggca ggtcgaactc gccgcggatg
     2581 taggcgaagg cgtggcgcac gcgcagcgcg taggaggcga tcagcatgcc ttcgatcagc
     2641 tggtgcggcg agtgctcgag gatccagcga tccttgaacg tgccgggttc gccttcgtcg
     2701 atgttgcagc agaggtagac gaccggggcg tccttggcga cgaactccca cttgaggccc
     2761 gtcatgaatc cggcgccgcc gcggccctgc agccccgacg ccttggcgcg ctcgccgacc
     2821 tgcgccggcg tccactcgcg cagcgcctgc tcgagcccgc catagccgcc acgggtgcgg
     2881 tactcgccga gcgtgtgcga ggtcgcggtg atcggaaacg tctggaacac ggtgccgttc
     2941 acggcagcga ctccaggatc cggtcgagcc tggcctcgtc gagttgctcg tgataggtgc
     3001 ggttcacctg catcgcgggc gccgtgccgc aggaggcgag gcactcgacc gtcctgagcg
     3061 tgaagcgacc gtcggccgtc gtctcgccga cgccgatgcc gagcttcttc tgcaggtgcg
     3121 ccacgacgcg ttcggatccg cgcagcgagc aggcgatgtt gcggcacacc ttgatgacgt
     3181 agcgcccctg cggctcgcgg tcgaacatcg agtaccaggt gagcgcctcg acgacctgga
     3241 tcgccggcat gccgatgtac tcggcaatcg cggccgcgtc cgcctcggtg acgtgccccc
     3301 gtgcgtcctg cacgcagtgc agcagcggca gcaccagcga agtcttcatc tcgggcgggt
     3361 agcggcgccg gatggcatcg aactgctcga gcagcgccgg cggcagcggc gagttcatcg
     3421 gtcgcactcc ccgccgacca tgttgatcga gccgaacgtc gggacgatgt cggagacctg
     3481 gtagccctcg agcagcgtgt gcatgccgcc catgtgcacg aagctcggtc cgcgtacgtg
     3541 cagcttccag ggccggcccg tgccgtcgct gaccaggtag aagccgagtt cgccgttcgg
     3601 cgcctcgtgc gcgctgtaga cctcgcccgg cggcaccttg acgccctcgg tgacgagctt
     3661 gaagtggtgg atcagcgact cgatctgcga gtagaccagc gacttctcgg gcagcacgac
     3721 gcgcgggtcg tcgacgttga tcgggccgcc cggcaggcgc ttcacgcact ggcgcaccat
     3781 cgagatcgac tcgtcgatct cgcgcatgcg cacgtagtag cggtcgtagt tgtcgccctt
     3841 gatgccgacc ggcacctcga agtcgagttc ggcatacgcc aggtacgggg cgtccttgcg
     3901 caggtccatt ggcatcccgg tcgagcgcag gatcgggccc gtgtagcccc agtgcagcgc
     3961 ctgctccttc gtcagcaccg cgacgtcgcg cgtgcgcccg atgaaaatcc ggttgcggtc
     4021 gagcatcccg tggacgcgct cgaggtacgc ttcagtgaac tgcaggatct cctcgagccg
     4081 atcgagccag ccgtccggca ggtcgcgagc gaggccgccg atgcgcacgt acgaatacgt
     4141 gagccgcgcg cccgacaggt ggttcatgtg ctcgaagatg tagtcgcgca ccgtcatcag
     4201 gtacaggaag gccgtcatgc cgcccatttc catcgcagag gcggcgatgc cggtgatgtg
     4261 gtcggcgatc cggttgtatt cggcgagcag cgtgcgcagc cagatgcagc gcggcgtgag
     4321 ttcgaccccg agcagcttct cgaccgcctc ggcataggcg aagttgttgt tcatcgccga
     4381 gcagtagttg aggcggtcgg tgtacgggat gacgttgtgg taggtgtggt gctcggcttc
     4441 cttctcgaag ccgcggtgca ggtagccgca gtgcacgtcg gcgcgcacga ccttctcgcc
     4501 gtcgatctcg gcgatgatct ggatcgtgcc gtgcgtcgcc gggtgcgaag gcccgatatt
     4561 gacgacgacg tgattcgggt ccgcggtgtg ctcgaggtgg ctgcgcaccg gcgggccgta
     4621 ctgcctggtc tcgggaggaa gctgcatcac ggcgtcactt ccggtacggc acgatgggct
     4681 gctcgtgctc catcgcgtag tccttgcgca gcgggtgccc ctcgaagccc tcgtacagca
     4741 ggatcgggcg caggtcggcg ttgccggcga accgcacgcc gtacatctcg tgcgcctccc
     4801 gctccatgaa gtgcgccgac ccgtagagcc cggtcagcgt aggcacttcg agcgcgggct
     4861 cctcgagcgc caccttgagc cggatccgcc gcatctcggc ggggcagagg aagtgataga
     4921 ccacgtcgaa gcgcggttcg cggcggccga gccagtcgat cgcggtcacg tccaggaaca
     4981 gcgcgaagcc gaactcgccc ttgaggcgct cgaccacggg cagcaggctc gcggccggca
     5041 ggtccagcac gagcatcccg tggctgtccc acgcgcggtc gtacagcccc tgcaagccgg
     5101 ccgtgatgct gtcgtagaaa ccgggagtca agccgtcacc gttcagtccg cgactgcgga
     5161 ggctgctccg acggcaggat cgggtgacgg tcggacgcga tccggtcctg caggcgcatg
     5221 atcgcgtcga tcacgatttc gggccgcggc gggcagccgg gcacgtagac gtccacgggg
     5281 atgacctgat cgatcccctt gaggaccgag taggcctgct ggaagaggcc tccgcagttc
     5341 gagcaggagc ccatcgagat cacgtacttg ggctcgggca tctggtcgta gagacgcttc
     5401 acgcgcgggc ccatcttgtg gttcaccgtg cccgcgacga tcatgaggtc cgcctggcgc
     5461 ggcgtcgcgc ggaaggggcc cgagccgaag cg
//
LOCUS       DASPFR010000213         3629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_182119, whole genome shotgun sequence.
ACCESSION   DASPFR010000213 DASPFR010000000
VERSION     DASPFR010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182119"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1302)
                     /locus_tag="VD737_05435"
     CDS             complement(127..1302)
                     /locus_tag="VD737_05435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXW10037.1"
                     /translation="MAYENEARPVTDRGPDGPGRGVRHVEVTVDDAGTRLDNFLVRHL
                     KDVPRTHVFRLLRKGEVRVNKKRARPEQRVMLGDIVRIPPVRPAAEVPPPAAPRTASA
                     SLQQLVLDAIVYEDANLVVFNKPPGVAVHGGSATDFGLIEAFRAARPDVPDAELVHRL
                     DRETSGCLLVAKRRAALRDLHRQLREGTTEKRYLALLCGKWNLGTKRIELALDTDERR
                     SGERHVAVRSHGKLSVSTFKPVQFFGNLATLVEVEIDTGKTHQIRVHAAHAGHPVAGD
                     DKYGNRAFNERFRDYGLRRMFLHSSSIGFTRPGTLEPMLASAPLPAELGAVLDTLARG
                     KARAPSIPLPQARERSTEGRVRVTAPLPQSRERSTEGRVRAKPGGRRAARTRRSSKR"
     gene            1711..>3629
                     /locus_tag="VD737_05440"
     CDS             1711..>3629
                     /locus_tag="VD737_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HXW10038.1"
                     /translation="MLVNATQEEELRVALVDGQRIYDLDIEIPSRETKKANVYKGRIT
                     RVEPSLEAAFVDYGAERHGFLPLKEVSKLYFKEKQPGQDGGRGIRDLIEEGQEIIVQV
                     EKEERGNKGAALTTFISLAGRFLVLMPNNPRAGGVSRRIEGDDREQLREAMDALQIPD
                     GMGAIVRTAGVGRSPEELQWDLNNLVDVWNAIQAASDSRPSPFLIYRESTAILRALRD
                     YLADDIGEILVDKHEVFEEAREYMQRFMPNNLRRLKLYDDHVPLFTRYQIESQIESAY
                     SHKVTLPAGGSIVIDHTEALVSIDINSARSTRGTDIETTALNTNLEAADEIARQLRLR
                     DVGGLIVIDFIDMESQKNQRAVEDRLRDAVKQDRARIQLGRISRFGLLELSRQRLRPS
                     ISESTNIVCPRCNGMGVIRSVESLALAVLRLIGEEARKDRTAKVIAQLPVDVATYLMN
                     EKRDWLHDIENRSRVDIVLVPNKYLETPAYELRRVRDDEISLPENSMISHQMAIEPQI
                     DLDAIAQAEEEKAPPPTPAAVTSIVPSTPAPPAAPEPVEPPTSAPAPIAAAAAVAAAP
                     LYGPRDNVLVRLWRWIAGDKRAPEPEPAPIRTARPEGDRDRHRRDRDERGRGRDRDAR
                     RDGRRDERDGRRDARR"
BASE COUNT          616 a         1281 c         1206 g          526 t
ORIGIN      
        1 tccctcggca tcgcgttgtt cgagcggatc gactcgtccg acccgaccgg cctcacgggg
       61 ttgccgctcg cgtgggtctg cggcgcgctg cgacgcgccg ggttcgacgt gccgtagcgc
      121 cgcaattcag cgtttcgagg accttcgagt ccgggcggcg cgccggcccc cgggctttgc
      181 cctcacccgg ccttcggtcg acctctcccg cgattgcggg agaggtgccg tcaccctcac
      241 ccggccttcg gtcgacctct cccgcgcttg cggtagaggt attgaaggtg cgcgtgcctt
      301 gccgcgggcg agcgtgtcga gcactgcgcc gagttcggcc gggagcggcg cgctcgccag
      361 catcggttcg agcgtgcccg gacgcgtgaa gccgatcgag gacgaatgca ggaacatgcg
      421 acgcagcccg tagtcgcgga accgctcgtt gaacgcacgg ttgccgtatt tgtcgtcgcc
      481 ggcgaccgga tgtccggcat gggcggcgtg cacgcggatc tggtgcgtct tgccggtgtc
      541 gatctccacc tccaccagcg tcgccaggtt gccgaagaac tgcacgggct tgaaagtgct
      601 cacggacagc ttgccgtggg accgcaccgc cacgtgtcgc tcgccactgc ggcgctcgtc
      661 ggtgtccagc gcgagttcga tccgcttggt gccgaggttc cacttgccgc agagcaacgc
      721 gaggtagcgc ttctcggtcg tgccctcgcg caactgccgg tgcaggtcgc gcagcgccgc
      781 ccgtcgcttc gcgaccagca ggcagccgct cgtctcgcga tcgagccggt gcacgagctc
      841 cgcatcgggc acgtcgggac gggcggcgcg gaacgcctcg atcaggccaa agtccgtggc
      901 gctgccgccg tgcaccgcga cgcccggggg cttgttgaac accacgaggt tggcgtcctc
      961 gtacacgatc gcgtcgagca cgagctgctg cagcgaggcg ctggccgtgc ggggggcggc
     1021 gggcgggggc acttccgccg cgggacggac gggcggaatc ctgacgatgt cgcccagcat
     1081 gacgcgctgc tcgggtcggg cccgcttctt gttgacccgg acctcgccct tgcgcagcag
     1141 ccggaaaacg tgggtccggg gaacgtcctt caggtggcgg accaggaagt tgtccagccg
     1201 cgtccctgca tcgtcgacgg tcacctcgac gtgccggaca cctcgtccgg ggccgtcggg
     1261 accgcgatcc gtgaccggcc ttgcctcatt ttcgtaagcc attgattatc cggacttcgc
     1321 tctgttagag tccgccgcga agcgcagtgc tggtgcgtgt aaacaacacc gcgccgcgcc
     1381 gaatgagcga cgcgtcgctg ctcacccgaa gggttctgcg gggcgcgcgc atcttagttg
     1441 gttctgagaa ttcagaccat cgatctgccg tgaacgacac gcaggtgcca agccccggcg
     1501 ggccttggac caggcgcgac tccggcgggt acgatgccca cagcaccagg atccggtcag
     1561 tcaccgcgcg ccacgcgcgg gtgcaccaga aaacgcagaa atgagcgctc gcgctgcaga
     1621 cgcgtcccga cctcctccca acgccaaggc accactaccc gtcctcgttc acggccccca
     1681 cctccagtaa agagagggcc tgtgaagaga atgctcgtca acgcaactca ggaagaagag
     1741 ttgcgcgtag ccctggtcga cggccagcgg atctacgacc tcgacatcga aatcccgtca
     1801 cgcgaaacca agaaagccaa cgtctacaag ggccgcatca cccgcgtcga gccgagcctc
     1861 gaggccgcgt tcgtcgacta cggcgccgag cgccacgggt tcctgccgct caaggaagtc
     1921 tccaagctct acttcaagga aaagcagccc ggccaggacg gcggccgcgg catccgcgac
     1981 ctgatcgagg aaggccagga aatcatcgtc caggtcgaga aggaggaacg cggcaacaag
     2041 ggcgccgcgc tcacgacctt catcagcctc gctggtcgct tcctggtgct gatgccgaac
     2101 aacccgcgcg cgggcggggt ctcgcgccgg atcgaaggcg acgaccgcga gcagctgcgc
     2161 gaggccatgg acgcgctgca gatcccggac gggatgggcg ccatcgtccg caccgcgggc
     2221 gtcggccggt ccccggaaga gctgcagtgg gacctcaaca acctggtcga cgtctggaac
     2281 gcgatccagg cggcgtcgga cagccgacct tcgccgttcc tgatctatcg cgagagcacc
     2341 gcgatcctcc gggcgctgcg cgactacctg gccgacgaca tcggcgagat cctggtcgac
     2401 aagcatgagg tgttcgagga agcgcgcgag tacatgcagc gcttcatgcc gaacaacctg
     2461 cgccggctca agctgtacga cgaccacgtg ccgctgttca cgcgctacca gatcgagagc
     2521 cagatcgaat cggcctacag ccacaaggtg acactgccgg cgggcggctc gatcgtgatc
     2581 gaccacaccg aggcgctcgt ctcgatcgac atcaattcgg cccgcagcac ccgcggcacc
     2641 gacatcgaga ccaccgcgct caacaccaac ctggaggcgg ccgacgagat cgcccgccag
     2701 ctgcggctcc gtgacgtggg cgggctgatc gtcatcgact tcatcgacat ggagtcgcag
     2761 aagaaccagc gcgccgtcga ggatcgcctg cgcgacgccg tcaagcagga ccgcgcgcgc
     2821 atccagctcg ggcgcatctc ccgcttcggc ctgctggagc tttcccggca gcgcctgcgg
     2881 ccgtcgatca gcgaatcgac caacatcgtc tgcccgcgct gcaacggcat gggcgtgatc
     2941 cgcagcgtcg agtcgctggc cctcgccgtg ctgcgcctga tcggcgagga agcgcgcaag
     3001 gaccgcaccg ccaaggtgat cgcgcagctg ccggtcgacg tcgcgaccta cctgatgaac
     3061 gagaagcgcg actggctgca cgacatcgag aaccgcagcc gcgtcgacat cgtgctggtg
     3121 ccgaacaagt atctcgaaac cccggcctac gagctgcggc gagtccgcga cgacgagatc
     3181 tcgctgcccg agaactcgat gatcagccac cagatggcca tcgagcccca gatcgacctc
     3241 gacgcgatcg cgcaggcgga agaggaaaaa gcgccgccgc cgacgccggc tgcggtgacg
     3301 tcgatcgtgc cgagcacgcc cgcaccgccg gccgcacccg agccggtgga accgccgaca
     3361 tccgcgccgg cgccgatcgc cgccgccgcg gccgttgcag cggcaccgct gtacggtccc
     3421 cgcgacaacg tgctcgtgcg gctctggcgc tggatcgccg gtgacaagcg cgcgcccgaa
     3481 ccggagcccg cgccgatccg caccgcccgc cccgagggcg atcgcgatcg ccaccggcgc
     3541 gaccgtgacg agcgcggacg cggccgtgac cgcgatgcgc gtcgcgacgg acgccgtgac
     3601 gaacgcgacg ggcgtcgtga cgctcgccg
//
LOCUS       DASPFR010000214        11107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_61540, whole genome shotgun sequence.
ACCESSION   DASPFR010000214 DASPFR010000000
VERSION     DASPFR010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11107
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61540"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            408..596
                     /locus_tag="VD737_05445"
     CDS             408..596
                     /locus_tag="VD737_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10039.1"
                     /translation="MARPTTLVFAREQARPAQMGMRVYWLVVTALSLTLLGMTVHVAT
                     PRDAAELAEAAATVAPVR"
     gene            complement(604..1986)
                     /locus_tag="VD737_05450"
     CDS             complement(604..1986)
                     /locus_tag="VD737_05450"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXW10040.1"
                     /translation="MGLWLRIGAFGLLLLGGVGAPQAASRMQGDLPRQADLGFTTTTG
                     DGRLEVAKVVPDGPAARAGLLVGDRIVEVDGESFAKPYHAAAALQRADGGRRIALTVT
                     RNDVRRKISFTPPPRPLEDVAGLDSLYDVVTTADGTRLRTIVTRPAGTNGRLPVIFFT
                     QWVSCGSLEFGQGGLSREILKRLALESGAALIRVDRAGSGDSEGPACHELDYDTELAH
                     YRSALDATLARHDWLDRDRLVIYGSSLGATLAPLVAQGRKVAGVMVQGGGAVTYLERM
                     IAFDRQQLERTDVPVADIHDRMLRQIAFHVEYLLRGRDPDQIAADGADMAQARASIRG
                     LGDGEHYGRPYDWHRQAARHDFLEAWSSIDAPVLVIYGEYDQFESRHGHELIADVING
                     RAPGRANFLAIPRMDHEGDVYDTIVDAYAWERPVSGPPETAARLQTGPMLRWLRDVVG
                     IAPIAPAAAK"
     gene            2168..4639
                     /locus_tag="VD737_05455"
     CDS             2168..4639
                     /locus_tag="VD737_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXW10041.1"
                     /translation="MDALVKAVAALAAARTVEEVMGVVRNSARSLTGADGVTFVLRDG
                     QSVFYADEDAIAPLWKGSRFPLEQCVSGLAMLKGEVLSIPDIYVDPRVPVDAYHPTFV
                     RSLLMTPIGRDESIAAIGAYWATVHEATTRERQNVQALAEAASLAFRNISLDGRLQDA
                     LTRERDARLAAERASLEKDESYQSLRRTEARLRKFIDAAPVGFAISEPDGSILLANDA
                     LLDMVGCTREEFERGLLDWQKFTPAEYLPLDMAHMEELRRGGTPPPFEKEFLRRDGNR
                     VSVLIVARFLPEEGERMVAYALDITESKRLARELEESNSRFDTLANASPVLLWVNGGS
                     GNQYVNRAYVDFVGAREEELLGDGWQKFVHPDDLNGYFAAYLEVASRGDPFDGTFRLR
                     RHDGEWRWMQSRGQPLLDPRGAVQGYAGSSQDVTELVAAQQALREADRHKDEFLATLA
                     HELRNPLAPIRNAVHLLKLSSTTDPQVRAARGMIERQLHQMIRLVDDLMEVSRITLGQ
                     ITLQSEQLDLRDVLSGALESALPLVEASGHQIEVTLPPEPVPVQGDLTRLSQVFQNLL
                     NNASKYTPRGGEIGLRLETDGMEAVVVVRDNGVGVPHAMQSRIFELFTRSHPTDEIKA
                     SGLGIGLALSRQLTELHGGRIEMRSDGPGTGAEFRVRLPMYAPVRRAASAAATPALEA
                     ATAAGRNGRRILVVDDNRDAAESLSMFLQIAGYEVDTAFDGIEGLQRFESLVPDIVLL
                     DIGMPQLDGYEVARRIRATPAGDDVLLVALTGWGQDKDRSLAQSAGFDEHMTKPVDPD
                     VLTMLLAAHGRQRVRGGAGRPRDSA"
     gene            complement(4731..6209)
                     /locus_tag="VD737_05460"
     CDS             complement(4731..6209)
                     /locus_tag="VD737_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXW10042.1"
                     /translation="MDRRQFCHSALAGSLTAAVPGLIGWYNSAIAATEADTSIPAVSL
                     DGKEIELERAAIRELGERLTGPVLLAGHPQYDAARVVWNGMHDKRPALIARCLTDQDV
                     RHAVTFAHERRLLTAVRGGGHSWPGKSVCDRGLMIDLSQMNAVTIDPAGRRATVRGGA
                     LLSMLDNQALQHGLATTTGVISHTGVGGYTLGGGYGRLNRKYGLTVDNLLGARIVTAD
                     GRLRRVSAEEEPDLFWAIRGGGGNFGVVTEFDYRLHPFDRKALCGSLVWPLAQARDVL
                     EFYAEWAPRLSDEMYAGPLLLRHPEAGDVVVMDVLYVGDPAAGEKELAPLRRIGKPTA
                     DAVVVQDYMIVQTENDGVFHPGVRSYAKNLLVKEWSQKLVDALVGAYDPRVLLANHVA
                     GGAVKRVGELATAFPHRQAEIMFVVVTTWEDRAKDDEMIAATRAYYDALKPFMGGYYD
                     NIEFDRKEGTETYGPALPRLAKIKRTFDPDNLFRLNSNILPA"
     gene            6681..7847
                     /locus_tag="VD737_05465"
     CDS             6681..7847
                     /locus_tag="VD737_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HXW10043.1"
                     /translation="MDERLRFVARLLEGEKMAPVCREFGISRVTGYKIFERYKACGLD
                     GLYDRSKAPYRQANKLPFQVERAILGIKKEFPSWGAPKIRDKLIREYPMIPPPAVGTI
                     HAALDRHGLVKRRKHRRHKACGTPLSAAHVPNGLWCADYKGEFLLGNRQYCYPLTITD
                     YRSRYLLACEGLESTKSRFAFSVFERTFRDFGLPAAIRTDNGVPFAAPWALFGLSRLA
                     VWWLRLGIRLQRIKPGQPQQNGRHERMHLTLKNETTKPAAFNALQQQERFEDFIRVYN
                     QERPHQALGGAYPAEVYTPSARPYEPPQEPAYPFHDRTVRVTRCGRICLGSRKINLST
                     ALAEQLVGIREVDDQVWQVSFLEYDLGYFDRDQDRVEPGPNPFAPDTVLTMCPE"
     gene            complement(7885..7961)
                     /locus_tag="VD737_05470"
     tRNA            complement(7885..7961)
                     /locus_tag="VD737_05470"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7925..7927),aa:Arg,seq:acg)
     gene            complement(7982..8072)
                     /locus_tag="VD737_05475"
     tRNA            complement(7982..8072)
                     /locus_tag="VD737_05475"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8036..8038),aa:Ser,seq:gct)
     gene            complement(8360..8524)
                     /gene="csrA"
                     /locus_tag="VD737_05480"
                     /pseudo
     CDS             complement(8360..8524)
                     /gene="csrA"
                     /locus_tag="VD737_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
     gene            complement(8829..>11107)
                     /gene="alaS"
                     /locus_tag="VD737_05485"
     CDS             complement(8829..>11107)
                     /gene="alaS"
                     /locus_tag="VD737_05485"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HXW10044.1"
                     /translation="TGTLGIAPERLWVTVYEHDHGAAEIWLKQIGVDPERFTRMGEKS
                     NFWAMGDTGPCGPCSEIFYDHGPEVPGGPPGSPDEDGDRYVEIWNLVFMQFNRAADGT
                     MTPLPKPSVDTGMGLERVAAVMQGVHSNYDIDLFRSLIEAAAKATGTSDLASNSLRVI
                     ADHVRAVSFLIADGVLPSNEGRGYVLRRIMRRAIRHGFMLGQRQPFFHTLVAALEQEM
                     GAAYPELASQRLHIEKVILQEEERFAETLAQGMGLLDEAIASLGGRKQIPGETVFRLY
                     DTFGFPVDLTSDIAREQGLTIDEAGFEAAMQAQRTRARAASKFNVDLRGGAMTVETQT
                     SFVGYDGEHGVGRVVSLFKDRQPVAKLEAGEHGQVVLDVTPFYAESGGQVGDQGALTG
                     TGVRFVVEDTQKLGKAHVHVGTLEQGTLAVGDTVEAAVDGERRQATRLNHTATHLLHA
                     ALRKVLGTHVQQKGSLVAPDRLRFDFSHYSAVTPQELARIESLVNEEIRRNADAEITL
                     MEFDAAVAAGAMALFGEKYDDEVRVLRIGDFSTELCGGTHAKRAGDIGFFKVVSEGGV
                     AAGVRRIEAVTGRGAYDYVVATEQKLRDVAGLVRGSRDDVDDKVRQLIDRARRLEKEV
                     AQLKERLASGGGTRDLASEARAIGGAGVKLVAASIEGADAAALRNTVDQLKSKLGSGI
                     IVLGSAGGGDKVMLIAGVTNDLTTRVKAGEIVNYVAKQVGGKGGGRADLAQAGGTQPE
                     NLQPALDSLAAWLAPQLA"
BASE COUNT         1750 a         3831 c         3695 g         1831 t
ORIGIN      
        1 gcaaggccaa ggacgcgccc ccctcggccg ggcgggtgac cgaccagacc gacgaacgca
       61 ggaagaaatc ctccgccaag cgcgctcgcc accggccggc gcctgcggag ggcgccgagt
      121 cgaactgacg ccatgccggg ccgcggacga tccgctctcg tggcggtccg cggcccggcg
      181 acggccctcg cgccgatgcc gccggcaagc ccaagtgcca gttccgactg gggattttgt
      241 caggcgaggt gaactggctg accgcggtcg ttcccgccgt cccacagcgc aggactccat
      301 ctcgacgctc gtctcgtcct gccggcgccc gtgccggcgc gtcgtcgtcc gctgcgcctc
      361 gcgggcgcgc ccggttcatc ctgcggtcaa ggcatggagg attcggtatg gcaaggccaa
      421 cgacgctcgt attcgcccgc gagcaggcac gccccgccca gatgggcatg cgcgtgtact
      481 ggctcgtcgt gacggcgctg tccctcacgc tgctcggcat gacggtgcac gtggcgacgc
      541 cgcgcgatgc ggccgaactc gcggaagccg cggccaccgt cgcgccggtg agataacggc
      601 cgatcacttc gccgcggcgg gcgcgatcgg cgcaatgccg accacgtctc tcagccagcg
      661 cagcatcggc ccggtctgca acctcgcggc cgtctcgggc ggcccgctga ccgggcgctc
      721 ccaggcgtag gcgtccacga tcgtgtcgta cacgtcgccc tcgtggtcca tccgcggaat
      781 cgccaggaag ttggctcggc ccggcgcgcg tccattgatg acgtccgcga tcagttcgtg
      841 gccgtggcgg ctttcgaact ggtcgtactc tccgtagatc accaggaccg gcgcgtcgat
      901 cgacgaccac gcctcgagga agtcatgccg ggcagcctgg cggtgccagt cgtacggccg
      961 accatagtgt tcgccgtcgc ccagcccgcg aatcgaggcg cgggcctgcg ccatgtccgc
     1021 gccatccgcc gcgatctggt ccggatcccg cccacgcagc agatattcga cgtggaacgc
     1081 gatctgccgc agcatccggt cgtggatgtc ggcgacgggg acgtcggtgc gttcgagttg
     1141 ctggcgatcg aacgcgatca tgcgctcgag gtaggtcacc gcgccgccgc cctggaccat
     1201 cacgcccgcc accttgcgac cctgcgcgac gagcggcgcc agggtcgcgc cgagactcga
     1261 gccgtagatc acgagccggt cgcgatcgag ccagtcgtgg cgggcgagcg tcgcatcgag
     1321 cgcgctgcgg tagtgcgcga gttcggtgtc gtagtcgagc tcgtggcagg ccgggccctc
     1381 actgtcgcca ctgcccgcgc gatcgacacg gatcaacgcc gcgccggact ccagcgcgag
     1441 tcgcttgagg atctcgcgcg acagcccgcc ttggccgaac tcgagcgagc cgcaactcac
     1501 ccactgcgtg aagaagatga ccggcagtcg tccattcgtg ccggccggcc gggtgacgat
     1561 cgtccgcaac cgggtcccgt ccgcggtggt cacgacgtcg tacaggctgt cgaggcctgc
     1621 cacgtcctcg agcggccgcg gtgggggcgt gaacgagatc ttgcgacgga cgtcattgcg
     1681 cgtgacggtg agtgcgatcc gccgtccgcc gtccgctcgc tggagggccg cggcggcgtg
     1741 ataaggcttt gcgaacgact cgccgtcgac ctcgacgatg cggtcgccga cgagcagtcc
     1801 ggcccgggcg gctggaccgt cggggacgac cttggcgacc tcgaggcgac cgtcgccggt
     1861 ggtggtcgtg aagcccaggt cggcctggcg cggcaagtcg ccctgcatcc gactggccgc
     1921 ctgcggcgcg ccgacgccac cgagcagcag caggccgaac gctccgatac gcagccataa
     1981 gcccatgggg cggagcgttg cagaacgggc cgaccgcggg aatgcgcgtt gcgatcaggc
     2041 gcccgggtcg tgcgccaggc cgtggccgag acacctgtcc gacagcggtc gcgccaccgg
     2101 ttccaaactc gtggcgcaga ccggacccat cgctaaaatg ccggcactct ggctcgtttg
     2161 gtaacacatg gacgcgttgg tcaaggccgt tgccgcgctg gccgctgctc ggaccgtcga
     2221 agaggtcatg ggcgtcgtgc gcaacagcgc acgctcgctc actggagccg acggtgtcac
     2281 gttcgtgctg cgcgacggcc agagcgtctt ctacgccgac gaggacgcga tcgctccgct
     2341 gtggaaaggc agccgctttc ccctggaaca gtgcgtctcg gggctcgcga tgctgaaggg
     2401 cgaggtcctc tccatccccg acatctacgt cgatccccgc gtgccggtcg acgcctacca
     2461 cccgacgttc gtgcggagcc tgctgatgac gccgatcggt cgcgacgagt cgatcgccgc
     2521 gatcggtgcg tactgggcca cggtgcacga agcgacgacg cgcgagcggc agaacgtaca
     2581 ggcgctcgcg gaggcggcat cgctggcgtt ccgcaacatc agcctcgacg gtcgcctgca
     2641 ggacgcgctg acccgcgagc gggacgcgcg gctcgcggcc gagcgggcga gcctcgagaa
     2701 ggacgagtcg taccagagcc tgcgtcgtac cgaggccagg ctgcgcaagt tcatcgacgc
     2761 cgcgccggtc ggcttcgcga tcagtgaacc cgacggaagc atcctgctgg ccaacgacgc
     2821 gctgctggac atggtcggct gcacgcgcga ggagttcgag cgcggattgc tcgactggca
     2881 gaagttcacg cccgcggaat acctgcccct cgacatggcg cacatggagg agctgcgtcg
     2941 agggggcacg ccgccgccct tcgagaagga gttcctgcgc cgtgacggca accgtgtctc
     3001 cgtactgatc gtcgcccggt tcctgcccga ggagggcgag cgcatggtcg cctatgcgct
     3061 cgacatcacc gaaagcaagc ggctcgcgcg cgagctcgag gagtccaaca gtcgcttcga
     3121 cacgctcgcc aacgccagcc cggtgctgct ctgggtcaac ggtggctcgg gcaatcaata
     3181 cgtgaatcgc gcctatgtcg acttcgtcgg tgcgcgcgag gaggaactgc tcggcgacgg
     3241 ttggcagaag ttcgtgcacc cggacgacct gaacggctac tttgccgcct acctcgaagt
     3301 cgcaagtcgc ggcgatccgt tcgacggcac gtttcgcctg cgtcggcacg acggcgagtg
     3361 gcgctggatg cagtcgcgcg gccagcccct gctcgacccc aggggtgccg tgcagggata
     3421 cgccggcagc agccaggacg tcacggagct ggttgcggcc cagcaggcct tgcgcgaggc
     3481 ggaccgtcac aaggacgaat tcctcgcgac gctcgcgcac gagcttcgca acccgctggc
     3541 gccgatccgc aatgccgtgc acctgctcaa gctgtcgagc acgaccgacc cgcaggtgcg
     3601 cgccgctcgc ggcatgatcg agcgccagct gcaccagatg atccggctcg tcgacgacct
     3661 gatggaggtc tcgcgcatca ctctcgggca gatcacgctg cagtcggagc aactcgacct
     3721 ccgcgacgtg ctcagtggtg cgctggaaag cgcgctgccg ctcgtcgagg cctccggtca
     3781 ccagatcgaa gtgacgctgc cgccagagcc cgtgccggtg cagggcgacc tgacgcggct
     3841 gtcgcaggtc ttccagaatc ttctcaacaa cgccagcaag tacacgcccc gcggtggcga
     3901 gatcggcctg cgactggaga ccgatggcat ggaggcggtc gtcgtcgtgc gggacaacgg
     3961 agtcggcgtg ccgcacgcga tgcagtcgcg gatcttcgag ctcttcactc gcagccaccc
     4021 cacggacgag atcaaggcga gcggcctcgg gatcggcctg gcgctgtcgc ggcagttgac
     4081 tgagctgcac ggcggtcgta tcgagatgcg cagcgacggt ccgggtacgg gcgcggaatt
     4141 ccgcgtgcgg ttgccgatgt acgcgccggt tcgccgcgcc gcgtcggcag ccgcgacgcc
     4201 cgcattggag gcagccaccg cggccggtag gaacggtcgg cggatcctcg tcgtcgacga
     4261 caaccgcgac gcggccgaga gcctgtcgat gttcctgcag atcgcgggtt acgaggtgga
     4321 cacggcgttt gacggcatcg aaggactgca gcgcttcgag tcgctggtgc cggacatcgt
     4381 cctcctcgac atcggtatgc cgcaactcga cggttacgag gtggcgcggc gcatccgtgc
     4441 gacgcccgcc ggtgacgacg tgctgctggt cgccctcacc gggtggggcc aggacaagga
     4501 tcgctccctg gcgcagtcgg cgggcttcga cgagcacatg accaagcccg tggaccccga
     4561 cgtgctcacg atgctgctgg cggcgcacgg gcgccaaagg gtgcgcggcg gcgcggggag
     4621 gccgcgggat tcggcatgat ccggccggca cgccgccgtc gacacggcgg caaatgaaaa
     4681 aaagggccag ccgcaagctg gcccttttca cgacccgcca cggcgggtcg tcacgcgggc
     4741 aggatgttgc tgttcaaccg gaagaggttg tccggatcga acgtgcgctt gatcttcgcc
     4801 aggcgcggca gcgccggtcc gtaggtctcg gtcccttcct tgcggtcaaa ctcgatgttg
     4861 tcgtagtacc cgcccatgaa cggtttcagc gcgtcgtaat acgcgcgcgt ggcggcgatc
     4921 atctcgtcgt ccttcgcccg atcctcccag gtggtgacga ccacgaacat gatctcggcc
     4981 tggcgatgcg ggaacgccgt cgcgagctcg cccacgcgtt tcacggcccc acccgccacg
     5041 tggttcgcga gcagcacgcg cgggtcatag gcaccgacga gtgcgtccac gagcttctgc
     5101 gaccactctt tcaccagcag gttcttggca taggaccgca cgcccgggtg gaagacgccg
     5161 tcgttctcgg tctgcacgat catgtagtcc tgcaccacga ccgcgtccgc cgtgggcttg
     5221 ccgatccgcc gcaacggggc gagctctttc tcgccggcgg cgggatcgcc cacgtacagg
     5281 acgtccatga cgaccacgtc gcccgcttcg ggatggcgca gcagcagcgg gccggcatac
     5341 atctcgtcgg agagacgcgg tgcccactcc gcgtagaact cgaggacgtc ccgtgcctgc
     5401 gcgagcggcc agacgaggct tccgcacagc gccttgcggt cgaacgggtg cagccggtag
     5461 tcgaactcgg tgacgacccc gaaattgcca ccgcccccgc ggatcgccca gaacaggtcg
     5521 ggctcttcct ccgcgctcac ccgccgcagc cgcccgtccg ccgtgacgat ccgcgcgccg
     5581 agcaggttgt ccacggtgag gccgtacttg cggttgagcc tgccgtagcc gccgccgagc
     5641 gtgtacccgc cgactccggt gtgcgagatc acacccgtcg tcgtcgcgag cccgtgctgc
     5701 aacgcctggt tgtcgagcat gctcagcagg gcgccgcccc gcacggtggc gcggcgcccg
     5761 gccgggtcga tcgtgaccgc gttcatctgc gagaggtcga tcatcaggcc gcggtcgcag
     5821 acggatttgc ccggccagct gtggccgccg ccgcggaccg cggtcagcag ccgacgttcg
     5881 tgcgcgaacg tgaccgcgtg cctcacgtcc tggtcggtca ggcatcgcgc gatcagcgcc
     5941 ggccgcttgt catgcatgcc gttccagacg acgcgggcgg catcgtattg cggatgcccg
     6001 gccagcagca ccgggccggt gagtcgttcg ccgagttcac ggatcgcggc ccgctcgagt
     6061 tcgatttcct tgccatcgag tgagacggcc gggatgctcg tatcggcttc ggtcgccgcg
     6121 attgcagagt tgtaccaacc aatcaatccg ggtacggccg cagtgaggct gccggccaat
     6181 gcgctgtggc aaaactgacg cctgtccatg gagtttgttc cccccggcga gttgtttgaa
     6241 atgcgtgcgt gcgccgcatt aacttactca cgaggcaggg cggtttcaac agggagcccg
     6301 tggcccaaag ttcgagcttg cgttattcca cctcgaacca agatcggttg atattctttc
     6361 cgtgagctct cgtgacggtt gtggctgcgg cgaatcggcc tcggcgctgg cgactggaca
     6421 gaacgtcgct cgccgccgcg cgctgtggat cttcccgacg atcatcggga cgaggaccgg
     6481 gtggaaccag gtcgcaatcc tttcgccccg gacacagtgt taaccatgtg tccggaatga
     6541 gatgtaaacc gtgtgaccgg aatacggaat acaccggtga gcttatcgac agatagcttg
     6601 caggttattc cgagcacatg gtttacacat ttccgcattt gggggagcgc tcccagatgc
     6661 cctggaaaga gtgtaagccg atggatgaac gcctccgctt cgtagcccgc ctgctggaag
     6721 gcgagaagat ggcgccggtg tgccgcgagt tcggcatctc ccgggtgacc ggctacaaga
     6781 tcttcgagcg ctacaaggcc tgcggcctcg acgggctcta cgaccgcagc aaggctccgt
     6841 accgccaggc caacaagctg ccgttccagg tcgagcgggc gatcctcggc atcaaaaagg
     6901 agttcccgtc ctggggcgcg cctaagatcc gcgacaagct gatccgcgag tacccgatga
     6961 tcccgccgcc ggctgtgggc acgatccacg cggcgctcga tcgccacggg ctggtcaaac
     7021 gccgcaagca ccgccgccac aaggcgtgcg gcacgccgct gtccgcggcc cacgtgccca
     7081 atggcctctg gtgcgccgac tacaagggtg agttcctgct cggcaaccgg cagtactgct
     7141 acccgctcac catcaccgat taccgctccc gctacctgct cgcctgcgag ggcctcgagt
     7201 cgaccaagtc ccgcttcgcc ttctcggtgt tcgagcgcac cttccgcgac tttggcctcc
     7261 cggccgcgat ccgcaccgac aacggcgtcc cgttcgccgc gccctgggcg ttgttcggct
     7321 tgtcccgcct cgcggtgtgg tggctgcgcc tggggatccg cctgcaacgc atcaagcccg
     7381 gccaaccgca gcagaacggc cgtcacgagc gcatgcacct gacgctcaag aacgaaacga
     7441 ccaagcccgc cgccttcaac gccctgcagc agcaggagcg cttcgaggac ttcatccggg
     7501 tttacaacca ggagcggccg caccaggcgc tcggcggcgc ctatccggcc gaggtctata
     7561 caccttcagc ccgcccctac gagccgcccc aggaacccgc gtacccgttc cacgaccgca
     7621 ccgtgcgcgt gacgcgctgc ggacgcatct gcctcggcag tcgcaagatc aacttgagta
     7681 cggcgctggc ggagcagctg gtgggcatcc gcgaagtcga cgaccaggtc tggcaggtca
     7741 gcttcctgga gtacgatctt ggatacttcg atcgagatca ggaccgggtc gagcccggtc
     7801 ccaatccgtt cgccccggac acagtgttaa ccatgtgccc ggaatgagat gtaaaccgtg
     7861 tgaccggaat acaccttatt tcactggcgc gcccggaagg attcgaacct ccgaccttct
     7921 ggttcgtagc cagacgctct atccaactga gctacgggcg cgtgattctt ctttcgacaa
     7981 ctggcggaga gagagggatt cgaaccctcg aagggctttt gacccttacg cccttagcag
     8041 gggcgcgcct tcgaccactc ggccatctct cctgaaaacg gagagccgcg aaggataaac
     8101 gccggaacgc gccccgtcca gcctgaggcg ggtccgggac gttgcgcttg acagtgtggc
     8161 caggatggcg agcgcgactc gccgggctgc cggagtcagc gaggccgatc gaaccgatgg
     8221 atcgacttcg cccggcgata tatcaatacg tgtcgcgatc gatgccgttg gtcgcgtgac
     8281 cgttgccgcg ggccgaggca cgcgtggtcg acgtcgtggt ggtgggggtg gtggtcgtgc
     8341 cggcggtgct gccgtcgtgc tcctcacgct tgatgcgctc gaagatctct tcgcgatgca
     8401 cggcgacgtc gcgcggcgca ttgacgccca cgcgcacctg gttgcccttg acgcccagta
     8461 cggtcacggt cacgtcgttg ccgatcatga ctgtctcgcc gacacgccga gtgaggatca
     8521 acatcttggt tttctcctag cctgttggct tgtacttccg ccggacacga gtccattcca
     8581 tcccacagcg ttttccggcc aggaccgggg agactcgacc gccgagcgac actcggcgca
     8641 ctcctgctgc accccactga acgtaagcac gggacttgga ttcctggtgc cgccgttcga
     8701 cgcaccaaaa gatagcgcgg agccggtggg ttgtcagcaa cgaccgacaa cgaaggattc
     8761 gttttggtat tactacgtat cgcccgtcgt cgcgatcggg caacctgcga gcgactttgg
     8821 gccacgcgtc aggcgagttg tggcgccagc caggccgcga gactgtccag cgccggctgc
     8881 aggttttcgg gctgcgtgcc cccggcctgg gcgaggtcgg cgcgaccgcc tcccttgccg
     8941 ccgacctgtt ttgcgacgta gttcacgatt tcaccggcct tgacccgcgt cgtcaggtcg
     9001 ttggtcacgc cggcgatcag catgaccttg tcaccgccgc ccgcggatcc gagcacgatg
     9061 atgccactgc cgagcttgct tttgagctga tcgacggtgt tgcgcagcgc cgcggcatcg
     9121 gcgccctcga tgctggccgc gacgagcttc acgcccgcac cgccgatcgc cctggcctcc
     9181 gacgcgagat cgcgcgtccc accaccgctc gcgagccgct ccttgagctg ggcgacctct
     9241 ttttcgaggc ggcgcgcgcg atcgatcagt tgccgtacct tgtcgtcgac gtcgtcgcgc
     9301 gagccgcgca cgagtcccgc gacgtcgcgc agcttctgct ccgtcgcgac gacgtagtcg
     9361 taggcgcccc gccccgtcac ggcctcgatg cgccgaacgc cggccgcgac gccaccttcg
     9421 ctgacgacct tgaagaaccc gatgtcgccc gcgcgcttcg cgtgggtgcc gccgcacagt
     9481 tcggtcgaga agtcgccgat gcgcaggacg cgaacctcgt cgtcatactt ctcgccgaac
     9541 agcgccatcg cgccggccgc gaccgccgcg tcgaactcca tgagggtgat ctcggcgtcg
     9601 gcgttgcgcc ggatttcctc gttcacgagc gactcgatcc tggcgagctc ctgcggggtg
     9661 accgccgagt agtgcgaaaa gtcgaaacgc agccggtccg gtgccacgag cgagcccttc
     9721 tgctgcacgt gggtgccgag caccttgcgg agcgccgcgt gcagcaggtg cgtcgccgtg
     9781 tgattgaggc gcgtcgcctg gcgacgctcg ccgtccacgg cggcttcgac cgtatcgccc
     9841 accgcgagcg tgccctgctc gagcgtgccg acgtgcacgt gcgctttgcc gagcttctgc
     9901 gtgtcctcga cgacgaagcg cacgcccgtc ccggtgagcg ccccttggtc gccgacctgt
     9961 ccaccgcttt ccgcgtagaa cggcgtcacg tcgagcacga cctgtccgtg ttcgccggcc
    10021 tcgagcttcg cgacgggctg ccggtccttg aacaaggaca cgacccgccc gacgccgtgc
    10081 tcgccgtcgt acccgacgaa cgacgtctgc gtttcgaccg tcatggcgcc gccgcgcagg
    10141 tcgacgttga acttgctcgc cgcgcgcgca cgcgtgcgct gggcctgcat tgccgcctcg
    10201 aaaccggcct cgtcgatcgt cagcccctgc tcgcgcgcga tgtcgctcgt gaggtcgacc
    10261 gggaacccga acgtgtcgta cagacggaac accgtctcgc ccgggatctg cttacggccg
    10321 ccgagcgagg cgatcgcctc gtcgagcaat cccatgccct gcgcgagcgt ttcggcgaag
    10381 cgctcctcct cctgcaggat gaccttctcg atgtgcagcc gctgcgaggc gagttcgggg
    10441 tacgcggcgc ccatttcctg ctcgagcgcc gcgaccagcg tgtggaagaa cggctgtcgc
    10501 tggccgagca tgaagccgtg ccggatcgcg cgccgcatga tccgccgcag cacgtagcca
    10561 cggccttcgt tggacggcag cacgccgtcg gcgatcagga acgaaacggc gcgcacgtgg
    10621 tcggcgatga cccgcagcga gttcgacgcg aggtcgctcg tgccggtcgc cttcgcggcc
    10681 gcctcgatca ggctgcggaa caggtcgatg tcgtagttgc tgtgcacgcc ctgcatgacg
    10741 gccgcgacgc gctcgagtcc catgccggtg tcgacggaag gcttcggcaa cggcgtcatc
    10801 gtgccgtcgg ccgcgcggtt gaactgcatg aagaccaggt tccagatctc gacgtagcgg
    10861 tcgccgtcct cgtccggcga gcccggcggg ccgccaggca cctcagggcc gtggtcgtag
    10921 aaaatctcgg agcacgggcc gcacgggccg gtgtcgccca tggcccagaa gttcgacttc
    10981 tcgcccatgc gcgtgaagcg ctcgggatcg acgccgatct gcttgagcca gatctcggcg
    11041 gcgccatggt cgtgctcgta gaccgtcacc cagagccgct cgggcgcaat gccgagcgtg
    11101 ccggtga
//
LOCUS       DASPFR010000215         5144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1648615, whole genome shotgun sequence.
ACCESSION   DASPFR010000215 DASPFR010000000
VERSION     DASPFR010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1648615"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /locus_tag="VD737_05490"
     CDS             complement(<1..1490)
                     /locus_tag="VD737_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXW10045.1"
                     /translation="MDFSRFFIDRPIFAAVLSIVIFAAGLIAIPLLPVTEYPDVVPPT
                     VQVRAVYPGANPKVIAETVATPIEEAINGVENMLYVKSVAGTDGVLVTTVTFRAGTDP
                     DDAQVLVQNRVNQALARLPEDVRRQGVVTQKQSPTLTMVVHLLSPDGRYDSLYLRNYA
                     TLRIKDELARLPGVGEVGLFGSGDYAMRVWLDPDKVAARGLTAGDVVRAIREQNVQVS
                     AGQLGAEPTPNGSDFLVSINAQGRLETEEEFGQVVIDSGTDGEIVRLADVARLELGAG
                     DYTLRSQLDNKDAVAIPIFEAPGANAIELSDAVRTKMAELATRFPEGVTYDVVYDPTV
                     FVRDSIKAVVTTLFEAVLLVVLVVILFLQTWRASVIPLLAVPVSIVGTFAALHLLGFS
                     INTLSLFGLVLAIGIVVDDAIVVVENVERNIEEGLAPLAAAHQAMKEVSGPIIAIALV
                     LCAVFVPLAFVTGLTGQFYRQFAVTIAISTVISAINSLTLSPALSA"
     gene            complement(1501..2715)
                     /locus_tag="VD737_05495"
     CDS             complement(1501..2715)
                     /locus_tag="VD737_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXW10046.1"
                     /translation="MKPLPKKSLRAAVLVPLLVVAAAGVVLAVTRDGASNEAAAPPAP
                     AVSVAKVVTREFQPWDEFTGRIAAVETVELRPRVSGYIERVAYEEGREVRKGDLLFVI
                     DQRRYRAAYDRAVAELERARSEARLATGELARAETLLASRAISREEYDARRAAHTQGD
                     ASVRAAAAAVETARLDLQFTEVRSPIDGRAGRALVTVGNLAQADSSLLTTVVSLDPMY
                     VYFDADEHTFLDYANATRRGEHRTTSSPVRVGLANEPGFPHPGTVDFVDNQVDPRTGT
                     IRARAVVANADRSLTPGLFARVQLAGGNRQTALLIDEKAVLTDQDRKYVYVLGPNDVA
                     VRKDVVLGRRDGELRVVDRGLEPGETVIVHGVQKVFFAGMPVSPQEIVMGESAPDGLR
                     LAANRASTADAS"
     gene            complement(2727..3668)
                     /locus_tag="VD737_05500"
     CDS             complement(2727..3668)
                     /locus_tag="VD737_05500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXW10047.1"
                     /translation="MDTKDPRAVASKVGDQSDQPLTTETSQNEHDRPTDGAVLPAHGT
                     MAAPIVVVGATGTIGRAIVRTLVADGAPVVAVARRREKLEHLRDAATPGAIVPIVGDI
                     TSERDAEELVGRLRGLGRPFAGVVVAFPHGRSAEIGVDRSRLLDQSTADLADCLEQTL
                     LAQHALARHLIPLLAGSGRNARYVIVGGPGSETPWAGYGHRSVAMAATRMLVRVLHDE
                     ARAGGVRVQMVSIDSPVRNEQPGEHECPEWPTAGDVARRVAQLVDCTAQPSSTEAVVA
                     CARSQPTCSGGRIARAFGDVPSFLASLRNTTNKITPQ"
     gene            3793..4824
                     /locus_tag="VD737_05505"
     CDS             3793..4824
                     /locus_tag="VD737_05505"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXW10048.1"
                     /translation="MARDLNDTLIFAKVVEHGSFISAARALRLPKTTVSRKIQELESR
                     LGAQLLHRTTRKLGLTEAGNIYFEHSQRIAKELDEAESAIGQLQGGPRGWLRVTAPYS
                     FAISRITPLFGEFQARYPEVRVEMVLTNDRIDLIDKEIDVALRIGPLQDSNLVARRLA
                     VFRSQVYASPRYIERHGEPVHPADLLHHRTLAMNKYGLIGNGGYYWPLNDGTTTTDFR
                     IHPVLVANDPAALHPALLTGEGLMLTADVMVKAYAEHGYIQRVLAGWTGPSAEMNAVF
                     PRGQGKSPKVRAFVDFLIERVKFDADYMDELCPNHQCCEAEMRVAEATATLGIDAVVQ
                     VPTPVLAEA"
     gene            4848..>5144
                     /locus_tag="VD737_05510"
     CDS             4848..>5144
                     /locus_tag="VD737_05510"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HXW10049.1"
                     /translation="MQPETVLEFWFDALEPDQWWSVDPQLDATIRDRFLPLWHRAIAG
                     ELYPWRGTAPGRVAEVIVLDQFSRNVFRDTPAAYAQDPIALVLAQEAVASGALEA"
BASE COUNT          811 a         1772 c         1785 g          776 t
ORIGIN      
        1 atcgcggata acgccggcga gagtgtgagc gaattgatcg ccgagatcac cgtcgagatc
       61 gcgatcgtca ccgcgaactg gcgatagaac tggccggtca gaccggtcac gaaagcgagc
      121 ggcacgaaca ccgcgcacag caccagcgcg atcgcgatga tgggcccgga gacttccttc
      181 atcgcctggt gcgcggccgc gagcggtgcc aggccttcct cgatgttgcg ctcgacgttc
      241 tcgaccacca cgatcgcatc gtcgacgacg atgccgatgg cgagcaccag cccgaacagg
      301 ctcagcgtgt tgatcgagaa gccgagcagg tgcagcgcgg cgaacgtgcc gacaatcgac
      361 accggcacgg cgagcagcgg gatcaccgag gcgcgccagg tctgcaggaa caggatcacg
      421 accagcacga ccagcagcac cgcctcgaac aacgtcgtca cgacggcctt gatcgagtcg
      481 cggacgaaca ccgtcgggtc gtagacgacg tcgtacgtga caccctcggg gaagcgcgtg
      541 gcaagctcgg ccatcttcgt gcgcaccgcg tccgacagct cgatcgcgtt ggcgcccggc
      601 gcctcgaaga tcgggatcgc gaccgcgtcc ttgttgtcga gctgcgaacg cagcgtgtag
      661 tcgccggccc ccagctcgag ccgcgcgacg tcggccagcc gcacgatctc gccgtcagtg
      721 ccgctgtcga tcacgacctg gccgaactcc tcctcggtct cgagccggcc ctgcgcattg
      781 atggagacca ggaagtcgct gccgttcggc gtgggttccg cgccgagctg gccggccgac
      841 acctgcacgt tctgttcgcg gatcgcgcgg acgacgtcgc cggccgtgag cccgcgggcc
      901 gcgaccttgt cgggatcgag ccacacgcgc atcgcgtagt cgcccgagcc gaacagtccg
      961 acttcgccga ccccgggcag ccgcgcgagc tcgtccttga tcctgagcgt cgcgtagttg
     1021 cgcaggtaca gcgagtcgta gcggccgtcc ggcgacagca ggtgcacgac catcgtcagc
     1081 gtcggtgact gcttctgcgt gacgacgccc tggcgccgga cgtcctcggg cagtcgcgcg
     1141 agcgcctggt tgacccggtt ctgcaccagc acctgcgcgt cgtccgggtc ggtgcccgcg
     1201 cggaacgtga cggtggtgac cagcacgccg tcggtgccgg cgaccgactt cacgtacagc
     1261 atgttctcga cgccgttgat ggcctcctcg atcggcgtcg ccaccgtctc ggcgatcact
     1321 ttcgggttcg cgccgggata cacggcccga acctggaccg tcggcggcac gacgtcgggg
     1381 tactccgtga ccggcagcag cgggatcgcg atcaacccgg ccgcgaagat cacgatcgac
     1441 agcacggccg cgaagatcgg ccggtcgatg aagaacctgg agaagtccat ggcttgatcc
     1501 tcagctcgcg tcggccgtcg aggcccggtt ggctgccagt cgcaggccgt ccggcgccga
     1561 ctcgcccatg acgatttcct ggggtgacac cggcatgccg gcgaagaaca ccttctgcac
     1621 gccgtggacg atgacggtct cgccaggctc gaggccgcga tcgaccacgc gcagttctcc
     1681 gtccctgcgg ccgagcacga cgtcctttcg cacggcaacg tcgttggggc cgagcacgta
     1741 gacgtacttg cggtcctggt ccgtgaggac cgccttctcg tcgatcagca gcgccgtctg
     1801 gcggttgccg ccggccagct ggacgcgcgc gaacaggccg ggcgtcaacg accggtcggc
     1861 gttcgcaacc acggcgcgcg cccggatggt cccggtgcga gggtcgacct ggttgtcgac
     1921 gaaatccacg gtgccggggt gcgggaagcc cggctcgttg gcgaggccga cgcgcacggg
     1981 gctggacgtc gtgcggtgct cgccgcgacg cgtcgcgttc gcgtagtcga ggaacgtgtg
     2041 ttcgtcggcg tcgaagtaga cgtacatcgg gtccaacgac acgacggtcg tgagcaggct
     2101 cgagtccgcc tgcgcgagat tgccgacggt gaccagcgcc cggccggcgc ggccgtcgat
     2161 cggcgagcgc acctccgtga actgcaggtc gagcctggcc gtctcgaccg cggcggccgc
     2221 ggcacgcacg gaggcgtcgc cctgcgtgtg cgcggcgcgc cgggcgtcgt actcctcccg
     2281 cgagatcgcg cgcgaggcga gcagcgtctc ggcgcgcgcg agttcgccgg ttgcgagccg
     2341 ggcctcgctg cgcgcgcgct cgagctcggc gacggcacgg tcataggccg cgcggtaacg
     2401 gcgctggtcg atcacgaaca gcaggtcgcc cttgcgcacc tcgcggcctt cctcgtaggc
     2461 gacgcgctcg atgtagccgc tcacgcgcgg ccgaagctcc actgtctcga cggcggcgat
     2521 gcggcccgtg aactcgtccc acggctggaa ctcgcgcgtg accaccttgg cgacgctgac
     2581 ggcgggcgcg ggtggtgcgg cggcctcgtt cgatgcgccg tcgcgcgtga cggccagcac
     2641 gacgcccgcg gccgcgacca ccagcagggg cacgagcacg gccgctcgca gagacttctt
     2701 gggtaacggt ttcattgcaa aggtcctcac tgcggggtga tcttgttcgt ggtgttgcgt
     2761 agcgatgcga gaaacgacgg cacgtcgccg aacgcgcggg cgattcgccc gcccgagcac
     2821 gtcggctggc tgcgcgcgca ggcgacgacg gcctcggtgg acgaaggctg ggccgtgcaa
     2881 tcgaccagct gtgcgacgcg acgcgcgacg tcgcccgcgg tcggccactc cgggcattcg
     2941 tgctcgcccg gctgctcgtt gcggaccggg gagtcgatcg agaccatctg cacgcgcacg
     3001 ccgccggcgc gagcttcgtc gtgcagcacg cggacgagca ttcgcgtcgc cgccatcgcg
     3061 accgagcgat gaccgtagcc ggcccagggg gtttcgctgc cggggccgcc gacgatcacg
     3121 taacgggcgt tgcgacccga gccggcgagc aacggaatca ggtgtcgcgc cagcgcgtgc
     3181 tgcgccagca gcgtctgctc gaggcagtcg gccaggtcgg cggtggactg gtcgagcagc
     3241 cgactgcgat ccacgccgat ttccgcggag cggccgtgcg gaaacgcgac gacgacgccg
     3301 gcgaacggac gaccgaggcc gcgcaggcgc ccgacgagtt cctccgcgtc gcgctcggag
     3361 gtgatgtcac cgacgatcgg cacgatcgcg cccggggtgg cggcgtcgcg caggtgctcg
     3421 agtttttccc gtcggcgcgc cacggcgacg accggggccc cgtccgccac cagcgttcgc
     3481 acgatcgccc gtccaattgt cccggtggca ccaacgacga cgatcggggc agccattgtc
     3541 ccgtgggcgg gaagcaccgc cccatcggtg ggacgatcgt gctcattctg tgacgtttcc
     3601 gtcgtaagcg gttgatctga ctgatcgcct actttcgacg caacggctct cgggtccttg
     3661 gtgtccatgg gcgcgtacga taggcgcacc gatgcccgtt gattagccgt caatttaggg
     3721 aataattgtc ccgcctccgg tccaatccag tcttcgactc acggacggcg cgcggcacgc
     3781 aggagcacga cgatggcacg cgacctcaac gacacgttga tcttcgccaa ggtggtcgaa
     3841 cacggcagct tcatctccgc ggcgcgcgcg ttgcgactgc caaagaccac ggtgagccgc
     3901 aagatccagg aactcgaatc gcggctcggt gcgcaactgc tgcaccggac gacgcgcaag
     3961 ctcggtctca cggaagccgg caacatctac ttcgagcact cgcagcgcat cgcgaaggaa
     4021 ctggacgagg ccgagagcgc gatcggtcag ctgcagggcg ggccgcgggg ctggttgcgg
     4081 gtgacggcgc cctactcgtt cgcgatctcg cgcatcacgc cgctcttcgg cgagttccag
     4141 gcgcgctatc ccgaggtgcg cgtcgagatg gtgctgacca acgatcgcat cgacctgatc
     4201 gacaaggaaa tcgacgtcgc gctgcggatc gggccgctgc aggacagcaa cctcgtggcg
     4261 cgccggctcg cggtgtttcg cagccaggtc tacgcgagcc cgcgctacat cgagcgccac
     4321 ggcgagcccg tgcacccggc cgacctgctg caccatcgta cgctcgccat gaacaagtac
     4381 gggctgatcg gcaacggcgg ctactactgg ccgctcaacg acggcacgac gacgacggac
     4441 ttccgtatcc acccggtgct ggtggcgaac gatccggccg cgctacaccc ggcgctgctg
     4501 accggggaag gcctgatgct caccgccgac gtcatggtca aggcatacgc cgagcacggc
     4561 tacatccagc gcgtgctggc cggctggacg ggcccgtcgg ccgagatgaa cgccgtgttt
     4621 ccgcgcggcc agggcaagtc acccaaggtt cgcgcgttcg tcgacttcct gatcgagcgc
     4681 gtcaagttcg acgccgacta catggacgag ctgtgcccaa accaccagtg ctgcgaggca
     4741 gagatgcgcg tggcggaggc cacggcgacg ctcggcatcg acgccgtcgt gcaggtgccg
     4801 acgcccgtgc tggccgaggc gtgaagccgg cctgggcgcc ggccagcatg cagccggaaa
     4861 cagtgctcga gttctggttc gacgcgctcg agccggatca atggtggtcg gtcgatccgc
     4921 agttggacgc gacgatccgc gaccgcttcc tgccgctgtg gcaccgggcg atcgcgggcg
     4981 aactgtatcc gtggcgcggc accgcacccg ggcgcgtggc cgaggtgatc gtgctcgacc
     5041 agttctcgcg caacgtcttt cgcgacaccc ccgccgcgta cgcccaggac ccgatcgcgc
     5101 tcgtgctcgc ccaggaggcc gtggcgtcgg gtgcgctcga ggcc
//
LOCUS       DASPFR010000216         8138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1527040, whole genome shotgun sequence.
ACCESSION   DASPFR010000216 DASPFR010000000
VERSION     DASPFR010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8138
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527040"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VD737_05515"
     CDS             complement(<1..602)
                     /locus_tag="VD737_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10050.1"
                     /translation="MTGRSLASLVAAALLLAACGGGGAGSDSGDDGGTAGGPTNAPTK
                     TTPGVWQGTITSSMNGARSLIGMTGDDGRSVWMTTDGRVWTGQVPMSGTQVDVSMAGH
                     MYPGDHFPDGTIHGTSAMHMDYAEEHWSGHYSGAGDDGTFTMSRHAAWDRPASLAALA
                     GTYTRTTSIGYTMTMSVSASGEFMASDTRGCVINGSVTVP"
     gene            860..1276
                     /locus_tag="VD737_05520"
     CDS             860..1276
                     /locus_tag="VD737_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HXW10051.1"
                     /translation="MHAMQMISTHPDVRGSTNDELIRCIEECYDCAQTCVSCADACLA
                     EAGVAELRQCIRLNLDCADVCAATGRLASRRTGSNEHLLRAMIEACAEACRLCGSECA
                     EHASQHEHCAICAEACRRCEQACMTAAGTITPSRRQ"
     gene            complement(1319..2617)
                     /locus_tag="VD737_05525"
     CDS             complement(1319..2617)
                     /locus_tag="VD737_05525"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXW10052.1"
                     /translation="MSRPITARVAWLIGASLATFLARAESLDDAWAMALRQDAVLAAT
                     RNQAQAAELDAQAARGQRWPTIAVTGGYTRLDDSPAFDFAWTGLPIQPPPLFEDDAFV
                     TGAATVTLPLFASGQISSSIAAAEAQARGANAQLQVATGDVKLAIAEAYVDVLRARKA
                     HAVAQSSVRTLEAVASDTASLFERELVPKNELLAARVALADAKQNALRASNAAEVALA
                     AYNRRIGAPMDRAVELDERIATTTDLPGTLETLIEQALARRSELSAFDARAEAYGQMA
                     RTERARVLPQVVLTAGYQYLENQFLDDDTVGLAGIGVRWALFDGGQSRKRAAALDHTR
                     YATERLRDDARSLVQLQVRQAWLGLREARLRVDVTAGAAEQAEESLRIAQERYSAGLG
                     TQTQLLEAQTLRARAQQNRDEAALDAELSELRLARAVGAL"
     gene            complement(2614..5856)
                     /locus_tag="VD737_05530"
     CDS             complement(2614..5856)
                     /locus_tag="VD737_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXW10053.1"
                     /translation="MTERDTTGRLGLSGRVAKLFLGSQLTPLLGLTALLLGLFAVLVT
                     PREEEPQINVTMANVIVPFPGASAQQVENLVATPMEKVLAEISGVKHIYSVSRPGVAV
                     LTVQFEVGEERTSAIVRLWNAIYSNQDWRPAGVGIGQPLVKPKGIDDVPILSLTLWTR
                     DVAKGAYELRQVAHAVESELKRVPGTRNVYTIGGPDDTVHVTLDPQRLAGHGLTVAEL
                     AGALQAANVVQHAGTLVTPQGVLPLQAGEFLASREDVAQLIVAMRDGHAVYLQDVATV
                     ASGADQPEQYVTFGTGPAAGRKGIDFVGTSPAVTLAVSKKPGENAIDVTDAVLARVQN
                     LRGTYVPEGVELTVTRNYGETANDKAVKLIQKLMLATGSVVLLVWLTVGWREAAVVGA
                     AVVITLAATLFASWAYGFTINRVSLFALIFAIGILVDDAIVVVENIHRHLALHPGASL
                     GELIPRAVDEVGGPTILATFTVIAALLPMAFVSGLMGPYMSPIPINASMGMLISLAIA
                     LAFTPWLSQKLLAHHHAGGHEQAQDGRLLRFFDRLMSPFLRGEQARPHRHKLYLGTVV
                     AIALAVSLAAVQWVVMKMLPFDNKSEFQVVLDMPEGTPVEETARVLDELAAEVARRPE
                     VTDYQAYAGTSAPINFNGLVRQYYLRSGPEVGDLQVNLVDKHHRDKQSHEIALKVRPA
                     LAAIGRKYGGSVKVVEVPPGPPVQAPLVAEVYAPFQATRQALAREVRQVFDQTDGVVD
                     VDDSLEAPNPRLVATVDRARAARLGVDQAETAQALAAALGGLDATYVHTGSETYPVPV
                     RLELGVPDKADLASVLSLRVRSRTGALVPLSEVTTVTRHDWDEAIYHKDLLPLAWVTA
                     DSVGRTDSPLYGMFAMVGALRDELPSGTVVEQFFFDQPDDPYRPSVKWDGEWQITYET
                     FRDMGLAYSVGLVLIYLLVVAQFGSYAVPLVIMAPIPLTIIGVMPGHALLGAQFTATS
                     MIGMIALAGIIVRNSILLVDFINLELAAGRPLADAVIRAAAVRARPIALTAVAAMTGA
                     FFILDDPIFNGLAVSLIFGILVSTALTLVLIPILYFSYRERQAAKRLVQP"
     gene            complement(5849..6931)
                     /locus_tag="VD737_05535"
     CDS             complement(5849..6931)
                     /locus_tag="VD737_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXW10054.1"
                     /translation="MHPRAVTRIASQAAAVLLLLSGCDAGAPVTQPPVSPSGLATVEV
                     GTSEAPAGLGLDGVVEAVDRATLSAQTSGRVSEVPVDVDDAVKQGQVLLRLSATEQVS
                     VLREAQAALAAATSREAQARAQHDRIRDMYERKVVARATYYEALAMRDGARAALAAAT
                     ARVAAAREGVGYTEVRAPFDGVVTDKRVQPGETVTPGTPLLSVASLAATRVIADVPQS
                     VAPALRTDPRATVRAGDVEFEASSVTLYPSAQPQSGTVRARIDLPAGAPTLAPGTFAK
                     VVIHGGDDRRLLVPRSAVVERSELRAVYVVRADQRVSFRQVRLGRPVGDRVEVIAGLA
                     RGERIATDPSAAALAARAAATNSEHD"
     gene            complement(6946..7146)
                     /locus_tag="VD737_05540"
     CDS             complement(6946..7146)
                     /locus_tag="VD737_05540"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HXW10055.1"
                     /translation="MNVDRLVFRFAGLMVLLSLALGIFVSANWFWLTAFVGANLLQAS
                     FTGFCPLAILLKKSGVQPGSAF"
     gene            7224..7595
                     /locus_tag="VD737_05545"
     CDS             7224..7595
                     /locus_tag="VD737_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXW10056.1"
                     /translation="MRNRQGQKPVGAGAVMPDLRRIAEMHDNAGAVAAFLKALGNEQR
                     LLILCNLLERPLAVGELNERVELSQSALSQHLAVLRDAGFVECRREAQLIYYSVKPGP
                     VTRVMAVLQEVFCPPARGSPS"
     gene            complement(7516..>8138)
                     /locus_tag="VD737_05550"
     CDS             complement(7516..>8138)
                     /locus_tag="VD737_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HXW10057.1"
                     /translation="LWLNLVTNGIQDVSLAFEGRRGDELRAPPRSPGEPLFDRLMLER
                     VLLGGTWMAVLGFAVYAWLLESGVAVWQARNTLMLLMVLLQNVDTFNARSETVSAFRM
                     PLARNPLLVAGVTTALLLHVGALHVPPLQHLLEFGPVAAVTWLWLGPAALSLLLVMEL
                     HKTSWRRRTGSRTQPREYAGPTMASPGPADRTLPAAPPSRGSPGQA"
BASE COUNT         1250 a         2863 c         2823 g         1202 t
ORIGIN      
        1 tccggaacgg tgacgctgcc gttgatcacg cagccgcgcg tgtcgctggc catgaactcg
       61 cctgacgcgg agacggacat cgtcatcgtg tagccgatcg aagtcgtgcg ggtgtaggtg
      121 ccggcaagcg cggccagcga cgccggccgg tcccaagccg cgtgccggga catcgtgaag
      181 gtcccgtcgt cgcctgcgcc gctgtagtgg ccgctccagt gctcctcggc gtagtccatg
      241 tgcatcgccg acgtgccgtg gatcgtaccg tccgggaagt ggtcaccggg atacatgtgc
      301 ccggccatcg aaacgtcgac ctgcgtaccc gacatcggaa cctgtccggt ccagacgcgc
      361 ccgtccgtgg tcatccacac gctgcggccg tcgtcgccag tcatgccgat caggctgcgg
      421 gcgccgttca tggacgacgt gatcgtgcct tgccagacgc ccggtgtcgt cttcgtcgga
      481 gcgttcgtgg gtccaccggc cgtgccgccg tcatccccgg agtccgaccc ggcaccgccg
      541 ccaccgcacg cggcgagcag caaggcggcc gcgacgagcg aagccagtga tctgccagtc
      601 atgggagtct ccgcacgaag gccggcgcca tcgccgaagt gccgatttgg gaccggcggg
      661 cgcgcccgtt caacgaggag taatgcctag ccgcactcgg gcccacgaag cgatacgcgc
      721 ggcaatggcg gactgcgacg cagcgagcag cacgcagcgg cgtcgtccca cggctcgtgt
      781 tgccggcctc cggtaacact cccgcgtgcc ggcgactacg gtgcgccact cgtcatcgcc
      841 aagcaccagg aggcccccga tgcacgccat gcagatgatc agcacgcacc cggacgtccg
      901 cggcagcacc aacgacgaac tgatccggtg catcgaggag tgctacgact gtgcgcagac
      961 ctgcgtctcg tgcgccgacg cgtgcctcgc cgaagcgggg gtcgcggagt tgcgacagtg
     1021 catccggctg aacctcgact gcgccgacgt gtgtgcggcg accgggcgcc tcgcttcacg
     1081 ccgcacgggc agcaacgagc acctgctgcg cgccatgatc gaggcctgcg ccgaggcttg
     1141 ccgcctgtgt ggctcggaat gcgcggagca cgcctcgcag cacgagcact gcgccatctg
     1201 cgcggaagcc tgtcgtcgtt gcgagcaggc gtgcatgacg gcggccggga cgatcacgcc
     1261 gtcacgtcgg cagtaggggg cactccggcg cggccggccg cgaccgcttt gccgcgagtc
     1321 agagcgcccc gaccgcccgc gcgagccgga gctcggacaa ctccgcgtcg agtgcggctt
     1381 cgtcgcggtt ctgctgcgcc ctggcccgca gggtctgcgc ctccagcagc tgagtctgcg
     1441 ttcccagtcc tgccgagtag cgttcctgcg cgatgcgcag gctctcctcc gcctgctcgg
     1501 cggcgccggc cgtgacgtcg acgcggagac gcgcttcgcg cagccccagc cacgcctgtc
     1561 ggacctgcaa ctgcacgagt gaccgcgcgt cgtcgcggag ccgctcggtg gcgtagcggg
     1621 tgtggtcgag cgcggcggcg cgcttgcgcg actgtccgcc gtcgaacagt gcccagcgca
     1681 ccccgatccc ggcgagcccg acggtgtcgt cgtcgaggaa ctggttttcg aggtactggt
     1741 agcccgccgt caacacgacc tggggcagca cgcgtgcgcg ttcggtgcgg gccatctggc
     1801 cgtacgcttc tgcccgcgcg tcgaaggcgg acagttccga acgccgcgcc agggcctgtt
     1861 cgatcagcgt ctcgagggtg cccgggaggt ccgtggtcgt cgcgatgcgt tcgtccagtt
     1921 ccaccgcccg atccatcggc gctccgatgc gacggttgta cgccgcgagc gcgacctccg
     1981 cggcgttgga cgcccgcagg gcgttctgct tcgcgtcggc gagcgcgacc cgtgcggcga
     2041 gcagctcgtt cttcggcacg agctcgcgtt cgaagaggct ggcggtgtcg ctcgcgaccg
     2101 cttcgagcgt gcgcacgctg ctctgcgcca ccgcgtgcgc cttccgcgcg cgcaagacgt
     2161 ccacgtaagc ctccgcgatc gccagcttga cgtcgccggt cgcgacctgg agttgcgcat
     2221 tcgcgccgcg cgcctgcgcc tcggctgccg cgatgctcga cgagatctgg ccgctggcaa
     2281 aaagcggcaa cgtcacggtc gcggcaccgg tcacgaaggc atcgtcctcg aagagcggcg
     2341 gcggctggat cggcagcccg gtccatgcga agtcgaacgc gggcgaatcg tcgagcctgg
     2401 tatagccccc ggtgaccgcg atcgtcggcc accgctggcc ccgggcggcc tgggcgtcca
     2461 gctcggccgc ctgcgcctgg ttgcgggtcg cggcgagcac ggcgtcctgc cgcagggcca
     2521 tggcccacgc atcgtcgagt gactcggcac gcgccaggaa cgtcgcgagc gacgcgccga
     2581 tcagccatgc gacacgggcg gtgattgggc ggctcatggc tggaccaacc tcttcgcagc
     2641 ctggcgctcg cggtagctga aatacaggat cgggatcagg accagcgtga gcgcggtcga
     2701 gaccaggatc ccgaagatca gcgatacggc gaggccgttg aagatcggat cgtcgaggat
     2761 gaagaacgcg ccggtcatgg cggcaacggc cgtcagcgcg atcggtcgcg cgcgtaccgc
     2821 ggcggcgcgg atcacggcgt cggccaacgg acgcccggcg gcaagctcga ggttgatgaa
     2881 gtccacgagc aggatcgagt tgcgcacgat gatgccggcg agcgcgatca tgccgatcat
     2941 cgaggtcgcc gtgaattgcg cgccgagcaa cgcgtggccg ggcatcacgc cgatgatcgt
     3001 cagcgggatc ggcgccatga tcacgagcgg cacggcgtac gaaccgaact gcgcgaccac
     3061 cagcaggtag atcagcacga ggcccaccga gtacgcgagc cccatgtcgc ggaacgtctc
     3121 gtacgtgatc tgccactcgc cgtcccactt gacgctgggc cggtacggat cgtcgggctg
     3181 gtcgaagaag aactgctcga cgacggtgcc cgagggcagc tcgtcccgca gcgcgccgac
     3241 catcgcgaac atgccgtaca gagggctgtc ggtgcgcccg accgaatccg ccgtgaccca
     3301 cgcgagcggc agcaggtcct tgtggtagat cgcctcgtcc cagtcgtgac gcgtgacggt
     3361 cgtcacctcc gacaacggga cgagcgcgcc cgtgcgcgaa cgcacccgca gcgagagcac
     3421 gctcgcgagg tccgccttgt ccgggacccc gagttccagc cggaccggca cgggataggt
     3481 ctcgctgccg gtgtgcacgt aggtggcatc gaggccgccc agcgccgcgg ccagcgcctg
     3541 ggccgtctcg gcctggtcga cgcccaggcg cgcggcgcgg gcgcggtcca ccgtcgccac
     3601 gaggcgcggg ttgggcgctt cgaggctgtc gtcgacgtcg accacgccgt cggtctggtc
     3661 gaacacctgg cgcacttccc tcgcgagcgc ctgccgcgtc gcctggaacg gtgcgtacac
     3721 ctccgccacc agcggcgcct ggaccggcgg ccccggcggc acctcgacga ccttgaccga
     3781 gccgccgtac ttgcggccga tcgcggcgag cgccgggcgg acttttagcg cgatctcgtg
     3841 actctgcttg tcgcggtgat gcttgtcgac gaggttgacc tgcaggtcgc ccacttcggg
     3901 tccactgcgc aggtagtact gccgcaccag cccgttgaag ttgatcgggg ccgacgtgcc
     3961 cgcgtaggcc tggtagtcgg tcacctcggg acggcgggcg acctcggccg ccagttcgtc
     4021 gagcacgcgt gcagtctcct cgaccggcgt tccctccggc atgtccagca cgacctggaa
     4081 ctccgacttg ttgtcgaagg gcagcatctt catgaccacc cactgcaccg ctgccagcga
     4141 gacggcgagc gcgatcgcca cgaccgtgcc caggtagagc ttgtgccggt gcggtcgtgc
     4201 ctgctcgccg cgcaggaacg gcgacatcag ccggtcgaag aagcgcagca gcctgccgtc
     4261 ctgggcctgc tcgtggccgc cggcgtggtg gtgggcgagc agcttctgcg acagccaggg
     4321 cgtgaacgcg agcgcgatcg ccagcgaaat cagcatgccc atgctcgcgt tgatcggaat
     4381 ggggctcatg tacgggccca tcaggccgct cacgaacgcc atgggcagca gcgccgcgat
     4441 gaccgtgaac gtcgcgagga tcgtcggccc gcccacctcg tccacggcgc gcggtatcag
     4501 ttcccccagc gaagctcccg ggtgcagcgc caggtggcgg tggatgttct cgacgaccac
     4561 gatcgcgtcg tcgaccagga tgccgatcgc gaagatcagc gcgaacagcg agacccggtt
     4621 gatcgtgaag ccgtaggccc aggaggcgaa cagcgtcgcg gccagcgtga tcacgacggc
     4681 ggcgcccacg acggccgcct cgcgccaacc caccgtgagc cagaccagca gcaccacgga
     4741 acccgtcgcg agcatcagct tctggatcag cttgacggcc ttgtcgttcg cggtctcgcc
     4801 gtagttgcgc gtgacggtca gctccacacc ctcgggcacg tacgtgccgc gcaggttctg
     4861 tacgcgggcc agcacggcgt cggtcacgtc gatcgcgttc tcgccgggct tcttggaaac
     4921 cgcgagcgtg accgcgggcg aggtcccgac gaagtcgatg cccttgcgtc ccgcggccgg
     4981 gccggtgccg aacgtgacgt actgctcggg ctggtccgcg ccggacgcca cggtggcgac
     5041 atcctgcagg taaacggcgt gcccgtcgcg catcgcgacg atcagctgcg cgacgtcttc
     5101 gcgcgaggcg aggaactcgc cggcctgcag cggcagcacg ccctgcggcg tgaccagcgt
     5161 cccggcgtgc tgcacgacgt tggccgcctg cagcgcgccc gcgagttccg ccacggtcag
     5221 tccgtgaccg gcgagccgct gcgggtcgag cgtcacatgg accgtgtcgt cgggtccccc
     5281 gatcgtgtag acgttgcgcg tgcccggtac gcgcttgagt tcggactcga cggcgtgcgc
     5341 cacctgcctg agctcgtacg cgcccttcgc gacgtcgcgt gtccagagcg tcaggctcag
     5401 gatcggaacg tcgtcgatcc ccttcggctt cacgagcggc tgcccgatcc cgaccccggc
     5461 cggccgccag tcctggttcg agtagatcgc gttccagagt cgcacgatcg cggaggtgcg
     5521 ctcctcgccg acctcgaact gcacggtcaa cacggcgacg ccgggcctgg acacggaata
     5581 gatgtgcttc acgccggaga tctcggcgag cactttctcc atcggcgtgg ccaccaggtt
     5641 ctcgacctgc tgcgcgctcg cgcccgggaa cggcacgatc acgttggcca tggtcacgtt
     5701 gatctgtggc tcttcctcgc gcggcgtcac gagtaccgcg aacaggccga gcagcagcgc
     5761 cgtgaggccg agcagcggcg tcagctggct gccgaggaac agcttcgcga cgcggccgct
     5821 gaggccgagc cgccccgtcg tgtcccgctc agtcatgttc ggagttcgtc gccgcggccc
     5881 gggcggccag ggcggcggcc gagggatccg tggcgatgcg ttctccgcgc gccaggcccg
     5941 cgatcacttc gacccggtca ccgaccggcc gaccgagacg cacctggcgg aacgagaccc
     6001 gctgatccgc tcgcaccacg tacacggcgc gcagttcact gcgctcgacg acggcgctgc
     6061 gggggacgag cagtcggcgg tcgtcgccgc cgtggatgac caccttggcg aaggttccgg
     6121 gcgcgagcgt aggggccccc gccggcaggt cgatgcgagc ccgaacggtg ccggactgcg
     6181 gttgcgcgga cgggtacaac gtaaccgacg aggcctcgaa ctcgacgtcg ccggcccgaa
     6241 cggtcgcgcg cggatcggta cgcagcgccg gcgcgacgct ctgcgggacg tccgcgatga
     6301 cgcgcgtcgc cgcaagtgac gcgacggaca ggagcggcgt gccgggcgtg acggtttcgc
     6361 ccggctgcac gcgcttgtcg gtgacgacgc catcgaacgg tgcccgcacc tcggtgtaac
     6421 ccacgccttc ccgggccgcc gcgacccgcg ccgtcgcggc cgccagcgcg gcccgcgcgc
     6481 cgtcgcgcat ggcaagcgcc tcgtagtagg tggcacgcgc gacgaccttg cgctcgtaca
     6541 tgtcacggat gcggtcgtgc tgggcacgag cctgcgcctc acgggaagtg gcagccgcga
     6601 gtgccgcctg cgcctcacgc agcaccgaca cctgctcggt cgcgctcagg cgcagcagga
     6661 cctggccctg cttcaccgcg tcgtcgacgt cgaccggcac ttcggacacg cgccccgacg
     6721 tctgggccga cagcgtcgca cgatcgaccg cctcgaccac gccgtcgagc cccaggcccg
     6781 ccggcgcctc ggacgtcccc acctcgaccg tggcgaggcc ggacgggctc acgggaggtt
     6841 gcgtcacggg tgcaccggcg tcgcagccgg acagcagcag cagtacggca gccgcctgcg
     6901 acgcgatgcg cgtgacggcg cgtgggtgca tgacgtcccg gccgttcaga acgcggaacc
     6961 cggctgcacg ccggacttct tcagcaggat cgcgagcgga cagaagcccg tgaacgatgc
     7021 ctgcagcagg ttcgcgccga cgaaggccgt cagccagaac cagttcgcgc tgacgaagat
     7081 gccgagcgcc aggctcagca ggaccatgag gcccgcaaac ctgaatacga gacggtcgac
     7141 gttcatgtgt tgctccttcg gaaacgtacg gtcgcattta tattagctat ttctaattta
     7201 gtcaattccg atatactcac gccatgcgca accggcaagg ccagaagccc gtcggcgcgg
     7261 gggcagtgat gcccgacctg cgtcggatcg cagagatgca cgacaatgca ggtgccgtcg
     7321 ccgcattcct gaaggcgctc ggcaacgagc agcggctgct gatcctctgc aacctgctcg
     7381 agcgcccgct cgcagtgggc gaactcaacg aacgcgtcga gctcagccag tcggcgctgt
     7441 cgcagcacct ggctgtactg cgggatgcgg gattcgtgga gtgccgtcgc gaggctcaat
     7501 tgatctatta ctcggtcaag cctggcccgg tgacccgcgt gatggcggtg ctgcaggaag
     7561 tgttctgtcc gccggcccgg ggctcgccat cgtaggtccc gcgtactcgc gcggctgcgt
     7621 ccggctgccc gtgcggcggc gccagctggt cttgtgcaac tccatcacca gcagcaggct
     7681 gagggcggca gggcccagcc agagccaggt cactgcggcc accggaccga actcgagaag
     7741 gtgctgcagg ggcggcacgt gcagcgcacc gacgtgcagc agcagcgcgg tcgtgacacc
     7801 agccaccagc agcggattcc gtgcgagggg catgcggaat gccgagacgg tctcggaccg
     7861 ggcgttgaac gtgtcgacgt tctgcagcag caccatcagc agcatcagcg tgttccgcgc
     7921 ctgccacacc gcgacccctg actcgagcag ccacgcgtag accgcgaagc cgaggaccgc
     7981 catccaggtg cccccgagca ggacccgctc cagcatgagc cggtcgaaca gcggctcgcc
     8041 cggactgcgt ggcggcgcgc gcagctcgtc gccgcgccgg ccttcgaagg ccagcgagac
     8101 atcctggatg ccgttggtga cgaggttgag ccagagca
//
LOCUS       DASPFR010000217         5714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_915560, whole genome shotgun sequence.
ACCESSION   DASPFR010000217 DASPFR010000000
VERSION     DASPFR010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915560"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>5714
                     /locus_tag="VD737_05555"
     CDS             <1..>5714
                     /locus_tag="VD737_05555"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10058.1"
                     /translation="DCSDYPGGVLDGFAGTIAPSQLQIDRICTIRNYPASNPLTTNFS
                     FLTQPGQTDERWLIIFDNVVHTGQMACNAVAGHRIWFVNGSSTSIKDGCQNLLIPVEK
                     IDKKNPGDNTQTTATIGVPFTYKLTMPVLFAPATSVVIDWAGSPNDLHSVVLTDDLNA
                     TGVDLTYLSHVAYIQGTNTPVPHTFTNVGGLLTFQDFGVIPAGQQIIIEITVVLDDSP
                     ANVLGTQFINTAKWSFGRLIEGTFYEPLPGEWGISPPLTIAAPQLVVTKTGPATMNLG
                     QWATFALDVRNAGQFEAWNATLLDRLPDVAAGGMCAQTPEVLDAQVFAADGVTPVAGK
                     GPLASGTDYSLAYVGAPTCELRFTALTPKATIGASQRLVVRYRTRLDADTQNGATLTN
                     VAGALEWFNGPATATERVAYQRTLTNGTPGTADHEDDHTVTVAFVGDFFDKTVANLTR
                     GTNPATTAQPGDRLRYTLRLRSSGGIASFRLRDELDALNAGASFVPGSLSLVGALPAG
                     AVNNSSANGGARGTGLIDIGNLSLAAGGELLLQFDVTLASALLEDTVVSNQSIVLVGG
                     ATYALSDDPNVNGIADPQVAGDEDPTRVTITLPLPTALAKANTQATASVGEVFRYRIT
                     VPSAPFAFPLYDVRILDDLGASAADLRFVSVTKVSGSQPWTPVNLGTATNLVIADPTV
                     GIDIPAGEQVVVEVAVLLENTATNVAGLQFTNTASYTFNRANGNVTTQRPGGPGTTAP
                     MTIVAPDQLTLEKSGPTDMGIGVPATFTLDVHNPSAGAAWGLTITDRLPSVATGGMCD
                     AAPTQVTAQLFQADGVTAAGPPLVAGTDFTAAFVGEPACTFTLKMLTPAAAVGAGQRL
                     IVRYEALLDTDTENGTSLTNVAGATEWFSANPAEPAAAGQLVTYTRTLTDGTLNVLDH
                     EDAHTTLVLRPTMRFEKTVANVTTGANPAVEARPGDTLRYTLRVENLSDVALAGFALR
                     DELDRLNATAAFAAGSLTVVSAPAGADLSNIDASGGAKGSGLLDVRNLTLPASGGTVT
                     VVFDVRLAPVIANGTLVANQSYLNVNAATLALSDDPNVNGQANPDVPNDDDPTRVRIV
                     SAPSFVVQKISTDLTGDANVLLAGETLRYTLTVRNVGTDNATAVVLRDAVPVNTTYVP
                     GSTTLNGAAVADAGGLSPLVNGIAIHAPIDATPGSLPAGPADSAAHLATITFDVVVDP
                     SAVDGTVISNQGFVSSPPAGVVDRPSDDPTTPIVDDPTRDIVGSLPLLYADKRVALQV
                     DLGSPGIVDPSDVLRYTITVTNTGAVPATGVVVTDPVPANTTYVTDTTLLNGAPYGQP
                     DAGAAPLAGGIAVGTLAPGASATVQFDLRVNPGTPSGTVISNQAVVYSAEIPNLLTDG
                     DGNPATGPEPTVVVVGAGQQLTIAKEVAVVGGGPAVAGATLEYTVRVTNVASVPAQYV
                     RITDDLPAQYLTYVDGSASLNGSSSGTSFAGTLLTADYGATHGALEPGGVVVLRFRAV
                     LDANLPIGTVVTNTAVAYWNDPPQTASASVSIAVGGVPGVGVLNGSAWHDANFDDVQD
                     AAERPLTGWTVDLYRDDQAVASVQVDASGVYRLSGLAPNYATSVRYELRFRAPGAGAR
                     TALLGLADSPYTNGLQRISDIVVESGANLQGLNLPIDPNGVVYNSVARTPIAGATLTL
                     LNSGSGTALPSACFDDAAQQGQVTLGEGWYKFDLNFSDPACPSGGDYLIGVTAPGTAY
                     VGGYSQIIPPTSGPATAPLSVPSCPLSADDAVPGTATYCEAQPSEFAPGVAIAARSAG
                     TRYHARLGLDASQRPGSSQIFNNHIPLDPDLSGAVAISKTTPLMNVTRGQLVPYTITV
                     NNVAGLVLTDVAIVDRYPAGFTYVEGSARLNGTPTEPSVAGREMRWSGLSIAGSAQYT
                     VQIL"
BASE COUNT          909 a         2104 c         1907 g          794 t
ORIGIN      
        1 acgattgctc cgactacccg ggtggcgtgc tcgatggctt cgcgggaacg atcgctcctt
       61 cgcagctgca gatcgatcgg atctgcacga tccgaaacta tcccgcatcg aatccgctga
      121 ccacgaactt cagcttcctc acgcagccgg gtcagaccga cgagcggtgg ctgatcatct
      181 tcgacaacgt ggtgcacacc ggccagatgg cctgcaacgc ggtggcgggc caccgcatct
      241 ggttcgtcaa cggttcctcg acctcgatca aggacggctg ccagaacctg ctgatcccgg
      301 tcgagaagat cgacaagaag aaccccgggg acaacacgca gacgacggcc acgatcggcg
      361 tgccgttcac gtacaagctg acgatgccgg tgctgttcgc gccggccacc agcgtcgtca
      421 tcgactgggc cgggtcgccc aacgacctgc acagcgtcgt gctgaccgac gacctgaacg
      481 ccacgggcgt ggacctcacc tacctgagtc acgtggccta catccagggc acgaacacgc
      541 cggtgccgca cacgttcacg aacgtcggcg gcctgctgac gttccaggat ttcggcgtca
      601 tcccggccgg gcagcagatc atcatcgaga tcacggtggt gctcgacgac tcgccggcca
      661 acgtgctcgg cacgcagttc atcaacacgg cgaagtggtc gttcgggcga ttgatcgagg
      721 gcaccttcta cgaaccgctg ccgggcgaat ggggcatctc gccgccgctc acgatcgcgg
      781 caccgcagct ggtcgtcacc aagacgggtc ccgcgaccat gaacctcggg caatgggcca
      841 cgttcgcgct cgatgtgcgc aacgccggcc agttcgaggc gtggaacgcg acgctgctgg
      901 accggttgcc cgacgtcgcg gcggggggca tgtgcgcgca aacccccgag gtgctcgacg
      961 cacaggtatt cgcggcggac ggtgtgacgc cggtggcggg caagggcccg ctcgcctcgg
     1021 ggacggatta ctcgctggcc tacgttggcg caccgacctg cgaactgcgg ttcaccgccc
     1081 tcacgcccaa ggcgacgatc ggcgccagtc agcgcctcgt cgtgcgttac cgcacgcgtc
     1141 tggacgccga cacgcagaac ggcgcgacgc tgaccaacgt ggcgggcgcg ctggagtggt
     1201 tcaatggccc ggcgaccgcg accgagcgcg tcgcctacca gcgcacgctg accaacggca
     1261 cgccaggcac cgccgaccac gaagacgacc acacggtcac cgtggcgttc gtcggcgact
     1321 tcttcgacaa gacggtcgcg aacctgacgc gcgggaccaa cccggccacc accgcccagc
     1381 cgggtgaccg gctgcggtac acgctgcggc tgcgcagcag cggcggcatc gcgtccttca
     1441 ggctgcgcga cgagctcgac gcgctcaatg ccggcgcgtc gttcgtgccc gggtcgctga
     1501 gcctggtcgg agcgctgccg gcgggcgccg tcaacaacag cagtgcgaac ggtggcgcgc
     1561 ggggcaccgg cctgatcgac atcggcaacc tgagcctggc ggccggcggc gagctgctgc
     1621 tgcagttcga cgtgacgctg gcctcggcac tgctcgagga caccgtcgtc tccaaccagt
     1681 ccatcgtgct ggtcggcggt gcgacgtacg cgctcagcga cgacccgaac gtcaacggca
     1741 tcgcggaccc gcaggtggca ggagacgagg atccaacccg cgtcacgatc acgctgccgc
     1801 tgcccacggc actcgcgaag gcgaacacgc aggcgaccgc gtcggtcggc gaggtgttcc
     1861 gttatcgcat cacggtgccc tcggcgcctt tcgcgttccc gctgtatgac gtgcgcatcc
     1921 tcgacgacct cggcgcctcg gccgccgacc tgcgcttcgt cagcgtgacc aaggtgtcgg
     1981 gctcgcagcc gtggactccg gtcaacctgg gcaccgcgac caacctcgtg atcgctgacc
     2041 cgacggtggg catcgacatc ccggccggcg agcaggtcgt ggtcgaggtg gccgtgctgc
     2101 tcgagaacac ggcgaccaac gtggccggcc tgcagttcac gaacaccgcc tcgtacacgt
     2161 tcaaccgcgc gaacggcaac gtgacgacgc aacggccggg cggtccgggc acgaccgcgc
     2221 cgatgacgat cgtggcgccc gaccagctca cgctcgagaa gagcgggccg accgacatgg
     2281 gcatcggcgt gccggcgact ttcacgctcg acgtccacaa ccccagtgcc ggtgcggcct
     2341 gggggctcac gatcaccgac cggctgccga gcgtcgccac cggtggtatg tgcgacgcgg
     2401 cgccgaccca ggtcaccgca cagctgttcc aggcggacgg cgtcacggcc gccggtccgc
     2461 cactcgtggc ggggacggat ttcaccgccg cattcgtcgg cgaacccgcg tgcacgttca
     2521 cgctgaagat gctgacgccc gccgccgccg tcggggctgg ccagcgcctg atcgtccgct
     2581 acgaggcgct gctggacacg gacaccgaga acggcacgtc gctgaccaac gtcgcgggtg
     2641 cgacggaatg gttcagcgcg aaccccgccg agccggccgc cgccggccag ctcgtcacgt
     2701 acacccgcac gctcaccgac ggcaccctga acgtgctcga tcacgaggac gcgcacacga
     2761 cgctggtact gcgtcccacg atgcggttcg agaagaccgt cgccaacgtg acgacgggcg
     2821 cgaaccccgc cgtcgaggcg cgaccggggg acaccctgcg ctacacgctg cgcgtcgaga
     2881 acctgtccga cgtcgcgctc gccggcttcg cgctgcgaga cgagctcgac cgcctgaacg
     2941 cgacggcggc gtttgccgcc ggatcgttga cggtggtgtc ggcgccggcc ggcgcggacc
     3001 tcagcaatat cgacgcctcc ggcggcgcga agggcagcgg cctgctcgac gttcgcaacc
     3061 tgacgttgcc ggcctcgggt ggcaccgtca cggtggtctt cgacgtgcgg ctcgccccgg
     3121 tgatcgccaa cggcacgctg gtggcgaacc agtcctacct gaacgtcaac gccgccacgc
     3181 tcgcgctcag cgacgacccg aacgtcaacg gccaggcgaa ccccgacgtg ccgaacgacg
     3241 atgacccgac gcgcgtgcgc atcgtgtcgg cgccctcgtt cgtcgtgcag aagatctcca
     3301 ccgacctgac cggcgacgcg aacgtgctgc tcgccggcga gacgctgcgc tacacgctca
     3361 cggtccgcaa cgtcggcacc gacaatgcga ccgcggtcgt gctgcgcgac gccgtgccgg
     3421 tcaacacgac ttacgtgccc ggcagcacca cgctgaacgg cgccgcggtc gccgacgccg
     3481 gcggactgtc cccgctggtc aacggcatcg cgatccacgc gccgatcgac gccaccccgg
     3541 gctcgctgcc cgcgggcccg gccgacagcg ccgcgcacct ggcgacgatc acgttcgacg
     3601 tcgtggtgga cccgtcggcg gtcgacggca cggtcatctc gaaccagggc ttcgtcagct
     3661 cgccgccggc cggcgtcgtc gaccgaccct cggacgaccc gaccacgccg atcgtcgacg
     3721 acccgacgcg cgacatcgtg ggcagcctgc ccctgctgta cgcggacaag cgcgtggcac
     3781 tgcaggtcga cctgggctcg ccaggcatcg tcgaccccag cgacgtgttg cgctacacga
     3841 tcaccgtcac caacacgggt gccgtcccgg cgaccggcgt ggtcgtgacc gacccggtcc
     3901 ccgcgaacac cacctacgtc acggacacga cgctgctgaa cggcgcgccc tacgggcagc
     3961 ccgatgccgg tgcggcaccg ctcgccggcg gcatcgccgt gggcacgctc gcgccgggcg
     4021 cgagcgcgac cgtgcagttc gacctgcgcg tgaacccggg cacgccgtcc ggcaccgtga
     4081 tcagcaacca ggcggtcgtc tacagcgcgg agatccccaa cctgctcacc gacggtgacg
     4141 gcaacccggc cacgggcccc gaacccacgg tcgtcgtcgt gggcgccggc cagcagctca
     4201 cgatcgcgaa ggaagtcgcg gtcgtcggtg gcggtccggc ggtcgccggc gcgacgctcg
     4261 agtacacggt gcgcgtcacc aacgtcgcgt cggtgccggc gcagtacgtc cggatcaccg
     4321 acgacctgcc ggcgcagtac ctcacctacg tggacggctc tgcgagcctg aacggctcgt
     4381 cgagcggcac gagcttcgcg ggcacgttgc tcacggccga ctacggcgcg acccatggcg
     4441 cgctcgagcc gggcggcgtc gtggtgctgc gcttccgcgc cgtgctcgac gcgaacctgc
     4501 ccatcggcac ggtcgtgacg aacacggccg tcgcctattg gaacgacccg ccgcagacgg
     4561 cgagtgccag tgtctcgatc gccgtcggtg gcgtgccggg ggtgggcgtc ctgaacggtt
     4621 ccgcgtggca cgacgccaac ttcgacgacg tgcaggacgc cgcggagcgc ccgctcaccg
     4681 gctggaccgt cgacctctat cgggacgatc aggcggtggc ctcggtgcag gtcgacgcgt
     4741 cgggcgtcta tcgcctgagc ggcctcgcgc cgaactatgc gaccagcgtc cgttatgagc
     4801 tgcggttccg cgcgccgggc gccggtgcgc gcacggcgct gctcggcctc gcggattcgc
     4861 cgtacacgaa cggtctgcag cgcatctccg acatcgtcgt cgagtccggc gcgaacctgc
     4921 aggggctgaa cctgccgatc gatccgaacg gcgtcgtgta caactccgtc gcgcgcacgc
     4981 ccatcgccgg cgccacgctg acgctgctga actccggcag cggtacggca ctgccgtcgg
     5041 cctgcttcga cgacgcggcc cagcaggggc aggtgacgct cggcgagggc tggtacaagt
     5101 tcgacctgaa cttcagcgat ccggcctgtc ccagcggcgg cgactacctg atcggcgtga
     5161 ccgcgccggg cacggcctat gtgggcggct actcacagat catcccgccg acctccgggc
     5221 cggccacggc gccgctctcc gtgccgtcct gcccgctgag cgccgacgac gccgtgccgg
     5281 gcaccgcgac gtactgcgag gcgcagccgt ccgagttcgc gccgggcgtg gccatcgccg
     5341 cccgcagcgc gggcactcgc taccacgccc ggctgggtct cgacgccagc cagcggcccg
     5401 gctcgagcca gatcttcaac aaccacatcc cgctcgaccc ggacctgagc ggtgcggtgg
     5461 cgatcagcaa gaccacgccg ctcatgaacg tcacgcgcgg ccagctcgtg ccgtacacga
     5521 tcaccgtgaa caacgtcgcc gggctcgtgc tgaccgacgt ggccatcgtc gaccgttacc
     5581 cggccggctt cacctacgtc gaaggctcgg cgcgcctgaa cggcacgccg accgagccct
     5641 cggtcgcggg tcgtgaaatg cgctggagcg ggctctcgat cgcaggttcc gctcagtaca
     5701 ccgtgcagat cctg
//
LOCUS       DASPFR010000218         5462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1403964, whole genome shotgun sequence.
ACCESSION   DASPFR010000218 DASPFR010000000
VERSION     DASPFR010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403964"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VD737_05560"
     CDS             <1..848
                     /locus_tag="VD737_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HXW10059.1"
                     /translation="NTKLFFAETIGNPKINVLDIRTVADIAHESGVPLIVDNTIATPY
                     LIKPFEHGADIVVHALTKYLGGHGNSIGGAIVDSGKFPWAKHKARFRRLNEPDVSYHG
                     VVYTEALGEAAYIGRARVVPLRNMGAALSPFNAFLILQGIETLALRMDRICENSLVIA
                     RFLQKHPKVRWVNYAGLPDHPSHGLAEKYMGGRASGILTFGIDGGREGGARFQDALQL
                     FVRLVNIGDAKSLACHPASTTHRQLNAAELERAGVSEDMVRLSVGIEHVDDLIADLEQ
                     ALAAA"
     gene            992..2821
                     /locus_tag="VD737_05565"
     CDS             992..2821
                     /locus_tag="VD737_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HXW10060.1"
                     /translation="MKTPSSDFALARELSPRRNTTDADDVPALVTEQLRHFSIDPQTD
                     FGRRLGELAGHLYAGNVAAHQLWEVAMRELAALDQRDRVARFNAKRFLCFQLAKILDT
                     LQNPLRKTYQSLLHDPSQAAVKGPYPIFDNVTALFSATPVIARTATYLYACTEWVEDA
                     FKGREPLLEIYSRLLNPTSISLANHVVDLEAGSLAGEYLAWNFNSGMAAIDTTLANLV
                     GAQDVVLASRNVYGGTYQLLHDWYGKKSNLDVAVEWFDGFTVEDFRRALDGVRVKHRT
                     RLERGRNVYVFIESPCNPHGYVLDVPAICRAAHERGHTVIVDATVGTPVLQPVLRRKD
                     PLERPDFVIHSYTKDLAGAGTTTAGVCIGRNERMFIPKGDTVTAPGPDGKPRTYVWDE
                     SLFWNVYYVKGAFLDADKAFEVINGMRTVELRVLQKAINTIVLARALARHPAFGVRCG
                     AVEGNENYALRERLTYLGLPAPLFTIDLDTEMGRRVGRDAFQRFFDCLEPAFGLQVTL
                     GQSNTVVVCPALTSHSELSEEALREAGITATTIRISVGDEDPRSLLAHLMKAAEIAIE
                     PAQEGFAAHFAPPEAVDELYESVYVDVHRRIAASRPRMQQLLA"
     gene            complement(2858..3289)
                     /locus_tag="VD737_05570"
     CDS             complement(2858..3289)
                     /locus_tag="VD737_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10061.1"
                     /translation="MPTLRTPDDFRLNLHRAATSPSRWWSRRPWADALTIVDTAAFVP
                     GRHNAAPLLEPTRKPRRARRGDPDTTWFHVRPATPSTAATRRGHHALSVGYGSLQLPA
                     TVRLVPDDEAERLEQARRGIAFRPRNGFLASTPLRRLPREA"
     gene            complement(3450..4379)
                     /locus_tag="VD737_05575"
     CDS             complement(3450..4379)
                     /locus_tag="VD737_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10062.1"
                     /translation="MRHLNGRAIGLVVLLLLPAAAAYRMGHDEFAHYCVLGTVLAFYL
                     GVQARQLATFGVLIPFVYAAAAVTATYTDGVAALIVAVAAATGAASSLGYHSGLLAVL
                     AAVLIGSYEPADADVVIGRAAEMLAGACYGVLLVYTVVRGLVARTIAVNSQTALGYSM
                     LLAVLVLVAWFMARVAHVAALWWLPLAVAAIGEPWLERTAGRAVGKLTVSLAATLVVL
                     TLLEPVTEPALRAACAVVLMLCLVLAGRGRPWLQGFLFTPVLVLLASDDRNHASAEYL
                     QATLVAFAVVAVFTVLGKWVLWTLRPDAGHATV"
     gene            4604..>5462
                     /locus_tag="VD737_05580"
     CDS             4604..>5462
                     /locus_tag="VD737_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXW10063.1"
                     /translation="MSRSRHCESRARPWKNALLLLGLAALVAACGGSSDGDGPSGSAC
                     GETARKQWVLDVVRDWYLYPETLPASVDLAAYATAEDLLDALTANARGLGKDRYFSYL
                     TTRSAENSLFGEGEFVGFGFGNRLDDGNRIFIFDVYAGSPAAEAGLLRGDEVIAVDGR
                     SVTDALASGTSFPDLLGPVEQGVSRTLTLRRGDASFDRTLTKRTVTLDPVPDDSGTAV
                     LPLAGTTGVGYLRLRSYISPADAQLRDAFAQFRAQNLSYYVIDLRYNGGGLLSTAELI
                     DDLLGGTLFS"
BASE COUNT          904 a         1952 c         1819 g          787 t
ORIGIN      
        1 cgaacacgaa gctcttcttc gccgagacga tcggcaaccc gaagatcaac gtgctcgaca
       61 tccgcaccgt cgccgacatc gcccacgaga gcggcgtgcc gctcatcgtc gacaacacga
      121 tcgcgacgcc gtacctcatc aagccgttcg agcacggcgc cgacatcgtg gtgcatgcgc
      181 tgaccaagta cctgggaggc cacggcaact cgatcggcgg cgcgatcgtg gattccggca
      241 agttcccgtg ggcgaagcac aaggcgcgct tccggcgcct gaacgagccc gacgtgagct
      301 accacggcgt ggtctacacc gaggcgctgg gcgaggccgc ctacatcggc cgtgcgcgcg
      361 tggtgccgct gcgcaacatg ggcgccgcgc tcagcccgtt caacgccttc ctgatcctgc
      421 agggcatcga gacgctcgcg ctgcgcatgg accgcatctg cgagaactcg ctcgtgatcg
      481 cgcggttcct gcagaagcac cccaaggttc ggtgggtcaa ctacgcaggc ctgcccgacc
      541 atccgagcca cggcctcgcc gagaaataca tgggcggacg ggcctcaggc atcctgacgt
      601 tcggcatcga cggcgggcgc gagggaggcg cgcgcttcca ggacgcgctg caactgttcg
      661 tgcggctggt caacatcggc gacgcgaagt ccctcgcgtg ccacccggcg agcacgacgc
      721 accgccaact caacgccgcc gaactcgagc gcgcgggcgt cagcgaggac atggtgcggc
      781 tgtcggtcgg catcgagcac gtcgacgacc tgatcgcgga cctcgagcag gcactcgccg
      841 ccgcctgagg aagtggctag tggctagtgg ctggtggcta gtgcggagcg gctgcgctgc
      901 ggaccgacgc agtcggttct tcactagcca ctgaccactg accactgacc actgaccgct
      961 ggccacttct tccgcctgtt accatcccgc gatgaagact ccctcctccg atttcgcgct
     1021 cgcgcgcgaa ctgagcccac gccgcaacac cacggacgcg gacgacgtcc cggcgctggt
     1081 caccgagcag ctgcgtcatt tctcgatcga tccgcagacg gacttcgggc gccggctcgg
     1141 cgagctggcc ggccacctgt acgccggcaa tgttgccgcg caccagctgt gggaagtggc
     1201 gatgcgcgaa ctcgccgcgc tcgaccagcg cgaccgggtc gcacgcttca atgccaagcg
     1261 gttcctctgc ttccagctcg cgaagatcct cgacacgctg cagaacccgc tgcgcaagac
     1321 gtaccagtcg ctgctgcacg acccgtcgca ggcggccgtc aagggtccct acccgatctt
     1381 cgacaacgtg actgccctgt tcagcgcgac gcccgtcatc gcgcgcacgg ccacgtacct
     1441 ctatgcctgc accgagtggg tcgaggacgc gttcaagggc cgcgagccgc tgctcgagat
     1501 ctattcgcgc ctgctgaacc cgacctcgat cagcctcgcg aaccacgtcg tcgacctcga
     1561 ggcggggtcg ctcgccggcg aatacctcgc ctggaacttc aactccggca tggccgcgat
     1621 cgacacgacg ctggccaacc tcgtcggagc gcaggacgtg gtgctcgcga gccgcaacgt
     1681 ctacggcggc acgtaccagc tgctccacga ctggtacggc aagaagagca acctcgacgt
     1741 ggccgtcgag tggttcgacg gcttcacggt cgaggacttc cgtcgcgccc tcgacggcgt
     1801 gcgggtgaag caccgcacgc gcctcgagcg tggccgcaac gtctacgtgt tcatcgagtc
     1861 gccgtgcaac ccccacggct acgtgctcga cgtcccggcg atctgtcgcg cggcgcacga
     1921 gcgaggccac acggtgatcg tggacgcgac cgtcggcacg ccggtgctgc agccggtgct
     1981 gcgacgcaag gatccgctcg agcggcccga cttcgtgatc cactcgtaca ccaaggacct
     2041 tgccggcgcc ggcacgacca cggccggcgt ctgcatcggt cgcaacgagc gcatgttcat
     2101 ccccaagggc gacaccgtca cggcgccggg gcccgacggc aagccgcgca cctacgtctg
     2161 ggacgagagc ctgttctgga acgtctacta cgtgaagggc gcgttcctcg acgcggacaa
     2221 ggcgttcgag gtcatcaacg gcatgcgcac ggtggaactg cgcgtgctgc agaaggcgat
     2281 caacacgatc gtgctcgccc gtgcgctcgc ccggcacccg gccttcggcg tgcgttgcgg
     2341 cgcggtcgaa ggcaacgaga attacgcgct gcgcgagcgg ctgacctacc tcgggctgcc
     2401 ggcgccgctc ttcacgatcg acctcgacac cgagatggga cgccgggtgg gccgcgacgc
     2461 gttccagcgc ttcttcgatt gcctcgagcc cgcgttcggc ctgcaggtga cgctcggcca
     2521 gtcgaacacc gtcgtcgtgt gccccgcgct caccagccac tcggagctgt cggaggaggc
     2581 gctccgcgag gccggcatca cggcgacgac catccgcatc tcggtcggcg acgaggatcc
     2641 gcgctcgctg ctcgcgcacc tgatgaaggc ggcggaaatc gcgatcgagc ccgcacaaga
     2701 ggggttcgcg gcacacttcg cgccgccgga ggccgtcgac gagctctatg aatcagtgta
     2761 cgtcgacgtg catcgacgga tcgccgcgag ccgtccccgg atgcagcaac tgctggcgtg
     2821 atccgcaaac gcctcgcgcg gcgtcacgcg cgccgattca ggcttcgcgg ggcagtcgcc
     2881 tgagcggcgt cgacgcgagg aagccgttgc gaggtcggaa ggcgatgccg cggcgtgctt
     2941 gctcgaggcg ttccgcttcg tcgtccggca cgagcctgac ggtggccggg agctgcaagg
     3001 agccgtagcc cacgctcagg gcgtggtgcc cccgtcgcgt cgcggccgtg gacggcgtgg
     3061 ccggccgcac gtggaaccag gtcgtgtcgg ggtcgccccg cctggcccgg cgcggcttgc
     3121 gcgtcggttc gagcagcggc gccgcgttgt ggcggcccgg cacgaaggcg gcggtgtcca
     3181 cgatcgtcag ggcgtcggcc cagggccggc gcgaccacca gcgcgagggg ctcgtggcgg
     3241 cgcggtgcag gttcaggcgg aaatcgtcag gcgttcgcaa cgtcggcata cgtcgtcatc
     3301 gtcttcacgc gcgtgcgtgg aaaagtgggt cgatcgatcg agtcgtgacg atagccaacg
     3361 acgtcgccgc gaaatgtgcg acgcatccgg tttcgacgcg tcgatcctcg ctcggccgtg
     3421 cggggtcgca ccgcgccggc gccagtcgct cacacggtgg cgtgacccgc gtccggccgc
     3481 agcgtccaga gcacccactt gccgagcacc gtgaacacgg cgacgaccgc gaacgcgacg
     3541 agcgtggcct gcaggtactc ggcggacgca tggttgcggt cgtccgaagc gagcagcacc
     3601 agcacgggcg tgaacaggaa cccctgcagc cacggccggc cgcgcccggc gagcaccagg
     3661 cacagcatca ggacgaccgc gcaggccgcg cggagggcgg gttcggtgac gggttcgagc
     3721 agggtcagca ccaccagcgt ggccgcaagc gagaccgtga gcttgccgac cgcgcgaccg
     3781 gccgtgcgct cgagccaggg ctccccgatc gccgcgacgg cgagcggcag ccaccacagg
     3841 gcggccacgt gggcgacccg cgccatgaac cacgcaacga ggaccagcac cgccagcagc
     3901 atcgagtatc cgagcgccgt ctgcgagttg acggcgatcg tgcgtgccac gagcccccgc
     3961 accacggtgt acacgagcag cacgccgtag caggcccccg cgagcatctc cgcggcgcgg
     4021 ccgatcacga cgtcggcgtc ggcaggctca taggagccga tcagcaccgc cgcgagcacg
     4081 gccagcagcc ccgagtggta cccgagcgag ctcgccgcac cggtggcggc cgcgacggcc
     4141 acgatcaggg cggcgacacc gtcggtgtag gtggccgtga cggcggcggc ggcatagacg
     4201 aacgggatca gcaccccgaa cgtcgcgagc tggcgcgcct gcacgccgag gtagaacgcg
     4261 agcacggtgc cgagcacaca gtagtgcgcg aattcgtcgt ggcccatgcg gtacgccgct
     4321 gccgccggca gcagcagcag gaccaccaga ccgattgctc gcccgttcag gtgtcgcatg
     4381 tgcctcgaca gcgaacgccg ctttcccgaa cacccccggc tcgaccgatc gcctgcaagt
     4441 tcactgccat cagctgacca ccagcgaaaa caatcgctta cggcccgctc gggtacgctt
     4501 cgcccatgat atgtaaaaaa ccccgacgag ggtccctcgc cgggctctgg ccgccggaac
     4561 ccaccatcgg ccacacttcg ccacccgccg acccaggtct gccatgtccc gatcccgtca
     4621 ttgcgagtcg cgcgctcgtc cctggaagaa cgccctgctg ctcctcggcc tcgcggcgct
     4681 cgtcgccgcg tgtggcggaa gcagtgacgg cgatggcccg tccggcagcg cctgtggcga
     4741 aacggcgcgc aagcagtggg tcctcgacgt cgtacgagac tggtatctct atcccgagac
     4801 cctgcccgcc agcgtcgacc tcgctgcgta cgcgacggcg gaagacctgc tcgacgcact
     4861 gacggcgaac gcccgcggcc tgggcaagga tcgctacttc agttacctga ccacccgctc
     4921 ggccgagaat tccctgttcg gcgaaggcga gttcgtcggc ttcggcttcg gcaaccgcct
     4981 ggacgacggc aaccgcatct tcatcttcga cgtctatgcc ggcagtccgg ccgcggaggc
     5041 cggcctgctg cgaggcgacg aagtgatcgc cgtcgatggt cgatcggtga ccgatgcgct
     5101 cgcgagcggg acgtcgttcc cggacctgct cggccccgtc gagcagggcg tgagtcgcac
     5161 gctgacgctc cgacgcggtg acgcgagctt cgaccgcacg ctgaccaagc gcacggtgac
     5221 gctcgacccg gtgcccgacg actcaggcac ggcggtgctg ccgctcgccg gcacgaccgg
     5281 cgtcggctac ctgcgactgc gcagctacat ttcacccgcg gacgcccagt tgcgcgacgc
     5341 gttcgcgcaa ttccgtgcgc agaacctcag ctactacgtc atcgacctgc gttacaacgg
     5401 tggcggcctc ttgagcaccg ccgagctgat cgacgacctg ctcggcggca cgctcttcag
     5461 ca
//
LOCUS       DASPFR010000219         6765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_428055, whole genome shotgun sequence.
ACCESSION   DASPFR010000219 DASPFR010000000
VERSION     DASPFR010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6765
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428055"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VD737_05585"
     CDS             <1..569
                     /locus_tag="VD737_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXW10064.1"
                     /translation="EYPTYDEGDGDYSFVGHVGYRVGRYFAVEAGFIDAGTIGWDKDF
                     VWMPELGGYYRNEVDFEATAPELSVVGILPFLQRWEAYGRIGAGFWSGDAEQTLTDVS
                     TGDVVHRHDSDDGVGIVVGLGLGFSITPSLHMRFEYQALWIDGDALNVDSETTLDTVA
                     LELQYRFGARRAATPAATAPGAGEAPDR"
     gene            729..2666
                     /locus_tag="VD737_05590"
     CDS             729..2666
                     /locus_tag="VD737_05590"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HXW10065.1"
                     /translation="MHDTDDIGSNESGNPHLQTMIEARVERRTFLKGTLASAATGFLG
                     TAALAPLRASATPGNVPTPRLRFAPVAPSTDDTVHVPDGYSWHVLAPWGTPLLPGAPD
                     FAEDASNSAADQALQVGFNHDGMHYFPLPVFGDSRGLLVVNHEYTDASQIYSAVQGST
                     ITADAAGREKVAKALAGHGVSVMAIQKGRDGRWSIVRDDDRNRRITGTTPMAFSGPVA
                     ATHPMLASVITPSPLGTLNNCAHGTTPWGTYLACEENWNGYFGTDDTSWVATPLEARY
                     GVSRAGFGYNWHKAEARFDLALNRNELNHFGWVVEIDPFAPTAQPVKRTALGRTKHEG
                     ATCTESKGRVVVYSGDDENGDYLYKFVGSAPWRQVRAQGLSPLDHGTLYVAQFDADGG
                     GRWLPLVHDVGPLTAANGWADQADVLIRTRLAADAVGATRLHRPEWVAVHPHSKEVFV
                     TLTNGSGNGSAVNSSRDPNPYGHIVRFREADGSDTHFAWDVFLLAGDPAYDPRVPADQ
                     PVFGSPDGIWVDRSGIVWVQTDISNSSQNLASRGYDRIGNNAVLAADPDTSDVRRFMT
                     GPRGCEITGITMTPDQRTLFVNIQHPGESTTYWNNQFGAPTTSNPSTVSTWPFGTAAN
                     PRRPRPATVVIQRRDGGRIGS"
     gene            2888..4396
                     /locus_tag="VD737_05595"
     CDS             2888..4396
                     /locus_tag="VD737_05595"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HXW10066.1"
                     /translation="MTRHRTVLAAVDLGTNSFHMVVARQANGQLVIIDRLREMVRLGE
                     GLGSDGRLDPVVAARALACLDRFGHRLLHVQANCVRVVGTSALRRVRRRDAFLDKARR
                     AIGHPIEVISGREEARLIYSGVVRTLPRTPGRRLVVDIGGGSTELIIGKGLRPLELES
                     LKLGCVTLSADHFGDGKLTAGRFERARLAVRQEIEPIRRAFLGRGWDTVAGSSGTVRA
                     LFETLRELDPHCSAITRAGLEEMLDLFVSYGHVSRLPFTTLGDDRRPVIAGGLAVLAE
                     ILSELGIRQMRVADGAMREGVLYDLVGRLTSEDAREQTVRSMQARYDVDRRQAARVEE
                     TAIALLRQVRKPWKLHDPLAERVLRWSARLHEIGLDVAHSGYHRHGAYLLENADMPGF
                     PREEQRLLARLVRWHRRRLELEDLTDLGPEWQFMAERLIILLRLAVLLHRNRGGANPP
                     RFAAVPGRHGLTLRFRLRSLRRHPLTAADLAQEQRHLEEQGWQLRIVMARPA"
     gene            4444..5277
                     /locus_tag="VD737_05600"
     CDS             4444..5277
                     /locus_tag="VD737_05600"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HXW10067.1"
                     /translation="MRRALLRDVATASEPVAVHRALGAEAVHETRKRIKRCRAVLRLL
                     RPALDDGDFAACDRALRRAGKALSRARDAAVASRLQAELGSLAAGRRKAPSRGPGPAG
                     PMASAADVARAHRSLQSAADSLALAKLHRRGWSTLGPGVRTVYRGGRRRVPVEAQAAS
                     GAALHDWRRHVKRYWHVLEVFSALNPERLGAVIGIARRLSDVLGEEHDLAMLDARLRA
                     RRDPPDDVLRLVAERRAKLTRRAVTLGTKLYREPSKTIERELHRDWDRWRERARTSAL
                     G"
     gene            complement(5311..5529)
                     /locus_tag="VD737_05605"
     CDS             complement(5311..5529)
                     /locus_tag="VD737_05605"
                     /inference="COORDINATES: protein motif:HMM:NF023507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3562 domain-containing protein"
                     /protein_id="HXW10068.1"
                     /translation="MNDPLSIPNMPGLDVDPRVVESLASHYGLPVGDVEVILREELDD
                     LAANARIGTFVMVLATSRARARLQAGTR"
     gene            complement(5657..>6765)
                     /gene="ppk1"
                     /locus_tag="VD737_05610"
     CDS             complement(5657..>6765)
                     /gene="ppk1"
                     /locus_tag="VD737_05610"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HXW10069.1"
                     /translation="GGDIFAAIHAGDVLVHHPYESFDASVERFIRDAADDPAVTTIKM
                     TVYRLGDDTPFVRSLIKAAENGKQVACLVELKARFDEERNLHWAEQLQKAGDHVIYGM
                     RGLKTHTKLALVVRREGDGLRSYAHVGTGNYHVKTARLYTDFGLFTCDPVLTRDIVTL
                     FHHLTGYSRAPRFEKLLVAPLNMRGRFVDLIGREAANAQAGRPARIVAKLNQLEDPPI
                     CAALCEASNAGVPIHLIVRGFCCLKPRVAGFTENLQVRSIIGRFLEHARVFHFANGHE
                     DPLDGDFYLGSADWMDRNLSRRVEAVAPVENRHLRERLWEVLQVQLADERNAWIMQPD
                     GTYQQCSPSSRAPRMAREGSHATLMKRTRQRVRR"
BASE COUNT         1061 a         2363 c         2287 g         1054 t
ORIGIN      
        1 gcgaataccc cacctacgac gaaggcgacg gcgactactc tttcgtgggc cacgtgggat
       61 atcgcgtcgg gcgctacttc gccgtcgagg ccggcttcat cgacgcaggc acgatcggat
      121 gggacaagga tttcgtctgg atgcccgaac tcggcggcta ttaccgcaac gaagtcgact
      181 tcgaggccac ggcgcccgaa ctctccgtgg tgggcatcct gccgttcctg cagcgctggg
      241 aggcctacgg tcgcatcggt gccggcttct ggagcggcga cgccgagcaa acgttgaccg
      301 acgtgtccac cggtgacgtg gtgcaccgac acgacagcga cgacggcgtc gggatcgtcg
      361 tcggtctcgg cctcggcttc agcatcaccc cgtcgctgca catgcgcttc gagtaccagg
      421 cgctctggat agacggtgac gcgctcaacg tcgattcgga gacgacgctc gacacggtcg
      481 cgctcgagtt gcagtaccgc tttggcgcgc gccgggcggc gacgccggca gcgactgcgc
      541 ccggcgcagg cgaagcaccg gaccgctgac gtccagtcgc caacggcgcc gggctgttca
      601 aattcatcga tccgtcacgc cagcgtcatc tgcggacggt gcaatggtgc ctgcgacagc
      661 gggcgcctgc attgttcgtc ctccgtcgcg tcaagaacac ccatacgcac gctacggaga
      721 tcgacgacat gcacgatacg gacgacatcg gctcgaacga gtccggcaat ccccacctcc
      781 agacgatgat cgaggctcgc gtcgagcgac gtacgttcct gaagggcacg ctcgcctccg
      841 cggccaccgg cttcctgggc accgcggccc tcgcgccgtt gcgagcctcc gccacgccgg
      901 gtaacgtgcc cacgccgcgg ctccgctttg cgccagtcgc ccccagcacc gacgacacgg
      961 tgcacgtgcc cgacgggtac agctggcacg tgctcgcgcc gtggggaacg ccgttgctcc
     1021 cgggcgcccc ggacttcgcc gaggacgcgt ccaactccgc ggccgatcag gccctgcagg
     1081 tcggcttcaa ccacgacggc atgcactact tcccgctgcc ggtgttcggt gactcgcgcg
     1141 gcctgctggt cgtgaaccac gagtacaccg acgccagcca gatctactcc gccgtgcagg
     1201 gctcgacgat caccgccgac gccgcggggc gcgagaaggt cgcgaaagcg ctggcgggcc
     1261 acggcgtgag cgtcatggcg atccagaagg gtcgcgacgg tcgctggtcg atcgtgcgtg
     1321 acgacgaccg caatcgccgc atcaccggca cgaccccgat ggcgttctcc ggacccgtcg
     1381 ccgcgaccca tccgatgctc gcctccgtga tcacgccgag cccgctcggc acgctcaaca
     1441 actgcgccca tggcacgaca ccctggggca cctacctcgc gtgcgaggaa aactggaacg
     1501 gctatttcgg gaccgacgac acctcctggg tcgccacgcc cctcgaggcg cgttacggcg
     1561 tctcacgcgc gggcttcggc tacaactggc acaaggcgga agcccggttc gacctggcgc
     1621 tgaaccgcaa cgagctcaac cacttcggct gggtcgtcga gatcgacccg ttcgccccga
     1681 ctgcgcagcc ggtcaagcgc acggcgctcg gtcggaccaa gcacgagggc gcgacctgca
     1741 cggaaagcaa gggccgcgtc gtcgtctaca gtggagacga tgagaacggt gactacctgt
     1801 acaagttcgt cggtagcgcg ccctggcggc aggtccgcgc gcagggcctg agcccgctcg
     1861 accacggcac gctctacgtc gcgcagttcg acgccgacgg cggcggccgc tggctgccgc
     1921 tcgtccacga cgtcggtccg ctgacggccg ccaacggctg ggcggaccag gcggacgtgc
     1981 tgatccgcac gcgcctggcc gcggacgcgg tcggcgccac gcggctgcac cgccccgagt
     2041 gggtggcggt gcacccgcat tcgaaggagg tcttcgtgac cttgaccaac ggcagcggca
     2101 acggctccgc cgtcaacagc tcgcgcgatc cgaacccgta cggtcacatc gtgcgattcc
     2161 gcgaggccga tggcagcgac acgcacttcg cctgggacgt cttcctgctc gccggcgatc
     2221 cggcttacga cccgcgcgtg cccgcagacc agccggtctt cgggtcaccc gacggaatct
     2281 gggtcgatcg cagcggcatc gtctgggtgc agaccgacat ctcgaactcg tcgcagaacc
     2341 tggcgagccg cggttacgat cgcattggca acaacgcggt gctcgcggcc gaccccgaca
     2401 cgtcggacgt gcgtcgcttc atgacgggac cgcgcggctg cgagatcacc ggcatcacca
     2461 tgacacctga ccagcgcacg ctgttcgtga acatccagca tcccggcgag tcgacgacct
     2521 actggaacaa ccagttcggc gcgcccacga cctcgaaccc gtccaccgtg agcacgtggc
     2581 cgttcggcac ggcagcgaac ccgcgtcggc cccgaccggc gacggtggtg atccagcgca
     2641 gggacggcgg gcgcatcggc agctgatcga gacggctgcc cgccttctcg gcaggtgtgc
     2701 ccgcggcgtc cagacacgac gccgcgggca cgtgttcatg cggtgcgcgt caggcatgaa
     2761 cgaacctggc tcggtgacgt cgtcatccaa ctgtggcgtg gtcgggggtg acgcggtaag
     2821 ccacgaacac cgatcgcatg gtcggatggc gaggcgcaga ataggccgcc gtccagccag
     2881 tgactccatg acgcgccatc gcaccgtact cgccgccgtc gacctcggca ccaacagctt
     2941 ccacatggtc gtcgcgcggc aggccaacgg acagctggtc atcatcgacc ggttgcggga
     3001 gatggtcagg ttgggtgaag gcctcggttc cgacggacgg ctcgaccccg tggtggctgc
     3061 gcgcgccctc gcgtgcctgg atcgattcgg tcatcgactg ctgcacgtgc aggccaactg
     3121 tgtgcgcgtg gtgggcacga gcgcgctgcg ccgcgtgcgt cgccgggacg cgttcctcga
     3181 caaggcgcga cgcgccatcg gccacccgat cgaagtgatc tccggccggg aagaggcgcg
     3241 actgatctac tcgggggtcg tgcgcaccct gccacgcacg ccgggccgtc gactcgtggt
     3301 cgacatcggc ggtggcagca cggagttgat catcggcaag ggtctccgtc ccctggaact
     3361 ggagagcctg aaactcggct gcgtcacgtt gagcgccgac cacttcggcg acggcaaact
     3421 cacggcggga cgcttcgagc gagcacggct ggccgtgcgg caggagatcg agccgatacg
     3481 ccgcgcgttc ctcggtcgcg gctgggatac cgtcgccggc agctctggaa cggttcgcgc
     3541 gctgttcgag acgctgcgcg aactcgaccc gcactgttcg gcgatcacgc gagccggact
     3601 cgaagaaatg ctcgatctgt tcgtgtccta cggccacgtc tcgaggctgc cgttcacgac
     3661 gctcggcgac gatcgacggc cggtcatcgc gggcgggctg gcggtgctcg ccgagatcct
     3721 gtccgagctc ggcattcgcc agatgcgcgt cgcagacggc gccatgcgcg aaggcgtact
     3781 gtacgacctc gtcggccgcc tgacctcgga ggacgcacgc gagcagaccg tgcgttccat
     3841 gcaggcccgt tacgacgtcg accgacgcca ggcagcgcgt gtcgaggaaa cggccatcgc
     3901 gctgctgcgc caggtcagga agccatggaa gctccacgat ccgctggccg agcgcgtgct
     3961 tcgctggtcg gcccggctcc atgaaatcgg tctcgacgtc gcgcacagtg gctatcaccg
     4021 tcatggcgcg tacctgctcg agaacgccga catgcctgga tttccgcgcg aggagcaacg
     4081 cctgctggcc cggcttgttc gctggcaccg ccgccgcctc gagctcgagg acctgacgga
     4141 cctgggcccg gagtggcagt tcatggccga acgcctgatc atcctgttgc gccttgccgt
     4201 gctgctgcat cgcaatcgcg gcggcgcgaa cccgccccgg ttcgccgcgg ttcccggtcg
     4261 tcatggcctc acgctgcgat ttcggctgcg ttcgctgcgc cgccatccgc tcacggcggc
     4321 cgacctggcc caggaacaac ggcatctcga ggagcaaggc tggcagctgc gcatcgtcat
     4381 ggcccgcccg gcatgactcg acgggcagcc ccgccgggcg cgccagtcac cagtgtggcc
     4441 gccatgcgtc gtgccttgct ccgcgacgtc gccaccgcga gcgagccggt ggcagttcat
     4501 cgcgcgctcg gtgctgaggc ggttcacgag acccgcaagc gcatcaagcg ctgccgcgcg
     4561 gtgttgcgat tgctgcgacc cgcgctcgac gacggcgatt tcgcggcctg tgaccgagcc
     4621 ttgcgtaggg ccggcaaggc gctgtcgagg gctcgggacg cggccgtggc gtcgcgtctc
     4681 caggcggaac tcggttcgct cgccgccggc cggcgcaagg cgccgtcacg cggccccggg
     4741 cccgcaggac ccatggcctc cgcggcagac gtggcccgtg cgcaccgaag ccttcaatcg
     4801 gccgcggatt ccctcgcgct cgcgaaactg catcgtcgtg gctggtcgac gctgggcccc
     4861 ggcgtccgca cggtgtaccg aggcggccgt cgtcgcgtgc ccgtcgaggc gcaggccgca
     4921 tcaggcgcag ccctgcacga ctggcgcagg cacgtcaagc gctactggca cgtgctggag
     4981 gtgttttcgg cgctcaatcc cgagcgactc ggcgccgtca tcggcatcgc acgcagattg
     5041 tcggacgtgc tcggcgagga acacgatctc gcgatgctcg acgcgcgatt acgcgcccgt
     5101 cgcgacccgc cggacgacgt gcttcgcctg gtggccgagc gccgggcgaa actcacccga
     5161 cgcgccgtca cgctcggcac gaagctctat cgcgagcctt cgaagacgat cgaacgcgaa
     5221 ctgcaccggg actgggaccg gtggcgcgag cgtgccagga cgtccgcact cgggtgacgt
     5281 gccggggcgt cacgccctgc aggccccgcc ctatcgcgtg cccgcctgca acctcgcacg
     5341 ggcccggctc gtcgcgagca ccatgacgaa cgtgccgatg cgggcgttcg ccgccaggtc
     5401 atccagttct tcccgaagta tgacttcgac gtcgccgacg ggaagaccgt aatggctggc
     5461 cagggattcg acgacccgcg gatcgacgtc gaggccgggc atgttcggga ttgagagcgg
     5521 gtcgttcacg cagaccaatg ttacccgcgt gcgcggagcc ctttacgtcg gaaaatcact
     5581 agccgggact gtcgccattc tgcaaacgtg cgtgccggac agacgccggc ctcctgcggg
     5641 ccgtgcgcgg gcgccatcaa cgtcggacgc gctgtcgcgt tcgcttcatc agcgtcgcgt
     5701 gactgccttc gcgcgccatc cggggagccc ggctcgaagg gctgcactgc tgatacgtgc
     5761 catcgggctg catgatccag gcgttgcgtt cgtcggcgag ctgcacctgc agcacttccc
     5821 agagccgctc ccgtagatga cggttctcca ccggcgccac ggcttcgacc cgacgggaga
     5881 ggttccggtc catccagtcc gccgagccga ggtagaagtc gccgtcgagc gggtcttcgt
     5941 ggccgttggc gaagtggaaa acccgcgcgt gctcgaggaa gcgcccgatg atggaacgca
     6001 cctgcaggtt ctcggtgaat ccggcgacgc gtggcttcag gcagcagaag ccgcgcacga
     6061 tcaggtggat cggcacgccg gcgttcgagg cctcgcagag cgccgcgcag atcggcgggt
     6121 cctcgagctg gttcagcttc gccacgattc gggcgggccg ccccgcctgc gcgttcgcgg
     6181 cttcgcggcc gatcaggtcg acgaagcggc cgcgcatgtt cagcggcgcg acgagcagtt
     6241 tctcgaaccg tggcgcgcgc gagtagccgg tcaggtgatg gaacagcgtc acgatgtcgc
     6301 gcgtgagcac cgggtcgcag gtgaagaggc cgaagtccgt gtagagccgc gccgtcttga
     6361 cgtggtagtt gccggtgccg acgtgggcgt agctgcgcag gccgtcgccc tcgcgtctca
     6421 cgaccagcgc gagcttcgta tgcgtcttga gtccccgcat gccgtagatc acgtggtcgc
     6481 cggccttctg aagctgctcg gcccagtgca ggttgcgctc ctcgtcgaac cgcgccttga
     6541 gctcgacgag gcaggcgacc tgcttgccgt tctcggcggc cttgatcagc gagcgcacga
     6601 acggcgtgtc gtcaccgagg cggtagaccg tcatcttgat cgtcgtcacg gccgggtcgt
     6661 cggccgcgtc acgaatgaac ctctccaccg aggcgtcgaa gctctcgtac gggtggtgga
     6721 cgagaacgtc gccggcatgg atggctgcaa agatgtcgcc gcccg
//
LOCUS       DASPFR010000220         7111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1650037, whole genome shotgun sequence.
ACCESSION   DASPFR010000220 DASPFR010000000
VERSION     DASPFR010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7111
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650037"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VD737_05615"
     CDS             complement(<1..197)
                     /locus_tag="VD737_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10070.1"
                     /translation="MLSLDEVLRRLHAFYGPLAPPPHDPFMMYVWEVLNFHSVPVKRD
                     AALAALRRIPALTPDSIWKTVP"
     gene            complement(214..1593)
                     /locus_tag="VD737_05620"
     CDS             complement(214..1593)
                     /locus_tag="VD737_05620"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HXW10071.1"
                     /translation="MDRVIDFINLQRDRYVDELKTYLAIPSISALPQHAADVRRCAEW
                     TADELRRIGLQNVRLIETPGNPVVYGDWLGAPDAPTILFYGHYDVQPVDPLDKWVSPP
                     FEATVRDGEIYARGAADDKGQIFMHFKAVEAHLKQNGSLPCNIRIFIEGEEEVGSSHL
                     DDLVRSMKGELASDVVVISDSPMLERGVPSICYGLRGLAYFQIDVKGTNTDLHSGSFG
                     GAVANPAMVLAQILAQMKDRSGRIKIPGFYDDVRDLRPEEREEWRRLPFNETKYRKEI
                     GAPKLFGESGYTTLERVWARPTFEVNGLLAGFTGEGAKTVIPATAMAKVSMRLVPDQD
                     PNTVADLFEAYLKKVAPRTVEVELTRMQGGKAWMTEFDNRYVQAAARAIERGFGKAPV
                     FNREGGSIPVVSTFQQELNVPAVLFGVGLPDENAHAPNEKLDLTNFHNGVIASAYLYE
                     EIGRLNGAS"
     gene            complement(1788..2255)
                     /locus_tag="VD737_05625"
     CDS             complement(1788..2255)
                     /locus_tag="VD737_05625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HXW10072.1"
                     /translation="MAVSIGGRAVAVSLPGSQESGSAIAYCMASRFEDLVVWQRARKM
                     CSEILPILDAAARRRDFRLAQELNECALSVPANIAEGKLRRGNRQFAYFLRIAAGSNG
                     EVRSWLHLAADRSYVSADRAAALIEETNETGKMLESLIRRVSLDDEEPPDDAT"
     gene            complement(2340..3155)
                     /gene="rlmB"
                     /locus_tag="VD737_05630"
     CDS             complement(2340..3155)
                     /gene="rlmB"
                     /locus_tag="VD737_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016803977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HXW10073.1"
                     /translation="MKRGHDSSRQARSHAAAQASIAYGIHTVRALLTRHPQRVRQLWL
                     ADSRESAARLQELRELATAAGVAIRGVDGDELDRLAAGERHQGAVAEVAPRAGDPETQ
                     LEEALEALGDTPGLLLVLDGVTDPHNLGACLRSADAAGVAAVIVPRDRAAGLTPVVRK
                     VAAGAAESVPLVAVVNLARTLRELKERGLWLVGTAGDASSTLYDVDLTGPTVLVLGSE
                     GEGMRRLTREACDQLVSIPMAGAVESLNVSVATGIALFEAVRQRRAQRLAVSG"
     gene            complement(3152..5410)
                     /gene="rnr"
                     /locus_tag="VD737_05635"
     CDS             complement(3152..5410)
                     /gene="rnr"
                     /locus_tag="VD737_05635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HXW10074.1"
                     /translation="MRRTRNRRKSEPDHRPAKTKAQRGRTPVRLAKSADLAVLNVLRK
                     AGRPLRLDELRASFSASGAQQAEEVLADLVRRGEVVLNRRGQYCLREQLAGLTVGTVQ
                     AHRNGDGWLLPDDGSAQVYLPAHQMREVLHGDRVAARIEGPRYRGKAQGTLVEVLERR
                     TKEVVGRLHVEAGIAYLTPDNPRITHRVLVPSGELAGATSGQIVLVELIQQPSRTGGP
                     VGRIARVLGDHGAPGMETDIAIHSFGLPYEFPENVVAEAEAYGARIPQDAISGREDLR
                     EIGLVTIDGEDARDFDDAVWCERKGAGWRLIVAIADVAHYVRPASPLDVEARERGTSV
                     YFPNRVLPMLPEALSNGLCSLKPDEDRLCVCCELRVSEDGRITRSRFFEAVMRSSARL
                     TYREVGAFLEKPAARHDERLERLRERLLALHGVYKAFTRARSGRGALELDTPELKLKF
                     DEQGRVAALVEYPRNDAHRLIEECMIAANVAAARFLDRHRVPTLYRVHGLPELDRLET
                     LRTFLREFGLWLPAPDEITPEHLRDLLSKIGDRPDAGLISTAVIRSMPQAVYQPGNIG
                     HFGLALEHYAHFTSPIRRYPDLVVHRGIRQVLRDGDPQDLIAWHGAFPQLGQDCSVRE
                     RRADEATRSAVSWLKCYYMQDRVGEEYDGIVSGVVDFGLFVQLDGLQVDGLLHVSALG
                     ADYFGRDASGYRMVGRSSGKVFKLGDRVRVRVTNVSLDDRRIDFELAGEMQGDRRPSG
                     RYLRQGGRRRRG"
     gene            5495..5579
                     /locus_tag="VD737_05640"
     tRNA            5495..5579
                     /locus_tag="VD737_05640"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5529..5531,aa:Leu,seq:cag)
     gene            5706..6623
                     /locus_tag="VD737_05645"
     CDS             5706..6623
                     /locus_tag="VD737_05645"
                     /inference="COORDINATES: protein motif:HMM:NF006109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HXW10075.1"
                     /translation="MQFSHCRGLPMTYTTIDYTVTDRVLTLTLNRPERLNAFTVMMAD
                     ELEHAFRRASHDDDVGAIVVTGAGRAFCAGMDLSSSGNVFGLDETLQPTLTDMRERLD
                     DPAILRGVRDTGGRVTLAIYDCLKPVIGAINGAAVGIGATMTLAMDVRLAAEGAKIGF
                     VFGRLGIVPEACSSWFLPRLVGVSQALEWIYAAEPFDAAEAVRGGLVKAAVPADRLLD
                     EARALAHRFIDGRSPVAVAMARQLVWRNLAQPHPIEAHRVDSLAMWHLSQGDGAEGVR
                     AFLEKRPAQFTSKASTDLPGFAAEWMKAP"
     gene            complement(6662..>7111)
                     /locus_tag="VD737_05650"
     CDS             complement(6662..>7111)
                     /locus_tag="VD737_05650"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645449.1"
                     /note="catalyzes the formation of malate from glyoxylate
                     and acetyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HXW10076.1"
                     /translation="DGLLDIPLASYRKWSKEQVRREVENNAQGILGYVVRWIDQGVGC
                     SKVPDINNVGLMEDRATCRISSQHIANWLHHRIVTPERVMEVMRRMAAVVDRQNSGDP
                     VYRPMAPAFDGPAFQAACDLVFEGTKQPSGYTEPLLHRHRQVAKGRR"
BASE COUNT         1076 a         2493 c         2356 g         1186 t
ORIGIN      
        1 gggaccgtct tccagatcga atcgggcgtc agcgcgggga tccggcggag cgccgccagt
       61 gcggcgtcgc gcttgaccgg cacgctgtgg aagttcagca cctcccacac gtacatcatg
      121 aatggatcgt gcggcggcgg cgcgagcgga ccgtagaacg cgtgcagccg ccgcagcact
      181 tcgtccagtg acagcacgcg tcaggccctc cgctcaggac gctccattca gccggccgat
      241 ctcctcatac aggtacgccg acgcgatgac gccattgtgg aagttggtca ggtcgagctt
      301 ctcgttcggc gcgtgggcgt tctcgtccgg cagcccgacg ccgaacagca cggccggcac
      361 gttcagctcc tgctggaacg tcgacacgac agggatggac cctccttcgc gattgaagac
      421 cggcgccttg ccgaacccgc gttcgatcgc acgcgccgcc gcctgcacgt atcggttgtc
      481 gaactccgtc atccaggcct taccgccctg catccgggtg agttcgacct cgaccgtcct
      541 gggcgccacc ttcttcaggt acgcctcgaa caggtcggcg accgtgttgg ggtcctggtc
      601 cggcaccagg cgcatgctca ccttggccat ggccgtggcg ggaatcaccg tcttcgcgcc
      661 ctcgcccgtg aatcccgcga gcagcccgtt cacctcgaag gtcggccggg cccacacgcg
      721 ctcgagcgtc gtgtatccgc tctccccgaa gagcttcggc gcgccaatct ccttgcggta
      781 ctttgtctcg ttgaacggca gccgccgcca ttcctcgcgc tcctccggcc tcaagtcgcg
      841 cacgtcgtca tagaagccgg ggattttgat ccggccgctg cggtccttca tctgcgcgag
      901 aatctgggcg aggaccatcg cgggattcgc gaccgcaccg ccgaacgacc cggagtgcag
      961 gtcggtgttc gtccccttca cgtcgatctg gaagtacgcc agtccgcgga gcccgtagca
     1021 gatcgacggc actccacgct cgagcatcgg ggagtctgaa atcaccacga cgtcggatgc
     1081 gagctccccc ttcatgctgc gaaccaggtc gtcgagatgc gagctgccga cctcctcctc
     1141 gccttcgatg aagatgcgga tattgcaggg cagcgatccg ttctgcttca gatgcgcctc
     1201 gacggccttg aagtgcatga agatctggcc cttgtcatcg gccgcgccgc gggcgtagat
     1261 ctcgccgtcc cggacggtcg cctcgaaggg cggagacacc cacttgtcga gcggatcgac
     1321 gggctgcacg tcgtagtgcc cgtagaagag gatcgtgggc gcgtccggcg caccgagcca
     1381 gtcgccataa accaccggat tgcccggcgt ctcgatcagg cggacgttct gcaggccgat
     1441 ccgtcgcagc tcgtcggccg tccactcggc gcagcggcgc acgtcggccg cgtgctgcgg
     1501 cagcgcgctg atgctgggaa tcgcgaggta cgtcttcagc tcgtcgacgt agcgatcgcg
     1561 ctggaggttg atgaagtcga tgacgcggtc cattttttct tcgtttagct gttagcgtta
     1621 gcggttagcg attaggctgt cttgctgttg gctgttggct gttggctgtt ggctgttggc
     1681 aaccgattat tgccgtggcg gttggccgct attggctggg ggcaaaatgg tgacggttgc
     1741 accgtatcgc cagcaatggc tgatcgccgg ctacgtcccg aaattggcta cgtagcgtca
     1801 tcgggaggct cttcgtcgtc cagcgacact cgccggatga gactctcgag catcttgccg
     1861 gtctcgttgg tctcctctat cagcgccgcg gcgcgatcgg cagagacata cgagcgatcg
     1921 gccgccaggt gcagccagga tcgcacttcg ccgttcgatc ccgccgcgat tcgcagaaaa
     1981 taggcgaact gtcgattgcc cctcctcagc tttccctcgg caatatttgc cggcaccgag
     2041 agcgcgcatt cgttcagttc ctgcgcgagc ctgaagtcgc gtctgcgagc cgcagcgtcg
     2101 agaatcggaa gaatctcact gcacatctta cgcgcacgct gccacaccac aagatcttcg
     2161 aatcgcgagg ccatgcagta agcaatggcc gaaccagact cctgcgagcc cggcaacgaa
     2221 acagcgacgg cgcgcccacc gatgctcacg gccatcaagc agcagccagc agccaaccgc
     2281 taaccgcagc agggctaacc gccaacaggt aaccgcagct aaccgctaac cgctaaccgc
     2341 taaccgctga ccgctaaccg ctgggcgcgc cgttgccgca cggcctcgaa cagcgcgatg
     2401 ccggtcgcaa cggacacgtt caggctttcg acggcgcccg ccatgggaat cgacacgagc
     2461 tggtcacacg cttcgcgggt caggcggcgc atgccctcgc cttccgagcc cagcaccagc
     2521 acggtcggcc ccgtgaggtc gacgtcgtag agcgtgctcg acgcgtcacc ggccgtgccg
     2581 accagccaca gcccccgctc cttgagttcg cgcagcgtcc gcgccaggtt gacgacggcg
     2641 accagcggca cgctctccgc cgcgcccgcc gcgaccttgc gcacgacggg ggtcagcccg
     2701 gccgcgcggt cgcgcgggac gatcaccgcg gcgactccgg ccgcgtctgc gctgcgcagg
     2761 cacgcgccca ggttgtgcgg atccgtcacg ccgtcgagta cgagcagcag gcccggcgtg
     2821 tcgccgagcg cctcgagcgc ctcttcgagc tgcgtctccg gatcgcccgc gcgcggtgcc
     2881 acctcggcga ccgcgccctg gtggcgctcg cccgcggcga ggcggtcgag ctcgtcgccg
     2941 tcgaccccac ggatggccac gcctgcggcc gtggcgagct cacgcagctc ctgcaaccgc
     3001 gctgccgact cgcggctgtc cgcgagccag agctgtcgca cccgctgcgg atggcgcgtc
     3061 aacagcgcac gcaccgtgtg gatgccgtag gcgatgctgg cctgcgccgc cgcgtgcgac
     3121 ctcgcctgtc gtgacgagtc gtggccgcgc ttcatccgcg tcgcctccgt ccaccctgcc
     3181 gcaggtagcg accggacggg cgccgatcgc cctgcatttc gccggcgagt tcgaagtcga
     3241 tgcggcggtc gtcgagcgag acgttggtca cgcgcacccg cacacggtcg ccgagcttga
     3301 acaccttgcc ggaactgcgg ccgaccatgc ggtacccgga agcgtcgcgg ccgaagtaat
     3361 cggcgccgag cgcggacacg tgcagcaggc cgtccacctg caggccgtcg agctgcacga
     3421 acaggccgaa gtcgaccacg cccgagacga tgccgtcgta ttcctcgccg acgcggtcct
     3481 gcatgtagta gcacttcagc caggagacgg cgctgcgcgt ggcctcgtcg gcacgtcgtt
     3541 cgcgcaccga gcagtcctgg cccagctgcg ggaacgcgcc atgccatgca atcaggtcct
     3601 gcgggtcgcc gtcgcgcagc acctgccgga tgccacggtg caccacgagg tcggggtagc
     3661 gccggatcgg ggacgtgaag tgcgcgtagt gctcgagcgc gaggccgaag tggccgatgt
     3721 tgccaggctg gtagacggcc tgcggcatcg agcggatcac ggcggtcgag atcaggccgg
     3781 cgtccggccg gtcgccgatc ttcgacagca ggtcgcgcag gtgctcgggc gtgatctcgt
     3841 cgggtgccgg cagccagagc ccgaactcgc gcaggaacgt ccgcagcgtc tcgagcctgt
     3901 cgagctcggg caggccgtgc acgcggtaca gcgtcggcac gcgatgacgg tcgaggaacc
     3961 gggccgcggc gacgttggcc gcgatcatgc attcctcgat cagccggtgc gcgtcgttgc
     4021 gcgggtactc gaccagcgcc gcgacgcgac cctgctcgtc gaacttgagc ttgagctccg
     4081 gcgtgtcgag ctcgagggcg ccgcggcccg agcgcgcgcg cgtgaacgcc ttgtagacgc
     4141 cgtgcagcgc gagcagccgc tcgcgcagcc gttcgagccg ttcgtcatgg cgcgccgcgg
     4201 gcttctcgag gaacgcgccg acctcgcgat acgtgagacg cgccgaggac cgcatcactg
     4261 cctcgaagaa tcgcgatcgc gtgatgcggc cgtcctcgct cacgcgcagc tcgcagcaca
     4321 cgcataaccg gtcttcgtcg ggcttgagcg agcagagccc gttcgagagc gcctccggca
     4381 gcatcggcag cacgcgattg gggaagtaga ccgacgtgcc gcgctctcgc gcctcgacgt
     4441 cgagcgggct ggcggggcgc acgtagtggg cgacgtcggc gatcgcgacg atcagccgcc
     4501 agccggcgcc cttgcgttcg caccagactg cgtcgtcgaa gtcgcgcgcg tcctcgccgt
     4561 cgatcgtgac gagcccgatc tcgcgcaggt cctcgcgtcc cgagatcgcg tcctgcggaa
     4621 ttcgtgcgcc gtaggcctct gcctcggcca cgacgttttc cggaaactcg tacggcagcc
     4681 cgaacgaatg gatcgcgatg tcggtctcca tgccgggcgc gccgtggtcg ccgagcacgc
     4741 gcgcgatgcg acccaccggc ccgcccgtcc tcgacggctg ctggatcaat tccaccagca
     4801 cgatctggcc cgacgtcgcg ccggcgagtt cgccgctcgg cacgagcacg cgatgcgtga
     4861 tgcgcggatt gtcgggcgtc aggtaggcaa tgccggcctc gacgtgcagg cgcccgacga
     4921 cctccttcgt gcgtcgctcg agcacctcga cgagggttcc ctgcgccttg ccgcgatacc
     4981 ggggcccttc gatgcgcgcg gcgacgcggt cgccgtgcag cacctcgcgc atctggtggg
     5041 ccggcaggta gacctgcgcc gagccgtcgt ccggcagcag ccacccgtcg ccgttgcgat
     5101 gcgcctggac cgtgccgacc gtgaggcccg ccagttgttc gcgcaggcag tactggccgc
     5161 gccggttgag caccacctcg ccccgccgga cgaggtccgc gaggacctct tcggcctgct
     5221 gcgcgccaga cgcgctgaag ctcgcccgca gctcgtcgag ccgcagcggg cgcccggcct
     5281 tgcgcagcac gttgagtacg gccaggtcgg ccgacttcgc gagcctgacc ggggtccgtc
     5341 cgcgctgggc cttggtcttg gccggccgat ggtcgggctc cgacttgcgt ctgttgcggg
     5401 ttcttctcat tggctcgtta gattgacagt ccaggggtcc gccagtaaat tgcccggcct
     5461 tccagcccgg cctcggtgcc cggcccgcga ccatgcccag gtggcggaat tggtagacgc
     5521 actagtttca ggtactagcg ggtaacaccg tggaggttcg agtcctctcc tgggcaccac
     5581 tccgccgcgc tcgggcgtga atcggggcgg ccgacctgca cctcggtcgt ccatccccgt
     5641 gactttagca ctgcatccgc tccgcaggtc gccagtccac gagccagcga cgacacgcga
     5701 ctagaatgca gttttcgcat tgccgcgggc tgcccatgac gtacacgacg atcgactaca
     5761 cggtgaccga ccgtgtcctc acgttgacgc tcaatcgacc cgagcgcctc aacgcgttca
     5821 ccgtgatgat ggccgacgag ctggaacacg cgttccgtcg cgcgagccac gacgacgacg
     5881 tcggcgcgat cgtcgtcacc ggcgccggcc gggccttctg cgccggcatg gacctctcga
     5941 gctcgggcaa cgtgttcggg ctcgacgaga cgctgcagcc cacgctcacc gacatgcgcg
     6001 agcgcctcga cgacccggcc atcctgcgcg gcgtgcgcga caccggcggg cgcgtcacgc
     6061 tcgcgatcta cgattgcctg aagcccgtca tcggcgcgat caacggcgcg gccgtcggca
     6121 tcggcgcgac gatgaccctg gccatggatg tccggctcgc ggccgagggc gcgaaaatcg
     6181 gcttcgtgtt cggccggctc ggcatcgtgc cggaggcgtg ctcgagctgg ttcctgcccc
     6241 gcctggtcgg cgtctcgcag gcgctcgaat ggatctacgc ggcggagccg ttcgacgcgg
     6301 ccgaagccgt gcgcggcggg ctcgtcaagg cggcggtgcc ggcggatcga ttgctcgacg
     6361 aggcacgggc gctcgcgcac cgcttcatcg acggtcgttc gcctgtcgcc gtcgcgatgg
     6421 cccggcagct ggtctggcgc aaccttgcgc agccgcaccc gatcgaggca catcgcgtcg
     6481 attcgctcgc gatgtggcac ctgagccagg gcgacggcgc cgaaggcgtg cgcgcgttcc
     6541 tcgagaaacg cccggctcag ttcacgtcga aagcctcgac ggacctgccc gggttcgccg
     6601 cggagtggat gaaggctccg tgatgtctgc acctctccta ctgcgtggga gaggtcaagg
     6661 atcagcgtcg acccttcgcg acctgccgat gccggtgcag cagcggctcg gtgtatccgg
     6721 agggctgttt cgttccctcg aacaccaggt cgcacgcggc ctggaaggcc gggccgtcga
     6781 acgcgggcgc catcgggcgg tagacaggat ctcccgagtt ttgcctgtcc acgaccgcgg
     6841 ccatgcgtcg catgacttcc atcacgcgct cgggcgtgac gatgcggtgg tgcagccagt
     6901 tcgcgatgtg ctgggacgag atgcggcagg ttgcacgatc ttccatcagc ccgacgttgt
     6961 tgatgtccgg caccttggag cagccgacgc cctggtcgat ccagcgcacc acatagccga
     7021 gaatgccctg cgcgttgttc tccacctcgc gccgcacctg ttccttcgac cacttgcggt
     7081 agctcgcgag cgggatgtcc agcagcccgt c
//
LOCUS       DASPFR010000221         7987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1650231, whole genome shotgun sequence.
ACCESSION   DASPFR010000221 DASPFR010000000
VERSION     DASPFR010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7987
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1650231"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..844)
                     /locus_tag="VD737_05655"
     CDS             complement(95..844)
                     /locus_tag="VD737_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HXW10077.1"
                     /translation="MPRPSHGHLACGLAACLVALSPAALAGAFSISPLRVDLSGSTKT
                     AALTVRNDDATAVVVQAEGMAWSQPDGEDALTASRELLVSPAVFTLAPGGQQLVRVAL
                     RRAPDPAEELSYRLIVQEVPQAAAPEFTGLRMALRLSVPVFVAADRAGDAALTWTAEH
                     DADGALTVTARNDGAVHARIQRFTVRSGNDTVLEQPSLAYVLPGASRRWRFPSRSGPP
                     LAPGTLPTAPYRLEALTDRGPIATELRFTQD"
     gene            complement(853..1353)
                     /locus_tag="VD737_05660"
     CDS             complement(853..1353)
                     /locus_tag="VD737_05660"
                     /inference="COORDINATES: protein motif:HMM:NF017079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HXW10078.1"
                     /translation="MRANVLVPVVAGALLITAADAGAATATTTFNVSAQVAANCLVSA
                     DDIDFGSYDGTAALTADGAVKVRCSNGLPFTIALSAGGGSFTQRLLTDGTNDLEYNLY
                     TAASFGTVWGDGTGTTVTVGDVGEGMSATKELTHTVYGQLVNSAANQDAPAGSYSDLI
                     TVTVTY"
     gene            complement(1506..4544)
                     /locus_tag="VD737_05665"
     CDS             complement(1506..4544)
                     /locus_tag="VD737_05665"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXW10079.1"
                     /translation="MVAATPGALPVHRLHDEEIERLLVTGERRAELVALFGEAGYRDL
                     STLARRAATRRGPAGPRVYVLPGLMGSRIGTRGVLLDDVLWVDLLEIAAGHLTRLGLP
                     GGQRLVALGAMWLNSLKLKLSLRIAGFDARHHPYDWRRRVSDLARELNERIEREAPHE
                     DVLLVGHSMGGVVARVALGRQATPRISRLVQLGAPNHGSFAPVLALRGVYPTVRKLAA
                     LDRRHTAEDLARIVFRTLPALHELLPDPRLAGGPNLFDAASWPEDALRPDPKMLQQAA
                     AEQATWPEVDERCLHIAGVRQDTVVGATLRRAEFEFTLSAEGDATVPLVLAVRPGQKA
                     WYVGEKHGALPNNGRVIAATVDLLRRGTTSRLAQAASRRRRLDARPLTEASLRRVAPH
                     KVRWQHLSPDARRRLLEPVVSPEFHGAVTPAALERAFAAGPPQAAALAPPRRVLEIRL
                     VHGSIVDANARAIVLGVFRNVDPSGPAGAVDAALGGAIREFTLRRMFAGQLGQVFVMP
                     AARSALLADMVLFAGLGDFDDFGAEALEFVAENVVRTFAHTHVEDFATVLFGAGSGVP
                     VAMAAEQQLRGFLAGLQHADPDRVVRRITLCELDRRKYASLVKAMRRLAVEHSGEEMQ
                     IVVDESASTAPAGPRPRAKAAPSSALRVDPAYLLVTLDASGRDGHLCRTSLLTAGAKA
                     AVLSGSQAVKRVELQRQIAPLEDGSALSRDVARIGGALARLLIVPAVREGLQSMQARP
                     LVVLHDREASRVPWEVLRVGDVHPALGAGLSRRYASEDLSVARWREDRAIDGPLRVLL
                     IVDPTGDLPGAAAEGAALQALLRERATLLDVLQGRTATRSRVLAALGAGTYDVLHFAG
                     HAFFDAATPGQGGLVCAGKEVLRGADLANLGALPSLIFCNACEAARVRKRQGRRSRSR
                     TVAQGLPGARRSMTGIAEAFLSGGVANFLGTHWPVGDEAAAAFSRALYETLLRGDTLG
                     AAVLAARRRVAAIPSIDWADYVHYGNAEFRLVTDVSFQDA"
     gene            complement(4564..5013)
                     /locus_tag="VD737_05670"
     CDS             complement(4564..5013)
                     /locus_tag="VD737_05670"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HXW10080.1"
                     /translation="MKPAFVATGLAMLALAAPVVAADLQVRDAWARATPPGAGVAAVY
                     LTIVGGDAADRLVAAKTDRAAMTQVHAVTESDGMARMREAGLVDVPPHASVVFAPNGL
                     HLMLMDLGAPLVAGQPFVVTLTFERSGTREVDVEVLAPGQAPPTARR"
     gene            complement(5010..5912)
                     /locus_tag="VD737_05675"
     CDS             complement(5010..5912)
                     /locus_tag="VD737_05675"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXW10081.1"
                     /translation="MNLRDLRYLVALADERHFGKAAERCFVSQPTLSAQVRKLEEYLG
                     VPLVERQPKRVALTPTGEKVVRRARALLQEADAIVELAKNDRDPLAGPLKLALIPTVG
                     PYLLPHVVGRLRKELPRLKLMLYEYQTEPLLEKLRAGEIDLGVLALPVPLDGLESAEL
                     YDEPFMLAMPAAHALADVERVKVDDLRGETLLLLEDGHCLRDQALEVCSRIRVSEAQD
                     YRATSLETLRQMVAAGHGITLLPELAAETPVGTARGLRIKPFSRPAPSRTIGAVWRKS
                     TTRAPAIEAIVAAVRGAMRQESKK"
     gene            5985..6407
                     /locus_tag="VD737_05680"
     CDS             5985..6407
                     /locus_tag="VD737_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXW10082.1"
                     /translation="MNARPTRLSAGVVVVRREGDDWRYLLLRAFNHWDFPKGMVEEGE
                     EPLAAAVREVKEESLIDDLDFAWGDASTQTGPYSGGKVARYYLAETRQVGVTLPFNPL
                     IGRAEHSEYRWVDYDGALELVSPRVKPVVRWAAQRLAK"
     gene            complement(6560..7558)
                     /locus_tag="VD737_05685"
     CDS             complement(6560..7558)
                     /locus_tag="VD737_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HXW10083.1"
                     /translation="MIETHAAAAPGAGLRAIFLDYGTVSCNGDLDTASLLRALPALEL
                     RTHTPQAAVGDVIADASVVMLNKLRLDRNALEGAPALKLVLLSATGTNNVDLEAARER
                     GIAVCNLRDYCTASVVQHVLGVVLLLTHKLREYDSLVRSGAWQRGDQFCRLDHPIREL
                     AGRRLGIVGYGTLGRAVARAANLALGMQVLVADRPGGARVTGRHDLDRMLPQVDVLSL
                     HCPLTPATQGLIDESRLAAMKPDALLVNTARGALVDSRALADALRAGRLGGAAIDVLS
                     QEPPVDGDPLLEPDVPNLIVTPHVAWAAREARQRCLDEMALNVEDFLRGGHRGRVV"
     gene            7727..>7987
                     /locus_tag="VD737_05690"
     CDS             7727..>7987
                     /locus_tag="VD737_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10084.1"
                     /translation="MRAERREAVLQRFDAALIEGDGVAGAHCVHELWMRGEFGLNVEA
                     LLTRLWAHASTTIPDWLPMRYVEWLPTVYDVAARFQRSRRGRH"
BASE COUNT         1146 a         2878 c         2852 g         1111 t
ORIGIN      
        1 cgcctcgacg aacggttccg ggtccgctgc cgcgcctgcc acggcggtgg cgaccagtag
       61 caggcaggca gcgatgcccg ggcgcagcgg cacgtcaatc ctgcgtgaat cgcagctcgg
      121 tcgcgatcgg gccgcggtcg gtcagggcct ccaggcggta cggagccgtc ggcagcgtgc
      181 cgggcgcgag gggggggccc gacctcgagg ggaagcgcca gcgacggctg gcccctggca
      241 gcacataggc gaggctgggc tgctccagga cggtgtcgtt gccgctgcgg acggtgaatc
      301 gctggatccg tgcgtgcacg gccccgtcgt tgcgtgccgt caccgtcagt gcgccgtccg
      361 catcgtgctc ggcggtccac gtgagcgcag cgtcgcccgc gcgatcggcg gcgacgaaca
      421 ccggcacgct gagtcgcagc gccatccgca gaccggtgaa ctcaggcgcc gcggcctgcg
      481 gcacctcctg cacgatcagc cggtacgaca actcctcggc cggatcgggc gcgcggcgca
      541 gcgcgacgcg caccagctgc tggccgccgg gcgccagcgt gaaaaccgcc ggcgacacca
      601 gcagctcgcg cgaggcggtg agcgcatcct cgccgtccgg ttgcgaccac gccatcccct
      661 cggcctgcac gaccacggcg gtggcgtcgt cattgcgcac ggtcagcgcc gccgtcttcg
      721 tcgaaccgga gaggtcgacg cgcaacggcg agatcgagaa ggcgccggca agtgcggcgg
      781 gcgacagcgc aacgaggcac gctgccagcc cgcacgcgag gtgcccgtgg ctgggccgtg
      841 gcacgtcggg cctcagtagg tgaccgtgac cgtgatcagg tcgctgtagc tgccggccgg
      901 cgcatcctgg ttcgcggcac tgttgaccag ctgcccgtag accgtgtgcg tgagctcctt
      961 cgtcgccgac atgccttcgc cgacgtcgcc caccgtgacg gtggtgcccg tgccgtcacc
     1021 ccagaccgtc ccgaagctgg cggcggtgta caggttgtac tcgaggtcgt tggtgccgtc
     1081 cgtcagcagg cgctgcgtga acgagccgcc gcccgcgctc agggcgatcg tgaagggcag
     1141 gccgttcgag cagcggacct tgaccgcgcc gtcggcagtg agcgccgcgg tgccgtcgta
     1201 gctgccgaag tcgatgtcgt ccgccgagac taggcagttg gccgccacct gcgcgctgac
     1261 gttgaacgtc gtcgtcgcgg tcgccgcgcc ggcatcggcc gccgtgatca gcagtgcacc
     1321 cgccacgacg ggtaccagta cgttcgctcg catgaatcgt cctcttcgtt gttgattggt
     1381 cgtccacgac ctcacggccg tggcgggcga ttctcgtgga gcgtggatat gtaaaacgtg
     1441 actggcgttt cgtttttcga aacgaagcca gcactggagt tcgggaaacc gccgttgcgg
     1501 cgggttcaag cgtcctggaa ggagacgtcc gtcacgagcc ggaactcggc gttgccgtag
     1561 tgcacgtagt cggcccagtc gatcgaggga atcgcggcga cccgccggcg cgcggccagc
     1621 acggccgcac cgagcgtgtc gccgcgcagc aatgtctcgt acagagcccg cgagaacgcc
     1681 gcggccgctt cgtcgcccac cggccagtgc gtgccgagga agttggcgac gccgccggac
     1741 aggaacgctt ccgcgatgcc agtcatcgag cggcgtgcgc cgggcagccc ctgcgcgacg
     1801 gtccgcgagc gagaccggcg cccctggcgc ttgcgcacgc gggcggcttc gcaggcgttg
     1861 cagaagatca gggaaggcag ggcgcccagg ttggccaggt ccgcgccgcg cagcacttcc
     1921 ttgcccgcgc agacgagacc gccctggccc ggtgtcgcgg cgtcgaagaa cgcgtgcccg
     1981 gcgaaatgca gcacgtcgta ggtgccggcg ccgagcgcgg ccagcacgcg cgagcgcgtg
     2041 gcagtacgtc cctggagcac gtcgagcagc gtcgcgcgtt cgcgcagcaa cgcctgcagg
     2101 gccgcgcctt ccgcggcggc cccgggcagg tcgccggtcg ggtccacgat gagcagcacg
     2161 cgcagcggcc cgtcgatcgc gcgatcttcg cgccaccgcg cgacgctcag gtcctcgctc
     2221 gcgtagcgac ggctgaggcc cgcgccgagc gcaggatgca cgtcgccgac gcggagcacc
     2281 tcccacggta cgcgggaggc ctcgcgatcg tgcagcacga cgagcggtcg cgcctgcatc
     2341 gactgcaggc cttcacgcac cgcgggcacg atcagcagcc gcgccagcgc tcccccgatc
     2401 cgcgcgacgt cacgcgagag cgcgctgcca tcctcgagcg gcgcgatctg gcgctgcaat
     2461 tccacccgct tcacggcctg gctgccgctc agcacggccg ccttggcccc ggccgtcagc
     2521 agcgacgtgc ggcacaggtg cccgtcgcgc ccggacgcgt cgagcgtcac gagcaggtag
     2581 gcgggatcga cacgcagggc ggagctcggc gccgccttcg cgcgtggtcg cggaccggcg
     2641 ggcgcggttg aggcggactc gtcgacgacg atctgcatct cttcgccgga gtgctcgacc
     2701 gcaaggcgcc tcatggcctt gaccagcgac gcgtacttgc gtcgatcgag ctcgcacagc
     2761 gtgatgcggc gcaccacacg gtcgggatcg gcatgctgga gtccggcgag gaatccccgc
     2821 agctgttgtt cggccgccat cgcgaccggc acgcccgaac ccgcgccgaa cagcaccgtc
     2881 gcgaagtcct ccacgtgcgt gtgcgcgaac gtgcgcacga cgttctccgc cacgaactcg
     2941 agcgcttccg cgccgaagtc gtcgaaatcg cccagtcccg cgaacagcac catgtccgcg
     3001 agcagcgcgc tgcgggccgc cggcatcacg aacacctggc cgagctgccc cgcgaacatg
     3061 cgccgcagcg tgaactcgcg gatcgcgcca ccgagtgccg cgtcgacggc gcccgccggc
     3121 ccgctcggat cgacgttgcg gaacacgccg agcacgatcg cgcgggcgtt cgcgtccacg
     3181 atgctgccgt gcaccagccg gatctcgagc actcgacgcg gcggagcgag tgcggcggcc
     3241 tggggcgggc cggccgcgaa cgcacgctcc agggccgccg gtgtcacggc cccgtggaac
     3301 tccggcgata ccacaggctc gagcagtcgc cggcgcgcat cgggtgacag gtgctgccag
     3361 cgcaccttgt gcggggcgac ccgacgcagc gacgcttcgg tcagcggccg agcgtccagc
     3421 cgtcgccggc gcgaagccgc ctgcgccagg cgcgacgtgg ttccgcggcg cagcaggtcc
     3481 accgtcgcgg cgattacacg accgttgttc ggcagcgccc cgtgcttctc gccgacgtac
     3541 cacgccttct gcccagggcg cacggcgagc accaggggaa ccgtcgcatc gccctcggcg
     3601 gacagcgtga attcgaactc ggcgcggcgc agcgtcgccc cgacgaccgt gtcctggcgt
     3661 acgcccgcga tgtgcaggca gcgctcgtcg acctccggcc acgtcgcctg ctcggccgcg
     3721 gcctgctgca gcatcttcgg gtccggccgc agcgcgtctt ccggccagga cgcggcatcg
     3781 aacaggttcg ggcccccggc caggcgcggg tcgggcaaca gttcgtgcag cgcgggcagc
     3841 gtccggaaca cgatgcgggc caggtcctcg gccgtgtgcc ggcggtccag cgccgcgagc
     3901 ttgcgtacgg tcgggtacac acctcgcagc gcgagcaccg gtgcgaacga accatggttg
     3961 ggagcaccga gttgcacgag gcgcgagatg cgcggtgtgg cctggcgacc cagggcgacg
     4021 cgggcgacga ccccgcccat gctgtggccg accaggagca cgtcctcgtg cggcgcttcg
     4081 cgctcgatgc gctcgttcag ttcgcgggcc aggtcgctga cgcggcgccg ccagtcgtac
     4141 gggtggtgac gcgcatcgaa gccggcgatg cgcagcgaaa gcttgagctt gaggctgttg
     4201 agccacatcg cgccgagcgc cacgagccgc tggccccctg gcaatccgag cctcgtcaaa
     4261 tgaccggcgg cgatctcgag caggtccacc cagaggacgt cgtcgagcag cacgcctcgc
     4321 gtgccgatcc gcgaacccat cagccccggc agcacgtaga cgcgcgggcc cgccggcccc
     4381 cgtcgcgtgg cggcgcgccg tgcgagcgtg ctcaggtcgc ggtagccggc ctcaccgaac
     4441 agcgcgacga gctcggcgcg tcgctcgccg gtgacgagca gccgctcgat ctcctcgtcg
     4501 tgcagccggt ggacgggcag ggcgccgggc gtagctgcca ccatgctgtc gtcgcccgcg
     4561 ccgtcagcgt cgcgccgtgg gcggcgcctg gccgggcgca aggacttcga cgtcgacctc
     4621 gcgcgtgccg gagcgctcga acgtcagcgt gacgacgaac ggctgcccgg ccaccagcgg
     4681 agccccgagg tccatcagca tcaggtgcag gccgttcggc gcgaatacca ccgacgcgtg
     4741 gggcggcacg tcgacgaggc ctgcttcacg catgcgtgcc atgccgtcgc tctcggtcac
     4801 ggcgtgcacc tgggtcatcg cggcacggtc ggtcttcgct gcgaccagcc ggtcggccgc
     4861 gtccccgccc acgatcgtca gatacaccgc cgcgacgccg gcgccgggcg gcgtggcgcg
     4921 cgcccacgcg tcgcgcacct gcaggtcggc tgcgacgaca ggcgctgcga gggcgagcat
     4981 cgccaggccc gtggcgacga atgctggctt cacttcttcg actcctgtcg catcgcgccg
     5041 cgcacggccg cgacgatggc ttcgatcgcc ggcgcgcgcg tggtcgactt gcgccacacc
     5101 gcgccgatgg tccgcgacgg cgccggccgc gagaacggct tgatgcgcaa cccgcgagcg
     5161 gtgccgaccg gcgtctccgc ggccagttcc ggcagcagcg tgatgccgtg gcctgcggcc
     5221 accatctggc gcagcgtctc gaggctggtg gcgcgatagt cctgtgcctc gctgaccctg
     5281 atccggctgc atacctcgag cgcctggtcg cgcaggcaat gaccgtcctc gagcagcagc
     5341 aacgtctcgc cgcgcaggtc gtccaccttg acgcgctcga cgtccgcgag cgcgtgcgcg
     5401 gccggcatgg cgagcatgaa cggctcgtcg tagagctcag ccgattcgag gccgtcgagg
     5461 ggcaccggca gtgcaaggac gcctaagtcg atctcgccgg cgcgcagttt ctcgagcagc
     5521 ggctcggtct ggtactcgta cagcatcagc ttgagccgcg gcagttcctt gcgcaggcgg
     5581 ccgacgacgt gaggcagcag gtacggcccc accgtcggga tcagtgcgag cttgagcggc
     5641 ccggcgagcg ggtcgcggtc gttcttcgcg agttcgacga tcgcgtcggc ctcctgcagc
     5701 agcgcgcgcg cgcgtcgcac gaccttctcg ccggtcggcg tcaacgccac gcgcttcggc
     5761 tggcgctcga ccagtggcac gccgaggtac tcctcgagct tgcgcacctg cgcagacaac
     5821 gtgggctggc tcacgaagca gcgctcggcg gccttgccga aatgccgctc gtcggccagg
     5881 gccacgaggt aacgcaggtc gcggagattc atgggtcgat aatagccttc gcctatcggg
     5941 aggcgcaaag caatccgacc gatgaatcgc tatacttcgc ccccatgaat gcacgcccca
     6001 cgcgcctgtc cgccggcgtg gtggtcgtcc gccgcgaggg cgacgactgg cggtacctgc
     6061 tgctgcgtgc gttcaaccac tgggacttcc ccaagggcat ggtcgaggaa ggcgaggagc
     6121 cgcttgccgc cgccgtgcgc gaggtcaagg aggagtcgct gatcgacgac ctcgacttcg
     6181 cgtggggcga cgcctccacc cagacgggtc cgtacagcgg tggcaaggtc gcccgctact
     6241 acctcgccga gacccgccag gtcggcgtga cgctgccgtt caatccgttg atcggccggg
     6301 ccgagcacag cgagtatcgc tgggtcgact acgacggggc gctcgaactc gtgtcgccgc
     6361 gcgtgaagcc cgtcgtgcgc tgggcggcgc agcgcctcgc gaagtaggcg agcgcagcgg
     6421 ccttccgggc atccctggcg gctcgcactc gccatccctg gctcgtgtcg ctccccggat
     6481 cgaaatccgg atgtccgcac caccgcgctg cagcgacctc acgcgccgct ggggcttcac
     6541 gcgccggcct gtccccaact caaacgacgc gtccgcggtg cccaccgcgc aggaagtcct
     6601 cgacgttgag cgccatctcg tccaggcacc gctgacgcgc ctcgcgcgcg gcccacgcga
     6661 cgtgcggcgt gacgatcagg ttcggcacgt cgggttcgag cagcggatca ccgtctacgg
     6721 gcggctcctg cgacagcacg tcgatggccg cgccaccgag ccggccggcg cgcagggcgt
     6781 cggccagcgc acgggagtcg accagcgcgc cgcgcgcggt gttcaccagc agcgcgtcgg
     6841 gcttcatcgc ggccagccgc gactcgtcga tcagcccctg cgtcgcgggc gtcagcggac
     6901 agtgcaggct cagcacgtcg acctgcggca gcatgcggtc gaggtcgtgg cggccggtga
     6961 cgcgggcgcc gccagggcgg tcggcgacca gcacctgcat ccccagcgca agattggccg
     7021 cgcgcgcgac ggcgcgcccg agcgtgccgt agccgacgat gcccagtcgt cggcccgcga
     7081 gttcgcggat cgggtggtcg agccggcaga actggtcgcc acgctgccag gcgccgctgc
     7141 gcaccagcga gtcgtactcc cgcagcttgt gcgtgagcag cagcacgacg ccgagcacgt
     7201 gctgcacgac cgacgccgtg cagtagtcgc gcaggttgca gaccgcgatg ccgcgttcgc
     7261 gtgctgcctc caggtcgaca ttgttcgtgc ccgtcgcgga cagcagcacg agcttcagcg
     7321 ccggcgcgcc ctcgagcgcg ttgcggtcga gccgcaactt gttgagcatg acgacggagg
     7381 cgtccgcgat cacatcaccc accgccgcct gcggcgtgtg ggtgcgcagc tccaatgccg
     7441 gcagcgcgcg aagcagcgac gcggtgtcga gatcaccgtt gcagctgacg gtgccgtagt
     7501 cgagaaagat ggcgcgcaag ccggcacctg gcgcggccgc ggcgtgggtc tcgatcatgc
     7561 agggctctcg gcggagacgg tttcgcgcta ggttagcgcg tacgcgccgc gtccgtccgc
     7621 ggccccattg cgctcctgaa cgaaaatgac gacgaggaag cccgccgccg ttcgccctgg
     7681 acgcgggcgc ggcgtcacca ggacgccgtc ccccgccgat cggccgttgc gcgccgagcg
     7741 tcgcgaggcg gtgctgcaac gcttcgacgc ggcgctgatc gaaggtgacg gggtcgccgg
     7801 cgcgcactgt gtgcacgaac tctggatgcg cggcgagttt ggcctgaacg tcgaggcgct
     7861 gctcacgagg ctgtgggcgc acgcctccac gacgatcccg gactggctgc cgatgcggta
     7921 cgtcgagtgg ctgcccacgg tctacgacgt ggcggctcgg ttccagcgct cgcgtcgcgg
     7981 gcgccac
//
LOCUS       DASPFR010000222         2231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1528266, whole genome shotgun sequence.
ACCESSION   DASPFR010000222 DASPFR010000000
VERSION     DASPFR010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528266"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1179)
                     /locus_tag="VD737_05695"
                     /pseudo
     CDS             complement(205..1179)
                     /locus_tag="VD737_05695"
                     /inference="COORDINATES: protein motif:HMM:NF008024.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
     gene            complement(1190..>2231)
                     /locus_tag="VD737_05700"
     CDS             complement(1190..>2231)
                     /locus_tag="VD737_05700"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HXW10085.1"
                     /translation="LFPENQEKLVITVAPYGPEWTPSDFPEDIPVTMEEQIQKAVDCY
                     NAGATVLHLHVRELDGKGSKRLSKFNELIAGIRARVPEMIIQVGGSISFAPESDGDVA
                     KWLGDDTRHLLAELTPRPDQVTIAVNTNQMNIVEQMCDADLAGTSIADPLLYRAYREM
                     TIPAGPEWVEEHIRRLSAKGIQTHFQLANVCQLETVERMMRRGRCNVPLVLTWVAIGG
                     GFDAPNLYNLANFVRAVPDGAVLTLETSMLNVLPINTIAIALGLHVRCGIEDNIWTQD
                     RTAKMSSVRQVEQLVRISRELGRDIADARDARRIYRIGTFFENADETLRENGFAPNRR
                     PTLRPVSKRVTA"
BASE COUNT          319 a          763 c          771 g          378 t
ORIGIN      
        1 ccttgtgtcg cagcagcagc gctttcagct cggcgaacag cgcctcgtcg cgcgcggcgt
       61 cgccgagatg gacgtcgtgg atttctgcgc cgatcgtgca ggtgagccgt tcgacgcgca
      121 gggaaccgag ggtgctgcgg cgcacgacgg cgggagcttc gagtgcctgg ctcgacatgg
      181 cgttcctcag acgacgaaga tcgacgagcc cgtggtccgg cgtgcctcga ggtcctcgtg
      241 ggcctgggcc gcgtcctcga gcgcgtagcg ctggttgatt tcgatccgga tgcgcccggc
      301 agccacgtgc ccgaagacct cggacgccag tgcggctttt tctgccgggt cggcgatgta
      361 gtccgcgagc gccgggcgcg tgacgaacag cgaccccttc acggccagct gcatggcttc
      421 gatcggcggg gtcgcacccg aagcggtgcc gacgcagacg agcaggccgc ggcgcgcaag
      481 cgagtcgatc gatccggcgt atgtgtcttt cccgacgctg tcgtagacca cgcgcacccc
      541 ggcgccattc gtgatctcgc gcacgcgtcg cgccacgtcc tcgtgccggt acaggatcac
      601 gtgatcgcag ccgcgggccc gcgcgacctc ggctttcgct tccgaggaca cggtgccgat
      661 gacgtggagg cccagcaact gggcccactg cgccacgatc aggccgacgc caccggccgc
      721 ggcgtgcagc aggatccagt cgccggggtg cagcggccac acgcgccgca gcaggtaagc
      781 ggccgtgagc ccgcgcatcg tcatcgcggc cgcggtctcg cactcgatgc ccgtgggcag
      841 cttgatcagc gcggcggcag ggatcagccg cccggtggaa tacgctccga gcgtgttgac
      901 gaagcccgtg taggtcacgc gatcgccggg cttgacgttg gtcacgccct cgcccactgc
      961 atcgacgact cccgcggcct cgactcccag gcccgacggc agcggcaccg ggtacaggcc
     1021 cgtgcggaag tacgtgtcgg caaaattgat gccgacggcc gcgtgtcgca gccgcgcctg
     1081 gcccggcccc ggctcgccga ttgcgacctc ctcgagctgg agcacctccg gcgcgccggt
     1141 gcggtgcatg cggatcgcct gcgccatgtt catgctcacg ctgcgcgcct caggccgtga
     1201 cgcgcttcga gaccgggcgc agcgtcggac ggcgattcgg cgcgaagccg ttctcgcgca
     1261 acgtctcgtc tgcgttctcg aagaacgtgc cgatccggta gatgcgtcgc gcgtcgcgtg
     1321 catccgcgat gtcgcggccg agctcgcgag agatccgcac cagctgctcc acttgccgca
     1381 ccgacgacat cttcgccgtg cggtcctgcg tccagatgtt gtcctcgatg ccgcagcgca
     1441 cgtgcaggcc gagcgcgatc gcgatcgtgt tgatcggcaa cacgttcagc atcgacgtct
     1501 cgagcgtgag cacggcgccg tcgggcacgg cccgcacgaa gttggcgagg ttgtagaggt
     1561 tgggcgcgtc gaacccgccg ccgatggcca cccacgtcag gaccagcggc acgttgcagc
     1621 gaccgcggcg catcatgcgc tccacggtct cgagctggca gacgttggcg agctggaagt
     1681 gggtctggat gcctttcgcg ctcaggcgcc ggatgtgttc ctcgacccac tccggccccg
     1741 ccggaatcgt catctcgcga taggcccgat acagcagtgg atcggcgatc gaggtgcccg
     1801 cgaggtccgc gtcgcacatc tgctcgacga tgttcatctg gttcgtgttg accgcgatcg
     1861 tgacctggtc cggtcgcggc gtgagctcgg ccagcaggtg gcgcgtgtcg tcgccgagcc
     1921 acttcgcgac gtcgccgtcg ctctccggcg caaacgagat cgatccgccg acctggatga
     1981 tcatctcggg cacgcgcgcg cggatgccgg cgatcagctc gttgaacttc gagagccgct
     2041 tgctgccctt gccgtcgagc tcgcgcacgt gcaggtgcag gacggtggcg ccggcgttgt
     2101 agcagtcgac cgccttctgg atctgctctt ccatcgtcac cgggatgtct tcggggaagt
     2161 cggagggcgt ccactcgggc ccgtacggcg cgacggtgat gacgagtttt tcctggttct
     2221 ctgggaacag c
//
LOCUS       DASPFR010000223         4636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1039519, whole genome shotgun sequence.
ACCESSION   DASPFR010000223 DASPFR010000000
VERSION     DASPFR010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039519"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            155..1507
                     /gene="hslU"
                     /locus_tag="VD737_05705"
     CDS             155..1507
                     /gene="hslU"
                     /locus_tag="VD737_05705"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HXW10086.1"
                     /translation="MTPREIVQELDKHIVGQASAKRAVAIALRNRWRRMQVAEPLRAE
                     ITPKNILMIGPTGVGKTEIARRLARLANAPFVKVEATKFTEVGYVGREVDSIVKDLVD
                     VAVKMTREQEMEKVRHRAMDAAEERVLDVLLPRPRTTDPTVGFGLRQEEPAPTPRDND
                     TRQRFRKMLREGELDEREIEVELRAVSSNVEIMAPPGMEELSQQLQGMFSSLGGGRTR
                     SRRLKVREAFKLLTEEEAAKLINEDELKAQALRNAEQNGIVFIDEIDKIARQQGSVSG
                     VDVSREGVQRDLLPLVEGSTVSTKYGMVKTDHVLFIASGAFHMSKPSDLIPELQGRLP
                     IRVELEALKVEDFVRILTEPDASLCSQYTALLETEGVRLRFDEGGVRRIAEVAFDVNQ
                     RTENIGARRLHTVMERLLEAISYEASDRSGSELVVDGGYVDASLGELARDEDLARYIL
                     "
     gene            1504..1920
                     /locus_tag="VD737_05710"
     CDS             1504..1920
                     /locus_tag="VD737_05710"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HXW10087.1"
                     /translation="MTADKGATYWAERKPLEIKLRRQSRLLEIAFDDGRRFELPFEYL
                     RVYSPSAEVKGHGPGQETLQLGKHDVQINSIDPVGHYAVRLVFDDGHDSGLYAWGYLY
                     ELGATHAEKWEHYLERVAKLGLPYPTPARGKPIKLK"
     gene            2032..2826
                     /gene="ubiE"
                     /locus_tag="VD737_05715"
     CDS             2032..2826
                     /gene="ubiE"
                     /locus_tag="VD737_05715"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008080626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HXW10088.1"
                     /translation="MSAPKSRTPPAPPADPPPSAPVDFGYEQVPAHEKARRVREVFDS
                     VAGKYDVMNDLMSAGVHRLWKRYTLGQTGLRPGQSALDVAGGTGDLAAGMAAQVGDAG
                     LVVLSDINAAMLEVGRDRLLDRGLLRNVRYSLADAECLPFADETFDCVTIGFGLRNVT
                     DKAAALASMRRVLRPGGRLLILEFSKPTFPGLKPVYDLYSFSVLPWLGARIARDADSY
                     RYLAESIRRFPDQETLLGMMREAGLEDCRYENLSGGIVALHRGFRY"
     gene            2823..3449
                     /locus_tag="VD737_05720"
     CDS             2823..3449
                     /locus_tag="VD737_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HXW10089.1"
                     /translation="MSANPLNALLRQLAASLNRNVGQSERAATLAAQLDGRVLALVLE
                     GTPMTLWFKVNGGQVAIDTRHEGAADARLAGTPLSLLSLVGPGAPERVRSAGIRIEGD
                     AEVAQRFQGLLQLAQPDFEEELARAIGDVAAHQVANFARGLLDWGRKAADSFSQNVAE
                     YLQEEGRDVPSRVELEEFLEAVDHVRNYADRLEARLSRLESRRRGGFK"
     gene            3510..>4636
                     /locus_tag="VD737_05725"
     CDS             3510..>4636
                     /locus_tag="VD737_05725"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HXW10090.1"
                     /translation="MRLRVATRLLDIQRVLVRHGLDEFILATHLFRPLRFAFYLSPAT
                     WFERRKGGPRGERIRLALEELGPVFVKFGQALSTRRDLLPPDIADELAKLQDRVPPFP
                     TEQAKAIIARAYGHPAGEVFERFDDAPLAAASIAQVHSARLRSGEEVVVKVVRPGVRE
                     RIERDLEVMYVLAQLAHDYSREGYRLRPLEVVAEYERTIIDELDLMREAANAAQLKRN
                     FAGSDLLYVPQVHWDYCHPDVMVMERIHGVLVNDMDELRQRGVNIRVLAENGVEIFFT
                     QVFRHNFFHADMHPGNIFVQVHDPERPVYAAVDFGIVGTLDARDQHYLAENFLAFFDR
                     DYNRVARLHIDSGWVPAETRVDELESAVRTVCEPIFYKPLE"
BASE COUNT          760 a         1588 c         1584 g          704 t
ORIGIN      
        1 cgtaatatga ccatcgagga actgtagaga aagtgtccag tgttcagtgt ctagcggtta
       61 gtcagagatg gatgttcgac gcagtcctcc tactaaccgc tggacactag acactagaca
      121 ctagacactc atctcgctga ccgccatgtc atcgatgacg cccagagaga tcgtccagga
      181 actggacaag cacatcgtcg gccaggcgtc ggcgaaacgc gcggtcgcga ttgcgctgcg
      241 caaccgctgg cgccgcatgc aggtggccga gccgttgcgc gccgagatca cgccgaagaa
      301 catcctgatg atagggccga ccggggtcgg caagaccgag atcgcgcgcc gcctcgcgcg
      361 cctcgccaac gcgccgttcg tgaaggtcga ggcgaccaag ttcacggagg tcggctacgt
      421 cggtcgcgag gtggactcga tcgtcaagga cctcgtcgac gtcgcggtca agatgacgcg
      481 cgagcaggaa atggaaaagg tgcgccatcg tgcgatggac gcggccgagg agcgcgtgct
      541 cgacgtactg ctgccgcgcc cccgcacgac ggacccgacg gtcggcttcg gcctgcgaca
      601 ggaggagccg gcgccgacgc cgcgggacaa cgacacgcgc cagcgctttc gcaagatgct
      661 gcgcgagggc gagctcgacg agcgcgagat cgaagtggag ctgcgtgcgg tctcgtccaa
      721 tgtggagatc atggcgccgc ccggcatgga ggagctctcg caacaactgc agggaatgtt
      781 ctcgagcctc ggcggcggcc gcacccggtc gcgtcgtctg aaggtccgcg aggcgttcaa
      841 gctgctgacc gaggaagagg cggccaagct gatcaacgag gacgagctca aggcgcaggc
      901 gctgcgcaac gccgagcaga acggcatcgt cttcatcgac gagatcgaca agatcgcgcg
      961 ccagcagggc tcggtgtcgg gcgtcgacgt ttcgcgcgag ggcgtgcagc gcgacctgct
     1021 gccgctcgtc gagggcagca cggtcagcac caagtacggc atggtcaaga ccgaccacgt
     1081 gctgttcatc gcctcgggcg ccttccacat gtcgaagccg tcggacctga tcccggagct
     1141 gcaggggcgg ctgccgatcc gcgttgagct cgaagccctc aaggtcgagg atttcgtgag
     1201 gatcctgacc gagccggacg cctcgctgtg ctcgcaatac accgcgctgc tggagaccga
     1261 gggcgtgcgg ctgaggttcg acgagggcgg cgtccggcgc atcgccgagg tcgcgttcga
     1321 cgtcaaccag cgcaccgaga acatcggcgc gcgtcggctg cacaccgtga tggagcggct
     1381 gctcgaggcg atcagctacg aggcctcgga ccgttccggg tcggagctcg tcgtcgacgg
     1441 cggctacgtg gacgcgagcc tcggcgagct ggcccgcgac gaggacctgg cgaggtacat
     1501 cctgtgactg cagacaaggg cgcgacgtac tgggccgagc gcaagccgct cgagatcaag
     1561 ttgcggcgcc agtcccgctt gctggagatc gccttcgacg acggtcggcg cttcgagctg
     1621 ccgttcgaat acctgcgcgt gtactcgccc tcggccgagg tcaagggcca cggacccggg
     1681 caggagacgt tgcagctcgg caagcacgac gtccagatca actcgatcga tccggtcggc
     1741 cactacgccg tgcggctggt gttcgacgac ggccacgatt ccggcctgta cgcgtggggc
     1801 tacctttacg aactcggcgc gacgcacgcc gagaagtggg agcactacct ggagcgtgtc
     1861 gcgaagctgg ggctgccgta cccgacgccg gcgcgcggca agccgatcaa gctcaagtag
     1921 ctgcgctccc gattcgccgc ccgcgggcgc ctcccggccc ggcggccgcg gtgccccgtc
     1981 ggtggttgcc gcagcggccg ccaattcgaa ccgcgtagaa tgggcgttcc catgagcgcg
     2041 cccaagtccc gaaccccccc ggcccctccg gccgaccccc cgccgtcggc gcccgtcgac
     2101 ttcggctacg agcaggtgcc cgcgcacgag aaggcacgcc gcgtacggga ggtgttcgac
     2161 tctgtcgccg gcaagtacga cgtcatgaac gacctgatgt cggccggcgt gcaccggctc
     2221 tggaagcgct acacgctggg ccagaccggg ctgcgcccgg gacagtcggc gctcgacgtc
     2281 gcgggcggca cgggcgacct ggcggcgggg atggccgcac aggtcggcga cgccggcctc
     2341 gtcgtgctgt ccgacatcaa cgcggcgatg ctcgaggtcg gccgcgaccg gctgctggat
     2401 cgcgggttgt tgcgcaacgt gcggtactcg ctcgcggacg cggagtgcct gcccttcgcc
     2461 gacgagacgt tcgactgcgt cacgatcggt ttcgggctgc gcaacgtcac cgacaaggcg
     2521 gccgcgctcg cgtctatgcg gcgcgtgctc aggccgggcg gacgcctgct gatcctcgag
     2581 ttttcgaagc ctacgttccc cggcctgaag ccggtctacg acctctactc gttcagcgtg
     2641 ctgccgtggc tgggcgcacg catcgcgcgc gacgccgaca gctaccggta cctcgccgag
     2701 tcgatccggc gctttcccga ccaggagacc ctgctcggca tgatgcgcga agcgggcctc
     2761 gaggactgcc gctacgagaa cctgagcggc ggcatcgtcg cgctgcaccg ggggttccgg
     2821 tattgagcgc caatccgctc aacgcactgc tacggcagct cgcggcgtcg ctgaaccgca
     2881 acgtcgggca gtccgagcgg gccgcgacgc tcgccgccca gctcgacggc cgcgtgctcg
     2941 cgctcgtgct cgagggcacg ccgatgacgc tgtggttcaa ggtgaacggc gggcaggtcg
     3001 cgatcgacac gcgacacgag ggcgccgccg acgcgcgcct ggccggtacg ccgttgagcc
     3061 tgttgtcgct cgtggggccg ggcgctcccg agcgcgtccg cagcgccggc atccgcatcg
     3121 aaggcgacgc cgaggttgcg caacgcttcc agggcctgct gcagctcgcg cagccggact
     3181 tcgaggagga gctggcgcgc gcgatcgggg acgtcgccgc gcaccaggtc gcgaacttcg
     3241 cgcgcggcct gctcgactgg ggccgcaagg ccgccgactc gttctcgcag aacgtcgcgg
     3301 agtacctgca ggaggaaggc cgcgacgtcc cgtcgcgcgt ggagctcgag gaattcctgg
     3361 aagccgtcga ccacgtgcgc aactacgcag accggctcga ggcgcgactc tcgcgcctcg
     3421 aatcccgccg ccggggcggc ttcaagtgac gctccgcgcg caccgccttc taccaaccgc
     3481 taaccgctaa ccgctagaca cttccctcca tgcgtctgcg cgtcgccacc cggctcctcg
     3541 acatccagcg tgtgctcgtc cggcacggcc tcgacgagtt catactcgcg acccacctgt
     3601 tccggccgtt gcggttcgcg ttctacctct cgcctgcgac gtggttcgag cggaggaagg
     3661 gcggcccgcg gggcgagcgc atccggctcg cgctcgagga gctcggcccg gtcttcgtga
     3721 agttcgggca ggcactgtcg acgcgccgcg acctgctgcc gccggacatc gcggacgaac
     3781 tcgccaagct gcaggaccgc gtcccgccat ttcccaccga acaggcgaag gcgatcatcg
     3841 cgcgtgcgta cggtcacccg gccggagagg tgttcgagcg cttcgacgac gcgccgctcg
     3901 cggcagcctc gatcgcccag gtgcacagcg cgcggctgcg ctcgggcgag gaagtcgtcg
     3961 tcaaggtcgt gcgtcccggc gtgcgcgaac ggatcgagcg tgacctcgag gtcatgtacg
     4021 tgctcgccca gctcgcgcac gattattccc gcgaaggcta tcggctgcgc ccgctcgagg
     4081 tcgttgccga gtacgagcgg acgatcatcg acgagctcga cctgatgcgc gaggcggcca
     4141 acgccgcgca actcaagcgc aacttcgctg gttccgacct gctctacgtg ccgcaggtgc
     4201 actgggacta ctgccacccc gacgtgatgg tgatggagcg catccacggc gtcctggtca
     4261 acgacatgga cgagctgcgc cagcgcggcg tcaacatccg cgtgctcgcc gagaacggcg
     4321 tcgagatctt cttcactcag gtgttccggc acaatttctt ccatgccgac atgcaccccg
     4381 gcaacatctt cgtgcaggtg cacgatcccg agcgcccggt gtacgccgcc gtggacttcg
     4441 gcatcgtcgg cacgctcgac gcgcgcgacc agcactacct cgcagagaac ttcctggcgt
     4501 tcttcgaccg cgactacaac cgggtcgcgc gcctgcacat cgactccggc tgggtgccgg
     4561 ccgagacgcg ggtcgacgaa ctggagagcg cggtccgcac cgtctgcgag ccgatcttct
     4621 acaagccgct cgagga
//
LOCUS       DASPFR010000224         4231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_184708, whole genome shotgun sequence.
ACCESSION   DASPFR010000224 DASPFR010000000
VERSION     DASPFR010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184708"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1627
                     /locus_tag="VD737_05730"
     CDS             176..1627
                     /locus_tag="VD737_05730"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF006943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-methylcitrate
                     dehydratase/aconitate hydratase"
                     /protein_id="HXW10091.1"
                     /translation="MSDVPTKSALRPAPDRLLVEIADYAMNYDGGSPLARETAYHCLL
                     DSLACALQALKYPACTKLLGPVVPGATMARGARVPGTSYELDPVQAAFNLGTMIRWLD
                     FNDTWLAAEWGHPSDNLGAILTVADFQSRSALAEARTPPTVRDVLRRMIQAHEIQGVL
                     ALENAFNRVGLDHVLLVRIASTAVVTAMLGGTREQVVNALSNAFLDGGALRAYRHAPN
                     TGSRKSWAAGDATSRAVRLSLMAMTGEMGYPSALTAPGWGFQDVLFRGQPLSLPQPLG
                     CYVMENVLFKISYPAEFHAQTAVECALALYPTVHDRLDDIERIVIETQESGVRILDKV
                     GPLANPADRDHCLQYIVAVPLMFGRLSAADYEDSVARDPRIDALRAKMVVRENASFSE
                     EYLAADKRHIANAIQVFFADGTASDRIQVDYPVGHRKRRAEGMPLLWQKFESSVTAHY
                     PPVQAERIRSTLADRVRVEAMPTNELVSMLVRN"
     gene            complement(1635..2042)
                     /locus_tag="VD737_05735"
     CDS             complement(1635..2042)
                     /locus_tag="VD737_05735"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HXW10092.1"
                     /translation="MRDRSTFSRLAKSAAQIAGRPKAFGTAVTLILVWAITGPLFGFS
                     DTWQLVVNTGTTIVTFLMVFLIQNTQNRDTEAMQIKLDELIRATANANNELLNLEELD
                     QSKLDAYRQKYLALAAEARDSVKRGRPDTTPET"
     gene            complement(2075..2584)
                     /locus_tag="VD737_05740"
     CDS             complement(2075..2584)
                     /locus_tag="VD737_05740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004647503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HXW10093.1"
                     /translation="MGDVEIRNERPGDEDAIHEVTTAAFLEAPHTAHTEQFIVRALRD
                     AGALTVSLVAELDRRIVGHVAISPVTIDGAACGWFGLGPVSVLPRHQGVGLGSRLVRD
                     ALARLRTAGARGCVLVGEPAYYGRFGFRAEPTLVLPGVPPQYFQALSFVADHPRGVVA
                     FHAAFAATT"
     gene            2629..3255
                     /gene="rsmG"
                     /locus_tag="VD737_05745"
     CDS             2629..3255
                     /gene="rsmG"
                     /locus_tag="VD737_05745"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HXW10094.1"
                     /translation="MTQLLDGARAMGVELDPQQAATLLAHLDLVDEWGARMNLTAIRE
                     RPQQLTKHVLDSLSVASWLRGTRVADVGSGAGFPGIPLAVARPELHFVLVESTGKKCR
                     FLEHVRDTLGLSNVEIVQSRAEAYKPAARFDTVLARAVGPIAELVRNAGALVTGGGRI
                     LAMKGRYPSDELERKLNGWKVVAVHALHVPGLAEERHLVELAHSHEKA"
     gene            3338..>4231
                     /locus_tag="VD737_05750"
     CDS             3338..>4231
                     /locus_tag="VD737_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HXW10095.1"
                     /translation="MKRVIAVANQKGGVGKTTTAVNLAASLAATQRKVLLLDCDPQGN
                     ATMGCGVDKRNVERSMTDVLLGDAELAAALVPVDPGHFTLGPGNQDLVAAEVQLMSMI
                     GREMRLRNALKTVRDQYDVVLIDCPPSLNLLTLNALVAADSVLVPMQCEYYALEGLSS
                     LMNTIEQIRASVNADLELEGILRTMYDPRNNLSNEVSGQLLMHFGDKVFRTVIPRNVR
                     LAEAPSFGKAVLYHDRDSRGALAYLALAGEMIRREEELAAGPATTESSEPVESSQPIE
                     SSQGMESAHAQEPAAPQPPVAS"
BASE COUNT          687 a         1443 c         1432 g          669 t
ORIGIN      
        1 ctcgcacgcg ccgcgctctg gcccgcggtg acggcggtga cgcacccggc gtgggcctgg
       61 acggcggccg ccctgctgct gtggctggcg gttgaggtga cggtgcacat gagccgcgcg
      121 agaacggcga ggtagacttt cggacgttcg acggggcggc catcatgttc gctgcatgag
      181 cgacgtcccc accaagtccg ccctccgtcc cgcccccgac cggctgctcg tcgagatcgc
      241 cgactatgcg atgaattacg acggcggctc gcccctcgcg cgcgagacgg cctaccactg
      301 cctgctcgac tcgctcgcct gcgcgctgca ggcgctgaag tacccggcct gcacgaaact
      361 gctcgggccg gtcgtgccgg gcgcaacgat ggcccgcggt gcccgcgtgc ccggcacctc
      421 gtacgagctc gacccggtgc aggccgcctt caacctgggc acgatgatcc gctggctcga
      481 cttcaacgac acctggctcg cggcggagtg gggccacccg tccgacaacc tcggcgcgat
      541 cctcaccgtc gccgacttcc agtcgcgcag cgcgctggcc gaagcgcgca cgccgccgac
      601 cgtgcgtgac gtgctacgcc ggatgatcca ggcgcacgaa atccagggcg tgctcgcctt
      661 agagaacgcc ttcaaccgcg tggggctcga tcacgtgctg ctcgtgcgca tcgcctccac
      721 ggccgtcgtc accgcgatgc tcggcggcac gcgcgagcag gtcgtcaacg cgctgtcgaa
      781 tgcgttcctg gacggcggtg cgctgcgcgc gtaccggcac gcgcccaaca ccggttcccg
      841 caagagctgg gccgccggcg acgcgacgag tcgcgccgtg cggttgtcgt tgatggcgat
      901 gacgggcgag atgggctacc cgtccgcgct gacggcgccg ggctggggct tccaggacgt
      961 gctgttccgc ggccagccgt tgtcgttgcc gcagccgctg ggttgctacg tcatggaaaa
     1021 cgtgctgttc aagatttcgt atccagctga attccacgca cagacggccg tcgaatgcgc
     1081 gctcgcgctg tacccgaccg tgcacgaccg gctcgacgac atcgagcgca tcgtcatcga
     1141 gacgcaggag tcgggcgtgc gcatcctcga caaggtggga ccgctcgcga accccgcgga
     1201 ccgtgaccac tgcctgcagt acatcgtcgc cgtgccgttg atgttcggtc ggctcagcgc
     1261 cgccgactac gaggactccg tcgcgcgcga tcctcgcatc gacgcgttgc gggcgaagat
     1321 ggtggttcgc gaaaacgcct cgttcagcga ggaatacctg gccgccgaca agcgtcacat
     1381 cgcaaacgcg atacaggtgt tcttcgccga cggcaccgcg agcgatcgga tccaggtcga
     1441 ctatcccgtc ggccatcgca agcgtcgcgc tgaggggatg ccactgctgt ggcaaaaatt
     1501 cgagtccagc gtgacggccc attacccgcc cgtgcaggcg gaaaggatcc gcagcacgtt
     1561 ggcagaccgc gtccgcgtcg aagcgatgcc gaccaacgaa ctcgtgtcga tgctcgtgcg
     1621 caactagcgc gccgctaagt ctccggggtc gtatcggggc ggccgcgctt cacggagtca
     1681 cgggcttccg cggcgagcgc caggtacttc tgccggtacg cgtcgagctt cgactggtcg
     1741 agctcctcca ggttcagcag ctcgttgttc gcattggcgg tcgcacggat cagctcgtcc
     1801 agcttgatct gcatcgcttc ggtgtcgcgg ttctgggtgt tctggatcag gaacaccatc
     1861 aggaaggtca cgatcgtcgt cccggtgttc acgaccagtt gccacgtgtc gctgaatccg
     1921 aagagcgggc ccgtgatcgc ccaaaccaag atcagcgtca cggccgtgcc gaaggccttg
     1981 ggacgtccgg cgatttgtgc cgcggacttt gcgagtcggg agaacgttga tcggtcgcgc
     2041 atcgggagtc ctctggtggt gccaatgcag gtgatcaggt cgtggctgca aacgccgcgt
     2101 gaaacgccac gacgccgcgg ggatgatcgg cgacgaagct caacgcctgg aaatactggg
     2161 gcggcacgcc cggcaggacg agcgtcggct cggcgcgaaa gccaaaccgg ccgtaatagg
     2221 cgggctcgcc gacgaggacg cagccccgtg cgcccgccgt tcgcaaccgc gccaaggcgt
     2281 cgcgcaccag tcgcgagccg agtcccaccc cctggtgccg cggaagcacc gagactgggc
     2341 cgagtccgaa ccagccgcac gcggcaccgt cgatcgtgac gggcgagatc gcgacgtggc
     2401 cgacgatgcg acggtcgagt tctgccacga gcgacaccgt cagcgcaccg gcgtcgcgga
     2461 gggcgcgcac gatgaactgt tcggtgtgtg cagtgtgcgg cgcctcgagg aacgctgcgg
     2521 tggtgacctc gtgaatcgcg tcctcgtcac caggacgctc gttgcggatc tccacatcgc
     2581 ccatgcagcg ctcccggttc cgctggcccg aagcgttatc ctagcccgat gacgcaactt
     2641 ctcgacggcg cccgcgcgat gggcgtcgaa ctcgacccgc agcaggcggc gacgctgctc
     2701 gcccacctcg acctcgtcga tgagtggggc gcgcgcatga acctgacggc gatccgcgag
     2761 cgcccgcagc agttgaccaa gcacgtgctg gacagcctct ccgtggcgtc gtggctgcgc
     2821 gggacgcgcg tggccgacgt cggaagcggt gccggctttc cgggcatccc gctcgccgtc
     2881 gcacggcctg agctgcattt cgtcctggtc gaatcgactg gcaagaagtg ccggttcctc
     2941 gagcacgtcc gggacacgtt ggggctttcc aacgtggaga tcgtccagtc tcgcgccgag
     3001 gcgtacaagc cggccgcccg attcgacacg gtgctcgcac gggccgtggg cccgatcgcg
     3061 gaactcgtgc gcaatgccgg agcgctcgtc accggcgggg gccggatcct ggcgatgaaa
     3121 ggccgttatc cgtcggacga gctcgagcgc aagctcaacg gctggaaagt ggtcgcggtg
     3181 cacgcactgc acgtgcctgg cctcgccgag gagcgacacc tggtcgagct cgcgcattcg
     3241 cacgagaagg cctgacacga tcgcgccgcc gccgctccga ctgaccgctg gacactaccc
     3301 gctaaccgct tcctccctgc ggtatccttg ccgttccatg aaacgagtca tcgcggtcgc
     3361 gaatcagaag ggcggcgtcg gcaagacgac caccgcggtg aaccttgccg cttcgctggc
     3421 cgcgacgcag cgcaaggtgc tgctgctcga ctgcgacccg caaggcaacg cgaccatggg
     3481 ttgcggcgtc gacaagcgca acgtcgagcg ctcgatgacg gacgtcctgc tcggcgatgc
     3541 ggagctggca gcggcgttgg tccccgtcga tccggggcat ttcacgctcg gtcccggcaa
     3601 ccaggacctc gtcgcggccg aagtgcagct gatgtcgatg atcggtcgcg aaatgcgact
     3661 gcgcaacgcg ctgaagaccg tgcgggacca gtacgacgtg gtgctgatcg actgcccgcc
     3721 gtcgctgaac ctgctgacgc tcaacgcgct cgtcgccgcg gacagcgtgc tcgtgccgat
     3781 gcagtgcgag tactacgcgc tcgaggggct gtcgtcgctc atgaacacca tcgagcagat
     3841 tcgcgcgagc gtcaacgccg acctcgagct cgagggcatc ctgcgcacga tgtacgaccc
     3901 ccggaacaac ctctcgaacg aggtctcggg gcagctgctc atgcatttcg gcgacaaggt
     3961 cttccgcacc gtgattccga gaaacgtgcg tctcgccgag gccccgagct tcggcaaggc
     4021 cgtgctgtat cacgaccggg attcgcgggg cgcgctcgcg tacctggccc tggcgggcga
     4081 gatgatccga cgcgaggaag agctcgcggc gggcccggcg accaccgagt cgagcgagcc
     4141 ggtcgagtcg agccagccga tcgagtcgag ccaggggatg gagtcggccc acgcgcagga
     4201 accggccgcc ccgcagccgc cggtggcgag t
//
LOCUS       DASPFR010000225         4522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1651026, whole genome shotgun sequence.
ACCESSION   DASPFR010000225 DASPFR010000000
VERSION     DASPFR010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651026"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VD737_05755"
     CDS             complement(<1..472)
                     /locus_tag="VD737_05755"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXW10096.1"
                     /translation="MTIKVGDRMPAGTLKTMTKDGPQNITTDQLFSGKKVVLFSVPGA
                     FTPTCDAKHLPGFVEKAGDLKAKGVDTVACMAVNDVFVMNAWGKHSGVEDKVMMLADG
                     NGEYAKALGLDFDAKGFGMGVRGKRFAVVVDDGVAKQVLIEEPGEFKVSSADYVL"
     gene            668..1225
                     /locus_tag="VD737_05760"
     CDS             668..1225
                     /locus_tag="VD737_05760"
                     /inference="COORDINATES: protein motif:HMM:NF021360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HXW10097.1"
                     /translation="MTGPSLREQLLQAGLVTEKQVKQAEQHKQRQKFNDRRQVSRKDL
                     HQREAQRLAEQERVAAAKAAKDAELNRKREEKAAAKARWAEIRQLVAQHRIAKPESDD
                     YYNFVINNKVGRVAVDAALRERIVRGEVGIVRCDGRYELLPADAVARVREREPRAVVP
                     PPAEQPTTPVDDAYKDFVVPDDLMW"
     gene            complement(1333..2415)
                     /locus_tag="VD737_05765"
     CDS             complement(1333..2415)
                     /locus_tag="VD737_05765"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF009395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXW10098.1"
                     /translation="MTERLFDINVVSSELLPTPETIKRDLAMPAAAEEFVYRSRHAVR
                     RILDREDPRLFVVVGPCSIHDPQAAREYAERLSALAQRVERTMLLVMRVYFEKPRTTV
                     GWKGLINDPDMDDSFHIEKGIRIARELLLHLAGIGVPAATEALDPIMPQYISELITWT
                     AIGARTTESQTHREMASGLSTPVGFKNGTDGSLSVAINALQSVRHPHHFLGITQQGQS
                     AVFRTRGNRYGHIVLRGGGARTNYDSVSLALCERELEKAGLPSNIVVDCSHGNSNKDP
                     GLQPLVAENLANQILEGNRSLVGFMLESNLHWGSQPLCADRSQLQYGVSVTDACIDWA
                     TTEGLLASLDERLRTAPRFARSAVAA"
     gene            complement(2468..3346)
                     /gene="kynA"
                     /locus_tag="VD737_05770"
     CDS             complement(2468..3346)
                     /gene="kynA"
                     /locus_tag="VD737_05770"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR03036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HXW10099.1"
                     /translation="MSTPDDPKGPRRPALGSHVGVEGEDIHWDLGESLSYGQYLQLDK
                     VLGAQSPLSTHHDEMLFIVMHQATELWMKLCIHELRAAVEQIRHDDLGPAFKMLARVS
                     RVQVQLAQSWDVLSTMTPADYSAFRDSLGKSSGFQSYQYRTLEYIIGNKNPAMIEVHR
                     HLPELYAQLKEALYSPSLYDESLRLLARRGFAIPAHYVERDWSVPYVESPDVTAAWLE
                     VYRNVEQHWDLYELAEKLVDLDQRFQLWRFAHLKTVERIIGYKRGTGGSSGVSYLARA
                     LDLRFFPELWNVRTSI"
     gene            complement(3343..4119)
                     /locus_tag="VD737_05775"
     CDS             complement(3343..4119)
                     /locus_tag="VD737_05775"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HXW10100.1"
                     /translation="MSSLGRGRAGTAPGPGQPMQLSLWSDREVGTAWNVRISRRARRM
                     SMRVFPGGSVEVVVPPGAGVPAIERFVARHRDWAERRSRELLRLAPKAAERQPAQVEM
                     ELLGRAWQVEYLPGRRVRAEEVGDDRLRLHVPSVSDRHASQALVPWLTRLATRELRLR
                     LDALSREIGIDFARLSVRRQRTRWGSCSTRGTISLNVCLMFQRPDVVRYLMIHELCHR
                     RHMNHSKRFWSLVASFEPDWKPLDVELLQGWRNVPAWVFG"
     gene            complement(4133..>4522)
                     /locus_tag="VD737_05780"
     CDS             complement(4133..>4522)
                     /locus_tag="VD737_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXW10101.1"
                     /translation="LAVLMGIGAVFAALNTMYSAVSSRTREIATLRALGFGAGPVVTS
                     VLLESAVLGLAGGAVGGLLVYLVLNGYQASTLNWGSFSQLTFAFTVTPSLLVTGLGYS
                     LILGLVGGLLPGIRAARMPVTTGLREL"
BASE COUNT          752 a         1526 c         1531 g          713 t
ORIGIN      
        1 cgagcacgta gtccgcgctc gacaccttga actcgccggg ctcctcgatc agcacctgct
       61 tcgcgacgcc gtcgtcgacg accactgcaa agcgcttgcc gcgcacgccc atgccgaagc
      121 ccttcgcatc gaagtcgaga cccagcgcct tggcgtactc gccgttgccg tcggcgagca
      181 tcatgacctt gtcctcgacg ccggagtgct tgccccaggc gttcatgacg aagacgtcgt
      241 tgacggccat gcaggcgact gtatcgacgc ctttggcctt gaggtcgccg gctttctcga
      301 cgaaccccgg aaggtgcttg gcatcgcagg tcggcgtaaa cgctccgggt acggagaaca
      361 gcacgacctt cttgccggag aacagctggt ccgtggtgat gttctgcggg ccgtccttgg
      421 tcatcgtctt gagcgtgccg gcgggcatcc tgtcgcccac tttgatcgtc atgatcgggt
      481 tcctccggta gtgcgtgatg aaaaacgagg tcttgctgca tcttaacagc acgctccgac
      541 gatcgcgccc gcccacggga cgtcgtcgga cgcaggcacc gacgcgcggg cggtcgtccg
      601 cttcgcatcg gcgggcagcg tgcgtaacat agcgctccct tacggacgga cgtcgagagg
      661 tcgaggcatg acgggcccgt cgttgcgcga gcagttgctg caggccggcc tggtgaccga
      721 aaaacaggtc aagcaggccg agcagcacaa gcagcgccag aagttcaacg accgtcgcca
      781 ggtcagccgc aaggacctgc accagcgcga agcgcagcgt ctcgccgagc aggagcgcgt
      841 cgcggcggcc aaggccgcga aagacgccga actcaaccgc aagcgcgagg aaaaggcggc
      901 cgcgaaggct cgctgggccg agatccgcca gctcgtcgcg cagcaccgca ttgcgaagcc
      961 ggagagcgac gattactaca acttcgtgat caacaacaag gtgggccgcg tggcggtcga
     1021 cgcggcgctc cgggagcgca tcgtcagggg cgaggtcggc atcgtgcgct gcgacggccg
     1081 ctacgaactg ctcccggcgg acgcggtcgc gcgggtgcgc gaacgcgaac cgagggccgt
     1141 ggtgccgccg ccggccgagc agcccacgac gccggtggac gacgcctaca aggacttcgt
     1201 cgtcccggac gatctcatgt ggtgaccgta aatagggaca ctcctgattt cctcccgtct
     1261 cacacgggtc cgaaaatcag gagtgtcccc atttgaattt aattcaggag tgtccccatt
     1321 ttcgggcgcc gttcaggcgg cgacggcact cctggcgaag cgtggcgccg tgcgcaggcg
     1381 ctcgtcgagc gacgccagca ggccctcggt cgtcgcccag tcgatgcacg cgtcggtgac
     1441 cgacacgccg tactgaagct ggctgcggtc ggcgcagagc ggctggctgc cccagtgcag
     1501 gttgctctcc agcatgaaac cgaccagcga ccggttgccc tcgaggatct ggttcgcgag
     1561 attctcggcc acgagcggct gcagcccggg gtccttgttc gagttgccgt gactgcagtc
     1621 gacgacgatg ttcgagggca gccccgcctt ctccagctcg cgttcgcaca gcgcgaggct
     1681 caccgaatcg tagttcgtgc gggcgccgcc gccgcgcagg acgatgtggc cgtagcggtt
     1741 gccgcgcgtc cggaacaccg ccgactgccc ctgctgcgtg atgccgagga agtggtgcgg
     1801 atggcgcacc gattgcagcg cgttgatcgc caccgacagc gaaccgtcgg tgccgttctt
     1861 gaagccgacc ggcgtcgaca ggccgctcgc catttcgcgg tgggtctgcg actcggtcgt
     1921 gcgcgcgccg atggccgtcc acgtgatcag ttccgagatg tactgcggca tgatcgggtc
     1981 gagcgcctcg gtggctgccg gcacgccgat cccggccagg tgcagcagca gctcgcgcgc
     2041 aatgcggatg cccttctcga tatggaagga gtcgtccatg tcggggtcgt tgatcagccc
     2101 cttccagccg actgtcgtgc gtggcttctc gaagtagacg cgcatgacga gcagcatcgt
     2161 gcgctccacg cgctgcgcga gtgcgctcaa ccgctcggcg tattcgcggg ccgcctgcgg
     2221 atcgtggatc gagcacggac ccacgacgac gaacagccgt gggtcttcgc ggtcgaggat
     2281 gcggcgcacc gcgtgccggc tgcggtagac gaactcctcc gccgcggcgg gcatggccag
     2341 gtcgcgcttg atcgtctccg gtgtcggcag cagctcgctc gagacgacgt tgatgtcgaa
     2401 gagtctttcg gtcattggct ggtccggacg ggccgggagg cccgcaaccg cttgattcta
     2461 cacaccctca gatcgaggta cgcacgttcc agagctcggg gaagaaacgc aggtcgagcg
     2521 cgcgggcaag gtaggagacc ccgctggagc cgcccgtgcc acgcttgtag ccgatgatcc
     2581 gctccacggt cttcaggtgc gcgaaccgcc acagctggaa gcgctggtcc aggtcgacga
     2641 gcttctcggc gagttcgtac aggtcccagt gctgctcgac gttgcggtag acctcgagcc
     2701 acgcggccgt cacgtcgggg ctctcgacgt agggcacgct ccagtcacgc tcgacgtagt
     2761 gcgcgggaat cgcaaagcca cgacgggcga gcagccgcag gctttcgtcg tagaggctcg
     2821 ggctgtacag cgcctccttc agctgcgcgt agagctcggg gaggtgacgg tgcacctcga
     2881 tcatcgccgg attcttgttg ccgatgatgt actcgagcgt gcggtactgg tagctctgga
     2941 accccgacga cttgccgagc gagtcccgga acgcggaata gtccgccggc gtcatcgtgc
     3001 tcagcacgtc ccacgactgc gccagctgga cctgcacgcg cgagacgcgc gccagcatct
     3061 tgaacgccgg cccgaggtcg tcgtgccgga tctgctcgac cgccgcgcgc agctcgtgga
     3121 tgcagagctt catccagagc tcggtcgcct ggtgcatcac gatgaagagc atctcgtcgt
     3181 gatgggtcga gagcggcgac tgcgcgccga gcaccttgtc gagctgcagg tactggccgt
     3241 agctcagcga ttcgccgagg tcccagtgga tgtcctcacc ctcgaccccg acgtgcgaac
     3301 cgagcgcggg cctgcgtggc cccttcgggt cgtcgggggt gctcatccga acacccatgc
     3361 cggcacgttg cgccagccct gcagcagctc gacgtcgagg ggcttccagt ccggctcgaa
     3421 cgacgcgacc agcgaccaga accgcttcga gtgattcatg tgccggcggt ggcaaagctc
     3481 gtggatcatc aggtagcgca ccacgtccgg tcgctggaac atcaggcaga cgttcaggct
     3541 gatcgtgccc ctggtcgagc agctgcccca gcgcgtgcgc tgccggcgca cgctcagccg
     3601 ggcgaagtcg atgccgatct cgcgtgagag cgcgtccagc cgcaggcgca gctcacgcgt
     3661 ggcgagcctg gtgagccagg gcacgagcgc ctggctcgcg tgccgatcgc tgacgctcgg
     3721 cacgtgcagg cgcaggcggt cgtccccaac ttcttcggcc cggacgcgac gcccgggcag
     3781 gtattcgacc tgccaggcgc gcccgagcag ctccatctcg acctgcgcgg gctggcgctc
     3841 ggcggccttc ggtgcgagcc gcagcaattc gcgcgagcga cgttcggccc agtcgcgatg
     3901 acgcgccacg aagcgctcga tggcgggcac gccggccccg ggcggcacga cgacctcgac
     3961 gcttccgccc gggaacacgc gcatcgacat gcggcgcgcg cgccggctga tgcggacgtt
     4021 ccaggccgtg ccgacctcgc gatcggacca cagcgacaac tgcatcggct ggcccgggcc
     4081 cggcgccgtg ccggccctgc cacgccccag gctactcatc gggtggcgac acctacagtt
     4141 ccctcagtcc cgtcgtgacg ggcatccgtg cggccctgat ccccggcagc aggccgccca
     4201 cgagaccgag tatgagtgaa tagccgaggc cggtcacgag cagggacggc gtcacggtga
     4261 aggcgaacgt gagctggctg aagctgcccc agttgagcgt ggaagcctgg tagccgttca
     4321 gcacgaggta cacgagcagt ccgccgaccg caccgccggc gaggcccagc acggcagact
     4381 cgagcagcac cgacgtgacc accggaccgg caccgaagcc gagcgcgcgc agcgtggcga
     4441 tctcacgcgt ccgcgacgag accgcggaat acatggtgtt gagcgccgcg aacaccgcgc
     4501 cgatgcccat cagcaccgcg ag
//
LOCUS       DASPFR010000226         5514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_673116, whole genome shotgun sequence.
ACCESSION   DASPFR010000226 DASPFR010000000
VERSION     DASPFR010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673116"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VD737_05785"
     CDS             <1..319
                     /locus_tag="VD737_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10102.1"
                     /translation="GDDFKVECPTGSGRYLTIEQVADELSRRLCRIFLKDEQGRRPLY
                     GHHPRLQQDPHFRDHLLFYEYFHGDTGRGVGANHQTGWTGLVAKLLKPRAHDASASSS
                     TDH"
     gene            349..1209
                     /locus_tag="VD737_05790"
     CDS             349..1209
                     /locus_tag="VD737_05790"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXW10103.1"
                     /translation="MDMPVTYAHLPKPVMPNCPVQRTLVGQVAIVTGASSGIGRAIAV
                     TLAQAGADVVVNYTAGEAKALQACETIRAEGGRAIAVRADVSSESDVQAMFRRTIDEF
                     GTVDVLVNNAGLQQDAPFEELTLEQWNRVLGVNLTGQFLCSREAVREFKRRGVRPDVS
                     CSAGKIICVSSVHEVIPWAGHVNYAASKGGVMLMMKSIAQEVAPYRIRVNSICPGAIR
                     TPINMQAWDTAEAYDELLRLIPYRRIGEPEEIGRAAVWLASDFSDYVHGVSLFVDGGM
                     TLYPGFETGG"
     gene            1226..2083
                     /locus_tag="VD737_05795"
     CDS             1226..2083
                     /locus_tag="VD737_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HXW10104.1"
                     /translation="MTDTALSMRTALREHWPEYLIEGWALGTFMVAAALAATLLDSPS
                     SPVHKAIDDPDLRRALAGLAMGLTAMALIYSPWGRRSGAHMNPAVTLTFWRLGRVRHW
                     DAVFYVGAQFVGGTLGVLVAATALGAPFTDPPVNYAATVPGAAGVTIALLAEFAISTV
                     MMFTVLTVSGSAYMKYTGVCAGVLVATWITFEGPFSGMSMNPARTFASALPGGAWGTF
                     WLYVVAPVAGMQLGALLHTFALRERARGCAKLCHGERERCIHCGYPGTTTPPLPRHVA
                     AGEVTGTRA"
     gene            2080..3591
                     /locus_tag="VD737_05800"
     CDS             2080..3591
                     /locus_tag="VD737_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HXW10105.1"
                     /translation="MNTPDPNRLYDAVIVGSGAGGAAAAHTLVRAGLDVLLLEKGREL
                     PTDGSTLDFRAVVHEGRFKSREAWRDGRNRELVPEEYFNVGGKTRWFGAALLRYSESE
                     FDADDSHQCRAWPIQYRDMLPWYQQAEQQLGVRRFACEPALARIVARLSARRSGWQDL
                     PLPLGLDEHIVEHDLEARHFDGFASVAGHKGDANRAFLSPIAGHGNFTLATGEEVTGL
                     MSSPGRPTHVVGVRTASGITFTAREVLLAAGALHTPRLVARHLLEHGLERTLPAARHV
                     GRNLKLHLLTAMLAFGVERPSDVLRKTTYFTHPDLPHSSVQPLGFDGELLGTLIPRIV
                     PRAVARRLGAHAYGFFLQTEDGSHPDNRVLDGGRGANPVIDYDDARVGPARLEHRRLV
                     SRLQRSLAGTGLIAFSQRIGLAGTAHACGTMIAGHDPAESAVDGWGRIHGMRGVRVVD
                     GSVLPRSSRVNPSLSIFGWSLRSATQLAQALREDTSAFDAVTDAVASGTMAHA"
     gene            3588..4313
                     /locus_tag="VD737_05805"
     CDS             3588..4313
                     /locus_tag="VD737_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10106.1"
                     /translation="MSAVPGREVAAPRRRTSRPRASRTNALREAVALVLVALAENRRN
                     RDRALLVAISGVDGSGKTRYAAELAEGLRRAGLCVAVVGVDPWQNPQSERFTSGSEPA
                     DHFYRRAIRFEELFARVVEPLVTQRSLHLETLGIRTDADVWEPLTYEFADVDVVLLEG
                     IFLFRRDLASRYDLRLWVHCPLPTALRRALARNVERRTEPELRRDYARIYHAAQRVHF
                     ELDDPEAGADLVIDNTPWDEELA"
     gene            4313..5350
                     /locus_tag="VD737_05810"
     CDS             4313..5350
                     /locus_tag="VD737_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXW10107.1"
                     /translation="MRSALYRIWLTALLAFAALSTTVGHTASPTEASAAPVRSVDSVG
                     ITVSDMERSLEFYTRVLSFEHVGGREVAGDAYDRLYGVSGLRLRIERLRLGEEHVELM
                     QFLAPRGRPIPADSRSHDRWFQHVAIIVSDMDRAYAVLREHGVEHASIAPQRLPDWNP
                     NAGGIEAFYFRDPDGNHLEILRFPPGKGEARWQQDGRLFLGIDHTAIVVGDTDTSLRF
                     YRDALGLRVAGASENFGIEQERLNNVAGAHLRITALRAASGPGIEFLEYLAPRTGRPM
                     PADTLPNDLWHWQVNTTADVEAARARLAGARPGSDGTQATLPDEHTTAQLVRDPDGHA
                     LLLQQPPSASN"
BASE COUNT          823 a         1980 c         1871 g          840 t
ORIGIN      
        1 cggcgacgac ttcaaggtcg agtgcccgac cggctcgggt cgttacctga cgatagagca
       61 ggtggcggac gagctctcgc ggcgtctctg ccggatcttc ctgaaggacg agcagggacg
      121 gcgcccgctc tacggccatc acccgcggct gcagcaggac ccgcacttcc gcgatcactt
      181 gctgttctac gagtacttcc acggcgacac gggccgcggc gtcggcgcca accaccagac
      241 cggctggacc ggcctcgtgg caaagctgct caagccgcgt gcccacgacg cctccgcctc
      301 ttcttcgact gaccactgac cactaaccac taaccactgg atttccacat ggacatgccc
      361 gtcacctacg cccacctgcc caagcccgtc atgccgaact gtccggtgca gcgcacgctg
      421 gtcggccagg tcgcgatcgt caccggcgcg agttccggca tcggccgcgc catcgccgtg
      481 acgctcgcgc aggccggcgc ggacgtcgtc gtcaactaca ccgcgggcga ggcgaaggcg
      541 ctgcaggcct gcgagacgat ccgcgccgag ggcggccgcg cgatcgccgt ccgtgccgac
      601 gtgtcgagcg agtccgacgt gcaggcgatg ttccggcgca cgatcgacga gttcggcacg
      661 gtcgacgtcc tcgtcaacaa cgccggcctg cagcaggacg cgccgttcga ggagctgacg
      721 ctcgagcagt ggaaccgcgt gctcggcgtc aacctcaccg gccagttcct ctgctcccgt
      781 gaggccgtgc gcgagttcaa gcggcgcggc gtgcgccccg acgtctcgtg ctccgccggc
      841 aagatcatct gcgtgagctc ggtgcacgag gtcatcccct gggcgggaca cgtcaactac
      901 gcggcctcga aaggcggcgt catgttgatg atgaagagca tcgcccagga ggtggcgccg
      961 tatcggatcc gcgtcaacag catctgccct ggcgcgatcc gcacgccgat caacatgcag
     1021 gcgtgggaca cggccgaggc gtacgacgag ctgctgcgcc tgatcccgta ccggcgtatc
     1081 ggcgaaccgg aagagatcgg ccgggcggcc gtctggctcg cctcggactt ctcggactac
     1141 gtgcacggcg tgagcctgtt cgtcgacggc ggcatgacgc tctaccccgg cttcgagacg
     1201 ggcggctgag gccaggagtc gcaccatgac ggacactgca ctatcgatgc gcacggcgct
     1261 gcgcgagcac tggcccgaat acctgatcga gggctgggca ctcggcacgt tcatggtcgc
     1321 ggcggccctg gccgcgacgt tgctcgattc gccgagctcc cccgtgcaca aggcgatcga
     1381 cgacccggac ctgcgccggg cgctcgcggg tctcgcgatg gggctcacgg cgatggccct
     1441 gatctattcg ccctggggtc gccgctccgg cgcgcacatg aacccggcgg tgacgctgac
     1501 gttctggcga ctgggccgcg tgcgtcactg ggacgcggtg ttctacgtcg gcgcgcagtt
     1561 cgtgggtggc acgctcggcg tgctggtcgc cgcgacggcg ctcggggcgc cgttcaccga
     1621 cccgccggtc aactacgccg cgacggtgcc cggcgcggcc ggcgtgacga tcgcgctcct
     1681 ggccgaattc gcgatctcga cggtgatgat gttcaccgtg ctcaccgtct ccgggtccgc
     1741 gtacatgaag tacacgggcg tctgcgccgg ggtgctggtc gcgacgtgga tcacgttcga
     1801 agggccgttc tccggaatga gcatgaaccc tgcgcgcacc ttcgcctcgg cgctgccggg
     1861 cggcgcctgg ggtacgttct ggctctacgt cgtcgcgccc gtcgccggca tgcagctcgg
     1921 tgccttgctg cacacgtttg cactccgcga acgcgcgcgc ggctgcgcca agctctgcca
     1981 cggcgaacgc gagcgctgca tccactgcgg ctaccccggt acgaccaccc cgcccctgcc
     2041 gcgtcacgtg gcggcaggcg aagtcacagg tacacgcgca tgaacactcc cgaccccaac
     2101 cgcctgtacg acgcggtgat cgtgggctcc ggcgccggcg gtgccgccgc cgcgcacacg
     2161 ctcgtgcgcg ccgggctcga cgtgttgctg ctcgagaagg gacgcgagct gcccaccgac
     2221 gggtcgacgc tcgacttccg ggccgtcgtg cacgaggggc ggttcaagag tcgcgaggcg
     2281 tggcgcgacg ggcgtaatcg cgaactggtg cccgaagagt acttcaacgt gggtggcaag
     2341 acgcgctggt tcggcgcggc gctgctgcgc tactccgaga gcgagttcga cgcggacgac
     2401 tcgcaccagt gccgcgcctg gccgatccag tatcgcgaca tgctgccctg gtaccagcag
     2461 gccgaacagc agctcggcgt gcgtcgattc gcctgtgagc cggccctcgc ccgcatcgtc
     2521 gcgcgcctct cggcccgtcg cagcggctgg caggacctgc cgctgccgct cgggctcgac
     2581 gagcacatcg tcgaacacga cctcgaggcc cggcacttcg acggcttcgc ctccgtggcc
     2641 gggcacaagg gcgacgcgaa tcgcgcgttc ctgtcgccaa tcgccggcca cggcaacttc
     2701 acgctcgcga ccggcgagga agtgaccggg ctgatgtcga gccccggtcg ccccacccac
     2761 gtcgtcgggg tgcgcacggc gtccggcatc acgttcacgg ctcgcgaagt gctgctcgcg
     2821 gccggcgcgc tgcacacgcc gcggctcgtc gcgaggcacc tgctcgagca cggccttgaa
     2881 cggaccttgc cggctgcgcg acacgtcgga cgcaacctca agctgcacct gctgaccgca
     2941 atgctcgcgt tcggggtcga acgaccgagc gacgtgttgc gcaagaccac ctacttcacg
     3001 catcccgacc tgccgcattc cagcgtgcag cccctcgggt tcgatggtga gctgctcggc
     3061 acactgatcc cccggatcgt gccgcgcgcc gtcgcgcgac ggctcggcgc gcacgcctac
     3121 ggattcttcc tgcagaccga ggacggttcg cacccggaca accgcgttct cgacggcggc
     3181 cgcggcgcga accccgtcat cgactacgac gacgctcggg tcgggcccgc ccggctcgag
     3241 caccgtcgac tcgtgagccg cctgcagcgc tcgctcgccg ggaccgggct gatcgcgttc
     3301 tcgcagcgga tcgggctggc cggcaccgcc cacgcgtgcg gaacgatgat cgccggtcac
     3361 gatccggccg aatccgcggt cgacggctgg gggcgcatcc acggcatgcg cggggtgcgc
     3421 gtggtcgacg gcagcgtgct gccgcggtcg agccgagtga acccgtcgct gtcgatcttc
     3481 ggctggagcc tgcgatcggc gacgcagctc gcccaggccc tgcgcgagga cacgagtgcg
     3541 ttcgatgcgg tcaccgacgc ggtggcgtcc ggcacgatgg cacacgcatg agcgccgtgc
     3601 caggtcgcga agtggcggcg ccgcgccggc gtacgagccg accccgtgcc agtcgcacca
     3661 acgccttgcg cgaggcggtc gcactcgtgc tcgtcgcact cgcggagaac cggcgcaatc
     3721 gtgaccgcgc gctgctcgtg gcgatcagcg gcgtcgacgg ctcgggcaag acgcgttacg
     3781 cggcggagct cgccgagggg ctgcgcaggg ccggcctgtg cgtggccgtg gtcggcgtcg
     3841 atccgtggca gaacccgcaa tccgagcggt tcacgagcgg ttccgaacct gcggaccact
     3901 tctaccggcg ggcgatccgg ttcgaggagc tcttcgcgcg cgtcgtcgag ccgctggtca
     3961 cgcaacgctc actgcacctg gagacgctcg gcatccgcac cgatgcggac gtctgggagc
     4021 cgctgaccta cgagttcgcc gatgtcgacg tcgtgctgct cgagggcatt ttcctgtttc
     4081 ggcgcgacct cgcctcgcgc tacgacctgc gactctgggt gcactgcccc ctgcccaccg
     4141 cgctgcgccg cgccctggcg cgcaacgtag agcgccgcac cgaacccgag ttgcgtcgcg
     4201 attacgcgcg catttaccac gcggcacagc gcgtgcactt tgaactcgat gatccggagg
     4261 ctggcgcgga cctcgtgatc gacaacaccc cctgggacga ggagctggcc tgatgcgatc
     4321 cgcgctttac agaatctggc tgacggcgct gcttgccttc gcagcgctgt cgacgacggt
     4381 tgggcacacc gcctcgccga ccgaggcgtc cgccgccccg gttcgcagcg tggactcggt
     4441 cggcatcacc gtgagcgaca tggagcgcag cctcgagttc tacacgcgcg tgctgtcgtt
     4501 cgagcacgtg ggcggtcgcg aagtggcggg cgacgcctac gatcgcctgt acggagtgtc
     4561 cgggttgcga ctgcggatcg agcgcctgcg gctcggtgaa gagcacgtcg aactgatgca
     4621 gttcctcgcg cctcgaggcc gccccatccc ggccgactcg cgcagccacg accgctggtt
     4681 ccagcacgtg gcgatcatcg tgtcggacat ggaccgtgcg tacgcggtgc tgcgcgagca
     4741 cggcgtcgag cacgcctcga tcgccccgca gcgcctgccg gactggaatc cgaatgccgg
     4801 cggcatcgag gcgttctatt tccgcgatcc ggacggcaac cacctcgaga tcctgcggtt
     4861 tccgccgggc aagggggagg cccgatggca gcaggacggg cgcctgttcc tgggcatcga
     4921 ccacaccgcg atcgtggtcg gcgacaccga cacgagcctg cgcttttatc gcgacgcgct
     4981 cggcctgcga gtggccggcg ccagcgaaaa ctttggcatc gagcaggagc gactcaacaa
     5041 cgtcgcgggc gcgcacctgc gcatcaccgc gttgcgtgcg gcgagcgggc ccggcatcga
     5101 gttcctcgaa tacctcgcgc cccgcaccgg tcggccgatg ccggcggaca cgctgcccaa
     5161 tgacctgtgg cactggcaag tcaataccac cgccgacgtc gaagcggcgc gtgcccggct
     5221 ggccggtgca cgtcccggca gcgacgggac gcaagccacc cttcccgacg aacacacgac
     5281 cgcgcagctc gtgcgcgatc cggacggcca cgcgctgctg ctgcagcagc ccccgtccgc
     5341 atcgaactga gctgtgcaag gagacttcga tgcaagccca cgcaggtcac gatcaccgag
     5401 ctgaaacacg tcgcgaggac ttgaagcgcg tcgtcatcgt cggcggcggc ttcgccggca
     5461 tcaccctggc cgagcaactc gagcaccgat tgccgcccgg gtgggcgctc acgc
//
LOCUS       DASPFR010000227         9648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_796799, whole genome shotgun sequence.
ACCESSION   DASPFR010000227 DASPFR010000000
VERSION     DASPFR010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9648
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796799"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..432)
                     /locus_tag="VD737_05815"
     CDS             complement(85..432)
                     /locus_tag="VD737_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXW10108.1"
                     /translation="MDSQWNDNQPIYRQLRDKVVAMILEGTLGEGDALPSVRSVAAEH
                     TLNPLTVLKGYQQLVDEGLVEKRRGRGMFVTTGARTELMRDERERFLAQEWPRVRSTI
                     ERLGLDPKELLKK"
     gene            878..1855
                     /locus_tag="VD737_05820"
     CDS             878..1855
                     /locus_tag="VD737_05820"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HXW10109.1"
                     /translation="MKTPVNVAITGAAGQIGYALAFRVASGQMLGPDQPVNLHLLEIT
                     PALGALQGVVMELNDCAFPTLNRIVATDDARVAFKDCHVALLVGARPRGPGMERKDLL
                     LANAQIFSAQGKALDAVADRNVRVLVVGNPANTNALIAMKNAPGLDHKRFTAMVRLDH
                     NRALSQLAEKTGTHVNDLRKVVIWGNHSATQYPDLHHATVGGKPALSLVDQAWYADTF
                     IPVVQQRGAAIIKARGASSAASAGSAAIDHIRDWVLGTPSGDWVSMAVPSDGSYGIPE
                     GVIYGYPVTCKGGKYEIVQGLEIGEFSRARMDATHKELLEERDGVKDLL"
     gene            1973..3988
                     /locus_tag="VD737_05825"
     CDS             1973..3988
                     /locus_tag="VD737_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HXW10110.1"
                     /translation="MNTERKRRLRDVVFGPPRDPLAPETRKHIALIALLAWIGIGADG
                     LSSSAYGPAEAFVALGDHSQLALYLAIATAFTVFVISLAYNQVIELFPNGGGGYKVAT
                     RLVGPHAGLVAGSALIVDYVLTIAISAASGVDAIFSLLPTVHQVVKLEAAIGVVALLI
                     YLNLRGVRESILLLAPIFIGFCVTHFALIAYGIASHGDGLLPEVRATLAETDELAGNV
                     GWFFVLALFLKAYSLGGGTYTGIEAVSNNVNMLREPRVRTGKWTMFLMAASLSLTAAG
                     IILLYLLWDVQPSRTGETLNAVVFGDIIASLGYGEASSHALLVVVLVFEGALLFVAAN
                     TGFLGGPAVLANMAVDRWVPNQFSALSSRLVTRNGVLLMGAAALAVLVWTEGYVSLLV
                     VLYTVNVFITFALSLLGLTIHWWKQRRDVAHAHRRLALAAFALAIVVSILGVIVVERF
                     LQGGSVTLVITASVVGVGLLIRRHYSRIRERTVALERGRRWQMHDLPGPPHPLQPSAP
                     TAVFLVGASRALGLHTVERVEALFPGHFQNFVFVSVGTVDSEAYGSEQSLRTLQYQTR
                     AVLDALVNYAQCHGKGACWFDGYGADRLRELERLALEVRDQFPNSVYFANRLVFEHDT
                     WWTRWLHNQTPLAMQRLLNLHGLELVILPVMLDGRVLPAAPAVAAHV"
     gene            complement(4001..4423)
                     /locus_tag="VD737_05830"
     CDS             complement(4001..4423)
                     /locus_tag="VD737_05830"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXW10111.1"
                     /translation="MFRRKTDQPSIDVSRLSSLIATGVQIAGDVIVTDGVRIDGQVTG
                     NVIAKPETKGLLVLSEQGRIEGNVRVHDAVVNGTICGDIEVENFIELQAGARVTGAIR
                     YRTLQMTCGARVEGTMVCTAESRAAAPDNVVSLKAAEA"
     gene            complement(4470..4838)
                     /locus_tag="VD737_05835"
     CDS             complement(4470..4838)
                     /locus_tag="VD737_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10112.1"
                     /translation="MRSASSTQTLRIATARLEFSSRRRLAQALHVITVTALVCLVVVI
                     ARHLLADAAATQAAIDDLTKQNSTLRAELARARTELELERSTRAALTRQVADMNAETN
                     ELQSRLAFFSSQSGQSRAPR"
     gene            complement(4840..5754)
                     /locus_tag="VD737_05840"
     CDS             complement(4840..5754)
                     /locus_tag="VD737_05840"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXW10113.1"
                     /translation="MRTIAAGRLLACSAVLAALLMGGCVTLGYFLAQPDPSSMRGFRG
                     MADARHSERVVVADRLGELTGRLTRLEIEAAGLARRLGVVPVVEDSSSKPGAAGADER
                     ADGSPRGGPLLPLAGDADAPRVRRPGLGDVGRGLARVEAGIEVLEASLDRIAGVLADR
                     ELTTMAYPNRPPVQGTMPRISSGFGVRHDPFTGRLARHMGLDIPAVHGTAILASGGGR
                     VISAGYRGPYGKAVVIDHGDGLQTLYGHCSKLYVRTGDLVMPQQKIAAVGSTGRSTGP
                     HLHFEVIRDGQRVAPGQVLSSVLARNTP"
     gene            complement(6018..6674)
                     /locus_tag="VD737_05845"
     CDS             complement(6018..6674)
                     /locus_tag="VD737_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HXW10114.1"
                     /translation="MARAKTRSGAKAKSRTGAKTKSRTTAGSRTTSRRAKAPARRRSP
                     ARMDAIALLKADHRQVETWFEQFEKARSDDRKRDLATNICDALRVHSAIEEEIFYPAF
                     YEATREEDLHHEAIIEHDGAKKLIAEIESSGPDEEYYEARVSVLSEMIKHHVKEEEQR
                     DGMFAKARSSGMDLAALGEQLASRKAELESSPEALKALARGKEAGKGLIARLASAVDS
                     "
     gene            complement(6859..7419)
                     /locus_tag="VD737_05850"
     CDS             complement(6859..7419)
                     /locus_tag="VD737_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HXW10115.1"
                     /translation="MPLDVTLTLSDADLDYFRAVMQAASERGNGRDEAETVRAAARVV
                     ERLHGSTRSPYILRRLDRVARLVQMLEDPQWQLPALERRRVLDGLAYVAEARDLVPDD
                     VPALGLLDDAIMLELVLAELQHELEAYEEFDDYRTTETARAAARTVSRDDWLDARRQA
                     LHARMRERRQRDLARLGGDLQPIMRF"
     gene            complement(7433..7876)
                     /locus_tag="VD737_05855"
     CDS             complement(7433..7876)
                     /locus_tag="VD737_05855"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXW10116.1"
                     /translation="MAFSGLGAGGGGAPHAAPMAEINMIPLVDVMLVLLIIFMITAPL
                     IAHRIKIDLPQASTVLLEEKPDTLVIAIMGDGRTFLNDVEVDRPAMLERLAAEAQRKP
                     QPELHLHVDRTLQFQKLATVMADAQNAGMVKVGIATDPIKGDAAH"
     gene            complement(7895..8671)
                     /locus_tag="VD737_05860"
     CDS             complement(7895..8671)
                     /locus_tag="VD737_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXW10117.1"
                     /translation="MRPTEALTSMSFANFLRQTDEVGIFVFALLVLMSIVSWYFIVLK
                     AARVIRIRSRSHGVVATFWRTPADEAVQYLERQPDWEPFSKIALDGEFAMEHHDQLAQ
                     DRVAGTLHRAEFLDRALRTSINREMERLETGLTFLATVGSTAPFVGLFGTVWGIFHAL
                     VRIGASGETSLDQVAGPVGEALIMTALGLAVAVPAVLGYNLLVRNNRVVIAGFHAFAE
                     DLLAYLTTGARVPSSMDHRARKPRRPASPPEPQRSQQATA"
     gene            complement(8690..9400)
                     /locus_tag="VD737_05865"
     CDS             complement(8690..9400)
                     /locus_tag="VD737_05865"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXW10118.1"
                     /translation="MRIDRYVHDERLTWGAAGVLLLVSAIHAAGIGRLLTLQPDVEDE
                     LRPKLFEISLQKLPPPRLPQELERTPPEPNEAPPEKERQKPRLTRPVPILVDTPTTDP
                     VPPDTEVKPYEIGDELLPGVPGPPMPDGALDEPPAGETGPGGTPYAKLKPLDVVPPLY
                     PPRCRRLGIEGTVRVRVLVGERGRPLEVLVGKSSGDASLDEAALDAVRRWRFEPARRD
                     GTPIRAWATVPIEFRLVD"
BASE COUNT         1381 a         3311 c         3356 g         1600 t
ORIGIN      
        1 ggctagtggt cagtggctag tggttagtgc cagagtgggc ggcgcctgcg ttttcttcac
       61 tagccactag ccactagcca ctagtcactt cttgagcagt tccttcggat ccaggccgag
      121 tcgctcgatc gtcgagcgca cccgcggcca ctcctgcgcc aggaaacgct cgcgctcgtc
      181 acgcatgagt tcggtgcggg cgccggtggt cacgaacatg ccgcggccgc ggcgcttctc
      241 gacgagtccc tcgtcgacga gctgctggta gcccttgagc acggtcagcg gattgagcgt
      301 gtgttcggcg gcgacgctgc gcaccgaggg cagcgcgtca ccttcgccga gcgtgccttc
      361 gaggatcatc gccacgacct tgtcgcgcag ctggcgatag atcggctggt tgtcgttcca
      421 ctgggagtcc atgctgggct ggggtcgagg ggcggcctcg gtggtgcgct gctgtactgt
      481 agcagaacac cgtcggccga cccgtcaagg ccggccgatg gccagcccca ccgccgcggt
      541 cgcatcgaaa gccgacgtgg acattgcctt gggggacgcc gacctcgatg cgaccgcata
      601 acccgcgaac agccacacga ttacggcaag ggtgatggcc agtaacctgt ccgacgtgcg
      661 catgttggcg ggtcctctcg ttggtccgtg atgtatctct gtggtgttgt agttaactac
      721 aacactaaac acttggcaag cccttttgtc gaccaacggg acggacgtgg cttgtccatg
      781 ggcgccgtct cttgcagcgc ggtaaaatca gcgatccaac aacgtcgcgg cgggaccctg
      841 ggccccgtac cggccgaaca aacagcgaga aaatcccatg aagacacccg tcaacgtcgc
      901 catcaccggc gccgcgggcc agatcggcta cgccctcgcg ttccgcgtgg cctccggcca
      961 gatgctcggg ccggaccagc cggtgaacct gcacctgctg gaaatcacgc cggcattggg
     1021 cgcgctgcag ggtgtcgtca tggaactgaa cgactgcgcg ttcccgacgc tgaaccgcat
     1081 cgtggcgacg gacgatgcca gggtcgcctt caaggactgc cacgtcgcgc tgctcgtcgg
     1141 tgcgcgcccg cgtggacccg gcatggagcg caaggacctg ctgctggcca acgcgcagat
     1201 cttctctgcg cagggcaagg cgctcgatgc ggtcgcggac cgcaacgttc gcgtgctcgt
     1261 ggtcggcaac ccggccaaca ccaacgcgct gatcgcgatg aagaatgcgc ctggcctcga
     1321 ccacaagcgc ttcacggcga tggtgcgcct cgaccacaac cgcgcgctgt cgcagctggc
     1381 ggaaaagacc ggcacgcacg tgaacgacct gcgcaaggtc gtcatctggg gcaaccattc
     1441 ggccacccag taccccgacc tgcaccacgc cacggtcggc ggcaagccgg cgctgtcgct
     1501 cgtcgaccag gcctggtacg ccgacacgtt catccccgtc gtgcagcagc gtggtgccgc
     1561 gatcatcaag gcgcgcggcg cgtcgtcggc cgcgtcggcg ggttccgcgg cgatcgatca
     1621 catccgcgac tgggtgctcg gcacgccgtc cggcgattgg gtctcgatgg ccgtgccgtc
     1681 cgacggcagc tacggaattc ccgaaggcgt gatctacggg tatcccgtga cctgcaaagg
     1741 cggcaagtac gagatcgtgc aggggctcga gatcggcgag ttcagccgcg cccgcatgga
     1801 cgccacccac aaggagctcc tcgaagagcg ggacggggtg aaggacctgt tgtaggaagc
     1861 ggtcagcggt tagcggttag tcggcgcctg agggcgccgc tgcggaaaga actcaagcac
     1921 ctacggtgac gggcgacgag ccgcgagact cgtcgctcgc tcactcccaa ccatgaacac
     1981 cgaacgcaag cgtcgcctgc gcgacgtggt cttcggtccc ccgcgcgatc cgctcgcgcc
     2041 cgagactcgc aagcacatcg ccctgatcgc gctgctcgcg tggatcggca tcggcgcgga
     2101 cgggctctcg tcgtccgcct acggccccgc cgaggcgttc gtcgcgctgg gcgatcacag
     2161 ccagctcgcg ctctacctgg cgatcgccac ggccttcacg gtcttcgtga tctcgctggc
     2221 gtacaaccag gtgatcgagc tgttcccgaa cggcggcggt ggctacaagg tcgccacgcg
     2281 cctggtcggg ccccacgcgg gcctcgtcgc cggctcggcg ctgatcgtgg actacgtgct
     2341 cacgatcgcg atctcggcgg cgagcggggt cgatgcgatc ttcagcctgc tgcccaccgt
     2401 gcaccaggtg gtcaagctgg aggctgcgat cggcgtcgtc gcgctgctga tctacctgaa
     2461 cctgcgcggc gtgcgcgagt cgatcctgct gctcgcgccg atcttcatcg ggttctgcgt
     2521 cacccacttc gcgctgatcg cctacggcat cgccagccac ggcgacggac tgctgccgga
     2581 ggtgcgcgcg accctggcgg aaaccgacga gctcgccggc aacgtcggct ggttcttcgt
     2641 gctggcgctg ttcctgaagg cgtattcgct cggtggcggc acctacaccg gcatcgaggc
     2701 cgtctcgaac aacgtcaaca tgctgcgcga gccgcgcgtg cgcaccggca agtggacgat
     2761 gttcctgatg gccgcgtcgc tgagcctcac cgccgccggc atcatcctgc tgtacctgct
     2821 gtgggacgtg cagccatccc ggaccggcga gacgctcaac gccgtcgtct tcggcgacat
     2881 catcgcgagc ctgggttacg gcgaggcctc gagtcacgca ctgctcgtgg tcgtgctcgt
     2941 gttcgagggc gcgctgctgt tcgtggccgc caacacgggc tttctcggtg gcccggcggt
     3001 gctcgccaac atggccgtcg accgctgggt accgaaccag ttcagcgcgc tgtcgtcgcg
     3061 gctggtgacg cgcaacggcg tcctgctgat gggcgcggca gcgctcgcgg tgctcgtgtg
     3121 gacggaaggc tacgtgtcgc tgctggtcgt gctctacacc gtgaacgtgt tcatcacgtt
     3181 cgcgctctcg ctgctcggcc tgacgatcca ctggtggaag cagcgacgcg acgtcgcgca
     3241 cgcgcatcgc aggctcgcgc tggcggcctt cgcgctggcg atcgtggtgt cgatcctggg
     3301 cgtcatcgtc gtcgagcggt tcctgcaggg cggcagcgtg acgctcgtga tcacggcgag
     3361 cgtggtcggc gtcggcctgc tgatccgacg ccactactcg cgcattcgcg agcgcaccgt
     3421 ggcgctcgag cgcggccgtc gctggcagat gcacgacctg ccgggaccgc cgcatccact
     3481 gcagccgtcc gcgccgacgg ccgtgttcct ggtgggggcc agccgcgcgc tcggcctcca
     3541 tacggtcgag cgcgtcgaag cgctgttccc cggccacttc cagaacttcg tgttcgtgag
     3601 cgtcggcacc gtcgacagcg aggcctacgg cagcgagcag tcgctgcgga cgctgcagta
     3661 ccagacccgc gcggtgctcg atgcgctcgt caactacgcg cagtgccacg gcaagggcgc
     3721 ctgctggttc gacggctacg gggcggatcg cctgcgcgaa ctcgaacggc tggcgctcga
     3781 ggtgcgcgac cagttcccga acagtgtcta cttcgccaac cgcctggtgt tcgagcacga
     3841 cacgtggtgg acgcgttggc tgcacaacca gacgccgctc gccatgcagc ggctgctcaa
     3901 cctgcacggg ctcgagctgg tgatcctgcc ggtgatgctc gacggtcgcg tgctgccggc
     3961 cgctccggcc gtggcggccc acgtctagcg gcagaccggg ctacgcttcg gcggccttga
     4021 ggctgaccac gttgtcgggc gccgcggcgc gtgactccgc cgtgcacacc atcgtccctt
     4081 cgacgcgcgc cccgcacgtc atctgcagcg tgcggtagcg gatcgcgcca gtgacgcgcg
     4141 cgccggcctg cagttcgatg aagttctcga cttcgatgtc gccgcagatg gtgccgttca
     4201 cgacggcgtc gtgcacgcgg acgttgccct cgatgcggcc ctgttcactg agcacgagca
     4261 ggcccttggt ctccggcttc gcgatcacgt tgccggtcac ctgcccgtcg atgcgcacgc
     4321 cgtccgtcac gatcacgtcg cccgcgatct gcacgccggt ggcgatcagg ctcgacaggc
     4381 gcgagacgtc gatggagggc tggtcggtct ttctgcggaa catcgaagta ctcctcggca
     4441 ggcgattcgt ggcggcgcgg cggcgaagct cagcgcggcg cgcgcgattg gccgctctgc
     4501 gaactgaaga acgcgagccg gctctgcagc tcattggtct cggcgttcat gtcggcaacc
     4561 tggcgcgtca gcgctgcacg cgtggaacgc tcgagctcga gttcggtccg cgcccgtgcg
     4621 agttcggccc gcaacgtcga gttctgtttc gtgaggtcgt cgatcgcggc ctgtgtcgcg
     4681 gcggcgtccg ccagcaggtg ccgggcgatc acgacgacga ggcagaccag cgcggtcacg
     4741 gtgatcacgt gcagggcctg ggccagccga cgccggctgc tgaactcgag gcgcgcggtc
     4801 gcgatccgga gcgtctgcgt gctggaggcc gagcgcattt cagggtgtgt tgcgggcgag
     4861 cacggacgag agcacctggc ccggcgcgac gcgctggccg tcacggatca cttcgaaatg
     4921 caggtgcggc ccggtggagc ggccggtcga gcccaccgcg gcgatcttct gttgcggcat
     4981 gacgaggtcg ccggtgcgca cgtagagctt cgagcagtgg ccgtagagcg tctgcagtcc
     5041 gtcgccgtgg tcgatcacca ctgccttgcc gtacggcccg cggtagcccg cggaaatcac
     5101 gcgcccgccg ccgctcgcga gtatcgccgt gccgtgcacg gcggggatgt cgagccccat
     5161 gtgccgcgcg agtcgcccgg tgaacgggtc gtgccgcacg ccgaagcccg aactgatgcg
     5221 tggcatcgtg ccctgcacgg gcgggcggtt cgggtaagcc atcgtcgtca gttcgcggtc
     5281 cgcgagcacg cccgcgatgc ggtcgagcga agcttccagc acctcgatcc cggcttcgac
     5341 ccgcgcgagg ccgcgaccga cgtcgcccag tccggggcgg cggacgcgcg gcgcatcggc
     5401 gtctcccgcc aggggcagca gcggcccgcc ccgcggcgag ccgtcggcgc gctcgtccgc
     5461 gcctgcggct cctggcttcg aggaggaatc ctccaccaca ggcacgacgc cgagcctgcg
     5521 cgcgaggccc gcggcttcga tctcgagtcg ggtcaggcgg ccggtcagct cgccgagacg
     5581 gtcggctacg acgacgcgct ccgaatgacg ggcatccgcc attccgcgga acccgcgcat
     5641 cgacgacgga tccggttgcg cgaggaaata acccagcgtg acgcagccgc ccatcagcag
     5701 cgccgccagc actgcgctgc aggccagcag ccggcctgcg gcgatggtcc gcatccgaca
     5761 cgcggtcacc ggaccggctg cgtaaatgat gaaagccacg ggctccccac gagtgctcgt
     5821 gcctacttaa cctccgcgga ccggccgaga caacctcctg gccggcggat gcgccaatgt
     5881 gtgatgggct ccccaaatgg cgggcgcgcc tatggcgcac gcgttccacg caatgcccgc
     5941 ttcgtcgtag gccactcgtg ctcgatccgg ccgatccggg cgtgcacggc acgccgccgg
     6001 cgcccgcgcg acgtcggtca gctgtcgacc gcggacgcga gtcgcgcgat cagccccttg
     6061 ccggcttcct tgccgcgcgc gagcgccttg agggcctccg gcgaggactc gagctccgcc
     6121 ttgcgcgagg ccagctgttc gccgagcgcc gccaggtcca tgcccgagct gcgcgccttg
     6181 gcgaacatgc cgtcgcgctg ctcctcttcc ttgacgtggt gcttgatcat ctccgacagc
     6241 acgctcacgc gcgcctcgta gtactcctcg tcgggacccg acgactcgat ttccgcgatc
     6301 agcttcttgg cgccgtcgtg ctcgatgatc gcctcgtggt ggaggtcctc ttcccgggtc
     6361 gcctcgtaga acgccgggta gaagatttct tcctcgatgg cgctgtgcac ccgcagcgcg
     6421 tcgcagatgt tggtggcgag gtcgcgcttg cggtcgtcgc tgcgcgcctt ctcgaactgc
     6481 tcgaaccagg tctcgacctg gcggtgatcg gccttcagga gcgcgatggc gtccatgcgg
     6541 gccgggctgc gccgtcgggc cggtgctttc gcgcgccgcg aggtggtgcg tgatcctgcg
     6601 gtcgtgcggg acttcgtttt cgcgccggtg cgggacttcg ccttcgcgcc gctgcgggtc
     6661 tttgctcgtg ccatgggtgt gctcctcgta aagatccacg cacggtacgg gttcgacgcg
     6721 acgcgacaca gggccgacgc ggccgcctgc gcgtcaggat tggccaggtc gggagcagcg
     6781 aagctgccga cgccgtgggg gacgtcgccg gctccacgtc ggacgtcttc ccctcgccga
     6841 cgccgcgatg cgttctgatc agaaccgcat gatgggctgc aggtcgccgc cgaggcgcgc
     6901 gaggtcgcgc tggcgtcgtt cgcgcatgcg tgcgtgcagc gcctggcgtc gtgcatcgag
     6961 ccagtcgtcc cgcgaaaccg tgcgcgccgc cgcacgggcg gtttcggtcg tgcggtagtc
     7021 gtcgaactcc tcgtaagcct cgagctcgtg ctgcaactcg gcgagcacca gttcgagcat
     7081 gatcgcgtcg tcgagcaggc cgagcgccgg cacgtcgtcg ggcacgaggt cgcgtgcctc
     7141 cgccacgtag gcgaggccgt ccagcacgcg gcggcgctcg agcgcgggca gctgccactg
     7201 cggatcctcg agcatctgga ccaggcgcgc gacgcgatcg aggcggcgca ggatgtacgg
     7261 cgagcgagtc gacccgtgga gcctctcgac cacccgggcc gccgcccgca ccgtctccgc
     7321 ctcgtcgcga ccgttgccgc gctcgctggc ggcctgcatg accgcccgga agtagtcgag
     7381 gtcggcatcc gagagcgtca gggtcacgtc gagcggcatg gtcggatcgc gttcagtgag
     7441 ctgcgtcgcc cttgatcgga tcggtcgcga tgccgacctt gaccatgccg gcgttctgcg
     7501 cgtccgccat caccgtcgcg agcttctgga actgcagcgt cctgtcgacg tgcaggtgga
     7561 gctcgggctg cggcttgcgc tgcgcctcgg cggccagacg ctcgagcatc gccggacggt
     7621 cgacttcgac gtcgttcagg aacgtccggc cgtcgcccat gatcgcgatc acgagcgtgt
     7681 cgggcttttc ctcgagcagc accgtcgacg cctgcggcag gtcgatcttg atccggtgcg
     7741 cgatcagcgg tgccgtgatc atgaagatga tcagcagcac cagcatcacg tcgacgagcg
     7801 ggatcatgtt gatctcggcc atcggcgcag cgtgcggcgc acctccgccg ccggccccga
     7861 gcccactgaa cgccatgtct gcctcccgga cggctcaggc cgtcgcctgc tgcgatcgtt
     7921 gcggctcggg cggcgatgcc ggtcgccgcg gcttgcgggc ccgatggtcc atcgacgagg
     7981 gcacgcgcgc gccggtcgtc aggtacgcga gcaggtcctc ggcgaatgcg tggaagcccg
     8041 cgatgacgac ccggttgttg cgcaccagca ggttgtagcc gagcacggcg ggaacggcca
     8101 cggcgaggcc gagcgcggtc atgatcagcg cctcgccgac gggcccggcc acctggtcga
     8161 gcgaggtctc cccggacgcg ccgatgcgga ccagcgcgtg gaagatgccc cagaccgtcc
     8221 cgaacaaccc tacgaacggc gcggtgctgc ccaccgtcgc gaggaacgtg agccccgtct
     8281 cgagtcgttc catctcgcgg ttgatcgagg tccgcagcgc ccggtcgagg aactcggcac
     8341 ggtgcagcgt gcccgccacc cggtcctggg cgagctggtc gtgatgttcc atcgcgaact
     8401 cgccgtcgag cgcgatcttc gagaacggtt cccagtccgg ttgtcgctcg aggtactgga
     8461 cggcttcgtc ggcaggcgtg cgccagaacg tcgcgacgac gccgtggctg cgcgagcgta
     8521 tgcgtatcac gcgcgcggcc ttgagcacga tgaagtacca ggacacgatc gacatcagca
     8581 cgagcagcgc gaacacgaag atgcccacct cgtcggtctg gcgcaggaag ttcgcgaaac
     8641 tcatcgatgt cagggcttcg gtgggacgca tgtggggatc cccgggtcgt caatcgacca
     8701 gtctgaattc gatgggcacg gtggcccagg cgcggatggg cgtgccgtcg cgccgcgcgg
     8761 gctcgaagcg ccacctgcgc acggcgtcca gcgcggcttc gtcgagcgag gcgtcgccgc
     8821 tgctcttgcc gacgagcact tcgagcgggc gcccccgctc gccgacgagc acgcgcacgc
     8881 gcaccgtgcc ctcgatgccg aggcgtcggc agcgcggtgg gtagagcgga gggacgacgt
     8941 cgagcggctt cagcttcgcg tacggcgtgc cgccggggcc ggtctcgccc gcgggcggtt
     9001 cgtcgagcgc gccatccggc atcggcgggc cgggcacgcc cggcagcaac tcatcgccga
     9061 tctcgtaagg cttgacctcg gtatcgggcg gtaccgggtc cgtcgtcggc gtgtcgacga
     9121 ggatcggcac ggggcgcgtc aggcgcggct tctggcgttc cttctccggc ggtgcctcgt
     9181 tgggttcggg cggcgtccgc tcgagttcct gcggcaggcg gggcggcggc agtttctgca
     9241 ggctgatctc gaacagcttc gggcgcaact cgtcttcgac gtccggctgc agcgtgagca
     9301 ggcgaccgat gcccgcggcg tgaatggcac tcaccagcag caagacgccc gcggcacccc
     9361 aggtgaggcg ttcgtcgtgg acgtagcggt cgatgcgcat cggtgaccgg ccaggccggc
     9421 aaatgcttgc ttggtatcga gtgcgactct agcccaagcg ccgcgcgacg acgccgagcg
     9481 gaagtaccta gcctgggtgc gcctcgcgca ggggcgtgga ccgtgtctcg tcgagcggtg
     9541 gcggcagggt cttgtcagcg gtggcgcggc cgggtgtcgg tcgccgggcc gactcgaagc
     9601 cgcgtccttg cggcagcgca gcatgcgagc gcatagtcac cgcggacg
//
LOCUS       DASPFR010000228         3052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_308675, whole genome shotgun sequence.
ACCESSION   DASPFR010000228 DASPFR010000000
VERSION     DASPFR010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308675"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..497)
                     /gene="crcB"
                     /locus_tag="VD737_05870"
     CDS             complement(93..497)
                     /gene="crcB"
                     /locus_tag="VD737_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HXW10119.1"
                     /translation="MNLSLPLHAYLLVAFGSGLGGAARLWVSTLVSRGTGTAFPWGTL
                     AVNVAGCLIVGIMAALFEPASPLHVRQDLRVLLVVGLLGGFTTFSAFSLETLLLIQRG
                     AVAAATAYVVASVALGLLAASAAYMSMAAALR"
     gene            627..1895
                     /gene="hemL"
                     /locus_tag="VD737_05875"
     CDS             627..1895
                     /gene="hemL"
                     /locus_tag="VD737_05875"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HXW10120.1"
                     /translation="MSLFQEACKYIPGGVNSPVRAFRGVGGEPVFFERAKGSRAWSAD
                     GREFIDYVGSWGPMILGHAHPDVIRAVQQAAEHGLSFGAPTAIETTVARKIIELVPSI
                     ELVRMCSSGTEATMSAIRLARGYTGRHKIVKFEGCYHGHSDSLLVKAGSGMLTLGVPT
                     SPGVPPELAAHTLTLSYNDVAQVRQVFAELGGEIACVIVEPVAGNMNCIPPVPGFLEA
                     LREECTKSGAVLILDEVMTGFRVARGGAQELYGIRPDLTTLGKIVGGGMPVGVFGGRR
                     DIMSHIAPLGPVYQAGTLSGNPVAMAAGLATLNGIDAPGFHARLGALTDRLVEGFARA
                     AKQAGVPLATNHVCGMFGLFFTSAPTVTSYADATQCDVERFKKFFHGMLDEGVYLAPS
                     AFEAGFVSIAHEEADIDATVAAAERVFARL"
     gene            complement(1920..>3052)
                     /locus_tag="VD737_05880"
     CDS             complement(1920..>3052)
                     /locus_tag="VD737_05880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXW10121.1"
                     /translation="GGPTAGPDAAPRRIGRGMSPSPSQRPRLRLLHVILSRGFAGSER
                     AAAEACNALARTNDVALVLRRDHRNASGASIRDYVGPDVAVYELPPLWRTRAALADVI
                     DQWRPSLIHTHLRRGTRYVAQLRRGMPHVATLHIDLDGRHYLRTDGLCCISPWQVEKV
                     VGEGYAGAVFQIPNSLVPHPRISPQRRAELRGLAGASPDDFLVGGVGRLAHGKGFDVL
                     IDAIRAARLPRTRLVIVGEGSERSRLERLAADLPVTFTGFRHDAKDWFQALDLFVSAS
                     RSEPFGRVIIEALDAGTPVIASDAVGPRDIAANYPIDVTPAGDVAALAAAIVRAHARP
                     RSRLEVDLGEFHVDRVTESLLEAYHALIGTAQRRPVAPRVAA"
BASE COUNT          460 a         1058 c         1110 g          424 t
ORIGIN      
        1 agcggttagc ggttagcggt tagtccgacc gagcacggcc ggcggctaca tcgttccgac
       61 taaccgctgt tcactaaccg ctaaccgctt cctcagcgca gtgccgcggc catgctcatg
      121 tacgccgcgg atgcggcgag caggcccagc gcgaccgagg cgacgacgta cgcggtcgcg
      181 gcggcgacgg cgccgcgctg gatcagcagc agcgtctcga gcgagaacgc ggagaacgtc
      241 gtgaagccgc cgagcaggcc gaccacgagc agcacgcgca ggtcctgccg cacgtgcagc
      301 ggactcgcgg gctcgaacag cgcggccatg atgccgacga tgaggcagcc cgcgacgttg
      361 accgcgagcg tcccccaggg aaacgcggtg cccgtgccgc ggctcacgag cgtcgagacc
      421 cagaggcggg ccgcgccgcc gagaccgctg ccgaaggcga cgagcagata ggcgtgcagg
      481 ggcagggaga ggttcaacga ggacaccgcc tccgtcgggt gccgaaagga ccgtcggtgt
      541 tttccgcagc atcttagtgc gcggaccggg ccggcgcgag cacgctgcta agattgcgcc
      601 tcgcccgaag ctggagttcc gagcccgtga gcctgttcca agaagcctgc aagtacattc
      661 ccggcggcgt caactcgccc gtgcgcgcgt tccgcggcgt cggtggcgaa cccgtgttct
      721 tcgagcgcgc caagggctcg cgcgcgtggt cggccgacgg ccgcgagttc atcgactacg
      781 tcggctcctg ggggccgatg atcctcggtc acgctcaccc cgacgtgatc cgtgcggtgc
      841 agcaggcggc ggagcacggg ctctcgttcg gcgcgccgac ggccatcgag acgacggtcg
      901 cacgcaagat catcgagctg gtgccgtcga tcgagctggt gcggatgtgc agttcgggca
      961 ccgaggcgac catgagcgcg atccgcctcg cgcgtggcta caccggccgt cacaagatcg
     1021 tcaagttcga gggctgctat cacggccact cggactcgct gctggtcaag gccggctccg
     1081 gaatgctgac gctcggcgtg ccgacgtcgc ccggcgtgcc ccccgaactg gcggcgcaca
     1141 cgctcacgct gtcgtacaac gacgtggcgc aggtgcggca ggtgttcgcg gagctcggcg
     1201 gcgagatcgc ctgcgtgatc gtcgagccgg tggccggcaa catgaactgc atcccgccgg
     1261 tgccgggatt cctcgaggca ctgcgcgagg agtgcacgaa gtccggtgcg gtgctgatcc
     1321 tcgacgaggt catgaccggc ttccgcgtgg ccaggggcgg cgcgcaagag ctctacggca
     1381 tccggcccga cctcaccacg ctcggcaaga tcgtgggcgg cggcatgccg gtcggcgtgt
     1441 tcggcgggcg tcgcgacatc atgagtcaca tcgcgccgct cggccccgtc taccaggcgg
     1501 gcacgctgtc gggcaatccc gtcgcgatgg cggccgggtt ggcgacgttg aacggcatcg
     1561 acgcgcccgg cttccacgcg aggctcggcg cgctgacgga ccgtctcgtc gaagggttcg
     1621 cccgcgccgc aaagcaggcc ggcgtgccac tcgcgacgaa ccacgtctgc ggcatgttcg
     1681 ggctgttctt cacgtcggcg ccgaccgtca cctcgtatgc cgacgcgacg cagtgcgacg
     1741 tggaacgctt caagaagttc ttccacggca tgctcgacga gggcgtctac ctcgcgcctt
     1801 ccgcgttcga ggcgggcttc gtctcgatcg cgcacgagga ggccgacatc gacgcgacgg
     1861 tcgccgcggc cgaacgcgtg ttcgcccggc tctgaccgcg cgagcgggag gtcgatgcgc
     1921 tacgcggcga cgcgcggcgc gaccggccga cgctgcgccg tgccgatcag cgcgtgatag
     1981 gcctcgagca gcgactcggt cacccggtcg acgtggaatt cgcccaggtc gacttcgagc
     2041 cgtgacctcg gccgcgcgtg ggcgcgcacg atcgccgccg ccagtgcggc gacgtcgccc
     2101 gccggcgtga cgtcgatcgg ataattcgcg gcgatgtcgc gcggaccgac ggcgtcggag
     2161 gcgatcaccg gcgtgcccgc atccagcgcc tcgatgatca cgcgcccgaa cggttcggaa
     2221 cgcgatgcgc tcacgaacag gtcgagcgcc tggaaccagt ccttggcatc gtggcggaag
     2281 ccggtgaacg tgacgggcag gtccgcggcg agtcgttcga gccgcgagcg ttcgctgcct
     2341 tcgccgacga tcacgaggcg cgtgcgcggc agccgcgcag cgcggatcgc gtcgatcagc
     2401 acgtcgaagc ccttgccgtg cgcgagacgg ccgacgccgc cgaccaggaa atcgtcgggc
     2461 gacgcgccgg cgaggccgcg cagctccgcg cgcctctgcg gcgaaatacg cgggtgcggc
     2521 acgagcgaat tcgggatctg gaacaccgcg cccgcgtagc cctcgccgac caccttctcg
     2581 acctgccacg gcgagatgca gcacaggccg tcggtgcgca ggtagtgccg cccgtcgagg
     2641 tcgatgtgca gcgtcgccac gtgcggcatg ccgcgccgca actgcgcgac gtaacgcgtg
     2701 ccgcggcgca ggtgcgtgtg gatcagcgac ggtcgccact ggtcgatcac gtccgcgagc
     2761 gcggcgcgcg tgcgccagag cggcggcagc tcgtagaccg cgacgtccgg gcccacgtag
     2821 tcgcggatgc tggcgccgga cgcgttgcgg tggtcgcggc gcaggacgag cgcgacgtcg
     2881 ttggtgcgtg ccagtgcgtt gcaggcttcg gccgccgcgc gctccgagcc cgcgaacccg
     2941 cgcgacagga tgacgtgcag caggcgcagc cgcggtcgtt gcgatggcga ggggctcatg
     3001 ccgcgcccga tccgacgcgg cgccgcgtcc gggccagcag tcgggccacc cc
//
LOCUS       DASPFR010000229         5204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_308760, whole genome shotgun sequence.
ACCESSION   DASPFR010000229 DASPFR010000000
VERSION     DASPFR010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308760"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1199)
                     /locus_tag="VD737_05885"
     CDS             complement(156..1199)
                     /locus_tag="VD737_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXW10122.1"
                     /translation="MPRITLVPTGQSFSSVDDEPVLTAALRAGLNLPHSCKGGHCASC
                     RARILVGDVEYPEGRLPAGITTGEVQERYALLCQAYALVDLTVETREVRPAPEVEVRN
                     LPCRIDRLERLADDVMAVFLKLPAVEELRFRAGQYLDFILPGGRRRSFSIASAPADGR
                     LLAVHVRRASEHGFTGQLFDTMRAGALLRIEGPLGQFWFRTESPRPALMIGGGAGYAP
                     LRAMLRQLLTVGDRRPLEVYWGARTSYDLYEHTWLSNVAVVRPAFDYRPVLSSPEREE
                     RPWCGRTGLVHEAVLSDHAEDLARYDVYASGPPAMIEAIRHQYLARGLPREQLYFDSF
                     DYAPDTLAAMRGK"
     gene            1250..2176
                     /locus_tag="VD737_05890"
     CDS             1250..2176
                     /locus_tag="VD737_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXW10123.1"
                     /translation="MLPGMSNGLLSCLVVGCGYVGARFARQAAGRRPVLGIVRTGPSS
                     SELAAAGINTMRLDLDDTHPVLQPSLATAAAGAAVVYLVPPPDHGTSDPRLERFLLQL
                     GDAVPAVLAYVSTTGVYGDTGGALVDEHAPLAPGNDRARRRVAAESVAQAWCAARGVR
                     CVILRVPGIYGPHRLPLERLERGEPALRPEDAGPGNRIHVDDLVASIAAAIDAPTAQG
                     AYNVTDGDPASTTDFLQQAAAAAGLPPPPLVPRSEAARHIPPGMLSFLLESRRVDNRR
                     LVNELGVRLRYPRAQAGILASLAEMRMEETRP"
     gene            2173..2604
                     /locus_tag="VD737_05895"
     CDS             2173..2604
                     /locus_tag="VD737_05895"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXW10124.1"
                     /translation="MKFGNPSREEIRALLERSRTIAVVGLSDDPARPSHGVTRVMQRF
                     GYRIVPVNPELEQWEGLPAYPDLASAVSGSAEPIDIVNVFRRPARVGTVVDECLRLSL
                     PALWLQVGVVNEEAAARAVAAGMTVVMDRCIKVDRMAMSDR"
     gene            2633..3475
                     /gene="dapF"
                     /locus_tag="VD737_05900"
     CDS             2633..3475
                     /gene="dapF"
                     /locus_tag="VD737_05900"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HXW10125.1"
                     /translation="MRLAFTKMHGLGNDFIVFDAPDAASVPTPGQLRRLADRRTGIGF
                     DQALVLERPRRAGTDVYYRVFNADGSEVEQCGNGARCIARLVASRRHAAREQPLAMDS
                     PGGVVHARLRPDGLVSVAMGVPDFAPASLPFDAPAEAPTYHLDTRAGPVEIAAVSIGN
                     PHAVIRVRDVRLAPVDTVGPAVENHASFPRRVNVGFLEIVAPDRVRLRVHERGVGETQ
                     ACGTGACAAVAVGRQHGPLAEEVTVDVPGGRLIVRWPGPGEQVWLTGPAETAFEGHVD
                     VAGA"
     gene            3492..4196
                     /locus_tag="VD737_05905"
     CDS             3492..4196
                     /locus_tag="VD737_05905"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HXW10126.1"
                     /translation="MTKPIRESSLLAEDQVADYLLAHPEFFERHGTVLARLKLPHQRG
                     SAAVSLVERQVLVLRDKHAVLEKKLHELIENGRTNDSISDRVHRLTRRLLRARDATGV
                     IMGLETSLREDFGASRWVLLATDPALAPLAHLQNPHVRVVPRGSAELRIFEAFFESAR
                     PRSGQIRDTQREYLFGGDGSQVGSTVLIPLGERASLGLLAIASHDTERYLPTMSTDFL
                     VRISEIVTEAVSSRLA"
     gene            4193..5098
                     /gene="xerC"
                     /locus_tag="VD737_05910"
     CDS             4193..5098
                     /gene="xerC"
                     /locus_tag="VD737_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008040629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HXW10127.1"
                     /translation="MTAPAGLAGSVERYLGSLRHERQLSPHTASNYGRDLAALVDYCA
                     TQGLTAWSQLDTQHLRAFAAREHRRGLAPRSIQRRLSACRGLFRYLIREGELRADPAG
                     DVQAPKARKRLPTPLDADSMARLLEFRSDDTLGVRDKAIMELFYSSGLRLAELLGLDL
                     LDLDLRDRTVRVVGKGRKTRIVPVGRHAVEAIRRWLGERATVAGVGEQALFVGQNGGR
                     LGPRIVQRRIARWARLQGLPEHVHPHMFRHSFASHLLESSQDLRAVQELLGHANISTT
                     QVYTHLDFQHLARIYDAAHPRARRK"
BASE COUNT          770 a         1842 c         1859 g          733 t
ORIGIN      
        1 atgcagaact gctcgagcag ctcggcgaag gcgatcgcgc gcgtagcgtg ctgcgcgaca
       61 gcgtcgcgcg ctcgctcggc cgccgtccga cgacgctgcc gagggtcagg ctgaggagaa
      121 gtggctagtg gtcagtggcc actagccacc ggccactact tcccgcgcat ggccgcgagc
      181 gtgtccgggg cgtagtcgaa cgaatcgaaa tagagctgct cgcgcggcag gccgcgggca
      241 aggtactggt gccggatcgc ctcgatcatt gccggcggcc cgctcgcata cacgtcgtac
      301 cgggccaggt cctccgcgtg atcgctgagc acggcctcgt ggaccagacc ggtgcgaccg
      361 caccaggggc gctcctcgcg ctccggtgag gacagcacgg gccggtagtc gaacgcgggc
      421 ctcacgactg ccacgttcga gagccaagtg tgctcgtaca ggtcgtagct ggtgcgggcg
      481 ccccagtaca cctcgagcgg gcgtcggtcg ccgacggtga gcagctgccg cagcatggcg
      541 cgcagcggcg cgtagcccgc gccaccgccg atcatcaggg ccgggcgcgg cgattcggtg
      601 cgaaaccaga actggccgag cggaccttcg atgcgcaaca gcgccccggc gcgcatcgtg
      661 tcgaacagct gccccgtgaa gccgtgctcg gacgcgcgtc gcacgtgcac cgcgagcagc
      721 cgaccgtcgg ccggcgcgct cgcgatcgag aagctgcgcc gtcgcccgcc gggcaggatg
      781 aagtcgaggt actggcccgc gcggaagcgc agttcctcga cggccggcag cttgaggaac
      841 acggccatca cgtcgtcggc aagccgctcg aggcgatcga tccggcatgg caggttgcgg
      901 acctcgacct cgggcgccgg ccgcacctcg cgcgtctcga cggtcaggtc gacgagtgca
      961 taggcctggc acagcagcgc gtagcgctcc tgcacctcgc cggtcgtgat gccggccggc
     1021 aggcgtccct cggggtattc gacgtcaccg acgagaatcc gcgcgcggca ggacgcgcaa
     1081 tgaccgccct tgcaactgtg cggcaggttg agtcccgcgc gcagcgcggc ggtgagtacc
     1141 ggctcgtcgt cgacggacga aaagctctgg ccggtgggaa cgagcgtgat ccgtggcatg
     1201 agtcgtagga attcggcgcg gcggacggcc ccgcaggagg ccgtcgcgta tgctgcccgg
     1261 gatgagcaat ggccttttat catgtctcgt ggtcggctgc ggctacgtcg gcgcgcgctt
     1321 cgcacgtcaa gcggcgggac ggcgtccggt gctgggaatc gtgcgtaccg gaccgagctc
     1381 gtcggaactc gcggcggccg gcatcaacac catgcgactc gacctcgacg acacgcaccc
     1441 cgtgctgcag ccgtcgctcg cgaccgccgc ggcaggggcg gccgtggtgt acctcgtgcc
     1501 gccgcccgac cacgggacga gtgacccgcg actcgagcgc ttcctgctgc agctcggcga
     1561 cgcggtgccg gcggtgctgg cgtacgtgag cacgaccggc gtctacggcg acaccggggg
     1621 cgcgctcgtc gacgagcacg ccccgctcgc gccgggcaac gatcgcgccc gacgccgcgt
     1681 cgcagccgaa agtgtcgcgc aggcctggtg cgcggcccgc ggcgtgcgtt gcgtgatcct
     1741 gcgcgtgccc ggcatctacg ggccgcaccg gctgccgctc gagcggctcg agcgtggtga
     1801 accggcgctg cgcccggagg acgccgggcc gggcaatcgc atccacgtcg acgacctcgt
     1861 ggcgagcatc gcggcagcga tcgacgcgcc caccgcccag ggtgcgtaca acgtgaccga
     1921 cggcgacccg gcgagcacga cggatttcct gcagcaggcc gcggccgcgg cgggcctgcc
     1981 cccgccgccg ctggtgccta gatcggaggc tgcacgccac atcccgcccg gcatgctctc
     2041 gttcctgctc gagtcgcgcc gcgtcgacaa ccggcgcctg gtcaacgaac tcggcgtgcg
     2101 gctgcgctac ccgcgcgcgc aggcgggcat cctcgcgagt ctcgcggaga tgcgcatgga
     2161 ggaaacccgg ccatgaagtt cggaaatccg tcccgcgagg agattcgcgc actgctcgaa
     2221 cgcagccgca ccattgccgt ggtcggcctg tccgatgacc cggcgcgccc cagccacggc
     2281 gtcactcgcg tgatgcagcg tttcggctac cggatcgtgc ccgtcaaccc ggagctcgag
     2341 cagtgggagg gcctgcctgc gtatccggac ctcgcgagcg cggtgagcgg ctcagcggag
     2401 cccatcgaca tcgtcaacgt gttcaggcgg ccggcgcggg tcggcactgt cgtggacgag
     2461 tgcctgaggc tcagcctgcc ggcgctgtgg ctgcaggtcg gcgtcgtcaa cgaggaagcg
     2521 gccgcgcgcg ccgtcgcggc cggcatgacc gtcgtgatgg accgctgcat caaggtcgac
     2581 cggatggcga tgtcggatcg ttgacggccg ggctcgggta tccttggtgg cgatgcgact
     2641 cgcattcacc aagatgcacg gcctcggcaa cgatttcatc gtcttcgacg cgcccgacgc
     2701 cgcatccgtc ccgacccccg gccagctgcg ccgacttgcc gatcgccgta ccggcatcgg
     2761 tttcgaccag gcgctcgtgc tcgaacggcc gcgtcgcgcg ggcaccgacg tctactaccg
     2821 ggtcttcaac gccgacggct ccgaggtcga gcagtgcggc aacggcgcgc ggtgcatcgc
     2881 acggctggtc gcgagccgcc gccacgccgc acgcgagcag ccgctggcga tggacagccc
     2941 tggcggcgtc gtgcacgcac gcctgcggcc ggacggactc gtttcggtag cgatgggcgt
     3001 gcccgacttc gcgccggctt cgctgccgtt cgacgcgccg gccgaggcgc cgacgtatca
     3061 cctcgacacg cgcgcgggcc ccgtcgagat cgccgcggtg tcgatcggca atccgcacgc
     3121 cgtgatccgc gttcgcgacg tcaggctcgc gcccgtggac accgtcggac ccgcagtgga
     3181 aaatcacgcg agctttccgc gccgcgtcaa cgtgggcttc ctggagatcg tcgcgccgga
     3241 tcgcgtgcgg ttgcgcgtgc acgagcgcgg ggtcggcgag acgcaggctt gcggcaccgg
     3301 ggcctgcgcg gccgtggcgg tcgggcggca gcacgggcca ctggccgagg aagtgacggt
     3361 ggacgtgccg ggcggacgcc tgatcgtgcg ctggcccggg cccggcgagc aggtctggct
     3421 cacgggaccg gccgagacgg cttttgaagg ccacgtggac gtggcgggcg cctgaagcgc
     3481 cggagagaag aatgaccaaa ccgatccgcg agtcgagcct gcttgccgaa gaccaggtgg
     3541 ccgattacct gctcgcgcac ccggaattct tcgaacgcca cggcaccgtg ctcgcgcggc
     3601 tcaagctgcc gcaccagcgc ggcagcgccg cggtctcgct ggtcgagcgc caggtgctgg
     3661 tgctgcgcga caagcacgcg gtgctcgaga agaaactgca cgaactgatc gagaacgggc
     3721 gcaccaacga ctcgatctcc gatcgcgtgc accgcctcac gcggcgcctg ctccgggcgc
     3781 gcgacgccac gggcgtgatc atggggctgg agacgtcgtt gcgggaggac ttcggcgcgt
     3841 cgcgctgggt gctgctcgcg accgacccgg cgctcgcgcc gctcgcgcac ctgcagaacc
     3901 cgcacgtgcg cgtcgtgccg cgcggcagcg ccgaactccg gatcttcgag gccttcttcg
     3961 agtccgcgcg accgcgcagc gggcagatcc gcgacacgca gcgcgaatac ctgttcggcg
     4021 gtgacggttc gcaggtcggc tcgaccgtgc tgatcccact gggcgagcgc gcgagcctcg
     4081 ggctgctggc gatcgcgagc cacgacaccg agcgctacct gccgaccatg agcacggatt
     4141 tcctggtccg catcagcgag atcgtcacgg aggccgtgtc gtcgcggctg gcatgacggc
     4201 cccggccggg ctggccggaa gcgtcgagcg ctacctcggc agcctgcgcc acgagcgaca
     4261 gctgtcgccg cacacggcga gcaactacgg gcgcgacctc gccgcgctgg tcgactactg
     4321 cgcgacgcag gggctcacgg cgtggtcgca gctcgacacc cagcacctgc gcgcgttcgc
     4381 cgcgcgcgag caccggcggg gtctcgcgcc gcgcagcatc cagcgccggc tgtcggcctg
     4441 ccgcggcctg ttccgctacc tgatccgcga aggagaactg cgcgccgacc cagccggcga
     4501 cgtgcaggcg ccgaaggccc gcaagcggct gccgacgccg ctggacgcgg attcgatggc
     4561 acggctgctc gagttccgca gcgacgacac gctcggcgtg cgcgacaagg cgatcatgga
     4621 actgttctac tcgtccggcc tgcggctcgc cgagctgctc ggcctggacc tgctcgacct
     4681 cgacctgcgc gatcgcaccg tgcgcgtggt gggcaagggc cgcaagacgc ggatcgttcc
     4741 ggtcggccgt cacgcggtcg aggcgatccg gcgctggctc ggggagcgcg cgaccgtcgc
     4801 cggcgtgggc gagcaggcgc tgttcgtcgg gcagaacggc ggccggctcg ggccgcggat
     4861 cgtgcaacgc cgcatcgcgc gctgggcccg cctgcagggg ttgcccgagc acgtacatcc
     4921 gcacatgttc cggcactcgt tcgcgtcgca cctgctcgag tcgtcgcagg acctgcgtgc
     4981 cgtccaggaa ctgctcggtc acgccaacat cagcacgacc caggtgtaca cgcaccttga
     5041 tttccagcac ctggcccgga tctatgatgc ggcgcaccca cgggcgaggc ggaaatagcg
     5101 gttagcggtt agcggttagc cagaaggcct ccgtgacatc ccagccacaa tcgctcagtc
     5161 tgtaacgatc cgcactgcaa cttaaccgct aaccgctaac cgct
//
LOCUS       DASPFR010000230        11923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1651939, whole genome shotgun sequence.
ACCESSION   DASPFR010000230 DASPFR010000000
VERSION     DASPFR010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11923
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651939"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /gene="pyk"
                     /locus_tag="VD737_05915"
     CDS             complement(<1..872)
                     /gene="pyk"
                     /locus_tag="VD737_05915"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HXW10128.1"
                     /translation="MLSRRTKIVATLGPATDGPGILEDVIRQGADVLRVNFSHGEPGD
                     HRRRVQRAREAAASVGKHVAMLGDLQGPKIRIERFAGGRTVLSEGATFVLDPDLDAHD
                     GTDRGVGITYAELAQDVRPGDELILGDGQIELTVTAVEGRRVVCRVLTGGEVGDHKGI
                     NRRGGGLSAKALTGKDREDVVLGAELDIDYLAVSFVRQAADVEEARELLRQAGSDARI
                     VAKIERSEAIPRLDEIVQASDAVMVARGDLGIEVGYAELTGLQKHILQVARKYDRVTI
                     TATQMMESMIHSPVP"
     gene            complement(947..2128)
                     /locus_tag="VD737_05920"
     CDS             complement(947..2128)
                     /locus_tag="VD737_05920"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004973295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HXW10129.1"
                     /translation="MRVLRMNELDLGGKRVLIREDLNVPVQNGVVTSDARIRASLPTI
                     EAAMKAKARVMLMSHLGRPEEGVPNEEFSLAPVAARLSELLGRKVRFERDWLQNGVTS
                     EPGEVVLLENVRFNKGEKKNKDELAQKMAALCDIYVMDAFATAHRAEASTHGVGKFAP
                     VACAGPLLTNELDALEQALSSPRRPLVAIVAGSKVSTKLTVLENLLGKVDKLIVGGGI
                     ANTFLAAQGHPIGKSLHEAEMIDTARTLLERSKGGGVEIPLPTDVVVAREFAASAEAD
                     VKPVAQVAPNEMILDIGPDTAERFAAALQDAGTIVWNGPVGVFEFDQFGEGTRTIAQA
                     VARSPAFSIAGGGDTIAAIEKYGVAEDISYISTAGGAFLEFLEGKKLPAVEMLEQRAA
                     G"
     gene            complement(2176..3174)
                     /gene="gap"
                     /locus_tag="VD737_05925"
     CDS             complement(2176..3174)
                     /gene="gap"
                     /locus_tag="VD737_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HXW10130.1"
                     /translation="MAIKVGINGFGRIGRMVFRAAVKDFADVEVVGINDLLEPDYLAY
                     MLRYDSVHGRFKGDVSVDGNTLVVNGRRIRLTAVKDPAELKWGDLGADVVVESTGLFL
                     TKETAEKHLAAGAKKVILSAPSKDDTPMFVYGVNHSKYAGEAIISNASCTTNCLAPVA
                     KVLHDTWGIKRGLMTTVHAATASQKVVDGPSNKDWRGGRGILENIIPSSTGAAKAVGK
                     VIPELNKKLTGMSFRVPTSDVSVVDLTVELSKPAKYDEICATMKAASEGAMKGVLGYT
                     EEKVVATDFRGESCTSVFDAEAGIALDDTFVKVVAWYDNEWGYSSKVLEMARVIAK"
     gene            complement(3224..5215)
                     /gene="tkt"
                     /locus_tag="VD737_05930"
     CDS             complement(3224..5215)
                     /gene="tkt"
                     /locus_tag="VD737_05930"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXW10131.1"
                     /translation="MPTRRQLANAIRALSMDAVQKANSGHPGMPMGMADIAQVLWADH
                     LRHNPGNPLWPDRDRFLLSNGHGSMLLYSLLHLTGYPLSIEDIANFRQFGSHTAGHPE
                     VDQHLGIETTTGPLGQGLANAIGMALAEKLLAAEFNRPGHAIVDHHTYVFLGDGCLME
                     GISHEVCSLAGTLALGKLIAFYDDNGISIDGEVHGWFTDDTPKRFEAYGWHVVPNVDG
                     HDPEAVERAIQAAKAETARPSLVCCKTVIGWGSPNKAGTEATHGAALGEAEVAATREH
                     LGWTSPAFKIPAEIRAGWDAKAKGAKAEQAWDDRLAAYREAHPGLAAELERRLRGDLP
                     RDWRTKLQALVQANVEKPANVATRSSSQQVLNVLGAAIPEMLGGSADLTGSNNTNHKG
                     SKPITGHGGGNYIHYGVREFGMAAIMNGIALHGGFVPYGGTFLVFSDYARNAVRMSAL
                     MRRRVIYVFTHDSIGLGEDGPTHQPVEHVASLRMIPNLRLWRPCDTVETAVAWGDAVE
                     HTTGPTALVLTRQALAPATRTPEQIEAIRRGGYVLVDCEGAPDLVLIATGSEVSIAIE
                     AAKQMGPRRVRVVSMPCTSLFDAQDAAWRASVLPPGVPKVAVEAGVRDGWYRYVGTEG
                     AVVGMDTFGASAPAKKLFEHFGFTAAHVAEVAGGLLARA"
     gene            5382..6629
                     /gene="metK"
                     /locus_tag="VD737_05935"
     CDS             5382..6629
                     /gene="metK"
                     /locus_tag="VD737_05935"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HXW10132.1"
                     /translation="MSTSYLFTSESVSEGHPDKVADQISDAVLDAMLAQDKRARVACE
                     TLVKTGVAVVAGEVTTEAWVDLEELVRGVITDIGYTSSHVGFDGRTCGVINIIGKQSP
                     DIAMGVDRGNPEEQGAGDQGLMFGYASNETETLMPAPICYAHKLVERQAEVRKSKLLP
                     WLRPDAKSQVTCRYVDGKVVGLDAVVLSTQHDPEVKLEDLREAVMELIIKHVLPKEWL
                     AGTTKFHINPTGSFVIGGPVGDCGLTGRKIIVDSYGGMARHGGGAFSGKDPSKVDRSA
                     AYAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSITVETFGTGKVGQDRLEQLVRAH
                     FDLRPYGIIRMLDLVHPMYRSTAAYGHFGREPTHMTYKWKETIGGKRVEKSEAFTAFT
                     WEKTDKAEALRQDAGLVKRMAVG"
     gene            6703..7518
                     /locus_tag="VD737_05940"
     CDS             6703..7518
                     /locus_tag="VD737_05940"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXW10133.1"
                     /translation="MTSLGPIPLPLVLLVAGLAVAALVARLLAGALPGPRWATAGTFI
                     DMLLVGLIAGRLAFVLQWWPQYAADPWAIVRAGDGGHALWIAIPAGLAFGAWRVRKRP
                     ELRRPVAWGSAAGLVAWAILAAAVALMQRSVVHLPDVELTRLEGETVRLTGLSQGRPM
                     VVNLWATWCPPCRREMPVLAQAQARHHGIIFVFVNQGEGAPHVDQFLRGGALALRNVV
                     LDPASSVAQATGARGLPTTLFFDAGGRLADVHMGELTRASLEQKLRRLQPTYP"
     gene            complement(7568..8008)
                     /locus_tag="VD737_05945"
     CDS             complement(7568..8008)
                     /locus_tag="VD737_05945"
                     /inference="COORDINATES: protein motif:HMM:NF016184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur transferase domain-containing protein"
                     /protein_id="HXW10134.1"
                     /translation="MTAPWQYAEGHYAGGQPSAEQLGDLARDGVRTVINLRGPQERAD
                     YDEAGEAGRLGLRYVSMPITGATDLDRARVREFGRVLDDARREGGILIHCASANRVGA
                     MVALDEALNRDRPLGEALERGRAAGLSALEPAVVALAEREGARR"
     gene            complement(8048..8833)
                     /locus_tag="VD737_05950"
     CDS             complement(8048..8833)
                     /locus_tag="VD737_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXW10135.1"
                     /translation="MTDLQLMLGVLAGNAVGFTLGLVGGGGSILAVPLMVYLVGVRDP
                     HVAIGTSALAVAANALIGLTNHARKGNVKWRCAATFATAGVAGAWLGAVLGKAMDGQK
                     LLALFAVLMLVVAGLMVRSRGREGDPAVVLNRGNAPKLLGSGVATGLLSGFFGIGGGF
                     LIVPGLMASTGMPILFAVGSSLVAVTAFGLTTALSYAASGLVQGSLAVAFIAGGALGS
                     LFGARLATRLAGRKGTLDLALAGLIAVVALYMLFRTAGDLGWI"
     gene            complement(8915..9604)
                     /locus_tag="VD737_05955"
     CDS             complement(8915..9604)
                     /locus_tag="VD737_05955"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HXW10136.1"
                     /translation="MLKLYGFPVSNYVNMVHFALLEKGIPFEYVLTFPDQTPEFLAIS
                     PRGKVPVLETPQGYLNEASVILEYLEETGTGRPLLPSEPYARGYVRSLIKEIELYVEL
                     PGRSCFAEAFFGGRVSQSIKDKARDDLAGGFAAVRRHAKFAPFVAGAEFTLADVVFLY
                     SADLANTVAKVLFDTDHLAAWPEASALMERLGQNPHVQSIARRREAGRAAFVESVKAR
                     LAAAAAMVQGA"
     regulatory      9701..9777
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            9865..11283
                     /gene="ahcY"
                     /locus_tag="VD737_05960"
     CDS             9865..11283
                     /gene="ahcY"
                     /locus_tag="VD737_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011266417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HXW10137.1"
                     /translation="MNAVVTPNATQDYRVADMGLAEWGRKEMVIAESEMPGLMAVRRE
                     YGQEKPLKGARIAGSLHMTIQTAMLIDTLKALGADVRWASCNIYSTQDHAAAYVASTG
                     TPVFAYKGESLEEYWEFTHRIFEWPSGQFANMILDDGGDATLLLMLGARAEQDASVIA
                     KPANEEETALYAAIRRRLDSQPGWYSKRLAAIQGVTEETTTGVKRLYEFAKKGALPFP
                     AINVNDSVTKSKFDNLYGCRESLVDAIKRATDVMIAGKVAVVAGYGDVGKGSAESLRA
                     LSAQVWITEIDPICALQAAMEGYKVVTMDEACDKADIFVTATGNVNVITHEHMKRMKN
                     QAIVCNIGHFDSEIEIAALKQYAWENIKPQVDHVIFPDGKRLIILAQGRLVNLGCATG
                     HPSFVMSNSFTNQVLAQIELFKHGARYGKQVYVLPKHLDEKVAQLHLERIGVKLTTLT
                     QEQADYIGVPKHGPFKPEHYKY"
     gene            complement(11399..>11923)
                     /locus_tag="VD737_05965"
     CDS             complement(11399..>11923)
                     /locus_tag="VD737_05965"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXW10138.1"
                     /translation="LREIDGHLLEYLGDGLDAVTRAKPVAISPTIRFDEEPVRIRLPE
                     KVGTYARLGCAADPDPACPSAPARNQSHCTGEIVHADLTWAPGPPPYTVQPRYPYEAR
                     QLGISGTVAVAAHVDSSGLLCSGVVVRIPAGNAGPLADAALDAIQYWQLDPAMRDGRP
                     VESLRLLAVNFVLD"
BASE COUNT         1896 a         4140 c         4021 g         1866 t
ORIGIN      
        1 gggaccgggc tgtggatcat cgactccatc atctgggtcg ccgtgatcgt gacgcgatcg
       61 tatttacgcg cgacctgcag gatgtgcttc tgcaggccgg tgagttcggc gtagcccacc
      121 tcgatgccga ggtcgccccg cgcgaccatg acggcatcgc tcgcctgcac gatctcgtcg
      181 agcctcggaa tggcctcgct gcgctcgatc ttggcgacga tgcgcgcgtc gctgcccgcc
      241 tgccgcaaca gctcgcgcgc ctcctcgacg tcggcggcct ggcgcacgaa cgagacggcc
      301 aggtaatcga tgtcgagctc cgcgccgagc acgacgtctt cgcggtcctt gccggtcagc
      361 gccttggccg acagcccgcc gccgcggcgg ttgatgccct tgtggtcgcc gacttcgccg
      421 ccggtgagca cgcgacagac gacacggcga ccctcgacgg cggtgaccgt gagctcgatc
      481 tggccgtcgc ccaggatcag ctcgtcgccc ggtcgcacgt cctgcgccag ctcggcatac
      541 gtgatgccga cgccgcggtc ggtgccgtcg tgcgcatcga ggtccgggtc gagcacgaac
      601 gtggcgccct cgctcagcac cgtgcggccg ccggcgaacc gctcgatgcg gatcttcggt
      661 ccctgcaggt cgccgagcat cgcgacgtgc ttgcccacgc tggcagccgc ctcgcgcgcg
      721 cgctggacgc ggcggcggtg atcgccgggt tcgccgtgcg agaaattgac gcgcaggacg
      781 tcggcgcctt gccggatcac gtcctcgagg atgcccggcc cgtcggtggc ggggccgagc
      841 gtcgcgacga tcttggtgcg ccgactcagc atgagcacgg atctcctgca gcgatgagtt
      901 tctctcccgc ggacggtcgt cccgacgggc cttcgcagtg gctggctcag ccggcggcgc
      961 gctgctcgag catttcgacg gccggcagct tcttgccctc gaggaactcg aggaaggcgc
     1021 caccggcggt ggagatgtac gagatgtcct cggcgacgcc gtacttttcg atcgcggcga
     1081 tcgtgtcgcc gccgcccgcg atcgagaacg ccgggctccg cgcgacggcc tgcgcgatcg
     1141 tgcgcgtgcc ctcgccgaac tggtcgaact cgaacacgcc caccggaccg ttccacacga
     1201 tcgtgccggc gtcctgcagc gcggcggcga agcgctcggc cgtgtcgggg ccgatgtcga
     1261 ggatcatctc gttcggtgcc acctgcgcga ccggcttcac gtccgcctcg gcggaagccg
     1321 cgaactcccg ggcgaccacg acgtcggtcg gcagcgggat ctcgacgcct ccgcccttgg
     1381 aacgctcgag cagcgtgcgt gccgtgtcga tcatttcggc ttcgtgcagc gactttccga
     1441 tgggatggcc ctgggccgcc aggaacgtgt tggcgatgcc accgccgacg atcagcttgt
     1501 ccaccttgcc gagcaggttc tcgagcaccg tcagcttggt cgagactttc gagccggcga
     1561 cgatcgcgac cagcggccgt cgcgggctcg acagcgcctg ctcgagcgcg tcgagctcgt
     1621 tggtgagcag cggtcccgcg caggcgaccg gcgcgaactt gccgacgccg tgcgtgctcg
     1681 cctcggcgcg gtgcgccgtc gcgaacgcat ccatcacgta gatgtcgcag agcgcggcca
     1741 tcttctgcgc gagctcgtcc ttgttcttct tctcgccctt gttgaaccgg acgttctcga
     1801 gcagcacgac ctcgccgggc tcgctggtga cgccgttctg cagccagtcg cgctcgaagc
     1861 gcaccttgcg gcccagcagt tccgagagtc gcgcggcgac cggggccagc gagaactcct
     1921 cgttcgggac gccctcctcc gggcgtccca ggtgcgacat cagcatgacc cgcgccttcg
     1981 ccttcatcgc cgcttcgatc gtcggcagcg aggcgcggat gcgcgcgtcg ctcgtgacga
     2041 ccccgttctg gaccgggacg ttgaggtcct cgcggatgag cacgcgcttg ccgccgaggt
     2101 ccagttcgtt catgcgaagg acacggatca tgctcgctcc tgttcggcgt gacgtcgagc
     2161 gtgtcgcgac ggggatcact tcgcgatcac gcgcgccatt tcgagcacct tgctcgagta
     2221 gccccactcg ttgtcgtacc acgccacgac cttgacgaag gtgtcgtcga gcgcgatgcc
     2281 ggcttcggcg tcgaacaccg aggtgcagct ctcgccacgg aagtccgtcg cgacgacctt
     2341 ctcctcggtg taaccgagca cgcccttcat cgcgccttcg gaagccgctt tcatcgtggc
     2401 gcagatctcg tcgtacttgg cgggcttcga cagctcgacc gtcagatcga ccaccgagac
     2461 gtcggaggtc ggcacgcgga acgacatgcc cgtgagcttc ttgttgagct ccgggatgac
     2521 cttgccgacg gccttcgccg cgccggtcga ggaagggatg atgttctcga ggatgccgcg
     2581 gccgccgcgc cagtccttgt tcgagggacc gtcgacgacc ttctgcgacg cggtcgcggc
     2641 gtggacggtc gtcatcagcc cgcgcttgat gccccaggtg tcgtgcagca ccttggcgac
     2701 cggcgcgagg cagttggtcg tgcacgaggc gttcgagatg atcgcctcgc ccgcgtactt
     2761 cgagtggttg acgccgtaga cgaacatcgg cgtgtcgtcc ttcgacggcg ccgacaggat
     2821 gaccttcttc gccccggcgg ccaggtgctt ctccgccgtc tccttggtca ggaacagacc
     2881 ggtcgactcg accacgacgt cggcgccgag gtcgccccac ttgagctcgg ccgggtcctt
     2941 caccgcggtc aggcggatcc ggcggccgtt cacgaccagc gtgttgccgt cgaccgacac
     3001 gtcgcccttg aagcgcccgt gcaccgagtc gtacctcagc atgtacgcga gatagtccgg
     3061 ctcgagcagg tcgttgatgc ccaccacctc gacgtcggcg aagtccttga ccgcggcgcg
     3121 gaacaccatg cgaccgatgc gaccgaaacc gttgatgccg accttgatag ccatttacgt
     3181 ctctctccgt cagtggatct ttcccgaaac cgcggccgcc gcgtcaggca cgcgcgagca
     3241 gcccgcccgc gacctcggcc acgtgcgccg cggtaaagcc gaaatgctcg aacagcttct
     3301 tcgccggcgc cgacgcgccg aacgtgtcca tgccgacgac ggcgccttcg gtgccgacgt
     3361 agcggtacca gccgtcgcgc acgcccgcct cgaccgcgac cttgggcacg cccggcggca
     3421 ggaccgaggc gcgccaggcc gcatcctgcg cgtcgaacag gctcgtgcag ggcatcgaga
     3481 cgacgcggac gcgccgcggg cccatctgct tcgccgcctc gatcgcgatc gacacttccg
     3541 agccggttgc gatcagcacg aggtcaggcg caccttcgca gtcgaccagc acgtagccgc
     3601 cgcgccggat cgcctcgatc tgttccggcg tgcgcgtggc cggggcgagc gcctggcgcg
     3661 tcagcacgag ggccgtgggt ccggtcgtgt gctcgaccgc atcaccccag gccacggccg
     3721 tctcgacggt gtcgcaggga cgccagagcc gcaggttcgg aatcatgcgc aggctcgcga
     3781 cgtgctcgac gggctggtgc gtcgggccgt cctcgcccaa gccgatcgaa tcgtgcgtga
     3841 agacgtagat cacgcgccgg cgcatcagcg ccgacatgcg cacggcgttg cgtgcgtagt
     3901 ccgagaacac caggaacgtg ccgccgtacg gcacgaagcc gccgtgcagc gcgatgccgt
     3961 tcatgatcgc ggccatgccg aactcgcgca cgccgtagtg gatgtagttg ccgccgccgt
     4021 ggcccgtgat cggtttcgag cccttgtggt tggtgttgtt cgagccggtc aggtcggccg
     4081 agcctcccag catctcggga atcgccgcac cgagcacgtt gagcacctgc tgcgacgacg
     4141 agcgggtcgc gacgttggca ggcttctcga cgttggcctg gacgagcgcc tgcagtttcg
     4201 tgcgccagtc ccggggcagg tcgccgcgga gccggcgctc gagttcggcc gcgagcccag
     4261 ggtgtgcctc gcgatacgcg gcaaggcggt cgtcccaggc ctgttccgcc ttcgcgccct
     4321 tggccttcgc gtcccagccg gcgcggatct cggcgggaat cttgaaggcg ggcgaggtcc
     4381 agccgaggtg ctcgcgcgtc gcggccactt ccgcttcgcc gagcgccgcg ccgtgggtcg
     4441 cctccgtgcc cgccttgttg ggcgagcccc agccgatcac cgtcttgcag cagaccagcg
     4501 atggacgcgc cgtctcggct ttcgccgcct ggatcgcgcg ctcgacggcc tcggggtcgt
     4561 gaccgtcgac gttcggcacc acgtgccacc cgtacgcttc gaaacgcttc ggcgtgtcgt
     4621 ccgtgaacca gccgtgcacc tcgccgtcga tcgagatgcc gttgtcgtcg tagaacgcga
     4681 tcagcttgcc gagcgcgagc gtgcccgcca gcgaacacac ctcgtgcgaa atgccttcca
     4741 tcaggcaacc gtcgccgagg aaaacgtacg tgtggtggtc gacgatcgcg tgcccgggac
     4801 gattgaactc ggccgccagc agcttctcgg cgagtgccat gccgatcgcg ttggcgagcc
     4861 cctgcccgag cggacccgtg gtcgtctcga tgccgaggtg ctggtcgact tccgggtggc
     4921 cggccgtgtg cgagccgaac tggcgaaaat tcgcgatgtc ctcgatcgac agcggataac
     4981 cggtcaggtg cagcagcgag tacagcagca tcgagccgtg gccgttcgac agcaggaagc
     5041 ggtcgcggtc cggccacagc gggttgcccg ggttgtgcct caagtggtcc gcccacagca
     5101 cctgcgcgat gtcggccatg cccatcggca tgcccggatg gcccgaattg gccttctgga
     5161 ccgcatccat gctcaacgcc cggatggcgt tggcgagttg ccggcgggtg ggcatgaggg
     5221 accctcgtgg aaagcccggc attttagcgg gcgggcgtga cgcgggtcac ctttggtggg
     5281 tccggtccgc gtccgagggc atcaggacag accgaaacgg acgcgcgacg cacgatgccg
     5341 gcggctatac tccgcggcct gatcgccggg gaatctcata gatgtcaacc agttacctgt
     5401 tcacttccga gtccgtgtcc gagggtcacc cggacaaggt cgccgaccag atctccgacg
     5461 cagtgctcga cgccatgctc gcgcaggaca agcgggcgcg cgtcgcctgc gagacgctcg
     5521 tcaagaccgg cgtcgccgtc gtggccggcg aagtgaccac cgaagcgtgg gtcgacctcg
     5581 aggaactcgt ccgcggcgtc atcaccgaca tcggctacac ctcctcgcac gtcggcttcg
     5641 acgggcgcac ctgcggcgtc atcaacatca tcggcaagca gtcgccggac atcgcgatgg
     5701 gcgtcgaccg tggcaacccc gaggaacagg gcgccggcga ccagggcctg atgttcgggt
     5761 atgcgagcaa cgagaccgag acgctgatgc cggcgccgat ctgctacgcg cacaagctgg
     5821 tcgagcggca ggccgaggtc cgcaagagca agctgctgcc ctggctgcga cccgacgcca
     5881 agtcgcaggt cacctgccgc tacgtcgacg gcaaggtcgt gggcctcgac gcggtggtgc
     5941 tgtccacgca gcacgacccg gaggtcaagc tcgaggacct gcgcgaggcg gtcatggagc
     6001 tgatcatcaa gcacgtgttg cccaaggaat ggctcgccgg gaccaccaag ttccacatca
     6061 acccgaccgg cagcttcgtg atcggcgggc cggtcggcga ctgcggcctg accggtcgca
     6121 agatcatcgt cgacagctac ggcggcatgg cgcgccacgg cggcggcgcg ttctccggca
     6181 aggacccgtc caaagtcgac cgctcggccg cgtacgcggc ccgctacgtt gccaagaaca
     6241 tcgtcgcggc cggcctcgcc gaccgctgcg agatccaggt gtcgtacgcg atcggcgtcg
     6301 cggaaccgac gtcgatcacc gtcgagacct tcggcacggg caaggtcggg caggaccgcc
     6361 tcgagcagct cgtgcgcgcg cacttcgacc tgcgcccgta cggcatcatc aggatgctcg
     6421 acctcgtgca cccgatgtac cgctcgaccg cggcgtacgg ccacttcggc cgcgagccga
     6481 cgcacatgac ctacaagtgg aaggaaacga tcggcggcaa gcgcgtcgag aagagcgagg
     6541 cgttcacggc gttcacctgg gagaagacgg acaaggccga ggcgctgcgg caggacgccg
     6601 ggctggtcaa gcggatggcg gtgggctgac gtcgcctgac attgcgccgc acctcgccgg
     6661 ggatcgcgcc gccgattcac tggcgcggcc acgcgcgacg cgatgaccag cctcgggccg
     6721 atcccgttgc cgctcgtcct gctggtggcg ggactcgcgg tcgccgcgct cgtcgcgcgt
     6781 ctgctggcgg gcgcgctgcc cggcccccgc tgggccaccg ccggcacctt catcgacatg
     6841 ctgctcgtgg ggctgatcgc ggggcgcctc gcgttcgtgc tgcagtggtg gccgcaatac
     6901 gccgccgatc cctgggccat cgtccgcgcg ggcgacggcg gtcatgcgct ctggatcgcg
     6961 attcccgcag gcctggcgtt tggcgcatgg cgcgtgcgaa agcgaccgga gcttcggcgt
     7021 ccggtggcat ggggcagcgc cgcggggctc gtcgcgtggg caatcctcgc cgccgcggtc
     7081 gcgctgatgc agcgctcggt cgtgcatctg cccgacgtcg agctcacgcg tctcgaaggc
     7141 gaaacggtgc gcctgacggg gctaagccag ggacgaccca tggtcgtgaa tctctgggcg
     7201 acgtggtgcc cgccgtgccg gcgcgagatg cccgtgctgg cccaagccca ggctcgccat
     7261 cacggcatca tcttcgtctt cgtgaaccag ggcgagggcg cgccccacgt ggatcaattc
     7321 ctgcgtggcg gcgcgctggc gctgcgcaac gtcgtgctcg atcccgcgtc gagcgtcgcg
     7381 caggcgaccg gcgcgcgcgg cctgcccacg acattgttct tcgacgcagg gggccggctg
     7441 gcggacgtcc acatgggtga gctcacccgc gccagcctcg agcagaagct gcggcgcctg
     7501 cagccgacct atccctgagg gatttcggcc acctgggggc aatggcggaa aaccatcgcg
     7561 gacgtcttca acgtcgcgct ccctcgcgct cggcgagcgc gaccaccgcc ggctcgagcg
     7621 ccgacaggcc ggcggcgcgg ccgcgctcga gcgcctcgcc cagcgggcga tcacgattga
     7681 gcgcctcgtc cagcgcgacc atggcgccga ctcgattggc gctggcgcag tgaatcagta
     7741 tgccgccctc gcggcgcgca tcgtcgagca cgcggccgaa ctcccgcacg cgtgcgcgat
     7801 cgaggtcggt cgcgccggtg atcggcatcg acacgtaacg cagaccgagc cggccggctt
     7861 cgcccgcctc gtcatagtcc gcgcgttcct gcgggccacg caggttgatg accgtacgca
     7921 cgccgtcgcg cgcgaggtcg ccgagttgct cggccgaagg ctgtccgccg gcgtagtggc
     7981 cttcggcata ttgccagggt gcggtcactg gaaacctcca cgagtggctg gactgcgagc
     8041 gcgctggtca gatccagccg aggtcgcccg cggtgcgaaa gagcatgtag agcgcaacga
     8101 cggcgatcaa cccggcgagc gcgaggtcga gggtgccctt gcggccggcc agtcgcgtcg
     8161 ccagtcgagc gccgaacagg ctgccgagcg cgccgcccgc gatgaacgcc acggcaagcg
     8221 acccttggac gaggccggaa gccgcgtagc tcagcgccgt ggtcagtcca aacgcggtca
     8281 cggcgaccag cgacgacccg acggcgaaca ggatcggcat gccggtcgac gccatgagcc
     8341 cgggcacgat caggaagccg ccaccgatgc cgaagaatcc cgacagcagg cccgtggcga
     8401 cgccggaacc cagcagcttc ggcgcattgc cacgattgag cacgacggcc ggatcgccct
     8461 cgcgcccgcg cgagcgaacc atcaaacccg cgaccaccag catcaggacc gcgaacagcg
     8521 ccagcagttt ctggccgtcc atcgccttgc cgagcaccgc accgagccac gcgcccgcca
     8581 cgcctgcggt cgcgaacgtc gcggcgcagc gccacttcac attgcctttg cgcgcgtgat
     8641 tggtcaggcc gatcaacgca ttcgccgcga ccgcgagcgc gctcgtgccg atcgccacgt
     8701 gcggatcgcg aacgccgacc aggtacacca tcaacggtac cgccaggatg gaccctcccc
     8761 cacccacgag tccgagcgtg aacccgaccg cgttgcccgc cagcacgccg agcattagct
     8821 gcagatccgt cacgtagcct ccgctggaac gcgaggaggg tggtcacttc gacatcgggc
     8881 tcgagcccaa ccagcggtgg cccgcgacgc gccgttaagc gccctgcacc atcgcggccg
     8941 ccgccgcgag ccgcgccttg acggactcga cgaaggccgc gcggcccgcc tcgcgcctgc
     9001 gggcgatcga ctgcacgtgc gggttctggc ccaggcgctc catcaaagcc gacgcctccg
     9061 gccacgcagc caggtgatcg gtgtcgaaca gcaccttggc gaccgtgttc gcgaggtccg
     9121 cgctgtacag gaacacgacg tccgccagcg tgaactcggc tccggcgacg aatggtgcga
     9181 acttcgcgtg gcgtcgcact gcggcaaagc cccccgcgag gtcgtcacgc gccttgtcct
     9241 tgatcgactg cgacacccgg cctccgaaga acgcctcggc aaagcacgag cgtcccggca
     9301 gctcgacgta cagctcgatt tccttgataa ggctgcgcac gtagccgcgc gcgtagggct
     9361 cgctcggcag cagcggcctg cccgtgcccg tctcctcgag gtactcgagg atcacgctgg
     9421 cctcgttgag gtatccctgc ggcgtctcga gcaccggcac cttgccgcgc gggctgatcg
     9481 ccaggaactc cggggtctgg tccgggaacg tcagcacgta ctcgaacggg atgcccttct
     9541 cgagcagcgc gaagtgcacc atgttgacgt agttgctgac cgggaatccg tacagcttga
     9601 gcacggtcgt tctcctgcgg ggggtcggaa cgcccgagcg taccgcaaac ccggcagctg
     9661 gacttggtct gccggctccg gattcgctag acttcgcggc ctgcctgagg agcgctgcaa
     9721 cgggagtctg agcccgccag gctcaggcat tgggtttccc aaccgcgctc gctgaaccac
     9781 gtgccagtcg ttcaggcgag ccagcgaaca tcccttcctg tcgcttgatc gaaccggcac
     9841 ggtccaacga gagaggatcg accgatgaac gccgttgtta caccgaatgc gacgcaggac
     9901 taccgcgtcg ccgacatggg gctcgccgag tggggccgca aggaaatggt catcgccgag
     9961 agcgagatgc ccggcctgat ggccgtccgc cgtgagtacg gccaggagaa gccgctcaag
    10021 ggcgcgcgca tcgccggctc gctgcacatg accatccaga ccgcgatgct gatcgacacg
    10081 ctgaaggcgc tcggggcgga cgtgcgctgg gcgtcgtgca acatctattc gacccaggac
    10141 cacgccgccg cctacgtcgc gtccaccggc acgccggtgt tcgcgtacaa gggcgagtcg
    10201 ctcgaggagt actgggagtt cacgcaccgg atcttcgagt ggccgagcgg ccagttcgcc
    10261 aacatgatcc tcgacgacgg cggcgacgcg acgctgctgc tgatgctggg tgcacgcgcg
    10321 gagcaggatg cctccgtgat cgccaagccg gccaacgagg aagagaccgc gctctacgcc
    10381 gcgatccgca ggcgcctcga ctcgcagccg ggctggtact cgaagcggct cgccgcgatc
    10441 cagggcgtca ccgaggagac gacgaccggc gtcaagcgcc tctacgagtt cgcgaagaag
    10501 ggcgccctgc cgttccccgc gatcaacgtc aacgactcgg tcaccaagtc gaagttcgac
    10561 aacctgtacg ggtgccgcga atcgctggtc gacgcgatca agcgcgcgac cgacgtcatg
    10621 atcgccggca aggtcgcggt cgtcgcgggc tacggcgacg tgggcaaggg gtcggcggag
    10681 tcgctgcgcg cgctgtcggc gcaggtgtgg atcaccgaga tcgacccgat ctgcgcgctg
    10741 caggccgcga tggaaggcta caaggtcgtc acgatggacg aggcctgtga caaggccgac
    10801 atcttcgtga ccgcgaccgg caacgtgaac gtgatcaccc acgagcacat gaagcgcatg
    10861 aagaaccagg cgatcgtgtg caacatcggg cacttcgaca gcgagatcga gatcgccgcg
    10921 ctcaagcagt acgcgtggga aaacatcaag ccccaggtcg atcacgtgat cttccccgac
    10981 ggcaagcgac tgatcatcct ggcccagggg cggctcgtga acctcggctg tgcgaccggc
    11041 cacccgtcgt tcgtgatgtc gaactcgttc acgaaccagg tgctcgcgca gatcgagctc
    11101 ttcaagcacg gtgccaggta cggcaagcag gtgtacgtgc tgcccaagca cctcgacgag
    11161 aaggtcgcgc agctgcacct cgaacgcatc ggcgtgaagc tgacgacgct gacgcaggag
    11221 caggccgact acatcggcgt gccgaagcac gggccgttca agccggagca ctacaaatac
    11281 tagtgtttag tggctagcgg ttagcggtta gtcagacacg ggacggccgc cgcggggcgg
    11341 ccgtttccgg gctggcgaga catccctcag cagtgcacgg cgatcgccga ggttcgaatc
    11401 agtcgagcac gaaattgact gccaacagcc gcagcgactc cacaggccgt ccatcgcgca
    11461 tcgccgggtc gagttgccag tactgaatcg catcgagcgc agcgtcggca agcggtccgg
    11521 cattgccggc agggattcgg acgaccacac cgctgcacaa caggccgctg gagtcgacgt
    11581 gcgccgcgac ggcaacggtg cccgaaatcc ccagctgcct cgcttcgtat ggatacctgg
    11641 gctggacggt gtatggcggc ggtccaggcg cccacgtgag gtcagcgtgg acgatctcgc
    11701 ccgtgcagtg actctggttg cgcgccggcg ccgagggaca ggccggatcc ggatctgcgg
    11761 cgcaacccag acgcgcatag gtgcccacct tctcgggcag ccggattcga acgggttcct
    11821 cgtcgaatcg aatcgtcggc gagattgcca cgggcttggc gcgggtcacc gcatcgagac
    11881 cgtcccctag gtattcgagg agatgaccgt cgatctcccg caa
//
LOCUS       DASPFR010000231         3468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_918523, whole genome shotgun sequence.
ACCESSION   DASPFR010000231 DASPFR010000000
VERSION     DASPFR010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918523"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="VD737_05970"
     CDS             <1..1294
                     /locus_tag="VD737_05970"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HXW10139.1"
                     /translation="TDEIKHSIRMGAGNADVCMVEIGGTVGDIESLPVLEAIRQLGVE
                     LGRNNVLYMHLTLIPYIATSGEIKTKPTQHSVKELRSIGIQPDLLLCRCKDPLPPEQR
                     RKIALFTNVEERAVFSAIDADDIYKIPSLLHEQKLDDIVCEKFGLDAPPADLREWNQV
                     VTAKAAPDLTINLAMVGKYVNLKDAYISLNEALTHAGLRTRTRVNVDFIEATEIEKHG
                     LGALAGMDAILVPGGFGERGIEGKIEAVRYARENGVPYLGICLGMQLAIIEYARNVAG
                     LAGAHSTEFNRASPHPVIALITEWQDQKGSVEARDEQSNLGGTMRLGAQEIRVSHGSL
                     AHAVYGADVIRERHRHRFEFNNTYLQRLMNAGLRFSGFSRDGLVEIVELPNHPFFVAS
                     QYHPEFRSTPRDGHPLFTGFIRAAREHRLGQMPAAAGQ"
     gene            1291..2121
                     /gene="kdsA"
                     /locus_tag="VD737_05975"
     CDS             1291..2121
                     /gene="kdsA"
                     /locus_tag="VD737_05975"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HXW10140.1"
                     /translation="MKLCGFEVGHDQPFFLIAGPCVVESEGLVLEIAGRMQELTTALG
                     IPYVFKASYDKANRSSKASFRGPGIEEGLRILSEVKRQLGLPVLTDVHEDTPMAEVAA
                     VVDVLQTPAFLCRQTNFILSASSQGKPVNIKKGQFLSPWEMKNVIDKARSTGNEQILA
                     CERGFSFGYNNLVSDMRSLAVMRDFAPVVFDATHSVQLPGGQGSASGGQREFVPVLSR
                     AAVAVGVAGLFMETHPDPARALSDGPNAWPLDRMRPLLEVLKELDVAVKQRPFEETTL
                     "
     gene            2118..3422
                     /gene="eno"
                     /locus_tag="VD737_05980"
     CDS             2118..3422
                     /gene="eno"
                     /locus_tag="VD737_05980"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019837270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HXW10141.1"
                     /translation="MTTPTIIDIKAREIMDSRGNPTVEADVYVEGGARGRAAVPSGAS
                     TGTREAVELRDGDSKRYLGKGVLQAVINANGELRNALIGRDVTDQAGLDRTMIELDAT
                     ETKSRLGANALLAISLAAAHAAANFNRQSLFRYLGGNGAKVMPVPMMNIVNGGAHADN
                     SVDIQEFMVLPVGATSFAEALRCGAEIFHALKKVLHGQGLATAVGDEGGFAPNLPSNE
                     AALETILQAVERAGYRPGRDVYLGLDVASSEFFKGGEYHLESEGRQFSPAQFVDYLEG
                     LVNRFPIITIEDGMAEGDWDGWATLTQRLGKRIQLVGDDLFVTNTKILREGIDKGIAN
                     SVLIKPNQIGTLTETLAAIEMAADAGYSAVVSHRSGETEDSTIADIAVATRATQIKTG
                     SLCRSDRVAKYNQLLRIEAELGNDGSYAGRDAFPVPLPTSDV"
BASE COUNT          611 a         1192 c         1162 g          503 t
ORIGIN      
        1 caccgacgag atcaagcact cgatccgcat gggcgccggc aacgccgacg tctgcatggt
       61 cgagatcggc ggcaccgtcg gcgacatcga gtcgctgccg gtcctcgagg cgatccgcca
      121 gctcggcgtc gaactcggcc gcaacaacgt gctgtacatg cacctgacgc tgatcccgta
      181 catcgcgacg tccggcgaga tcaagaccaa gccgacacag cactcggtca aggagctgcg
      241 ctcgatcgga atccagccgg acctgctgct ctgccgctgc aaggacccgc tgccgcccga
      301 gcagcgccgc aagatcgctc tgttcaccaa cgtcgaggag cgcgcggtgt tctcggcgat
      361 cgacgccgac gacatctaca agatcccgtc gctgctgcac gagcagaagc tcgacgacat
      421 cgtctgcgag aagttcggcc tcgacgcgcc accggccgac ctccgggagt ggaaccaggt
      481 cgtgaccgcg aaggcggcgc cggacctcac gatcaacctc gcgatggtcg gcaagtacgt
      541 caacctgaag gacgcctaca tctcgctcaa cgaggcgctg acgcacgcgg gcctgcggac
      601 gcgcacgcgc gtcaacgtcg acttcatcga ggcgaccgag atcgagaagc acggcctcgg
      661 cgcgctcgcc ggcatggacg cgattctggt gcccggcggc ttcggcgaac gcggcatcga
      721 gggcaagatc gaggccgtgc gctacgcgcg cgagaacggc gtcccgtacc tcggcatctg
      781 cctcggcatg cagctcgcga tcatcgagta cgcgcgcaac gtcgccgggc tcgccggcgc
      841 gcacagcacc gagttcaacc gcgcctcgcc gcacccggtg atcgccctga tcacggagtg
      901 gcaggaccag aaaggctcgg tcgaggcccg cgacgaacag tcgaacctgg gcggcacgat
      961 gcgtctcggc gcgcaggaga tccgcgtgtc gcacggctcg ctcgcgcacg cggtctacgg
     1021 cgccgacgtg attcgcgagc gacaccggca tcgcttcgag ttcaacaaca cctacctgca
     1081 gcggctcatg aacgccggcc tgcggttttc cgggttctcg cgcgacggac tggtggagat
     1141 cgtcgaactg ccgaaccacc cgttcttcgt cgcgagccag taccaccccg agttcaggtc
     1201 gacgccgcgt gacgggcacc cgctgttcac gggcttcatc cgcgcggcgc gcgagcaccg
     1261 cctcggccag atgccggcgg cggcgggcca atgaagctct gcggtttcga ggtcgggcac
     1321 gaccagccgt tcttcctgat cgcgggcccc tgcgtcgtcg agagcgaggg gctcgtgctc
     1381 gagatcgccg ggcgcatgca ggaactcacg acggcgctcg gcatcccgta cgtgttcaag
     1441 gccagctacg acaaggccaa ccggtcctcg aaggcgagct tccgtggtcc cggcatcgag
     1501 gagggcctgc ggatcctgtc cgaggtgaag cggcagctcg gcctgcccgt gctcaccgac
     1561 gtgcacgagg acacgccgat ggcggaggtc gccgcggtcg tcgacgtgct gcagacgccg
     1621 gcgttcctgt gccggcagac caacttcatc cttagcgcct cgagccaggg caagccggtc
     1681 aacatcaaga agggccagtt cctctcgccc tgggagatga agaacgtcat cgacaaggcg
     1741 cgttcgaccg gcaacgagca gatcctcgcc tgcgagcgcg gcttctcgtt cggctacaac
     1801 aacctcgtct ccgacatgcg ttcgctcgcc gtgatgcgcg acttcgcacc ggtggtgttc
     1861 gacgcgaccc actcggtgca gctgccgggc gggcagggga gcgcgtcggg cgggcagcgc
     1921 gagttcgtgc ccgtgctctc gcgcgcggcc gtcgcggtcg gcgtggcggg actcttcatg
     1981 gaaacgcacc ccgatcccgc gcgggcgctg tccgacggcc cgaacgcctg gccgctcgac
     2041 cgcatgcgcc cgctgctcga ggtgctcaag gaactggacg tggccgtgaa gcagcggccg
     2101 ttcgaggaaa ccacgctatg acgacgccga ccatcatcga catcaaggcc cgcgagatca
     2161 tggattcgcg tggcaacccg acggtcgagg ccgacgtcta cgtcgagggc ggcgcccgag
     2221 gccgcgcggc ggtgccgtcg ggggcttcga ccggcacgcg cgaagctgtc gaactgcgcg
     2281 acggcgactc gaagcgctac ctcggcaagg gcgtgctgca ggcggtcatc aacgcgaacg
     2341 gcgagttgcg caacgcgctg atcggccgcg acgtgaccga ccaggcgggc ctcgaccgca
     2401 cgatgatcga actcgacgcg accgagacca agtcgcggct cggcgcgaac gcgctgctcg
     2461 cgatatcgct ggccgcggcg cacgcggccg cgaacttcaa caggcagagc ctgttccgtt
     2521 acctcggcgg caacggtgcc aaggtgatgc cggtgccgat gatgaacatc gtcaacggcg
     2581 gggcgcacgc cgacaacagc gtcgacatcc aggaattcat ggtgctgccg gtcggtgcga
     2641 cgagtttcgc cgaggcgctg cgctgcggcg ccgagatctt ccacgcgctc aagaaggtcc
     2701 tgcacgggca gggcctcgcg acggcggtcg gtgacgaggg cggtttcgcg ccgaacctgc
     2761 cgtccaacga ggcggcgctc gagacgatcc tgcaggccgt cgagcgcgcg ggctaccggc
     2821 ccggccgtga cgtgtacctg gggctcgacg tcgccagctc ggagttcttc aagggcggcg
     2881 agtaccacct cgagtccgaa gggcgccagt tcagcccggc gcagttcgtc gactacctgg
     2941 agggactcgt caaccggttt ccgatcatca cgatcgagga cggcatggca gagggcgact
     3001 gggacggctg ggccacgctg acgcagcggc tcggcaagcg catccagttg gtcggtgacg
     3061 acctcttcgt caccaacacg aagatcctgc gcgaaggcat cgacaagggg atcgccaact
     3121 cggtactgat caagcccaac cagatcggca cgctgacgga gacgctcgcc gcgatcgaga
     3181 tggcggccga tgcgggctac agcgccgtcg tctcgcatcg ctcgggcgag accgaggaca
     3241 gcacgatcgc cgacattgcg gtcgcgacgc gggcgacgca gatcaagacc gggtcgctgt
     3301 gtcgctcgga ccgtgtcgcc aagtacaacc agttgctgcg catcgaggcg gagctcggca
     3361 acgacggctc gtacgccggg cgcgacgcct ttcccgtgcc gctgccaacc agcgacgtct
     3421 gagctttctc ccactagaca ctcaccacca gacactagac acttcgcc
//
LOCUS       DASPFR010000232         5630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_186650, whole genome shotgun sequence.
ACCESSION   DASPFR010000232 DASPFR010000000
VERSION     DASPFR010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186650"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1152)
                     /locus_tag="VD737_05985"
     CDS             complement(148..1152)
                     /locus_tag="VD737_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10142.1"
                     /translation="MRRRRTRDFEVFSMSFLDTICCAFGAIILLFMLSKFGEPQAFEK
                     SRLDLEGRLLALQEERAAIRGQTEILNRDLTERERQLSEIRLKLARLRGDLSNIRGQY
                     QSSEQDADVANKLEGQLVAAQQQLTEDMKRVLGAEFRRPAQDAVAGLPVDSQYIIFII
                     DTSGSMANYAWPLMLRKMQEVLDAYPTVKGWQVMSDEGTYMFPSYRGRWLPDTPNQRK
                     LVLDRLRDWFPFSNSSPVEGIVEAIRTYYSTGLRISLYVLGDEFTGSSIDSVVRAVDQ
                     INREGRGGRRVRIHALGFPVRPDAPQVTNIRFATLMRVLCARNGGTFVGLNEPSSYRR
                     "
     gene            complement(1162..2262)
                     /locus_tag="VD737_05990"
     CDS             complement(1162..2262)
                     /locus_tag="VD737_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXW10143.1"
                     /translation="MARRRRKFDIFSMSFLDTICCAFGAIVLLYMILNAAGSRAFQND
                     TSQLRAEVDKIEEEVLQGYADLVVLRNSLEKKETDAVRTEGLSDRVLEEKKRLEEQLA
                     EADKTTISRREAIERLKADLKQIEEERKRLPAGTPAEGKPGNRVKGFVGTGDRQYLSG
                     LKLGGERIVILVDVSASMLDETVVNIIRLRNMPESRRLRSEKWRRTVATVDWLTAQIP
                     SKSKFQVIAFNVKAWPVVPSSTGKWLDGDDAQALTDVVTHLRRTVPADGTSLENAFTL
                     MNSLTPAPDNVFLITDGLPTQGTAPPLVRKTIDGDGRLRLFERALAKFPNRVPANVIL
                     MPMEGDPAAPAAFWTLARRTNGAFLMPARDWP"
     gene            complement(2249..3688)
                     /locus_tag="VD737_05995"
     CDS             complement(2249..3688)
                     /locus_tag="VD737_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXW10144.1"
                     /translation="MVAARLVAIELNDAGVVAARWDAGEARPVLQGPPSPGFAVLHDG
                     RVLVGQEAFERHRLAPLYAQNRYWQALGVEPLPWSAAGVATAADLAHAHLSALLAPAI
                     AAGANEAVLAVAPGYTREQLGLLVGIANEAGATVRGLVDLGIAAAATVPPAPHMLHLD
                     LQLHQAAATLLESSRADGVLRRARYELLPGAGVIAFNQAIAGSIATTFVRETRFDPLH
                     EAMTEQRLHDRMPGWLAGLAEQDEVEAAIEAGGLTHQVTLQRSELIAAVAKPAADVLR
                     LVQGARPAGTMLHVCITPRVAAVPGLLANLTALRDCTVVMLEPGAAAVGALQAAAAIV
                     RAPDSISLVHRLPLPGATEALATTAVAGSDAVPAESVPTHVLFRGRAWPLTDVPLTLG
                     WSVGAAARALALPAGIAGVSRSHCALWLREGQAIVEDQSTYGTFVNDERVGGRVALRV
                     GDALRLGAPGVTLELIRVMESFPSHHGTA"
     gene            complement(3697..4494)
                     /locus_tag="VD737_06000"
     CDS             complement(3697..4494)
                     /locus_tag="VD737_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXW10145.1"
                     /translation="MKKLGTREFLFEVFSLILIAILVQAVYATYVRPRAEQIRLYDEA
                     QMKKDPNYVPARTLEVIIKDPEQEICFILALWSLAIMGYKVWTLRRGQRLLQMDLLHL
                     PEGMKILPEDSRDYARQVEALPQEMRRELLPRALLSGLHRFGSTRNIQDVASAIRDTC
                     ELEFGRLEAELSMVRYVAWAIPAIGFVGTVRGIGESLQQAHKAVEGDVSGVVAGLGIA
                     FNSTLVALALSIFVMFLLHQVQLRQERAVLDTEEFLDTRVVRQMLVK"
     gene            complement(4509..4859)
                     /locus_tag="VD737_06005"
     CDS             complement(4509..4859)
                     /locus_tag="VD737_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10146.1"
                     /translation="MTRHRPVAAWLATLAVAAAGAATLALAQQPSPPSTPPPPAASDS
                     QPTPAPSPPTDASTTEQDATEQDATEPGATEPSDKPPAAATPKPATAKGSPQRFEPTE
                     KVRPDFDVAFPIDI"
     gene            complement(4864..>5630)
                     /locus_tag="VD737_06010"
     CDS             complement(4864..>5630)
                     /locus_tag="VD737_06010"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HXW10147.1"
                     /translation="TMGSPRRESGRRSNEAQRPVELRRRYYLGTREVTNGEYKQFRPQ
                     HRSGFVQQNTLELDRQPVVNVSWQEAVEYANWLSQQEGLPAFYQLQGGKYVPAQPANT
                     GYRLPTEAEWEWAARANRDGSLRKYPWGDALPVPAGSGNYGDRKAQPLLQTILAELDD
                     GHVVTANAGSFAANALGFHDMGGNVAEWTTDLYTVQPPAATVAVDPLAAAPGNVHVIR
                     GSSWRHATVTELRLAFRDYGEGRRDDLGFRIARYAE"
BASE COUNT          806 a         1968 c         1963 g          893 t
ORIGIN      
        1 cgacgaccag catcagcgcg gcgcacgccc gggcccaccc gccgcggaac gacctcctcg
       61 cggcaccggt ggccaggcgt gacgcgccgg caggtcggtc gattcgttgc atgcttgcgt
      121 tcatcgtgtc gtttcctgtc cgccgattca gcggcgatag ctcgacggct cgttgaggcc
      181 gacgaacgtg ccgccgttgc gcgcgcacag cacgcgcatc agcgtcgcga accggatgtt
      241 ggtgacctgc ggcgcgtcgg gccgcaccgg gaacccgagc gcgtggatac ggacgcgccg
      301 tccgccgcgc ccctcgcggt tgatctggtc gacggcgcgc accacggagt cgatcgagga
      361 gcccgtgaac tcgtcgccga gcacgtagag gctgatgcgc agtcccgtgc tgtagtaggt
      421 gcggatcgcc tcgacgatgc cttcgaccgg gctcgagttg ctgaacggga accagtcgcg
      481 cagccggtcc agcaccagct tgcgctggtt cggcgtgtcc ggcagccagc gcccgcgata
      541 cgacgggaac atgtacgtgc cctcgtcgct catgacctgc caacccttca ccgtgggata
      601 tgcgtcgagc acttcctgca tcttgcgcag catcagcggc cacgcgtagt tggccatcga
      661 gccggaggtg tcgatgatga agatgatgta ctggctgtcc accggcaggc ccgcgacggc
      721 gtcctgggcg gggcggcgaa actcggcgcc cagcacgcgc ttcatgtcct cggtgagctg
      781 ctgctgcgcg gccacgagct ggccctcgag cttgttggcg acgtcggcgt cctgctcgct
      841 cgactggtac tggccgcgga tgttgctcag gtcgccgcgc agccgtgcga gcttgagccg
      901 gatctcggac agctgccgct cgcgttcggt caggtcgcgg ttcaggatct ccgtctggcc
      961 gcggatcgcc gcacgctcct cctgcagcgc cagcagccgg ccctcgaggt cgagccgcga
     1021 cttctcgaac gcctgcggct cgccgaactt gctcagcatg aacagcagga tgatcgcgcc
     1081 gaacgcgcag cagatcgtgt cgaggaacga catgctgaag acttcgaagt cgcgcgtacg
     1141 ccggcgtcgc atgcggggct cctacggcca gtcccgggcg ggcatcagga aggcaccgtt
     1201 ggtgcgccgc gccagcgtcc agaacgcagc cggcgcggcg ggatcgcctt ccatcggcat
     1261 caggatcacg ttggcgggca cgcggttcgg gaacttggcg agagcgcgtt cgaacaggcg
     1321 cagccgcccg tcgccgtcga tggtcttgcg cacgagcggc ggcgcggtgc cctgcgtcgg
     1381 caacccgtcg gtgatcagga acacgttgtc aggcgccggc gtcagcgagt tcatcagcgt
     1441 gaacgcgttc tcgaggctgg tgccgtcggc cggcaccgta cggcgcaggt gcgtgacgac
     1501 gtcggtcaat gcctgcgcgt catcgccgtc gagccacttg cccgtgctgc tcggcaccac
     1561 gggccaggcc ttcacgttga acgcgatcac ctggaacttg gacttcgagg ggatctgagc
     1621 cgtgagccag tcgaccgtcg ccacggtgcg ccgccatttc tccgaccgca gccgccgcga
     1681 ctcgggcatg ttgcgcagcc ggatgatgtt gacgaccgtc tcgtcgagca tgctcgcgga
     1741 gacgtcgacc aggatcacga tgcgctcgcc gccgagcttg aggccgctca ggtactggcg
     1801 gtcgccggtg cccacgaaac ctttcacgcg attgccgggc ttgccctcgg ccggcgtgcc
     1861 tgccggcagg cgcttgcgct cctcctcgat ctgcttgagg tccgccttga gcctctcgat
     1921 ggcttcgcga cgcgagatcg tggtcttgtc ggcttccgcg agctgctcct cgagccgttt
     1981 cttctcctcg agcacgcggt ccgagagccc ttcggtacgc acggcgtccg tttccttctt
     2041 ctccaggctg ttgcgcagca cgaccaggtc cgcgtagccc tgcagcacct cctcctcgat
     2101 cttgtcgacc tcggcgcgca gctgcgaagt gtcgttctgg aacgcgcggc tgcccgcggc
     2161 gttcaggatc atgtacagca gcacgatcgc gccgaaggca cagcagatcg tgtcgaggaa
     2221 cgacatgctg aagatgtcga acttgcgtct acgccgtgcc atggtgcgac gggaaactct
     2281 ccatgacccg gatcagctcg agcgtgacgc ccggggcgcc gagacgcagc gcgtcgccga
     2341 cgcgcagcgc gacccgaccg ccgacgcgtt cgtcgttgac gaacgtgccg tacgtgctct
     2401 ggtcctcgac gatcgcctga ccctccctga gccacagcgc gcagtgactg cgtgacacgc
     2461 cggcgattcc cgccggcagg gccagcgccc gcgcggcggc gccgaccgac cagccgagcg
     2521 tcaacggcac gtcggtgagc ggccaggccc ggccgcggaa cagcacgtgc gtgggcaccg
     2581 attctgcggg caccgcatcc gaacccgcga ccgcggtcgt ggcgagcgcc tcggtcgcgc
     2641 cgggcagtgg cagccgatgc acgagcgaga tcgagtccgg ggcccgcacg atcgccgcag
     2701 cggcctgcag cgcgccgacc gcggccgccc ccggctcgag catcacgacc gtgcagtcgc
     2761 gcagcgcggt caggttcgcg agcagccccg gcaccgccgc gacgcgcggc gtgatgcaga
     2821 cgtgcagcat cgtgcccgcg ggtcgcgcgc cctggaccag ccgcagcacg tcggcagcgg
     2881 gtttcgcgac ggccgcgatc agttccgacc gctgcagggt cacctggtgc gtgaggcctc
     2941 cggcctcgat cgccgcctcc acctcgtcct gctccgcgag tcccgcgagc cagccgggca
     3001 tccggtcgtg caggcgctgc tccgtcatcg cttcgtgcaa ggggtcgaaa cgagtctctc
     3061 gcacgaacgt cgtcgcgatc gacccggcga tcgcctggtt gaacgcgatc acgcccgcgc
     3121 ccggcagcag ctcgtaccgg gcgcggcgca gcacgccgtc cgcgcgggac gactcgagca
     3181 gcgtcgcggc cgcctggtgc agctgcaggt cgaggtgcag catgtgcggt gcgggcggca
     3241 cggtggcggc cgccgcgatc cccaggtcga cgagcccgcg cacggtggcg ccggcctcgt
     3301 tggcgatgcc gaccagcagc ccgagctgct cccgcgtgta tcccggcgca acggcgagca
     3361 cggcctcgtt cgccccggcg gcgatggcgg gcgcgagcag cgccgacagg tgcgcgtgcg
     3421 cgaggtcggc cgccgtcgcg acgccagccg cgctccaggg cagcggctcg acgccgagcg
     3481 cctgccagta gcggttctgc gcatagagcg gcgcgagccg gtgacgctcg aacgcctcct
     3541 gcccgaccag cacgcggccg tcgtgcagca cggcgaagcc cgggctggga ggcccctgga
     3601 gcacgggtcg cgcctcgcca gcgtcccagc gcgccgcgac gacgccggcg tcgttgagct
     3661 cgattgccac gagacgggct gcgaccatcg cgcgacctac ttgaccagca tctggcgcac
     3721 gacgcgcgtg tcgaggaact cctcggtgtc gagcacggcg cgctcctgcc gcagctggac
     3781 ctggtgcagc aggaacatca cgaagatgct cagcgccagc gcgacgagcg tcgagttgaa
     3841 cgcgatgccg aggccggcga cgacgccgga aacatcgccc tcgacggcct tgtgcgcctg
     3901 ctgcagcgat tcgccgatgc cgcggaccgt gccgacgaag ccgatcgccg gaatggccca
     3961 cgccacgtag cggaccatcg acagctcggc ctcgagccgt ccgaactcga gctcgcaggt
     4021 gtcgcggatg gccgaggcca cgtcctggat gttgcgcgtc gagccgaacc gatgcagccc
     4081 gctcagcagc gcgcgcggca gcaactcgcg ccgcatctcc tgcggcagcg cctcgacctg
     4141 gcgcgcgtag tcgcgcgagt cctcgggcag gatcttcatg ccttcgggca ggtgcagcag
     4201 gtccatctgc agcaggcgtt gccctcgccg cagcgtccag accttgtaac ccatgatcgc
     4261 gagggaccag agcgcgagga tgaagcagat ctcctgctcg ggatccttga tgatgacctc
     4321 gagcgtgcgc gcaggcacgt aattcgggtc cttcttcatc tgcgcctcgt cgtacaggcg
     4381 gatctgctcg gcgcgcgggc gcacgtaggt cgcgtagacg gcctgcacga ggatcgcgat
     4441 caggatcagc gagaagactt cgaacaggaa ctcacgtgtg cccagttttt tcatgtcgta
     4501 aagcgcactc agatgtcgat cgggaacgcg acgtcgaagt ccggacggac cttttcggtc
     4561 ggctcgaaac gttgcggcga gcctttcgcg gtggcgggct tcggcgtcgc agcggcgggc
     4621 ggcttatcgc tcggctccgt cgcgcccggc tccgtcgcgt cttgctccgt cgcatcttgc
     4681 tccgtcgtgc tcgcgtccgt gggcggcgac ggggccggcg tgggttgcga gtcgctggcg
     4741 gccggcggag gcggcgtgga cgggggcgag ggctgttgtg cgagggccag cgtcgcggca
     4801 cccgcagcgg ccaccgcgag cgtcgcgagc caggccgcga ccgggcgatg tcgagtcatg
     4861 gcatcactcc gcatagcggg cgatccggaa gccgaggtcg tcgcgacgac cctcaccgta
     4921 gtcgcggaag gcgaggcgca gttcagtgac ggtcgcgtgt cgccagctcg agccgcggat
     4981 cacgtgcacg ttgccgggcg cggcggcgag cggatcgacc gcgacggtcg ccgcgggcgg
     5041 ctggacggtg tagaggtccg tcgtccattc cgcgacattg ccgcccatgt cgtggaagcc
     5101 gagcgcgttg gcggcgaacg atcctgcgtt cgcagtcacc acgtgtccgt cgtcgagctc
     5161 cgcgaggatc gtctgcagca gcggctgtgc cttgcgatcg ccgtagttgc ccgagcccgc
     5221 aggcaccggc agcgcatcgc cccacgggta cttgcgcagt gagccgtcgc ggttggcgcg
     5281 tgccgcccac tcccactcgg cctccgtcgg caaccgatac ccggtgttgg ccggctgcgc
     5341 cggcacgtac ttgccgccct gcagctgata gaacgcgggc agtccttcct gctggctcag
     5401 ccagttggcg tactcgaccg cttcctgcca gctcacgttg acgaccggct ggcgatcgag
     5461 ttcgagcgtg ttctgctgca cgaacccgga gcgatgctgt ggccggaact gcttgtattc
     5521 gccgttggtg acctcgcgcg tgccgaggta gtagcggcgg cgcagttcga cgggccgttg
     5581 cgcctcgttc gaccgccgac ccgattcgcg gcgcgggctg cccatcgtgt
//
LOCUS       DASPFR010000233         8525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1164350, whole genome shotgun sequence.
ACCESSION   DASPFR010000233 DASPFR010000000
VERSION     DASPFR010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8525
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164350"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VD737_06015"
     CDS             <1..489
                     /locus_tag="VD737_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10148.1"
                     /translation="RHLDERNRRHLAYAARTSKRLARTLFRTMARHGPKLEREQVLLG
                     NLVDVGVELFVMAATLSYADHLVATRPADRSPLDLADLHCLEARRRIETNFRAVRHNF
                     NRSYGKVADQLMNGQLDWLGEGAANPIPPQYRDWQRHDYEHPGVARTPVAAAERARRE
                     AA"
     gene            616..1140
                     /locus_tag="VD737_06020"
     CDS             616..1140
                     /locus_tag="VD737_06020"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HXW10149.1"
                     /translation="MPARSRGFPPIATPDARVLILGSLPGQVSLARRQYYAQPHNAFW
                     RIMGALFGAGLEQPYESRVERLKTKRVAVWDVCRAATRPGSLDSSIDVSSVIPNDFGT
                     FFRRHPAIAAVFTNGGTATRLYQRLVQPRLAGAPRELPLHALPSTSPAHASLRFEQKL
                     ERWRQVADVLDDIG"
     gene            1137..2126
                     /locus_tag="VD737_06025"
     CDS             1137..2126
                     /locus_tag="VD737_06025"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXW10150.1"
                     /translation="MIATAHALRWACVAIASSALAGCMPLTRAPASVPSHEVPADSVE
                     LYAVGDMADCRRDPPGQSAARLVSALVPRGATLLALGDLVYPYADSETLARCYEPTWG
                     SHRATTLAVAGNHDYVENGSRTFREYFDLDRRTVSERYVAYTERLSADWLLVVVDSNV
                     RGGALDRQLEWLRETLERVRPRSGAGASPASPRCLAVAWHAPLHSSGWHRGSGDHMRP
                     YWEIVDAYGADLVLSGHEHFYEAFDPFDADGRRVASGPRQFTVGTGGARLYGFWKPPY
                     QSRARVLEFGVLHLVLAPGRYLWRFVDLDRRVRDAGTADCRGGAGVPTPVDLR"
     gene            2191..3540
                     /locus_tag="VD737_06030"
     CDS             2191..3540
                     /locus_tag="VD737_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005252496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HXW10151.1"
                     /translation="MQAFTGWMTALNSFVWGPAMLVLILGTGFYLQLRLRGMPLLRIP
                     GGLRLVWGGRGGDSQHGEVSPFAALMTCLAATIGVGNIAGVATAVALGGPGAVFWMWM
                     TALVGMATKYAEVLLAVHYRERDADGRWVGGPMYAIKNGLGRRWAWLGTLFAVFGGIA
                     GFGIGNMVQANSIAAAMQRSFSVSPTVTGVVLTVLTGAVVLGGIKRIAAVANWLVPFM
                     AVGYVVAGLVVLVTHAAAVPQAFALIVETAFTGTAAVGGFTGAAMIAAIRYGVARGIF
                     SNEAGLGTAGIAQAAGMSNDPVRSGLIGMMGTFFDTIVVCSITGLAIVVSGAWDSGET
                     GAQLTQLAFQSAMPGIGDEVVAISLGVFALTTIFGWSYYGERCWQFLVGTWTIRPYRV
                     LWTVAVFFGAVTHLDLAWTIADTLNALMAVPNLIALLALSPVVVQLTRAGLADALRS"
     gene            3592..4035
                     /locus_tag="VD737_06035"
     CDS             3592..4035
                     /locus_tag="VD737_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10152.1"
                     /translation="MTVKLPLPYRRSRGLAPGNDLLGIPGVALDVVGLEIGVDLEAGW
                     RRRVEHAALAIGWPSPAFARRQTEQGHTLAYTAPADQLQTARTANEWAVCAALVELDP
                     CHWASLLENLPGSVPASARDAVGQDAAIDRLVRLGAAEAAAHRVS"
     gene            complement(4048..5814)
                     /locus_tag="VD737_06040"
     CDS             complement(4048..5814)
                     /locus_tag="VD737_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HXW10153.1"
                     /translation="MPSTKPRPARPGRTLALLLLAFGVASCRTDPTPDAAPAPAAPVT
                     GATVSLRAGVAAANPIAVDAGLEVLRAGGSAVDAAVAVQAALGLVEPQSSGLGGGAFM
                     LHYDAQTRAVTAFNGREAAPAGATPDMFLDEHGAPLSYADAVTSGRATGVPGAIAMLG
                     VAHERFGKRPWRELFDASIRAAEQGFVVPQRLGRFANSTFRQATLPDATALFSRPDGT
                     RVQAGDTLRNPAYAQTLRTLAARGPRALLEGPLAEQIVARTHDAPRPGALTLADMAAY
                     RPLVTPALCKPFRIYLVCVPPPPSSGVSLLELLGLLERTDIAQSGPDDPRAWFLFAEA
                     SRLMYADRDRYVADPEYVAVPVEGLLDVRYLDERARRIGPRAASDAPEPGTPPGASLT
                     RAGRDATLDVAGTSHFVVVDDEGDVVSMTTSVESLFGSGRAVGGFFLNNQLTDFSFTA
                     VDAEGHKVANAVAGGKRPRSSMAPVIVLARDDGDLVAALGSPGGSAILAYNAKALLGL
                     LAWDLPLQAAFDLPNVIARGTEVGGEVAKLAPDVVSGLAERGVTVKPGRGEESGLHGI
                     AVRGNVFEGAADRRREGVWREK"
     gene            5892..7262
                     /locus_tag="VD737_06045"
     CDS             5892..7262
                     /locus_tag="VD737_06045"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXW10154.1"
                     /translation="MRSVVPVTVRLSLALSVALGCSAAVAQPAPVDRPPSPGVAVAPR
                     PTVVVVHAGRLLDRPGRAPRGPSTLVVRDGRVSEVLDGFVDVPGAQRVHDQRSRFVLP
                     GLIDSHVHLDSDAGGQQARLEDVQLETARFAYQALANAHKTLRAGFTTVRNLGDRYGV
                     TLALRDAIRAGQVIGPNIVDAGRSISTTSGHMDYALGYREDFREVLHAHDNLCDGIES
                     CRRAVRTQVGRGVDVIKIATTGGVNSQVGAGLGQQMFADEARAIVETARLYGKKVAVH
                     AHGVDGIAVALASGVDSIEHGTMLDDATITTMARQGTWYVPTLSTVNGYIERLATNPD
                     AYSPEVKAKVEWRIEVTGQSLEKAVPRGVKIAFGTDAGVSRHGRNADEFELMVKHGMT
                     PAAAIEAATVNAAALLGLDDVGTLEPGKRADLIAVEGDPLADVTVLKRVPFVLKDGRE
                     FTGGAR"
     gene            7313..7924
                     /locus_tag="VD737_06050"
     CDS             7313..7924
                     /locus_tag="VD737_06050"
                     /inference="COORDINATES: protein motif:HMM:NF017855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HXW10155.1"
                     /translation="MQPLPYSDACERNKEPILAVLRRYLADTAGLVLEIGSGTGQHAV
                     HFARHLPQLTWQPTDQAEELATLNARVGFEGPCNLLPPLELDVADRRWPCDSDSVDAV
                     FSANTLHIMDWKHVQSFFRGVGRVLRPEGLLIVYGPFRYSGQYTSHSNHAFDDALRRR
                     DPASGIRDFEAVDALASAQALALVEDVAMPANNQTLIWRKSAA"
     gene            complement(7948..>8525)
                     /locus_tag="VD737_06055"
     CDS             complement(7948..>8525)
                     /locus_tag="VD737_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10156.1"
                     /translation="INLGDVGTHADVSVANGGSGDVSITEVRTSDPWLRVTPAAVDDH
                     GLGRYAIDAERSTLPSGSHGGWVDFVSSAGTERVTVALRVTASTLAPDAGLQYVLLMD
                     PTTRAATGAQTSIRVLGTATSYRIEDVAPGAYLVVSGTDMNNDGSICDDGEACGAYPV
                     EPEPTVVEVGGAAVDRIDFTTAFRTNVEADR"
BASE COUNT         1180 a         3034 c         3039 g         1272 t
ORIGIN      
        1 cggcatctcg acgagcgcaa ccggcgtcat ctggcgtacg cggcccggac gagcaagcgc
       61 ctcgcccgca cgctgttccg gaccatggcc cggcacggtc cgaaactcga gcgcgagcag
      121 gtcctgctcg gcaacctcgt cgacgtcggc gtcgagctct tcgtcatggc ggcgacgctg
      181 tcctatgccg accacctcgt ggccacgcgc cccgccgacc gctcgccgct cgacctggcg
      241 gacctgcact gcctcgaggc acgccgacgc atcgagacga atttccgcgc ggtgcggcac
      301 aacttcaacc gctcgtacgg caaggttgcg gatcagctca tgaacggcca gctggactgg
      361 ctcggcgagg gcgcggcgaa tccgattccg ccgcagtatc gggactggca acgccacgac
      421 tacgagcatc ccggcgtcgc gcgtacgccc gtggcagcag cggagcgggc ccggcgggag
      481 gccgcgtagc tcgcgacgac gcacctctcc cggctgcgcg ggagaggtcg tcacgcagtg
      541 ccgggtgagg gccaggctcg tgcttcgccc accgcgcgct ttgcgacgag cgcgtgggcg
      601 gtttaccttg ggcggatgcc cgcccgttcc cgaggtttcc cgcccattgc gacccccgac
      661 gcgcgggtgc tgatcctcgg ttcgctgccg ggacaggtgt ccctcgcccg gcgccagtac
      721 tacgcgcagc cgcacaatgc cttctggcgc atcatgggag cgctgttcgg cgccggcctc
      781 gagcagccct acgagtcgcg cgtcgagcgt ctgaagacga aacgcgtcgc ggtctgggac
      841 gtctgcaggg ccgccacccg gcccggcagc ctcgattcgt cgatcgacgt gtcgagcgtc
      901 atcccgaacg acttcggaac gttcttccgc cgccatcccg cgatcgctgc ggtgttcacc
      961 aacggtggca cggcgacgcg gctgtaccag cgtctcgtgc agccgcgcct cgcgggcgcg
     1021 ccgcgcgagc tgccgctgca tgcgctgccg tcgacgagtc ccgcgcacgc gtcgctgcga
     1081 ttcgagcaga agctcgaacg ctggcggcag gtcgcggacg tgctggacga catcggttga
     1141 tcgcgacggc acacgcgctg cgctgggcct gcgtcgcgat cgcgagttcg gcgctcgccg
     1201 gctgcatgcc cctcacgcgg gcgccggcga gcgtgccatc gcacgaggtg ccggcagact
     1261 ccgtcgaact ctacgccgtg ggcgacatgg cggattgccg tcgcgacccg ccgggccagt
     1321 ccgccgcccg cctggtgtcg gcgctggtgc cccgcggcgc gacgctgctg gcgctcggcg
     1381 acctcgtcta tccgtacgcg gattcagaga cgctcgcccg atgctacgaa cccacctggg
     1441 gatcgcatcg cgccacgacg ctcgcggtcg cgggcaacca cgactacgtg gagaacggca
     1501 gccgcacgtt tcgcgagtac ttcgacctgg accggcgcac ggtcagcgag cgctacgtgg
     1561 cgtacaccga gaggttgtcc gcggactggc tgctggtcgt ggtcgacagc aacgtgcgcg
     1621 ggggcgcgct cgaccggcag ctcgagtggc tgcgggagac gctggagcgt gtccgcccac
     1681 gctccggggc aggcgcgtcg cctgcgtcgc cgcgttgcct cgccgtcgcc tggcacgcgc
     1741 cgctgcactc ctcggggtgg catcgcgggt cgggcgatca catgcgtccg tactgggaga
     1801 tcgtcgacgc atacggcgcc gacctggtgc tgagcggaca cgaacacttc tacgaagcat
     1861 tcgacccttt cgatgccgac ggccggcgcg tcgcgtcggg tccgcgccag ttcacggtcg
     1921 gcacgggcgg tgcgcggctc tacggcttct ggaagccgcc ttaccagagc cgcgcgcggg
     1981 tgctggaatt cggcgtgctg cacctggtgc tcgcgccggg ccgttacctg tggcgcttcg
     2041 tcgacctcga tcgtcgagtc cgtgacgccg gtacggccga ctgtcgtggc ggcgccgggg
     2101 tccctacacc ggttgatctg cggtaacgta gccggccata cgaaacgcgg tcgcaaggac
     2161 cgccaccaag acccaggacg ccatcggccc atgcaagcct tcaccggttg gatgactgcg
     2221 ctcaattcgt tcgtctgggg tccggcgatg ctcgtgctga tcctcggcac cggtttctac
     2281 ctgcagctgc ggctgcgggg catgccgctg ctgcgcatcc ccggcggcct gcggctggtc
     2341 tggggcgggc gtggcggcga ttcacagcac ggcgaggtca gtccgttcgc ggcgctgatg
     2401 acctgtctcg ccgcgacgat cggcgtcggc aacatcgcag gcgtggcgac cgccgtggcg
     2461 ctcggcggtc cgggcgccgt gttctggatg tggatgacgg cactggtcgg catggcgacc
     2521 aagtacgccg aggtgctgct cgccgtgcac taccgcgagc gcgacgcgga cggccgctgg
     2581 gtcggcggcc cgatgtatgc gatcaagaac ggcctgggcc ggcggtgggc ctggctcggc
     2641 acgctgttcg cggtgttcgg cgggatcgcc ggattcggca tcggcaacat ggtgcaggcc
     2701 aacagcatcg ctgcggcgat gcagaggtcg ttcagcgtga gcccgaccgt caccggcgtg
     2761 gtcctgaccg tgctgaccgg cgccgtggtg ctcggcggca tcaagcgcat cgctgcggtg
     2821 gccaactggc tcgtgccgtt catggccgtc ggttacgtcg tggccggcct cgtcgtgctg
     2881 gtcacgcacg cggcggcggt gccgcaggcg ttcgcgctga tcgtcgagac cgccttcacg
     2941 ggcaccgccg cggtcggcgg gttcacgggc gcggcaatga tcgccgccat ccgctacggc
     3001 gtcgcgcgcg gcatcttttc gaacgaagcc gggctcggca cggccggcat cgcgcaggcg
     3061 gcggggatga gcaacgaccc cgtgcgcagc gggctgatcg gcatgatggg caccttcttc
     3121 gacaccatcg tcgtctgctc gatcaccggc ctcgcgatcg tggtgtccgg cgcctgggac
     3181 tcgggcgaga cgggcgcgca gctcacgcag ctcgcgttcc agtcggccat gccgggcatc
     3241 ggcgacgagg tggtcgcgat ctcgctcggc gtgttcgcgc tgacgacgat cttcggctgg
     3301 tcgtactacg gcgagcgctg ctggcagttc ctggtcggca cctggacgat cagaccgtac
     3361 cgcgtgctgt ggaccgtcgc ggtgttcttc ggcgcggtca cgcacctgga cctcgcctgg
     3421 acgatcgccg acacgctcaa cgcgctgatg gccgtgccga acctgatcgc gctgctcgcg
     3481 ctgtcgcccg tcgtcgtgca gttgacgcgc gccggactcg ccgacgcgct ccggtcctga
     3541 ggtccggttc gcttataatg gctgcaccgg cgggcgctgc gcgccgggtg catgaccgtc
     3601 aagctgccac tgccgtaccg ccgatcgcgc ggcctcgcgc caggcaacga tttgctcggg
     3661 atccccggcg tcgcgctcga cgtggtcggc ctcgagatcg gcgtcgacct cgaagccggt
     3721 tggcgacggc gcgtcgagca tgcggcgctg gcgatcggtt ggccgtcccc cgcgtttgcc
     3781 cgccgtcaga cggagcaggg ccacacgctg gcctacacgg cgcctgccga ccagctgcag
     3841 acggcgcgca ccgccaacga gtgggcggtg tgtgcagcgc tggtcgaact cgacccgtgt
     3901 cactgggcat cgctgctgga gaatctgccg ggatccgtgc ctgcttccgc gcgggatgcg
     3961 gtcggccagg atgccgccat cgaccgcctc gtccgcctcg gcgctgccga ggctgcggcg
     4021 caccgagtct cctgactcac atccggctca cttctcgcgc cagacgccct cacgacgccg
     4081 gtcggccgcg ccctcgaaga cattgccgcg taccgcgatg ccgtgcaggc cggattcctc
     4141 gccgcggccg ggtttcaccg tgacgccgcg ctcggcgagc ccgctcacca cgtcgggcgc
     4201 cagcttggcc acctccccac ccacctccgt gccgcgggcg atcacgttgg gcaggtcgaa
     4261 cgccgcctgc agcggcaggt cccaggcgag caggccgagc agcgccttgg cattgtaggc
     4321 aaggatcgcc gagccgccag gcgaccccag cgcggcgacc aggtcgccgt cgtcgcgtgc
     4381 cagcacgatc accggcgcca tcgacgagcg cggccgcttg ccgccggcga cggcgttcgc
     4441 gaccttgtgg ccttccgcgt cgaccgccgt gaacgaaaaa tcggtcagct ggttgttcag
     4501 gaagaagccg ccgacggcgc ggcccgagcc gaacagcgac tcgaccgacg tggtcatcga
     4561 gacgacgtcg ccttcgtcgt cgacgaccac gaagtggctc gtgccggcca cgtcgagcgt
     4621 cgcgtcacga ccggcgcgcg tgagcgatgc acccggcggc gtgccgggct ccggcgcgtc
     4681 cgatgcggcg cgcggcccga tgcggcgcgc ccgctcgtcg agatagcgta cgtcgagcag
     4741 tccttcgacg ggcaccgcga cgtactccgg atccgcgacg tagcgatcgc gatcggcgta
     4801 catcagccgg ctggcctcgg caaacaggaa ccatgcccgc ggatcgtccg ggccgctctg
     4861 tgcgatgtcg gtgcgctcga gcaggccgag cagttcgagc aggctgacgc cgctcgacgg
     4921 cggtggcggc acgcagacca ggtagatccg gaagggtttg cagagggctg gcgtgacgag
     4981 cggccggtac gcggccatgt ccgcgagcgt cagagcgccc ggacggggcg cgtcgtgcgt
     5041 gcgcgcgacg atctgctcgg cgagcgggcc ttccagcagc gcgcgcggac cgcgcgcggc
     5101 cagcgtgcgc aacgtctgcg cataggcggg gttgcgcagc gtgtcgccgg cctggacgcg
     5161 cgtgccgtcg ggcctgctga acagcgcggt ggcatccgga agcgtcgcct gcctgaacgt
     5221 gctgttcgca aagcggccga gccgttgcgg cacgacgaac ccctgctcgg cggcgcggat
     5281 cgaggcgtcg aacagctcac gccacggtcg tttcccgaag cgctcgtggg caacgcccag
     5341 catggcgatg gcgccgggga cgccggtcgc gcggccgctg gtgacggcgt ccgcgtacga
     5401 aagcggtgcg ccgtgctcgt cgaggaacat gtcgggcgtg gcacccgcag gtgccgcttc
     5461 acggccattg aacgcggtca cggcgcgggt ctgcgcgtcg tagtgcagca tgaaggcccc
     5521 gccgcccagg ccggagctct gtggctcgac caggccgagc gcggcttgca cggcgaccgc
     5581 ggcatccacc gcgctgcctc cggcacgcag cacctccagg cccgcgtcga ccgcgatcgg
     5641 gttggccgcc gccacgccgg cgcgaaggct gaccgttgca cccgtgaccg gagccgcggg
     5701 cgccggcgca gcgtcgggcg tcggatccgt ccggcacgac gcgacgccga acgcgagcag
     5761 cagcaatgca agcgtgcggc cgggccgggc cgggcgtggc ttcgtggatg gcatgcggga
     5821 aagcgtggcg gttcggggag cccgacaata gactgtcgcc cctcccgtcg tcgagacgag
     5881 ccctcgagtc catgcgcagc gtcgtgcccg tgaccgtccg cctgagcctg gccttgtccg
     5941 tggcgctcgg ctgcagcgcg gcggtcgctc aacctgcgcc cgtcgatcga ccgccgtcgc
     6001 caggcgtggc ggtcgcgccg cgcccgacgg tcgtcgtggt gcacgcgggt cggctgctcg
     6061 atcgtcccgg ccgggcgcca cgcggcccga gcacgctggt cgtgcgcgac gggcgcgtca
     6121 gcgaagtgct cgacggtttc gtggacgtac cgggcgcgca gcgcgtgcac gaccagcgct
     6181 cgcgcttcgt cctgccgggg ttgatcgaca gccacgtgca cctcgactcc gacgcgggcg
     6241 gacaacaggc ccggctcgag gacgtccagc tcgagaccgc ccggttcgca taccaggcgc
     6301 tcgccaacgc acacaagacg ctgcgcgcgg ggttcacgac ggtgcgcaac ctcggcgacc
     6361 gctacggcgt gacgctcgcg ctgcgcgacg cgatccgcgc cggccaggtg atcggtccca
     6421 acatcgtcga cgcgggtcgc tcgatctcga cgacgtccgg gcacatggac tacgcgctcg
     6481 gctatcgcga ggacttccgc gaggtgctgc acgcgcacga caacctctgc gacggcatcg
     6541 agtcgtgccg gcgcgccgtg cgcacgcagg tcggtcgcgg cgtcgacgtg atcaagatcg
     6601 ccaccactgg cggcgtcaac agccaggttg gcgcagggct cgggcagcag atgttcgcgg
     6661 acgaggcgcg cgcgatcgtc gagacggcgc gcctctatgg caagaaggtg gccgtgcacg
     6721 cacacggcgt cgacggcatc gcggtcgcgc tcgcgtcggg cgtcgactcg atcgagcacg
     6781 gcacgatgct cgacgatgcg acgatcacga ccatggcgcg gcagggcacc tggtacgtgc
     6841 cgacgctgtc gacggtcaac ggctacatcg agcgcctggc caccaacccc gacgcgtatt
     6901 cgccggaggt gaaggccaag gtcgagtggc gcatcgaggt caccggccaa tcgctcgaaa
     6961 aggccgtgcc gcgcggcgtg aagatcgcct tcggcaccga cgccggcgtg agccggcacg
     7021 gtcgcaacgc cgacgagttc gagctgatgg tcaagcacgg catgacgccg gccgcggcga
     7081 tcgaggcggc gaccgtcaac gccgcggcgc tgctcggcct cgacgacgtc ggcacgctcg
     7141 agcccggcaa gcgggccgac ctgattgcgg tcgaaggcga tccgctggcc gacgtcacgg
     7201 tgctgaagcg cgtgccgttc gtgctcaagg acgggcgcga gttcacgggc ggggcccgct
     7261 gagcgtgggc acgcgacgcc caccggcgga agacggccgg ccgaacccct tcgtgcaacc
     7321 gctgccttac tcggacgcct gcgagcgcaa caaggagccg atcctggccg tgctgcgtcg
     7381 ctacctcgcc gacacggcgg gcctcgtgct cgagatcggc tccgggaccg gacagcacgc
     7441 cgtgcacttc gcccggcacc tgccgcaact gacgtggcag ccgaccgacc aggccgagga
     7501 actcgccacg ctgaatgccc gcgtcggctt cgaagggccc tgcaacctgc tgccgccgct
     7561 cgaactcgac gtcgccgacc gtcgatggcc gtgcgactcc gattcggtcg acgcggtgtt
     7621 cagtgccaac acgctgcaca tcatggactg gaagcacgtg cagtcgttct tccgcggcgt
     7681 cggacgcgtg ctgcggccgg aaggcctgct gatcgtctac gggcccttcc ggtactccgg
     7741 gcagtacacc tcgcacagca accacgcgtt cgacgacgcg ctgcggcgcc gcgatcccgc
     7801 gagcggcatc cgtgacttcg aggccgtcga cgcgctcgcg agcgcgcagg cgctcgcgct
     7861 cgtcgaggac gtcgcgatgc ccgccaacaa ccagacgctc atctggcgca agtccgccgc
     7921 atgaacgcgg cgtcgggcgc gtcctcctca gcgatccgct tcgacgttgg tgcggaatgc
     7981 cgtcgtgaag tcgatgcggt cgacggccgc gccgcccacc tcgacgaccg tcggttcggg
     8041 ttcgaccgga tacgcgccgc acgcctcgcc gtcgtcgcag atcgagccgt cgttgttcat
     8101 gtcggtaccg gataccacga ggtaggcgcc cggcgcgacg tcctcgatcc ggtatgacgt
     8161 cgcggtgccg agtacgcgta tcgaggtctg cgcgcccgtt gctgcgcgcg tcgtcgggtc
     8221 catcaacagg acgtactgca gtccggcgtc cggtgccagc gtgctcgcgg taacccgcag
     8281 cgcgacggtg acgcgctcgg tgcccgcgct cgacacgaaa tcgacccagc caccgtggct
     8341 gcccgacggc agcgtcgagc gctccgcgtc gatcgcgtag cgcccgagac cgtggtcgtc
     8401 gaccgccgcg ggcgtgacgc ggagccacgg atcggacgtg cggacttcgg tgatcgacac
     8461 gtcgcccgag ccgccgttgg cgacgctgac gtccgcgtgc gtgccgacgt cgccgaggtt
     8521 gatcg
//
LOCUS       DASPFR010000234         8823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1288752, whole genome shotgun sequence.
ACCESSION   DASPFR010000234 DASPFR010000000
VERSION     DASPFR010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8823
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288752"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1828)
                     /locus_tag="VD737_06060"
     CDS             complement(167..1828)
                     /locus_tag="VD737_06060"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HXW10157.1"
                     /translation="MTEPSRWARIACALLLTSLPATAAVGAASDLPHDWPRGLPGGEE
                     LEAAGAVIGEIEILTEDVFDPERPDESGWVYRTANKLHIVTRQKVIRSQLLFKPGQPY
                     VHRVVQETERILRANDYLYDAVIRPTSFDGTTVNLQVRTRDTWTLNPGINFNRQGGEN
                     SGSLELEEKNLLGTGQQLAFGWTNDVERESIDFDYFDPHFRSTWTRLGVRYSDADDGE
                     TMALRFERPFYALDTARAGGLFLFDTQFEEPRYAYGDRVGRIQHGQEFYEAWGGWSDG
                     WENGWVRRWSAGATYDRNRFTEVPGEPLGGPLPEDRMLAYPWIGFDIIQDAYEERSNQ
                     DQIERTEDVLVGFRAGGRLGYAAEAFGSDRDAWLMSLYAQDGWDLGNESSVFVGLTGS
                     GRLEDDGLRDGVLSAEARYYARTSKRTKFFATVSGTVSEELDAEDQLVLGGDTGLRGY
                     PLRYQAGTSLALLTLEERYYTNWYPFRLFHVAGAVFFDMGRTWGTDVTGATSDGLLKD
                     VGLGLRLGSSRSAFGNVIHIDLAFPLDGGDDIDDVQFVVETKSRF"
     gene            complement(1828..2799)
                     /locus_tag="VD737_06065"
     CDS             complement(1828..2799)
                     /locus_tag="VD737_06065"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HXW10158.1"
                     /translation="MPQAIRVHQYGGPEVMLWEDLETRPPDKGEALVRHTAIGLNFID
                     VYERTGLYGGVLPTGLGHEAAGVVEAVGRGVRGIAVGQRVAYAVSTPGAYAEEAVVRA
                     DRLVPVPDDVSDRLAAAALLKGLTAQVLLRRTYRVARDTVVVVHAAAGGVGSILVQWA
                     AHLGARVIAVVGSAAKADAVRALGCRDVLVQGEDLAVRVKELSDGQGAHVVYDSVGKD
                     TFFASLDSLRPLGLMVSYGNASGPVPPIAPLELARRGSLFLTRPVLFSYVTRRSGLLR
                     SAKELFDVVGHGVVRIEIGQTYALRDVQRAHRDLQERRTVGSTVLLP"
     gene            complement(2932..3309)
                     /locus_tag="VD737_06070"
     CDS             complement(2932..3309)
                     /locus_tag="VD737_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10159.1"
                     /translation="MPDPAVTEALESAVAKLHIGRQRRHVFLCVAGGKCATEARSLES
                     WAYLKRRIKELGLHDVDGGVMRTKVHCLRVCVGGPIAVVYPDGTWYRKCTPENLERIL
                     QSHLIGDRPVRDLVIADAPLPPP"
     gene            complement(3366..3704)
                     /locus_tag="VD737_06075"
     CDS             complement(3366..3704)
                     /locus_tag="VD737_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXW10160.1"
                     /translation="MKLISAIIKPFKLDDVRAALSELGVSGMTVTEVKGFGRQRGHTE
                     LYRGAEYVVDFVPKTRIEVAVKESLVDQVIEAIVGAARTGKVGDGKIFVTDLERAIRI
                     RTGEADDQAL"
     gene            4049..5266
                     /locus_tag="VD737_06080"
     CDS             4049..5266
                     /locus_tag="VD737_06080"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXW10161.1"
                     /translation="MTDNTVVIVGASHAAVQAIDTLRREGHAGRIVLVGDEAFLPYNR
                     PPLSKKYLSGELERERLLLRSPTFYENSHVEVRLGVRATAIDRADQRVRLSDGDELRY
                     ERLLLCIGSRNRVLEVPGRDLRGIHYLRTMADVDGIRTELAAARRLVVVGAGYIGLEA
                     AASARHLGLDVTVLEMADRPMNRVVAPEISEFYRSRHEREGVRVLCRTSVTSFTGSPD
                     GRVRAVVCGDREFDCDFVIVGVGIVPETALPASAGLKCDNGISVDEHCQTSDPHIYAA
                     GDCTCHPSVRYGRRLRLESVDNAVEQARTAAMNICGKPARHAHVPWFWSDQYDLKLQI
                     AGLSTGYDQTVVRGDPDTGRFALYYLAEGELLAVDAVNSPKDFMTGKKWIAERKRPDP
                     AKLADTEVDLKTL"
     gene            5300..7069
                     /locus_tag="VD737_06085"
     CDS             5300..7069
                     /locus_tag="VD737_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXW10162.1"
                     /translation="MNDTAGRPQALGRTSISDDASRELLAAIGQWTGGMSPQAFGGAW
                     LNVVSRLALAPGRQLELAGQAFRKSIELAQFAGQAVRGVDEPVAPQVAGTPYASRFSD
                     EAWARFPFNVLAQAFVTGAELAREAVRQVPGAEPDAQYVAGFTVREGLELLAPDNYLP
                     TNPQLIAQTREERGQNLLRGLRNLADDVQRTFKGQGPVGVENYVVGKHTAVTPGKVIL
                     RNSLMELIQYAPATEDVHAEPVLIVPAWIMKYYILDLSPGNSLVKYLTDMGHTVFMVS
                     WKNPTAEDRDVSMDDYVSLGVLAALDAIGQVVPRRKVHAVGYCIGGTLLAISAATLAQ
                     RADERLASATLFAAQTDFSEPGELSVFISPAQLAMLEALMWKKGVLESRQMGGAFQLL
                     RTYDLLWAPSVQSYLKGERLGVNDLMAWNADGTRMAYRMHTDYLHQLYLDNDLAQGRY
                     VALGETIDLASIEVPMFVVGTETDHVAPWKSVYKVGKLVRSKDYTFCLTSGGHNAGII
                     SGPQHPRRRHRVLTMKTGARLPSAEQYLQKVEPQQGSWWPTFAAWLAAHSSPRRVAPP
                     KMGNAKKGLKPIGDAPGEYVLAR"
     gene            complement(7093..8184)
                     /locus_tag="VD737_06090"
     CDS             complement(7093..8184)
                     /locus_tag="VD737_06090"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXW10163.1"
                     /translation="MLQRLFAIMNKEVRQLARDRLTFGMIVGIPLMQILLFGYAINFD
                     VRDIRAAVVDEANTSHSRALVADLQASGVVQFGAPSASVAELRRRLDAGEISVGVVIP
                     RDFERRRLGNERAVVQLLVDGSEPMIDSVARGLAQVPLPGRDGEYRSRVPVFEVRTEY
                     NPERRTAVQVVPALVGVILNMTMVIFTSVAIVRERERGNLELLITTPIRSWELMAGKL
                     MPYVLIGLVQTTIVLAVGSWVFDVPMNGSLLDLYLAAALFIAATLTLGLVISTLVKSQ
                     MQAFQLSFLTLLPSILLSGFVFPFDGMPEPAQWIAQVLPLTHFVEMIRGILLRGAPLG
                     ELGLPAAKLGVFLAVTLAIATSRFHKRLD"
     gene            complement(8200..>8823)
                     /locus_tag="VD737_06095"
     CDS             complement(8200..>8823)
                     /locus_tag="VD737_06095"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXW10164.1"
                     /translation="GKVRTARIEERLSQYSLQDRREQRAGTLSGGQKQRLALAAATLH
                     EPELLLLDEPTSAVDPQSRRDFWESLFALVARGVTILVSTHYMDEAERCHELAILADG
                     RLVAQGAPKQLMGDIPARVVEVETDDTVSARAVLVQDEAVLSVAQLGTRLHVLLRPDT
                     PEAAQRCAARLRDAGVAAQTTAVGASLEDVFVAATGFKRTSHVAVAG"
BASE COUNT         1383 a         3077 c         3006 g         1357 t
ORIGIN      
        1 cagcggttag cggttagcgg tttagcggtt agcggtttag cggttagcgg ttagtgaata
       61 gcggttagtc ggaagctcct gcggcgctgc gacgcccact tcttactaac cgctaaccgc
      121 taaccgctaa ccgctaaccg ctaaccgcta accgctggac acttcctcag aaccgggact
      181 tcgtctccac cacgaactgc acgtcgtcga tgtcgtcgcc gccgtcgagc gggaatgcca
      241 ggtcgatgtg gatcacgttg ccgaacgccg agcggctcga gccgagacgc aggccgaggc
      301 ccacgtcctt gagcagcccg tcgctggtcg caccggtgac gtcggtgccc caggtgcggc
      361 ccatgtcgaa gaacaccgcc ccggcgacgt ggaacagccg gaatggatac cagttcgtgt
      421 agtagcgttc ctcgagcgtc agcaaggcga gcgacgtgcc cgcctggtag cgcaacgggt
      481 agccgcgcag cccggtgtcg ccgccgagca ccagttggtc ttcggcgtcg agctcttcgc
      541 tgacggtgcc gctcaccgtg gcgaagaact tggtgcgctt ggaggtcctg gcgtagtaac
      601 gcgcctccgc cgacagcacg ccgtcgcgaa ggccgtcgtc ctcgagccgc ccggatcccg
      661 tcagccccac gaacaccgag ctctcgttgc cgagatccca gccgtcctgg gcgtagaggc
      721 tcatcagcca cgcgtcacgg tctgacccga aggcctccgc cgcgtaaccg agtcggccgc
      781 cggcgcggaa gccgaccagc acgtcctcgg tccgctcgat ctggtcctgg ttgctgcgct
      841 cctcgtacgc gtcctggatg atgtcgaagc cgatccatgg ataggccagc atgcgatcct
      901 cgggcagcgg cccgcccagg ggctcgcccg gcacctcggt gaaacggttg cggtcgtacg
      961 tggcgcccgc gctccagcgc cgcacccagc cgttttccca tccgtccgac cagccacccc
     1021 aggcctcgta gaactcctgg ccgtgctgga ttcggccgac ccggtcgccg taggcgtagc
     1081 gcggctcctc gaactgcgtg tcgaagagga acaggccgcc ggcacgcgcg gtgtccagcg
     1141 cgtagaacgg acgctcgaac cgcagcgcca tggtttcgcc gtcgtccgcg tcgctgtagc
     1201 ggacgccgag ccgcgtccac gtcgaacgga agtgtggatc gaagtagtcg aagtcgatcg
     1261 actcacgctc cacgtcgttg gtccagccga acgcgagctg ctggccggtc cccagcaggt
     1321 tcttctcctc gagctcgagg gaaccggaat tctcgccgcc ctggcggttg aagttgatgc
     1381 cgggattcag cgtccaggtg tcgcgcgtgc gcacctggag gttgaccgtg gtgccgtcga
     1441 acgacgtcgg ccggatcacg gcgtcgtaga ggtagtcgtt ggcgcgcagg atgcgctcgg
     1501 tttcctgcac gacgcggtgc acgtacggct ggccgggctt gaacagcagc tggctgcgga
     1561 tcaccttctg ccgcgtgacg atgtgcagct tgttggcggt gcgatagacc cagccgctct
     1621 cgtcggggcg ctcgggatcg aacacgtcct cggtgaggat ctcgatctcg ccgatcacgg
     1681 caccggccgc ctcgagttct tcgccgcccg gcaggcctcg tggccagtcg tgcggcaggt
     1741 cactggcggc gccgacggcc gccgtcgccg gcagcgacgt cagcagcagt gcgcaggcga
     1801 tccttgccca gcgcgatggt tccgtcatca aggcagcagc acggtcgaac cgaccgtgcg
     1861 tcgctcctgc aggtcccgat gggcccgctg tacgtcacgc aacgcatagg tctggccgat
     1921 ctcgatgcgg accacgccgt gaccgacgac gtcgaagagc tccttggccg atcgcagcag
     1981 gccggagcgg cgcgtgacgt agctgaacag gacggggcgc gtcaggaaca gcgagcctcg
     2041 acgcgcaagc tcgaggggcg cgatcggggg gaccggaccc gacgcattgc cataactcac
     2101 catgagtccc agtggccgca ggctgtcgag cgacgcaaag aacgtgtcct tgccgaccga
     2161 gtcgtagacg acgtgcgcgc cctgcccgtc gctgagctcc ttgacgcgca ccgccaggtc
     2221 ctcaccctgg acgagcacgt cccggcagcc gagcgcgcgc accgcatcgg ccttggcggc
     2281 gctgccgacc accgcaatca cgcgggcacc gaggtgcgcc gcccactgca cgaggatgct
     2341 cccgacgcca cccgccgccg cgtgcacgac gaccacggtg tccctggcca cccggtaggt
     2401 gcgccgcaac agcacctggg ccgtcaggcc cttgagcagc gccgcggccg ccagccggtc
     2461 gctcacgtcg tccggtacgg gcacgagccg gtccgcccgc accacggctt cctcggcgta
     2521 ggcgcccggc gtcgacacgg cataggcaac gcgctgcccc acggcgatgc cgcgcacgcc
     2581 gcgtccgacc gcttcgacga ctccggcagc ctcgtgccca aggcctgtgg gcagcacgcc
     2641 gccatacagg ccggtgcgtt cgtagacgtc aatgaagttg aggccgatcg ccgtgtgccg
     2701 gacgagcgct tcacctttgt cgggcgggcg ggtctcgagg tcctcccaga gcatcacttc
     2761 gggcccgccg tactggtgaa cacgtatcgc ctgtggcatg ggactcgccc cgctcgtcgt
     2821 ccggtggtgt gccggatcat gacgaggggc ccgccattcc gatagtggac cacggctcga
     2881 acgtgccgtt ccgccgtgtg agacggcacc ggcagcgaaa atcagtcgcg cctacggcgg
     2941 cgggagcgga gcgtccgcga tcacgagatc gcggaccggc cggtctccga tcaggtgcga
     3001 ctgcaggatg cgttcgaggt tctcgggcgt gcacttgcga taccaggtgc cgtccggata
     3061 gacgaccgcg atcggtccac ccacgcaaac ccgcaggcag tgcaccttgg tgcgcatgac
     3121 gccgccgtcg acgtcgtgca ggcccagttc cttgatgcgg cgcttcaggt acgcccagct
     3181 ctcgagcgag cgcgcctcgg ttgcgcactt gcccccggcc acgcagagga acacgtggcg
     3241 acgctggcgt ccgatgtgca gcttggcgac ggcgctctcg agcgcctcag tgacggcggg
     3301 gtcgggcatg aagggctcgg ttccgggcgg ccggaaccga tgctaccccg gcgaggatgc
     3361 gcgcgtcaga gcgcctggtc gtccgcctcg ccggtgcgga tccggatggc gcgctcgagg
     3421 tcggtcacga agatcttgcc gtcgcccacc ttgccggtgc gcgctgcgcc cacgatggcc
     3481 tcgatcacct ggtcgaccag cgattccttt accgcgacct cgatgcgcgt cttcggcacg
     3541 aaatcgacga cgtactccgc gccgcggtac agctcggtgt gtccgcgctg gcgcccgaaa
     3601 cccttcacct ccgtcacggt catgccggag acccccagct cggacagggc ggcgcggacg
     3661 tcgtccagct tgaagggctt gatgattgcg ctgatcagct tcattagctt aaggctcctc
     3721 gttctttcga tcattgtccg gcggcgggac gcggctccac ttgcgtggaa accgaaacgg
     3781 ccgtcgtcgc agcgcctccc ccacgacggg ggccttgcgc cgtcgatgca gtttccgtgc
     3841 cgacccagcc ggaccacggt ttcgtgcgtc gcggcgacga gcgacgcacc accgtggcgc
     3901 gcctccttcc cgtcctgccc acggctggtg cgcgtcgcgg cgccacagga tagcgacggc
     3961 gcgctccgga cgcacccgtc gagggacgtc gcgccgcttt gttgctttgc ggcatcatag
     4021 tcgccatctc ggcagcaacg gatccggaat gacggacaac acggtggtga tcgtcggcgc
     4081 gagccacgcc gcggtgcagg ccatcgacac gctgcgacgc gagggtcacg ccggccgcat
     4141 cgtgctcgtc ggtgacgagg cgttcctgcc ctacaaccgg ccgccgctgt cgaagaagta
     4201 cctctcgggc gaactcgagc gggagcggct gctgctgcgc agcccgacgt tctacgagaa
     4261 ctcgcacgtg gaagtgcggc tcggcgtgcg cgcgacggcg atcgaccgcg ccgaccagcg
     4321 cgtgcgcttg agcgacggcg acgagctgcg ctacgagcgg ctgctgctgt gcatcggcag
     4381 ccgcaatcgc gtgctcgagg tgccggggcg cgacctccgt ggcatccatt acctgcgcac
     4441 gatggccgac gtcgacggca tccggacgga acttgccgcg gctcgacgcc tggtcgtcgt
     4501 cggagccggc tacatcggtc tcgaggccgc cgcgtccgcg aggcacctcg ggctcgacgt
     4561 gaccgtgctc gagatggccg atcgcccgat gaatcgcgtg gtcgcgccgg agatctccga
     4621 gttctaccgg agccggcacg aacgcgaagg cgtacgggtg ctctgccgca cgtcggtgac
     4681 gtcgttcacg ggctcgccgg acggccgggt gcgtgccgtc gtgtgcggcg accgcgaatt
     4741 cgactgcgac ttcgtgatcg tcggggtcgg tatcgtgccg gagacggcac tgccggcctc
     4801 ggccggcttg aagtgcgaca acggcatctc cgtcgacgag cattgccaga cctccgatcc
     4861 gcacatctac gcggccggcg actgcacctg tcacccgagc gtgcgctatg gccggcggct
     4921 gcggctcgag tccgtcgaca atgccgtcga gcaggccagg accgcggcca tgaacatctg
     4981 cggcaagccc gcgcgccacg cgcacgtgcc gtggttctgg tccgaccagt acgacctcaa
     5041 gctgcagatt gccggcctct ccacgggcta cgaccagacg gtcgtgcgcg gtgatccgga
     5101 taccggccgg tttgcgcttt actatctcgc cgagggcgag ttgctggccg tcgacgccgt
     5161 caacagtccg aaggacttca tgaccgggaa gaagtggatc gccgagcgca agcgacccga
     5221 tccggcgaaa ctcgccgaca ccgaggtcga cttgaagacg ctctgacgtc ccggtctgcc
     5281 gccgcaggag aactctgtca tgaacgacac cgccggacgc ccgcaggcgc tgggccgcac
     5341 ttcgatttcc gacgacgctt cccgcgaact gctcgcggcg atcggccagt ggaccggcgg
     5401 catgtcgccg caggcgttcg gtggcgcctg gctcaacgtc gtgtcgcggc tcgcgctggc
     5461 gccaggacga caactcgagc tcgccggcca ggcgtttcgc aagtcgatcg agctcgcgca
     5521 gttcgccggg caggccgtgc ggggcgtgga cgagccggtc gcgccgcagg tggcgggcac
     5581 cccctacgca agccggttca gtgacgaggc gtgggcccgg tttccgttca acgtgctggc
     5641 gcaggccttc gtgaccggcg ccgagctcgc tcgcgaggcg gtgcgccagg tgccgggcgc
     5701 ggagcccgac gcgcagtacg tggcggggtt caccgtgcgc gaggggctcg aactgctcgc
     5761 gccggacaat tacctgccca ccaatcccca gctgatcgcg cagacccgcg aggagcgtgg
     5821 acagaacctg ctgcgcggcc tccgcaacct cgcggacgac gtgcagcgca cgttcaaggg
     5881 acaggggccc gtcggtgtcg agaactacgt cgtgggcaag cacacggccg tcacgcccgg
     5941 caaggtcatc ctgcgcaatt cgctgatgga actgatccag tacgcgcccg cgaccgagga
     6001 cgtccatgcc gagccggtgc tgatcgtgcc ggcgtggatc atgaagtact acatcctcga
     6061 cctgtcgccg ggcaattcgc tcgtgaagta cctgaccgac atgggtcaca cggtcttcat
     6121 ggtctcctgg aagaacccga cggccgagga tcgcgacgtc agcatggacg actacgtgtc
     6181 gctcggcgtg ctcgcggcgc tcgacgcgat cggccaggtg gtgcccaggc gcaaggtgca
     6241 cgcggtcggc tactgcatcg gcggcaccct gctggcgatc agcgcggcca cgctcgcgca
     6301 gcgcgccgac gaacggctcg cgagcgcgac gttgttcgcg gcgcagaccg acttcagcga
     6361 acccggcgag ctctcggtgt tcatcagccc ggcgcagctc gcgatgctcg aggcgctgat
     6421 gtggaagaag ggcgtgctcg agagccggca gatgggcggc gcgttccagc tgctgcgcac
     6481 gtacgacctg ctgtgggcgc cctcggtgca gtcgtacctg aagggcgagc ggctcggcgt
     6541 caacgacctg atggcctgga acgccgacgg cacgcgcatg gcctatcgca tgcacaccga
     6601 ctacctgcac cagctgtacc tcgacaacga cctggcgcag ggccgctacg tcgcgctcgg
     6661 cgagaccatc gacctggcct ccatcgaggt gccgatgttc gtcgtcggca cggagaccga
     6721 ccacgtggcg ccgtggaagt ccgtgtacaa ggtcgggaag cttgtgcgct cgaaggacta
     6781 cacgttctgc ctcacgagcg gcggccacaa cgccggcatc atcagcggac cgcagcatcc
     6841 gcggcgccgg catcgcgtgc tgaccatgaa gacgggcgcg cgattgccgt cggccgagca
     6901 gtacctgcag aaggtcgagc cgcagcaggg ctcctggtgg ccgacgttcg ccgcctggct
     6961 ggccgcgcac tcgagccctc gacgcgtcgc gccgccgaag atgggcaatg cgaagaaggg
     7021 gctcaagccc atcggcgacg cgccgggcga gtacgttctg gcgaggtgac cagcgcgggc
     7081 ggcctcgcgc cgtcagtcca ggcgcttgtg gaaccgcgag gtggcgatcg cgagggtgac
     7141 cgcgaggaac acgcccagct ttgcggccgg cagccccagc tcgccgagtg gtgcgccccg
     7201 cagcaggatg ccgcggatca tctcgacgaa gtgcgtcagc ggcagcacct gcgcgatcca
     7261 ctgcgccggc tcgggcatgc cgtcgaacgg aaacacgaag cccgacagca ggatcgacgg
     7321 cagcagcgtc aggaacgaca gctggaaggc ctgcatctgc gacttcacca gcgtcgagat
     7381 cacgaggccg agcgtcagcg tcgccgcgat gaacagcgcc gcggcgaggt acaggtcgag
     7441 caggctgccg ttcatcggca cgtcgaacac ccacgagccc accgccagca cgatcgtggt
     7501 ctggaccagc ccgatcagca cgtagggcat cagcttgccg gccatcagct cccacgagcg
     7561 gatcggggtg gtgatcagca gttcgaggtt gccgcgctcg cgctcgcgca cgatcgcgac
     7621 cgaggtgaag atcaccatag tcatgttcag gatcacgccg accagcgccg gcacgacctg
     7681 cacggccgtg cgtcgttccg ggttgtactc ggtgcgcacc tcgaacaccg gcacgcgcga
     7741 gcggtactcg ccgtcacgac ccggcagcgg cacctgcgcg agcccgcgcg cgacgctgtc
     7801 gatcatgggc tcggagccgt ccacgagcag ctgcacgacc gcgcgctcgt tgccgagccg
     7861 gcgccgctcg aaatcgcgcg ggatcacgac gccgacgctg atctcgccgg cgtcgaggcg
     7921 gcgtcgcagc tccgccacgc tggcgctcgg cgcgccaaac tgcactacgc cgctcgcctg
     7981 caggtcggcg acgagcgccc gggagtgact cgtgttggcc tcgtcgacga ccgccgcgcg
     8041 gatgtcgcgg acgtcgaaat tgatcgcgta gccgaacagc aggatctgca tcagcggaat
     8101 gccgacgatc atgccgaacg tcaggcggtc gcgcgcgagc tgccgcactt ccttgttcat
     8161 gatggcgaac aggcgctgca gcatgcgcgg cgtccctggc tagccggcca cggcgacgtg
     8221 ggacgtgcgc ttgaagccgg tcgccgccac gaacacgtcc tcgaggctcg cgccgaccgc
     8281 ggtcgtctgc gcggccacgc ccgcgtcccg cagccgcgcg gcacaacgct gcgcggcctc
     8341 gggcgtgtcg gggcgcagca gcacgtgcag gcgcgtcccg agctgggcca cgctcagcac
     8401 cgcctcgtcc tgcaccagca cggcccgtgc cgacaccgtg tcgtccgtct ccacctcgac
     8461 cacccgcgcc gggatgtcgc ccatgagctg cttcggcgcg ccctgcgcga cgagccggcc
     8521 gtcggcgagg atcgcgagtt cgtggcagcg ctcggcctcg tccatgtaat gcgtcgacac
     8581 caggatcgtc acgccgcggg ccacgagcgc gaacaggctt tcccagaagt cgcgccggct
     8641 ttgcgggtcg acggcgctgg tcggctcgtc gagcagcaac agctcgggct cgtgcagggt
     8701 tgcggcagcg agcgcgagcc gctgcttctg tccgccgctc agggttccgg cgcgttgctc
     8761 gcgccggtcc tgcaaggagt actgcgagag tcgttcctcg atgcgcgcag tgcgcacctt
     8821 gcc
//
LOCUS       DASPFR010000235        11301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_800496, whole genome shotgun sequence.
ACCESSION   DASPFR010000235 DASPFR010000000
VERSION     DASPFR010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11301
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800496"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="VD737_06100"
     CDS             complement(<1..1225)
                     /locus_tag="VD737_06100"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXW10165.1"
                     /translation="MIPPRDILDYMVAELRVGARAPEDVLAHVRDAWVWDEATAGQLQ
                     ADIHEAHDGGRLTDAEAGDLSRGIDAWLLSMADEDPTEVIDDPAIVAMHLTRVSRDEP
                     SLELRPGVILGDRYLLGEPVARGGQSIVFRARVLRDDGDGAASGKAVAIKVLKDANPS
                     VAAIARLKREFRQAQSLQHVGVASMFDIGCDRGTWFIAMELLEGMSLRTRLNRVATQP
                     LGKPEALQIVAACADVLARAHRQGFAHGDVKPGNLFLQEDGAIKVLDFGAAYDVQPAA
                     PSTPNEVRGPSAATRTYASPEVLGGLPAEPRDDIFSLACVAYEMLAGRHPYSRMPANE
                     ARAHALEVVPVAGLTPGQNAALRNALEWSRSRRPSSIEAWLEAVLDENPGVAARKSRP
                     TTEPLTPAEWLPWRWI"
     gene            complement(1313..1777)
                     /locus_tag="VD737_06105"
     CDS             complement(1313..1777)
                     /locus_tag="VD737_06105"
                     /inference="COORDINATES: protein motif:HMM:NF001095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HXW10166.1"
                     /translation="MQLDPHVYVDADACPVKAEVAKVAERHGLAVTYVSNAWLQAPRG
                     PRLRLQVVSGSFDAADDWIVERVVQADVVVTADIPLAARCLKKGARVLGPSGRPFTGD
                     NIGNALATREIMADLRAYGTGGGPAPFGPRDRSRFLEALEHVVRDAKRGPAG"
     gene            1871..2578
                     /locus_tag="VD737_06110"
     CDS             1871..2578
                     /locus_tag="VD737_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXW10167.1"
                     /translation="MFQAIGSRSVPAILPIALAIMFAASPATAIAQVTVLLPTEGDLQ
                     ATLALPGDIRRGQEAYAECQTCHRKDASGRAKFNMPRLSGQHASVLIKQLMDIRSGLR
                     VNPDMREYMLDSDLTLQDFADMAAYLQSLPVTGNIAKGPPELVPRGQALYASDCSGCH
                     GEHGEGRGELFFPMLASQHYGYLLRELDLILTGERGNSNPAMPPLLKDYSADDKRALA
                     AYLAQLPAPARGTAPAD"
     gene            2654..3709
                     /locus_tag="VD737_06115"
     CDS             2654..3709
                     /locus_tag="VD737_06115"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXW10168.1"
                     /translation="MNDLRRVWRVSRAGSLDRLRLQEESLPPPGPEEARVRVEAIGLN
                     FADIFACQGLYSATPSGSFVPGLECAGVVESLGTEARRLSDLRPGDRVIALTRFGAYA
                     TALNVGVQYLRRVPEGWTMEQAAAWAVQGLTAWHGLVVLGAVQRNDVVLVQSAAGGVG
                     LLALDVAEALGARPIAVVGHDDKGEFLVRQRGIAPSSVIVRDRKSFGPRVDAATRALG
                     VDGLDCVLDAVLGPTFRPAFERLRPEGRYVLYGAADFMSQASRPDYLVLGWRYLRRPR
                     LDPLAMISANRSVLAFNLIWLWEQAERLPEAYAALQRLLPRPPHVGARLPFDRAPEAM
                     RLLQSGKTIGKVVLMVA"
     gene            complement(3738..4241)
                     /locus_tag="VD737_06120"
     CDS             complement(3738..4241)
                     /locus_tag="VD737_06120"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HXW10169.1"
                     /translation="MRPTAPAVPERGVMRMFDWVQQDFMPQLAAAVQVIVAAALGAVV
                     GIEREMANRPAGLRTHAITAGTAALLVVLTDYLVFEMLADTGNHAVQADPIRMVQAIV
                     IGVSFLGAGTIFRSRENGIVGLTTGASLLLVAGIGIAVGVGLLYLAIMVTAFGLLVLR
                     GLARFER"
     gene            complement(4364..5434)
                     /locus_tag="VD737_06125"
     CDS             complement(4364..5434)
                     /locus_tag="VD737_06125"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HXW10170.1"
                     /translation="MFRKCVQTLLLAMISLAVHVRAQPVEAETYTRTVHFEVKAPEAS
                     AAFLCGNMTGWKCDQIPMRRTGAGVFYVNAPLRPGRVEYVFMVDGQPRLDDASMPEND
                     AGVGQRSWFVLDDDRDAQPLRGIPQGRIEQRTFESKALGEPRAINVYTPPGYTRKHAL
                     PLLILLHGYGMNADQWLTGGVQHYLDRYIAAGALKPMVVVMPGAPDEFYTGRSEEFVV
                     KELYPWIAREYAIRQDAGAAAIGGMSMGGFGALYLAYRHPELFGHAMVLSPGNLDHDF
                     LDRFDGELGRGARVAASLDIRVGRSDDLSFPYAERLTAVLSAHGVPFSYEVSRGIHDW
                     NYWHSVMKPSLLRVQAFFDKPR"
     gene            complement(5594..8065)
                     /locus_tag="VD737_06130"
     CDS             complement(5594..8065)
                     /locus_tag="VD737_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXW10171.1"
                     /translation="MIAVLGGLTGSHVATAQQTPATTTPRDLEQIIVTGTRVENRSAL
                     ETAVPVDVVSSAALENVGVVELNQALSIALPSYNFPRPGLADGTDTIRPATLRGLSPD
                     QTLVLLNSKRRHAASLVNVNGTIGRGASAVDLNTIPNAAVQTIEVLRDGASAQYGSDA
                     IAGVINVRLRENDDGGDVTLSYGQRESSYDTPTGPLPAGATWSAPSKIERDVSDGETF
                     TVSAWKGLGLGDSGFLTLAGEYKEQDRTERGGYDFRQQYPLVGTAFDPREQDFDRFNA
                     WYGEPELDQLTFFANAGSDLANGAHLYGWASYQNREARSAGFFRRALDDRNVIEIYPD
                     GFLPIIAPEVDDYSAAAGIAWALGEWDMDTSLVYGRNEMDFTIENTLNRSLGPASGTK
                     FDAGGFAYDQLVLNFSGVRQVELGALASPLNVAAGIEARQERYEINAGEPDSYRNGGV
                     LLPNGNPTQSGAQVFPGFRPSNEVDEDRTAVGAYVDLEANLTEKFLGSAAVRFEDYSD
                     FGNNVSGKLAGRYDFTESFALRGSVQNGFRAPSLQQQYFATTSTNFINGVPFDITTFP
                     VSDPVAQALGAQPLDAEESINLSLGAVFRLAAVSITIDAYRIDIEDRIVLSENLTQAN
                     VREYLQGLGFIGVGGGRFFINGVDTETTGVDVVVNWPWETGTAGTFDFTLAGNYTTTD
                     VTKVPQTRELEALDPPPPLFDRINVLTLEEGTPQEKLAAIVNWTGDRLGATLRATYYG
                     EALDPGTTDALDFTLGAKTLVDLEGRFDVTENIRVSIGAENVLDEYPDPFEPNRNTTG
                     NTSFSNYAPFGRSGRFIYGRVSLDF"
     gene            8557..10479
                     /gene="glgB"
                     /locus_tag="VD737_06135"
     CDS             8557..10479
                     /gene="glgB"
                     /locus_tag="VD737_06135"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HXW10172.1"
                     /translation="MPAGFSPTPTTIDRCRLTDHDIYLFKQGTHTRLHEKMGAHSVSD
                     AGRAGVHFAVWAPNARRVSVIGDFNDWRANHDPLAPRSDGSGIWEGFVAGVGHGATYK
                     YRIESQGRALEKGDPFARCWEVPPRTASRVWQGEYEWRDQQWMQQRGTRNAADAPMSI
                     YEVHLGSWRRHPENGARSLTYREMAEQLPAYVSALGFTHVEFMPLMEHPFYGSWGYQT
                     TGYFAATSRYGTPEDLKYLVDALHGLGIGVIFDWVPSHFPGDAHGLACFDGTHLFEHQ
                     DRRQGFHPEWQSWIFNYGRHEVRAFLASSAMYWLDEFHADGLRVDAVASMLYLDYGRK
                     DGEWIPNAHGGRENLDAVEFLRQLNEAACRDQPGTKTIAEESTAWPGVTRPTSDGGLG
                     FGMKWNMGWMHDTLEYFKRDPVHRRFHHDELKFSLWYAFSEDFVLPLSHDEVVYGKGS
                     LLGRMPGDEWQRFANLRLLYGYMWTHPGKKLLYMGGEFGQWSEWNHESSLDWQLLERP
                     LHRSLSQWIGDLNRVLRQRGALHTLDFDERGFRWVDASDAGQSVLAYLRRGAGDEYVL
                     VACNFTPVPRHNHRLGVPVEGRWREILNSDAAPYGGSGQGNLGGATTVPVAAHGFLQS
                     IVVTLPPLAVVMFEPE"
     gene            10545..>11301
                     /locus_tag="VD737_06140"
     CDS             10545..>11301
                     /locus_tag="VD737_06140"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HXW10173.1"
                     /translation="MTKRGQKDTVTSRAAPAARRSDSTPVASVATTHRATAHDVRVRA
                     IIEDVRPRVDGGRFPIKRAVGDEVFVEADVFTDGHDVPRALLRHRRSGESDWIEVEMT
                     HLVNDRWRGRFVVDALGRYEYTVVAWTDRFATWRREFERRDEASDILIALRAGVGLLE
                     SAAARAGGEDAERLRAVALSWTAVEDPDEARRAGLADDVHELVGRHPDRRFETVHEPV
                     LCVVVDPVLARCGAWYEFFPRSASDDPGRHGTFR"
BASE COUNT         1777 a         3762 c         3902 g         1860 t
ORIGIN      
        1 cgatccagcg ccagggcagc cactcggccg gtgtcaaagg ttcggtcgtc ggccgcgact
       61 tcctggccgc aacaccaggg ttctcgtcca gcaccgcctc gagccatgcc tcgatcgacg
      121 atgggcgtcg gctgcgcgac cactcgagcg cgttgcgcaa cgcggcgttc tgtcctggcg
      181 tgagccccgc caccggcacg acttcgagtg cgtgggcgcg ggcttcgttc gcaggcatgc
      241 ggctgtaggg atgccggcca gcgagcatct cgtacgccac gcaggccagg ctgaagatgt
      301 cgtcccgcgg ttcagccggg agcccgccga gcacctcagg gcttgcgtag gttcgggtgg
      361 cggccgaagg ccctcgaacc tcgttggggg tcgagggagc cgccggctgc acgtcatagg
      421 cggcgccgaa atcgagcacc ttgatcgcgc cgtcctcctg caggaagagg ttgccgggct
      481 tcacgtcacc gtgggcgaat ccctgccggt gcgcgcgcgc cagcacgtcc gcgcacgcgg
      541 caacgatctg cagggcctcg ggtttgccaa gcggctgcgt tgccacgcgg ttcagccggg
      601 tgcgcaagga catgccctcg agcagttcca tggcgatgaa ccaggtgccg cgatcgcacc
      661 caatgtcgaa catgctcgcg acgccgacgt gctgcagcga ctgcgcctgc cggaactcgc
      721 gcttgagacg cgcgatggca gcgaccgacg ggttggcgtc cttcaggacc ttgatcgcaa
      781 cggccttccc cgatgccgcc ccgtcaccgt cgtctcgcag cacacgtgca cggaacacga
      841 tcgactggcc accgcgggcg accggctcgc ccagcaggta tcgatcgccc aggatcaccc
      901 cgggtcgaag ctcgagcgat ggctcgtcgc gcgacacgcg ggtgaggtgc atggcgacga
      961 tggcaggatc gtcgattacc tcggtcgggt cttcgtccgc catggacagg agccaggcat
     1021 cgatgccgcg gctcaggtcg ccggcctcgg catcggtcag gcgaccgccg tcgtgcgctt
     1081 cgtggatgtc cgcctgcagt tgaccggcag tggcctcgtc ccagacccag gcgtcgcgca
     1141 cgtgcgccag cacgtcttcg ggcgcacgcg cgccgacgcg aagctccgcg accatgtagt
     1201 ccaggatgtc tctcggcgga atcatggctc cggctggaag gcgggggttc gctgcattat
     1261 cggcccgatc gtgcggtcgc cgccatcgac cgacaggcat gcgtggccgg gcctacccgg
     1321 caggtccacg cttggcgtca cgtacgacgt gctcgagcgc ttcgaggaag cgtgaacggt
     1381 cgcgcgggcc gaacggggcg gggccgccac cggtgccgta ggcgcgcagg tcggccatga
     1441 tttcccgcgt cgccagggcg ttgccgatgt tgtcgcccgt gaacggcctg cctgacggcc
     1501 ccagcacgcg cgcgcctttc ttcaggcagc gtgcggcgag cgggatgtct gcggtgacga
     1561 cgacgtctgc ttgcacgacc cgttcgacga tccagtcgtc ggcggcgtcg aaggaaccgg
     1621 acaccacttg caggcggagg cgcggcccgc gcggcgcctg cagccaggcg ttggacacgt
     1681 acgtcacggc gaggccgtgg cgttcggcaa ccttcgcgac ctctgccttg acggggcatg
     1741 cgtcggcatc tacgtagacg tgcggatcga gctgcatggc gtacagtcta gacgctgcgc
     1801 tcggctcgtc acgtgcagtc cgggcccgag gcagcaagag atccgcaaat aacaaaggag
     1861 agcgatgatc atgttccagg cgattgggtc gcggtcggtc ccggcaatcc tgccgatcgc
     1921 cctcgcgatc atgttcgcag catcgccggc cacggccatc gcccaggtga cggtattgct
     1981 gccgacggag ggtgatctcc aggccacgtt ggctttgccc ggcgacatac ggcgaggcca
     2041 ggaagcgtat gccgaatgtc aaacctgcca ccgcaaggat gcatcggggc gcgccaaatt
     2101 caacatgcca cggctgtccg ggcagcacgc ctcggtgctg atcaagcagc tgatggacat
     2161 ccgcagcggc ctccgcgtca atcccgacat gcgggagtac atgctcgact ccgacctgac
     2221 gctgcaggac tttgcggaca tggccgccta cctgcagtcc ctgccggtga cgggcaacat
     2281 cgcgaagggc ccgcccgagc tggtgccgcg cgggcaggcc ttgtacgcca gcgactgttc
     2341 cggctgccat ggcgagcacg gcgagggccg cggcgagctg ttcttcccca tgctcgcgtc
     2401 gcagcactac ggctacctgc tgcgcgaact cgacctcatc cttaccggcg agcgaggcaa
     2461 ctccaacccg gcgatgcccc cgctgctcaa ggactactcg gcggacgaca agcgcgcgtt
     2521 agccgcgtac ctggcgcaac tgcccgcgcc cgcaagaggc actgcgcctg ccgactagat
     2581 cgcgaatggc tggccccagt ggcggcagac gcgcgtcatc cctgcagcgg acgccctgcg
     2641 gcaaacttgc gccatgaatg acctgagacg ggtctggcgc gtgtcgcggg cgggctcgct
     2701 cgaccgcctg cggctccagg aagaatctct gccaccgccc ggacccgaag aggcgcgcgt
     2761 tcgcgtcgaa gccatcggcc tcaacttcgc ggacatcttt gcctgccagg ggttgtactc
     2821 ggccaccccg tccggatcgt tcgtgccggg actcgagtgt gccggggtcg tcgagtcact
     2881 cggaacggaa gcacggcgac tgtccgacct gcgtcccggc gaccgcgtga ttgcactcac
     2941 gcgcttcggt gcgtatgcaa cggcgctgaa cgtcggtgtc cagtatctgc gtcgagtacc
     3001 ggaaggctgg acgatggagc aggcggccgc gtgggccgtg cagggactca ccgcctggca
     3061 cggtctggtc gtcctcgggg ccgtgcaacg caacgacgta gtgctcgtgc agtcggcggc
     3121 tggcggcgtt ggcctgcttg cgctggacgt cgcggaggcg ctgggtgcgc gaccgatcgc
     3181 ggtggtcggt catgacgaca agggggagtt cctggtccgg cagcgcggca tcgcgccgtc
     3241 ttccgtcatc gtgcgggacc ggaaatcctt cggcccacgg gtcgacgctg cgacccgcgc
     3301 gctcggcgtc gacggtctcg actgcgtgct cgacgcggtg ctgggaccga cgttccggcc
     3361 ggcgttcgag cgactgcggc ccgagggccg gtacgtgctg tacggggcgg ccgatttcat
     3421 gtcgcaggcg tcacgaccag attacctcgt gctcggctgg cgatacctgc ggcgtccgcg
     3481 cctggatccg ctcgcgatga tttccgcgaa tcgcagcgtg ctggcgttca acctgatctg
     3541 gttgtgggag caggcagaaa gactgcccga ggcctacgcg gcgctccagc ggttgctgcc
     3601 gcgaccgccg cacgtgggcg caaggcttcc attcgaccgg gcgcccgagg ccatgcgtct
     3661 gctgcagagc ggaaagacga tcgggaaggt cgtgttgatg gtcgcgtgat cgttcagtgg
     3721 cgactgcaag gacgtgccta gcgctcgaac cgcgcgaggc cgcgcaggac cagcaggccg
     3781 aatgccgtca ccatgatggc gaggtagagc aatccgacgc cgactgcgat cccgatcccg
     3841 gcgaccagca acagcgatgc gcccgtcgtc agcccgacga tgccgttttc gcgggatcgg
     3901 aagatggtgc cggcgccgag gaacgacacg ccgatcacga tcgcctgcac catgcgtatc
     3961 gggtcggcct gcacggcgtg gttgcccgtg tcggccagca tctcgaacac caggtagtcg
     4021 gtcagtacca ccagcaatgc tgccgttccc gccgtgatgg cgtgggttcg aagtcccgcc
     4081 ggtcggttgg ccatctcgcg ttcgatgccg acgacggcgc cgagtgccgc ggcgacgatg
     4141 acctgcacgg ccgccgcaag ctgcggcatg aagtcctgtt gtacccagtc gaacatgcgc
     4201 atgactccac gttccggcac ggcaggtgcc gtcggtcgca tcctcgccaa tcctagtcag
     4261 cgcgggcgcg gaccgccaac gtctgggcgc cggcggctcg gccgcggagc aagaccggtt
     4321 tcgtggacgc cgcgggcgat ggccgcccgg ccgctgcgac ggactatcgc ggcttgtcga
     4381 agaatgcctg cacccgaagc agggaaggct tcatcacgct gtgccagtag ttccagtcgt
     4441 ggattccacg gctcacctcg taactgaacg ggaccccgtg ggccgacagg accgctgtca
     4501 gccgttcggc ataggggaag ctgaggtcgt cgctcctgcc cacgcgtatg tcgaggctcg
     4561 ccgcgacgcg tgcaccacgg cccagttcgc cgtcgaaccg gtcgaggaag tcgtggtcga
     4621 gatttccagg gctcagcacc attgcgtggc cgaagagctc gggatgccgg tacgcgaggt
     4681 agagggcgcc gaagccgccc atcgacatgc ccccgatcgc cgcggcgccc gcatcctggc
     4741 ggatggcgta ctcgcgcgcg atccaggggt agagctcctt gacgacgaac tcttcggaac
     4801 ggcccgtgta gaactcgtcg ggtgcacccg gcatcaccac caccatcggc ttcagggctc
     4861 ccgcggcgat gtaacgatcg aggtagtgct gcacgccgcc agtcagccac tggtccgcgt
     4921 tcatgccata gccgtgcagc aggatcagca ggggtagggc gtgcttcctc gtgtagcccg
     4981 gaggcgtgta gacgttgatc gcgcgcggct cgcctagcgc cttgctctcg aacgtccgct
     5041 gctcgatccg accctgagga atcccgcgca gcggttgcgc gtcgcggtcg tcgtccagca
     5101 cgaaccagga gcgctgcccc acgcctgcgt cattctcggg catcgaggcg tcgtccagcc
     5161 gtggctgtcc gtccaccatg aaaacgtact cgacgcgacc cggccgcagc ggcgcgttga
     5221 cgtagaagac gccggcaccg gtacggcgca tcgggatctg atcgcacttc cagccggtca
     5281 tgttgccgca gaggaaagcg gcgctcgcct cgggcgcctt cacctcgaag tgcacggtcc
     5341 gggtgtaggt ttcggcctcc accggctgtg cgcgaacgtg gactgccagc gaaatcatcg
     5401 ccagcaggag cgtctgcacg catttcctga acatggtgcg ctccggccgc ggtatccggc
     5461 cgggagaatt atcggtcccg aagccccggc acccaatgcg gagatatgcg gggcatggac
     5521 gtgtagcgga ccttccagct cgcggacccc tgcacgtttc cgtgcagggg ccgccaattc
     5581 gcgggtcgcc tcttcagaaa tccagcgaaa cgcggccgta gatgaaccgg cccgaccgac
     5641 cgaacggcgc atagttggag aacgacgtgt tgccggtcgt gttgcgattc ggttcgaacg
     5701 ggtccgggta ctcgtccagg acgttctccg cgccgatgga gacccggatg ttctccgtca
     5761 cgtcgaagcg gccttccagg tcgaccagcg tcttcgcgcc gagcgtgaag tcgagcgcat
     5821 ccgtagtccc cggatcgagc gcctcgccgt agtacgtcgc gcgcaacgtg gcgccgagcc
     5881 ggtcgccggt ccagttgacg atcgcggcga gcttctcctg tggcgtgcct tcctcgagcg
     5941 tcagcacgtt gatgcggtcg aacagcggcg gcggcggatc cagcgcctcc agttcccgcg
     6001 tctggggcac cttcgtcacg tcggtcgtcg tgtagttgcc ggccagcgtg aaatcgaacg
     6061 tcccggccgt gccggtctcc cacggccagt tgacgaccac gtcgacgccg gtcgtttccg
     6121 tgtccacgcc gttgatgaag aagcggccac cgccgacgcc gatgaagccg agtccctgca
     6181 ggtattcgcg cacgttggcc tgcgtgaggt tctcggagag cacgatgcga tcttcgatgt
     6241 cgatgcggta ggcgtcgatg gtgatgctga ccgccgcgag ccggaacacg gcgcccagcg
     6301 acaggttgat ggactcctcg gcgtcgagcg gctgcgcgcc gagcgcctgc gcgacggggt
     6361 cgctgacggg gaacgtcgtg atgtcgaacg gcacaccgtt gatgaagttc gtcgacgtgg
     6421 tggcgaagta ctgctgctgc agcgacggcg cgcggaatcc gttctgcacc gagccgcgca
     6481 acgcgaacga ctccgtgaag tcgtaacgcc ccgcgagctt gccggaaacg ttgttgccga
     6541 agtccgaata gtcctcgaac cggacggcgg ccgaaccgag gaacttctcc gtgaggttgg
     6601 cctcgaggtc gacgtacgca ccgaccgccg tgcgatcctc gtccacctcg ttcgagggtc
     6661 ggaacccggg gaagacctgc gcacctgact gcgtggggtt cccgttcggc agcagcacgc
     6721 cgccgttgcg atacgagtcc ggctcgccgg cgttgatctc gtagcgttcc tgccgtgcct
     6781 cgatgcccgc cgcgacgttc agcggcgacg cgagcgcgcc gagttccacc tgccgcacgc
     6841 cggagaagtt cagcaccagc tggtcatagg cgaaaccacc ggcgtcgaac ttcgtgccgc
     6901 tggccgggcc cagcgagcgg ttgagcgtgt tctcgatcgt gaagtccatc tcgttgcggc
     6961 catagacgag cgacgtgtcc atgtcccact caccgagcgc ccaggcgatg cccgccgcgg
     7021 cgctgtagtc gtcgacctcc ggcgcgatga tcggcaggaa cccgtcgggg tagatctcga
     7081 tgacgttgcg gtcgtcgagc gcgcgacgga agaagccggc cgaacgcgcc tcgcggttct
     7141 ggtagctcgc ccacccgtac aggtgcgcac cgttggcgag atcggaaccg gcatttgcaa
     7201 agaacgtcag ctggtcgagt tcgggctcac cgtaccaggc attgaagcga tcgaaatcct
     7261 gctcgcgcgg gtcgaacgcc gtgcccacca gcgggtactg ctggcggaag tcgtagccgc
     7321 cgcgttccgt gcggtcctgc tccttgtact cgccggcgag cgtcaggaac cccgagtcgc
     7381 cgaggccgag gcctttccag gccgaaacgg tgaacgtctc gccatcggag acgtcgcgct
     7441 cgatcttcga tggcgccgac caggtggcgc cggccggcaa cgggccggtg ggcgtgtcgt
     7501 aactcgattc gcgctggccg tacgagagcg tcacgtcgcc gccgtcgtcg ttctcccgca
     7561 ggcgcacgtt gatcacgccc gcgatcgcat ccgagccgta ctgcgccgac gcgccgtcgc
     7621 gcagcacttc gatggtctgc accgcggcgt tcgggatggt gttcaggtcc acggcggagg
     7681 cgccgcggcc gatggtgccg ttcacgttga ccagcgaggc cgcgtggcgc cgcttggaat
     7741 tgagcagcac gagcgtctgg tccggcgaca gtcctcgcag cgtcgcgggg cggatcgtgt
     7801 cggtcccgtc ggcgagaccc gggcgcggaa agttgtacga gggcaaggcg atcgacaagg
     7861 cctggttcag ttcgacgacg ccgacgtttt cgagcgctgc gcttgagacg acgtcgaccg
     7921 ggacggcggt ttcgagcgcc gagcgattct ccacgcgcgt gccggtgacg atgatctgct
     7981 cgaggtcccg gggtgtcgtc gtggcaggcg tttgttgcgc cgtggcaacg tggctgccag
     8041 tcaggccgcc gagcacggca atcaccgctg tccgaattcg gctctggttc gcttgcatga
     8101 gcaactccct ttgcgacccg gacatcgggt tcgtgccggc cgattacgca tgacgtgccg
     8161 cggcgggtca agcgcaggcc tgcagcggcc ctgcagcccg gctgttggaa aatcgcggca
     8221 ccacgctgtg gcgtccatgg gccgcgatgc agcgtcgatg cagcgtctct cgacgcgctg
     8281 cactttcggg ccgatgacgc agcggagagt tcttgtcctc ccgcccagcg cgcgtcgagc
     8341 aggtaggatc atccctaccg cgatgccggc gtgtttcacc acctgcgccc gcgcggttgc
     8401 cgccgcggcg ttatcgcgcg acactcggtc ggtgccacgc gtcgcgcgcc gtgcacggac
     8461 gtggcggcat cgtggtgcgc atgaattccg gcctgtgccg atccgcgtgc gacgaacgct
     8521 tggaaaccaa cctcccagga tgcgcaccac gtactgatgc cagccggatt ctccccaact
     8581 ccaaccacga tcgaccgctg ccggctgacg gaccacgaca tctacctgtt caagcagggc
     8641 acccacactc gactgcacga gaagatgggc gcgcacagcg tctccgacgc cggtcgcgct
     8701 ggcgtacatt ttgcggtgtg ggcgccgaat gcccgacggg tcagcgtcat cggcgatttc
     8761 aacgactggc gcgcgaacca cgatccgctg gccccacgca gcgacggctc cgggatctgg
     8821 gagggtttcg tcgccggagt cgggcacgga gccacgtaca agtaccgcat cgaaagccag
     8881 gggcgggcgc tggagaaggg tgatccgttc gcgcgctgct gggaggtccc accgcgcacg
     8941 gcctcgcggg tctggcaggg agagtacgag tggcgcgacc agcagtggat gcagcagcgc
     9001 ggcacgcgca acgcggcgga cgcaccgatg tcgatctacg aggtgcacct gggttcctgg
     9061 cgacgtcacc cggagaacgg ggcgcgctcg ttgacgtacc gcgagatggc ggagcaactg
     9121 ccggcctacg tcagcgccct gggtttcacg cacgtcgagt tcatgccgct gatggagcat
     9181 ccgttctatg gctcgtgggg ataccagacg acgggatact tcgccgcgac ctcgcgctac
     9241 ggcacccctg aagacctcaa gtacctggtc gacgcactcc acgggctggg catcggcgtc
     9301 atcttcgact gggtgccctc gcattttccc ggagatgcac acgggctggc ctgcttcgac
     9361 ggcacgcacc tgttcgagca ccaggaccgg cggcagggct tccatcccga gtggcagtcc
     9421 tggatcttca attacgggcg ccacgaggtg cgcgcttttc ttgcatcgag cgcgatgtac
     9481 tggctcgacg agttccacgc cgatgggctg cgggtcgatg ccgtggcttc gatgctgtac
     9541 ctggactacg ggcgcaagga cggcgagtgg attccgaatg cgcacggcgg ccgcgagaac
     9601 ctcgatgccg tcgagttcct gcgccagctg aacgaggcgg cctgccgcga ccagcccggg
     9661 acgaagacga tcgccgaaga gtcgaccgcc tggccggggg tcacgcggcc gacgagcgat
     9721 ggcggcctgg gcttcggcat gaagtggaac atggggtgga tgcacgacac gctcgagtac
     9781 ttcaagcgcg atccggtgca ccgcaggttc catcacgacg agctcaagtt cagcctctgg
     9841 tacgcgttca gcgaggactt cgtgctgccg ctgtcgcacg acgaggtcgt ctacgggaag
     9901 ggctcgctgc tgggccgcat gcccggcgac gagtggcagc ggttcgcaaa cctgcgcctg
     9961 ctgtacgggt acatgtggac gcaccccggc aagaagctgc tgtacatggg cggcgagttc
    10021 ggccagtggt cggagtggaa ccatgagtcg agcctcgact ggcagctgct ggagcggccc
    10081 ctgcaccgct cgctctcgca gtggatcggc gacctcaatc gcgtgctgcg ccagcgcggt
    10141 gcgctgcaca ctctggactt cgacgaacgg gggttccgct gggtcgacgc gtccgacgcg
    10201 ggacagagcg tcctggcata cctgcgtcgc ggcgcaggcg acgagtacgt gctggtggcg
    10261 tgcaacttca cgccggtgcc gcggcacaat catcggctcg gcgtgccggt cgagggtcgc
    10321 tggcgcgaga tcctgaacag cgacgcggcc ccgtacggtg gcagcggaca gggcaacctg
    10381 ggcggcgcca cgacggtgcc cgtggcggcg cacggttttc tgcagtcgat cgtcgtgacg
    10441 ttgccgccgc tcgccgtcgt catgttcgag cccgaatgaa tcggtcacgt cgaactggcc
    10501 cgagccatcg aagcgagtcg cgtgcgcagg aaggagtggt tgccatgacc aagaggggac
    10561 agaaggacac ggtgacgtca cgcgctgcac cggctgcacg caggtcggat tcgacgcccg
    10621 tcgcctcggt cgcgacgacg catcgcgcga cggcacacga cgtgcgtgtg cgcgcgatca
    10681 tcgaggacgt gcggccgcgg gtcgacggcg gccgcttccc gatcaagcgc gcggtcggtg
    10741 acgaggtctt cgtcgaagcg gacgtcttca cggacgggca cgacgtcccg cgcgccctcc
    10801 tgcggcatcg ccgcagcggc gagagcgact ggatcgaagt cgagatgact cacctggtca
    10861 acgatcgctg gcgcggccgc ttcgtcgtcg acgcgctcgg gcgctatgaa tacacggtag
    10921 tcgcctggac ggaccgcttc gcgacctggc gccgcgagtt cgagcgacgc gacgaggcgt
    10981 ccgacatcct gatcgcgctt cgcgccggcg tcggactgct cgagtcggcg gcggcgcgcg
    11041 ccggtggcga ggacgccgag cggttgcgcg cggtcgcgct cagctggacc gcggtcgagg
    11101 acccggacga agcacgtcgc gcgggcctcg cggacgacgt ccacgagctg gtcgggcgtc
    11161 acccggaccg tcggttcgag accgtgcacg agccggtcct ctgtgtcgtc gtcgacccgg
    11221 tcctcgcgcg ttgcggtgcc tggtacgagt tcttcccgcg ctcggcgagc gacgaccccg
    11281 gtcgccacgg cacgttccgc g
//
LOCUS       DASPFR010000236         2923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1655066, whole genome shotgun sequence.
ACCESSION   DASPFR010000236 DASPFR010000000
VERSION     DASPFR010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655066"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="VD737_06145"
     CDS             complement(<1..957)
                     /locus_tag="VD737_06145"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HXW10174.1"
                     /translation="MSVLAGVRVLDFGRYVAGPYCATLLGYLGAEVIRVEKREGGEDR
                     YIAPIAASGEGGVFFQTACNKKSLTLDPASPQGREVVARLVRSADVVVANLPPAGLRA
                     LGLDYASLCRWRPDVILVTQTSFGEAGPLADRGGFDGVGQAMSGAMYMTGTPGQPVKA
                     AAPYVDFSTAVLSAFGTLAALMHRAQTGEGQEVKATLLGTALAVFGSHLVEQGALGLD
                     RVPSGNRVQTSAPSDVFATRDGHVLVHTVGNGLFRRWARLMGDAERWTSDPRYQSDQS
                     RGDHSAEILARMSEWCAERSTEQALGELQAAGLPCGAVHTLRE"
     gene            complement(962..2122)
                     /locus_tag="VD737_06150"
     CDS             complement(962..2122)
                     /locus_tag="VD737_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXW10175.1"
                     /translation="MNFQLTDEQRSLQEAARTYARAKLPDVARECEESGHAPPKSLVQ
                     EFAGMGFLGINVPERYGGLGLGNVEALLVLEEFGKVSSAVAFPVFESCVGPVRAIERF
                     ASESLRQRVVPAVCRGEIQVAVSMSEPQAGSALTDLTTRAERKGDSVVLNGTKRWCSG
                     GGHAEGYVVYCRMSEDEGARGIGAVYVERDTPGVSFGRPESLMGFRGVPSSDIYFDNA
                     TVPADNVVVPAGGFKKLMEAFDLERCGNATMGLAQASGALEDVLEYVQEREQFGKPLL
                     EFQAVQLRLADMKMRVEAARLLIHRAASNAQDGLPSILDSSVAKCFANEIAREVCGHA
                     VQLMGGYGYSREYPMERRLRDAWGWGIAGGAIDIQKVNIAGAMVGRRFDQRR"
     gene            complement(2144..2461)
                     /locus_tag="VD737_06155"
     CDS             complement(2144..2461)
                     /locus_tag="VD737_06155"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HXW10176.1"
                     /translation="MSDRPYLAALTLTELAPGTMRCVTLADEPVLVVHAKGGTVHALH
                     NICSHAYARLDEGRLRGTRVICPLHGASFDVRTGAVLGAPATVPLKHYPVRVVDGRIE
                     VQV"
     gene            2597..2893
                     /locus_tag="VD737_06160"
     CDS             2597..2893
                     /locus_tag="VD737_06160"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HXW10177.1"
                     /translation="MPRITFISRMTVKPGRERDFVEACNELTASVKEHEPGVIYYEFF
                     KLREPRRYAVLESFPSEEAEHAHMNSTWLAAVGPRIVDCLDGTWEREYLDPFEP"
BASE COUNT          468 a         1045 c          974 g          436 t
ORIGIN      
        1 ctcgcgcaag gtgtgcacgg cgccgcaggg cagccccgcc gcctgcagtt caccaagcgc
       61 ctgttcggtc gagcgctccg cacaccactc gctcatgcga gcgaggatct cggcgctatg
      121 gtcgccgcgg gactggtcgg actgataacg cgggtcgctg gtccagcgct ccgcgtcgcc
      181 catcagccgg gcccagcggc ggaagaggcc gttgccgacc gtgtgcacca ggacgtggcc
      241 gtcgcgggtc gcaaacacgt ccgacggcgc cgaggtctgc acgcgattgc ccgagggcac
      301 gcggtcgagg cccagcgcgc cctgctcgac gaggtgcgag ccgaacaccg ccagcgccgt
      361 gccgagcagc gtcgccttca cttcctggcc ttcgccggtc tgcgcgcggt gcatcagcgc
      421 cgcgagcgtg ccgaacgccg acagcaccgc ggtcgaaaaa tccacgtacg gcgccgcggc
      481 cttgaccggc tggccgggcg tgccggtcat gtacatggca ccggacatcg cctgcccgac
      541 gccgtcgaag ccaccgcgat cggccagcgg cccggcctcg ccgaacgacg tctgcgtcac
      601 caggatcacg tcggggcgcc atcgacacag cgacgcgtaa tcgaggccca gcgcgcgcag
      661 tcctgccggc ggcaggttcg cgacgaccac gtcggcgctg cgcacgagcc gcgccaccac
      721 ctcgcgcccc tggggtgacg cgggatccag cgtcagcgat ttcttgttgc aggccgtctg
      781 gaagaagacg ccaccttcgc cgctcgccgc gatcggcgcg atgtagcggt cctcgccgcc
      841 ctcgcgcttt tcgacgcgga tgacctcggc gcccaggtat ccgagcagcg tcgcgcagta
      901 cggaccggcg acgtaccgcc cgaagtcgag cacgcgcacg ccggcgagca cgctcaccgg
      961 gtcagcgcct ctggtcgaag cgacgcccga ccatcgcgcc ggcgatgttg accttctgga
     1021 tgtcgatcgc gccgccggcg atgccccagc cccacgcgtc gcgcaggcgc cgctccatcg
     1081 ggtactcgcg cgaatagccg taaccgccca tcagttgcac ggcgtggccg cagacttcgc
     1141 gtgcgatctc gttggcgaaa cacttggcga ccgaggagtc gaggatgctc ggcaggccgt
     1201 cctgcgcgtt cgacgctgca cggtggatca gcagcctggc cgcctcgacg cgcatcttca
     1261 tgtcggcgag tcgcagttgc acggcctgga actccagcag cggcttgccg aactgctcgc
     1321 gctcctgtac gtattcgagc acgtcctcga gcgcgccgga ggcctgcgca agtcccatgg
     1381 tcgcgttgcc gcagcgctcg aggtcgaacg cttccatcag tttcttgaaa ccgcccgccg
     1441 gcacgacgac gttgtcggcc ggcacggtcg cgttgtcgaa gtagatgtcg gaggagggca
     1501 cgccgcgaaa gcccatcagg ctctcggggc gaccgaacga gacacccggc gtgtcccttt
     1561 cgacgtagac cgcgccgatg ccgcgcgccc cctcgtcttc cgacatgcgg caatagacga
     1621 cgtaaccctc cgcgtggccg ccgccggaac accagcgctt ggtgccgttc aggaccacgg
     1681 agtcaccctt gcgttcggcg cgcgtcgtca ggtcggtcag tgccgagccg gcctgtggct
     1741 cggacatcga cacggcgacc tggatctcgc ccctgcacac ggccggcacg acgcgctgcc
     1801 gcagcgattc ggacgcgaag cgctcgatgg cgcgcacggg tccgacgcag gactcgaaca
     1861 ccgggaaggc gacggccgag gagaccttgc cgaactcctc gagcacgagc agcgcctcga
     1921 cgttgccgag cccgaggccg ccgtagcgct cgggcacgtt gatgccgagg aagcccatcc
     1981 cggcgaactc ctggacgagt gacttcggcg gcgcgtggcc cgattcctcg cattcgcgcg
     2041 cgacgtctgg cagcttcgcg cgcgcgtagg tacgagcggc ttcctgcagc gagcgttgtt
     2101 cgtcggtcag ctggaaattc acgcgcaggc tcctaagcgg gcgtcagacc tggacctcga
     2161 tgcggccgtc gacgacgcgc accggataat gcttgagcgg cacggtcgcg ggggccccga
     2221 gcacggcgcc cgtgcgcacg tcgaagctgg ccccgtgcag cggacagatc acccgagtac
     2281 cgcgcagccg gccttcgtcg agcctggcgt acgcgtggct gcagatgttg tgaagcgcgt
     2341 gcaccgtgcc gcccttcgcg tggaccacga gcaccggctc gtcggcgagc gtcacgcaac
     2401 gcatcgtccc gggcgcgagc tcggtcaggg tcagtgcggc aaggtagggt cgatcggaca
     2461 cgctgtgctc caccgcggca ggcgcggcga ccgccgcaat gtaccgtaac tcgtgtaaaa
     2521 agaaacaggc ttgactgttt tattccggcg aagccgggtg ctaccatcga cccgccttcg
     2581 caacggggac gaccgcatgc cgcgcatcac tttcatttca cggatgaccg tcaagcccgg
     2641 gcgcgagcgg gatttcgtcg aggcctgcaa cgagctgacc gcaagcgtca aagaacacga
     2701 gcccggggtg atctactacg agttcttcaa gctgcgcgag ccgcggcgtt atgccgtgct
     2761 ggaatctttc ccgagcgaag aggccgaaca cgcgcacatg aactcgacct ggctcgccgc
     2821 cgtcggcccg cgcatcgtcg attgcctcga cggcacctgg gagcgcgagt acctggaccc
     2881 gttcgagccc tgaccctgcc cgtcgtcgcc gtcgaggacc cat
//
LOCUS       DASPFR010000237         3514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1776192, whole genome shotgun sequence.
ACCESSION   DASPFR010000237 DASPFR010000000
VERSION     DASPFR010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776192"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VD737_06165"
     CDS             <1..560
                     /locus_tag="VD737_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HXW10178.1"
                     /translation="GVDYRQVVVDREASTIRFLRPGMRIDLGGIAKGWAVDRGVEILR
                     RHGLAHGMVNAGGDTAFLGDRRGKPWIVGVQDPRDPGRVVARIPLADEAISTSGDYER
                     YFEQDGVRYHHILVPGTGKSPGAVQSVTLIGANATRTDGLTKTVFILGVERGLEFVRT
                     LGDVEAVIVDATGCVHCSAGLQPPA"
     regulatory      682..765
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            853..1284
                     /locus_tag="VD737_06170"
     CDS             853..1284
                     /locus_tag="VD737_06170"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXW10179.1"
                     /translation="MSTVAHGVPSPAPDFVLKATDGRNLRLAEFRGDPVIVTFWGSWC
                     GECRETLVALDAVSERTSTPVLGVNLDGSVERAASVATSLELHFPTLVDSKKSVARAY
                     DVVRLPLTLLIDRHGAVRATWEGVAMPADDLTRRLDALRQE"
     gene            1290..1835
                     /locus_tag="VD737_06175"
     CDS             1290..1835
                     /locus_tag="VD737_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10180.1"
                     /translation="MTARPRRTSMRVAWFALALLLPGLAGAEAPVPDTKSVDQDVQSL
                     KKQLIDLNRDLFKLEEELLYPASTQVAVFLSVDVGTFFALDAVTLEIDDKEVANYLYT
                     DREVQALHRGGVQKLYLGNLKAGEHELVAFFTGKGPHDRDYRRGTTLKFDKAVGAKYV
                     ELRISDREASLQPEFVVRQWE"
     gene            1868..>3514
                     /locus_tag="VD737_06180"
     CDS             1868..>3514
                     /locus_tag="VD737_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXW10181.1"
                     /translation="MPRLTRTARLYLVCAMLMLGAAAAWTARADEVDRDAAPPTRVRD
                     LDYGDVLFHFLQDDYFAAIVRLESHRDFGRMQSHAAEAELLAGGLYLSLGLHTEATRI
                     FDRLLAGPLAPSVRDRARFYLARIGHQRGYHEEALRNLGRIGAPLEGKLEPERRLLES
                     NVLMALGRYDEAAARLGQWTDTTGWSHFARFNLGVALVRAGDTPRGRTYLEQVGTLDP
                     RTEEQAALRDRANLALGFAVLQQHGGDEAVTALSRVRLDGPFTNRALLALGWAETNAG
                     RHERALVPWLELQQRTLLDASVQESLLAVPYAYVRLASNGQASQHYRSAVDAYARETR
                     RIDESIAAIRADGFLDALLDPVPREDNVDWFWQLESLPDAPQTRYLYPLLASHEFQEG
                     LKNYRDLRLMQANLSRWRDALEAFDAMIDARDRATGVREPRREAALQALDLDALAKRH
                     AALSTRLAAVEASRDVAGLATDAERTRWESLAGIDDRLTALPAGPAREELAERARLLR
                     GVVVWQLDREFKYRLANARRDLADASAALAQARQQAAAIAA"
BASE COUNT          531 a         1238 c         1220 g          525 t
ORIGIN      
        1 cgggcgtgga ttaccggcag gtcgtggtcg accgcgaggc cagcaccatc cgtttcctgc
       61 ggccgggcat gcgcatcgac ctgggcggga tcgcgaaggg ctgggcggtc gatcgcggcg
      121 tcgagatcct ccgtcggcac ggcctcgcgc acggcatggt caacgcgggc ggcgatacgg
      181 ccttcctcgg cgaccgtcgc ggcaagccgt ggatcgtggg cgtgcaggac ccgcgcgacc
      241 ccgggcgcgt cgtcgcaagg atcccgctcg ccgacgaggc gatctcgacc tccggcgact
      301 acgaacgcta tttcgagcag gacggcgtgc gctaccacca catcctggtg cccgggacgg
      361 gcaagtcgcc gggcgcggtc cagagcgtga ccctgatcgg cgccaacgcg acccgtaccg
      421 acgggctgac caagacggtg ttcatcctgg gcgtcgaacg tgggctcgag ttcgtgcgga
      481 cgctcggtga cgtcgaagcc gtgatcgtgg acgccacggg atgcgtgcac tgttccgcgg
      541 gcctgcagcc acccgcgtaa ccgcaaggtt cccggagtcc ggagggcgcc acaaacccca
      601 ttttgtgatc cattgcttgc ctttggggaa atccgacccg ttgcgttgac atgggcgcgg
      661 gccccagaca acgatgcttg ccactgacaa gggcaaaccc gtcgaaaggc ggggacgcaa
      721 agccaccggt cttcgggacg tacccatgac agcggggttg ccagggtgca acgcaggaca
      781 caacgagaac gtgccccgcc gaggggccat gctgcacgcg tcgcgctgag cgtcgcgttc
      841 gcggcctgtg cgttgtcgac cgttgcccac ggcgtgccgt cgcccgctcc cgatttcgtc
      901 ctcaaggcga ccgacggtcg caacctgcga cttgccgagt ttcgcggcga ccccgtgatc
      961 gtcacgttct ggggcagctg gtgcggcgag tgccgcgaga ccctggtcgc gctcgatgcc
     1021 gtgtccgaac gaacctcgac accggtgctc ggcgtcaacc tcgacggcag cgtcgagcgc
     1081 gccgcgtcgg tcgcgacgtc gctcgaattg cattttccga cactggtcga ttcgaagaag
     1141 tcggtggcgc gcgcctacga cgtcgtgcgt ctcccgctca cgctgctgat cgaccgccac
     1201 ggggcggtgc gcgccacgtg ggaaggcgtt gcgatgccgg ccgacgacct gacccgccgt
     1261 ctcgacgcat tgcgacagga atgaaggcca tgaccgcccg ccctcgacga acttccatgc
     1321 gcgtggcgtg gttcgcgctc gccttgctgc tgccagggct cgcgggtgca gaggcgccgg
     1381 tgcccgacac gaagtccgtc gaccaggacg tgcagtcgct caagaagcag ttgatcgacc
     1441 tgaaccgcga cctgttcaag ctcgaggagg agctgctgta cccggccagc acgcaggtgg
     1501 cggtgttcct gtcggtcgac gtaggcactt tcttcgcgct cgacgccgtc acgctcgaaa
     1561 tcgacgacaa ggaggtcgcg aactacctgt acaccgaccg cgaagtgcag gcgctgcacc
     1621 gcggcggcgt gcagaagctg tacctcggca acctgaaggc gggcgagcac gagctggtcg
     1681 cgttcttcac gggcaaggga ccgcacgatc gcgactaccg ccgcggcacg accctcaagt
     1741 tcgacaaggc ggtcggtgcc aagtacgtcg agctgcggat cagcgaccgc gaggcctcgc
     1801 tgcagcccga gttcgtcgtg cggcagtggg agtaggtcgc aggtctgcca ccgacggtcg
     1861 ttccgacatg ccgcgcctga cccgcaccgc ccgcctgtac ctggtgtgcg ccatgctgat
     1921 gctcggcgcg gccgccgcct ggacggcccg tgccgacgag gtcgatcgcg acgcggcccc
     1981 gcccacgcgc gtgcgcgacc tcgattacgg cgacgtgctg ttccacttcc tccaggacga
     2041 ctatttcgcg gccatcgtcc ggctcgagag ccatcgcgat ttcggccgca tgcagtcgca
     2101 cgcggccgag gcggagttgc tcgccggcgg gctgtacctg tcgctcggcc tgcacaccga
     2161 ggccacccgg atcttcgacc ggctgctcgc cgggcccctg gcaccctcgg tgcgcgaccg
     2221 cgcccgtttc tacctggcgc gcatcggcca ccagcgcggc taccacgagg aggcgctgcg
     2281 gaacctgggc cgcatcggcg cgccgctcga aggcaagctc gaacccgaac ggcgcctgct
     2341 cgagtcgaac gtactgatgg cgctgggccg ctacgacgag gcggcggcgc ggctcgggca
     2401 atggaccgac acgaccggct ggtcccattt cgcccgcttc aacctcggcg tcgcactggt
     2461 gcgcgcgggc gacacgccgc gcgggcgtac gtacctggag caagtcggca cgctcgatcc
     2521 gaggaccgag gagcaggccg cgcttcgcga ccgcgccaac ctcgcgctcg gtttcgcggt
     2581 gctgcagcag cacggcggcg acgaggcggt aaccgcgctc agtcgcgtgc gcctcgatgg
     2641 gccgttcacc aaccgcgcac tgctcgcgct cggctgggcc gagaccaacg ccgggcgtca
     2701 cgaacgtgcg ctcgtgccgt ggctcgagtt gcagcagcgc acgctgctcg acgcgtccgt
     2761 gcaggagtcg ctgctcgcgg tgccgtacgc ttacgtcagg ctggcgtcga acgggcaggc
     2821 gtcgcagcac taccgttccg cggtcgacgc ctacgctcgc gagacccggc gcatcgacga
     2881 atcgatcgcg gcgatccggg cggacggctt cctcgacgcg ctcctcgatc cggtcccgcg
     2941 cgaagacaac gtcgactggt tctggcagct cgaatcgctg ccggacgcac cgcagacgcg
     3001 ctacctgtac ccgctgctcg cgtcgcacga gttccaggaa ggcttgaaga actatcgtga
     3061 cctgcgcctg atgcaggcca acctctcccg ctggcgcgac gcgctcgagg cgttcgacgc
     3121 catgatcgac gcgcgcgacc gcgcgaccgg cgtgcgcgag ccgcgacgag aggcggcgct
     3181 gcaggcgctc gatctcgacg cgctcgcgaa gcgccacgcg gccctgtcca cgcggctggc
     3241 cgcggtcgag gcgtcgcgcg acgtcgccgg gctggcgacc gatgccgagc gcacccgatg
     3301 ggagtcgctg gcggggatcg acgaccggtt gacggccctg ccggccggcc ccgcgcgaga
     3361 agagctggcc gaacgcgcgc gcctgttgcg tggcgtcgtg gtgtggcagc tggaccgcga
     3421 gttcaagtat cgactcgcca acgcgcgacg cgacctcgcg gacgcttcgg cggcgctcgc
     3481 acaggcccgg cagcaggccg cggccatcgc ggcc
//
LOCUS       DASPFR010000238         6113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_801575, whole genome shotgun sequence.
ACCESSION   DASPFR010000238 DASPFR010000000
VERSION     DASPFR010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801575"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1725
                     /gene="glgB"
                     /locus_tag="VD737_06185"
     CDS             <1..1725
                     /gene="glgB"
                     /locus_tag="VD737_06185"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF003811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HXW10182.1"
                     /translation="DGRRHPMQARGGSGIWELFVPDVGPGTLYKYEVRTRTGRTLLKS
                     DPYGFAMELRPGNASIVASLEGFEWHDQAWLEARAQANHTERPVNVYEVHPGSWRRAY
                     DRTPPFLNWRELADELIPYVKEQGYTHVELMGVAEHPFDGSWGYQVVGYFAPSSRFGS
                     PHDFMHFVDRCHAAGVGVLIDWVPAHFPRDENGLATFDGTALYEHADPRLGEHADWGT
                     KIFNYGRHEVRNFLVANALYWLDRYHVDGLRVDAVASMLYLDYSRKPGEWLPNRHGGR
                     ENLEAIDFLRQLNTAVGRYFPGVMTFAEESTAFPGVTQPAHLGGLGFHFKWNMGWMND
                     TLRYVEHDPVHRRYHHSLVTFSFVYAWSEKYVLPISHDEVVHGKKSLLAKMPGDEWQQ
                     RANYRWYRAFMTAHPGKKLLFMGTEFGQWQEWRDEHSLDWHLLEQPAHRALLDLDRDL
                     NCIYRDYPQLHASDADPAGFAWVDLHNGDQSVFAFLRRCPQQGERPALLCVFNCTPVP
                     RDDYWLGVPAAGSWRKILDTDAARYGGSGYEQRDRFTSQATPVQGYPCHVRLNLPPLS
                     AVFLELER"
     gene            1737..2675
                     /locus_tag="VD737_06190"
     CDS             1737..2675
                     /locus_tag="VD737_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXW10183.1"
                     /translation="METRPYGDSGLRVPVLGFGAMQVGDPTLPEGHVERLLHHVLDSG
                     LTLLDTARSYGLAEERIGRHLAGRRDEFVLSTKVGYGVPGIPDWTYECVLAGVDAARD
                     RLRTDVIDIVHLHSCGLATLESGDVTRALDRCRELGKIRVAAYSGDGDALRFAATSCG
                     APGEPGRPGEVARSAGEGFRGLQSSVNLCDQQALPLLEAARSRGVGTLAKRSLAGRPW
                     QGTASGDPVHDEYRRRFVALRARLSLEVADWEELALRFAAFSPGVDCVIVGGIDAANV
                     DRNVATVARGPLMLQARSAVTDAWDAEGGPHWPGLV"
     gene            2704..4809
                     /gene="glgX"
                     /locus_tag="VD737_06195"
     CDS             2704..4809
                     /gene="glgX"
                     /locus_tag="VD737_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HXW10184.1"
                     /translation="MNDSAAPAPGLEAGSPQPLGAYWTGRGTNFAVFSAHAERVELCL
                     FDESGRQEVARLDLPARTGNLWHGFLPARFGGPGLKYGYRVHGPYDPARGHRFNPNKL
                     LLDPCAQSVRGEFTWHPALLGGLRGHDERPSRDDSAPYVPRCEVVDGTFDWGKVRAPN
                     VPWRDTIVYETHVKGYTQLHPEVPPEWRGKYLGLTHPAVIDHLQRLGVTTIELMPVQA
                     FVSEQFLHERGLRNYWGYNPIAWFAPEPSYAVEDAVVEFKTMVRALHAAGFEVVLDVV
                     FNHTAEGNEHGPTLNLRGFDNAVYYRLLQDRAHYVNHSGCGNTINFDENAVGALVIDC
                     LRYWATEMRVDGFRFDLATILGRVAHGYDRNAPLFAAMRADPELAYLKMIAEPWDVGP
                     GGYQLGHFPSGWSEWNDRFRDTVRAFWRGDRPVVGTFAERFAGSSDLFRGGGRKPTAS
                     VNYVAAHDGYTLRDTVSYVHRHNEANLEHNRDGHAHNLSANYGVEGPTDDPEVVAVRE
                     RQSRNLLASVFLSQGVPMLLAGDEFGRTQRGNNNAYCQDNELSWVDWSLLEQNASLVE
                     FVRQLIDLRKSRLWLRRDTFLKGTRRGAHAKDVTWLHPSGREMTGVDWNDSNLSSIAV
                     HMNGAPSRNSNTGDLLVVFNADEAPMTMTLPAPPEGSAWAVVFDTAVAHPTSARFLQR
                     NETLTIEQRSTVLLESQTA"
     gene            5011..>6113
                     /locus_tag="VD737_06200"
     CDS             5011..>6113
                     /locus_tag="VD737_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10185.1"
                     /translation="MSRRSFLSLSKLATTLLVASTVLAGCGSGSNGVADTGGGGGGGV
                     TVNNPVTVTETFDFRAPLGVFTTGSPPIHARFLGGHADDNGAWIVREGETATINFATP
                     ADKVVFDAIDNYTAAVAAARQAKTTRPGAQKFDVATYVRGSVYNDWIADAGNQLVGSG
                     EVIEVSLPLTAGDYQFKVADAGWASINCGGGDSDVVTLGAALTLACGTNPPNLNLSIT
                     EDANYKFSVDATDPDAPVLTVTKVTSGGGGGGGGGGGEPRACGLTDQGLDNTAPFGVE
                     VYIRGSVAADWAAAPENKLFQFGGNLLKRELQVQAGAAQFKVADAGWASINCGAGDSD
                     TLTLGAAFTMACGTNPPNVNVEFPAAGCYEFAV"
BASE COUNT          959 a         2144 c         2089 g          921 t
ORIGIN      
        1 gacggccgca ggcacccgat gcaggcccgg ggcggttcgg gcatctggga gctgttcgtg
       61 cccgacgtcg ggccgggcac gctgtacaag tacgaggtcc gcacgcgcac cggccgcacg
      121 ctgctgaagt ccgacccgta cgggttcgcc atggagttgc gtcccggcaa cgcatcgatc
      181 gtcgcctcgc tcgaggggtt cgagtggcac gaccaggcat ggctcgaggc gcgggcgcag
      241 gccaaccaca ccgagcgtcc ggtcaacgtc tacgaagtgc atcccggcag ctggcgtcgc
      301 gcctacgatc gcacgccccc gttcctgaac tggcgcgagc tcgccgatga actcattccg
      361 tacgtcaagg agcagggcta cacgcacgtc gaactgatgg gcgtcgccga gcacccgttc
      421 gacgggtcgt ggggctacca ggtcgtgggg tatttcgcgc cgagctcgag gttcggttcg
      481 ccccacgact tcatgcactt cgtggaccgg tgccacgcgg ctggcgtcgg ggtgctgatc
      541 gactgggtgc ccgcgcactt cccgcgcgac gagaacgggc tcgccacgtt cgacggcacg
      601 gcgctctacg agcacgcgga cccccgcctc ggcgagcacg ccgactgggg caccaagatc
      661 ttcaactacg gccggcacga ggtgcgcaac ttcctggtgg ccaatgccct gtactggctc
      721 gaccgctatc acgtcgacgg gctgcgcgtg gacgcggtcg cctcgatgct ctacctcgac
      781 tacagccgca agccggggga gtggctaccg aaccggcacg gcggccgcga gaacctcgag
      841 gccatcgact tcctgcgaca gctgaacacg gcggtgggtc gctacttccc gggcgtaatg
      901 acgtttgccg aggagtcgac ggccttcccg ggcgtgacgc agccggcgca cctcggcggg
      961 ctcggcttcc acttcaagtg gaacatgggc tggatgaacg acacgctgcg ctacgtcgag
     1021 cacgatccgg tgcaccgtcg ctatcaccac tccctggtga cgttctcgtt cgtgtacgcg
     1081 tggtccgaga aatacgtgct gccgatctcc catgacgagg tcgtgcacgg caagaagtcg
     1141 ctgctcgcca agatgccggg cgacgagtgg cagcagcgcg ccaattaccg ctggtatcgc
     1201 gcgttcatga cggcgcaccc cggcaagaag ctgctgttca tgggcaccga gttcggccag
     1261 tggcaggagt ggcgcgacga gcactcgctc gactggcacc tgctcgagca gccggcacac
     1321 cgggcgttgc tcgacctgga ccgcgacctc aactgcatct accgcgacta cccgcagctc
     1381 cacgcgagcg atgcagaccc ggccggcttc gcctgggtcg acctgcacaa cggcgaccag
     1441 agcgtgttcg cgttcctgcg ccgctgcccg cagcagggcg aacgcccggc cctgctctgt
     1501 gtgttcaact gcacgccggt gccgcgcgac gactactggc tgggcgtgcc ggcggccggc
     1561 tcgtggcgca agatcctgga caccgacgct gcccgctatg gcggctcggg ctacgagcag
     1621 cgcgaccggt tcacgtcgca ggcgacgccc gtgcagggct acccgtgcca cgtgcggttg
     1681 aacctgccgc cgctctcggc cgtcttcctc gagctcgaac gctgaagccc gggccaatgg
     1741 agacgcggcc ttacggcgac agcgggctgc gcgtgccggt gctcggcttc ggcgcgatgc
     1801 aggtcggcga ccccacgctc ccggaagggc acgtggagcg cctgttgcac cacgtcctcg
     1861 attccgggct cacgctgctc gacaccgcgc gctcgtacgg gctcgccgag gagcgcatcg
     1921 ggcgtcacct cgcgggccgg cgcgacgagt tcgtcctgtc cacgaaggtg ggctacgggg
     1981 tgcctgggat tcccgactgg acctacgagt gcgtgctcgc cggcgtcgac gcggcgcgcg
     2041 accgtctgcg caccgacgtg atcgacatcg tgcacctgca ctcgtgcggc ctcgccacgc
     2101 tcgaatcggg cgacgtcacc cgcgcgctgg accgctgtcg cgaactcggc aagatccgtg
     2161 tcgcggcgta ctcgggcgac ggcgatgcgt tgcgctttgc cgccacctct tgcggcgcac
     2221 cgggagagcc cggcaggccc ggagaggtcg cgcgcagcgc gggtgagggc ttccgtggcc
     2281 tgcaatcgtc cgtcaatctc tgcgaccagc aggcgttgcc gctgctcgag gcggcgcggt
     2341 cgcgcggcgt cggcacgctc gccaagcgtt cgctcgcggg gcggccctgg caagggaccg
     2401 cgagcggcga cccggtccac gacgagtacc gccgccgctt cgtcgcgctg cgggcccggc
     2461 tgtcgctcga ggtcgcggac tgggaggagc tcgcgctgcg attcgcggct ttttcgccgg
     2521 gcgtcgactg cgtgatcgtg ggcggtatcg acgccgccaa tgtggatcga aacgtcgcga
     2581 ccgtcgcgcg cggtccgctc atgctgcagg cccgctccgc cgtgaccgac gcctgggacg
     2641 ccgagggcgg cccccactgg ccgggcctcg tctgaaatgt cgtccatcga actgaaccca
     2701 cccgtgaacg attccgccgc gccggcgcct ggcctcgaag ccggttcacc acagccgctc
     2761 ggcgcctact ggaccgggcg cggcaccaat ttcgcggtct tctcagcgca cgcggagagg
     2821 gtcgagctgt gcctgttcga cgagtcgggg cgccaggaag tcgcgcggct ggacctgccc
     2881 gcgcgcaccg gcaacctgtg gcacgggttt ctcccagccc ggtttggcgg gcctggcctc
     2941 aagtacggct accgtgtcca cgggccgtac gacccggccc gcggccaccg cttcaacccg
     3001 aacaagctgc tgctcgaccc gtgtgcacag tcggtgcgcg gcgagttcac gtggcatccg
     3061 gcgctgctgg gcggcctgcg cggccacgac gaacggccct cgcgcgacga cagcgcgccg
     3121 tacgtgccac gttgcgaagt cgtcgacggg acgttcgact ggggcaaggt gcgcgcgccg
     3181 aacgtcccat ggcgcgacac gatcgtctat gaaacccacg tcaagggcta cacgcagctg
     3241 cacccggagg tgccgcccga gtggcgcggc aagtacctgg gactcacgca tcccgccgtg
     3301 atcgaccacc tgcagcgcct gggcgtgacc acgatcgagc tgatgccggt gcaggctttc
     3361 gtgagcgagc agttcctgca cgaacgcggc ctgcgcaact actggggcta caacccgatc
     3421 gcgtggttcg cgcccgagcc gagctatgcg gtcgaggatg ccgtcgtcga gttcaagacc
     3481 atggtgcgcg cgctgcacgc ggccggtttc gaagtcgtac tcgacgtcgt gttcaaccac
     3541 accgccgagg gtaacgagca cggcccgacg ctgaacctgc gcgggttcga caacgccgtg
     3601 tactaccggc tgctgcagga ccgcgcgcac tacgtgaacc actccggctg cggcaacacg
     3661 atcaacttcg acgagaacgc ggtcggcgcg ctcgtcatcg attgcctgcg gtactgggcg
     3721 accgagatgc gcgtcgacgg cttccgcttc gacctcgcga cgatcctcgg ccgcgtcgcg
     3781 catggctacg atcgcaacgc gccgctgttc gcggccatgc gcgccgaccc ggaactcgcc
     3841 tacctgaaga tgatcgccga gccctgggac gtcggtcccg gcggctacca gctcgggcac
     3901 ttcccgtccg ggtggtcgga gtggaacgac cgattccgcg acacggtgcg ggcgttctgg
     3961 aggggcgacc gccccgtcgt cggcaccttc gccgaacggt tcgcgggctc gagcgacctg
     4021 tttcgcggcg gcggacgcaa gccgacggcg agcgtcaact acgtggccgc gcacgacggg
     4081 tacacgctgc gcgacacggt gtcgtacgtt caccgccaca acgaggcgaa cctcgagcac
     4141 aaccgcgacg gccacgcgca caatctgagc gcgaattacg gcgtcgaggg accgaccgac
     4201 gatccggagg tggtggcggt gcgcgaacgc cagtcgcgca acctgctcgc ttcggtgttc
     4261 ctgtcgcagg gcgtaccgat gctgctggcg ggcgacgagt tcggccgcac ccagcgcggc
     4321 aacaacaacg cgtattgcca ggacaacgag ttgagctggg tcgactggtc gctgctcgag
     4381 cagaacgcct cgctggtcga gttcgtgcga cagctgatcg acctgcgcaa gtcacggctc
     4441 tggctgcgtc gggacacttt cctgaagggc acgcgccgcg gcgcgcacgc caaggacgtc
     4501 acctggctgc acccgagcgg ccgcgagatg acgggcgtcg actggaatga ctccaacctg
     4561 agctcgatcg ccgtccacat gaatggcgcg ccgtccagga actcgaacac cggcgacctg
     4621 ctggtcgtgt tcaacgccga cgaggcgccg atgaccatga cgctgccggc gccgccggag
     4681 ggcagcgcct gggccgtggt cttcgacacc gcggtcgcgc atccgacgag cgcgcgtttc
     4741 ttgcagcgca acgaaacact gacgatcgag cagcgcagca cggtgctgct cgaatcccag
     4801 acggcgtaac gtccgaacag gcatcgatgt gcccggcgcc cacgcgtcgg gcgctgcata
     4861 actgcgcttg cggtgtccca cgttttggtg gcatcgtcgc cgcgaaccgg actccgccct
     4921 caagcgcaga cgaagccggt actcaaggat caggggaggc gggcgtgccc gcccacgaat
     4981 aaagccgcat gacctcagga gtgtgtgtcc atgtcgcgcc gttccttcct tagcctgtcc
     5041 aagctcgcca cgacgctcct ggtcgcatcg accgtcctcg ccggttgcgg cagcggatcg
     5101 aacggagtcg ccgacaccgg aggtggcggc ggtggcggcg tgaccgtcaa caacccggtc
     5161 acggtcaccg agacgttcga cttccgcgcg ccgctcggcg tgttcacgac tgggtcgccg
     5221 ccgatccacg cgcgcttcct cggcgggcac gccgacgaca acggggcgtg gatcgtccgt
     5281 gagggcgaga ccgcgacgat caatttcgcg accccggcgg acaaggtcgt cttcgacgcg
     5341 atcgacaact acacggccgc cgtcgcggcg gcacggcagg ccaagacgac gcggcccggc
     5401 gcgcagaagt tcgacgtcgc gacgtacgtg cgcggcagcg tctacaacga ctggatcgcc
     5461 gacgccggca accagctggt cggatcgggc gaggtcatcg aagtctcgtt gccgctcacg
     5521 gccggcgact accagttcaa ggtcgccgac gccggctggg cctcgatcaa ttgcggcggc
     5581 ggggacagcg acgtcgtcac gctcggcgca gccctcacgc tggcctgcgg caccaatccg
     5641 ccgaacctga acctgtcgat caccgaggac gccaactaca agttcagcgt cgacgccacg
     5701 gaccccgacg cgccggtcct caccgtcacc aaggtcacct cgggcggcgg tggcggcggc
     5761 ggcggcggtg gcggcgagcc gcgcgcctgc ggcctcaccg accagggcct cgacaacacg
     5821 gcgccgttcg gcgtcgaggt ctacatccgg ggcagcgtcg ccgccgactg ggcggccgcg
     5881 ccggagaaca agctgttcca gttcggcggc aacctgctca agcgcgagct gcaggtgcag
     5941 gccggtgccg cgcagttcaa ggtggccgat gccggctggg cgtcgatcaa ctgcggcgcg
     6001 ggcgacagcg acacgttgac gctcggcgcg gccttcacga tggcctgcgg cacgaacccg
     6061 ccgaacgtca acgtcgagtt ccccgcggcc ggctgctacg agttcgcggt caa
//
LOCUS       DASPFR010000239         7273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1045769, whole genome shotgun sequence.
ACCESSION   DASPFR010000239 DASPFR010000000
VERSION     DASPFR010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7273
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045769"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VD737_06205"
     CDS             <1..443
                     /locus_tag="VD737_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10186.1"
                     /translation="SPDAPLEHRWETGLATGDFGGANVVSVTGSWVANPHLLLRTDVS
                     QLLGTYSDGWLGTIGIAHVFVPQWRLSPFAGLGGGLLTISPKATLVQAEDRSDSTAYA
                     GLGVRGFLTQRFMLQAEYRNYVVFTSRDENEEIDAWTVAFTYYF"
     gene            413..1078
                     /locus_tag="VD737_06210"
     CDS             413..1078
                     /locus_tag="VD737_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HXW10187.1"
                     /translation="MDRGVHVLLLTVLATVAWPSVAPAQVSAPAAALPTQQPQVVEPR
                     IERRDVSAPDIDTENFEGGLVIGTVSIEDFGSSLLYGARLAYHFSEDLFAELNLGSSK
                     AGRTSYEDLSGSAQLLTDSERQYTYYDLGVGWNALPGELFFGGTRAMPSAVYLTLAAG
                     NTRFAGDDHFSVALGAGLRVLATDWLAVHLDVRDRMFESDLLGENKNTQNIQVGLGLT
                     AFF"
     gene            1090..1590
                     /locus_tag="VD737_06215"
     CDS             1090..1590
                     /locus_tag="VD737_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXW10188.1"
                     /translation="MRSTWSSLLLACMALLTPAAHAGGLAAGAPAPDFVLASRDGSKV
                     RLADLRGDVVMINFWASWCGPCRQEMPLLAQLQAKYEPLGFRLLGINVEPDSREALAW
                     LEGVPVTFPILFDTASEVAGRFGVQGMPSTVFVDRAGNVRYVHQGYKPGDESRYADMI
                     RSLVKE"
     gene            1593..1823
                     /locus_tag="VD737_06220"
     CDS             1593..1823
                     /locus_tag="VD737_06220"
                     /inference="COORDINATES: protein motif:HMM:NF025452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HXW10189.1"
                     /translation="MRNVPRALLVLSLGLASAGCSDLQPWVKPYERQNFADILMAAQR
                     DPIAAAYLDHVFEAREGARGATGSHGGGCGCN"
     gene            1833..2978
                     /locus_tag="VD737_06225"
     CDS             1833..2978
                     /locus_tag="VD737_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HXW10190.1"
                     /translation="MSNGSRKTALSTLAGSLVASTTAHGAVLPEDRADVMYHRYDGGG
                     VTIDGPSLLMRKKFGEKYAFSASYYMDMVSSASIDVVTTASPYRETRTQGSLGVDVLQ
                     GKTQYSLSFTSSDENDYTANTASFDLSQDVFGDLTTVSFGFSQGSDTVGKRGDGAFEE
                     DVDRRNYRLGLSQILTPSLMAGLNFEVITDEGFLNNPYRSVRYVDESNGRGYSYQPEQ
                     YPHTRNSNAVALNARYFLPSRAAVHGEYRYYTDTWGIDAHTASIGYTRSLGKPWLLEV
                     GYRWYDQSNADFYSDLFPYADAQNFLARDKELSTFTSHMLSLGATYELPPRGWEWLKK
                     STVNVFVDQIRYDYADFRDLTRSGAPGSEPLYQYDATVLRVFISAWF"
     gene            3118..3510
                     /locus_tag="VD737_06230"
     CDS             3118..3510
                     /locus_tag="VD737_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXW10191.1"
                     /translation="MTRIEHVALWARDLELLCLFYARAFDATVGPLYENQAKGFKSRF
                     LSFESGARIEVMATSQLQPVQHAPGAQRMGLTHLAFSLGSEAAVDRLTEKLTREGAVL
                     LDGPRLTGDGYYESVVLDPEGNRLELTV"
     gene            complement(3547..4245)
                     /locus_tag="VD737_06235"
     CDS             complement(3547..4245)
                     /locus_tag="VD737_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10192.1"
                     /translation="MRTALTLLPVVGLALAGCASIQPARMVLPEGLAAASQEAVLRGL
                     GGGREGEFALAGNHGRFQRTNDRLDVFDIVSFDWGGSRLTIEGVDVETPVSASCGLRQ
                     VTAGWRIVRFAAKPIAYECEFTGIDAGLSLQEAKSTRDALASKARRRGEARVQGLVLE
                     LRSVHELEGTPLTVEAPIGYVVEQSGRAIGAIELNGSTPRVWLPPDDATQRLAVLLAV
                     MPLALLWDPAALHD"
     gene            complement(4343..>7273)
                     /locus_tag="VD737_06240"
     CDS             complement(4343..>7273)
                     /locus_tag="VD737_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10193.1"
                     /translation="YGESSLGLRNIPLASGSVTVRGSAIPPGHTVWVAGRPVPVDANG
                     SFVAEEILPDGAHTVEVAVLDDAGNGSLYLRDMEFKRNDWFYVGMADLTLAETRSNGP
                     AEALQGKDAASDFDSPANGRLAFFVNGKWRDDWKITASADTREAPIEDLFSNFLDKSP
                     ESLFRRIDPDNHYPTFGDDGVVEEMAPTLGKFFVRASKGQNYGQWGNFKVGYVGNELA
                     HVDRGLYGANAHYASEETTGFGERRLALDGFAAEPGTLASHEEFRGTGGSLYYLHHQD
                     VMGGSERLRIEVRDKVSGIVNGVVNLRPGMDYDIDYLQGRVLLTEPLSSTAGDNLLVR
                     SSGVSGDEAVLVVRYEYTPGFDELDAVAVGGQGHYWFNDHVKLGLTASTSDEGDTDSS
                     LNAADLTLRMTSDTWVKLQAGQSEGLVSGAMQSGDGGFEFVGQDPAAYVDADAGAYRA
                     DVNVGFGDFLAGRRERLNLYVQSLEAGYSAPGQSTLKDTEFFGGSLTLPLSPRLTVAA
                     KGDLMNQDRGLETSALELDVGYQVTDFWKLSSGIRNELRKDRSALVPLTQEEGERTDA
                     VVQATYDPGTTWSAYGFVQETLASTGDREDNGRLGAGGTYRITERFKLDAEVSAGDLG
                     PGARVGSNFLYTERTNLYLNYSLENERTDNGLQARRGNLVSGVKTRLSDSASVYMEER
                     YQDTESLTGLTHATGVNLAADERWTLSANADVGRLTDAITGAETDRKAGGIRVGYGFE
                     RVQLSSAVEYRLDDTERPDASTTERTTWLFRNTFKYQLSPDWRIVGKLNHSTSESSDG
                     NFFDGGYTEGVIGYAYRPVDNDRLNALAKYTYFYNVPTTDQVLQANTPVEFVQKSHIA
                     ALDLTYELTPDWSIGGKYAYRLGQVSLDREDPEFFDNTAHLLVMRVDWRFRGDWEMVT
                     EARMLALPDVEQQRTGALLGVYRSMGQHLKLGVGYNFTDFSDDLTDLSYDHQGAFLNV
                     VGML"
BASE COUNT         1134 a         2469 c         2441 g         1229 t
ORIGIN      
        1 cttcgcctga cgcgccgctg gagcatcgct gggagaccgg gctggcgacc ggcgattttg
       61 gtggcgcgaa cgtcgtttcg gtcactggca gctgggtggc gaatccccac ctgctgctgc
      121 gcaccgacgt gagccagttg ctcggaacgt attcggacgg ctggctcggc acgatcggca
      181 ttgcgcacgt gttcgtgccg cagtggcgcc tttcgccatt cgctgggctc ggcgggggcc
      241 tgttgacgat ctcgccgaag gccacgctcg tgcaagccga ggaccggtcc gattcgacgg
      301 cctacgccgg gctcggcgtc cgcggcttcc tgacgcagcg gttcatgctc caggccgagt
      361 accgcaacta cgtcgtcttc accagccgcg acgagaacga ggagatcgat gcatggaccg
      421 tggcgttcac gtactacttc tgaccgtgct ggcgacggtg gcctggccgt ccgtcgcgcc
      481 ggcacaggtc agcgcgccag ccgccgccct gcccacgcaa cagccgcagg tcgtcgagcc
      541 caggatcgag cggcgtgacg tctcggcgcc cgacatcgac accgagaact tcgagggcgg
      601 cctggtcatc gggaccgtga gcatcgaaga cttcggttcg agcctgctgt acggcgcacg
      661 acttgcctac catttcagcg aggacctgtt tgcggaactc aacctgggtt cgtccaaggc
      721 cggaaggacg agctacgagg acctgagcgg ctcggcgcaa ctgctcaccg acagcgagcg
      781 ccagtacacc tattacgacc tcggcgtcgg gtggaacgcg ctgcccggcg aactgttctt
      841 cggtggcacg cgcgccatgc cgagcgccgt ctacctgacg ctcgccgccg gcaacacccg
      901 cttcgcgggc gacgaccact tttcggtcgc gctcggagcc gggctgcgcg tgctggccac
      961 cgactggctc gcggtgcacc tcgacgtgcg cgaccgcatg ttcgagagcg acctgctcgg
     1021 ggagaacaag aacacgcaga acatccaggt cggcctgggc ctgacggctt tcttctgagg
     1081 atgccaacga tgcgctcgac atggtcctcg ctgttgctgg cctgcatggc gttgctcact
     1141 ccggccgcgc acgccggagg cctcgcggcc ggcgctccgg cgcccgattt cgtgctcgcc
     1201 tcgcgtgacg gcagcaaggt caggctggcg gacctgcggg gcgacgtggt gatgatcaac
     1261 ttctgggcga gctggtgcgg cccctgccgg caggaaatgc cgctgctggc gcagttgcag
     1321 gccaagtacg aaccgctcgg cttcaggctg ctcggcatca acgtagagcc ggacagccgg
     1381 gaggcgctcg cgtggctgga aggggtgccc gtcacgttcc cgatcctgtt cgacactgcg
     1441 agcgaggtcg ccggtcgatt cggcgtccaa ggcatgccca gcacggtgtt cgtcgaccgc
     1501 gcaggcaacg ttcgctacgt gcaccagggc tacaagcccg gcgacgaatc caggtacgcg
     1561 gacatgatcc gcagcctggt caaggaatga acgtgcgcaa cgtaccgcga gcgctgctcg
     1621 tgctgtcgct tggactcgcc tccgcgggat gcagcgacct gcaaccctgg gtaaagccct
     1681 atgaacggca gaatttcgcc gacatcctga tggcggccca acgtgacccg atcgcggcgg
     1741 cgtacctcga tcacgtgttc gaggcgcgcg aaggcgcgcg cggcgcgacg ggcagccacg
     1801 gcggcggctg cggctgcaac tgagggcgac cgatgtcgaa cggttcccgc aagaccgcac
     1861 tgtcgacgct cgccggatcg ctcgtggcga gcaccacggc ccacggtgcg gtgcttcccg
     1921 aggaccgggc ggacgtcatg tatcaccgct acgacggcgg cggcgtgacg atcgacggcc
     1981 cgtcgctgct gatgcgcaag aagttcggcg agaagtacgc gttctccgcc agctactaca
     2041 tggacatggt gtcgagcgcg tcgatcgacg tcgtcaccac ggcgagcccg taccgcgaga
     2101 cgcgcacgca gggcagcctc ggggtcgacg tactgcaggg caagacgcag tacagcctca
     2161 gtttcacgtc gagcgacgag aacgactaca cggcgaacac ggccagcttc gacctgagcc
     2221 aggacgtctt cggggacctc acgaccgttt cgttcggctt cagccaggga tcggacaccg
     2281 tgggcaagcg cggcgacggg gcgttcgagg aggacgtcga ccgtcgcaac tacaggctcg
     2341 gcctctcgca gatcctcacg ccgagcctga tggccggcct gaacttcgag gtgatcacgg
     2401 acgaggggtt cctgaacaac ccgtatcggt cggtgcgtta cgtggacgaa tcgaacggcc
     2461 gcggctactc gtaccagccc gagcagtacc cgcacacgcg caacagcaac gccgtcgcgc
     2521 tgaacgcgcg ctatttcctg ccgtcacggg cggccgtgca cggcgagtac cgctattaca
     2581 ccgacacgtg gggcatcgac gcgcacactg cctcgatcgg ctacacccgt tcgctgggca
     2641 agccctggct gctggaggtc ggttatcgct ggtacgacca atccaatgcc gacttctact
     2701 ccgacctgtt tccctacgcc gacgcgcaga atttcctggc tcgcgacaag gagctcagca
     2761 cgttcacgag tcacatgctg agcctcggcg ccacgtacga actgccgccc cgcggctggg
     2821 agtggctgaa gaagtcgacc gtgaacgtgt tcgtcgacca gatccgctat gactacgccg
     2881 acttccgcga cctcaccagg agcggtgcgc cgggatcgga gccgctctat cagtacgacg
     2941 cgaccgtgct gcgcgtgttc atctcggcct ggttctaggc acgccgcgct cgcctgcgtc
     3001 ctcgcagacg actcccgcaa cccgctggaa aggcgagcgc atggacttct ggctcgcgat
     3061 ccactatcca tgggacccgc ggttacgact cgcccgtcgt acgggaagac gcataccatg
     3121 acgcgcatcg aacacgtcgc actgtgggca cgggatctcg agctcctgtg cctgttctat
     3181 gcgcgagcgt tcgacgccac ggtcggtccc ctgtacgaga accaagccaa ggggttcaag
     3241 tcgaggttcc tgagtttcga atcgggtgca cgcatcgagg tcatggccac gtcacagttg
     3301 cagccggtgc agcacgcgcc gggggcgcaa cgcatgggtc tcacgcacct ggccttttca
     3361 ctggggtctg aagccgcggt ggatcgattg accgagaagt tgacgcgaga gggcgcggtg
     3421 ctgctcgatg gcccacgtct cacgggtgac ggatactacg agagtgtcgt gctcgatccc
     3481 gagggcaatc gcctcgagct gacggtctag cgtgcgcgcc atcgagttac ggggcgtggc
     3541 accccgctag tcgtgcagtg ccgccggatc ccacaacagc gccagcggca tcactgcgag
     3601 cagcaccgcg agtcgttgcg tcgcatcgtc cggcggcagc cacacgcgcg gggtcgaacc
     3661 gttgagctcg atggccccga tcgcgcgtcc cgactgctcc acgacgtaac cgatcggcgc
     3721 ctccaccgtc agcggggtgc cctcgagctc atggactgaa cgaagctcca gcaccaggcc
     3781 ctggacgcgt gcttcgccgc gacgccgcgc cttgctggca agcgcgtcgc gcgtggactt
     3841 cgcctcctgc agcgaaagcc cggcgtcgat gccggtgaac tcgcattcgt aggcgatcgg
     3901 cttcgccgca aagcgcacga tccgccagcc cgcggtgacc tggcgcaggc cgcaactggc
     3961 ggacaccgga gtctcgacgt cgacaccctc gatcgtcagg cgggagccgc cccagtcgaa
     4021 gctgacgatg tcgaacacgt ccagccgatc gttggtgcgc tggaatcggc cgtgattgcc
     4081 ggccagcgcg aactcgcctt cccgcccacc gccgaggccg cgcaggacgg cctcctgcga
     4141 cgccgccgcg aggccttcgg gcagcaccat ccgggcgggc tggatcgacg cgcagccggc
     4201 cagcgccaat ccgacgaccg gcaacagcgt cagggctgtt cgcatacctg gacttcactg
     4261 gcaggcacgc acgacgcgtg cagcgcaatg atgtcccagg tccgatcccg aatcgactcg
     4321 agctgcgagc gattcgccag gatcagagca tcccgaccac gttcaggaac gccccctggt
     4381 ggtcgtagct caggtccgtc aggtcgtccg agaagtccgt gaagttgtag ccgacgccga
     4441 gcttcaggtg ctggcccatc gaccggtaga cgcccaggag cgcgccggtg cgctgctgtt
     4501 cgacgtccgg cagggcgagc atgcgcgcct cggtcaccat ctcccagtcg ccacggaagc
     4561 gccagtcgac gcgcatcacc agcaggtggg cggtgttgtc gaagaactcc ggatcctcgc
     4621 ggtccaggct cacctgtccg agtcgatagg cgtacttgcc accgatcgac cagtcgggcg
     4681 tcagctcgta ggtcaggtcg agcgccgcaa tgtggctctt ctgcacgaac tcgacgggcg
     4741 tgttggcctg cagcacctgg tcggtcgtcg gcacgttgta gaagtacgtg tacttggcca
     4801 gcgcgttgag ccggtcgttg tcgaccgggc ggtacgcata gccgatcacg ccctcggtgt
     4861 agccgccgtc gaagaagttg ccgtccgagc tttcgctggt cgagtggttc agcttgccga
     4921 cgatgcgcca gtcgggcgac agctggtact tgaacgtgtt gcggaacagc caggtcgtgc
     4981 gttcggtggt gctcgcgtcc ggccgctcgg tgtcgtcgag ccggtactcg accgcgctcg
     5041 agagctgtac gcgctcgaac ccgtagccga cgcggatgcc gcccgccttg cggtcggtct
     5101 cggcgccggt gatggcgtcg gtgagccggc cgacgtcggc gttcgcactc agcgtccagc
     5161 gctcgtcggc cgccaggttg acgcccgtcg cgtgggtcag gcccgtgagc gactcggtgt
     5221 cctggtagcg ctcctccatg tacacgctcg cgctgtccga caggcgcgtc ttgacgcccg
     5281 agaccaggtt gccgcgtcgc gcctgcaggc cgttgtccgt gcgctcgttc tcgagcgagt
     5341 agttcaggta gaggttggtg cgctccgtgt acaggaagtt gctgccgacc cgtgcgcccg
     5401 ggccgaggtc gccggccgag acttccgcgt cgagcttgaa gcgttcggtg atgcggtacg
     5461 tgccgccggc gcccaggcgg ccgttgtcct cgcggtcgcc ggtggaggcg agcgtttcct
     5521 gcacgaagcc gtacgcgctc caggtcgtgc ccgggtcgta ggtggcctgt acgaccgcgt
     5581 cggtccgctc gccctcctcc tgcgtgagcg gcacgagcgc ggaccggtcc ttgcggagct
     5641 cgttgcggat gccgctgctg agcttccaga agtccgtgac ctggtacccg acgtcgagct
     5701 cgagcgccga cgtctcgaga ccgcgatcct ggttcatcag gtcgcctttg gccgcgacgg
     5761 tcaggcgcgg cgacaacggc agcgtcaggc tgccgccgaa gaattcggtg tccttgagcg
     5821 tcgactggcc cggtgcggaa tagccggcct cgaggctctg cacgtagagg ttgaggcgct
     5881 cgcggcggcc ggcgaggaaa tcgccgaacc cgacgttcac gtccgcccgg tacgcgcccg
     5941 cgtcggcgtc gacgtacgcc gccggatcct ggccgacgaa ctcgaagccg ccgtcgccgg
     6001 actgcatggc cccggagacg agtccttcgc tctggccggc ctgcagcttg acccacgtgt
     6061 cggaggtcat gcgcagcgtc aggtccgccg cgttgaggct gctgtcggtg tcgccctcgt
     6121 cgctggtgct ggccgtcagc ccgagcttca cgtggtcgtt gaaccagtaa tggccctgcc
     6181 cgcccaccgc gaccgcgtcg agctcgtcga agcccggcgt gtactcgtag cgtacgacca
     6241 ggactgcctc gtcgccgctc acgccgctcg agcgcacgag caggttgtcg cccgccgtgg
     6301 acgacagcgg ctcggtgagc agcacgcgac cctgcaggta gtcgatgtcg tagtccatgc
     6361 cgggccgcag gttgaccacg ccgttgacga tgcccgagac cttgtcgcgc acctcgatgc
     6421 ggaggcgctc cgagccgccc atcacgtcct ggtggtgcag gtagtacagc gacccgccgg
     6481 tgccccggaa ctcctcgtgg ctcgcgagcg tgccgggctc ggccgcaaac ccgtcgaggg
     6541 ccagccggcg ctcgccgaag ccggtcgtct cctcggaggc gtagtgcgcg ttggcgccgt
     6601 acagcccgcg gtcgacgtgc gccagctcgt tgccgacgta gccgaccttg aagttgcccc
     6661 actggccgta gttctgtccc ttgctcgccc tgacgaagaa cttgccgagc gtgggcgcca
     6721 tctcctcgac cacgccgtcg tcgccgaagg tcgggtagtg attgtcgggg tcgatgcgcc
     6781 tgaacagcga ctcgggcgac ttgtcgagga agttgctgaa caggtcctcg atcggcgcct
     6841 cgcgcgtgtc ggcgctggcc gtgatcttcc agtcgtcgcg ccacttgccg ttcacgaaga
     6901 acgccagccg gccgttcgcc ggcgagtcga agtcgctcgc tgcgtccttg ccctgcaggg
     6961 cttcggccgg cccgttgctg cgggtctcgg cgagcgtgag gtcggccatg ccgacgtaga
     7021 accagtcgtt gcgcttgaat tccatgtcgc gcaggtacag cgacccgttg cccgcgtcgt
     7081 ccagaacggc cacttcgacc gtgtgggcgc cgtcgggcag gatttcctcg gccacgaagc
     7141 tgccgttcgc gtcgaccggc accggccggc ccgcgaccca gacggtgtgg cccggcggaa
     7201 tcgcgctgcc gcgcacggtc accgagccgc tcgcgagcgg aatgttgcgc aagcccaggc
     7261 tgctttcgcc gta
//
LOCUS       DASPFR010000240         5490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_191523, whole genome shotgun sequence.
ACCESSION   DASPFR010000240 DASPFR010000000
VERSION     DASPFR010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5490
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191523"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VD737_06245"
     CDS             complement(<1..485)
                     /locus_tag="VD737_06245"
                     /inference="COORDINATES: protein motif:HMM:NF017833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HXW10194.1"
                     /translation="MKVHLQPVRRDPNARRRLSEMLDSIANDPARETIAAVDVRDAMG
                     DRAFGALMFFFAAPNALPVNMPGVSAVLGLPLLFLSLQLMLGFAVPWLPRFLLHRTFP
                     RKGFARVMSHALPWIRRAERLLRPRLTALADGAFERVIGLACVVLAAVLILPIPFANT
                     GP"
     gene            complement(482..1474)
                     /locus_tag="VD737_06250"
     CDS             complement(482..1474)
                     /locus_tag="VD737_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HXW10195.1"
                     /translation="MLAMELTAPRRPLVAVDRAIPMPGSGQLRVRVTACAVCRTDLHV
                     VDGELPDVRHPIVPGHEIVGVVEARGPGTTTTLGTRVGIPWLAHTCGRCDHCTHGREN
                     LCDEARFTGCHVDGGYAEYAVADERYCLALPAELEDVHAAPLLCAGLIGYRALVMAGE
                     ARRLGLYGFGAAAHIVAQVALHQGREVHAFVRPGDEPAKAFARRLGCAWAGDATSGPA
                     APLDAAIIFAPAGELVPQALGAVRKGGTVVCGGIHMSDIPSFPYARLWGERVLRSVAN
                     LTRRDGDEFLPLAARIPVRTEVTTWPLEAANDALDALRSGRLTGAAVLIPPRAA"
     gene            1892..3379
                     /locus_tag="VD737_06255"
     CDS             1892..3379
                     /locus_tag="VD737_06255"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HXW10196.1"
                     /translation="MSGPARAQAQGGDAAAAKLAATQALAAEMPFNPGKPQEHGAAAR
                     TPPAGTVVEVPEPSATSSTLTEGSASPKVGEGRPALGLNPTNGPLDRVRVDSSGRVLT
                     TNQGVPVGDNQNSLKAGLRGPALLEDFILREKITHFDHERIPERIVHARGSGAHGYFE
                     CHRPLTDLTRASIFAEAGKRTPVFVRFSTVAGERGSTDTARDVRGFAVKFYTDEGNWD
                     LVGNNIPVFFIQDAMKFPDLVHAVKPEPHHGMPQAASAHDTFWDFISLMPESAHMIMW
                     VMSDRAIPRSYRMMQGFGVHTFRFVDADGGSRFVKFHWNPVLGTHSLDWDEAVKISGA
                     DSDFHRRDLWEAIEAGQFPEYELGVQVFTEEQADGFTFDVLDATKIVPEELVPVTPVG
                     RLVLNRNPDNFFAETEQVAFCTAHVVPGVDFTNDPLLAGRIHSYVDTQISRLGGRTST
                     RSPSTRRWRRPTTTSATACTARRSTAVASHTSRTRSGAAVRSRRA"
     gene            3376..4224
                     /locus_tag="VD737_06260"
     CDS             3376..4224
                     /locus_tag="VD737_06260"
                     /inference="COORDINATES: protein motif:HMM:NF036542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-related domain-containing protein"
                     /protein_id="HXW10197.1"
                     /translation="MSGFTSFPRFAEPDKVRGKPEKFADHYTQATLFYRSQAPVEQAH
                     IQRALRFELTKVQVPAVRERVVAMLRNIDEKLAARVAGDLGMELPDALPRAIARVPKP
                     EVAESPALSLFARPGDGSIRTRRVAILVATGSDLGHAVELHERLTELGAIPRYVANVL
                     GTVVGAKGDSVESEVTFETMPSVLVDAVAVPCGERAAKALANVGQAVESIVNAYRHCK
                     PILAWGAGRSLVENCGVPATLPAGDPDPGLLLVETADFDDGVEGFVAAIARHRHFERQ
                     VDPPAV"
     gene            4260..4889
                     /locus_tag="VD737_06265"
     CDS             4260..4889
                     /locus_tag="VD737_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HXW10198.1"
                     /translation="MPTTPSQMQELLYQALETEQGGIKVYEAAIECAVNDDLREEWTE
                     YLEQTREHERTLRETLQAVGLQPDTETPGRRVVRHIGESLVAAIEMARQGGGPPEAAE
                     LVAAECVVFAETKDHMNWELIGRLGLDLDGKEAEVLKKAYDRVEDQEDAHLYHSKGWT
                     RELWIHALGLPAVLPPPEEVQKVETAIGAARAEASREVLLGNTPQTRRR"
     gene            complement(4939..>5490)
                     /locus_tag="VD737_06270"
     CDS             complement(4939..>5490)
                     /locus_tag="VD737_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXW10199.1"
                     /translation="DVVVNNAGITHRSAFEATQTTVLRRVMDVNLFGAIHLTRAALPH
                     LKRTRGLVIALSSVAGYTPLIARTGYAASKHAMHGFFESLRAELTPSGVGVMLVCPSF
                     IATGIDRNALGPTGGPATHPQVVVGGRLQPDDVADRIVRGAERNRRRLLIGRTAHAAW
                     WVSRVAPGLYARIMARKLREELG"
BASE COUNT          910 a         1911 c         1911 g          758 t
ORIGIN      
        1 ggccccgtgt tcgcgaacgg gatgggcagg atcagcaccg cggccagtac gacgcaggcg
       61 agcccgatca cgcgctcgaa cgcgccatcg gcgagcgcgg tcagccgtgg tcgcagcagg
      121 cgctcggcgc gccggatcca cggcagcgca tggctcatga cgcgcgcaaa acccttgcgc
      181 gggaatgtcc tgtgcagcag gaatcgcggc agccagggca cggcgaaccc gagcatcagc
      241 tgcagggaca ggaacagcag cggcaggccc agcacggcgg aaacaccggg catgttgacc
      301 ggcagcgcat tgggcgccgc gaagaagaac atcagggcgc cgaacgcacg gtcccccatc
      361 gcgtcgcgca cgtcgacggc ggcgatcgtc tcgcgcgccg ggtcgttggc gatcgaatcc
      421 agcatctccg agagtcgtcg acgcgcgttc gggtcgcgtc gtaccggctg caggtgaact
      481 ttcatgcggc gcgcggtggg atcagcacgg cggcacccgt cagccggccg gagcgcaagg
      541 catcgagcgc atcgttggcg gcctcgagcg gccacgtggt cacctcggtg cggaccggga
      601 tcctcgcggc cagcggcagg aactcatcgc cgtcgcggcg cgtgaggttg gcgaccgaac
      661 gcagcacgcg ttcgccccag agccttgcgt acgggaacga cgggatgtcg ctcatgtgga
      721 tgccgccgca gacgaccgtg ccgcccttgc gcacggcccc gagcgcctgc ggcacgagct
      781 cgccggccgg cgcgaagatg atcgcggcgt cgagcggcgc cgccggcccg ctggtggcgt
      841 cgccggccca cgcacagccg aggcgtcgtg caaacgcctt ggccggctcg tccccgggac
      901 ggacgaacgc gtggacttcg cgaccctggt ggagcgcgac ctgcgccacg atgtgcgcgg
      961 cggcaccgaa gccgtagagg cccagccggc gagcctcacc ggccatcacc agcgcgcgat
     1021 agccgatcag ccccgcgcag agcagcggcg ccgcgtggac gtcctccagc tccgcgggca
     1081 gcgcgagaca gtagcgctcg tccgcgaccg cgtactcggc gtagccgccg tcgacgtgac
     1141 agcccgtgaa gcgcgcttcg tcgcagaggt tttcgcgacc gtgcgtgcag tgatcgcacc
     1201 gaccgcaggt gtgggcgagc cacgggatgc cgacgcgcgt gccgagcgtc gtcgtcgtcc
     1261 cgggcccgcg cgcctcgacg acgcccacga tctcgtgacc cggcacgatc ggatgccgca
     1321 cgtccggcag ctcgccgtcg acgacgtgca ggtcggtgcg gcagacggcg cacgcggtga
     1381 cgcgtacgcg cagctgcccg ctgccgggca tcgggattgc gcggtcgacc gcgaccagcg
     1441 gtcgacgtgg cgccgtgagt tccatcgcga gcatgcccgg atcataacgc gtcggtgtgg
     1501 aacgtcttcc tgtcggccag ccttcgcgcg gccggcgtaa gttgcggccc tacgctcgat
     1561 tgagcttccg accgattcgt gatgcgcgcg ccgcacagga tcctcgtcac ccctccctgc
     1621 cgttcccgtc ccctctctgc cgttgtcatc gcaatgagcg ccgtcggcgg cacgacgacc
     1681 gccggcgtgc tcggagattg cgcagacagg tccacccgtc acgagctcgc aaaggctgaa
     1741 acacccgatg gcgaagaaac gagcacctac ccgcaccgga cgcagtcccg tgaagtcgtc
     1801 gccgacgcgg gccaagacat ccgcgaggcg accgaccgtc gatcgtccag gcacgcgatc
     1861 tggcgcgacc gcgacgagcg cacgctcgac cgtgagcggc ccggcacgtg cgcaggccca
     1921 gggcggcgat gccgccgccg caaaactcgc cgcgacacag gcgctcgcag cggagatgcc
     1981 gttcaacccc ggcaagccgc aggagcacgg cgctgcggcg cgcacgccgc cggccggcac
     2041 cgtcgtcgag gtgcccgagc cctccgccac gtccagcacc ctgaccgagg gttcggcctc
     2101 gccgaaagtg ggcgagggca ggccggcgct cggcctcaat ccgacgaacg gaccgctcga
     2161 ccgcgtgcgt gtcgattcga gcggacgggt gctgacgacc aaccagggcg tgccggtcgg
     2221 cgacaaccag aactcgttga aggccggatt gcgtggcccg gcgctgctcg aggatttcat
     2281 cctccgcgag aagatcacgc atttcgacca cgagcgcatt cccgagcgca tcgtgcacgc
     2341 gcggggctcg ggggcccacg gctacttcga gtgtcacagg ccgctgaccg acctgacgcg
     2401 ggcatcgatc tttgccgaag ccggcaagcg cacgccggtg ttcgtgcgct tctcgacggt
     2461 cgccggcgag cgcggctcga cggataccgc gcgcgacgtg cgcggcttcg cagtcaagtt
     2521 ctacaccgac gaaggcaact gggacctcgt cggcaacaac atcccggtgt tcttcatcca
     2581 ggacgcaatg aagtttcccg acctcgtgca cgcggtgaag cccgagcccc accacggcat
     2641 gccgcaggcg gcttccgcgc acgacacgtt ctgggacttc atctcactga tgcccgagtc
     2701 cgcgcacatg atcatgtggg tgatgtcgga ccgcgcgatc ccccgcagct accgcatgat
     2761 gcagggcttc ggcgtccaca cgttccggtt cgtcgacgcg gatggcgggt cgcggttcgt
     2821 gaagttccac tggaacccgg tgctcggcac gcattcgctc gactgggacg aagcggtcaa
     2881 gatctcgggc gcggactccg acttccatcg tcgcgatctc tgggaagcga tcgaggcggg
     2941 gcagttcccc gaatacgaac tcggcgtgca ggtcttcacc gaggagcagg cggacgggtt
     3001 cactttcgac gtgctcgacg cgacgaagat cgtgcccgag gaactggtgc ccgtgacccc
     3061 ggtgggtcgc ctggtgctga accgcaatcc cgacaacttc ttcgccgaga ccgagcaggt
     3121 ggcgttctgc acggcgcacg tcgtgccggg cgtcgacttc acgaacgatc cgttgctggc
     3181 cggccgcatc cactcgtacg tcgacaccca gatctcgcgg ctcggcggcc gaacttccac
     3241 gagatccccg tcaacgcgcc ggtggcgcag gcccacaaca accagcgcga cggcatgcac
     3301 cgccaggcga tcaaccgcgg tcgcgtcgca tacgagccga actcgctcgg gggcggctgt
     3361 ccgttccagg cgggcatgag cggattcacg tcgttcccca ggttcgcgga gcccgacaag
     3421 gtgcgcggca agccggagaa gttcgcggac cactacacgc aggcgacgct gttctatcgc
     3481 agccaggcgc cggtcgagca ggcgcacatc cagcgcgcgc tgcgcttcga gttgaccaag
     3541 gtccaggtgc cggccgtacg cgagcgcgtc gtcgcgatgc tgcgcaacat cgacgaaaaa
     3601 ctcgccgccc gggtggccgg ggacctgggg atggagttgc cggatgcgct gccgcgtgcg
     3661 atcgcgcgag tgccgaaacc cgaggtcgcc gagtcgcccg cgctctcgct gttcgcacgc
     3721 ccgggcgacg gcagcatccg gacccgacgc gtcgcgatcc tggtggcgac cggcagcgac
     3781 ctcggtcacg ccgtcgaact ccacgagcgc ctgaccgaac tcggggcgat cccgcgctac
     3841 gtcgcgaacg tgctcggcac ggtcgtcggc gcgaaaggcg actcggtgga gtccgaagtc
     3901 acgttcgaga cgatgccctc ggtgctggtg gacgcggtcg cggtgccctg cggcgagcgg
     3961 gcggccaagg cgcttgccaa cgtcggacag gccgtcgagt ccatcgtcaa cgcgtatcga
     4021 cactgcaagc cgatcctggc ctggggcgcc ggacgcagcc tggtcgagaa ctgcggggtg
     4081 ccggcgaccc tgcctgccgg cgacccggat cccgggctgc tgctggtcga gaccgccgat
     4141 ttcgacgacg gcgtggaagg tttcgtggca gcaatcgccc ggcaccggca cttcgagcga
     4201 caggtcgatc cgccggcggt ctagtcgctt ccccacagga cttcacgaga gcaacaccga
     4261 tgccgacgac acccagccag atgcaggaac ttctctacca ggctctcgag acggagcagg
     4321 gtggcatcaa ggtctacgaa gccgcgatcg aatgcgcggt caacgacgat ctacgcgagg
     4381 agtggaccga gtacctcgag cagacgcgcg agcacgagcg cacgctgcga gaaacgttgc
     4441 aggccgtggg actccagccc gacaccgaga cgccgggacg ccgggtcgtt cgtcacatcg
     4501 gggagtcgct ggtggccgcc atcgagatgg cacgccaggg cggcgggcct ccggaagcgg
     4561 cggaactcgt ggcggccgag tgcgtcgtct tcgccgagac caaggaccac atgaactggg
     4621 aactgatcgg ccggctggga ctcgacctgg acggcaagga agccgaggtt ctcaagaagg
     4681 cgtacgaccg ggtcgaggac caggaggacg cgcacctcta ccacagcaaa ggctggaccc
     4741 gcgaactctg gatccacgcg ctcgggctgc ctgcggtgct gccaccgccc gaggaagtgc
     4801 agaaagtcga gacggcgatc ggcgcggcaa gagccgaggc gagccgggag gtcctgctcg
     4861 gcaacacgcc gcagacgcga cggcgctgag tgcaagctcg cgcgacggga ctgcgcgttt
     4921 tcctcggcgt cggcgagttc agcccaactc ctcgcgcagc ttccgcgcca tgatgcgggc
     4981 gtagagtccc ggtgcgacgc ggctgaccca ccacgccgcg tgcgcagtcc ggccgatcag
     5041 cagtctccgc cggttgcgct cggcgccgcg cacgatccga tcggccacgt cgtcgggctg
     5101 tagccggccg ccgaccacga cttgtgggtg cgtcgccggc ccgccggtcg ggccgagcgc
     5161 gttccggtcg atgcccgtgg cgatgaacga cgggcacacg agcatcacgc cgacgccgga
     5221 cggcgtgagt tcggcgcgca ggctctcgaa gaagccgtgc atcgcgtgct tgctcgcggc
     5281 gtagcccgtg cgcgcgatca gcggcgtgta gccggccacg ctcgacagtg cgatcaccag
     5341 gccacgcgtg cgtttcagat gcggcagcgc ggcgcgggtc aggtgaatcg cgccgaacag
     5401 gttcacgtcc atcacccggc gcagcaccgt ggtctgcgtc gcctcgaacg cgctgcgatg
     5461 cgtgatgccg gcgttgttga cgacgacgtc
//
LOCUS       DASPFR010000241         9451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_191528, whole genome shotgun sequence.
ACCESSION   DASPFR010000241 DASPFR010000000
VERSION     DASPFR010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9451
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191528"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VD737_06275"
     CDS             complement(<1..743)
                     /locus_tag="VD737_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXW10200.1"
                     /translation="MSAHDTPASRAGGSHVLLHVFLIAFTLLAIYPVLWVVSVAFSGT
                     QNLAFADVPPDPGFVDRLRAVLPWPAQWSFANFTDVLTEQPFLRWIINSAIVALSTTV
                     VGVFLACTAAYAFSRFRFPGRRAGMMSFLVSQMFPGTLLLVPLYIIIVSWLKLGNSFI
                     GLVLVYSVTAIPFCVWMLKGYFDTIPLEIEESAIMDGASRQTIFFRIILPLAKPAVAV
                     TALFSFMTGWNEFILAATFLEEETKYTAPV"
     gene            complement(740..2776)
                     /locus_tag="VD737_06280"
     CDS             complement(740..2776)
                     /locus_tag="VD737_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HXW10201.1"
                     /translation="MSEIAGNVRTRRQAAADTPSRRARRFLLQPAALGLLLALLVTVL
                     LGRWFVGQSRDELAAQTQGRMAILEASALAEAVARIVAANPDDTAPLQAALADWVARS
                     PGDDHVLVLRLSGARLLASTRATDLQGELPRRLSREEKWLFDLGQELRAAIDTNVSEG
                     VFRKAQIDVARPAEGRIRVTLPWLQDGAVAGIVQHERPLPGAATGPAPMPWPLLIVLP
                     VAAFWLVALLLQRGVAPTTRSQRWIAAAVALAMFVAAWWYFSNRATDALEATVAASNQ
                     AVAEEYRDLRARVPGAEASAEATGGAPNPWDVDVFQRPRKLLLADGSVDPVAGAQVVE
                     RMRAPLGRALGGNLLLGVAILAFFALGAARKLRETVSEYRYAYLYVTPAMVGMLILVF
                     FPFAYGITLSFTGQTIFNTNQPLTDLFVGVQNYADILGDFDVARQTAAGWSINYQNFY
                     WTLFITICWTVSNVALGVTLGMILALALNTPGLKGKAIYRVLLILPWAIPNYITALIW
                     KALFHQQFGAVNQAIQMFGGEPVAWFDGVFSSFMTGLATNGWLSFPFMMVVILGGLQS
                     ISQDMYEAATVEGATRAQQFWRITLPLLKPTLVPAVILSVVWTFNMFNVIYLVSGGEP
                     AGANEILITKAYKIAFEEYRYAYSAAYSTVIFLILLVYGVFQTRMTRATEAVNA"
     gene            complement(2790..4034)
                     /locus_tag="VD737_06285"
     CDS             complement(2790..4034)
                     /locus_tag="VD737_06285"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXW10202.1"
                     /translation="MTTTIPRRHFLRRGLGAALAVAAFGFGAAARPATDLTVWHAYRG
                     DEKAAFEKVVAQFNAKQKDIAVKTLAVPYDAYADKITASVPRGKGPDVFIFAQDRLGG
                     WVEAGQTIEPIDFYLEDATVKSLVPGMMEAMTYRDTVYGLPLNYKSIAMIYNTALVKS
                     PPKTTADLVKLAKGMTDVSTGRFGLAYEYSNYFFHAALMNAYGGQVFAPGPTPTIDSP
                     QNVAAVKTMLKWYKQDKILPADPSSTLIASLFNEGKTPIVFSGPWFLGEVQDKVKYAI
                     APLPLVSEAGGKPMKPWLTVEGVYVSAGSQHKEQAYKFAEYLISPEAAVVLALEGGQL
                     PTNRAVYTDPRVVKNPTLQGFRKQLDTAVPMPNYAEMTLMWSPVTTAMNKIVKGSATP
                     EAALKEAQETVKGNIAKLRKGR"
     gene            complement(4066..5178)
                     /gene="ugpC"
                     /locus_tag="VD737_06290"
     CDS             complement(4066..5178)
                     /gene="ugpC"
                     /locus_tag="VD737_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HXW10203.1"
                     /translation="MARIAFQGIAKRFGDVSVIENLDLEIHDHEFMVFVGPSGCGKST
                     ALRMIAGLEEPSEGQLAIGDRVVNDVHPKDRDVAMVFQSYALYPHMTVRENIAFGLKI
                     RKVPQADVDKLVDEAAGILGLKPLLDRKPKALSGGQRQRVALGRAIVRKPSVFLFDEP
                     LSNLDAELRVQMRAEIAKLQQRLKTTTVYVTHDQVEAMTLGHRIAVLAPMGKDRKSNL
                     KQVGTPLELYDRPQNVFVAKFIGTPNMNMVRATVDGDGAQLRAGGFATPVPAALRDAL
                     RAHAGREVLLGIRPEHVGEAGAHDWALTAPLGGPVEIIETIGHEVIVHFRCGDDVIIA
                     KLGAHRIPAFGEQIDLVLNADSMHIFDPATEQRIAG"
     gene            complement(5201..6664)
                     /locus_tag="VD737_06295"
     CDS             complement(5201..6664)
                     /locus_tag="VD737_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HXW10204.1"
                     /translation="MAATDPVKVTPQRNWSDAVLYFVILDRYADGAAANDTNVDRANP
                     GGFHGGDFAGLAQQLDEIASLGATAIWITPVVRQIDFCPPAQPPTGVSVPGGWFEHCA
                     FHGYWADDYTQLDPRFGTEAELRQLVEAAHARGIKVLLDVVYNHVGYDAQYVSNPATA
                     GWIRTKPVDCAVDELQCQVGGLPDLRTELPEVQRHLFDAHLGLAQRTGLDGFRLDTVK
                     HIDHAFWQKHRAEARGRLGRDFFLLAEVWGGSAQVLDDYFAGDEMDAGFDFTFKGSCE
                     AFVQGKGRTVAFAAYLEKRHNVRPGFHLAHYLSSHDEPMALHNLGGDLDAFRLCVALQ
                     LTSLGIPTIYYGEEVARQGSVWPTNRNDMPWGERPVQPGHGVARDESLREYYRKLIQA
                     RRDNPALAEGGFRRLSAEGDLLVFERVDAASGNAVVVAVNRGRAEATATVPAPSAWQG
                     SAIAEVVTGETAGLSGGQLAVSVPARQARVYVRRTTG"
     gene            complement(6956..7108)
                     /locus_tag="VD737_06300"
     CDS             complement(6956..7108)
                     /locus_tag="VD737_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008984845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HXW10205.1"
                     /translation="MGEFLRDLWQFMRVRKKYWLLPIILVLVALGGLVVVSQQSALAS
                     LIYTLF"
     gene            complement(7108..7524)
                     /locus_tag="VD737_06305"
     CDS             complement(7108..7524)
                     /locus_tag="VD737_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HXW10206.1"
                     /translation="MHRGPRALTRHDRRELRFFALGLALVIVVVFWWLMPWLRDRTAP
                     LWPLIVAWVLVVTAWMYPPAILPIHRAFLPVARVLGWLNTWLLLGTVFFAILLPVGWV
                     LRRLGKLQYVTGFDAAVDTYRVPVPPEHRLRLEDPF"
     gene            complement(7626..>9451)
                     /locus_tag="VD737_06310"
     CDS             complement(7626..>9451)
                     /locus_tag="VD737_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HXW10207.1"
                     /translation="GLSAYYHDAAAALVVDGRIVAAAQEERFTRRRHDAGFPAHAARF
                     CLAHAGLRLRDLDAVVFYDKPFLKFERLLETYLASAPHGLRSFVASMPVWLKEKLFLK
                     SELRRRLDELCGPWGTPPPLLFSEHHRSHAASAFYPSPFESAAVLCLDGVGEWATTSA
                     WFGQGTSLQPLWEQHFPHSLGLLYSAFTYYTGFKVNSGEYKMMGLAPYGTPRYAGAIR
                     EHLVDVKPDGTFRLDMRYFDYATGLRMTSERFHALFGAPPRDPEQPIVQHTMDVAASI
                     QQVTDEIVLRLAKTLRVETGARSLCLAGGVALNCVTNGRLVRERVFDDVWIQPAAGDA
                     GGALGAALVAWYAKNADAARLPAHPDAMSGALLGPETGDEALEAFLRDQDLPHETLPD
                     DAALGERVAALLADGAVVGWMQGRMEFGPRALGARSILADPRRPDMQRALNLKIKFRE
                     SFRPFAPAVLEERAAEVFELDRPSPYMLIVAPLADRPPEGAAASGDGFERLRQVQSAL
                     PTITHVDYSARVQTVDAGTNPRFRRLIEAFEQKTGVPVLVNTSFNVRGEPIVCTPADA
                     YRCFMATAMDYLVLGRHVLDRRLQPASHGLTEHAVREFEAD"
BASE COUNT         1527 a         3177 c         3315 g         1432 t
ORIGIN      
        1 accggggccg tgtacttcgt ctcctcctcg aggaacgtcg ccgccaggat gaactcgttc
       61 catccggtca tgaacgagaa gagcgccgtg acggcgaccg cgggcttggc gagcggcagg
      121 atgatccgga agaagatcgt ctgccgcgag gcgccgtcca tgatcgcgga ttcctcgatc
      181 tcgagcggga tcgtgtcgaa gtatcccttc agcatccaca cgcagaaggg tatcgcggtc
      241 accgagtaga cgagcacgag cccgatgaag gagtttccga gcttgagcca gctcacgatg
      301 atgatgtaga gcggcacgag cagcagcgtg cccgggaaca tctgcgagac caggaacgac
      361 atcatgcccg cgcgtcggcc cgggaagcgg aaccgcgaga acgcgtaggc cgccgtgcag
      421 gccaggaaca cgccgaccac cgtggtcgac aacgcgacga tggcgctgtt gatgatccag
      481 cgcaggaacg gctgttccgt cagcacgtcg gtgaagttcg cgaacgacca ctgcgccggc
      541 cagggcagga cggcccgcag gcggtcgacg aatccggggt ccggcggcac gtcggcgaag
      601 gcgaggttct gcgtgcccga gaacgccacg ctcaccaccc agagcaccgg gtagatcgcg
      661 agcagcgtga aggcgatcag gaacacgtgc agcagcacgt gcgagccgcc ggcgcgggaa
      721 gccggggtgt cgtgcgcgct cacgcgttca ccgcctcggt cgcccgggtc atgcgggtct
      781 ggaacactcc atagaccagc aggatcagga aaatcacggt cgagtaggcg gccgagtagg
      841 catagcgata ctcctcgaac gcgatcttgt aggccttggt gatcaggatc tcgttggcac
      901 ccgccggctc gccgcccgag accaggtaga tcacgttgaa catgttgaag gtccacacga
      961 ccgacaggat cacggccggc accagcgtgg gcttgagcag cggcagcgtg atgcgccaga
     1021 actgctgggc gcgcgtcgcg ccctcgaccg tcgcggcctc gtacatgtcc tgcgagatcg
     1081 actgcagccc gcccaggatc acgaccatca tgaacgggaa gctcagccag ccgttggtcg
     1141 cgaggccggt catgaacgac gaaaagacgc cgtcgaacca cgcgaccggc tcgccgccga
     1201 acatctggat cgcctggttg accgcgccga actgctggtg gaacaacgcc ttccagatca
     1261 gggcagtgat gtagttcggg atcgcccacg gcaggatcag cagcacccgg tagatcgcct
     1321 tgcccttgag accgggggtg ttgagcgcga gggccaggat catcccgagc gtcacgccga
     1381 gcgcgacgtt ggacacggtc cagcagatcg tgatgaacag cgtccagtag aagttctggt
     1441 agttgatgct ccagccggca gcggtctgcc gcgcgacgtc gaagtcgccc aggatgtcgg
     1501 cgtagttctg cacgccgacg aacagatcgg tcagcggctg gttggtgttg aagatggtct
     1561 ggcccgtgaa cgacaacgtg atgccgtacg caaacgggaa gaacaccagg atcagcatgc
     1621 ccaccatggc gggcgtcacg tagaggtagg cgtagcggta ctcgctgacc gtctcgcgca
     1681 gcttgcgcgc ggctccgagc gcaaagaacg caaggatcgc gacgccgagc agcaggttgc
     1741 cgccgagcgc ccgcccgagc ggcgcgcgca tgcgctcgac cacctgcgcg cccgcgacgg
     1801 ggtccaccga gccgtcggcc agcagcagct tgcgcgggcg ctggaagacg tccacgtccc
     1861 atggattcgg cgcaccgccg gtcgcctcgg cggaggcttc cgcaccgggc acgcgcgcac
     1921 gcaggtcgcg gtattcctcg gcgacggcct ggttgctggc ggcgaccgtg gcctcgaggg
     1981 catcggtcgc acggttcgag aagtaccacc acgccgcgac gaacatcgcc agcgccacgg
     2041 ccgccgcgat ccagcgctgc gagcgcgtgg tcggcgcgac gccccgctgc agcagcagcg
     2101 cgaccagcca gaacgccgcg accggcagca cgatgagcag cggccagggc atcggcgcgg
     2161 gcccggtggc ggcccccggc agtggccgtt cgtgctgcac gatgcccgcc acggcgccgt
     2221 cctgcagcca gggcagcgtc acccggatgc ggccctcggc cggtctcgcg acgtcgatct
     2281 gggccttgcg gaagacgccc tcggagacgt tggtgtcgat cgcggcccga agctcctggc
     2341 cgaggtcgaa caaccacttt tcctcgcgcg agagccggcg cggcagctcg ccctgcaggt
     2401 cggtggcacg ggtcgacgcc agtagccggg caccactgag gcggaggacc agcacgtgat
     2461 cgtcgcccgg cgaccgcgcc acccagtccg ccagcgccgc ctgcagcggc gcagtgtcgt
     2521 cgggattcgc cgcgacgatg cgcgcgacgg cctcggccag cgccgaggcc tcgaggatgg
     2581 ccatgcgacc ctgcgtctgg gccgccagct cgtcgcgcga ctggccgacg aaccagcggc
     2641 cgagcagcac ggtgacgagc agcgcgagca gcaggccgag cgccgccggc tgcagcagga
     2701 aacgccgcgc gcggcgggac ggcgtgtctg ccgccgcctg ccgccgggtg cgtacgttgc
     2761 ctgcaatctc gctcacgatt cagccctgct cagcgcccct tgcgcagctt cgcgatgttg
     2821 cccttgaccg tctcctgggc ttccttgagc gcggcctcgg gcgtcgccga acccttcacg
     2881 atcttgttca tcgcggtcgt gaccggtgac cacatcagcg tcatctcggc gtagttcggc
     2941 atcggcacgg ccgtgtcgag ctgcttgcgg aacccctgca gcgtcgggtt cttgacgacg
     3001 cgcggatcgg tatacacggc ccggttggtc ggcagctggc caccttcgag cgcgagcacg
     3061 acggccgcct cgggcgagat gaggtactcg gcgaacttgt acgcctgctc cttgtgctgg
     3121 gagccggcgg agacgtagac gccctcgacc gtgagccagg gcttcatcgg cttgccaccc
     3181 gcctcggaga cgagcggcag cggcgcgatc gcgtacttga ccttgtcctg cacctcgccg
     3241 aggaaccagg ggcccgagaa cacgatcggc gtcttgcctt cgttgaacag gctcgcgatc
     3301 agcgtcgacg agggatcggc gggcaggatc ttgtcctgct tgtaccactt gagcatcgtc
     3361 ttgacggccg cgacgttctg cggcgagtcg atggtcgggg tcgggccggg cgcgaacacc
     3421 tggccgccgt aggcgttcat cagggccgcg tggaagaagt agttggagta ctcgtacgcg
     3481 aggccgaagc gtccggtcga cacgtcggtc atgcccttgg ccagcttgac gaggtccgcg
     3541 gtggtctttg gcggcgactt gacgagcgcc gtgttgtaga tcatcgcgat cgacttgtag
     3601 ttgagcggca ggccgtagac cgtgtcgcga taggtcatcg cctccatcat ccccggcacg
     3661 agcgacttga cggtcgcgtc ctcgaggtag aagtcgatgg gctcgatcgt ctggccggcc
     3721 tcgacccagc cgccgagccg gtcctgcgcg aagatgaaga cgtccgggcc cttgccgcgc
     3781 ggtacggagg ccgtgatctt gtcggcgtac gcgtcgtagg gcacggcgag cgtcttgacc
     3841 gcgatgtcct tctgcttcgc attgaactgc gccacgacct tctcgaacgc ggccttttcg
     3901 tcgccgcggt aggcgtgcca gaccgtgagg tcggtggccg gccgcgcggc cgcaccgaaa
     3961 ccgaaggccg cgacggcaag tgccgcgccg aggccgcggc ggaggaagtg cctgcgaggg
     4021 atggtcgtcg tcatgctcga tgctccgttc gtgttcgcgc ggacctcagc ccgcgatgcg
     4081 ctgttccgta gccgggtcga agatgtgcat ggaatcggcg ttcagcacca ggtcgatctg
     4141 ctcgccgaac gccgggatcc ggtgcgcgcc gagcttggcg atgatcacgt cgtcgccgca
     4201 gcggaaatgc acgatcacct cgtgcccgat cgtctcgatg atctcgaccg ggccgccgag
     4261 cggcgcggtc agcgcccagt cgtgtgcccc cgcctcgccc acgtgctcgg ggcgtatgcc
     4321 gagcagcacc tcgcgcccgg cgtgggcgcg cagcgcgtcc cgcagggccg cgggcaccgg
     4381 ggtcgcgaat cctccggcgc gcagctgcgc gccgtcgccg tccacggtgg cgcgcaccat
     4441 gttcatgttg ggggtgccga tgaacttggc gacgaacacg ttctgcgggc ggtcgtacag
     4501 ctcgagcggc gtgccgacct gcttcaggtt gctcttgcgg tccttgccca tcggcgcgag
     4561 caccgcgatg cggtgcccca gcgtcatcgc ctcgacctgg tcgtgcgtga cgtagacggt
     4621 ggtcgtcttg agccgctgct gcaacttggc gatctcggcg cgcatctgca cgcgcagttc
     4681 ggcgtccagg ttcgagagcg gctcgtcgaa caggaacacc gacggcttgc gcacgatcgc
     4741 gcggccgagc gcgacgcgct gccgctggcc gcccgagagc gccttcggct tgcgatcgag
     4801 cagcggcttg agcccgagga tgccggccgc ctcgtcgacc agcttgtcga cgtcggcctg
     4861 cggcaccttg cggatcttga ggccgaacgc gatgttctcg cgcacggtca tgtgcgggta
     4921 cagcgcgtag ctctggaaca ccatcgcgac gtcgcgatcc ttcgggtgca cgtcgttcac
     4981 gacgcggtcg ccgatcgcga gctggccctc cgaaggttcc tcgagcccgg cgatcatgcg
     5041 cagcgccgtc gacttgccgc agcccgacgg cccgacgaac accatgaact cgtggtcgtg
     5101 gatctcgagg tcgaggttct cgatgacgct gacgtcgccg aagcgcttcg cgatgccctg
     5161 gaatgctatg cgtgccatga ctctcgcccg gccctcctcg ctatccggtc gtgcgacgga
     5221 cgtagacgcg tgcctgccgc gcaggcaccg agacggcgag ctgcccgccg gacaggccgg
     5281 ccgtctcgcc cgtgacgact tcggcgatcg cactgccctg ccaggcggac ggcgccggca
     5341 cggtggccgt ggcctccgcc cggccgcggt tcacggcgac cacgaccgcg ttgccggacg
     5401 ccgcgtcgac gcgctcgaac acgagcaggt cgccctccgc cgacagacga cggaagccgc
     5461 cttcggcgag cgcggggttg tcccggcggg cctggatcag cttgcggtag tactcgcgca
     5521 acgattcgtc gcgggcgacg ccgtggccgg gctgcacggg acgttcgccc cacggcatgt
     5581 cgttgcggtt ggtgggccag acgctgccct gacgggcgac ttcctcgccg tagtagatcg
     5641 tcgggatgcc gagcgaagtc agctgcagcg cgacgcagag ccggaacgcg tcgaggtcgc
     5701 cgcccaggtt gtgcagcgcc atcggttcgt cgtgcgacga gaggtagtgc gcgaggtgaa
     5761 aacccggtcg cacgttgtgg cgcttctcga ggtacgcggc gaaggcgacc gtgcgtccct
     5821 tgccctgcac gaacgcctcg cagctgccct tgaacgtgaa gtcgaagccg gcgtccatct
     5881 cgtcgccggc gaaatagtcg tcgagcacct gcgcggaacc accccagacc tcggcgagca
     5941 ggaagaagtc acggccgagc cggccgcggg cctcggcgcg atgcttctgc cagaacgcgt
     6001 ggtcgatgtg cttgacggtg tcgagccgga atccatcgag ccccgtacgc tgcgcgagcc
     6061 cgaggtgcgc gtcgaacagg tgccgctgca cctccggcag ttcggtccgg aggtccggca
     6121 ggccgccgac ctggcactgc agctcgtcga ccgcgcagtc gacgggcttg gtgcgtatcc
     6181 agccggcggt cgcgggattg ctgacgtact gcgcgtcgta gccgacgtgg ttgtagacca
     6241 cgtccagcag caccttgatg ccgcgcgcgt gcgccgcctc gaccagctgg cgcagttccg
     6301 cctcggtgcc gaagcgcggg tcgagctgcg tgtagtcgtc ggcccagtag ccgtggaacg
     6361 cgcagtgctc gaaccagccg cccggcacgg agacccccgt gggcggttgc gccggcgggc
     6421 agaagtcgat ctggcgcacg acgggcgtga tccagatggc cgtggcaccg agcgaggcga
     6481 tctcgtcgag ctgctgcgcg aggccggcga agtcgccgcc gtggaatccg ccggggttgg
     6541 cgcggtcgac gttggtgtcg ttggccgccg ccccgtctgc gtagcggtcg aggatcacga
     6601 agtagaggac ggcgtccgac cagttgcgtt gcggcgtcac cttgaccggg tcggtcgccg
     6661 ccatggcggt attcgtcgac ggcccgttcg tggcgcaccc ggccagcgcc aggccggcca
     6721 gcagcacgca agcggacgca tgcagaaggg ttttcgccga ggtggccccc ggtcgcttca
     6781 tctccgtcct ttgctcactc cgtgtgcgca tgaccgagtt cgagctggtg ttgtagttct
     6841 gccgcagccg gtgtcggagg gccccggcag ccttgcacgg tgcattgcaa aaaagcaatc
     6901 ctgaacaatc agccggaaac ggcggccgac gcgcctggag ccggcgcggg cccgatcaga
     6961 agagcgtgta gatcagcgag gcgagcgccg actgctggct gaccaccacg agtccgccga
     7021 gcgccaccag cacgaggatg atcggcagca gccagtactt cttgcgcacg cgcatgaact
     7081 gccagaggtc gcgcaggaat tcacccatca gaacggatcc tcgagtcgca agcggtgctc
     7141 cggtggcacg ggcacgcggt aggtatccac cgccgcatcg aagccggtca cgtactgcag
     7201 cttgccgaga cgccgcagca cccagcccac cggaagcagg atcgcaaaaa agaccgtacc
     7261 gagcagcagc caggtgttga gccagccgag gacgcgggca accggcagga acgcgcgatg
     7321 gatcggcagg atggccggcg ggtacatcca ggccgtgacg acgagcaccc acgcgacgat
     7381 cagtggccac agcggggccg tccggtcccg gagccacggc atcagccacc agaacacgac
     7441 cacgatcacc agcgcgaggc cgagcgcaaa gaaccgcagt tcgcgccggt cgtgacgggt
     7501 gagtgcccgg ggtccccgat gcatctctcc cgacacctcg cgagagtgct gggccgacac
     7561 ccctcccgat tcgtcgggag aggtcgaccg caggtcgcgt gagggcgctt tgttcatctc
     7621 gagtttcagt cggcctcgaa ttcgcgcacg gcgtgttcgg tcagcccgtg agacgcgggt
     7681 tgcagccgtc ggtccagcac gtgacgcccc agcacgaggt agtccatcgc ggtggccatg
     7741 aagcaccggt aggcatcggc gggcgtgcag acgatcggct cgccgcgcac gttgaacgac
     7801 gtattgacca gcaccggcac gccggtcttc tgctcgaacg cctcgatcag gcggcggaaa
     7861 cgggggttcg taccggcgtc gaccgtctgc acccgcgcgc tgtagtcgac gtgcgtgatc
     7921 gtcggcaggg ccgactggac ctggcgcagc cgctcgaagc cgtcaccgga cgccgcggcg
     7981 ccctcgggcg gacggtccgc cagcggcgcg acgatgagca tgtacgggct cggccggtcg
     8041 agttcgaaca cctcggcggc gcgttcctcg agcacggccg gcgcgaacgg ccggaacgat
     8101 tcgcggaact tgatcttgag gttgagcgct cgctgcatgt cgggccgccg cgggtccgcg
     8161 aggatcgatc gcgcaccgag cgcgcgcggg ccgaattcca tgcggccctg catccacccc
     8221 accaccgcgc cgtcggccaa cagcgcggcc acgcgctcgc cgagggccgc gtcgtccggc
     8281 agcgtttcgt gcggcaggtc ctggtcgcgc aggaaggcct cgagcgcctc gtcgccggtc
     8341 tcggggccga gcagggcgcc cgacatcgca tcgggatgcg cgggcaaccg cgcagcgtcc
     8401 gcgttctttg cgtaccaggc gacgagcgcc gcgccgagtg cgcccccggc atcgccggcc
     8461 gcgggctgga tccagacgtc gtcgaacacg cgctcacgga ccagccggcc gttggtcacg
     8521 cagttgagcg cgacgccgcc ggcgaggcag aggcttcgcg ctccggtctc cacgcgcagc
     8581 gtcttcgcga ggcgcagcac gatctcgtcg gtgacctgct ggatcgacgc ggccacgtcc
     8641 atcgtgtgct gcacgatcgg ctgctcagga tcacgcggcg gtgcgccgaa cagtgcgtgg
     8701 aagcgctcgc tggtcatgcg caggcccgtc gcatagtcga agtagcgcat gtcgagccgg
     8761 aaggtgccgt cgggcttgac gtcgacgagg tgctcgcgga tcgcgccggc gtatcgcggc
     8821 gtgccgtatg gcgcgagtcc catcatcttg tactcgcccg agttcacctt gaaccccgtg
     8881 tagtaggtga acgccgagta caacaacccg agcgaatgcg ggaagtgctg ctcccagagc
     8941 ggttgcagcg aggtgccctg gccgaaccag gccgacgtcg tcgcccactc gccgacgccg
     9001 tcgaggcaca gcaccgcggc cgattcgaac gggctgggat agaacgccga cgccgcgtgg
     9061 gagcgatgat gctcgctgaa gagcagcggc gggggcgtgc cccacggtcc gcacagttcg
     9121 tcgagcctgc gccgcagttc cgacttgagg aacagcttct ccttgagcca caccggcatc
     9181 gaggcgacga acgagcgcag gccgtgcggt gcgctcgcga ggtaggtctc gagcagtcgc
     9241 tcgaacttga ggaatggctt gtcgtagaac acgacggcgt cgaggtcgcg caggcgcagc
     9301 cccgcgtgcg cgaggcagaa acgtgcagcg tgcgccggga aaccggcgtc gtgcctgcga
     9361 cgcgtgaagc gctcttcctg cgcggcggcc acgatgcggc cgtcgacgac cagggccgcg
     9421 gcagcgtcgt gataatatgc cgagagtccg a
//
LOCUS       DASPFR010000242        13072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1778567, whole genome shotgun sequence.
ACCESSION   DASPFR010000242 DASPFR010000000
VERSION     DASPFR010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13072
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1778567"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..2311)
                     /locus_tag="VD737_06315"
     CDS             complement(104..2311)
                     /locus_tag="VD737_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXW10208.1"
                     /translation="MSTIRKQLAGVQVLGALVTASAMAQEATPAPQSGPVLEEIVVTA
                     DRQDSYGADFVQAGSFRGARQIDTPLTVSVIPEQLLKSQQALNIMDALRNSPGVTSSQ
                     TSPTVYNNLSIRGIPVENRGNYRMNGSLPIVNLIDLPLENKVRIEALKGASALYYGFT
                     TPSGIVNLTTKRPPPEPVLSVAVNANEHGQVQGHVDAGSTWGKFGVRGNLVHGSVDSG
                     IDNTSGDRSFAALALQYDATEDVQLNLDAEYIEKEVTEPTVLQGPTAAASLLTIKPKL
                     PDPANNPGTEGFMNRADETNILGRVAWKLNQSWGLTAEGGISYAERDRRFSTLSNFNP
                     TTGLGTLTASAADDQLYRNKNARLELAGAFATGPVAHELLLGASTNIRRQYSAPAVAS
                     VGTSSATTRANCVRLGLADTCLQSAYDPIDLADIRFDGSAPYNPSRDTAIEDTGIYAF
                     DRMQFGGENADMISVLAGVRKSFYKETQEGPNATTKDEFETFSDEPVAVSGGLVYKPA
                     TWASIYGTYIEGLESTPAAPLTTVNAGQVLPASESKQYEAGVKLEPRKGLLFTAAYFD
                     IERQLTYTNAANVFVQDGEATYKGFETSLTGEITPDLSIYASALWLDAEQGETSDPAL
                     IGNQIENTAEEQWSIAAEYRLSSIAEGLSINAGVFRTGERPINPQNSLFLPGYTLFDI
                     GAAYAFDLGGVRMTARLNAQNVTDERYFVSTAGNYIAYGPPSVIKLSVTASIF"
     gene            2537..4069
                     /locus_tag="VD737_06320"
     CDS             2537..4069
                     /locus_tag="VD737_06320"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXW10209.1"
                     /translation="MTRRRFTGLAVGSAAGLLAAPRMFGAWSAEAVAAPPGDGLPRER
                     PEARGVDPCAIVGFLDEVAGAGLELHSFMLARGGDVIAEAWWEPYRPDRVHMMHSLTK
                     SVAVSGVALALKERLFSIDDKVISFFPDERPAQVSDHLAAMTVRDLLTMRTGHENEIS
                     GSVWRQIRTSWVAEFFKVPVVHRPGTRFVYSSAASFMLSAIVTKVTGQTLRDYMEPRF
                     FRPLGISGLSWDVGPGGINPGGNGLSWKTADSLKLGMLYAQQGRWNGQQILTPDWVRE
                     ATRPQVAEGEYGYQWWIGPGKAFYAVGLFTQMSIVFPEHDAVLAVTSAIDESEQLTPI
                     VWKHFPAAFAGERRPADAAAHAALGERTRNLSVLPSLGAASSDLAGRISGKPYRARPN
                     AEAIESLRFDFAGDRCRFTLRDARGEHAVDVGLGRWIEGRTSMTGNKLHHQYQPESML
                     VVAGGQWTAPDTFQMTWQFAETAFRDTVVCRFADAAVTLDRSVNINSAATSLPTVYAA
                     RA"
     gene            4158..5555
                     /locus_tag="VD737_06325"
     CDS             4158..5555
                     /locus_tag="VD737_06325"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXW10210.1"
                     /translation="MLLAAAAMQAHAADPVADIARAQEARFREVYRELVETDTSLSTG
                     NCTLAAQKMAARLAAAGHPASRVRVVVPPRFPKQGNLLATIPGADEQLPALLLLAHID
                     VVEAKASDWKRDPYRLVEEDGWFYARGAIDDKAMAASFVDAFVRYAEEGFRPRRTLKL
                     ALTCGEETDATFNGVQYLLETEPGKLAAGFAINEGGSGALDAAGKPVSFGVQVGEKIY
                     QDFTLVATAPGGHSARPTDDNAIGRLAAALVRVDAHRFPIHMTDATRTFFARSAAVHE
                     GQLAIDLERVGNGTADAAAFERVAGALPLWNAYLRTTCIPTQVVGGHAPNAQPQRAEA
                     TVNCRIMPGERIDEVRARLVEVVGDSALEIRLAAEPGPQPPAPPLDPRLMRHVEGLVS
                     EMWPGIPVILAISTGATDGRFLNAAGIPTYGVSGLFRDPDGNGVHGLDERVRVRSLLD
                     ARAFLYRLVRGLATD"
     gene            5555..6670
                     /locus_tag="VD737_06330"
     CDS             5555..6670
                     /locus_tag="VD737_06330"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HXW10211.1"
                     /translation="MSPNSRATLLKLHRWVGLSLAVLLLVQGTTGSLLVFRDEIDRAM
                     HPELTLAAAGERRPVQQLMDLVRASAPGATLQRVKVPEDPAHAWMFFMRLQSDAPYMV
                     AVDPYRGEILRQGSYLAWPSEWLFRLHDALLAGRTGEIVVSVEGIALVFLAVFGVIVW
                     WPGRRRLKSGFRILTGQGADRAVRTSHRAVGAAVAIVLTMSGTTGALLIHRAALQEYL
                     PMTPRPKFVVAPQDRAVLPVDALLDRARATHGPLPLRELRFSGAAGQVVALYFQDETS
                     LRRNATRQYFYDAYAGRELGRYEPDAVPPLNTAYDWLYTIHTGRAGGWAGRAVIQCAG
                     LSLVFFSVSGVWLWFSARRQRRLRKLQAQARMARGPA"
     gene            complement(6713..7201)
                     /gene="smpB"
                     /locus_tag="VD737_06335"
     CDS             complement(6713..7201)
                     /gene="smpB"
                     /locus_tag="VD737_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HXW10212.1"
                     /translation="MASPSKQKDGKSAPNPSIAQNKRARFDYFIEEKLEAGLALQGWE
                     VKSLRAGKAQITEGYVIVRQGEAFLIGAHVTPLRTVSTHVVADPTRTRKLLLHRRELD
                     RLIGAVERKGHTIVPLELYWKDGKVKLAIGLAKGKQAHDKRSTEKDRDWQREKARLLR
                     KR"
     gene            7278..7721
                     /locus_tag="VD737_06340"
     CDS             7278..7721
                     /locus_tag="VD737_06340"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HXW10213.1"
                     /translation="MSIHEVKRTALVTYTPEQMFDLVVDVERYCEFLPWVKSAHVHEK
                     TERDLKASLTMERAGVKQTFTTRNEMERPDWMSLTLVEGPFRTLDGLWTFTPIGDAGT
                     RVVLDMKFEFSNAIASMLFGKAFEHSIGQLIDAFVARAKTSYGTP"
     gene            7708..8064
                     /locus_tag="VD737_06345"
     CDS             7708..8064
                     /locus_tag="VD737_06345"
                     /inference="COORDINATES: protein motif:HMM:NF002490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HXW10214.1"
                     /translation="MEPPESPPPPGRAGSSSHPPPAARVEVACALPERQRVVTLELPV
                     EGLTAAQAVQRSGLLQEFPQIAEHPLALAVFGVTCELERPLQCGDRVEILRPLRHDPR
                     ALRRERAASSRKPRRR"
     gene            complement(8074..8535)
                     /locus_tag="VD737_06350"
     CDS             complement(8074..8535)
                     /locus_tag="VD737_06350"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HXW10215.1"
                     /translation="MKRFRINVHARAAVVLASLMGAVLTTGGCVYRPNIQQGNLLEVE
                     DVEKVETGMTRSQVRYLLGTPMLADPFDPQRWDYIYTFRRGRESRIDRSHFVVHFDGD
                     KVSKVEKLDLPDGANDPKRRKPQADAEPRDPPPLPTIDQPQPDAPRPGDND"
     gene            8631..9062
                     /gene="fur"
                     /locus_tag="VD737_06355"
     CDS             8631..9062
                     /gene="fur"
                     /locus_tag="VD737_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013465004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HXW10216.1"
                     /translation="MEPSQLRRAGLKITAPRLKILEILASATVRHMSAEDVYRRLLEL
                     NEDIGLATVYRVLTQFESAGLVTRHHFEGGTAVFELNEGTHHDHIVCVDCGHVEEFSD
                     EDIEERQRSIADRLGFEITDHSLTLHGHCVRESCPYRPKRP"
     gene            complement(8989..10752)
                     /gene="recN"
                     /locus_tag="VD737_06360"
     CDS             complement(8989..10752)
                     /gene="recN"
                     /locus_tag="VD737_06360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HXW10217.1"
                     /translation="MLTHLQIRDFAIVEAVELEFARGFTVLTGETGAGKSILVDALML
                     AVGGRADSGAVRHGAERAEVSATFDVRGNAQALAWLEDQSIGHDGECQMRRVVAADGR
                     GRAYLNGQVVPVQSLRALGEFLVDVHGQLEFQALARRGYQRETLDGSGRLEAGLEAVR
                     SAYVDWHALDTRRSTFEQRLRDRTSRLDLLQHYAGEFAALDPKPGEAEALADERKRVA
                     SLGRLADGTAQVEALLGGDDGGITAALARSQSVLRGLVAIDGALGSAAAQLEEASIAA
                     REALTTLRRYVDSLEADPARQEWVESRLAALEAVARKHRVEPAELPAVRARLDAELAE
                     LESGALTEAELLQRLEAARRRYVQVADTLTAARRRAAQALDGRVTGFMQSLGMPGGVF
                     STRVEPHDPPQFSAIGNDDVEFLVSANPGQPPRPLAKVASGGELSRISLALQVAAVEA
                     AHLPCLVFDEVDAGVGGAVAEMVGRQLHALAGRGQVLCVTHLPQVTCQADHQLRVSKV
                     VAAGSTRTRIETLDDAARIEELARMLGGAQITARTREHAEEMLASGRRPATATKPAKA
                     VSADKGSSRGRSGRAGSASGR"
     gene            complement(10759..11667)
                     /locus_tag="VD737_06365"
     CDS             complement(10759..11667)
                     /locus_tag="VD737_06365"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HXW10218.1"
                     /translation="MEQRATDRTCVALAGRAADPRVGEAMRVLASHLLARGCLVKVAA
                     AEELVLTRDGVRTVLEPDLAMDADLVVAVGGDGTMLHAARMAALADVPVLGVNRGRLG
                     FLADVGPDRMIESVEDALAGRCLAERRMLLAAHLMAEGRPIDSLGLNDVVVTKRETGR
                     TVDVRTWVNGVYVNTHGGDGFIVATPTGSTAYALSCGGPIVHPSLDAIVLVPVSPHTL
                     SDRPIVVPAGSLIEIELADRFDTRAQVACDGHVLCDLEPGVRLRIERAKVTATLLHPP
                     GHDYYRILRSKLHWGRGTREGQPPGA"
     gene            11784..12851
                     /gene="hrcA"
                     /locus_tag="VD737_06370"
     CDS             11784..12851
                     /gene="hrcA"
                     /locus_tag="VD737_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HXW10219.1"
                     /translation="MSDGQEDQLNERARHLLRVLVESYIREGQPVGSRSLSRDSKLNL
                     SAATVRNVMADLEEYGFVSSPHTSAGRVPTDKGYRFFVDTLLRQRQMASVENVIAELQ
                     QRLEMQEDRDPKALVAVASQVLSSITHLAGVVTIPRQPHQALSQIEFLPLSENRVLAV
                     MVVNGRDVQNRIVHLERYYSAEELRRAATFLNQQFAGKELNQVRTDIVTELTQTGETL
                     NQLMRDAIRVAQQVVGEGSRSGEAEYVIAGETNLMEFAELSNVEKLRRLFDAFTAKRD
                     ILHLLDQSLRADGVQIFIGQESGYQILDDISLVTAPYALDNETVGVLGVIGPTRMAYD
                     RVIPIVDLTARLLGSALNSRR"
BASE COUNT         1900 a         4502 c         4571 g         2099 t
ORIGIN      
        1 ggtgcaccca cggccgcctg tccagcgtgc ttcccagcag gcccggcaaa tcgacgactc
       61 gactgccgtg cacagtcacg cggcgggacg ccggacgtcg agatcagaag atgctggccg
      121 tcacggagag cttgatcacc gaaggtggcc cgtacgcgat gtagttgccg gcggtcgaca
      181 cgaagtagcg ctcgtcggtg acgttctgcg cattgagccg cgccgtcatc cgcacgccgc
      241 cgaggtcgaa ggcgtacgcg gcgccgatgt cgaacagcgt gtagcccggc aggaacagcg
      301 agttctgcgg attgatcggc cgctcgccgg tgcggaacac gccggcgttg atcgagagcc
      361 cctcggcgat gctcgacagc cggtattcgg cggcgatcga ccactgctcc tcggcggtgt
      421 tctcgatctg gttgccgatc agcgcggggt cgctggtctc gccctgctcg gcgtcgagcc
      481 acagtgcgga ggcgtagatc gacaggtcgg gcgtgatctc gccggtcagg ctggtctcga
      541 agcccttgta cgtggcctcg ccgtcctgca cgaacacgtt ggccgcgttg gtgtacgtga
      601 gctggcgctc gatgtcgaaa taggccgcgg tgaacagcag gcctttcctc ggctcgagct
      661 tgacaccggc ctcgtactgc ttggattcgc tggccggcag cacctggcca gcgttgaccg
      721 tggtgagcgg cgccgccggt gtcgattcca ggccctcgat gtaggttccg tagatgctcg
      781 cccaggtcgc gggcttgtag acgaggccgc cggacacggc gaccggttcg tcggagaacg
      841 tctcgaactc gtccttggtc gtggcgttgg gaccttcctg cgtctccttg tagaaagact
      901 tccggacgcc cgcgagcacg ctgatcatgt cggcgttctc gccgccgaac tgcatgcggt
      961 cgaacgcgta gatgccggtg tcctcgatcg ccgtgtcgcg cgaggggtta tacggggcgg
     1021 acccgtcgaa gcggatgtcg gcgaggtcga tcgggtcgta ggcgctctgc aggcaggtgt
     1081 cggccagtcc gagccgcacg cagttcgcgc gcgtcgtggc agaactcgtc ccgacgctcg
     1141 ccaccgcggg cgcggagtac tggcggcgga tgttggtcga ggcgccgagc agcaactcgt
     1201 gcgcgaccgg gccggtcgca aacgcgccgg ccagttcgag gcgtgcgttc ttgttgcggt
     1261 acaactggtc gtccgccgcg ctcgccgtca gcgtgcccag gcccgtcgtc gggttgaagt
     1321 tggacagcgt gctgaaacgc cggtcgcgtt ccgcgtacga gatcccgccc tcggcggtca
     1381 agccccacga ctggttcagc ttccacgcga cgcggccgag gatgttcgtt tcgtcggcgc
     1441 gattcatgaa gccttcggtg ccggggttgt tcgcggggtc cggcagcttc ggcttgatcg
     1501 tcagcaggct cgcggccgcc gtcggcccct gcagcacggt cggttccgtg acttccttct
     1561 cgatgtactc ggcatcgagg ttcagctgca cgtcttcggt ggcgtcgtac tgtaaggcga
     1621 gggcggcgaa gctgcggtcg ccactcgtat tgtcgatgcc cgaatcgacg ctgccgtgca
     1681 ccaggttgcc gcgtactccg aacttgcccc aggtcgagcc ggcgtcgacg tggccctgca
     1741 cctgcccgtg ctcgttggca ttgaccgcca cgctgagcac cggctcgggc ggcggccgct
     1801 tggtcgtcag gttgacgatg ccggagggcg tcgtgaagcc atagtagagc gcggatgcgc
     1861 ccttcagcgc ctcgatgcgc accttgttct cgagcggcag gtcgatcagg ttgacgatcg
     1921 gcagcgagcc gttcattcga taattgccgc ggttctccac cggaatgccg cggatggaca
     1981 ggttgttgta gacggtggga ctcgtctgcg agctcgtgac gccgggcgaa ttgcgcagcg
     2041 catccatgat gttgagcgcc tgctgcgact tcaggagctg ttccgggatc acgctcaccg
     2101 tgagcggcgt gtcgatctgc cgcgcgccgc ggaagctgcc ggcctgcacg aagtccgcgc
     2161 cgtagctgtc ctgtcggtcg gcggtgacga cgatctcctc gagcacgggc cctgactgcg
     2221 gggccggcgt cgcctcttgc gccatcgccg acgccgtgac cagcgcaccg agcacctgga
     2281 cacctgcgag ctgcttgcga atcgtggaca tctggcgatc ccccgtaatg ctgccgcccg
     2341 tcgggcggac gatacttcga cgtcggggcg aagttaccct cgtcaagctg tcacggacct
     2401 gtcccgccgt gtcgcgcgtt cgcgacacgg cacgcggtga cagcgtggcg acagattgct
     2461 ttcctaggct cacacgcagg acggcttgat gaccgagcgt gcaccagaca tgactgcgac
     2521 aggtttccgc cggcccatga cgcggcgaag gttcacgggg ctggcggtcg gcagcgccgc
     2581 gggtctgctc gccgccccac gcatgttcgg ggcctggagc gccgaggccg tcgccgcgcc
     2641 gccgggcgat ggactgccac gcgagcggcc cgaggcgcgc ggcgtcgatc catgcgcgat
     2701 cgtgggattc ctggacgagg tcgccggcgc cgggctcgaa ctgcacagct tcatgctcgc
     2761 gcgtggcggg gacgtgatcg ccgaggcctg gtgggagccg tatcggcccg accgcgtcca
     2821 catgatgcat tcgctcacca agagcgtggc ggtgtccggt gtcgcgctcg cgctcaagga
     2881 aaggctgttc tcgatcgacg acaaggtgat ctcgttcttc ccggacgagc ggcccgcgca
     2941 ggtcagcgac cacctggccg cgatgacggt gcgcgacctg ctgacgatgc gcacgggtca
     3001 cgagaacgag atctcgggct cggtctggcg gcagatcagg acgagctggg tggcggagtt
     3061 cttcaaggtg ccggtcgtgc accgcccggg cacccgcttc gtgtactcca gtgccgcgag
     3121 cttcatgctg tccgcgatcg tgaccaaggt gaccggccag acgctgcgcg actacatgga
     3181 gccccgcttc ttccggccgc tggggatctc gggcctgtcg tgggacgtgg ggccgggcgg
     3241 gatcaatccc ggcggcaacg gcctcagttg gaagacggcc gattcgctga agctcggcat
     3301 gctctacgcg cagcagggtc gctggaacgg gcagcagatt ttgacgccgg attgggtccg
     3361 ggaggcgacg cgcccgcagg tcgcggaagg cgagtacggc taccagtggt ggatcggtcc
     3421 gggcaaggcc ttttatgcgg tcgggctgtt cacgcagatg tccatcgtct ttccggagca
     3481 cgacgcggtg ctcgccgtca cgtcggccat cgacgagagc gagcaattga ccccgatcgt
     3541 ctggaagcac ttcccagcgg ccttcgcagg ggaacgccgg ccggccgacg ccgccgccca
     3601 tgctgcgctg ggcgagcgca cccgcaacct ttccgtgctg ccgagtctcg gcgccgcctc
     3661 gtcggacctc gcggggcgca tctcgggcaa gccctaccga gcacgaccca acgcggaggc
     3721 catcgagagc ctgcgcttcg acttcgccgg cgatcgctgc cgcttcacgc tgcgcgacgc
     3781 gcgcggggaa cacgcggtcg acgtcggcct cggtcgctgg atcgagggcc gcaccagcat
     3841 gaccggcaac aaactgcacc accagtacca gcccgagtcg atgctcgtcg tggccggcgg
     3901 gcagtggacc gcgcccgaca ccttccagat gacctggcag ttcgcggaga ccgcgtttcg
     3961 cgacacggtg gtgtgccgct tcgcggacgc cgccgtgacg ctcgaccgca gcgtcaacat
     4021 caactcggcg gcgacgtcgt tgccgaccgt ctacgctgcg cgagcctgag gcggccggac
     4081 caatggacat cggtcgccgt gcgcgtcgca gtctcgcccg ccgcatcgcg gccgtggcga
     4141 ggcggtgtcg atgtggcgtg ctcctggccg cggcagcgat gcaggcgcac gccgccgacc
     4201 cggtcgccga catcgcccgc gcgcaggaag cacggtttcg cgaggtctat cgcgaactcg
     4261 tggaaaccga cacgtcgctc tccaccggca actgcaccct cgccgcgcag aagatggccg
     4321 cgcggctcgc ggccgcgggt catcccgcca gccgggttcg ggtcgtggtc ccacctcgtt
     4381 tcccgaagca gggcaatctc ctcgccacga tccccggtgc ggacgagcag ttgcctgcgc
     4441 tgctgctcct cgcacacatc gacgtcgtcg aggcgaaggc ctccgactgg aaacgcgatc
     4501 cgtaccggct ggtcgaggaa gatggctggt tctacgcgcg cggcgcgatc gacgacaagg
     4561 ccatggccgc gtcgttcgtc gacgcgttcg tgcgctatgc ggaagagggc ttccggccgc
     4621 gccgcacgct caagctggca ctcacctgcg gcgaagagac cgatgcgacg ttcaacggcg
     4681 tgcaatacct gctcgagacg gaacccggca agctcgccgc gggattcgcg atcaacgagg
     4741 gtggctccgg cgccctcgat gccgcgggca agcccgtctc gttcggggtg caggtcggcg
     4801 agaagatcta ccaggacttc acgctggtcg cgaccgcccc gggcggccac agtgcccggc
     4861 cgaccgacga caacgcgatc ggccgcctgg ccgcggcgct ggttcgcgtg gacgcgcatc
     4921 gttttccgat ccacatgacc gacgccaccc ggacgttctt cgctcgctcg gccgcggtcc
     4981 atgaaggcca gctcgcgatc gatctcgaac gggtcggcaa cggcacggcc gacgcggcgg
     5041 cgttcgagcg tgtcgcgggc gcgctgccgc tctggaacgc gtacctgcgc acgacgtgca
     5101 tcccgacgca ggtggtgggc gggcatgcgc cgaacgccca gccgcagcgt gccgaggcca
     5161 ccgtgaactg ccgcatcatg cccggtgagc gcatcgacga ggtgcgcgcg cgactcgtcg
     5221 aagtcgtggg cgattccgcc ctcgagatcc ggctggcggc ggaaccgggc ccccagccgc
     5281 cggcgccgcc gctcgacccg cgactgatgc ggcacgtcga gggcctcgtg tccgagatgt
     5341 ggcctgggat tccggtcatt ctggcgatca gcacgggtgc gacggacggc cggttcctga
     5401 acgcggccgg catccccacg tacggagtct cggggctgtt cagggacccc gacggcaacg
     5461 gcgtgcacgg gctcgacgaa cgcgtacgcg tccgctcact gctcgatgcg cgcgcgttcc
     5521 tctaccgcct cgtccggggc ctcgccaccg actgatgtcg ccgaactcgc gcgccacgct
     5581 gctgaaactg caccgctggg tcgggctgtc cctcgcggtg ctgctgctgg tccagggaac
     5641 gacgggttcg ctgctggtgt tccgcgacga gatcgaccgc gcgatgcacc cggagctgac
     5701 gctcgccgcc gccggggagc gtcgaccggt gcagcaactc atggatctgg tgcgtgcgtc
     5761 ggccccgggc gcgacgctgc aacgggtcaa ggtgcccgaa gatcctgctc acgcctggat
     5821 gttcttcatg cgcctgcaga gcgatgcgcc gtacatggtc gccgtcgatc catatcgagg
     5881 cgagatcctg cggcagggca gctacctcgc gtggccgagc gagtggctgt ttcgcctgca
     5941 cgacgcgctg ctcgcgggca ggaccggcga gatcgtcgtc agcgtcgagg gcattgcgct
     6001 cgtgttcctg gccgtcttcg gcgtgatcgt ctggtggccg ggccggcggc gactcaagtc
     6061 cgggtttcgc atcctcacgg ggcagggtgc cgaccgcgcc gtgcgcacca gccatcgcgc
     6121 ggtcggcgcc gccgtcgcga tcgtgctcac gatgtcgggg acgaccgggg cactgctgat
     6181 ccaccgcgcg gccctgcagg agtacctgcc gatgacgccg cgcccgaaat tcgtggtcgc
     6241 gccgcaggat cgcgccgtgc tcccggtgga cgccctgctg gaccgcgcgc gggcgacgca
     6301 cggtccgctg ccgctgcgcg aactgcgatt ctccggtgcc gccgggcagg tggtggcgct
     6361 gtacttccag gacgagacgt cactgcggcg caacgcgacg cgacagtact tctacgacgc
     6421 ttatgcaggc cgcgaactcg gtcggtacga acccgacgcg gtgcccccgc tgaacaccgc
     6481 ttacgactgg ctctacacga tccatacggg acgggccggt ggctgggcgg gacgcgccgt
     6541 catccagtgt gccggcctgt cgctggtgtt cttctccgtg agcggcgtgt ggctgtggtt
     6601 ctcggcgcgt cgccagcgcc gcctgcgcaa gctgcaggcc caggctcgca tggcgcgcgg
     6661 cccggcctga ggggccgcga cgcgcgcggg gaacgctcgt gggcaggggt ggctatcgct
     6721 tgcgcaacag ccgcgccttc tcgcgttgcc agtcgcgatc cttttcggtg ctgcgcttgt
     6781 cgtgcgcctg cttgcccttg gccagcccga tcgcgagctt gaccttgccg tccttccagt
     6841 acagctcgag cggcacgatc gtatggccct tgcgctcgac ggcgccgatc agccggtcga
     6901 gctcgcgtcg gtgcagcagc agcttgcgcg tgcgcgtcgg gtccgcgacg acgtgcgtcg
     6961 acaccgtgcg cagcggcgtc acgtgcgcgc cgatcaggaa tgcctcgccc tgcctgacga
     7021 tcacgtagcc ttcggtgatc tgcgccttgc cggcacgcag gctcttgacc tcccacccct
     7081 ggagcgcaag ccccgcctcg agcttttcct cgatgaagta gtcgaaccgc gcgcgcttgt
     7141 tctgcgcgat cgacggattc ggggcggact tgccgtcctt ctgcttgctg gggctggcca
     7201 tggggtcgtt gctgcgcggg tcgcgcattc tagccgcccc gcttacactg cgcccgtccc
     7261 gatcgtcccg gtcctgcatg tccatccacg aagtcaagcg caccgcactc gtcacctaca
     7321 ctccggaaca gatgttcgac ctggtcgtcg acgtcgagcg ttattgcgag ttcctgccct
     7381 gggtcaagag cgcccacgtg cacgaaaaga ccgagcgcga cctcaaggcc agcctgacga
     7441 tggagcgcgc tggcgtgaag cagacgttca cgacgcgcaa tgagatggag cgaccggact
     7501 ggatgtcgct gacgctcgtc gaagggccgt tcaggacgct cgacggactg tggaccttca
     7561 cgccgatcgg ggacgccggc accagggtcg tgctcgacat gaagttcgag ttctcgaacg
     7621 cgatcgcctc gatgctgttt ggcaaggcgt tcgagcacag catcggccag ctgatcgatg
     7681 ccttcgtcgc cagggcgaag acgagctatg gaaccccctg agtcgccgcc accgcccggg
     7741 cgggcaggca gctcgagcca cccgccaccc gccgcccggg tcgaagtcgc ctgcgcgctg
     7801 cccgagaggc agcgcgtcgt gacgctggaa ctgcccgtcg agggactcac ggccgcgcag
     7861 gccgtacagc gctccgggct gctgcaggag tttccgcaga tcgcggagca cccgttggcg
     7921 ctcgccgtgt tcggcgtgac ctgcgagctc gagcgaccgc tgcagtgcgg agatcgagtc
     7981 gagatcctgc gcccgttgcg gcacgatccg cgcgcgctgc ggcgcgaacg cgcggcctcg
     8041 tcgcgcaagc cgcgtcgtcg ctgagcgcga accctagtcg ttgtcgccgg ggcggggtgc
     8101 gtccggctgc ggctggtcga tggtcggcag cggcggcggg tcgcgcggct cggcgtccgc
     8161 ttgcggcttc ctgcgcttcg ggtcgttggc gccgtccggg aggtcgagct tctcgacttt
     8221 gctgaccttg tcgccgtcga agtgcacgac gaaatgcgag cggtcgatcc tcgattcgcg
     8281 gccgcgacgg aacgtgtaga tgtaatccca gcgctgcggg tcgaacgggt cggccagcat
     8341 cggcgtgccg agcaggtagc gcacctggct gcgggtcatg ccggtctcga ctttctcgac
     8401 gtcctcgact tcgagcaggt tgccctgctg gatgttgggg cggtatacgc agccgccggt
     8461 cgtgagcacc gctcccatga gcgaggccaa aaccacggcg gcgcgcgcgt gcacgttgat
     8521 gcggaatcgt ttcatgctgt ctggagttac cgggctgggc cggtgataat gcgtcgcgat
     8581 cataccgcaa ccgcgtgcga gcgcggcatc ctggccacag ggggcacacc gtggaaccgt
     8641 cgcaactgcg tcgcgctggg ctcaagatca ccgcgccgcg gctcaagatc ctcgagatcc
     8701 tggcgagcgc gaccgttcgt cacatgagtg ccgaggacgt ctatcgccgg ctgctcgagc
     8761 tcaacgagga catcggcctg gcgacggtct accgcgtgct cacgcagttc gaatcggccg
     8821 gcctcgtcac gcgccaccac ttcgagggcg gcacggcggt gttcgagctc aacgagggca
     8881 cgcaccacga ccacatcgtc tgcgttgact gcgggcacgt cgaagagttc tcggacgagg
     8941 acatcgaaga gcgccagcgc tcgatcgcgg accgcctggg gttcgagatc accgaccact
     9001 cgctgaccct gcacggccac tgcgtccgcg agagctgccc ttatcggccg aaacggcctt
     9061 agccggcttc gtcgccgtcg cgggccgccg gcccgacgcc agcatctcct ccgcgtgttc
     9121 gcgcgtgcgc gccgtgatct gcgcgccgcc gagcatgcgt gcgagttcct cgatccgcgc
     9181 tgcatcgtcc agcgtctcga tgcgcgtgcg cgtgctgccg gccgcgacca ccttcgagac
     9241 gcgcagctgg tggtcggcct ggcaagtcac ctgcggcagg tgcgtcacac agagcacctg
     9301 gccccgcccc gcgagcgcgt gcagctgacg accgaccatc tccgcgaccg cgccgccgac
     9361 accggcgtcc acctcgtcga acaccaggca gggcaggtgc gcggcctcga ccgcggccac
     9421 ctgcagcgcc aggctgatgc gcgagagctc gccgcccgag gccactttcg cgagcgggcg
     9481 tggtggctgc ccgggattcg cgctcaccag gaactcgacg tcgtcgttgc cgatggccga
     9541 gaactgcggc gggtcgtgcg gctcgacccg cgtcgagaac acgccgcccg gcatgccgag
     9601 cgactgcatg aagccggtga cccggccgtc gagcgcctgg gcggcgcggc gccgcgccgc
     9661 cgtcagcgtg tccgccacct gcacgtatcg tcggcgggcg gcctcgagcc gctgcagcaa
     9721 ctcggcctcg gtgagcgcac ccgactcgag ttcggccagt tcggcgtcga gcctggcacg
     9781 cacggcgggc aactcggcgg gctccacccg gtgcttgcgc gcgacggcct ccagcgcggc
     9841 gagccgcgat tccacccact cctggcgtgc ggggtcggct tccagggagt cgacgtaacg
     9901 ccgcagggtc gtgagtgcct cgcgtgccgc gatcgaggcc tcctcgagct gcgccgcggc
     9961 cgacccgagt gctccgtcga tggccaccag gccccgcaac accgattgcg agcgcgcgag
    10021 cgccgcggtg atgccgccat cgtcgccgcc gagcagggcc tcgacctgcg ccgtgccgtc
    10081 ggccaggcgg ccgaggctcg cgacgcgctt gcgctcgtcg gccagggcct ccgcttcgcc
    10141 cggtttcggg tcgagggccg cgaactcgcc cgcgtagtgc tgcagcaggt cgagccggct
    10201 cgtccggtcg cgcaggcgtt gctcgaacgt cgagcgacgc gtgtccagcg cgtgccaatc
    10261 gacgtaggca ctccgcacgg cctcgagccc ggcctccagt cgcccgctgc cgtcgagcgt
    10321 ctcgcgctgg tagccgcggc gcgcgagcgc ctggaactcg agctgcccgt ggacgtcgac
    10381 gaggaactcg ccgagcgcgc gcagggactg caccggcacg acctgcccgt tgagataggc
    10441 gcggccgcga ccgtccgcgg cgaccacgcg gcgcatctgg cactcgccgt cgtgcccgat
    10501 cgactgatcc tcgagccacg cgagcgcctg ggcgttgccg cgcacgtcga aggtcgcgct
    10561 gacctcggcg cgctccgcgc cgtgacgcac ggcgccgctg tcggcgcggc ccccgaccgc
    10621 gagcatcagc gcgtcgacca ggatcgactt gccggccccg gtctcgcccg tcagcacggt
    10681 gaagccgcgg gcgaattcga gctcgacggc ctcgacgatg gcgaagtcgc ggatctgcag
    10741 gtgcgtcagc atgggtcgtc aggcgcccgg tggctgcccc tcgcgcgtgc cgcggcccca
    10801 atgcagctta gagcgcagga tccgatagta gtcgtggccc ggcggatgca gcagcgtcgc
    10861 tgtcaccttg gcgcgctcga tgcgcaggcg cacgcccggt tcgaggtcgc agagcacgtg
    10921 accgtcgcag gcgacctgcg cgcgcgtgtc gaagcgatcg gcgagctcga tctcgatgag
    10981 gctgccggcc ggcacgacga tcggccggtc ggagagcgtg tgcggcgaga ccggtacgag
    11041 cacgatcgcg tcgaggctcg gatgcacgat cggaccgccg caggacagcg cgtaggccgt
    11101 cgagccggtc ggcgtcgcga cgatgaaacc gtcgccgcca tgcgtgttca cgtacactcc
    11161 gttgacccag gtgcgcacgt cgacggtgcg gccggtctcg cgcttggtca cgacgacgtc
    11221 gttgaggccg agcgagtcga tgggccgacc ctccgccatg aggtgcgccg cgagcagcat
    11281 gcggcgttcg gcgaggcaac gcccggcgag cgcgtcctcg acgctctcga tcatgcggtc
    11341 gggcccgacg tcggccagga agccgagtcg gccgcggttg acccccagca cggggacgtc
    11401 ggcgagcgcg gccatgcggg ccgcgtgcag catcgtgccg tcgccgccga ccgcgaccac
    11461 gaggtccgcg tccatcgcga ggtcgggctc gagcacggtg cgcacgccgt cgcgcgtcag
    11521 cacgagttcc tcggcggcgg ccaccttgac caggcagccc cgggcgagca ggtggctggc
    11581 cagcacgcgc atggcctcgc cgacgcgcgg gtcggccgca cggccggcca gggcgacgca
    11641 ggtgcggtcg gtggcgcgct gctccataaa gtggctgcgt tttagcacgt gcgcgctcgg
    11701 ccgggccagc gcggttcctg cacgggccgc ggtctttgct tgcttgactt gcccgggggc
    11761 gccgggcaac gttggccggg cccatgagcg acggccagga agaccaactc aacgaacgtg
    11821 cccggcacct gctgcgggtg ctggtcgaga gttacatccg cgaaggtcag ccggtcggct
    11881 cgcgcagcct gtcgcgcgac tccaagctca acctgagcgc ggcgaccgtc cgcaacgtga
    11941 tggccgacct cgaagagtac ggcttcgtca gctcgccgca cacgtccgcc gggcgcgtgc
    12001 ccaccgacaa gggttaccgg ttcttcgtcg acaccttgct gcgacagcgg cagatggcca
    12061 gcgtcgagaa cgtgatcgcc gagctgcagc agcggctcga gatgcaggag gaccgtgacc
    12121 ccaaggcact ggtcgccgtc gcctcacagg tgctgtcctc gatcacgcac ctggcaggcg
    12181 tggtgacgat cccgcgccag ccacaccagg ccctgagcca gatcgagttc ctgccgctgt
    12241 cggagaaccg cgtgctcgcg gtgatggtgg tcaacggccg ggacgtgcag aaccgcatcg
    12301 tgcacttgga acgctactac tcggccgagg aactgcggcg cgccgcgacg ttcctgaacc
    12361 agcagttcgc gggcaaggag ctgaaccagg tccgcaccga catcgtcacg gaactcacgc
    12421 agaccggcga gacgctcaac cagctgatgc gtgacgcgat cagggtcgcg cagcaggtgg
    12481 tcggcgaggg gagccggtcg ggcgaagccg agtatgtgat cgcgggcgag accaacctga
    12541 tggagttcgc ggagctctcg aacgtcgaga agctgcgccg gctgttcgac gcgttcacgg
    12601 ccaagcgcga catcctgcac ctgctcgacc agagcctgcg tgcagacggc gtgcagatct
    12661 tcatcgggca ggaatccggc taccagatcc tcgacgacat cagcctggtg accgccccat
    12721 acgcgctcga caacgagacg gtcggggtgc tcggcgtgat cggtccgacg cgcatggcct
    12781 acgaccgggt catcccgatc gtcgacctga cggcccgttt actgggctcc gccttgaatt
    12841 cccggcgtta gcccctagga agccgacaac gatccgtcca ggcctcccgc cacgtcgcgg
    12901 gcggccttcg ctttggcttg caggcaggtg acatgagcat caatccggaa catgaaccga
    12961 ttcccgcatc gtccgaggag actgcggacc agggtggagc gaccgaggtc gagcgcctcg
    13021 cgaccgagct ggaggcagtc cgggcccagc tgaccgaggc ccgggacgcc ca
//
LOCUS       DASPFR010000243         4031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1412584, whole genome shotgun sequence.
ACCESSION   DASPFR010000243 DASPFR010000000
VERSION     DASPFR010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412584"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VD737_06375"
     CDS             complement(<1..517)
                     /locus_tag="VD737_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXW10220.1"
                     /translation="MLGTTLLLAVREIRRHLLRSFLTILGIIIGVASVVTMVTLGNGA
                     TASVRAQLASLGSNVLTIRPGAGFGRGGGGPPAPPFDEADLEAIRGQVAGIVSVAPQN
                     QVGATVVRSGQNWSTTVLGTNNDFFQAAGIRLESGRTFSDGELKSGKAVCVIGQTIAS
                     NLFRDGDPVGKQ"
     gene            complement(517..1218)
                     /locus_tag="VD737_06380"
     CDS             complement(517..1218)
                     /locus_tag="VD737_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXW10221.1"
                     /translation="MNGPLIELRGVTKTYGSGPTAFEALKGIDLAVAPGDFVAVMGAS
                     GSGKSTAMNIVGCLDVPTSGSYRFKGVPVERLDQDQRALLRRRYLGFVFQGFNLLART
                     SALENVELPLLYRRDPSKIRHARAMRALEQVGLAEWHDHTPSELSGGQQQRVAIARAL
                     VTEPELLLADEPTGNLDTQRSIEIMELLSGLNRTRGITVIMVTHEPDIATYARTRVHF
                     RDGRVEGVDAPAETA"
     gene            complement(1215..2519)
                     /locus_tag="VD737_06385"
     CDS             complement(1215..2519)
                     /locus_tag="VD737_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXW10222.1"
                     /translation="MDSARPQTGLDEFLGTPPPGFLARRRRWVYGAAGLLVAIAAVVA
                     ISRGDGEGPAYATAPVTRGDLEVRISATGNLAPTNEVDVGSELSGLVEAVLVDVNDRV
                     TKGQPIARLDTLRLRDTIARSQAALQQAQAGVAQADATQRQAAAELARFEEVHRLSGG
                     KVPSATELDSARAEFDRAVATQRSARAQVASAQAQLSSDRTNLSKATIVSPVTGVVLS
                     RQVDPGQTVAASFNTPTLFTIAEDLSSMKLEVKVDEADVGQVADGQAATFTVDAFPDR
                     EFPAVIERVDVGANSGSTAAAQSSTSGGSAVVAYTAVLSVQNAELLLRPGMTATAEIV
                     THTEEGVLLVPNAALRFRPKTDAGPQRSALTMMPPRRERPQKTTRASRGSQQTIYVLG
                     PDGEPQPLAVTVGASDGTVTSVSGPGLQPGLEVITSELAPPT"
     gene            complement(2543..3961)
                     /locus_tag="VD737_06390"
     CDS             complement(2543..3961)
                     /locus_tag="VD737_06390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HXW10223.1"
                     /translation="MSLCSVLPPAWRTALAATVVVALAACAVGPDYRPPSAAQLSVPD
                     AYPSGARQPVDVAELRQWWTRFDDPVLAELVERSLDANLEIAAAEARLRTARASLTAA
                     RAAWWPALGASVDGSSRNVSDTPGGAVETWQAGFDAAWEVDVFGGTRRAAEAAAAGVG
                     EAGATLENVRSSIAAEVALNYIDARSAQQRLQVARESLASQDETVRITGWRHQAGLVS
                     AQDVEQATVLRAQTAAAVPQLRATFVAAANRIATLLGESPGGIAARLDAVAAAIPLGP
                     DAIGAGVPAELLRRRPDLIAAERAVAAEVARIGVAQADLYPALRLGGSLTSTTLAFGD
                     LGGDLVSAIGGSITVPLFQGGQLRARVEGQRAAADAALAAYESAVLRALEDVDNAMQA
                     IVAAHEREQQLEIAQAAAERSVALARTRYQSGLIDFQSLLDAERSLLSTRESRLNARA
                     ARAGASVQLYKALGGGWPSPAA"
BASE COUNT          545 a         1466 c         1448 g          572 t
ORIGIN      
        1 actgcttgcc cacggggtcg ccgtcgcgga acaggttcga ggcgatggtc tggccgatca
       61 cgcacacggc cttgcccgac ttgagttcgc cgtcggagaa cgtgcgtccg gactccagcc
      121 ggatgccggc ggcctggaag aagtcgttgt tcgtgccgag caccgtcgtc gaccagttct
      181 ggccgctgcg cacgaccgtg gcgccgacct ggttctgggg cgcgacgctc acgatgccgg
      241 cgacctggcc gcggatcgcc tcgaggtcgg cctcgtcgaa cggcggtgcc ggcggaccgc
      301 cgccgccgcg tccgaagccg gcccccggcc ggatcgtgag cacgttcgag ccgaggctcg
      361 ccagttgcgc gcgcaccgac gccgtcgcgc cgttgccgag cgtgaccatc gtgacgaccg
      421 aggcgacgcc gatgatgatg ccgaggatcg tcaggaacga ccgcagcagg tggcgccgga
      481 tctcgcgcac ggcgagcagc aacgtcgtgc cgagcatcag gctgtctccg ccggtgcgtc
      541 gacgccctcg acccgtccgt cgcggaaatg cacgcgcgtg cgcgcatacg tcgcgatgtc
      601 cggctcgtgc gtcaccatga tcaccgtgat gcctcgcgtg cggttgaggc cgctcagcag
      661 ctccatgatc tcgatcgagc gctgggtgtc gaggttgccg gtcggctcgt cggcgagcag
      721 cagctcgggc tccgtcacga gggcccgcgc gatcgcgacg cgctgctgct ggcctcccga
      781 cagctcggac ggggtgtgat cgtgccactc ggcgagaccg acctgctcga gcgcgcgcat
      841 cgcacgggcg tgccggatct tcgacggatc gcgtcggtac agcagcggca actcgacgtt
      901 ctccagcgcc gaggtgcgcg cgaggaggtt gaagccctgg aagacgaagc cgaggtagcg
      961 gcgccgcagc agcgcgcgct ggtcctggtc gagacgctcg accggcacgc ccttgaagcg
     1021 gtagctgccg gaggtcggga cgtcgagaca gccgacgatg ttcatcgcgg tcgacttgcc
     1081 ggagccggac gcgcccatca ccgcgacgaa gtcgcctggc gcgacggcga gatcgatgcc
     1141 cttgagcgcc tcgaacgcgg tcgggccgga accgtacgtc ttggtgacgc cgcgcagctc
     1201 gatcagcgga ccgttcatgt cggcggcgcc agctcgctcg tgatcacctc gagcccgggc
     1261 tgcaacccgg gcccggatac gctcgtcacc gtgccgtcgc tcgcgccgac cgtcaccgcg
     1321 agcggctgcg gctcgccgtc ggggccgagc acgtagatcg tctgctgcga gccgcggctg
     1381 gcgcgcgtgg tcttctgcgg acgttcgcgc cgcggcggca tcatcgtgag cgcgctgcgc
     1441 tgcggccccg cgtcggtctt cggccggaag cgcagggccg cgttcggcac cagcaggacg
     1501 ccttcctccg tgtgcgtgac gatctcggcg gtcgcggtca tgcccgggcg cagcagcagc
     1561 tcggcgttct gcaccgacag caccgcggtg tacgccacga cggccgagcc gccggacgtc
     1621 gacgattgcg cggccgccgt gctgccggag ttggcgccca cgtccacgcg ctcgatcacg
     1681 gcggggaact cccgatcggg aaacgcgtcg accgtgaacg tggccgcctg cccgtcggcc
     1741 acctgcccca cgtcggcctc gtcgaccttg acctcgagct tcatcgacga gaggtcctcg
     1801 gcgatcgtga acagcgtggg cgtgttgaac gacgcggcca ccgtctgtcc cgggtcgacc
     1861 tggcgcgaca gcacgacgcc ggtcaccggc gaaacgatcg tcgccttcga gaggttggtg
     1921 cggtcggacg acagctgcgc ttgcgccgag gccacttgtg cgcgcgcgga cctctgggtg
     1981 gcgaccgcgc ggtcgaactc ggcgcgagcg gaatcgagct ccgtggccga cggcaccttg
     2041 ccaccggaca ggcgatgcac ttcctcgaac ctcgcgagct ccgccgcggc ctgccgctgg
     2101 gtcgcgtccg cctgcgcgac gccggcctgc gcctgctgca gcgccgcctg gctgcgcgcg
     2161 atcgtatcgc gcagccgcag cgtgtccagt cgcgcgatcg gctggccctt cgtcacgcgg
     2221 tcgttgacgt ccaccagcac ggcttcgacg aggcccgaca actcggaacc gacatccact
     2281 tcgttggtcg gcgcgaggtt gccggtggcg gagatgcgaa cctcgaggtc gccgcgcgtc
     2341 accggcgcgg tggcataggc ggggccttcg ccgtcgccgc gagagatcgc cacgacggcg
     2401 gcgatcgcga ccagcagccc ggcggcgccg tacacccagc gtcgccgccg cgcgagaaag
     2461 cctggcggcg gcgtgcccag gaactcgtcg agtccggtct gcggtcgcgc tgaatccatc
     2521 gtgtcctcgc gggcgccgtc gatcatgcgg caggcgacgg ccaaccgccg ccgagcgcct
     2581 tgtagagctg caccgacgcg cctgccctcg cggcgcgcgc gttgagacgc gactcccggg
     2641 tcgagagcag gctgcgttct gcatcgagca gcgactggaa gtcgatcagt cccgactggt
     2701 accgcgtacg cgccagcgcg accgatcgct cggccgccgc ctgggcgatc tcgagctgct
     2761 gctcgcgctc atgcgcggcg acgatcgcct gcatcgcgtt gtcgacgtcc tcgagcgccc
     2821 gcagcacggc gctttcgtag gcggcgagcg ccgcgtcggc ggcggcgcgc tgcccctcca
     2881 cgcgggctcg cagctggccg ccctggaaca acggcaccgt gatcgagcca ccgatcgcgc
     2941 tcacgaggtc ccctcccagg tcgccgaacg ccagcgtcgt gctggtcaac gatccgccga
     3001 gccgcagggc cgggtagaga tcggcctgcg ccacgccgat gcgcgcgact tcggccgcga
     3061 ccgcacgctc ggccgcaatc aggtcggggc ggcgccgcag cagttcggcg ggcacgcccg
     3121 cgccgatggc gtcgggtccg agcgggatcg cggccgcgac cgcatcgagc cgcgcggcga
     3181 tgccgcctgg tgactcgccg agcagcgtcg cgatgcggtt ggccgccgcg acgaacgtgg
     3241 cccgcaattg cggaaccgcc gccgcggttt gcgcacgcag cacggtcgcc tgctcgacgt
     3301 cctgcgcact cacgaggccg gcctggtgtc gccagccggt gatccggacc gtttcgtcct
     3361 gcgacgcgag gctttcgcgc gcgacctgca ggcgctgctg tgcgctgcgc gcgtcgatgt
     3421 agttcagcgc gacctccgcg gcgatcgagc tccggacgtt ctcgagcgtc gcgccggcct
     3481 cgcccacgcc cgcggccgcc gcctcggcgg cacgccgcgt gcccccgaag acgtcgactt
     3541 cccacgccgc atcgaacccg gcctgccagg tctccacggc accgccgggc gtgtcggaga
     3601 cgttgcgcga ggaaccgtcg accgaggcgc cgagcgccgg ccaccaggcc gcgcgcgccg
     3661 ccgtcagcga ggcgcgcgcc gtgcgcagcc gcgcctcggc cgcggcgatc tcgaggttgg
     3721 cgtcgagcga gcgctcgacg agttccgcca gcacgggatc gtcgaagcgg gtccaccact
     3781 gccgcagttc cgccacgtcg accggctgcc gcgcccccga gggataggcg tcgggcacgg
     3841 acaactgggc ggccgacggc ggccgatagt cgggccccac ggcgcaggcc gccagcgcga
     3901 cgaccacggt ggcggcgagc gcggtgcgcc acgcgggtgg aaggacggaa cagagactca
     3961 tgctgcgcgt tatagggcgt ccgtcctacg cagggatttc aattctgaaa tgtggcggta
     4021 tgctcattgt g
//
LOCUS       DASPFR010000244         6007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_437187, whole genome shotgun sequence.
ACCESSION   DASPFR010000244 DASPFR010000000
VERSION     DASPFR010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437187"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..2224
                     /locus_tag="VD737_06395"
     CDS             83..2224
                     /locus_tag="VD737_06395"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXW10224.1"
                     /translation="MSTHPTTTRRDFLKGSAAATGALVIGFTLPAATRFADAATTAAG
                     TGSPFAPNAFLRIAANGEVTVVSGFSEMGQGVHTGIASLVAEELDADWKNVRLEQAPV
                     DKAYINPVFGMQATGGSSSIRGSYLPMRKAGAAARQMLVSAAAARWGVEAAACRTENG
                     RVLAADGRSLAYGELVEAASKLPVPQDPPLKDPKDFRIVGKGVANRLDTSRKVDGTAQ
                     FGLDVKLPGLLTAVMAHAPVPGAKVVSVDDTAAKAVPGVRKVVQIPQGVAVLADGYWP
                     ALKGRDALVVKWDDGPLGGLSSAAISKTLLEATTKPGSPARSEGDTNVPVAKTVDAIY
                     EAPYLAHACLEPMNCTAWVRAGDAEIWAPTQGPGPQQGIVAQLTGLAPEKVQIHTTYL
                     GGGFGRRFAADFIIAATLVSKAAGAPVKLVYTREDDTRAWFYRPASVARLSAGLDQDG
                     KPVSFTARIAAPALMTAAGFVTELPKGIDDPAVEGISDCPYDFPNLRVEYVRTEPGVP
                     VWFWRSVGHSQNAYFLESFIDEVAAAAGKDPVEFRRAMLGKQPRLRAVLDLAAQKAGW
                     GSPLPDGVHRGVAVAASFGSFVAQVAEVSLEAGGKPRVHRIVAAVDCGTAVNPEIVRR
                     QIEGAIVYGLSAALYEQITFANGRVQQSNFDDYAMLRMNEMPKVEVHIVPSSEPPSGV
                     GEPGLPPATPAVVNAIFAATGKRVRKLPLQA"
     gene            2236..2574
                     /locus_tag="VD737_06400"
     CDS             2236..2574
                     /locus_tag="VD737_06400"
                     /inference="COORDINATES: protein motif:HMM:NF022459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HXW10225.1"
                     /translation="MPRAALVVACLTLLPGLAAADTFRCGSRIISEGDTIEKVLQYCG
                     EPAERERTWMRRQPRFEYAGQEIPFEGTEDVPVDLWTYDFGPSRLMRRVRFIAGKVDS
                     VETLEHGGSR"
     gene            2792..3088
                     /locus_tag="VD737_06405"
     CDS             2792..3088
                     /locus_tag="VD737_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10226.1"
                     /translation="MRKTVMVTLVSSVLLGAAAAHAGDYKMTKEQFTAADTDRDGSLT
                     LAEAQAGAPKLAEKFAKLDADSDGKLSAAEFDAHSKAGDKSMESDQPTTPPKAE"
     gene            complement(3173..4525)
                     /gene="gorA"
                     /locus_tag="VD737_06410"
     CDS             complement(3173..4525)
                     /gene="gorA"
                     /locus_tag="VD737_06410"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008568724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HXW10227.1"
                     /translation="MSRKHDYDLLVVGAGSGGVRAARVAAAHGARVAIVEEHRIGGTC
                     VIRGCVPKKLLVYGAHFAEDLVDARRFGWTVENPSFDWPTLRDNVLAEVSRLSGLYAD
                     TLANHEVEMISGRAVLTGPHSVRVDGKELRADKLLIATGARPHVPDLPGAEHGITSNE
                     AFHLDAIPKRIVIAGGGYIANEFAGIFHEFGAEVTLLNRSDRILRGYDEQIRDRLLQI
                     SLTKGIRFLFNTKMQTITRHADGHLTLALSGGASSVDCDALMFATGRVPNTDGLGLET
                     AGVELDDAGAVRVDDDNRSTTCESVYAVGDVTNRVQLTPVAIREGQAFADTVFGSKLT
                     RVDYGCIPSAVFSHPPLAGVGLTESQARNQLGTIRVYTSDFRPMKNVLANRNERALYK
                     LVVNEATDKVVGAHMIGPDAPEILQLAAVAVKAGLTKAEFDACVAIHPTMAEEMVLLK
                     "
     gene            complement(4611..5648)
                     /locus_tag="VD737_06415"
     CDS             complement(4611..5648)
                     /locus_tag="VD737_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXW10228.1"
                     /translation="MLYNRLGDTGLIVSRLAFGAMTFGKADGAYAAIFKVGQDLANEL
                     VARSLDRGVNFFNTADVYANGESEVMLGRALGLRRRDVAIATKAGMRTGEPLLSSGLS
                     RRYLLNSAEDSLRRLGTDYIDVYLVHRPDPHTPVEETVDALDLLVRQGKVRYVGFSNW
                     PAWQAAKAIGLQQARGAEPFRAAEMYYSLVGRDLEHEVVPFARDAGVGIMVWSPLAGG
                     FLSGKYTREDPTGGGGRLTGFNFIPFDRERGYDLVDVLREIAKDREATPAQVALAWLL
                     HQAQVASVLVGASSTAQLDDNLAAADVTLSPDELERLDRLTAPAATYPGWFNRTIYDK
                     VAAEALAPKVT"
     gene            complement(5720..>6007)
                     /locus_tag="VD737_06420"
     CDS             complement(5720..>6007)
                     /locus_tag="VD737_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10229.1"
                     /translation="DCGVHMFGRIENQGHPFYGLDFVHVELSKDAGWAPPEFAAFVSS
                     VIEAGAARPDQMGAVRARLWELGLEPYDCLSPPLMDAIATHTAKTRGLLAA"
BASE COUNT          968 a         2042 c         2064 g          933 t
ORIGIN      
        1 tgtcgctgcg gcacgtacaa ccagatccgt gccgcgatca aggatgcggc cacggcgctc
       61 ggcgcctgac aggagaccgc caatgagcac ccatccgacc acgacgcgtc gcgacttcct
      121 gaagggctcg gccgccgcca ccggtgcgct ggtcatcggt tttacgctgc cagcggcgac
      181 acggttcgcc gatgcggcga ccacggcggc cggcacgggc agccctttcg cgccgaacgc
      241 gttcctgcgc atcgcggcca atggcgaggt cacggtggtg agcgggttct ccgagatggg
      301 ccagggcgtg cacacgggca tcgcctcgct ggtcgcagaa gagctcgacg ccgactggaa
      361 gaacgtgcgg ctggagcagg ctccggtcga caaggcgtac atcaatccgg tgttcggcat
      421 gcaggcgacc ggcggctcgt cgtcgatccg tggcagctac ctgcccatgc gcaaggccgg
      481 cgccgccgcg cggcagatgc tggtctcggc agcggccgcc cggtggggcg tcgaggcagc
      541 ggcctgcagg accgagaacg gccgtgtcct cgcggccgac ggtcgatcgc tggcctatgg
      601 cgaactcgtc gaggccgcgt cgaagctgcc ggtgcctcag gatcccccgc tcaaggatcc
      661 gaaggacttc cggatcgtcg gcaagggcgt cgcgaaccgt ctcgacactt cgcgcaaggt
      721 cgacggcacg gcgcagttcg gcctcgacgt gaaactgccg ggcctgctca cggccgtcat
      781 ggcacacgcg ccggtgccgg gcgccaaggt cgtgtcggtg gacgacaccg ccgcgaaggc
      841 ggtcccgggc gtccgcaagg tcgtgcagat tccgcaaggc gtcgcggtgc tcgccgacgg
      901 ctactggccg gcactcaagg gacgcgacgc gctggtcgtc aagtgggacg acggtccgct
      961 gggcggcctg tcgtcggctg cgatctcgaa aaccctgctc gaggcgacga cgaagccggg
     1021 cagcccggct cgcagcgagg gcgacacgaa cgtgccagtc gccaagacgg tcgacgcgat
     1081 ctacgaagcg ccctacctcg cgcacgcctg tctcgagccc atgaactgca cggcgtgggt
     1141 gcgcgccggt gatgccgaga tctgggcacc gacccagggc cccgggccgc aacagggcat
     1201 cgtcgcgcag ttgacgggac tggcgccgga aaaggtacag atccacacga cgtacctcgg
     1261 cggtggtttc gggcggcgtt tcgcggccga cttcatcatc gcggccacgc tggtttccaa
     1321 ggcggccggc gcgccggtca agctcgtgta cacgcgcgag gacgacacgc gtgcgtggtt
     1381 ctaccggccg gcgtcggtcg cccgcctgtc ggccggtctc gaccaggacg gcaagccggt
     1441 gtcgttcacg gcccgcatcg cggcgcccgc gctcatgacg gcggcgggct tcgtgacgga
     1501 actgccgaag ggcatcgacg atccggccgt cgagggcatc agcgactgcc cgtacgactt
     1561 cccgaacctg cgcgtcgagt acgtgcggac ggagcctggc gtgccggtgt ggttctggcg
     1621 ttcggtcggt cactcgcaga acgcgtactt cctcgagtcg ttcatcgacg aagtcgcagc
     1681 cgcggccggc aaggacccgg tcgagttccg ccgcgcgatg ctcggcaagc agccgcgcct
     1741 gcgcgccgtg ctcgacctgg ccgcgcagaa ggctggctgg ggatcaccgt tgccggacgg
     1801 cgtgcatcgg ggcgtcgcgg tcgccgcctc gttcggctcc ttcgtggcgc aggtcgcgga
     1861 agtgtcgctg gaggccggcg gcaagccgcg cgtgcatcgc atcgtggccg ccgtcgactg
     1921 cggcacggcc gtcaaccccg agatcgtgag gcgacagatc gagggcgcca tcgtctacgg
     1981 cttgagcgcc gcgctgtacg agcagatcac gttcgccaac ggccgcgtgc agcagtcgaa
     2041 cttcgatgac tacgcgatgc tgcgcatgaa cgagatgccg aaggtcgagg tgcacatcgt
     2101 gccgagttcg gagccgccga gcggcgttgg cgagccagga ttgccaccgg cgacgcccgc
     2161 cgtcgtcaat gcgatcttcg ccgcgacggg caagcgcgta cgaaagctgc cgttgcaggc
     2221 ctgagcgcac actgcatgcc gagagcggcc ctcgtcgtcg cctgccttac gctgctgccg
     2281 gggctcgccg cggcggacac gttccgctgt ggcagccgga tcatcagcga aggcgacacg
     2341 atcgagaagg tgctgcagta ctgcggtgaa ccggccgaac gcgagcgcac gtggatgcga
     2401 cggcagcctc gcttcgagta tgccggccag gagatcccct tcgagggcac ggaggacgtg
     2461 ccggtggacc tctggaccta cgacttcgga ccgagccggc tgatgcggcg ggtgcgcttc
     2521 atcgccggca aggtcgattc ggtcgaaacg ctggagcacg gcggatcgcg ctgacgctcc
     2581 gagtcagcac ttctcgcgaa cttccccacc cgcgctggag cgaggcgatc cgcgagatgc
     2641 ccggttctcg ccaggttcgc ttcgtcgatt gaggagctgc aaacctccgc gggccgcaag
     2701 gattggcggt tgtcgctaaa cgccgacgct tggcgccacg aaccctcgcg cacagcgaca
     2761 gtccaactcg gccctacaag gagaaaacat catgcgaaag acagtaatgg ttaccctggt
     2821 ttcgtccgta ctgctgggtg ccgcggcggc gcacgcaggc gactacaaga tgacgaagga
     2881 gcagttcacc gcggcggaca cggaccggga cggctcgctc acgcttgccg aggcccaggc
     2941 cggcgcgccc aagctggcgg agaagtttgc gaagttggac gccgacagcg acggcaagct
     3001 gtccgccgcc gagttcgacg cgcacagcaa ggcgggcgac aagtcgatgg agtctgacca
     3061 gccgacgacc ccgcccaagg ccgagtgacg atttgaatcc gacggtttga acccgtcgga
     3121 ttcgcaccag cgccggacgc atttccagtg cgtccggcgt tttttttgcg tgtcacttca
     3181 acagcaccat ctcctccgcc atcgtcgggt ggatcgcgac gcacgcgtcg aactccgcct
     3241 tggtgagccc ggccttcacg gcaaccgccg cgagttgcag gatctccggc gcgtcggggc
     3301 cgatcatgtg cgcgccgacg accttgtccg tagcttcatt gaccacgagc ttgtagagcg
     3361 cgcgctcgtt gcggttggcg agcacgttct tcatcgggcg gaagtcggac gtatagacgc
     3421 gaatcgtgcc cagctggttg cgcgcctgcg actcggtcag gccgacgccc gccagcggcg
     3481 ggtggctgaa caccgcggac gggatgcagc cgtaatcgac gcgcgtgagc ttgctgccga
     3541 acaccgtgtc ggcaaacgcc tgtccctcac gtatcgccac gggcgtgagc tgcacgcggt
     3601 tcgtgacgtc gcccacggcg tagacggatt cgcaggtagt cgaccggttg tcgtcgtcga
     3661 cgcgaaccgc accggcgtcg tccagttcga cgcccgccgt ctcgagtccg agaccgtcgg
     3721 tgttgggcac gcgcccggtc gcgaacatca acgcatcgca gtccacggaa ctcgcgccgc
     3781 cgctcagcgc cagcgtcagg tggccgtcgg cgtgcctggt gatggtctgc atcttcgtgt
     3841 tgaacaggaa ccggattccc ttggtcagtg agatctgcag cagccggtcg cggatctgct
     3901 cgtcgtagcc gcgcaggatc cggtccgagc ggttcagcaa cgtcacctcg gcaccgaact
     3961 catggaagat gccggcgaac tcattggcga tgtagccacc acccgcgatc acgatgcgct
     4021 tcgggatcgc atcgaggtga aacgcctcgt tggacgtgat gccgtgctcc gcgccgggca
     4081 ggtcgggaac gtgaggccgc gcaccggtcg cgatcaggag cttgtcggcg cgcagttcct
     4141 tcccgtcgac gcgcacggag tgcggccccg tcaggacggc ccggccgctg atcatctcga
     4201 cctcgtggtt ggcgagcgta tccgcgtaca ggccgctcag tcgcgagacc tccgccagca
     4261 cgttgtcgcg caacgtcggc cagtcgaagg agggattctc gacggtccag ccgaagcgcc
     4321 gcgcatcgac caggtcctcg gcgaagtgcg caccgtagac cagcagtttc ttgggcacgc
     4381 agccgcggat cacgcaggtg ccgccgatgc ggtgctcctc gacgatcgcc accctggcac
     4441 cgtgcgccgc ggcgacgcgc gccgcccgga cgccgcccga gcccgcaccg acgacgagca
     4501 ggtcgtagtc atgtttcctg gacacgccgg gactcctcga ggtcgtacga ggttacgagc
     4561 ttacccatcg ggcaggacga acgctcgcgg cgcggcccgc agctcgtctg tcacgtcacc
     4621 ttgggtgcca gggcttcggc agcgaccttg tcgtagatcg tgcggttgaa ccagccggga
     4681 tacgtcgcag ccggcgcggt cagccggtcc aaccgctcga gttcatccgg cgacagcgta
     4741 acgtccgccg cggccaggtt gtcgtcgagc tgcgctgtgc tgctcgcgcc gacgagcacc
     4801 gacgccacct gcgcctggtg cagcaaccac gcgagggcga cctgcgccgg cgtggcctcc
     4861 ctgtctttcg cgatctcccg cagtacgtcg acgaggtcgt agccgcgctc gcggtcgaaa
     4921 gggatgaagt tgaagccggt caaccgaccg ccacccccgg tgggatcctc tcgcgtgtac
     4981 ttgccggaca ggaagccacc ggcgagcggg ctccacacca tgatgcccac gcccgcgtcg
     5041 cgcgcgaagg gaacgacctc gtgctcgagg tcgcgcccga cgaggctgta gtacatctcg
     5101 gcggctcgaa acggctcggc gcccctggcc tgctgcagtc cgatggcctt ggcggcctgc
     5161 cacgcgggcc agttcgagaa cccgacatac cgcaccttgc cctgccgcac gagcaggtcg
     5221 agcgcatcga cggtctcttc gacgggcgtg tgcgggtcgg gccggtgcac gaggtagacg
     5281 tcgatgtagt cggtgccgag ccggcgcagg ctgtcttctg ccgagttcag cagataacgc
     5341 cgcgacaggc ctgagctcaa cagcggctcg cccgtgcgca ttccggcctt ggtggcgatc
     5401 gccacgtccc gccgccgtag cccgagcgca cgcccgagca tcacctccga ctcgccgttg
     5461 gcgtagacgt cggccgtgtt gaagaagttg acgccgcgat cgagcgatcg cgcgacgagc
     5521 tcgttcgcga ggtcctggcc gaccttgaag atggccgcgt acgcgccatc tgccttgccg
     5581 aaggtcatgg ccccgaaggc gaggcgcgac acgatcaggc ccgtgtcacc gagacggttg
     5641 tagagcatgc tcgtcgtttc tccgcatgta tcgatcgcca cggccgggcg caccacggga
     5701 taccggagcg cgaacgccct cacgccgcca gcaaaccccg ggtcttcgcc gtgtgtgtcg
     5761 cgatcgcgtc catcagcggc ggcgacaggc agtcgtacgg ctcgaggccg agttcccaga
     5821 gacgcgcgcg cacggcgccc atctggtccg gccgggcggc acccgcctcg atgacggaag
     5881 agacgaacgc cgcgaactcg ggcggtgccc agcccgcgtc cttcgacagc tcgacgtgga
     5941 cgaagtcgag gccgtagaac ggatggcctt ggttctcgat gcgtccgaac atgtgcacgc
     6001 cgcagtc
//
LOCUS       DASPFR010000245         2716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_924850, whole genome shotgun sequence.
ACCESSION   DASPFR010000245 DASPFR010000000
VERSION     DASPFR010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924850"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..669
                     /gene="lptE"
                     /locus_tag="VD737_06425"
     CDS             130..669
                     /gene="lptE"
                     /locus_tag="VD737_06425"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HXW10230.1"
                     /translation="MLASATLTGCGFHLQGAGQLPEDSRRVYISTSDELTPFAVELRR
                     AIERSGGTIASGSAAADLVVRIERDRSGRRVLSVSARNTPQEYEIYYNVEYTVDREGE
                     QVVERQPLELIRNLSFDQSQLLAKDREEAILRDAMARDLAMLVVRRLESLDETAPARD
                     SPGAERDGRAAPATDDVTR"
     gene            complement(803..1957)
                     /locus_tag="VD737_06430"
     CDS             complement(803..1957)
                     /locus_tag="VD737_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HXW10231.1"
                     /translation="MSSGSGRTLALVVAAAGLAVLLGACGGARSPGDGAADASMGPAE
                     EPVVHFANFAEEIGPETLAQFTRETGIEVVYDVYETNHALDAKLMAGRTGYDVVVPGS
                     NFLENQLKAQVYRPLDRAQLPNWKHLDPEILRRLERNDPGNHHSVPYLYGTHALGYNV
                     QLVRAALGRDPPDSWSLLFDPENARRLQRCGIVVPDTPWILVRHALLYLGRNPNGEDA
                     ADLADAMKALYAIRPYLRDITSALGIADYAEGQACVFAVPNADVRLARDQAAQAGTSV
                     ELRYVIPEEGGLLWFDMLAIPADAPHPGNAHKLIDFLLRPDVIAKVTEATYFANANRS
                     ADALVAPELRQDPLIYPDPAALERLYAVTAYSAQYARDQSREFTRFKTTR"
     gene            complement(1954..>2716)
                     /gene="pip"
                     /locus_tag="VD737_06435"
     CDS             complement(1954..>2716)
                     /gene="pip"
                     /locus_tag="VD737_06435"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HXW10232.1"
                     /translation="RIVLFDQRGCGRSRPHASLEDNTTWHLVADMEALRRHLGIERWL
                     VFGGSWGSTLSLAYAQSHPTRVTELVLRGIFMLRDWEIHWFYQHGADAIFPDRWEHYV
                     AAIPRAERDDLVQAFYKRLTSRNRATQLKAAKAWAVWEGSTSYLHVNDKNIENWNADD
                     FAVAVARIECHYFVNQGFFEREDQLLRGVRRIRDIPAVIVQGRYDVVCPMRSAWDLHR
                     AWPEAQLRIVPDAGHSALEPGNTHELVSATDRFAK"
BASE COUNT          413 a          925 c          942 g          436 t
ORIGIN      
        1 gtgaacccgt ccctgggggc tcgcactcgc catccatggc tcgtgacggt cttcccaggt
       61 cgttccggac ggaccagacc ggccgctccg atctccgcat cgcgggcagc gtccacgacc
      121 gcggcgctga tgctggcgag cgcgacgctc accggctgcg gtttccacct gcagggcgcc
      181 ggccaactgc ccgaggacag ccggcgcgtg tacatctcga cttcggatga gctcacgccg
      241 ttcgccgtcg aactgcgacg cgcgatcgag cgctccggcg gcacgatcgc gagcggctcc
      301 gcggccgccg acctcgtcgt gcgcatcgag cgcgatcgca gcgggcgacg cgtgctctcc
      361 gtctccgcgc gcaacacgcc gcaggaatac gagatctact acaacgtcga gtacaccgtc
      421 gaccgcgagg gcgagcaggt cgtcgagcgc cagccgctcg agctcatccg caacctgagc
      481 ttcgaccagt cccagctgct cgcgaaggac cgcgaggaag cgatcctgcg cgatgcgatg
      541 gcgcgcgacc tcgcgatgct ggtcgtgcgc cggctcgaat cactggacga gacggcgccg
      601 gcgcgcgatt cgcccggcgc ggagcgcgac ggccgcgccg cccccgccac cgacgacgtg
      661 acgcgatgaa gacgcgcctg ccggtcctcc tgtaccgcgt gctcgaggtc gccgtgatgc
      721 tggtgaccta cctcgtgctc ggcttcatcg tctacaaggc gatcacctgg ctcttctgag
      781 ccgagggtcc gtgccgaaaa cgtcagcgcg tcgtcttgaa gcgcgtgaac tcgcggctct
      841 ggtcccgcgc gtattgcgcc gaatacgcgg tgaccgcgta cagcctctcg agcgcggcgg
      901 ggtcggggta gattagcgga tcctggcgca actccggcgc gacgagtgca tccgcgcttc
      961 gattggcgtt cgcgaaatac gtcgcttcgg tcaccttggc gatcacgtcg ggacgcagca
     1021 ggaaatcgat cagcttgtgc gcgttgcccg ggtgtggcgc gtcggccgga atcgccagca
     1081 tgtcgaacca gagcagcccg ccttcctcgg ggatcacgta acggagttcg acgctcgttc
     1141 cggcctgcgc ggcctggtcg cgcgcgagcc gcacgtcggc attcggtacg gcgaacacgc
     1201 aggcctggcc ctcggcgtag tcggcgatac cgagggcgct cgtgatgtcg cgcaggtatg
     1261 ggcggatcgc gtacagcgcc ttcatcgcgt cggcgaggtc cgcggcgtcc tcgccgttgg
     1321 ggttgcggcc gaggtacagc agggcgtggc gtacgaggat ccagggcgtg tcggggacga
     1381 cgatcccgca ccgctgcagc cgacgcgcgt tctcgggatc gaacagcagg ctccacgagt
     1441 ccggcggatc gcgaccgagc gccgcgcgaa cgagttgcac gttgtagccg agcgcgtgcg
     1501 tgccgtagag atacggcacc gagtgatggt tgcccggatc gttgcgctcg agccggcgca
     1561 ggatctctgg gtcgaggtgc ttccagttcg gcagctgcgc gcgatcgagc ggccggtaca
     1621 cctgcgcctt cagctggttc tcgaggaagt tgctgccggg aacgacgacg tcgtagccgg
     1681 tgcggccggc catcagcttc gcatcgagcg cgtgattggt ctcgtagacg tcgtagacga
     1741 cttcgatacc ggtctcgcgg gtgaactggg cgagcgtctc gggaccgatc tcctcggcga
     1801 agttggcgaa gtggacgacc ggctcctcgg ccgggcccat ggacgcgtcc gctgcaccgt
     1861 caccaggcga tctggcgccg ccgcacgcgc cgagcagcac ggccagaccc gctgccgcga
     1921 cgaccaaggc gagcgtacgc ccggatccgg aactcacttc gcgaaccggt ccgtcgcgga
     1981 gaccagttcg tgcgtgttgc cgggttcgag cgcggagtgg ccggcgtcgg gcacgatgcg
     2041 cagctgcgct tcaggccagg cccggtgcaa gtcccaggcg ctgcgcatcg ggcagacgac
     2101 gtcgtagcgt ccctgcacga tcaccgccgg gatgtcgcgg atcctgcgca cgccacgcag
     2161 caactggtcc tcgcgctcga agaacccctg gttgacgaag tagtggcact cgatgcgggc
     2221 caccgccacc gcaaagtcgt cggcgttcca gttctcgatg ttcttgtcgt tgacgtgcag
     2281 gtagctggtc gagccctccc acacggccca cgccttggcg gccttcagct gcgtcgctcg
     2341 gttgcggctc gtgagccgct tgtagaacgc ctgcacgagg tcgtcgcgct ccgcgcgcgg
     2401 gatcgccgcg acgtagtgct cccagcggtc gggaaagatc gcatcggcgc cgtgctggta
     2461 gaaccagtgg atctcccagt cgcgcagcat gaagatcccg cgcagcacga gttcggtcac
     2521 gcgcgtcggg tgcgactggg cgtaggcgag cgacagcgtc gagccccacg acccgccgaa
     2581 cacgagccag cgctcgatcc ctaggtgcct gcgcagcgcc tccatgtcgg cgacgaggtg
     2641 ccaggtcgta ttgtcctcga ggctcgcatg cggccggctg cggccgcagc cacgctggtc
     2701 gaagagcacg atgcga
//
LOCUS       DASPFR010000246        16333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_192758, whole genome shotgun sequence.
ACCESSION   DASPFR010000246 DASPFR010000000
VERSION     DASPFR010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16333
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192758"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VD737_06440"
     CDS             <1..745
                     /locus_tag="VD737_06440"
                     /inference="COORDINATES: protein motif:HMM:NF042682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HXW10233.1"
                     /translation="LALGRLAAHDFAAVERLLADVQGPAVAVPATTLDAVVARRADVL
                     TEYQDRAYAERYVRLVHRVRDVERQRGLTGLADAVAQGYFKLLAYKDEYEVARLHSSP
                     AFLEQLERTFEGDYRLRFHLAPPLLTRPDSRTGQVRKHEYGAWMLPVFRLLARMKGLR
                     GTRLDPFGWLPDRKLERQLIADYERLIDECLARLRPDNHAVAVELASLPAQVRGYGHV
                     KRRHLEAVRARERELLAQLRSDAGVAQAA"
     gene            883..1719
                     /locus_tag="VD737_06445"
     CDS             883..1719
                     /locus_tag="VD737_06445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXW10234.1"
                     /translation="MQFPSIRFDGKVALVTGASSGIGRETALGFARAGAKVVLVARRS
                     AELAKVARIAREQHGATTLSVATDVTDAASVQACFRRAVTRFKRIDVVVNNAGVLIPA
                     PVARMDPADLQRMFDVNVFGALHVMQAAVRQMRRQADGGHIVNVGSLAGRRGYSPLGG
                     YSATKFALVGMTEALRTELADEPIGVSLVLPGVIDTPMATSAAANPVTAGFWPKKLTM
                     PPEWVVWAIFAAVRLRLAEVAVPPGGALLEKIAALTPGATDALVRGVVGLARRTGRTR
                     RG"
     gene            1764..2600
                     /locus_tag="VD737_06450"
     CDS             1764..2600
                     /locus_tag="VD737_06450"
                     /inference="COORDINATES: protein motif:HMM:NF004794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HXW10235.1"
                     /translation="MSEPFAHLAIERTGAVATLCLDRPDKLNALHRALWHSIPEAVAA
                     LERDPTVRVIVLAGKGKAFCAGIDLVDHAPGLGGGGSLSGVQGSPVAMRRQLYDDIRA
                     YQRTASCFADINKPVIAAVHGACLGAGVDLVTACDIRLAAADAVFSVRETRIAMVADV
                     GSLQRLPRVIGDGHAREWIFTGADYDAQRALEVGLLNAVLPDRDAAVARAMELAQAIA
                     ANSPLAVQGAKHVLGFASRREVDTQLDYTALWNAAFLHSEDLGEAMQAFVERRAPKFK
                     GG"
     gene            complement(2640..3143)
                     /locus_tag="VD737_06455"
     CDS             complement(2640..3143)
                     /locus_tag="VD737_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10236.1"
                     /translation="MKLLLLAFSVIHGLMAFMFAAAAVLLIIIAGKVGWVGFTGVLDR
                     SAAQQIIEAVGMLAAAVVALQISQTIAEEEVVRDAHISGPTRVRRFLSRFLVVVVVAL
                     TIEALVATFKSGNGQPQDLLYASSMVLAAGGLLAGWGLFIRTNRDAEELEPEAMAEAK
                     REDRKLK"
     gene            complement(3406..5115)
                     /locus_tag="VD737_06460"
     CDS             complement(3406..5115)
                     /locus_tag="VD737_06460"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HXW10237.1"
                     /translation="MERFWLKHYPPGVPHDVDVTQYASLVRLLEEGFQKHATRNAYAY
                     MDKFFTFADVDRHSAALGAWLQSRGLARGARVAIMMPNVVQYPIAVAGVLRAGYVVVN
                     VNPLYTPRELEHQLKDSGAEAIVILENFASTLQQVITKTAIRHVVLASLGDMLGFPKG
                     AIVNYTVRRVRKLVPEFSLPGHVRFTDALDAGARRRLEPVAVGPEDVAFLQYTGGTTG
                     VSKGATLTHRNLIANVLQMEAWIVPALHDTSRGPVPDQFVYICALPLYHVFALVVNCL
                     FGMRIGTLNVLITNPRDIDGFVKELAKYKFNVVPGVNTLYNALCENAEFRKLDFSSLR
                     VANGGGMAVQKAVSDKWKSITGLPIIEGYGLSETSPVATCNPIDNAEYNGTIGLPFPS
                     TEIAIIDDDGRPVPLGQPGEIAIRGPQVMAGYWNRPDETAKVMTPDGFFKSGDVGIMD
                     ERGYIRIVDRKKDMILVSGFNVYPNEIEAVLATHPGVIESAAVGVPDEKSGEAVKVFV
                     VRKDPSLTAEQLMEFCRQNFTAYKKPKYIEFRDELPKTNVGKILRRELRDAPMQAKQA
                     QTA"
     gene            complement(5162..6088)
                     /locus_tag="VD737_06465"
     CDS             complement(5162..6088)
                     /locus_tag="VD737_06465"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXW10238.1"
                     /translation="MKPPRARVAATMLASLAIAAAQAQGTPAAAPEASPGPRTMAEVL
                     AATTPADWRPLDPENTLYLQLPAGRVVIELAPAFAPRHADNLRTLVRQKYFDGLAVIR
                     SQDNFVVQWGDPDGKRALGQAQATIPPEFTVPLSPSLPFEPLPDRDGYAPEAGFSQGF
                     PAARDPARGEAWLAHCYGMVGAGRGNEPDSGNGTELYVVTGHAPRQLDRNIALVGRVV
                     QGMELLSVLPRGSGSLGFYERPEQHIPIISLRLAADVPAAERVPLEVLRTDTASFRAL
                     AEARRNRRDDWYLVPAGHVDVCNIPIVARTPP"
     gene            complement(6085..6312)
                     /locus_tag="VD737_06470"
     CDS             complement(6085..6312)
                     /locus_tag="VD737_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10239.1"
                     /translation="MPTGSNDVEFSEVRKLLHDVNDELSIAVLQLEMMLESGAVEAQS
                     RESLEESLDACRKAAESLKQVWMILGPRQPT"
     gene            complement(6325..7233)
                     /locus_tag="VD737_06475"
     CDS             complement(6325..7233)
                     /locus_tag="VD737_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HXW10240.1"
                     /translation="MEALAPARVLVVDDEVRALRLMSRVLASSAIACRTAESAAEALN
                     LLKVMPEIDVVVSDIYMPTTDGIEFLGTVRRDFADRPWLQLLLVTGQASLETAVAAMR
                     LEASDYLFKPIEPKTLREAVQHALTRAESIRTVRATTGDSPGARELQQIADTAKALAE
                     DMRRTIENDSGKRDRPAPGQPSPARPEDTSLRTLKLLQKLQEARSSIFGEAVMPEPAW
                     EMLAELMRARLAGQHLSVTSLALSSKSPMTTALRRIEDLIQGGLAARMPDPADRRRTY
                     VELTPEGMARMQLFLEGFARTALVSS"
     gene            complement(7379..7783)
                     /locus_tag="VD737_06480"
     CDS             complement(7379..7783)
                     /locus_tag="VD737_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXW10241.1"
                     /translation="MSADESPNGALILVVDDDDRSRELMRIVLRHAGYRVIAAPGPQA
                     AIALLATERPAVAIVDLLMPEMTGLDFCRWLRAQPALASMRFVLLTGMDTQDTRNEAR
                     EAGADTVVTKPFDRIRLLEQLDSLLAPRPTAP"
     gene            7987..8355
                     /locus_tag="VD737_06485"
     CDS             7987..8355
                     /locus_tag="VD737_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXW10242.1"
                     /translation="MRILMIEDNESNAYLARFLLERRGHQVVVATTGAEGVSLAHASR
                     FDLILLDLRLPDGDGCDFVPSLDPGAPGATPVVAVSAHALAADRVRAIEVGCRAFIEK
                     PIDVTTFADRIEELGGKTSV"
     gene            8525..10000
                     /locus_tag="VD737_06490"
     CDS             8525..10000
                     /locus_tag="VD737_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HXW10243.1"
                     /translation="MEAAQAVRDDGGEARATLEHTIDAREFEARPGQSACVLLDTGPL
                     ALLMRAVLADEACRRIDVQTYLYDDDETGIVLMDRLRAAAERGVQVRLLIDDNGLRTD
                     ECMPLLLDSLPNAEVRVFNPFRIRPRWTRPLQLPFEFRRLNHRMHNKLFVVDGVATIV
                     GGRNIAGHYFEADEDSNFTDIDVLLLGPVARDAEECFERYWSSDLAVPLKEALGKRDA
                     RMARERIERYVRAARFTRLKEMYRTQHCGLRDTLARGDRQVHWAHAKVLADDPRKASA
                     RRRRDAPQVMLDALKETWEQAREEVLAATAYFVPGRRGSGDLIRMRRRGVRVRILTNS
                     LASTDVPTVHAGYTRYRRALLRAGVELHEYQRFGNRPRRHHRASRCNALHAKTLVVDR
                     RLAWIGSFNLDPRSSRLNTEMAVLLESESLAADLAARMERDLAPDRSWRVMMRPGSRD
                     LRWLGERAGAPHEVPREPDASLWWRGWVKLARRFPYIERFL"
     gene            10003..10335
                     /locus_tag="VD737_06495"
     CDS             10003..10335
                     /locus_tag="VD737_06495"
                     /inference="COORDINATES: protein motif:HMM:NF020469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HXW10244.1"
                     /translation="MLVKFSTRHGQLVMLGEPAVALLRLGGHSGTVPSAVLATDLPAF
                     LERLRSGLDVHGDELSPAPPPPPDRDSRDDDAPRERPVSLRHRAVPLLDMVQTAIARD
                     SDLMWDRA"
     gene            10339..10713
                     /locus_tag="VD737_06500"
     CDS             10339..10713
                     /locus_tag="VD737_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10245.1"
                     /translation="MRAALLALPAFALLLLAAHFFHARAGVVAAVCILLITLLFVPRA
                     WAGRVVQVVLAVGAIEWVLTAYALAQVRMQHGDPYLRLVIILGSVALLTAVAAALFQH
                     PALRARFGFGGHPAGTPPAATD"
     gene            10809..12998
                     /locus_tag="VD737_06505"
     CDS             10809..12998
                     /locus_tag="VD737_06505"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HXW10246.1"
                     /translation="MNHLLVTAGALALLALPGAEAADTMPETKTAANNPLLAAWTGPY
                     GGVPPFDRAKVEHLEPALEAGMAQQLAEIDRIAKDPAAPTFENTIAALERSGRTLDRV
                     HTVYGVYSSTLNDEAVQAVEREMSPKLAAFSDQITQNAPLFERIAKVYESREKSGLTP
                     EQQRLTWLYYTNFVRAGAKLDADAKQRLSQINQELAGLYTQFSQNVLKDENEQAVTID
                     DEAGLAGLSAAIRDGLAQEAESRGQKGKWAVANTRSSVEPFLTYAENRALREKVWRMF
                     VMRGDNGGPTDNNATITRILQLRAERAKLLGFPTHAHWRLENSMAKTPERAMELMQAV
                     WPPAVARVHEEVADMQKIADAEQAGIRIEPWDYRYYAEKVRKAKYDLDENELTPYMQL
                     ENLREGMFYMAKQLFGFTFKQVEAAKVPVYHPDVRVWEVNDPQGKLVGLWYFDPYARK
                     GKRSGAWMNAYRTQERFDREVRTIVSNNSNFVKGKPGEPVLISWTDAEVLFHEFGHAL
                     HGLSSDVNYPSLAGTAVPRDYVEFPSQLLEHWLSTPEILDKYALHVETGKPIPRELVA
                     KIESADKFNKGFDTVEYLSSALVDMKLHLAGAQKIDPDKFERETLAALGMPSEIVMRH
                     RTPQFNHVFSGDGYSAGYYSYLWSDTITADAWEAFTEAGGAWDKQVGNRLKRLLALGN
                     TVDPADAYRAFRGRDPGIDALMRKRGFPVKDSSRASTSAAPEAKKTH"
     gene            complement(13062..15155)
                     /locus_tag="VD737_06510"
     CDS             complement(13062..15155)
                     /locus_tag="VD737_06510"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXW10247.1"
                     /translation="MADGAAKLAKTGPTPPVAAMRPHVVSSPHGTRVDDYYWLRDDTR
                     TSPEVLAYLKAENDYRDAVTAPMRPLEQELYREIVGRILQDDSSVPYRDHGWYYYRRF
                     ETGDEYPIFARRPGSLEAPEQVLVDLREQAKGLDYYDIGALEVSDDNRLLAYTEDTVG
                     RRQHTLRFKSLETGQLMPDAVPNVEEGMAWAADNRRVFYVEKDPVTLLGLRVRVHVLG
                     TDPAQDALVYEEQDESFYTSVQLSKDRRYVLVHSSSTITSELRYARTDDPELRFRVLL
                     PRERGHEYSADHLDGRWIIRTNWDARNFRIMEAPVDAPADRSHWREVVPHRDDVYIAG
                     FDVFRDFLALEERVDGLRKVRIRPWLGGREFHVESDEPAYRMALGANEEIDSHVVRYT
                     YTSLTTPYTTYDYDVRSGQRTLLKREPVLGDFDPSRYRTEHLWVPARDGERVPVALVY
                     RKDTFRCDGTAPLLQYGYGAYGISSDPVFSSTTLSLLDRGFVYAIAQVRGGQERGRRW
                     YEAGRQLQKKHSFEDFIDVTRYLVAERYADPRRVFSRGGSAGGLLVAAVANMAPELYR
                     GIVAHVPFVDVVTTMLDESIPLTTNEYDEWGNPAEQAYYDYMLSYSPYDNVAQQDYPA
                     MFVTTGLWDSQVQYFEPAKWVAKLRRCKTDRRLLVMRTNMEAGHGGKSGRFEHLREIA
                     EEYAFVISEALAPGA"
     gene            complement(15170..15997)
                     /locus_tag="VD737_06515"
     CDS             complement(15170..15997)
                     /locus_tag="VD737_06515"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXW10248.1"
                     /translation="MDFHKPLLAFSTSLIVAACATSPTGRKQLMLVSEQQAISASRQA
                     YAQEIGKYKQEGKLVTDPRLLNRIEQITERLVGQAVRMRPESAQWQWSVQVIDDPKTI
                     NAWCMAGGRMAIYTGLIQKIDPTDDELAQVMGHEIAHALANHTAERMSMALASNAGVL
                     AAGVLSDNPGQSMALAAAAAQLAVQLPNSRTSENEADRIGIELAAKAGYDPRAAITLW
                     QKMGKAGGGAPPEFLSTHPSDASRQDRLGKLVPKMLPAYQSSGSRPTHPVRMGPAAV"
BASE COUNT         2477 a         5641 c         5642 g         2573 t
ORIGIN      
        1 gctcgcactc gggcggttgg ccgcgcacga cttcgcggcg gtcgaacgac tgctcgccga
       61 cgtgcagggc cccgccgtcg ccgtcccggc gaccacgctc gacgcggtcg tcgcccgacg
      121 cgccgatgtc ctgacggagt accaggaccg cgcgtacgcg gaacggtacg tgcggctggt
      181 gcaccgggtg cgcgacgtcg aacgtcaacg cggcctcacc ggcctcgccg acgccgtcgc
      241 gcagggctac ttcaagctgc tcgcctacaa ggacgagtac gaggtcgcgc ggctgcacag
      301 ctcgccggcg ttcctcgagc aactcgagcg cacgttcgag ggcgattacc ggttgcgctt
      361 tcacctcgcc cctccgctgc tcacgcgccc cgattcgcgc acgggccagg tgcgcaagca
      421 cgagtacggc gcgtggatgc tgcccgtgtt ccggctgctc gcccgcatga aggggctgcg
      481 cggcacccgc ctcgatccgt tcgggtggct gcccgaccgc aagctcgaac ggcagctgat
      541 cgcggattac gagcggctga tcgacgaatg cctggcgcgg ctgcggcccg acaaccacgc
      601 cgtggccgtc gagctggcgt cgctgcccgc gcaggtgcgg ggctacggcc acgtcaagcg
      661 ccggcacctc gaagccgtgc gcgcgcgcga acgcgagttg ctcgcgcagt tgcgcagcga
      721 cgccggggtg gctcaggcgg cctgaccgga aattggggac agtccctttt ttccgtggaa
      781 aatggggaca ctcctgactt ccggcgacag cctgcagggc ccagcgacga cggcgagaaa
      841 atcaggagtg tccccatttt ccggacgcgc taggcttgca gcatgcagtt tccctcgatc
      901 aggttcgacg gcaaggtggc gctggttacc ggcgcctcga gcggcatcgg ccgcgaaacg
      961 gcgctcggct tcgcgcgcgc cggcgccaag gtcgtgctcg tcgcgcggcg ctcggcagaa
     1021 ctcgccaagg tcgcgaggat cgcgcgcgag cagcacggcg ccacgacgct ctcggtggcc
     1081 accgacgtca ccgacgccgc ctcggtgcag gcctgcttcc gccgggccgt gacgcgcttc
     1141 aagcgcatcg acgtcgtcgt caacaatgcg ggcgtgctga tccccgcgcc cgtcgcgcgc
     1201 atggacccgg ccgacctgca acgcatgttc gacgtcaacg tgttcggcgc gctgcacgtg
     1261 atgcaggcgg cggtgcggca gatgcgacgc caggccgacg gcggccacat cgtcaacgtg
     1321 ggcagcctgg cgggccggcg cgggtactcg ccgctcggcg gctattcggc gaccaagttt
     1381 gcgctggtcg gcatgaccga ggcgctgcgc accgagctcg ccgacgaacc gatcggcgtg
     1441 tcgctggtgc tgcctggcgt gatcgacacg ccgatggcca cctcggccgc ggccaatccc
     1501 gtgacggcag gcttctggcc gaagaaactg acgatgccgc ccgagtgggt cgtctgggcg
     1561 atcttcgccg cggtgcggct gcgcctggcc gaggtggctg taccgcccgg tggcgccctg
     1621 ctcgagaaga tcgcggcgct gacgccgggc gcgaccgatg cgctcgtgcg cggcgtcgtc
     1681 gggctggcgc gtcgcaccgg ccgcacgcgc cgcggctgaa accgggtcgc cgcggcttcg
     1741 atcaccaccc gaggttgccg agaatgtcag agccgttcgc ccacctggcc atcgagcgca
     1801 cgggcgccgt ggccacgctg tgcctcgacc gtcccgacaa gctgaacgcg ctgcaccgtg
     1861 cgctctggca ctccattccg gaggccgtcg cggcgctcga acgcgatccc acggtgcgtg
     1921 tcatcgtcct ggccggcaag ggcaaggcgt tctgcgccgg catcgacctc gtcgatcacg
     1981 ccccggggct gggcggcggc gggtcgctgt cgggcgtgca aggctcgccc gtggcgatgc
     2041 gccgccagct ctacgacgac atccgggcct accagcgcac cgcctcctgt ttcgccgaca
     2101 tcaacaagcc cgtgatcgca gcggtgcacg gcgcctgtct cggggccggc gtggacctgg
     2161 tgaccgcctg cgacatccgc ctcgcggcgg ccgacgcggt tttctccgtg cgcgagacgc
     2221 ggatcgccat ggtcgcggac gttggctcgc tgcagcgact gccgcgagtg atcggggacg
     2281 gccacgcgcg cgagtggatc ttcacgggcg ccgactacga cgcgcagcgc gcgctggagg
     2341 tcggcctgct caacgccgtg ctgccggacc gcgacgcggc agtggcgcgg gcgatggaac
     2401 tcgcgcaggc gattgcggcc aactcgccgc tcgcggtcca gggtgccaag cacgtgctgg
     2461 ggttcgcctc acgtcgcgag gtcgacaccc agctcgacta cacggcgctc tggaacgcgg
     2521 cgttcctgca ctccgaagac ctcggcgagg cgatgcaggc gttcgtggaa cgcagggcgc
     2581 cgaagttcaa gggcgggtga tcctgccacc ggcggccgac ggccccgcca cgtccgtggc
     2641 tacttgagct tccggtcttc gcgcttcgcc tccgccatgg cctccggctc gagttcctcg
     2701 gcgtcccggt tcgtgcgtat gaacaggccc caaccagcca gcaggcctcc ggcggccagc
     2761 accatgctcg aggcatacag caagtcctgg ggttgcccat ttccggactt gaaagtcgcg
     2821 accagggctt cgatcgtcag cgccacgacg accacgacca ggaacctcga aaggaaacgc
     2881 ctgacccgcg tcggcccgct gatgtgcgcg tcgcgcacga cttcttcctc ggcgatcgtc
     2941 tgcgagatct gcaacgcgac gacggcggcg gccagcatac cgacggcttc gatgatctgc
     3001 tgggccgcac tccggtcgag cacgcctgtg aaaccgaccc agccgacctt ccccgcaatg
     3061 atgatcagca ggaccgcggc ggcggcgaac atgaatgcca tcaggccgtg gatgaccgag
     3121 aacgccagca acaggagttt catcgtgtcc ccgtttcagt gtgtcgcatg accgccgggc
     3181 gtgcagcagc cgcactccgc atgttcgtcg ccgcggccgc cacggatgaa gcgcgaacgc
     3241 cgtgcacgtc acgtgacgtt cacgcatgag cccgacacgc gtccagcgtg tcgggcactg
     3301 tactcgggtc tggctgaaac ctcgatcctg gtcgcaaggc agcgttgcct cgctggcccc
     3361 tggacgggat ccggccgttc gtggtctacg tgggccgatc cagcctcatg ccgtctgtgc
     3421 ctgcttcgcc tgcatcggcg cgtcccgcag ctcgcgccgc aggatcttgc cgacgttggt
     3481 cttcggcagc tcgtcgcgga actcgatgta cttcggcttc ttgtacgccg tgaagttctg
     3541 cctgcagaac tccatgagct gctcggcggt gagcgacggg tccttgcgga cgacgaagac
     3601 ctttacggcc tcgccggact tctcgtcggg cacgccgacg gccgcggact cgatcacgcc
     3661 ggggtgcgtc gcgagcacgg cctcgatctc gttcgggtac acgttgaagc ccgacaccag
     3721 gatcatgtcc ttcttgcggt cgacgatgcg gatgtagccg cgctcgtcca tgatgccgac
     3781 gtcacccgac ttgaagaacc cgtccggcgt catgaccttg gccgtctcgt cgggccggtt
     3841 ccagtagccg gccatcacct gcggtccgcg gatggcgatc tcgccaggct ggccgagcgg
     3901 cacggggcgg ccgtcgtcgt cgatgatcgc gatctcggtg ctcgggaaag gcaggccgat
     3961 cgtgccgttg tactcggcgt tgtcgatcgg gttgcaggtc gcgaccggcg aggtctccga
     4021 cagtccgtag ccctcgatga tcggcagccc ggtgatcgac ttccacttgt cggagacggc
     4081 cttctgcacg gccatgccgc cgccgttcgc gacgcgcagg ctcgagaagt cgagcttgcg
     4141 gaactcggcg ttctcgcaca gcgcgttgta gagcgtgttg acgcccggga ccacgttgaa
     4201 cttgtacttg gcgagttcct tgacgaagcc gtcgatgtcg cgcgggttcg taatcaacac
     4261 gttgagcgtg ccgatgcgca tgccgaacag gcagttcacg accagcgcga agacgtggta
     4321 cagcggcagc gcgcagatgt agacgaactg gtcgggcacc ggcccgcgcg aggtgtcgtg
     4381 gagcgccggg acgatccacg cctccatctg cagcacgttc gcgatcaggt tgcggtgcgt
     4441 gagcgtcgcg cccttggaaa cgccggtcgt gccgcccgtg tactgcagga aggcgacgtc
     4501 ttcggggccc accgcgacgg gctcgagcct gcgtcgcgca ccggcatcga gcgcatcggt
     4561 gaagcgcacg tgaccgggca gcgagaactc cggcaccagc ttgcgcaccc ggcgcacggt
     4621 gtagttgacg atggcgccct tggggaaccc cagcatgtcg ccgagcgagg ccagcacgac
     4681 gtgcctgatg gccgtcttcg tgatgacctg ctgcagcgtc gacgcgaagt tctcgagtat
     4741 cacgattgcc tcggcgcccg aatccttgag ctggtgctcg agctcgcgtg gcgtgtagag
     4801 cgggttcacg ttgaccacga cgtagccggc gcgcagcacg ccggcgacgg cgatcgggta
     4861 ctgcacgacg ttcggcatca tgatcgccac gcgcgcgccg cgcgcgaggc cgcgcgactg
     4921 cagccacgcg ccgagcgccg cggaatggcg gtccacgtcg gcgaacgtga agaacttgtc
     4981 catgtacgcg tacgcgttgc gcgtcgcgtg cttctggaag ccttcctcga gcagccggac
     5041 gagcgaggca tactgcgtga cgtccacgtc gtgcggtacg cccggtggat agtgcttgag
     5101 ccagaaacgt tccatggtcg ctccccctat tgtgcggtct gctcgcgcgg cgcctcgtcg
     5161 ctcagggcgg cgtgcgcgcg acgatcggta tgttgcagac gtcgacgtgg cccgccggga
     5221 cgagatacca gtcgtcccgc cggttgcgcc tcgcctcggc gagggcgcga aaacttgcgg
     5281 tgtccgtgcg caggacttcg agcggcacgc gttcggccgc cggcacgtcg gccgccaggc
     5341 gcaggctgat gatcggaatg tgctgctccg gccgctcgta gaacccgagc gagcccgatc
     5401 cgcgcggcag caccgacagc agctccatgc cctgcacgac gcgaccgacg agggcgatgt
     5461 tgcggtcgag ttgccgcggc gcgtggccgg tgacgacgta gagctcggtg ccgttgccac
     5521 tgtccggctc gttgccgcgc ccggccccga ccatgccata gcagtgcgcg agccatgcct
     5581 cgccgcgagc cgggtcacga gcggccggaa agccttggct gaaaccggct tcgggcgcat
     5641 agccatcgcg gtcgggcagc ggttcgaacg gcagcgacgg gctcagcggc accgtgaatt
     5701 cgggcgggat cgtagcctgc gcctggccga gcgcgcgctt gccgtccggg tcgccccact
     5761 gcacgacgaa attgtcctgc gagcggatga ccgccagccc gtcgaagtac ttctgtcgca
     5821 cgagtgtgcg caggttgtcc gcgtgccgcg gcgcaaacgc aggggcgagc tcgatcacga
     5881 cgcggccagc cggcaactgc aggtacagcg tgttctcggg gtcgagcggc cgccagtcgg
     5941 cgggggtcgt cgcggcgagg acttcggcca tggtgcgagg cccgggcgac gcttccggcg
     6001 ccgcggccgg cgtaccctgc gcctgcgcgg ccgcgatggc gagcgacgcc agcatcgtgg
     6061 cagcgacgcg ggcgcgcggc ggtttcacgt cggctgcctc gggccgagga tcatccacac
     6121 ctgcttcagg ctctccgccg ccttgcgaca ggcatcgagc gactcctcca gcgattcgcg
     6181 cgactgcgcc tcgaccgcgc cggattcgag catcatctcg agctgcagca ccgcgatcga
     6241 caattcgtcg ttgacgtcgt gcaggagctt gcggacttct gagaactcga catcgttgct
     6301 gcctgtgggc atgcgggcgc ttcctcaact cgaaaccagg gccgtgcgcg caaacccttc
     6361 caggaacagc tgcatccgtg ccatgccttc gggcgtgagt tcgacgtacg tgcgccggcg
     6421 gtccgccggg tcgggcatcc gggcagcgag tccgccctgg atcaagtcct cgatgcgacg
     6481 cagcgcggtc gtcatcgggc tcttcgacga cagcgcgagg ctcgtcaccg acaggtgctg
     6541 acccgccagg cgcgcgcgca tcagttccgc gagcatctcc caggcgggct ccggcatcac
     6601 ggcctcgccg aagatgctcg agcgcgcttc ctgcagcttc tgcaacagct tgagcgtgcg
     6661 caggctcgtg tcctcgggac gcgccgggct cggctggccc ggcgcaggcc ggtcgcgctt
     6721 gcccgagtcg ttctcgatcg tgcgtcgcat gtcctcggcg agtgccttgg cggtgtcggc
     6781 gatctgctgc agttcgcgcg cgcccggcga gtcacccgtg gtcgcgcgga ccgtgcggat
     6841 gctctcggcg cgcgtcagcg cgtgctgcac ggcctcgcgc agcgtcttgg gctcgatcgg
     6901 cttgaacagg tagtcgctcg cctcgagccg catcgcagcg accgccgtct ccagcgacgc
     6961 ctgcccggtg accagcagca actgcagcca gggccggtcc gcgaagtcgc gacgcacggt
     7021 tccgaggaac tcgatgccat cggtcgtggg catgtagatg tcgctgacga cgacgtcgat
     7081 ctcgggcatg accttgagga ggttgagcgc ctccgcggcc gattccgcgg tccggcaggc
     7141 gatggcgctg ctggcgagca cgcgcgacat cagccgcagc gcgcgcacct cgtcgtcgac
     7201 caccagcact cgggcgggag ccagggcttc catgtcgaaa atctacaacg gagcgccggc
     7261 aggcgacagt ccgcactggg cgcagtcact tcgaattagc cgagggtcgg cggcgcgacg
     7321 gcggcttgcc tggcgttgcg aacgtgcgtc cgtgccgttc atgggagggt ctcctgggtc
     7381 agggcgccgt gggccggggt gcgagcaggg aatcgagctg ttcgagcagc cggatccggt
     7441 cgaagggctt ggtgacgacg gtgtccgcac ccgcctcgcg cgcttcgttc cgggtgtcct
     7501 gggtgtccat gcccgtcagc agcacgaacc gcatcgacgc cagcgccggc tgcgcgcgca
     7561 gccaccgaca gaaatcgagc ccggtcatct ccggcatcag caggtcgacg atggccacgg
     7621 ccggccgctc ggtcgcgagc agggcgatcg ccgcctgcgg cccgggggcg gcgatcacgc
     7681 ggtagccggc gtgccgcagc acgatgcgca tcagctcgcg cgaacggtcg tcgtcgtcga
     7741 cgaccaggat cagggcaccg ttcggggatt cgtctgccga catggcgggg gattctaacg
     7801 gcactcgccg cgcgtggcag cgcagtgtgg actgcaaaga ttgccgcggt gccacgaacg
     7861 cacgaaatat tcttgcactc gaggaccggc agacttgcgc tgtgcaccgc cgctagaatc
     7921 gggcaggcgc gttccggccg agtgtgctgc cgcccaggac gtcgccatcg ggggagacac
     7981 gagccagtgc gcatattgat gatcgaggac aacgaatcga acgcctacct ggcgcgattc
     8041 ctgctcgagc ggcgcggcca tcaagtcgtc gtggcgacga ccggcgccga aggagtctcg
     8101 ctcgcccacg cctcccgctt cgacctgatc ctgctcgacc tccgcctgcc ggacggcgac
     8161 ggttgcgact tcgtgccgag cctcgatccc ggcgcgcccg gcgccacacc cgtcgtcgcc
     8221 gtgagcgctc acgcgctggc cgccgaccgc gtccgcgcca tcgaggtcgg ctgccgggcc
     8281 ttcatcgaga aacccatcga cgtcacgacc ttcgcggacc ggatcgaaga gctgggcgga
     8341 aagaccagtg tctagtgtgt agtgttcagt aagaggcggt gcgtggcgcc gatgtcgcgc
     8401 ccattgaagt tgccgcccta ctaaccgcta gacactggac actagacact cctctccgct
     8461 gtcagcgtcc gcccactgcc cgttgcggtc gtgcgcgggg cggcggcggg ctagcatttt
     8521 gaccatggaa gcggcgcagg cagtcaggga cgatggcggc gaggcccgcg cgacgctcga
     8581 gcacacgatc gatgcgcgcg agttcgaggc acgccccggc cagtccgcct gcgtgctcct
     8641 cgacacgggc ccgctcgcac tgctgatgcg tgccgtgctc gccgacgagg cctgtcgccg
     8701 catcgacgtg cagacctacc tctacgacga cgacgaaacc ggcatcgtgt tgatggaccg
     8761 gctgcgggcg gcggccgagc gcggggtgca ggtccggctg ctgatcgacg acaacgggct
     8821 gcgcaccgac gagtgcatgc cgctgctgct cgattcgctg ccgaacgccg aggtccgcgt
     8881 cttcaatccg ttccgcatcc ggccgcgctg gacgcgaccg ctgcaactgc cgttcgagtt
     8941 ccgccggctc aaccaccgca tgcacaacaa gctgttcgtg gtggacggcg tcgcgaccat
     9001 cgtgggcgga cgcaacatcg ccgggcacta tttcgaggcc gacgaggact cgaacttcac
     9061 cgacatcgac gtgctgctgc tcggtcccgt ggcgcgcgac gccgaggagt gtttcgagcg
     9121 ctactggtcg agcgacctgg cggtgcccct gaaggaggcc ctcggcaaac gcgacgcccg
     9181 catggcccgc gagcgcatcg agcgctacgt gcgggcggcg cgcttcacgc gcctgaagga
     9241 aatgtatcgc acgcagcact gcgggctgcg tgacacgctc gcccgcggcg accgacaggt
     9301 gcactgggcg cacgcgaaag tgctggcgga cgacccgcgc aaggcgagcg cgcgccgtcg
     9361 gcgggacgcg ccgcaggtca tgctggacgc gttgaaagag acctgggagc aagcgcgcga
     9421 ggaagtgctg gcggccacgg cgtacttcgt gccggggcgc aggggctcgg gcgacctgat
     9481 ccggatgaga cggcgcggcg tacgcgtgcg gatcctcacc aactcgctcg cgtcgaccga
     9541 cgtgcccacg gtgcacgctg gatacacgcg gtaccggcgt gcgctgctgc gtgccggggt
     9601 agagctgcac gagtaccagc gcttcggcaa tcgccccagg cgccatcacc gcgcgtcgcg
     9661 ctgcaacgcg ctgcacgcca agacgctggt cgtcgatcgc aggctcgcgt ggatcggcag
     9721 cttcaacctc gacccgcgct cctcgcggct caataccgag atggccgtcc tgctcgaatc
     9781 cgaatcgctg gccgcggacc tcgccgcccg catggagcgc gacctcgcgc cggaccgcag
     9841 ctggcgggtt atgatgcggc ccggcagccg cgacctgcgc tggctcggtg aacgagccgg
     9901 cgcaccccac gaggttcccc gcgaacccga cgcgtcgctc tggtggcgcg ggtgggtcaa
     9961 gctcgcgcgc cggtttcctt acatcgagag gttcctctga cgatgctggt gaagttctcg
    10021 acacgccacg gccagctcgt gatgctcggc gaacccgcgg tggcgctgct ccgcctcggt
    10081 gggcactccg gcaccgtacc gagcgcggtg ctcgccaccg acctgcccgc cttcctcgag
    10141 cgcctgcgca gcggcctcga cgtgcatggc gacgagctgt cacccgcccc gccgccgccg
    10201 cccgaccgtg actcacgaga cgacgatgcg ccacgcgagc gccccgtgag cctgcgccac
    10261 cgcgcggtgc ccctgctcga catggtgcag acggccatcg cgcgcgactc cgacctcatg
    10321 tgggacaggg cctgagccgt gcgggccgcc ctgctcgctc tgccggcgtt cgcgctgctg
    10381 ctgcttgcgg cgcacttctt ccatgcccgg gccggcgtcg tggccgcggt ctgcatactg
    10441 ctgatcacgc tgttgttcgt cccgcgcgcg tgggccggcc gggtagtgca ggtcgtactc
    10501 gcggtgggcg cgatcgagtg ggtcctgacc gcatatgcgc tcgcgcaggt gcgcatgcag
    10561 cacggtgacc cctacctgcg tctcgtgatc atcctcggca gcgtggcgct gctgactgcg
    10621 gtcgcggcgg cgctgttcca gcacccggcc ctgcgggccc ggttcggatt cggcggccat
    10681 ccggccggga cgcccccggc cgcgacggac tgacctccgg ctgcgacggg ccggtgggct
    10741 cgtcgcgcgt cgtgcggtaa attaggcggt ctccgtgccc ccgggcacga tcccgtccaa
    10801 ggacacgcat gaatcacctg ctcgtcacgg cgggcgcgct cgcgctgctc gccctgcccg
    10861 gcgcggaagc cgccgacacc atgcccgaga ccaagaccgc cgccaacaat ccgctgctcg
    10921 cagcctggac cggcccgtac gggggcgtgc cgccgttcga ccgcgcgaag gtcgagcacc
    10981 tcgagcccgc cctcgaggcg ggcatggcgc agcagctcgc cgagatcgac cgcatcgcga
    11041 aggaccccgc ggcgccgacg ttcgagaaca cgatcgccgc gctcgagcgc agcggccgca
    11101 cgctggaccg cgtgcacacc gtctacggcg tctacagctc gacgctcaac gacgaggccg
    11161 tgcaggcggt ggagcgcgag atgtcgccga agcttgccgc gttcagcgac cagatcacgc
    11221 agaacgcgcc gctgttcgaa cggatcgcga aggtctacga gtcccgcgag aagtcgggcc
    11281 tcacgccgga gcagcagcgg ctcacctggc tctactacac gaacttcgtg cgcgccggtg
    11341 cgaagctcga tgccgacgcg aagcagcgcc tgtcgcagat caaccaggag ctcgccgggc
    11401 tctacacgca gttcagccag aacgtgctga aggacgagaa cgagcaggcg gtcacgatcg
    11461 acgacgaggc gggcctggcc ggcctgtcgg ccgcgatccg ggacgggctc gcgcaggagg
    11521 ccgagtcccg cgggcagaaa ggcaagtggg ccgtagcgaa cacgcgctcg agcgtcgagc
    11581 cgttcctgac gtacgccgag aaccgcgcac tgcgcgagaa ggtctggcgc atgttcgtga
    11641 tgcgcggcga caacggcggc ccgaccgaca acaacgcgac gatcacgcgc atcctgcagc
    11701 tgcgcgccga acgcgcgaag ctgctcggct tcccgaccca cgcgcactgg cgcctcgaga
    11761 actcgatggc gaagacgccc gagcgcgcga tggagctcat gcaggccgtg tggccgccgg
    11821 ccgttgcccg cgtgcacgag gaagtcgccg acatgcagaa gatcgcggac gccgagcagg
    11881 ccggcatccg catcgagccg tgggactacc gctactacgc cgaaaaggtc cgcaaggcga
    11941 agtacgacct cgacgagaac gagctcacgc cctacatgca gctcgagaac ctgcgcgaag
    12001 gcatgttcta catggcgaag caactgttcg ggttcacgtt caagcaggtc gaggccgcca
    12061 aggtacccgt gtaccacccg gacgtgcgcg tctgggaggt gaacgatccg cagggcaagc
    12121 tggtcgggct ctggtacttc gatccctacg cgcgcaaggg caagcgctcc ggcgcgtgga
    12181 tgaacgccta ccggacccag gagcggttcg atcgcgaagt caggacgatc gtctccaaca
    12241 actcgaattt cgtgaagggc aagccgggcg agcccgtgct gatcagctgg accgacgccg
    12301 aggtgttgtt ccacgagttc ggccatgcgc tgcacggcct gagctccgac gtgaactacc
    12361 cgtcgctggc cggcacggcg gtgccgcgcg actacgtcga gttcccgtcg cagctgctcg
    12421 agcactggct ctccacgccg gagatcctcg acaagtacgc gctgcacgtc gagaccggca
    12481 agccgatccc gcgcgagctc gtcgccaaga tcgagtcggc tgacaagttc aacaagggct
    12541 tcgacacggt cgagtacctg tcgagcgcgc tcgtcgacat gaagctgcac ctggccgggg
    12601 cgcagaagat cgatccggac aagttcgagc gcgagacgct cgccgcgctc gggatgccga
    12661 gcgagatcgt gatgcgtcat cgcacgccgc agttcaacca cgtgttctcg ggcgacggct
    12721 attcggccgg ctattacagc tacctgtggt cggacacgat cacggcggac gcatgggaag
    12781 cgttcacgga agcgggcggc gcctgggaca agcaggtcgg caaccggctc aagcggctgc
    12841 tcgcgctcgg caacaccgtc gatccggcgg acgcgtaccg cgccttccgt ggccgcgacc
    12901 cgggcatcga cgcactgatg cgcaagcgcg ggttcccggt gaaggactcg tcgcgggctt
    12961 cgacgtcggc ggccccggag gccaagaaga cgcactgacc gttgcagcga cgaccgggtt
    13021 tccttcggca cgcccgtgcc gggggaaacc cgtcggctcg gtcacgcgcc gggcgcgagc
    13081 gcctcgctga tcacgaacgc gtattcctcc gcgatctcgc gcaggtgctc gaaccggccg
    13141 gacttcccgc cgtggccggc ctccatgttg gtgcgcatga cgagcagccg ccgatcggtc
    13201 ttgcagcgcc gcagcttcgc cacccacttc gcgggctcga agtactgcac ctggctgtcc
    13261 cagagcccgg tggtgacgaa catcgccgga tagtcctgct gggccacgtt gtcgtacggc
    13321 gagtacgaca gcatgtagtc gtagtacgcc tgctcggccg gattgcccca ctcgtcgtac
    13381 tcgttggtgg tgagcggaat gctctcgtcg agcatcgtgg tcacgacgtc gacgaacggc
    13441 acgtgcgcca cgatcccgcg gtagagctcg ggcgccatgt tcgcgacggc cgccaccagc
    13501 aggcctccgg cgctgccacc gcgggagaag acacggcgcg gatcggcgta gcgttccgcg
    13561 acgaggtagc gcgtgacgtc gatgaagtcc tcgaagctgt gcttcttctg caactggcgt
    13621 ccggcctcgt accagcgtcg cccacgttcc tggcctccgc gcacctgcgc gatcgcatag
    13681 acgaacccgc ggtcgagcag cgaaagggtc gtggacgaaa agaccgggtc tgacgagatg
    13741 ccatacgcgc cgtagccgta ctggagcagc ggcgcagtgc cgtcgcagcg gaacgtgtcc
    13801 ttgcggtaga cgagcgcgac gggcacgcgc tcaccgtcgc gcgcgggcac ccacaggtgc
    13861 tcggtgcgat atcgggaagg gtcgaagtcc ccgagcaccg gctcgcgctt gagcagcgtc
    13921 ctctggccgc tgcgtacgtc gtagtcgtag gtcgtgtacg gcgtcgtgag cgacgtgtag
    13981 gtgtagcgca cgacgtggct gtcgatctcc tcgttcgcgc cgagcgccat gcggtacgcc
    14041 ggctcgtcgg actcgacgtg gaactcccgg ccgccgagcc agggacggat gcgcaccttg
    14101 cgcagcccgt cgacgcgctc ctcgagcgcc aggaaatcgc ggaagacgtc gaatccggcg
    14161 atgtagacgt cgtcgcggtg cggcacgacc tcccgccagt ggctgcggtc ggccggcgcg
    14221 tcgaccggcg cctccatgat ccggaaattg cgggcatccc agttggtgcg gatgatccag
    14281 cgcccgtcga ggtggtccgc cgagtactcg tggcctcgtt cgcgcggcaa cagcacacgg
    14341 aaacgcagtt cggggtcgtc cgtccgggca tagcgcagct cggacgtgat cgtgctgctc
    14401 gagtggacca gcacgtagcg gcggtccttc gacagctgga ccgaagtgta gaacgactcg
    14461 tcctgctctt cgtagacgag cgcgtcctgc gccggatcgg tgccgagcac gtgcacgcgg
    14521 acgcgcaggc cgagcagcgt gaccggatcc ttctcgacgt agaacacgcg ccggttgtcg
    14581 gccgcccagg ccatgccttc ctcgacgttc ggcacggcgt cgggcatgag ctggccggtc
    14641 tccagggact tgaagcgcag cgtgtgctgg cggcgcccga ccgtgtcctc ggtgtacgcg
    14701 agcagccggt tgtcgtcgct gacctcgagc gcgccgatgt cgtagtagtc gagccccttc
    14761 gcctgttcgc gcaggtcgac cagcacctgc tcgggcgcct cgaggctgcc gggacgccgc
    14821 gcgaagatcg ggtattcgtc gccggtctcg aaccgacggt agtagtacca gccgtggtcg
    14881 cgatacggca cgctcgaatc gtcctgcagg atgcgcccga cgatctcgcg gtagagctcc
    14941 tgctcgagcg gacgcatcgg ggccgtcacc gcatcgcggt aatcgttctc ggccttgagg
    15001 tacgcgagga cctcgggtga ggtgcgcgtg tcgtcgcgca gccagtaata atcatcgaca
    15061 cgcgtgccgt gcggcgagct cacgacgtgt ggacgcatcg ccgcgacggg aggcgtcggg
    15121 cccgtcttcg cgagcttggc ggcgccgtcg gccatgacga ggacttcgtt cagacggccg
    15181 ccggtcccat gcgcacgggg tgcgtgggtc gtgagccgct ggactggtac gccggcagca
    15241 tcttcggcac gagcttgccg agccggtcct gcctcgatgc atcgcttggg tgcgtgctca
    15301 ggaactccgg cggcgcgccg cccccggcct tgcccatctt ctgccacagc gtgatggccg
    15361 cccgcgggtc gtagcccgct ttggccgcca gctcgatgcc gatccggtcg gcctcgttct
    15421 cggacgtgcg gctgttgggc aactgcacgg ccagctgggc cgccgcggcc gcgagcgcca
    15481 tcgactggcc cgggttgtcg gacagcacgc ccgcggcgag gacgcccgcg ttgctcgcga
    15541 gcgccatcga catgcgctcg gcggtgtggt tcgcgagcgc gtgcgcgatc tcgtggccca
    15601 tcacctgcgc gagttcgtcg tcggtggggt cgatcttctg gatcagaccc gtgtagatcg
    15661 ccatgcgacc tccggccatg caccacgcat tgatggtctt cggatcgtcg atgacctgca
    15721 cgctccactg ccactgcgcg ctctcgggac gcatccgcac ggcctggccg accagtcgct
    15781 cggtgatctg ctcgatgcga ttcaacagtc gcggatcggt gacgagcttg ccctcctgct
    15841 tgtacttgcc gatctcctgc gcgtaggcct gccgtgaagc gctgatcgcc tgctgctcgg
    15901 acacgagcat cagctgcttg cggccggtgg ggctcgttgc gcaggcagcg acgatcagcg
    15961 acgtcgagaa ggcgagcaaa ggcttgtgaa agtccatccg agcatcctgt cttggccgcg
    16021 ggacggcgaa atcatgcacc gcggcgccgg gcgcggatag gcgacgggcc ggtcaatacc
    16081 gtgcttcgtc gtgtcagccg caacctcctc ggcgccaacg gcctgccaat cggcgcggat
    16141 ggcgccccat gtcagcgggc gcggactaat tgtgagccgc aacgcttatt gcccggtccg
    16201 gccatgcgcg ccccgatccg gccacacctg ggtcccggga acgccgtgaa cccgtccatg
    16261 ggggctccgg ccgcgacatc cctgtcgcgg acggtcccgg gacccaggtc cggccggatc
    16321 gggacgcgcc gtc
//
LOCUS       DASPFR010000247        10359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_316454, whole genome shotgun sequence.
ACCESSION   DASPFR010000247 DASPFR010000000
VERSION     DASPFR010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10359
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316454"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VD737_06520"
     CDS             <1..511
                     /locus_tag="VD737_06520"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HXW10249.1"
                     /translation="GEGCVMIGFGNLRLCVVLACVALASGCASLATRDPLNVTVAGIE
                     GLPGEGMELRLLVKLRVQNPNDAPVEYDGIAVDLDVQGRSFASGVSAETGVVPGFGEA
                     VVSVPVTVSMLRMARQVMGMLDGERVDKVTYVMRGKLSGTTAFTAHRFSVSGDFSMPK
                     DASQGTDPT"
     gene            522..1133
                     /locus_tag="VD737_06525"
     CDS             522..1133
                     /locus_tag="VD737_06525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HXW10250.1"
                     /translation="MTLLSLFPTRVYSSRLQSTGAVAFNARLLRECEQYRADDAAGRA
                     WSRERYPGGYTSYGSLNRMHRLSPTFAQLDRRLRRHVSAYARALEYDLAARKLEMTDC
                     WFNIMPRGVTHGLHLHPLSTISGTYYVRTPRGVPGLKLEDPRLDRYMAAPPRRADARQ
                     QPWVTVPVEAGMLLLFESWLRHEVPPNPVAAERVSVSFNYNWF"
     gene            1159..2337
                     /locus_tag="VD737_06530"
     CDS             1159..2337
                     /locus_tag="VD737_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HXW10251.1"
                     /translation="MNDQRVPIPRDADDDYTDAMARLRREFVAERTGAKLDHVGQHSV
                     SPSQVAGNIENFFGVAQVPIGLAGPLRIDGEHAQGDFYVPLATTEGTLVASYSRGMRV
                     LSESGGVKVTVVKHSMQRAPVFHFRDARQARDFGHWLVEHFQSIKAAAESTTRVGKLL
                     DIEQYALGNLLYTRFNYTTGDAAGQNMTGKATWAACEWIHAQHPLRPRYILSGSMDTD
                     KKHSAVNTLRTRGKRVIAEATIRNEVIQRLMRVDTATLFRARQISMAGSFMAGSAYNG
                     PHAANGIAAMFIATGQDVANVAESHAGITYAQLLDDGDYYWSVTLPSLIVASYGGGTG
                     LPTQRECLEMLGCYGAGKADRLAEIVGAVVLAGDVSLGSAVLAGDWVSSHDRLGRNRP
                     "
     gene            2364..2894
                     /locus_tag="VD737_06535"
     CDS             2364..2894
                     /locus_tag="VD737_06535"
                     /inference="COORDINATES: protein motif:HMM:NF014136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXW10252.1"
                     /translation="MSNEDAPILNLDEASGHEGSHGEQFAYSWTSLAAPLGAKAIGAN
                     VTRIPPGKAAFPLHHHHANEEHFFVLSGQGTLRVGERTWPVRPHDYVVCPAGGPGTAH
                     QLVNTGREDLVFLAVSTVVVPDVVGYPDSAKTGVRLGPEPGARFLVEDRSKGQVGYWD
                     GEDGRQVRALVERNQS"
     gene            complement(2885..3184)
                     /locus_tag="VD737_06540"
     CDS             complement(2885..3184)
                     /locus_tag="VD737_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10253.1"
                     /translation="MDRSNARPRLRYRGVEVVAPGDACPAARALHGLRLLTAEAPSRL
                     PLGDCDRPGTCRCVYRHFDDRRQGPRRENEHAQIPLAHLGPERRRWRGRRDSDYD"
     gene            3333..3731
                     /locus_tag="VD737_06545"
     CDS             3333..3731
                     /locus_tag="VD737_06545"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HXW10254.1"
                     /translation="MTIHVVRLGSERRPGEGLRIGTVRRPPRGVPKADFARRNFYDVW
                     LPTLAPSEPLLKQALGAVANDDARAWRQFVRRYRAEMKAPDARALLDTLAGLSHHGDF
                     SVGCYCADESRCHRAILRELLAERGARLAP"
     gene            3786..4370
                     /locus_tag="VD737_06550"
     CDS             3786..4370
                     /locus_tag="VD737_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HXW10255.1"
                     /translation="MEWSRIAAACLVAAVPTLALADAPTRATAEFQGCGETKVTGTAT
                     LKEQVTAEGVKEVTVEMKVQGLADGKHAVHIHEVGKCEPCAAAGGHHDPGPFGQSRPD
                     TAADTTPATDINHPFHMGDLINLEVKNGTGTLKHVTNRVTLSPGRLSVFDQDGSSIIV
                     HTQPDTYCDEETDLKKGCAGGAREACAVLKPASR"
     gene            4652..5593
                     /locus_tag="VD737_06555"
     CDS             4652..5593
                     /locus_tag="VD737_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HXW10256.1"
                     /translation="MSIVTSRPLFVVLNTSSGRHDGEDDAATTIAGVLGRAGCAHELR
                     PVGDPSALEATARRAAEDARASGGVLVGAGGDGTLNTIAHAALEQGVEYGVIPRGTFN
                     YFARAHGIPTDTEEATRALLEAEVEPVPVGLVNGRPFLVNASVGLYPEALEQREEQKQ
                     RHGRSRPVALWAALLTILQGHHPIQVRLERGGREVQLSTLTLFVGLNPLQLEQMGWDG
                     TPVEQGRLAAIVLKPVTTPRLLWLLARGALGRLATAQGVESFTFVRLTVTPVSRARRV
                     KVATDGETTLMQAPLEFELASQPLKLLKPVTVAEPAA"
     gene            5590..6396
                     /locus_tag="VD737_06560"
     CDS             5590..6396
                     /locus_tag="VD737_06560"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXW10257.1"
                     /translation="MTTVLQVSDTHFGTEQSPVVDALLDLAARERPDLLVISGDVTQR
                     ARRRQFQAAQAFVERVAATRALAIPGNHDIPLFNVFARLFAPYAGYHRAFGADLEPEF
                     DGPDLLVACVNTTRPRRHKDGEVSRAQVERVAGRLRRAHRSQLRVVVVHQPVMAARSD
                     DERNLLHGRDDAVRAWVAAGADVVMGGHIHWPYACSLQIGFRGLPRDAWIVQAGTAVS
                     SRTRDGVPNSVNVLRCEPARAGRRCRIERWDYSVEERAFGRVESHDVEFA"
     gene            complement(6643..7182)
                     /locus_tag="VD737_06565"
     CDS             complement(6643..7182)
                     /locus_tag="VD737_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10258.1"
                     /translation="MNGTSLARLLQESRRLSDAAHDARVEWLADAGATALELRLLDRL
                     ESAGGPASPRRLARPLLCTVSDVEGPLRAMERRAWIAEVPVARGGPPAFEIRPLGRRA
                     LASFRLAERELGAALERVLDEQELRAALSVLAAVRGRLQGRQRRRRTRGRGATGRADF
                     DRPRGASATWVVVAGASGA"
     gene            complement(7179..7868)
                     /locus_tag="VD737_06570"
     CDS             complement(7179..7868)
                     /locus_tag="VD737_06570"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HXW10259.1"
                     /translation="MTRHLTPARWIAAAFLLQGVIGPVVNFGLLAPALSAPPGFLQNA
                     ASHAWDVQVATLLALVGAACSVGVGLVLLSVARAETARGLALIVLVLTVLAFAASVLE
                     GTAIRGMLALSRAFHESGAADAGAYEPLRATLQAFRRNAHYIVLMLSCGAFIALYLCL
                     LRLAWIPRLLAWAGVLSAVLSIGAALAPLLGGRIVFALLAPLGLCQLALIAWLFAARS
                     ANAVRTQEVHA"
     gene            complement(7865..8611)
                     /locus_tag="VD737_06575"
     CDS             complement(7865..8611)
                     /locus_tag="VD737_06575"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HXW10260.1"
                     /translation="MDEDALHRSPQSAARIAGALYLVIVFVGVFGQALVRDRLVVSGD
                     AAATAQNILASATMWRASVSAELAYLALAVVVDVLLYRLLRPVNPTIALLAFALNLVS
                     IATEVVGRFMLLAPLVLLSDAPYLQPIAAEARQALAYAFLRLHDHGFALALIFFGAVC
                     LAWGYLIRTSGFLPPVLGWLMQLAGACYLVNSFALLLAPPLAGALFPAILLPAFVAEL
                     GLALWLVARGVDAGAWRSVARSSRTAEALA"
     gene            8800..9882
                     /locus_tag="VD737_06580"
     CDS             8800..9882
                     /locus_tag="VD737_06580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXW10261.1"
                     /translation="MNQTVSSLATGRGPASAMSWPRVKFTLIWSAVLGALLSLPMETS
                     TWIVVIRAIVVGVAAMLAFSLFERWPGREPRWWPRWVLQLVAIVVVVPVAALFAYWLT
                     TGGHPNFSGEQKRVVGYMMLTFMGVFFAPWIALGALLRQREAFARTQALAFDLERSEL
                     ERKASDARLRLLQAQVAPHFLFNTLANVQALVDAGSPQASAVLKSLIAYLRAAVPRLD
                     DPATTLDHEADLVRAYLEVMRMRMPDRLTYALQVDPSARRLACPPMTLLTLVENAVRH
                     GIDPGEEGGHIDVHVEVRAGRCHLRVADTGVGLRAAGSGLGTGLSTLKERLALAFRGD
                     AHLKLTEREPHGVLAEVDFPAREVSA"
     gene            9879..>10359
                     /locus_tag="VD737_06585"
     CDS             9879..>10359
                     /locus_tag="VD737_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXW10262.1"
                     /translation="MSAAHPTALIADDEPLLRDSLARMLAGAWPGLEVVAQARNGREA
                     VEQFERLRPDVCFLDVHMPGLSGVEAAARIGRRAHLVFVTAFDQYAVQAFAHGVLDYL
                     VKPVEPARLADTVARLQERLRAAEPATNTDALLRELALQLRQGPAQAPPLRWIRASVG
                     "
BASE COUNT         1598 a         3618 c         3703 g         1440 t
ORIGIN      
        1 cggggagggc tgcgtcatga tcggcttcgg caatttgcgg ctgtgcgtcg tgctcgcctg
       61 cgtggcgctg gccagcggtt gcgcgtcgct ggcgacccgc gatcccctca acgtcacggt
      121 ggccggcatc gaaggcctgc cgggcgaagg catggaactg cgcctgctcg tgaagctgcg
      181 cgtccagaac cccaatgacg ccccggtcga gtacgacggc atcgcggtcg acctcgacgt
      241 ccagggtcgc tcgttcgcga gtggcgtcag cgccgagacg ggcgtggtgc ccgggttcgg
      301 cgaggcggtc gtgagcgtgc cggtcacggt gtcgatgctg cgcatggcca ggcaggtgat
      361 gggcatgctc gacggcgagc gcgtcgacaa ggtgacctac gtcatgcgcg gcaagctgag
      421 cggcacgacc gcgttcaccg cgcatcgctt ttcggtgagc ggggatttct cgatgccgaa
      481 ggacgcttca cagggaaccg acccgacatg acgctgcagc cgtgacgctg ctgtcgctgt
      541 tcccgacgcg ggtctattcg agccggctgc agtcgaccgg cgcggtggcg ttcaacgcgc
      601 gcctgctgcg cgagtgcgag cagtaccggg ccgacgacgc ggcggggcgc gcatggtcgc
      661 gtgagcgcta cccgggcggc tacacgtcgt acggctccct caaccgcatg cacaggctgt
      721 cgcccacctt tgcgcagctc gatcgcaggc tgcgcaggca cgtgtcggcg tacgcgcgcg
      781 cactcgagta cgacctcgcc gcacgcaagc tcgaaatgac cgactgctgg ttcaacatca
      841 tgccgcgcgg cgtcacgcac ggcctgcacc tgcacccgct gtcgacgatc agtggcacct
      901 actacgtgcg cacgcccagg ggcgtgcctg gactgaagct cgaggatccg cggctcgacc
      961 ggtacatggc cgcgccgccg cgccgcgccg atgccaggca acagccgtgg gtcacggtcc
     1021 cggtcgaggc cggcatgctg ctgctgttcg agagctggct gcgtcacgag gtgccgccga
     1081 acccggtcgc cgcggagcgg gtcagcgtga gcttcaacta caactggttc tgagagagcc
     1141 gcggacgagg agccttccgt gaacgaccag cgcgtgccga tcccccgcga tgccgacgac
     1201 gactacacgg acgcgatggc gcgactgcgg cgcgaattcg tcgccgagcg caccggggcg
     1261 aagctcgacc acgtcggcca gcactcggtg tccccgtcgc aggtcgcggg caacatcgag
     1321 aacttcttcg gcgtcgcaca ggtgccgatc gggctcgccg gtccgctgcg gatcgacggc
     1381 gagcacgcgc agggcgactt ctacgtgccg ctcgcgacga ccgagggcac gctggtcgcg
     1441 agctacagcc gcggcatgcg cgtgctgtcg gagagcggcg gcgtcaaggt cactgtcgtc
     1501 aagcactcga tgcagcgcgc gccggtgttt cacttccgcg acgcgcgcca ggcccgcgat
     1561 ttcgggcact ggctcgtcga gcacttccag tcgatcaagg ccgcggccga gtccacgacc
     1621 cgcgtgggca agctgctcga catcgagcag tacgcgctcg gcaacctgct ctacacgcgc
     1681 ttcaactaca ccacgggcga tgcggccggc cagaacatga cgggcaaggc gacctgggcc
     1741 gcctgcgagt ggatccacgc gcagcatccg ctgcgaccgc gttacattct ctcgggctcg
     1801 atggacaccg acaagaagca ctccgcagtc aacacgctgc gcacgcgcgg caagcgcgtg
     1861 atcgccgagg cgacgatccg caacgaggtg atccagcgcc tgatgcgggt cgacacggcc
     1921 acgctgttcc gcgcgcgaca gatcagcatg gcgggctcgt tcatggccgg atcggcgtac
     1981 aacgggccgc acgcagcgaa cggcattgcc gcgatgttca tcgcgaccgg ccaggacgtc
     2041 gccaacgtcg ctgaatcgca cgcgggcatc acctacgcgc agctgctcga cgacggtgac
     2101 tattactggt cggtgacgct gccctcgctg atcgtggcga gctacggcgg tggcacgggg
     2161 ctgcccacgc agcgcgagtg cctcgagatg ctcggctgct acggcgcggg caaggccgac
     2221 cgcctggccg agatcgtggg cgcagtcgtg ctggccggcg acgtctcgtt gggctctgcc
     2281 gtactggcgg gcgactgggt gagcagccac gaccggctcg gacgcaaccg tccctgaagc
     2341 gggccctccg cgggagcgag tacatgtcga acgaagatgc gccgatcctg aacctggacg
     2401 aggcgtcggg acatgaaggc agccacggcg agcagttcgc ctactcgtgg acgtcgctgg
     2461 cggcgccgct cggcgccaag gcgatcggtg ccaacgtcac gcggataccg cccggcaagg
     2521 ccgcgtttcc gctgcatcac caccacgcca acgaggagca cttcttcgtg ctgagcgggc
     2581 agggcacgtt gcgcgtgggc gaacgcacgt ggcccgtgcg cccgcacgat tacgtcgtct
     2641 gcccggcggg tggccccggg acggcgcacc agctggtcaa caccggtcgc gaagacctgg
     2701 tcttcctcgc ggtctcgacg gtcgtggtgc cggacgtggt cggttatccc gactcggcca
     2761 agaccggcgt gcgcctcggt ccggagccgg gggcgcgatt cctggtcgag gatcgcagca
     2821 agggccaggt gggttactgg gacggcgaag acgggcggca agtgcgcgcg ctcgtcgagc
     2881 gaaatcagtc gtagtcgctg tcgcgccgac cgcgccagcg acgccgctcc ggaccgaggt
     2941 gcgcgagcgg gatctgggcg tgctcgttct cgcgacgcgg tccctggcgc cggtcgtcga
     3001 agtgccggta gacgcagcgg cacgtgccgg gacggtcgca gtcccccagc ggcaaccgcg
     3061 agggcgcctc tgccgtcagc agtcgcagcc cgtgcagggc tcgtgcggcc gggcaggcgt
     3121 cacccggggc gacgacttcc acaccgcgat acctgagccg gggcctggcg ttgctgcgat
     3181 ccatgctgtc acctgcgcca ttgtgtcgtc gcgatccggc gcgtctggcg gagcggagcc
     3241 cgtaaccgtg cagggcaggt ccgtcggcca aagacgcagg gacgggggaa tcacctgcgg
     3301 gtaagctctg cagcccgctt cggagaacgc tgatgacgat tcacgtcgtg cgactcggca
     3361 gcgaacgccg gcccggcgag gggctgcgta tcggtacggt gcgccggccg ccacgcggcg
     3421 tgcccaaggc ggatttcgcg cgccgcaact tctacgacgt ctggctgccc acgctcgcgc
     3481 cgagcgaacc cctgctcaag caggcgctcg gcgcggtcgc gaacgacgac gcacgcgcct
     3541 ggcggcagtt cgtgcggcgc tatcgcgccg aaatgaaggc ccccgacgcg cgcgcgctgc
     3601 tcgatacgct cgccggcctc tcgcaccacg gcgacttctc ggtggggtgt tactgcgcgg
     3661 atgaatcgcg ctgccaccgg gcgatcctgc gcgagctgct ggccgagcgg ggcgcccgtc
     3721 tcgcgccgtg aattgcgcgc tatagttcac gcccggttcc ggcctgacga tcaaaggagt
     3781 tgcagatgga atggtcccga attgccgccg cgtgcctcgt cgccgccgtc cccacgctcg
     3841 ccctcgccga cgccccgacc agggccaccg ccgagttcca gggttgcggc gaaaccaagg
     3901 tgaccggcac cgcgacgctc aaggagcagg tgacggccga aggcgtcaag gaagtcacgg
     3961 tcgagatgaa ggtgcagggg ctcgccgacg gcaagcacgc ggtgcacatc cacgaggtcg
     4021 gcaagtgcga gccctgcgcg gccgccggcg gccaccacga tcccggtcca ttcggtcagt
     4081 cgcggccgga caccgcggcg gacacgacgc ccgcgacgga catcaaccat ccgttccaca
     4141 tgggcgacct gatcaacctg gaggtcaaga acggcacggg gacgctcaag cacgtgacca
     4201 atcgcgtgac gctgtcgccg ggccgcctga gcgtcttcga tcaggacggc agctcgatca
     4261 tcgtgcacac gcagcccgac acgtactgcg acgaggagac ggacctcaag aagggttgcg
     4321 cgggcggcgc acgcgaagcc tgcgcggtct tgaagcccgc cagccgctag cgcacacacg
     4381 cgggcggcgc tcgcgacgag cgtcgctccg ccttgcgcca ggcgccagtc ggggcacctg
     4441 acgtgggaga catcgatctc gttcagcggg ccgtagcgcg tgctcgcgta gccgagcgcg
     4501 ggcatcgtcg ccgagtgtca tggcggagag tctcgtgacc cgccgcgcgc acgggatgtc
     4561 ggctggtacg cctcgcctga gtaggagcgg acgcagtgaa gcctgccgct ttgctccttc
     4621 cgccgcgggg cgacatcacc agaatcgcgg cgtgagcatc gtcacttcgc gtccgctgtt
     4681 cgtcgtgctg aacacctcgt ccggccgaca cgacggtgag gacgacgcag cgacgaccat
     4741 cgcgggcgtg ctgggccgcg ccggctgcgc gcacgagttg cggcccgtcg gcgacccctc
     4801 ggcgctcgaa gccactgccc ggcgcgccgc cgaggacgcc cgcgcgagcg gtggcgtgct
     4861 ggtcggcgcc ggcggcgacg gcacgctcaa cacgatcgcg cacgcggcgc tcgagcaggg
     4921 cgtcgaatac ggcgtgatcc cgcgcggcac gttcaactac tttgcccggg cgcacggcat
     4981 cccgaccgac accgaggagg cgacgcgcgc attgctcgag gccgaggtcg aaccggtgcc
     5041 ggtcgggctc gtgaacgggc ggccgttcct cgtcaacgcg agcgtcgggc tctatccgga
     5101 agcgctcgag cagcgcgagg agcagaagca gcgtcacggc cgcagccggc ccgtcgcgct
     5161 gtgggcggcg ctgctgacga tcctgcaggg ccaccacccg atccaggtgc gcctcgagcg
     5221 cggcggacgc gaggtgcagc tttcgacgct gacgttgttc gttggcttga atccgttgca
     5281 gctcgagcag atgggctggg acggcacacc ggtggagcag ggccgcctgg cggcgatcgt
     5341 gctgaagccc gtgaccacgc cgcgcctgct gtggctgctc gcacgcggcg cgctgggacg
     5401 cctggccacg gcgcagggcg tcgagagctt tacgttcgtt cgcctcacgg tgacgccggt
     5461 cagccgggcg cgccgggtca aggtggcgac ggacggcgag accaccttga tgcaagcgcc
     5521 gctcgagttc gaactggcat cgcaaccgct caagctgctg aagccggtca ccgtcgcgga
     5581 gccggcggcg tgaccacggt gctgcaggtc tcggacacgc acttcggcac cgagcagtcg
     5641 ccggtcgtcg acgcgctgct cgacctggcg gcccgggagc gtccggacct gctcgtgatc
     5701 tccggcgacg tcacgcagcg cgcccggaga cggcagttcc aggcggcgca ggcgttcgtc
     5761 gagcgcgtcg cggcgacgcg ggccctggca atcccgggca accacgacat tccgctgttc
     5821 aacgtcttcg cccggctgtt cgcgccatac gccgggtatc accgagcgtt cggggcggac
     5881 ctcgagccgg agttcgacgg accggacctg ctcgtcgcct gcgtcaacac gacacgaccg
     5941 cgtcgacaca aggacggcga ggtctcgcgg gcccaggtcg agcgcgtcgc ggggcgcttg
     6001 cgacgggccc accgctcgca gctgcgcgtc gtggtcgtgc accagccggt catggccgcc
     6061 cgttcggacg acgagcgcaa cctgctgcac ggacgcgacg acgccgtgcg agcctgggtc
     6121 gccgccggcg ccgacgtcgt gatgggcggg cacatccact ggccttatgc gtgctcgctg
     6181 cagatcgggt tccgcggcct gccgcgcgac gcctggatcg tgcaggcggg gacggccgtg
     6241 tcgtcccgca cgcgcgacgg cgtgccgaat tccgtcaacg tcctgcgttg cgagcccgcg
     6301 cgcgccgggc gtcgttgccg gatcgagcgc tgggattact ctgtcgagga acgcgccttc
     6361 gggcgtgtcg aaagccacga cgtcgaattc gcctgagcgt gcgggagcag cgaccatggc
     6421 cgtgatgctg gacaagcccc tcaagcgtga gctcgcggtc ggcaccgacc cgtacatgct
     6481 caccattgct ccggacggac tgaagctcgt gcccaagggc aagcgcatcg gcgtcgaatt
     6541 gcgctgggaa gacctggtca gcgggcaggc tgcactcgcg gtcgcgctga acgcgtcgat
     6601 cgggcgcctg gccgagcggg atcggcgtcc gggtccggcg gctcaagcgc cgctcgcgcc
     6661 cgccacgacc acccaggtgg ccgaggcacc tcgaggccga tcgaaatcgg cacgcccggt
     6721 cgcgccgcgg ccgcgcgtac ggcgtcgccg ctgacggcct tgcagcctgc cgcgcaccgc
     6781 ggccagcacc gagagcgccg cccgcagttc ctgctcgtcg agcacgcgtt cgagcgccgc
     6841 cccgagttcg cgttcggcca ggcggaagct cgcgagcgcg cggcgaccga gcgggcggat
     6901 ctcgaacgcc ggcggcccgc cgcgggccac cgggacttcc gctatccacg cgcgccgctc
     6961 catcgcgcgc aaggggccct cgacgtcgga caccgtacac agcagcggtc gcgcgagccg
     7021 gcgcggcgag gccgggccac cggccgactc gagccggtcc agcaggcgca gttcgagcgc
     7081 cgtcgccccg gcgtcggcga gccactcgac gcgcgcgtcg tgcgcggcgt cactcagtcg
     7141 ccgagattcc tgcagcagcc tggccagcga agttccgttc acgcgtgtac ctcctgtgtc
     7201 cgaacggcgt tcgccgaccg tgcagcgaac agccaggcga tgagcgccag ctggcacagc
     7261 ccgagcggtg cgagcagcgc aaacacgatg cgcccgccga gcaggggcgc gagcgcggcg
     7321 ccgatggaca gcaccgcgga gagcacaccg gcccatgcca gcaggcgcgg aatccatgcg
     7381 aggcgcagca ggcacaggta aagcgcgatg aaggctccgc aggacagcat caggacgatg
     7441 tagtgggcat tgcgtcgaaa cgcctgcagc gtcgcgcgca ggggttcgta cgcgccggca
     7501 tccgccgccc ccgactcgtg gaacgcccgg ctcagcgcga gcatcccgcg gatcgccgtg
     7561 ccctcgagca ccgacgccgc gaatgccagc accgtcagca cgaggacgat caacgcaagg
     7621 ccacgggccg tctcggcccg cgccaccgac agcaggacca gcccgacgcc gaccgagcac
     7681 gccgccccga cgagcgcgag cagcgtggcc acctgtacgt cccacgcgtg cgatgctgcg
     7741 ttctgcagga agccgggggg cgccgacagg gcaggagcca gcagtccgaa gttgacgacc
     7801 ggcccgatga ctccctgcaa caggaacgcc gcggcgatcc agcgtgccgg cgtgagatgt
     7861 cgcgtcatgc cagagcctcg gcggttcgtg agcttcgtgc caccgagcgc cacgcccccg
     7921 cgtcgacgcc gcgggccacg agccatagcg cgagcccgag ttccgcgacg aacgcaggca
     7981 gcaggatcgc cggaaagagc gcaccggcga gcggcggcgc aagcagcaag gcaaagctgt
     8041 tgacgaggta acacgcgccg gcgagttgca tgagccagcc gagcaccggc ggcaggaagc
     8101 ccgaggtgcg gatcaggtag ccccacgcga ggcagacggc gccgaagaag atcagcgcga
     8161 gcgcgaatcc gtgatcgtgc agtctcagga aggcgtacgc gagcgcctgg cgcgcctcgg
     8221 ccgcgatcgg ctgcaggtag ggcgcatcgc tgagcagcac gagcggcgcg agcagcatga
     8281 aacggccgac gacctcggtc gcgatcgaaa ccaggttcag ggcgaacgcg agcagtgcga
     8341 tcgtgggatt cacgggccgc agcagccgat agagcagcac gtcgaccacc acggcgagtg
     8401 cgagatacgc gagctccgcc gacacgctgg cccgccacat cgtggccgag gccaggatgt
     8461 tctgcgccgt cgcggccgca tcgcccgaca ccacgaggcg atcccgtacg agcgcctggc
     8521 cgaacacacc gacgaacacg atgacgaggt acagcgcgcc ggcgatgcgt gcggcggatt
     8581 gcggcgaccg atgcagtgcg tcctcgtcca tgcgtccccc gtcgacctgg ttcgtgaggg
     8641 gggatgatcg ggcccggcca cgcgacgtgc caaccgcatg cgacgagtgg cggtatgcgc
     8701 gtcccgggat acgaccggcg cgcgggaacg gccgaaaacc ccgtgtggcg aggcgcccgc
     8761 gtgcggggcg acccgatacg gtttcgatat agtcgggcca tgaaccagac cgtctcgtct
     8821 ctcgcgactg gacgcggccc tgcctcggcg atgtcctggc cgcgcgtcaa gttcacgctg
     8881 atctggtcgg ccgtgctcgg agcgctgctg tcgctgccga tggaaacgtc cacctggatc
     8941 gtcgtgattc gcgcgatcgt ggtgggcgtc gccgccatgc tcgccttcag cctgttcgag
     9001 cgctggccgg gccgtgagcc tcgctggtgg ccccgctggg tgctgcaact ggtggcgatc
     9061 gtagtcgtgg tgccggtcgc ggcgctgttc gcctactggc tgaccaccgg cggccacccg
     9121 aacttcagtg gcgagcagaa gcgtgtcgtg ggctacatga tgctcacgtt catgggcgtg
     9181 ttcttcgcac cgtggatcgc gctcggcgcg ctgctgcggc aacgcgaggc gttcgcacgc
     9241 acgcaggcgc tcgcgttcga cctcgagcgc agtgagctcg agcgcaaggc ttccgacgcc
     9301 cgcctgcgac tgctgcaggc gcaggtcgcg ccccacttcc tgttcaacac gctcgcgaac
     9361 gtgcaggcgc tcgtcgacgc cggttcgccg caggcctcgg ccgtgctgaa gagcctgatc
     9421 gcctacctgc gcgccgcggt gccgcggctc gacgatccgg ccacgacgct cgaccacgag
     9481 gcggacctcg tgcgggcgta cctcgaggtc atgcgcatgc gcatgcccga ccgactcacc
     9541 tacgcgctgc aggtcgaccc gtcggcgcgc cggctcgcct gtccgccgat gacgctgctg
     9601 acgctggtcg agaacgccgt gcgccacggt atcgatccgg gcgaggaggg tgggcacatc
     9661 gacgttcatg tcgaagtgcg ggccgggcgt tgccacctgc gcgtggcgga caccggtgtc
     9721 ggcctgcgag ccgcgggctc gggcctcggc acgggcctct cgaccctcaa ggaacggctt
     9781 gcgctcgcct tccgaggcga cgcgcacctc aagctcaccg agcgcgagcc gcacggggtg
     9841 ctggccgaag tcgacttccc ggcgcgcgag gtctcggcat gagcgccgcg cacccgacgg
     9901 cgctgatcgc cgacgacgaa ccgctgttgc gggattcgct cgcacggatg ctggcagggg
     9961 catggccggg gttggaggtc gtcgcgcagg cccgcaacgg ccgcgaggcc gtcgagcagt
    10021 tcgagcggct gcgacccgac gtctgctttc tcgacgtgca catgcccggc ctgagcggcg
    10081 tggaggcggc ggcgcgcatc gggcggcgcg cgcatctcgt gttcgtgacc gcattcgacc
    10141 agtacgcggt gcaggcgttt gcgcacggcg tgctcgatta cctcgtcaag cccgtcgaac
    10201 ctgcgcggct cgcggacacc gtcgcgcgtc tgcaggagcg gttgcgcgcc gccgagcccg
    10261 cgaccaacac cgacgcactg ctgcgcgagc tcgcgctgca gctcaggcag gggccggccc
    10321 aggcgccccc gctgcgctgg attcgcgcct cggtgggca
//
LOCUS       DASPFR010000248         3793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1659711, whole genome shotgun sequence.
ACCESSION   DASPFR010000248 DASPFR010000000
VERSION     DASPFR010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659711"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="VD737_06590"
     CDS             complement(<1..1387)
                     /locus_tag="VD737_06590"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HXW10263.1"
                     /translation="MFSHLRWNFVYQRPQHLLARLAREFRVLYVEEPTHHDGERRLEV
                     IETESGVTVCRPHLPRRAAGFHDDHIADLQFLVRQLLEDTGVEDYAVWFYTPMALPLI
                     DPLRPRAVIYDCMDELSAFLHAPRQIVQRESALLKRADLVFTGGPSLYRSKKERHPSV
                     HCFPSSVDAAHFASARDDERPANRTPTLGFFGVIDERIDLGLVAALADAHPEWKIVMV
                     GPVVKIPEESLPRRANVEYLGQRSYQELPGLVAQWDVCLLPFALNESTRFISPTKTLE
                     YMAAGKPSVSTPINDVVECFGDVVRIAASQETFIAACEASLAETAEQGAERRRRADAA
                     LEKTSWDRTAQAMRGLIDSAWRPVAPPASADHTPVARAGTAPVRAREVETVVIGAGPT
                     GLSAAYHLGRDSVLLEAGSTVGGWCRSIVDNGFTFDHAGHIMFSNDPYVHELYDKLLG
                     DNVHWQNREAWI"
     gene            1694..2770
                     /locus_tag="VD737_06595"
     CDS             1694..2770
                     /locus_tag="VD737_06595"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HXW10264.1"
                     /translation="MHCEVARLEDETLDGERVERLLLRAGSVTLECLGLGCAITRLEA
                     PDRDGQSANVVLGYQDAQAYHADAPREYFGVVVGRVANRIGGARFVLDGHEHRLATND
                     GANHLHGGRRGFDRRVWSVVETREGDAACVRFQRLSPDREEGYPGQLTATVTYTLSPD
                     DSLRIDYEATTTAPTVVNLTQHSYFNLAGEGSGDALEHEIEIDADEVCVVDLGLIPTG
                     EIRPVGGTPFDLREARSIATALSQADPQLRVAGGFDHCFALRGWRGGARTLRRACTLT
                     DPRSGRRLRVDTDQPGVQFYTGNFLDGTVTGRAGRPYARHAGVCLETQHFPDAPNRPE
                     FPSIVLRPGERYATATVLRFDVLP"
     gene            2846..>3793
                     /locus_tag="VD737_06600"
     CDS             2846..>3793
                     /locus_tag="VD737_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10265.1"
                     /translation="MTATTPGATLHPRPQLQRERWISLDGPWRFAFDPERRWDRPADI
                     EDWPLTIRVPFCPESGMSGIGDTGFHPVCWYERDFDLPQLPPNGRLLLHFGAVDYQAR
                     VWVNDYPVMEHEGGHVPFVIDITRAIRPGGTQRVTVQALDDPEDLEKPRGKQDWKLEP
                     HGIWYPRTSGIWQTVWLEAVSETYLRAVKWTPNLERWEVTFHAYIDGMQADETEVRLR
                     LRAGDKLLIDDDYRVVHGEVHRKLGLSDPGIDDFRNEILWSPERPRLLQAEIELLRDG
                     AVIDRATSYTALRSFGVQRDRLMLNGRHYQLRLVLDQGYW"
BASE COUNT          609 a         1275 c         1310 g          599 t
ORIGIN      
        1 agatccacgc ttcgcggttc tgccagtgca cgttgtcgcc gagcagcttg tcgtacagct
       61 cgtgcacgta cgggtcgttc gagaacatga tgtgcccggc gtgatcgaac gtgaacccgt
      121 tgtcgacgat cgaccggcac cagccgccca cggtgctgcc ggcctcgagc agcacgctgt
      181 cgcgcccgag gtggtacgcc gcactgaggc cagtcgggcc cgcgccgatc acgacggtct
      241 cgacttcgcg cgcgcgaacc ggcgcggtgc ccgcgcgcgc gacaggcgtg tggtcggcgc
      301 tggcgggcgg cgcgacgggc cgccacgccg agtcgatcaa cccacgcatg gcctgcgcag
      361 tccggtccca cgacgtcttc tcgagcgccg cgtcggcgcg tcgacgccgc tcggcgcctt
      421 gctccgccgt ttcggccagt gaggcctcgc acgccgcgat gaacgtctct tgcgacgcgg
      481 caatcctgac cacgtcgccg aagcactcga ccacgtcatt gatcggcgtg ctcacgctcg
      541 gcttgccggc ggccatgtac tccagcgtct tggtcgggct gatgaagcgc gtcgactcat
      601 tgagcgcgaa cggcagcagg cacacgtccc actgcgcgac cagcccgggc aattcctggt
      661 agctgcgctg gccgaggtac tcgacgttgg cgcgccgggg cagcgactcc tccgggatct
      721 tcacgaccgg gccgaccatg acgatcttcc actcggggtg cgcgtcggcg agggctgcga
      781 ccagaccgag gtcgatccgc tcgtcgatca cgccgaagaa ccccagcgtg ggcgtgcgat
      841 tggcgggccg ttcgtcgtca cgtgcgctgg cgaaatgcgc ggcgtccact gagctcggga
      901 agcagtgaac cgacgggtgg cgctccttct tggagcgata gaggctcgga ccgccggtga
      961 agacgaggtc cgcgcgcttc agcagcgcgc tttcgcgctg cacgatctgt cgcggcgcgt
     1021 gcaggaaggc cgagagctcg tccatgcagt cataaatgac cgcccgcggc cgcagcggat
     1081 cgatcagcgg caacgccatc ggcgtgtaga accagaccgc gtagtcctcg acccctgtgt
     1141 cttcgagcag ctgccggacg aggaactgca ggtccgcaat gtggtcatca tgaaagccgg
     1201 ccgcgcgtct cggcaggtgc gggcggcaga cggtgacgcc gctctcagtc tcgatgacct
     1261 cgaggcggcg ctcgccgtcg tggtgcgtcg gctcctcgac gtagaggacg cgaaattcgc
     1321 gagcgaggcg cgcgagcagg tgctgtggac gctggtagac gaagttccag cggaggtgag
     1381 aaaacacgag caacgcgcgg ctgtgcatga gcaaattctc caatgttcgg ttcggtcacg
     1441 acgcggctgt cagcgcggct gtgccgggcg gtgaagctat cgcgaacgtg acataacggt
     1501 ccgtcatggt ccggcccgat tcttcgtggg ccgaaactga cgcgctagcg acatcccaca
     1561 cggcagtggg aggtcaccga ctgccgcgct tcgcgtgcgc gagttttcgc cgggtctgtt
     1621 tcacagcaca gtgcgcgacc tgtctcgttc gcctcgccgt cgagggcgcg cgacctcatc
     1681 gtccgaggtg cagatgcatt gcgaagtggc gcggctcgaa gacgagacgc tcgacggcga
     1741 aagggtcgag cgactcctgc tgcgggccgg gtcggtgacg ctcgaatgcc tgggcctggg
     1801 ctgcgcgatc acgcgactcg aggcgccgga ccgcgacggc cagtccgcga acgtcgtgct
     1861 gggctaccag gacgcacagg cttaccacgc cgacgcgcct cgcgagtact tcggcgtcgt
     1921 ggtgggacgc gtcgccaatc gcatcggcgg cgcgcggttc gtactcgacg gccacgagca
     1981 ccggctggcg accaacgacg gagccaatca cctgcacgga ggacgtcgag gtttcgaccg
     2041 gcgcgtctgg tccgtcgtcg agacccgcga aggcgatgcc gcctgcgtgc gctttcagag
     2101 gctgagcccg gatcgggagg agggctaccc tggccagctc acggccacgg tcacgtacac
     2161 gctgtcgccc gacgactcgc tgcggatcga ctacgaggcg accaccaccg cgccgacggt
     2221 cgtgaacctg acgcagcaca gctacttcaa cctggcgggt gaagggtcgg gcgacgcact
     2281 ggagcacgag atcgagatcg acgccgacga ggtgtgcgtg gtggacctcg gactgatccc
     2341 gaccggcgag atccgtcccg tcggcgggac gccgttcgac ctgcgcgagg ctcggtcgat
     2401 cgcgaccgcg ctgtcgcagg cggacccgca gttgcgcgtc gctggcggtt tcgatcattg
     2461 cttcgcgctg cgcggctggc gcggcggtgc acggacgctg cgtcgagcct gcacgctgac
     2521 cgacccgcgc agcggccgtc gcctgcgcgt cgacaccgac caaccgggcg tgcagttcta
     2581 caccggaaac ttcctcgacg gaacggtcac gggccgggcc gggcgcccat acgcgcgtca
     2641 cgcgggcgtg tgcctcgaga cgcagcactt tccggacgcg ccgaaccgac ctgagttccc
     2701 gtcgatcgtg ctgcggccgg gtgaacgcta tgcgaccgcg acggtgttgc ggttcgatgt
     2761 cttaccctga cgctgcacga tctcccacag aggtttttcg ctccgcgctg cacattgtcg
     2821 gtttcgacgc cgatccgcga gcgacatgac ggccacgact ccaggcgcca cgctgcaccc
     2881 acgtccgcaa ctgcaacgcg agcgctggat ctcgctcgac ggaccctggc gtttcgcgtt
     2941 cgatcccgag cggcgctggg accggccggc tgacatcgag gactggccgc tcacgatccg
     3001 cgtgccgttc tgtcccgaga gcgggatgag cggcatcggc gacacgggct tccacccggt
     3061 ctgctggtac gagcgcgact tcgacctgcc gcaactgccg ccgaacggac gcctgctgct
     3121 gcatttcggc gcggtcgact accaggcccg cgtctgggtc aacgattacc ccgtcatgga
     3181 acacgaaggc ggccacgtgc ccttcgtgat cgacatcacc cgcgcaatcc ggcccggcgg
     3241 cacgcaacgg gtgaccgtgc aggcgctcga cgacccagag gatctggaga agcctcgcgg
     3301 caagcaggac tggaaactcg agccccacgg catctggtat ccgcgcacga gtggcatctg
     3361 gcagacggtc tggctcgagg cggtgtcgga gacgtatctg cgcgcggtca agtggacgcc
     3421 gaacctcgag cgctgggagg tgacgttcca cgcctacatc gacggcatgc aagcagacga
     3481 gaccgaggtg cggctgcggc tgcgggcggg cgacaagctg ttgatcgacg acgactaccg
     3541 cgtcgtgcac ggcgaggtcc accgtaagct ggggctctcc gacccgggca tcgacgactt
     3601 ccgcaacgag atcctctggt cgcccgagcg tccgcgactg ctgcaggcgg agatcgagct
     3661 cctccgggac ggcgcggtga tcgatcgggc gacgtcgtac acggcgctgc gcagcttcgg
     3721 cgtgcagcgc gaccggctga tgctgaacgg ccggcactac cagctcaggc tggtgctcga
     3781 ccagggttac tgg
//
LOCUS       DASPFR010000249         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1659967, whole genome shotgun sequence.
ACCESSION   DASPFR010000249 DASPFR010000000
VERSION     DASPFR010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659967"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..596
                     /locus_tag="VD737_06605"
     CDS             90..596
                     /locus_tag="VD737_06605"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HXW10266.1"
                     /translation="MPKAAGFTVIELLFVVALLAVLLSIGAPALQTMVTNNRISTAAG
                     DLMADLMFARATAISRSQRVGVCVSSDHATCSGSSWAQGWIVFMDANSNGNLDTGETI
                     VRVHEELAGALTVTPTPSALGIVFRPSGPADASRTFQICKAGFKGRTIAINATGRPQS
                     TAMASNCT"
     gene            601..1173
                     /gene="pilV"
                     /locus_tag="VD737_06610"
     CDS             601..1173
                     /gene="pilV"
                     /locus_tag="VD737_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HXW10267.1"
                     /translation="MRYLTYPALSRGFTMLEALISLLILSFGLLGIAGLYIAGVQNNK
                     SASLRTIATQQSYDMADRMRANLAGLTVPLPAMPFYHLPTAAQKANCYTTTNCSAQDM
                     AENDYFEWNDANSPSSNRRMLPGGFGITCLDSTPNDGTWNGTAVTHACDGLGTVYAIK
                     VWWLDDRRSEIATTDPNAYLRVVTTLQPQP"
     gene            1170..2012
                     /locus_tag="VD737_06615"
     CDS             1170..2012
                     /locus_tag="VD737_06615"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HXW10268.1"
                     /translation="MKNSERASFCRGRQHGMSLVELLVAMTIGLIITVVIANLFLGTK
                     QTYRTQDDLSRMQENLRFAFQVIGRTVRLAGYRADWNRTVDQVFLVNPPIDGSNGAGA
                     NGSDIITVRFQGSGTGASSANCVASNSCGGADGRVLDCLGNRVDRQMGPGSGVGISFV
                     ENRFSVRANGANGSPALFCSIDQGTTWVEIVPDIENMQVLYGEKTGPTAVDRYLAAGT
                     VGLNMAQVLNLRIALLHRSANLTAGETDTRTYDLVGTSVGPFNDQRIRLAATSNFVLR
                     NRTQ"
     gene            2027..2659
                     /locus_tag="VD737_06620"
     CDS             2027..2659
                     /locus_tag="VD737_06620"
                     /inference="COORDINATES: protein motif:HMM:NF025061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HXW10269.1"
                     /translation="MKHAITPRAQRGAILIISLMFLVVLTMLALTTMGGITLEERMSG
                     QYRDLNMAFQAAESGLRDAERDIWGAGTATATPRAPTISGRTGFGDGNDTANGTCTNN
                     SNTTYGWGLCFASAVGPHPPFLNVDLSPTSTIAVPYGKYTGATPLQYVSAQPRYIIEA
                     IWKDISPTPTLQSLSPVSGITTYYRITSQGFGVNPNTRVTLQEMYLKPWN"
     gene            2673..>3323
                     /locus_tag="VD737_06625"
     CDS             2673..>3323
                     /locus_tag="VD737_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10270.1"
                     /translation="MKLPLRKVLSATLAFCVATQPAQAALLTLATQPIYLGAAIPPQV
                     MITMTKDQQLFKKAYNDYTDLDNDGVLELTYKHSINYYGYFDSFKCYNYNTTNLRFEP
                     FATTADKYCTGANAGKWSGNFLNWASMTRMDAVRKLLYGGLRSEDKNDETVLERAYLP
                     TDAHSFAKYYNGADTNRLTPFTGLTTEQTYTSTTTMTLPNLVKIVSLTRNNTGASNP"
BASE COUNT          640 a         1180 c          987 g          516 t
ORIGIN      
        1 cgccgcgttt ccgcccgccg gtcgcgtcct tttgcggcgc ctgccgaccg ctccttagca
       61 tttcatcccg agaagataag ggaggctgca tgcccaaagc agcgggcttt acggtcatcg
      121 agttgttgtt cgtcgtggcg ctgctcgcgg tgctgctgag catcggcgcg ccggcgttgc
      181 agaccatggt gacgaacaac cgtatctcga cggccgcggg cgacctgatg gcggacctca
      241 tgttcgcgcg tgccaccgcc atcagccgtt cccagcgcgt cggcgtatgc gtgagctccg
      301 accacgccac ctgcagcggc agctcctggg cccagggatg gatcgtgttc atggacgcca
      361 acagcaacgg caacctcgac accggcgaga cgatcgtgcg ggtgcacgag gagctggccg
      421 gggcgctcac cgtgaccccg acgccgagcg cgctcggcat cgtgttccgt ccctccggcc
      481 cggcggacgc gtcgcgcacc ttccagatct gcaaggccgg attcaagggc cgcacgatcg
      541 cgatcaacgc gaccgggcgg ccgcaatcga ccgcgatggc ttcgaactgc acctgaactc
      601 atgcgctacc tcacataccc cgcacttagc cgcggcttca cgatgctcga ggcgctgatc
      661 tcgctgctca tcctgtcgtt cgggctgctc ggcatcgccg gcctctacat cgccggcgtg
      721 cagaacaaca agagcgccag cctgcgcacg attgcgacgc agcagagcta cgacatggct
      781 gaccggatgc gcgcgaacct cgccggcctg acggtgccgc tgccggcgat gccgttctac
      841 cacctgccga ccgcggcgca aaaggcgaac tgctacacca ccaccaattg ctcggcgcag
      901 gacatggcgg agaacgacta tttcgagtgg aacgacgcga acagcccctc gtcgaaccgc
      961 cgaatgctgc ccggcgggtt cggcatcact tgcctcgact ccacgccgaa cgacggcacc
     1021 tggaacggca ccgcagtgac ccatgcctgc gacggcctgg gcacggtcta cgccatcaag
     1081 gtgtggtggc tcgacgatcg ccgcagcgaa atcgcgacca cggatcccaa cgcctacctt
     1141 cgcgtcgtca cgacgctcca gccccagcca tgaaaaacag cgagcgcgca tctttctgca
     1201 ggggccgaca gcacggcatg tcgctggtcg agctgctggt cgcgatgacc atcgggctga
     1261 tcatcaccgt ggtaatcgcg aatttattcc tcggcacgaa acagacttac cgcacccagg
     1321 acgatctttc gcgcatgcag gaaaacctgc gcttcgcctt ccaggttatc ggtcgcaccg
     1381 tacgcctggc gggctaccgc gccgactgga accggaccgt cgaccaggtg ttcctcgtga
     1441 acccgccgat cgatgggagc aacggagccg gcgcaaacgg cagcgacatc atcaccgtgc
     1501 gcttccaggg tagcggcacc ggcgcgagca gcgccaactg cgtcgcgagc aacagctgcg
     1561 gcggcgccga cgggcgcgtg cttgactgcc tcggaaaccg cgtcgacagg cagatgggac
     1621 ccggaagcgg cgtcggcatc tcgttcgtcg agaaccgctt cagcgtgcgc gcgaacggtg
     1681 ccaatggcag ccccgcgctc ttctgcagca tcgatcaggg cacgacgtgg gtcgagatcg
     1741 tgcccgacat cgagaacatg caggtgctct atggcgagaa gaccgggccc accgcggtgg
     1801 accgctacct cgccgccggc accgtcggcc tcaacatggc gcaggtgctg aacctgcgca
     1861 tcgcgctact gcaccgctct gccaacctca ccgcgggcga gaccgacacc cgaacctatg
     1921 acctggtcgg caccagcgtg gggccgttca acgaccaacg catccgcctc gctgcgacca
     1981 gcaacttcgt gctgcgcaac cgcacgcaat gagagctgcc cgccccatga aacatgccat
     2041 caccccgcgc gcgcagcgcg gcgccatcct catcatctcg ctcatgttcc tggtggtgct
     2101 gaccatgctc gcgctcacca ccatgggcgg catcacgctc gaggagcgga tgtccgggca
     2161 gtaccgcgac ctgaacatgg cattccaggc cgcggagtcc gggctgcgcg acgccgagcg
     2221 cgacatctgg ggcgccggca ccgccacggc gacgccgcgc gcgccgacga tctcgggccg
     2281 cacgggcttc ggcgacggca acgacacggc gaacggcacc tgcaccaaca acagcaatac
     2341 gacgtacggc tggggcctgt gcttcgcgtc tgccgtgggc ccgcacccgc ccttcctgaa
     2401 cgtggatctg agcccgactt cgacgatcgc ggttccttac ggcaagtaca ccggggcgac
     2461 cccgcttcag tacgtgtcgg cgcagccgcg ctacatcatc gaggcgatct ggaaggacat
     2521 ctcgccgacg ccgacattgc agtcgctcag cccggtgtcc ggcatcacca cctattaccg
     2581 catcacctcg cagggcttcg gcgtgaatcc gaacacgcgc gtaaccctgc aggaaatgta
     2641 tctcaagcct tggaactagc cgaggaactg ccatgaagct cccgctcagg aaagtcctgt
     2701 ccgccaccct cgcgttctgc gtcgccacgc agcccgcgca ggcggcgctg ctcacgctcg
     2761 cgacgcagcc gatctacctc ggcgccgcga tcccgccgca ggtcatgatc accatgacca
     2821 aggaccagca gcttttcaag aaggcctaca acgactatac cgacctggac aacgatggcg
     2881 tgctcgagct gacgtacaag cacagcatta attactacgg gtatttcgac agcttcaagt
     2941 gctacaacta caacacgacc aacctgcgct tcgagccgtt cgccacgacc gctgacaagt
     3001 attgcaccgg cgccaacgcc ggcaagtgga gcggcaactt cctgaactgg gcgtctatga
     3061 cgcggatgga cgcggtgcgc aagcttctct acggcggtct gcgctcggaa gacaagaacg
     3121 acgagaccgt gctcgagcgc gcctacctgc cgaccgatgc gcactcgttc gccaagtact
     3181 acaacggagc cgataccaac cggcttacgc cgtttaccgg gctgacgacg gagcagacct
     3241 acaccagcac caccaccatg acgcttccca acctggtgaa gatcgtcagc ctcacccgga
     3301 acaacacggg cgcgagcaac cca
//
LOCUS       DASPFR010000250         3266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_560226, whole genome shotgun sequence.
ACCESSION   DASPFR010000250 DASPFR010000000
VERSION     DASPFR010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560226"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VD737_06630"
     CDS             <1..630
                     /locus_tag="VD737_06630"
                     /inference="COORDINATES: protein motif:HMM:NF021389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HXW10271.1"
                     /translation="TWVGRPIELPGSKPLRFEPLDEPGLALRDWPAHHVVKCLAFYHP
                     DDPGDLRRDQEARLEALHADCVALDRELLLELITTSSGRPNDDRTTARALTRLYNLGI
                     APAWWKLEPQTPASWHAIGQVIASRDPWCNGVLLLGLDAPEPDLVRSIADAAATPICR
                     GFAVGRSIFGAAARDWFAGTLDDDRAVATVAGNYTRLIGAWRGARGAVA"
     gene            627..2471
                     /gene="iolD"
                     /locus_tag="VD737_06635"
     CDS             627..2471
                     /gene="iolD"
                     /locus_tag="VD737_06635"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR04377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HXW10272.1"
                     /translation="MTTVRMTTAQAIVRYLMAQRIVVDGETVPFVQGVFAIFGHGNVA
                     GLGEALAAVRDRFPTYRANNEQAMAHAAVAYARAHARRRILACTTSIGPGATNMVTAA
                     AVAHVNRLPVLLLPGDVFASRRPDPVLQQVEDFGDGTVSANDCLRPVSRYFDRIVRPE
                     QLLRALPQAMSVLTDPAECGPVTLALPQDVQAEAWEFPERFFDTRLLEMPRLSADPER
                     FARAVEALRRARRPLAIAGGGVHYSGACAALAQFASRFGVPVTETQAGKGALPYDHPW
                     NAGAIGVTGTDAANRLAGEADLLLLVGTRLQDFTTGSGALFRDADRPVISINAGRHDA
                     AKYGAIALQADALRTLEDISTALERSPARAPDAAWQRRCEALRKSWFESAARAVADRG
                     TDRPSDAEVLGAVNDWLGPAGTMVCAAGGLPGELHKLWCASGPGHYHVEYGYSCMGYE
                     IAGGLGVKLARPDREVVVLVGDGSYLMMNSEIATSIALGLKLVIVVLDNRGFGCIHRL
                     QQSCGVEPHGNLFGDPASPDAPHVDFAAHARALGATAEKVSGIATLREALGRARAAPH
                     TAVVVIDTDPTRSTSDGGTWWDVPVAETSTSESVREARRRYESERGGS"
     gene            2468..>3266
                     /gene="iolE"
                     /locus_tag="VD737_06640"
     CDS             2468..>3266
                     /gene="iolE"
                     /locus_tag="VD737_06640"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR04379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HXW10273.1"
                     /translation="MTQTVRLGINPLTWSNDDLPSLGAENTLEDCLREARAAGYDGVE
                     LGHKFPREPDALRAALAPHGLSLVSGWYSARLLERDADAEFEAMQPHLGLLAALGSEV
                     MVVAEVTHCVHGDRHARQSRRPWLSPADWPRFNDRLTRLAELMQRRGVRLAYHHHMGT
                     VVQSADDVDRLMGSTGESVGLLLDTGHLRFAGADPVQVATRHARRIAHVHCKDVRADV
                     LADVRNRDTSFLDAVLAGVFTVPGDGSVDYDAVLAPLAAAGYRGWLVV"
BASE COUNT          461 a         1136 c         1188 g          481 t
ORIGIN      
        1 acctgggtcg gccgccccat tgaattgccg ggatcgaagc cgctgcgatt cgagccgctc
       61 gacgaacccg gcctcgcact gcgcgactgg ccggcacacc acgtcgtgaa gtgcctggcg
      121 ttctaccacc cggacgatcc cggtgatttg cggcgggacc aggaagcacg tcttgaggcc
      181 ctgcacgcgg attgcgtcgc gctcgaccgc gaactgctgc tcgagttgat cacgacgtcg
      241 agtgggcgcc cgaacgacga ccgcacgacg gcccgggccc tgacgcgcct ctacaacctc
      301 ggcatcgcgc ccgcatggtg gaagctcgag ccgcagaccc cggcgtcctg gcacgccatc
      361 ggccaggtga tcgcgtcgcg ggacccgtgg tgcaacgggg tgctgctgct cggactggac
      421 gccccggagc cggacctcgt gcgctcgatt gccgatgcgg cggcgacgcc gatctgtcgc
      481 gggtttgcgg tcggccgcag catcttcggc gcggcggcgc gcgactggtt cgcaggcacg
      541 ctcgacgacg accgggcggt ggcgaccgtg gccgggaatt acacgaggct gatcggcgcg
      601 tggcgcggcg cgcgtggagc cgtcgcatga cgaccgtacg aatgacgacc gcgcaggcga
      661 tcgtgcgtta cctgatggcg cagcgcatcg tcgtggacgg cgagaccgtg ccgttcgtgc
      721 agggggtctt tgcgatcttc ggccacggca acgtcgccgg actcggcgag gcgctcgcgg
      781 cggtccgcga ccggtttccg acgtaccgtg caaacaacga acaggccatg gcgcacgcgg
      841 ccgtggccta cgcccgggcg cacgcgcgcc gccgcatcct cgcctgcacg acctcgatcg
      901 gccccggcgc gaccaacatg gtcacggccg ccgccgtggc gcacgtcaac cggctgcccg
      961 tgctgctgct gccgggcgac gtgttcgcga gccggcggcc ggacccggtg ctgcagcagg
     1021 tcgaggactt cggcgacggg accgtgtcgg ccaacgactg cctgcggccg gtctcgcgct
     1081 acttcgaccg catcgtgcgg ccggaacagt tgctgcgggc actgccgcag gcgatgtcgg
     1141 tgctcacgga ccccgccgag tgcggaccgg tcacgctggc gttgccgcag gacgtgcagg
     1201 ccgaggcatg ggagttcccg gaacgattct tcgacacgcg gctgctcgag atgccgcgcc
     1261 tgagtgccga tccggagcgg tttgcgcgcg ccgtcgaagc gctgcgtcgc gccaggcgtc
     1321 cactcgcgat cgccggcgga ggcgtgcact actcgggcgc gtgcgcggca ctcgcgcagt
     1381 ttgcgtcgcg tttcggtgtg cccgtgaccg agacgcaggc cggcaagggc gcccttcctt
     1441 acgatcaccc gtggaacgcc ggcgccatcg gcgtgacagg cacggacgcc gccaaccgcc
     1501 tggccggtga ggcggacctg ctgctgctcg tcggcacgcg actgcaggac ttcacgaccg
     1561 gctctggcgc gctgtttcgc gatgcggacc ggcctgtgat atcgatcaac gccggccgcc
     1621 acgatgcggc gaagtacggc gcgatcgctt tgcaggcgga cgcgctgcgc acgctcgagg
     1681 acatctcgac ggctctcgaa cgttcgccgg cccgagcgcc ggacgccgcg tggcagcgcc
     1741 ggtgcgaagc gctccgcaag agctggttcg agtcggccgc ccgcgctgtc gcggaccgcg
     1801 gcacggaccg gccatcggac gccgaggtgc tcggggcggt caacgactgg ctcggtcccg
     1861 cgggcacgat ggtctgtgcg gccggcgggc tgcccggtga gctgcacaag ctctggtgcg
     1921 cctcggggcc cggccactac cacgtcgagt acggctactc gtgcatgggc tacgagatcg
     1981 ccggcggcct cggcgtcaag cttgcgcgtc ccgaccgcga ggtcgtcgtg ctggtgggcg
     2041 atggcagcta cctgatgatg aattcggaga ttgcaacgtc gatcgcgctc ggcctgaaac
     2101 ttgtgatcgt cgtgctcgac aatcgtggct tcggttgcat ccaccggctc cagcagtcct
     2161 gtggcgtcga gccgcacggc aacctgttcg gcgatccggc gagcccggac gcgccgcacg
     2221 tcgacttcgc ggcgcacgcc cgcgccctcg gcgccacggc cgagaaggtt tccggcatcg
     2281 cgaccttgcg cgaagcactc gggcgcgccc gcgcggcgcc gcacaccgcg gtcgtcgtga
     2341 tcgacacgga ccccacgcgc tcgacgagcg acggcggcac ctggtgggac gtgcccgtgg
     2401 cggaaacgtc gacctcggag tcggtgcgcg aagcacggcg ccgctacgag agcgagcgag
     2461 gcgggtcgtg acgcagacgg tgcggctcgg catcaacccg ctcacttgga gcaacgacga
     2521 cctgccgtcg ctcggcgccg agaatacgct cgaagactgc ctgcgcgagg cgcgcgccgc
     2581 ggggtacgac ggggttgagc tcgggcacaa gtttccgcgt gagcccgacg cgctgcgtgc
     2641 ggcgctggca ccgcacgggt tgtcgctggt gtccggctgg tacagcgcgc ggctgctcga
     2701 acgcgacgcg gacgccgagt tcgaagcgat gcaaccgcac ctcggactgc tcgcggcgct
     2761 cggcagcgag gtgatggtcg tcgcggaagt gacgcactgc gtgcacggcg accgtcacgc
     2821 acggcaatcg aggcggccct ggctctcacc cgccgactgg ccgcgcttca acgaccggct
     2881 cacgcggctg gcggaactca tgcagcgtcg cggcgtgcgc ctcgcgtatc accaccacat
     2941 gggcacggtg gtgcagagtg cggacgacgt cgatcgcttg atgggctcga cgggcgagtc
     3001 ggtcggcctg ttgctcgaca cggggcacct gcgatttgcc ggcgccgatc cggtccaggt
     3061 ggcgacgcgg cacgcgcgca ggatcgcgca cgtgcactgc aaggacgttc gagccgacgt
     3121 cctggccgac gtgcgcaacc gcgacacgag cttcctcgat gcagtgctcg ccggagtgtt
     3181 caccgtgcca ggggacggat cggtcgacta cgacgccgtg ctcgcgccgc tcgcggccgc
     3241 ggggtaccgc ggatggctgg tcgtcg
//
LOCUS       DASPFR010000251         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_73032, whole genome shotgun sequence.
ACCESSION   DASPFR010000251 DASPFR010000000
VERSION     DASPFR010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73032"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..436)
                     /locus_tag="VD737_06645"
     CDS             complement(68..436)
                     /locus_tag="VD737_06645"
                     /inference="COORDINATES: protein motif:HMM:NF019473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXW10274.1"
                     /translation="MSAMQDTLRSIMEATRANDDERFLAHLADDVEYVFHVGTRGVQG
                     KDGVRRFLAKYRTIAKDVVWRVDRVAESDDCLFIEGYEEYTDQRTGERIAHPYMGIME
                     FRDGKVAKWRDYFEMNQTKK"
     gene            complement(470..1669)
                     /locus_tag="VD737_06650"
     CDS             complement(470..1669)
                     /locus_tag="VD737_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXW10275.1"
                     /translation="MTHHATAIKPALDFDGLRPPSPWLTDVHREWRDRVRAFVVDEIE
                     PHLDAWDAAGTFPDSLYVTAARRGMLGVGFPPELGGHTADADPWYRMLFAEELHRPGS
                     GVVFADLATHWIALPPVLQFGGPDLQESVVRPVLAGERRMAFAVTEPGGGSDAGALQT
                     RAERRGDAWVVNGSKTLISGAMRADDILTAVRTGGPGAGGISLLLVDASSPGIERRAV
                     PGLRWYNSSIGTIEFNDVHVPVARLIGPQDRGFGALAKQFNVERLSGIAATLAMSRTA
                     VAEAIDWAQRREAFGRRLVEHQALRHKLVDLVRAIRASYAFLDQCVWRLQRGETVIAD
                     IAMLKVQATRTLERCAREAMHVLGGRAYAGPTRVERIYREARIFALGGGTEEVLDDQA
                     ARQLGLL"
     gene            1764..>2150
                     /locus_tag="VD737_06655"
     CDS             1764..>2150
                     /locus_tag="VD737_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10276.1"
                     /translation="MNTSPRPATIVRLPQSLRVPVVAAPMFLISGPELVIEACRAGIV
                     GAFPTVNARTVEDLDAWMAQITATLGSPAAAGAAPWAANLITHSTNQRLSQDLELVAR
                     YKPPIVITALGSPQPAIELVHSYGGIV"
BASE COUNT          324 a          758 c          713 g          355 t
ORIGIN      
        1 tagtcactat tcactattca ctaatcacta gtcactattc actagtcact tcccagtcac
       61 ttccccgtca cttcttcgtc tggttcatct cgaagtaatc ccgccacttg gccaccttgc
      121 cgtcgcgaaa ctccatgatg cccatgtaag ggtgcgcgat gcgttcgccg gtgcgctggt
      181 cggtgtattc ctcgtaaccc tcgatgaaca ggcagtcgtc gctctcggcg acgcggtcca
      241 cgcgccagac gacgtccttg gcgatcgtgc ggtacttcgc caggaagcgg cgcacgccgt
      301 ccttgccctg cacgccgcgg gtgccgacgt ggaagacgta ctcgacgtcg tccgcgaggt
      361 gggccaggaa gcgctcgtcg tcgttcgcgc gggtcgcttc catgatcgaa cgtagcgtgt
      421 cttgcatcgc ggacatcgtg tgtctcctgc tgtgttcgtg aatggacggt cagagcagcc
      481 cgagctgccg cgcggcctgg tcgtcgagca cttcctcggt gccgccgccc agcgcgaaga
      541 tgcgggcttc gcgatagatg cgttcgacgc gcgtggggcc ggcgtaggca cggccgccca
      601 gcacgtgcat tgcctcgcgt gcgcagcgct cgagcgtgcg cgtggcctgg accttgagca
      661 tcgcgatgtc ggcgatcacc gtctctccgc gctgcagccg ccagacgcac tggtcgagga
      721 acgcgtacga ggcacggatc gcgcggacga ggtccacgag cttgtgccgc agtgcctggt
      781 gctcgacgag cctgcgcccg aacgcttcgc gtcgctgtgc ccagtcgatc gcctcggcca
      841 ccgccgtgcg cgacatcgcg agcgtcgcgg cgatgccgct gagacgctcg acgttgaact
      901 gcttcgcgag cgcgccgaag ccacggtcct gcggcccgat cagccgcgcg accggcacgt
      961 gcacgtcgtt gaactcgatc gtgccgatgc tcgagttgta ccagcgcagc cccggcacgg
     1021 cccgccgctc gatgcccggc gatgacgcgt cgacgagcag cagcgaaatg ccgcccgccc
     1081 cgggaccgcc ggtgcgtacg gccgtcagga tgtcgtcggc acgcatggcg cccgagatca
     1141 gggtcttgga gccgttgacg acccacgcgt cgccgcgccg ctccgcgcgc gtctgcaacg
     1201 cgcccgcgtc cgagccgcca ccgggctcgg tgacggcgaa cgccatccgg cgctcgcccg
     1261 ccagcacggg tcggacgacc gactcctgca gatcagggcc gccgaactgc aggaccggcg
     1321 gcagcgcgat ccagtgcgtc gcgaggtcgg caaacaccac gccactgcca gggcgatgca
     1381 gctcctcggc gaacaacatc cggtaccacg gatcggcgtc cgcggtgtga ccgcccagct
     1441 cgggcgggaa cccgacgccg agcatgccgc gccgggcggc ggtcacgtag agcgagtccg
     1501 gaaaggtgcc ggctgcgtcc caggcgtcga gatgcggctc gatctcgtcg accacgaacg
     1561 cccgcacgcg gtcgcgccac tcccgatgca cgtccgtgag ccagggcgaa ggcgggcgca
     1621 ggccgtcgaa gtcgagcgct ggcttgatcg cggtggcgtg gtgcgtcatc gatcctgcct
     1681 cctgcacggc gcgccgccgt ctcgattgac ggtcgtcgag tgcctgggat aaagtcagta
     1741 cagtttagca agtgacgagt gtcttgaaca cgagcccgcg cccggccacg atcgtgcgac
     1801 tgccgcagtc cctccgcgtg cccgtggtcg cggcgccgat gttcctgatc tcgggacccg
     1861 agctcgtgat cgaggcttgt cgggccggca tcgtgggcgc ttttccgacc gtcaacgcgc
     1921 gcacggtcga ggacctcgac gcctggatgg cgcagatcac ggccacgctg gggtccccgg
     1981 ccgcggcggg cgcggcaccc tgggccgcga acctgatcac gcactcgacc aaccagcgct
     2041 tgtcgcagga cctcgagctc gtcgcgcgtt acaagccacc gatcgtgatc accgcgctcg
     2101 gcagtccgca gcccgccatc gaactggtgc attcgtatgg cggcatcgtg
//
LOCUS       DASPFR010000252        15863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_805520, whole genome shotgun sequence.
ACCESSION   DASPFR010000252 DASPFR010000000
VERSION     DASPFR010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15863
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805520"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="VD737_06660"
     CDS             complement(<1..1421)
                     /locus_tag="VD737_06660"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXW10277.1"
                     /translation="MCTIDTRADARRPFALRLPNGSLALVCAVALSVAACASPGPARD
                     DGRDGRRDHQASESPRERPLREFAAQPYPSTYQPLPRRDTLIRGATVLDGAGRRLERA
                     DVLLRDGRVAAVGTGLEAPPGVEIVEAAGRWVTPGIVDPHSHLGNFPTPYTAEDAQHT
                     DVNEDTDPNTAQVWAQHSINVQDPGFTRALAAGVTTLQVMPGSTNLFGGRTVVLRTVP
                     ASTVQGMMYPGAPFGLKMACGENVVHAYGDSGGFPVSRMGAVSGQRAAWIAARKYLHD
                     WRRYASGEEHRPPEPDLELDTLAGVLTGDIRVHAHCYRADDIAWLFGMAREFEFSITA
                     IHHATEAYKIPELFVREGACAAVWSDWWGYKREAYDAIRENAAFLDAAGACVAMHSDS
                     AIVGQRLNLEAAKAMAAGARAGVRVAPERAIEWITRNPARMIGLGDAIGSLEPGKVAD
                     VVVWSGDPFSVYTHADRVYVDGAL"
     gene            complement(1533..2165)
                     /locus_tag="VD737_06665"
     CDS             complement(1533..2165)
                     /locus_tag="VD737_06665"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HXW10278.1"
                     /translation="MSSVSGVFAPPSERDVTRLVLEHPLAWLVTSGGDEYAATPLPLR
                     PVVGEQGEVVELLGHFARSNPHHELARRVRPTLVLFSGPHGYVSPSWMRDRTRFPTWN
                     YAYVQYLVDIRYTEDPAETQEILRDLVGAMERDRPDAWAIEDGGARYDKLVHGIIAFR
                     ARILERRVKFKLAQDERAGEYADIVQALEAAGQRELLEWTRRSNAARTRR"
     gene            2356..2802
                     /locus_tag="VD737_06670"
     CDS             2356..2802
                     /locus_tag="VD737_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10279.1"
                     /translation="MRFPARPALLACLAGCTALLGTSVSLADPPAPRKLVEIYRIAPG
                     KHVEFLRMIAKFDAASKDAGIPPRELYVHQDGGNWDFMLIQDAEYTDEQRAKIGPVME
                     KMGLPRGADFFVEFRQFVAEHSDTFVEGPTTAEAWLGKLSPSAGKP"
     gene            complement(2799..4232)
                     /locus_tag="VD737_06675"
     CDS             complement(2799..4232)
                     /locus_tag="VD737_06675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HXW10280.1"
                     /translation="MRREFRRPISGCTAALAVVCAVMLAGCALTREPVDTPQLPLPEA
                     LPAVEGAAAELPSPWWALFADDTLDALVREALEYNADLQAATARVAEARALAGISRAD
                     RLPGLDLEAGVTRGRDSALANPGLPGVDATRTDYGVRGVVSYEVDLWGRYAHASEAAR
                     LRLLATEFDRDALRLSLTGETARAYFALAAAIAQFEQARATLASREESLRIERLRFEG
                     GESDEITWRRVEAEAEQARALMREFEFAVGQRQNVLGVLTGRTPRDLTGQRILPRAMP
                     VQTTVPRLPAGLPADVLVRRPDVRAAEAQIAAAAGDVGAARAALLPGISLTGAYGSAS
                     TELGDLFTNPADVWSVTGALLQPIFQGGRLRSNVARTRALQQQRQAEYAGTVRRAFRE
                     VLDGLQGQDSLRAVEAARAVQVASLRRATELAELRYAEGEIAYLELLDVRRGLFAAEI
                     ELVAARRAALANTVDLALALGGGAPLP"
     gene            complement(4222..7365)
                     /locus_tag="VD737_06680"
     CDS             complement(4222..7365)
                     /locus_tag="VD737_06680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HXW10281.1"
                     /translation="MISSYFIDRPIFASVISAFIVIAGLAALRVLPVAQYPDILPPQV
                     EVQAFYPGANAETVAQTVAAPLDQAVNGVDGMLYVRSASAGNGALSMAVTFAIGTDPD
                     LNAINVNNRVQSALSTLPEEVRRQGVTVRKSQQSFLKVIALDSDDPRYDALFISNYAL
                     LNIVDELRRIPGVGDAQIFGSKDWSIRIWLRPDVLAKLGLTPGDVADAVREQNAQFAA
                     GRIGQEPMDDRVDFTFSVTTQGRLQDPEDFGRIVVSTSESGGIVRLEDVARVELGSRE
                     YDFSATRNGKASVPIGIFLQPGANALEVSDAIDARMEELEARFPAGLEYSYPYDTTTY
                     IKVSIREVLVTLLEAMALVFLVVFVFLQNWRATVIPMLAVPVSLIGTLAAMLAFGFSI
                     NTLTLFGMVLSIGIVVDDAIVVLENVERIIATEGLDPRAATHKAMQQVTRPVIAIVFV
                     LTAVFLPVAFLGGLVGEMYRQFAVTIAVSVAISGFVALTLTPALCASLLRQEDAVSHG
                     LLERFHDWFARMTGRYANGVRYLLRHTALALGLVAAMLVATIALARAVPNALAPKEDQ
                     GYLMVIPILPDAASLQRTEAVSEQITEALLANPYVDQVMTFSGLDALTFTLRTNTGIT
                     WVSFKDWSERTAPDASASAIAGYVFGAGAAIKDAFVLAFEPPPIEGLSMTGGFDGYVQ
                     SRGGGSMKELEAAAQSLVAAASQRKELVNVTTTFSASVPQVRIDVDREQAKLLGIDVA
                     EVFETLQSTFGALYVNDFNRAGRVFRVHLQSEAEFRAHPDDIRDVYVRAGDGQMIPLT
                     ALVTIRESTGPEVIERYNVFPSAKVLGEAAPGYSSGEALAVMEEVARTALPDGYALEW
                     TGTSFQEKVSGGTSNVVFLVGVLMVFLVLAAQYEKWTLPFAVILAVPFAVFGALLAVF
                     LRGQENDLYFQIGLVTLVGLASKNAILIVEFAQLKVQEGMSLADAAVEGARLRFRPIV
                     MTSLAFILGVLPLALSGGAGAASRHSIGTGVIGGMLAATFIATLFVPLFFKLVAARGV
                     DKFHAPRAVVEEGSDAA"
     gene            complement(7362..8513)
                     /locus_tag="VD737_06685"
     CDS             complement(7362..8513)
                     /locus_tag="VD737_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXW10282.1"
                     /translation="MTHTAGGPSRRPLALLLPLVALAAGCGQSQSQQGGEMPPPEVGV
                     VQVTRGSVPILLEFTGRAAGSREVEVRARVDGILLERRYREGSEVREGDVLFRIDPEP
                     YRAAVAQARAELGVAQARFEEARRQRDRIVPLFEQNATSQRQRDEAVSQFEVAQASVA
                     AARARLRTAELDLGYTDVRAPISGLTSREVRSEGSLVQAGSEASLLTRIVQVHPIYVE
                     FSVPEAEAARIRRSPEQLVVKLMLEGERADAVAGRLSFVDTSVEPGSGTVRVRAEFDN
                     ADRRIVPGQFVRVRVEGIAEPDAVAVPRKAVMSSAQGHFVWVVGADDTVQLQPVRLQG
                     DAAGLALIGEGLQGGERVVAEGVLKVRPGVKVKVATPEPPTGAAGGAPQ"
     gene            8699..9394
                     /locus_tag="VD737_06690"
     CDS             8699..9394
                     /locus_tag="VD737_06690"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXW10283.1"
                     /translation="MRTSTRSAVFAALASCTLPALAAEPAMDEAARMQYALGYQLGKD
                     LTAVESRPQDLARGVEDGRSGAKPKLSEAEMEAALGLLQQRVTEQRTKSQAEQAEKAR
                     ASGEAYLAANAKKPGVTTTPSGLQYRVVTQGTGKKPVASETVTVHYKGTLVDGTEFDS
                     SYKRGQPATFPVSGVIPGWTEALQLMPIGSKYELAIPPALAYGSQGPLANQVLLFEVE
                     LLGTTADADDAAK"
     gene            9412..10311
                     /locus_tag="VD737_06695"
     CDS             9412..10311
                     /locus_tag="VD737_06695"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXW10284.1"
                     /translation="MSIAQVDGVQIAYEVRGEAAAPAVLLVMGLGMPAAMWPDEFVQA
                     LLDRGFRVVTFDNRDSGASTRLSGLPVPNIPVAMTRALMGLRVAAPYTLTDMAIDAAG
                     VLDAAGIEAAHVVGVSMGGMIAQVLAALLPQRVLTLTSIMSSSGNPAPRVALGDARAT
                     RALLRRPPADPADGEAIVEHLVYVFGVIGSPGYPQDDAARRAHLRRVAGCGLYREGTM
                     RQLGAILASGDRRTMLRNVEAPTLVIHGRSDPLVPVAAGRDTARYVRGAQLEIIDGMG
                     HDFPPALMTRIAERVAAHCAVSA"
     gene            complement(10303..11772)
                     /locus_tag="VD737_06700"
     CDS             complement(10303..11772)
                     /locus_tag="VD737_06700"
                     /inference="COORDINATES: protein motif:HMM:NF023407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HXW10285.1"
                     /translation="MSTIQLALLSHTNVGKTTLARTLLRRDIGAVMDRAHVTETAESH
                     VLLRTDEGDELVLWDTPGFGDSVRLARRLAQSGQPLGWFLSQVWDRFADRPFWCSQQA
                     MRAARDSADVVLYVANATEDPDAAGYVAPELQILEWLGKPTLVLLNQLGTHSDAGREL
                     EVASRWQRLLDSAGVPGGRRVLPFDAFARCWLQEHTLLQAVVECLSDEVQPAFARLRE
                     AWAARDEQVLEQSAAVIARQMARVASDAEAVGEASLADKARGLFRGGGDVDPAEQGAQ
                     RALLARLDEAVREGTAELVALHGLTGGASEEILANLARPFETERAADADRAGLFGAIV
                     TGALSGLAADLAAGGLTFGAGAMLGGLAGAFGARKLTQLYNAERGVAGSTVRWSDEFL
                     TSRVEAAIVRYLAVAHYGRGRGEFRAGTADPRWQEVATRLVSARPGVAADLLAGLRNP
                     APGTDDAAAALHDLLWRALEVLYPEAAAHYRASRRAPSG"
     gene            complement(11769..13310)
                     /locus_tag="VD737_06705"
     CDS             complement(11769..13310)
                     /locus_tag="VD737_06705"
                     /inference="COORDINATES: protein motif:HMM:NF022518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HXW10286.1"
                     /translation="MHADALRRILLVKSVEDHDADGAVLTQGEREAATREAVRAHPAS
                     ETSAGRDARTWRVLDRRASELYQRLVQRHPVVGRTMMLESHASQASLLVVLVAFASGV
                     VLSLVDSRVRIEVLAFPLLGVVLWNLAVYLVLAIAALRRRPNGARATAGATAGWVAWP
                     LRWGWRRAAALVRQASFYHRPLAAALRRFADDWWPHAQPLMALQGKRVFHVAAAALAI
                     GLVAGLYVRGIALEYRAGWESTFLTPGQVRTLLHALYGPASAVTGIALPADDATIDAL
                     HWRGGSGGGPAAPWIHLSAATALLFVVLPRLLLAAWSWISLARLSRSLAPPDALLPYA
                     RGVLGASDAAPAAATVRVTPYAYRPDTSSLAGLNVLLQATHGPGTKLELGEPIAYGSE
                     DDLRSRPVTQGDVEAILFTLAATPEAENHGAVLVALRDALARARSAARLLVIVDETPF
                     VAHARGDASFDARVEERRGAWREFVARHGAEACIADLGVLGRVEDVPPELVGRLRASA
                     RPVLA"
     gene            13395..15281
                     /locus_tag="VD737_06710"
     CDS             13395..15281
                     /locus_tag="VD737_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXW10287.1"
                     /translation="MPSVFRKFESLVQTYPDARPATPPRAFFAFLWDCTRGMRPWIFA
                     MTFCGALIAAFEALLFAMLGRVVDWLAEVEPAQLWAQERASLLLLAAVIAASPLVVWA
                     FSLIKYQVLFANFPMRLRWNFHRLMLGQSMSFYQDEFAGRVATKVMQTALAARDAWMT
                     TMDILAFITIYFITMLSIVGTFDLWLLVPFVGWLVLYVSCLRFFVPRLGRVSAAQADA
                     RALMTGRVTDAYTNIATVKLFSHASREAGYVKASMQEFMQTAYAQMRLVSGFEIANHV
                     LSMLLIASTAGAALWLWIGGEVGIGAVAAATAMALRLNGISHWIMWEMASLFEHVGTV
                     QDGMRTLSKTHTVVDAPGAPPLVVSRGEIRFEHVTFGYGGHRAVVDDLTLHIRPGEKI
                     GLVGRSGAGKSTVVNLLLRFYDVEQGRILIDGQDIATVQQDSLRAQIGMVTQDTSLLH
                     RSVRDNIVYGRPDASEQDMVLAAQRAEAHDFVLGLVDAKGRRAYDAHVGERGVKLSGG
                     QRQRIAIARVMLKDAPILLLDEATSALDSEIEAAIQQSLYRLMEGKTVVAIAHRLSTI
                     AAMDRLIVMDKGRIVEEGDHRSLLARGGLYARLWAHQSGGFLGEETGDEDDVATAARA
                     AASG"
     gene            complement(15614..>15863)
                     /locus_tag="VD737_06715"
     CDS             complement(15614..>15863)
                     /locus_tag="VD737_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HXW10288.1"
                     /translation="GRVLLDAEMDAPVVDEVREVVASLPGAPRLLDLHVWRVGRGKYA
                     CIVSLGAEHDGLRAESVRQALSVHDELVHVTVEIERGR"
BASE COUNT         2343 a         5752 c         5545 g         2223 t
ORIGIN      
        1 agggcgccgt ccacgtacac gcggtccgcg tgcgtgtaga cgctgaacgg atcgccggac
       61 cacacgacca cgtccgcgac cttgccgggc tcgagcgagc cgatcgcgtc gccgagcccg
      121 atcatgcggg ccgggttgcg cgtgatccac tcgatcgcgc gttcgggcgc gacacgcacg
      181 ccggcgcggg cgcccgcggc catcgccttc gcggcctcga ggttcagtcg ctgtcccacg
      241 atcgccgaat ccgaatgcat cgccacgcac gcgcccgcgg cgtcgaggaa cgcggcgttc
      301 tcgcggatcg cgtcgtaggc ctcccgcttg tagccccacc agtccgacca gaccgccgcg
      361 caggcgccct cgcgcacgaa cagttctgga atcttgtagg cctcggtcgc gtggtggatt
      421 gccgtgatgc tgaactcgaa ctcgcgggcc atgccgaaca gccaggcgat gtcgtcggcg
      481 cggtagcagt gcgcgtgcac gcggatgtct cccgtcagga ctccggccag cgtgtcgagc
      541 tcgaggtccg gctccggcgg tcggtgctcc tcgccggaag cgtagcgacg ccagtcgtgc
      601 aggtacttgc gcgcggcgat ccaggccgcc cgctggcccg acacggcgcc catgcgactg
      661 accggaaagc cgccgctgtc gccgtatgcg tgcacgacgt tctcgccgca cgccatcttg
      721 aggccgaacg gcgcgcccgg atacatcatg ccctgcacgg tcgaggccgg caccgtgcgc
      781 agcacgacgg tgcgcccgcc gaacaggttg gtggaacccg gcatcacctg cagcgtcgtg
      841 accccggcgg cgagcgcccg cgtgaagccc ggatcctgaa cgttgatcga atgctgcgcc
      901 cagacctgcg ccgtgttcgg gtcggtgtcc tcgttgacgt cggtgtgctg cgcgtcctcg
      961 gcggtgtacg gcgtcgggaa gttgccgagg tgcgagtgtg gatcgacgat gccgggcgta
     1021 acccagcgac cggccgcctc gacgatctcg acgccgggcg gcgcttcgag gcccgtgccc
     1081 acggccgcga cgcggccgtc gcgcagcagc acgtcggcac gctcgagccg ccgcccggca
     1141 ccgtcgagca ccgtcgcacc gcggatcagc gtgtcgcgcc gcggcagcgg ctggtaggtg
     1201 ctggggtacg gctgcgcggc gaattcgcgc agcggtcgct cgcgcggcga ctcgctggcc
     1261 tggtggtcgc gacggccgtc acggccatcg tcgcgtgccg gccccgggct cgcgcaggcg
     1321 gcgaccgaga gcgccacggc gcacacgagc gcgagcgacc cgttcgggag gcgaagagcg
     1381 aagggccgcc gcgcgtcggc ccgggtgtcg atcgtgcaca tccgattctt cggcacctgc
     1441 agagctgatc cgatgctagc accgacccct gcgccgaccc tttcgtgcgg gccagtcgtt
     1501 cgtcgcccgc gggccacgcc cgagccgtga cttcagcggc gcgtacgcgc cgcgttgctg
     1561 cgccgcgtcc actcgagcag ttcgcgctgg cccgccgcct cgagcgcctg cacgatgtcg
     1621 gcgtactcgc cggcgcgctc gtcctgcgcg agcttgaact tgacccggcg ctcgaggatg
     1681 cgcgcccgga acgcgatgat gccgtgcacc agcttgtcgt agcgcgctcc gccatcctcg
     1741 atcgcccagg cgtccgggcg gtcgcgctcc atcgcgccca cgaggtcgcg caggatctcc
     1801 tgcgtctcgg ctgggtcctc ggtgtagcgg atgtcgacga ggtactgcac gtacgcgtag
     1861 ttccaggtcg gaaaccgcgt gcggtcgcgc atccacgacg gcgacacgta gccgtgcggc
     1921 ccgctgaaga gcaccagggt cggacgcacc cggcgcgcga gctcgtggtg cggattgctg
     1981 cgggcgaaat gcccgagcag ctcgaccacc tcgccctgtt cgccgacgac ggggcgcagc
     2041 ggcaacggcg tcgccgcgta ttcgtcgccg ccgctggtca ccagccaggc gagcgggtgc
     2101 tcgagcacga ggcgtgtcac gtcgcgctcg gacggcggcg caaagacgcc ggaaacgctg
     2161 ctcatgaggg ctctcggtgt gctgcgatgc tcggagccta gggtacgaaa gcgtcgcctg
     2221 ccaacgcgca cgggcaacga atcgacggga tcaggcgacc cgcggcggac tgccgcgacg
     2281 gttgttgtcc cgttcgagca agtccgcggg cacggcctcg gcctagagtc ggggaccgcc
     2341 actcaggaga tcacgatgcg attccccgcg cgccccgccc tgctcgcctg cctcgccggc
     2401 tgcactgccc tgctcggcac gagcgtgtcc ctcgccgacc cgccggcccc gcgcaagctc
     2461 gtcgagatct accggatcgc gccaggcaag cacgtggagt tcctgcgcat gatcgcgaag
     2521 ttcgacgcgg cctcgaagga cgccggcatc ccgccgcgcg agctctacgt gcaccaggac
     2581 ggcggcaact gggacttcat gctgatccag gacgccgagt acaccgacga gcagcgggcc
     2641 aagatcggcc ccgtgatgga gaagatgggc ctgccgcgcg gtgccgattt cttcgtcgag
     2701 ttcaggcagt tcgtcgcgga gcacagcgac acgttcgtcg aaggtcctac gaccgcggag
     2761 gcctggctcg ggaaactgtc gccgtcggcc ggcaagccct agggcagcgg cgcgccgccg
     2821 ccgagcgcca gggcgaggtc gaccgtgttc gcgagcgcgg cccggcgagc ggcgaccagc
     2881 tcgatctcgg cggcgaacag gccacgtcgc acgtccagca gttccaggta ggcgatctcg
     2941 ccctccgcgt agcgcaactc cgcgagctcg gtggcgcggc gcagcgatgc gacctgcacc
     3001 gcgcgcgcag cctcgaccgc gcgcaggctg tcctgcccct gcaggccgtc gagtacctcg
     3061 cggaaggcgc gccgtacggt cccggcgtat tccgcctggc gttgctgctg cagggcccgc
     3121 gtgcgcgcca cgttcgaccg gagcctgccg ccctggaaga tcggctgtag cagcgcgccc
     3181 gtcaccgacc acacgtccgc aggattcgtg aacaggtcgc cgagttccgt gctcgccgag
     3241 ccgtaggcgc cggtgagtga gatgcctggc agcagggccg ccctcgcggc cccgacgtcg
     3301 cctgccgcgg ccgcgatctg cgcttcggcg gcgcgcacgt cgggccgtcg cacgagcacg
     3361 tcggcgggta acccggccgg cagccgtggc acggtcgtct gcaccggcat cgcacggggc
     3421 aggatgcgct ggcccgtgag gtcgcgcggc gtgcgaccgg tcagcactcc cagcacgttc
     3481 tgccgctgcc cgaccgcgaa ctcgaactcg cgcatcaacg cgcgcgcctg ctcggcctcc
     3541 gcctcgacac ggcgccaggt gatctcgtca ctctcgccgc cctcgaaccg cagccgctcg
     3601 atccgcagtg attcctcgcg cgaggcgagc gtcgcgcgcg cctgctcgaa ctgcgcgatc
     3661 gccgccgcca gcgcaaaata cgcgcgcgcc gtctcgccgg tcaggctcag gcgcagcgcg
     3721 tcgcggtcga actccgtagc gagcaatcgc aggcgcgcag cctccgaggc gtgcgcgtac
     3781 cggccccaca ggtcgacctc gtacgaaacg acgccccgca ccccgtagtc ggtgcgcgtc
     3841 gcgtcgacgc ccggcaggcc gggattcgcc agcgcgctgt cgcgcccgcg cgtgacgccg
     3901 gcctcgaggt cgagcccggg caggcggtcg gctctcgaga tgccggcgag cgcacgggct
     3961 tccgcgacgc gggcggtcgc cgcctgcagg tcggcgttgt actcgagcgc ctcgcgcacg
     4021 agcgcgtcga gcgtgtcgtc ggcgaacagc gcccaccacg ggctcggcag ctccgcggcc
     4081 gcgccctcga cggcaggcaa cgcctcgggc agcggcagtt gcggcgtgtc gaccggctcg
     4141 cgcgtcagcg cgcagccggc cagcatcacg gcgcacacga ccgcgagtgc ggccgtgcag
     4201 cccgaaatcg gccgccgaaa ctcacgccgc atcgctgcct tcctcgacga cggcgcgcgg
     4261 agcgtggaac ttgtccacgc cccgggcggc gaccagcttg aagaacaaag gcacgaacag
     4321 cgtcgcgatg aacgtggcgg ccagcatgcc gccgatgacg ccggtcccga tcgagtggcg
     4381 gctcgcggcg ccggcgccgc cgctgagcgc gagcggcagc acgcccagga tgaacgcgag
     4441 cgaggtcatc acgatcgggc ggaagcgcag cctggcgcct tcgacggccg cgtcggcgag
     4501 cgacatgcct tcctggacct tcagctgcgc gaactcgacg atcaggatcg cgttcttcga
     4561 ggcgaggccg accagcgtga ccagcccgat ctggaagtag aggtcgttct cctgtccacg
     4621 caggaacacc gcgagcagcg ccccgaacac cgcgaacggc acggccagga tcaccgcgaa
     4681 cggcagcgtc cacttctcgt actgcgcggc gagcacgagg aacaccatga gcacgccgac
     4741 caggaacacg acgttggagg tgccgcccga gaccttctcc tggaacgacg tgcccgtcca
     4801 ctcgagcgcg tacccgtccg gcagtgcggt gcgcgccacc tcctccatca ccgcgagcgc
     4861 ctcgcccgag ctgtatcccg gcgccgcctc gccgagcacc ttggccgatg gaaacacgtt
     4921 gtagcgctcg atgacctcgg ggccggtgga ttcgcggatg gtcacaagcg cggtgagcgg
     4981 gatcatctgg ccgtcgcccg cgcgcacgta aacgtcgcgg atgtcgtccg ggtgcgcgcg
     5041 gaactcggcc tcggactgca ggtgcacgcg gaacacgcgc ccggcgcggt tgaagtcgtt
     5101 gacgtagagc gcgccgaacg tgctctgcag cgtctcgaac acctcggcga cgtcgatgcc
     5161 gagcagcttc gcctgttcgc ggtcgacgtc gatccgcacc tgcggcacgc tcgccgagaa
     5221 cgtcgtcgtg acgttgacga gctccttgcg ctgcgacgcc gccgcgacga gcgactgcgc
     5281 cgccgcctcc agctccttca tcgagccgcc gccccgcgac tgcacgtagc cgtcgaaacc
     5341 gccggtcatg ctgaggccct cgatcggcgg cggctcgaac gcgagcacga aggcgtcctt
     5401 gatcgccgcc ccggccccga acacgtagcc cgcgatcgcg ctcgcggacg cgtcaggcgc
     5461 cgtgcgctcc gaccagtcct tgaagctgac ccaggtgatg ccggtgttcg tgcgcagcgt
     5521 gaacgtgagc gcgtcgagcc cggagaacgt catgacctgg tccacgtacg gattggcgag
     5581 cagcgcctcg gtgatctgct cgctgacggc ctcggtgcgc tgcagcgagg ccgcgtccgg
     5641 caggatcggg atcaccatca ggtagccctg gtcctccttg ggggccagcg cgttcggcac
     5701 ggcacgggcc agcgcgatcg tggcgacgag catcgcggcg accagcccga gcgcgagcgc
     5761 cgtgtgccgc agcaggtagc gcacgccgtt ggcgtaacgg cccgtcatcc gcgcgaacca
     5821 gtcgtggaag cgttcgagca gcccatggct caccgcgtct tcctggcgca gcagcgatgc
     5881 gcagagcgcc ggcgtcagcg tcagcgcgac gaagccggag atcgccaccg acaccgcgat
     5941 cgtcacggcg aactggcgat acatctcgcc gaccagcccg ccgaggaagg ccaccggcag
     6001 gaacaccgcg gtcagcacga acacgatcgc gatcaccggc cgcgtgacct gctgcatggc
     6061 cttgtgggtt gccgcgcgcg ggtcgaggcc ttcggtcgcg atgatgcgct cgacgttctc
     6121 gagcacgacg atcgcgtcgt cgaccacgat gccgatcgag agcaccatgc cgaagagcgt
     6181 cagcgtgttg atcgagaagc cgaacgccag catcgcggcc agcgtgccga tcaacgatac
     6241 cggcacggcc agcatcggga tcacggtcgc gcgccagttc tgcaggaaga cgaacacgac
     6301 caggaacacg agcgccatgg cctcgagcag cgtcacgagc acttcgcgga tcgagacctt
     6361 gatgtaggtc gtcgtgtcgt acgggtaact gtactcgagc ccggccggga agcgcgcctc
     6421 gagttcctcc atgcgcgcgt cgatcgcgtc gctgacctcg agcgcgttgg cgccgggctg
     6481 caggaagatg ccgatcggca cgctcgcctt gccgttgcgc gtcgccgaga agtcgtattc
     6541 gcgcgagccc agctcgacgc gcgcgacgtc ctcgaggcgc acgatgccgc cggactccga
     6601 ggtactgacg acgatccggc cgaagtcctc gggatcctgg agccggccct gcgtcgtgac
     6661 ggagaacgtg aagtcgaccc ggtcgtccat cggttcctgc ccgatgcgac ccgccgcgaa
     6721 ctgggcgttc tgctcgcgca ccgcgtcggc cacgtcgccc ggcgtcaggc cgagcttcgc
     6781 cagcacgtcg ggccgcagcc agatgcggat cgaccagtcc ttggagccga agatctgtgc
     6841 gtcgcccacg ccggggatgc gccgcagctc gtcgacgatg ttgagcagcg catagttcga
     6901 gatgaacagc gcgtcgtagc gcgggtcgtc cgaatcgagc gcaatgacct tcaggaacga
     6961 ctgctggctc ttgcgcaccg tcacgccctg ccgccgcact tcctcgggca gcgtcgacaa
     7021 cgcgctctgc acgcggttgt tgacgttgat cgcgttcagg tccggatcgg tgccgatcgc
     7081 gaacgtgact gccatcgaca gcgcgccgtt gccggcgctc gccgaccgga cgtacagcat
     7141 gccgtcgacg ccgttgacgg cctggtcgag gggcgcagcg accgtctgcg cgacggtctc
     7201 ggcgttggcg cccgggtaga acgcctgcac ctcgacctgt ggcggcagga tgtccgggta
     7261 ttgcgcgacc ggcagcacgc gcagcgcggc gagccccgcg atcacgatga atgccgagat
     7321 caccgacgcg aagatcggcc ggtcgatgaa atagctcgaa atcactgcgg cgccccgccc
     7381 gcagcgccgg tgggcggctc cggcgtggcc accttgacct tgacgcccgg acgcaccttg
     7441 agcactcctt cggcgacgac gcgctcgccg ccctgcagcc cttcgccgat caacgcgagg
     7501 ccggcggcgt cgccctgcag ccgcacgggc tgcagctgca cggtgtcgtc ggcgccgacg
     7561 acccagacga agtggccctg ggcgctcgac atgacggcct tgcgcgggac cgcgacggca
     7621 tcgggttcgg cgatgccctc gactcgcacg cgcacgaact gcccggggac gatgcgtcga
     7681 tcggcattgt cgaactcggc ccgcacgcgc accgtgccgg aacccggctc gaccgacgtg
     7741 tccacgaacg acagccgccc cgcgaccgcg tcggctcgtt cgccctcgag catcagcttg
     7801 acgacgagtt gctcgggcga ccggcggatg cgggccgctt ccgcctcggg caccgaaaac
     7861 tcgacgtaga tggggtgcac ctggacgatg cgcgtgagca ggctcgcttc gctgcccgcc
     7921 tggacgaggc tgccttcgga gcgcacctcg cggctggtga gaccgctgat cggcgcgcgg
     7981 acgtcggtgt accccaggtc gagctccgcc gtgcgcagcc gggcgcgcgc cgccgcgacg
     8041 ctggcctgcg cgacctcgaa ctgcgagacc gcctcgtccc gctggcgctg gctggtcgcg
     8101 ttctgctcga acagcggcac gatgcgatcg cgctgccggc gcgcctcttc gaagcgggcc
     8161 tgcgcgactc ccagttccgc gcgcgcctgc gcgaccgcgg cccggtacgg ttcgggatcg
     8221 atccggaaca gcacgtcccc ctcgcggacc tcgctgccct cgcggtagcg ccgctcgagc
     8281 aggatcccgt cgacccgcgc gcggacctcg acttcgcgcg atcccgcggc ccgcccggtg
     8341 aactcgagca ggatcggcac gctgccacgg gtgacctgca cgacgcccac ctcgggcggt
     8401 ggcatctcgc cgccctgctg cgactgcgac tggccgcaac cggcggcgag cgccaccagc
     8461 ggcaacagca aggccagcgg ccggcgcgaa ggcccgcctg cggtgtgtgt catggttgtt
     8521 gttctccgac tcggtcgcgc tcccgcccga agaccgtgcg gggaggtgcg gggagggccc
     8581 gaagagcgcg cactctaccg cccgtgccgc ccgccggaaa gcggaaaacg gcgaggcaca
     8641 tgacgttgcg ggcgcgaccc aagtaccatg cgcggccacg ctcaccttcg aggctccaat
     8701 gcgcacatcg acccgctccg cggtcttcgc cgcccttgcg tcctgcaccc tgcccgcgct
     8761 cgcggccgag cccgcgatgg acgaggctgc gcggatgcag tacgcgctcg gctaccagct
     8821 cggcaaggac ctgacggcgg tcgaatcgcg gccgcaggac ctcgcgcgcg gcgtcgagga
     8881 cggccgcagc ggcgcaaagc ccaagctgag cgaagccgag atggaggcag cgctcggttt
     8941 gctgcagcag cgcgtcaccg agcagcgcac caagtcgcag gccgagcagg ccgagaaggc
     9001 acgtgcctct ggcgaggcct acctcgcggc caacgcgaag aagcccggcg tcacgaccac
     9061 cccgagcggg ctgcagtacc gcgtcgtcac ccagggtacg ggcaagaagc cggtagcctc
     9121 cgagacggtc accgtgcact acaagggcac gctggtcgac ggcaccgagt tcgacagctc
     9181 gtacaagcgc ggccagcccg ccacgttccc cgtctccggc gtgattcccg gctggaccga
     9241 ggcgctgcag ctgatgccga tcggctccaa gtacgagctc gcgatccctc ccgcgctcgc
     9301 ctacggctcg cagggaccgc tcgccaacca ggtgctgctg ttcgaggtgg agttgctcgg
     9361 gaccaccgcc gacgcagacg acgccgcgaa gtaacgctcg ccggacgact catgtcgatc
     9421 gcccaggtcg acggcgtgca gatcgcctac gaagtgcgcg gcgaagcggc cgcgccggcc
     9481 gtgctgctgg tgatggggct cggcatgccg gcggccatgt ggcctgacga gttcgtgcag
     9541 gcgttgctcg atcgcggctt ccgcgtcgtc acgttcgaca atcgcgacag cggtgcgtcg
     9601 actcgattat ccgggctgcc ggtgcccaac atcccggtcg ccatgacacg cgcgctgatg
     9661 gggctgcgcg tggccgcgcc gtacacgctc accgacatgg cgatcgacgc ggccggcgtg
     9721 ctggacgcgg ccggcatcga ggccgcgcac gtcgtcggcg tgtcgatggg cggcatgatc
     9781 gcccaggtgc tggcggcact gctgccgcag cgggtcctga cgctcacgtc gatcatgtcc
     9841 tcgagcggca atccggcgcc gcgcgtggcc ctcggagacg cacgcgcgac gcgcgcgctg
     9901 ctgcggcgcc cgccggccga ccccgccgac ggcgaggcga tcgtcgaaca cctggtgtac
     9961 gtcttcggcg tcatcggcag cccgggttat ccgcaggacg acgccgcgcg ccgcgctcac
    10021 ctgcggcgcg tggcaggttg cggcctgtat cgcgaaggca cgatgcgcca gctcggcgcg
    10081 atcctggcgt cgggcgaccg gcgcacgatg ctgcgcaacg tcgaggcgcc gacgctcgtg
    10141 atccacggca ggagcgatcc tctcgtgccg gtcgccgccg gccgcgacac cgcgcggtac
    10201 gtgcgcggcg cgcagctcga gatcatcgac ggcatggggc acgacttccc gcccgcgctg
    10261 atgacccgca tcgccgagcg cgtcgcggcg cactgcgccg tatcagcctg acggagcgcg
    10321 acgcgatgcc ctgtagtgcg cggccgcctc cggatacagc acttcgagcg cgcgccagag
    10381 caggtcgtgg agcgcggcgg ccgcgtcgtc cgtcccgggc gccggattcc tcaatccggc
    10441 gaggagatcg gccgcgacac ccggtcgtgc cgacaccagc cgcgtcgcga cttcctgcca
    10501 gcgggggtcg gccgtgccgg cccggaactc gccccgaccg cggccgtagt gcgccaccgc
    10561 caggtagcgc acgatcgccg cttcgacgcg cgaggtgagg aactcgtccg accagcgcac
    10621 ggtgctgccc gcgaccccgc gctcggcgtt gtagagttgc gtgagcttgc gcgcgccgaa
    10681 cgcgccggcg aggccaccga gcatggcacc ggcaccgaag gtgagcccgc ccgcggcgag
    10741 gtccgcggca agtccgctca gtgcaccggt gacgatggcg ccgaacagtc cggcgcgatc
    10801 ggcgtcggcc gcgcgctcgg tctcgaacgg acgtgcgagg ttcgcgagga tctcctcgct
    10861 cgcgccgccc gtgagcccgt gcagcgcgac gagttccgcg gtgccttcgc gcacggcctc
    10921 gtcgagccgc gcgagcagcg cgcgctgggc cccttgctcg gccgggtcga cgtcaccgcc
    10981 gccgcgaaac aggccgcgcg ccttgtccgc gagcgacgcc tcgccgactg cctccgcgtc
    11041 gctcgcgacg cgtgccatct gccgggcgat cacggcggcg ctctgctcga ggacctgctc
    11101 gtcgcgtgcc gcccacgcct cgcgcaatcg cgcgaacgcc ggctgcactt cgtcggagag
    11161 gcactcgacc acggcctgca gcagcgtgtg ttcctgcagc cagcagcggg cgaatgcgtc
    11221 gaacggcagc acgcggcggc ctccgggcac cccggcagag tccagcagcc tctgccaccg
    11281 gctcgccacc tccagctcgc gacccgcgtc ggagtgcgtg ccgagctggt tcagcaacac
    11341 gagcgttggc ttgccgagcc actccaggat ctgcagctcc ggcgcgacgt agcccgcggc
    11401 gtccggatcc tcggtggcat tcgcgacgta cagcacgacg tcggcggaat cgcgcgcggc
    11461 ccgcatcgcc tgctggctgc accagaaggg acggtccgcg aaccggtccc agacctgcga
    11521 caggaaccag ccgagcggct ggccgctctg cgcgagcctg cgcgccagcc gcacgctgtc
    11581 gccgaagccc ggcgtgtccc acagcacgag ttcgtcgccc tcgtccgtgc gcaacagcac
    11641 gtgggactcg gccgtctcgg tcacgtgcgc gcggtccatg acggcaccga tgtcccggcg
    11701 cagcagcgtg cgggcgagcg tggtcttgcc gacgttggtg tgcgacagca gtgcgagctg
    11761 gatcgtgctc atgcgagcac gggtcgcgcc gacgcgcgca ggcgcccgac gagttcgggt
    11821 ggcacgtcct cgacccgccc gagcacaccc aggtccgcga tgcaggcctc ggcgccgtga
    11881 cgagcgacga actcgcgcca cgcgccacgt cgctcctcga cgcgcgcatc gaacgacgcg
    11941 tcgccgcgcg cgtgcgccac gaacggcgtt tcgtcgacga tcacgagcag tcgcgccgcg
    12001 gagcgcgccc gcgcgagggc gtcacgcagg gcgaccagca ccgcgccgtg attctcggct
    12061 tccggcgtgg cggccagcgt gaagagtatg gcttccacgt caccttgcgt gacgggtcgg
    12121 ctgcgcaggt cgtcttcgct gccgtaggcg atcggttcac ccagttccag tttcgtaccg
    12181 ggcccgtgcg tggcctgcaa cagcacgttg agtccagcga ggctcgaagt gtcgggtcgg
    12241 tacgcatagg gcgtcacccg tacggtggcg gcggccgggg cggcgtcgct cgcgccgagc
    12301 acgccgcggg cgtacggcag cagcgcgtcc ggcggcgcga gcgagcgcga cagtctcgcc
    12361 agcgagatcc acgaccacgc cgcgagcagc aggcgcggca acaccacgaa cagcagcgcc
    12421 gtggcggcgc tcaggtggat ccacggggcg gccggcccgc cgccgctgcc accccgccag
    12481 tgcaacgcgt cgatggtcgc atcgtccgcg ggcagcgcga tgcccgtcac cgcagacgcc
    12541 ggtccgtaga gcgcgtgcag cagcgtgcgc acctggcccg gcgtcaggaa cgtgctctcc
    12601 cagcccgcgc gatactccag ggcgatgccg cggacataga ggcccgcgac gaggccgatt
    12661 gcgagcgcag ccgcggcgac gtggaacact cgcttgccct gcagcgccat caggggctgc
    12721 gcgtgcggcc accagtcgtc cgcgaaacgt cgcagtgccg cggcgagcgg ccggtgatag
    12781 aaggaggcct ggcgcaccag cgcggccgcg cgtcgccagc cccaccgcag cggccaggcg
    12841 acccagccgg ccgtcgcgcc ggcagtcgcc cgcgcgccgt tgggccggcg gcgcaacgcg
    12901 gcgatcgcga gcaccaggta cacggcgagg ttccacagca cgacgccgag cagcgggaac
    12961 gcgaggacct cgatgcgcac gcgactgtcg accagcgaca gcacgacgcc gctggcgaac
    13021 gcgaccagca cgaccagcag cgacgcctgc gacgcatggg actcgagcat catcgtgcgc
    13081 ccgaccacgg ggtggcgctg caccagccgc tggtacagtt cgctcgcgcg gcggtccagc
    13141 acgcgccagg tgcgcgcgtc gcgaccggcc gaagtctcgc tggcggggtg cgcgcgcacc
    13201 gcttcgcgcg tggcggcttc gcgctcgcct tgcgtcagca ccgccccgtc ggcgtcatgg
    13261 tcctcgaccg acttgacgag caggatcctt ctcagagcat ccgcgtgcat gagcggccgt
    13321 cgctgaaccg tggtccgcgc gagtctagca agggctggcc ggtcgggtcg tccatgctaa
    13381 ggtgagcggc gcaggtgccc tccgtgttcc gcaagttcga gtccctggtc cagacctacc
    13441 ccgacgcgcg gccggcgacg ccgccgcgcg cgttcttcgc gttcctgtgg gactgcacgc
    13501 gcggcatgcg cccgtggatc ttcgccatga ccttctgcgg cgcgctgatc gccgcgttcg
    13561 aggcgctgct gtttgcgatg ctgggccgcg tcgtggactg gctcgccgag gtggagcctg
    13621 cgcagctctg ggcgcaggag cgcgccagcc tgctgctgct cgccgccgtg atcgcagcga
    13681 gcccgctcgt cgtgtgggcg ttctcgctga tcaagtacca ggtgctgttc gccaactttc
    13741 cgatgcggct gcggtggaac ttccaccggc tgatgctcgg ccagagcatg agcttctacc
    13801 aggacgagtt cgcgggacgc gtcgcgacca aggtcatgca gaccgcgctc gccgcgcgcg
    13861 acgcctggat gaccacgatg gacatcctcg cgttcatcac catctacttc atcacgatgc
    13921 tgtcgatcgt cgggacgttc gacctgtggc tgctggtccc cttcgtcggc tggctcgtgc
    13981 tctacgtctc gtgcctgcgg ttcttcgtgc cgcgactcgg ccgcgtctcc gcggcgcagg
    14041 ccgacgcacg cgcgctgatg accgggcgcg tcacggacgc ctacacgaac atcgccacgg
    14101 tcaagctgtt ctcgcacgcg agccgcgaag ccggctacgt aaaggcgtcg atgcaggagt
    14161 tcatgcagac cgcctacgcc cagatgcggc tggtgagtgg cttcgagatc gcgaaccacg
    14221 tattgagcat gctgctgatc gcgagcaccg ccggtgcggc gctgtggctg tggatcggcg
    14281 gcgaggtggg catcggcgcc gtcgcggccg cgacggccat ggcgctgcga ctgaacggca
    14341 tctcgcactg gatcatgtgg gagatggcct cactgttcga gcacgtcggc acggtgcagg
    14401 acggcatgcg cacgctctcg aagacgcaca ccgtcgtcga cgcgccgggc gcgccgccac
    14461 tcgtggtttc gcgcggcgag atccgcttcg agcacgtgac gttcggctac ggcgggcacc
    14521 gcgcggtcgt cgacgacctg acgctgcaca tccggcccgg cgagaagatc gggctggtcg
    14581 gtcgctccgg cgcgggcaag agcacggtcg tcaacctgct gctgcggttc tacgacgtcg
    14641 agcagggccg catcctgatc gacggccagg acatcgcgac cgtgcagcag gacagcctgc
    14701 gcgcgcagat cggcatggtg acgcaggaca cctcgctgct gcatcgctcg gtgcgcgaca
    14761 acatcgtcta tgggcggccg gacgcctccg agcaggacat ggtcctggcg gcccagcgcg
    14821 ccgaggcgca cgacttcgtg ctcgggctgg tcgatgcgaa aggacggcgc gcgtacgacg
    14881 cgcacgtggg cgagcgcggc gtgaaactct cgggcggaca gcgccagcgc atcgcgatcg
    14941 cgcgcgtgat gctcaaggat gcgccgatcc tgctgctcga cgaggcgacc agcgccctcg
    15001 acagcgagat cgaggcggcg atccagcaga gcctctaccg gctgatggag ggcaagacgg
    15061 tggtcgcgat cgcgcaccgg ctctcgacga tcgccgcgat ggaccgcctg atcgtcatgg
    15121 acaagggccg catcgtcgag gagggcgacc atcgctcatt gctcgcgcgc ggcgggctgt
    15181 acgcgcggct ctgggcgcac cagagcggcg gcttcctcgg cgaggagacc ggcgacgagg
    15241 acgacgtcgc caccgcggca cgcgccgcgg cgtcgggctg aacgccggcc cgcacgcccc
    15301 gctccgctgg cgtccacgcg tgtcgagacg cggattgaga cgcagtcgat ggcgcgggtg
    15361 acgcgcgccc gctgaaatcc ttgccattgc ggatatttcc gtactgcgct gcaggcgtgc
    15421 ggcgctagcc tatgtcggaa gccgcggcga gggacgctcg gcgacgacga gcacaggacg
    15481 actggagcgg cggcgatgag catcaacgaa ctgatcctgg tcggcacggg tttcgtgctt
    15541 gcgcgggtcg tctacctgtt ctacttctcg cagagaaaga ccgccgaagc ggaccgttcc
    15601 tgagcgccgt tttctacctc ccgcgctcga tctcgaccgt cacgtgcacc agttcatcgt
    15661 gcacgctgag ggcctgacgc actgactccg cccgcagccc gtcgtgctcg gcgccgaggc
    15721 tgacgatgca ggcgtacttg ccgcgcccga cccgccagac gtgcaggtcg agcagccgcg
    15781 gtgcaccggg cagcgacgca acgacctccc gcacttcgtc aaccacgggg gcgtccatct
    15841 cggcgtcgag cagcacacgg ccg
//
LOCUS       DASPFR010000253         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_682468, whole genome shotgun sequence.
ACCESSION   DASPFR010000253 DASPFR010000000
VERSION     DASPFR010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682468"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VD737_06720"
     CDS             <1..913
                     /locus_tag="VD737_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXW10289.1"
                     /translation="ISFGLMMLAPVLHEFASEEQRRRFLPPIARGEIRWCQGYSEPGA
                     GSDLAGLKTRAEDLGDHWLVNGQKIWTSYADQADWIFCLVRTNAESKHGGISFLLIDM
                     STPGIEVRPIQLISGKSPFCEVFLTDVRVPKENLVGKPGEGWKIAKRLLQHERASIST
                     HLQTGLGGSPTGRSLADLARASVGERDGRIADAHLRDAVARHEMKSEAIAITLKRVQE
                     ESGRHGPSAASSITKSLGAQHNKDKHELMVEILGLAGVGWEGEPFTTEELEVCRQWLR
                     SKGNSIEGGTSEIQTNIVAKQVLGLPG"
     gene            924..2066
                     /locus_tag="VD737_06725"
     CDS             924..2066
                     /locus_tag="VD737_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXW10290.1"
                     /translation="MGLVLDHDQALLRNTANDFASQRLPVTHLRRLRDTRDATGFDRE
                     AWTEMAGLGWTGMLIPEALGGNGFGYFGLGLVFEALGRTLAASPLYSTVGLSGTALGL
                     VGDDPRAHDLLRAIAAGESVVAFAHEEHARHRPWSIATTARRDGDGWLLDGRKRFVAD
                     GHVADALVLVARTSGGPDDLDGLSLFVVDGSAPGVTRRRLSLADSRNHADVELSNVRV
                     SGSALLGAEGGAGAVLARVLDVGRILLAAEMFGMAQQAFETTLDYLKQRQQFGVPIGS
                     FQALQHRAVQMYTEIELSRSVLYEALTALDDGRWDDVPRLASVCKALLNDTLHLVSNE
                     AVQMHGGIGMTDQVDVGLYLKRARVAEAMLGTSAFHRERYADLQRF"
BASE COUNT          328 a          720 c          751 g          311 t
ORIGIN      
        1 gatctcgttc gggctgatga tgcttgcgcc ggtgctgcac gagttcgcca gcgaggagca
       61 gcgtcggcgc ttcctgccgc cgatcgcgcg cggcgagatc cgctggtgcc agggctactc
      121 ggagccgggc gccggttcgg acctcgccgg gctcaagacc cgggccgagg acctcggcga
      181 ccactggctc gtcaacggcc agaaaatctg gacctcgtac gcggaccagg ccgactggat
      241 cttctgcctg gtgcgcacca acgcggagtc gaagcacggc ggcatctcgt tcctgctgat
      301 cgacatgtcg acgcccggca tcgaggtacg gccgatccag ctgatctcgg gcaagtcgcc
      361 gttctgcgag gtgttcctga ccgacgttcg cgtgcccaag gagaacctcg tcggcaaacc
      421 gggcgaaggc tggaagatcg ccaagcggct gctgcaacac gagcgcgcca gcatctcgac
      481 gcacctgcag accgggctcg gcggctcgcc gaccggccgc tcactcgcgg acctcgcccg
      541 ggcgagcgtc ggggagcgcg acggacgcat cgccgatgcg cacctgcgcg atgcggtcgc
      601 gcgccacgag atgaagagcg aggccatcgc gatcacgctc aagcgcgtgc aggaggagtc
      661 cggccgccac ggaccctcgg ccgcctcgtc gatcaccaag tcgctcggcg cgcagcacaa
      721 caaggacaag cacgagctga tggtcgagat cctcggcctc gcgggcgtgg gctgggaggg
      781 cgagccgttc acgacggaag agctcgaggt gtgccggcag tggctgcggt cgaaaggcaa
      841 ctcgatcgag ggtggcacca gcgagatcca gaccaacatc gtggccaagc aagtgctcgg
      901 cctgccgggc tgaggggaag atcatgggac tcgtgctcga ccacgaccag gcgctgctgc
      961 gcaataccgc caacgacttc gcgagccagc ggctgccggt gacccacctg cgtcgcctgc
     1021 gcgacacgcg cgatgcgacc ggcttcgacc gggaggcgtg gaccgaaatg gccggcctcg
     1081 gctggaccgg catgctgatt cccgaggcgc tgggcggcaa cggcttcggg tatttcgggc
     1141 tcggcctggt gttcgaggcg ctcggtcgca cgctcgccgc ctcgccgctc tattcgaccg
     1201 tcgggctgtc cgggaccgcg ctgggcctgg tcggcgacga cccacgtgcg cacgacctgc
     1261 tgcgcgcgat cgcggccggc gagtcggtcg tcgcgttcgc gcacgaggag cacgcgcggc
     1321 accggccctg gtcgatcgcg acgaccgcgc gtcgggacgg cgacggctgg ctgctcgacg
     1381 gtcgcaagcg cttcgttgcc gacggccacg tcgccgatgc gctggtcttg gtcgcgcgga
     1441 caagcggcgg gcccgacgac ctcgacggac tgtcgctgtt cgtggtcgac ggctcggcgc
     1501 cgggggtcac gcgtcgccgg ctgagcctcg ccgacagccg caatcacgcc gacgtcgagt
     1561 tgtcgaacgt gcgggtgtcg gggtccgcac tgctcggcgc cgagggcggc gccggtgccg
     1621 tgctcgcgcg cgtgctcgac gtgggccgca tcctgcttgc cgccgagatg ttcggcatgg
     1681 cgcagcaggc gttcgaaacg acgctcgact acctcaagca gcgccagcag ttcggcgtgc
     1741 cgatcggcag cttccaggcg ctgcagcacc gcgccgtgca gatgtacacc gagatcgagc
     1801 tgtcacgctc cgtgctctac gaggcgctga ccgcgctcga cgacgggcgc tgggacgacg
     1861 tgccgcggct cgcgtccgtg tgcaaggcct tgttgaacga cacgctgcac ctggtcagca
     1921 acgaagccgt gcagatgcac ggtggcatcg gcatgaccga ccaggtcgac gtgggcctgt
     1981 acctgaagcg cgcccgcgtg gccgaggcga tgctcggcac gtcggcgttc caccgcgagc
     2041 gctacgccga cctccagcgc ttctaaatgg ggacaccccg gaaaatgggg acattccgaa
     2101 tttccgcctg
//
LOCUS       DASPFR010000254         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1414771, whole genome shotgun sequence.
ACCESSION   DASPFR010000254 DASPFR010000000
VERSION     DASPFR010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414771"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..730)
                     /locus_tag="VD737_06730"
     CDS             complement(14..730)
                     /locus_tag="VD737_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXW10291.1"
                     /translation="MSRKSRRNRPVVPASPATETAVVVHRNRRRGIVAIGLALLVVVA
                     LAVYALRDGPVPGEGDARTAALASEHSPTLGDAAAKVHVVEFLDPACETCAQFYPVVK
                     QLIEQNPGRIRLSVRHVAFHEGADYVVRLLEASRAQDRYWETLERLLQTQAQWAPHHT
                     VRPDLVEPIVASLGLDMQRLAADMESPEVAQRVARDRDDAIALKVTATPEYRVNGRPL
                     PEFGYEPLLALVREELERSY"
     gene            complement(847..1548)
                     /locus_tag="VD737_06735"
     CDS             complement(847..1548)
                     /locus_tag="VD737_06735"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXW10292.1"
                     /translation="MTIEYDGLAPPSRRETLQELRLPLDALRWAPKWFGLPRGRAATS
                     RTVVLLPGFGAGPRSLVALKRHLRRRGHAAWDWGLGRNTGRVPQLRDALRERVETLAR
                     ETGGPVTLVGWSLGGYIARELAREHPELVRRIVTLGSPVVGGPRFTATAHWYVSQGFD
                     LDQIERAVKDRYAKPLRTPVVAIYSKRDGVVAWQACIDRWSPDVRNVEVDESHFGMGF
                     APDVLRIVAEEVERD"
     gene            1665..>2633
                     /locus_tag="VD737_06740"
     CDS             1665..>2633
                     /locus_tag="VD737_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017692970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HXW10293.1"
                     /translation="MPTRRRPTNRLLTALRPDSFTLMMLATVALASLAPARGAAVPTL
                     DALVKASVMALFFLHGARLAREAVLQAVGHWRLQFMILALTYLLFPAIALGVGPLASR
                     TLEPALYLGFVFLCCLPSTVQTSIAYTSVGRGNVPAAACAASASNLLGIVFTPLLTGL
                     LLARQGTVTLGALGTIVVLLLLPFVAGQVARPWLGGWVARRRLLLAIVDRGAILLMIY
                     SAFGKAVDAGIWQRVSAGDLVLVVLVSIVLLAAVVATAIVAARRAGFSREDEVAIVFC
                     GSMKSLVTGMPMANVLFPGAQAGLLVIPMIVFHQVQLLSCGVLARRY"
BASE COUNT          329 a          949 c          942 g          413 t
ORIGIN      
        1 gcggcggcca tggtcagtag ctccgctcga gttcctcgcg caccagcgcg agcagcggct
       61 cgtagccgaa ctcgggcagc ggacgtccgt tcacgcggta ctcgggcgtc gccgtgacct
      121 tgagcgcgat ggcgtcgtcc cggtcgcggg cgacgcgctg cgccacctcg ggcgactcca
      181 tgtctgcggc gaggcgttgc atgtccagtc cgaggctcgc cacgatcggc tcgacgaggt
      241 cgggccgcac cgtgtgatgc ggcgcccact gcgcctgcgt ctgcagcagg cgctcgagcg
      301 tctcccagta gcggtcctgc gcgcgactcg cctcgagcag ccgcacgacg tagtcggcgc
      361 cctcgtggaa cgcgacgtgg cgcaccgaga gccggatgcg acccgggttc tgctcgatca
      421 gttgcttgac gacggggtag aactgcgcgc aggtctcgca ggcggggtcg aggaactcga
      481 ccacgtggac tttcgcggcc gcgtcgccga gcgtgggcga gtgctcgctg gcaagggccg
      541 cggtgcgcgc gtcgccctcg ccgggcaccg gtccgtcgcg cagcgcgtac acggcgagcg
      601 cgacgacgac gagcagcgcg aggccgatcg cgacgatgcc gcggcgacgg ttgcggtgca
      661 cgacgaccgc ggtttcggtg gccggcgagg ccggcacgac gggacggtta cgcctggact
      721 tacggctcat ctctgcgctt gctccacgcg gcacgggtgc gccgccgcag cgttcgacgc
      781 cgcagcgtcc cggaggttcc ggcgcggacg ggctctacct gcagctcccc cggcatccac
      841 gtatgctcag tcgcgctcga cttcctcggc gacgatgcgc agcacgtccg gcgcgaagcc
      901 catgccgaaa tgcgactcgt ccacctcgac gttgcggacg tcgggggacc agcggtcgat
      961 gcacgcctgc caggccacca cgccatcgcg cttcgagtag atcgcaacga ccggcgtgcg
     1021 cagcggcttc gcgtagcgat ccttcacggc ccgctcgatc tggtcgaggt cgaagccctg
     1081 cgacacgtac cagtgcgcgg tggcagtgaa ccgcggcccg ccgaccaccg ggctgcccag
     1141 cgtgacgatg cgccgcacca gctcggggtg ctcgcgcgcg agctcacggg cgatgtatcc
     1201 gcccaggctc cagccgacga gcgtcaccgg tccaccggtc tcccgcgcga gtgtctcgac
     1261 gcgctcacgc agggcgtccc gcaactgcgg cacccgcccg gtgttgcgac cgaggcccca
     1321 gtcccacgcc gcgtgcccgc ggcgccgcag gtggcgcttg agggccacga gcgagcgcgg
     1381 acccgcgcca aagcccggca gcaggaccac ggtgcgggag gtcgccgcgc gcccgcgcgg
     1441 cagcccgaac cacttcggcg cccagcgcag cgcatcgaga ggcagtcgca gttcctgcag
     1501 cgtctcgcgc cgggagggcg gggcgaggcc gtcgtattcg atggtcatgt gaggtgggcc
     1561 cgggaggagc cacgattgtg cttgaagcgg cgcccgcgct ccactagcgt cccgctgcac
     1621 actggcgtcc gggacgccag cacgatcagc cctggagccg ttgcatgccg acccgccgga
     1681 gaccgacgaa ccggctcctc accgccctgc gccccgactc gttcacgctg atgatgctcg
     1741 ccaccgtcgc gctcgcgagc ctcgcgccgg cgcgcggcgc ggccgtgccg acgctcgacg
     1801 cactggtcaa ggcttccgtg atggcgctct ttttcctgca cggtgcccgc ctggcccgcg
     1861 aggcggtgct gcaggcggtc ggccactggc gcctgcagtt catgatcctg gcgctgacct
     1921 acctgctgtt tccggcgatc gccctgggcg tgggaccgct tgcgtcgcgc acgctggagc
     1981 cggcgctgta cctgggattc gtgttcctgt gctgcctgcc gtcgaccgtg cagacctcga
     2041 tcgcgtacac ctcggtcggg cgcggcaacg tgccggcggc ggcctgcgcg gcgtccgctt
     2101 cgaacctgct cggaatcgtg ttcacgccgc tgctcacggg cctgctgctg gcacgacagg
     2161 gcacggtgac cctcggtgcg ctcggcacga tcgtcgtgtt gctgctgctg ccgttcgtcg
     2221 cgggacaggt cgcgcggccg tggctcggcg gctgggtcgc ccgacgccgc ctgctgctcg
     2281 cgatcgtcga ccgcggcgcg atcctgctga tgatctactc ggcgttcggc aaggcggtcg
     2341 atgccggcat ctggcaacgc gtgtcggccg gcgacctcgt actcgtggtg ctcgtgagca
     2401 tcgtgctgct ggccgccgtc gtcgcgaccg cgatcgtggc cgcgcggcgg gcggggttct
     2461 cgcgcgagga cgaagtggcg atcgtcttct gcggctcgat gaaatcgctg gtcacgggca
     2521 tgccgatggc caacgtgctg tttccgggcg cgcaggcggg cctgctggtg atcccgatga
     2581 tcgtcttcca ccaggtccag ctgctgtcct gcggcgtgct cgcgcgtcgc tac
//
LOCUS       DASPFR010000255         7587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_74017, whole genome shotgun sequence.
ACCESSION   DASPFR010000255 DASPFR010000000
VERSION     DASPFR010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7587
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74017"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1696)
                     /locus_tag="VD737_06745"
     CDS             complement(131..1696)
                     /locus_tag="VD737_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HXW10294.1"
                     /translation="MGWYDVPRPIVPDLIVQHGRNQARKPALLEGTRRLDWGQFDAAT
                     NRVANGLQQLGLGPGHRLAVLMSNSIEMALVLFGAGKAGVSVVPLNVAVSDAAVAAMV
                     RDSGARAVAASGEHCGRIDALIAAGALEARITRIGVEVPDGGGWLEFDAWQAAAPATP
                     PDAVVTPQTECNIIYSSGTTGLPKGIVHTHGCRVHWATDLAIALRYHSGAVTVCSLGL
                     YSNISWVAMLCTVLVGGTIVVMPSFSAGSLAETIARFRITHGAFVPVQLQRLMDLEDL
                     ARRDFTSLQAIMCCGSPLQPVLKRRARDRLACELIELYGLTEGIVTTLAPEDFDRKLE
                     SVGKPIPGQQLVLVRDDDREVGPGEFGEICGYGRLVMEGYHNRPEATAEATWIDPQGR
                     KWLRTGDVGRLDDEGFLYVVDRKKDMILSGSQNVYPVDIETVMLGHAAVREVAVVGVP
                     SERWGETPLAVVVPAGTVDAADLVEWTNARVGRQQRIAGAVLVGELPRNPNGKILKRE
                     LRRVYAGWGDGAR"
     gene            complement(1714..3966)
                     /locus_tag="VD737_06750"
     CDS             complement(1714..3966)
                     /locus_tag="VD737_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXW10295.1"
                     /translation="MNKFAVHSARAAAFAASLFAVPPVLAQDAPVSIGAMGLEEVTVT
                     ARRREESLRDVPIAVSAFSAERIEATGAPDITWLQQTTPNLTLQVARGTNSTLVSFIR
                     GIGQQDPLWGFEPGVGLYVDDVYIARPQGAVLDIYDIERLEVLRGPQGTLYGRNTIGG
                     AIKYVTRPLGDQPRLDVKYTLGSYAQHDVLASGVLPLGDTFSVGASAAIYRRDGYGDN
                     KFTAEDHYAKDVEAYRLSADWRPSEDVSVRIAADTVIDDSPAKHGHREVPGAGLTTGE
                     VVLDDIYDTRGGAGSDNRVMTEGVAMTVAWNIDETLTFKSITAYRKGETDTLIDFDSS
                     PQPALDIPGAYADDQTSQEFQLLFEGDRWQGVAGLYYLDASASGAFDTPVGLLGATTF
                     TDGYVDTTSYAAFADVSYSFTDRLRASLGGRYTRDEKEGSVYRADFLGLGSPYFTNVD
                     AAPLRVRTDYTNERTFDKFTPRASVSFDFSDSLTTYASYSEGFKSGGFDMRGDAIFTP
                     DTVNGYDPENVKSFELGLKGSAWDGRATYNLAAFYSDYTDQQITRQEPTATGVASFVD
                     NAGSSTIQGVELEGAVAFTDQFSLTYGIGWTDAEFDEYASFTVVSGTPVPIDYADIAH
                     FQNTPEWNGNVALTYLQPLAPNWGSLATVVSASYRDSYYMFEFDRPLIDQTTDYTLID
                     ASITWTSASDRFRVQLSGRNLTDEEYKVGGYDFYTDYSAATQALFGNTVTSFYGPPRT
                     YSVSLSYRFD"
     gene            4119..4877
                     /locus_tag="VD737_06755"
     CDS             4119..4877
                     /locus_tag="VD737_06755"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HXW10296.1"
                     /translation="MQKKKTTTRTVTRARASRVAPGTGSRSAATRERILDAAERLFAE
                     RGYHGVSIRDVTGAAEVDVALVAYHFGNKQGLLEAVFLRRAADLNDERIALLDGVLAR
                     SGRRAPRLEDIIDAFTRPLLDRSTRGSAGWKSYFALIAEINNSPEFGGVLMTRFFDPV
                     VQRFIEAIRRALPHCDDRDLYWAYHFLSGALTLTFAETGRIDKLSGGVCRSSDLASVH
                     ERLVPYCAAGFRALCGNGRARAATPRVVSRRGRD"
     gene            4901..5650
                     /locus_tag="VD737_06760"
     CDS             4901..5650
                     /locus_tag="VD737_06760"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: protein motif:HMM:TIGR01963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HXW10297.1"
                     /translation="MVTGAGSGIGLGLAHHLAARGHEVWLSDLARAEVEQAADDLVRQ
                     GFAAHAVELDVTADRSIRAAVESLGDAGVDLLVNNAGLQHVDALDDFPPERWAQLVAV
                     MLTGPAMVTRALLPGMRRRNFGRIVNVGSIHSLVASPYKSAYVAAKHGLTGLARTLAL
                     ETADVDVTVNTICPAYVRTPLVDAQITAQARAHGIPEQRVIDEIMLKPMPKRAFIGID
                     ELGALIDYLASDAARNVTGQSIAVDGGWTAQ"
     gene            complement(5679..6986)
                     /locus_tag="VD737_06765"
     CDS             complement(5679..6986)
                     /locus_tag="VD737_06765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXW10298.1"
                     /translation="MPRGTDATTRVPGDQVTPATESARYRALVLVMLVLVYTFNFIDR
                     QIVGILAIPIKADLGLTDTQLGLMGGLAFALFYTLLGIPIAMLADRANRTVIMTVALV
                     VWSAMTAVCGFAQNFAQLFLARLGVGVGEAGGVAPAYSLVADYFPSEQRARALAVYSF
                     GIPLGSSLGILFGGVIASLIDWRTAFIAVGVAGVVLAPIFKWLVREPQRGRYDPRGAG
                     AQPASLREVLRVLVAKRSFWGLSLGASASSMMGYGLFFWLPSFFVRSHGLTLLQASLY
                     FAAILLVGGMAGIWLGGWSADRFGAGKRRAYALIPATAFMLTLPFYVVAVLAPSPALS
                     FALFLVPTALGLVWLGPVLSAIQHVVPSGMRATASAVFLFINNLIGIGLGTVVLGALS
                     DALSARYGENSLRYAILAGTVFYLVAAALFVLSARRLERDWEQ"
     gene            complement(6970..>7587)
                     /locus_tag="VD737_06770"
     CDS             complement(6970..>7587)
                     /locus_tag="VD737_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10299.1"
                     /translation="RGVRSANLVRIEREGAGHNLPIAAPGAAPCAAGEPPYVASCDYD
                     AARALLEHLYAPIAAPAPTAAAPAPLRTFDQRGYAAAAGSVSFADEGWLYVPTTCTEG
                     TSTPCRLHVVFHGCKQGAGEVGDAFVRRSGYLEAAEAGRIVVLFPQVKPTMQPLNPLG
                     CWDWWGYEGAEYATRRGPQIRAVRAMIDDLLGTPAREGAADAEGN"
BASE COUNT         1211 a         2642 c         2636 g         1098 t
ORIGIN      
        1 aagtcacgcg aattgcgcgt gaggcccggc aggcacagca ccggcaggcg cccggccgag
       61 cccgcgggat agtcgcggta gtacagccgc agcccgtcgg ccgagtcgaa ccagcgctcg
      121 gtcatgtcgc tcatcgcgcc ccgtctcccc atccggcata caccctgcgc agctcgcgct
      181 tgaggatctt accgttcggg ttgcgtggca actcgccgac cagcaccgcg ccggcgatgc
      241 gctgctgcct gccgacgcgc gcgttggtcc actcgacgag gtccgcggcg tcgaccgtgc
      301 ctgcgggcac gacgaccgcg agcggcgtct cgccccagcg ttcgctcggg acgccgacca
      361 cggccacctc gcgcacggcc gcgtgcccga gcatcacggt ctcgatgtcg accggataca
      421 cgttctggct gccggacagg atcatgtcct tcttgcggtc cacgacgtac aggaaacctt
      481 cgtcgtcgag ccggccgacg tcgccggtgc gcagccactt gcggccctgc gggtcgatcc
      541 aggtcgcctc ggccgtcgcc tccggccggt tgtgatagcc ctccatcacg agccggccgt
      601 aaccgcagat ctcgccgaac tcgccgggcc cgacttcgcg gtcgtcgtcg cgcacgagca
      661 cgagctgctg gccgggaatc ggcttgccga cggactcgag cttgcggtcg aaatcctcgg
      721 gggcgagcgt ggtcacgatg ccctcggtga gcccgtacag ctcgatcagc tcgcaggcga
      781 gcctgtcgcg ggcgcggcgc ttgagcaccg gctgcagcgg cgaaccgcag cacatgatcg
      841 cctgcagcga agtgaagtca cgtcgcgcga ggtcctcgag gtccatcagg cgctgcagct
      901 gcacgggcac gaatgcgccg tgcgtgatcc tgaagcgtgc gatcgtctcc gccagcgacc
      961 cggccgagaa cgacggcatc acgacgatgg tcccgccgac gagcaccgtg cacagcatcg
     1021 cgacccagct gatgttcgaa taaaggccca gcgagcagac ggtgacggcg ccgctgtgat
     1081 aacggagcgc gatcgcgagg tcggtcgccc agtgcacgcg gcagccgtgc gtgtgcacga
     1141 tgcctttcgg caacccggtg gtgccggaac tgtagatgat gttgcactcg gtctgcggcg
     1201 tcacgaccgc gtccggtggc gtcgcgggtg ccgcggcctg ccacgcgtcg aactcgagcc
     1261 agccaccgcc gtcgggcacc tcgacaccga tgcgcgtgat gcgcgcctcg agcgctccag
     1321 ccgcgatcag ggcgtcgatg cgcccgcagt gctcgcccga agctgccacc gcgcgggcgc
     1381 cgctgtcgcg gaccatggcg gcgactgcgg cgtcggagac cgcgacattg agcggcacca
     1441 ccgagacgcc ggccttgccc gcgccgaaca gcacgagcgc catctcgatg ctgttcgaca
     1501 tcaggacggc gagtcgatga cccggcccga gcccgagttg ctgcagaccg ttcgcgaccc
     1561 gattcgtagc cgcgtcgaac tggccccagt cgagccggcg cgtgccctcg agcagcgccg
     1621 gcttgcgggc ctggttgcgg ccgtgctgga cgatcaggtc cgggacgatc ggccgtggta
     1681 cgtcgtacca gcccatggcc ggatcccgcc gcgtcaatcg aaacgatagc tcaggctcac
     1741 ggaataggtc cgcgggggcc cgtagaagct ggtgaccgta ttgccgaaca gcgcctgggt
     1801 ggccgcgctg taatccgtgt agaagtcgta gccgccgacc ttgtactcct catccgtcag
     1861 gttgcggccg gacagctgca cgcggaaccg gtcgctggcc gacgtccacg tgatgctcgc
     1921 gtcgatcagc gtgtagtcgg tggtctggtc gatcaacggc cggtcgaact cgaacatgta
     1981 gtacgagtcg cgatacgagg ccgacaccac cgtggcgagg gaaccccagt tcggggccag
     2041 cggctgcagg tacgtgagcg cgacgttgcc gttccactcc ggcgtattct ggaagtgcgc
     2101 gatgtcggcg tagtcgatcg gcaccggcgt gccgctcacg accgtgaagc tcgcgtattc
     2161 gtcgaactcc gcatccgtcc agccgatgcc gtaggtgagc gagaactggt cggtgaaggc
     2221 gacggcgccc tcgagctcga cgccctggat ggtggagctg cccgcgttgt cgacgaagct
     2281 tgcgacgccc gtggccgtcg gctcctggcg cgtgatctgc tggtccgtgt agtcggagta
     2341 gaacgccgcg aggttgtagg tggcgcgacc gtcccatgcc gagcccttca ggccgagctc
     2401 gaacgacttg acgttttcag ggtcgtagcc gttgacggtg tctggcgtga agatcgcgtc
     2461 accgcgcatg tcgaagccgc ccgacttgaa gccctccgag tacgacgcat acgtcgtgag
     2521 cgaatcgctg aagtcgaagc tgacgctcgc gcggggcgtg aacttgtcga acgtccgctc
     2581 gttcgtgtag tcggtgcgca cccgcagcgg ggcggcgtcc acgttggtga agtacggcga
     2641 gccgaggccc aggaagtccg cccgatacac cgagccttcc ttctcgtccc gggtgtaacg
     2701 gccgccgagc gaggcgcgca gccggtccgt gaacgagtag ctgacgtccg cgaaggcggc
     2761 gtagctggtc gtatcgacgt atccgtcggt gaacgtcgtc gcgccgagca ggccgaccgg
     2821 ggtatcgaac gcgcccgagg ccgaggcgtc caggtagtac agcccggcca cgccctgcca
     2881 ccggtcgccc tcgaacagca gctggaattc ctggctcgtc tggtcgtcgg cataagcgcc
     2941 ggggatgtcg agcgcgggct gcggcgacga atcgaagtcg atcaacgtgt cggtttcgcc
     3001 cttgcgatag gcggtgatcg acttgaacgt cagcgtctcg tcgatgttcc aggcgaccgt
     3061 catcgcgacg ccctcggtca tgacgcggtt gtcggagccg gcgcctccgc gcgtgtcgta
     3121 gatgtcgtcg agcacgacct cgccggtggt gagcccggcg cccggaacct cgcgatgccc
     3181 gtgcttcgcg ggcgaatcgt cgatcacggt gtccgcggcg atgcgcacgc tgacgtcctc
     3241 gctcggccgc cagtcggcgc tcagccggta agcctcgaca tccttggcgt agtggtcttc
     3301 ggccgtgaat ttgttgtcac cgtagccgtc gcgccggtag atcgccgcgc tcgcgcccac
     3361 cgagaacgtg tcaccgagcg gcagcacccc ggaggccagc acgtcgtgct gggcatacga
     3421 accgagcgtg tacttgacgt cgagcctcgg ctggtcaccg agcgggcgtg tcacgtactt
     3481 gatcgcgccg ccgatcgtgt tgcggccgta aagcgtgccc tgcgggcccc gcagcacctc
     3541 gagccgctcg atgtcgtaga tgtcgagcac cgcgccctgt ggccgcgcga tgtagacgtc
     3601 gtcgacgtac aggccgacgc cgggctcgaa cccccagagc gggtcctgct ggccaatgcc
     3661 gcggatgaag gaaaccagcg tggaattggt gccgcgcgca acctgcagcg tcaggttggg
     3721 cgtcgtctgc tgcagccagg tgatgtcggg cgcaccggtg gcctcgatgc gttccgccga
     3781 gaacgcggac accgcaatcg gcacgtcccg cagcgactcc tcgcgacgtc gcgcggtgac
     3841 ggtgacttcc tcgaggccca tcgcaccgat ggagaccggc gcgtcctgcg cgagcaccgg
     3901 cggcacggcg aacagcgagg cggcgaacgc ggcggcgcgg gccgagtgga cggcgaactt
     3961 gttcatggat ccccctgcag tggcgccgga aggcgcaaac attcaacgcg cgttggaatt
     4021 tagttgaacg atcgttgaat ttcaacaagc gttgcaggtt gcgaccgggt tgcgcagaat
     4081 cgcggctcgg ccgcgcgccg ggggcagggc gtaacgcagt gcaaaaaaag aagaccacca
     4141 ccaggacagt cacgcgcgca cgcgcgtcgc gcgtcgcgcc cggcaccggc tcgcggtctg
     4201 cggcgacacg cgagcgcatc ctcgatgccg cggagaggct gttcgcggaa cgcggttacc
     4261 acggcgtgtc gatccgcgac gtgaccgggg ccgccgaggt cgacgtcgcg ctcgtcgcat
     4321 accacttcgg caacaagcag ggcctgctcg aggcggtgtt cctgcgccgt gccgcggacc
     4381 tcaacgacga gcgcatcgcg ctgctggacg gtgtcctggc gcgcagtggc cgacgggccc
     4441 cgcggctcga ggacatcatc gacgccttca cgcgcccgct gctcgatcgc tccacgcgcg
     4501 gcagcgccgg ctggaagagc tatttcgcgc tgatcgccga gatcaacaac tcgccggagt
     4561 tcggcggcgt gctgatgacg cggttcttcg accccgtggt gcagcgcttc atcgaggcga
     4621 tccgccgcgc gctgcctcat tgcgacgacc gggacctcta ctgggcgtac cacttcctct
     4681 cgggcgcgct gacgctcacg ttcgcggaga ccggccgcat cgacaagctt tcaggcggcg
     4741 tctgccgttc gagcgacctg gcgagcgtcc acgagcggct cgtgccgtac tgcgccgccg
     4801 gatttcgcgc gctctgcggc aacggtcgcg cccgggccgc gacgccgcgg gtggtctcga
     4861 ggcgggggcg ggattgagca gaccgcgtcg gctccgcgcc ttggtcacag gcgccggcag
     4921 cggcatcgga ctcgggctcg cccaccacct ggcggcccgg ggacacgagg tctggctgag
     4981 cgacctggcg agggccgagg tcgagcaggc ggcggacgac ctggtgcggc agggcttcgc
     5041 cgcccacgcg gtcgaactcg acgtcacggc cgaccgcagc attcgcgccg ccgtcgagtc
     5101 gctgggcgat gcgggcgtgg acctgctcgt caacaacgcg gggctgcagc acgtcgacgc
     5161 gctggatgac tttcctcccg agcgctgggc gcagctggtc gcggtgatgc tgaccgggcc
     5221 ggcgatggtc acccgcgcgc tgctgcccgg catgcggcgc cgcaatttcg ggcgcatcgt
     5281 caacgtgggc tcgatccact cgctcgtggc gtcaccgtac aagagcgcct acgtcgctgc
     5341 gaagcacggc ctgacggggc tcgcgaggac gctcgcgctc gagacggccg acgtcgacgt
     5401 cacggtcaac acgatctgcc ccgcctacgt gcgtacgccg ctcgtcgacg cgcagatcac
     5461 ggcgcaggcc cgggcgcacg gcatccccga gcagcgcgtg atcgacgaga tcatgctgaa
     5521 gcccatgccc aagcgcgcgt tcatcggcat cgacgagctc ggcgcgctga tcgactacct
     5581 cgcgagcgac gccgcgcgca acgtcaccgg gcagtcgatc gcggtggacg ggggctggac
     5641 ggcgcagtag gcccggtcac gcccgacggc gcaggcgatc actgctccca gtcgcgctcc
     5701 agtcgtcgcg ccgacagcac gaacagcgcc gccgcgacga ggtagaacac cgtgcccgcg
     5761 aggatcgcgt aacgcagcga gttctccccg tagcgtgccg agagcgcgtc cgagagcgcg
     5821 ccgagcacga ccgtgcccag accgatgccg atcaggttgt tgatgaacag gaacaccgcc
     5881 gaggccgtcg cgcgcatgcc cgacggcacg acgtgctgga tcgccgagag caccggcccc
     5941 agccacacca ggccgagcgc cgtcggcacg aggaacagcg cgaacgacag cgctggcgaa
     6001 ggggcgagga ccgcgacgac gtagaacggc agcgtcagca tgaacgcggt cgccgggatc
     6061 agcgcatacg cgcggcgctt gccggcgccg aaccggtccg ccgaccagcc gccgagccag
     6121 atgcccgcca tgccgccgac gagcaggatc gccgcgaaat agagcgatgc ctgcagcaac
     6181 gtgaggccgt ggctgcgcac gaagaacgac ggcagccaga agaacaaccc gtagcccatc
     6241 atcgaggagg ccgaggcgcc gagcgagagg ccccagaaag agcgcttcgc gaccagcacc
     6301 cgcagaactt cgcgcaggct ggccggctgc gcgcccgcgc cgcgcgggtc gtagcggccg
     6361 cgctgcggct cgcgcaccag ccacttgaag atcggtgcca gcacgacgcc ggcgacccct
     6421 accgcgatga acgcggtacg ccagtcgatc agcgacgcga tcacgccgcc gaacaggatg
     6481 ccgagcgagc tgccgagcgg aatgccgaac gaatacaccg cgagggcacg agcccgttgc
     6541 tccgatggaa agtaatcggc gaccagcgag tacgccggcg ccaccccgcc ggcttcgccg
     6601 acgccgacgc cgagccgcgc gaggaacagc tgcgcgaagt tctgcgcgaa cccgcagacc
     6661 gccgtcattg cgctccagac gacgagcgcg accgtcatga tcacggtgcg gttcgcgcgg
     6721 tccgccagca tcgcgatcgg gatgccgagc agcgtgtaga agagcgcgaa cgcgagcccg
     6781 cccatcaggc cgagctgcgt atcggtgagc cccaggtcgg ccttgatcgg gatcgccagg
     6841 atgccgacga tctgccggtc gatgaagttg aacgtgtaga ccagcaccag catcaccagc
     6901 acgagcgcgc ggtaccgcgc ggactcggtc gcgggcgtga cctggtcccc gggcacccgt
     6961 gtcgtcgcgt cagttcccct cggcatcggc ggcgccctcc cgtgccgggg tgcccagcag
     7021 gtcgtcgatc atcgcacgca cggcacggat ctgcggcccg cgccgggtcg catactccgc
     7081 cccttcgtag ccccaccagt cccagcagcc gagcgggttg agcggctgca tggtcggctt
     7141 gacctgcggg aacagcacga cgatgcgccc ggcctcggcc gcctcgaggt aaccgctgcg
     7201 ccgcacgaag gcgtcgccca cctcgcccgc gccctgcttg cagccgtgaa agacgacgtg
     7261 cagccggcac ggcgtcgacg tgccttcggt gcacgtcgtc ggcacgtaca gccacccctc
     7321 gtcggcgaac gacacgctgc ccgccgccgc ggcatacccg cgctggtcga acgtgcgcag
     7381 cggggccggt gcggccgcgg tcggtgccgg cgccgcgatc ggcgcgtaca ggtgctcgag
     7441 cagcgcccgc gccgcgtcat agtcgcaact cgcgacgtag ggcggttcgc cggcggcaca
     7501 cggcgcggca ccgggcgccg cgatcggcag gttgtggccg gcgccctcgc gctcgatccg
     7561 cacgaggttg gcggacctga cgccgcg
//
LOCUS       DASPFR010000256         3964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_439661, whole genome shotgun sequence.
ACCESSION   DASPFR010000256 DASPFR010000000
VERSION     DASPFR010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439661"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VD737_06775"
     CDS             <1..455
                     /locus_tag="VD737_06775"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HXW10300.1"
                     /translation="SLIPGAAHWPRFVRNRSEQFEGRVGLVEVLPTPSIFLRGMEGSV
                     LPIAVAHGEGLAEFPDDEAARHCTEGGLVSLRWVDNHGRTATTYPANPNGSPYGITGL
                     TTTDGRATIVMPHPERVYRTVQNSWHPEDWGEDGAWTRMFRNARVWAD"
     gene            complement(570..1277)
                     /locus_tag="VD737_06780"
     CDS             complement(570..1277)
                     /locus_tag="VD737_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10301.1"
                     /translation="MSVYVDSSSRLHHAQERSLALMCPHCQVYTHITPVSVPQFPELV
                     ASRPNHVGVVYRCDSCSAPIFLKFAVKMYAANRVELATTFVELERSKEKFNFTYLPED
                     VERLFREALGCYTAGHFNAFASMCRRTTQAVFHDLGENGRLRIFEHVREARELAEIDD
                     DTYAVVKKVLFGADADPSPQLPELTPNESGVLLELIKDMLYETYVRKGKLQQAMMVRR
                     FFAEETLDKITPIGRAS"
     gene            complement(1282..2004)
                     /locus_tag="VD737_06785"
     CDS             complement(1282..2004)
                     /locus_tag="VD737_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10302.1"
                     /translation="MMGPLVLIVFFGCVALLLGFSRWLAHRPWAAAGNVALALLLFTA
                     AYAWWPAALHLRTYEPIPANAVVAQVYCERTGATTFRVTLTRLPGGRMQVYEVGGDEW
                     RLDARTLVWKGRARQLGLQPGYRLERLSARYLRSARPADAAGEMRLPEGFALADLDEV
                     GEDVWSQARTGQRWADAVEPGRAYGPWRPLADKARYEVWMSHEPDALDVRLEARAAAA
                     TAARQAMRYTRPRDANVRTPQG"
     gene            complement(2030..3565)
                     /gene="hutH"
                     /locus_tag="VD737_06790"
     CDS             complement(2030..3565)
                     /gene="hutH"
                     /locus_tag="VD737_06790"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HXW10303.1"
                     /translation="MPDPTPPRSVLELDGASLTLEQLRRFESERPVVRIAAGARSGME
                     RSVAAVAATIASGRVAYGINTGFGAFANRVIPAAKTQKLQLNLVRSHACGAGDPLPAA
                     IVRRMLLLKANALVAGYSGIRPQVVDALLALLNADVLPSIPSQGSVGASGDLAPLAHM
                     TLALLGEGEATSADGRALAGVDVPAAAGLVPLELQAKEGLALLNGTQLSLALALEGLF
                     RAERLLDASVVSCAMTVEGLAGSHAPFDDRVQQVSRLPGQIAAARKIRALLDSDSEIR
                     RSHEHCERVQDPYAVRCMPQVFGAVEHTLAHARDVLAQAVNGVSDNPLIFGEDVISGG
                     NFHAEPIGFVSDFLAIAVAELASTSERRIDLLDRRVNPNLNMFLAAEPGLESGFMMAH
                     VTAAALVSENKTYAHPASVDSLPTSAGQEDHVSMAPWAGRKLLRICDNTARVLGVELL
                     AAAHALDAMRPLTTTVELQRVHAELRTIAPYRPADHRLDGDMARLAEFVESGALSAHV
                     PPG"
     gene            complement(3558..>3964)
                     /locus_tag="VD737_06795"
     CDS             complement(3558..>3964)
                     /locus_tag="VD737_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXW10304.1"
                     /translation="TPSRAYVGRFNFRGAQQQQTIGELSGGERNRVHLAKVLKEGGNV
                     ILLDEPTNDLDIETLRALEEALLSFPGCAVVISHDRWFLDRIATHILAFEGNSEVVWF
                     EGNYSEYVEDLKKRRGEDAEQPHRMRYRSLHA"
BASE COUNT          597 a         1347 c         1397 g          623 t
ORIGIN      
        1 agtcgctgat cccgggcgcc gcgcactggc cgcggttcgt ccgcaaccgc tcggaacagt
       61 tcgaaggccg cgtcggcctc gtcgaggtgc tgccgacgcc gtcgatcttc ctgcgcggga
      121 tggagggttc ggtgctgccg atcgccgtcg cgcacggcga ggggctcgcc gagtttcccg
      181 acgacgaagc tgcgcggcat tgtaccgagg gcggcctcgt gagcctccgc tgggtcgaca
      241 accacggccg cacggcgacg acctatcccg ccaacccgaa cggctcgccg tacggcatca
      301 ccggcctcac gacgaccgac ggccgcgcca ccatcgtcat gccgcacccc gaacgcgtct
      361 atcgcaccgt gcagaactcc tggcaccccg aggactgggg cgaggacggc gcgtggacgc
      421 gcatgttcag gaacgcgcgg gtgtgggctg attgaagaag tggctagtgg ctagtggcta
      481 gtggctagtg gctagtgaag aaccgcgctg tgcgcgaagc gggctctccc ttccgcacta
      541 accactagcc actgaccact gaccacttcc tatgaggcgc gtccgatcgg cgtgatcttg
      601 tcgagcgttt cctcggcgaa gaatcgtcgc accatcatgg cctgctgcag cttgcccttc
      661 cgcacgtagg tttcgtacag catgtccttg atcagctcga gcagcacgcc ggattcgttc
      721 ggcgtgagct cgggcagttg cggcgacggg tcggcgtcgg cgccgaacag cactttcttg
      781 acgaccgcgt aggtgtcgtc gtctatctcg gcgagttcac gcgcttcgcg cacgtgctcg
      841 aagatccgca ggcggccgtt ttcgcccagg tcgtgaaaga cggcctgcgt ggtacggcgg
      901 cacatcgacg cgaacgcgtt gaagtgtccg gccgtgtagc acccgagcgc ctcgcgaaac
      961 agccgctcga cgtcctcggg gaggtaagtg aagttgaact tctccttgga gcgctcgagt
     1021 tcgacgaacg tggtggcgag ctccacgcgg ttggccgcgt acatcttgac cgcgaacttc
     1081 aggaagatcg gcgcgctgca cgagtcgcac cgatagacca cgccgacgtg gttgggccgg
     1141 cttgcgacga gctccgggaa ctgcgggacc gagaccggcg tgatgtgcgt gtagacctgg
     1201 cagtgcgggc acatcagcgc gaggctgcgc tcctgggcgt ggtgcagccg gctgctcgaa
     1261 tcgacgtaga cggacatggc gtcagccttg tggggtccga acgtttgcgt cgcgaggacg
     1321 agtgtagcgc atcgcctgtc gggccgccgt ggccgcggcc gcgcgtgcct ccaggcgcac
     1381 gtcgagcgcg tccggctcgt gcgacatcca gacctcgtag cgtgccttgt cggccagcgg
     1441 acgccagggg ccgtacgcgc ggccgggttc gaccgcgtcg gcccagcgct gcccggtgcg
     1501 ggcctgcgac cagacgtctt cgccgacttc gtcgagatcc gcgagcgcga acccctcggg
     1561 cagccgcatc tccccggcgg cgtccgcggg acgggcgctg cgcaggtacc gggcgctcag
     1621 tcgttcgagc cgatagccgg gctgcagccc aagctggcgc gcgcggcctt tccagaccag
     1681 cgtgcgggca tccaggcgcc actcgtcgcc gcccacttca tagacctgca tgcggccgcc
     1741 cggcaggcgc gtcagcgtca cgcgaaaggt cgtcgcaccg gtgcgttcac agtacacctg
     1801 tgccaccacc gcgttcgccg ggatcggttc gtacgtgcgg aggtgcagcg cggccggcca
     1861 ccatgcgtac gccgcggtga acagcagcag ggcgagggcg acgttgcctg cagccgccca
     1921 gggccggtgc gcgagccagc ggctgaagcc gagcagcagc gcgacgcagc cgaaaaagac
     1981 gatcaggacg agcgggccca tcatcgacgt aggcggccgc gccgttggct cagccgggcg
     2041 gcacgtgcgc ggacagcgcg cccgactcga cgaactccgc caggcgcgcc atgtcgccgt
     2101 cgagacggtg atcggccggg cggtaggggg cgatcgtgcg cagttcggcg tgcacgcgct
     2161 gcagctcgac ggtggtcgtg agcgggcgca tcgcgtcgag cgcgtgcgcc gcggcgagca
     2221 gctcgacgcc gagcacgcgc gcggtgttgt cgcagatcct gagcagcttg cggcccgccc
     2281 acggcgccat gctcacgtgg tcttcctgtc cggccgacgt cggcaggctg tccaccgagg
     2341 ccgggtgagc gtacgtcttg ttctccgaga cgagtgctgc ggccgtgacg tgcgccatca
     2401 tgaaaccgga ctcgaggccg ggctcggcgg ccaggaacat gttgaggttg gggttcacgc
     2461 gacggtcgag caggtcgatg cgccgctcgg acgtgctcgc gagttccgcc accgcgatcg
     2521 cgaggaaatc gctgacgaag ccgatcggct cggcgtggaa gttgccgccc gaaatcacgt
     2581 cctcgccgaa tatgagcgga ttgtcggaca cgccgttgac ggcctgcgcg agcacgtcgc
     2641 gtgcgtgcgc gagcgtgtgc tcgacggcgc cgaacacctg cggcatgcag cgcacggcgt
     2701 acgggtcctg gacgcgctcg cagtgttcgt ggctgcgccg gatctcgctg tcggagtcga
     2761 gcagcgcgcg aatcttgcgc gcggcggcga tctgcccggg cagccggctc acctgctgca
     2821 cacggtcgtc gaacggcgcg tgactgccgg ccaggccctc gaccgtcatc gcgcaggaca
     2881 ccaccgaggc gtcgagcagc cgctcggcgc ggaacagccc ctcgagcgcg agcgcgaggc
     2941 tcaactgcgt gccgttcagc aacgcaaggc cttccttggc ctgcaactcc agcggtacga
     3001 gtccggctgc cgcgggcacg tccaccccgg cgagcgcccg cccgtccgcg ctcgtcgcct
     3061 cgccctcgcc cagcagggcg agcgtcatgt gggcgagcgg tgcgaggtca ccggaggcgc
     3121 cgaccgaccc ctgcgagggt atcgacggca gcacgtcggc gttgagcagt gccagcagcg
     3181 catcgacgac ctgcggccgt atgccggagt agcccgcgac cagcgcgttc gccttgagca
     3241 gcagcatgcg ccgcacgatg gccgccggca gtggatcccc ggcgccacag gcgtggctgc
     3301 gcacgaggtt gagctgcagc ttctgcgtct tcgccgccgg gatcacgcga ttggcgaacg
     3361 cgccgaatcc cgtgttgatg ccgtacgcga cgcggccgga ggcgatcgtc gcggccacgg
     3421 ccgccaccga ccgctccatg cccgatcgtg cgcccgccgc gatccgcacg acgggacgct
     3481 ccgactcgaa ccggcgcagt tgctcgagcg tgagcgaggc gccgtcgagt tcgagcacgg
     3541 accgcggtgg cgtcgggtca ggcatgcagg ctgcgatacc tcatgcggtg cggctgctcg
     3601 gcatcctcgc cacgccgctt cttgaggtcc tcgacgtact cggagtagtt gccctcgaac
     3661 cagacgactt cgctgttgcc ttcgaacgcg aggatgtgcg tggcgatgcg gtcgaggaac
     3721 cagcgatcgt gcgagatcac caccgcgcag cccgggaagc tgagcagtgc ttcttccagg
     3781 gcgcgcaaag tctcgatgtc gaggtcgttg gtcggttcgt cgagcaggat gacgttgccg
     3841 ccttccttca gcaccttcgc gaggtgcacg cggttgcgtt caccgcccga cagttcgccg
     3901 atcgtctgct gctgctgcgc gccgcggaaa ttgaagcggc ccacgtatgc gcgcgacggc
     3961 gttt
//
LOCUS       DASPFR010000257         7424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_74665, whole genome shotgun sequence.
ACCESSION   DASPFR010000257 DASPFR010000000
VERSION     DASPFR010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74665"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..897)
                     /gene="folE"
                     /locus_tag="VD737_06800"
     CDS             complement(271..897)
                     /gene="folE"
                     /locus_tag="VD737_06800"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HXW10305.1"
                     /translation="MNAVTTNKKDLALAERNGVERGDAEAAVRTLLRWAGEDPAREGL
                     LDTPRRVVEAYTDWFSGYAVDPREYLSRTFEEVGGYDEMVVLRDIEFESHCEHHMAPI
                     IGRAHVGYLPTGKVVGISKLARVVEVFARRFQVQEKMTSEIARCINDVLQPTGVGVVI
                     EAVHECMTTRGIHKRGVSMITSKMLGTFRSDARTRNEFLTFIDIRDRR"
     gene            complement(962..1567)
                     /locus_tag="VD737_06805"
     CDS             complement(962..1567)
                     /locus_tag="VD737_06805"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXW10306.1"
                     /translation="MPPATRALLLLNVGLFVAQMLTGPLLVRLFALWPPASPNFPGAP
                     SFELWQVLTYGFLHGSLTHLFFNMFALWMFGGEIERLLGTRRFVTYYLVCVVGAAVAQ
                     LLVVGGVAARPVPTVGASGGVFGLLLAFGMAFPRRRIMLLFPPIPMPAWLFVTLYGAL
                     ELYLGVTGSGQGVAHFAHLGGMAAGYALLVYWRGRPGSNPR"
     gene            1702..3174
                     /locus_tag="VD737_06810"
     CDS             1702..3174
                     /locus_tag="VD737_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXW10307.1"
                     /translation="MAACLGLLAAVPAPATNGTRVDFGALAFAPCTLAAAGQAVTLAA
                     RCASLRVPEDRSRPDARSIELAIAWVPSTSKSPAPDPVLFLAGGPGQSALESFPMVAP
                     AFRETLRQRHVILVDQRGTGGSHPLECPKAIDPDDPSALDAGDPDTARRIAERCLDEV
                     RGNADPRFYTTSTYVADLEAVRAALGAPRVNLVGVSYGTRVALEYLRRHAAHVRAVVL
                     DSTVPPPLILGAEHARNLDAAIDAQFRRCEADAACRERFGSPRERLGSLLERLRAQPV
                     KVAYADPVTDEPREDELTAEALAGVVRLHAYSPQLFAMLPMLLEGAANGRYQHLMAQA
                     RMVEQLVGEQISVPLQLAVSCTEDAPWLVGDPADRDTLLGTAFVDFLRAQCEVWPRGG
                     MPEDFHTPVVADRPVLLFSGEFDPVTPPRYGAEVARRLPDARHLVLRGQGHGVLGIGC
                     TPRLLAQFLERPESARNLDAHCLDALGYVPPFAGAYGWDP"
     gene            3191..3937
                     /locus_tag="VD737_06815"
     CDS             3191..3937
                     /locus_tag="VD737_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXW10308.1"
                     /translation="MIRVEGLRKAFRTRGSVVQAVDGLDFEAADGQITGLLGPNGAGK
                     TTTLRMLYTLMAPEAGRVLVDGLDAAVEPARVRATLGVLPDARGLYKRLTARENVAYF
                     GELHGLERPVIHARTSHLCAALDMHEFIDRRTEGFSHGQRTKTAIARALIHDPRNVVL
                     DEPTNGLDVMTTRALRRFLLGLRDDGRCVLFSSHIMQEVAQLCDRIVVIAHGRVVASG
                     SPAELRESTGCENLEDAFVKAIGSEEGLYA"
     gene            3934..5112
                     /locus_tag="VD737_06820"
     CDS             3934..5112
                     /locus_tag="VD737_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXW10309.1"
                     /translation="MKALWVVLRKELLDAFRDRRMVAVAFLVMPLAVPLVLAGTAALG
                     ARKQAAQFESTLRLPVAGAEHAPNLIDWLEQHNLEVVTAPPDPDAAVRTQRHDVILRI
                     DARYAEDWRAGRPAGVEVIFDSSRPMASGATIARVRGLLDGYSDTVGTLRLIARGIHP
                     AVARPLLVGTRDVATPEARFDLTQQLLPYLLLLLAFIGGMQLAIDATAGERERQSLEP
                     LLATPASREAIISAKILATAVFALITIAVTLLAYRVSFTVLPDDFAGTDFALGPGALA
                     QLLVVILPVVLLGATVLTALAAYARSYREAQGYLPLLIFLPMVPTLVLMVAPVRTQAW
                     MLAVPFLGQNQLILKILRSETVAPGEWALSLGAGVALVALAWLVAARLYHREHLAASS
                     "
     gene            complement(5062..5865)
                     /locus_tag="VD737_06825"
     CDS             complement(5062..5865)
                     /locus_tag="VD737_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HXW10310.1"
                     /translation="MYERLLLPIAIAQGFWLLYRTPKLPAPVGDSGRAGPGFVPALKL
                     AGVGDSIMVGTGVREQCHSLTATYARLLHARLGCSVDWRVHGRNGATSGQVLREVLPT
                     VQRAHVYLLSCGVNDATHQVPVDEFARNLGAILDALRDRSPRAAILYGGLPPLETFPA
                     LRWPLKAVLAERVRQMRAAAAEVIARHRRVFHFQFPPAMSPEHFASDGFHPHEQSCEQ
                     WASGLLALWPPTLDVGAGRAASGGRRRRRSALGSGRGGQMLPVVQPGSD"
     gene            complement(6060..>7424)
                     /locus_tag="VD737_06830"
     CDS             complement(6060..>7424)
                     /locus_tag="VD737_06830"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXW10311.1"
                     /translation="DAETVANIEHWREKLKEWPYTKAVMLPHVGQAREVARPGEIFRQ
                     PDLAATLRKLVEAEKQALAAGKSRKEAIQAAYDRFYRGDVAQEFVRGVQEQGGLITLA
                     DLANWKPKIEEPLSVNYRGIDVYKLDVWTQGPALLQSLNILENFDLRTMGYNSANYVH
                     TVYQAMNLAFADRDFYYGDPAFPPAEPTQGLLSKAYAKQRASAIRADRNDPAAGPGDP
                     YPFQGARNPFADLLAKQRRAALERLDAPELTAQQRADFEAAFRAGTTSQVAADADGWL
                     VAVTPSGGWVPAVIAGRTGIGLSQRAQSFVLDPAENPYNVIEPGKRPRVTLTPSLALR
                     DGKPWLAFAVQGGDTQDQNLLQFFLNVVEFGMTPQEAVEAANFNSYQMKASFDLHESQ
                     PGRIVLNASMPQWVRRELALRGYTMESEQRTSGPINAILVDPENGSFWGGSSNHGEDY
                     GIGW"
BASE COUNT         1064 a         2606 c         2625 g         1129 t
ORIGIN      
        1 gccccaggtc gtacgagccc acgacgaggc agccgcccca gcgccgccag ttgtgcggca
       61 ggcacagctg cgacgtccgt tcgtggaaag gcgtgcgcct gagctggtag cgcgggtgat
      121 ggagggcgga gggcgccggc atcgggcgaa tatagcggaa agcggatagc ggttaggggt
      181 tagcggttag cggttagtcg gagcgcaata gatgggccgg cactccagca gcttcttact
      241 aaccgctaac cgctaaccgc cttccgcttc ctaccttcgg tcgcggatgt cgatgaacgt
      301 caggaattcg ttgcgggtgc gggcgtccga acggaacgtc ccgagcatct tcgaggtgat
      361 catggagacg ccgcgcttgt ggatgccgcg cgtggtcatg cactcgtgca cggcctcgat
      421 caccacgcca acgccggtcg gctgcagcac gtcgttgatg caacgggcga tctcggacgt
      481 catcttctcc tgcacctgga accgtcgcgc gaacacctcg acgactcgcg cgagcttgga
      541 gatgccgacg accttgcccg tgggcaggta tcccacgtgc gcgcgaccga tgatcggcgc
      601 catgtggtgc tcgcagtggg actcgaactc gatgtcgcgc agtacgacca tctcgtcgta
      661 gccgcccact tcctcgaagg tccggctcag gtattcgcgc gggtccaccg cgtagccgga
      721 aaaccagtcc gtgtacgcct ccaccacgcg ccggggcgtg tcgagcaggc cctcgcgggc
      781 cgggtcctcg ccggcccagc gcagcagcgt gcggacggcg gcttcggcgt caccgcgttc
      841 cacgccattg cgctcggcca gcgcgagatc tttcttgttc gtcgtgacgg cgttcatgtg
      901 aggccgtttt cggcgggcgc tcgacttcgg atgcacggcc atcgtcactc tccttgcttg
      961 ctcagcgcgg attcgatccg ggtcggccgc gccagtacac cagcaaagcg tacccggccg
     1021 ccatgccgcc gaggtgggcg aagtgcgcga cgccctgccc ggagcccgtg acgccgagat
     1081 agagttccag ggcgccgtac agcgtcacga agagccaggc cggcatcggg atcggtggaa
     1141 acagcagcat gatgcgccgt cgcgggaagg ccatgccgaa cgccagcaac agcccgaaca
     1201 cgccgcccga cgcgccgacg gtcggtactg gacgcgcggc cacgccgccc accacgagca
     1261 gttgcgccac cgccgcgccc acgacgcaca cgaggtagta ggtgacgaac cgccgcgtcc
     1321 cgagcaggcg ctcgatttcg ccgccgaaca tccagagcgc gaacatgttg aagaacaggt
     1381 gcgtgaggct gccgtgcagg aagccgtacg tcagcacctg ccacaactcg aaagacggag
     1441 cacccgggaa attgggcgat gccggcggcc acaacgcgaa caaccggacc agcaggggcc
     1501 cggtgagcat ctgcgcgacg aaaagcccga cgttgagcag cagcaacgcc cgggtggcgg
     1561 ggggcatgga cgatcgcatc cggccatcgt agcggaatcg tgccggcgcg gacgccgtcc
     1621 ggccgtgcta gggtggtgtc ggacacgatg accacggcgc cccgcatctc atcgagcacg
     1681 cgcgtctccc gagccggccg aatggccgcg tgcctcggcc tgctcgccgc cgtgccggcg
     1741 cccgccacca acggaacgcg cgtggacttc ggcgcgctgg ctttcgcgcc ctgcacgctc
     1801 gccgcggcgg ggcaagccgt gacgctggca gcgcgctgcg cgtcgctgcg ggtgcccgaa
     1861 gaccgctcgc gcccggacgc gcggagcatc gaactcgcga tcgcctgggt gcccagcacc
     1921 tcgaagtcgc ccgcgcccga tccggtgctg ttcctggccg gcgggccggg ccagtccgcg
     1981 ctcgagtcgt ttccgatggt cgcccccgcg tttcgtgaaa ctctgcgcca gcgccacgtg
     2041 atcctggtgg accagcgtgg gaccggtgga tcgcatccac tcgagtgccc gaaggccatc
     2101 gatccggacg atccgtctgc gctggatgcg ggcgatccgg acaccgcgcg caggatcgcc
     2161 gagcggtgcc tggacgaggt ccgcggcaac gccgaccctc gcttctacac cacgtcgacc
     2221 tacgtggccg acctcgaggc ggtccgcgcc gcgctcgggg ctccgcgcgt gaatctggtc
     2281 ggcgtctcgt acggcacgcg cgtcgcgctc gagtacctgc gtcgccacgc ggcgcacgtc
     2341 cgcgccgtgg tgctcgattc gacggtgccg ccgccgctga tcctcggcgc ggagcacgcg
     2401 cgcaacctcg acgcggcgat cgacgcacag ttccgccgtt gcgaggccga cgcagcctgc
     2461 cgcgagcgct tcggatcacc gcgcgagcgt ctcgggtcgc tgctcgagcg gctgcgcgcg
     2521 cagccggtca aggtggccta cgccgacccc gtcaccgacg agccacggga ggacgagctg
     2581 accgccgaag ccctggcggg cgtcgtgcgc ctgcatgcgt actcgccgca gctgttcgcg
     2641 atgctgccga tgctgctcga gggggccgcg aacggccggt accagcacct gatggcgcag
     2701 gcccgcatgg tcgagcaact ggtcggcgag cagatctcgg tgccgctgca gctcgcggtg
     2761 agctgtaccg aggacgcgcc gtggctcgtc ggcgacccgg cggatcgcga cacgctgctc
     2821 ggcaccgcct tcgtcgactt cctgcgcgcg cagtgcgagg tctggccgcg cggcgggatg
     2881 cccgaggact ttcacacacc tgtcgtcgcc gaccggccgg tgctgctgtt ctccggcgag
     2941 ttcgatccgg tgacgccacc gcgctacggc gcggaggtcg cgcgccggtt gccggacgca
     3001 cgtcatctcg tgctgcgggg acagggccac ggcgtgctgg gcatcggctg cacgccgcgc
     3061 ctcctcgcgc agttcctcga acgtccggag tccgcgcgca atctcgacgc acactgcctc
     3121 gacgcgctcg gctacgtgcc gccgttcgca ggcgcgtacg gctgggatcc gtgacggacg
     3181 gccgcgcgcg atgatccgcg tcgaaggcct gcgcaaggcg ttccgcacgc gcggcagtgt
     3241 cgtccaggcc gtggacgggc tcgatttcga ggcggctgat ggccagatca ctggcctgct
     3301 cgggccgaac ggcgcgggca agacgacgac gctccgcatg ctctacacgc tgatggcgcc
     3361 agaggccggc cgcgtgctgg tcgacggcct cgacgccgcg gtcgagccgg cacgcgtgcg
     3421 cgcgacgctc ggcgtgcttc ccgacgcccg ggggctctac aagcggctga cggcgcggga
     3481 gaacgtcgcg tacttcggcg agctgcacgg gctggagcgc ccggtgatcc acgctcggac
     3541 ttcgcacctc tgcgcggcgc tcgacatgca cgagttcatc gaccggcgca ccgaaggctt
     3601 ctcgcacggc cagcgcacca agaccgcgat cgcgcgggcg ctgatccacg atccgcgcaa
     3661 cgtggtgctc gacgagccca ccaacggcct ggacgtgatg accacgcggg cgttgcgccg
     3721 gttcctgctc ggcctgcgcg acgacggccg ctgcgtgctg ttctcgagcc acatcatgca
     3781 ggaggtggcg cagctctgtg atcgcatcgt ggtgatcgcg cacggccgcg tcgtcgcttc
     3841 cggatcgccg gccgagctgc gcgagagcac cggctgcgag aacctcgagg acgccttcgt
     3901 caaggcgatc ggcagcgagg agggactcta cgcgtgaagg cgctctgggt cgtgctgcgc
     3961 aaggagctgc tcgacgcctt ccgcgaccgt cgcatggtcg cggtcgcgtt cctggtcatg
     4021 ccgctcgccg tgccgctggt cctcgccggc acggccgcgc tcggcgcgcg caagcaggcg
     4081 gcgcagttcg agtcgacgct gcgcttgccg gtggcagggg ccgaacacgc cccgaacctg
     4141 atcgactggc tcgagcagca caacctcgag gtggtgacgg cgccacccga ccctgacgct
     4201 gccgtgcgca cgcaacggca cgacgtgatc ctgcgcatcg acgcgcggta cgccgaggac
     4261 tggcgcgccg gtcgcccggc gggcgtcgag gtgatcttcg acagttcgcg gccgatggcg
     4321 agcggcgcga cgattgcccg ggtgcgcggg ctgctggacg gatactcgga cacggtgggc
     4381 accttgaggc tgatcgcgcg cggcatccac ccggcggtcg cacggccgtt gctggtcggc
     4441 acccgggacg tcgcgacgcc cgaggcgcgc ttcgacctca cgcagcaact gctgccgtac
     4501 ctgttgctgc tgctcgcctt catcggcggc atgcaactgg cgatcgacgc cacggccggc
     4561 gagcgcgagc gccaatcgct ggagccgctg ctcgcgacgc ccgcgtcgcg cgaggcgatc
     4621 atcagcgcca agatcctcgc gaccgcggtg ttcgcgctga tcacgatcgc cgtgacgctg
     4681 ctggcctacc gcgtgtcgtt cacggtgctg ccggacgact tcgccggcac ggacttcgcc
     4741 ctgggacccg gtgcgctcgc gcagctgctc gtcgtgatcc tgccggtcgt gctgctcggg
     4801 gcgacggtgc tcacggcgct cgcggcctat gcgcgcagct accgtgaagc gcagggctac
     4861 ctgccgctgc tgatcttcct gccgatggtg ccgacgctcg tgctgatggt cgcgcccgtg
     4921 cggacgcaag cctggatgct ggcggtgccg ttcctcggcc agaaccagct gatcctgaaa
     4981 atcctgcgat cggagaccgt cgcgccgggc gagtgggccc tgagcctggg cgcgggggtc
     5041 gcgctggtgg cgctcgcgtg gctagtcgct gcccggctgt accaccggga gcatctggcc
     5101 gcctcgtcct gaacccaggg cggaccggcg cctgcgccgc ccgccagagg ccgcccggcc
     5161 tgcaccgacg tcgagcgtcg gcggccatag cgccagcagg ccggaggccc actgctcgca
     5221 cgactgctcg tgcggatgga acccgtcgct ggcgaagtgt tcgggcgaca tggcgggcgg
     5281 gaactggaaa tggaagacgc gcctgtgccg ggcgatcact tcggcggccg ccgcgcgcat
     5341 ctggcgcacg cgctcggcca gcacggcctt gagcggccag cgcaacgccg ggaacgtctc
     5401 gagcggcggc agcccgccgt acaggatcgc cgcgcgcggg gaccggtcgc gcagcgcgtc
     5461 gaggatcgca cccaggttcc gcgcgaactc gtcgaccggc acctggtgcg tggcgtcgtt
     5521 gaccccgcac gagaggaggt agacgtgggc tcgctgcacg gtcggcagta cctcgcgcag
     5581 cacctggccc gaggtggccc cgttgcggcc gtgcaccctc cagtcgaccg aacagccgag
     5641 ccgcgcgtgc agcaggcgcg cgtacgtggc cgtgagcgag tggcactgct cccgcacccc
     5701 ggtgccgacc atgatggagt ccccgacgcc ggcgagcttg agggccggca cgaagcctgg
     5761 gccggcacgt cccgagtcgc cgacgggcgc gggcagcttc ggcgtccggt acagcagcca
     5821 gaacccctgg gcgattgcga tcggcagcag cagcctctcg tacatgggcg ctgagcgtag
     5881 cgatcgccgc ggcctcgccc aaggcgtgaa cggctactcc agcgaaccgc gccatcgcat
     5941 cgagcggtga gaagccgatg acggcagcgg cgaacgccgg cccgggatga actggcgtcg
     6001 ggcgccaccc gccgccctac gtcggcgggc gtgcgcgctt gcacccgcgt cagcgggcgc
     6061 taccagccga tgccgtaatc ttcgccgtga ttgctcgagc caccccagaa gctgccgttc
     6121 tcggggtcca ccaggatcgc gttgatcggg ccggaggtgc gctgctcgga ctccatcgtg
     6181 tagccgcgca aggcgagctc gcgacggacc cactgtggca tcgaggcatt cagcacgatc
     6241 cggcccggct gcgactcgtg caggtcgaac gaggctttca tctggtagct gttgaaattg
     6301 gccgcttcga ccgcttcctg tggcgtcatg ccgaactcga ccacgttcag gaagaactgc
     6361 agcaggttct ggtcctgggt gtcgccaccc tgcacggcga acgcgagcca tggcttgccg
     6421 tcccgcaggg ccaggctcgg tgtcagcgtg acgcgcggac gcttgccggg ctcgatcacg
     6481 ttgtacgggt tctcggcggg atcgagcacg aacgactgtg cgcgctggct cagcccgatg
     6541 ccggtgcgtc ctgcgatcac ggccgggacc cagcctccgc tcggcgtgac ggcgaccagc
     6601 cagccgtcgg catcggccgc cacctgcgag gtggtgcccg cgcgaaacgc ggcctcgaag
     6661 tccgcgcgct gctgcgccgt gagttcgggt gcgtcgaggc gctcgagcgc cgcgcgccgc
     6721 tgtttcgcca gcaggtccgc gaacggattc ctggcgccct ggaacggata agggtcgccc
     6781 gggcccgcgg cggggtcgtt gcggtcggcc cggatcgccg atgcgcgttg tttcgcatac
     6841 gccttcgaga gcagtccctg cgtcggctcg gcgggcggga acgccgggtc gccgtaatag
     6901 aagtcgcggt cggcgaacgc gaggttcatc gcctggtaca ccgtgtgcac gtagttggcg
     6961 ctgttgtagc ccatcgtgcg caggtcgaaa ttctcgagga tgttgagcga ctgcagcagc
     7021 gccgggccct gcgtccacac gtcgagcttg tacacgtcga tgccgcggta gttgacgctg
     7081 agcggctcct cgatcttcgg cttccagttg gcgaggtcgg cgagcgtgat gagcccgccc
     7141 tgttcctgca cgccgcgcac gaactcctgc gcgacgtcgc cgcgatagaa gcggtcgtag
     7201 gccgcctgga tcgcctcctt gcgcgacttg ccggcggcga gcgcctgttt ctcggcttcg
     7261 acgagcttgc gcagcgtcgc ggcgaggtcc ggctggcgga agatctcgcc cgggcgcgcg
     7321 acctcccgcg cctggccgac gtgcggcagc atgaccgcct tcgtgtacgg ccactccttg
     7381 agcttctcgc gccagtgctc gatgttggcc acggtctcgg cgtc
//
LOCUS       DASPFR010000258         4422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1173431, whole genome shotgun sequence.
ACCESSION   DASPFR010000258 DASPFR010000000
VERSION     DASPFR010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173431"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..2080
                     /locus_tag="VD737_06835"
     CDS             371..2080
                     /locus_tag="VD737_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10312.1"
                     /translation="MLVLAPFVLLALAETGLRIAGFGRDLEPLFIPSPQQPGYLQANP
                     RAVLRLFVDPGQAPPVSIETAYFRAEKPPGTFRVFVQGESSAAGFPYGLGASLAGVLD
                     QRLERAFPSREVEVISTALAAVNSYALVDFADEILAQQPDAVVVYVGHNEYFGVLGAG
                     STLRMAGSPTLTRAYLAVRDWRLFQLVARLIGLTGRRADVPVASGDDTLMAGVAGDRT
                     IPLNSALFRRGEEQFGANLDRLLARYAEAGVPVFIGTLISNERDQPPLAVLAGLEGDA
                     TGAAKMAYDTAQDADGAAKYEAARDGYAWARDLDPLRFRAPSVFSSIVAVQARRHGAT
                     LVDVHRRFVEASPHGLVGDDLLLEHVHPNLDGYFLLADAYFDAMLAASVPGPPEVVID
                     DAQARQEMPVTDVDRWLGDYKVRRIESAWPFTTESVRPDLPPPVTEGERLAQALYQER
                     LSWPEAQDALRRHYRATGNKPELARSTTVLADAFPFAATLQFEAAAALIDVGRPADGW
                     RYAERAIALEPRTLNHLLVGAHALLSSGRRDEGRALLERVLGIDPGNATARRVLQELA
                     ATP"
     gene            2077..2808
                     /locus_tag="VD737_06840"
     CDS             2077..2808
                     /locus_tag="VD737_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10313.1"
                     /translation="MKIVKQSERYIVFDDVLPPEQFQLLVEYAARNEYARNPKWIKPW
                     DLADEMPWQTGETVYTRGKALDAQDPRRAYPTGMAIDLLLRKILDSRFYRDRVPDTYV
                     TARTYLHPPGSGLDWHDDEGRFAGAYTYYVHPRWHASWGGELLVVEDVLPRSGLPVKR
                     HMREGRIDTVTGTWDVLTKDDLSEMLMNGATSTEFVFAKPNRLVFLQGGVWHRINVVK
                     RNAPFARAAIAGFFLPETDATTIWR"
     gene            complement(2812..>4422)
                     /locus_tag="VD737_06845"
     CDS             complement(2812..>4422)
                     /locus_tag="VD737_06845"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXW10314.1"
                     /translation="QPNADAIRRFFPDPARAPDVSIDTTWFPAAKAPATLRIFVQGES
                     SAAGFPYGRWASPAALLQQRLQRAYPDRNVEVINTAMAAVTSYVLLDFADEIIAQQPD
                     AVVIYAGHNEFLGIGGVGSSLVSARSPTVARAIASLRRLHVYRGLERALGPMLGPKED
                     PLAGRGGTLMARVARERSIALGSDLYSRGERQFRGNLERLLGKYQRAGVPVFIGTLAS
                     NLRDQPPFVSVVGSGPQSAQVRYQAAQALDRAGRHESALEEYILARDLDGLRFRAPSS
                     FEAILREVAARHGAIVVEVGQAFASAARDGIVGADLMLEHVHPNVEGYFQLASAFYRS
                     LAEWAGPPAVPVDDVQARQESPVTEVDRLAGDYRVLLLRNDWPFVPERRAVTLPPATT
                     PIERIAQAWFAGRLSWADAMRNAIDTYQAEGNGVEAARVAVNLADAFVTSDAMQYTAG
                     RLLLRAGQPERALRYLERASALDAAGAIVEYRLSLAEALARTGALDRSISTLETILRR
                     HPNDERAKYWLQEMRLRQQDSVGAARAP"
BASE COUNT          639 a         1586 c         1557 g          640 t
ORIGIN      
        1 ggtacttacg gtgggtgcgc cgctcacgct cgcctgcgcg gcgccgggca atggcaacat
       61 cgcgttcacg gcgccggccg ccgggctcta cgagttcacg ctcaacgcga cgaacccgac
      121 ggcaccgcaa ctgacggtaa ccggcccctg agcaggcacc cggggcgcgt caggtgacgc
      181 gcgccgggcg acaggcgaaa cttctaagcc tggcacctcg cggtgcccgg cttttttctc
      241 gctgaagcgc caagctcact tgtccagtca ccgccatcgt cgacgggatc cgtcgcccga
      301 acccggttcc agcgatccgc cgccgcccgc gccggcggcg tcgcgccgcc gcctgtgggc
      361 gttccgagcc gtgctcgtcc tggcaccgtt cgtgctgctc gcgctcgccg agaccggact
      421 gcgcattgcc ggcttcggcc gcgacctcga accgctcttc atcccgagcc cgcagcagcc
      481 gggctacctg caggcgaacc cgcgggccgt gctgcggctg ttcgtggatc cgggccaggc
      541 gcccccggtc agcatcgaga ccgcgtattt ccgcgccgag aagccacccg gcacgttccg
      601 ggtgttcgtg cagggcgagt cgagcgcggc cggattcccg tacggcctcg gggcctcgct
      661 cgcgggcgtg ctggaccagc gcctggagcg cgccttcccg tcgcgcgagg tcgaggtgat
      721 ctcgaccgcg ctcgccgccg tgaactccta tgcgctggtc gatttcgccg acgagatcct
      781 cgcgcagcag cccgacgccg tcgtcgtcta cgtgggccac aacgagtatt tcggcgtgct
      841 cggcgccggc tcgacgctgc gcatggccgg ctcgccgacg ctcacgcgcg cgtacctggc
      901 cgtgcgcgac tggcgcctgt tccagctcgt ggccaggctg atcgggctga ccgggcggcg
      961 tgcggacgtg ccggtggcga gcggcgacga cacgctgatg gcgggcgtcg ccggcgaccg
     1021 gaccataccg ctcaattcgg cgttgttccg gcgcggcgag gaacagttcg gtgccaacct
     1081 ggaccggctg ctcgcgcggt acgccgaagc gggcgtgccg gtgttcatcg gcacgctgat
     1141 cagcaacgag cgcgaccagc ccccgctggc ggtgctggcc ggcctcgagg gcgacgcgac
     1201 gggcgcggcg aagatggcct acgacacggc ccaggatgcg gacggcgccg ccaagtacga
     1261 ggctgcgcgc gacggttatg cctgggcgcg cgacctcgat ccgctgcggt tccgcgcgcc
     1321 gtccgtcttc agctcgatcg tcgccgtgca ggcccgccgg catggcgcaa cgctggtcga
     1381 cgtgcaccgc cggttcgtcg aggcgagccc gcacggcctc gtcggcgacg acctgctgct
     1441 cgagcacgta cacccgaacc tggacggcta cttcctgctt gccgacgcct acttcgacgc
     1501 gatgctcgcg gccagcgtgc ccgggccccc ggaggtcgtg atcgacgacg cacaggcgcg
     1561 ccaggaaatg ccggtcaccg acgtcgaccg ttggctcggt gactacaagg tgcgtcgcat
     1621 cgagtcggcc tggcccttca ccaccgaaag tgtccggccc gacttgccgc cgcccgtgac
     1681 ggaaggcgag cgactcgcgc aggcgctgta tcaggaacgg ctgagctggc cggaggcgca
     1741 ggacgccctg cgacggcact accgcgcgac cggcaacaag cccgaactcg cccgcagcac
     1801 gaccgtgctg gccgacgcgt tcccgttcgc ggccacgctg cagttcgaag ccgccgcggc
     1861 gctgatcgac gtcggtcggc ccgcggacgg ctggcgctat gccgagcgcg ccatcgcgct
     1921 cgagccgcgc accctgaacc acctgctggt cggggcgcac gcgctgttgt cgagcggccg
     1981 gcgcgacgaa ggccgcgcgt tgctcgaacg cgtgctcggc atcgatcccg gcaacgccac
     2041 cgcgcgccgc gtactgcagg agctggccgc cacgccatga agatcgtcaa gcagtccgaa
     2101 cgctacatcg tcttcgacga cgtgctgcca ccggagcagt tccagctgct ggtcgagtac
     2161 gcggcgcgca acgagtacgc gcgcaacccg aagtggatca agccgtggga cctcgccgac
     2221 gagatgccgt ggcagacggg ggagacggtt tacacgcgtg gcaaggcgct ggacgcgcag
     2281 gatccgcgcc gcgcctatcc gacgggcatg gcgatcgacc tgctgctgcg caagatcctc
     2341 gactcacggt tctatcgcga ccgggtgccg gacacttacg tgacggctcg cacgtacctg
     2401 cacccgcccg gctcgggcct cgactggcac gacgacgagg ggcggtttgc gggcgcgtac
     2461 acgtactacg tgcacccgcg ctggcacgcc tcctggggcg gcgaactgct ggtcgtcgaa
     2521 gacgtgctac cgcgctcagg cctgcccgtg aagcgtcaca tgcgcgaggg gcgcatcgac
     2581 acggtgaccg gaacctggga cgtgctgacc aaggacgacc tgtcggaaat gctcatgaac
     2641 ggtgccacca gcaccgagtt cgtgttcgcg aagcccaatc ggctcgtgtt cctgcagggc
     2701 ggcgtctggc accggatcaa cgtggtcaag cgcaatgcgc cgttcgcgcg ggcggcgatc
     2761 gccgggttct tcctgcctga gacggacgcg acgacgatct ggcggtgagg gtcagggcgc
     2821 acgcgcagcg cccacggaat cctgctgccg cagtcgcatc tcctgcagcc agtatttcgc
     2881 gcgctcgtcg ttcgggtgcc tgcggaggat cgtctccagc gtcgagatcg agcggtccag
     2941 ggcgccggtg cgcgcgagcg cctcggccag cgacaggcgg tactcgacga tcgcgcccgc
     3001 agcatcgagc gccgacgcgc gctcgaggta gcgcaacgcg cgctctggtt gtccggcccg
     3061 cagcagcagg cgccccgccg tgtactgcat cgcgtccgag gtcacgaacg cgtcggccag
     3121 gttgaccgcg acgcgcgccg cctcgacgcc gttgccctcg gcctggtacg tgtcgatcgc
     3181 gttgcgcatc gcgtctgccc acgacaagcg gcccgcaaac cacgcctgcg cgatgcgctc
     3241 gatcggggtc gtcgcgggcg gcagggtcac ggcacggcgc tcgggcacga acggccagtc
     3301 gttcctgagc agcagcacgc ggtagtcccc ggcgagccgg tcgacctcgg tcacgggcga
     3361 ttcctgccgc gcctgtacgt cgtcgacggg cacggcaggc ggccccgccc attcggcgag
     3421 cgagcgatag aacgcgctgg cgagctggaa atacccttcg acgttggggt gcacgtgctc
     3481 gagcatcagg tccgcgccga cgatgccgtc gcgcgcagcg ctcgcaaagg cctgcccgac
     3541 ttcgacgacg atcgcgccgt ggcgcgcggc gacctcgcgg aggatcgcct cgaacgagga
     3601 cggcgcgcga aaccgcaggc cgtcgaggtc tctcgccagg atgtactcct cgagcgccga
     3661 ctcgtggcgc ccggcgcggt cgagtgcctg cgcggcctgg tagcggacct gcgcggactg
     3721 cggaccgctc cccaccacgc tgacgaacgg cggctggtcg cgcaggttgc tggcgagcgt
     3781 gccgatgaag accggcacgc cggcccgctg gtacttgccg agcagccgct cgaggttgcc
     3841 gcggaactgc cgttcgcccc gtgagtacag gtccgagccg agggcaatcg agcgttcacg
     3901 cgccacgcgc gccatgagcg tcccgccgcg tcccgccagc ggatcttcct tcggcccgag
     3961 catcggcccg agtgcgcgct ccaggccgcg gtagacgtgc aggcgccgca acgaggcgat
     4021 cgcgcgggcc acggtcggcg atcgcgcgct cacgagcgag gagcccacgc cgccgatgcc
     4081 gaggaactcg ttgtgtcccg cgtagatcac gaccgcgtcc ggctgctgcg cgatgatctc
     4141 gtcggcgaaa tcgagcagca cgtagctcgt gacggcggcc atcgcggtgt tgatcacctc
     4201 gacgttgcgg tcgggatagg cacgctgcag gcgttgctgc agcagcgcgg cgggcgacgc
     4261 ccagcggccg tacgggaacc cggccgcgct cgattcgccc tgcacgaaga tccgcagcgt
     4321 tgcgggcgcc ttcgccgccg ggaaccacgt cgtgtcgatg ctgacgtcgg gcgcgcgtgc
     4381 aggatcaggg aagaagcgcc ggatcgcgtc cgcgttcggt tg
//
LOCUS       DASPFR010000259         8356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_562789, whole genome shotgun sequence.
ACCESSION   DASPFR010000259 DASPFR010000000
VERSION     DASPFR010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8356
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562789"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /gene="lepA"
                     /locus_tag="VD737_06850"
     CDS             <1..1100
                     /gene="lepA"
                     /locus_tag="VD737_06850"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HXW10315.1"
                     /translation="VDKLGRFTPKSIVTASLETGEVGFVVAGIKEIDGAPVGDTITLE
                     SRPCTEPLPGFKAIKPRVFAGLFPTSSDDYEAFRDALQKLKLNDSALHYEPEVSTALG
                     FGFRCGFLGLLHMDIVQERLEREYELNLVTSAPTVVYEVVTTDGDVVNVDNPAKLPPP
                     DKVAELREPIIVANILLPPDYVGPVMKLCLEKRGVQKKLLYLGNQVSMQWELPLAEVV
                     LDFFDRLKSVSRGYASFDYEFDRFQGAPLVKLDILINEEKVDALSIISHRDSAYQRGR
                     ELVDKMQELIPRQMFEVAIQAAIGAHVIARATVKALRKNVLAKCYGGDVSRKRKLLEK
                     QKEGKKRMKQLGSVEIPQEAFLAVLQVGRKD"
     gene            1186..1971
                     /gene="lepB"
                     /locus_tag="VD737_06855"
     CDS             1186..1971
                     /gene="lepB"
                     /locus_tag="VD737_06855"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXW10316.1"
                     /translation="MVFDFSFILVVATLVTGLIWAADAWVLKPQRVVAANGDVAALRE
                     PVVVEYARSFFPIILIVLVIRSFLFEPFRIPSDSMMPTLLDGDFIFVNKFTYGLRLPV
                     VNTEVVALGEPQRGDVVVFRLPSDPSTNYIKRLVGLPGDHVVVRDKRVYINGELQPVE
                     LDGMSDPYGSTPAAQIGKERLGAVEHRTLYIAERPSVDFNDVVPAGHYFFMGDNRDNS
                     RDSRFPEVGFVPADHVVGKAVRIWLNWNLPAAPLWGRIGEAIH"
     gene            2004..2369
                     /locus_tag="VD737_06860"
     CDS             2004..2369
                     /locus_tag="VD737_06860"
                     /inference="COORDINATES: protein motif:HMM:NF027463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HXW10317.1"
                     /translation="MKHRQRGATFLGIVVILLILGAALYAGIRLVPVYLEYTKVARAL
                     EQVRDENAAVDTNSQQIRNALERRWDVEDIKRIGWKEIDIAKTTDGFDVSASYEAEEP
                     FIANVYLLVKFDKTVTIPQ"
     gene            2385..3074
                     /gene="rnc"
                     /locus_tag="VD737_06865"
     CDS             2385..3074
                     /gene="rnc"
                     /locus_tag="VD737_06865"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HXW10318.1"
                     /translation="MRDAGEWAARRLGYRFGDPALLEAALTHRSASRTNYERLEFLGD
                     SVLNFVVALLAFRAFPEAPEGDLSRFRAALVSAPSLAAVAGALELGEQLRLGSGELKS
                     GGFRRDSILADALEAVIGAIYLDGGLDAASATVARLLGGKLADLPSAETLKDPKTRLQ
                     EQLQSRGLALPRYALEEIGGEPHEQWFVASCEAPGLGLRFVGRGSSRRRAEQEAAQKV
                     LDALGHGEESA"
     gene            3071..4000
                     /gene="era"
                     /locus_tag="VD737_06870"
     CDS             3071..4000
                     /gene="era"
                     /locus_tag="VD737_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HXW10319.1"
                     /translation="MKDETGQGPQERPIRCGFAALAGRPNVGKSTLLNTLLGRKVSIV
                     SPKPQTTRTRIHGVLNRPDLQITFVDLPGIHAKQTRAMNRYMNRTAISSLSDVDLNLF
                     VVEALRWTDEDERVLAALKQAGLPIVLVINKVDRAQPRERLLPYIEQLAQKAEFAEIV
                     PVAATRPASLEALPGILAKHLPEQPRIFPEDQVTDSPDHFVAAELIREKLMLRLQEEL
                     PYGLVVEIEGMGDSEDEPGKLFVQAVIWVERAGQKAIVIGKGGELLKTIGRQARLDLK
                     EHFGRAVHLELWVKVKEGWSDDENAMRKFGYES"
     gene            3997..4851
                     /locus_tag="VD737_06875"
     CDS             3997..4851
                     /locus_tag="VD737_06875"
                     /inference="COORDINATES: protein motif:HMM:NF014607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HXW10320.1"
                     /translation="MSSARRVSLEPAFLLHHYPWRDTSRILELLTRTHGRVSVVARGS
                     RQSAATTGGSLQSFTACLVSWSGRGEMGYLTGVERLLRSSPTSAGEVARSAGEGSGSP
                     EPSPDLRSTSPGVPGEVKSLAGDRLMSGFYANELLLRLLQRHDPHPALFDAYATLLER
                     LRAADEDAARALRIFEKRLLEELGWGLNLEHEAASGAPVEASRSYRYGLEGGAEPIDG
                     VAAGSLIFCGASLLSLAGETLDDPRSLADARRLLRAALDRLLDGRALRTRDVLLEMRA
                     HAGHTQGV"
     gene            4854..5612
                     /gene="pdxJ"
                     /locus_tag="VD737_06880"
     CDS             4854..5612
                     /gene="pdxJ"
                     /locus_tag="VD737_06880"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014605257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HXW10321.1"
                     /translation="MRLTHPIKLGINIDHVATLRQARRSPYPDPLYAALIAEQNGADS
                     ITLHLREDRRHIQDHDVRRMRATLQTRMNLEMAATDEMIGIACDVRPQDVCLVPEKRT
                     EVTTEGGLDVAGQRSRLRDCVAALRQTGIRVSMFIDPERDQLEASLDVGAPVVELHTG
                     AYAEATGEQRARELMRLVEAARHGSQIGLTVHAGHGLHYHNVQPVASVPEIVELNIGH
                     AIVARAVFDGLPAAVREMRRLMVEARRGLATGPG"
     gene            5621..6019
                     /gene="acpS"
                     /locus_tag="VD737_06885"
     CDS             5621..6019
                     /gene="acpS"
                     /locus_tag="VD737_06885"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HXW10322.1"
                     /translation="MIFGIGTDVVQLARVEEVYARHGEHFVRRLLLPEEEVAFRRYKR
                     PVRFLAMRFAAKEAIVKALGTGFAHGVWIRDVGITSNAWGRPEVIWSERGREVRDRLG
                     AGEGHVTLTDEAGLVVAVAVLMRRQEQAAP"
     gene            6016..6807
                     /locus_tag="VD737_06890"
     CDS             6016..6807
                     /locus_tag="VD737_06890"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HXW10323.1"
                     /translation="MTMIHNVFAFDIETVPDVEFGRRLHGLDGLTDKQVGYVMQTRQR
                     EQTGSEFLSLEQQRVVAISIAMRSRDGFRVWSLGEPDSTEEELVRRFFDGIERLSPTL
                     VSWNGSGFDLPVLHYRALRHRVQAHRYWEMGDEDQAFRWNNYLGRFHWRHTDLMDVLA
                     GYQGRGRVGLDKMAQLLGFPGKLGMSGDQVWDAHLAGRADDIRNYCETDVINTYLIYL
                     RFELMRGRLTPEEHDREVDLVRQALRESPRPHLQEYAQAWARCSC"
     gene            6821..8137
                     /gene="rlmD"
                     /locus_tag="VD737_06895"
     CDS             6821..8137
                     /gene="rlmD"
                     /locus_tag="VD737_06895"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:NF009639.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HXW10324.1"
                     /translation="MKRLQVAPEEADVVDLTHDAEGVVRIDGKTVFVADALPGERVRL
                     RRTHRHRKLDHAVTEAVLHASADRVAPRCPHFGTCGGCALQHLAPEAQLAFKQAQLVA
                     NLARLGGVQPARVLEPLAGPVWYYRRRARLGIKLVRAKGRVLVGFRERSSPFVTDLRE
                     CHVLVEPVGALITPLGALVEALSIADRVPQAEVAVSDEAVALVLRILAPPTPEDLQRL
                     ADFERAHPVRLYLQPGGPESVAPLHGDSADRPLSYRIADSGITIEFEPTDFIQVNGAL
                     NERMVARALAALDPGPDDSVLDLFCGLGNFSLPLARRAGAVVAVEGDAGLVARARRNA
                     ERNCVTNVEFHVANLFEDVVGLPWSRRRYDLALLDPPRAGAQQVLPVVAASGARRVVY
                     ISCHSGSLARDAGLLVREHGFELSAAGVMDMFPHTTHVEAMAIFDR"
BASE COUNT         1312 a         2837 c         2938 g         1269 t
ORIGIN      
        1 aggtcgacaa gctcgggcgg ttcacgccca agtcgatcgt gaccgcgtcg ctcgaaaccg
       61 gtgaagtggg cttcgtcgtc gccggcatca aggagatcga cggcgccccg gtgggtgaca
      121 cgatcacgct cgagtcacgt ccctgcaccg agccgctgcc cgggttcaag gcgatcaagc
      181 cgcgcgtgtt cgcgggcctg ttcccgacca gttccgacga ctacgaggcg ttccgcgacg
      241 ctctgcagaa gctgaagctg aacgactccg cgctgcacta cgagcccgag gtgtcgaccg
      301 cgctcggctt cggcttccgc tgcggtttcc tcggcctgct gcacatggac atcgtgcagg
      361 aacggctcga gcgcgaatac gaactcaacc tcgtgaccag cgcgccgacc gtggtctacg
      421 aggtcgtcac caccgacggc gacgtcgtga acgtcgacaa cccggccaag ctgccgccgc
      481 cggacaaggt cgcggaactg cgcgagccga tcatcgtcgc caacatcctg ctgccgccgg
      541 actacgtcgg gccggtcatg aagctgtgcc tcgagaagcg cggcgtgcag aaaaaattgc
      601 tgtacctcgg caaccaggtc tcgatgcagt gggagctgcc gctcgccgag gtcgtgctcg
      661 atttcttcga ccggctgaag tccgtgagcc gtggctacgc ctcgttcgac tacgagttcg
      721 atcgtttcca gggcgcgccg ctcgtcaagc tcgacatcct gatcaacgag gaaaaggtcg
      781 atgcgttgtc gatcatcagc caccgcgaca gcgcgtacca gcggggccgc gaactcgtcg
      841 acaagatgca ggagctgatc ccgcgccaga tgttcgaggt ggcgatccag gcggcgatcg
      901 gcgcgcacgt gatcgcgcgc gccaccgtca aggcactgcg caagaacgtg ctggccaagt
      961 gctacggggg cgacgtctcg cgcaagcgca agctcctcga gaagcagaag gaaggcaaaa
     1021 agcgcatgaa gcagctcgga tccgtcgaga tcccgcagga ggcgttcctc gccgtgctgc
     1081 aggtcggcag gaaggactga tgacggctgt gcgcacaatg tgacgcaaag cattgaataa
     1141 tcgaaataat tcctgaatac tgcgccgcgc tcgaggggtg ctcctttggt ctttgatttc
     1201 tcattcattc tcgtagtcgc cacgctcgtc accggcctga tctgggccgc cgacgcgtgg
     1261 gtgctgaagc cgcaacgcgt ggtcgctgcc aacggcgacg tggccgcgct gcgcgagccg
     1321 gtcgtggtgg agtacgcgcg ctcgttcttc ccgatcatcc tgatcgtgct ggtgatccgc
     1381 tcgttcctgt tcgagccgtt ccggatcccg tccgactcga tgatgccgac gctgctcgac
     1441 ggcgacttca tcttcgtcaa caagttcacc tacggcctgc ggctgccggt cgtgaacacc
     1501 gaagtggtgg cgctcggcga gccgcagcgc ggcgacgtcg tcgtcttccg cctgccgtcc
     1561 gatccctcga ccaactacat caagcgactg gtcggcctgc cgggcgatca cgtcgtcgtg
     1621 cgcgacaagc gcgtctacat caacggcgag ctgcagccgg tggagctcga cggcatgtcg
     1681 gatccttacg gcagcacgcc ggccgcgcag atcggcaagg aacgcttggg cgcggtggag
     1741 caccgcacgc tctacatcgc cgagcgcccg tcggtcgact tcaacgacgt cgtgccggcc
     1801 ggccactatt tcttcatggg ggacaaccgg gacaacagcc gcgacagccg tttcccggag
     1861 gtgggattcg tgccggccga ccacgtcgtg ggcaaggccg tgcggatctg gctcaactgg
     1921 aaccttcccg ctgcgccgct ctggggccgc atcggcgagg cgatccactg aactaacgtc
     1981 aacgaaacga gggagtacca gcgatgaagc atcgccagcg cggcgcaacg tttctcggca
     2041 tcgtcgtcat cctgctgatc ctcggcgcgg cgctctacgc gggcatccgg ctcgtgcccg
     2101 tgtacctcga gtacaccaag gtcgcgcgcg cgctcgagca ggtccgcgac gagaacgcgg
     2161 ccgtcgacac gaactcgcag cagatccgca acgcgctcga gcgccgctgg gacgtcgagg
     2221 acatcaagcg catcggctgg aaggagatcg acatcgccaa gaccaccgac gggttcgacg
     2281 tgtcggcgtc atacgaggcg gaggagccgt tcatcgcgaa cgtgtacctg ctcgtcaagt
     2341 tcgacaagac cgtcacgatc ccgcagtaat cgtgccgggg cgacttgcgc gacgccggtg
     2401 aatgggccgc ccgccggctc gggtatcgct tcggtgatcc cgcgctgctc gaggccgcgc
     2461 tgacgcatcg aagcgcgagc cgcacgaact acgagcggct cgagttcctc ggcgactccg
     2521 tgctcaattt cgtcgtcgcg ctgctcgcgt tccgcgcgtt ccccgaggcg ccggaaggcg
     2581 acctgagccg gttccgggcc gcgctcgtga gcgcgccgag cctcgcggcc gtggcgggtg
     2641 ccctggaact cggcgaacag ctgcggctcg gcagcggcga gctcaagtcc ggcggcttca
     2701 ggcgtgactc gatcctcgcc gacgcgctcg aggcggtcat cggcgcgatt tacctcgacg
     2761 gcgggctcga cgcggcgtcc gcgaccgtcg cgcgactgct cggcggcaag ctcgcggacc
     2821 tgccgtcggc cgagaccctc aaggacccga agacgcgcct gcaggaacaa ctgcagtcgc
     2881 gcgggctggc gctgccgcgg tacgcgctcg aggagatcgg cggcgaaccc catgagcagt
     2941 ggtttgtcgc aagctgtgag gcgcccggac tcggcttgcg attcgtcggc cggggatcga
     3001 gccggcgtcg ggccgagcag gaagcggccc agaaggtgct cgacgcgctc gggcacggag
     3061 aggagagcgc atgaaggacg agacggggca gggcccgcag gagaggccga tccgctgcgg
     3121 tttcgcggcg ctcgccgggc ggccgaacgt cggcaagtcg acgctgctga acacgctgct
     3181 cggtcgcaag gtcagcatcg tcagtcccaa gccgcagacg acgcggacgc gcatccacgg
     3241 cgtgctgaac cgcccggacc tgcagatcac gttcgtcgac ctgcccggca tccacgcgaa
     3301 gcagacgcgc gcgatgaacc ggtacatgaa ccgcacggcg atctcctcgc tgagcgacgt
     3361 cgacctgaac ctgttcgtcg tcgaggcgct gcgctggacc gacgaggacg agcgcgtgct
     3421 ggccgcgctg aagcaggcgg gactgccgat cgtgctggtg atcaacaagg tcgatcgcgc
     3481 gcagccccgc gagcgcctgc tgccgtacat cgaacagctc gcgcagaagg cggagttcgc
     3541 cgagatcgtg ccggtcgccg cgacgcgtcc ggcgtcgctc gaggcgctgc ccgggatcct
     3601 cgcgaagcac ctgccagagc agccacggat ttttccggaa gaccaggtca ccgattcgcc
     3661 cgatcacttc gtcgcggcag aactgatccg cgagaagctg atgctgcggc tgcaggagga
     3721 actgccctac ggcctcgtgg tcgagatcga gggcatgggc gactccgagg acgagcccgg
     3781 caagctgttc gtgcaggcgg tgatctgggt cgagcgcgcg gggcagaagg cgatcgtgat
     3841 cggcaagggc ggggaactgc tcaagacgat cggtcggcag gcacggctcg atctcaagga
     3901 gcacttcggg cgcgccgtgc acctcgagct ctgggtcaag gtcaaggaag gctggtccga
     3961 cgacgagaac gccatgcgca agttcgggta cgaatcgtga gcagcgcgcg gcgggtcagc
     4021 ctcgaacccg ccttcctgct gcaccactat ccctggcgcg acacgagccg gatcctggaa
     4081 ctgctgacgc gcacgcacgg ccgcgtctcg gtggtcgcgc gcggttcgcg ccagtcggcg
     4141 gcgacgacgg gcggctccct gcagtcgttc acggcctgcc tggtgtcgtg gagtgggcgc
     4201 ggcgagatgg gttacctgac cggcgtcgaa cgcctcctcc gcagctctcc cacatccgcg
     4261 ggagaggtcg cgcgcagcgc cggtgagggc tccggctctc ccgaaccctc acccgacctc
     4321 cggtcgacct ctcccggtgt accgggcgag gtgaagagcc tcgcgggcga ccgcctcatg
     4381 agcggcttct acgcgaacga gctgctgctc aggctgctgc agcgtcacga cccgcatccg
     4441 gcgctgttcg acgcctacgc gacgctgctg gagcgcctgc gggcggccga tgaggacgcc
     4501 gctcgagcac tgcgcatctt cgagaagcgg ctgctcgagg aactcggctg gggcctcaac
     4561 ctcgagcacg aggccgcgag cggtgcgccg gtcgaggcct cgcgcagcta ccgctacggg
     4621 ctcgagggcg gcgccgagcc gatcgatgga gtggcggcgg gatccctgat attttgcggc
     4681 gcgtcgctgc tgtcgctggc gggcgagacc ctcgacgacc cgcgcagcct cgccgacgcg
     4741 cgacggctgc tgcgggcggc gctcgaccgg ctgctcgacg gccgggcgct gcgcacgcgc
     4801 gacgtgctgc tcgaaatgcg cgcgcacgcc ggccatacgc agggagtctg acattgcggc
     4861 tcacgcaccc gatcaagctc ggcatcaaca tcgaccacgt ggcgacgctg cgtcaggcgc
     4921 gccgcagccc ctatccggac ccgctctacg ccgcgctcat cgccgagcag aacggcgccg
     4981 acagcatcac gctgcacctg cgcgaggatc gcaggcacat ccaggaccac gacgtgcgcc
     5041 gcatgcgcgc gacgctgcag acgcgcatga acctcgagat ggccgcgacc gacgagatga
     5101 tcggcatcgc ctgcgacgtg cgcccgcagg acgtctgcct ggtgccggag aagcgcaccg
     5161 aggtgaccac cgaggggggc ctcgacgtgg cggggcagcg gtcgcggctg cgcgactgcg
     5221 tcgcggcgct gcgccagacg ggcatccgcg tgtcgatgtt catcgatccc gagcgcgacc
     5281 agctcgaggc gtcgctcgac gtcggcgcgc ccgtggtcga actgcacacc ggggcctatg
     5341 ccgaggccac cggggagcag cgggcgcgcg agctgatgcg gctggtcgag gccgcgcggc
     5401 acgggtcgca gatcggcctc accgtgcacg cggggcacgg actgcactat cacaacgtgc
     5461 agcccgtcgc ttccgtgccc gagatcgtcg agctcaacat cggccacgcg atcgtggcaa
     5521 gagccgtctt cgacgggctg cccgcggccg tgcgcgagat gcgccggctc atggtcgagg
     5581 cccgccgcgg cctggccacg ggcccgggct gaccgcgcgc atgatcttcg gcatcggcac
     5641 cgacgtcgtg cagctcgcgc gcgtcgagga ggtctacgcc cggcacggcg agcacttcgt
     5701 gcggcggctg ctgctgccgg aggaggaggt cgcgttccgc cgctacaagc gtcccgtgcg
     5761 gtttctcgcg atgcgcttcg ccgcgaagga ggcgatcgtg aaggcgctcg gcaccggctt
     5821 cgcgcacggc gtctggatac gcgacgtcgg catcacgtcg aacgcgtggg gccggcctga
     5881 ggtgatctgg tcggagcggg gccgcgaagt gcgcgaccgg ctcggggccg gcgaggggca
     5941 cgtcacgctg accgacgaag cggggctcgt cgtcgccgtg gccgtgctga tgcgacggca
     6001 ggagcaggcg gcgccatgac gatgattcac aacgtgttcg cgttcgacat cgagaccgtg
     6061 ccggacgtcg agttcggccg gcggctgcac ggcctcgacg gactgaccga caagcaggtc
     6121 ggttacgtga tgcagacccg ccagcgcgag cagacgggct ccgagttcct gtcgctcgag
     6181 cagcagcgcg tggtagcgat ctcgatcgcg atgcgctccc gggacgggtt ccgcgtctgg
     6241 agcctcggcg agcccgactc gaccgaggag gaactcgtgc gccgcttctt cgacggcatc
     6301 gagcgactgt cgccgacgct cgtcagctgg aacggctcgg ggttcgacct gccggtgctg
     6361 cactaccgcg cgctgcggca ccgcgtgcag gcccaccgct actgggagat gggcgacgag
     6421 gaccaggcct ttcgctggaa caactacctc ggtcgcttcc actggcgcca caccgacctc
     6481 atggacgtgc tggccggcta ccagggccgc ggtcgcgtcg gcctcgacaa gatggcgcag
     6541 ctgctgggat tccccggcaa gctcggcatg tctggtgacc aggtctggga cgcgcacctg
     6601 gccggccggg ccgacgacat ccgcaactac tgcgaaaccg acgtcatcaa cacgtacctg
     6661 atctacctgc gtttcgagct gatgcgcggg cggctcacgc ccgaggagca cgaccgcgag
     6721 gtggacctcg tgcggcaggc gctgcgcgag tcgccgcggc cgcacctgca ggagtacgcg
     6781 caggcctggg cccggtgctc gtgctgacgg tggtcggggg atgaagcggc tgcaggtcgc
     6841 gccggaggaa gcggacgtcg tggacctgac gcacgacgcc gagggtgtcg tgcgcatcga
     6901 cggcaagacc gtgttcgtcg cggacgcgct gcccggcgag cgcgtgcgcc tgcgccgcac
     6961 gcatcgtcac cgcaagctcg accacgccgt caccgaggct gtgctgcacg cgagcgccga
     7021 tcgcgtggcg ccgcgctgcc cgcacttcgg cacctgtggc ggttgcgcgt tgcagcacct
     7081 ggcgcccgag gcgcaactcg cgttcaagca ggcgcagctc gtcgcgaacc tcgcgcggct
     7141 cggcggcgtg cagcccgccc gcgtgctcga accgctggcc gggccggtct ggtactaccg
     7201 gcgccgcgca cggctcggca tcaagctcgt gcgcgccaag ggcagggtgc tcgtcggctt
     7261 ccgcgagcgg tcgtcgccgt tcgtcacgga cctgcgagag tgccacgtgc tcgtcgagcc
     7321 ggtcggcgcg ctgatcacgc cgctcggggc attggtcgaa gcgctgtcaa tcgccgaccg
     7381 ggtgccgcag gccgaagtcg ccgtgtccga cgaagcggtc gccctggtgc tgcggatcct
     7441 ggcgccgccg acgccggagg acctgcagcg cctcgcggac ttcgaacgcg cgcatccggt
     7501 gcgcctgtac ctgcagcccg gaggacctga gtcggtcgcg ccactgcacg gcgattcggc
     7561 ggaccgaccg ctcagttacc ggatcgccga ctccggcatc acgatcgagt tcgagcccac
     7621 ggacttcatc caggtcaacg gtgcgctcaa cgaacggatg gtcgctcgcg cgctcgccgc
     7681 gctcgatccg gggcccgacg acagcgtgct cgacctgttc tgcgggctgg gcaatttctc
     7741 gctgccgctc gcgcgtcggg cgggggcggt cgtggcggtg gagggtgacg ccggactggt
     7801 tgcccgcgcc cggcgcaacg ccgagcgcaa ctgcgtcacg aacgtggagt ttcacgttgc
     7861 caacctgttc gaggatgtgg tggggttgcc ctggagcagg cgccgctacg acctggcgct
     7921 gctggacccg ccgcgcgcgg gcgcccagca ggtgctgccg gtcgtcgcgg cgagtggcgc
     7981 gcggcgcgtc gtgtatattt cctgccactc gggcagcctg gcgcgcgatg ccggcctgct
     8041 cgtgcgcgag cacggtttcg aactgtcggc ggcgggtgtc atggacatgt tcccgcacac
     8101 gacgcacgtc gaagccatgg cgatcttcga ccggtgaggc ccgcgatgcg gagtggtgcg
     8161 acgtcgaccg aggtgcctgg gtgacgctgg gtccgctgat ggtggacgtg gccggcaccg
     8221 acctcacggc cgaagaccgc cgggtcctgg cgcacccgct ggtcggcgcc gtgatcctgt
     8281 tcacccgcaa ctaccagggc tacgagcagc tcgagcggct cgtgcgcgag atccgcgcgg
     8341 tgcgcacgcc gccgct
//
LOCUS       DASPFR010000260         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_440844, whole genome shotgun sequence.
ACCESSION   DASPFR010000260 DASPFR010000000
VERSION     DASPFR010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440844"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2001)
                     /locus_tag="VD737_06900"
     CDS             complement(<1..>2001)
                     /locus_tag="VD737_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10325.1"
                     /translation="PLAGGPTTVAANIGGPDEAAQTIFFTFPPLPPIPVGVDPGNVVS
                     STPVNLEASGDELFFSAFGPNGRELYIVNTGGALAHFNLAAGTASSNPFGFTSVQTQV
                     GLRTYFVASGELWIDNGTAPGVQKIDLGGATGVSQLTTVGDKLYFRANNQLWVTAGLN
                     PLVATPFDQIIPPKVPLQVRVVAGEGDNQVTAGDLNAPTAFTADTMIGAEPVSVDLTE
                     AVRAALARGETRLTVRVENVSGDRDVMLSLAGPAREGLTGLQVNPSTPGLVADLLAGD
                     GTVIESGKAVIDLRAIESGRYFLRVYDPSGVANGDIPFKIEAIAPIQGYTHPVPDRDT
                     IHGEDGDDLIVGSQGLDRLWGDSGRDDFIGELVELKDFDRPAGEKQEPSLAEERSTIP
                     PEGPPVDAFIAIEDPGLRVAIAEALGLPITESYIDGQYVIHVPDRSLRTDLRLDESGV
                     WSQRIRASDLAQIVNLDASGLGISNLSGLQFAINVQTLDLASNSIGNFQLERLAPGTQ
                     SSGDARGFPRGMVDLENLLLDMNPLTDLGPLDPLLSLERLSIDGAQGETFMNQVPELF
                     WPVIGGGTRGLEFLSLDWIGARGFAGVPSNRAALIWIAEEGDYTFTLPLNVGFMRVNG
                     QIVYEAEPGAPTVNPIQLNRGWHIITVQGAPGLDVLFALDGGP"
BASE COUNT          288 a          687 c          709 g          317 t
ORIGIN      
        1 cggcccgccg tccagcgcga acagcacgtc caggccgggc gcgccctgaa cggtgatgat
       61 gtgccatccg cggttgagct ggatcgggtt gacggtcggc gcgcccggct cggcttcgta
      121 gacgatctgg ccgttgaccc gcatgaagcc gacattcagc ggcagcgtga aggtgtagtc
      181 accctcctcg gcgatccaga tcaacgccgc ccggttcgag gggacgccgg cgaagccgcg
      241 cgctccgatc cagtcgaggc tcaggaactc gaggccgcgc gtgccgccgc cgatcaccgg
      301 ccagaacagc tccggcacct ggttcatgaa cgtctcgccc tgcgcgccgt cgatcgacag
      361 ccgctcgagc gacagcagcg ggtcgagcgg cccgaggtcg gtgagcgggt tcatgtcgag
      421 cagcaggttc tcgaggtcca ccatgccgcg cgggaagccg cgcgcgtcgc cgctcgactg
      481 cgttccgggc gcgaggcgct cgagctggaa gttgccgatg ctgttgctcg cgagatcgag
      541 cgtctggacg ttgatcgcga actgcagccc cgagaggttc gagatgccca aaccggaggc
      601 gtcgaggttg acgatctgcg cgaggtcgct cgcgcggatg cgctggctcc acacgccgct
      661 ttcgtcgagc ctcaggtcgg tgcgcaggct gcggtccggg acgtgaatga cgtattgccc
      721 gtcgatgtag gactcggtga tcggcaggcc gagcgcctcg gcgatcgcga cgcgcaggcc
      781 cgggtcctcg atcgcgatga acgcgtcgac cggcgggcct tccggcggga tggtgctgcg
      841 ctcctcggcg agcgacggct cctgcttctc gcccgcgggc cggtcgaagt ccttcagctc
      901 caccagctcg ccgatgaagt cgtcgcgccc gctgtcgccc cacagccggt cgaggccctg
      961 gctgcccacg atgaggtcgt cgccgtcctc gccgtggatc gtatcgcggt cgggcaccgg
     1021 gtgggtgtag ccctggatcg gggcgatcgc ctcgatcttg aacgggatgt cgccgttcgc
     1081 tacgccgctc gggtcgtaga cgcgcaggaa gtagcggccc gattcgatcg cgcgcaggtc
     1141 gatcaccgcc ttgccggact cgatcaccgt gccgtcgccg gcgaggagat cggcgaccag
     1201 tcccggggtg ctcgggttca cctgcagccc ggtcaggcct tcgcgcgccg gcccggcgag
     1261 cgacagcatc acgtcgcggt cgcccgagac gttctcgacg cgcaccgtca gccgcgtctc
     1321 gccgcgagcg agcgcggcgc gcaccgcctc cgtgaggtcg accgacaccg gctccgcgcc
     1381 gatcatcgtg tcggcggtga aggcggtggg cgcgttcaag tcgccggcgg tgacctggtt
     1441 gtcgccttcg ccggccacca cccgcacctg gagcggcacc ttcggcggga tgatctggtc
     1501 gaacggcgtc gcgacgagcg ggttcaagcc cgccgtcacc caaagctggt tgttggcacg
     1561 gaagtacagc ttgtcgccga ccgtggtgag ctgcgagacg ccggtcgcgc cgcccaggtc
     1621 gatcttctga acccccgggg cggtgccgtt gtcgatccac agctctccgc tcgcgacgaa
     1681 gtaggtgcgc agtcccacct gcgtctgcac cgaagtgaag ccgaaggggt tcgagctcgc
     1741 cgtgccggca gcgaggttga agtgcgccag cgctccgccg gtgttgacga tgtagagctc
     1801 gcggccattc ggcccgaacg ccgagaagaa cagctcgtcg cccgaagcct cgagattgac
     1861 cggcgtcgag ctcaccacgt tgcccgggtc gactccgact ggaatcggcg ggagcggcgg
     1921 gaacgtgaag aagatggtct gcgccgcctc atccggcccg ccgatgttcg ccgcgaccgt
     1981 cgtcggtccg cccgcgagcg g
//
LOCUS       DASPFR010000261        11728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_928629, whole genome shotgun sequence.
ACCESSION   DASPFR010000261 DASPFR010000000
VERSION     DASPFR010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11728
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928629"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..1022
                     /gene="rplC"
                     /locus_tag="VD737_06905"
     CDS             324..1022
                     /gene="rplC"
                     /locus_tag="VD737_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012212557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HXW10326.1"
                     /translation="MTIGLIGRKAGMTRVFTDAGESIPVTVIEALPNRVTQVKGVESD
                     GYRAVQVTYGSKKPSRLSRAVAGHYAKAKVEPGESLVEFRLADGEGAELATGSEIKVD
                     MFAKGQVVDVTGTTIGKGFAGTMKRHNFAGGPASHGASVFHRAPGSIGQRQTPGRVFP
                     GKRMSGHMGDVRRTIENLQVVEIDAARNLLLIRGAVPGAPGGEVIVRPSLKAARLAKR
                     QKLAPKQPAAKTKK"
     gene            1041..1652
                     /gene="rplD"
                     /locus_tag="VD737_06910"
     CDS             1041..1652
                     /gene="rplD"
                     /locus_tag="VD737_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019519214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HXW10327.1"
                     /translation="MATQLKLQQGGDSIEVSEKAFGREFNEALVHQVVTAYMAGGRAG
                     TKRQKSKAEVRGGGIKPWKQKGGGRARAGSIRSPIWVGGGRAFAARPRDYTQKVNRKM
                     YRGAISALLSELARLERLTVVETLALDAPKTKLLATKLKELGLADVLIVVEAFDEKLE
                     LASRNLHGVTVLPVSAVDPVSLIRHERVLATVGAIRMLEEKLA"
     gene            1649..1951
                     /gene="rplW"
                     /locus_tag="VD737_06915"
     CDS             1649..1951
                     /gene="rplW"
                     /locus_tag="VD737_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HXW10328.1"
                     /translation="MSSKERLMQVLVAPHVSEKGARVAEQGNQVVFRVTRDATKPEIK
                     AAVELMFEVKVDAVQVVNVAGKAKRFGGRPGRRSDWKKAYVKLAQGQTIDFAGAEK"
     gene            1983..2807
                     /gene="rplB"
                     /locus_tag="VD737_06920"
     CDS             1983..2807
                     /gene="rplB"
                     /locus_tag="VD737_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HXW10329.1"
                     /translation="MALIKTKPTSPGRRFVVKADRSHLHKGEPVHALTASQKRTGARN
                     NMGRQTTRHKGGGHRQRYRVVDFLRNKDGVPGKVERLEYDPNRSAHLALVLYADGERR
                     YILAPKGLGAGDAVHSGADAPIKAGNALPLRNIPIGSLVHNVELKPGRGGQLGRSAGA
                     SVQLVAREGLYATIRLRSTEMRKVHVDCRATIGEVGNEENNLIVYGKAGAKRWRGIRP
                     TVRGVVMNPVDHPHGGGEGKSGQGNPHPVSPWGWKTKGKKTRTNKRTAGLIVRRRN"
     gene            2826..3104
                     /gene="rpsS"
                     /locus_tag="VD737_06925"
     CDS             2826..3104
                     /gene="rpsS"
                     /locus_tag="VD737_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HXW10330.1"
                     /translation="MPRSVKKGPFVDLHLAKKVQVASDKNDRRPIKTWSRRSMVLPDM
                     VGLTIAVHNGKQHVPVLVSENMVGHKLGEFAPTRTFRGHSGDKKASSE"
     gene            3108..3449
                     /gene="rplV"
                     /locus_tag="VD737_06930"
     CDS             3108..3449
                     /gene="rplV"
                     /locus_tag="VD737_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005319741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HXW10331.1"
                     /translation="MQVAAKLKYARISPQKCRLVADMVRGKPVGQALSTLRFTPKKGA
                     ELVRKVLESAIANAENNHNADIDALKVEVIDIGEAPRFKRFHARAKGRGNRIVKRNSH
                     ITILVGDGRKD"
     gene            3463..4092
                     /gene="rpsC"
                     /locus_tag="VD737_06935"
                     /pseudo
     CDS             3463..4092
                     /gene="rpsC"
                     /locus_tag="VD737_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185240.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            4197..4610
                     /gene="rplP"
                     /locus_tag="VD737_06940"
     CDS             4197..4610
                     /gene="rplP"
                     /locus_tag="VD737_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HXW10332.1"
                     /translation="MMQPKRTKYRKQFKGRNAGLAQRGSSVAFGDFGLKATSRARMTA
                     REIEAARRAMTRYVKRGGQVWIRVFPDVPVSKKPLEVRMGSGKGGVEYYVARVQPGKV
                     LFEMEGVTEDIAREAFKLAAAKLSVPTAFVKRQVR"
     gene            4610..4828
                     /gene="rpmC"
                     /locus_tag="VD737_06945"
     CDS             4610..4828
                     /gene="rpmC"
                     /locus_tag="VD737_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HXW10333.1"
                     /translation="MKARELRGKGSAELREELLKLRREQFNLRMAQASGQAAKPDQFG
                     KVRRNIARVKTVLGEQARAATAAKGEKW"
     gene            4825..5124
                     /gene="rpsQ"
                     /locus_tag="VD737_06950"
     CDS             4825..5124
                     /gene="rpsQ"
                     /locus_tag="VD737_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010318767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HXW10334.1"
                     /translation="MSEQQQAEQQSSADEARTQRTVTGTVVSAKMQKTVAVSVERLVK
                     HDRYGKYVRRTTKLLAHDEASECKEGDVVEIAECRRLSARKAWKVVRVVKQAAAV"
     gene            5153..5521
                     /gene="rplN"
                     /locus_tag="VD737_06955"
     CDS             5153..5521
                     /gene="rplN"
                     /locus_tag="VD737_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HXW10335.1"
                     /translation="MIQMRTMLAAADNSGAKRLMCIKVLGGSKRRYATVGDVIKVSVK
                     DAIPRGKVKKGEVYDAVVVRTAFGVRRPDGSLIRFDGNAAVLLTNKLEPIGTRIFGPV
                     TRELRTAQFMKIISLAPEVL"
     gene            5546..5860
                     /gene="rplX"
                     /locus_tag="VD737_06960"
     CDS             5546..5860
                     /gene="rplX"
                     /locus_tag="VD737_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HXW10336.1"
                     /translation="MNKIRKGDEVVVITGRDKGRRGTVIKVLGEKVLVENVNMVKRHT
                     RPNPQKGTQGGIVEKEAALHISNVQLWNPIAKKGDRVGVRTLGDGRRVRYFKSNDEVV
                     DA"
     gene            5876..6415
                     /gene="rplE"
                     /locus_tag="VD737_06965"
     CDS             5876..6415
                     /gene="rplE"
                     /locus_tag="VD737_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HXW10337.1"
                     /translation="MARLHTFYREKVVPELMQGLGVTNPMQVPKITKISVNMGVGEAV
                     ADKKAMDGAVSDLTALTGQKPVVTKSRKAIAAFKLRQGVPVGARVTLRGARMYEFLDR
                     LINVAMPRIRDFRGVSARSFDGRGNYTFGVKEQIIFPEIAYDQVDAIRGMDITITTTA
                     RDDREGRALLESFNFPFRK"
     gene            6432..6737
                     /gene="rpsN"
                     /locus_tag="VD737_06970"
     CDS             6432..6737
                     /gene="rpsN"
                     /locus_tag="VD737_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HXW10338.1"
                     /translation="MAKTSMIEREKRRGKLVKQHAAKRAKLKETIRSPKSTDAEREEA
                     QRKLQSLPRDSAASRVRNRCAITGRSRGVYRKFGLGRQKLREATMRGDIPGLGKASW"
     gene            6759..7154
                     /gene="rpsH"
                     /locus_tag="VD737_06975"
     CDS             6759..7154
                     /gene="rpsH"
                     /locus_tag="VD737_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HXW10339.1"
                     /translation="MSMTDPIADFLTRIRNGQLSGKPEIAAPSSRIKLALAKVLKDEG
                     YIEDFAVDGDSTKPTVKVRLKYYQGRPVIDRLERVSRPGLRVYKAKDELPKILGGMGV
                     AIISTSKGVMTDREARASGHGGEVLCIVS"
     gene            7184..7717
                     /gene="rplF"
                     /locus_tag="VD737_06980"
     CDS             7184..7717
                     /gene="rplF"
                     /locus_tag="VD737_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HXW10340.1"
                     /translation="MSRVAKRPVALPQGVTTTVDGSTVTVKGAKGALKLPLRPGLSLV
                     QEGQNVRVEMAERNKESLMHAGSLRSHLANSALGVSKGYEKKLELVGVGYRAAAQGKK
                     LNLTLGFSHPVEYDVPEGITIETPSQTEILVKGADKQQVGQVAAEIRAYRAPEPYKGK
                     GVRYSGERIELKETKKK"
     gene            7729..8079
                     /gene="rplR"
                     /locus_tag="VD737_06985"
     CDS             7729..8079
                     /gene="rplR"
                     /locus_tag="VD737_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HXW10341.1"
                     /translation="MEKKVSRLKRARRARAKIHELRTMRLSVHRTAQHIYAQVFDPQS
                     NVVASASTLQKDVAEGLKGTGNVEAAKAVGRAIAERAKAKGVTQVAFDRSGFKYHGRV
                     KALADAAREAGLEF"
     gene            8103..8609
                     /gene="rpsE"
                     /locus_tag="VD737_06990"
     CDS             8103..8609
                     /gene="rpsE"
                     /locus_tag="VD737_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005319713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HXW10342.1"
                     /translation="MARIEKVASQDEFIEKLVAVNRVSKTVKGGKQFGFTALTVVGDG
                     NGRVGFGYGKAREVPVAIQKAMQQARKNLVNVPLRNSTLHFAIVGKHGTSKVFMQPAA
                     DGTGVIAGGSMRAVFECAGVRNVLAKSYGSRNPINVVRATMNALTNMPAPERIAAKRG
                     KTVAEITG"
     gene            8615..8812
                     /gene="rpmD"
                     /locus_tag="VD737_06995"
     CDS             8615..8812
                     /gene="rpmD"
                     /locus_tag="VD737_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005350132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HXW10343.1"
                     /translation="MAKTQAQKTVKVTLVKSPFGELARHRATARGLGLRRMHQTVVVA
                     DTKEIRGMLNASRHLFRVEEA"
     gene            8820..9269
                     /gene="rplO"
                     /locus_tag="VD737_07000"
     CDS             8820..9269
                     /gene="rplO"
                     /locus_tag="VD737_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HXW10344.1"
                     /translation="MKLNSIGPAEGSKKPRTRVGRGASAGQGKTCGRGVKGQRARKGG
                     YHKVGFEGGQMPLQRRLPKIGFRSARARRTAEVRLSDLNKIEGGNVDLASLKAASLVP
                     ADTLHAKLVLSGEVKAAYTVSGIGVTKGARAAIEAAGGKVVEATAAA"
     gene            9376..10665
                     /gene="secY"
                     /locus_tag="VD737_07005"
     CDS             9376..10665
                     /gene="secY"
                     /locus_tag="VD737_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003304058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HXW10345.1"
                     /translation="MTELRRRFLFLIGGLIVYRIGTFIPVPGIDPVALARFFQDQAGT
                     ILSMFNMFSGGALQRLSIFALGVMPYISASIIVQMMAVVVPQWQQLRKEGEAGRRKLT
                     SYTRWGTVGLAGFQGAGAAVAFQNQGVVITPGFQFVFLATITLVAGTMFLMWLGEQIT
                     ERGLGNGISMIILAGIVAGLPAAIGGTLELVRNGEINPALALILVIMVAAVTYFVVFV
                     ERAQRRIPVNYAKRQQGRRVFAGTTTHLPFKLNMSGVIPPIFASSLLLFPATIASWFG
                     TNENFGWLQNIAASLGIGQPVHMMLYAGLIIFFCFFYTALVFNSRETSENLKKSGAFI
                     PGIRPGQQTAEYIDKVLTRLTVWGALYVTLVCLIPEILISQFQVPFYFGGTSLLIIVV
                     VIMDFMAQLQAHLMSHQYEGLMKKANLRGYGRPGQVR"
     gene            10695..10808
                     /gene="rpmJ"
                     /locus_tag="VD737_07010"
     CDS             10695..10808
                     /gene="rpmJ"
                     /locus_tag="VD737_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HXW10346.1"
                     /translation="MKVRPSVKRICKNCKVVRRRGVVYVICSDKRHKQRQG"
     gene            10888..11268
                     /gene="rpsM"
                     /locus_tag="VD737_07015"
     CDS             10888..11268
                     /gene="rpsM"
                     /locus_tag="VD737_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HXW10347.1"
                     /translation="MARIAGINIPMNKHVVIGLTSIYGVGRSQAKVACEAAGVKPETK
                     VKDLTEAEVAALRSQVAKLAVEGDLRREVAMNIKRLMDLGSYRGQRHRKGLPMRGQRT
                     RTNARTRKGPRKAAVKLNAPAGKA"
     gene            11307..11708
                     /gene="rpsK"
                     /locus_tag="VD737_07020"
     CDS             11307..11708
                     /gene="rpsK"
                     /locus_tag="VD737_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HXW10348.1"
                     /translation="MADDKKSGAAKPRKKVKRMVTDAVAHVHASFNNTIITITDRQGN
                     TLSWATSGGCGFRGSRKSTPFAAQVAAEKAGVAAQEYGVRTVDVKVRGPGPGRESAVR
                     ALNAAGLKVTSIEDVTPIPHNGCRPPKKRRV"
BASE COUNT         2157 a         3964 c         4030 g         1577 t
ORIGIN      
        1 ggacagtggt cagtggctag tgggccgccg gcggcgcagc cgcctcttta ccagccacca
       61 gccactgacc actagccact ttcctcttgc atagagctca cggcccgcta taatcgcggg
      121 ctctctcgcc gaggcctggc cggcattgcc ggtaccgctc gagcggcgcg atcgcccggc
      181 cgggcgggtc gctcctctca gcacggttcc cttcgacgag cttggtgccg cctttttcag
      241 gcgccgagaa cgacggtacg tatcgcagat tttcggtatc agcccgggcc aatcgcagca
      301 cgggcggtga acgaggacac aacatgacga tcggtctgat tggccgaaag gccggcatga
      361 cgcgcgtctt cacggacgcg ggcgaatcga tcccggtcac ggtgatcgaa gccctgccga
      421 accgcgtcac ccaggtgaag ggtgtcgagt ccgacggcta ccgcgccgtc caggtcacct
      481 acggcagcaa gaagccctcg cggctgagcc gcgccgtcgc cgggcattac gccaaggcca
      541 aggtcgagcc gggtgagtcg ctcgtcgagt tccgcctcgc cgacggcgag ggtgccgagc
      601 tcgcgacggg ttccgagatc aaggtcgaca tgttcgccaa gggacaggtc gtcgacgtga
      661 ccggcacgac catcggcaag ggcttcgccg gcaccatgaa gcgccacaac ttcgcgggtg
      721 gccccgcctc gcacggcgcc tccgtcttcc accgtgcccc gggctcgatc ggccagcgcc
      781 agacgccggg ccgcgtgttc cccggcaaac gcatgtccgg ccacatgggc gacgtgcgcc
      841 gcacgatcga gaacctgcag gtcgtcgaga tcgacgccgc gcgcaacctc ctgctgatcc
      901 gcggcgccgt tccgggcgcc ccgggcggcg aggtgatcgt gcgtccgtcg ctcaaggccg
      961 cgcggctcgc caagcgccag aagctggcgc cgaagcagcc ggccgcgaag accaagaagt
     1021 aacccgcccg ggaacccgac atggcaacgc aactgaagct ccagcagggc ggcgacagca
     1081 tcgaagtgtc cgagaaggcc ttcggccgcg agttcaacga ggcgctcgtc caccaggtcg
     1141 tcacggcgta catggccggc ggccgcgcgg gcaccaagcg ccagaagagc aaggccgagg
     1201 tccgcggcgg cggcatcaag ccgtggaagc agaaaggcgg cggtcgcgcc cgcgccggct
     1261 cgatccgcag cccgatctgg gtgggcggcg gccgtgcgtt cgccgcgcgc ccgcgcgact
     1321 acacgcagaa ggtcaaccgc aagatgtacc gcggcgcgat ctccgcgctg ctgtccgagc
     1381 tcgcgcggct cgagcgcctg acggtcgtcg agacgctcgc gctcgacgcg ccgaagacga
     1441 agctgctcgc gaccaagctc aaggagctcg gcctggccga cgtgctgatc gtggtcgagg
     1501 cgttcgacga gaagctcgag ctcgcctcgc gcaacctcca tggcgtcacg gtcctgccgg
     1561 tgtcggcggt cgatccggtc agcctgatcc gccacgagcg cgtgctcgcg acggtcggcg
     1621 cgatccgcat gctcgaggag aagctggcat gagcagcaaa gagcgtctga tgcaggtgct
     1681 ggtcgcgccg cacgtgtccg agaagggcgc gcgcgtggcc gagcagggca accaggtcgt
     1741 cttccgcgtg acgcgcgacg cgaccaagcc cgagatcaag gccgcggtcg agctgatgtt
     1801 cgaggtcaag gtcgacgcgg tgcaggtcgt gaacgtggcg ggcaaggcga agcgcttcgg
     1861 cggtcgcccg ggccgccgct ccgactggaa gaaggcctac gtgaagctcg cgcagggcca
     1921 gacgatcgat ttcgccggcg ccgagaagtg accggcgcgc acagcagacg gaaggcttaa
     1981 cgatggctct catcaagacc aaaccgacgt cgccgggccg ccgcttcgtc gtgaaggccg
     2041 accgctcgca cctgcacaag ggcgagccgg tgcacgcgct cacggcgagc cagaagcgca
     2101 cgggcgcccg caacaacatg ggccgccaga cgacgcgcca caagggcggc ggccaccgcc
     2161 agcgctaccg cgtggtcgac ttcctgcgca acaaggacgg cgtgccgggc aaggtcgagc
     2221 ggctcgagta cgacccgaac cgcagcgcgc acctcgcgct cgtgctgtac gccgacggcg
     2281 agcgtcgcta catcctcgcg ccgaagggcc tgggggccgg cgacgccgtg cactccggcg
     2341 ccgacgcgcc gatcaaggcc ggcaacgcgc tgccactgcg caacatcccg atcggctcgc
     2401 tcgtccacaa cgtcgagctg aagccgggcc gcggcggcca gctcggccgc agcgcggggg
     2461 cctccgtgca gctcgtcgcc cgcgaaggcc tctacgcgac gatccgcctg cgctcgaccg
     2521 agatgcgcaa ggtgcacgtc gactgccgcg cgacgatcgg cgaggtcggc aacgaagaga
     2581 acaacctgat cgtctacggc aaggccggtg ccaagcgctg gcgcggcatc cgcccgacgg
     2641 tccgcggcgt ggtcatgaac cccgtcgacc acccgcacgg cggcggcgag ggcaagtccg
     2701 gccagggcaa cccgcacccg gtgtcgccgt ggggctggaa gaccaagggc aagaagaccc
     2761 gcaccaacaa gcgcacggcc ggcctgatcg tccggcgccg caactgagcc tgagaggacc
     2821 tgccagtgcc ccgttcagtc aagaaaggcc cgttcgtcga cctccacctc gcgaagaagg
     2881 tgcaggttgc gtcggataag aacgaccgcc gcccgatcaa gacctggtcg cgccgctcga
     2941 tggtgctgcc cgacatggtc ggcctgacca tcgccgtcca caacggcaag cagcacgtgc
     3001 ccgtgctggt gtccgagaac atggtcggcc acaagctcgg cgagttcgcc ccgacccgca
     3061 cgttccgcgg ccactcgggc gacaagaagg cgtcctcgga gtaagccatg caagtcgcag
     3121 ccaagctcaa gtacgcgcgc atctctccgc agaagtgccg cctcgtcgcg gacatggtcc
     3181 gtggcaagcc ggtcggccag gccctatcga cgctgcgctt cacgccgaag aagggcgcgg
     3241 agctcgtccg caaggtgctc gagtcggcga tcgccaacgc cgagaacaac cacaacgcgg
     3301 acatcgacgc gctgaaggtc gaggtcatcg acatcggcga ggcgccgcgc ttcaagcggt
     3361 tccatgcccg cgccaagggc cgcggcaacc gcatcgtcaa gcgcaacagc cacatcacga
     3421 tcctcgtcgg cgacggccgc aaggactgag gtaggaagag ccatgggtca gaaggtcaat
     3481 ccggtcggca tccgtctcgg catcacgcgc gactggacgt cgaagtggta cgcgaacaag
     3541 cgcaacttcc cgagctacgt cctgcaggac tggcaggtcc gcgacttcct caagaagaag
     3601 ctggccgagg cgtcggtgag ccgcatccac atcgagcgcg ccgcccgcaa ggccaacatc
     3661 acgatccaca cggcccgccc gggcgtggtg atcggcaaga agggcgagga catcgagcgc
     3721 ctgcgcggcg aggtcgcccg gctgatgaag atgggcatga acgacgtgcg cctgaacatc
     3781 gccgagatcc gcaagcccga ggtcgacgcg cagctggtcg ccgagggcat cgcgcagcag
     3841 gtcgagcgcc gcgtgatgtt ccgccgcgcc atgaagcgcg ccgtcatgaa cacgatgcgc
     3901 agcggcgcgt tgggcgtgaa ggtgcgcctg tcgggccggt tgaacgggtc ggaaatcgcg
     3961 cgcaccgagt ggacgcgcga gggtcgcatc ccgctgcaca cgttccgtgc cgacatcgac
     4021 tacggcacgg ccgaggcacg cacgacctac ggcgtgatcg gcgtgaaggt gtggatcttc
     4081 aagggcgagg tgttcgacca gccggtgtcg cagagccagg gtaccgacgc cgaagcggcc
     4141 ccggccgcgg agccggcggc ctgagggccg cccgccccgg gacggagtaa acgaccatga
     4201 tgcaacccaa gcgaaccaag tatcgcaagc agttcaaggg ccgcaacgcg ggcctggccc
     4261 agcgcggctc gagcgtcgcg ttcggcgact tcgggctgaa ggcgaccagc cgcgcacgca
     4321 tgaccgcgcg cgagatcgag gcggcgcgcc gtgccatgac ccgctacgtg aagcgcggcg
     4381 gccaggtctg gatccgcgtc ttcccggacg tgcccgtcag caagaagccg ctcgaggtcc
     4441 gcatgggctc gggcaagggc ggcgtcgagt actacgtcgc gcgcgtccag cccggcaagg
     4501 tgctgttcga gatggaaggg gtgaccgagg acatcgcgcg cgaggcgttc aagctcgccg
     4561 cggccaagct gtcggtcccg acggcgttcg tgaagcggca ggtgcgctga tgaaggccag
     4621 agaactccgg ggcaagggct ccgcggagct gcgcgaggaa ctcctcaagc tgcgccgcga
     4681 gcagttcaac ctgcgcatgg cgcaggcgtc gggccaggca gccaagcctg accagttcgg
     4741 caaggtgcgc cgcaacatcg cgcgcgtgaa gacggtgctc ggcgagcagg cgcgcgccgc
     4801 gaccgcagcg aagggtgaga agtggtgagc gagcagcagc aggctgagca gcagtcgtcg
     4861 gccgacgagg cccgtaccca gcgcaccgtg accggcacgg tcgtgagcgc gaagatgcag
     4921 aagacggtcg cggtgtccgt cgagcggctc gtcaagcacg accgctacgg caagtacgtc
     4981 cgccgcacga ccaagctgct cgcccacgac gaggcctccg agtgcaagga aggcgacgtg
     5041 gtcgagatcg ccgagtgtcg ccgcctctcg gcccgcaagg cctggaaggt cgtgcgcgtg
     5101 gtcaagcagg ccgccgcggt ctgagccgca gccagcagca gaggacgaac ccatgatcca
     5161 gatgcgaacc atgctcgcgg ccgccgacaa cagcggcgcc aagcggctca tgtgcatcaa
     5221 ggtgctcggc ggctcgaagc gccgctacgc gacggtcggc gacgtgatca aggtgagcgt
     5281 caaggacgcg atcccgcgcg gcaaggtcaa gaagggcgag gtctacgacg cggtcgtcgt
     5341 gcgcaccgcc ttcggcgtgc ggcgcccgga cggctcgctg atccgcttcg acggcaacgc
     5401 cgcggtgctg ctcaccaaca agctcgagcc gatcggcacc cgcatcttcg gaccggtcac
     5461 ccgcgagctg cgcacggcgc agttcatgaa gatcatctcg ctcgcgccgg aagtgctctg
     5521 agcgcgtgag gaagaggaac gggacatgaa caagatccgc aagggcgacg aggtcgtcgt
     5581 catcaccggc cgcgacaagg gccggcgcgg caccgtcatc aaggtgctcg gcgagaaggt
     5641 cctcgtcgag aacgtgaaca tggtcaagcg ccacacgcgc cccaacccgc agaagggcac
     5701 gcagggcggc atcgtcgaga aggaggccgc gctccacatc tcgaacgtgc agctctggaa
     5761 cccgattgcg aagaagggcg accgcgtggg tgtccgcacg ctcggcgacg ggcgccgcgt
     5821 gcgctacttc aagtccaacg acgaagtcgt ggacgcctga tagaggtcgc ctgagatggc
     5881 aaggctgcat acgttttaca gggaaaaggt cgtgcccgag ctcatgcagg gcctcggcgt
     5941 gaccaacccc atgcaggtgc cgaagatcac caagatctcg gtgaacatgg gcgtgggcga
     6001 agccgtggcc gacaagaagg ccatggacgg cgccgtcagc gacctgactg cgctcaccgg
     6061 ccagaagccg gtggtgacga agtcgcgcaa ggcgatcgcc gcgttcaagc tgcgccaggg
     6121 cgtgccggtc ggcgcgcgcg tgacgctgcg cggcgcccgc atgtacgagt tcctcgaccg
     6181 cctgatcaac gtggcgatgc cgcgcatccg cgacttccgt ggcgtctcgg cccgctcctt
     6241 cgacgggcgc ggcaactaca cgttcggcgt caaggaacag atcatcttcc cggaaatcgc
     6301 ctacgaccag gtcgacgcga tccgcgggat ggacatcacg atcacgacga cggcacgcga
     6361 cgaccgggaa gggcgggcgc tgctcgagtc cttcaatttc ccgttccgca agtaggacag
     6421 gaacagaaca gatggcgaag accagcatga tcgagcgcga gaagcgccgc ggaaaactcg
     6481 tgaagcagca cgctgccaag cgcgccaagc tcaaggagac catccgcagc ccgaagtcga
     6541 cggacgccga gcgcgaagag gcgcagcgca agctgcagtc gctgccgcgc gattccgccg
     6601 cctcgcgggt acgcaaccgc tgcgcgatca ccggccgctc ccgcggcgtc taccgcaagt
     6661 tcgggctcgg gcggcagaag ctgcgcgagg cgacgatgcg cggggacatc ccgggcctcg
     6721 gcaaggccag ctggtaagcc gggtactgga gcaacagcat gagcatgacg gatcccatcg
     6781 cggacttcct gacccgcatt cgcaacggcc agctctcggg caagcccgag atcgccgcgc
     6841 cgtcgtcgcg gatcaagctg gcgctggcca aggtgctgaa ggacgagggc tacatcgagg
     6901 atttcgccgt cgacggcgac tcgaccaagc cgaccgtgaa ggtgcgcctg aagtactacc
     6961 agggccgccc ggtcatcgac cgcctcgaac gcgtgagccg cccaggactc cgcgtctaca
     7021 aggcgaagga cgagctgccg aagatcctcg gcggcatggg cgtggcgatc atctcgacct
     7081 ccaagggcgt gatgaccgac cgcgaggccc gtgcctcggg ccacggcggc gaagtgctct
     7141 gcatcgtgtc gtaaggcacg gccgcgaaca caggacgacc aacatgtcac gagtagcaaa
     7201 gaggccggtc gcgctgcccc agggcgtgac caccacggtc gacggcagca ccgtcaccgt
     7261 caagggcgcc aagggtgcgc tcaagctgcc gctgcgcccg ggcctgagcc tggtgcagga
     7321 aggccagaac gtgcgcgtcg agatggccga gcgcaacaag gagtcgctga tgcacgcggg
     7381 ctcgctgcgg tcgcacctcg cgaactcggc gctcggcgtg tcgaagggct acgagaagaa
     7441 gctcgagctc gtcggcgtcg gctaccgcgc cgccgcgcag ggcaagaagc tgaacctgac
     7501 gctcggcttc tcgcaccccg tcgaatacga cgtgccggaa ggcatcacga tcgagacgcc
     7561 gtcgcagacg gagatcctgg tgaagggcgc cgacaagcag caggtcggcc aggtcgccgc
     7621 ggagatccgc gcctatcgtg ccccggagcc ctacaagggc aagggcgttc gttattccgg
     7681 tgagcgcatc gagctcaagg aaacgaagaa gaagtaacgg gtgccgccat ggaaaagaaa
     7741 gtcagtcgtc tgaagcgtgc ccgccgggcc cgcgcgaaga tccacgagct gcgcacgatg
     7801 cgcctctcgg tgcaccgcac ggcgcagcac atctatgcgc aggtgttcga cccgcagtcg
     7861 aacgtggtcg cctcggcgtc gacgctgcag aaggacgtgg ccgagggcct caagggcacc
     7921 ggcaacgtcg aggccgcgaa ggccgtcggt cgcgcgatcg cggagcgtgc caaggccaag
     7981 ggcgtcacgc aggtcgcgtt cgaccgttcg ggattcaagt accacgggcg cgtcaaggcg
     8041 ctggccgacg ccgcgcgtga agccggcctc gagttctgag ccggaaggtt ccaggagcag
     8101 caatggctag gatcgaaaaa gtcgcgtcgc aggacgagtt catcgagaag ctcgtcgcgg
     8161 tcaaccgcgt gtccaagacc gtgaagggcg gcaagcagtt cggcttcacg gcgctgaccg
     8221 tcgtcggcga cggcaacggc cgcgtgggtt tcggctacgg caaggcgcgc gaagtgccgg
     8281 tcgccatcca gaaggcgatg cagcaggcgc gcaagaacct cgtcaacgtg ccgctgcgca
     8341 actcgacgct gcacttcgcg atcgtcggca agcacggcac ctcgaaggtg ttcatgcagc
     8401 cggccgccga cggcacgggc gtcatcgcgg gcggcagcat gcgcgcggtg ttcgagtgcg
     8461 ccggcgtgcg caacgtcctc gcgaagagct acggctcgcg caacccgatc aacgtcgtgc
     8521 gcgccacgat gaacgcgctc accaacatgc cggcgccgga gcgcatcgcc gccaagcgcg
     8581 gcaagaccgt cgccgagatc acgggttgac cgacatggcc aagacgcaag cgcagaagac
     8641 cgtcaaggtc accctggtga agagtccgtt cggcgagctc gcgcggcacc gcgcgacggc
     8701 gcgtggcctc gggctgcgcc gcatgcacca gacggtcgtc gtcgccgaca ccaaggaaat
     8761 ccgcggcatg ctgaacgcgt cgcggcacct gttccgcgtc gaagaggcgt gagcgaatca
     8821 tgaaactgaa cagcatcggc ccggcagagg gcagcaagaa gccgcgcacc cgcgtcggcc
     8881 gtggcgcgtc cgccggacag ggcaagacct gcggccgtgg cgtgaagggc cagcgcgcgc
     8941 gcaagggcgg ctatcacaag gtcggcttcg agggcggcca gatgccgctg cagcgccgcc
     9001 tgccgaagat cggcttccgc tcggcccgcg cccggcgcac ggccgaggtg cgcctgtccg
     9061 acctcaacaa gatcgagggc ggcaacgtcg acctcgcgtc gctcaaggcg gcgagcctgg
     9121 tgccggccga cacgctgcac gccaagctcg tgctgtcggg cgaggtcaag gcggcctaca
     9181 ccgtgagcgg catcggcgtc accaagggtg cgcgcgccgc gatcgaggcg gcgggcggca
     9241 aggtcgtcga ggcgacggcc gcggcctgag gccgcgatcc gcccccggca cacccgcagc
     9301 agcacagcac aggatccacg cgtggccaac ccgctctcca acaacccggt cgccgccttc
     9361 ggcgaggcga cccggttgac cgagctcagg aggaggttcc tcttcctgat cggcggcctg
     9421 atcgtctatc gcatcggcac gttcatcccc gtgccgggca tcgacccggt cgcgctcgcg
     9481 cgtttcttcc aggaccaggc cggcaccatc ctgagcatgt tcaacatgtt ctcgggcggg
     9541 gcgctgcagc gcctgtcgat cttcgcgctc ggcgtcatgc cgtacatctc ggcctcgatc
     9601 atcgtccaga tgatggccgt ggtcgtgccg cagtggcagc agctgcgcaa ggaaggcgag
     9661 gcgggccgcc gcaagctcac ctcgtacacg cgctggggca cggtcgggct cgccggcttc
     9721 cagggcgcgg gcgcggccgt ggctttccag aaccagggcg tcgtcatcac cccgggcttc
     9781 cagttcgtgt tcctcgcgac gatcacgctg gtggccggca ccatgttcct gatgtggctc
     9841 ggcgagcaga ttaccgagcg cggcctcggc aacggcatct cgatgatcat cctcgccggc
     9901 atcgtcgccg gcctgccggc cgcgatcggc ggcacgctcg agctcgtccg caacggcgag
     9961 atcaacccgg cgctcgcgct gatcctggtg atcatggtcg ccgcggtgac ctacttcgtg
    10021 gtgttcgtcg agcgcgccca gcgccgcatc ccggtcaact acgccaagcg ccagcagggg
    10081 cgtcgagtgt tcgcgggcac gacgacccac ctgccgttca agctcaacat gtccggcgtg
    10141 atcccgccga tcttcgcctc gagcctgctg ctgttcccgg cgacgatcgc cagctggttc
    10201 ggcaccaacg agaacttcgg ctggctgcag aacatcgcag cgagccttgg catcggccag
    10261 cccgtgcaca tgatgctgta cgcgggcctg atcatcttct tctgcttctt ctacaccgcg
    10321 ctggtcttca actcgcgcga gacgtcggag aacctcaaga aatcgggcgc gttcatcccg
    10381 ggcatccggc cgggccagca gaccgcggag tacatcgaca aggtgctgac gcgcctgacg
    10441 gtgtggggcg cgctgtacgt cacgctggtc tgcctgatcc cggagatcct gatttcccag
    10501 ttccaggtgc cgttctactt cggtggcacc tcgctcctga tcatcgtcgt ggtgatcatg
    10561 gacttcatgg cccagctgca ggcgcacctg atgtcgcacc agtacgaggg gctcatgaag
    10621 aaggccaacc tgcgcggcta cggccggccc ggccaggtgc gctgagacgc gccggctgca
    10681 acggagagtg atccatgaaa gttcgtccct cggtcaagcg gatctgcaag aactgcaagg
    10741 tggtgcggcg tcgcggcgtg gtctacgtga tctgcagcga caagcgccac aagcagcggc
    10801 agggctgatt ggcccgcctc ttgcattgag caaccctcgt ttttttgata ttattccgcg
    10861 cttttcgcgc cccggagtga gccaaaaatg gcccgtatag ccggtatcaa catcccgatg
    10921 aacaagcacg tcgtcatcgg gctgacctcg atctacggcg tcggtcgctc ccaggccaag
    10981 gtggcctgcg aggcggcggg cgtcaagccc gagaccaagg tgaaggacct gaccgaggcc
    11041 gaggtggccg cgctgcgttc gcaggtcgcc aagctggccg tcgagggtga cctccgccgc
    11101 gaagtcgcga tgaacatcaa gcggctcatg gacttgggct cgtaccgcgg ccagcgtcac
    11161 cgcaagggcc tgccgatgcg cgggcagcga acgcgcacca acgcgcgtac gcgcaagggc
    11221 ccgcgcaagg cggcggtcaa gctgaacgct ccggcgggca aggcgtaacg ccggcccggc
    11281 actgatcacg acaaggttgt acccgaatgg ctgacgacaa gaagtctggc gcagcgaagc
    11341 cccgcaagaa ggtcaagcgc atggtgaccg acgcggtggc gcacgtgcac gcgtcgttca
    11401 acaacacgat catcacgatc accgaccgcc agggcaacac gctctcgtgg gccacctcgg
    11461 gcggctgcgg cttccgcggc tcgcgcaaga gcacgccgtt cgcggcgcag gtcgccgccg
    11521 agaaggccgg tgtcgccgcg caggaatacg gcgtccgcac ggtggacgtg aaggtccgcg
    11581 gcccgggccc gggtcgcgag tcggccgtgc gcgcgctcaa cgccgccggc ctcaaggtca
    11641 ccagcatcga ggacgtgacg cccatcccgc acaacggatg ccgtccgccc aagaagcgcc
    11701 gcgtctgacg cgtcgctgcc gaggaatt
//
LOCUS       DASPFR010000262         4649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_929049, whole genome shotgun sequence.
ACCESSION   DASPFR010000262 DASPFR010000000
VERSION     DASPFR010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4649
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929049"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1296)
                     /locus_tag="VD737_07025"
     CDS             complement(100..1296)
                     /locus_tag="VD737_07025"
                     /inference="COORDINATES: protein motif:HMM:NF021500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HXW10349.1"
                     /translation="MRERARQGRTRRGTTTAAGVCCALAWLAASAEAVAAEPLFVLQD
                     ARGDDDGNGSLIYPNRDDLEPGDLDLVSLHAEQRSDGIWFVVEMGQAIRSPVGRVTEL
                     GQTPIERIARNGFYTFNVDVYVDTDRIAGSGQTGTVPGRAVTVDRQFAWEKALVLTPR
                     PDIARTMLQLYFDSEFESELRAKQGKVSKDELRDLQSQSERRVNDLFYFPTRVRVSGR
                     QVEFLVPQEFLGGVPSKSWGYTVVVTGADIEQTGRPGQLTPVKPTMMTMGVARGVRWS
                     QWGIRGDADEATPPVIDILAADHDVQKTALSDYDAVSGRLAAVPGVAPDGQAAMAGSG
                     QETTPEQLVRIEAATQGGTGTAGSTPTERRTVPARLRTLNELLADGLITQAEYNELRR
                     KILAEL"
     gene            complement(1366..3519)
                     /locus_tag="VD737_07030"
     CDS             complement(1366..3519)
                     /locus_tag="VD737_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10350.1"
                     /translation="MKFRNATLAAGIVAALGLAPQAGHAQAAQPGAIKWYGSIYAKFL
                     DGNRRTEQGLYSNSETTPGEGGGDQGQGIEFELLFNAQVSKAVEIGGRIHSRFNKNYW
                     ANYGGFAVPDNDTTNCGEDDPRCNQYVKLRGAWARITPGYDWMDSLTIGNSDWGMFDP
                     WTQGKSRYIDRDNIGGFLLQGSAMGKQLRWDLARVTLAQYQGSSYSTGTFGDDIYTND
                     ANYVGQLKYSPSPDWNATLIAMYARDKSLQDEPDPNPLNGIGVESAYENSVFGVRAQY
                     SGLGFVDLSGSFYASDYNAKNATDGNGSRCDQTWNGNCRFSPVPQTSTSDEAFIINAN
                     FNRLFIDGLSLSAQIFDIGADYVSITAARREADVLLTEGPEGTWQWGRPDYNFGNRAN
                     GNSMAGLGWGGWNGEVQQVVSGMADNDFTDFDEPVAQSVIGWKGITLVPKYAIGDWEF
                     AGEYSWIDFNNNWQACGGDDWNTDCGKYFKNEGTHAWGYGGDYRSPYSPYQDREMQIF
                     TVKANYTLDLGNGVDLAARYKYMSDEDKRVTRASHLAQAYNGYAGATINPEWIPNVGL
                     GGCLQCDDREVDYDTYGISAGYQLNPDLYVKLIYELHEVELIDGTVDVAPVGLGFEGS
                     NDFGYIEYVTGDTTKNRVALDFSYFLSGVEFGGTFDYLWGEYDPVFFTDSGGRRARLI
                     PGDGVTSIATPLGNIPISQVDYGQYRMKVFMKVSF"
     gene            complement(3550..4362)
                     /locus_tag="VD737_07035"
     CDS             complement(3550..4362)
                     /locus_tag="VD737_07035"
                     /inference="COORDINATES: protein motif:HMM:NF021500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HXW10351.1"
                     /translation="MKTSARVSICAMAVSFALAGAAGAAGVKFMDPTGDDKGPGTYVY
                     PTDAVYTPGSFDLVEFSVDGTDNLDFKVTLNDRLADPWGMGVGFATQMIFIFIDKDGE
                     AGKGHTESLPGLNVAFAPESAWDKVVILSPQPQSRAMAEVKAKAAGMAGDIIVPRRTG
                     GAGNTISAKVKLDELGGGDPATWGYQVVVQSNEGFPDKADLLTRKVNEFEGQHRFGGG
                     NDGECDPHVMDILAGKGEGKPEEAQAQYDMLKYECGADGSSTKKATLTVIRK"
     gene            complement(4416..>4649)
                     /locus_tag="VD737_07040"
     CDS             complement(4416..>4649)
                     /locus_tag="VD737_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXW10352.1"
                     /translation="LFSFMTGWNEFILAATFLEEETKYTAPVGLKFFVGGFTSQWGYF
                     AAGSIIVSVPVVVLFLFLQKYLVSGLTAGGVKG"
BASE COUNT          676 a         1595 c         1529 g          849 t
ORIGIN      
        1 ctgaacatgt tcagcccgcc acggtagtcg tggtacgcgt caccgaggcc gcgggctcgg
       61 gcgaggtcgt cgatcagcgc gtcgtcgtcg gccatcgcct cacagctccg cgaggatctt
      121 gcgccgcaac tcgttgtatt cggcctgggt gatcaaccca tcggccagca gctcgttgag
      181 cgtgcgcaac cgcgcgggca ccgtgcgccg ctcggtcggt gtgctgcccg cagtgccggt
      241 gccaccctgg gtcgcggcct cgatgcggac gagctgctcc ggcgtcgttt cctggccgct
      301 gccggccatg gcggcctggc cgtccggggc cacgcccggt acggccgcga ggcgaccgga
      361 tacggcgtcg tagtccgaaa gcgccgtctt ctggacgtcg tgatccgcgg cgaggatgtc
      421 gatgacgggc ggcgtggcct cgtcggcatc tccgcggatg ccccactgcg accagcgcac
      481 gccgcgcgcg acccccatcg tcatcatggt cggcttgacg ggcgtgagct gcccgggccg
      541 gcccgtctgc tcgatgtcgg cgccggtcac caccaccgtg tagccccacg acttcgacgg
      601 cacgccgccg aggaactcct gcggcacgag gaactcgacc tgccggccgc tcacgcgcac
      661 cctggtcggg aagtagaaca ggtcgttcac gcgccgctcg gactgcgact gcaggtcgcg
      721 cagctcgtcc ttcgacacct tgccctgctt cgcgcgcagc tcgctctcga attcggagtc
      781 gaagtacagc tgcagcatcg tgcgtgcgat gtccgggcgt ggagtcagca ccagcgcctt
      841 ctcccaggcg aactgccggt cgaccgtcac ggcgcgccca ggcacggtgc cggtctgccc
      901 ggagccggcg atgcggtccg tgtcgacgta gacgtccacg ttgaacgtgt agaaaccgtt
      961 gcgcgcgatg cgctcgatcg gcgtctggcc caattcggtc acacggccga ccgggctgcg
     1021 gatcgcctgg cccatctcga cgacgaacca gatgccgtcg ctgcgctgct cggcgtgcag
     1081 cgagacgagg tcgaggtctc ccggctcgag gtcgtcacgg ttgggataga tcagcgatcc
     1141 gttgccgtcg tcgtcgccgc gggcatcctg cagcacgaac agcggctcgg ccgcgacagc
     1201 ctctgcgctc gccgccagcc aggcgagcgc gcagcacacc ccggcggccg tcgtcgtgcc
     1261 tcgcctcgtc cgcccctgcc gtgctcgctc gcgcatctgc ctcaccgaaa aacaggggcg
     1321 gccacgcgga cgtgaccgcc cccgtcgctc gtcacgccgc aatcctcaga agctgacctt
     1381 catgaagacc ttcatgcggt actggccata gtccacctgc gagatcggga tgttcccgag
     1441 cggcgtcgcg atcgaggtga cgccgtcgcc cgggatcagc ctggcacggc ggccgccgga
     1501 gtccgtgaag aacaccgggt cgtactcgcc ccacaggtag tcgaacgtgc cgccgaactc
     1561 gacgcccgac aggaagtagc tgaagtcgag cgcgacgcgg ttcttggtcg tgtctccggt
     1621 cacgtactcg atgtagccga agtcgttgct gccttcgaac ccgagcccga ccggcgcgac
     1681 gtccaccgtg ccgtcgatca gctcgacctc gtgcagctcg tagatcagct tcacgtacag
     1741 gtcgggattg agctggtagc cggcgctgat cccgtaggtg tcgtagtcga cttcgcggtc
     1801 gtcgcactgc aggcagccgc cgagcccgac gttcgggatc cactcagggt tgatcgttgc
     1861 gccggcgtag ccgttgtagg cctgggccag gtgcgacgcg cgggtcacgc gcttgtcctc
     1921 gtccgacatg tacttgtagc gcgcggcgag gtcgacgccg ttgcccaggt cgagcgtgta
     1981 gttggccttg accgtgaaga tctgcatctc gcggtcctgg tacggcgagt acggcgagcg
     2041 gtagtcgccg ccgtagcccc acgcatgcgt gccttcgttc ttgaagtact tgccgcagtc
     2101 ggtgttccag tcgtcaccgc cgcaggcctg ccagttgttg ttgaagtcga tccagctgta
     2161 ctcgccggcg aactcccagt cgccgatcgc gtacttcggc acgagcgtga tgcccttcca
     2221 gccgatgacc gactgcgcga cgggctcgtc gaagtccgtg aagtcgttgt cggccatgcc
     2281 cgagaccacc tgctggacct cgccgttcca gccgccccag ccgaggccgg ccatcgagtt
     2341 gccattggcg cggttgccga agttgtaatc cggccggccc cactgccagg tgccctcggg
     2401 gccttccgtc agcagcacgt ccgcttcgcg tcgcgcggcc gtgatcgaga catagtcggc
     2461 gccgatgtcg aagatctgcg cgctcaggct caggccgtcg atgaacaggc ggttgaaatt
     2521 ggcattgatg atgaatgcct cgtcgctggt gctggtctgc ggcaccgggc tgaagcggca
     2581 gttgccgttc caggtctggt cgcagcgtga accgttgccg tcggtcgcgt tcttggcgtt
     2641 gtagtcggag gcgtagaagg aaccgctcag gtcgacgaag ccgagaccgc tgtactgcgc
     2701 gcgcacgccg aacaccgagt tctcgtacgc cgactcgacg ccgatgccgt tcagcgggtt
     2761 cgggtccggc tcgtcctgga gggacttgtc gcgcgcgtac atcgcgatca gcgtcgcgtt
     2821 ccagtcgggg ctcggcgagt acttgagctg gccgacgtag ttggcgtcgt tggtgtagat
     2881 gtcgtcgccg aacgttcccg tgctgtacga ggaaccctgg tattgcgcga gcgtcacgcg
     2941 cgccaggtcc cagcgcagct gcttgcccat cgccgagccc tgcagcagga agccgccgat
     3001 gttgtcgcgg tcgatgtagc gcgacttgcc ctgagtccac gggtcgaaca tgccccagtc
     3061 ggagttgccg atcgtgagcg agtccatcca gtcgtagccg ggcgtgatgc gcgcccacgc
     3121 gccgcgcagc ttcacgtact ggttgcagcg cggatcgtcc tcgccgcagt tcgtggtgtc
     3181 gttgtcgggc accgcgaagc cgccgtagtt ggcccagtag ttcttgttga accggctgtg
     3241 gatgcggccg ccgatctcga ccgccttcga gacctgggcg ttgaacagca gctcgaactc
     3301 gatgccctgc ccctggtcgc cgccgccctc gcccggcgtc gtctcggagt tcgagtacag
     3361 gccctgttcc gtgcgccggt tgccgtcgag gaacttggcg tagatgctcc cgtaccactt
     3421 gatcgcgccg ggctgggccg cctgcgcgtg cccggcctgg ggcgcgagcc ccagcgcggc
     3481 gacgataccg gccgcgagtg tcgcgttgcg aaatttcatg gtttccctcg ggtctgcgga
     3541 cccttgtcgt tacttgcgaa tgacggtcag cgtcgccttc ttggtcgagc tgccgtccgc
     3601 cccgcactcg tacttgagca tatcgtactg cgcctgggcc tcctcgggct tgccttcgcc
     3661 cttgcccgcg aggatgtcca tgacgtgcgg atcgcactcg ccgtcgtttc caccgccgaa
     3721 gcggtgctgg ccctcgaact cgttgacctt gcgggtcagc aggtcggcct tgtccggaaa
     3781 gccctcgttc gactgcacca cgacctggta accccaggtg gccggatcgc cgccgccgag
     3841 ttcgtcgagc ttgaccttcg cgctgatggt attgccggct ccgccggtac gccgcggcac
     3901 gatgatgtcg ccggccatac ccgccgcctt ggccttgacc tcggccatcg cgcgcgactg
     3961 cggctgcggg gacaggatca ccaccttgtc ccaggcgctc tcgggcgcaa aggccacgtt
     4021 gagccccggc aggctctcgg tgtgcccctt gccggcctca ccgtccttgt cgatgaagat
     4081 gaagatcatc tgggtcgcga agccgacccc catgccccac gggtccgcca accggtcgtt
     4141 gagcgtgacc ttgaagtcga ggttgtcggt gccgtcgacc gagaactcga ccaggtcgaa
     4201 cgaccccggg gtgtagacgg cgtcggtcgg gtacacgtag gtgcccgggc ccttgtcgtc
     4261 gccggtcggg tccatgaact tgacgcccgc cgcgccggcc gcgcccgcca acgcgaagct
     4321 caccgccatg gcacagatcg aaacgcgcgc gctggtcttc atcgtcgtac ctccgcttca
     4381 gcttgtcctt gaggccccgt caggggcgtg gttcgtcatc ccttcacgcc gccggcagtc
     4441 aggccggaga cgaggtattt ctgcaggaac aggaacagca ccacgaccgg gacggacacg
     4501 atgatcgacc cggccgcgaa gtaaccccac tgcgacgtga acccgccgac gaagaacttg
     4561 aggccgaccg gggccgtgta cttcgtctcc tcctcgagga acgtcgccgc gaggatgaac
     4621 tcgttccagc cggtcatgaa cgagaagag
//
LOCUS       DASPFR010000263         6759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1297054, whole genome shotgun sequence.
ACCESSION   DASPFR010000263 DASPFR010000000
VERSION     DASPFR010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297054"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="VD737_07045"
     CDS             complement(<1..1113)
                     /locus_tag="VD737_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXW10353.1"
                     /translation="MSLRALIVSDRPDYRQLLAHHVTLEWRDALPAEYEPATRGRLPA
                     GFTGVAYDVVLLDHEVQGGRGLEWIADFRGRTAFPPIVYFAPLVDPALLERARSTGAI
                     AVLPRTEFEHSELCGTLRRALAHRHNVLADTSRAALSQVPPDRFGSVRIRGYRCVRRI
                     AVGGSSSVYLANDTRSGDQRVLKIFRQVPDVVEGSTTFDRFLREYELVAHLNHPNIAR
                     IYDIGVADDHVYLSMEYFPGGDLRARMREKLGARAALGYVRQMAAALGALHEVGVLHR
                     DVKPGNLLLRQDGSVAFIDFGLARQLRLESDITGAGAIFGTPHYMSPEQGHGAPLDER
                     SDLYSLGVVLYEMLTGEKPYVAETPLAVIYLHANGPI"
     gene            complement(1110..2366)
                     /locus_tag="VD737_07050"
     CDS             complement(1110..2366)
                     /locus_tag="VD737_07050"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HXW10354.1"
                     /translation="MSPFRPSGELLIDCYRAALEAVDARRCVRDDLHRRRLAGEWHVV
                     AIGKAAGAMTQGAIDALGSRVAGGRVVVPPDHLPTDFTGAQYGIEVLTSSHPIPDRRS
                     LEAGESVAAYVSALPANAALLFLVSGGASSLVEWPRPGVTLDDLQALNRWALGAPVPI
                     NELNAFRRRLSRLKGGGLARLAGRRRSLALMISDVPGDDPRVIGSGLLHAAAPTTSEE
                     AERLPTELQAALDRAGADTVDATPRVPVGVVATLRMALHAAAAAARARGLQARVAPRR
                     LTGDAATVGRRLAGKLRALAPGTLQVWGGETTVALPSRPGRGGRNQHLALAAAIEFER
                     RPATPAALLAAGTDGIDGTSPDAGALVDAGTCLRGRDAGFDPHVSLASADSGSFLEAS
                     GDLLHTGATLTNVGDLVLGLGSLETQ"
     gene            complement(2537..4042)
                     /gene="purF"
                     /locus_tag="VD737_07055"
     CDS             complement(2537..4042)
                     /gene="purF"
                     /locus_tag="VD737_07055"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HXW10355.1"
                     /translation="MCGIVGIVGFSPVNQQIYDALTVLQHRGQDAAGIMTEYEGQLYL
                     RKDVGLVRDVFQQRHMEQLKGTIGMGHVRYPTAGCDGSDEAQPFYVNAPYGICLAHNG
                     NLTNAVELAEVLIREDRRHLNTSSDSEVLLNVLASELQRFGTPRASAADIFAALSATY
                     RRITGGYAVIVMILGHGILGFRDPHGIRPLVIGTRDTHKGREYMLASESVALDQLGFE
                     ILRDVGPGEAVYVDESGRLHTHQCAPPVLHTPCIFEYVYFARPDSIIDNISVYKARMR
                     MGELLAEKIRRTRPNHDIDVVIPIPDTSRTSAMQVAQILGVKYREGFIKNRYIGRTFI
                     MPEQAQRRKSVRSKLNAIDLEFRGKNVLLVDDSIVRGTTSAQIIEIAREAGANKVYFA
                     SAAPPVRYPNVYGIDMPAASELVAAGRTEDEIARLIGADWLIYQDLDDLVRAVRHHDS
                     GVVDFDTSCFSGEYVTGDITREYLDKLQSERSNQAKQTRSTGGLGLLKAVV"
     gene            complement(4067..4561)
                     /locus_tag="VD737_07060"
     CDS             complement(4067..4561)
                     /locus_tag="VD737_07060"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HXW10356.1"
                     /translation="MTPVDIILVAIVFISMLFGAIRGFLRESIALLGWLVGLWLAWRY
                     APELEPYLGGALAGTELQVWVARLILLFAAVIAAWLIGSLLGYIVQRSGLTLGLDRML
                     GAVFGIVRGAVIVGFAVMLADAAQLRGEEWWKSSKLIPVGEEMASILRGYVETGRRVI
                     EDAA"
     gene            complement(4620..5279)
                     /locus_tag="VD737_07065"
     CDS             complement(4620..5279)
                     /locus_tag="VD737_07065"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXW10357.1"
                     /translation="MDEQVKARLIGATVLVAIAVALVPELLSGPKHGTGPGAADPNKG
                     TRTVVIDLGGAVASGARVQTAEPAPTPAREPSRLTPVDVPGENVRRDDPAAEEAPTVE
                     PARPAATVAASEPMAPPVVAPASVAKPATTRGTKTTATSGTGGWAVQVGAFGSAESAR
                     KLVGELKKDGLSAYVAPLERNGKTLHRVRVGPAATRAEADKLAVRIKGRGLPASVVAP
                     D"
     gene            complement(5290..6582)
                     /gene="folC"
                     /locus_tag="VD737_07070"
     CDS             complement(5290..6582)
                     /gene="folC"
                     /locus_tag="VD737_07070"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF008101.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HXW10358.1"
                     /translation="MPAGPRFDRLDDWLAWQQQLHPSAIELGLERVGRVLARTGWRRP
                     DVPVVTVGGTNGKGSTVALLDALLRAAGHRVGTFTSPHLVDYRERIRMDGVKAGATAL
                     VAAFERIADALGPDSLTYFEFNTLAALLLFERADLDALVLEVGLGGRLDAVNVVDADV
                     AIVASVGLDHMDYLGTDEESIGREKAGIFRGGRPAIYGATDPPRSVRAEAARIGAELF
                     VRGERFDGRLRDHDDRWDFVIDGATLQHALPRPALPGRVQVGNAATALLALTQVADRL
                     PLDRAAIERGLQDVALPGRFQCISDARGFEWVLDVAHNPAAARALADNLRDGPVPGRT
                     LAVCGMLGDKDVAGVVATLRPLVDAWYAATPEGPRAIEARELAGRARDAGVEMRVGGD
                     VASAMQVAAADARAGDRVVVFGSFHTVGPALVALGVPL"
BASE COUNT         1030 a         2383 c         2360 g          986 t
ORIGIN      
        1 tatgggtccg ttggcgtgca ggtagatcac ggcgagcggc gtctcggcga cgtacggctt
       61 ctcgccggtc agcatctcgt acaacacgac gccgagcgag tagagatcgc tccgttcgtc
      121 gagcggcgcg ccgtggccct gctccgggct catgtagtgc ggcgtcccga agatcgcgcc
      181 tgcaccggtg atgtcgctct cgagccgcag ctgccgcgcg agcccgaagt cgatgaaggc
      241 cacgctgcca tcctgtcgca gcagcaggtt gccgggtttc acgtcccggt gcagcacgcc
      301 cacttcgtgc agtgcgccga gcgccgcggc catctgccgc acgtagccga gcgcggcacg
      361 ggccccgagc ttctctcgca tgcgcgcccg caggtcaccg cccgggaagt actccatcga
      421 caggtacacg tggtcgtccg cgacgccgat gtcgtagatc cgcgcgatgt tcgggtgatt
      481 caggtgcgcg acgagctcgt attcgcgcag gaaccggtcg aacgtcgtgc tgccttcgac
      541 gacgtcgggc acctggcgga agatcttgag cacgcgctga tcgccggaac gcgtgtcgtt
      601 ggcgaggtag acgctggagg agccgccgac cgcgatccga cgcacgcacc ggtagccgcg
      661 gatgcgcacc gagccgaacc ggtccggcgg cacctgcgac agcgccgcgc gcgaagtgtc
      721 ggcgagcacg ttgtgccggt gcgccagcgc gcgacgcagc gttccgcaca gttccgaatg
      781 ctcgaactcc gtgcgcggca gcacggcgat ggcgccggtc gaacgcgcgc gttcgaggag
      841 cgccgggtcc acgagcggcg cgaaatacac gatcggcgga aacgccgtcc ggccccggaa
      901 atcggcgatc cactcgagac cgcgcccgcc ctgcacttcg tgatcgagca gcacgacgtc
      961 gtacgccacc ccggtgaatc cggcaggcag ccgaccgcgc gtcgcgggct cgtactcggc
     1021 cggcaacgcg tcgcgccact ccagggtgac gtgatgcgcg agcaactgcc gataatccgg
     1081 ccggtcgctc acgatcaatg cccgcaggct cattgcgtct cgaggctccc caggcccaat
     1141 acgaggtcgc cgacgttggt cagcgtcgcg ccagtatgca gcaggtcgcc tgacgcctcg
     1201 agaaaactac ccgaatcggc gctcgcgagc gatacgtggg gatcgaagcc cgcgtcgcgt
     1261 ccgcgcaggc acgtgccggc gtcgaccagc gcaccggcgt ccggcgaagt gccgtcgatg
     1321 ccgtcggtgc ccgcggcgag cagcgccgca ggtgtcgccg ggcgtcgctc gaactcgatc
     1381 gccgcggcaa gcgccaggtg ctggttgcgg ccgccacgac cgggtcgcga cggcagcgcc
     1441 acggtcgttt cgccgcccca gacctgcagc gtgcccggcg ccagggcgcg gagctttcct
     1501 gcgaggcgac gaccgaccgt ggccgcgtcg cccgtcagcc gacgcggcgc gacgcgcgct
     1561 tgcaggcccc gtgcgcgggc ggccgcggcg gccgcgtgca acgccatgcg cagcgtcgcg
     1621 acgacgccca cggggacgcg cggagtggcg tcgacggtgt cggcgccggc gcggtcgagc
     1681 gccgcttgca gttccgtggg caggcgctcg gcttcctcgc tggtggtggg cgctgccgcg
     1741 tgcaggagcc ctgagccgat cacccgcggg tcgtcgcccg gcacatccga gatcatcagc
     1801 gcgagcgagc gacgccgacc cgccaggcgc gccagcccgc cgcccttgag ccgcgagaga
     1861 cgccgtcgga acgcattgag ttcattgatc ggcacgggcg caccgagcgc ccagcgattg
     1921 agcgcctgca ggtcgtcgag cgtcacgccg ggtcgcggcc actcgaccag gctcgaggcg
     1981 ccgcccgaga cgaggaacag caacgctgcg ttcgcgggca gcgcgctcac gtaggctgcc
     2041 accgactcgc cggcctcgag gctgcgccgg tccgggatcg ggtgcgagga cgtcaggact
     2101 tcgatgccgt attgcgcgcc ggtgaaatcc gtcggcaggt gatccggcgg cacgaccacg
     2161 cgtccgccgg ccacgcgcga gccgagcgca tcgatggccc cctgcgtcat cgccccggcc
     2221 gccttgccga tcgcgacgac gtgccactcg cccgcgagcc gccgtcggtg aaggtcgtca
     2281 cgcacgcaac ggcgcgcgtc gaccgcctcg agcgcggcgc gatagcagtc gatcagcagc
     2341 tccccggacg gcctgaatgg ggacactcct gaatttcgcc tgaatgggga cactcctgtt
     2401 tttcgtgctg gagattcagg ggtcgaaaat ggggacactc cggatttccg tcgccgtgcg
     2461 ccgaatattc ccggggattt cagaaaatca ggagtgtccc caatttcctg gaaattcgga
     2521 gtgtccccat ttaaggctag acgacggctt tcaggaggcc gaggccgccg gtgctgcgcg
     2581 tctgcttggc ctggttcgag cgctcggact gcagcttgtc gaggtattcg cgggtgatgt
     2641 cgccggtgac gtactcgccc gagaagcacg aggtgtcgaa gtcgacgacg ccggaatcgt
     2701 ggtgccggac ggcgcgcacg aggtcgtcga ggtcctggta gatcagccag tccgcaccga
     2761 tcagcctggc gatctcgtcc tcggtgcgcc ccgcggccac gagttcggaa gccgccggca
     2821 tgtcgatgcc gtagacgttc gggtatcgca cgggcggcgc ggccgaggcg aaatacacct
     2881 tgttggcccc cgcctcccgc gcgatctcga tgatctgcgc cgaggtggtg ccgcggacga
     2941 tcgagtcgtc caccagcagc acgttcttgc cgcggaactc gaggtcgatc gcgttcagct
     3001 tgctgcgcac ggatttccgg cgctgcgcct gctccggcat gatgaacgta cggccgatgt
     3061 agcggttctt gatgaacccc tcgcggtact tgacgccgag gatctgcgcg acctgcatcg
     3121 cgctcgtgcg gctcgtgtcc gggatcggga tcaccacgtc gatgtcgtgg ttgggtcgcg
     3181 tgcggcggat cttctcggcc agcagctcac ccatgcgcat gcgggccttg tagaccgaga
     3241 tgttgtcgat gatcgagtcg ggacgcgcga agtacacgta ctcgaagatg cacggggtgt
     3301 gcagcacggg cggggcgcac tggtgcgtgt gcaggcggcc cgattcgtcg acgtagacgg
     3361 cctcgcccgg accgacgtcg cgcaggatct cgaacccgag ctggtcgagc gcgacgctct
     3421 ccgaggcgag catgtattcg cggcccttgt gcgtgtcgcg cgtgccgatc accagcggcc
     3481 ggatgccgtg cggatcgcgg aagcccagga tgccgtgacc gaggatcatc acgatgaccg
     3541 cgtacccgcc ggtgatgcgg cgataggtcg cgctcagcgc ggcaaagatg tctgcggcgg
     3601 aggcccgcgg cgtgccgaag cgctgcagct cgctcgcgag cacgttgagc agcacctccg
     3661 aatcggacga ggtgttgagg tgacgccggt cctcgcggat caggacctcg gcgagctcga
     3721 ccgcgttcgt gaggttgccg ttgtgcgcga ggcagatgcc atacggcgca ttgacgtaga
     3781 acggctgcgc ttcgtcggag ccgtcgcagc ccgccgtcgg gtatcgcacg tggcccatgc
     3841 cgatcgtgcc cttgagctgc tccatgtggc gctgctggaa cacgtcgcgg accagcccga
     3901 cgtccttgcg caggtacagc tggccttcgt actcggtcat gatgcccgcg gcgtcctggc
     3961 cgcgatgctg caacacggtc agcgcgtcgt agatctgctg gttgacgggg ctgaacccca
     4021 cgatgccgac gatgccgcac atggcctgaa ggtcctcaga ggttgcctag gccgcgtcct
     4081 cgatgacgcg ccgcccggtc tcgacatagc ctcgcagaat cgacgccatc tcctcgccga
     4141 ccggaatcag cttggagctt ttccaccatt cctcgccgcg cagctgggcc gcgtcggcca
     4201 gcatcacggc gaacccgacg atgaccgcgc cgcggacgat cccgaacacc gcgccgagca
     4261 ttcgatcgag cccgagcgtc aggcctgaac gctgcacgat gtatccgagc aggctcccga
     4321 tcagccaggc cgcgatgacc gctgcgaaga gcagtatcag acgcgcgacc cacacctgaa
     4381 gttcggtgcc ggcgagcgca ccgcccagat aaggctcgag ctcgggggca tagcgccagg
     4441 cgagccacag gccgacgagc cagccgagca gcgcgatgga ctcccgcagg aacccccgga
     4501 tcgcgccgaa cagcatggag atgaagacga tcgcgaccag gatgatgtcg acgggggtca
     4561 tgcgagggat gggcgtcccg tgtcgagaag cgagccgtaa ttctagcaga gccgcccgct
     4621 cagtcgggcg ccacgaccga ggcgggaagc ccgcgcccct tgatccgcac ggcgagcttg
     4681 tcggcctccg ctcgcgtggc cgccggtccc acgcgcacgc ggtgcagcgt cttgccgttg
     4741 cgctcgagcg gcgcgacata agccgacagc ccgtccttct tgagctcgcc gacgagcttg
     4801 cgggcgctct cggccgaacc gaacgcgccg acctgcacgg cccatccgcc cgtgccagac
     4861 gtggcggtgg tcttcgtgcc gcgggtcgta gccggcttcg caaccgaggc tggcgcgacg
     4921 accggcggcg ccatcggctc gctcgcggcg accgtcgcgg cgggcctcgc cggctcgacg
     4981 gtcggcgcct cttcagcggc cggatcgtcc ctgcgcacgt tctcgcccgg cacgtcgacg
     5041 ggtgtcagcc gcgacggttc ccgtgcgggg gtcggcgccg gctcggccgt ctgcacgcgt
     5101 gccccgctcg cgacggcgcc tcccaggtcg atgacgaccg tgcgcgtgcc cttgttcggg
     5161 tccgcggcac ccggccccgt gccgtgcttt ggcccggaca gcagttccgg cacgagcgcg
     5221 accgcaatcg cgaccagcac cgtcgccccg atcagccgcg ccttgacctg ttcgtccatc
     5281 ggccgcggat tataggggaa cgcccagcgc aacgagcgct ggaccgaccg tgtggaacga
     5341 gccgaagacg acgacgcgat cgccggcacg cgcgtcggcg gcggccacct gcatcgccga
     5401 ggccacgtca ccgcccaccc gcatctcgac gcccgcgtct cgcgcgcgcc cagccaattc
     5461 acgcgcctcg atcgcgcgcg ggccctcggg cgtcgccgcg taccacgcgt cgaccaacgg
     5521 gcgcagcgtc gcgacgacac cggccacgtc cttgtcgccg agcatgccgc acacggcgag
     5581 cgtgcggccg ggtacgggcc cgtcgcgcag gttgtcggcg agcgcgcggg cggccgcagg
     5641 gttgtgcgcc acgtccagca cccactcgaa gccgcgcgcg tcgctgatgc actggaagcg
     5701 ccccggcagc gccacgtcct gcaggccgcg ctcgatcgcg gcgcgatcca gcggcaggcg
     5761 atcggcgacc tgcgtcaacg cgagcagggc cgttgctgcg ttgccgacct gcacgcgacc
     5821 cggcaacgcc ggcctgggca acgcgtgctg cagggtcgcg ccgtcgatca cgaaatccca
     5881 gcggtcgtcg tggtcgcgca ggcggccgtc gaaccgctca ccacgaacga acagctcagc
     5941 cccgatgcgg gccgcctcgg cacgcaccga acggggcgga tcggtcgcgc cgtagatggc
     6001 gggccgcccg ccacgaaaga tgcccgcctt ctcgcgaccg atcgattcct cgtccgtgcc
     6061 caggtagtcc atgtggtcga gtccgacgga agcgacgatc gcgacgtcag cgtcgacgac
     6121 gttcaccgcg tcgagccttc cgcccaagcc cacctcgagg accagcgcgt cgaggtccgc
     6181 gcgctcgaac agcagcagcg ccgccagggt gttgaactcg aagtacgtga gcgagtcggg
     6241 acccagggcg tcggcgatgc gctcgaacgc cgccaccagg gccgtggcac cggccttgac
     6301 gccgtccatg cggatccgct cgcggtagtc gacgaggtgc ggtgacgtga acgtgccgac
     6361 gcgatgaccc gcggcccgca gcagggcgtc gagcagcgcc accgtcgagc ccttgccatt
     6421 ggtgccgccg acggtgacga ccggtacgtc cggccggcgc caccccgtgc gcgcgagcac
     6481 gcggcccacg cgttcgagac cgagctcgat cgcggaagga tgcagctgct gctgccaggc
     6541 cagccagtcg tcgagccggt cgaaccgcgg gccggcgggc atcgcgtgcg cgcgaccgct
     6601 cagggcgaga cgggttgtat gaacgtcccg gtgtgctcgt ccggccgcgc aagcggtgga
     6661 cgtcgcagca tcacggccag cagtgccgcg atgcggtcgc gcaggtcgcg ccggtcgatg
     6721 atcaggtcga tcgcgccgtg ctcgagcagg aactcgctg
//
LOCUS       DASPFR010000264         5878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1783982, whole genome shotgun sequence.
ACCESSION   DASPFR010000264 DASPFR010000000
VERSION     DASPFR010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783982"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5859)
                     /locus_tag="VD737_07075"
     CDS             complement(<1..5859)
                     /locus_tag="VD737_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10359.1"
                     /translation="MTATGSDALELSANDASIFAITIRSGGADLGALGLRNGIDRALL
                     GVTGPAGDALRLYPGERLEQATGTEETPGLEEEPDLWRFELANPTAPGFVPSPLPGVD
                     LANDFTPNLRDAFVLEGAQEEERLAGSVSMADFNGDSFNDLLVYGDKASYVLLGPVEL
                     DSVESIAQAADIVIAADVGRPASRMGDLTGDGLDDLVFLKQTVGDDFELIIIAGGNGA
                     GIELPRVIDYAWVDATNDVLNQERVRIRSATGLPEGFADPSASLAVLNWNDDGHADVA
                     LVRSSAPSFNVQGYMLSGDALWNEVFDALDPDGTTGLGIIVSDTTDREMAAREILGVA
                     EGEPLAAFTELSTHVRAVAAGDITGDGLDDLLLADSGYVDFTGTEGFSNIGKAYVLTG
                     RVGAPTLLWLDAPQEAIDFLGGVKSELVVQDFSLGGSVSALGDLNFDGYDDFAVGSLA
                     EGQKAFPGDTVQDAGLLVYFGQAGFDAEPLSARDADIRIVRAAGDDVLPGSLFHGALH
                     ATGGDFDGDGHADLAVGETARLVTTGQQIIDQDDSGALYVFSRVADGSTVKVLTEADA
                     RVRGEFEFDGFGALPATPAFDLDLDGLDDLLVGAATANVTTTEVIPEGGKIYVIYGSS
                     SRAALPADAIELGNRSFTGAGFFLVGKNLGEVEVFQDAPGEDEPKFILDNGNERWYTF
                     QTLGDGMPGNVIRILSGLGAIDGFVDPIAPTTSTLEAGTPSTDGNRLIQRNTVDGAVG
                     SMFVAGAFQESGRLTQWSVFSGNFVDGVGAGDDRYLTPLIFKQTAGGFQITGIGKAER
                     IAPNSTLTFDFELESGSDSVGPGYRLGWYDGSAAGENAGAVGFNTFAFSGDEFGAVGW
                     FGPNQGAAQNLELNRTLNPIQTFTRYYAINASVTSGAVLEFDLGRFLGWAGNPDAVGS
                     AQLVLHAPQTTAPIQAPVNIGAILPVGNLVFFSASTPGEGQELWVTEGTEETTRFVAD
                     LNPGADGSFPGNFVNVGGKLYFTANTGVAGTQLWKSDGTTDGTQKVADIPGFAANLTA
                     LSGSAVLTAATAGPSDGIPAVDYAFTLEILRANGTLESEAVTLPRAATAENGSFSMLL
                     LDVQNAVAAALPGEVSVSTPDGERFVFSALDEDIVRIRVLGGEALGFGANQASEQQVS
                     LTGAPVTAPNVGAVSFTLDVEVIGGDVISVEVPRTAEVTADNTTAEHLAADLNAILPA
                     ALLSNGFGSGAVTVSADSSGALVLTVGDANIASITVRAGTGTAAGLASLGLTEGQTST
                     RTVALTATLAGPTDGQVPADLTLTVTVVLSTGETEELAITLEGGDTALNGSLDDLAGQ
                     LEEAINLELGLGEGVVSVDASPSFILTIVSSDASITSIAIDGGELLGFTAGQTSARSG
                     DRLYFRAAAASQGAELWVLNVDETDAAPDVPASFDVLSGPNGSNPFDLTKVGNTLYFA
                     AFNGTTNALYRVDLGVRVESGTPVQVPGPFTNVAGLINANGTLAFVALPNGLSDRQVF
                     TYSGTAFTQLTNVTPTDVTDFSPSELTYFNNRIYFAARDHADGEFTSPAGPLFVGREL
                     WSVPLAGGPTTVAANIGGPDEAAQTIFFTFPPFPPIPIGTDPGNVVSSNPFNLEASGD
                     RLFFSAQGPNGRELYMHSGVVLTPFNLAPGTASSFPSSFTPVDTQAGLRTYFVANGEL
                     YISDGTFGGTKKMLNVATGVGQLTAVGDRLYFRAGNQLWVTGSNPEAATPFEEIIPPK
                     VPLQVRVVAGEGDNQVTTDDLNARTVFTADTEIGAEAVSVDLTQAVRDALARGETRLT
                     VRVENVSGDRDVILSLAGPARDGQTGLQVNPSSPGLVADLLAGDGTVIETGKAVIDLR
                     AIESGRYYLRVYDPSGQANGDIPFKIEAIAPIQGYTHPVPDRDTIHGEDGDDLIVGSQ
                     GLDRLWGDSGRDDFIGELVELKDFDRPAGEQQAPSLPEERSTIPPEGPPVDGF"
BASE COUNT          844 a         1993 c         2129 g          912 t
ORIGIN      
        1 gaacccgtcg accggggggc cttcgggcgg gatggtgctg cgctcctcgg gcagcgacgg
       61 ggcctgctgc tcgccggccg gccggtcgaa gtccttgagc tcgaccagct cgccgatgaa
      121 gtcgtcgcgg ccgctgtcgc cccacaggcg gtccaaaccc tggctaccga cgatgaggtc
      181 gtcgccgtcc tcgccgtgga tggtgtcgcg gtcgggcacc gggtgcgtgt agccctggat
      241 cggcgcgatc gcctcgatct tgaacgggat gtcgccgttc gcctggccgc tcggatcgta
      301 gacgcgcagg tagtagcggc ccgactcgat ggcgcgcagg tcgatcaccg ccttgccggt
      361 ctcgatcacc gtgccgtcgc ccgccagcag gtcggcgacc aggccggggc tgctcggatt
      421 cacctgcagc ccggtctgcc cgtcgcgcgc cgggccggcg agcgacagga tcacgtcgcg
      481 gtcgcccgag acgttctcga cccgcaccgt gaggcgcgtc tcgccgcgcg ccagcgcgtc
      541 gcgcaccgcc tgcgtgaggt cgaccgacac ggcctcggcg ccgatctcgg tgtcggcggt
      601 gaatacagtg cgcgcgttca ggtcgtcggt cgtgacctgg ttgtcgcctt cgccggccac
      661 cacgcgcacc tggagcggaa ccttcggcgg aatgatctcc tcgaacggcg tcgcggcctc
      721 ggggttggag cccgtgaccc agagctggtt gccggcgcgg aagtagagcc ggtcgccgac
      781 cgcggtgagc tgcccgacgc cggtcgccac gttgagcatc ttcttcgtcc cgccgaatgt
      841 gccgtcgctg atgtagagct ctccgttggc gacgaagtac gtccgcaggc cggcctgggt
      901 gtcgaccggc gtgaaggagg acgggaacga gctcgcagtg ccgggcgcga gattgaaagg
      961 cgtcagcacg acgccgctgt gcatgtaaag ctcacgtccg ttcggcccct gcgcggagaa
     1021 gaacagccga tcgccggacg cctcgaggtt gaaaggattc gagctgacga cgttacccgg
     1081 gtcggtcccg atcggaatcg gcgggaacgg cgggaacgtg aagaaaatgg tctgcgccgc
     1141 ctcgtccggc ccgccgatgt tcgccgcgac cgtcgtcggt ccgcccgcga gcgggaccga
     1201 ccagagctcg cgcccgacga acagcggccc ggcggggctc gtgaactccc cgtccgcgtg
     1261 gtcgcgcgcc gcgaaataga tccggttgtt gaagtacgta agctccgacg ggctgaagtc
     1321 ggttacgtcg gtcggcgtga cgttggtgag ctgcgtgaac gcggtgccgc tgtaggtgaa
     1381 gacctggcgg tcgctcaggc cgttcggcag ggcgacgaag gcgagcgttc cgttggcgtt
     1441 gatgagcccg gcgacattcg tgaacggtcc gggcacctgg accggcgtgc ccgattccac
     1501 ccgcacgccc aagtccaccc ggtacagcgc gttcgtggtt ccgttgaagg cggcgaagta
     1561 cagggtgttg ccgaccttcg tcaggtcgaa cgggttcgag ccgttgggac cgctcaggac
     1621 gtcgaacgac gcgggcacgt ccggcgccgc gtcggtttcg tcgacgttga gcacccagag
     1681 ctcggcaccc tggctcgcag ccgccgcgcg gaagtacagc cggtcgccgc tgcgcgcgga
     1741 agtctggccg gcggtgaagc cgagcagctc gccgccatcg atggcgatcg aggtgatgct
     1801 cgcgtccgag ctcacgatcg tgaggatgaa gctcggggac gcatccacgc tcaccacgcc
     1861 ttcgccgagc ccgagctcga ggtttatcgc ctcctcgagc tgaccggcaa gatcgtcgag
     1921 cgagccgttc agggcggtgt cgccgccctc cagcgtgatc gcgagctcct cggtttcgcc
     1981 ggtgctcagg accaccgtga cggtcagcgt gaggtcggcg ggcacctggc cgtccgtcgg
     2041 ccccgccagg gtcgcggtca gcgcgacggt acgggtggag gtctgccctt cagtcaaccc
     2101 caggctggcc aggccggcgg cggtgccggt gccggcgcgc acggtgatcg aagcgatgtt
     2161 cgcatcgccc acggtcagca ccagcgcgcc cgagctgtcg gcgctcacgg tgaccgcgcc
     2221 ggagccgaag ccgttgctga gcagcgccgc gggcaggatg gcgttcaggt cggccgcgag
     2281 gtgctcggcg gtcgtgttgt ccgccgtcac ctcggcggta cgcgggactt ccacgctgat
     2341 cacgtcgccg ccgatcacct cgacgtcgag cgtgaaggaa accgcgccca cgttgggcgc
     2401 ggtgaccggt gcgccggtga gcgagacctg ctgctccgag gcctggttcg cgccgaaacc
     2461 gagcgcttcg ccgcccagca cgcggatgcg cacgatgtct tcatcgagcg cgctgaacac
     2521 gaaacgctcg ccgtccggcg tcgacacgct gacctcgccg ggcaatgctg ccgccacggc
     2581 gttctgaacg tcgagcaaca gcatggagaa gctgccgttc tcggcagtcg ccgcgcgcgg
     2641 cagggtgacc gcctcgctct cgagggtccc gttggcgcgc aggatctcga gcgtgaaggc
     2701 gtaatcgacc gccgggatgc cgtcgctcgg gccggcggtc gcggcggtga gcacggcgct
     2761 gccggaaagc gcggtgaggt tcgcggcgaa gcccgggatg tccgcgacct tctgcgtgcc
     2821 gtccgtcgtg ccgtccgact tccacagctg cgtgcccgcg accccggtat tggcggtgaa
     2881 gtacagcttg ccgccgacgt tgacgaagtt gcccgggaag gagccgtccg cacccgggtt
     2941 gagatccgcg acgaagcgcg tggtctcctc ggtgccttcg gtgacccaga gctcctgtcc
     3001 ttcgcccggg gtcgaggccg agaagaacac caggttgccg accggcagga tcgcgccgat
     3061 gttgaccggc gcctggatcg gtgcggtggt ctgcggcgcg tgcagcacga gctgtgccga
     3121 gccgacggcg tccgggttgc ccgcccagcc gaggaagcgg ccgaggtcga actcgagcac
     3181 cgccccactg gtgaccgaag cattgatcgc gtagtagcgg gtgaaggtct ggatcgggtt
     3241 gagcgtgcgg ttcagctcga ggttctgcgc cgcgccctgg ttggggccga accagccgac
     3301 ggctccgaac tcgtcgccgc tgaaggcgaa ggtgttgaag ccgacggctc cggcgttctc
     3361 gccggcggcg gagccgtcgt accagccgag gcggtagccc gggccgaccg agtcgctgcc
     3421 cgactcgagc tcgaagtcga acgtcagcgt gctgttcggc gcgatccgct cggccttgcc
     3481 gatgccggtg atctggaagc cgcccgccgt ctgcttgaag atgagcggag tgaggtagcg
     3541 atcgtcgccg gcgcccacgc cgtcgacgaa attgccgctg aacaccgacc actgcgtcag
     3601 ccgtccgctc tcctggaacg caccggcgac gaacatgctg ccgacggcgc cgtccacggt
     3661 gttgcgctgg atgagccggt tgccgtcggt ggacggggtg cccgcctcga gcgtcgaggt
     3721 cgtgggcgcg atcgggtcga cgaagccgtc gatcgcgccg aggcccgaca ggatgcggat
     3781 gacgttgccc ggcatgccgt cgccgagcgt ctggaaggtg taccagcgct cgttgccgtt
     3841 atcgagaatg aacttgggct cgtcctcgcc gggcgcgtcc tggaacacct ccacctcgcc
     3901 gaggttcttg ccgaccagga agaagcccgc gccggtgaag gagcggttgc ccagctcgat
     3961 cgcgtccgcg ggcagcgccg cgcgcgacga ggagccgtag atcacgtaga tcttgccgcc
     4021 ttccggtatc acctcggtgg tggtgacgtt cgcggtcgcg gcgccgacca gcaggtcgtc
     4081 gagcccgtcg aggtcgaggt cgaaggccgg ggtcgcgggc agcgcgccga agccgtcgaa
     4141 ctcgaactcg ccgcgcaccc tggcgtcggc ctcggtcagc accttgaccg tcgagccgtc
     4201 ggcgacgcgc gagaacacgt acagcgcgcc cgagtcgtcc tggtcgatga tctgctggcc
     4261 ggtggtcacg aggcgcgcgg tctcgccgac cgcgaggtcg gcatggccgt cgccgtcgaa
     4321 gtcgccgccg gtggcgtgca gcgcgccgtg gaacagcgac ccgggcagca cgtcgtcgcc
     4381 ggctgcgcgc acgatgcgga tgtctgcgtc ccgcgcggaa agcggctcgg catcgaaccc
     4441 ggcctggccg aagtacacga gcaggccggc gtcctggacg gtgtcgcccg ggaacgcctt
     4501 ctggccctcg gcgaggctgc cgacggcgaa gtcgtcgtag ccgtcgaagt tcaggtcgcc
     4561 gagcgccgag acgctgccgc cgagcgagaa gtcctgcacc acgagctccg acttcacccc
     4621 gccgaggaag tcgatcgctt cctgcggcgc atccagccac agcaaggtgg gcgcgccgac
     4681 ccgcccggtg agcacgtaag ccttgccgat gttcgagaag ccctcggtgc cggtgaagtc
     4741 gacgtagccg gagtcggcga gcagcaggtc gtccagcccg tcgcccgtga tgtcgccggc
     4801 ggcgaccgcg cgcacgtggg tgctcagctc ggtgaacgcc gcgagcggct cgccctcggc
     4861 gacgccgaga atttccctcg ccgccatttc gcggtccgtg gtgtcgctga cgatgatccc
     4921 gaggccggtg gtgccgtccg ggtcgagggc atcgaacacc tcgttccaca gcgcgtcgcc
     4981 cgacagcata tagccctgga cgttgaagct cggcgccgac gagcgcacca gcgcgacgtc
     5041 ggcgtggccg tcgtcgttcc agttgagcac cgcgagcgaa gcgctcgggt cggcgaagcc
     5101 ctccggcagg ccggtcgcgg agcggatgcg cacgcgctcc tggttgagaa cgtcgttcgt
     5161 ggcgtcgacc caggcgtagt cgatgacgcg cggcagctcg atgcccgcgc cgttgccgcc
     5221 ggcgatgatg atcagctcga agtcgtcgcc gacggtctgc ttcaggaaca cgagatcgtc
     5281 gagcccgtcg ccggtgaggt cgcccatcct cgaggccggg cggccgacgt cggcggcgat
     5341 gacgatgtcg gcggcctgag cgatgctctc gaccgagtcg agctccaccg gcccgagcag
     5401 cacgtagctc gccttgtcgc cgtaaaccag caggtcgttg aagctgtcgc cgttgaagtc
     5461 ggccatgctg accgagccgg cgagccgctc ctcttcctgg gcgccttcga gcacgaaggc
     5521 gtcgcgcagg ttcggcgtga agtcgttcgc gaggtcgact cccggcagcg ggctcggcac
     5581 gaacccgggc gcggtcgggt tggcgagctc gaagcgccag aggtccggct cctcctcgag
     5641 ccccggcgtc tcctcggtgc ccgtggcctg ctcgagccgc tcgccggggt agagccgcag
     5701 cgcgtcgccg gccgggccgg tgacgccgag cagcgcgcga tcgatgccat tgcggaggcc
     5761 gagggcgccc agatctgcgc cgccgctgcg gatggtaatg gcgaagatgc tcgcgtcgtt
     5821 cgccgacagc tcgagcgcat ccgatccggt cgcggtcacc gcgatcgcgc cggcggca
//
LOCUS       DASPFR010000265         2388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_563774, whole genome shotgun sequence.
ACCESSION   DASPFR010000265 DASPFR010000000
VERSION     DASPFR010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563774"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VD737_07080"
     CDS             complement(<1..254)
                     /locus_tag="VD737_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10360.1"
                     /translation="MNATFAEISILGPALIAGLVVVLSHVPLGQQVLRRGIVFIDLAI
                     AQVAGLGVIAAQSAGLDLHGWKTQVAAVVAALLGALLLTWT"
     gene            complement(259..1182)
                     /locus_tag="VD737_07085"
     CDS             complement(259..1182)
                     /locus_tag="VD737_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HXW10361.1"
                     /translation="MNRSLIRRVVVAAACAVLALPAHAAVKVLATTADWAALTTELGG
                     DKVDVYRATSALQDVHSVEAKPSLVARARTADLLVANGAELEAGWLPVLLQESGNRRI
                     QPGAPGYFEAVSAVKLVDVPVRTDRAMGDIHALGNPHVQLDPRNVAAIARALTTRLAT
                     IDAANAAWYQARGEDFQQRWASAMTRWSAQAAAMKGMPVVVIHRDQRYLARWLGLDEL
                     AAIEPKPGVPPSAGYLAGLVSRLNATPPRAILRNAYNDPKAAEWLAARVHAPVITLPF
                     SVGGTPAAKDLFGLFDDTLARLLAIAPKPQA"
     gene            complement(1193..>2388)
                     /locus_tag="VD737_07090"
     CDS             complement(1193..>2388)
                     /locus_tag="VD737_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10362.1"
                     /translation="RDGATSPDVVPAAPSGTSAFNPAMSVVLTGTYAHLSQDPEDYAI
                     AGFFPGGDEIGPGERNFSLGESEITLSASVDPYFSAAMTFALTGEGELEVEEAFARTT
                     ALPAGLTLKGGRFFSGIGYLNEVHAHAWDFADQPLGYQALFGGQLAQDGVQLKWLAPT
                     DVFLEFGAEAGNGEQFPGTRSDGNGLGTYALFAHLGGDVGESIGWRAGLSWVDADASE
                     RTYEDVDGAGDPVVNTFSGQSRTWLVDATLKWAPGGDARRRSLELQAEYLRREEHGRL
                     AFDLEGAALDGSFRSRQDAWYAQAVYRFAPRWRAGLRYDALDSGDPRIGLVRNSTLAS
                     GDFPLLAPATPSRVTVMADWSPSEFSRLRAQVAWDDARDSGDTDEQWLLQYIHAIGAH
                     GAHKF"
BASE COUNT          334 a          838 c          881 g          335 t
ORIGIN      
        1 gtccaggtga gcagcagggc gccgagcagc gccgcgacga ccgcggcgac ctgcgtcttc
       61 cagccgtgca ggtcgagacc cgcggactgc gcggcgatga cgccgaggcc ggcgacctgg
      121 gcgatcgcca ggtcgatgaa cacgattccg cgtcgcagca cctgctgccc gagcggcacg
      181 tgcgacagca cgacgaccag ccccgcgatc agggccggtc ccaggatgct gatctctgcg
      241 aaggtcgcgt tcacgtggct atgcctgcgg cttcggggcg atcgcgagca gtcgtgcgag
      301 cgtgtcgtcg aacagcccga acaggtcctt cgctgccgga gtgccgccga ccgagaacgg
      361 cagcgtgatc acgggcgcgt gcacgcgcgc ggccagccac tccgcggctt tggggtcgtt
      421 gtaggcattg cgcaggatcg cgcgcggtgg cgtggcgttc agtcgtgaca cgaggcccgc
      481 gagatacccc gcgctcggcg gaaccccggg cttcggctcg atcgcggcga gctcgtcgag
      541 gccgagccag cgcgcgaggt agcgctggtc gcggtggatc accacgacgg gcatgccctt
      601 catggcggcc gcctgcgcgc tccaccgtgt catggccgac gcccagcgct gctggaagtc
      661 ctcgccgcgc gcctggtacc aggcggcatt cgcggcgtcg atcgtcgcga gccgcgtcgt
      721 gagggcgcgc gcgatcgccg cgacgttgcg cggatcgagc tgcacgtgcg ggttgccgag
      781 tgcgtggatg tcgcccatcg cgcgatccgt gcgtacgggg acgtcgacga gcttcacggc
      841 cgaaaccgcc tcgaaatagc cgggcgcgcc cggctggatc ctgcggttgc cggactcctg
      901 cagcagcact ggcagccagc cggcttcgag ttcggcgccg ttggcgacca gcaggtcggc
      961 ggtgcgcgca cgcgccacca ggctcggctt ggcctcgacc gagtgcacgt cctgcagtgc
     1021 gctcgtggcg cggtagacgt cgaccttgtc gccgccgagc tcggtcgtga gggcggccca
     1081 gtccgccgtc gtcgcgagca ccttgacggc cgcgtgcgcg ggaagcgcga gcacggcgca
     1141 tgccgcggcg accacgacgc ggcggatcag tgaacggttc atggcgggct cctcagaact
     1201 tgtgcgcgcc gtgcgcgccg atcgcgtgga tgtactgcag cagccactgc tcgtcggtgt
     1261 cgcccgagtc gcgcgcgtcg tcccacgcga cctgcgcccg caagcgcgag aactcgctcg
     1321 ggctccagtc cgccatcacc gtgacgcggg agggcgtggc gggcgccagc aacgggaagt
     1381 cgccgctcgc gagcgtgctg ttgcggacca ggccgatccg cggatcgccg gagtcgagcg
     1441 cgtcgtagcg caggcctgcg cgccagcgcg gcgcgaagcg atagaccgcc tgcgcgtacc
     1501 acgcgtcctg gcggctgcgg aagctgccgt cgagcgcggc gccctcgaga tcgaacgcga
     1561 gacggccgtg ctcctcgcgc cgcaggtact cggcctgcag ttcgagggaa cgccgacgcg
     1621 cgtcgccgcc gggcgcccac ttcagcgtcg cgtcgacgag ccaggtgcgg gactggccgc
     1681 tgaacgtgtt cacgacggga tcgcccgcgc cgtccacgtc ctcgtaggtg cgttcgctcg
     1741 cgtcggcgtc cacccaggag agccccgcgc gccagccgat cgactcgccg acgtcgccgc
     1801 ccaggtgcgc gaagagcgcg taggtgccga ggccgttgcc gtcgctgcgc gtgcctggaa
     1861 actgctcgcc gttgccggct tcggcgccga actcgaggaa cacgtccgtc ggggcgagcc
     1921 acttgagctg cacgccgtcc tgcgcgagct gtccgccgaa cagcgcctgg tagccgagcg
     1981 gctggtcggc gaagtcccag gcgtgcgcgt gcacctcgtt caggtagccg atgcccgaga
     2041 agaagcgacc gcccttgagg gtcagcccgg ccggcagcgc cgtcgtcctc gcgaacgctt
     2101 cctcgacctc gagctcaccc tcgcccgtca gggcaaaggt catggccgcg gagaagtacg
     2161 ggtcgacgct ggccgacagc gtgatctcgg actcgccgag gctgaagttg cgctcgccgg
     2221 ggccgatttc gtcgccgccg gggaagaagc cggcgatcgc gtaatcctcc gggtcctgcg
     2281 agagatgggc gtaggtgccg gtcagcacca ccgacatcgc cgggttgaag gccgaggtgc
     2341 ccgaaggagc ggccggcacg acgtccgggg acgtggcgcc gtcgcgcg
//
LOCUS       DASPFR010000266         5272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1540739, whole genome shotgun sequence.
ACCESSION   DASPFR010000266 DASPFR010000000
VERSION     DASPFR010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540739"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..727)
                     /locus_tag="VD737_07095"
     CDS             complement(68..727)
                     /locus_tag="VD737_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXW10363.1"
                     /translation="MKTRSTDTADESLAVPAGWQAQKSASTRLQIVESALRCVVDLGY
                     ACTTTTVIADKAGLSRGAMLHHFPSKIDIVRAAVEHLHAKRLKAFRKAIDKLPRDETR
                     VRRALEAYFEHVKHPMYVAFLELWVASRTDPELQAILKPAQEAFEREWYKTAVDLFPE
                     WEGAGENFDIALDLVHYVMDGLAVSLLTHEIGDHERRMIRYLEDRLRELAGRKQAAVE
                     A"
     gene            873..1631
                     /locus_tag="VD737_07100"
     CDS             873..1631
                     /locus_tag="VD737_07100"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXW10364.1"
                     /translation="MGRLDGKVAIVTGGSAGLGRADAIALAREGARVVLTDVNEADGS
                     RVADAINGAQPGSALFLPQDVRDEARWQALIATTVETFGGLHVLVNNAGVVRIATPES
                     CTLEDFRFQNAVMSEGVFLGCKHAIPAMRDSGGGSIINMSSVASHLGYPVYFAYSAAK
                     GAVRSMTKAIAVHCQMNKYNVRCNSIHAGAIDTQMVRDATKTLGMEMSYWEQTPTGLG
                     RPEDVANLVVFLASDESRYVNGAELLLDNALIVQ"
     gene            1740..2501
                     /locus_tag="VD737_07105"
     CDS             1740..2501
                     /locus_tag="VD737_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXW10365.1"
                     /translation="MDLGLRGKKAIVTGATRGIGRAIVDLLASEGVDVGFCARNEDEV
                     NDTTAALKSRGVNAVGACVNVRDGDAYKAWLEKTAADLGGCDIFVANVSAGGGMDSEK
                     NWWKNFEIDVLHTVRGCEALMEHLKKSGQGSVVIISSTNAIETFAAPMAYNAMKAALI
                     TYSKQLSQFVGKFNVRVNSICPGPIYFEGGAWEMIKGTNQKFYDATVRQIPGGRMGTP
                     DEVARVVAFLASPAASLVTGANVVADNGYTKGVRF"
     gene            2511..3257
                     /locus_tag="VD737_07110"
     CDS             2511..3257
                     /locus_tag="VD737_07110"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HXW10366.1"
                     /translation="MGKAAIDAYFVCGGRYHDIDYARLQLLTLLAEQPRVRTRMAEDY
                     RDVAAIEAADFLVTYTVDIVPDEETARRLRNYVSGGKRWLALHGTNSILRYAKGRGWE
                     APRDACTFMETLGSQFVAHPPIAPYRVEVTQPEHPLVAGIEPFDAEDELYLMELHPPF
                     ETLLHTRWSGEAKGFAERDWTRDEPRPVMYLKRVGAGEVLYFTLGHARGHYDMRPLMD
                     EYASVERGSWSVPAFQELLRRGLRWAAGIA"
     gene            3269..4633
                     /locus_tag="VD737_07115"
     CDS             3269..4633
                     /locus_tag="VD737_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HXW10367.1"
                     /translation="MATNTQDDLRTASRTPPYKRVPRVEPGQSPMTPVEKAPDPDLGH
                     DIIRKERYTSFEFMQLEWDRIWTKVWLLGGLESDLREPGDFVCTDIGRESILIVRQAD
                     GGVRAFYNVCLHRGNRLRPAGVGSTDSFKCQYHHWDYALDGSFKRIPDLETFPQGAPP
                     CRGLVEVPCDTWGGFAWFSLDPQVRPLREFLDPMPQHLDPYNFHKMALTRDITVEWNC
                     NWKTSVDAFNESYHVQGIHPQLMWYLHDLDIQIDCYERHNRYLIPFATWSPRVRRPPS
                     IPEPIKVIMKAAGMDPADYDEPLENLRRDVQRHKRKHGSLQGKDYSRLNDDQLTDDYH
                     YLIFPNVTLNVHADDLMLFRQRPHPTDPDRMYYDIWTFELVPEGKEWPDRPRHQHFRH
                     GEKSIGLVLDQDAANLPGVQAGMHSKAFEGLWIGSQELRIRHFHKVIDDYVYGPDGKP
                     PGAI"
     gene            4689..>5272
                     /locus_tag="VD737_07120"
     CDS             4689..>5272
                     /locus_tag="VD737_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HXW10368.1"
                     /translation="MTAAAPLSAERLLRAYRQMKTIREFEERLHVEIATGEIPGFTHL
                     YAGQEAVAVGICEHLADDDYIVSTHRGHGHCIAKGCDVTGMMLEIYGRRDGLCKGKGG
                     SMHIADVTRGMLGANAIVGGGPPLAVGAAIACRNRGDGKVSVGFGGDGSCNQGTVFEA
                     MNMAVVLKVPAIFVFENNGYSEHTGCDYAVGSKD"
BASE COUNT          947 a         1767 c         1761 g          797 t
ORIGIN      
        1 ggtgcgggag aggtggcccc tcacccgcgc ttcgcgcgac ctctcccgcg gtgcgggagg
       61 tggagactca ggcttcgacg gcggcctgtt tcctgccggc gagttcgcgc aggcggtctt
      121 ccaggtaacg gatcatgcgt cgctcgtgat caccgatctc gtgcgtgagc aggctgacgg
      181 cgagcccgtc catcacgtag tggaccaggt cgagggcgat gtcgaagttc tcacccgccc
      241 cttcccactc gggaaacagg tcgacggccg tcttgtacca ctcgcgctcg aacgcctcct
      301 gcgcgggctt caggatcgcc tgcagttcgg gatctgtacg cgaggcgacc cagagttcca
      361 ggaaagcgac gtacatcggg tgcttgacgt gctcgaagta ggcctcgagc gcgcggcgaa
      421 cgcgcgtctc gtcgcgcggc agcttgtcga tcgccttgcg aaaggccttc agccgcttgg
      481 cgtgcaggtg ctcgacggcg gcacggacga tgtcgatctt ggagggaaag tgatgcagca
      541 tcgcaccgcg cgacagcccg gccttgtcgg cgatcacggt ggtggtcgtg caggcgtaac
      601 cgaggtcgac gacgcagcgc agcgccgact cgacgatctg caaccgcgtc gaagcgctct
      661 tctgggcctg ccagcccgca gggacggcca ggctctcgtc cgcggtgtcg gtgctgcgag
      721 tcttcatgaa acggcttccc caaacggtac gagcaagaat gtggcgcctg tcgggaaaag
      781 tcaaagatga ttgaaaccga cagggttgac tgctaacgtc ggccgcgccc cgccgcgggg
      841 gcgcgcgttt tcacacttcg ggggatccgg acatggggcg actcgacggc aaggtggcga
      901 tcgtcacggg cggctcggcg ggcctgggac gggccgatgc catcgcactt gcacgcgagg
      961 gcgcccgggt ggtgttgacc gacgtcaacg aggcggacgg atccagggtc gcggacgcga
     1021 tcaacggcgc gcagccggga tccgccctgt tcctgccgca ggacgtgcgc gacgaagcgc
     1081 gctggcaggc cctgatcgcc acgaccgtgg aaacgttcgg cgggctgcac gtgctcgtga
     1141 acaacgccgg ggtcgtccgg atcgcgaccc cggaaagctg cacgctcgag gacttccgat
     1201 tccagaacgc cgtgatgtcc gaaggggtct tcctgggctg caaacacgcg attccggcca
     1261 tgcgtgacag cggcggtggt tcgatcatca acatgtcgtc cgtcgcctcg cacctcggct
     1321 accccgtcta cttcgcgtac agcgccgcca agggcgcggt gcggtcgatg accaaggcca
     1381 tcgccgtgca ctgccagatg aacaagtaca acgtgcgctg caactcgatc cacgcgggcg
     1441 ccatcgacac gcagatggtg cgcgacgcga cgaagacgct cggcatggag atgtcgtact
     1501 gggagcagac gccgaccggc ctcggcaggc cggaagacgt cgccaacctg gtcgtgttcc
     1561 tggcgtccga cgaatcgcgc tacgtgaacg gggccgagct gctgctcgac aacgcgctga
     1621 tcgtccagtg acgacgggcc gtctcaacac aaaaaaacag tcaagactgt ttaattcaac
     1681 ccgacggctg actatactgc ggtggcagca gccacgcgcc gtcacctgga gtacgcacga
     1741 tggatctcgg actgagaggc aagaaggcga tcgtgaccgg cgcgacgcgt ggcatcgggc
     1801 gggcgatcgt ggacctgctg gcgtcggaag gcgtcgacgt cggcttctgc gcgcgcaacg
     1861 aggacgaggt caacgacacc acggccgccc tcaagtcgcg cggcgtcaac gcggtgggcg
     1921 cctgcgtcaa cgtccgcgac ggcgacgcct acaaggcctg gctcgagaag accgcggccg
     1981 acctcggcgg ctgcgacatc ttcgtcgcca acgtgagcgc cggcggcggc atggacagcg
     2041 aaaagaactg gtggaagaac ttcgagatcg acgtgctgca cacggtgcgc ggctgcgagg
     2101 cgctcatgga gcacctcaag aagtcgggcc agggttcggt cgtgatcatc agctcgacca
     2161 acgcgatcga gacgttcgcc gccccgatgg cctacaacgc gatgaaggcc gcgctgatca
     2221 cctactcgaa gcagctctcg cagttcgtcg gcaagttcaa cgtgcgcgtg aactccatct
     2281 gccccggccc gatctatttc gagggcggcg cctgggagat gatcaagggc accaaccaga
     2341 agttctacga cgcgaccgtg cgccagatcc ccggcgggcg catgggcacg cccgacgaag
     2401 tcgcgcgcgt cgtcgcgttc ctggcgagcc ccgcggcgag cctcgtgacg ggcgcgaacg
     2461 tcgtcgccga caacggctac accaagggcg tgcggttctg agccacggcc atgggcaagg
     2521 cggcgatcga cgcttacttc gtctgtggcg gccgctacca cgacatcgac tacgcccgac
     2581 tgcaactgct cacgctgctc gccgagcagc cgcgcgttcg cacgcgcatg gccgaggact
     2641 accgcgacgt cgccgcgatc gaggcggcgg atttcctcgt cacctacacc gtcgacatcg
     2701 tccccgacga ggagaccgcg cgacggctgc gcaactacgt gagcggcggc aaacgctggc
     2761 tggcgctgca cggcaccaac tcgatcctgc gctacgcgaa agggcgcggc tgggaagcgc
     2821 cgcgtgacgc gtgcacgttc atggaaaccc tcggcagcca gttcgtcgcg catcctccga
     2881 tcgcgccgta ccgcgtcgag gtcacgcagc ccgaacaccc gctggtcgcc ggcatcgagc
     2941 cgttcgacgc cgaggacgag ctgtacctga tggaactgca cccgccgttc gagacgctcc
     3001 tgcacacgcg ctggagcggc gaggcgaagg gcttcgccga gcgcgactgg acgcgggacg
     3061 agccgcggcc ggtcatgtac ctgaagcggg tcggtgccgg cgaggtcctg tatttcacgc
     3121 tcggtcacgc gcgcggccac tacgacatgc gcccgctgat ggacgagtac gcctcggtcg
     3181 agcgcggcag ctggtcggtg ccggcttttc aagaactgct gcggcgcggt ctgcgctggg
     3241 ccgcgggcat cgcctgagcg aggcggacat ggctacgaac acgcaagacg atctcaggac
     3301 cgcatcacgg acgccgccct acaagcgcgt gccgcgcgtg gagcccgggc agagtccgat
     3361 gacgccggtc gagaaggcgc ccgaccccga cctcggccac gacatcattc gcaaggagcg
     3421 ctatacctcg ttcgagttca tgcagctcga gtgggacagg atctggacca aggtgtggct
     3481 gctcggcgga ctcgagtccg acctgcgcga gcccggcgat ttcgtctgca ccgacatcgg
     3541 tcgcgagtcg atcctgatcg tgcgccaggc cgacggcggc gtgcgcgcgt tctacaacgt
     3601 ctgcctgcac cgcgggaatc gactgcgccc cgcgggcgtc ggcagcaccg attcgttcaa
     3661 gtgccagtac caccactggg attacgcact cgacggatcg ttcaagcgca tccccgacct
     3721 ggagacgttt ccgcagggcg ccccgccctg ccgcgggctc gtcgaggtgc cgtgcgacac
     3781 gtggggcggc ttcgcctggt tcagcctgga cccgcaggtg cggccgctgc gcgagtttct
     3841 cgacccgatg ccgcagcacc tcgacccgta caacttccac aagatggcgc tcacgcgtga
     3901 catcaccgtc gagtggaact gcaactggaa gacgtcggtc gacgcgttca acgagagcta
     3961 tcacgtccag ggcatccacc cgcagctgat gtggtacctg cacgacctcg acatccagat
     4021 cgactgctac gagcgccaca accgttacct gatcccgttc gccacctgga gcccgcgcgt
     4081 gcgccgcccg ccgtcgatcc cggagccgat caaggtgatc atgaaggcgg cgggcatgga
     4141 cccggccgac tacgacgagc cgctcgagaa cctccgtcgc gacgtgcagc gccacaagcg
     4201 caagcacggc tcgctacagg gcaaggacta ctcgcggctc aacgacgacc agctcacgga
     4261 cgactaccac tacctgatct tcccgaacgt cacgctcaac gtgcacgcgg acgacctgat
     4321 gctgttccgc cagcggccgc acccgacgga cccggaccgc atgtactacg acatctggac
     4381 gttcgagctc gtgcccgaag gcaaggagtg gcctgacagg ccgcgtcacc agcatttccg
     4441 gcacggcgag aagtcgatcg ggctcgtgct cgaccaggac gcggccaacc tgccgggcgt
     4501 gcaggcgggc atgcactcga aggccttcga ggggctctgg atcggatcgc aggagctgcg
     4561 catccggcac ttccacaagg tcatcgacga ttacgtctac ggaccggacg gcaagccgcc
     4621 cggcgcgatc tgatccacga actgcgattc agccgtgcgc ggtcccgcgc gcggccgagt
     4681 gaggcgacat gactgctgct gcacccctct cggcggagcg actgctccgc gcttaccgcc
     4741 agatgaagac gatccgcgag ttcgaggagc gactgcacgt cgagatcgcg accggcgaga
     4801 tcccgggctt cacgcacctc tacgccggcc aggaagccgt tgcggtcggc atctgcgagc
     4861 acctcgccga cgacgactac atcgtcagca ctcaccgcgg gcacggacac tgcatcgcga
     4921 agggctgcga cgtcaccggc atgatgctcg agatctacgg ccgccgcgac gggctctgca
     4981 agggcaaggg cggctcgatg cacatcgcgg acgtcacgcg cggcatgctg ggagccaacg
     5041 ccatcgtcgg cggcggtccg ccgctcgcgg tcggcgcggc gatcgcctgc cgcaaccggg
     5101 gcgacggcaa ggtcagcgtc ggtttcggcg gcgacggcag ctgcaaccag ggcaccgtgt
     5161 tcgaagccat gaacatggcc gtcgtgctca aggtgccggc gatcttcgtg ttcgagaaca
     5221 acggttacag cgagcacacc ggctgcgact acgccgtggg cagcaaggac at
//
LOCUS       DASPFR010000267         2634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1297525, whole genome shotgun sequence.
ACCESSION   DASPFR010000267 DASPFR010000000
VERSION     DASPFR010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297525"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1924
                     /locus_tag="VD737_07125"
     CDS             122..1924
                     /locus_tag="VD737_07125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HXW10369.1"
                     /translation="MSANVVEDRPKARSLGPLRALVPYLRPYGTMLALALGALLVAAA
                     AMLALPVALRYLIDEGLTASDTETINRYFVAFLAAAAVYGVFAALRFYLVTWIGERVV
                     ADMRSAVYARVIRMDPAFFEVTRIGEVLSRLTADTTLVQAIAGVNLSITLRSMLSLVG
                     SLVMLAVTSAELTAMLVVLIPLVIVPLIVLGRRVRRLSRTSQDRIADTSGVADETLNA
                     IQTVQAFTLEDINTRRYDAAVEDSFVAAVRRTRTRSTLTALATMLVFGAITFVLWTGA
                     HAVVRGEMTGGQLGQFLLYAGYVAVAAASLSEMWGEVQRAAGAMERLVELQHAQPMIQ
                     PPARPVPLPQPGRGEIRFDHVTFHYPSRPEAKALDDFELTVRPGETVAFVGPSGAGKS
                     TTFQLLLRFYDPAAGRILIDGVDLAHADPHQVRRRIGLVPQDTVLFAASARENIRYGR
                     PDASDGDVEAAARAAGADEFLRRLPEGYDTFLGERGTRLSGGQRQRIAIARAILRDPP
                     ILLLDEATSALDAESERLVQGALDRLMQGRTTIVIAHRLATVLEADRIVVMDHGRIVA
                     QGTHAELLRGNELYARLAALQFTDGAGSSARPAA"
     gene            1942..>2634
                     /locus_tag="VD737_07130"
     CDS             1942..>2634
                     /locus_tag="VD737_07130"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HXW10370.1"
                     /translation="MTDPSPRLDAWRAPPVPARVILHGRSCRVEPLAMEHAAALHEAN
                     SIDAQGRMWDYLAYGPFDGLDAYRAWVGQKVSSVDPLFFAIVDRAGDAPVGVASYLRI
                     DPPNGVIEVGHLAYSPRLQRTPAATEAMYLMMRHAFELGYRRYEWKCNARNEPSRSAA
                     ERLGFTFEGVFRQHMIVKGRSRDTAWYSVIDGEWPRVRAGFERWLDAGNFDAAGRQRR
                     TLADCRMDSGGVS"
BASE COUNT          365 a          933 c          927 g          409 t
ORIGIN      
        1 tagcggttag tgatcagtcg gagatggacg ccgtcgtgca ctcgacccct cttgctaacc
       61 gctaaccgct aaccgctaac cgctaaccgc taaccactga ccgctgaccg ctaaccgctc
      121 catgtccgcc aacgtcgtcg aagaccgccc caaagcccgt tccctcgggc cattgcgtgc
      181 gctcgtcccg tacctgcggc cgtacggcac catgctggcg cttgcgctgg gggccttgct
      241 ggtggccgca gcggcgatgc tcgcgctgcc ggtcgctctg cgttacctga tcgacgaggg
      301 cctcaccgcc agcgacaccg agacgatcaa tcgctacttc gtcgcgttcc tcgccgccgc
      361 cgccgtgtac ggcgtgttcg cggcgctgcg cttctacctc gtgacctgga tcggcgagcg
      421 ggtcgtcgcc gacatgcgct ccgcggtata tgcgcgcgtg atccggatgg acccggcgtt
      481 cttcgaggtc acgcggatcg gggaggtgct ctcgcgcctg accgcggaca cgacgctggt
      541 gcaggccatt gcgggcgtga acctctcgat cacgctccgc tcgatgctgt cgctggtcgg
      601 cagcctcgtg atgctcgcgg tcaccagcgc cgagctcacg gcgatgctgg tggtactgat
      661 tccgctcgtg atcgtcccgc tgatcgtgct gggccggcgc gtccggcggc tctcgcgcac
      721 ctcgcaggac cgcatcgcgg acacgagcgg cgtggccgac gagacgctga acgcgatcca
      781 gaccgtacag gcgttcacgc tcgaggacat caacacgcgg cgctacgacg cggccgtgga
      841 ggactccttc gtggcggcgg tgcgtcgaac gcgcacgcgc tcgacgctga ccgcgctcgc
      901 gacgatgctc gtgttcggcg cgatcacgtt cgtgctctgg accggcgccc acgcagtcgt
      961 gcgcggcgag atgaccggtg ggcagctcgg ccagttcctg ctctacgcgg gctacgtcgc
     1021 cgtggcggcc gcgagcctga gcgagatgtg gggcgaggtg caacgggccg cgggcgcgat
     1081 ggagcggctg gtcgagctcc agcatgccca gcccatgatc cagccgcccg ctcgaccggt
     1141 gccgctgccg cagcctggcc gcggcgagat ccggttcgac cacgtgacgt tccactaccc
     1201 gtcgcggccc gaggcgaagg cgctcgacga tttcgagctg acggtgcgtc ccggggaaac
     1261 ggtcgcgttc gtcggtccgt ccggcgcggg caagagcacc acgttccagc tcctgctgcg
     1321 gttctacgac cctgcggcgg gccggatcct gatcgacggc gtggacctgg cccacgccga
     1381 cccgcaccag gtccgccggc ggatcggcct ggtgccgcag gacacggtgc tgttcgcggc
     1441 gagcgcacgg gagaacatcc gctacggccg accggacgcg agcgacggcg acgtcgaggc
     1501 cgcggcccgc gccgccggcg ccgacgagtt cctgcggcga ctgcccgaag gctacgacac
     1561 gttcctgggc gagcgcggca cgcggctctc cggcggtcag cgccagcgca tcgcgatcgc
     1621 ccgcgcaatc ctgcgcgatc cgccgatcct gctgctcgac gaagcgacga gcgcgctcga
     1681 cgccgagagc gagcgactcg tgcagggcgc gctcgatcgc ctgatgcagg ggcgcacgac
     1741 gatcgtgatc gcgcaccgcc tcgcgaccgt gctcgaggcg gaccgcatcg tggtgatgga
     1801 ccacggccgc atcgtcgccc agggcacgca cgccgagttg ctgcgcggca acgagctcta
     1861 cgcccggctc gcggcgctgc agttcaccga cggtgccggc tcgtcagcac ggcctgcggc
     1921 ctgatgggcg aggtgagcga cgtgacggac ccatcgccgc ggctcgatgc ctggcgcgcg
     1981 ccgcccgtgc ctgcgcgagt gatcctgcac ggtcgctcgt gccgggtgga accgctcgcg
     2041 atggaacacg cggcggcact gcacgaggcc aactcgatcg acgcgcaagg gcgcatgtgg
     2101 gactacctcg cgtacggccc gttcgacggc ctcgacgcgt accgcgcgtg ggtcgggcag
     2161 aaggtgtcgt cggtcgaccc gctgttcttc gcgatcgtcg accgtgcggg cgacgctccg
     2221 gtcggcgtcg cgtcgtacct gaggatcgat ccgccgaacg gcgtgatcga ggtcggccac
     2281 ctggcctact cgccccggtt gcagcgcacg ccggccgcga ccgaggcgat gtacctgatg
     2341 atgcggcacg ccttcgagct cggctaccgt cgctacgagt ggaagtgcaa cgcgcgcaac
     2401 gagccctcgc ggtcggcggc cgagcggctc ggcttcacgt tcgagggcgt gttccgccag
     2461 cacatgatcg tcaagggacg cagccgcgac acggcgtggt actcggtgat cgacggcgag
     2521 tggccgcgcg tgcgcgcggg cttcgagcgc tggctcgatg ccggcaattt cgacgcggct
     2581 gggcggcagc gtcgcacgct cgccgactgc cgcatggatt ctggaggcgt ctcg
//
LOCUS       DASPFR010000268        13776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1174914, whole genome shotgun sequence.
ACCESSION   DASPFR010000268 DASPFR010000000
VERSION     DASPFR010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13776
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174914"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VD737_07135"
     CDS             <1..262
                     /locus_tag="VD737_07135"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HXW10371.1"
                     /translation="GILDSKPAGPCTQRFPIYSTSRIVAGAPIEGGIYKCALKPVEQA
                     VMDGTYAPWVPTAGDVATLKQTFPSGVCDYSKPDQARPDWLM"
     gene            465..1748
                     /gene="serS"
                     /locus_tag="VD737_07140"
     CDS             465..1748
                     /gene="serS"
                     /locus_tag="VD737_07140"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HXW10372.1"
                     /translation="MLDARLLRSDPDLVAANLARRGFQLDVARLRALEERRKAAQVAA
                     DEVRAARNAHARSVGKAKAQGQDIAGLLAEGEALGRRLTGLEQELAAVQAEFEELVLG
                     LPNLLHESVPDGRDESSNIEVRRWGTPRTFEWTPLDHVAIGERIAGGAMDFEAAGRIS
                     GARFVVLKAGLARLHRALIQYMLDLHTGEHGYTEVYAPYLVARSSLVGTGQLPKFEAD
                     LFKAVSGEAEFFLIPTAEVPVTNLVRESILEADTLPRRYAAHTPCFRSEAGSYGKDTR
                     GMIRQHQFEKVELVHVCRPEASYDELERLVGHAEKVLQGLELPYRVVALCSGDVGFGS
                     AKTYDLEVWLPGQQKYREISSCSNCEAFQARRMQARWRNPATNKPEPVHTLNGSGLAV
                     GRALVAVLENYQQGDGSVTIPAVLRKYMDGAEVLR"
     gene            1779..1868
                     /locus_tag="VD737_07145"
     tRNA            1779..1868
                     /locus_tag="VD737_07145"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1813..1815,aa:Ser,seq:tga)
     gene            2125..3303
                     /locus_tag="VD737_07150"
     CDS             2125..3303
                     /locus_tag="VD737_07150"
                     /inference="COORDINATES: protein motif:HMM:NF028240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HXW10373.1"
                     /translation="MSRHASTGAVALALVFPLTALAQGNPELEELKAQVAALTRKIAE
                     MEARETQKVVAAEPVVQEAKAPTSPNWSDRISVTGDFRYRFDGTDDEGKEYVGLQRLR
                     GRLFVDGKVNDTTNVVMQLGTGGFNPRSQDATLSGDGSSKEVTLRQAYVNWRPMADLA
                     LTAGKMPVPWVVNPVDYFHDTDYFPEGIAVKYGAATGFHASGFWLQIAERGGDDETTM
                     LGTQLGYASDLFFANVAYQDFQKIEGYNPCYNGNCNGNTVDLGGNLVNDFNLLQVRGG
                     VTLAGVSLFASWAQNLEADDEDTAYSVGFNYGKVKDPGSWSAGALYQDVEKDALYGGM
                     FDGTFAGGRTAHDGYVLSGAYGFSKNWSGSLLWFINEVDKTGNPHDYDRYQFDLLWKF
                     "
     gene            3322..4029
                     /locus_tag="VD737_07155"
     CDS             3322..4029
                     /locus_tag="VD737_07155"
                     /inference="COORDINATES: protein motif:HMM:NF038017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXW10374.1"
                     /translation="MQDFGDSFGLAFQLVAAGDSQLLEIILLSLRVSLTAVAIACLIG
                     MPLGAALAVSRFPGRPAVLVLVNALMGQPPVVVGLVIYLLLSNAGPLGWLQLLYTPTA
                     MIIAQIVLITPIVAALSREVVEQLYVECAEHFRSLCLTRIQAVRALLWDARFSLITVG
                     LAGFGRAIAEVGAVIIVGGNIAHLTRVMTTAIALETSKGDLALALALGLVLLTLALSV
                     NAAVMMLRMMDDGHGHH"
     gene            4016..4780
                     /locus_tag="VD737_07160"
     CDS             4016..4780
                     /locus_tag="VD737_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXW10375.1"
                     /translation="MATIDPPFVTAAGTVPASPPGISGAARQLSYRVGDANRVDDVDL
                     DLSPSSLTVVMGPNGAGKSLLLRLLHGLIEPTGGGVYWGGRPLDESLRRRQAMVFQRP
                     VLLRRSVAANVRFVMRLRGLDSVERVRDALDEVGLADRARQPARLLSGGEQQRLALAR
                     ALAIEPQVLFLDEPTANLDPFSMAAIESIIARVHERGTKIVLVTHDLGQARRLADDVI
                     FLNRGRLIEHSPAERFFEHPSSTQARDYLAGRLVLT"
     gene            4808..5638
                     /locus_tag="VD737_07165"
     CDS             4808..5638
                     /locus_tag="VD737_07165"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXW10376.1"
                     /translation="MSERLITGLLSMALLAATATPMSTLAQETSILVQSTTSTQNTGL
                     YEYLLPKFTAKTGIGVNVVAVGTGQAIKNAMNGDGDVLLVHDKPAEEKFVAEGYGVER
                     FDLMYNDFVVVGPANDPAGIAGMKDTEAALKKLAGKKSLFASRGDNSGTHSKELALWK
                     AAGIDPAAASGTWYRETGSGMGATLNTAVGMGAYALTDRGTWISFKNKGDYRIVIEGD
                     PDLFNQYGVILVNPARFPNVKAKPGQAFVDWLISDEGQAAIAGYRIEGQALFFPNARK
                     "
     gene            complement(5700..5972)
                     /locus_tag="VD737_07170"
     CDS             complement(5700..5972)
                     /locus_tag="VD737_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10377.1"
                     /translation="MSRRTELSGYDAQQAVRILELCSQFQQLRRSYAENDDPLALAEG
                     MRQVDLTALDEAHAWLTRLLVDRYAAERITLDTGKFRIPAFNPLKA"
     gene            6268..8052
                     /locus_tag="VD737_07175"
     CDS             6268..8052
                     /locus_tag="VD737_07175"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HXW10378.1"
                     /translation="MPQTRSQTDASRTRPHLPMQGIAWAGLLLTLIFTLGYWRYTDLD
                     LDRRVDQHFRQLAQRQVDALVERMHAHERVLRGASALFAASTAVSREEWRAYIDNLDL
                     ELTLPGAQGTGFAAVVPRDEREAHEQAVRAEGFADYAIEPAGDRPVYSSIVYLEPFDD
                     RNLRAFGYDMYSEPTRRVAMDRARDTGGPAMTHKVTLVQETDEDVQAGFLIYVPVYAA
                     NQPLRSVEERRAALVGWVYSPYRAADLMRSVFQGFNGDAEVQVFDGEPEPHNLLYATR
                     DAARAAEHEATFKVEIGGIPWTARFRSSAAFDAGIDRLQSRLVLLAGVLLSMLVFSLL
                     YAETRHRAGLEAQVRERTLELEKARDEAEGASRAKSAFLATVSHELRTPLNAIIGFSS
                     ILLQDSLSSEQRKQLGIINRSGLQLLDLIKEILDITSIEAGHLAMHLEPVNLRQLLEE
                     QCEVLQMQARDNGLYLKLAECEPPVIVVADQGRLSQVVRNLVSNAIKFTDRGGVTVRC
                     RTVGDTAMVEVEDTGIGIPQEAHATLFVPFQRGDRHGGRPGTGLGLAISRRLVEAMGG
                     QIGFESVVGQGSRFWFTVPRSRTERRAA"
     gene            complement(8057..9709)
                     /locus_tag="VD737_07180"
     CDS             complement(8057..9709)
                     /locus_tag="VD737_07180"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HXW10379.1"
                     /translation="MSARRFGPRETVDFVVVGSGAAGGVMARELARAGSTVLVLEQGS
                     RLTAADFTHDEFDVWLNSGLINKLSTNPQSFRRSEAETAVAVRDFPCAWYAPTVGGSS
                     THFTGNFWRFHEVDFDERSRLGPIAGTTFDDWPITYAELEPYYTRVEWEVGVSGLAGA
                     SPFDPPRSKPYPMPPLPVKSSGVLFERGARKLGLHPFPAPMAIASEPFRDRPACQHCG
                     FCMGYGCEMRAKSSTLYTMLPEAEATGRCEIRPHSYVTRIETGPKDRVTGVAYFDAAR
                     REHFQKARAVVLCANGAESARLLLLSANARHPDGLANSSGMVGRNLMFNTYAQVHALF
                     EHELNEFKSVQVTRVLHDYYETDPARGCYGGGGLDARSGPMPIVWGAFAPPPGRRWGA
                     ALKSLLEAMPRAMVVATHGTSLPLASNRIDLDPALKDAWGLPALRMTYRDHPDDLAFA
                     RFLQDRGVEIMQAAGALDVWKDQVTEAVGSPHLLGTARMGDDPRTSVVDRFHRAHDVR
                     NLFVCDGSSFVTSGRGQPTMTIMALAFRAGEHIARFARRGEV"
     gene            complement(9706..10329)
                     /locus_tag="VD737_07185"
     CDS             complement(9706..10329)
                     /locus_tag="VD737_07185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HXW10380.1"
                     /translation="MDSNELSRRRFLQSSGGSLGFAWLAANWPQIVAAAEHAHAASAA
                     DGGDRSRFRLFDARQARDVEAIAAQIVPGGDTPGAREAAVVYFIDQVHAGPWSGGATQ
                     FLEGLRDFQARCARHHPGTAQFADLSAADQLAYLQHVERTPFFEQVRFLTVVGLLASP
                     RYGGNAQKAGWKLVGFVDQHAWQPPFGHYDAGYQGFTPYSGTPGKRT"
     gene            10457..10831
                     /locus_tag="VD737_07190"
     CDS             10457..10831
                     /locus_tag="VD737_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013346237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXW10381.1"
                     /translation="MSQPNPVRWFEIYVQDMARARRFYETVLDTTLTRLDSGGPEMWA
                     FPSTQGEYGVGGTLVKIDGVRSGGNSTLVYFGCDECSVEAGRIVGAGGTVQRERMSIG
                     PYGYVVLALDTEGNMIGLHSTR"
     gene            10890..11744
                     /locus_tag="VD737_07195"
     CDS             10890..11744
                     /locus_tag="VD737_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10382.1"
                     /translation="MTRQSQTFTIATIVGVSAIMLVACGRAPTGSVDAAGVSPAPEVA
                     TLDADQPTGAARVVTAAGTAPQPGDELRRREAELAARERELRVQQDELRRERERLEAA
                     RGGGRPSTSASDDSFDADQATAPAATTPAHSSIQKVPTPPIVVPPGTPLKIELAANVD
                     TRVARVGDRVEGRLASDLVVGERRAAVAGAAVTGRVTELVPGRDEAGGLPTLVVTFES
                     LQAANGATVPIVAKFRQQDDSDMAREAAEGAGGAAFVAAAQRTGGEVRIRAGTVITAP
                     TETSLSIY"
     gene            complement(11754..12329)
                     /locus_tag="VD737_07200"
     CDS             complement(11754..12329)
                     /locus_tag="VD737_07200"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXW10383.1"
                     /translation="MKRTAWIYAAATLAIAAASALSLAHAQQAETQAPTAPQTLPGTT
                     SPAATSKVTAEAKAEAVVPDPARTPLAIKVNDPNLKWAPCPPLFAEGCRIAVLHGDPA
                     KPGVDLFFQVPGGYAIAPHSHTSAERMVLVGGELEVTYQGRSPIVLQTGQYAYGPAEA
                     PHSAKCRSQDPCTLFIAFDLPVDAIPYDGKL"
     gene            12420..12869
                     /locus_tag="VD737_07205"
     CDS             12420..12869
                     /locus_tag="VD737_07205"
                     /inference="COORDINATES: protein motif:HMM:NF020413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1810 domain-containing protein"
                     /protein_id="HXW10384.1"
                     /translation="MTDRISDPWRLSRFVEAQSGVYERALAELRDGRKRSHWMWFVFP
                     QLAGLGFSAMSVRYAVSGLAEARAYLDHPVLGPRLRECAAALLALDERCSAAAVMEYP
                     DDLKLRSSATLFAQVSSPGSVFHQLIDRYFGGKPDEVTLKLATAGDA"
     gene            12977..13531
                     /locus_tag="VD737_07210"
     CDS             12977..13531
                     /locus_tag="VD737_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HXW10385.1"
                     /translation="MAQALATPGSSPVLRGRVIDVIDGDSVAVQLQTGRIEVRLHAAD
                     APEHDQPGGRAAGRALRKRLPRGSDVLLEPFEQDQYDRLVAVIEQDGESVNAWMVEQG
                     HAWAYRQYTTDARYCRWEDAARAAHRGLWSSPPGEWIAPWDWRRRAREPGYRPADRSR
                     ETLADCRASLRHRRNAATGARTPR"
BASE COUNT         2194 a         4673 c         4837 g         2072 t
ORIGIN      
        1 tggcatcctg gacagcaagc ccgcaggtcc ctgcacgcag cgattcccca tctattccac
       61 gtcgcgcatc gtcgcgggtg cgccgatcga gggcggcatc tacaagtgcg cgctcaagcc
      121 ggtcgagcag gccgtcatgg acggcaccta cgcgccgtgg gtgccgaccg cgggcgacgt
      181 ggcgacgctg aagcagacct tcccgtcggg cgtctgcgac tactccaagc ctgaccaggc
      241 acgccctgac tggctgatgt gagtctccgg ccacctctcc cgcgccggcg ggagaggtcg
      301 gccgaaggcc gggtgagggc gcggagcctc ttccacgcgg cggcgccgtc cccggatcga
      361 aatccgcggc cggcaccacc gctgcatcgc gttgacgtca ccgtcatcag gagggcagcg
      421 gccgcctgct acaattcgcg gctttccgcc cgcgaattca ttccatgctc gacgcccgac
      481 tgctccgctc cgaccccgac ctggtcgctg ccaacctggc gcgtcgcggc tttcagctcg
      541 acgtcgcgcg cctgcgcgcg ctcgaggagc ggcgcaaggc ggcacaggtc gcggcggacg
      601 aagtgcgcgc ggcccgcaac gcgcacgccc ggagcgttgg caaggcgaag gcgcagggac
      661 aggacatcgc cgggttgctc gccgagggcg aggctctcgg acggcggctc acggggctcg
      721 agcaggaact cgcggccgtg caggccgagt tcgaagaact ggtgctcggg ctgccgaacc
      781 tgctgcacga gagcgttcca gacggccggg acgagagttc caacatcgag gtgcgacgct
      841 ggggcacgcc gcgcacgttc gagtggacgc cgctcgacca cgtcgcgatc ggcgagcgga
      901 tcgccggcgg cgcgatggac ttcgaggcgg cgggcaggat ctcaggcgcc cggttcgtcg
      961 tgctcaaggc cggactcgcg cggctccatc gcgcactgat ccagtacatg ctcgacctgc
     1021 atacgggcga gcacggctat accgaggtgt acgcaccgta cctcgtcgca cgcagctcgc
     1081 tggtcggcac gggccagctg ccgaagttcg aggccgacct gttcaaggcg gtgtccggcg
     1141 aggcggagtt cttcctgatc ccgaccgccg aggtgcccgt gacgaacctg gtgcgggaat
     1201 cgatcctcga ggcggacacg ctgccgcgcc gttatgccgc gcacacgccg tgcttccgga
     1261 gcgaggccgg ttcctacggc aaggacacgc gcggcatgat ccgccagcac cagttcgaga
     1321 aggtcgagct cgtgcacgtg tgccggccgg aggcgtcgta cgacgaactc gaacggctcg
     1381 tgggccacgc cgagaaggtg ctgcagggac tcgagctgcc gtatcgcgtg gtcgcgctct
     1441 gcagtggcga cgtgggcttc ggcagtgcca agacctacga cctggaggtg tggctgccgg
     1501 gccagcagaa gtaccgcgag atctcctcgt gctcgaactg cgaggcgttc caggcgcgac
     1561 gcatgcaggc gcgctggcgc aacccggcga cgaacaagcc cgagccagtg cacacgctga
     1621 acgggtcggg cctggccgtg ggccgcgcgc tcgtcgccgt gctcgagaac taccagcagg
     1681 gcgatggcag cgtcacgatc ccggccgtgc tgcgcaagta catggacggc gccgaggtcc
     1741 tccgctaggg ctcggcgcgc cttaccatgg tccgccccgg agaggtggca gagcggttga
     1801 ttgcaccggt cttgaaaacc ggcgtcgcct gacggcgacc gagggttcga atccctccct
     1861 ctccgccacg tccggcgcgt ggcgattcgt cgccgccctc atcccatgac ttttccgcag
     1921 cgcccgcgtc gcgccatcgc acgcggaccg ccccatcgaa gacagatcac cggcgtcggc
     1981 gccggtcaat gtcgctttta cagattcttt ggtgtttggc ttcctggtat gttgaaattt
     2041 ccttatcacg ttcgcactgg tctaccccga ggacccgaga ggtgtggctg cctcgccttc
     2101 agggaaaaag cacaggaacc acacatgagc agacacgcat cgaccggcgc ggtggccttg
     2161 gccctcgtgt tcccgctgac cgcactcgcc cagggcaatc cggagctcga agagctcaag
     2221 gcgcaggtcg cggcgctcac ccggaagatc gccgagatgg aggctcggga aacacagaag
     2281 gtcgtggccg cagagcctgt cgtgcaggag gcgaaggccc caacgtcgcc gaactggtcg
     2341 gaccggattt ccgtcaccgg cgatttccgc taccgcttcg acggcaccga tgacgagggc
     2401 aaggaatacg tgggcctgca gcgcctgcgg ggccgcctct tcgtagacgg caaggtcaac
     2461 gacacgacca acgtcgtgat gcagctcggg acgggtgggt tcaatcctcg atcgcaggac
     2521 gccaccttga gcggcgacgg cagttccaag gaagtgacgc tgcgccaggc atacgtgaac
     2581 tggcggccga tggccgatct cgcgctgacg gcgggcaaga tgccggtgcc gtgggtcgtc
     2641 aacccggtcg actacttcca cgacaccgat tacttcccgg aaggcatcgc ggtgaagtac
     2701 ggcgccgcca ccggtttcca cgcctccggg ttctggctgc agatcgccga gcgcggcggc
     2761 gacgacgaaa cgacgatgct cggaacacag ctcggctatg cgagcgacct gttcttcgcg
     2821 aacgtcgcct accaggactt ccagaagatc gagggctaca acccctgcta caacggcaac
     2881 tgcaacggca acaccgtcga cctgggcggc aacctcgtca acgacttcaa cctgctgcag
     2941 gtccgcggcg gcgtgacgct cgcgggtgtc agcctcttcg cgtcctgggc gcagaacctc
     3001 gaggccgacg acgaggacac cgcgtacagc gtcggattca actacggaaa ggtgaaggac
     3061 ccgggctcgt ggtcggccgg tgccctgtac caggacgtcg aaaaggatgc gctctacggc
     3121 ggaatgttcg acggcacgtt cgcgggcggc cgcacggcgc acgacggtta cgtgctgagt
     3181 ggcgcgtacg ggttctcgaa gaactggtcc ggcagcctgc tctggttcat caatgaggtg
     3241 gacaagaccg gcaacccgca cgactacgac cggtaccagt tcgacctgct ctggaaattc
     3301 tgagtccgca ggccgcaagc cgtgcaggat ttcggcgact ccttcgggct cgctttccag
     3361 ctggtcgccg ccggcgactc gcagctgctg gaaatcatcc tgctttccct gcgcgtgagc
     3421 ctgacggccg tcgcgatcgc ctgcctgatc ggtatgccgc tgggcgcggc gctggccgtc
     3481 agccgctttc cgggccggcc tgcggtgctc gtgctggtca acgcgctgat gggccagccg
     3541 cccgtggtcg tcggcctcgt catctacctg ctgctgtcca acgccgggcc gctcggctgg
     3601 ctgcaactgc tctacacgcc gaccgcgatg atcatcgcgc agatcgtgct gatcacgccg
     3661 atcgtggcgg ctctgtcgcg cgaggtcgtc gagcagttgt acgtcgagtg cgccgagcac
     3721 ttccgctcgc tgtgcctgac ccgtatccag gcggttcgtg cactgctgtg ggacgcgcgt
     3781 ttcagcctga tcaccgtcgg gctggcgggc ttcggccgtg cgatcgcgga ggtgggcgcc
     3841 gtgatcatcg tgggcggcaa catcgcgcac ctcacccgcg tcatgacgac ggcgatcgcg
     3901 ctcgagacct cgaagggcga cctcgcgctg gcattggcgc tcgggctcgt gctgctgacc
     3961 ctcgcgctgt ccgtaaacgc ggcggtgatg atgctgcgga tgatggacga cggccatggc
     4021 caccattgat cctccgttcg tgacggcggc gggcacggtg ccggcgagcc cgcccggcat
     4081 ttccggggcg gcgcggcagc tgtcgtatcg cgtcggcgac gccaaccgtg tcgacgacgt
     4141 cgacctcgac ctctcaccga gttcgttgac ggtcgtgatg ggccccaacg gcgcgggcaa
     4201 gagcctgctg ttgcgcctgc tgcacgggct gatcgagccg acgggcggtg gagtctactg
     4261 gggcggacgg ccgctggacg aatcactgcg ccgacggcag gccatggtgt tccagcgacc
     4321 cgtgctgctg cgccgatcgg tggcggccaa cgtccggttc gtgatgcggt tgcgcggact
     4381 cgactcggtc gaacgagtgc gggatgcgct ggacgaggtc gggctggcgg accgggcccg
     4441 acagccagca cggttgctgt cggggggaga gcagcaacgc ctcgcgttgg cgcgtgcgct
     4501 cgcgatcgag ccccaggtgc tgttcctgga cgagcccacg gcgaacctcg atccgttctc
     4561 gatggccgcg atcgagtcga tcatcgcgcg cgttcacgaa cgcggcacca agatcgtcct
     4621 cgtcacgcac gacctcgggc aggcccgccg cctggccgac gacgtgatct tcctgaaccg
     4681 gggcaggctc atcgagcact cgcccgccga gcggttcttc gagcatccga gttccacgca
     4741 agcccgcgat tacctggcgg gacgactcgt cctgacgtag cgcaagccga tcgcaaggag
     4801 aaccctcgtg tccgagagac tcatcaccgg cttgctgtcg atggcgctgc tggccgcgac
     4861 ggctaccccg atgtcgaccc tggcacagga aacgtcgatc ctcgtgcagt ccaccacctc
     4921 gacccagaac acgggcctgt acgaatacct gttgccgaag ttcacggcca agacgggcat
     4981 cggcgtcaac gtcgtcgcgg tgggcaccgg acaggcgatc aagaacgcga tgaacggcga
     5041 cggcgacgtg ctgctggtgc acgacaagcc ggccgaggaa aaattcgtcg ccgagggcta
     5101 cggcgtcgag cgcttcgacc tgatgtacaa cgacttcgtc gtcgtcggtc cggccaacga
     5161 ccccgcgggc atcgcgggaa tgaaggacac cgaggccgcg ctgaagaagc tcgcggggaa
     5221 gaagagcctt ttcgcgtcgc gcggcgacaa ttccggcacg cacagcaagg aactggcgct
     5281 gtggaaggcg gcgggcatcg acccggccgc ggccagcggc acctggtacc gcgagaccgg
     5341 ttcgggcatg ggcgccacgc tcaacacggc cgtgggcatg ggtgcctacg cgctgaccga
     5401 ccgcggcacc tggatcagct tcaagaacaa gggcgactac aggatcgtca tcgaaggcga
     5461 cccggacctc ttcaaccagt acggcgtgat cctggtcaat cccgcgaggt ttccgaacgt
     5521 gaaggcaaag ccgggccagg cgttcgtcga ctggctgatc agcgacgagg gccaggcggc
     5581 catcgcgggc tacaggatcg agggccaggc gttgttcttt ccgaacgcga ggaagtagcg
     5641 caagcccgct cagtcggtcg gggagctccg aaacggcgcg cgagcgatcc cgcggtcggt
     5701 caggccttca agggattgaa tgcaggaatg cggaacttgc cggtgtcgag cgtgatccgc
     5761 tcggctgcgt agcggtccac gagcaggcgc gtgagccagg cgtgggcttc gtccagcgcc
     5821 gtgagatcga cctggcgcat gccctccgcg agcgcgagcg ggtcgtcgtt ctcggcgtac
     5881 gaacgtcgca actgctggaa ctggctgcac agttcgagga tgcgcacggc ctgctgggcg
     5941 tcgtagcccg agagttcggt gcgacgtgac attcgtgagg gcccttggtt tcgttgttcg
     6001 gcagctgcac gggcggcgaa aaggctgcaa gttctgcgcc atcctggtgc aacgctcgta
     6061 ccgacaacga cttgcgcttt ggaatcgatc cgagtccccg tgccgggccg cgcagtgtaa
     6121 agatcaccga cgcccgggtg cgcggcgacc gaacgtcgcg tccgccgacc gacccttcct
     6181 gcaacggctc ccgacgtgct gaccttccgg ccggtccagc gtggcggtct aatgcaagca
     6241 acagatcacg tatcctcggt acccggagtg ccccagaccc gttcccaaac cgacgccagc
     6301 cgcacgcggc cgcacctgcc gatgcagggc atcgcctggg cgggcctcct gctcacgctg
     6361 atcttcacgc tcgggtactg gcgctacacc gacctcgacc tcgaccgccg cgtcgaccag
     6421 cacttccggc agctcgcgca acgccaggtc gacgcgctcg tcgagcgcat gcacgcgcac
     6481 gagcgtgtcc tgcgcggcgc gagcgccttg ttcgcggcga gcacggccgt cagtcgcgag
     6541 gagtggcgtg cctacatcga caatctcgac ctcgagctga cgctgcccgg cgcccagggc
     6601 acgggcttcg ccgcggtggt gccgcgcgac gagcgcgagg ctcacgagca ggcggtgcgc
     6661 gcggagggct tcgcggacta cgcgatcgag cctgcgggcg accgaccggt ctactcgagc
     6721 atcgtctacc tcgagccgtt cgacgaccgc aacctgcggg cgttcggcta cgacatgtac
     6781 tccgagccga cgcgccgcgt ggcgatggac cgggcccgcg acaccggcgg gccggcgatg
     6841 acgcacaaag tcacgctcgt ccaggaaacg gacgaggacg ttcaggccgg cttcctgatc
     6901 tacgtgccgg tctacgccgc caaccagccc ctgcgctcgg tcgaggagcg acgcgccgcg
     6961 ctggtcggat gggtgtacag cccgtaccgc gcggcggacc tgatgaggtc ggtgttccag
     7021 ggcttcaacg gcgacgccga agtccaggtc ttcgacggcg agccggagcc ccacaacctg
     7081 ctgtacgcga cgcgcgacgc ggcgcgtgca gccgagcacg aggccacgtt caaggtcgaa
     7141 atcggcggca taccgtggac cgcgagattc cgcagcagcg cggcgttcga cgccggcatc
     7201 gaccgcctgc agtccaggct ggtgctgctg gccggcgtgc tgctgagcat gctcgtgttc
     7261 tcgttgctgt acgccgaaac gcggcatcgc gcggggctcg aggcgcaggt gcgcgagcgc
     7321 acgctcgaac tcgaaaaagc gcgcgacgag gcggagggcg ccagtcgcgc gaagagcgcc
     7381 tttctcgcca cggtgagcca cgagctgcgc acgcccctga acgcgatcat cggcttcagt
     7441 tcgatcctgc tgcaggacag cctgagctcg gagcagcgca agcagctcgg catcatcaac
     7501 cgctcggggc tgcagctgct cgacctgatc aaggagattc tcgacatcac cagcatcgag
     7561 gccggccacc tggcgatgca cctcgagccc gtgaacctgc gacagctgct cgaggagcag
     7621 tgtgaagtgc tgcagatgca ggcccgcgac aacgggctct acctcaagct cgcggagtgc
     7681 gaaccgccgg tgatcgtggt cgcggaccag ggacggctga gccaggtcgt gcgcaacctg
     7741 gtgtccaacg ccatcaagtt cacggatcga gggggcgtga cggtgcgctg ccgcaccgtc
     7801 ggcgacacgg cgatggtcga ggtcgaggac accggcatcg gcataccgca ggaggcgcac
     7861 gcgacgctgt tcgtgccgtt ccagaggggc gaccggcacg gtggccgccc cggcaccggc
     7921 ctcgggctcg cgatcagtcg ccggctggtt gaggcgatgg gcggccagat cggcttcgag
     7981 agcgtcgtcg gccagggcag ccgcttctgg ttcaccgtgc cgcgttcgcg gaccgagcgc
     8041 cgcgcggcct gatcgttcag acctcgccgc ggcgcgcgaa tcgcgcgatg tgctcgccgg
     8101 cacggaacgc gagcgccatg atcgtcatcg tcggctgccc acgaccggac gtcacgaagc
     8161 tcgagccgtc acagacgaac aggttgcgga cgtcgtgagc gcggtgaaag cgatctacga
     8221 ccgacgtgcg cgggtcgtcg cccatgcgcg ccgtgccgag caggtgcggg ctgcccacgg
     8281 cctcggtgac ctggtccttc cagacgtcga gcgcgccggc ggcctgcatg atctcgacgc
     8341 cgcggtcctg caggaaacgc gcaaacgcca ggtcgtccgg gtggtcgcgg taggtcatcc
     8401 gcagcgccgg caggccccag gcgtccttca gtgccgggtc gaggtcgatg cgattcgagg
     8461 cgagcggcag cgaggtaccg tgcgtcgcca cgaccatcgc acggggcatt gcttcgagca
     8521 gcgacttgag cgcggcgccc cagcggcggc cgggcggcgg cgcaaacgca ccccagacga
     8581 tcggcatcgg cccgctgcgc gcgtcgagcc cgccgccgcc gtagcagccg cgcgccgggt
     8641 cggtctcgta gtagtcgtgc agcacgcgcg tgacctggac gctcttgaac tcgttcagct
     8701 cgtgctcgaa cagtgcgtgc acctgcgcat aagtgttgaa catcaggttg cggccgacca
     8761 tgccgctcga gttggcgagg ccgtctggat gcctcgcgtt cgccgacagc agcagcagcc
     8821 gcgcggactc ggcgccattc gcgcagagca cgaccgcgcg cgccttctgg aagtgctcgc
     8881 ggcgggccgc gtcgaagtac gcgacgccgg tgacacggtc cttgggaccg gtctcgatcc
     8941 gcgtgacgta gctgtgcggt cggatttcgc agcgcccagt cgcctccgcc tccggcagca
     9001 tcgtgtacag cgtcgacgac ttcgcgcgca tctcgcagcc gtagcccatg cagaacccgc
     9061 agtgctggca ggccggccga tcgcgaaacg gctcggaggc gatcgccatc ggcgccggga
     9121 acggatgcag cccgagcttg cgtgcgccgc gttcgaacag cacgcccgac gacttcaccg
     9181 gcagcggcgg catcggatac ggcttgctgc gcggcggatc gaacgggctc gcgccggcga
     9241 ggccggacac gccgacctcc cactccaccc gggtgtagta cggctcgagc tccgcatagg
     9301 tgatcggcca gtcgtcgaac gtcgtgccgg cgatcggtcc gagccggctg cgctcgtcga
     9361 agtccacttc gtggaagcgc cagaagttgc ccgtgaagtg cgtgctgctg ccgcccaccg
     9421 tcggggcgta ccacgcacac gggaaatcgc gcacggcgac ggcggtctcc gcctccgacc
     9481 gacgaaagct ctgcgggttg gtcgagagtt tgttgatgag ccccgagttg agccacacgt
     9541 cgaactcgtc gtgcgtgaag tcggcggcgg tcagccgtga tccctgctcg agcacgagca
     9601 ccgtgctgcc cgcccgcgcg agttcccgcg ccatgacgcc gccggccgcg ccggacccca
     9661 cgacgacgaa gtcgacggtt tcgcgtgggc cgaatcgacg tgcgctcatg tgcgcttgcc
     9721 cggcgtgccg gagtacggcg tgaagccctg gtagcccgcg tcgtaatgcc cgaacggcgg
     9781 ctgccaggcg tgctggtcca cgaaccccac gagcttccag cccgccttct gcgcgttgcc
     9841 gccgtagcgg ggcgaggcca gcaggcccac gaccgtcagg aagcgcacct gctcgaagaa
     9901 cggcgtgcgc tcgacgtgct gcaggtacgc aagctggtct gcggcactca ggtcggcgaa
     9961 ctgcgccgtg ccggggtggt ggcgcgcaca gcgggcctgg aagtcgcgca ggccttcgag
    10021 gaattgcgtc gcaccgcccg accaggggcc cgcgtgcacc tggtcgatga aatacacgac
    10081 cgccgcctcg cgtgcgccgg gcgtgtcgcc accgggcacg atctgcgccg cgatcgcctc
    10141 gacgtcgcgc gcctgccggg catcgaacag ccggaaccgg gagcggtccc cgccgtccgc
    10201 ggcgctggcc gcgtgcgcgt gctcggcggc cgcgacgatc tgcggccagt tggccgcgag
    10261 ccacgcgaat ccgagcgacc cgccggatga ctgaaggaac cgacgccgcg agagttcgtt
    10321 cgagtccatg gcgcgcctcc ccgaaggcaa cggaaccgtg acaccggccc gcgcgcggca
    10381 gtatgggccg acggtgcgct gctggcgagg acgcgcgcgt tcgcctatct tcgggacgac
    10441 atcccaggga gtcgtcatgt cgcagcccaa ccccgtccgc tggttcgaga tctacgtgca
    10501 ggacatggcg cgcgcgaggc gcttctacga gacagtactc gacaccacgc tgacgcgact
    10561 cgactctggc ggcccggaga tgtgggcctt tccctcgacg cagggcgagt acggcgtcgg
    10621 cggcacgctc gtgaagatcg acggcgtccg gtcggggggc aacagtacgc tggtctactt
    10681 cggctgcgac gagtgctcgg tggaagccgg ccggatcgtg ggcgccggtg gcacggtgca
    10741 gcgcgagagg atgtcgatcg gcccgtacgg ttacgtcgtg ctcgcgctcg ataccgaggg
    10801 caacatgatc ggactgcact cgacgcggtg aaccaaccgg gcgcagtttg cgtccaagcg
    10861 tcacaggccg cgctcgagga gggacgcccg tgacgaggca atcgcagacc ttcaccatcg
    10921 caacgatcgt cggcgtgtcc gcgatcatgc tggtcgcgtg tgggcgggcg ccgaccggtt
    10981 ccgtcgatgc cgcgggcgtc tcgcccgcgc cggaggtcgc gacgctcgac gcggaccagc
    11041 caacgggcgc ggcgcgcgtc gtgaccgcgg ctgggacggc gccgcagccg ggcgacgagt
    11101 tgcgccggcg cgaagccgaa ctcgccgcgc gtgagcgcga actgcgcgtg cagcaggacg
    11161 aactgcgccg cgagcgtgag cggctcgagg ccgcccgtgg cggcggtcgc ccctccacct
    11221 ctgcaagcga cgactccttc gacgcggacc aggcgacggc gcccgcggcg accacgcccg
    11281 cgcacagcag catccagaag gtgccgacgc cgccgatcgt cgtgccgcca ggcacgccgc
    11341 tcaagatcga actcgccgcc aacgtggaca cccgggtcgc gcgcgtgggc gaccgcgtgg
    11401 agggccgcct ggcgtccgac ctcgtggtgg gcgagcgtcg tgccgccgtg gccggtgccg
    11461 ccgtgacggg cagagtgacc gagctcgtcc ctggccgcga cgaggccggc ggcctgccga
    11521 cgctcgtcgt gacgttcgag tcgctgcagg ccgccaacgg cgcgacggtt cccatcgtcg
    11581 cgaagttccg ccagcaggac gacagcgata tggcgcgcga ggctgccgag ggggccggcg
    11641 gtgccgcctt cgtggcagcg gcgcagagaa ccggcggcga agtcaggatc cgcgccggca
    11701 cggtgatcac cgcgccgacg gagacctcgc tgtcgatcta ctgacccgaa ccgctacaac
    11761 ttgccgtcgt aagggatcgc gtcgaccggc aggtcgaagg cgatgaacag cgtgcaggga
    11821 tcctggctgc gacacttcgc agagtgcggc gcttcggccg gcccgtacgc gtattgaccg
    11881 gtctgcagca cgatcggcga gcggccctgg tacgtcacct cgagttcacc cccgaccagc
    11941 accatgcgtt ccgccgaagt gtgcgagtgc ggcgcaatgg cgtagccgcc gggaacctgg
    12001 aagaacaggt cgacgccggg cttcgccggg tctccatgca ggacggcaat acggcagcct
    12061 tccgcgaaca gcggcggaca gggcgcccat ttgaggttcg gatcgttcac cttgatcgcg
    12121 agcggtgtgc gagccggatc cggcacgacg gcttccgcct tggcctcggc cgtcaccttg
    12181 ctcgtcgcgg ccggcgacgt agtgccgggc agcgtctgcg gcgccgtcgg cgcctgggtt
    12241 tccgcttgct gcgcgtgcgc aagcgacagg gcgctcgcgg cggcaatggc gagcgtcgcg
    12301 gcggcgtaga tccaggcggt gcgtttcatg ggtcgtgtca cgcgtgattc cgtgggcgac
    12361 gcgcccttga tatggcgaag cgcggcgtcc gtctgtagga aagctggcaa acttcgtgca
    12421 tgaccgatcg gatctccgac ccgtggcggc tgtcgcgctt cgtcgaggcg cagtcgggcg
    12481 tgtacgagcg cgcactcgcc gagctgcgcg acggccgcaa gcgctcgcac tggatgtggt
    12541 tcgtcttccc gcagctggcc gggctcggct tcagtgcgat gtcggtgagg tatgcagttt
    12601 cgggtctcgc cgaagcgcgc gcgtacctcg atcatccggt gctcgggcca aggctgcgcg
    12661 agtgcgcggc cgcactgctc gcgctcgacg agcgctgctc cgccgccgcg gtcatggaat
    12721 acccggacga tctgaagctg cgatcttcgg ccacgctgtt cgcgcaggtc tcgtcgccgg
    12781 ggtcggtgtt tcaccaactc atcgaccgtt acttcggcgg caagcccgac gaggtgaccc
    12841 tgaagctcgc cacggcgggc gacgcgtgag gtcgagcatt ttcgcaggca tcgcgatagc
    12901 ggccgcaggc ctcctcgcgt gcactgcatc gcaggcttcg acgccgcgcg ccggccagcg
    12961 gtcgccaccg gccgaagtgg cgcaggcgct ggccacgccc ggcagcagcc cggtgctgcg
    13021 gggcagggtg atcgacgtca tcgacggcga cagcgtcgcg gtgcagctgc agaccggacg
    13081 catcgaagtg cggctgcacg cggcggacgc acccgaacac gaccagcccg gcggtcgcgc
    13141 ggccggccgc gcgctgcgca agcgcctgcc gcgcggcagc gacgtcctcc tcgagccgtt
    13201 cgaacaggac cagtacgacc ggctcgtcgc cgtgatcgag caggacggcg aaagcgtgaa
    13261 cgcctggatg gtggagcaag gccatgcctg ggcgtaccgg cagtacacca ccgatgcgcg
    13321 ttattgccgc tgggaggacg cggcgcgggc agcgcaccgc ggtctctggt cgagcccgcc
    13381 cggggagtgg atcgcaccgt gggactggcg tcgtcgcgcg cgcgaacccg gctatcgacc
    13441 ggcggaccgc tcgcgcgaga cgctcgcgga ttgccgggcg tcgctgcggc accgccgcaa
    13501 cgccgctacc ggtgcgcgca cgcctcgctg acgatgcccg cgcctgagtt ttcctaccgc
    13561 gagccgggcg cccgtcgcct gagcagcgtg cgggtgcgtt gctatggtgc ggatcgcatg
    13621 gaagcgaatt cgaggaggat gccatggccc aggaccagcg cggcagatcg gaacggaagg
    13681 gcagcggcga ccaggtgagc ggcggccagg agccgcgcac cttgccggcc ggcgtgacgt
    13741 ggcacggcga gggcccgcac gcggacgaag agcagc
//
LOCUS       DASPFR010000269         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_564268, whole genome shotgun sequence.
ACCESSION   DASPFR010000269 DASPFR010000000
VERSION     DASPFR010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564268"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="VD737_07215"
     CDS             <1..1018
                     /locus_tag="VD737_07215"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HXW10386.1"
                     /translation="PDRPETGYGYICRKNGDTPEFPGAEIRNVPIYPVERFVEKPDEA
                     TAKQYVESGQYYWNSGMFMFRATAYLAELERHAPAMLSACQDAVAAAVRDLDFTRLPK
                     DEFAACPSDSIDYAVMEKTSLAVVVPLDAGWSDVGSWSALQDALPRDGHGNVTTGDVL
                     AEDSSGCYLHSTSRLIGAVGLKDHVVVETKDAVLVAPRDRVQDVKQLVATLKKQGRYE
                     TALHREVFRPWGSYDSIDTGERFQVKRLIVKPGATMSLQMHHHRAEHWIVVSGTARIT
                     RGEETFLLGENESTYIPLGTKHRIENPGKVTLEIIEVQSGSYLGEDDIVRFEDIYGRQ
                     GTNK"
     gene            1061..>2259
                     /locus_tag="VD737_07220"
     CDS             1061..>2259
                     /locus_tag="VD737_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HXW10387.1"
                     /translation="MNPIKIQGFKAYDLRGRIPGELNEDVAYRVGRAYAEFVKPRKVV
                     VGRDIRLSSEQIGKALEQGLLDSGVDVYDVGLCGTEVVYYATFAREMDGGVMVTASHN
                     PPDYNGMKFVREGSRPISGDTGLVEIRELAEAGRFTKPTRVGERFAVDIMPDYIEHLL
                     SYVDRAKLKPLKVVVHSGNGGAGLIVDKLEPYLPFEFIKVQHEPDGHFPNGVPNPMLA
                     ENHVAPVEALRKHGADCAISWDGDYDRCFLFDEQGNFIEGYYIVGLLAAAFLQRNPGA
                     RIVHDPRLTWNTLDIVQRMGGEAVQSKSGHAFIKAKMREVDGVYGGEMSAHHYFRDFS
                     YCDSGMIPWLLVTQLMCTTGKPLSELVGERIRLFPASGEINRRVDDAKSVIANVLAQY
                     QPIAKDI"
BASE COUNT          423 a          747 c          748 g          341 t
ORIGIN      
        1 gcccgaccgg cccgagaccg gctacggcta catctgccgg aaaaatgggg acactcctga
       61 atttccgggt gccgaaataa ggaatgtccc catttatccg gtggagcggt tcgtcgagaa
      121 gccggacgaa gccaccgcga agcagtacgt cgaatccggc cagtattact ggaacagcgg
      181 catgttcatg ttccgcgcga cggcctacct cgccgagctc gagcgtcacg cgccggccat
      241 gctctcggcc tgccaggacg cggtcgccgc cgcggtgcgc gacctcgact tcacgcgcct
      301 gccgaaggac gaattcgccg cctgcccgag cgactcgatc gactacgcgg tcatggaaaa
      361 gaccagcctc gcggtcgtcg tcccgctcga tgccggctgg agcgacgtgg gctcgtggtc
      421 ggcgctgcag gacgcactgc cgcgcgacgg tcacggcaac gtcacgaccg gcgacgtgct
      481 ggccgaggat tccagcggtt gctacctgca ttccacgagc cggctgatcg gcgccgtcgg
      541 gctcaaggac cacgtcgtcg tggagaccaa ggacgccgtg ctggtggcgc cgcgcgaccg
      601 cgtgcaggac gtcaagcagc tcgtggccac gctcaagaag cagggccgct acgagacggc
      661 gctgcaccgc gaggtgttcc gcccctgggg cagctacgac agcatcgaca ccggcgagcg
      721 cttccaggtg aagcggctga tcgtgaagcc cggcgcgacg atgtcgctgc agatgcacca
      781 tcaccgcgcg gaacactgga tcgtcgtctc gggaacggcg cgcatcaccc gcggggagga
      841 gacttttctg ctaggcgaga acgaatcgac ctacatcccg ctcgggacga agcaccgcat
      901 cgagaacccg ggtaaagtca cgctcgagat catcgaggtg cagtccggca gctatctcgg
      961 cgaggacgac atcgtccgct tcgaggacat ctacggccgg caaggaacga acaagtaaac
     1021 gccgtctgcc gctgcgcggg agagggccgg gcgagggcgc atgaatccga tcaagatcca
     1081 gggcttcaag gcctacgacc tgcgcgggcg gatcccgggc gagctcaacg aggacgtcgc
     1141 ctaccgggtc ggtcgcgcgt acgcggagtt cgtgaagccg cgcaaggtcg tcgtcggccg
     1201 cgacatccgt ctctcgagcg aacagatcgg caaggccctc gagcagggcc tgctcgactc
     1261 cggcgtcgac gtgtacgacg tcggcctctg tggcaccgaa gtcgtctatt acgcgacgtt
     1321 cgcgcgcgaa atggacggcg gcgtcatggt caccgcgagc cacaacccgc cggactacaa
     1381 cggcatgaag ttcgtgcgcg agggctcgcg cccgatcagc ggcgacacgg ggctcgtcga
     1441 gatccgtgaa ctcgccgaag ccggccgctt cacgaagccg acgcgcgtgg gcgagcgctt
     1501 cgccgtcgac atcatgccgg attacatcga gcacctgctg tcgtacgtcg accgcgcgaa
     1561 gctcaagccg ctcaaggtcg tcgtgcactc gggcaacggc ggcgccgggc tcatcgtcga
     1621 caagctcgag ccgtacctgc ctttcgagtt catcaaggtg cagcacgagc cggacggcca
     1681 cttcccgaac ggcgtgccga acccgatgct cgccgagaac cacgtcgccc ccgtcgaggc
     1741 gctgcgcaag cacggcgcgg actgcgcgat cagctgggac ggcgactacg accgctgctt
     1801 cctgttcgac gagcagggca atttcatcga gggttattac atcgtcggac tgctggcggc
     1861 cgcgttcctg cagcgcaatc ccggcgcgcg gatcgtccac gacccgcgct tgacgtggaa
     1921 cacgctcgac atcgtacagc gcatgggtgg cgaggccgtg cagtcgaagt cagggcacgc
     1981 gttcatcaag gcgaagatgc gcgaggtcga cggtgtctat ggcggcgaaa tgagcgcgca
     2041 ccactacttc cgcgacttct cgtactgcga cagcggcatg atcccgtggc tgctcgtgac
     2101 gcagctgatg tgcacgacgg gcaagccgct ctccgaactc gtcggcgagc gcatccggct
     2161 gtttccggcg agcggcgaga tcaaccggcg cgtcgacgac gcgaaatcgg tcatcgccaa
     2221 cgtgctggcg cagtaccagc cgatcgccaa ggacatcga
//
LOCUS       DASPFR010000270         3433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_197962, whole genome shotgun sequence.
ACCESSION   DASPFR010000270 DASPFR010000000
VERSION     DASPFR010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197962"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..649
                     /locus_tag="VD737_07225"
     CDS             248..649
                     /locus_tag="VD737_07225"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXW10388.1"
                     /translation="MNPPALVTHRGGCHCGAVAFEVDAPAHLVVQDCNCSICSSSGFW
                     HLIVPRERFRLLRGADSLTEYTFGTGVARHLFCRTCGVKSFYVPRSHPDGYSVNARCL
                     DRATVATITLEPFDGRNWEASVGSLRRLDES"
     gene            complement(668..907)
                     /locus_tag="VD737_07230"
     CDS             complement(668..907)
                     /locus_tag="VD737_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10389.1"
                     /translation="MKFTLLFALLLVVAVLVTINARKRRVREAQAAAVQARQRAARRN
                     RVPDVSSNLKGVTASQTLSPYRPGAPREPGDERVA"
     gene            complement(1103..3100)
                     /locus_tag="VD737_07235"
     CDS             complement(1103..3100)
                     /locus_tag="VD737_07235"
                     /inference="COORDINATES: protein motif:HMM:NF005539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXW10390.1"
                     /translation="MTYFVTGATGFIGRFLVQALLERGKGTVYVLVRPKSLGKLDELR
                     QFWGKDSTRRVVPIKGDLGQSKLGVAKADLTRLKGKVKHFYHLGAVYDLEASAEEMHE
                     ANIVGTEGALAVASAIGAGCFHLVSSIAAAGLYRGTFTEDMFEEAEELDHPYHRTKHD
                     SEALVRANGKVPFRVYRPGIVLGHSQTGVIDKIDGPYYFFKALQKLRESWPRWVPLVG
                     IEGGYVNAVPVDFVVDALVHLSHLPKLDGECFHLTDPKTRRMGEVINVFARAAHAPEM
                     AFRVDPKVFQMLPEPVTHSVERSKPIQNVFNQLLRDLQVPRSVLQFVNYPTRFDSTAT
                     QRLLDKAKIRVPPLEDYAWRLWDYWERHLDPDLSIDRSLKGAVKGKVVVITGGSSGIG
                     EAAALRIAEAGGKVLVVARDEDKLAEVRKKIVDAGGFARTYSCDITDYDANDRLAKEI
                     LKDFGHVDVLVNNAGRSIRRSLALSYDRFHDFERTMQLNYFACVRLTMNLLPSMTARK
                     AGHVINVSSIGVLTNAPRFSAYVASKAALESFARCAASEFHDEGVHFTIINMPLVRTP
                     MIAPTKIYEDMPTIISPEEAADMIADAIIRKPQRIATRLGLMAEIMHLVMPKLSEVVM
                     NSAYRMFPDSAAAQGRREGEEPRPPTREAMIFASLMRGIHW"
BASE COUNT          549 a         1118 c         1172 g          594 t
ORIGIN      
        1 gtcggtcgat ctcagtttcg gcctgaaaat cgacgcgagc cgaacgaatt cgaacacgcg
       61 tcacggacct gggcggctcg ccggcccaca ctgccggtca tcccctcacc gatgactgga
      121 gaacccgtgg aaatcgccgc cgtggtcttg ttcggatccg tcgcactctg gctgacggag
      181 tctttgacgg cccgctgacg cagcagctga ggcggccgcg atggggcatg gcgtatggta
      241 agcccccatg aatccgcctg ccctcgtcac gcatcgcggt ggctgccact gcggcgccgt
      301 cgcgttcgag gtcgacgcgc ccgcgcacct cgtcgtgcag gactgcaact gctcgatctg
      361 ctcctcgtcg gggttctggc acctgatcgt gccacgcgag cgctttcgcc tgctgcgggg
      421 agccgattca ctcaccgagt acacgttcgg gacgggggtg gcacgacacc tgttctgccg
      481 tacctgcggc gtgaagtcgt tctacgtgcc gcgctcgcat cccgacggct acagcgtcaa
      541 tgcgcgctgc ctcgaccgcg cgaccgtcgc gacgatcacc ctcgagccgt tcgacggccg
      601 gaactgggag gcgtcggtcg gatcgctgcg ccggctggac gagtcgtgag cccgcgcgcc
      661 tgagtgatca ggcgacgcgt tcgtcacccg gttcgcgggg tgcgccgggc cggtacggtg
      721 acagggtctg gctcgccgtg acgcccttga ggttgctcga gacgtcgggc acgcggttgc
      781 gccgcgcggc ccgctggcgg gcctgtaccg ccgccgcctg ggcttcacgc acgcggcgct
      841 tgcgggcatt gatggtcacg agcacggcga cgaccagcag cagcgcgaac aggagcgtga
      901 acttcatgga tcccttcctt gaaccctggc aggacagccc accgggcgcg gttctgccac
      961 acagcgaccg gcgctgcctg ggcgcaagta tccgcgcagg ggacgccgcg aaacagctga
     1021 ctacggccca gattgaacca gattggcagg tgcggcctgc cgccggcgct actgcgcgct
     1081 gcgtcacggt tctgccgggg gattaccagt ggatgccgcg catcagcgaa gcaaagatca
     1141 tcgcctcgcg cgtcggtggt cgcggctcct cgccttcccg cctgccctgc gccgccgccg
     1201 aatcggggaa catccggtac gcggagttca tgacgacctc cgagagcttc ggcatgacga
     1261 ggtgcatgat ctcggccatc aggccgaggc gcgtggcgat gcgctgcggc ttgcggatga
     1321 tcgcgtcggc gatcatgtcg gccgcttcct ccggcgaaat gatcgtcggc atgtcctcgt
     1381 agatcttggt gggcgcgatc atcggcgtgc gcacgagcgg catgttgatg atcgtgaagt
     1441 gcacgccctc gtcgtggaac tccgaggctg cgcagcgcgc aaacgattcg agcgccgctt
     1501 tcgacgcgac gtacgcggag aagcgcggcg cgttggtcag cacgccgatc gaggacacgt
     1561 tgatgacgtg gccggccttg cgcgccgtca tcgacggcag caggttcatc gtcagccgca
     1621 cgcaggcgaa gtaattgagc tgcatcgtgc gctcgaaatc gtggaaccgg tcgtacgaca
     1681 gcgccagcga acgccggatc gagcgtcccg cgttgttgac cagcacgtcg acgtgcccga
     1741 agtccttgag gatctccttc gcgagcctgt cgttcgcgtc gtagtcggtg atgtcgcagg
     1801 agtaggttct ggcgaaaccg ccggcatcga cgatcttctt gcggacctcg gcgagcttgt
     1861 cctcgtcgcg cgccacgacc agcactttgc cgccggcttc cgcgatgcgc agcgccgccg
     1921 cctcgccgat acccgacgag cccccggtga tcacgacgac cttgcccttg accgcgccct
     1981 tgaggctgcg gtcgatcgac aggtctggat cgaggtgccg ctcccagtag tcccagagcc
     2041 gccaggcata gtcctcgagt ggcggcacgc ggatcttggc cttgtcgagc agccgctgcg
     2101 tcgcggtcga gtcgaagcgc gtgggatagt tgacgaactg cagcaccgaa cgcggcacct
     2161 gcaggtcgcg cagcagctgg ttgaagacgt tctggatcgg cttgctgcgt tcgaccgagt
     2221 gcgtgaccgg ctccggcagc atctggaaga ccttgggatc gacgcgaaac gccatctccg
     2281 gcgcgtgggc ggcgcgcgcg aacacgttga tgacttcgcc catgcgtcgc gtcttgggat
     2341 cggtgaggtg gaagcactcc ccgtccagct tcggcaggtg cgagaggtgc accagcgcat
     2401 cgacgacgaa gtcgacgggc acggcgttca cgtagccgcc ttcgatgccg acgagcggaa
     2461 cccagcgcgg ccacgattcg cgcagcttct gcaatgcctt gaagaagtaa tacggcccgt
     2521 cgatcttgtc gatcacgccg gtctgcgagt gaccgagcac gatgccgggg cgatagacgc
     2581 ggaacggcac cttcccgttg gcgcgcacga gcgcttcgga gtcgtgcttg gtacggtggt
     2641 aagggtgatc gagctcctcg gcctcctcga acatgtcctc ggtgaacgtg ccgcggtaga
     2701 ggcccgccgc ggcgatcgaa ctgacgaggt ggaagcatcc cgcgccgatg gcggaggcga
     2761 cggcgagcgc cccttcggtg ccgacgatgt tcgcctcatg catctcctcg gccgacgcct
     2821 cgaggtcata gaccgcaccg aggtgataga agtgcttgac cttgcccttg agtcgcgtga
     2881 ggtctgcctt ggcgacgccg agcttcgatt gcccgaggtc gcccttgatc ggcacgacgc
     2941 gccgcgtgct gtccttgccc cagaactgcc ggagctcgtc gagcttcccg agcgacttcg
     3001 gccgcaccag cacgtagacc gtgcccttcc cgcgctcgag gagcgcctgc acgaggaaac
     3061 ggccgatgaa gccggtcgcg ccggtcacga aatacgtcat ggagcactcc ccgggtcttt
     3121 gcaggttacg ttagggggcg tgggtagtcg cgtcaacgtg agcctcggcg gtgcaaccgt
     3181 gggcgacggt ccgtcgttac gcctcagccc cggttacgtg cgcgcgcggc gcagcggggt
     3241 gatgacgtcg gacttcggtc cggggagcgt cacgagccag ggaaggcgag ccggcagggg
     3301 cgatgccttt gcgggttgta cctaggcctc ggattgacac actgcagcaa gccttagcat
     3361 cggccagaat agtgagggcc gaggcgccgg gagttccgca atggcgaacc gcgacacgag
     3421 cagtaatcgg cag
//
LOCUS       DASPFR010000271         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1664674, whole genome shotgun sequence.
ACCESSION   DASPFR010000271 DASPFR010000000
VERSION     DASPFR010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664674"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..668)
                     /locus_tag="VD737_07240"
     CDS             complement(135..668)
                     /locus_tag="VD737_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10391.1"
                     /translation="MAERKGRDLDRDPETGQPEDHLGGVAGGAFAGGTAGGVAGAALM
                     GATTGTIAGGPIGMVSGAIAGAVAGSVIGGITGKALAERINPQHEDEYWRNEFPTRTY
                     AAGSKYGWDDYEPAFRLGYERYPDYHGRSFEDVEPELAQRWSESRGGSHLEWDDARHA
                     TRDAYERLRTEFAKRSG"
     gene            complement(813..1328)
                     /locus_tag="VD737_07245"
     CDS             complement(813..1328)
                     /locus_tag="VD737_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10392.1"
                     /translation="MSGLGRGALIALLQVALLAAVGGKLLYDRAVLPRAWIETAGVDP
                     DLPIRGRYVALSLVLPAVEEAPIPASATLAYGRIEMRDGQAVAVLAASDPVMADAAHP
                     LAFVRNVGLAGARWQTLVPVAFFLPEHARDPTLGRQPGELWIEATLPPRGAPRPVRLG
                     LRRDERIEPLD"
     gene            complement(1325..>2429)
                     /locus_tag="VD737_07250"
     CDS             complement(1325..>2429)
                     /locus_tag="VD737_07250"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HXW10393.1"
                     /translation="AGLVDEATAERIRRWETEHAAPEGSRLGRLAFGFGGLLLASGLL
                     LFVAANWQQLSPWGRFALFAVLVTALHLAAGAVQGRMPALATVLHAVGTAALGGGIFL
                     AGQAFHLSERWPEGLLLWGLGAACALWYLRDWPHVLYVASLLPAWLLAEWSASVGFEA
                     RGALAVGAVGTFVLAVAYVAAVGVDARTQWRHELSRLGAVALVFAGWALPFSAMQNEP
                     FFARGGVAMSTGWQVLGWSVAVLLPLALGWALRGREAWPLAIAVVFALVVSQLDVSTT
                     GTRALVHALYLAAAVGLTAWGLRDAHRLRVNVGTFAFALTVLSFYFSSVFDKFGRSLG
                     LIALGALFIGGGWLLERTRRRLLRRLDAPGRAG"
BASE COUNT          393 a          903 c          831 g          302 t
ORIGIN      
        1 gccgccgcct gaggaagtgg ctagtggcta gtggctggtg gctagtgact ggtgaaggtc
       61 ggccggtgtc gccaccaggt tccggtcttt cactgaccac taaccactag ccactaacca
      121 ctaattgctg accactagcc actcctcttc gcgaactcgg tccgcagccg ctcgtacgcg
      181 tcgcgcgtcg cgtggcgcgc gtcgtcccac tcgagatgcg agccgccgcg cgactcggac
      241 catcgctgcg ccagttcggg ctccacgtcc tcgaagctgc gaccgtggta gtcggggtag
      301 cgctcgtatc cgagccggaa ggccggctcg tagtcgtccc agccgtactt cgagcccgcg
      361 gcgtaggtgc gcgtcgggaa ctcgttgcgc cagtactcgt cctcgtgctg cgggttgatg
      421 cgctcggcga gcgccttgcc tgtgatgcca ccgatcaccg agccggcgac ggcgcccgcg
      481 atggcgcccg acaccatgcc gatcgggccg ccggcgatcg tgccggtcgt cgcgcccatc
      541 aacgccgcgc ctgcgacgcc acctgcggtg ccacctgcaa acgcgccgcc ggcgacgccc
      601 ccaaggtgat cttccggctg tcccgtctcg gggtctcgat cgaggtcgcg tcccttgcgt
      661 tcggccatga cgactcctta gatgctggcg gtcgggtgac tgtcgagcgt tcgtcggtcg
      721 acggaacgga gacagcgccg tgagtgcagc cttcttaagg cgctcgaccg gtcaggtatg
      781 taggagccgc cccgattcgg gcggaggcga ggtcagtcga gcggttcgat gcgctcgtca
      841 cggcgcaggc ccagccggac ggggcgcggt gcgccgcgcg gcggcagcgt ggcctcgatc
      901 cacagctcgc cgggctggcg gccgagcgtg gggtcgcgcg catgctccgg caggaaaaag
      961 gcgaccggca ccagcgtctg ccagcgcgcg ccggccaggc cgacgttgcg cacgaacgcg
     1021 agcgggtgcg cggcatccgc catcacggga tcgctcgcgg ccagcacggc gacggcctgg
     1081 ccgtcccgca tctcgatgcg tccgtacgcc agcgtggccg acgcggggat cggcgcctcc
     1141 tccacggccg gcagcaccag gctgagcgcc acgtagcggc cgcgaatggg caggtcggga
     1201 tcgacgcctg ccgtctcgat ccaggcccgc ggcagcaccg cgcgatcgta gagcagcttg
     1261 ccaccgacgg ccgcgagcaa cgccacctgc agcaatgcga tcaacgcgcc acgtccgagc
     1321 ccgctcatcc ggcgcgccct ggggcgtcga ggcggcgcag caggcgccgt cgcgtacgtt
     1381 cgagcagcca gccgccaccg atgaacagcg cgccgagcgc gatcaggccg agtgagcggc
     1441 cgaacttgtc gaacacgctg ctgaaataga acgacaggac ggtcagcgcg aacgcaaacg
     1501 taccgacgtt cacccgcagc cggtgcgcgt cgcgcagacc ccaggccgtg aggccgacgg
     1561 ctgcggccag gtacagcgcg tggaccagcg cgcgcgtccc ggtcgtggaa acgtcgagct
     1621 ggctgacgac gagcgcgaag accaccgcga tcgcgagcgg ccacgcctcg cgtccgcgca
     1681 acgcccaccc caacgccagc ggcagcagca cggccacgct ccaccccagc acctgccagc
     1741 cagtggacat tgcgacgccg ccgcgcgcga agaagggttc gttctgcatc gccgaaaacg
     1801 gcagcgccca gcctgcgaac accagcgcca ccgctccgag ccgggagagt tcgtggcgcc
     1861 actgcgtccg ggcgtcgacg cccacggcag ccacgtacgc cacggccagt acgaaggtgc
     1921 cgaccgcgcc gaccgcaagt gcgccgcgcg cctcgaagcc gacgctcgcc gaccattcgg
     1981 ccaacagcca ggccggcagc aacgacgcga catagagcac gtgcggccag tcacgcaggt
     2041 accagagcgc gcaggccgca ccgagccccc acagcagcag tccttctggc caccgctccg
     2101 agaggtgaaa cgcctgcccc gccaggaaga tgccgccgcc gagcgcggcc gtgccgaccg
     2161 cgtgcagcac ggtcgccagt gcgggcatgc ggccctgcac ggcgccggcc gcgagatgca
     2221 gggcagtcac cagcaccgca aacagggcga agcggcccca cggcgacagt tgctgccagt
     2281 tcgccgcgac gaacagcagc aggccgctcg ccagcaacag cccgccgaag ccgaacgcca
     2341 gccgcccgag gcgcgagcct tccggtgccg cgtgctccgt ctcccaccgc cggatgcgct
     2401 cggcggtggc ttcgtcgacg aggccggcg
//
LOCUS       DASPFR010000272        10280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1785107, whole genome shotgun sequence.
ACCESSION   DASPFR010000272 DASPFR010000000
VERSION     DASPFR010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10280
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785107"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /gene="rimO"
                     /locus_tag="VD737_07255"
     CDS             complement(<1..1236)
                     /gene="rimO"
                     /locus_tag="VD737_07255"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HXW10394.1"
                     /translation="MSSAPPRIGFVSLGCPKALVDSERILTRLQAEGYDVVGSYEDAN
                     LVVVNTCGFIDDAVHESLEAIGEAVARNGRVIVTGCLGARPETITDRHPSVLKVTGAH
                     AYEEVVAAVHEQLPPLHDPFVDLVPPQGIRLTPRHYAYLKISEGCNHRCTFCIIPSMR
                     GDLASRPIGEVMLEAERLVKAGVKELLVISQDTSAYGVDLKYATGFWGGKPVRARFAD
                     LVRSLGTLGAWVRLHYVYPYPHVDDVIPLMAEGKVLPYLDIPFQHGSPRVLKLMKRPA
                     HAENTLERIRRWREVCPDLTLRSTFIVGFPGETDADFELLLEWLREARLDRVGCFKYS
                     AVEGAAANALPDPVPEEIKQERLDRFMELAASISAEKLAAKVGRRLQVLVDHVDEDGA
                     VARSAADAPEIDGVVRIGRP"
     gene            1459..2163
                     /locus_tag="VD737_07260"
     CDS             1459..2163
                     /locus_tag="VD737_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HXW10395.1"
                     /translation="MHEVRRAEDRGHADHGWLESYHSFSFAGYHDPAHVQFGPLRVIN
                     EDRVVAGAGFGTHGHRDMEILSYVLEGELAHKDSTGTSSTIRPGDVQRMSAGTGVMHS
                     EFNASQDRPVHFLQIWILPDRLGIAPGYEERRFEAAEKRGRLRLIASPDREQGSVRIQ
                     QDARVYAGLLDGDEQARLDVAPGRRVYVHVARGQLAANGVALGAGDALKLSDTSQLEL
                     RGGRDAEVLVFDLPAE"
     gene            2277..2921
                     /locus_tag="VD737_07265"
     CDS             2277..2921
                     /locus_tag="VD737_07265"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXW10396.1"
                     /translation="MYTLYYSPGTASMAVHLALLEIGAPYELVEVEVPFDRQKDPSYL
                     RLNPRGQVPTLVVDGRPCFESSALLMLLAERHPEAKLAPQAGTPRRAEYYQWMAFLAT
                     ALGAPYRQWFYPGDLGAHDGHPLPDHVRDAVRHKIEGGWTIVDEHLAASGPYMLGAQF
                     SAVDLLALMYMRWSRNMPRPATTWPALRRYADSLRARPNWKKLCELEGLTEWAS"
     gene            complement(2964..3230)
                     /locus_tag="VD737_07270"
     CDS             complement(2964..3230)
                     /locus_tag="VD737_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXW10397.1"
                     /translation="MPTYDYHCPANGRVVEVSHKMAEKVSTWGELCRRVGLPLDGTSS
                     RARVEKLLGASGVVHATSLGSSRERPCDTGPCGGGGCGGGGCAF"
     gene            complement(3335..4654)
                     /locus_tag="VD737_07275"
     CDS             complement(3335..4654)
                     /locus_tag="VD737_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXW10398.1"
                     /translation="MNQTRSCHAALLGAVLSIAPLAGLAQATFHGNVARTGVFEGSGP
                     VSSPRTKWTFKGAGPIVTTPAVAGDAVYVASLSGHLYAIDRTTGAEKWNFKSRMAIAS
                     SPTVAGDTVYFVSSAGSLAAIDASNGQARWVYAVEHERRFEARGLHGYPPASQTIPDA
                     WDMWVSSPAVVDGRVYFGSGDGNVYAVEAKSGQLLWKFATGDVVHGSPAVANGVVYVG
                     SYDGKFYAIDAVTGQSRWTFQGGVDPAIHNQQGFQASAAVVDGVVYVGSRDAHVYALD
                     AATGRKKWDYPTSKSWVNATPAVRDGLVYAATSDSSRFFALDAKTGRLRFNFDAKAYV
                     FSSPALAGKLAYFGSHNGKLYAVDATTGAPAWEFQTEASRANADGLLLPDSRLNREAF
                     TPVFGDFQDMYVDFARFVSLGAFFGSPTVDRGVLYVGSTDGNLYALE"
     gene            complement(4781..5449)
                     /locus_tag="VD737_07280"
     CDS             complement(4781..5449)
                     /locus_tag="VD737_07280"
                     /inference="COORDINATES: protein motif:HMM:NF014007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HXW10399.1"
                     /translation="MLIEDGLLSDGIAVLASALGGVGLAYAIHGAPWKAWLASRERQW
                     VWFASMTVLLVVWSMKAGITPGLSVRFLLVSALTLMHGWHLAVVACALVLAVLTFVGG
                     AEWSSFGTNLLCSAIVPALFTAWLHEFVHDRLPHNYFVYFFVTGFFGAALAYNLAGLA
                     RVALIALSGTLETAHVGAEYFAILPLMSFGEAVANGMLLGMAVVYRSGWVMSFDDRLY
                     LGQR"
     gene            complement(5443..7962)
                     /locus_tag="VD737_07285"
     CDS             complement(5443..7962)
                     /locus_tag="VD737_07285"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HXW10400.1"
                     /translation="MKRASPSPMRGTRCSAIAVAWVGLTLALPAGTALAAGPLVSRVT
                     GEQEARAPTVEPVPAGEIPARADADERFAQDVIERAQRRNPTETLAPRLDTITAGIAD
                     LNDSLRREDLTRLPVVRLSSVENHWRFYDAELAKWRRELTRVMSPLADDAAALAERRA
                     IWDATFRSIGERGLPDALRARVGGMLTQITLAERALSGPLEAQIRLGQRANGVQSTID
                     DGRRHIEAAVSSYDRRLRSFDSPPLWRAWQETQLADEGPRGAGAGLRIERAFLAEWLA
                     ANPERLLAYQISVAALLPLLLWLAWRNRRVTTVDPALEGAARMLRRPLSAWLVLSFLG
                     LPFFFADAPLVLHQAALLLALLPVLRLLPPKVFEYLGPWPYIATALFVAYRLTFLLLG
                     QPLLYRLYLLALAVVTAGVLFWLLSNAVARDSGSTRLHGVVRTAGWLAFAALLASIAS
                     NVAGDVTLAEMLAGAVLDSAYIGLALFAGAHVLTSVLNMLMVRRSTSRFRVITQHTGP
                     LLAGLARLIGVAAFLIWLLATAYEFRVARPIFEGVRVVLTYPLEAGQISITLGSIALF
                     AFAVWLAFWVAKTVRVLLRDDVLPQMALPRGVGNSVSTLSYYALILLGLMVALAAAGF
                     EASQFALVFGALGVGIGFGLQNVVNNFVSGLILMFERPIQPGDVVEVSGTSGKVREIG
                     MRATTLTTFEGADVVVPNGTLLSEKLINWTLSDMNRRIDVEVGVAYGSDPRRVLALLA
                     EIASGTPGIVAEPTPAVVFKAFGPSSLDFGIRAWTHDFGDWVSIRTEMTARVYEALRR
                     EGIEIPFPQQDLNLRSVSPEAAARLGGLGGPGQPQPAPERC"
     gene            8107..8724
                     /locus_tag="VD737_07290"
     CDS             8107..8724
                     /locus_tag="VD737_07290"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HXW10401.1"
                     /translation="MHEMFSSLLEWYMNALDEGGYLLIALLMAMESSIIPLPSEVVIP
                     PAAYLAHAKGTFSLTGIVVAGTVGSWVGAAVMYWGSRWLGRPLLMRYGKYVAITPEKI
                     IKAEAWSSHYGMVGVFISRLLPVIRHLIGIPAGICRMHFGWYSLATVVGSAIWCTVLV
                     WLGVTAGQDEELMRGSLHRISLWGGGVLVFLGTLYYFFVHRHMRR"
     gene            8746..10206
                     /locus_tag="VD737_07295"
     CDS             8746..10206
                     /locus_tag="VD737_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXW10402.1"
                     /translation="MNESRERPTPGDVAAAAAPAWLPAQRRDPITGRVRVDYRAVAAL
                     ALPLMLNSSLQAVISLTDTWFVGHISTTAMAGMAAVYWVVLLFLMLVGGVGLAVQTFV
                     AQAQGSGRRARASHAAWIALWASALTVPLFAALAWSGAAIFGPFGLAPEVSAQALAFW
                     EPRMYGAPLGVALWAVAGFFNGISRPLIAVMTTGFVAIVNAVLNWLFVIELQWGIAGS
                     AWATNASMACGVVFGLCLFLGRDLRQRYRSHLTWRPDLRNLVRQYRLGLPMGAMYAAD
                     LFGMALFQLMQVRLSAVDGAATQIVMMLTSTVYMPGIGIALAGTTLVGQSIGAGDREW
                     ARRLGNTVIVLTTGFMGTIGVLLALAGPWLLPTFLGAGDPEAPEVVSLGVVLLWIAAG
                     YQIFDGLNLGSGFSLRGAGDVRLPAVLFFVLSWGVFVPLAHALSFAPGQGWIDLLPQF
                     GFGALGGWSALLVYVVLLGSALWLRWRSGKWRQARI"
BASE COUNT         1577 a         3525 c         3625 g         1553 t
ORIGIN      
        1 ggggcggccg atccgcacca cgccgtcgat ctcgggagcg tcggccgccg agcgcgcgac
       61 cgcaccgtct tcgtcgacgt ggtcgacgag cacctgcagt cggcgcccga ctttcgccgc
      121 gagcttctcc gcgctgatcg acgcggcgag ctccatgaac ctgtccagac gctcctgctt
      181 gatttcctcg ggcacgggat cgggcagcgc gttggccgcc gcgccttcga ccgccgaata
      241 cttgaaacag ccgacacgat cgaggcgcgc ctcgcgcagc cactccagca gcagctcgaa
      301 atcggcgtcg gtctcgccgg gaaacccgac gatgaacgtg ctgcgcagcg tcaggtcggg
      361 gcacacctcg cgccagcgcc ggatgcgctc gagcgtgttc tcggcatgcg cgggtcgctt
      421 catgagcttg agcacgcgcg ggctgccgtg ctggaacggg atgtccaggt atggcagcac
      481 cttgccttcc gccatcaacg ggatcacgtc gtcgacgtgc gggtacggat acacgtagtg
      541 cagccgcacc caggcgccga gcgtgccgag cgaccgcacg aggtccgcga accgcgcgcg
      601 gaccggcttg ccgccccaga accccgtcgc atacttgagg tcgacgccgt aggcgctcgt
      661 gtcctgcgag atcaccagca gttccttgac gccggccttc acgagacgct cggcctcgag
      721 catcacctcg ccgatcgggc gactcgccag gtcgccgcgc atcgacggga tgatgcagaa
      781 cgtgcagcgg tggttgcagc cctcggagat cttcaggtac gcgtagtggc gcggcgtgag
      841 ccggatgccc tgcggcggca cgaggtcgac gaacgggtcg tgcagcggcg gcagctgttc
      901 gtgcacggcc gcgacgactt cctcgtaggc gtgcgcgccg gtgaccttga gcacgctcgg
      961 gtggcggtcg gtgatcgtct cgggtcgtgc gccgaggcag cccgtcacga tgaccctgcc
     1021 gttgcgcgcc accgcttcgc cgatggcctc gagcgactca tgcacggcgt cgtcgatgaa
     1081 gccgcaggtg ttcacgacga cgaggttggc gtcctcgtac gagccgacga cgtcgtagcc
     1141 ttcggcctgc aggcgcgtga ggatgcgttc ggagtcgacc agcgccttgg ggcagccgag
     1201 cgagacgaag ccgatgcggg gcggggcgga ggacaaggaa gtgcctggcg gttagcggat
     1261 agcggttagt cggagcgcgc catgcgcgac ctagctctag ccctcgacgg gcaaggtcgg
     1321 ccgcaggccg gatgaggggg ccgccattct aacggttagg gtcgcttgat gccgccggtg
     1381 tgaagcgccg ggtcctcggc cgggtttaaa tcggccgcgg agaccccatc aggggacagt
     1441 cacacaggag atgcctccat gcacgaagtc cggcgcgccg aggaccgcgg tcatgccgat
     1501 cacggctggc tcgagtcgta ccactcgttc tcgttcgcgg ggtatcacga ccccgcgcac
     1561 gtgcagttcg ggccgctgcg ggtgatcaac gaggaccgcg tcgtggcggg cgcaggcttc
     1621 ggcacgcacg gccatcgcga catggagatc ctgagctacg tgctcgaagg cgagctggcc
     1681 cacaaggatt caacgggcac gagttcgacg attcgtcccg gggacgtgca gcgcatgagc
     1741 gccggcaccg gcgtcatgca cagcgagttc aacgcttctc aggaccggcc ggtccacttc
     1801 ctgcagatct ggatcctgcc ggaccggctc gggatcgccc cgggctacga ggagcggcgc
     1861 ttcgaggcgg cggagaagcg cggacgcctg cggctgatcg cgtcgcccga tcgcgagcag
     1921 ggctccgtca ggatccagca ggatgcgcgc gtctatgccg ggctgctcga cggcgacgaa
     1981 caggcccggc tcgacgtcgc gcccgggcga cgcgtctacg tgcacgtcgc gcgcggccag
     2041 ctggcggcca acggcgtcgc cctgggggcc ggcgacgcgc tcaagctcag cgacacgtcg
     2101 cagctcgagc tgcgcggcgg ccgcgacgcc gaggttctcg tcttcgacct cccggccgaa
     2161 taggggacag ccgaataggg gacactccga atttcccgtt aaatggggac actcctgatt
     2221 tcgcggggca tgctccgcga aaccggaccg tttccgcgtc cgcaggtcgc gccaccatgt
     2281 acacgctcta ctactcgccc ggcaccgcca gcatggccgt acacctcgcg ctgctcgaga
     2341 tcggcgcgcc gtacgagctc gtcgaggtcg aggtgccctt cgaccggcag aaggacccgt
     2401 cgtacctgcg actcaatccg cgcggccagg tgccgacgct cgtcgtggat ggacgcccgt
     2461 gcttcgaatc ctcggcgctg ctgatgctgc tcgccgaacg ccaccccgag gcgaagctgg
     2521 caccccaggc aggcacgccg cggcgcgccg agtactacca gtggatggcg tttctggcga
     2581 cggcgctcgg cgcgccttac cggcaatggt tctatcccgg cgacctcggc gcgcacgacg
     2641 gtcatccgct gccggaccac gtgcgcgacg cggtgcgtca caagatcgaa ggcggctgga
     2701 cgatcgtcga cgagcacctc gccgcgagcg gtccgtacat gctcggcgcg caattcagcg
     2761 cggtggacct gctcgcgctg atgtacatgc ggtggtcgcg caacatgccg cgcccggcca
     2821 cgacctggcc cgcgttgcgg cgttacgccg actccctgcg agcgcgtccg aactggaaga
     2881 agctctgcga gctcgagggg cttacggagt gggccagctg acggtccaga aggcgttcga
     2941 tctgccgcgc tgagcggcgg gcgctagaac gcgcagccgc ctccgccaca gcctccaccg
     3001 ccacaggggc cggtgtcgca cggtcgttcc cggctgctgc cgagtgacgt cgcgtgcacc
     3061 acgccgctcg caccgagcag cttttcgaca cgcgcgcggc tcgaggtgcc gtcgagcggc
     3121 agtccaacac gtcggcacag ctcgccccag gtcgaaactt tctcggccat cttgtggctg
     3181 acttcgacga cgcggccgtt ggccgggcag tggtaatcgt aggtcggcat ggagcgggtc
     3241 agctcgggct agggggccgc gacgattcta caacggccgt tcacggccgc cgaattcagg
     3301 agtgtcccca attaaccgga tttccggagt gtccctattc cagcgcgtag aggttgccgt
     3361 cggtgctgcc gacgtacagc acgccgcgat ccaccgtcgg cgagccgaag aacgccccga
     3421 gcgacacgaa gcgcgcaaag tcgacgtaca tgtcctggaa gtcgccaaag accggcgtga
     3481 acgcctccct gttgagccgg ctgtccggca gcagcagccc gtcggcgttg gcacgcgagg
     3541 cctcggtctg gaattcccag gcgggcgcgc ccgtcgtcgc gtcgacggcg tacagcttgc
     3601 cgttgtgact gccgaagtag gcgagcttgc cggcgagcgc gggcgacgag aatacgtacg
     3661 ccttcgcatc gaagttgaag cgcaggcggc cggtcttcgc gtcgagcgcg aagaaccgcg
     3721 agctgtccga cgtcgcggcg tagacgaggc cgtcgcgcac ggcaggcgtc gcattgaccc
     3781 acgacttgct ggtcgggtag tcccacttct tgcggccggt cgccgcatcg agcgcgtaga
     3841 cgtgcgcgtc gcgcgagccg acgtagacga cgccgtcgac gaccgcggcg gaggcctgga
     3901 atccctgctg attgtggatc gccggatcga cgccgccctg gaacgtccag cgcgactggc
     3961 cggtgaccgc gtcgatcgcg tagaacttgc cgtcgtagct gcccacgtag acgacgccgt
     4021 tggcgaccgc gggcgagccg tgcacgacgt cgccggtggc gaacttccac aacagctgtc
     4081 cactttttgc ctcgaccgcg tagacgttgc cgtcgccgct gccgaagtac acgcggccgt
     4141 cgaccaccgc cggcgacgac acccacatgt cccaggcgtc ggggatggtc tgcgacgccg
     4201 ggggatagcc gtgcaggccg cgggcttcga agcggcgctc gtgctcgacc gcatagaccc
     4261 agcgcgcctg gccgttcgac gcgtcgatcg cggcgagcga gcccgccgag gacacgaagt
     4321 agacggtgtc gccggcgacc gttggcgacg aggcgatcgc catccgcgac ttgaagttcc
     4381 atttctccgc gccggtcgtg cgatcgattg cgtacaggtg tccgctcagg ctcgcgacgt
     4441 acacggcgtc gcccgcgacg gcgggcgtgg tcacgatcgg tccggcaccc ttgaacgtcc
     4501 acttggtccg gggactcgag accgggccgg acccctcgaa cactcccgtg cgcgcgacgt
     4561 tgccgtggaa ggtcgcttgc gccaggcccg caagcggcgc gatcgacagg acggcgccga
     4621 gcagtgccgc gtgacagctc ctggtctggt tcatggcgag ggccccgtgc ggtcgaaagg
     4681 atcgcagcgg aaaccttacc gccaggctcg tgcgccggcg tggcgacggg gcaccgctcg
     4741 tcgcgacggt tgcccggctc ggcgcaacgg gccggcgtga ctagcgctga cccaggtaga
     4801 gccggtcgtc gaagctcatg acccagcccg agcggtacac gacggccatg ccgagcagca
     4861 tgccgttggc gacggcctcg ccgaagctca tcagcggcag gatcgcgaag tactcggcgc
     4921 ccacgtgcgc ggtctcgagc gtcccgctca gcgcgatcag tgcgacgcgg gccagtccgg
     4981 cgaggttgta ggcgagcgcc gcgccgaaga atcccgtcac gaagaagtag acgaagtagt
     5041 tgtgcggcag ccggtcgtgc acgaactcgt gcagccacgc ggtgaacagc gccggcacga
     5101 tcgccgagca gagcaggttg gtgccgaagc tggaccactc cgccccgccg acgaaggtga
     5161 gcaccgccag cacgagcgcg caggccacca cggcgaggtg ccagccgtgc atcagcgtga
     5221 gcgcggagac gagcaggaag cgaacggaaa ggcccggcgt gatgccggcc ttcatcgacc
     5281 agacgaccag cagcaccgtc atcgaggcga accagaccca ctgtcgctcg cggctcgcga
     5341 gccacgcctt ccagggcgct ccgtggatcg cgtaggcgag gccgaccccg ccgagcgccg
     5401 acgccaggac ggcgatgcca tcgctcagca ggccgtcttc gatcagcatc gttccggggc
     5461 gggttggggc tggccagggc cgccgaggcc gccgagccgt gcggccgcct ccggcgagac
     5521 gctgcgcagg ttgaggtcct gctgcgggaa cggaatctcg atgccttcgc gccgcagggc
     5581 ctcgtacacc cgggccgtca tctccgtacg gatcgagacc cagtcgccga aatcgtgggt
     5641 ccaggcccgg atgccgaaat cgagcgagct gggcccgaag gccttgaaga ccaccgccgg
     5701 cgtgggctcg gcgacgatgc cgggcgtacc ggacgcgatc tcggccagca acgcgagcac
     5761 gcgccgcgga tccgacccgt aggccacgcc cacctcgacg tcgatacgcc ggttcatgtc
     5821 gctcagtgtc cagttgatca gcttctcgga cagcagcgtc ccgttcggga ccacgacgtc
     5881 ggcgccctca aaggtcgtca gagtcgtcgc acgcatgccg atctcccgaa ccttgcccga
     5941 cgtgcccgag acctccacga cgtcgcccgg ctggatcggc cgctcgaaca tcaggatcaa
     6001 cccggacacg aagttgttga ccacgttctg caggccgaag ccgatgccga cgccgagtgc
     6061 gccgaagacc agcgcgaact ggctcgcctc gaagccggcc gccgcgagtg ccaccatgag
     6121 gcccagcagg atcagcgcgt agtagctcag cgtcgagacg ctgttgccga ccccgcgcgg
     6181 cagcgccatc tgcggcagca cgtcgtcgcg cagcaagacg cgcaccgtct tcgcgaccca
     6241 gaacgcgagc cagacggcga aggcgaagag cgcgatgctg ccgagcgtga tcgagatctg
     6301 cccggcctcg agcgggtagg tcaggaccac gcgcacgcct tcgaagatcg gccgtgccac
     6361 gcgaaactcg tacgccgtcg ccagcagcca gatcaggaac gccgcgacgc cgatcagccg
     6421 cgcgagcccg gcgagcagcg gtcccgtgtg ctgcgtgatc acgcgaaatc gcgaggtcga
     6481 gcgacgcacc atcagcatgt tgagcaccga cgtgagcacg tgggcgccgg cgaacagggc
     6541 caggccgatg tatgcgctgt cgagcacggc acccgcgagc atctcggcga gcgtgacgtc
     6601 gccggcgacg ttcgaggcga tgctcgcgag cagtgccgca aacgcgagcc agccggcggt
     6661 gcggaccacc ccgtgcagcc gcgtggatcc ggaatcgcgc gcgaccgcgt tggacaacag
     6721 ccagaagagc acgcccgcgg tcaccacggc cagcgcaagc aggtacagcc ggtacagcag
     6781 gggctgtccg agcaacagga acgtgagccg gtacgccacg aacaacgcgg tcgcgatgta
     6841 cggccacggc ccgaggtact cgaacacctt cggcggaagc agccgcagca ccggcagcag
     6901 cgccagcagc agtgccgcct ggtgcagtac gagcggtgca tccgcgaaga agaacggcag
     6961 ccccaggaac gacagcacga gccacgccga cagcggccgg cgcaacatgc gggcagctcc
     7021 ctccagcgcg ggatcgacgg tcgtcacccg gcggttgcgc cacgcgagcc agagcagcag
     7081 cggcaacagc gccgcgacgc tgatctggta cgcgagcagc cgctccgggt tcgcggcgag
     7141 ccattcggcc aggaaggccc gctcgatgcg cagccctgcc cccgcacccc gcggcccctc
     7201 gtcggcgagc tgcgtctcct gccaggccct ccagaggggt ggcgagtcga aggagcgcag
     7261 gcggcggtcg tacgaactga ccgccgcctc gatgtgccga cgcccgtcgt cgatcgtcga
     7321 ttgcacgccg ttcgcgcgct ggccgagccg gatctgcgcc tcgagcggcc cggagagcgc
     7381 ccgctccgcg agcgtgatct gcgtcagcat gccgccgacc ctggcgcgca gggcgtccgg
     7441 caggccgcgc tcgccgatcg accggaaggt ggcgtcccat atcgcgcggc gctcggcgag
     7501 ggccgctgcg tcgtcggcca ggggcgacat cacgcgcgtc agttcgcgtc gccacttggc
     7561 gagttccgcg tcgtagaagc gccagtggtt ttcgacgctg ctgaggcgta cgaccggcag
     7621 ccgggtcaga tcttcgcggc gcagcgagtc gttgagatcg gcaatccccg ccgtgatcgt
     7681 gtcgaggcgc ggcgccagcg tctcggtcgg attgcgccgt tgcgcgcgct cgatgacgtc
     7741 ctgggcgaag cgctcgtccg cgtcggcccg cgccgggatc tcgccggccg gcaccggctc
     7801 gaccgtgggc gccctggcct cctgctcgcc cgtgacgcgg ctgacgaggg gtccggccgc
     7861 cagggccgtc cccgccggca gcgccagcgt gaggccgacc cacgcgacgg cgatggcgga
     7921 gcaacgggtg ccgcgcattg gcgacgggga cgcacgcttc atttcggtat ccgtttggcc
     7981 gcagcggtgc aattgtcgca cggccgcgac ggcgggcccg ccggcacggg gctctggcag
     8041 aatgcgcgac tcgttgcgcg acgccccgtc gatgcgcgac gcccccgttc cgagcgagtc
     8101 cgcgccatgc acgagatgtt ctcttccctg ctcgagtggt acatgaacgc gctcgatgag
     8161 ggcggatacc tgctgatcgc gctgctgatg gccatggaga gttcgatcat cccgctgccg
     8221 agcgaggtcg tgatcccgcc ggccgcgtac ctcgcgcacg ccaagggcac gttttcgctg
     8281 acgggcatcg tcgtcgcggg gacggtgggt tcgtgggtcg gcgcggccgt gatgtactgg
     8341 ggatcgcgct ggctcgggcg cccgttgctg atgcgttatg gcaaatacgt cgccatcacg
     8401 ccggagaaga tcatcaaggc cgaggcctgg tcgtcgcact acggcatggt cggggtcttc
     8461 atttcacgac tcctgcccgt gatccgccac ctgatcggca tcccggcggg catctgccgc
     8521 atgcacttcg gctggtattc gctggcgacg gtggtcggct cggccatctg gtgcacggtg
     8581 ctggtgtggc tgggcgtgac cgcgggccag gacgaagagt tgatgcgcgg cagcctgcac
     8641 cgcatctcgc tgtggggtgg tggcgtgctg gtcttcctgg gcacgctgta ctacttcttc
     8701 gtgcaccggc acatgcgccg ctgagggcgc gccgccgggg aaaccatgaa cgagtcacgc
     8761 gaacgcccga cgcccggcga cgtggccgcg gcggcggcgc ccgcgtggct gccggcgcag
     8821 cggcgcgatc cgatcacggg tcgcgtacga gtcgactacc gggccgtcgc ggcgctggcg
     8881 ctgccgctga tgctcaacag ctcgctgcag gccgtcatca gcctgacgga cacctggttc
     8941 gtcggtcaca tctcgaccac cgccatggct ggcatggccg ccgtgtactg ggtggtgctg
     9001 ctgttcctga tgctggtcgg tggcgtcggc ctcgcggtgc agaccttcgt cgcccaggcg
     9061 cagggcagcg gtcgacgcgc acgtgcgagc cacgcggcct ggatcgcgct ctgggcgagc
     9121 gcgctcaccg tcccgctgtt tgcggcgctc gcgtggtccg gtgcggcgat cttcgggccg
     9181 ttcgggctcg cgcccgaggt ctcggcacag gcgctcgcgt tctgggagcc acgcatgtac
     9241 ggcgcgccgc tcggcgtcgc gctgtgggcc gtcgcggggt tcttcaacgg catctcccgg
     9301 ccgctgatcg ccgtcatgac gaccggcttc gtggcgatcg tcaacgccgt gctcaactgg
     9361 ctgttcgtga tcgagctgca gtggggcatc gccggctcgg cctgggcgac gaacgcgagc
     9421 atggcgtgcg gcgtggtgtt cggactctgc ctgttcctgg gccgcgacct gcgccagcgt
     9481 tatcggagcc acctcacgtg gcgacccgac cttcggaacc tggtgcggca gtaccggctc
     9541 ggcctgccga tgggagcgat gtacgcggcc gacctgttcg gcatggcgct gttccagctg
     9601 atgcaggtgc ggctgagcgc cgtggacggg gcggcgacgc agatcgtcat gatgctgacg
     9661 tcgaccgtgt acatgcccgg catcggcatc gcgctcgcag gcacgacgct ggtcggccag
     9721 tcgatcggcg cgggcgaccg cgagtgggcg cgccgtctcg gcaacacggt gatcgtgctg
     9781 acgacgggtt tcatgggcac gatcggcgtg ctgctcgcgc tcgccggccc gtggctgctg
     9841 ccgacgttcc tgggcgcggg cgaccccgaa gcgcccgagg tcgtctcgct cggcgtcgta
     9901 ctgctctgga tcgcggccgg ctaccagatc ttcgacgggc tcaacctcgg cagcggcttc
     9961 tcgctgcgcg gggcggggga cgtacgcctg cccgcagtac tgttcttcgt gctgtcgtgg
    10021 ggcgtgttcg tgccgctcgc gcatgcgctg tcgttcgcgc cgggccaggg ctggatcgac
    10081 ctgctgccgc agttcgggtt cggcgcgctc ggcggctggt cggcactgct cgtgtacgtc
    10141 gtgctgctcg gcagcgcgct gtggttgcgc tggcggtctg ggaagtggcg gcaggccagg
    10201 atctagtgac cagtgttcgg cgcagaaaag cccgacctct cgggtcttac gagccactaa
    10261 ccactagcca ctagccacta
//
LOCUS       DASPFR010000273         2269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1053224, whole genome shotgun sequence.
ACCESSION   DASPFR010000273 DASPFR010000000
VERSION     DASPFR010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053224"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VD737_07300"
     CDS             complement(<1..574)
                     /locus_tag="VD737_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HXW10403.1"
                     /translation="MLASFACALALAGCGGGGDGGGGSAPPDGGGNGGGNPPPSATID
                     FRDEVVYQLLTDRFHNGDAGNDGGDLSRRGDAADPTNPVGWHGGDFAGIRQKIQDGYF
                     QQLGVTALWISPVVRQVAAAGNGGGVNSSRPFAGFHGYWADRFDAIEPHFGNLAELAA
                     LGDAAEAAGLALVIDVVVNHTGPNSSVLASN"
     gene            complement(607..1905)
                     /locus_tag="VD737_07305"
     CDS             complement(607..1905)
                     /locus_tag="VD737_07305"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXW10404.1"
                     /translation="MRTTATFVACTIVLAAGTAVAAEPLALQCGDTFDSRTAKLAGAQ
                     TIVTGTDGRIAQVLPGKAAPAGAGVVDLSTSTCLPGLIDLHVHLDTQSNPKSYEEGFR
                     LDDIDFGYRALAYADRTLRAGFTTVRDLGGEVTLHLRDAINQGYVQGPRIYAAGKSIA
                     TTGGHADPRNGLNSELAHVLGPPGPTEGVVNGPDDARQAVRQRYKDGSDLIKITATGG
                     VLSYARSADAPQFTIEEIRTIVETAKDYGYRVAAHAHGKEGMRRAIEGGVTSIEHGTY
                     MDEEIFALMKKHGTWYVPTISAGRFVAEMAKQDGYYPAIVRPKAEQVGAQIQKTFGKA
                     YRAGVRIGFGTDQGVAPHGDNAREFAYMVEAGMPANVALQSATFNAAEVLGAPELGQL
                     APGFHADVVAVPGNPVEDVALMTRVGFVMKDGVIVVGPTP"
BASE COUNT          326 a          783 c          786 g          374 t
ORIGIN      
        1 gattggacgc gagcacggac gagttcgggc cggtgtggtt cacgacgacg tcgatcacca
       61 gcgcgagccc tgccgcctcg gccgcgtcgc cgagcgcggc gagttcggcg aggttgccga
      121 agtgcggctc gatcgcgtcg aaccggtcgg cccagtagcc gtggaagccg gcgaagggac
      181 ggctcgagtt gacgccgcca ccgttgccgg cggcagccac ctgtcgcacg accggcgaga
      241 tccagagcgc ggtgacgccg agctgctgga agtagccgtc ttggatcttc tggcggatgc
      301 cggcgaagtc gccgccgtgc cagccgaccg gattggtcgg gtcggccgcg tcgccgcgtc
      361 gcgagagatc gccaccgtcg ttgcccgcgt cgccgttgtg aaagcggtcg gtcaacagct
      421 ggtagacgac ctcgtcgcgg aaatcgatcg tggccgacgg tggcggattg ccgccgccgt
      481 taccgccgcc gtccggcgga gcactgccgc ccccaccgtc cccaccaccg ccgcagccgg
      541 cgagcgccag cgcacaggcg aacgacgcca gcacgtgtcg accaggctca cgcctgtcac
      601 gcggtctcac ggcgtgggcc cgacgacgat cacgccatcc ttcatcacga agccaaccct
      661 ggtcatcagc gcgacgtcct ccacgggatt gccgggcacc gccaccacgt cggcgtggaa
      721 gcccggtgcg agctgcccga gctccggtgc gccgagcacc tcggcggcgt tgaaggtcgc
      781 gctctgcagc gcgacgttcg ccggcatgcc cgcctcgacc atgtacgcga attcacgcgc
      841 attgtcgccg tgcggcgcga cgccctggtc cgtgccgaag ccgatcctga cgcccgcgcg
      901 gtaggccttg ccgaacgtct tctggatctg cgcgccgacc tgctccgcct tcggccgcac
      961 gatggccggg tagtagccgt cctgcttcgc catctcggcg acgaacctgc cggccgaaat
     1021 cgtcggcacg taccacgtgc cgtgtttttt catcagcgcg aagatttcct cgtccatgta
     1081 cgtgccgtgc tcgatcgagg tgaccccgcc ctcgatcgcg cgccgcatgc cttccttgcc
     1141 gtgggcgtgc gcggcgacgc gataaccgta gtccttcgcc gtctcgacga tcgtgcggat
     1201 ctcttcgatc gtgaactgcg gcgcgtcggc gctccgggcg tagctcagca cgccgccggt
     1261 ggcggtgatc ttgatcaggt cggagccgtc cttgtagcgc tgccgcaccg cctggcgcgc
     1321 gtcgtcgggg ccgttgacga cgccctcggt cggcccgggc gggccgagca cgtgcgcgag
     1381 ttctgagttc aggccgttgc gcggatcggc atgaccgccg gtcgtggcga tcgacttgcc
     1441 cgccgcgtag atccgcggtc cctgcacgta gccctggttg attgcgtcgc gcaggtgcag
     1501 cgtcacctcg ccaccgaggt cgcgcacggt cgtgaaaccg gcccgcagcg tgcggtccgc
     1561 gtatgcgagc gcgcggtagc caaagtcgat gtcgtcgaga cggaagccct cctcgtacga
     1621 cttcgggttc gactgggtat cgaggtgcac gtgcaggtcg atcaggccgg gcaggcaggt
     1681 cgaggtcgac aggtccacca cgcctgctcc cgccggcgct gccttgccgg gcagcacctg
     1741 cgcgatgcga ccgtcggtcc cggtcacgat cgtctgcgcg ccggcgagct tcgcggtgcg
     1801 cgagtcgaac gtgtcgccgc actggagcgc gaggggttcc gcggcgacgg cggtgcccgc
     1861 ggcgaggacg atcgtgcagg cgacgaacgt cgcggtcgtg cgcatcgatg gaggctccct
     1921 tggcaaagat cggcgattct acgtcgtccc tctcgccatg acgggtgccg gcgcgcgatg
     1981 acggtccgct gcgacttcaa cgaccagtcg caggaaaaca acgcaccatc gcctgtattg
     2041 agtctcgatt tgcgtttcgc caacagtagc ggacaatgcg ggcgtcgtct cacacaaggg
     2101 gaagtgggtc catggaatgc acgaatcgac tgcaacgggc catccgtctg gccttgggcg
     2161 gtgccgtagg gctgggctgc ggactggcgg gcctgcccgc gctgggccag ggaaccgggg
     2221 acggtctcga aaccgtctac gtgaccggtt cccgcatcgc gaggacttc
//
LOCUS       DASPFR010000274         3003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_199170, whole genome shotgun sequence.
ACCESSION   DASPFR010000274 DASPFR010000000
VERSION     DASPFR010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199170"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..>3003
                     /locus_tag="VD737_07310"
     CDS             15..>3003
                     /locus_tag="VD737_07310"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HXW10405.1"
                     /translation="MAPDAPVGVVSAELHASGTDRTAAVQGELGVDGETVHVVDGRLV
                     LRPEPRTLHLERLVLRLNEEPGQFEVAGTVALDADPVTADLKTHWRELTIPEAWAGQQ
                     LHTSGQLAVRGSAERYALSGEIEVGPPGRLADVSVVAQGTPRRLALEKFVVEQEAGRL
                     EAEGALDLQPQRGWSLTARARDFNPGAIFADWPGRLHVDLRTEGVMEADGPRGTVQLD
                     ELRGSLRGRRLEGGGKLEFALPSSLAGTLRLRSGDSTVRVDAEPGDALDVRARVRVAD
                     VGDFLPESRGRVDADVTVRGRWPDVRIAGVVDGDALGLASTSARHVRLEVDVRNPIDP
                     RGKAELVAREITAGELRFAEVRAEASGTRAEHRFTLDASGETLDVATRVSGSLAADDS
                     WSGRVASLELGVLDDVRLDLQRPVAISYSADGLQVDEACLRTRREGAQLCAALSSRAD
                     GTLRADYRLRDVPLSLASLAAPDALPGELDGTIAGQGQVRRGSDALWYGELALESDRA
                     RIVWSDEGPAAVAGQPLLIYEGLRAEARLDGSRASARMTAAIGGEGSVTANVNLTALD
                     TAAPRLSGRVQADLPTLDPIAPFVPKVAGLQGRVEVDARLAGTTAEPLFSGYVRAAQL
                     AGDVPMLGLQLRDGSLTAESRTDGRITLEGTVRSGRGRLTLDGTATRSGELDATINGR
                     RFVAADMEGARVVAAPDLRFTRNADGMELTGEVRIEEAKINLQELPRSKPGARSVSPD
                     VVIVNEPPPTAAEEAASSLPLTATVTVVLEEEAVELAGYGLDATVDGQLTVRDRPGRV
                     TTASGELLIDGTYKAYGQDLTIREGEVRYAGTPVDNPRLRIEAVREVDDVIAGLRITG
                     SAQDPQIDVFSDPSLGETDALAYLVTGRPMSAIGTAGEGDSDLMQEASQSLGTAAGGL
                     LAKRLGQRLGIDEFGIAKSEEIGGAALTIGQYLSPRLYVGFGVGLFDPGQLVTLRYDV
                     SESVSVEAVTSDETTRAGIDYRIE"
BASE COUNT          427 a         1051 c         1117 g          408 t
ORIGIN      
        1 accttcgcgc agagatggcc ccggacgcgc cggtgggcgt ggtgtcggcc gagctgcacg
       61 ccagcggcac cgatcgtacc gctgccgtgc agggcgaact gggcgtggac ggcgagaccg
      121 tgcacgtcgt cgacggtcga ctcgtgctgc ggccggagcc tcgaacgctg cacctcgagc
      181 gcctcgtgct gcggctcaac gaggagcccg gccagttcga ggtggccggc acggtcgctc
      241 tcgatgccga ccccgtcacg gcggacctga agacgcactg gcgcgagctc acgattccgg
      301 aggcctgggc gggacagcaa cttcacacca gcggccaact ggccgtgcgt ggcagcgccg
      361 agcgctatgc gttgagcggt gaaatcgagg tcggtccgcc cggccgattg gccgacgtca
      421 gcgtcgtcgc ccagggcact ccgcggcggc tcgcgctcga gaagttcgtc gtggagcagg
      481 aggccggccg gctcgaggcc gagggcgcgc tggacttgca gccgcaacgc ggctggtcac
      541 tgacggcgcg cgcacgcgac ttcaatccgg gcgcgatctt cgccgactgg cccggtcggc
      601 tgcacgtcga cctgcgcacc gaaggcgtaa tggaggccga cggcccgcgc ggcaccgtgc
      661 aactcgacga gctgcggggg agcttgcgcg gtcgtcggct ggagggcggc ggaaaactgg
      721 aattcgcact gccgtcctcg ctcgccggca cgctccggtt gcgctccggc gacagcaccg
      781 tgcgggtcga cgccgagccc ggcgacgcgc tcgacgtacg cgcccgggtt cgcgtcgccg
      841 acgtgggcga tttcctgccc gaatcgcgcg gtcgcgtgga cgccgacgtg accgtgcgcg
      901 gccgttggcc ggacgtgcgc atcgcaggcg tggtcgacgg cgatgccctc ggcctcgcat
      961 ccacgagcgc tcgtcacgtg cgcctcgagg tcgacgtgcg caacccgatc gatccacgcg
     1021 gcaaggcgga gctggttgcg cgggagatca ccgccggcga actgcgcttc gcagaagtgc
     1081 gcgccgaggc cagcggcact cgtgccgagc accgcttcac gctggatgcg tcgggcgaaa
     1141 cgctggacgt ggcgacgcgt gtgagcggct cgctggccgc ggacgactcc tggtcgggtc
     1201 gtgtcgcgag tcttgaactc ggcgtccttg acgacgtgcg cctcgacctg cagcggcccg
     1261 tggcgatcag ctactccgcc gacgggctcc aggtcgacga ggcgtgcctg cgcacgcgac
     1321 gcgagggagc gcagctctgc gcggccctga gcagccgggc cgacggcacc ctgcgggccg
     1381 actatcgcct gcgcgacgtg ccgctgtcgc tggcgagcct cgcggcgccg gacgcgttac
     1441 ccggcgaact cgacggcacg atcgcgggcc agggccaggt gcgtcggggg agcgacgcac
     1501 tctggtacgg tgaactcgcg ctcgagtccg atcgcgcccg gatcgtgtgg tccgacgagg
     1561 gcccggcggc cgtcgcgggc cagccgctgc tgatctacga gggcctgcgc gccgaggccc
     1621 ggctcgacgg ctcacgcgca agcgcgcgca tgacggcagc gatcggcggt gaaggttcgg
     1681 tcaccgccaa cgtgaacctg accgcgctcg acacggccgc gccacgcttg tcggggcgtg
     1741 tgcaggccga cctgccgacg ctcgatccga tcgcgccgtt cgttccgaag gtggcggggc
     1801 tgcagggccg cgtcgaggtc gacgcgcgtc tcgctggcac tacggccgag ccgctgttca
     1861 gcggctacgt gcgcgccgcg cagctcgccg gcgacgtgcc catgctcggc cttcagctgc
     1921 gcgacggctc gctgacggct gagtcgcgca ccgacgggcg cattacgctc gagggcacgg
     1981 tccgttcggg gcgcgggcgg ctcacgctcg acggcacggc aacccgctcc ggggagctcg
     2041 atgcaacgat caacgggcga cgcttcgtcg cggcggacat ggagggtgcg cgcgtggtcg
     2101 cggcgcccga cctgcgcttc acgcgcaacg ccgacggcat ggaactgacc ggcgaggtcc
     2161 ggatcgagga agcgaagatc aacctgcagg agctgccgcg gagcaagccc ggcgcgcgct
     2221 cggtgtcccc ggacgtcgtg atcgtgaacg agccgccgcc gaccgccgcg gaggaagccg
     2281 cttcgtccct gccgttgacg gcgaccgtga ccgtggtcct ggaggaggag gcggtcgagc
     2341 tggccggata cggactcgac gccaccgtcg acggccagct caccgtccgc gaccgccccg
     2401 gccgcgtgac gacggcctcg ggcgagctct tgatcgacgg gacgtacaag gcctacgggc
     2461 aggacctgac gatccgcgaa ggcgaggtgc gctatgccgg cacgcccgtc gacaacccgc
     2521 gcctgcgcat cgaggccgtg cgggaggtcg acgacgtgat cgcgggcctg cggatcacgg
     2581 gctccgcgca ggacccccag atcgacgtgt tctcggatcc ttcgctcggc gagaccgacg
     2641 cgctcgccta cctcgtgacg ggccgcccaa tgagcgcgat cggcacggcc ggcgaggggg
     2701 acagcgacct gatgcaggag gcttcgcaat cgctcggcac ggcggccggt ggactgctgg
     2761 ccaagcgcct cggccagcga ctcggcatcg acgagttcgg catcgcgaag agcgaggaaa
     2821 tcggcggcgc cgcgctcacg atcggccagt acctgtcgcc gcggctctac gtcggcttcg
     2881 gcgtggggct gttcgacccc ggccagctcg tgacgctgcg ttacgacgtc tccgagtccg
     2941 tctctgtcga ggccgtcacc agcgacgaga cgacgcgcgc cgggatcgac tatcgaatcg
     3001 agc
//
LOCUS       DASPFR010000275         6130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_199318, whole genome shotgun sequence.
ACCESSION   DASPFR010000275 DASPFR010000000
VERSION     DASPFR010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199318"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /gene="modA"
                     /locus_tag="VD737_07315"
     CDS             <1..799
                     /gene="modA"
                     /locus_tag="VD737_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HXW10406.1"
                     /translation="APGGDVMRGNAWRISWLACLIAVFPSIAAPATPARVYAAASLTQ
                     ALTEIATLWQRAGHPAPVLVFAGSATLARQLEAGAAADVFISADSAWLDALAKSGRVD
                     AASRTDLLGNALVLIAPQTAPFRIDLRPGAPLGQAFEGRLCTGEPGVVPVGTYARAAL
                     QWLGAWDSLTTRLVGTEDVRAALALVERGHCGAGIVYATDARGSTRVVAVGTFPATSH
                     RPIVYPAALLRGATPQGRAFFEFVRDSQAAAVVFERVGFTRLGKAPR"
     gene            804..1520
                     /gene="modB"
                     /locus_tag="VD737_07320"
     CDS             804..1520
                     /gene="modB"
                     /locus_tag="VD737_07320"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HXW10407.1"
                     /translation="MLSAAEWDALGLSARVAAWATLASLPFGVWAGWLLARREFRGKF
                     VLEALVQLPMVVPPVVPGYLLLWLLGPGAPLGGWLESRFGLSVAFSWKGAVIASAVMA
                     FPLLVQPVRVAFRLIDVRLEHAASTLGASPWNAFRTVALPLAMPGVLAGAVLCFSRSL
                     GEFGATMAFVGNIPGETRTLPLAIYSFMHVPGGEAAAARLVALSLLLAFAALAGSHWL
                     TRRAERRLGYLDQDVRGPGA"
     gene            1513..2157
                     /locus_tag="VD737_07325"
     CDS             1513..2157
                     /locus_tag="VD737_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXW10408.1"
                     /translation="MLSFDLEFARAGFALQAQAEFGAGATGIFGPSGCGKSTLLSLVA
                     GLLRPQRGVMALDRTTLVDVERGVFVPAWRRHVALVFQDGQLFPHMTVRANLLYGHDR
                     RAPSDRRLQLPQVVDLLELGPLLDRRPAQLSGGERQRVALGRALLYSPRLMLLDEPLA
                     SLDDRLKQQILPFLRRVKDESGIPMLYVSHVAAEVEFIADRVLRMERGRIVGIT"
     gene            2287..3381
                     /locus_tag="VD737_07330"
     CDS             2287..3381
                     /locus_tag="VD737_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXW10409.1"
                     /translation="MATILVVDDRREHRKATRQVLERAGHYVIETHDGHDALERARFE
                     KPDLVLSDVLMPGMDGFTLCRRAQEDEALRHVPFVFMTGTFADPRYAQFAAELGAVDV
                     LPKPLDARSLRAAVDRALLRGAAPDATERLRTLDDGTFHARHIGVVTTKLEEKIAELE
                     SANAELRRGELRLRDMLGAVVSTISKMVELRDPYTTGHERRVGDLAIAIGTVLGFDAD
                     RLDGLRIGGYLHDVGKIAMPSSILSKPGRLSDVELALIRAHAQIGHEVLSGIDFPWPI
                     AEMTWQHHERLDGSGYPRGLRGDEILLEARILAVADTVEAMTSHRPYRPAVGLALALE
                     EIERGRGVLYDPEVVDACLQLFREGRFAFD"
     gene            3513..3842
                     /locus_tag="VD737_07335"
     CDS             3513..3842
                     /locus_tag="VD737_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10410.1"
                     /translation="MSVKSMAAIVAAVAMTVVAARDLRPAELARSPWLRNAVPVHEAL
                     PPGHPPIPGQRLPQGHPPVERAYPDLPPGHPPIPWAQPGCPGQFGFPDEGIGVGFGRD
                     ERAVIST"
     gene            3972..4703
                     /locus_tag="VD737_07340"
     CDS             3972..4703
                     /locus_tag="VD737_07340"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HXW10411.1"
                     /translation="MLSPRIARLGHRWLALLVGWQLAIWFLSGLYMVVVDLDFIHGDS
                     LVRNVAPPVRLDEAIAPLAAIRAGRDDVQAIRMRVLPDRDQPVYEVVRTSGTELFDAG
                     TGRPMPVLDEARIRALAQAYYAGRGSIAGAQLLTNAADLPGEIRGRRPPVWQIDFDDW
                     TATSLYVDPVTGRLVTRRHRFWRWFDFLWSLHIMDYVEREDVNNPLLRIATPLAFVTV
                     AFGGWLAFHSFGFLQRRRMRRRGAA"
     gene            4700..5416
                     /locus_tag="VD737_07345"
     CDS             4700..5416
                     /locus_tag="VD737_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10412.1"
                     /translation="MKLLRLRKLHRWVALVVGLQVVVWTASGFAFAWLDHHDVTGEAL
                     AAAPAPRSLPADVTVLDPARLPIAQATTTVASLELRDLDGTWVYRIETVDGRVSLLRA
                     SDGEPFAIDAATTRRLAGRHYRGTGRLQEVRAQPAGTGETRGLGATWQATYDDALGTS
                     LHFSAADGALVAVRTDAWRLKDFFWMLHTMDYRGRDDFNNPLVVTAGAAAAWVGLTGI
                     WLLLRVFRRPAEADGDRLRT"
     gene            complement(5406..>6130)
                     /locus_tag="VD737_07350"
     CDS             complement(5406..>6130)
                     /locus_tag="VD737_07350"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HXW10413.1"
                     /translation="ELEPLQARAFGGSLVTHLRYDYAAPEPTLSVRQELAGVDVAALL
                     GQLFDVRQLEGRGSGRFALTSRGADGASLFANLRGPFDVEVTDGAVLGVDLWHELERA
                     VAAAQLEAAAATGRGSGRTPFERLSARGTLIERSLRNDRVEFVADFARVRGRGEVDYG
                     RNALDLDLTARLLKAPKGRLLGIKVSRVKDADIPLDVTGTLAEPKVRPDVSKLLEAAA
                     KDAIKQPLEGKIRDELEKIFKF"
BASE COUNT          787 a         2180 c         2180 g          983 t
ORIGIN      
        1 cgccccggga ggagacgtga tgcgcggcaa cgcgtggcga atctcgtggc tcgcgtgcct
       61 gatcgcggtc tttccgtcga tcgccgcccc ggcgaccccg gcccgcgtct acgcggccgc
      121 gagcctcacg caggctctca ccgagatcgc gacgctctgg cagcgcgcag gacacccggc
      181 gcccgtgctc gtcttcgcgg gttccgcgac gctcgcgcgg caactggaag ccggcgccgc
      241 cgcggacgtg ttcatctcgg cggactcggc ctggctggac gcgctcgcga agagcggtcg
      301 agtcgacgcc gcgagtcgaa ccgacctgct cggcaacgca ctggtgctga tcgctccgca
      361 aacggcgccc ttccggatcg acctgcgccc cggcgcgccg ctcgggcagg cgttcgaggg
      421 gcggctctgc accggcgaac ccggcgtcgt gccggtgggc acatatgcgc gagcagccct
      481 gcagtggctc ggcgcgtggg attcgctcac gacgcggctc gtcggcaccg aggacgtgcg
      541 cgccgcactc gcgctcgtgg agcgcggcca ctgcggcgca ggcatcgtct atgcgacgga
      601 tgccaggggc agcacgcgcg tggtcgcggt gggcacgttc ccggcgacgt cgcatcgccc
      661 gatcgtctat ccggccgcgc tgctgcgtgg ggcgacgccg caaggccggg ctttcttcga
      721 gttcgtgcgc gactcccagg ctgccgcggt cgtgttcgaa cgggtcggct tcacgaggct
      781 ggggaaagcg ccacgatgaa cgcatgctga gcgcggccga gtgggatgcg ctcgggctct
      841 cggcccgggt cgccgcctgg gcgacgttgg cgtcgctgcc gttcggcgtc tgggcgggat
      901 ggctgctcgc gcggcgcgag ttccgcggca agttcgtgct cgaggcgctc gtgcagctgc
      961 cgatggtggt gccacccgtc gtcccgggct acctgctcct ctggctgctc gggccgggcg
     1021 ctccgttggg cggctggctc gagtcgcggt tcgggctttc ggtcgcgttc tcctggaagg
     1081 gcgccgtgat cgcctcggcg gtcatggcat tcccgctgct ggtgcagccg gtgcgcgtcg
     1141 cgttccgcct gatcgacgtg cggctcgagc acgctgcctc gacgctcggc gcctcgccct
     1201 ggaacgcgtt tcgcacggtc gccctgccgc tcgcgatgcc gggcgtgctg gcaggcgcgg
     1261 tgctgtgctt ttcgcgcagc ctcggcgagt tcggcgccac catggcgttc gtcggcaaca
     1321 tccccggcga gacacggacg ctgccgctcg ccatctacag cttcatgcac gtccccggcg
     1381 gcgaggccgc cgccgcgcga ctggtggcgc tgtcgctcct gctggccttc gcggcgctcg
     1441 cgggcagcca ctggctcacc cggcgcgccg agcgacggct cggttacctc gaccaggacg
     1501 tgcgggggcc cggtgcttag cttcgacctg gaattcgccc gcgcgggctt cgcgctgcag
     1561 gcgcaggccg agttcggtgc gggcgcgacc gggatcttcg gcccctccgg ctgcggcaag
     1621 agcacgctgc tgtcgctggt cgcgggactg ctgcgtccgc agcgcggcgt catggcgctc
     1681 gaccggacga cgctggtcga cgtcgagcgt ggcgtcttcg tgccggcgtg gcgtcgccac
     1741 gtcgcgctgg tgttccagga cgggcagctc tttccgcaca tgaccgtccg cgccaacctg
     1801 ctctacggcc acgaccgccg cgcgccgtcg gaccgccggc tgcaactgcc gcaggtcgtc
     1861 gacctgctgg agctcgggcc gctgctcgac cggcggccgg cgcagctgtc gggcggcgag
     1921 cgtcagcgcg tcgcgctcgg gcgggcgctg ctgtattcgc cacggctcat gctcctcgac
     1981 gagccgctgg cgtcgctgga cgaccgcctg aagcagcaga ttcttccttt tctgcgccgc
     2041 gtcaaggacg agagcggcat cccgatgctc tacgtctcgc acgtcgcagc ggaggtcgag
     2101 ttcatcgccg atcgcgtcct gcgcatggag cgcggccgta tcgtgggaat cacttaggtt
     2161 tcgcgggcga tcctccaggc gccccgcggc tgaccacggc ctgccgcgtc gatcctccca
     2221 aaacggttcc tgcctgccga ggcccggtgc gccgtcagcg tagattggcc tgccctagga
     2281 ggttccatgg ctacgatcct ggtcgtcgac gatcgccgcg agcaccgcaa ggccacgcgc
     2341 caggtgctcg aacgcgccgg ccactacgtc atcgagacgc acgacggtca cgacgcactc
     2401 gagcgcgcgc gtttcgagaa gccggacctc gtgttgtccg acgtgctgat gccaggcatg
     2461 gacggcttca cgctgtgccg tcgtgcgcag gaggacgagg cgctgcgcca cgtgccgttc
     2521 gtgttcatga cgggtacgtt cgccgacccg cgctacgcgc aattcgccgc ggaactcggg
     2581 gccgtggacg tgctgccaaa gccgctcgac gcgcgctcgc tgcgcgccgc cgtcgaccgc
     2641 gcgctgctgc gcggcgccgc gcccgatgcc acggagcggc tcaggacgct ggacgacggg
     2701 accttccacg cgcgacacat cggcgtcgtc accaccaagc tcgaggagaa gatcgccgag
     2761 ctggagtccg ccaacgccga gctgcgccgc ggcgagctgc gcctgcgcga catgctcggc
     2821 gccgtcgtca gcacgatctc gaagatggtc gagctgcgcg acccgtacac gaccggtcac
     2881 gagcggcgcg tcggcgacct cgcgatcgcg atcggcaccg tgctcggctt cgacgccgac
     2941 cgcctcgacg gcctgcgcat cggcggttac ctgcacgacg tcggcaagat cgcgatgccg
     3001 tcgtcgatcc tgtccaagcc cgggcggctg agcgacgtcg agctcgcgct gatccgcgcg
     3061 cacgcgcaga tcggccacga ggtgctgtcg ggcatcgact tcccctggcc gatcgccgag
     3121 atgacgtggc agcaccacga gcggctcgac ggctccggtt atccgcgcgg cctgcgtggc
     3181 gacgagatcc tgctcgaagc ccgcatactc gcggtggccg atacagtgga agcgatgacc
     3241 tcgcaccgcc cgtaccggcc ggcggtcggg ctggcgctgg cgctcgaaga gatcgagcgc
     3301 ggccgcggcg tgctctacga ccccgaggtc gtcgatgcct gccttcagct gttccgcgag
     3361 gggcgtttcg ccttcgactg acgccgcccg acgcaggtcg ggcacggcag cttccgatcg
     3421 tcctgcaagg ggcggcgctg atcgcgggct ggacttcacc ggcccgtggc gttcaactgt
     3481 ctcccggcac gagttccgca gggaggcagc acatgagcgt caagtcgatg gcggcaatcg
     3541 tggcggccgt ggcgatgacc gtggtcgcgg cccgtgacct gcggccggca gaactggccc
     3601 gcagcccctg gctgcgcaac gcggtgcccg tccacgaagc cctgccgccc ggtcatccgc
     3661 cgatccccgg ccagcgcctg cctcagggcc atccgccggt cgagcgcgcc tatcccgacc
     3721 tgccgccggg ccatcccccg atcccgtggg cgcagcccgg ctgtcccgga caattcggtt
     3781 tcccggacga agggatcggc gtcggcttcg gccgcgacga gcgggcggtg atcagcacct
     3841 gaagaagtga ctagtggtca gtggctagtg gctagtggcg aaggtctggt gttgtggttg
     3901 cgctgtctgg taagaagcca gcgcccccgc cgagtccgat cctcgtacta gccactaacc
     3961 actagccact catgctttct ccgcgcatcg cgcggctcgg ccatcgctgg ctcgcgctgc
     4021 tggtcggctg gcaactcgcg atctggttcc tgagcggcct gtacatggtc gtcgtggacc
     4081 tcgacttcat ccacggcgac tcgctcgtgc gcaacgtcgc gccgccggtg cggctcgacg
     4141 aggccatcgc cccgttggca gcgatccgtg ccgggcgcga cgacgtccag gcgatccgca
     4201 tgcgcgtgct gcccgaccgc gatcagccgg tgtacgaggt ggtccggacc tcgggcaccg
     4261 aactgttcga cgccgggacg gggcggccga tgccggtcct cgacgaagcc cggattcgcg
     4321 cactcgcgca ggcctactac gcgggccggg gttcgattgc cggtgcgcag ttgctgacga
     4381 acgccgcgga cctgcccggc gagatccgcg ggcgccgccc cccggtctgg caaatcgatt
     4441 tcgacgactg gacggccacg tcgctgtacg tcgacccggt cacgggccgg ctggtgacgc
     4501 ggcgccaccg gttctggcgc tggttcgact tcctctggtc gctccacatc atggattacg
     4561 tcgagcgcga ggacgtcaac aacccgctgc tgcgcattgc gactccgctg gcgttcgtca
     4621 ccgtcgcgtt cggcggctgg ctggccttcc acagcttcgg attcctgcag cgtcgccgga
     4681 tgcgccgacg aggcgccgct tgaagctgct gcggctcagg aaactgcacc gctgggtcgc
     4741 gctggtcgtc ggcctgcagg tggtcgtctg gacggcgagc ggattcgcgt ttgcgtggct
     4801 cgaccaccac gacgtgaccg gcgaggcact ggccgcggcg cccgcgccgc gatcgctgcc
     4861 cgccgacgtc accgtgctcg acccggcacg gctgcccatc gcgcaggcga cgaccacggt
     4921 cgcgtcgctg gaactgcgcg acctcgacgg cacctgggtc taccgaatcg agacggtcga
     4981 cggacgcgtg tcgttgctgc gggcgagcga cggcgagccg ttcgcgatcg acgccgcgac
     5041 gacgcggcgc ctcgccgggc gtcattaccg cgggacgggt cggttgcagg aggtgcgtgc
     5101 ccagccggcc gggacgggcg agacgcgtgg cctcggggcc acgtggcagg ccacgtacga
     5161 cgatgccctg ggcacgagcc tgcacttctc cgccgccgac ggcgcgctgg tggccgtgcg
     5221 caccgacgcg tggcgcctca aggatttctt ctggatgctg cacacgatgg actatcgcgg
     5281 ccgcgacgat ttcaacaacc cgctggtcgt gacggccggc gcggcggccg cgtgggtcgg
     5341 gctgaccggg atctggctgc tgctgcgcgt tttccggcga cccgccgagg cggatgggga
     5401 ccggctcaga acttgaagat cttctcgagt tcgtccctga tcttgccttc gagcggctgc
     5461 ttgatggcgt ccttggccgc ggcctcgagc agcttcgaga cgtcgggacg gactttcggc
     5521 tccgccagcg tgcccgtgac gtcgagtggg atgtcggcgt ccttgacgcg gctcaccttg
     5581 atgccgagca gccggccctt tggcgccttg agcaggcgag ccgtcaggtc gaggtcgagc
     5641 gcgttacgac cataatcgac ttcgccgcga ccgcgtacgc gggcgaagtc cgcaacgaac
     5701 tcgacgcgat cgttgcgcag cgaccgctcg atgagcgtgc cgcgcgcaga cagtcgttcg
     5761 aagggcgtgc ggcccgaacc gcggccggtc gcggcagctg cctcgagctg cgcggcggcc
     5821 acggcacgtt cgagctcgtg ccagagatcg acgccgagca cggcaccgtc cgtgacctcg
     5881 acgtcgaacg gcccgcgcag gttcgcgaac aggctggcgc cgtcggcgcc gcgcgacgtc
     5941 agcgcgaaac ggccgctgcc gcggccctcg agttgcctga cgtcgaacaa ctgcccgagc
     6001 agggccgcga cgtcgacgcc cgcgagttcc tgccgcacgc tcaacgtcgg ttctggtgcc
     6061 gcgtagtcgt agcgcaggtg ggtcacgagc gagccgccga acgcgcgggc ctgcagcggc
     6121 tcgagctcga
//
LOCUS       DASPFR010000276         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_78479, whole genome shotgun sequence.
ACCESSION   DASPFR010000276 DASPFR010000000
VERSION     DASPFR010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78479"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..2039)
                     /locus_tag="VD737_07355"
     CDS             complement(183..2039)
                     /locus_tag="VD737_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXW10414.1"
                     /translation="MSRSPRACSWSIAAASCVACLAISQARAQAPEPTAQSEWRDVGR
                     VVAFADVHGAYDELTTLLREAGIVDTQGRWSAGRTHVVSLGDLLDRGAGSRQVMDLLM
                     RLQAEARAAGGALHVVLGNHEAMNVLGDLRYVDPGEYAAYADMESAEERARRRAEWER
                     TNGPGSGAAFDQKFPPGYFGHRAALAPDGPYGRWLLGLPVAVIVNDSLFMHAGPSAAI
                     AGMSVSDLNTRYRTALADYVRLYGQLERAGLLQAGDAFDARPRLAAERLAARGKDAGS
                     SSDLAAAVQAFERADAAPLLNPDGPNWYRGAALCNEVAETDVLAPLLRQFDVARVVIG
                     HTPTRDLRAVTRFDGRVVKLDTGMNRAVYKGRGAALTIDGPTLSVRYVGEPQPVAVRS
                     EGLYVAPNAVEDAAVAAALQAGTVSVTGPRAPGELAVVVEHQGRSIPAVFQQRTAGDA
                     RREVAAFRLDRHLGLGIVPATVEREVQGQRGVLQARPVKWVTQADVQKQSLRGGGWCA
                     TDPQFQLVYAFDGLIGNEARTLDTLLYDAGEWYVYSTGHARAFGHGREFPAYLKAQAP
                     RPGAELRRRLQRLDEATLASVLGEGIDARARKALLARRDALLALPAANAAGK"
BASE COUNT          322 a          793 c          772 g          336 t
ORIGIN      
        1 ttgccggccg caaatgcggc cggaaagtag tggtcagtgg ccagtggcta gtggtcagtg
       61 gctagtggct agtggctagt ggcgaggaga gcagaaggcc gacgcaatcg ccggccttct
      121 ttcttactga ccactgacca ctgaccactg gccactggcc actggccact agccactgac
      181 cactactttc cggccgcatt tgcggccggc aacgccaaca gcgcgtcgcg tcgtgcgagc
      241 aaggccttgc gggcacgcgc gtcgatgccc tctccgagga cggacgcgag cgttgcctcg
      301 tcgagccgct gcaggcgtcg gcgcagctcg gcgcccggtc tcggcgcctg cgccttcagg
      361 tacgccggaa attcgcgacc gtggccgaac gcgcgagcgt gtccggtcga gtagacgtac
      421 cactcgccgg cgtcgtagag cagcgtgtcg agcgtacggg cctcgttgcc gatcaggcca
      481 tcgaatgcgt agaccagctg gaactgggga tctgtggcgc accacccgcc gccccgcagc
      541 gactgtttct gcacgtccgc ctgcgtcacc cacttcacgg gacgcgcctg cagcaccccg
      601 cgctgtccct gcacctcgcg ctcgacggtg gccggcacga tgccgagtcc gaggtgccga
      661 tccaggcgaa aggcggcgac ctccctgcgc gcgtcgccgg ccgtgcgctg ctggaacacg
      721 gcgggaatcg accgcccctg gtgctcgacg accactgcga gttcgccggg ggcgcgcgga
      781 cccgtgacgc tcaccgtgcc agcctgcagc gcggcggcca cggctgcgtc ctcgaccgcg
      841 ttgggtgcca cgtacaggcc ctcggaacgc acggcgaccg gctgcggttc gccgacgtac
      901 cgcacgctca gcgtgggccc atcgatcgtc agggcggcac cgcggccctt gtaaaccgcc
      961 cggttcatgc cggtgtcgag cttgacgacg cgaccgtcga agcgcgtcac ggcgcgcagg
     1021 tcccgcgtcg gcgtgtgacc tatcactacg cgcgccacgt cgaactgccg cagcaatggc
     1081 gcgaggacat ccgtctccgc gacctcgttg cagagggcgg cgccccggta ccagttcggt
     1141 ccgtcgggat tcagcaatgg cgcagcgtcg gcccgttcga aggcctgcac ggccgcggcg
     1201 aggtcggacg acgaccccgc gtccttccct cgcgccgcga ggcgctcggc cgcgaggcga
     1261 gggcgcgcgt cgaatgcgtc gccggcctgc agcaacccgg cccgttcaag ctgcccgtac
     1321 agtcgcacgt agtcggcgag cgccgtccgg tagcgtgtgt tcaggtcgga cacgctcatg
     1381 ccggcgatcg cggcggaggg accggcgtgc atgaacagcg aatcgttgac gatgacggcg
     1441 acgggcagcc cgagcagcca gcgcccatac gggccgtccg gcgccagcgc ggcgcggtgg
     1501 ccgaaatatc cgggcggaaa tttctggtcg aacgcggcgc cggatcccgg cccgttcgtc
     1561 ctttcccatt cagcgcggcg acgtgccctt tcctcggcgg attccatgtc cgcgtaggcc
     1621 gcgtattcgc cgggatcgac gtagcgcagg tcgccgagga cgttcatggc ctcgtggttg
     1681 ccgagcacga cgtgcagcgc gccacccgcc gcacgggcct cggcctgcag ccgcatcagc
     1741 aagtccatga cctggcgcga accggcgccg cggtcgagca ggtcgcccag gctcaccacg
     1801 tgcgtgcgtc cggcggacca gcgaccctgg gtgtcgacga tccccgcttc gcgcagcagc
     1861 gtcgtcagtt cgtcgtaggc gccgtgcacg tccgcaaacg cgacgacgcg gccgacgtcg
     1921 cgccactcgg actgggccgt tggctcgggc gcctgggccc gggcctgcga tatcgccaga
     1981 cacgcgacgc acgacgctgc cgcgatggac caggagcagg cacgtgggct gcgtgacatg
     2041 aggttccctc gaggttcagt tcgcgggcgg cgcgacggtc gtgatctcgc cgctgtcgac
     2101 gcgctcgagc agccgcgcca gcaggcgctg gatcgactgg atctcgacgt tggtcaatcc
     2161 catctgcagc gtctcgccct tgcggcggac gccgcgtcgc ggcgccgtgt tctcgccaag
     2221 ggg
//
LOCUS       DASPFR010000277        12493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_322685, whole genome shotgun sequence.
ACCESSION   DASPFR010000277 DASPFR010000000
VERSION     DASPFR010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12493
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322685"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VD737_07360"
     CDS             complement(<1..761)
                     /locus_tag="VD737_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXW10415.1"
                     /translation="MSGDATPPVASKPGAREDGPADVTSPERMRRARRIGFTTIVTRE
                     FGRIVRIWAQTIVPPAITATLYFIIFGSLIGQRIGQMGGFTYMQYIAPGLIMMSVITN
                     SYGNVVSSFFGAKFGRHIEELLVSPLPNWLIVLGYVTGGVVRGLLVGLVVTLIALFFT
                     HLHVEHLATVLGAVLLTSTVFALGGFINAVFAKNFDQISFVPTFILTPMTYLGGVFYS
                     ITMLPDWAQQASKANPILYMVNAFRYGFLGQSDVDV"
     gene            complement(758..1714)
                     /locus_tag="VD737_07365"
     CDS             complement(758..1714)
                     /locus_tag="VD737_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXW10416.1"
                     /translation="MKALSLRQLTKTYRNGVQALKGIDLDVEDGDFFALLGPNGAGKS
                     TTIGIVSSLINKTAGTVTVFGRDIDTELEAAKSCLGVVPQEINFNQFEKVSTILLNQA
                     GFYGIPRSAARSRVEHWLRQLQLWDKRDAISRTLSGGMKRRLMIARALVHEPQLLILD
                     EPTAGVDIEVRRSMWEFLRRINSQGTTIILTTHYLEEAESLCRHIAIIDEGRIIENDR
                     MSTVLRKLHTETFVLNLREPLQLLPALPGYRVARIDETTLEIEVTKEQNLNAIFGALS
                     AAGIEVLSMRNKANRLEELFLRLVEGRDVGSGLARTSAGATG"
     gene            complement(1771..2460)
                     /locus_tag="VD737_07370"
     CDS             complement(1771..2460)
                     /locus_tag="VD737_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10417.1"
                     /translation="MTESYRAAPEARLHQGNLLNPAVRREIAGLNWLFLERAIRLRQA
                     GDPWFGVPREARDRLAAASLDAWERAAACPVALFELRLPDPGVEIASSDGVGEDAGAP
                     DDALVAETRRAFGITALGVARSLCDVVPLATRIVFGIDPRLEAQLCAMTLSDSYRLAA
                     WPGLIRPRWSGHERFWSLFAGAVTRAESVHWAYTAGLCLLAHCERQPTTVVYSARTRA
                     RPVHRRPDVPC"
     gene            2712..3128
                     /locus_tag="VD737_07375"
     CDS             2712..3128
                     /locus_tag="VD737_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10418.1"
                     /translation="MYRDYFGTNASNTVRRHRGKSPRQMSFFRRSLEHEQEAATLGSL
                     LLQCGLVRRHPNGFRPDIVAVAQFCDVYETLTSLFPKLHVSFEHAWHLWQVLCREDEY
                     VLSNCPGCQALWVRDTLEILPDNCPTCRRGLFRDVL"
     gene            3268..4227
                     /gene="nudC"
                     /locus_tag="VD737_07380"
     CDS             3268..4227
                     /gene="nudC"
                     /locus_tag="VD737_07380"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HXW10419.1"
                     /translation="MPRPPNVFAGPYLDRVAHLRKDEAWLAAALAADEAAIVPVWQSR
                     NLVEVRPEGLAAALLSAADFAAGERAGSFVLLGRFESRTYFAVDVDEAERVLSRFPDS
                     DVRFEDLRLVGGQLAAQQAGLLAYARGMLYWRQRHRFCGVCGAPTASTSAGHVMTCTN
                     RDCATDQFPRLDPAIIVLVTDGERALLGRQAAWPEGRYSTIAGFVEPGESLEDAVARE
                     VLEETGVRIDAADYHSSQPWPFPSSLMIGFTATADGAQVPKADEELEDVRWFTRAEIA
                     AGFPGLPPPQSVSYRLIEDWYDAGSTTPLRQTPGVRLWGSRRR"
     gene            4256..5026
                     /locus_tag="VD737_07385"
     CDS             4256..5026
                     /locus_tag="VD737_07385"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HXW10420.1"
                     /translation="MTLEIAFVLVAAFGAGFIDAMAGGGGLVQLPALFAAFPAAPHTT
                     LLGTGKLAGLAGTTSAIARFVRHVRLDWTLVLPAAAGAFVAALAGAWIATVFSPERFR
                     TLVPLLLTAILLYTLLHRDLGTEHRPRRRDRHGRWLAAGSAGAIGLYDGFFGPGTGSF
                     LVFLFVRVFGLDFLHASASAKLVNASTNVAALLLFGLTGELYWLLGFAMAACNVVGAQ
                     FGSHLAIRRGSSLVRRVFLVVVGSLIAKTAWDALRLHA"
     gene            5101..5712
                     /locus_tag="VD737_07390"
     CDS             5101..5712
                     /locus_tag="VD737_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HXW10421.1"
                     /translation="MDASWSTFAGIALGIGLAAATGFRVFLPLLIAGLAAHFGQLPLS
                     EHFQWLGSTPALIMLGTAAALEVVAYYVPGVDHALDFIAGPAALIAGVIASAAVMTDL
                     PPGVLWPVAIIGGGGIAGLAKGSAAMVRAKSGVMTGGLANPVVSTAETVGATGLALFA
                     VLLPVVTLVVVVVLFVWVVRRVRRRFFARGSGEGVTVPAEPKR"
     gene            5778..6626
                     /locus_tag="VD737_07395"
     CDS             5778..6626
                     /locus_tag="VD737_07395"
                     /inference="COORDINATES: protein motif:HMM:NF017551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HXW10422.1"
                     /translation="MCLLVLAWNVHPRYRLVVAANRDEFHDRPAAPLDWWRDVPGVLA
                     GRDLRGAGTWMGVSRSGRFGVVTNYRDLEPAPAPDAPSRGELVPKFLVDDAAPRGWLE
                     ALRSRAGRYAGFNLLLATDEELFYFSNRNGLEPQPLSPGLYGLSNHLLDSPWPKLLRA
                     RERLRELVTAGPLEAEALFALLADRTPADADAMPATGLPPDWERALSAPFVVHDRYGT
                     RCSTVLLVERHGGATTLERRFDASGIQTGASRWQFDVCPPSRTAASAAVPERAALREP
                     TAAWIE"
     gene            6623..8440
                     /locus_tag="VD737_07400"
     CDS             6623..8440
                     /locus_tag="VD737_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HXW10423.1"
                     /translation="MKPSRRVSSLHCFRLVRLLCLLFAVGFAGAAPAAPRLKERIELD
                     IVGVPDEIAENVRAYLTLTRYSTREDLTDPQVRRLADRAVDEATDALRPFGYYSPRVR
                     SRTSRDEPNWIVRLRIDPGQPVRMNTVEVLIEGPGAKDRELAAIVANSTLKPQTRLDH
                     TAYEALKASLMRTARDRGYLDATLTRRELIVNPTELTADARLTLETGGRYEFGALDID
                     QDVINDDLLRGFVRFQPGHPYSPSEITNTQYALDDSSYFSSVVVAPGEKDPETLTVPV
                     RIYGEPIKRDRYGINFGYGTDTGVRGQVTWDNRRVNKRGHRMRAELTASELRYEAAGR
                     YVIPVGDPSLEKLEFSVAYIDEKIGDLDSNRFEVTGGLTQVFGRWQQVLFLRLNRETT
                     GFPDGTEDDALLLIPGVSYASLPPNFLTGWVRDAAYYVELSGSPQSLGSDASYLRFYG
                     RAERVWKIDGPWYLRTRGELGTSWIDEFSELPASQRFFAGGDRSVRGFGINELSPPVD
                     PTKTSGGTSGSAENGGGGEHKLVGSLELERDFPRNFRGAVFFDFGNAFNDWNTPLEYS
                     VGLGVRWKLPMLMIGLDVAQALSESGMKPRFHLNITQVL"
     gene            8442..12251
                     /locus_tag="VD737_07405"
     CDS             8442..12251
                     /locus_tag="VD737_07405"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HXW10424.1"
                     /translation="MRPRRIAIVLGLVTLLLIAVAAVLAHRLLYTQAGLEFALSQLHR
                     VPGTRIEVTGARGTLAGPLDVSRLVVDHEALHLEARDVHVELQSNTLLAGKVRLRGLS
                     IGRVALRLKDRPERAEKRPGFLPAGLSIEAPSFEVGAAEVTLQGGRRIVADSARGSLE
                     LTRWRLIVDPLDVRSPDGAIAGSLALRATEPLGLRTNLRGEWRFPDDAFEYRFRAETR
                     GRLDRLAAELYLDSPARLSFSGTLLDLTGNPRARGTLRIAEFDGSPWLPPQKLPQLSG
                     TIALAAGRNGLGFDGTLTSPALPGQPLRLQGSGRFNERTVEVASLRAWLPRTGMELTS
                     SGRVELAPAGAPEGTLPRLALSGEWTALRWPLDERAKPVVTSPRGVYTLEGSMPYAFF
                     ARAEVEGAALPPVSFEASGSLDREAVELTRFDGYALRGRIGAQGRLQWTGPQPWRFDV
                     DARSLAIHELRPGVSGTVSARGSIAGAGLTATAPWTARLASLSGTMFGRPLTGHGEIA
                     HRDGTFELRDVRIANGPSFADVRGRVGRDSLDVDWNVDLRSLAIVTPGMSGQLVSRGR
                     ARGSLRRPQVTATARAERFEYQGVTADGITADVDLDSSDRQPSRVEIVANYVATGLLD
                     FDSVRFGLDGRMQEHRLALEFASPGNPERRIAAFQGLVGADGGLDVESRTWAGALTRA
                     GVVFAESDEATLIQPASISLSPQAQDVEPICLRTADDARLCVEGEHRSGPQRTWRVIY
                     SLQDWPLQRLLRTMLGWQEFDGRLQASGWAQQEPGKPWVGGTTVLVHEPAFEMRRNKF
                     RTERIQLGSSRVDLFVEPGEMRAELDLVVDESTGLRGEVHADRRANLLESPVRGQVVG
                     RSEAIKVLPLLVPEIDRAAGRLNGQVSVSGTLGAPAFNGDFQLRDGRLDLYRTNLTVT
                     ALQADGSFAGDVLRFQASGQTAKGALDVDGRFNWPGGVLTGTMRLRGDQLLVADTPEF
                     RVLASPDIVLRAGAEGYRVEGQVLIPTARISPREITSTVETSPDERIVGVPDIEDGAP
                     STSRRITSSIRVELGDAVRVEAYGLKARLDGDVTVATVPDDVARGTGTIRVAEGEYKA
                     FGQDVTITRGLLVYRNTPLSDPQLDIVAERKIKDTDITVAVNVRGSIDNPYIAITSTP
                     SMSSNEALSYLLTGRSIDTLQSGEAANVNQAAESLAVSGGGLLLGGLGTRLGLDEVSV
                     ERTGEDDTSVVLGKALSPKLFVSYGISIAEAINTIKLRYTLNERWSVKAESGLEQSAD
                     FEYRIER"
BASE COUNT         1851 a         4349 c         4354 g         1939 t
ORIGIN      
        1 acgtcgacgt cggactggcc gaggaagccg tagcggaacg cgttgaccat gtacaggatc
       61 gggttcgcct tcgaagcctg ctgcgcccag tcgggcagca tggtgatcga gtagaacacc
      121 ccgccgaggt aggtcatcgg cgtgaggatg aaggtcggca cgaacgagat ctggtcgaag
      181 ttcttggcga acacggcgtt gatgaacccg ccgagcgcga acacggtcga ggtgagcagc
      241 acggcgccga gcacggtcgc caggtgctcg acgtgcaggt gcgtgaagaa cagcgcaatc
      301 aatgtgacga cgaggcccac cagcaggccg cgcacgacgc cgccagtgac gtagcccagc
      361 acgatcagcc agttcggcag cggcgagacc agcagctcct cgatgtgccg gccgaacttg
      421 gcgccgaaga acgacgagac gacgttgccg tagctgttcg tgatgacgga catcatgatc
      481 agccccggcg cgatgtactg catgtaggtg aagccgccca tctggccgat gcgctggccg
      541 atcaggctgc cgaagatgat gaagtacagc gtcgccgtga tggcaggcgg cacgatcgtc
      601 tgcgcccaga tccgcacgat gcgaccgaac tcgcgcgtca cgatcgtggt gaagccgatc
      661 cgtcgggcgc gccgcatccg ctcggggctg gtcacgtccg cgggcccgtc ctccctggcg
      721 ccgggcttcg aggcgaccgg cggggtcgcg tcgccgctca tccggtggct cccgcggagg
      781 ttcgcgccag accgcttccg acgtcacggc cttccacgag gcgcaggaac agctcctcga
      841 ggcggttggc cttgttgcgc atgctcagca cctcgatgcc cgccgccgac agcgcgccga
      901 agatggcgtt gaggttctgc tccttggtga cctcgatctc gagcgtggtc tcgtcgatgc
      961 gcgcgacgcg ataccccggc agggcgggca gcagctgcag cggctcgcgc aggttgagca
     1021 cgaacgtctc ggtgtgcagc ttgcgcagca cggtcgacat gcggtcgttc tcgatgatcc
     1081 ggccctcgtc gatgatcgcg atgtggcggc agaggctctc ggcctcctcg aggtaatgcg
     1141 tcgtcaggat gatcgtcgtg ccctgggagt tgatccgccg cagaaactcc cacatcgagc
     1201 ggcgcacctc gatgtcgacg cctgccgtgg gctcgtcgag gatcagcagc tgcggctcgt
     1261 gcaccagtgc gcgcgcgatc atcaagcgtc gcttcatgcc gcccgacagc gtgcgcgaga
     1321 tcgcgtcgcg cttgtcccag agctgcagct gccgcagcca gtgctcgacc cggctgcgcg
     1381 ccgcggatcg cgggatgccg tagaagccgg cctggttgag caggatcgtc gacaccttct
     1441 cgaactggtt gaagttgatc tcctgcggca cgacgccgag gcacgacttg gcggcctcca
     1501 gttcggtatc gatgtcgcgg ccgaacacgg tcaccgtgcc ggccgtcttg ttgatcagcg
     1561 acgagacgat cccgatggtc gtcgacttgc cggcgccgtt cggcccgagc agcgcgaaga
     1621 agtcgccgtc ctcgacgtcc aggtcgatgc ccttcagcgc ctgcacgccg ttgcggtacg
     1681 tcttggtcag ctggcgcagc gacagggcct tcatgaacga cgacgaccgg gtgggcgggg
     1741 ggccgcaaag ggtaatgggg cctccccgcg tcagcaagga acgtcggggc ggcggtgtac
     1801 cggcctggcc cgggtccgcg cgctgtagac cacggtcgtg ggctgccgct cgcagtgcgc
     1861 aagcaggcac agcccggccg tgtatgccca atgcaccgac tccgcgcgag tgacggcccc
     1921 ggcgaacaac gaccagaacc gctcgtggcc ggaccagcgg gggcggatca gtccgggcca
     1981 ggccgccagg cggtacgagt ccgacagggt catcgcgcag agctgagcct ccagccgcgg
     2041 atcgatgccg aacacgatgc gcgtcgccag cggcacgacg tcgcagaggc ttcgcgccac
     2101 gccgagtgcc gtgatgccga atgcgcgccg cgtctccgcg acgagggcgt catccggggc
     2161 gccggcgtcc tcgcccacgc cgtcggatga cgcgatctcc actcccgggt ccggaaggcg
     2221 cagttcgaac aaggcgaccg ggcacgccgc ggcccgctcc caggcgtcca gcgacgccgc
     2281 ggccaggcga tcacgcgcct cgcgtgggac gccaaaccac ggatcgccgg cctgccggag
     2341 acggatcgcc cgctccagga acagccaatt gaggccggcg atttcgcgtc ggacggcggg
     2401 attgagcagg ttgccctggt gcagccgggc ctcgggcgcg gccctgtagc tctcggtcat
     2461 cgtgatccct ctttacatat atggtccaga atgaccatat atgtttatag actctcatat
     2521 atggtccgtt tagaccatat atgcacaaaa cgtccagttt cgggcgacgg gggaggcgca
     2581 tgcggatcac cgacagccgt tatgaccggg atcggctccg gctcaccctc gcctaccggc
     2641 tgatcggcct tgaggcccgc acccgcacca tccgcgaagc gacccaattg tcggatgacc
     2701 gaatccgtcg gatgtaccgt gattatttcg gcaccaacgc atcgaacacc gtgcgccgcc
     2761 accgcggcaa gtcccctcgg cagatgtcgt tctttcggcg ttcgctggaa cacgaacagg
     2821 aggccgcgac cctgggatcg ctgctgctcc agtgcgggct ggtgcgccgc cacccaaacg
     2881 gcttccggcc ggacatcgtc gcggtcgcgc agttctgtga cgtctacgag acgctcacga
     2941 gcctgtttcc aaaactgcac gtgagtttcg aacacgcgtg gcacctgtgg caggtgctgt
     3001 gccgcgagga cgagtacgtg ctgtcgaact gcccggggtg ccaggcgctc tgggtgcgcg
     3061 acacgctcga gatcctgccc gacaactgcc cgacctgccg tcgcgggctg ttcagggacg
     3121 tgctctgaac atccgcgcct gatgagcgca ctcagttccg gcggggtgcc agacgccgac
     3181 gctcggaggc tctgccggga tcgggttgtt cggcgccact tttgccgccg accgctaaac
     3241 tgcgactttc gattcgcaca cgtcgccgtg ccccgcccac caaacgtctt cgccggccct
     3301 tacctcgacc gcgtcgcgca cctgcgcaag gacgaggcct ggctcgccgc ggcgctggcg
     3361 gccgacgagg cggcgatcgt gccggtctgg cagtcccgga acctcgtgga ggtgcgcccc
     3421 gaaggcctcg cggcagcgct gttgagcgcg gcggatttcg cggccggcga acgcgcgggc
     3481 agcttcgttc tgctcggccg cttcgaatcg cgcacgtact tcgcggtcga cgtggacgag
     3541 gcggagcgcg tgctgtcgcg cttcccggac tcggacgtcc gattcgagga cctgcgtctc
     3601 gtcggcgggc aactcgccgc ccagcaggcc gggctgctcg cctatgcccg cggaatgctt
     3661 tactggcgcc agcggcaccg cttctgtggc gtctgcggag cccccaccgc cagcaccagc
     3721 gccgggcacg tcatgacgtg caccaaccgc gactgcgcga ccgaccagtt tccgcgtctc
     3781 gacccggcga tcatcgtgct ggtgaccgat ggcgagcgcg cgctgctcgg tcggcaggcc
     3841 gcgtggcccg aggggcgcta ctcgaccatc gcaggtttcg tcgagcctgg cgagagcctc
     3901 gaggacgcgg tcgcgcgcga ggtgctcgag gaaacgggcg tgcgcatcga cgcggccgac
     3961 taccactctt cgcagccgtg gccgttcccg tcgtcgctga tgatcgggtt cacggcgacc
     4021 gccgacggcg cgcaggtgcc gaaggcggac gaggaactgg aagacgtgcg gtggttcacc
     4081 cgcgccgaga tcgcggcggg gtttcccggc ctgccgccgc cgcagtcggt gtcctatcga
     4141 ttgatcgagg actggtacga cgccgggtcc accacgccgc tgcgccagac gccgggtgtc
     4201 cggctctggg gctcgcggcg ccgttgacga tgccgcggtc gcgactcgcc ctgtcgtgac
     4261 gctcgaaatc gcgttcgtgc tggtcgcggc gttcggcgcg gggttcatcg acgccatggc
     4321 cggcggcggc gggctcgtgc agttgcccgc gctgttcgcg gcgtttccgg cggcaccgca
     4381 caccacgctg ctcggcacgg gcaagctcgc ggggctggcc ggcacgacca gcgcgatcgc
     4441 gcgtttcgtc cgtcacgtac ggctggactg gacgctggtg ctgcccgccg cggccggcgc
     4501 tttcgtcgcc gcgctcgcgg gcgcgtggat cgccaccgtc ttctcgcccg agcgtttccg
     4561 gacgctcgtg ccgctgctgc tcaccgccat cctgctgtac acgctgctgc accgcgacct
     4621 gggcaccgag caccgtccgc ggcgccgtga ccggcatggg cgctggcttg ccgccggcag
     4681 cgcgggcgcg atcggtctct acgatggctt cttcggcccc ggtaccggca gctttctcgt
     4741 gttcctgttc gtgcgcgtct tcgggctcga cttcctgcac gcctcggcgt ccgcgaaact
     4801 cgtgaacgcg tccaccaacg tcgcggcgct gctgctgttc ggcctcaccg gcgagctgta
     4861 ctggctgctc gggttcgcga tggcggcctg caacgtcgtc ggtgcgcagt tcggcagtca
     4921 tcttgcgatc cgccggggca gctcgctggt gcgccgcgtc ttcctggtcg tcgtcgggtc
     4981 actgatcgcc aagaccgcgt gggacgcgct ccgactgcac gcttgaccga ccaccgacac
     5041 ccggtggacg cgaccgctag aatgcgcctg gacgcctggg caccgaggat cgaacccgac
     5101 atggacgcct cctggagcac gttcgccggc atcgcgctcg gcatcggcct cgccgcggcg
     5161 acgggattcc gcgtgttcct gccgctgctg atcgccgggc tcgcggcgca tttcggccag
     5221 ctgccgctgt ccgagcactt ccagtggctc ggcagcacgc ccgcgctgat catgctcggc
     5281 acggccgccg cgctcgaggt cgtcgcgtac tacgtgcccg gcgtcgatca cgcgctcgac
     5341 ttcatcgccg ggcccgccgc gctcatcgcc ggggtgatcg cgagtgccgc ggtcatgacc
     5401 gatctgccgc cgggcgtact ctggccggtg gcgatcattg gcggcggcgg catcgcgggc
     5461 ctggccaagg gcagcgccgc gatggtccgc gccaagtccg gcgtcatgac cggcgggctc
     5521 gcgaaccccg tggtgtcgac cgcagagacg gttggagcca cgggactggc gctgttcgcg
     5581 gtcctgctgc cagtcgtcac cctcgtggtc gtcgtggtgc tgttcgtctg ggtcgttcga
     5641 cgggtgcgcc gacgattctt cgcacgaggg tcgggcgagg gagtcacggt tccggctgag
     5701 ccgaagcgct gaacgaccgt cgacgcgcgc cgggcagtcg gccgccgcgg cgtgtccggc
     5761 cctacaatcc acgccctatg tgcctgctcg tgctcgcctg gaacgtgcat ccgcgctatc
     5821 ggctcgtggt cgccgcgaac cgcgacgaat ttcacgaccg ccccgcggcg ccgctcgact
     5881 ggtggcgaga cgtgcccggc gtcctggccg gtcgcgacct gcgcggggcc ggcacctgga
     5941 tgggtgtgtc ccgctctggc cggttcggcg tcgtgaccaa ctaccgtgac ctcgagccgg
     6001 caccggcgcc cgacgcgccg tcgcgcggcg agctcgtgcc gaaattcctc gtcgacgatg
     6061 cggcccccag gggctggctc gaagcgctgc gcagtcgcgc cgggcgctac gccgggttca
     6121 acctgctgct cgctacagac gaggaactgt tctatttttc caaccgcaac ggcctcgagc
     6181 cgcagcccct gtcgcccgga ctgtacggac tcagcaacca cctgctcgac tcgccctggc
     6241 cgaagctgct gcgcgcgcgc gagcggctgc gcgagctcgt caccgccggg ccgctcgaag
     6301 ccgaagcgct gtttgcgctg ctggccgacc gcactccggc cgatgccgac gcgatgccgg
     6361 cgacgggcct gccgcccgac tgggagcgcg ccttgtcggc gccattcgtc gtgcacgacc
     6421 gctacggcac acgttgctcg acggtgctgc tggtcgagcg gcacgggggt gcgaccacgc
     6481 tcgagcgccg tttcgacgcg agcgggatac agacgggggc gagccggtgg caattcgacg
     6541 tgtgcccgcc gtcgcgcacg gctgcgtccg ccgccgtccc ggaacgggcg gcgctgcgcg
     6601 agccgaccgc cgcgtggatc gaatgaagcc ctcgcgccgc gtgtcgtccc tgcactgctt
     6661 ccgtctcgtt cgcctgcttt gcctgctttt cgcggtggga ttcgcgggcg cggcacctgc
     6721 cgcaccgcgc ctgaaggagc ggatcgaact cgacatcgtc ggcgtcccgg acgagatcgc
     6781 ggagaacgtg cgcgcgtatc tcacgctgac gcggtactcg acgcgggaag acctgacgga
     6841 tccacaggtg cgccggctgg ccgaccgagc cgtcgacgaa gcgaccgacg cgctccggcc
     6901 gttcggctac tactcgccgc gcgtgcgcag tcgcacgtcg cgcgacgagc cgaactggat
     6961 cgtgcggctg cgcatcgacc caggccagcc cgtgcgcatg aataccgtgg aggtcctgat
     7021 cgaagggccg ggcgccaaag accgtgagct ggctgcgatc gtcgccaatt cgacgctcaa
     7081 gccgcagacg cggctcgacc acacggcgta cgaagccctc aaggcttcgc tgatgcgtac
     7141 ggcccgtgac cgcggctacc tggatgcgac cctcacgcgg cgcgagttga tcgtgaaccc
     7201 gacggagctg acggcggacg cgcggctgac gctcgagacc ggcggtcgct acgaattcgg
     7261 cgcgctcgac atcgaccagg acgtcatcaa cgacgatctc ctgcgcggtt tcgtgcgttt
     7321 ccagccgggc catccgtatt ccccgagcga gatcaccaac acgcagtacg cgctcgacga
     7381 cagcagctat ttctcgtccg tcgtcgtcgc gccgggcgag aaggatccgg agaccctgac
     7441 cgtgcccgtg cggatctacg gcgagccgat caagcgggac cggtacggca tcaacttcgg
     7501 gtacggcacc gacaccggcg ttcgcggcca ggtgacctgg gacaaccgcc gcgtcaacaa
     7561 gcgcggtcat cgcatgcggg ccgagctgac cgcgtccgaa ttgcgctacg aagccgccgg
     7621 ccggtacgtg atcccggtcg gcgatcccag cctcgagaag ctcgagttct ccgtcgccta
     7681 catcgacgag aagatcggcg atctcgacag caaccgcttc gaggtcaccg gcggcctgac
     7741 gcaggtgttc ggacgctggc agcaggtcct gttcctgcga ctcaaccgtg aaaccacggg
     7801 cttcccggac gggacggaag acgacgcgct gctgctgatt cccggcgtca gctacgcgag
     7861 cctgccgccc aacttcctga cgggatgggt gcgcgacgcc gcctattacg tcgaactctc
     7921 cggcagcccg cagtcgctcg gctcggacgc ctcgtacctg cgcttctatg ggcgcgccga
     7981 gcgcgtctgg aagatcgacg gaccctggta cctgcgcacg cgcggcgaac tcggcaccag
     8041 ctggatcgac gagttctccg agctgccggc gtcgcagcgc ttcttcgccg gtggtgaccg
     8101 cagcgtgcgt ggcttcggca tcaacgagct gagcccgccg gtcgacccca cgaagacctc
     8161 cggcggaacc agcggctcgg ccgaaaacgg cggcggcggc gagcacaagc tggtcggcag
     8221 cctcgagctc gagcgcgatt ttccacgcaa cttccgcggc gcggtcttct tcgacttcgg
     8281 caacgccttc aacgactgga acacgccgct cgagtactcg gtcggcctcg gcgtgcgctg
     8341 gaagctgccg atgctgatga tcggcctcga cgtggcgcag gcgctgtccg agagcggcat
     8401 gaagccgcgc ttccacctca acatcacgca ggtgctctag cgtgcggccg cggcggatcg
     8461 cgatcgtcct cgggctcgtc acgctgctgc tcattgccgt ggctgccgtg cttgcgcacc
     8521 ggctgctcta cacgcaggcc gggctcgagt tcgcactgag ccagctgcac cgcgtgccgg
     8581 gcaccaggat cgaagtgacc ggcgcccggg gcacgctcgc cggaccgctc gacgtgtcgc
     8641 gactggtcgt ggaccacgag gcgctgcacc tcgaagcgcg cgacgtgcac gtcgagctgc
     8701 agtcgaacac gctgctcgcc ggcaaggtgc gcctgcgcgg cctgtccatc ggccgcgtcg
     8761 cactgcgact caaggaccgg ccggagcggg ccgagaagcg accggggttc ctgccggcgg
     8821 gcctcagcat cgaggcgcca tccttcgagg tgggcgccgc cgaggtcacg ctgcagggcg
     8881 ggcgcaggat cgtcgccgac tcggctcgcg gctctctcga gctcacgcgc tggcggctga
     8941 tcgtcgaccc gctggacgtg cgcagccccg acggcgcgat cgccggatcg ctcgcgctgc
     9001 gagcgaccga accgctcggc ctgcgcacga acctgcgcgg cgagtggcga ttccccgatg
     9061 acgcgttcga gtatcgcttc cgcgcggaga cgcggggccg gctcgaccgg ctggcggcgg
     9121 agctgtacct cgattcgccg gcccggctca gcttctcggg cacgttgctc gacctcaccg
     9181 ggaatccccg cgcgcgcggg acgctgcgga tcgccgagtt cgacggctcg ccgtggctgc
     9241 cgccgcagaa gctgccgcag ttgagcggca cgatcgcact ggcggcgggt cgcaacgggc
     9301 tcggattcga cggcacgctg acgtcgcccg cgctgcccgg ccagcccctg cgattgcagg
     9361 gcagcggccg gttcaatgaa cgcacggtcg aggtcgccag ccttcgcgcg tggctgccgc
     9421 ggacgggcat ggagctgacg tcgagcggcc gcgtcgaact cgcgccggcc ggagcgccgg
     9481 aaggcacgtt gccccggctc gcgctgtcgg gcgaatggac tgcgctgcgc tggccgctcg
     9541 atgagcgcgc gaagccggtc gtgaccagcc cgcgcggcgt ctacacgctg gaaggctcga
     9601 tgccgtacgc gttcttcgcg cgcgccgagg tcgaaggcgc cgcgctgccg ccggtcagct
     9661 tcgaggcgtc gggctcgctc gaccgggaag ccgtcgagct cacgcgcttc gacggttatg
     9721 cgctgcgggg tcgcatcggg gcccaggggc gcctgcaatg gaccggcccg cagccctggc
     9781 gcttcgacgt cgacgcacgc tcgctggcga tccacgagct gcggcccggc gtgagcggga
     9841 cggtcagcgc gcgaggcagc atcgcgggcg cgggcctcac ggccaccgcg ccctggaccg
     9901 cgcggctcgc gtcgctgtcg ggaaccatgt tcggccgccc actgacgggg cacggcgaga
     9961 tcgcgcatcg cgacggcacg ttcgagctgc gtgacgtgcg catcgcgaac gggccgtcgt
    10021 tcgcagacgt gcgcggccgg gtcggccggg actcgctcga cgtcgactgg aacgtcgacc
    10081 tgcgatcgct cgcgatcgtc acgccgggca tgagcgggca gctggtctcg cgcggccgcg
    10141 cgcggggctc gctgcgtcgg ccgcaggtca cggccaccgc acgcgcggag cgcttcgagt
    10201 accagggcgt caccgcggac ggcatcactg cggacgtcga cctcgacagc agcgaccggc
    10261 aaccttcacg tgtcgagatc gtcgccaact acgtcgcgac cggcctgctc gatttcgact
    10321 cggtacgctt cggtctcgac ggacgcatgc aggagcatcg gctcgcactc gagttcgcat
    10381 ccccgggcaa ccccgagcgc aggatcgccg cgttccaggg actggtcggc gccgacggcg
    10441 ggctcgacgt cgagtcgagg acctgggcgg gtgccctcac gcgcgccggg gtcgtgttcg
    10501 cggaaagtga cgaggcgacg ctgatccagc cggcgtcgat cagcctgagt ccgcaggcac
    10561 aggacgtcga gccgatctgc ctgcgcaccg cggacgacgc gcggctgtgc gtcgaaggcg
    10621 agcatcggtc gggtccgcaa cggacgtggc gcgtgatcta cagcctgcag gactggccgc
    10681 tccagcgact gctgcgcacg atgctcgggt ggcaggagtt cgacggccgt ctgcaggcga
    10741 gtggctgggc ccagcaggag ccgggcaagc cgtgggtcgg cgggaccacc gtgctcgtgc
    10801 acgagcccgc cttcgagatg aggcgcaaca agttccgcac cgagcgtatc cagctcggca
    10861 gcagccgcgt cgacctgttc gtcgagccgg gcgagatgcg cgcggagctc gacctggtcg
    10921 tggacgaatc gaccgggctc cgcggcgagg tccacgccga ccggcgcgcc aacctgctcg
    10981 agtcgcccgt gcgcggccag gtggtcggcc gctcggaggc gatcaaggtg ctgccgctgc
    11041 tggtgccgga gatcgaccgc gccgccggcc ggctgaacgg ccaggtctcc gtgagcggca
    11101 cgctcggcgc gcctgccttc aacggcgact tccagctgcg cgacggtcgg ctcgacctct
    11161 accgcaccaa cctgaccgtg acggcgctgc aggcggacgg ctcgttcgcg ggcgacgtgt
    11221 tgcgcttcca ggcttcgggc cagacggcca agggcgcact cgacgtcgac ggccgcttca
    11281 actggccggg cggagtattg acggggacga tgcgcctgcg cggcgaccag ctgctcgtcg
    11341 ccgacacgcc ggagtttcgc gtgctggcct cgccggacat cgtcctgcgt gcgggcgcgg
    11401 aaggctatcg ggtcgaaggc caggtgctga tcccgacggc gcggatctcg ccgcgcgaga
    11461 tcacgagcac ggtcgagacg tcacccgacg agcgcatcgt cggggtgccc gacatcgagg
    11521 acggagcccc gtcgacgtcc cggcgcatca cgtcgagcat cagggtcgaa ctcggcgacg
    11581 cggtgcgcgt ggaggcgtac ggcctcaagg cgcgcctcga cggcgacgtg acggtcgcga
    11641 ccgtgccgga cgacgtcgcg cgcggcactg gcacgatccg cgtcgccgaa ggcgagtaca
    11701 aggcgttcgg ccaggacgtc acgatcacga ggggcctgct cgtctaccgc aacaccccgc
    11761 tcagcgaccc gcagctcgac atcgtcgccg agcgcaagat caaggacacc gacatcacgg
    11821 tcgcggtcaa cgtccgcggc tcgatcgaca atccctacat cgcgatcacg tcgacgccgt
    11881 cgatgtcgag caacgaggcg ctgtcgtacc tgctgaccgg gcgctcgatc gacaccctgc
    11941 agtcgggcga ggccgccaac gtgaaccagg ccgccgagag cctcgccgtg agcggcggcg
    12001 gcctgctgct cggcggcctc ggcacgcgac tcgggctcga cgaagtgtcc gtcgagcgga
    12061 ccggcgagga cgacacctcc gtcgttctcg gcaaggcgct gtcgccgaag ctgttcgtga
    12121 gctacggcat ctcgatcgcc gaggcgatca acacgatcaa gctgcgctac acgctgaacg
    12181 agcgctggtc cgtcaaggcg gagtcgggcc tcgagcagag cgcggacttc gagtaccgga
    12241 tcgaacggtg atggagagtg gctagtggtt agtggccagt gaagaacaag aaggcagcgg
    12301 ataccggttg ctccgggttc ttccactagc cactagccac taaccactaa ccacttcttc
    12361 agtcgaaatc cgtcggaatc gcagggtgca accagtgctg gcgcatgcgc aggacctgcg
    12421 ccggcgtgca gcggccgagc gacaacggcg gcagcgcgga cggatcgaag aaggctacgg
    12481 cgtcggtctc gta
//
LOCUS       DASPFR010000278        12557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1054458, whole genome shotgun sequence.
ACCESSION   DASPFR010000278 DASPFR010000000
VERSION     DASPFR010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12557
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054458"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1478
                     /locus_tag="VD737_07410"
     CDS             <1..1478
                     /locus_tag="VD737_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HXW10425.1"
                     /translation="HPDRRFEAMHEPVLGVIVDPVLARCGAWYEFFPRSASDDLQRHG
                     TFRDCEARLPYIEAMGFDVVYLPPIHPIGRVRRKGPNNTLTPGPEDPGSPWAIGADEG
                     GHRALHPQLGTLEDFKRLIASARARRLEVAMDIAFQCAPDHPYVRAHPEWFRFRPDGS
                     VQYAENPPKKYQDIYPFDFETEAWQPLHDELLGVVLYWAEQGVRVFRVDNPHTKPFAL
                     WESLIAEVKRRHPEAIFLSEAFTRPKVMHRLAKLGFTQSYTYFTWRNTRHELREYFTE
                     LSLSPSREYFRPNVWPNTPDILPEYLQVGGRAAFMTRVVLAATLSANYGIYGPAFELM
                     EHVPREPGSEEYRDSEKYQLRRWDLDRSDSLAPFIRRLNEIRRAHRALQFDRSLRWLE
                     IDNDQMIAYAKVLEDSDDAIVVVANLDPYHVHTAWLEMPLEELRIDPGQAYRMDDLLG
                     GGSFLWEGRRNFVKLDPNGVVAHVFVVRRRVRTEEQFDYFM"
     gene            1561..4857
                     /gene="treS"
                     /locus_tag="VD737_07415"
     CDS             1561..4857
                     /gene="treS"
                     /locus_tag="VD737_07415"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HXW10426.1"
                     /translation="MNEEQASTETTADPLWYKDAVIYQLHVKAFYDANGDGIGDFAGL
                     EQKLDYIQELGVNTLWLLPFYPSPQRDDGYDIADYRDVHPDYGTLDDFRRFIGAAHRR
                     GLRVITELVINHTSDQHPWFQAARRAPKGSPERDFYVWSDDETKFAGTRIIFTDTEAS
                     NWAWDPVAGQYYWHRFFSHQPDLNHNNPAVVDAVIDVMRFWLDMGVDGMRLDAIPYLC
                     VREGTNNENLPETHAVLRRMRAVVDAHYSGRLFLAEANQWPEDVREYFGEGDECHMAY
                     HFPLMPRMFMAVALEDRYPIAEILRQTPDIPENCQWAIFLRNHDELTLEMVTDKERDY
                     MYRMYAHEPRMRVNVGIRRRLAPLLGNDTDRIKLMNAMLLSMPGSPILYYGDEIGQGD
                     NIYVGDRNGVRTPMQWNVDRNAGFSRADPQRLYLPLIMDPVYGYQAVNVEAQSRDASS
                     LLNWTRRVLAVRSQFRSFGRGTLEFVRPANRKVLAYVRTFQGESVLCVFNLAQTAQSA
                     ELDLSAWKGRVPVELMGRNSFPPIGDLPYFVTLPVHSFFWLLLSDTADPPAWHVERQP
                     ATEVPILVLPAGLATLTEERGADRTARRTVQQLCAEVLPEFLRERRWFAGKGSTIRDV
                     QLAERALWRTPAGSWLISLIEVALADGLRQRYFLPLGLDPDDAGSGNVIRSSEWLLAR
                     IRESARSGVLVDAFADPAFCLQFVQAIESSSVIDFADGQIRFTPTRAFRDLASPGLEP
                     VVHLGREQSNTSVILGESLFLKAYRQARVGVNPDMEMPRFLTNAGFAAIPALAGAIEY
                     TRPDSPPITLAAVFAFARNQGDGWSYALNDLERLGAHVATEPDGVDQGTSPLFISQMR
                     TLGRRVGEMHAVLAADTDDEAFRPEPLGADDVPRLAQTVIAEAEMTLDLLERRFGELP
                     EGVQAGARAVLDARSDVLARVRDLSGAVRGAVRTRYHGDLHLGQVLLTADDFLITDFE
                     GEPARPIEQRRQKGSPLRDVAGMLRSFDYARAVGAERALTRSPTAAERVDAAFRAWRD
                     ASCDAFLEGYDLGVGKARSVPSDARDRARAIELFKVEKALYELRYELDNRPAWLGVPI
                     GGLLELLGRK"
     gene            complement(4880..6118)
                     /locus_tag="VD737_07420"
     CDS             complement(4880..6118)
                     /locus_tag="VD737_07420"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HXW10427.1"
                     /translation="MNNDTTLIELAPGVARPNPLGTVARRLVLSRMARLERGTLRIHE
                     GDEVHSFTGAAAGAAATLEVRDPAFYAELAYGGSVGAAESYMLGHWSTPDLTGLLRLL
                     LVNRDALDSLEGGLARLSAPLRLLAHRLHANTRAGSRRNISAHYDLGNDFFELMLDET
                     MMYSCALFERPEATLAEASRAKLDLVCRKLGLGPDHHVLEIGTGWGGFALHAASRFGC
                     RVTTTTISRSQHERARERVRAAGLDDRITVLLQDYRDLRGRYDRLVSVEMIEAIGHRQ
                     FETFFRRCGDLLASDGRMLLQSITIADRHYERARDEVDFIKRYIFPGCCIPSVGALTN
                     AMVDASDLRLVHLEDIGPHYATTLAAWRRNFHANLDRVRALGYPDTFVRMWEFYLCYC
                     EAGFAERALGDVQMVLTREG"
     gene            complement(6115..6897)
                     /locus_tag="VD737_07425"
     CDS             complement(6115..6897)
                     /locus_tag="VD737_07425"
                     /inference="COORDINATES: protein motif:HMM:NF018770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HXW10428.1"
                     /translation="MTTLYSALYRGRLEHRRHRPVRHAFGYPVCYAWLDLAELDRVFA
                     GRWFWSTRRPALAWWRRADYLGDPATPLDEAVRARVAAETGRRPAGPIRLLTLLRTFG
                     HSFNPVSFYYCYDVDDTRIDAVVAEITNTPWGERHAYVLPASAATRDDVGLRFRFGKR
                     FHVSPFMPMEQQYDWTLGEPGSALRIRMVNRAMSGERVFDASLALERRDVTGTNLARA
                     LARHPVASLDALRQIYWQALRLWLKRVPFHDHPGPRVEESTT"
     gene            complement(6857..8125)
                     /locus_tag="VD737_07430"
     CDS             complement(6857..8125)
                     /locus_tag="VD737_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXW10429.1"
                     /translation="MKIAIVGAGIAGNVAARELHREHEITVFESGDHVGGHSHTHEIA
                     ADGRTWQVDTGFIVFNDRTYPHFTALLESLGVPAQESSMSFSVRDELADLEYNGTSLN
                     SLFAQRRNLLRPSFLGMVRDILRFNREAPRLLDEPGGERPLGELLAQGRYGRAFVDHY
                     VVPMGAAIWSTDPGSMMQFPARFFVRFLHNHGMLSVDDRPTWRTVRGGSARYVERLVA
                     PFRERIHLGTPVEQVRRIPGGVLVKTRGHEAQRYDAVFLACHSDQALAMLGDPSPAER
                     DVLAAIPYQANDAVLHTDARLMPRRRLAWAAWNYHVRPEGGPVALTYNMNILQRLDAP
                     QPFLVTLNHTDAIDPDRILRRMTYHHPLYTPASVAAQARHREIDGAQATYYCGAWLRN
                     GFHEDGVASALAAVQHFRDDLVQRALPRSA"
     gene            complement(8122..9066)
                     /locus_tag="VD737_07435"
     CDS             complement(8122..9066)
                     /locus_tag="VD737_07435"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HXW10430.1"
                     /translation="MSSRLTVALRRWVDSGVAGDEATARPRGIDWPRVVPFLLLHVAC
                     FGVLWTGWSPFAVGVAVTLYAVRMFAITGFYHRYFSHRAFRATRGWQFVFAVLGASAV
                     QRGPLWWAAHHRHHHANSDRPGDAHSARQDGFLWSHMGWFLARENFATRAHLVRDLAR
                     YPELRWLDRYDVVVPAALFAALWGLGAWLESAHPSLGTGPWQLVVWGFCISTVVLFHA
                     TFVINSLAHGWGSRRYDTGDDSRNNLLLALVTFGEGWHNNHHRYPSSARQGFYWWEID
                     LSWYVLRLLAALGIVRDLRPVSSSVLAAGRRAEPERVS"
     gene            complement(9063..9611)
                     /locus_tag="VD737_07440"
     CDS             complement(9063..9611)
                     /locus_tag="VD737_07440"
                     /inference="COORDINATES: protein motif:HMM:NF019819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HXW10431.1"
                     /translation="MKHTRLLASLAALGVAGCSSVPHAPVPLVDHVDLPRFMGDWYVI
                     ASIPTYFERDAWNAVEAYRLDPDGSIATTFTYNKGGFDGPERRMTPRGFVVDRTSNAV
                     WGMQFVWPIKADYRISYLSEDYGQTVIAREKRDYVWIMARTPTLSDTDYARLTSFVAS
                     IGYDTSKLRKVPQRWPAAEDTP"
     gene            complement(9622..10269)
                     /locus_tag="VD737_07445"
     CDS             complement(9622..10269)
                     /locus_tag="VD737_07445"
                     /inference="COORDINATES: protein motif:HMM:NF040005.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6134 family protein"
                     /protein_id="HXW10432.1"
                     /translation="MKRTAAAVMASLAVAASASTATSRAPDTGREWHFRVLLDDQEVG
                     WHRYVVRTDDEATEVESRAQFDVRVLFVNAYRYRHEARERWQGACLEGLASRTVTNGQ
                     VEDVDAVATDEALVVARRSGETRLPGCVMSFAYWDPRILQADRLLNPQTGEWLPVRIE
                     TRGTEQVSVSGRTVTATRHRLSAPEMQIDLWYADGLWVGLEAAMRGGRLLRYELQ"
     gene            complement(10335..11375)
                     /locus_tag="VD737_07450"
     CDS             complement(10335..11375)
                     /locus_tag="VD737_07450"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HXW10433.1"
                     /translation="MTQGTHRAIGWVEQGYVPDSVVRAGIRRLCEQRLREIEAGDCER
                     GAAATEAFVTAMDGAELAPLPHLANAQHYEVPARFFELALGPHLKYSSAWWPTGTRTL
                     ADAERRSLATTCERAGLADGQQILELGCGWGSLTLWMAEHYPGSRITAVSNSASQRAW
                     IEAQARRRGLDNVTVVTADMNVFAAPGQYDRIVSVEMFEHMRNWRELFGRVHDWLVPG
                     GRFFMHVFCHRSTPYAFVDGGPADWMSRHFFSGGMMPSDDLALRFQDRLRLVARWRWD
                     GTHYERTANAWLRNVDARRDEVLDVLAATHGEAAPQWLQRWRVFFMACAELFGYRNGQ
                     EWWVGHYLFARP"
     gene            complement(11599..12099)
                     /locus_tag="VD737_07455"
     CDS             complement(11599..12099)
                     /locus_tag="VD737_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10434.1"
                     /translation="MPKFNRQRLVFGLYLLALIAVAELAAGYLGVPAWPAFMAMIFFF
                     VEHMNPKKAPEILVGGVAGIACILLAEPVIGALTPLVGFESARLVYILGLVYAIVAFG
                     EMLPLVFNNYAFMYLTVTGLAVHLPEPQPLLWMAVAGLGGGLLIAGVLGIVRFMSAGA
                     AASQPG"
     gene            12291..>12557
                     /locus_tag="VD737_07460"
     CDS             12291..>12557
                     /locus_tag="VD737_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXW10435.1"
                     /translation="MADFPWTQSYPAGVRWDAELPLMPVQQILDEAVARWPERPALDF
                     MGRVISYRELGELADRAAAGLQRLGVGPGVHVGLYLPNSPHYPIA"
BASE COUNT         2017 a         4350 c         4328 g         1862 t
ORIGIN      
        1 gtcacccgga ccggcgcttc gaggccatgc acgagccagt cctcggtgtc atcgtcgatc
       61 ccgtgctggc ccgctgcggc gcctggtatg aattcttccc gcgttcggcc agcgacgacc
      121 tccagcgcca cggcacgttc cgcgactgcg aagcgcgcct gccgtacatc gaggcgatgg
      181 ggttcgacgt cgtctacctg ccgcccatac atccgatcgg ccgggtccgt cgcaagggcc
      241 cgaacaacac gctgacgccg gggcccgagg atcccggcag cccgtgggcg atcggtgccg
      301 acgagggcgg ccaccgtgcg ctgcacccgc agctcggaac gctcgaggac ttcaagcggc
      361 tgatcgcgag cgcgcgtgcc aggcgcctcg aggtcgccat ggacatcgcc ttccagtgcg
      421 cgccggacca cccgtacgtg cgggcccacc cggagtggtt ccgcttcagg cccgacggct
      481 cggtccagta cgccgagaac ccgccgaaga aataccagga catctacccg ttcgacttcg
      541 agaccgaggc ctggcagccg ctgcacgacg agttgctcgg cgtggtcctc tactgggccg
      601 aacagggcgt ccgcgtgttt cgcgtcgaca atccgcacac caagcccttc gcgctgtggg
      661 agagcctgat cgcggaggtc aagcggcgcc atccggaggc gatcttcctg tccgaggcgt
      721 tcacgcggcc gaaggtcatg caccgtctcg cgaagctcgg cttcacccag tcgtacacgt
      781 atttcacctg gcgcaacact cgacacgagc tgcgcgaata cttcaccgag ttgtccctga
      841 gcccgtcccg tgagtacttc cggcccaacg tctggcccaa cacgccggac atcctgcccg
      901 agtacctgca ggtcggcggg cgcgcggcgt tcatgactcg ggtcgtcctc gcggccaccc
      961 tgagcgcgaa ctacggcatc tacgggccgg cgttcgagct gatggaacac gtgccgcgcg
     1021 agcccggcag cgaagagtac cgtgactcgg aaaagtacca gctgcgccga tgggacctcg
     1081 atcgcagcga cagcctggcg ccgttcatcc ggcgcctgaa cgagatccgt cgcgcgcacc
     1141 gggcgctgca gttcgatcgc tcgctgcgct ggctcgagat cgacaacgac cagatgattg
     1201 cctacgccaa ggttctcgaa gactccgacg acgccatcgt cgtcgtcgcg aacctggacc
     1261 cctaccacgt gcacaccgcg tggctggaga tgccgctcga ggagctgcgc atcgaccccg
     1321 ggcaggccta ccgcatggac gacctgctcg gcggcggcag cttcctctgg gagggccggc
     1381 gcaatttcgt gaaactcgac ccgaacggcg tcgtggccca cgtcttcgtc gtcaggcgcc
     1441 gcgtgcgcac cgaggagcag ttcgactatt tcatgtaggg cggtccgcga ctcgagccgg
     1501 cgggccgcgg cgaggcagcc cgacgagtct gccgtgcccg tcgcgatgct ggagacctcg
     1561 atgaacgaag aacaagcgag cacggagacg accgccgatc cgctctggta caaggacgca
     1621 gtcatctacc agctgcacgt gaaggcgttc tacgacgcca acggcgacgg catcggtgat
     1681 ttcgccggac tggagcagaa gctcgactac atccaggaac tgggcgtcaa caccctgtgg
     1741 ttgctgccct tctacccctc gccgcagcgc gacgacggct acgacatcgc cgactaccgt
     1801 gacgtccatc ccgactacgg cacgctcgac gacttccggc gattcatcgg cgccgcccat
     1861 cgccgcgggc tgcgggtgat caccgagctc gtcatcaacc acacctccga ccagcacccc
     1921 tggttccagg cggcgcgacg cgcgccgaag gggtcgccgg agcgtgactt ctacgtgtgg
     1981 tccgacgacg agacgaagtt cgccggcacg cggatcatct tcacggacac cgaggcgtcg
     2041 aactgggcgt gggaccctgt tgccggccag tactactggc atcgcttctt ttcgcaccag
     2101 ccggacctca accacaacaa cccggccgtc gtcgacgcgg tgatcgacgt gatgcgcttc
     2161 tggctcgaca tgggcgtcga cgggatgcgc ctggacgcga ttccgtacct gtgcgtacgc
     2221 gaaggcacca acaacgagaa cctgccggag acgcacgccg tgctgcgcag gatgcgggcg
     2281 gtcgtcgacg cgcactacag cggccggctg ttcctcgccg aggccaacca gtggcccgaa
     2341 gacgtacgcg agtacttcgg cgagggcgac gaatgccaca tggcgtacca cttcccgctg
     2401 atgccgcgca tgttcatggc ggtggcgctc gaggaccggt atccgatcgc ggagatcctg
     2461 cggcagaccc ccgacatccc ggagaactgc cagtgggcca tcttcctgcg caatcacgac
     2521 gagctcacgc tcgagatggt gaccgacaag gagcgcgact acatgtaccg catgtacgcg
     2581 cacgagccgc gcatgcgcgt caacgtcggc atccgtcgcc gcctggcgcc gctgctgggc
     2641 aacgacaccg accgcatcaa gctcatgaac gcgatgctgc tgtcgatgcc gggctcgccg
     2701 atcctgtact acggcgacga gatcgggcag ggcgacaaca tctacgtcgg cgaccgcaac
     2761 ggcgtgcgca cgccgatgca gtggaacgtc gatcgcaacg ccggattttc ccgcgccgat
     2821 ccgcagcggc tgtacctgcc gctgatcatg gacccggtct acgggtacca ggccgtcaac
     2881 gtcgaagcgc agagccgcga cgcgtcctcg ctgctgaact ggacccggcg cgtgcttgcc
     2941 gtccgcagcc agttccgcag cttcggccgg ggcacgctcg agttcgtgcg tccggccaat
     3001 cgcaaggtgc tggcctacgt ccgcacgttc cagggcgagt ccgtgctctg cgtgttcaac
     3061 ctcgcccaga cggctcaatc ggcagagctg gatctttcgg cctggaaggg gcgggtgccc
     3121 gtcgaactga tgggtcgcaa ttcgtttccg ccgatcggcg acctgccgta cttcgtcacc
     3181 ctgccagtcc acagcttctt ctggctgctg ttgagcgaca cggcggaccc gcccgcctgg
     3241 cacgtcgagc gccagccggc gacggaggtg ccgatcctcg ttctgccggc cggcctggcg
     3301 acgctcaccg aagagcgggg cgccgatcgc accgcccgcc gcacggtcca gcagctgtgc
     3361 gccgaggtcc tgccggagtt cctgcgcgag cgacgctggt tcgccgggaa gggcagcacg
     3421 atcagggacg tgcagctggc cgagcgcgct ctgtggagga cgccggccgg cagctggctg
     3481 atcagcctga tcgaggtcgc actggccgac ggcctacggc agcgatactt cctgccgctc
     3541 ggcctcgatc cggacgacgc cggcagcggc aacgtcatcc gctcgagcga atggctcctg
     3601 gcccggatcc gcgagagcgc gcgttccggc gtgctggtcg atgcgtttgc ggaccccgcc
     3661 ttctgcctgc agttcgtgca ggcgatcgag agctcgtccg tgatcgactt tgcagacggc
     3721 cagatccgat tcaccccgac ccgcgcgttc cgggacctgg ccagccccgg gctggagccc
     3781 gtggtgcacc tcggccgcga acagagcaac accagcgtca tcctcggcga gagcctgttc
     3841 ctgaaggcct atcggcaggc gcgcgttggt gtgaatccgg acatggagat gccgcgcttc
     3901 ctgacgaacg cgggcttcgc cgcgatcccc gcactcgcgg gtgcgatcga atacacgcgg
     3961 cccgactcgc caccgatcac gctcgcggcc gtgttcgcgt ttgcgcgcaa ccagggcgac
     4021 ggctggtcgt acgcgttgaa cgacctcgag cgactgggcg cccacgtcgc gacggaaccg
     4081 gacggcgtcg accagggcac cagcccgctg ttcatctcgc agatgcggac gctgggccgg
     4141 cgcgtcggtg agatgcacgc cgtgctggcc gcggacacgg acgacgaggc cttcaggccc
     4201 gagccgctgg gcgcggacga cgtcccgcgc ctggcgcaga ccgtgatcgc ggaggccgag
     4261 atgacgctcg acctgctgga acgacgcttc ggcgagctgc ccgaaggggt gcaggccggc
     4321 gcacgcgcgg tgctcgacgc gcgcagcgac gtgctggccc gggttcggga cttgtcggga
     4381 gccgtgcgcg gcgcggtcag gacgcgctat cacggcgacc tgcatctcgg ccaggtgctg
     4441 ctgaccgccg acgatttcct gatcacggac ttcgagggtg agccggcgcg tccgatcgag
     4501 cagcgccgcc agaagggctc gccgctgcgc gacgtcgccg gcatgctgcg ttcattcgac
     4561 tacgcgcgtg ccgtcggggc cgagcgcgca ctgacgcgca gccccaccgc cgccgagcgc
     4621 gtcgacgccg ccttccgcgc ctggcgcgac gcgtcgtgcg acgccttcct ggagggctac
     4681 gatctcggcg tgggcaaggc ccgcagcgtg ccgtccgatg cccgtgaccg ggcgcgcgcc
     4741 atcgagctgt tcaaggtcga gaaagccctg tacgagctgc gctacgagct cgacaaccgc
     4801 ccggcgtggc tcggcgtgcc gatcggcggg ctgctcgaac tgctgggccg aaaatgaccc
     4861 tgccgcgcga ggtccccgct cagccctccc gcgtcagcac catctgcacg tcgccgagcg
     4921 cccgctcggc gaacccggcc tcgcagtagc acaggtagaa ctcccacatc ctgacgaacg
     4981 tgtccggata accgagcgcg cgcacccggt cgaggttcgc gtggaagttg cgacgccacg
     5041 cggcgagcgt ggtggcgtag tgcgggccga tgtcctccag gtgcaccagc cgcaggtcgc
     5101 tcgcgtcgac catggcgttc gtgagcgcgc cgaccgacgg gatgcagcag ccggggaaga
     5161 tgtagcgctt gatgaagtcg acctcgtcgc gcgcccgctc gtagtgccgg tccgcgatcg
     5221 tgatcgactg cagcagcatg cggccgtcgc tcgcgagcag gtcgccgcag cgccggaaga
     5281 aggtctcgaa ctggcggtgg ccgatcgcct cgatcatctc gaccgagacc aggcggtcgt
     5341 agcggccgcg caggtcgcgg tagtcctgca gcagcacggt gatgcggtcg tcgagcccgg
     5401 ccgcgcggac ccgctcgcgc gcccgttcgt gctggctccg cgagatcgtg gtggtcgtga
     5461 cgcggcagcc gaagcggctc gcggcgtgca gcgcgaagcc gccccagccg gtgccgatct
     5521 cgagcacgtg gtggtccggg ccgaggccga gcttgcggca cacgaggtcg agcttggcgc
     5581 gggaagcctc cgcgagcgtc gcctcgggac gctcgaacag cgcgcacgaa tacatcatcg
     5641 tttcgtcgag catcagctcg aagaagtcgt tgccgaggtc gtagtgggcg gagatgttcc
     5701 ggcggctgcc ggcacgcgtg ttggcatgca gccggtgcgc gagcaggcgc agtggcgccg
     5761 agagtcgcgc gagtccgcct tccagcgagt ccagcgcgtc gcgattgacg agcagcagtc
     5821 gcagcagtcc ggtcaggtcg ggcgtcgacc agtggcccag catgtacgat tcggcggcgc
     5881 cgaccgagcc gccataggcg agctcggcgt agaacgccgg atcacgcacc tccagggtgg
     5941 cggccgcacc ggccgccgcg ccagtgaatg agtgcacctc gtcaccctcg tggatgcgga
     6001 gcgttccccg ctcgagccga gccatgcgcg acagcaccag ccgtcgcgcc accgtgccga
     6061 gcggattggg ccttgcgaca cccggtgcaa gctcgatcag ggtcgtgtcg ttgttcatgt
     6121 cgtcgattcc tccacccgag gtcccgggtg gtcgtgaaag ggaacgcgct tgagccacag
     6181 gcgcagcgcc tgccagtaga tctgtcgcag cgcgtcgagg ctcgcgaccg ggtgccgggc
     6241 cagcgcccgg gcgaggttcg tgcccgtgac gtcgcgtcgc tccagcgcga gcgacgcgtc
     6301 gaagacccgt tcgccgctca tggcgcggtt caccatgcgg atccgcagcg ccgagccggg
     6361 ttcgccgagc gtccagtcgt actgctgctc catcggcatg aacggcgata cgtgaaagcg
     6421 cttgccgaag cgaaagcgca ggccgacgtc gtcccgggtc gccgcggacg ccggcagcac
     6481 gtaggcgtgt cgctcgcccc acggcgtgtt ggtgatctcg gcgaccaccg cgtcgatgcg
     6541 cgtgtcgtcc acgtcgtagc agtagtagaa gctcaccggg ttgaagctgt gcccgaacgt
     6601 gcgcagcagc gtgagcaggc ggatcggtcc ggccgggcgg cggcccgtct ccgcggccac
     6661 gcgggcgcgc acggcctcgt cgagcggcgt cgccgggtcg ccgaggtagt cggcacggcg
     6721 ccaccacgcg agcgcgggac ggcgcgtcga ccagaaccac cggccggcga acacgcgatc
     6781 gagctccgcg aggtcgagcc aggcatagca caccggatag ccgaacgcat gccgcaccgg
     6841 ccggtgccgg cggtgctcaa gccgaccgcg gtagagcgcg ctgtacaagg tcgtcacgga
     6901 agtgctgcac ggcggcgagc gcgctcgcga cgccgtcctc gtggaagccg ttgcgcagcc
     6961 aggcgccgca gtagtacgtc gcctgcgcac cgtcgatctc gcgatggcgg gcctgcgcgg
     7021 cgaccgaggc cggcgtgtag agcgggtggt ggtacgtcat gcggcgcagg atgcggtcag
     7081 ggtcgatcgc gtccgtgtgg ttcagcgtca ccaggaacgg ctgcggcgca tcgagccgct
     7141 gcaggatgtt catgttgtac gtgagcgcga ccggtccacc ctcgggtcgc acgtgatagt
     7201 tccacgcggc ccatgcgagg cgccggcgcg gcatcagccg tgcgtcggtg tgcagcacgg
     7261 catcgttggc ctggtacggg atcgcggcca gcacgtcccg ctcggccggg ctcgggtccc
     7321 cgagcatggc gagcgcctgg tcgctgtggc aggccaggaa caccgcgtcg tagcgctgcg
     7381 cctcgtggcc acgcgtcttg acgagcacgc cgcccgggat gcggcgcacc tgctccacgg
     7441 gcgtccccag gtggatgcgc tcccggaacg gcgccacgag tcgctccacg tagcgcgccg
     7501 aaccgccacg caccgtgcgc cacgtggggc gatcgtccac gctcagcatc ccgtggttgt
     7561 gcaggaatcg gacgaagaac cgggctggaa actgcatcat cgagcccggg tcggtcgacc
     7621 agatcgccgc gcccatcggc accacgtagt gatcgacgaa ggctcggccg taacggccct
     7681 gcgcgagcag ctcgccgagc ggccgctcgc cacccggctc gtcgagcagg cggggcgcct
     7741 cgcggttgaa gcgcaggatg tcgcgcacca tgccgaggaa cgacggccgc agcaggttcc
     7801 gtcgttgcgc gaacagcgaa ttgagcgagg tgccgttgta ctcgagatcc gcaagctcgt
     7861 cgcgcacgga gaagctcatg ctgctctcct gcgcgggcac gccgagcgat tcgagcagcg
     7921 ccgtgaagtg cggataggtc cggtcgttga agacgatgaa gccggtgtcg acctgccagg
     7981 tgcgcccgtc cgcggcgatc tcgtgcgtgt ggctgtggcc gccgacgtga tcgcccgact
     8041 cgaacacggt gatctcgtgc tcgcggtgca gttcgcgtgc ggcgacgttg ccggcgatgc
     8101 cggcaccgac gatggcgatc ttcacgacac gcgctccggc tccgcgcgcc gtccggccgc
     8161 cagcaccgac gacgacaccg gacgcaggtc gcgcacgatg ccgagcgcgg cgaggagccg
     8221 cagcacatac cagctcaggt cgatctccca ccagtagaac ccctggcggg cggagctcgg
     8281 atagcgatgg tggttgttgt gccacccctc gccgaacgtg accagcgcga gcagcaggtt
     8341 gttgcgcgaa tcgtcgccgg tgtcgtagcg gcggctgccc cagccgtgcg ccagcgagtt
     8401 gatcacgaag gtcgcatgga acagcacgac ggtcgagatg cagaaccccc agacgacgag
     8461 ctgccagggc cccgtgccga gcgacgggtg cgccgactcg agccaggcac ccagccccca
     8521 caatgccgcg aacagcgcgg ccggcacgac cacgtcgtag cggtcgagcc agcgcagctc
     8581 gggatagcgc gcgaggtcgc ggaccaggtg ggcgcgcgtg gcgaagttct cacgcgcgag
     8641 gaaccagccc atgtggctcc agaggaaccc gtcctggcgc gccgagtggg catcgcccgg
     8701 gcgatccgag ttcgcgtggt ggtgccggtg gtgggccgcc caccagagcg gtccgcgttg
     8761 cacggccgag gcaccgagca ccgcgaagac gaactgccag ccgcgcgtcg cccgaaacgc
     8821 gcggtgcgag aaataacggt ggtagaaccc ggtgatcgcg aacatgcgca cggcgtagag
     8881 cgtcacggcg acaccgacgg cgaacgggct ccagcccgtc cacagcacgc caaagcaggc
     8941 gacgtgcagc agcaggaacg gcacgacgcg cggccagtcg atgccgcgcg gccgggcagt
     9001 cgcctcgtcg cccgccacgc cgctgtcgac ccagcgccgc agcgcgacgg tgagacgcga
     9061 actcacggcg tgtcctcggc cgcgggccag cgctgcggga ctttccggag cttgctcgtg
     9121 tcgtagccga tcgaggcgac gaagctcgtg agtcgtgcgt agtcggtgtc gctcagcgtc
     9181 ggcgtgcgcg ccatgatcca gacgtagtcg cgcttttcgc gcgcgatcac cgtctggcca
     9241 tagtcttcgg acaggtagga gatgcggtag tcggccttga tcggccacac gaactgcatg
     9301 ccccacaccg cgttcgaagt gcgatcgacg acgaagccgc gcggcgtcat gcgacgctcg
     9361 ggaccgtcga agccaccctt gttgtacgtg aacgtcgtcg cgatgctgcc gtcggggtcg
     9421 agccggtacg cctcgaccgc gttccacgca tcgcgctcga aatacgtcgg gatgctggcg
     9481 atcacgtacc agtcgcccat gaagcgcggg aggtcgacgt ggtcgacgag cggcacgggc
     9541 gcgtgcggca cgctgctgca tccggcgacg ccgagcgcgg ccagtgaggc aaggaggcga
     9601 gtgtgcttca tggggtcgtg ctcactggag ttcgtaacgc aacaggcggc cgccgcgcat
     9661 cgcggcctcg aggcccaccc agagaccgtc ggcgtaccag aggtcgatct gcatctcggg
     9721 cgccgaaagc cggtgacgcg tggccgtgac ggtgcggccg ctcacgctca cctgttccgt
     9781 gccgcgggtc tcgatgcgca cgggcagcca ttcgccggtt tgcggattca gcagtcgatc
     9841 ggcctgcagg atgcggggat cccagtaggc aaagctcatg acgcagccgg gcaggcgcgt
     9901 ctcaccggag cggcgggcga cgaccagtgc ctcgtcggtg gcgaccgcgt cgacgtcctc
     9961 gacctgaccg ttggtcacgg tgcgcgacgc gagtccttcg aggcaggcgc cctgccagcg
    10021 ctcgcgcgcc tcgtggcgat atcgataggc gttgacgaac agcacccgca cgtcgaactg
    10081 cgcgcgactc tcgacctccg tggcttcgtc gtcggtgcgc acgacgtagc gatgccagcc
    10141 gacttcctgg tcgtcgagca gcacccggaa gtgccattcg cggccggtgt ccggggcccg
    10201 ggacgtggcg gtcgacgccg acgcggccac ggcgaggctg gccatgaccg ccgctgccgt
    10261 gcgcttcacg acggtgccgc ggatgcctgg gccacggcac gccgccccgc gccgccgcga
    10321 tttcatcgcc cgcatcacgg ccgcgcgaac aggtagtgac cgacccacca ctcctggcca
    10381 ttgcggtacc cgaagagctc ggcgcaggcc atgaagaaca cgcgccagcg ctgcagccac
    10441 tgcggcgccg cctcgccgtg cgtcgccgcg agcacgtcga ggacttcatc ccgcctcgcg
    10501 tcgacgttgc ggagccacgc gttggcagtg cgctcgtagt gcgtgccgtc ccagcgccag
    10561 cgggccacga gccgcaaacg gtcctggaaa cgcagtgcga ggtcgtcgct cggcatcatg
    10621 ccgcccgaga agaagtgacg gctcatccag tcggccgggc caccgtcgac gaaggcatac
    10681 ggcgtggagc ggtggcagaa cacgtgcatg aaaaagcgtc cgccgggtac gagccagtcg
    10741 tgcacgcgtc cgaacaactc acgccagttc cgcatgtgct cgaacatctc gaccgagacg
    10801 atgcggtcgt attgcccggg cgccgcgaac acgttcatgt ccgccgtcac cacggtgacg
    10861 ttgtcgaggc cgcggcgccg tgcctgggcc tcgatccacg cccgctgcga ggctgaattg
    10921 gaaaccgccg tgatgcggct gccggggtag tgctcggcca tccagagcgt gagcgagccc
    10981 cagccacaac cgagttcgag gatctgctgc ccgtcggcga gcccggcacg ctcgcaggtc
    11041 gtcgcgagcg accggcgctc cgcgtcggcg agcgtccttg tgccggtcgg ccaccaggcc
    11101 gaggagtact tgaggtgggg tccgagtgcg agttcgaaga accgcgcggg cacttcgtag
    11161 tgctgcgcgt tcgccaggtg cggcagcggc gcgagttcgg cgccgtccat ggccgtcacg
    11221 aaggcctcgg tggcggcggc cccacgttcg cagtcgccag cctcgatctc ccgcaggcgc
    11281 tgttcacaca gccggcgtat gccggcccgc acgacggagt cggggacgta gccctgttcc
    11341 acccaaccga tggctcggtg cgtgccttga gtcatgggat ccgtccccgt agaagttgta
    11401 catagcctat acaaagacct cctaatggtc aacaaatacc gtacataggc tggacaacgg
    11461 cgcgcgtgcc ggcctgccac ccggccgggg cggtcattca gtcgaggatg ggcggcgcag
    11521 gacgcacggc gccgcgccct gaggtcatcg cacggactgc gtcggccgct tccgccaggt
    11581 cgcgccgacg cgcgcgcctc accccggctg gctggccgcc gcccccgccg acatgaacct
    11641 gacgatgccg aggacccccg cgatcagcag tccgccgccg aggcctgcga ccgccatcca
    11701 cagcaggggc tggggttccg gcaggtgcac ggcgaggccg gtcaccgtca ggtacatgaa
    11761 cgcgtagttg ttgaagacca gcggcagcat ttcgccgaac gccacgatcg cgtagacgag
    11821 tccgaggatg tagacgagcc gggcagactc gaagcccacg agcggggtca gtgcgccgat
    11881 gaccggctcg gcgagcagga tgcacgcgat cccggcgacg ccgcccacca ggatctcggg
    11941 ggctttttta gggttcatgt gctccacgaa gaagaagatc atcgccatga acgccggcca
    12001 cgccggtact cccaggtaac ctgcggcgag ttcggccacg gcgatcagcg cgagcaggta
    12061 gaggccgaac accagtctct ggcggttgaa cttcggcatg cgcgtcgtcc tgtagatccc
    12121 ccgatcgccg gcgatcttag gcgcgccgcc aggcaccgca tataagtgac ttcgactcac
    12181 cggcgtcggc ggcgagcacg cgaatcctgc caagtactga ttgacgttcc gacgagcccc
    12241 ttcgacactc ccctgctacc gccgtcgctg cgccaatgcc ggagctggtc atggccgact
    12301 tcccctggac ccagtcatac cccgcgggcg ttcgctggga cgccgaactg ccgttgatgc
    12361 cggtgcagca gatcctcgac gaggccgtgg cgcgctggcc ggagcggccg gcgctggact
    12421 tcatgggccg cgtcatcagc tatcgggagc tgggcgagct ggcggatcgt gccgcagccg
    12481 ggctgcagcg gctgggcgtc gggccgggcg tgcacgtggg gctgtacctg ccgaactcgc
    12541 cgcactaccc gatcgcc
//
LOCUS       DASPFR010000279         2849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1420415, whole genome shotgun sequence.
ACCESSION   DASPFR010000279 DASPFR010000000
VERSION     DASPFR010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420415"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1689
                     /locus_tag="VD737_07465"
     CDS             <1..1689
                     /locus_tag="VD737_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HXW10436.1"
                     /translation="AGPARAVPAVDDATAIAAAVDVLRRGEVLAVKGVGGYHLLCDAR
                     SEAAVARLRERKRRPDKPLAVMFPWRGDDGLAAVNDEFDLEPAARAALLDASRPIVLL
                     SRTGRATALATGVAPGLHEVGVFLPYSPLHQLLLEAYGSPVVATSGNVSGEPVLTEPA
                     EAATRLATVADAFLHHDRPIVRPADDSVVRVIAGRARPLRLGRGVAPLELELPVAVPH
                     PVLAAGAHLKNTVALAWDRRVVVSPHIGDMGTLRSEIVFAQVAADLQRLYDVRAAAVL
                     CDAHPDYATSRWARASGLPVHPVLHHHAHASAVVQEHGAQAEDTIVFAWDGVGLGGDG
                     TLWGGETFVGRPGQWHRRASLRPFHLPGGDRAGRAPWRSAAALCWELGIECPVPVPDP
                     IVRSAWQRRLNAPRSSAAGRVFDAAASLILGVGETSFEGQGPMWLEAIARPAADGPVL
                     PLVPDGDLVIADWGPLVSWLVAHPDVEPGARAGAVHASLAQAIVAIAERLRREHGCAT
                     VGLTGGVFQNRLLSEQASRALDSCGFRVLLAVQLPCNDGGLSFGQVADFAGRKS"
     gene            1697..>2849
                     /locus_tag="VD737_07470"
     CDS             1697..>2849
                     /locus_tag="VD737_07470"
                     /inference="COORDINATES: protein motif:HMM:NF006385.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase family protein"
                     /protein_id="HXW10437.1"
                     /translation="MLWFERPFLAVLRHEYRASMRFTEDDTAEVMPAAPAFPCQLYVH
                     VPFCEVLCPFCSFHRVRYDAVRTRRYFDALRREIQLYHDAGFRFGDVYVGGGTPTVDA
                     DALIETLELVRRLSPLRTVSIETNPNHLDRDTLARYRDAGVTRLSVGVQSFDDGLLRG
                     MERLDKYGDGDLIRRRIAAVQGLFPTLNVDMIFNLPGQTLAMLDHDLDVLRGLAVDQI
                     SFYPLMTAPTARHKMEKSMGRNDPSLRHPMYERILERLLAEEYHAASAWCFSRNQGMF
                     DEYIIDQDDYVGVGSGAFSYVGGAMYSTTFSLNHYCRRVEGGQTGITQRRPVGTKERM
                     RYDYLVRLFGGELRRDYITRRYGRTFDLLLAPELLAMRLLGATRHDADAI"
BASE COUNT          381 a         1003 c         1019 g          446 t
ORIGIN      
        1 gcggggccgg cccgtgcggt gccggcggtc gacgacgcga cggccatcgc ggccgccgtc
       61 gacgtgctgc gtcgcggcga ggtgctggcg gtcaagggcg tcggcggcta tcacctgctg
      121 tgcgacgcgc gcagcgaggc ggccgtcgcg cgcctccgcg agcgcaagcg ccgaccggac
      181 aagccgctcg cggtgatgtt tccatggcgc ggcgacgacg gcctcgcggc cgtgaacgac
      241 gagttcgacc tcgaacctgc cgcgcgggct gcgctgctcg acgcgagccg gccgatcgtg
      301 ctgctgtcac ggaccggtcg ggccactgcg ctggcgaccg gcgtcgcgcc ggggttgcac
      361 gaggtcggcg tgttcctgcc ttacagcccg ctgcatcagt tgctgctcga ggcgtacgga
      421 agcccggtcg tcgcgacctc gggcaacgtg agcggcgagc cggtgctcac cgaaccggcg
      481 gaggcggcga cgcgcctcgc gacggtggcc gacgcgttcc tgcaccacga ccggccgatc
      541 gtgcgacccg ccgacgattc cgtggtgcgg gtgatcgccg ggcgtgcacg gccgctgcgc
      601 ctcgggcgtg gcgtggcgcc gctcgaactg gaactcccgg tcgcggtgcc gcacccggtg
      661 ctggccgccg gcgcgcacct caagaacacc gtcgcgctgg cgtgggacag gcgtgtcgtc
      721 gtctcgccgc acatcggcga catgggcacg ctgcgcagcg aaatcgtgtt cgcgcaggtc
      781 gccgcggacc tgcagcggct ctacgacgtg cgcgcggcgg ccgtgctctg cgacgcgcac
      841 cccgattacg ccacctcgcg ctgggcgcgc gcgagcggcc tgccggtgca cccggtcctg
      901 catcaccacg cccatgcctc cgcggtggtg caggaacacg gcgcacaggc cgaggacacg
      961 atcgtgttcg cctgggacgg cgtcggcctc ggcggcgacg gcacgctgtg gggcggcgag
     1021 acgttcgtcg gccgtcccgg ccagtggcat cgtcgcgcga gcctgcgccc gtttcacctg
     1081 ccgggcggtg atcgcgcggg ccgcgcaccg tggcgcagtg ccgcggcgct ctgctgggag
     1141 ctcgggatcg agtgcccggt gccggtgccg gacccgatcg tgcgctcggc ctggcagcgc
     1201 cggctcaacg cgccgcgctc ttcggccgca ggacgcgtgt tcgacgccgc ggcgtcgctg
     1261 atcctcggtg ttggagagac gagcttcgaa gggcagggac cgatgtggct cgaagcgatc
     1321 gcacgtcccg cggcggacgg cccggtcctg ccgctcgtgc cggacggcga cctggtgatc
     1381 gccgattggg gcccgctcgt gtcgtggctg gtcgcgcatc cggacgtcga gccgggcgcg
     1441 cgcgcggggg cggtgcacgc ttcgcttgcc caggcgatcg tggccatcgc ggagcgactg
     1501 cggcgcgagc acggctgcgc gaccgtcggc ctgaccgggg gcgtcttcca gaatcgcctg
     1561 ctgagcgagc aggcgtcgcg cgcgctcgac agctgtggct ttcgcgtact gctggccgta
     1621 caactgccct gcaatgatgg cggtctcagc ttcggccagg tggccgactt cgccggccgg
     1681 aaatcataga tcgcccatgc tctggttcga acgccccttc ctcgcggtcc tgcgtcacga
     1741 gtaccgcgcc tcgatgcgct tcaccgagga cgacacggcc gaggtcatgc cggcggcgcc
     1801 ggcatttccg tgccagctgt acgtgcacgt gccgttctgc gaggtgctgt gcccgttctg
     1861 ctcgttccat cgcgtgcggt acgacgcggt caggacacgc cggtacttcg acgcgctgcg
     1921 gcgcgagatc cagctgtacc acgacgccgg attccgcttc ggcgacgtgt acgtgggcgg
     1981 gggcacgccc accgtggacg cagacgcgct gatcgagacg ctcgaactcg tccgtcgctt
     2041 gtcgccgctg cgcacggtct cgatcgagac caacccgaac cacctcgacc gcgacacgct
     2101 cgctcgctac cgcgacgccg gggtcacgcg cctgtcggtc ggggtgcaga gtttcgacga
     2161 cgggctgctc aggggcatgg agcgcctcga caaatacggc gacggggacc tgatccgacg
     2221 ccgcatcgcc gcggtgcagg gactcttccc gacgttgaac gtcgacatga tcttcaacct
     2281 gcccggccag acgctcgcga tgctcgacca cgacctcgat gtgctgcgcg ggctcgcggt
     2341 cgaccagatc tcgttctatc cgctgatgac cgcgccgacc gcgcgtcaca agatggagaa
     2401 gagcatgggg cgcaacgacc cgtcgctgcg ccacccgatg tacgagcgga tcctcgagcg
     2461 gctcctggcc gaggaatacc acgcggcctc ggcctggtgc ttctcgcgca accagggcat
     2521 gttcgacgaa tacatcatcg accaggacga ttacgtcggc gtgggcagtg gcgccttcag
     2581 ctatgtcggc ggtgcaatgt attccacgac attctcgctg aatcactact gccgtcgcgt
     2641 cgaaggcggc cagaccggca tcacgcagcg acgcccggtc ggcacgaaag agcgcatgcg
     2701 ctatgactac ctggtgcggc tgtttggcgg cgagttgcgg cgcgactaca tcacgcgccg
     2761 ttacggccgt acgttcgacc tgttgctggc gcccgagctg ctggcgatgc gcctgctcgg
     2821 cgccacgcgt cacgacgccg atgccatcc
//
LOCUS       DASPFR010000280         3849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1420437, whole genome shotgun sequence.
ACCESSION   DASPFR010000280 DASPFR010000000
VERSION     DASPFR010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420437"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VD737_07475"
     CDS             complement(<1..443)
                     /locus_tag="VD737_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXW10438.1"
                     /translation="MHADANFWLILAGTLAVGWGVTSYRNERRRQRRHRRRARYLEGV
                     RFLLDDKPDRALEAFLTIAELDDETVDTHFALGSLYRRRGEVDRAIRVHQHIYDNASL
                     EDGHRDAALTELARDYFRAGLFDRAEKLFQQLADDGRDPALALQHL"
     gene            complement(433..756)
                     /locus_tag="VD737_07480"
     CDS             complement(433..756)
                     /locus_tag="VD737_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10439.1"
                     /translation="MLRRVAFGIVLAFLVVLAMLFVALNQQHFEVDVAFLEFDVSSGL
                     ALLIAFAAGLLAGAFARARWVAELLAERGRLRQALRLAETRLGTAVPAAKVATVPAPA
                     NADAR"
     gene            complement(917..1207)
                     /locus_tag="VD737_07485"
                     /pseudo
     CDS             complement(917..1207)
                     /locus_tag="VD737_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
     gene            complement(1289..3067)
                     /gene="rpsA"
                     /locus_tag="VD737_07490"
     CDS             complement(1289..3067)
                     /gene="rpsA"
                     /locus_tag="VD737_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016568693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HXW10440.1"
                     /translation="MPLTTGREAGRRFIHPTLGGQDAREEPGHEAHEKSMTESFAQMF
                     EESLASQRIRPGQILTGRVIEVGPDVVVVSAGLKSEAVIPVQQFKNERGEVEVAVGDD
                     VEVALDSVEDGSGETRLSREKAKRARTWERLETAFEKQEVVKGLINGRVKGGFTVEIE
                     FVRAFLPGSLVDVRPVRDPSYLEGKVHEFKIIKLDQKRNNVVVSRRAVVEQEYSAERS
                     ELLDKLQEGAVVKGVVKNLTDYGAFVDLGGIDGLLHITDMAWKRVKHPSEVVNVGDEV
                     DVRILKFDRERQRVSLGLKQLGADPWQNIARRYPSGTRLFGKVTNIADYGCFVEIEEG
                     VEGLVHVSEMDWTNKNVNPSKVVHIGQEVEVMVLDIDEERRRISLGIKQCQPNPWREF
                     ADNYNRGDRVSGQIRSITDFGLFIGLPGGIDGLVHLSDLSWDVAGEEAVRQYQKGQSV
                     EAVVVAIDPERERISLSVKQLAKDPFSTWIAEHPKGSIVRGLVREVDAKGAIVDLGGG
                     IDGYLRASELARDRVEDARQHLKVGDEVEAKFTAVDRKVRTVALSIKAKDMAEEQEAM
                     ESYRSDSTTGTSLGDLLKEQISGRDS"
     gene            complement(3101..3781)
                     /gene="cmk"
                     /locus_tag="VD737_07495"
     CDS             complement(3101..3781)
                     /gene="cmk"
                     /locus_tag="VD737_07495"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HXW10441.1"
                     /translation="MLPIITIDGPSGSGKGTIARRVAGSLGFHLLDSGALYRLVALAA
                     LRQGLAPDDEPGHARVARTLDVEFGVDEAGEERVLLVGEVVTPEIRTEAAGGEASRVA
                     AMPSVRAALLDRQRAFARPPGLVADGRDMGTVVFPDAPLKLYLTASPEERARRRHKQL
                     KEKGVTVTIARLSQEIRERDMRDSSRPVAPLRPAADAIQLDSTGLTIEQVVEQVLALA
                     RSRLPAMY"
BASE COUNT          592 a         1307 c         1284 g          666 t
ORIGIN      
        1 aggtgctgca gcgcgagtgc aggatcgcga ccgtcgtccg caagctgctg gaacagcttc
       61 tcggcgcggt cgaacaggcc ggcgcgaaag tagtcgcgcg cgagctccgt caacgctgcg
      121 tcacggtgac cgtcctcgag acttgcgttg tcgtagatgt gctgatgcac ccggatcgcg
      181 cggtccacct cgccccgcct gcgatagagg ctgccgagcg caaagtgcgt gtccacggtt
      241 tcgtcgtcga gttcggcgat cgtgaggaac gcctcgagcg cgcggtctgg cttgtcgtcg
      301 agcaggaagc gcacgccttc caggtatcgc gcgcgccggc ggtggcggcg ctggcgacgc
      361 ctctcgttgc ggtacgaggt gaccccccag ccgaccgcga gcgtccccgc caggatcagc
      421 cagaagttgg catcagcgtg catctgcgtt ggcaggcgca ggtaccgtcg cgaccttcgc
      481 ggccgggacg gccgtgccga gacgcgtctc ggccagtcgc agcgcctgtc gcaggcgacc
      541 tcgctccgcc agcagttcgg cgacccagcg cgcccgcgcg aacgcgccgg caagcaggcc
      601 cgccgcaaac gcaatcagca gcgcgagacc cgacgacacg tcgaactcca ggaacgcgac
      661 gtcgacctcg aagtgctgct ggttcaatgc cacgaacagc atggcgagga ccaccaggaa
      721 cgcgaggacg ataccaaagg cgacgcgacg cagcatgccg ctctcaccgg gaagtcagga
      781 aaacatgcac gcggagtcta gcatccgcgc gcgcgggcgc cggtcagtcg accgtgctcg
      841 ggctcgccga aggcagcggc cgcgaagacg acgtatcgtc gtcgtcgtca tcggcgtctt
      901 cggagccgtc gggctcgttg acgcgttcgc ggaggtcctt gcctggcttg aagtgcggca
      961 cgtacttgcc ggccagtgcg acggtctcgc cggtcttggg attgcggccc tgtcgcggcg
     1021 gccggaaatg caggctgaag ctgccgaagc cgcggatctc gatgcgctgt ccctgggcga
     1081 gcgcgtcgct catgatctcg aggatctgct ttacggccag ctcgacgtcc ttcgcgggca
     1141 ggtgactctg cttggcagcg atgagctcga tgagctcgga cttcgtcatg ctgcgctcgg
     1201 cgtccactcg aacccccggg atctcttgta gttcgtgcgc tggcgcggga cccgcggcgg
     1261 cgaggtcgcc accgcgggcc cgtgcggatc agctgtcgcg gccggagatc tgctccttga
     1321 gcaggtcgcc caggctcgta ccggtcgtcg agtcggagcg atagctctcc atcgcctcct
     1381 gctcttcggc catgtccttc gccttgatcg agagcgccac ggtgcggacc ttgcgatcca
     1441 cggccgtgaa cttggcctcg acctcgtcgc ccaccttcag gtgctggcgg gcgtcctcga
     1501 cacggtcacg cgcgagctcc gaagcgcgca ggtagccgtc gatgccgccg cccaggtcga
     1561 cgatcgcgcc cttggcgtcg acttcgcgga cgaggccgcg aacgatcgag cccttcgggt
     1621 gctcggcgat ccaggtgctg aacgggtcct tcgcgagctg cttgacgctg agcgagatgc
     1681 gctcgcgctc cgggtcgatc gcgacgacga cggcttcgac agactggccc ttctggtact
     1741 ggcgcaccgc ctcctcgccc gcgacgtccc aggagaggtc ggacaggtgc acgaggccgt
     1801 cgatgccgcc cggcagcccg atgaacaggc cgaagtcggt gatcgaacgg atctggccgc
     1861 tgacgcggtc gccgcggttg tagttgtccg cgaactcacg ccacggattc ggctggcact
     1921 gcttgatgcc gagcgatata cgacgacgct cttcgtcgat gtccaggacc atgacctcga
     1981 cttcctggcc gatgtgcacg accttggacg ggttgacgtt cttgttggtc cagtccatct
     2041 cggacacgtg gacgaggcct tcgacgccct cctcgatctc gacgaagcag ccgtagtcgg
     2101 cgatgttggt gaccttgccg aacaggcggg taccggacgg gtaccggcgc gcgatgttct
     2161 gccacgggtc cgcgccgagc tgcttgagtc cgaggctcac gcgctgccgc tcgcggtcga
     2221 acttgaggat gcgcacgtcg acctcgtcgc cgacattgac gacttcggac gggtgcttga
     2281 cgcgcttcca cgccatgtcg gtgatgtgca gcaggccgtc gatgccgccc aggtcgacga
     2341 acgcgccgta gtcggtgagg ttcttgacca cgcccttgac gaccgcgcct tcctgcagct
     2401 tgtcgagtag ctccgagcgc tcggcgctgt attcctgctc gacgaccgcg cgacgcgaca
     2461 cgaccacgtt gttgcgcttc tggtcgagct tgatgatctt gaactcgtgc accttgcctt
     2521 cgaggtagct cgggtcgcgc acgggccgca cgtccacgag cgaaccgggg aggaacgcgc
     2581 gcacgaactc gatctcgacc gtgaagccgc ccttgacgcg gccattgatg aggcccttga
     2641 cgacctcctg cttctcgaat gcggtttcca gtcgctccca cgtgcgggcc cgcttggcct
     2701 tttcacggga aagacgcgtc tcgccggagc cgtcctcgac gctgtccagc gcgacttcga
     2761 cgtcgtcgcc aacggcgact tcgacttcgc cgcgctcgtt cttgaactgc tggaccggaa
     2821 tgacggcttc ggacttgagt cctgcgctca cgacgaccac gtcgggaccg acttcgatca
     2881 cgcggccggt gaggatctgg cccgggcgaa tccgctggct cgccaggctt tcttcgaaca
     2941 tctgggcaaa actttcggtc atgcttttct cgtgggcctc gtggcccggt tcctcgcgag
     3001 cgtcctgccc gccaagggtt gggtggatga aacgacgacc ggcttccctg cccgtcgtca
     3061 acggcaaacg gcaccgcccc cgccatcggg cgcggcgccg tcaatacatc gcggggagcc
     3121 ggctccgggc cagggcgagg acctgttcca cgacctgctc gatggtgagc cccgtcgagt
     3181 cgagctgaat tgcgtccgcc gccgggcgca atggcgccac cggccggctg gaatcgcgca
     3241 tgtcccgctc gcggatctcc tgcgaaaggc gggcgatggt aaccgtaact cccttttcct
     3301 tcaactgctt atgacgtctt cgggcgcgtt cctcggggct cgccgtcagg tagagcttca
     3361 gtggcgcatc ggggaaaacc acggtgccca tgtcccgacc gtcggccacc agccccggtg
     3421 gccgggcaaa tgcgcgctgc cggtcgagca gcgccgcccg caccgacggc atggcagcga
     3481 ccctggaggc ctcgccaccg gccgcttcgg tgcggatttc cggggtgacg acctccccga
     3541 ccagcagcac gcgttcctcc ccggcctcgt cgacgccgaa ctcgacgtcc agcgtacgcg
     3601 ccaccctggc atggcccggc tcgtcgtcgg gcgccagccc ctggcggagc gctgccagcg
     3661 ccacgagccg gtagagcgcc ccgctgtcca gcaggtgaaa gcccagcgaa ccggcgaccc
     3721 gcctggcgat cgtgcccttg ccggagccgg agggaccgtc gatggtgatg atgggaagca
     3781 tgggaagtga ccgggaagtg accgggaagt gactagtgaa tagtgactgg tgactagtcg
     3841 gaacgcacg
//
LOCUS       DASPFR010000281         3489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1420543, whole genome shotgun sequence.
ACCESSION   DASPFR010000281 DASPFR010000000
VERSION     DASPFR010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1420543"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(895..971)
                     /locus_tag="VD737_07500"
     tRNA            complement(895..971)
                     /locus_tag="VD737_07500"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(935..937),aa:Pro,seq:ggg)
     gene            complement(1034..1390)
                     /locus_tag="VD737_07505"
     CDS             complement(1034..1390)
                     /locus_tag="VD737_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020190138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXW10442.1"
                     /translation="MLEPSNNDELPTIPGKRYFTIGEVSELCGVKPHVLRYWEQEFPQ
                     LKPVKRRGNRRYYQRQDVLVIRQIRALLYDQGFTIGGARNRLDGEEARVDVTQTHQLA
                     RQLRVELEELIKILRR"
     gene            complement(1371..1670)
                     /gene="ihfA"
                     /locus_tag="VD737_07510"
     CDS             complement(1371..1670)
                     /gene="ihfA"
                     /locus_tag="VD737_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HXW10443.1"
                     /translation="MALTKAEIAEALFDQLGLNKREARELVDLFFEELRMALANGEQV
                     KLSGFGNFDLRSKNQRPGRNPKTGEEIPISARRVVTFRPGQKLKARVEAYAGTQQ"
     gene            complement(1677..>3489)
                     /gene="pheT"
                     /locus_tag="VD737_07515"
     CDS             complement(1677..>3489)
                     /gene="pheT"
                     /locus_tag="VD737_07515"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HXW10444.1"
                     /translation="LERHAPGSDERFPVDLTSGAGCVRFASRVIRGLDPKAGSPAWMQ
                     ERLRRAGLRPISAAVDVTNYVMLELGQPMHAYDLRELAGGIVVRRARAGETLRLLDGR
                     EVLMDESVLVIADRQKPLGLAGVMGGDHSGIGDDTTDVMLEVAWFQPDVIAGRGRRYG
                     LVTDASQRFERGVDPTLQERAIERATALLCACAGGVAGPTQVTELADELPARSVVPLR
                     PDRARLVIGAPIADGTMASTLTALGMDLTRGESKWLVTAPSWRFDLAIEEDLIEEIAR
                     VHGFDNVPETVQPSRQPIAPATETRVRIETAADVLVQRGYFEAITYSFIEPKLQGMFA
                     PAMPALTLANPISAELATMRASLWPGLVSALAFNQRRQQSRVRLFESGRKFVIDGQGT
                     LEEIPVLAGLAAGPALPEQWGSARMPVDFFDVKADVEAVLHATGCASEFRFVAAAHPS
                     LHPGQAARILRGGAHVGWIGRLHPEVERELDLTYSALVFELDVESALRAAIPHFAEVS
                     RFPAVRRDLAVVVDEGVSVQSLLDCVHAAAGDVLRESVVFDIYRGAGIETGRKSVAIG
                     LNLQDVSRTLTDDATDAIVARVVADLERECSATIRDR"
BASE COUNT          586 a         1171 c         1120 g          612 t
ORIGIN      
        1 gagcgccgag attcgaactc gggaccccct tgcatcccat gccgattttc gtgcttttcc
       61 gtgctgatcc gttcgcatcg taaaacaccg cggaatgcct caactgtttg cgggggtctc
      121 tgatttacgc tgattggcta tagcgacaag ccatgcagcg tgacatttga cgtgaaagat
      181 caaggctaag gaatcggtct gttcgacctc aatttccatt tcgtcacttg tcgtaatccc
      241 ttggcatagc gcgcacggtg cgccagtgcc aggcccgcca aatgtccgaa atcgctccac
      301 ctgcggcaga cttccatcgg caacccgctg ctgcgccagt tgcagaagtt ccggattcac
      361 gtgccacccc tttgtttcgt tgccggttat tcgtacgggg tcgcgagcgg attcgatatt
      421 gctggaaatc gagcagaaat gtatgcggcg acttacggcc gaactgctcg atcggtctgc
      481 tgggcgaatt cggcccggag cacgcggcgc ctacttggag cgccgctgca cggggtccgc
      541 tacgctcgcc gagctcggcc tagtgaaaga gcctcagccg ctgccgcatt ggcgaacagg
      601 ccgatcgcgt cgaccgctcg cgcgttggga ttgactcgag cgacccactc gagcgtccgc
      661 cgcatgatct gcgcgtgggc acgccgcagc tcggcgtcca tccgctcgtg aatgcgggcc
      721 tcggcgctgc tcacagctcc ggtgctcatc gtctcaaccc tccttctgga actgaccaaa
      781 accgagcgta aggccgaagg gggttaagga tgaataacca agccgtgtcc gtgccgaagt
      841 gcccctaatg aaaaaaccgc ctttcggcgg tttctcgtaa gtgcttatct ttattggtcg
      901 gagcgccgag attcgaactc gggacccctt gcaccccatg caagtgcgct accagactgc
      961 gccacgctcc gaccgggcgc ggagtatagc gtactcggtc ggtggcttca gccgtgcgga
     1021 aaggaaccgc cggtcagcgc cgcaggatct tgatcagctc ttcgagttcg acgcgcagct
     1081 gtcgcgcgag ctggtgtgtc tgggtgacgt cgacgcgcgc ctcctcgccg tcgaggcgat
     1141 tgcgcgctcc gccgatcgtg aagccctggt cgtagagcaa cgcgcggatc tgccggatca
     1201 cgagcacgtc ctggcgctgg tagtaacgac ggttgccgcg acgcttgacg ggcttgagct
     1261 gcgggaactc ctgctcccag taccgcagca cgtgcggctt gacgccgcag agttcgctga
     1321 cctcaccgat cgtgaagtag cgcttgccgg ggatggtcgg cagttcgtcg ttattgctgg
     1381 gttccagcat acgcttccac gcgggccttc aacttctggc cgggacggaa cgtcacgacg
     1441 cgtcgcgcgc tgatcgggat ttcctcgccg gtcttcgggt tgcggcccgg ccgctggttc
     1501 ttgctgcgca ggtcgaaatt gccgaagccc gagagcttga cctgctcgcc gttcgcgagc
     1561 gccatgcgca gctcttcaaa gaacaggtcg acgagctcgc gcgcctcacg cttgttgagg
     1621 ccgagctggt cgaacagggc ttcagcgatt tccgccttgg tcaatgccat ttcgtcttat
     1681 cggtctcgta tcgtggcgga gcactcccgc tccaggtcgg cgacgacccg agccacgatg
     1741 gcatccgttg catcgtcggt aagggtgcgg gaaacgtcct gtaaattcaa gcctatcgcg
     1801 acactcttgc gacctgtttc gatgcctgcg ccgcgatata tgtcgaacac gacggactcg
     1861 cgcagcacat ccccggcggc ggcgtggacg cagtccagca gcgactgcac ggaaacgccc
     1921 tcgtccacga cgaccgcgag gtcgcggcgc acggcaggaa atcgcgaaac ctcggcgaaa
     1981 tgcgggatgg cggcacgaag tgcgctttcc acgtcgagct cgaacacgag tgcggaatat
     2041 gtcaagtcga gctcgcgttc cacttcggga tgcagccggc cgatccagcc gacgtgcgca
     2101 ccgccgcgca ggatcctggc ggcctgcccc gggtgcaacg acggatgtgc cgccgcgacg
     2161 aagcggaact cggacgcgca gccggtcgcg tgcagcaccg cctcgacgtc cgccttcacg
     2221 tcgaagaaat ccacgggcat gcgcgcgctg ccccactgct ccggcagcgc ggggcccgcg
     2281 gcgaggcccg ccaggaccgg aatctcctcg agcgtgccct gcccgtcgat cacgaacttg
     2341 cggcccgact cgaacaatcg cacgcgcgac tgctggcggc gctggttgaa ggccagcgcg
     2401 ctcacgagcc cgggccagag cgacgcgcgc atcgtcgcga gctcggcgga aatcggattg
     2461 gcgagcgtga gcgccggcat cgccggtgca aacattccct gcagcttcgg ctcgatgaag
     2521 ctgtaggtga tcgcctcgaa gtagccgcgc tgcacgagca cgtcggcggc agtctcgatg
     2581 cgcacgcgtg tttcggtcgc cggtgcgatc ggctgacgcg acggctgcac ggtctccggc
     2641 acgttgtcga agccgtgcac gcgcgcaatc tcctcgatca ggtcctcctc gatcgcgagg
     2701 tcgaaccgcc acgacggggc cgtgacgagc cacttggact caccgcgcgt aaggtccatg
     2761 ccgagcgcgg tcaacgtgga cgccatggtc ccgtcggcga tcggcgcgcc gatcacgaga
     2821 cgggcacggt cgggtcgcag tggcacgact gaccgcgcag gaagctcgtc ggcgagttcc
     2881 gtcacctgcg tcgggcccgc gacgcctccg gcgcacgcac acagcagggc cgtggcgcgc
     2941 tcgatggcac gctcctgcag cgtcggatcg acgccgcgct cgaagcgctg cgaggcatcc
     3001 gtgacgagtc catagcgccg gccgcgcccg gcgatcacgt ccggctgaaa ccaggcgact
     3061 tccagcatca cgtcggtcgt gtcgtcgccg atgccggagt ggtctccgcc catcacaccg
     3121 gcgaggccga gcggcttctg acggtccgcg atcaccagca cgctctcgtc catcaggact
     3181 tcgcggccgt cgagcagcct gagcgtctcg cccgcgcgtg cccgacgcac gacgatgccg
     3241 cccgcgagct cacggaggtc gtaggcgtgc atcggctggc cgagctcgag catcacgtag
     3301 ttggtgacgt cgaccgcggc gctgatcggc cgcaatcccg cccggcggag gcgctcctgc
     3361 atccaggccg gcgacccggc cttcgggtcc aggccgcgga tcacgcgcga cgcgaagcgc
     3421 acgcaaccgg cgcccgacgt caggtccacc gggaaacgct cgtccgaccc aggcgcgtga
     3481 cgttccagc
//
LOCUS       DASPFR010000282         2834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1055310, whole genome shotgun sequence.
ACCESSION   DASPFR010000282 DASPFR010000000
VERSION     DASPFR010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055310"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..853
                     /gene="lptB"
                     /locus_tag="VD737_07520"
     CDS             125..853
                     /gene="lptB"
                     /locus_tag="VD737_07520"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005096670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HXW10445.1"
                     /translation="MSRLRAEALGKSYRSRQVVKNLSLEVGSGEVVGLLGPNGAGKTT
                     AFYMISGLVPCDEGRIHLDDQELTRLPMYRRAQLGVGYLPQEASVFRKLSVEDNILAI
                     LETRDDLDEDEARNAVLEALLEELHIGHIRGSSGQSLSGGERRRVEIARALAAEPRFI
                     LLDEPFAGVDPISVIDIQRIIRHLSARNIGVLITDHNVRETLGICGRAYIVSAGSVIA
                     SGSAEEILANPQVREVYLGQDFRL"
     gene            885..2354
                     /locus_tag="VD737_07525"
     CDS             885..2354
                     /locus_tag="VD737_07525"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HXW10446.1"
                     /translation="MLKPALLLKLGQQLTMTPQLQQAIKLLQLPVLELQAQVQEALET
                     NVMLEQEEDETIDLASLEQSAPAGESETDGAETEVGGESDDGAAGEEVVVEMEDPWAE
                     SSTPSGDGARSDDDDRPLEFTDERGNDLHQHLIWQLEISPLDPRQLWIGEAIVDSLND
                     DGYLVESCAEIAHSLSADLPVAEEDVEAVLAFVQTLDPSGVGARTLSECLTLQLAQLD
                     AGTPGRALALSIARDHLQAVADRDLGALRRALGVDEDALQGALALVRACHPRPGSAFE
                     GAQPEYVVPDVFVRRTDQGWSVELNAGSVPRLKVNQGYAGLVARSADYASLRAQLQEA
                     RWLIRSLEIRNETLLKVARSIVQRQSAFLERGDEAMQPMILRDVAEAVGMHESTISRV
                     TTGKYMHTPRGIFEFRYFFSSHVSGTDGEDVSSVAIRARIRKLIGDEPPDKPLSDAQL
                     ATILAGEGVKVARRTVAKYREALGLASSSERRQVAARAQ"
     gene            2460..2786
                     /gene="raiA"
                     /locus_tag="VD737_07530"
     CDS             2460..2786
                     /gene="raiA"
                     /locus_tag="VD737_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HXW10447.1"
                     /translation="MQVNLSGHHVDITPALRTYVEKKLARVLRHSDRVIDVQCTLTVE
                     KLRHQAESTVWLAGTSIHAAAVHEDMYAAIDLLTDKLDEQVRRHKEKVRDPQAARHRH
                     GGELSP"
BASE COUNT          502 a          977 c          963 g          392 t
ORIGIN      
        1 tcgtctacga catcggtcgc gagcgcgtgc aggccaaccc gaacgagaag gacccgggcg
       61 gcgtccgcat cacgatcaac ccgccgaaga agccgggcag cggccagccg cagggcccgg
      121 gctcatgagc cggctgcggg ccgaggcact cggcaagagc tatcgctcgc gacaggtcgt
      181 caagaacctt tcgctcgaag tcggttcggg cgaggtcgtg gggctgctcg gccccaacgg
      241 agcgggcaag acgacggcct tctacatgat ttcaggactc gtgccctgcg acgagggccg
      301 gatccacctc gacgaccagg agctcacgcg cctgccgatg tatcgccggg cgcaactcgg
      361 cgtcggctac ctgccgcagg aagcctcggt gttccgcaag ctgtcggtcg aagacaacat
      421 cctcgcgatc ctcgagaccc gcgacgacct cgacgaagac gaggcgcgca acgccgtgct
      481 ggaggcgctg ctcgaggagt tgcacatcgg acacatccgc ggctcgagtg gccagagtct
      541 ttcgggcggc gagcggcggc gggtcgagat cgcacgggcg ctcgcggccg agccacgctt
      601 catcctgctc gacgagccgt tcgccggcgt cgacccgatc tccgtgatcg acatccagcg
      661 catcatccgg cacctgtccg cacgcaacat cggcgtgctg atcaccgacc acaacgtgcg
      721 cgaaaccctg ggcatctgtg gtcgtgccta catcgtcagc gccggaagtg tgatcgcgtc
      781 gggatcggcc gaagagatcc ttgccaatcc tcaggtgcgt gaggtctact tgggtcagga
      841 tttccgcctc taggcccgcc gggccgcgga cgaccgcctc ttccatgctc aagccggccc
      901 tgctgctcaa gctcggtcag cagctgacga tgacgccgca gttgcagcag gccatcaagc
      961 tgctgcagtt gcccgtgctt gagttgcagg cacaggtgca ggaggcgctc gagaccaacg
     1021 tgatgctcga gcaggaggag gacgagacga tcgacctcgc ctcgctcgaa cagtctgcgc
     1081 cggcgggcga gtccgagacc gacggcgccg agaccgaagt tggcggcgag agtgacgacg
     1141 gcgcggctgg cgaggaagtg gtcgtcgaaa tggaggaccc gtgggcggag tcctccacgc
     1201 cgtcgggcga cggcgcgcgg tccgacgacg acgaccgccc gctcgaattc accgacgagc
     1261 gcggcaacga cctgcaccag cacctgatct ggcagctgga gatcagcccg ctcgaccccc
     1321 ggcagctctg gatcggcgag gcgatcgtcg actcgctcaa cgacgacggt tacctggtcg
     1381 aatcctgcgc ggagatcgcg cacagcctca gcgctgacct gccggtcgcc gaggaagacg
     1441 tcgaggccgt gctcgcgttc gtccagacgc tcgatccctc cggcgtcggc gcccgcacgc
     1501 tcagcgaatg cctgacgctg cagctcgcgc agctcgatgc cggcacgccc gggcgggcgc
     1561 tcgcgctgtc gatcgcccgc gatcacctgc aggccgtcgc cgaccgcgac ctcggcgcgt
     1621 tgcgacgcgc gctcggcgtc gacgaagacg cgctgcaggg ggcgctcgcc ctggtgcggg
     1681 cctgccaccc aaggcccggc tcggcgttcg agggggcgca gcccgagtac gtcgtcccgg
     1741 acgtgttcgt gcgccgaacc gaccagggct ggagcgtcga gctcaacgcc ggctccgtcc
     1801 cgcggctcaa ggtcaaccag ggctatgcgg ggctcgtggc ccggtcggcc gactatgcct
     1861 cgctccgcgc ccagctgcag gaagcacgct ggctgatccg cagcctcgag atccgcaacg
     1921 agacgctgct caaggtcgcg cgatcgatcg tccagcgcca gtcggccttc ctcgagcgcg
     1981 gcgacgaggc catgcagccg atgatcctcc gcgacgtcgc cgaggccgtc ggcatgcacg
     2041 aatcgacgat ctcgcgggtg acgacgggca agtacatgca cacgccgcgc ggcatcttcg
     2101 agttccggta cttcttttcg agccacgtgt cgggcaccga cggcgaggac gtctcgtcgg
     2161 tggcgatccg ggcccgcatc cgcaagctga tcggcgacga accccccgac aagcccctca
     2221 gcgacgcgca gctggcgacg atcctggcgg gcgaaggggt caaggtggcg cggcgcaccg
     2281 ttgcgaagta ccgtgaggcg ctcggcctgg cttcgtccag cgagcgccgg caggtggccg
     2341 cacgggccca gtgacgactc cgccgcgggc ggggaacccg gccgtcaccc ggccatcgaa
     2401 caggatagag agagcgaaac agagagacga aaggcaggtt ccaacaggag gacgagacga
     2461 tgcaagtcaa cctcagcggt catcacgtcg atatcacccc cgcgctgcgt acgtacgtgg
     2521 aaaagaagct cgcgagggtc ctgcgccaca gcgatcgcgt catcgacgtc caatgcaccc
     2581 tgacggtgga aaaactgcga caccaggccg aatcgaccgt gtggctggcc ggcacgagca
     2641 tccacgccgc ggccgtccac gaggacatgt acgccgcgat cgacctgctg acggacaagc
     2701 tcgacgagca ggtccggcga cacaaggaga aagtccgcga cccgcaggca gcccgtcatc
     2761 ggcacggggg agaactgtcg ccttgagccc acgtccgggc gcgctttgat ccggaggtcg
     2821 ccggcgccca ccgg
//
LOCUS       DASPFR010000283         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1177910, whole genome shotgun sequence.
ACCESSION   DASPFR010000283 DASPFR010000000
VERSION     DASPFR010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177910"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..1304)
                     /locus_tag="VD737_07535"
     CDS             complement(36..1304)
                     /locus_tag="VD737_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXW10448.1"
                     /translation="MSTRSVRPSPGLANVRYEIRGQLARRALEMERMGYDIVSLNIGN
                     PGAFGFRTPETMRLAMIENLRGAEGYCHQKGIFPAREAVVMQQQERGVRGVTADEVFI
                     GNGVSELIDLTLRALLAADEEVLVPAPDYPLWTAAVNLNRGRAVHYPCRPTQGFVPDP
                     DELESLITKRTRAIVVINPNNPTGAVYPRAVLEAIARIAEQHHLVVFADEIYDQMTYD
                     GAEFVPMATLVHHTLCCTMSGLSKVYRACGYRVGWAAFSGDLENSREYLAGLELLSSL
                     RLCSNVAGQWAVQTALGGYQSIRELVSPGGRLYESRRAIVDAVARSRYLTLTPPMGAL
                     YAFVGVDPKTLPSFDDRRFAMDLLEHKHVLVAPGTSFNVPYNTHFRVTNLPEPAVLTD
                     VFGRIEELLDNYAAGDEPVSRPSPLRAVEA"
     gene            complement(1427..>2132)
                     /locus_tag="VD737_07540"
     CDS             complement(1427..>2132)
                     /locus_tag="VD737_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXW10449.1"
                     /translation="LVLTAISIGFVHTLIGPDHYLPFVAMSAARNWTRRKTLLVTAAC
                     GVGHVLGSIVLGLVGIAIGAALHRLEWIEGVRGDLAAWALTAFGLVYMAWGLRRAWRD
                     REHSHEHVHSDGTRHRHTHAHHTRDEHLHVHVQVDTDNVRSLTPWALFIVFILGPCEA
                     LIPLLMYPASQHSWWGVSLVVIAFAVTTILTMLTIVYLAVRGLERLPLKAAERYTHAL
                     AGAALSVCGLGIVFGL"
BASE COUNT          351 a          705 c          747 g          329 t
ORIGIN      
        1 cgaagcttgc gtgcaattcc ccgaaaacac tcgaactacg cttcgacggc ccgcaacgga
       61 ctgggacgcg acaccggctc gtcgccggct gcatagttgt ccagcagttc ctcgatccgc
      121 ccgaacacgt ccgtcaacac ggccggctcc ggcaggttcg tgacacggaa gtgcgtgttg
      181 tagggcacgt tgaagctggt gcccggcgcg accagcacgt gcttgtgctc gagcagatcc
      241 atggcgaacc gtcgatcgtc gaagctgggc agcgtcttcg gatcgacgcc gacgaacgcg
      301 tagagcgcgc ccatcggcgg cgtcagcgtg aggtacctgc tgcgcgcgac cgcgtcgacg
      361 atcgcgcggc gcgattcgta caggcgtccg ccgggactca cgagctcgcg gatgctctgg
      421 taaccgccga gcgcggtctg caccgcccac tggccggcga cgttgctgca gagacgcagc
      481 gacgacagca gttcgaggcc tgcaagatat tcgcgggagt tctcgaggtc gcccgagaac
      541 gcggcccagc cgacccggta tccgcaggcg cggtagacct tcgacaggcc gctcatcgtg
      601 cagcagagcg tatggtgcac gagcgtcgcc atcggcacga actccgcgcc gtcgtacgtc
      661 atctggtcgt agatctcgtc cgcgaagacg accagatggt gctgctcggc gatccgggcg
      721 atcgcctcga ggaccgcgcg cgggtacacg gcgccggtcg gattgttcgg gttgatcacc
      781 acgatcgcgc gcgtgcgctt ggtgatcagc gactcgagtt cgtcggggtc cggcacgaac
      841 ccctgcgtgg gacggcacgg gtagtgcacc gcgcggccgc ggttcaggtt cacggccgcg
      901 gtccagagcg ggtagtccgg ggccggcacc agtacctctt cgtcggcggc cagcaacgcg
      961 cgcagcgtca ggtcgatcag ttcgctgaca ccgttgccga tgaacacctc gtccgccgtc
     1021 acgccgcgga cgccgcgctc ctgctgctgc atgacgacgg cttcacgcgc cgggaagatg
     1081 cctttctggt ggcagtagcc ttcggccccg cgcaggttct cgatcatcgc gaggcgcatc
     1141 gtctccggcg tgcggaagcc gaacgcgccc gggttgccga tgttcaggga gacgatgtca
     1201 tagcccatgc gctccatttc gagggcacga cgggcaagct ggccacggat ctcgtaacgg
     1261 acgttggcga ggccggggct cgggcggacg ctgcgggtgg acatgggtgg gccggtgcaa
     1321 ccgtaacccc tggattataa gggcatggcc gacgggggtt gcgggagact cggaaagtgc
     1381 gctgcgcgca cgggttgccg tgcgctccgg cgatgccgtt ccggtcctag agtccgaaaa
     1441 cgattcccag tccgcacacg ctcagcgccg cgccggcgag cgcgtgcgtg tagcgctcgg
     1501 ccgccttgag cggcaggcgt tcgaggccgc gaaccgcgag gtacacgatc gtcagcatcg
     1561 tcaggatcgt cgtcaccgcg aacgcgatca ccaccagcga cacgccccac cagctgtgct
     1621 gcgaggcggg atacatcagc agcgggatca gcgcttcgca cggcccgagg atgaacacga
     1681 tgaacagcgc ccacggcgtc aggctgcgga cgttgtcggt gtcgacctgg acgtgcacgt
     1741 gcaggtgctc gtcgcgcgtg tggtgcgcgt gcgtgtggcg gtgacgcgtg ccgtcggaat
     1801 gcacgtgctc gtgcgaatgc tcgcggtcgc gccaggcgcg cctcaaaccc cacgccatgt
     1861 acaccagccc gaaggcggtc agcgcccagg cggcgaggtc gccgcgcacg ccttcgatcc
     1921 attccagccg atgcagtgcc gcgccgatcg cgatgccgac gaggcccagc acgatcgagc
     1981 cgaggacgtg gcccacgccg caggcggcgg tcacgagcag cgtcttgcgc cgggtccagt
     2041 tgcgggccgc ggacatggcg acgaacggca ggtagtggtc cggcccgatc agcgtgtgca
     2101 cgaagccgat cgagatggcg gtcagcacca gc
//
LOCUS       DASPFR010000284        13515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_689197, whole genome shotgun sequence.
ACCESSION   DASPFR010000284 DASPFR010000000
VERSION     DASPFR010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13515
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689197"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1841)
                     /locus_tag="VD737_07545"
     CDS             complement(<1..1841)
                     /locus_tag="VD737_07545"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HXW10450.1"
                     /translation="MSTAVKFHSKDARSIPFQEASLDIWDKKYRLSAKDGTPIDQTMD
                     DTYKRVARALADVEAPELREKWYERFLWALRRGAIPAGRVTSNAGAQEHKPATSTINC
                     TVSGTIRDSMDDILGKVHEAGLTLKAGCGIGYEFSTLRPRGAYVSGAGAYTSGPLSFM
                     DIYDKMCFTVSSAGGRRGAQMGTFDIGHPDVMEFIRAKREAGRLRQFNLSLLVTDGFI
                     EAVREDREWQLAFPVREAEVAGEDVTDPHKYVWREWPDPASYVRNDEGLVLCKVYKTL
                     PARRMWDVIMTSTYDFAEPGFVLIDRVNEMNNNWWCENIRATNPCGEQPLPPYGSCLL
                     GSINLTSFVRDPFTDEARFDWDEYRAVVKVFTRMLDNVVEVNGLPLEQQRNEIHRKRR
                     HGMGFLGLGSTITMLRMKYGSREAVGFTERVSKEMALAGWEAGLDLAEEKGAAPIMDE
                     EFVVTREMLRKRPEMARDGIKAGDRLPGRVLHAKYSRYMQRVAEVAPELVDKIARQGA
                     RFTHHSSIAPTGTISLSLANNASNGIEPSFAHHYFRNVIREGRKTKEKVDVFSFELLT
                     YREIINHDAMPNATDAAKKLPEYFSTADDVTPTQHVDIQAAAQLWVDS"
     gene            2453..3811
                     /locus_tag="VD737_07550"
     CDS             2453..3811
                     /locus_tag="VD737_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HXW10451.1"
                     /translation="MKLQPSFAAFLLACTFTFWTAPASAQLPAAVGDTPVPSLAPMLK
                     RVSPAVVNIATRGTVQEQNPLLNDPFFRRFFDVPNTPRQREFQSAGSGVIVDSKNGYI
                     ITNAHVVENATDITVQLLDNRSLKAKVVGTDPGSDVAVLQVQAGNLVDLPIADSDRAE
                     VGDFVVAIGNPFGLGHTVTSGIVSALGRSGINPEGYEDFIQTDASINPGNSGGALVNL
                     SGQLVGINSAILSRSGGNIGIGFAIPSNMMKVVMNQLIKFGKVKRGVLGVNIQTLVPE
                     IAQSMGLQEGIQGALVSQVVEGSAAEKAGVRAGDVITGINNRAVRDAGQLRNAIGLLS
                     VGEKVDIALLRDGKPRRVTATISERDAAAEARAGGTATGSHPGLEGADIADGPNGVVI
                     RSVAEGSPAAQRGLRTNDVILAVGRTRVANVAEFQRATEGQSAFVLQIRRGNAMLVIP
                     IR"
     gene            3897..4826
                     /locus_tag="VD737_07555"
     CDS             3897..4826
                     /locus_tag="VD737_07555"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HXW10452.1"
                     /translation="MPKTLEVCVLGGTGFIGGEVVARIAALGHDVKVLTRNPGQSRDL
                     KVLPSVRFVRADVHDEARLEREISGCDVVVNLVGILNERGLGRGSGAEFRRVHTELTQ
                     KVVRACRAAGVRRYLHMSGLKADSRRGPSHYLRSKGLAEDYIREHCADGEPEYVIFKP
                     SVVFGPGDAFINNFAGILKLTPGVLPLACANAKFAPVYVGDVAEAFVACLERRDVAGK
                     TFELCGPDVMTLGDIVRMTAQVLGLRRVVVPLPDAVSRVQAAVMDFVPGKPFSTDNYL
                     SATLDSTCDCDGLGELGIERTPLRGTVATYLNK"
     gene            complement(5025..6068)
                     /locus_tag="VD737_07560"
     CDS             complement(5025..6068)
                     /locus_tag="VD737_07560"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HXW10453.1"
                     /translation="MSLARVLALLVLCTTLSGCYVMQAANGQLEVLRRSRPIEQVLAD
                     PTTPAATRRGLELTVAARSFAETELGLPDSRSYRKYADLGRPFVVWNVVATPEFSVEP
                     RRWCFPVAGCVAYRGYFRESAARSHALKLAMRGDDVSVGGVPTYSTLGHLPDPVFNAM
                     LGWRDTRLVGTIFHELAHERLYVAGDSEFNEAFASVVEEAGLRRWLQALGRDDELERF
                     EVGARREAEFARLLRETRRRLTQLYESDLPDADKRVEKQREFGRLQFDYERLRAAWGG
                     YPGYDGFFQRPLNNAHLASVATYHDCVPGLRLELERAGSMQEFYERAGELAKLSSRGR
                     RRAVCRAEGSSAE"
     gene            6120..7352
                     /locus_tag="VD737_07565"
     CDS             6120..7352
                     /locus_tag="VD737_07565"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008137.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HXW10454.1"
                     /translation="MDVYLVGGAVRDALLGREATEHDYVVVGATPDDLLARGFRPVGK
                     DFPVFLHPDNGDQYALARTERKTGAGYYGFATRFSPDVTLEDDLARRDLTINAMARAE
                     DGTVVDPYGGRRDLAARVLRHVSPAFVEDPLRVLRVARFAARFAPLGFTVAPETLALM
                     RAIVEAGEMQALVAERVWVETDRALGEARPAVYIEVLRECGALAAVFPEIDRLFGVPQ
                     PEKYHPEIDTGVHTLQVLDVACELSQDTVVRFAALVHDLGKGVTPPHEWPRHIGHEHA
                     GVKEIARLCDRLKVPAEHRELGTLTSKEHQRVHRARELRDATVLELLEATDAFRRPER
                     FEKLLLACEADARGRGPELRQRPYDQGRLLREWRRAAAAAKLDPQVLASESGPSIARL
                     LRTARVAAIRAARQNEAL"
     gene            complement(7372..8169)
                     /locus_tag="VD737_07570"
     CDS             complement(7372..8169)
                     /locus_tag="VD737_07570"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001393.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HXW10455.1"
                     /translation="MDLVQAIVLAVVQGLSEFLPISSSGHLVLIPHFFGWPDQGLAFD
                     VAVHLGTLAALLVYFRRQLWTMATAWLGSVTRQQHTRDSRLAWQLLVATIPVGLVGLL
                     FNDFIEENLRNPLFVAGTLTFFGLLMYAADRWGRGDRDEFSLSWGQSFLIGCAQALAL
                     MPGTSRSGITMTAGRALGLSRSGAARFSFLLAIPGIGAAGAYEGLKLLTSDHPVDWLP
                     MFVGLVFAALSGLACIHLLIRFIERIGLLPFTLYRLLIAAVIVWHFT"
     gene            complement(8215..9405)
                     /locus_tag="VD737_07575"
     CDS             complement(8215..9405)
                     /locus_tag="VD737_07575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXW10456.1"
                     /translation="MTVATGLPELSAEEAAHGERLLARIAEAIAGAGGWMSFDDFMRM
                     ALYEPGLGYYSAGARKFGAAGDFVTAPEVAPVFSRCLAVQCAEVLRELGPRAEVLELG
                     AGSGAMACDLLLELERRDALPGRYLILDVSADLRERQREALAAAAPHLLERVRWLDRL
                     PAEPFDGLVVANEVLDALVVERFVKRGDRVMALGVVWDGPGGHLRLAERPARPKLEDA
                     VRRLEADLGRALPDDYVSEINLGLDDWLRSIASCVRRGVLLFVDYGLPRHEYYAAERS
                     RGTLLCHFRHRFHDDALARVGLQDITAWVDFTAVAEGAQAAGLDVAGYTTQAHFLIGC
                     GLEEFIAHVGDLDVVQRVNLSRQAMVLTLPGEMGERFKVIALAKGYDAPLRGFGVRDL
                     RHTL"
     gene            9539..10255
                     /locus_tag="VD737_07580"
     CDS             9539..10255
                     /locus_tag="VD737_07580"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF006598.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HXW10457.1"
                     /translation="MALITGGARRLGAAIARRLHDAGARVAVHYRSSATDATALVAEF
                     HAVRADSAVAVAGDLLDTDAIPSIVEEATRRFGRLDVLVNNASSFYATPLGSITPGQF
                     DDLIGTNLRAPLLLAQAAAPALRQARGLILNMADIHGQRPLRDHAVYSAAKAGLIMLT
                     RSLARELGPEVRVNAIAPGPVLWPESGMAADLQAEIVGKTALLRSGSPEDIAAAAYFF
                     AAEAPYVTGQVLAIDGGRSL"
     gene            complement(10263..10766)
                     /gene="folK"
                     /locus_tag="VD737_07585"
     CDS             complement(10263..10766)
                     /gene="folK"
                     /locus_tag="VD737_07585"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HXW10458.1"
                     /translation="MTRDVLVAAGSNVAPIANLRRALEALRRHFPGLQVSPAYANAAV
                     GFEGDDFVNLVVRFDTDLPVQQVLARLHEAEAACGRERDAPKWAPRAMDLDVLLYGDL
                     VCAERGFTLPRPDLLRRPYMLGPAAEIAPEVEHPTARRTLAELWREMQLRAPAHDLRR
                     IELAESR"
     gene            complement(10780..11169)
                     /gene="folB"
                     /locus_tag="VD737_07590"
     CDS             complement(10780..11169)
                     /gene="folB"
                     /locus_tag="VD737_07590"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HXW10459.1"
                     /translation="MTDTIFLGGLTAECIIGIWDWERKVRQKVVIDLEMAADIRRAAA
                     SDHIDDTLDYKRVSKRLQQFIEQSQFQLVETLTDRIAQLVITEFGVPAVKVRLNKQGA
                     LRGARDVGIVIERRREDYLPPDSRAGT"
     gene            complement(11212..12246)
                     /gene="tsaD"
                     /locus_tag="VD737_07595"
     CDS             complement(11212..12246)
                     /gene="tsaD"
                     /locus_tag="VD737_07595"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HXW10460.1"
                     /translation="MRVLGIETSCDETAAAVYDGERGLLAHELYSQVELHARYGGVVP
                     ELASRDHLRKLLPLIEKTLAVAGGRSGIDGIAYTSGPGLVGALLVGAAVAQGLAYAWR
                     VPSVGVHHMEGHLLAPLLEPKPPEFPFVALLVSGGHTLLARVRRIGEYELLGSSLDDA
                     AGEAFDKTAKLLGLGYPGGPQLAALAERGRPDRFRFPRPLTERPGLDFSFSGLKTAVV
                     VALRNHVGELDAQARADVARGFEEAVVDTLVIKCRRALEATDSRTLVVAGGVGANRRL
                     RARMLEMGEREGVRVVYPRAEFCTDNAAMIALLGHLRLAAGERDDRSIRARARWPMTE
                     LVPPAPAARG"
     gene            12439..12660
                     /gene="rpsU"
                     /locus_tag="VD737_07600"
     CDS             12439..12660
                     /gene="rpsU"
                     /locus_tag="VD737_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HXW10461.1"
                     /translation="MPSVRIRENEYFDAALRRFKRACEKAGVLTELRRREFYEKPTQE
                     RKRKKAAAVKRHQKRSTRDFTSAPRARTY"
     gene            12821..13285
                     /locus_tag="VD737_07605"
     CDS             12821..13285
                     /locus_tag="VD737_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HXW10462.1"
                     /translation="MSSSTAPSLKERITEDMKSAMRGGDKERLGTIRMLQAAIKQREV
                     DERITLDDAQVLAVIEKMVKQRKESIVQFEQGGRADLAAKEKAEIEQLQAYLPAQLSD
                     AAVDALIREAIAATGASSIKDMGKVMGAVKAKAAGRADMGAVSARIKAALGA"
BASE COUNT         2173 a         4766 c         4580 g         1996 t
ORIGIN      
        1 gaatcgaccc agagctgcgc ggcggcctgg atgtcgacgt gctgcgtggg cgtcacgtcg
       61 tcggccgtcg agaagtactc gggcagcttc ttcgccgcat cggtggcgtt cggcatggcg
      121 tcgtggttga tgatctcgcg ataggtcagg agctcgaacg agaacacgtc gaccttttcc
      181 ttcgtcttgc gcccctcgcg gatcacgttg cggaagtagt gatgcgcaaa cgacggctcg
      241 atgccgttcg aggcgttgtt cgccagcgac aacgagatcg tgccggtggg tgcgatcgag
      301 ctgtggtggg tgaagcgagc gccctggcgc gcgatcttat cgacgagctc cggggcgacc
      361 tcggcgacgc gctgcatgta gcggctgtac ttcgcgtgca gcacgcggcc cggcagcctg
      421 tcgccggcct tgatgccgtc gcgggccatt tccggccgct tgcgcagcat ttcgcgcgtc
      481 acgacgaact cctcgtccat gatcggcgcc gcgcctttct cctcggcgag atcgaggccc
      541 gcttcccatc ccgcgagcgc catttccttg gacacgcgtt cggtgaagcc cacggcctcg
      601 cgcgagccgt acttcatgcg cagcatcgtg atggtcgagc cgagcccgag gaatcccatg
      661 ccgtggcggc gcttgcgatg gatctcgttg cgctgctgtt cgagcggcag cccgttgacc
      721 tcgaccacgt tgtcgagcat ccgggtgaac accttgacga ccgcgcggta ttcgtcccag
      781 tcgaagcgcg cctcgtcggt gaacgggtcg cgtacgaacg acgtgaggtt gatcgagccg
      841 agcaggcacg agccgtaggg cggcaatggc tgctcgccac aggggttggt ggcgcggatg
      901 ttctcgcacc accagttgtt gttcatctcg ttgacgcggt cgatcaatac gaagccgggc
      961 tcggcgaagt cgtaggtgga cgtcatgatc acgtcccaca tgcgccgcgc cggcagcgtc
     1021 ttgtagacct tgcagagcac cagcccttcg tcgttgcgga cgtagctggc cgggtccggc
     1081 cactcgcgcc agacgtactt gtgcggatcc gtgacgtcct cgcccgcgac ctcggcctcg
     1141 cgcacgggga aggccagctg ccactcccgg tcttcgcgca cggcctcgat gaacccgtcg
     1201 gtgaccagca gcgacaggtt gaactgccgc aggcgcccgg cctcgcgctt ggcacggatg
     1261 aactccatga cgtcgggatg gccgatgtcg aacgtgccca tctgcgcgcc gcggcgcccg
     1321 ccggccgacg acaccgtgaa gcacatcttg tcgtagatat ccatgaacga cagcgggccc
     1381 gaggtatagg cgcccgcgcc cgagacgtac gcgccccgcg ggcgcagcgt cgagaattcg
     1441 tagccgatgc cgcaaccggc cttcagcgtc agcccggctt cgtggacctt gcccaggatg
     1501 tcgtccatcg aatcgcggat cgttcccgac accgtgcagt tgatggtcga ggtggccggc
     1561 ttgtgctcct gcgcgcccgc gttcgacgtg acgcgcccgg ccgggatcgc gccgcggcgc
     1621 agcgcccaca ggaaccgctc gtaccacttc tcgcggagct ccggcgcctc cacgtcggcc
     1681 agcgcgcggg ccacgcgctt gtacgtgtcg tccatggtct ggtcgatggg cgtaccgtcc
     1741 ttggcggaca ggcggtactt cttgtcccag atgtcgagcg aggcttcctg gaaaggaatg
     1801 ctgcgagcat ctttcgagtg gaatttcacg gcagtgctca tttcggcgac acggctcccc
     1861 aggatcacga tggcggcaat ggatgggtgc aggcggaccc cctcggacga cgcagcgggt
     1921 ggcccccgtc ggcgtcggat ccgtcccgac ccccgcagga ccgcttggaa cgcgccttcg
     1981 aggccccctg gcggcgttcg tacggacaca acatctaggg tcaggggggc aagactatca
     2041 ccgcccccac ggtcgtcaac tgcttgtttc cggggtggcc cccgcaggcg gaaacatggt
     2101 caaaaattga gcagtttcaa tgcattgcag caaacgtgac cgatagatga cacctgccgg
     2161 aatcgactct ggcgtcaagt gattgtttct cacggaaaca caacatcttg tgttgcggtc
     2221 tatccacgcc ggacgcaccg ttgacgcacc gccggcgcac acgatatcca cacggttgcc
     2281 cacaagcggg ccgcaggacc ggctgccgct cagcgaagcg cgcggaactc gctcgccacg
     2341 gcgcagtccc atgcgtggac cgaccggcgc cgccggcgcc gcgcgattct tcgctgcgct
     2401 cgcccgcgcc gctccagccg cactagcacg tacccaggaa accagacgcc tcatgaagct
     2461 tcagccgagt tttgccgcct tcctgctcgc atgtactttt actttctgga cggcgcccgc
     2521 cagcgcacag ttgcccgccg ccgtcggcga tacgcctgtc ccctcgctcg cgccgatgct
     2581 caagcgcgtc agccccgcgg tcgtcaacat cgcgacgcga ggcacggtgc aggagcagaa
     2641 ccccctgctc aacgacccgt tcttccgccg cttcttcgac gtaccgaaca cgccacgcca
     2701 gcgcgagttc cagagcgccg gttcgggcgt catcgtggat tcgaagaacg gctacatcat
     2761 caccaacgcg cacgtcgtcg agaatgccac cgacatcacg gtgcaactgc tcgacaaccg
     2821 ttcgctcaag gccaaggtgg tcggcaccga ccccggctcc gacgtcgccg tgctgcaggt
     2881 gcaggccggc aatctcgtcg acctgccgat cgccgattcc gaccgcgccg aagtcggtga
     2941 cttcgtcgtc gcgatcggca atccgttcgg cctcggccac accgtcacct ccggcatcgt
     3001 gagcgcgctg gggcgctcgg gcatcaaccc ggaaggctac gaggacttca tccagaccga
     3061 cgcgtcgatc aaccccggca actcgggcgg cgcgctcgtg aacctgagcg gccagctggt
     3121 cggcatcaac tccgccatcc tgtcgcgctc cggcggcaac atcggcatcg gcttcgcgat
     3181 cccctcgaac atgatgaagg tggtgatgaa ccagctgatc aagttcggca aggtcaagcg
     3241 gggcgtgctc ggcgtgaaca tccagacgct ggtgcccgag atcgcgcaga gcatgggcct
     3301 gcaggaaggc atccagggcg cactggtgtc tcaggtcgtc gagggttcgg ccgcggagaa
     3361 ggccggcgtg cgcgcgggcg acgtcatcac gggcatcaac aatcgcgcgg tgcgcgacgc
     3421 ggggcagttg cgcaacgcca tcggcctgct gagcgtcggc gagaaggtcg acatcgcctt
     3481 gctgcgcgac ggcaagccgc gccgcgtgac cgccacgatc agcgagcgcg acgccgcggc
     3541 cgaggcccgc gcgggcggta ccgccacggg gtcgcacccc gggctcgagg gcgcggacat
     3601 cgccgacggc ccgaacggcg tcgtgatccg cagcgtcgcc gaaggcagcc cggcggcgca
     3661 gcgtggcctg cgcaccaacg acgtgatcct ggcggtcggc cgcacgcgcg tcgccaacgt
     3721 cgccgagttc cagcgcgcga ccgagggcca gtcggcgttc gtactgcaga tccgccgcgg
     3781 caatgcgatg ctggtgatcc cgatccgctg agcaaccatc gagcggaacg cgcccgaatc
     3841 cgcgggcgcg ttccgagcga accgccggtc gtgttcgtaa ccggcccgga ggcaaggtgc
     3901 cgaagactct cgaggtctgc gtgctcggcg gcaccggctt catcggcggc gaagtcgtgg
     3961 ctcgtatcgc cgcgctcggc cacgacgtca aggtgctcac ccgcaatccg ggccagagcc
     4021 gcgacctcaa ggtgctgccg tcggtgcgct tcgtacgcgc cgacgtgcac gacgaggcgc
     4081 ggctcgagcg cgagatctcc ggctgcgacg tggtcgtcaa cctggtcggc atcctgaacg
     4141 agcgcgggct cggtcgcggc agcggcgccg agttccgccg cgtgcacacc gagctgacgc
     4201 agaaggtcgt gcgcgcgtgc cgcgccgcgg gcgtgcggcg ctacctgcac atgagcggcc
     4261 tcaaggccga ctcgcggcgt ggacccagtc actacctgcg cagcaagggc ctcgccgagg
     4321 attacatccg cgagcactgc gcagacggcg agcccgagta tgtgatcttc aagccgtcgg
     4381 tggtgttcgg ccccggcgac gcgttcatca acaacttcgc cgggatcctg aagctcaccc
     4441 ccggggtgct gccgctcgcc tgcgccaatg cgaagttcgc gccggtgtac gtgggcgacg
     4501 tcgccgaagc gttcgtcgcg tgcctcgagc gacgcgacgt ggccggcaag accttcgagc
     4561 tctgcgggcc ggacgtcatg acgctcggcg acatcgtgcg catgaccgcg caggtgctcg
     4621 gcctcaggcg cgtggtcgtg ccgctgcccg acgccgtgtc acgcgtccag gcagccgtga
     4681 tggacttcgt gcccggcaag ccgttctcga ccgacaacta cctgtcggcc acgctcgact
     4741 ccacctgcga ctgcgacggc ctcggcgaac tcggcatcga gcgcacgccg ctgcgcggca
     4801 ccgtcgccac ctacctcaat aaatagggac actccgaatt tccggcgaat tggggacact
     4861 cctgatttat tgcgaagctg gtccgatcgc cgggatttgc aggctttccg cgcgtctcca
     4921 tgaccgtcct cctccgcgag aatggccgat tggacgccct accccgggaa acaccgaacg
     4981 aaaaacagga atgtccccaa ttagcgggaa attcggagtg tcccctattc cgcggatgag
     5041 ccctcggcgc ggcagacggc gcggcggcgc ccgcgcgacg acagcttcgc cagctccccg
     5101 gcacgctcgt agaactcttg catggacccc gcgcgctcga gctcgagccg cagccccggc
     5161 acgcagtcgt ggtacgtcgc gaccgacgcg aggtgtgcgt tgttgagcgg ccgctggaag
     5221 aaaccgtcgt acccgggata gccgccccag gccgcgcgca gccgctcgta atcgaactgc
     5281 agccgcccga actcgcgctg cttctcgacg cgcttgtcgg cgtccggcag gtccgattcg
     5341 tacagctgcg tcaggcgacg gcgcgtctcg cgcagcagcc gcgcaaactc ggcctcgcgc
     5401 ctcgccccga cctcgaaccg ctcgagctcg tcgtcgcgcc ccagggcctg cagccagcga
     5461 cgcagcccgg cctcctcgac cacgctcgca aacgcttcgt tgaactcgct gtcaccggcg
     5521 acgtacagcc gttcgtgcgc gagctcgtgg aagatcgtcc cgaccagccg cgtgtcgcgc
     5581 caaccgagca tcgcgttgaa caccgggtcg ggcaggtgcc cgagcgtcga gtacgtcggc
     5641 acgccaccga cgctcacgtc gtcaccgcgc atggcgagct tcagcgcgtg cgaacgcgcg
     5701 gccgattcgc gaaagtagcc gcggtatgcg acgcagcccg cgaccgggaa gcaccagcgg
     5761 cgcggctcga ccgagaactc gggcgtcgcg acgacgttcc agaccacgaa cggacgcccg
     5821 aggtcggcgt acttgcggta gctgcggctg tccggcaagc cgagttcggt ctcggcaaac
     5881 gagcgcgccg cgacggtgag ttccagtccg cgacgcgtcg cggccggcgt cgtcggatcg
     5941 gcgagcacct gctcgatcgg gcggctgcgg cgcagcacct cgagctgccc gttggccgcc
     6001 tgcatcacgt agcaaccgct cagggtcgtg cacagcacca gcagggcgag gacgcgagcc
     6061 agggacatgc gccgaagagt aaccgcagtg cccgcggcgt ttccgctacg attcccgata
     6121 tggatgttta cctggtcggt ggcgcggtgc gcgacgcgct gctcggccgc gaagcgacgg
     6181 agcacgatta cgtcgtcgtc ggcgcgacgc cggacgacct gctcgcgcga ggcttccggc
     6241 cggtcggcaa ggatttcccg gtcttcctgc atcccgacaa tggcgaccag tacgcgctcg
     6301 cgcgcaccga gcgcaagacc ggcgcgggct actacggctt tgcgacgcgc ttctcgccgg
     6361 acgtgacgct cgaggacgac ctcgcacgtc gcgacctgac gatcaatgcg atggcccgtg
     6421 ccgaggacgg caccgtcgtc gatccctacg gcggacggcg cgacctcgcg gcgcgcgtgc
     6481 tgcgtcacgt gtccccggcg ttcgtcgagg atccgctgcg ggtgctgcgt gtcgcgcgct
     6541 tcgccgcgcg gttcgcgccg ctcggtttca ccgtcgcacc ggagacgctc gcgctgatgc
     6601 gtgcgatcgt cgaggccggc gagatgcagg cgctcgtcgc cgaacgcgtc tgggtcgaga
     6661 ccgatcgcgc actcggcgag gcgcggcctg ccgtttacat cgaggtgctg cgcgagtgcg
     6721 gcgcgctcgc ggccgtgttc ccggagatcg accggctgtt cggcgtcccg cagccggaga
     6781 agtaccaccc cgagatcgac accggagtgc atacgctgca ggtgctcgac gtcgcgtgcg
     6841 agctgtcgca ggacaccgtg gtgcggttcg cggcgctcgt gcacgacctc ggcaagggcg
     6901 tcacgccacc gcacgagtgg ccgcgccaca tcggccacga gcacgccggc gtcaaggaga
     6961 tcgcccgtct ctgcgatcgg ctcaaggtcc cggcggagca ccgcgagctc ggcacgctga
     7021 cctcgaagga gcaccagcgg gtgcaccggg cgcgcgagct gcgcgatgcc acggtgctcg
     7081 agctcctgga ggcgaccgac gcgtttcgcc ggcccgagcg gttcgagaag ctgctgctgg
     7141 cctgcgaggc cgatgcgcgc ggccgcgggc ccgaattgcg tcagcgaccg tacgaccagg
     7201 ggcggctgct gcgcgagtgg cgccgggcgg ccgccgcggc gaaactcgac ccgcaggtgc
     7261 tcgcaagcga gagcggcccg tcgatcgccc ggctgctgcg caccgcgcga gtcgcggcga
     7321 tccgcgcggc gcggcagaac gaggccctct gacggccggc ggttcagcgg ctcaggtgaa
     7381 gtgccagacg atcaccgcgg cgatcagcag ccggtacaac gtgaacggca gcaggccgat
     7441 ccgctcgatg aagcggatca gcaggtggat gcaggcgagg ccgctcagcg ccgcgaacac
     7501 gaggcccacg aacatcggca gccagtcgac cggatggtcg gacgtgagca gcttgagccc
     7561 ttcgtaggcg ccggccgcgc cgatgccggg gatcgcgagc aggaacgaga accgcgccgc
     7621 gcccgagcgc gacaggccga gcgcgcgtcc cgcggtcatc gtgatgcccg accgcgaagt
     7681 gcccggcatc agcgccaggg cctgcgcgca accgatcagg aacgactggc cccagctcag
     7741 gctgaactcg tcgcgatcgc cacggcccca ccggtccgcc gcgtacatca gcaagccgaa
     7801 gaatgtgagc gtgccggcga cgaacagcgg gttgcgcagg ttctcctcga tgaagtcgtt
     7861 gaacagcagc ccgaccaggc cgacgggaat cgtcgcgacg agcaactgcc acgcgagccg
     7921 gctgtcgcgc gtgtgctgct gccgcgtgac cgacccgagc cacgccgtcg ccatcgtcca
     7981 gagctgccgg cggaagtaca cgagcagcgc ggcgagcgtc cccaggtgca cggccacgtc
     8041 gaaggcgagc ccctggtcgg gccagccgaa gaagtgcggg atcaggacga ggtggccgga
     8101 actcgagatc ggcaggaatt cggagagacc ctgtacgacg gcgaggacga tcgcttgaac
     8161 gagatccatg gatacctgtg agttcgagtc ttgttctgct gcggccggcg agggtcagag
     8221 cgtgtggcgc aggtcgcgca cgccgaaccc gcgcagcggc gcatcgtacc ctttcgcgag
     8281 cgcgatcacc ttgaaccgtt cgcccatttc gccgggcagc gtcagcacca tcgcctgccg
     8341 ggaaaggttg acgcgctgga ccacgtcgag gtcgcccacg tgggcgatga attcctcgag
     8401 cccgcagccg atcaggaaat gcgcctgcgt cgtatagccc gcgacgtcga ggcccgccgc
     8461 ctgcgcgccc tccgccaccg cggtgaaatc gacccaggcg gtgatgtcct gcaagcccac
     8521 gcgcgcgagc gcgtcgtcgt ggaaacggtg ccggaagtgg cacagcagcg tgccgcgcga
     8581 ccgctcggcc gcgtagtact cgtgccgcgg cagaccgtag tcgacgaaga gcagcacgcc
     8641 ccggcgcacg caggaggcga tcgaccgcag ccagtcgtcg aggccgagat tgatttccga
     8701 cacgtagtcg tccggcagcg cgcgacccag gtccgcctcg agccgtcgca cggcgtcctc
     8761 cagcttcggc cgggccggac gttcggcgag ccgcaggtgc ccacctggac cgtcccagac
     8821 gaccccgagc gccatcaccc ggtcgccgcg ctttacgaac cgctcgacga cgagcgcatc
     8881 cagcacttcg ttcgcgacga cgagcccgtc gaacggctct gccggcagcc ggtcgagcca
     8941 gcgcacgcgc tcgagcaggt gcggcgccgc cgcggccagg gcctcgcgct ggcgctcacg
     9001 caggtcggcg ctcacgtcga ggatcaaata gcggcccggc aacgcgtcac ggcgctcgag
     9061 ctcgagcagc aggtcgcacg ccatcgcgcc tgaacccgcg ccgagttcca gcacttcggc
     9121 acggggcccc agctcgcgca gcacctcggc gcactgcacg gccaggcaac ggctgaacac
     9181 cggcgccacc tcgggcgccg tgacgaaatc gccggccgcg ccgaacttgc gcgctccggc
     9241 gctgtagtac ccgagaccgg gctcgtagag cgccatgcgc atgaagtcgt cgaacgacat
     9301 ccagccgccc gcgccagcga tcgcctcggc gatgcgtgcc aagagccgct caccgtgcgc
     9361 ggcttcctcg gcggacaact cgggcagtcc ggtcgcgacg gtcatcgtgg cctgaggtat
     9421 cgtgcggcga gcatcacgat cacgcggagg gcatccgcgg cggccgcgcg atcacagcgc
     9481 gtccaccgcg gcagccggga tgagcaggac gcctggacag ggaccactcg aaggaaaggt
     9541 ggcattgatc accggcgggg cacgccgtct cggcgccgcg atcgcccgcc ggctgcacga
     9601 cgcgggcgcg cgcgtcgcgg tccactaccg gtcctccgcg acggacgcga cggcgctggt
     9661 ggcggaattt catgccgtgc gcgccgattc ggcggtcgcc gtcgccggcg acctgctgga
     9721 caccgatgcg atcccatcga tcgtcgagga ggccacccgg cgcttcggcc ggctcgacgt
     9781 gctggtcaac aatgcatcga gcttttacgc gaccccgctc ggctcgatca cgcccgggca
     9841 gttcgacgac ctcatcggca cgaacctgcg ggcgccgctg ctgctggcgc aggcggccgc
     9901 cccggcgttg cgccaggccc gcggcctgat cctcaacatg gcggacatcc acggacaacg
     9961 gccgctgcgc gaccatgccg tgtactcggc tgcgaaggcc gggctgatca tgctgacgcg
    10021 ctcgctcgcg cgcgaactcg ggccggaagt gcgcgtcaat gccatcgcac ccgggccggt
    10081 gctctggccg gagagcggca tggccgccga cctgcaggcg gaaatcgtcg gcaagaccgc
    10141 gctccttcgc agcggttcgc cggaagacat cgccgccgcc gcgtatttct ttgccgccga
    10201 agcgccgtac gtcaccggcc aggtgctcgc gatcgacggc ggtcgcagcc tctgatcgca
    10261 cgtcagcgcg actcggcgag ctcgatacga cgcaagtcgt gcgccggtgc gcgaagctgc
    10321 atctcacgcc agagttccgc gagcgtgcgt cgcgccgtgg ggtgctcgac ctcaggcgcg
    10381 atctcggcgg ccgggccgag catgtacggc cggcgcagca ggtccggccg tggcaacgtg
    10441 aatccccgtt cggcgcagac gaggtcaccg tagagcagca cgtcgaggtc catcgcacgc
    10501 ggcgcccact tcggcgcgtc gcgctcacgc ccgcaggcgg cctccgcctc gtgcaggcgc
    10561 gcgagcacct gctgcaccgg caggtcggtg tcgaagcgca cgaccaggtt cacgaagtcg
    10621 tcgccctcga acccgacggc ggcgttggcg tacgcgggcg acacctgcag gccgggaaag
    10681 tgccgtcgca gcgcctcgag cgcgcgtcgc aggttggcga tcggcgcgac gttgctgccc
    10741 gccgcgacca ggacgtcgcg agtcacgtcg ctggcgcgct cacgtgccgg cacgcgagtc
    10801 gggcggtagg taatcctcgc ggcgccgctc gatcacgatg cccacgtcgc gcgcaccgcg
    10861 cagcgcgccc tgcttgttga ggcgcacctt caccgcaggc acgccgaact cggtgatcac
    10921 cagttgcgcg atgcggtcgg tcagcgtctc gacgagctgg aactgcgact gctcgatgaa
    10981 ctgctgcagc cgtttcgaca ccctcttgta gtcgagcgtg tcgtcgatgt ggtcgctcgc
    11041 ggcagcgcgg cggatgtcgg cggccatctc caggtcgatc acgaccttct gcctgacctt
    11101 acgttcccag tcccagatgc cgatgatgca ttcggcggtc aggccgccca ggaagatcgt
    11161 gtctgtcatg tcaccccgtc gcgccggcgc cgtgttcgtc gttctgccgg ttcaaccgcg
    11221 cgcggcgggc gcgggtggca caagttcggt catcggccag cgggcccgcg cgcggatcga
    11281 gcggtcgtcg cgctcgcccg ccgcgagtcg caggtggccg agcagtgcga tcatcgccgc
    11341 gttgtccgtg cagaattcgg cgcgaggata gacgacgcgc acgccttcgc gctcgcccat
    11401 ctcgagcatg cgggcgcgca gccgccggtt cgcccccacc ccgccggcga cgacgagcgt
    11461 acggctgtcc gtcgcctcga gcgcacgtcg gcacttgatg accagcgtat cgacgaccgc
    11521 ctcctcgaaa ccgcgagcca cgtcggcccg tgcctgcgcg tcgagctcac cgacgtggtt
    11581 gcgcaacgcc acgacgaccg ccgtcttgag gccgctgaag ctgaagtcga gccccggccg
    11641 ctcggtcagc ggtcgcggga agcgaaagcg gtcgggccgc ccccgctcgg cgagcgccgc
    11701 cagctgtggc ccgccagggt agccaaggcc gagcagcttg gccgtcttgt cgaaggcctc
    11761 gccggccgca tcgtccagcg aactgccgag cagttcgtac tcgccgatcc ggcggacgcg
    11821 cgccagcagg gtatgccccc ccgacaccag caaggccacg aacgggaatt cgggcggctt
    11881 gggctcgagc agcggcgcca gcaggtggcc ttccatgtgg tgcaccccga cgctgggcac
    11941 gcgccaggcg tacgccagcc cctgagcgac ggcggccccc accagcaagg caccgaccag
    12001 gcccgggccg ctcgtgtaag cgatgccgtc gatgcccgag cgaccgccgg cgacggcgag
    12061 ggttttttcg atcagtggca ataacttgcg caggtggtcg cgcgacgcca gctcgggcac
    12121 gacgccgccg taccgggcat gcagctcgac ctggctgtag agctcgtgcg ccagcaggcc
    12181 gcgctcgccg tcgtagacgg ccgccgcagt ctcgtcgcag gaggtctcga tcccgagtac
    12241 gcgcatcggt gccacaccac ccggcaggta aaagacgaag ccccgtccac ccgcgtcggc
    12301 tttgcaattg gccgccgcgg tctctagaat tcgcggtccc ttgccggccg gtgcccacag
    12361 ggcccgttcg gctgctcagc aacccttggc gactggctgc agacacgacg gccggccagc
    12421 cgttttgacg aggtttcgat gcccagcgtc cgcatccgtg agaacgaata tttcgatgcc
    12481 gccctgcgtc gcttcaagcg cgcgtgcgaa aaggccggcg tgctgacgga actccgccgt
    12541 cgcgaattct acgagaagcc gacgcaggaa cgtaagcgca agaaggctgc cgccgtgaag
    12601 cgccaccaga agcgcagcac gcgcgacttc acgtcggcgc cccgcgcccg cacctactga
    12661 cgacgggcga ccaaccgcgc gagcgcgaag cccctccccc gcgctcgtcg cgggccccca
    12721 cgaagcggcg tcccggtcca gtgatcgagc gccgcttcgt cgtttctgga ctgcgcaggt
    12781 cgccctttct ctttctttcg acaccacgac ggacaactcc atgagcagca gcaccgcacc
    12841 gtccctcaag gagcgcatca ccgaggacat gaagagcgcg atgcgcggcg gcgacaaaga
    12901 acgcctcggc acgatccgca tgctgcaggc cgcgatcaag cagcgcgagg tcgacgagcg
    12961 catcacgctc gacgacgcac aggtgctcgc cgtgatcgag aagatggtca agcagcgcaa
    13021 ggagtcgatc gtgcagttcg agcagggcgg ccgtgccgac ctggcggcga aggagaaggc
    13081 cgagatcgag cagttgcagg cctacctgcc ggcgcagctc agcgatgcgg cggtcgacgc
    13141 gttgatccgc gaggcgatcg cggcgacggg cgccagttcg atcaaggaca tgggcaaggt
    13201 gatgggcgcg gtcaaggcga aggcggccgg ccgcgccgac atgggcgcgg tgagcgcccg
    13261 catcaaggcg gcgctcggcg cctgatcgtc atgcgttcgc tcgccacgac cggcccgaga
    13321 agaaccggag cagtgccggg cgcccgatcc gggtgttcca ccccggctcc gaaagccgac
    13381 atgtcgccgg gtggagcaac ccggatcgag cgctccacgc acggcgcctg acactgcgcc
    13441 ggagtgcctc gcctccatgc aggcttgagg agacgcggcg acatgtcggt tttcggagct
    13501 gcggaaaatc atgcg
//
LOCUS       DASPFR010000285         2787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1422019, whole genome shotgun sequence.
ACCESSION   DASPFR010000285 DASPFR010000000
VERSION     DASPFR010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422019"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VD737_07610"
     CDS             <1..1182
                     /locus_tag="VD737_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXW10463.1"
                     /translation="GEAQETVDFFRHYADDFEQHAGYEHALPDDPLEHVVSRNRSVMR
                     PYGAWVVIAPFNFPLALAGGPAAAALVTGNTVVVKGSTETPWAGRLLADCVRDAGLPS
                     GVFQYLSGPGREVGEALATHPATAGITFTGSVRVGRRLLTQMAGGPWPRPCIAEMGGK
                     NPCIVTARANLDDAAAGIVRSAYGMDGQKCSALSRLYVERGVADELIDRLRAQMAAIR
                     IGDPTRRENWLGTVISANAARNHARFVEELRSGGATILAGGRELREGDLAHGHYVDPV
                     LAEAPLGHPLWQQEMFVPILMLQRVANRDEAMQHANDSTLGLTAGFYGAAEEVPWFQE
                     HIEAGVTYANRKQGATTGAWPGYQPFGGWKGSGSTGKAIASFYYLAQYLREQSRTVVD
                     P"
     gene            1179..2033
                     /locus_tag="VD737_07615"
     CDS             1179..2033
                     /locus_tag="VD737_07615"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXW10464.1"
                     /translation="MSSPKLVSLREAIATHVRDGQCLALEGFTHLIPFAAGHEIVRQG
                     RGDLHLVRMTPDLVYDQMIGAGCARRLTFSWGGNPGVGSLHRLRDAVEHGWPRPLELD
                     EHTHAGVAAAYCAGAARLPFATLRGYLGTDLPSVNPRVRSVGCPFTGERLAAVPALNP
                     DVTILHAQRADRRGNVAIHGIVGAQREAALAATTLIVTVEEIVDELPPAMNSIVLPHW
                     IVTAVAHCPGGAYPSYVQGHYARDNAFYQRWDAIARDRDTFRAWMDDHVLGCRDHAAF
                     LARLRAAA"
     gene            2030..>2787
                     /locus_tag="VD737_07620"
     CDS             2030..>2787
                     /locus_tag="VD737_07620"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXW10465.1"
                     /translation="MSWTRDEMMTVAAARQLWDGCVCFVGIGLPSAACNLARLTHAPD
                     LVLVYESGTVGTRPDVLPLSIGDGELAETAACVISLPEVFTYLLQAGRVDVGFLGAAQ
                     IDRHGNLNSTVIGPYASPRTRLPGAGGATEIAAHARRTFVMLKATPRSLVERLDFRTS
                     AGYLDGHGARSRTTMRGAGPAVVITDYGLLTPEPETDELRLAALFPGVTIDDARAATG
                     WPLAVATEVAALEPPSPLELDTLRALHARTREAHR"
BASE COUNT          395 a         1019 c          974 g          399 t
ORIGIN      
        1 ggcgaggcgc aggagaccgt cgacttcttc cgccactatg ccgacgactt cgagcagcac
       61 gccggctacg agcacgcgtt gcccgatgat ccgctcgagc acgtggtgtc gcgcaaccgc
      121 agcgtgatgc gaccgtacgg cgcgtgggtc gtgatcgcgc cgttcaactt cccgctcgcg
      181 ctcgcgggtg gcccggcggc cgcggcgctc gtcaccggca acacggtcgt ggtcaagggt
      241 tccaccgaga cgccctgggc gggccggctg ctcgccgact gcgtccgtga cgcagggctg
      301 ccgtccggcg tgttccagta cctgtcgggc ccggggcgcg aggtgggcga agcgctcgcc
      361 acacatcccg cgacggccgg catcacgttc actggctccg tccgcgtcgg ccggcgcctg
      421 ctcacgcaga tggcaggggg gccgtggcca cgtccctgca tcgccgagat gggtggcaag
      481 aacccctgca tcgtcaccgc gcgcgccaac ctcgacgacg cggccgccgg catcgtgcgt
      541 tccgcgtacg gcatggacgg gcagaagtgt tccgcgctgt cgcgcctgta cgtggagcgc
      601 ggcgtcgccg acgaactgat cgaccgcctc cgcgcacaga tggccgcgat ccggatcggc
      661 gacccgacgc ggcgtgaaaa ctggctcggc acggtgatca gcgccaacgc cgcccgcaac
      721 cacgcccgct tcgtcgagga gctgcgcagc ggcggcgcga cgatcctcgc gggcggacgc
      781 gagctgcgcg aaggcgacct cgcgcacggc cactacgtcg atccggtgct ggccgaggca
      841 ccgctcggcc acccgctgtg gcagcaggag atgttcgtgc cgatcctgat gctgcagcgg
      901 gtcgcgaacc gcgacgaggc gatgcagcac gccaacgact cgacgctcgg gctcacggcg
      961 ggcttctacg gcgccgcgga agaagtgccg tggttccagg agcacatcga ggccggtgtc
     1021 acctacgcca atcgcaagca gggggccacc acgggcgcgt ggcccggtta ccagccgttc
     1081 ggcggctgga agggttccgg ctcgaccggc aaggccatcg cctcgttcta ttacctggcg
     1141 caatacctgc gcgagcagtc gcgcaccgtg gtggatccat gagctcgccc aagctcgtgt
     1201 cgctgcgcga agcgatcgcc acgcacgtgc gcgacgggca gtgcctggcg ctggagggct
     1261 tcacgcacct gattccgttc gcggccggcc acgagatcgt gcgccagggc cgcggcgacc
     1321 tgcacctggt gcgcatgacg cccgacctgg tctacgacca gatgatcggt gccggttgcg
     1381 cgcggcgcct cacgttctcg tggggtggca atccgggcgt gggctcgctg caccggctgc
     1441 gcgatgccgt cgagcacggc tggccccgcc cgctcgagct cgacgagcac acgcacgcgg
     1501 gtgtggccgc ggcgtattgc gcaggcgccg cgcgcctgcc gtttgcgacg cttcgcggct
     1561 acctcgggac cgaccttccc agcgtcaacc cgcgtgtgcg cagcgtcggc tgcccgttca
     1621 cgggagagcg gctggccgcc gtcccggcgc tgaaccccga cgtgacgata ctgcacgcgc
     1681 agcgcgcgga ccgtcgcggc aacgtcgcga tccacggcat cgtcggcgcg cagcgcgaag
     1741 ccgcgctcgc ggccacgacc ctgatcgtca ccgtcgagga gatcgtggac gaactgccgc
     1801 ccgcgatgaa ctcgatcgtc ctgccgcatt ggatcgtcac ggccgtcgcg cactgcccgg
     1861 gtggcgcata cccgtcgtac gtgcagggcc actacgcgcg tgacaacgcg ttctaccagc
     1921 gctgggacgc gatcgcacgc gaccgtgaca cgttccgcgc ctggatggac gaccacgtgc
     1981 tcgggtgccg cgaccacgcg gcgttcctgg cgcgcctgcg ggccgccgca tgagctggac
     2041 gcgcgacgag atgatgacgg tcgcggccgc gcgccagttg tgggacggct gcgtttgctt
     2101 cgtcggcatc ggcctgccga gcgcggcctg caacctcgcg cggctgacgc acgcacccga
     2161 cctcgtgctc gtctacgaat ccggcacggt gggcacgcga cccgacgtgc tgccgctctc
     2221 gatcggcgac ggtgaactcg ccgagaccgc cgcctgcgtc atatcgctgc ccgaggtgtt
     2281 cacctatctg ctgcaggcgg gtcgcgtcga cgtcggcttc ctgggtgccg cgcagatcga
     2341 ccggcacggc aacctgaaca gcacggtgat cggaccctac gcctcgcccc ggacgcgcct
     2401 gccgggggcc ggtggcgcga ccgagatcgc ggcgcacgcg cgtcgcacgt tcgtgatgct
     2461 caaggcgacg ccgcgcagcc tcgtcgagcg cctcgatttc cgtaccagtg ccggctacct
     2521 cgacggtcac ggcgcccggt cgcgaaccac gatgcgcggg gcgggccccg ccgtggtgat
     2581 cacggactac ggactgctga cacccgagcc ggagaccgac gaactgcgcc tggccgcgct
     2641 gtttcccggc gtcacgatcg acgacgcccg tgccgccacc ggctggcccc tcgcggtcgc
     2701 aacagaggtc gcggcgctcg agccaccgag tcccctcgaa ttggatacgc tgcgcgcact
     2761 gcacgcgcgc acgcgcgagg cgcatcg
//
LOCUS       DASPFR010000286         3933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_325047, whole genome shotgun sequence.
ACCESSION   DASPFR010000286 DASPFR010000000
VERSION     DASPFR010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3933
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325047"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3933)
                     /locus_tag="VD737_07625"
     CDS             complement(<1..>3933)
                     /locus_tag="VD737_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HXW10466.1"
                     /translation="KLRTFLFAAENVAEEGEPLDLVPRERFSGVPEFYLLHVTSELDA
                     NDDKPGRAAKLDGHDDRLETGPDAALNLTRTGTVEMWFNVQGAGDVLDFGAAAWMSLL
                     HKGGNTAGTRQYSLWLNENGRLVFASNDTNQVQSAINLIRPNEWYHVTGVMDRDHGTM
                     RLYLNGELVGTNTIATTNAPGSNTSLYIGDRHGTDTLGGDVVRFKGSLDEVRVWNVVR
                     TDSQIRHAHQRTVEADAQGLAAYWRFSEPEGTTFNDSADLTPNQIDERQLALMLDLEP
                     GAPLGVDNQAPSVAHADGRALILPFGPGQYQIKFVGTLGQTLHVEGDDETPAFSAVSL
                     GGQPVVMALGDIDGDSFDDAVVSIRDGVPDGQGGFRHYARVAFGTADGLDPAKYGLPV
                     TLQLPSPILTGPAGLRATISGVGDVNADGFDDIAVSVPSNTPGESKVYLVFGRADWSQ
                     DNPEQDAGLVGEYFFLGGSVLAFPDFDDLEPDLERIDSQINFPNTAGAFSGVSDADTF
                     AVRWTGQINIETPGTYTFFLGSDDGSRLYIDNSNEPVVDNGGLHPFQEKSGLITLDAG
                     LHEIRVEYFENFGFAGVQLSWDPPGAAGKQLVPGSVLFRNSRDVLSVASEGDVLLEGF
                     SGAVQAIGAGDVTPVIGQGLNAQVFDLVPTGIAPVNELLSFDGANDVVTIASTPSLTP
                     ASQVTIEARIRVDAFTNQFMTVVTKGDATPFGRSYELFVRDDGGLLLVSSDGSDAGQF
                     LNTAAGLVTQGVWYEVAAVVDRGAGTQKIYLDGAEVGSLSVPTTDLAAHDSPLLFGNW
                     LESFATVSPFQGRIDEVAVWGTARTQGEIVADFEGIDPAAAGLAGHWKLNEASGDAVL
                     DASPNGNDGVLGDGIDLSRNPARVAIFTQFPDFETLTPAFEFVDSNIIHFLEAENFGG
                     SELDSQFAVVWTGHIRIDTAGDTTFALGVDDRARLFIDGAETPLIDFFTPPTGTVDLE
                     AGLHSIRVEYLAGAAPSTIFLFWDSQDDADTDLSLIDNANLVRVDATFTDPTAAGTDD
                     LLIRDAGGVRVVHGRVAWEDTLTGAAIDDTFTTGGAIAALGDINRDGRDDVAVIDGTE
                     LRIYRGGGALPEQLLEQHSITGLPAGVKVYRAGDIDGDAAQDILVTGANGSTLIFGGD
                     LEDDQALGDLVDDDLAIELGKGDWRPIGDFDGPGPDGNTFDDLGAAVLLTTDKLNETG
                     QTEHQAVSVFLGGARGTLVEAFAVADIVFEPGRASFTDPGEATPDATFFGSLGLRTGT
                     DGESRALLGISGPAGDALRLYDGERLGLAEGTEETPGLENEPDLWRFE"
BASE COUNT          553 a         1321 c         1409 g          650 t
ORIGIN      
        1 gctcgaagcg ccagaggtcc ggctcgtttt cgagccccgg cgtttcctcg gtgccttcgg
       61 ccagcccgag ccgctcgccg tcgtagagcc gcagcgcatc gccggccgga ccgctgatgc
      121 caagcagcgc gcgcgattcg ccgtcggttc cggtgcgcag cccgagcgag ccgaagaagg
      181 tcgcatccgg cgtggcctct cccgggtcgg tgaagctggc gcggccgggc tcgaacacga
      241 tgtcggctac ggcgaacgcc tccacgagcg tgccgcgcgc gccgcccagg aacacgctca
      301 ccgcctggtg ctcggtctgg ccggtttcgt tcagcttgtc ggtggtgagc aggaccgcag
      361 cgccgagatc atcgaaggtg ttgccgtcgg ggcccgggcc gtcgaagtcg ccgatcggcc
      421 gccagtcgcc tttaccgagc tcgatggcga ggtcgtcgtc caccaggtcg ccgagcgcct
      481 ggtcgtcttc gaggtcgccg ccgaagatga gggtgctgcc gttcgccccg gtgacgagga
      541 tgtcctgcgc cgcgtcgccg tcgatgtcgc ccgccctgta caccttcacg cccgcgggca
      601 ggccggtaat gctgtgctgc tcgaggagct gctcaggcag cgcgcccccg ccgcgataga
      661 tgcgcagctc ggtcccgtcg atgaccgcga cgtcgtcgcg gccgtcgcgg ttgatgtcgc
      721 cgagcgccgc gatcgcgccg ccggtcgtga acgtgtcgtc gatcgccgcg ccggtgaggg
      781 tatcttccca ggccacgcgg ccgtgcacca cgcgtacgcc gccagcgtcc cggatcagga
      841 ggtcgtcggt gcccgcggcg gtcgggtcgg tgaacgtcgc atcgacgcgt accaggttgg
      901 cgttgtcgat caggctgaga tcggtgtccg cgtcgtcctg cgaatcccag aacaggaata
      961 tggtcgacgg cgcagcgccc gccaggtact cgacgcggat gctgtgcagt cccgcctcga
     1021 ggtcgaccgt gccggtgggc ggcgtgaaga aatcgatcag cggggtctcc gcgccgtcga
     1081 tgaagagccg cgcgcggtcg tcgacgccga gcgcgaacgt ggtgtcgccc gccgtatcga
     1141 tccggatatg gcctgtccac accacggcga actggctgtc gagctccgag ccgccgaaat
     1201 tctccgcttc gaggaaatgg atgatgttgc tgtcgacgaa ctcgaacgcg ggcgtgagcg
     1261 tctcgaagtc cgggaactgg gtgaagatgg cgacgcgcgc cgggttgcgc gacaaatcga
     1321 tgccgtcgcc gagcacgccg tcgttgccgt tcggcgaggc gtcgagcacc gcgtcgccgc
     1381 tcgcctcgtt cagcttccag tggcccgcga gcccggccgc cgccgggtcg atgccttcga
     1441 agtcggcgac gatctcgccc tgcgtgcgcg ccgtgcccca caccgcgact tcgtcgatcc
     1501 ggccctggaa cggagagacg gtggcgaagc tttccagcca gttgccgaac agcagcgggg
     1561 agtcgtgcgc cgccaggtcg gtggtcggga cagacaagct gcccacctcg gccccgtcaa
     1621 ggtagatctt ctgcgtgccg gcgccgcggt cgacgaccgc cgcgacctcg taccagactc
     1681 cctgggtcac caagccggcc gcagtgttga ggaactgtcc tgcgtcgctg ccgtcgctgc
     1741 ttacgagaag caggccacca tcgtcgcgca caaagagttc atagctgcgg ccgaagggcg
     1801 tcgcgtcgcc cttcgtcacc acggtcatga actggttggt aaaggcgtcc accctgatgc
     1861 gggcctcgat ggtcacctgg ctcgcgggcg tcaggctggg ggtgctcgcg atcgtgacga
     1921 cgtcgttggc gccgtcgaaa ctgaggagct cgttgaccgg ggcgatgccg gtcgggacga
     1981 ggtcgaagac ctgggcgttc aggccctggc cgatgaccgg ggtgacgtcg cccgcgccga
     2041 tcgcctgcac cgcgccgctg aagccctcga ggagcacgtc gccctcgctc gcgacgctga
     2101 ggacgtcgcg cgagttgcgg aacagcacgc tccccggcac gagctgcttg ccggccgctc
     2161 ccggcggatc ccaggagagc tgcacgccgg cgaagccgaa gttctcgaag tactcgacgc
     2221 ggatttcgtg caggccggcg tcgagcgtga tcaggcccga cttctcctgg aatgggtgca
     2281 ggccgccgtt gtcgacgacg ggctcgttgc tgttgtcgat atacaggcgc gagccgtcgt
     2341 cgctgccgag gaagaaggtg tacgtcccgg gcgtctcgat gttgatctgg ccggtccagc
     2401 gcaccgcgaa ggtgtcggcg tccgacacgc cggagaatgc gccggcggtg ttcgggaagt
     2461 tgatctgcga gtcgatgcgc tcgaggtcgg gctcgaggtc gtcgaagtcc gggaaggcga
     2521 ggaccgagcc gccgaggaag aagtactcgc cgaccaggcc cgcgtcctgc tcggggttgt
     2581 cctggctcca gtcggcgcgg ccgaagacga ggtagacctt gctctcgccc ggggtgttgc
     2641 tcggcaccga gaccgcgatg tcgtcgaagc cgtcggcgtt gacgtcaccc acgccgctga
     2701 tcgtggcgcg cagcccggct ggcccggtca ggatcggcga cggcagctgc agcgtgaccg
     2761 gcaggccgta cttggcggga tcgaggccgt ccgccgtgcc gaaggcgacg cgcgcgtagt
     2821 ggcggaagcc gccctggccg tcgggcacgc cgtcgcggat cgagaccacg gcgtcgtcga
     2881 agctgtcgcc atcgatgtcg cccagcgcca tcaccaccgg ctggccgccg aggctcaccg
     2941 cggagaaggc cggcgtctcg tcgtcgccct cgacgtgcag ggtctggccc agcgtgccga
     3001 cgaacttgat ctggtactgc cccggcccga agggcaggat gagcgcgcgg ccgtcggcgt
     3061 gcgccacgct cggcgcctgg ttgtcgacgc ccagtggcgc gcccggctcg aggtcgagca
     3121 tgagcgcgag ctggcgctcg tcgatctggt tcggcgtcag gtcggcgctg tcgttgaagg
     3181 tcgtgccctc gggctcgctg aagcgccagt acgccgcgag cccctgcgca tccgcctcca
     3241 ccgtgcgctg gtgggcgtgc cggatctggc tgtcggtgcg caccacgttc cacacgcgca
     3301 cctcgtcgag cgagcccttg aaccggacca cgtcgccgcc cagcgtgtcg gtgccgtgcc
     3361 tgtcgccgat gtagagcgaa gtattcgagc ccggcgcgtt ggtggtggcg atggtgttcg
     3421 tgccgacgag ctcgccgttc aggtacaggc gcatcgtccc gtggtcgcgg tccatcacgc
     3481 cggtgacgtg ataccactcg ttcgggcgga tgaggttgat cgccgactgc acctggttcg
     3541 tgtcgttcga ggcgaagacg agccggccgt tctcgttcaa ccagagcgag tattgacgcg
     3601 tgccggccgt gttgccgccc ttatgcagca gcgacatcca ggccgcggcg ccgaagtcga
     3661 gcacatcgcc tgcgccctgc acgttgaacc acatctccac ggtgccggtg cgcgtgaggt
     3721 tgagcgcggc atccggaccg gtctcgaggc ggtcgtcgtg gccgtcgagc ttggcggcgc
     3781 ggccgggctt gtcgtcgttc gcgtcgagct cgctcgtgac gtgcagcagg tagaactcgg
     3841 gcacgccgga gaagcgctcg cgcggcacga ggtcgagcgg ctcgccttcc tcggcgacgt
     3901 tctcggcggc gaacaggaac gtgcgcagct tct
//
LOCUS       DASPFR010000287         3124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1179865, whole genome shotgun sequence.
ACCESSION   DASPFR010000287 DASPFR010000000
VERSION     DASPFR010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179865"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VD737_07630"
     CDS             <1..409
                     /locus_tag="VD737_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10467.1"
                     /translation="TTDAAPPEAPQLPLRQALARNDLRDFTPRAPTLLCGGHDDPVVF
                     WLNTQAMQSYWATQAGGGAVTVLDVDAAPSSSDDPYGDLKQRFAVAKDLVAAQAIAAG
                     ATDGGWEAVLETYHAGLVAPFCLEAVQEFFETH"
     gene            complement(485..1315)
                     /locus_tag="VD737_07635"
     CDS             complement(485..1315)
                     /locus_tag="VD737_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10468.1"
                     /translation="MEVTRNKLNVAICACLAAGALASAGAVAHEYDQDPATSASATPT
                     DSGVSSSSTTSPSTTYTPGTSSSAYTSDSSMDADQSTTSGTTSTTTGTTSDTTGTSAD
                     TSSGMSTGTSADTSSGSTTSSTQQDTGYTSPRSDADQSRAASSRSGSAAGPDAVVWYI
                     LPVAYEVQSNQMSKGCWVRLYSGDEFEGRSINIVGPANIREMQSPYGTGMNNWESAEV
                     GPNATVTTYDDENFGSRNATLKAGQRYPDLDDSKLGLFDDIESMKVTCRDSAGTTSSN
                     "
     gene            complement(1553..2158)
                     /locus_tag="VD737_07640"
     CDS             complement(1553..2158)
                     /locus_tag="VD737_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10469.1"
                     /translation="MSPFGRTRFAVVPLLLVAATASARDESAAGDRAWTATVYGARMS
                     SETGWEDILIDPVEAQYVDVFLLAGALSRPYVSLNDGALTLEAEGQVVRYFGDQDHWE
                     FNALPIILRWHRFPWSHRVDTSAAFGLGVSYATELPPVEVAIEGETAQWLIYWLLEAT
                     AGPVDSPWSFTLRMHHRSVAYGLMAEEGGMNAVGVGVRYRF"
     gene            complement(2205..>3124)
                     /locus_tag="VD737_07645"
     CDS             complement(2205..>3124)
                     /locus_tag="VD737_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10470.1"
                     /translation="SAAPVRATPIVLLPRRDVRVWSSLVPPADPQNGSSTARAVAEFI
                     HEHGAAFFDEIVEGTRLLPTHVEVALAELVALGLVNADSYAGLRVLLLPVDRRRRPGD
                     TRRPRGRVALFGMQDAGRWTLVRRAPRSRAPAGAQATPDVQAAAVEHVARVLLERWGV
                     MFWKLYQREAGWLPPWRDLLMVYRRLEARGDIRGGRFVAGFSGEQYALPAAIGAMRES
                     RRREPQGEWVSISAADPLNVVGLLTPGARVPSLTRNRVLYRDGVPVATLSGGEAAVHE
                     HFDDASEWDVRNRLLRRPALRKVRVTPEP"
BASE COUNT          472 a         1105 c         1071 g          476 t
ORIGIN      
        1 caccaccgac gccgctccgc ccgaggcccc gcaactgccg ctgcggcagg cgctggcacg
       61 caacgacctg cgcgacttca cgcctcgggc cccgacattg ctgtgcggtg gtcacgacga
      121 ccccgtcgtc ttctggctca acactcaggc gatgcagtcg tactgggcca cacaggccgg
      181 gggaggcgcc gtcaccgtgc tcgacgtcga cgccgcaccg tcgagcagcg acgatccgta
      241 cggcgacctc aagcagcgct tcgccgttgc gaaagacctc gtcgctgcgc aggccatcgc
      301 cgccggcgcc accgacggcg ggtgggaagc cgtcctcgag acgtaccacg cgggcctcgt
      361 cgcgccgttc tgtctcgagg ccgtgcagga gtttttcgag acgcactgac ggcctgcgat
      421 cgatccatcc atcttggcga gcgaggccgc gccggaggcg cgacctcgtc cgcgagcggg
      481 tacgtcagtt gctggacgtc gtgccggcgg aatcgcggca cgtgaccttc atcgactcga
      541 tgtcgtcgaa caggccgagc ttggaatcgt ccaggtccgg atagcgctgg cccgccttca
      601 gcgtcgcgtt gcgcgacccg aagttctcgt cgtcgtaggt cgtcaccgtg gcgttcggcc
      661 cgacctcggc gctctcccag ttgttcatgc cggtgccgta gggcgactgc atctcgcgga
      721 tgttcgccgg acccacgatg ttgatcgacc ggccctcgaa ctcgtcaccc gagtagaggc
      781 ggacccagca tcccttggac atctggttgc tctggacctc gtaggcgacc ggcaggatgt
      841 accagaccac ggcgtccggg ccggcagccg agccgctgcg cgaactcgcg gcgcgcgact
      901 ggtcggcgtc ggagcgtggc gaggtgtagc ccgtgtcctg ctgcgtggac gacgtcgtcg
      961 agccgctcga ggtgtccgcg ctcgtaccgg tagacatgcc ggagctggtg tccgcactcg
     1021 tgccggtggt gtcggacgta gtgcccgtgg tggtcgacgt ggtccccgag gtagtcgact
     1081 ggtcggcgtc catcgacgag tccgacgtat atgccgacga actcgtgccc ggcgtatacg
     1141 tcgtggacgg gctggtcgta gaactgctcg acacgcccga gtcagtgggc gtcgccgagg
     1201 cgctggtcgc cgggtcctgg tcgtactcgt gcgccacggc accggccgag gccagggcac
     1261 ccgcggcgag gcatgcacag atcgcgacgt tgagcttgtt tcgcgttacc tccattcttt
     1321 gtcctccttc ctgcgtgcta cggtcgaagc gtaagcgagg cgtgtcgttc aacgcctgtc
     1381 tatatactga tccggaacgc agggaggggt tcctcggcgg tggcgcggga ccattgcacc
     1441 gattcgcgtc ggacggcgcg cacaacgagc cgaggttaac gtgcaaccgc gggcgcgcgg
     1501 gcttcgagct gggtgcgcgc aggatccgtg agtcgcgcag caggactcat ggctagaacc
     1561 ggtagcgcac gccgacgccg accgcgttca tgccgccctc ctcggccatc aaaccgtacg
     1621 ccacggagcg atggtgcatg cgcagcgtga acgaccacgg tgaatcgacc gggccggccg
     1681 tggcctcgag caaccagtag atcaaccact gcgcagtctc gccttcgatc gcgacttcga
     1741 cgggtggcaa ctcagtggcg tacgacacgc cgaggccgaa cgcggccgac gtatcgaccc
     1801 ggtgactcca cgggaaccgg tgccagcgga gaatgatcgg cagggcgttg aattcccagt
     1861 ggtcctggtc gccgaaatag cggacgacct ggccttcggc ttccagcgtc agcgcgccgt
     1921 cgttcaggct cacgtagggt cgcgacaacg cgccggcgag caggaagacg tcgacgtact
     1981 gcgcctcgac gggatcgatc aggatgtcct cccaaccggt ctcgctggac atgcgggcgc
     2041 cgtacacggt cgcggtccac gcgcgatcgc ccgcggccga ttcatccctg gccgacgcgg
     2101 tcgccgccac gagcagcaat ggcaccacgg cgaaccgcgt tcgaccgaac gggctcatcg
     2161 cgcggaggca catccgcggg ctttcgtgtc ggtcaggggc agtttcacgg ctcgggcgtg
     2221 acccgcacct tgcgcagcgc cggacgtcgc agcaaccggt tgcggacgtc ccactccgac
     2281 gcgtcgtcga agtgctcgtg gacggccgct tcgccaccgc tcagggtcgc gaccggcacc
     2341 ccgtcgcgat acagcacgcg gttgcgcgtc agcgagggca cgcgggcgcc gggcgtcagc
     2401 aggccaacga cgttgagcgg gtcggccgca ctgatcgaca cccactctcc ctggggttcg
     2461 cgtcggcggc tttcccgcat cgcgccgatc gccgcgggca acgcgtactg ctcgcccgag
     2521 aatccggcga cgaagcgacc gccacggatg tccccgcgag cctcgaggcg gcgatacacc
     2581 atcagcaggt cgcgccacgg cggcagccag cctgcttcgc gctggtacag cttccagaac
     2641 atcacgcccc agcgctcgag cagcacgcgc gcgacgtgct ccacggcggc cgcctggacg
     2701 tccggcgtcg cttgtgcgcc ggcgggcgcg cgactgcgtg gcgcacgccg caccagcgtc
     2761 cagcgacccg cgtcctgcat gccgaacagc gccacgcggc ctcgcggacg ccgcgtgtcg
     2821 cccgggcgcc gccggcggtc gactggcagc agcagcacgc gcaggcccgc gtagctgtcg
     2881 gcgttgacga ggccgagcgc cacgagctcc gccagcgcga cctcgacgtg cgtcggcagc
     2941 agccgcgtgc cctcgacgat ctcgtcgaag aaggccgccc cgtgctcgtg gatgaactcg
     3001 gccaccgcgc gcgccgtgct gctgccgttc tgcggatcgg cgggcggcac cagcgacgac
     3061 cagacgcgca cgtcgcggcg cggcagcagc acgatcggcg tcgcgcgcac cggcgccgcg
     3121 ctac
//
LOCUS       DASPFR010000288         5687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1669731, whole genome shotgun sequence.
ACCESSION   DASPFR010000288 DASPFR010000000
VERSION     DASPFR010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669731"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1830
                     /gene="acnA"
                     /locus_tag="VD737_07650"
     CDS             <1..1830
                     /gene="acnA"
                     /locus_tag="VD737_07650"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HXW10471.1"
                     /translation="GAGVTATDLALNVTQRLREIDVGGRFVEFCGPGVAEITAGTRAA
                     VANMAPEFGGATGYFPIDERTLEYLRATGRDDDHVARVGRYARQQQLWFDPQARPDYD
                     RLVEFDLDSVRPSVAGPRRPQDRIDRSAAPALAGDDRQPRPIAIAAITSCTNTSDARL
                     LVAAGLLARNAIERGLRVPRWVKTSLAPGSPTARIVLQRAGVLGALERLGFAIVAHGC
                     TTCIGNSGPLTAVMETMVESGARPVAVLSGNRNFPGRVHRRITDAYLASPPLVVAYAL
                     AGDFDRRIEAEPLGHSPTGQAVYLHELWPPDEEVDAVIALVTRPTDVAAAYDCAEANP
                     EWAALAAPTGPLYPWDPASTYLRPPPFATVDASTRLGRYTAHPLLVLGDDVTTDHISP
                     AGAIPADSEAGRWLVERGEDPTDLNVYAARRGNWEVMVRGLYTNPTVRNLLQPGLAPG
                     TTLHVPSGEVLPLWHVAGLYAQRGDALVVVAGERYGTGSSRDWAAKGPHLLGVRAVLA
                     CGFERIHRSNLCNMGILPLELPAGTSPATLQIAPGDRFEIDVPAATLAPSSACPVTVQ
                     RAHGAAQRFVAKAAVETLAEVEVLRAGGLLPLMLRRCLSLTGS"
     gene            1907..3220
                     /locus_tag="VD737_07655"
     CDS             1907..3220
                     /locus_tag="VD737_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015137 - citrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015746 - citrate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate:proton symporter"
                     /protein_id="HXW10472.1"
                     /translation="MLSLLAFGMVATFMTLIMTKRLSAMIALIVVPTVFAIIAGFASD
                     LGPMMLAGIQDLAPTGVMLLFAILYFGLMIDAGLFDPLTRKVVELAHGDPLRITMGSA
                     ILGLVVAFDGDGTTTYMITLSALLPLYRHMKMDVRVLTCILMMAVAAMNLVPWGGPTA
                     RAAIALGVDPRELFQSLIPPIIATMGWVLLVAFLLGRAERRRLGISRRIAMPPPGELD
                     LSTPDSDGTPSTRRPRLFWFNAALTATLMSVLLFGDVPLQVLFMVGFAIAAMVNYPGI
                     EMQRERLAAHAANALATAGLVFAAGIFTGVLSGTKMVDAMAGTVTGVLPDALGPYMAP
                     ITALLSIPFTVLISNDAFYFGMMPILAHSAELHGIDAMQIARASLVGQQIHLLSPLVA
                     STYLLVGTAGVELGDHQRFTMKWALGACGVFLATSCLLGLFPWRA"
     gene            complement(3226..4308)
                     /locus_tag="VD737_07660"
     CDS             complement(3226..4308)
                     /locus_tag="VD737_07660"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXW10473.1"
                     /translation="MSGPRWWLAWLLLAACEVATAAGTPRGYPRSYADVVEAARREGS
                     VVVYSTTDREEVRGALAAFRKRYPFLTVEYHELESPTLYERVVAESRARVATGDLVWS
                     SAMDLQIKLVNDGYAQAYASPEKPNLPDWAIWKNEAWGVTAEPVVIAYNKRLVPAADV
                     PRTHDAFERLLRAKTEAYVGRVSTYDPRRSGFGYLQLTQDIHVRRGDVWSLAEALGMV
                     RARQHSSSRSLLAEVESGRSALAYNALGSYALKRATRNPDIGVVMPEDYTLVVSRIAL
                     ITREARQPHAAKLLLDFLLSSEGQQELAAAYMAPSREDVTPLAGTKPPPARARPVRLA
                     PSLLANLDAIKRERLLARWQSALAGP"
     gene            4381..5064
                     /locus_tag="VD737_07665"
     CDS             4381..5064
                     /locus_tag="VD737_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXW10474.1"
                     /translation="MARILLVEDDAALVRGLAAILRGEGYSVDVAPDGSSALALVRDE
                     PFAVILLDVNLPGMSGFEVLRTLRARGCHAPILLLTARDALEDRVKGLDLGADDYMLK
                     PFEPPELAARVRALMRRPAADPSPVIHVGNLEIDRSRCTIRVAGEQVELRRREWVLLE
                     RLAVRVGKVVSKERLATEMFGFDEVVAPNAIEVYVARLRRKLGPHGPAIRTMRGLGYV
                     MEPGPHGAA"
     gene            5054..>5687
                     /locus_tag="VD737_07670"
     CDS             5054..>5687
                     /locus_tag="VD737_07670"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HXW10475.1"
                     /translation="MRHNLRVSLTGRLLVALILPTATLALMLGVGGALFIDRIVESVN
                     DRLLETSARSIADTLAFEHGDITLDLPPSAFGMLENDERDNVYYSVHVGRDLLTGYAD
                     LPRVETRRAPVDDTRFAYARYRGSPVRLAAVVRRVPRVESPVVVQVAETLDSRSALQR
                     RLRVGLFVLEVLLIVPLVVLVPLAVRWGLRPLAEVRQEVDSRSPVDFTPLK"
BASE COUNT          786 a         2010 c         1989 g          902 t
ORIGIN      
        1 ggggcgggcg tgacggcgac ggacctggcg ctgaacgtca cgcagcggct gcgcgaaatc
       61 gacgtcggcg gacgcttcgt ggagttctgc ggtccgggcg tggcggagat cacggcgggc
      121 acgcgcgcgg cggtcgcgaa catggcgccg gagttcggcg gcgcgaccgg ctacttcccg
      181 atcgacgagc gcacgctcga gtacctgcgc gcgaccggtc gtgacgacga ccacgtcgcc
      241 cgggtgggac gctacgcgcg gcagcagcag ctgtggttcg acccgcaggc gcgaccggac
      301 tacgaccggc tcgtggagtt cgatctcgat tccgtgcggc cgagcgtcgc gggcccgcgg
      361 cgaccgcagg accgcatcga tcgctcggcc gcgcccgccc tggccggcga cgatcgccag
      421 cctcggccga tcgcgatcgc cgccatcacg agctgcacca acacttcgga cgcgaggttg
      481 ctggtcgccg caggcctgtt ggcgcgcaac gcgatcgagc gcggcctgcg cgtgccgcgg
      541 tgggtcaaga ccagcctggc gcccgggtcg ccgaccgccc ggatcgtgct gcagagggcc
      601 ggcgtgctcg gcgcgctcga gagactcggg ttcgcgatcg tcgcccatgg ctgcaccacg
      661 tgcatcggca acagcgggcc cctgaccgcc gtcatggaaa ccatggtgga gtccggcgcg
      721 cggcctgtcg ccgtgctgtc gggcaaccgg aattttccgg gacgcgtgca ccgtcgtatc
      781 acggacgcct atctcgcgtc gccgccgctc gtcgtggctt acgcgctggc cggcgacttc
      841 gatcgtcgca tcgaagcgga accgctcggt cactcgccga ccggccaggc cgtttacctg
      901 cacgaactct ggcctccgga cgaagaggtc gacgccgtga tcgcgctcgt gacccgcccg
      961 accgacgtcg ccgcggccta cgactgcgcg gaagccaacc ccgagtgggc ggcgctcgcg
     1021 gcccccacgg gcccgctgta cccctgggat cccgcgtcga cctatctgcg accgccgccg
     1081 ttcgccacgg tcgacgcttc gacccgcctc ggtcgctaca cggcgcaccc gctgctggtg
     1141 ctgggagacg acgtcacgac ggaccacatc tcgccggccg gcgcgatccc ggcagacagt
     1201 gaggcgggac gctggctcgt cgaacgcggc gaggatccca ccgacctcaa cgtctacgcg
     1261 gcccgccgtg gcaactggga agtcatggtg cgcggcctgt ataccaatcc gaccgtgcgc
     1321 aacctgctgc agccgggcct cgccccgggc acgacgctgc acgtaccgtc cggcgaggtg
     1381 ctgccgttgt ggcacgtcgc cgggctgtat gcgcagcggg gcgacgcgct ggtcgtggtg
     1441 gccggggagc gctacgggac cggctcgtcg cgggactggg ccgccaaggg accgcacctg
     1501 ctcggggtgc gggccgtgct cgcctgcggc ttcgagcgca tccaccgctc caacctgtgc
     1561 aacatgggca tcctcccgct cgaactgccc gccggcacct cgccggccac gctgcagatc
     1621 gctccaggcg accggttcga gatcgacgtt cccgccgcca cgctcgcgcc ctcgtccgcc
     1681 tgccccgtca ccgtgcaacg cgcccacggc gccgcgcagc gattcgtcgc gaaagcggcc
     1741 gtcgagaccc tggcggaagt cgaggtgctg cgcgccggcg gcctgttgcc gctcatgctc
     1801 cgccgctgcc tgtcactgac aggttcgtga aagtcatcgc gcactacgat gcccaccagc
     1861 caggacgtcg gcggccgtgc cgccggcgcc ggaggtgcca cgcttgatgc tttccctcct
     1921 cgcgttcggc atggtcgcga cgttcatgac cctgatcatg acgaagcgcc tgtccgccat
     1981 gatcgcgctg atcgtcgtgc cgacggtgtt cgcgatcatc gcgggcttcg ccagcgacct
     2041 cggcccgatg atgctggccg gcatccagga cctcgccccc accggcgtga tgctgctgtt
     2101 tgcgatcctg tacttcggac tgatgatcga cgccggactc ttcgatccgc tgacgcgcaa
     2161 ggtggtcgaa ctcgcgcacg gcgacccgct ccggatcacg atgggttccg cgatcctcgg
     2221 gctcgtggtc gcgttcgacg gcgacggcac cacgacgtac atgatcacgc tctcggcgct
     2281 gctgccgctc taccggcaca tgaagatgga cgtgcgggtg ctcacctgca tcctgatgat
     2341 ggcggtggcc gcgatgaacc tcgtgccctg gggcggtccc accgcacgcg cggcaatcgc
     2401 gctgggcgtc gacccgcggg aactgttcca gtcgctgatt ccgccgatca tcgcgaccat
     2461 gggctgggtc ctgctggtcg ccttcctgct cggtcgtgcc gaacgtcgcc gactgggcat
     2521 ctcgcgacgc atcgccatgc cgccgcccgg cgagctggac ctgtcgaccc ccgacagtga
     2581 cggcactccg tcgacgcggc gtccgcgcct gttctggttc aacgccgcgc tcaccgcgac
     2641 gctgatgtcg gtgctgctgt tcggcgacgt tccgctgcag gtgctcttca tggtgggctt
     2701 cgcgatcgcc gccatggtca attaccccgg catcgagatg cagcgtgagc gcctggccgc
     2761 gcacgccgcc aacgcgctcg cgactgcggg gctcgtgttc gccgcgggga tcttcaccgg
     2821 cgtgctctcc ggcacaaaga tggtcgacgc gatggcgggc acggtgaccg gcgtgctgcc
     2881 tgacgcgctg ggaccctaca tggcgccgat caccgcgctg ctcagcatcc cgttcacggt
     2941 gctgatctcg aacgacgcgt tctacttcgg gatgatgccg atcctggcgc actcggccga
     3001 actgcacggc atcgacgcga tgcagatcgc ccgcgcgtcg ctggtcggcc agcagatcca
     3061 cctgctcagc ccgctcgtgg cgtcgacgta cctgctggtc ggcaccgcgg gcgtggaact
     3121 cggcgatcac cagcggttca cgatgaagtg ggcgctgggc gcgtgtggcg tgttcctcgc
     3181 gacgagctgc ctgctcgggc tctttccctg gcgggcttga caggcctagg ggccggccag
     3241 cgcggactgc cagcgcgcga gcaaacgctc gcgcttgatg gcgtcgaggt tcgcgagcag
     3301 cgacggggcc agccgtaccg gacgcgctct cgcgggcggc ggcttggtgc cggccagcgg
     3361 cgtcacgtcc tcccgcgagg gagccatgta ggccgcggcc agttcctgct gtccttcgga
     3421 cgacagcagg aagtcgagca gcagtttcgc ggcgtgaggc tgccgggctt cacgggtgat
     3481 caacgcgatc cgcgagacga ccagcgtgta gtcctcgggc atgaccacgc cgatgtcggg
     3541 attgcgcgtg gcacgcttga gcgcgtacga accgagcgcg ttgtaggcga gcgcgctgcg
     3601 gccgctctcg acctcggcga gcagcgagcg gctggacgag tgttgtcgcg cgcgcaccat
     3661 gccgagcgcc tccgccaggg accagacgtc gccgcggcgc acgtggatgt cctgcgtgag
     3721 ctgcaggtaa ccgaaaccgg agcgccgtgg gtcgtacgtg gagacgcgac cgacgtaagc
     3781 ctcggtcttc gctcgcagca ggcgctcgaa cgcatcgtgg gtgcgcggga cgtcggccgc
     3841 cggcacgagg cgcttgttgt aggcgatcac cacgggttcg gccgtcactc cccaggcctc
     3901 gttcttccag atcgcccagt ccggcaggtt cggcttctcg ggcgaggcgt acgcctgcgc
     3961 gtagccgtcg ttcacgagct tgatctgcag gtccatcgcc gaactccaga cgaggtcgcc
     4021 cgtcgcgacg cgtgcgcgcg actcggcgac gacgcgctcg taaagcgtcg gggactcgag
     4081 ttcgtggtat tcgaccgtga ggaacggata gcgcttgcgg aacgcggcca gggcaccgcg
     4141 gacctcctcg cggtcggtcg tcgaataaac caccacggag ccctcgcgtc gcgccgcctc
     4201 gacgacgtcg gcgtacgagc gtggatagcc gcgcggcgtg ccggccgccg tggcgacctc
     4261 gcacgcggcc aggagcagcc aagccagcca ccagcgaggc ccggacatcg gtcttttgcc
     4321 tgcgatcccg gtcatctcca tactatggaa atcgatcgtc accgttgtca aagagagggc
     4381 atggcaagga tcctgctggt cgaggacgac gcggcgctgg tacgcgggct cgcggcgatc
     4441 ctgcgcggcg aaggttactc ggtcgacgtc gcgcccgacg gatcgagcgc gcttgcgctc
     4501 gtgcgcgacg agccgttcgc ggtgatcctg ctggacgtga acctgccggg gatgagcggc
     4561 ttcgaggtgc tgcgcacgct gcgagcgcgc ggctgccacg cgccgatcct gctgctgacg
     4621 gcgcgcgacg ccctcgagga ccgggtcaag ggcctggacc tcggcgccga cgactacatg
     4681 ctgaagccct tcgagccacc ggagctcgcg gcgcgcgtac gcgcgctgat gcgccggccc
     4741 gcggccgatc cctcgcccgt catccacgtc ggcaacctcg agatcgaccg ttcacgctgc
     4801 accatccggg tggcggggga acaggtcgag ctgcgccggc gcgaatgggt gttgctggag
     4861 cgcctcgccg tgagggtcgg caaggtcgtc tcgaaggaac ggctcgcgac cgagatgttc
     4921 ggcttcgacg aggtggtcgc gcccaacgcg atcgaggtct acgtggcccg gctgcggcgc
     4981 aagctcgggc cgcacggacc cgccatacgc acgatgcgcg gcctcggcta cgtgatggag
     5041 cccggcccgc acggtgcggc ataacctgcg cgtctcgctg accggccgcc tgctggtcgc
     5101 actgatcctg ccgacagcca cgctggcact gatgctcggc gtcggcggcg ccctcttcat
     5161 cgaccggatc gtcgaaagcg tcaacgaccg cctgctcgag acctccgccc gctcgatcgc
     5221 cgacacgctc gcgttcgagc atggcgacat tacgctcgac ctgccgccct ccgcgttcgg
     5281 catgctcgag aacgacgaac gcgacaacgt gtactacagc gtgcacgtcg ggcgtgacct
     5341 gctgacgggg tacgcggacc tgccgcgcgt ggagacgcgc cgtgcgcccg tcgacgacac
     5401 gcgtttcgcg tacgcgcgct atcgcggctc gccggtgcgc ctcgcagccg tggtgcggag
     5461 ggtgccgcgc gtggagtcgc ccgtggtcgt ccaggtggcc gagacgctgg actcgcgcag
     5521 cgcgctgcag cgacgcctgc gcgtgggcct gttcgtgctg gaggtgctgc tgatcgtgcc
     5581 gctggtggtg ctggtgccac tggcggtgcg ctggggcctg cggccactgg cggaggtgcg
     5641 gcaggaagtc gacagccggt cgcccgtcga cttcactccc ttgaagc
//
LOCUS       DASPFR010000289         3715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1304070, whole genome shotgun sequence.
ACCESSION   DASPFR010000289 DASPFR010000000
VERSION     DASPFR010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304070"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1924
                     /locus_tag="VD737_07675"
     CDS             173..1924
                     /locus_tag="VD737_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HXW10476.1"
                     /translation="MNGTNGGEVLARMLLAEGVEKVFGIIDGTYFGFYSNLHKLGIEI
                     VTPRHETCAAHMAGAYARLTGRLGVCMASNGPGVANLLPGLVVENAEGNRVLAITSCR
                     RPQIVYPDRGGAYQAFDQVGAIKHFAKWSNAVVSFDRVAELGRTALRHCYEGRPGVVH
                     LDVPETIMNGKVKVEVPTWQPGQFRRSHALVPAPDQVEEAAELLVAADQAIIHAGSGV
                     IHAGAYDELRRVAELLCAPVTTSWAARGVLPETHELAIPMPLIKLNHQVRNDADVVLV
                     LGSRLGETDWWGKAPYWRRPSEQKMVQVDVDGHVLGASRPATVAALADVRLFLVALAD
                     AIERRGGRANLDARRRRLVQYRETIRAERAKLDEKLQDLERPMNPAHVAHVCQRVFPE
                     GTTLVADGGNTAIWAMMFHQMRVPNTLLSTFKFGMLGAGVAQALGAAVARPDRPVCCI
                     IGDGAMGFHPQEIETAVRNRLRVVYVVVCDRQWGMVKMNQQFALKPVKTLLTKALGPD
                     ETIKADLGEIRFDKVAEAMGAHGERVADPGELEAALVRALASGKPAVVHVDVDPVKHM
                     WAPGLMHFKDMHAEPKG"
     gene            1934..2860
                     /locus_tag="VD737_07680"
     CDS             1934..2860
                     /locus_tag="VD737_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HXW10477.1"
                     /translation="MNELDAVRLNFSPESLVVLDLILAFVLFGVALDMRVQDFKGLLT
                     SPRGTLIGLLAHSVLLPAVAWALTMALRPSPSIALGMILVASCPSGNISTFLVNYARG
                     NTALSVTIAAFSMLGSIAFTPFNLTFWGTLNPDTRSILHAVTLSPWEVFGTIVVLLGI
                     PTVLGMWVANRWPRFAERARRPFRTLSLLFFVAFVAAALAANWDFFLDYVGRVVLFVF
                     LLNAFALLTGYFTARAFRLAEPDRRAVAFEVGIQNSGFGLVLVFNFFGGLGGMAIVAA
                     WWGIWHILVGTTLATIWRRFPPRQAVRAEVPA"
     gene            2857..>3715
                     /locus_tag="VD737_07685"
     CDS             2857..>3715
                     /locus_tag="VD737_07685"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXW10478.1"
                     /translation="MSGRRIFITGGAGYLGSALVETLAPRLAAGEFEVVVASDVREVP
                     ADRRRAGVEYVVHDVCTPGLDAPLASRQIDTVVHLAAIVTPGAQSNRELEYAVDVLGT
                     RHVLEACVAAGVGQLIVTSSGAAYGYHADNPEWLDEDDAVRGNEAFAYSYHKRLVEEM
                     LAEYRQRHPALRQVVFRVGTILGDTTRNQITALFDKPRLIAIRGSRSPFVFVWDRDVV
                     GAIEHAIRTDAAGIYNVAGDGALSIQDIARRLGKRCVTLPPGLLRTVLAVLHPLGLVQ
                     YGPEQVDFLR"
BASE COUNT          546 a         1258 c         1317 g          594 t
ORIGIN      
        1 gtggtcagtg gtcagtggct agtgaagagg aggagcatca gggccacgag gtcttcggag
       61 ttgcgcacta gccacaagca actagccact agccactagc cactagccac ttcctccccg
      121 gtatattcgg ccgaaaaccg acgacggcac gtcgacgacc gggggtttcc acatgaacgg
      181 gacgaatggc ggcgaggtcc tcgcgcgcat gctgctggcc gagggtgtcg aaaaggtctt
      241 cggcatcatc gacggcacct acttcgggtt ctattcgaac ctgcacaagc tcggcatcga
      301 gatcgtcacg ccgcggcacg agacctgcgc tgcgcacatg gccggcgcgt acgcgcggct
      361 cacgggcagg ctcggcgtct gcatggcgag caacgggccg ggcgtcgcaa acctgttgcc
      421 gggcctggtc gtcgagaacg ccgaaggcaa ccgcgtgctc gccatcacga gctgccgccg
      481 gccgcagatc gtctaccccg accgcggcgg cgcctaccag gcgttcgacc aggtcggcgc
      541 gatcaagcac ttcgccaagt ggagcaacgc cgtcgtctcg ttcgaccggg tcgcggaact
      601 cggccggacg gcgctgcgtc attgctacga aggccggccc ggtgtcgtac acctcgacgt
      661 gccggagacg atcatgaacg gcaaggtcaa ggtcgaggtg ccgacctggc agccgggcca
      721 gttccgccgc tcgcacgcgc tcgtccccgc gccggaccag gtcgaagagg ccgccgagct
      781 gctggtggcg gccgatcagg cgatcatcca cgccggcagc ggcgtgatcc acgccggcgc
      841 ctacgacgag ctgcgtcgcg tggcggagct gctctgcgcg ccggtgacga ccagctgggc
      901 ggcgcgcggc gtgctgccgg agacgcacga gctcgcgatc ccgatgccgc tgatcaagct
      961 caaccaccag gtgcgcaacg acgccgacgt cgtgctggtc ctgggttcgc ggctcggcga
     1021 gaccgactgg tggggcaagg cgccgtactg gcggcgaccg tccgagcaga agatggtgca
     1081 ggtcgacgtc gacggccacg tgctcggcgc gagccgaccc gccaccgtcg ccgcgctcgc
     1141 cgacgtgcgg ctgttcctgg tcgcgctcgc cgacgccatc gagcgtcgtg gcgggcgtgc
     1201 caacctcgac gcgcgccggc gtcgcctcgt gcagtaccgc gagacgatcc gcgccgagcg
     1261 cgcgaagctc gacgagaagc tgcaggacct cgagcggccg atgaatcccg cgcacgtggc
     1321 gcacgtgtgc cagcgcgtgt tcccggaggg cacgacgctg gtcgccgacg gcggcaacac
     1381 ggcgatctgg gccatgatgt tccaccagat gcgcgtcccg aacacgctgc tctcgacgtt
     1441 caagttcggc atgctcggcg ccggcgtcgc ccaggcgctc ggcgccgcag tggcgcggcc
     1501 cgaccggccg gtctgctgca tcatcggcga cggcgcgatg ggcttccatc cgcaggaaat
     1561 cgagacggcg gtgcgcaacc ggctccgcgt cgtatacgtg gtcgtctgcg accggcagtg
     1621 gggcatggtc aagatgaacc agcagttcgc gctgaagccg gtcaagacgc tgctcaccaa
     1681 ggcgctcggt cccgacgaga ccatcaaggc cgacctgggc gagatccgct tcgacaaggt
     1741 cgccgaggcg atgggtgcgc acggcgagcg cgtggccgat ccaggcgagc tcgaggcggc
     1801 cctcgtgcgc gcgctcgcaa gcggcaagcc ggcggtcgtg cacgtggacg tcgacccggt
     1861 caagcacatg tgggcgccgg gactcatgca cttcaaggac atgcacgccg agccgaaagg
     1921 ctgaggcgcg cgcgtgaacg aactcgacgc ggtccggctg aatttcagcc cggagtcgct
     1981 cgtcgtcctc gacctgatcc tcgcgttcgt gctgttcggc gtggcgctgg acatgcgcgt
     2041 gcaggacttc aagggcctcc tgacgtcgcc gcgcggcacg ctgatcgggc tgctcgccca
     2101 ctcggtgctg ctgccggccg tggcctgggc gctcacgatg gcgctgcggc cgagcccgag
     2161 catcgcgctc ggcatgatcc tcgtggcgtc gtgcccgagc ggcaacatct cgacgttcct
     2221 ggtcaattac gcgcgcggca acacggcgct gtcggtgacg atcgcggcgt tctcgatgct
     2281 gggatcgatc gcgttcacgc cgttcaacct gacgttttgg ggaacgctca accccgacac
     2341 gcgctcgatc ctgcacgcag tcacgctgag cccgtgggag gtgttcggca cgatcgtcgt
     2401 gctgctcggc atcccgacgg tgctcggaat gtgggtggca aatcgctggc ctcgattcgc
     2461 agagcgtgcg cgccggccgt tccgcacgct gtcgctgctg ttcttcgtcg cgttcgtcgc
     2521 cgcggcgctg gcggccaact gggacttctt cctggactac gtggggcgcg tcgtgctgtt
     2581 cgtgttcctg ttgaacgcgt tcgcgctgct gaccggttat ttcacggcgc gcgcgttccg
     2641 gctggccgag cccgaccggc gcgccgtcgc attcgaggtc ggcatccaga actccggttt
     2701 cggcctcgtg ctggtcttca acttcttcgg cgggctcggc ggcatggcga tcgtggccgc
     2761 ctggtggggc atctggcaca tcctggtcgg cacgacgctg gcgacgatct ggcgtcgttt
     2821 tccgccgcgc caggcggtgc gggccgaggt gccggcgtga gcggccggcg gatcttcatc
     2881 accggcggcg ccggctatct cggcagcgcg ctggtcgaga cgctcgcgcc acgactggcg
     2941 gcgggtgagt tcgaggtcgt ggtcgcgagt gacgtgcggg aggtgccggc cgaccggcgt
     3001 cgcgccggcg tcgagtacgt cgtgcacgac gtgtgcaccc ccggcctcga cgcaccgctc
     3061 gcgagccggc agatcgacac cgtcgtgcac ctcgccgcaa tcgtgacgcc gggcgcgcaa
     3121 tcgaatcgcg aactcgaata cgcggtggac gtgctgggca cgcgccacgt gctcgaggcc
     3181 tgcgtggcgg cgggcgttgg ccagctgatc gtgacgtcga gcggcgccgc gtacggctat
     3241 cacgcggaca accccgagtg gctcgacgag gacgacgcgg tgcgcggcaa cgaggcgttc
     3301 gcgtactcgt accacaagcg gctcgtcgag gagatgctgg cggagtaccg gcaacggcac
     3361 cccgcgctgc ggcaggtggt atttcgcgtc ggcacgatcc tcggcgatac gacgcgcaac
     3421 cagatcacgg cgctgttcga caagccgcgg ttgatcgcga tccgcggttc gcgcagcccg
     3481 ttcgtgttcg tctgggaccg cgacgtcgtg ggcgcgatcg agcacgcgat ccgcaccgat
     3541 gcggccggca tctacaacgt cgcgggcgac ggcgcgctct cgatccagga catcgcgcgg
     3601 cggctcggca agcggtgcgt gacgctgcct cccggcctgc tgcgcaccgt gctggcggtg
     3661 ctgcatccgc tcgggctcgt gcagtacggc cccgagcagg ttgatttcct gcgct
//
LOCUS       DASPFR010000290         3964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1791284, whole genome shotgun sequence.
ACCESSION   DASPFR010000290 DASPFR010000000
VERSION     DASPFR010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791284"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1806)
                     /locus_tag="VD737_07690"
     CDS             complement(<1..1806)
                     /locus_tag="VD737_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10479.1"
                     /translation="MNARAGGKRLWRLLAGSLAALLVVAALLMTGLRIAIAYLPEHAD
                     NLRSWVERETRMRIEYDGLDARLRAYGPELVLTGVRVLDEQQGQTLFTSREGRIGLDL
                     WNFFRTGQLVAGRVHLEGPRVTIVRLPDGRIRLLGLAERPADRPPFDFDRLPAGRVVI
                     EDATVVFRDQLANQPPLELRELEGELRRDHDAVRIEGRAELPRALGSTAGFEVQLKGS
                     LDDRAHLDARAEIDVDDLRVAGVAPFVPGRYARPLAGGGPVRVVLAVRQGQVVQARLD
                     LDLRDAAFGVPPRRVPTVETVRVTEPRLELADGSFMRHATVTKEVEQRPARPLPAEVR
                     FATLSGQVRLRRDGASWVLRADDLRVQPRDMPASQPARLEGRWWGHPESRFGLRLAAE
                     HLDLQRVWPLGLAFAPPAFDRWTGLAPTGRISSIRLEATRERAGLAPAFTVDADVAAF
                     GVLAQGRLPGVTGLTAKVSGTDQRGTLELRTGEAAFDWPRQFRAPIRVQDAVADVQWR
                     REGDAWHVATQGARLRHDQARATLDASLQWVKPSISPVLDLDATVEGLDVTSVPLFIP
                     VGRLRERTIAWLDRAFVAGRASNGRVRYHGPVRQFP"
     gene            complement(1803..3272)
                     /gene="rng"
                     /locus_tag="VD737_07695"
     CDS             complement(1803..3272)
                     /gene="rng"
                     /locus_tag="VD737_07695"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HXW10480.1"
                     /translation="MSTEMIVNVSPRETRAALLENGVLQELFVERASRMGLTGNLYKG
                     RVSRVLPGMQAAFIDIGLERTAFLHVSDIVQPADAEDSAEAPRTENIRELVNEGGDIL
                     VQVLKDPLGTKGARLTTFITIPSRYLVYMPFGHGVGISARIEAEEERSRLRELMQSFV
                     TPGESGGYIVRTAAEAAAGEALRADMLFLRKLWEVLSGKAAGARAGTLVHEDLPLPLR
                     LMRDLVGEGVDRVLVDHERTCERMQEFATTFMPALGARIERYTGSRPIFDLHGVEEEI
                     QRSLDRKVPLKSGGYLIIDQTEALTTIDVNTGAYVGHRNLEETIYRTNLEAAVAIARQ
                     LRLRNLGGIIIIDFIDMEEEEHRRQVLQALEKALAKDHAKTHVSAVSPLGLVEMTRKR
                     TRESLAHQLCAPCPACEGRGFVKTPETVCYEIFREVLRQAGQFDFQQLMVLAHSDVIE
                     RLLDEESAALGELEVLVGKPIRLQTEALYLHDQYDVVLM"
     gene            complement(3269..3853)
                     /locus_tag="VD737_07700"
     CDS             complement(3269..3853)
                     /locus_tag="VD737_07700"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HXW10481.1"
                     /translation="MIYLASASPRRSALLQQVDVPHEVRPVDVDETPRPGEPPARYVR
                     RLAEEKARALWATLRPADRKPVLAADTTVALGDEILGKPVDRDDAARILGRLSGRDHE
                     VHTAVALLHDGGAAARVSTSTVSFRELTPAEIAWYWSTGEPADKAGAYAVQGQGAIFV
                     RHLAGSYSGVMGLPLYETWELLAPVLGLTGRGAA"
BASE COUNT          564 a         1419 c         1399 g          582 t
ORIGIN      
        1 tgggaactgg cgcacgggac cgtggtaccg gacgcgacca ttgctcgcgc gaccggccac
       61 gaatgcgcgg tcgagccacg cgatggtgcg ctcgcgaagc cgcccgaccg gaatgaacag
      121 cgggaccgag gtgacgtcga ggccctcgac ggtggcgtcg aggtcgagca ccggcgaaat
      181 cgacggtttg acccattgca gcgaggcgtc gagcgtcgca cgtgcctggt cgtgccgaag
      241 cctcgcgccc tgcgtggcga cgtgccacgc gtcgccctcc cggcgccatt gcacgtcggc
      301 gaccgcgtcc tgcacgcgga tcggcgcacg aaattgccgc ggccagtcga aggcggcctc
      361 gccagtgcgc agctcgagcg tgccgcgctg gtcggtaccg gagaccttcg cggtcaaccc
      421 cgtgacgcca ggcaggcggc cctgcgccag gacgccgaag gcagcgacgt cggcgtcgac
      481 ggtgaacgcc ggtgcgagcc cggcgcgctc gcgcgtcgcc tcgagacgga tgctcgaaat
      541 gcgtccggtc ggcgcgaggc ccgtccagcg gtcgaacgcc ggcggcgcga acgcgagccc
      601 gagcggccac accctctgca ggtcgaggtg ctccgcggcg agcctgagcc cgaaccgcga
      661 ctcggggtgg ccccaccagc ggccttcgag tcgggcgggt tgcgacgccg gcatgtcccg
      721 cggctggacg cgcaggtcgt cggcacgaag tacccagctc gcgccgtcac gccgcaggcg
      781 aacctggcca ctcaacgtcg caaagcgcac ctccgcgggc agaggccgcg ccgggcgctg
      841 ctcgacctcc ttggtgacgg ttgcgtgacg catgaagctg ccgtcggcaa gttcgagccg
      901 cggctctgtc acgcgcacgg tctcgacggt gggcaccctg cgtggcggca cgccgaacgc
      961 cgcgtcgcgc aggtcgagat cgagccgcgc ctgcacgacc tggccctggc gcacggccag
     1021 caccacgcgg accggcccgc ccccggcgag cggccgtgcg tatcgaccag gcacgaacgg
     1081 tgcgacgccg gcgacgcgca ggtcgtcgac gtcgatttcc gcgcgtgcgt cgaggtgcgc
     1141 gcggtcgtcc agcgatccct tcaactgcac ttcgaatccg gcggtcgacc cgagcgcgcg
     1201 cggcagctcc gcgcgacctt cgatgcggac cgcgtcgtgg tcacggcgca gctctccctc
     1261 gagttcgcgc agctcgagcg gaggctggtt cgcgagctgg tcgcggaaga cgaccgtcgc
     1321 gtcctcgatc acgacgcgac cggcgggcag ccggtcgaag tcgaacggcg gacggtccgc
     1381 cggtcgttcg gcaaggccga gcagccggat ccggccgtcg ggcagccgca cgatcgtcac
     1441 gcgcggcccc tcgaggtgca cgcggccggc gaccagctgc cccgtgcgga agaagttcca
     1501 caggtcgaga ccgatgcggc cttcgcgcga cgtgaacagc gtctgcccct gttgctcgtc
     1561 gagcacgcgc acgccggtca gcacgagctc gggaccgtag gcgcgcaacc gcgcgtcgag
     1621 cccgtcgtac tcgatgcgca tgcgcgtctc gcgctcgacc cagctgcgca ggttgtcggc
     1681 gtgctccggg aggtaagcga tcgcgatgcg cagcccggtc atcagcaacg ccgcgacgac
     1741 gagcagcgcg gcgagcgagc ccgcgagcag gcgccagagg cgcttgccgc ccgcccgtgc
     1801 gttcacatga gcaccacgtc gtactggtcg tgcaagtaca gcgcctcggt ctgcagccgg
     1861 atcggcttgc caaccagcac ttcgagctct cccagcgccg cggattcctc gtcgagcagc
     1921 cgctcgatca cgtccgagtg cgcgagcacc atcagctgct ggaagtcgaa ctggccggcc
     1981 tgccgcagca cctcgcggaa gatctcgtag cagacggtct cgggcgtctt gacgaagccg
     2041 cgcccttcgc aggccgggca cggtgcgcac agctggtgcg cgaggctctc gcgcgtgcgc
     2101 ttgcgggtca tctcgacgag cccgagcggc gacaccgccg agacgtgcgt cttggcgtgg
     2161 tccttggcga gcgccttctc gagcgcctgg agcacctggc ggcggtgctc ctcttcttcc
     2221 atgtcgatga agtcgatgat gatgatgccg ccgaggttgc gcaggcgcag ctggcgcgcg
     2281 atcgccacgg ccgcctcgag gttggtgcgg tagatcgtct cctcgaggtt gcggtggccc
     2341 acgtatgcgc cggtgttgac gtcgatcgtc gtgagcgcct cggtctggtc gatgatcagg
     2401 tagccgcccg acttgagcgg caccttgcgg tccagcgagc gctggatctc ctcctcgacg
     2461 ccgtgcaggt cgaagatcgg gcgactgccc gtgtagcgct cgatgcgcgc gccgagcgcc
     2521 ggcatgaacg tggtcgcgaa ctcctgcatg cgctcgcagg tgcgctcgtg atcgacgagc
     2581 acgcggtcga cgccctcgcc gacgaggtcg cgcatcaggc gcagcggcaa cggcaggtcc
     2641 tcgtgcacga gtgtgccggc cctggcgcct gcggccttgc ccgacagcac ttcccacagt
     2701 ttgcgcagga acagcatgtc ggcccgcagc gcctctccgg ccgccgcctc ggccgcggtg
     2761 cgcacgatgt agccgcccga ctcgccaggc gtcacgaacg actgcatcag ttcgcgcagc
     2821 cggctgcgct cttcctcggc ctcgatgcgc gcggagatgc ccacgccgtg cccgaacggc
     2881 atgtagacga ggtagcgcga cgggatcgtg atgaacgtgg tcaggcgggc acccttggtc
     2941 ccgagcggat ccttgagtac ctgaacgagg atgtccccgc cttcgttgac cagctcgcga
     3001 atgttttccg tgcgcggcgc ttcggcggaa tcctcggcat cggccggctg gacgatgtcc
     3061 gagacgtgca ggaaggccgt gcgctcgagc ccgatgtcga tgaaggccgc ctgcatgccc
     3121 ggcagcacgc gcgagacccg gcccttgtac aggttgcccg tcagccccat gcggctggcg
     3181 cgctcgacaa acagctcctg caggacgccg ttctcgagga gcgccgcgcg cgtctcgcgc
     3241 gggctgacgt tgacgatcat ctcggtgctc atgcggcgcc ccgaccggtc agaccgagca
     3301 ccggggcgag cagttcccac gtctcgtaca ggggcaggcc catgacgccg gagtagctgc
     3361 cggccaggtg gcgcacgaag atcgcgccct ggccctgcac cgcgtacgcg cccgccttgt
     3421 cggccggttc gccggtgctc cagtaccagg cgatctcggc cggtgtcagt tcacggaacg
     3481 acacggtgct ggtgctcacg cgtgccgcgg cgccgccgtc gtgcagcaac gccacggcgg
     3541 tgtgcacttc gtggtcccgg cccgacaagc ggccgaggat ccgcgccgcg tcgtcgcggt
     3601 cgaccggctt gccgaggatc tcgtcgccga gcgcgacggt cgtgtcggcc gcgagcacgg
     3661 gcttgcggtc cgccggccgc agggtcgccc agagcgcgcg cgccttttct tccgccaggc
     3721 gccgcacata gcgcgccggc ggctcgccgg gacgcggtgt ctcgtccacg tcgacgggtc
     3781 gcacctcgtg cggcacgtcg acctgctgca gcagtgcact gcgcctcggt gacgcggaag
     3841 ccaggtagat catcggcccg tcgcgctcgc gtcagccgtc gacgacggtc ggctcgagcc
     3901 gacgctcggc cagcagcgag ttgctgatca ggcagacctg ctccgccttc tccatgagct
     3961 tctc
//
LOCUS       DASPFR010000291         6283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1547709, whole genome shotgun sequence.
ACCESSION   DASPFR010000291 DASPFR010000000
VERSION     DASPFR010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547709"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VD737_07705"
     CDS             complement(<1..491)
                     /locus_tag="VD737_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXW10482.1"
                     /translation="MNHSPVGYRLARDVPLRTTFRVAARAEWFATVHDAGSLPELLDE
                     PDVRGLPVLVLGEGSNVLFVDPVFAGLVVHLACDDVRVLSGSTGDVAHVHVEAARNWD
                     ALVDWTLLRGMRGLENLALIPGTVGAAPVQNIGAYGLELKDRIESVEVVDLVTGRAAM
                     LPAS"
     gene            587..1978
                     /locus_tag="VD737_07710"
     CDS             587..1978
                     /locus_tag="VD737_07710"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HXW10483.1"
                     /translation="MNDSSTPRGVGTLAEAEAFLARHPDVETVQLAITDANGVGRGKN
                     LAREELLPFFGQGRNVAGSILGLDVTGEDVEGTGLVWEVGDADQCCRPIAGSLTVAPW
                     LSRPTAQVLGTMYEFDGRPAAADPRHALAHVVERCQERGHTPVLAVELEFYLLERDAS
                     GRPVPSSGLVSGVRRGRIDAYGLGRLDDMAPLFDDLYATASTLQLPVRTLMSEYAPGQ
                     FEITLEHRADALRAVDEAILFKRAVRGVAARHGRIACFMAKPFAELAGSGMHVHLSFD
                     DRARRNAFAAEDPAGSPLLREAIGGLQATLADGMAVFAPNANSYRRFRSQSYAPVSIN
                     WGINNRSVSLRIPAGPRSSRHVEHRVAGADANPYLVAALVLGGALHGLERQLDPGPPV
                     SGNGYAQGASSSRELPTQWHAALERAERSSFLRETLGAGFLDAYVAIKRQECEKFGAL
                     VSDHDYEWFLDTV"
     gene            2199..2816
                     /locus_tag="VD737_07715"
     CDS             2199..2816
                     /locus_tag="VD737_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXW10484.1"
                     /translation="MTIHLRKMYVDCRFGQLHMHTAFPSSGGFDELTPLVCLSPPSQT
                     GRLYRPLLRDLGRDRSVYAPDLPGAGESDAPDHPPAVADFAGACCDLLDSLRLKHVDV
                     IAYQAGSLAAIEFALARPQQVRRVALIGVPSSDAGYQVGERLPLLRQPVMVMRSRDEY
                     WESTGRTEPLLREARRVELQHPSGAVFEAGAEEVIRVARDFFDRA"
     gene            complement(2919..3344)
                     /locus_tag="VD737_07720"
     CDS             complement(2919..3344)
                     /locus_tag="VD737_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10485.1"
                     /translation="MRIELYMMMLAGIAGGLVGTLWSGLVSAPLFARWPALRPAAWQP
                     DTAARLLAGAVLYGLCGAAAGLLFWLSWGLVAVVSTPWHVIGAIYGGLLFAAGALPTL
                     GTLAIKARHHRGAVAVLAAEGLVAAVSAGVMCAYVWERQ"
     gene            complement(3374..4684)
                     /locus_tag="VD737_07725"
     CDS             complement(3374..4684)
                     /locus_tag="VD737_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXW10486.1"
                     /translation="MAPPISRRTLLGVLGTAAALGPVANAQTYTPQGVPPIDRYRWAW
                     ARAQLVLEPGLTWLDTASFGPTLRAVLVRTYRQLEEQSLDFRSFEALNGAGSAGERSV
                     LAAAASFLGCDAGELVLTDGARTGLALVAAGLDLQRDDEVLVSMHDHAAAVYPWIAQA
                     RRRGIRVVEAPATAASTATPEAIVQQFERSLTPRTRVACLAHIQDCDGTVLPIRELCA
                     VARSRGVFTVVDGALASGHVDFRLADLGCDAYATGVDRWLNGPLDAGLLYVRRDAQAR
                     VWPALPDRADGWNGTDRFNAALPVPPFEYAAATRFGNARVRRGASVAAIPLAFEFQDA
                     ISRPLVYGRIRELGSQLRSGLQRIAGVDVVTPPHFSLNAGILSVRLPGRDPGEIVERI
                     AREDRIVLGRVTHGAGFDAIRISVHPTNDAIDVDRAVAAVQRHV"
     gene            4610..6247
                     /gene="ilvA"
                     /locus_tag="VD737_07730"
     CDS             4610..6247
                     /gene="ilvA"
                     /locus_tag="VD737_07730"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HXW10487.1"
                     /translation="MRDRTQRGRGAEHAQQRAPGNRGSHDGKDVNVRRVGPDNAMQES
                     YIERILKARVYDVAIESPLEPARRLSRRLGNQVFFKREDLQPVFSFKLRGAYNKISTL
                     DEATARRGVICASAGNHAQGVALAARRRGIGAVIVMPRTTPGIKVQAVLDLGGDVVLH
                     GDDFDHAYEHALGLAAERGLTFVHPFDDPDVIAGQGTVGMEILRQCPTRPDAVFVPVG
                     GGGLIAGIAAYVKSLYPRVRIVGVEPEDAASMHDSLAQGERVTLDRVGIFADGVAVRR
                     VGEETFRLARQYVDEVVLVGTDEICAAIQDIFEDNRTIAEPAGALAVAGIKRYVAREN
                     CREQTLVAVNGGANINFDRLRHVAERADIGAQREALLAVEIPEEPGSFLRFCRLLGRR
                     SVTEFNYRYADARSARIFAGVALAEGRREKEALLELVAEAGYRVLDMSDDEMAKLHVR
                     YMVGGHARGLAHERLFRFEFPERPGALLKFLESIGVNWNISLFHYRNHGSDYGRVLAG
                     VQVPPADDAEFRRHLAELQYAYTDETLNPAYRMFLGD"
BASE COUNT          966 a         2215 c         2208 g          894 t
ORIGIN      
        1 gaagccggca gcatcgccgc gcggccggtc accaggtcga cgacctcgac gctctcgatc
       61 cggtccttca gctccagacc ataggcgccg atgttctgca ccggtgcggc acccacggtg
      121 ccggggatca gcgccaggtt ctcgaggccg cgcatgccac gcagcagcgt ccagtccacg
      181 agcgcgtccc agttgcgcgc ggcctcgacg tgcacgtgcg cgacgtcgcc agtcgaaccg
      241 gaaagcacgc ggacgtcgtc gcaggccagg tgcaccacga ggcccgcgaa gaccgggtcg
      301 acgaacagca cgttgctgcc ctcgccgagc accagcacgg gcagtccgcg cacgtccggc
      361 tcgtcgagca actcgggcag cgagccggcg tcgtgcaccg tcgcgaacca ctccgcgcgc
      421 gcagcgacgc gaaaggtcgt gcgcaacggc acgtcgcggg caagcctgta gccgaccggc
      481 gagtggttca tgcgtctcct ggggtgggaa tcccggcagc ttgccactgc tcgcgcggtt
      541 ccgtcactat cgcccggcac ggccagacga cgcgatcgcc cacagcatga acgattcgag
      601 cacaccgcga ggcgtcggca cgctggccga ggcggaagcc ttcctggcgc ggcatccgga
      661 cgtcgagacc gtgcagctcg cgatcacgga cgcgaacggc gtcggtcgcg gcaagaacct
      721 ggcccgcgag gaactcctgc cgttcttcgg ccagggacgc aacgtggccg gctcgatcct
      781 cgggctcgac gtcaccggag aggacgtgga gggcaccggc ctcgtctggg aggtcggcga
      841 cgcggaccag tgctgccggc cgatcgcagg cagcctcacg gtcgcgccgt ggctgtcccg
      901 gccgacggcg caggtgctcg gcacgatgta cgagttcgac ggccgtccgg cggcggccga
      961 cccgcgccac gcgctggcgc acgtcgtcga gcgatgccag gagcgcggac acacgcccgt
     1021 gctcgcggtc gaactcgagt tctatctgct cgagcgcgac gcgtccggac ggccggtgcc
     1081 gtcgagcgga ctcgtcagcg gcgtccgccg tggacgcatc gacgcgtatg gcctcggccg
     1141 cctggacgac atggcaccgc tcttcgacga cctgtacgcg actgcgtcga cgttgcagct
     1201 gccggtgcgc acgctcatgt cggagtacgc gccggggcag ttcgagatca cgctcgagca
     1261 ccgcgccgac gccttgcgtg cggtcgacga ggccatcctg ttcaagcggg ccgtgcgcgg
     1321 cgtggcggcc cgccacggcc ggatcgcctg cttcatggcc aagccgttcg cggagctggc
     1381 cggttccggc atgcacgtgc acctgagctt cgacgaccgc gcacggcgca acgcgttcgc
     1441 cgccgaggat cccgccggca gtccgctgct gcgcgaggcc atcggcggcc tgcaggcgac
     1501 gctcgccgac ggcatggccg tcttcgcacc gaatgccaac tcgtatcgcc ggttcaggtc
     1561 gcagagctac gcgccggtct ccatcaactg gggcatcaac aatcgctcgg tgagcctgcg
     1621 catcccggcc gggccgcggt cgagccggca cgtcgagcac cgcgtcgcag gggccgatgc
     1681 gaatccatac ctggtggccg cgctcgtgct gggcggtgcg ctgcacggcc tcgagcggca
     1741 gctcgacccg ggaccgccgg tctcgggcaa cggctatgcg cagggagcgt cgtcgtcgcg
     1801 cgagctgccg acccagtggc acgctgcgct cgagcgggcg gagcgttcga gcttcctgcg
     1861 cgagacgctc ggcgccggat tcctcgacgc ctatgtcgcc atcaagcgac aggaatgcga
     1921 gaagttcggc gcgctcgtga gcgatcacga ctacgagtgg ttcctcgaca ccgtctgatc
     1981 cacactcacg gctgtctcgt cgtcgtcgtc acgcgtgcgc tccggcgcat taagtcaccc
     2041 ggaacaccgt cgtagtgaga gctgagtcaa cgtgggcgcg ggcgtgcgcg cggagaatcc
     2101 gcgcagtcct cccgctcctc cccgagggac cttccgtgac ccgactctcc gcccgtacga
     2161 tcgcccgcag tgccgcactc tcgagcagga gctcgcccgt gaccatccac ctgcgcaaga
     2221 tgtacgtcga ctgccgattc gggcagctgc acatgcacac cgcgttcccg tcgagcggtg
     2281 gcttcgatga gctcaccccg ctcgtctgcc tctcgccgcc gtcgcagacg gggcgcctgt
     2341 atcgtccgct gcttcgcgac ctgggacgcg accgcagcgt ctatgcgccc gacctgcccg
     2401 gggctggcga gtcggacgca cccgatcatc cgccggccgt cgcggacttc gccggcgcct
     2461 gctgcgacct gctcgactcg ttgcggctca agcatgtgga cgtgatcgcc taccaggccg
     2521 gctcgctcgc ggcgatcgaa ttcgcgctcg cccggcccca gcaggtgcgg cgcgtcgcat
     2581 tgatcggcgt gccgtcgtcg gacgccggct accaggtcgg ggaacggttg ccgctgctgc
     2641 gacagcccgt gatggtcatg cggtcccggg acgagtactg ggaatccacc ggtcgcaccg
     2701 agccgttgct gcgcgaggcg cggcgcgtgg aactgcagca cccgagtggc gccgtgttcg
     2761 aggctggcgc ggaagaggtc atccgggtgg cgcgggactt cttcgacagg gcctgacggc
     2821 cctggaggaa gtggtcagtg gtcagtggtc agtggctagt gatcacagtg gacatcgagg
     2881 atgacttcct ccactagcca ctagccactg accacttctc actgtctctc ccacacgtac
     2941 gcgcacatca cgccggcgga gacggccgct acgaggcctt ccgccgcgag caccgccacg
     3001 gcgccgcggt ggtgacgcgc cttgatcgcc agggtgccga gcgtcgggag cgcgcccgcc
     3061 gcgaacagca gcccaccgta gatcgcaccg atcacgtgcc agggcgtcga cacgaccgcc
     3121 acgagccccc agctgagcca gaacagcagc cccgcggccg cgccgcacag cccgtaaaga
     3181 acggccccgg cgagcaggcg cgcggctgtg tcgggttgcc acgccgccgg tcgtaacgcg
     3241 ggccagcgcg cgaacagcgg cgccgagacg agccctgacc agagcgtgcc gacgagcccg
     3301 ccggcgatgc cggcgagcat catcatgtag agctcgatgc gcaagcggtg gattcctgga
     3361 ggccgcgggc gactcagacg tgtcgctgca cggcggccac ggcgcggtcg acgtcgatgg
     3421 cgtcgttggt gggatgcacg ctgatgcgga tcgcgtcgaa cccggcaccg tgcgtgacgc
     3481 gcccgagcac gatccggtcc tcacgcgcga tgcgctcgac gatctcgccc gggtcgcggc
     3541 ccggcaggcg cacgctgagg atgccggcat tgagcgaaaa gtgcgggggc gtgaccacgt
     3601 cgacgccggc gatgcgctgc aggccggacc gcagctgcga tccgagttct cgaatgcggc
     3661 cgtagacgag tgggcgcgag atcgcgtcct ggaactcgaa cgcgagcggg atcgcggcga
     3721 cggacgcgcc gcgacgcacg cgcgcgttgc cgaacctggt tgcggccgcg tactcgaacg
     3781 gcggcaccgg aagcgcagcg ttgaagcggt cggtgccgtt ccagccgtcc gcccgatcgg
     3841 gcagcgccgg ccacacgcgc gcctgcgcgt cccggcgcac gtacagcaga cccgcatcga
     3901 gcggcccgtt gagccagcga tcgacgccgg tcgcatacgc gtcgcagccg agatccgcca
     3961 gccggaagtc gacgtgcccg ctcgcgagcg cgccgtcgac gacggtgaac acgccgcgac
     4021 tgcgcgcgac cgcgcagagt tcgcggatcg gcagcaccgt accgtcgcag tcctgtatgt
     4081 gcgcgaggca cgcgacgcgc gtgcgcggcg tcagcgaacg ctcgaactgc tggacgatcg
     4141 cttccggcgt cgccgtgctg gcggccgttg cgggtgcctc gacgacccga atgccgcgac
     4201 ggcgtgcctg cgcgatccac ggatacaccg cggcggcatg gtcgtgcatg gacaccagca
     4261 cttcgtcgtc gcgctgcagg tcgaggcccg cagccaccag cgcgagcccg gtgcgcgcac
     4321 cgtcggtgag cacgagttcg cccgcgtcgc agccgagaaa gcttgcggcg gccgcgagca
     4381 ccgaccgctc gcccgccgag ccggcgccgt tcaacgcctc gaaggagcgg aagtccagcg
     4441 actgctcttc gagctggcga taggtgcgca ccagcaccgc gcgcagcgtc gggccgaacg
     4501 aggcggtgtc gagccacgtc agcccgggct cgagcaccag ctgtgcgcgt gcccacgccc
     4561 agcgatagcg gtcgatcggc ggcacgccct gcggcgtata tgtctgcgca ttcgcgaccg
     4621 gacccagcgc ggccgcggtg ccgagcacgc ccagcaacgt gcgcctggaa atcgggggag
     4681 ccatgatggg aaggatgtta acgtacgccg tgtcgggcct gacaacgcca tgcaagaaag
     4741 ttacatcgaa cggatcctca aggcgcgcgt ctacgacgtc gccatcgaat cgccgctcga
     4801 acctgcacga cgattgagcc ggcggctcgg gaaccaggtc ttcttcaagc gtgaagacct
     4861 gcagccggtg ttctcgttca agctgcgcgg cgcgtacaac aagatctcga cgctcgacga
     4921 ggcgacggcc cggcgcggcg tgatctgcgc gtcggcgggc aaccacgccc agggggtcgc
     4981 gctcgcggcg cgccggcgcg gcatcggagc ggtcatcgtg atgccgcgca ccacgcccgg
     5041 catcaaggtg caggccgtgc tcgacctcgg cggtgacgtc gtgctgcacg gtgacgactt
     5101 cgatcacgcg tacgagcacg cgctcggtct cgcggcggag agagggctca cgttcgtcca
     5161 tccgttcgac gacccggacg tgatcgccgg ccagggcacg gtcggcatgg agatcctgcg
     5221 ccagtgtccg acgcgccccg atgcggtgtt cgtgccggtg ggcggcggcg ggttgatcgc
     5281 cggcattgcg gcctacgtga agtcgctgta tccgcgcgtg cgcatcgtcg gcgtcgagcc
     5341 cgaggacgcc gcgagcatgc acgattcgct ggcccagggc gaacgcgtca cgctcgaccg
     5401 tgtcggcatc ttcgccgacg gtgtcgcggt gcggcgggtc ggcgaggaga cgttccggct
     5461 cgcgcgccag tacgtcgacg aggtggtgct cgtcggcacg gacgaaatct gcgcggcgat
     5521 ccaggacatc ttcgaggaca accgcacgat cgccgagccg gccggcgcgc tcgccgtggc
     5581 cggcatcaag cgctacgtcg cgcgcgagaa ctgccgcgag cagacgctcg tcgccgtgaa
     5641 cggcggcgcc aacatcaact tcgaccggtt gcggcacgtc gccgagcgcg ccgacatcgg
     5701 cgcgcagcgc gaggcactgc tcgccgtcga gatccccgag gagccgggca gtttcctgcg
     5761 tttctgccgg ctgctggggc ggcgcagcgt cacggagttc aactaccgct acgccgacgc
     5821 gcgttcggcg cggatcttcg ccggcgtcgc gctggccgag ggacgccgcg agaaggaggc
     5881 gctgctcgaa ctcgtcgccg aggccgggta ccgcgtgctc gacatgagcg acgacgagat
     5941 ggcgaagctg cacgtgcgct acatggtggg cgggcacgct cgcgggctgg cgcacgagcg
     6001 cctgttccgc ttcgagtttc ccgagcggcc cggtgcgctg ctcaagttcc tcgagtccat
     6061 cggggtcaac tggaacatca gcctgttcca ctaccgcaat cacgggtcgg attacgggcg
     6121 cgtgctggcc ggggtgcagg ttcccccggc cgacgacgcc gagttcagac gtcacctcgc
     6181 cgagttgcag tacgcctata ccgacgagac gctcaacccg gcctaccgca tgttcctggg
     6241 cgactgaacg tgggccaagg gcggcgcgcc tgagacgaag att
//
LOCUS       DASPFR010000292         4318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1181574, whole genome shotgun sequence.
ACCESSION   DASPFR010000292 DASPFR010000000
VERSION     DASPFR010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181574"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..541)
                     /gene="queD"
                     /locus_tag="VD737_07735"
     CDS             complement(179..541)
                     /gene="queD"
                     /locus_tag="VD737_07735"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HXW10488.1"
                     /translation="MTVEIFKAFTLECAHRLPNVAAGHKCARVHGHSFRIEVHVAGPV
                     DPTLGWVMDFAEVKTAFEPLFERLDHRYLNDVPGLENPTSENLARWIWRELQPALPRL
                     ARVVVHETCTSGATYTGE"
     gene            complement(538..975)
                     /locus_tag="VD737_07740"
     CDS             complement(538..975)
                     /locus_tag="VD737_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10489.1"
                     /translation="MKHAPACRRLLAVIASGTLLGTVALADPPADKGKSAGAAQSVKV
                     DGKSSSAAQDRRRDDGQGKADDNHGQVVSDCNHRANQKELKGKDRQDYVEWCTDRGER
                     HQYDDRRYDQDRSCYRKAAEKGLSGDSRRAYIQDCLRKQEKNR"
     gene            complement(972..3257)
                     /gene="rlmKL"
                     /locus_tag="VD737_07745"
     CDS             complement(972..3257)
                     /gene="rlmKL"
                     /locus_tag="VD737_07745"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: protein motif:HMM:NF008748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HXW10490.1"
                     /translation="MTAPDDEAPPGAVDSRPEHTFFAACPRYVPDLLATELRGLGIAV
                     EREHPAGVGFRGALRDAYLACLHSRTASRVVMTIADVEASDPDRMYRGLLELPWESHL
                     RADGTLAVDVVGESPGWLRNTQFAAVRAKDAIVDRLREQTGARPSVDVASPDLRVSLR
                     FARERVTVGLDLSGEPLHRRGYRQAGVEAPLKENLAAALLLRAGWREIASDGGHFFDP
                     MCGSGTLVIEAALIAANVAPGLLRRRFGFERWLGHDARMWESLRSSAEGHRDLDAVPA
                     GRCAGSDRDQDAIRASLANANRAGLGNHLVFERRDLSNLPATTHERGLVLVNPPYGVR
                     IGAPELEALYALLGEKLLRCFPGWEGAVFTGEPSLGRALRLRAYRTHTFFNGPLEGRL
                     LRFHLDDGAVEPDPAAVRAARLEAARTRPGAVMFGNRLRKNLSHLEKWARRQDVTCYR
                     AYDADMPEYAFAVDLYHAEERWVYVQEYAAPDSVAQDAARARRDEAIATIPGVFDVPP
                     ERMVLRVRRRQKGGEQYEKLDAEARFHVVREGPYRFLVNLTDHLDTGLFLDHRLTRAR
                     VGELAQGQRFLNLFAYTGTATVYAAGGGATASTSVDMSKTYLDWAVRNMDLNGLRAPR
                     HEFVQADCLAWLDAQVRATQPSKYDLVFIDPPTHSRSKRMEREFDVQRDHAALLRTAS
                     RLLAPDGVIVFSNNYQKFRLDPAVGEDFDVDDITRSTIPEDFARNPRIHVCFVLRPKT
                     RAVLAVAHARLDTGFPDGGQR"
     gene            complement(3254..>4318)
                     /locus_tag="VD737_07750"
     CDS             complement(3254..>4318)
                     /locus_tag="VD737_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXW10491.1"
                     /translation="TVRLSRMLYAEHPRHNLDALMERFQLTCTARHRALGDAQVLPAL
                     LAAIERQRGEPAMDEAVVEASREPRLPPHLPPELADDLPDAPGVYLFRDEHDAVLYVG
                     RSRNVRSRVFDHFTGSHRSQRHSELTTQVRKVEWTETGGELGALLLERRLLRELAPAG
                     NRLLRDADEECVVRLVRSGDGLAPQIVRLDQVVLDESDPVFGPFRTDRDARRALEGKA
                     REAGLCLKVLGLESGEGSCVAYQMQRCRGACVGREPRPLHDARLQLALASLRLKAWPF
                     QGPIGIRERAPHARGTCVHILDRWRHLGTARDEREVAEVLRQEVEPGFDTDGYRIIAR
                     ALKGVRLQDLLTFERPGFGR"
BASE COUNT          653 a         1511 c         1470 g          684 t
ORIGIN      
        1 ggtactcgac caagtactgg acgatgccgg aactgttccg ttacgcgcgt gacaagctgc
       61 acgtcgacta catgttctgg gtgcgcatcc cgaagtccac gcccctggac tcgtacaact
      121 ggaatcacgc gctgccggtc atcgagatga atccgcgctt ctgaagcgcc ggtcggcctc
      181 actcgcccgt gtacgtcgcg ccgctcgtgc aggtttcgtg cacgacgacg cgggcgagtc
      241 gaggcagcgc gggctggagc tcgcgccaga tccagcgggc gaggttttcg ctggtggggt
      301 tctcgagtcc cggcacgtcg ttgaggtaac ggtgatcgag ccgctcgaac agcggctcga
      361 acgccgtctt gacctccgcg aaatccatga cccagcccag cgtcgggtcc accgggccgg
      421 cgacgtgcac ctcgatgcga aacgagtgtc cgtggacccg ggcgcacttg tggccggcgg
      481 ccacgttcgg caaccggtgc gcgcattcga gcgtgaacgc cttgaagatc tccacagtca
      541 gcggttcttc tcctgcttgc gcaggcagtc ctggatatag gcgcgcctgg agtcgccgct
      601 gaggcccttc tccgcggcct tgcgatagca cgagcgatcc tggtcgtagc gacggtcgtc
      661 gtactggtgg cgctcgccac ggtccgtgca ccactcgacg taatcctgcc ggtccttgcc
      721 cttgagctcc ttctggttcg cgcgatggtt gcagtcggag acgacctggc cgtgattgtc
      781 gtcggccttg ccctggccgt catcgcgacg gcgatcctgc gcggcagaag acgacttgcc
      841 gtcgaccttg accgactggg cggcgcctgc gctctttccc ttgtccgcgg gcgggtcggc
      901 cagcgcgacg gttccgagca gcgttccgga cgcgatcacc gcgagcagac gcctgcaagc
      961 cggggcgtgt ttcatctctg acctccgtct gggaatccag tatcgagtcg ggcgtgcgcc
     1021 acggcaagca cggcgcgcgt cttcggacgc agcacgaagc agacgtggat gcgcgggttc
     1081 cgcgcgaaat cctccggaat cgtcgagcgc gtgatgtcgt ccacgtcgaa gtcctccccg
     1141 acggccgggt cgagcctgaa cttctggtag ttgttcgaaa agacgatcac gccatcgggg
     1201 gccagcaacc tggacgcggt ccggagcagc gcggcgtgat cccgctgcac gtcgaactcg
     1261 cgctccatgc gcttggagcg tgagtgcgtc ggcgggtcga tgaagaccag gtcgtacttc
     1321 gatggctgcg tcgcgcggac ctgcgcgtcg agccacgcga ggcagtcggc ctgcacgaac
     1381 tcgtggcggg gcgcgcgcag gccgttcagg tccatgttgc gcacggccca gtccaggtag
     1441 gtcttcgaca tgtcgacgct ggtggaagcg gttgcgccac cgccggccgc atacacggtc
     1501 gccgtccccg tgtacgcgaa caggttcagg aaccgctgcc cttgcgccag ctcgcccacc
     1561 cgggcgcgcg tgagacggtg atccaggaac agccccgtgt cgagatgatc cgtgaggttg
     1621 accaggaaac gatacggacc ctcgcggacg acgtgaaagc gggcctcggc gtccagcttt
     1681 tcgtactgtt cgccgccctt ctgccgacga cgcacgcgca gcaccatccg ctccggcgga
     1741 acgtcgaaca cgcccggaat cgtggcgatg gcctcgtccc tgcgcgcgcg ggcggcgtcc
     1801 tgagcgacac tgtccggcgc cgcgtactcc tggacgtaga cccagcgctc ctccgcgtgg
     1861 tacaggtcga ccgcgaacgc gtactccggc atgtcggcgt cgtaggcgcg gtagcacgtg
     1921 acgtcctggc gtcgcgccca cttctcgagg tgcgacaggt tcttgcgcag ccggttgccg
     1981 aacatcaccg caccgggcct cgtcctggca gcctccagcc tggcggcgcg caccgcggcg
     2041 ggatccggct cgaccgcgcc atcgtcgagg tggaaacgca gcagcctccc ttccagcggg
     2101 ccgttgaaga acgtgtgcgt gcggtatgcg cgtagcctga gcgcacggcc gagcgagggc
     2161 tcgcccgtga acacggcgcc ctcccagcca ggaaagcacc gcaacagctt ctcacccagc
     2221 aacgcgtaca gtgcttcgag ctccggcgcc ccgatgcgga cgccgtaggg tggattgacg
     2281 agcacgagtc cgcgctcgtg cgtcgtggca ggcaggttcg acaggtcgcg acgctcgaag
     2341 accaggtgat tgccgagccc cgcgcgattg gcattcgcga gcgaggcacg gatggcgtcc
     2401 tggtcgcggt cgctgccggc acagcggcct gcgggcaccg cgtcgagatc gcggtggccc
     2461 tccgcgctcg aacgcaggga ctcccacata cgtgcatcgt gaccgagcca gcgctcgaat
     2521 ccgaagcggc gtcgcagcag gcccggcgca acgttcgcgg cgatcagcgc cgcttcgatc
     2581 accagcgtgc cggacccgca catcgggtcg aagaagtggc cgccgtcgga ggcgatctcg
     2641 cgccaccctg cgcgcagcag caacgcggcg gcgaggttct ccttgagcgg agcctccacg
     2701 cccgcctggc gatagccgcg ccggtgcagc ggttcgccgc tcaggtcgag cccgacggtc
     2761 acgcgctcac gcgcaaagcg caggctgacc cggaggtctg gactggcgac gtccaccgac
     2821 ggcctcgcgc ccgtctgctc gcgcagtcga tcgacgatcg cgtccttcgc cctgaccgcg
     2881 gcaaactgcg tgttccgcag ccagcctggc gattcgccca cgacgtcgac cgcgagcgtg
     2941 ccatcggcgc gcaggtgcga ttcccaaggc agctccagca gcccgcggta catccggtcc
     3001 ggatccgatg cttcgacgtc cgcgatcgtc atcacgacgc gactggccgt gcgcgagtgc
     3061 aggcacgcga ggtaagcgtc gcgcagcgct ccgcgaaaac cgacgcctgc cgggtgttcg
     3121 cgctcgacgg cgatgccaag gccgcgcaac tcggttgcga gcaggtcggg aacgtagcgt
     3181 gggcaggccg caaagaacgt gtgttccggg cgcgagtcga cggccccggg aggcgcctcg
     3241 tcgtccggcg ccgtcatcgg ccgaacccgg gtcgctcgaa cgtcaggaga tcctgcaacc
     3301 gcacgccctt gagcgctcgg gcgatgatgc ggtacccgtc ggtgtcgaag cccggttcga
     3361 cctcctgtcg cagcacctcg gccacctcgc gctcgtcgcg agccgtgccg agatgccgcc
     3421 agcggtcgag gatgtgcacg caggtcccgc gcgcgtgcgg cgcgcgctcc cggatgccga
     3481 tcgggccctg gaacggccag gccttcagtc gcagcgacgc gagcgcgagc tgcagccgcg
     3541 cgtcgtgcag cggtcggggc tcgcgcccga cgcaggcacc ccggcacctc tgcatctggt
     3601 aggcgacgca gctgccctcg cccgactcga gaccgagcac cttgaggcac agaccggctt
     3661 cgcgtgcctt gccttccagc gctctccggg cgtccctgtc ggtgcggaac ggaccgaata
     3721 cggggtcgct ctcgtcgagg acgacctggt cgagccgtac gatctgcggc gccagcccgt
     3781 cgcccgagcg caccaggcga acgacgcact cctcgtccgc atcgcgcagc aggcggttac
     3841 ctgcgggcgc gagctcccgc agcaagcgcc tctcgagcag cagcgcaccg agctcgccgc
     3901 cggtctcggt ccattcgacc ttgcgcacct gcgtcgtgag ttccgagtgc cgctgagagc
     3961 gatggctgcc cgtgaaatgg tcgaacacgc gactgcgcac gttgcggctc cggcccacgt
     4021 acagcaccgc gtcgtgttcg tcgcggaaca ggtacacgcc cggcgcatcc ggcaggtcat
     4081 cggccagttc agggggcagg tgcggcggca ggcgtggctc acgcgatgcc tcgacgaccg
     4141 cctcgtccat cgccggctcg ccgcgttgcc gctcgatggc cgcgagcagc gcgggcagca
     4201 cctgtgcgtc gccgagcgca cggtgtcgcg cggtgcacgt gagctggaat cgttccatca
     4261 gcgcgtcgag gttgtgccgt gggtgctcgg cgtacagcat ccgcgacagt cgcaccgt
//
LOCUS       DASPFR010000293         4031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1791495, whole genome shotgun sequence.
ACCESSION   DASPFR010000293 DASPFR010000000
VERSION     DASPFR010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791495"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1085)
                     /locus_tag="VD737_07755"
     CDS             complement(114..1085)
                     /locus_tag="VD737_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10492.1"
                     /translation="MAVVATSSLLPLSADAYTTTRKFHPGHYSVILPVHNWQQKYMDD
                     AVRPGMRGIMKRYPWRLFEPTQGNYNFSAIQSDLNWAQAYGMQLIIMIEDKSFSSGRP
                     NPGYLDSISPRNQSGGYTMARWHPTYVTRYKALVTALGKRFDSHPNFEGIVQQESALT
                     LGSTTLKQYGYTPEKYRDALIASLGHALNVMPRSRVFWYQNFLVGNNNYIGSVAAALG
                     PKGLVMAGPDTLPDRKSLVEKSYPFFGQFKDKMHLGIQVEGSCYRALHETSGYSTKYW
                     TMPELFRYARDKLHVDYMFWVRIPKSTPLDSYNWKHALPVIEANPRF"
     gene            complement(1349..2635)
                     /locus_tag="VD737_07760"
     CDS             complement(1349..2635)
                     /locus_tag="VD737_07760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXW10493.1"
                     /translation="MAALPHIDSYYAATAHAVPDRPALDADLDVDVCVVGGGIAGCST
                     ALHLAERGYRVVVLEARRVGWGASGRSGGQAIVGYSCPQSTLVAAVGREDARRMFEIS
                     VEAQELIRALVAKHAIDCDLHWGHLHTALKPRQVVELLEHRRELEEDYGYDGTRWLDG
                     EQVRAILRTDRYVGGLRDDRSGHLHPLNYTLGVAAAAESAGARICEGTEVVGLDHGAT
                     VRVRTQAGAVVRARHVALCCNAFIDARISKKLRDRIMPVETYIVATERLGQARIEALI
                     RENVAVSDVNFVLDYFRRSEDHRLLFGGRVSYSGRDALNTARATRVRMLKVFPQLADA
                     RIEYAWGGHVDISMSRAPDFGRLEPNVYYLQGFSGHGIALTGIAGRLVAETIAGQSER
                     FDLYTKLQHRNFPGGPAFRTPALMLAMLWFRLKDLL"
     gene            complement(2637..4007)
                     /locus_tag="VD737_07765"
     CDS             complement(2637..4007)
                     /locus_tag="VD737_07765"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HXW10494.1"
                     /translation="MSCVADFEPARAWLGRRPQVRYVDLLLADQMGIPRGKRVSVEEL
                     EGVYRDGLLLPASMFALDVLGGTVQSTGLGFDEGDADRVCLPIRGSLVPVPWLGEQVA
                     QLQVSMYEHDRSPFFGDPRHVLEAVVRRYAPLGLKPVMAVELEFYLVDRERTPAGCAQ
                     PPRQPLTGRREYKTQINSMQDMNEYSAVLAAIDDAARAQELPTGTVLAEYGPGQFEVN
                     LHHVDDPLLACDHAIRLKRLVKGVALQFGMEATFMPKPYRDHAGSGAHLHVSLVDASG
                     RNVFASDDPAGSVRLRHAIGGLAATMNDAMAICAPTANSYRRFQPEAYVPLNPSWSVN
                     NRGVAFRIPHGPPDSRRVEHRIAGADANPYLLAAIVLGGMHLGLEQQLDPGPVLAGNA
                     YRDNTPSIPLSWAEALAVFEHSAFVRECLGERFARLYAQTRRGEMMEFCSYVPALDYD
                     WYLTTS"
BASE COUNT          676 a         1318 c         1354 g          683 t
ORIGIN      
        1 cgctggatct ggcgcgagct ccagcccgcg ctgcctcgac tcgcccgcgt cgtcgtgcac
       61 gaaacctgca cgagcggcgc gacgtacgcg ggcgagtgaa gcaagtccgc gcctcagaag
      121 cggggattcg cctcgatcac cggcagcgcg tgcttccagt tgtacgagtc caggggcgtg
      181 gacttcggga tgcgcaccca gaacatgtag tcgacgtgca gcttgtcacg cgcgtagcgg
      241 aacagttccg gcatcgtcca gtacttggtc gagtacccgg aggtttcgtg gagcgcacga
      301 tagcagcttc cctcgacctg gataccgagg tgcatcttgt ccttgaactg gccgaagaac
      361 gggtaggact tctcgacgag ggacttgcga tcgggcagcg tgtccggtcc tgccatcacg
      421 agccccttcg ggccgagcgc cgctgccacg gaaccgatgt agttgttgtt gccgaccagg
      481 aagttctggt accagaacac gcgtgagcgc ggcatcacgt tgagcgcgtg gcccaagctc
      541 gcgatcaggg catcgcgata cttctccggc gtgtagccgt actgcttgag cgtggtggac
      601 ccgagcgtga gggcggattc ctgctgcacg atgccctcga agttgggatg gctgtcgaaa
      661 cgcttgccca gcgcggtgac caaggccttg tacctcgtca cgtaggtcgg gtgccaccgc
      721 gccatcgtgt acccgccgct ctggttgcgc ggcgagatcg aatccaggta accggggttc
      781 ggccgtcccg acgagaacga cttgtcctcg atcatgatga tgagctgcat gccgtaggcc
      841 tgcgcccagt tcaggtcgga ctggatcgcc gagaagttgt aattgccctg cgtgggctcg
      901 aacaggcgcc atgggtatct cttcatgatg ccgcgcatgc cgggccgcac tgcatcgtcc
      961 atgtatttct gttgccagtt gtgtacggga aggatcacgg aatagtgacc cgggtggaat
     1021 ttgcgcgtcg tcgtgtaagc gtccgcagac agcggcagca aagaagaggt tgcgacgacg
     1081 gccaaggcgg ccagctttag tcctttcagc atcatctcac tccatcatca gattcttacg
     1141 acaagccctt gcggctcgag gacggagcga gtgtggccat cgagaagcgg gcagtaacga
     1201 aaaagcctcc agatcggttg tttgatccgc gaaaaacctg tcgcaattcg gcgactcggg
     1261 cgctttcgcg cttgatcgat gaaaataact atgcaagcgc tgttcttatg tttgtccagc
     1321 gccgcgctga gctcgcacga gccttggctc agagcaggtc cttcagccgg aaccagagca
     1381 tcgcgagcat gagtgcgggt gtcctgaacg ccggtccacc cgggaagttc cggtgctgca
     1441 gcttcgtgta caggtcgaac cgctcggact gaccggcgat cgtctcggcg acgagcctgc
     1501 ctgcgattcc ggtgagcgcg atgccgtgcc cggaaaaacc ttgcaggtaa tagacattgg
     1561 gttcgagacg gccgaaatca ggggcacggc tcatcgagat gtcgacgtga ccgccccagg
     1621 cgtactcgat ccgcgcatcg gcgagctgcg ggaagacctt cagcatccgc acgcgcgtgg
     1681 cgcgggcggt gttcaacgcg tcgcgtcccg aatagctgac gcgcccgccg aacagcagcc
     1741 ggtggtcctc cgaccgccgg aaatagtcga gcacgaaatt gacgtccgac accgcgacgt
     1801 tctcacggat cagcgcctcg atccgtgcct gtccgagccg ctcggtcgcg acgatgtacg
     1861 tctccacggg catgatgcgg tcgcgcagct ttttcgagat gcgcgcatcg atgaacgcgt
     1921 tgcagcagag cgcgacgtgc cgcgcgcgaa cgaccgcgcc tgcctgcgtg cgcacgcgca
     1981 ccgtcgcgcc gtggtcgagc ccgacgacct cggtgccctc gcagatgcga gcacccgcgg
     2041 actccgccgc cgcggcgacg ccgagcgtgt aattgagtgg atgcaggtgc ccgctgcggt
     2101 cgtcacgcag tccaccgaca tagcgatccg tgcgcaggat cgcccgcacc tgttcgccgt
     2161 cgagccagcg cgtgccgtcg tacccgtagt cctcctcgag ctcgcgtcgg tgctcgagca
     2221 gttcgaccac ctgccgcggc ttgagcgcgg tatgcaggtg cccccagtgc aggtcgcaat
     2281 cgatcgcgtg cttcgcgacc agcgcgcgga tgagttcctg cgcctcgacc gagatttcga
     2341 acatgcggcg cgcgtcctcg cggccgaccg cggcgacgag cgtcgattgc ggacacgagt
     2401 agccgacgat cgcctggccg ccgctgcgac ccgacgcgcc ccaacctacg cggcgggctt
     2461 cgagcaccac caccctgtag ccgcgctcgg cgaggtgcag cgccgtcgag cagcccgcga
     2521 taccgccgcc gaccacgcac acgtccacgt cgaggtcggc atcgagcgcg ggtcggtccg
     2581 gcaccgcatg ggccgtcgcc gcgtagtacg agtcgatgtg tgggagggcg gccatgtcag
     2641 ctggtcgtca ggtaccagtc gtagtcgagc gccggcacgt aggagcagaa ctccatcatc
     2701 tcgccgcgac gggtctgcgc atagagccgc gcgaaccgct cgccgaggca ctcgcgcacg
     2761 aacgcgctgt gctcgaacac cgcgagcgcc tcggcccacg acagcggaat gctcggcgtg
     2821 ttgtcgcggt acgcattgcc ggccagcacc ggcccggggt ccagctgctg ctcgagtccc
     2881 aggtgcatgc cgccgagcac gatcgccgcg agcaggtatg ggttggcgtc ggcgccggca
     2941 atccggtgct cgacgcgacg gctgtccggc ggtccgtgcg ggatgcggaa cgcgacgccc
     3001 cggttgttga ccgaccagct cgggttgagg ggcacgtagg cttccggctg gaagcgtcga
     3061 taggagttcg ccgtgggcgc gcagatcgcc atcgcgtcgt tcatggtcgc ggcgagtccg
     3121 ccgatcgcgt ggcgcagccg tacgctgccc gccggatcgt cggacgcgaa cacgttgcgc
     3181 ccgctcgcat cgacgaggct cacgtgcagg tgtgcgccgc tgccggcgtg gtcccggtaa
     3241 ggcttcggca tgaacgtggc ctccatgccg aactgcagcg ccacgccctt gaccagtcgc
     3301 ttcagccgga tcgcgtgatc gcaggcgagc agcgggtcgt cgacgtggtg caggttgacc
     3361 tcgaactgcc ccggcccgta ctcggcgagc accgtcccgg tgggcaactc ctgcgcacgc
     3421 gccgcgtcgt cgatcgcggc gaggacggcc gagtactcgt tcatgtcctg catcgagttg
     3481 atctgggtct tgtattcgcg acgcccggtg agcggctgtc gtggtggctg cgcgcagccc
     3541 gcgggcgtgc gttcccggtc gacgaggtag aactcgagct cgaccgccat gaccggcttg
     3601 aggccgagcg gcgcgtagcg gcgcacgact gcctcgagca cgtggcgcgg atcgccgaag
     3661 aacggcgaac ggtcgtgctc gtacatgctg acctgcagct gcgcgacctg ctcgccgagc
     3721 cacggcaccg gcacgagcga gccgcggatc ggcaggcaga cgcgatcggc atcgccttcg
     3781 tcgaagccga ggccggtcga ctgcacggtg ccgccgagca cgtccagcgc gaacatggag
     3841 gcgggcagca gcagtccgtc gcggtaaacc ccctccagtt cctcgaccga gacgcgcttg
     3901 ccgcgcggaa ttcccatctg gtcagcgagc agcaggtcga cgtagcgcac ctgcgggcga
     3961 cgaccgagcc aggcacgcgc cggctcgaag tccgcgacac acgacatggg aaccccgttg
     4021 tttaggattt a
//
LOCUS       DASPFR010000294         4019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_204867, whole genome shotgun sequence.
ACCESSION   DASPFR010000294 DASPFR010000000
VERSION     DASPFR010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204867"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="VD737_07770"
     CDS             <1..1418
                     /locus_tag="VD737_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXW10495.1"
                     /translation="TGLWPCEQKSGSAAPASPPEPARASFPTSNDRIASGIASSLVLV
                     SFDMPFSVAGITERNYHGTGVIVDAERGLVVVDRNTVPVAPGDVQITFGGSVEVPGKV
                     EYVHPLHNLAIVSFDPALLDGSPLRAARFDATELSPGEPVTVVGLSADSRLRSLSTFV
                     AQVDDVSFPLSRTLQFREANLEVATLVNAPADFDGVIVGRDGRVRALWSTFATEGARD
                     SAQVSRGMPASVVLEAVEAARSGQPLFSLQAEFEAIPIAAARELGLPAAWVPKFESRN
                     PEHRQVLAVARVAAGSAAAGVLKEGDLLLAVNGETLNRFREVERATQAPRVRLTVLRD
                     GAEQALEVETAPLLGEDIDRLLLWSGAVLHAPHRAMTIQRAIPPEGVFVAYFSYGSPA
                     TRHGLYAGRRIIEVDGKPTPDLDAFIAAIQGRPDRSSVRLKTVTWNGSTGVTTLKLDQ
                     NYWPTYELRRTPTGWERRPIT"
     gene            1465..2499
                     /locus_tag="VD737_07775"
     CDS             1465..2499
                     /locus_tag="VD737_07775"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HXW10496.1"
                     /translation="MVTARLPQDEIDQAVAAMRDGEVVAFPTETVYGLGADAQNPDAV
                     HKIFELKGRPATHPLIVHIDHVRALERWALNVPAEAAALAEKFWPGPLTIVLRRAPAV
                     DLAITGGQDTVAVRMPAHPVAQQLLRAFGSGIVAPSANRYGRISPTRPEHVRDEFGDA
                     VRIVLDGGDCKIGLESTIVSCIDTVPRILRPGALTLTQLRTVVPSMQPGPDALAPRVP
                     GSDTKHYAPFTPLSIVAARTLEEVVGQLTENHEKVAVLATRPPRVANKFMTWINAGRR
                     ADVYARELYVNLRTLDKSGAREILVEEVPAGELWDAVRDRLRRAASAENVVSGDPDIA
                     ALRADLGQDA"
     gene            2505..3635
                     /locus_tag="VD737_07780"
     CDS             2505..3635
                     /locus_tag="VD737_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HXW10497.1"
                     /translation="MAHADELRALVAHHGTPLLVVDCDVVRRQYQALRRALPRVELYY
                     ALKPLPHPAVVSTLKSAGASFDVATAGEIRLVRAAGVPAGRCIHTHPIKTDDDIRAAL
                     RYGIRTFVADNPDEIQKFRRYRRRAELLLRVSFRDPTAVVDLSRKFGCEPAAVPRLLE
                     LAREAGVRVRGLSFHVGSQVGDPAKYVEAIGVCRELIEQARAMQLDTPEVLDIGGGFP
                     IGYRREPVPIGEFCRPIRQALRKLPRDLRVIAEPGRFIAGPAGTAIARVIGRAQREGR
                     WWYYLDDGMYGSYSGQLYDHATYPIEVLDREGPHHPSVLAGPTCDSIDVVREDMPLPE
                     LQIGDLVVGRLMGAYTWATSSEFNFIPRAKIVAINVRARARA"
     gene            3632..>4019
                     /locus_tag="VD737_07785"
     CDS             3632..>4019
                     /locus_tag="VD737_07785"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HXW10498.1"
                     /translation="MNVAVVDHVAGAARARGIALVVDVFRAFTVAPHAYAAGASRLIP
                     VAAVEDALALRQRLPDALLVGERHARRLPGFDAGNSPTEILALDLAGRPVVHTTHAGT
                     QGLVAATGADEVLTASLVNVSALVRYV"
BASE COUNT          557 a         1476 c         1406 g          580 t
ORIGIN      
        1 gcaccggcct ctggccgtgc gagcagaaga gcgggtccgc ggccccggca tcgccacctg
       61 agccggcgcg cgcctcgttt ccgacgagca acgaccggat cgcgagcggc attgccagct
      121 cgctggtcct cgtctcgttc gacatgccgt tctcggtggc cggcatcacc gagcgcaact
      181 atcacggcac cggcgtgatc gtggacgcgg agcgcggcct ggtcgtggtc gaccgcaaca
      241 ccgtgcccgt cgcgccgggc gacgtgcaga tcactttcgg cggttcggtc gaggtgccgg
      301 gcaaggtcga atacgtgcac ccgctgcaca acctggcgat cgtgtccttc gacccggcgc
      361 tgctcgacgg ctcaccgctg cgcgcggcgc ggttcgacgc gaccgaactc agcccgggcg
      421 agcccgtcac cgtggtcggc ttgagcgccg attcgcggtt gcgatcgctc tcgacgttcg
      481 tggcgcaggt cgacgacgtg tcgtttccgc tgtcccgcac gctgcagttc cgcgaggcca
      541 acctcgaggt cgcgacgctg gtcaatgcgc cggccgattt cgacggcgtc atcgtcggtc
      601 gtgacggacg cgtgcgcgca ctgtggtcga cgttcgccac cgaaggtgcc cgcgacagcg
      661 cccaggtgag ccgcggcatg ccggcctccg tcgtgctgga ggcggtcgag gcggcccgct
      721 cgggccagcc gctgttctcg ctgcaggcgg agttcgaggc gatcccgatc gcggccgcgc
      781 gcgagctcgg cctgccggcg gcgtgggttc cgaagttcga gtcgcgcaat cccgagcatc
      841 ggcaggtgct cgcggtggcg cgcgtcgcgg ccggctcggc cgcggccggc gtgctcaagg
      901 aaggcgacct gctgctcgcg gtcaatggcg agacgctcaa ccggttccgc gaagtcgagc
      961 gcgcgacgca ggcgccgcgc gtgcgcctca ccgtgctccg ggacggtgcg gagcaggcgc
     1021 tcgaggtcga gacggcgccg ctgctcggcg aggacatcga ccggctgctg ctctggtccg
     1081 gcgccgtgct ccatgcgcca caccgcgcga tgacgatcca gcgcgcgatc ccgcccgaag
     1141 gcgtcttcgt cgcctatttc tcgtacggct cgcctgccac gcggcacggg ctctacgccg
     1201 gccggcgcat catcgaggtc gacggcaagc ccaccccgga cctcgacgcc ttcatcgccg
     1261 cgatccaggg gcggcctgac cgctcgtccg tgcgcctgaa gacggtgacc tggaacggtt
     1321 ccacgggtgt cacgacgctg aagctcgacc agaactactg gccgacctac gaattgcgcc
     1381 gcacgccgac gggttgggaa cgacgcccga ttacctgagc gcgcaactgc accgcgacgg
     1441 gcgtttgcgt atgattcgcg ccctatggtg accgcccggc ttccacagga cgagatcgac
     1501 caggccgtcg cggcgatgcg cgacggcgag gtcgtcgcgt tcccgaccga aaccgtctat
     1561 ggcctcggcg cggacgcgca gaaccccgac gcggtccaca agatcttcga gctcaagggc
     1621 cgcccggcca cgcacccgct gatcgtgcac atcgaccacg tccgcgcgct cgagcgctgg
     1681 gcgctgaacg tgcccgcgga agcggcggcg ctcgccgaga agttctggcc gggcccgctc
     1741 acgatcgtgc tgcgccgcgc gcccgcggtg gacctcgcga tcaccggcgg ccaggacacg
     1801 gtcgcggtgc gcatgccggc gcacccggtc gcgcagcagc tgctgcgggc gttcggcagc
     1861 ggcatcgtcg caccgtccgc caaccgctac ggccgcatca gccccacgcg cccggaacac
     1921 gtgcgcgacg agttcggcga cgccgtgcgg atcgtgctgg acggcggcga ctgcaagatc
     1981 ggcctcgagt cgacgatcgt ctcctgcatc gacaccgtcc cgcggatcct gcggcccggc
     2041 gcgctcacgc tcacccagct gcggacggtc gtgccctcga tgcagcccgg ccccgacgcg
     2101 ctggcgccgc gcgtgcccgg cagcgacacc aagcattacg cgccgttcac gccgctcagc
     2161 atcgtcgcgg cgcgcacgct cgaggaagtc gtcgggcagc tgaccgagaa ccacgaaaag
     2221 gtcgcggtgc tggcgacgcg gccgccgcgc gttgccaaca agttcatgac ctggatcaac
     2281 gccgggcgcc gtgccgacgt gtacgcgcgc gagctctacg tcaacctgcg cacgctcgac
     2341 aagtcgggcg cgcgcgagat cctcgtcgag gaagtccccg ccggcgaact gtgggacgcc
     2401 gtgcgcgacc ggctgcggcg cgccgcctcg gcggaaaacg tcgtctcggg cgacccggac
     2461 atcgcggccc tgcgcgccga ccttgggcag gacgcctgac ggccttggcc cacgcggacg
     2521 aactgcgggc cctggtcgcg caccacggca cgccgctgct ggtcgtggac tgcgacgtcg
     2581 tgcgccgcca gtaccaggcg ctgcgtcgag cgctgcccag ggtcgagctg tactacgcgc
     2641 tcaagccact gccgcacccg gccgtcgtct cgacgctcaa gtccgcgggt gccagtttcg
     2701 acgtcgccac ggccggtgag atcaggctgg tgcgcgcggc cggcgtgcct gccgggcgct
     2761 gcatccacac gcacccgatc aagacggacg acgacatccg cgcagcgctg cgttacggca
     2821 tccgcacctt cgtcgccgac aatcccgacg agatccagaa gttccgccgg taccgccgtc
     2881 gcgccgagct gctgctccgg gtgtcgtttc gcgaccccac ggccgtggtc gacctttcgc
     2941 gcaagttcgg ttgcgaacct gcggcggtgc cacgcctgct agagctggca cgcgaagcgg
     3001 gtgtgcgcgt gcgcggcctc tcgttccacg tcggctcgca ggtcggcgac ccggccaagt
     3061 acgtcgaggc catcggcgtc tgtcgcgaac tgatcgagca ggcgcgagcg atgcagctcg
     3121 acacgcccga ggtgctcgac atcggtggcg ggtttccgat cgggtatcgc cgcgaaccgg
     3181 tcccgatcgg cgagttctgc cggccgatcc ggcaggcgct gcgcaagctg ccgcgggacc
     3241 tgcgcgtgat cgccgagccc ggccggttca tcgccgggcc ggccggcacc gccatcgcgc
     3301 gcgtcatcgg ccgcgcgcag cgcgagggcc gctggtggta ctacctcgac gacggcatgt
     3361 acggcagcta cagcggacag ctctacgatc acgcgaccta tccgatcgaa gtgctcgatc
     3421 gcgaggggcc gcaccacccg agcgtgctcg ccgggcccac ctgcgacagc atcgacgtgg
     3481 tgcgcgagga catgccgctg cccgagctgc agatcggcga cctggtcgtg ggccggctga
     3541 tgggcgcata cacgtgggcg acctcgagcg agttcaactt cataccgcgc gcgaagatcg
     3601 tcgccatcaa cgtccgtgcc agggcccgcg catgaacgtc gcggtggtcg accacgtcgc
     3661 cggggccgca cgcgcccgcg gcatcgcgct ggtcgtcgac gtcttccggg cgttcacggt
     3721 ggcgccgcac gcgtacgcgg cgggggcgtc gcgcctgatc ccggtcgccg cggtcgagga
     3781 tgcgctggcc ctgcgccagc ggttgccgga cgccctgctg gtcggcgagc gccacgcgcg
     3841 ccggctgcca gggttcgatg cgggtaactc gccgaccgag atcctcgcgc tcgaccttgc
     3901 cggacggccg gtcgtgcaca cgacgcacgc gggcacgcag ggcctcgtcg ccgccacggg
     3961 ggccgacgaa gtgctgaccg cgtccctggt caacgtctcg gcgctcgtcc gctacgtcc
//
LOCUS       DASPFR010000295         5146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1304791, whole genome shotgun sequence.
ACCESSION   DASPFR010000295 DASPFR010000000
VERSION     DASPFR010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304791"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..752)
                     /locus_tag="VD737_07790"
     CDS             complement(159..752)
                     /locus_tag="VD737_07790"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HXW10499.1"
                     /translation="MKRHARGSGDAVLVIDVLNDFRFVEGAALLRRYRSRIAAVAALL
                     ARARAAGVPVVYANDNFGRWRSSVADVVDRLRESNPDVWRTLEPIRPEPNDYIVLKPR
                     HSAFYCTPLELLLEALDVDRLVLTGVSASSCIWFTGADAHVRGYAVVVPSDGVAALTA
                     PLERAVLELMRASLGAKTPQARGVRLRAPRRPRHAAG"
     gene            800..1285
                     /locus_tag="VD737_07795"
     CDS             800..1285
                     /locus_tag="VD737_07795"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HXW10500.1"
                     /translation="MNGPELHEIAIQAAKAVGYYATLLVMMRLAGKRLAGQTTTFDLL
                     VLITLGVTVQKVFLSEGTLETVVFVIVVFAVHRALASVCRLSPAVRHFVRGKPRALVV
                     DGVVIDAALATEGVSHDELLAGLRRLGFEQPTEVRLAVLEETGHISAVAMNDNGGELK
                     R"
     gene            complement(1299..1940)
                     /locus_tag="VD737_07800"
     CDS             complement(1299..1940)
                     /locus_tag="VD737_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HXW10501.1"
                     /translation="MTKLAPPGAATSGPYEAREFDLTAVTGLSQRAVELHLELYQGYV
                     KAFNTLLAEQSAAFPPAGEAAAPLDLASHARRFAFEYNGIVLHELFFEALGAPGATAL
                     DKQGVLGEAAERSFGGLEQWMQHAAELAKLRGIGWIVTVRERASNRIYNCWVDLHHLM
                     VPANTDVVLALDLWEHAYLLDFAPSQRSNYFEMLWNSIDWNKVEERCGVAERP"
     gene            2149..3573
                     /locus_tag="VD737_07805"
     CDS             2149..3573
                     /locus_tag="VD737_07805"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXW10502.1"
                     /translation="MGRWRLYGLITVLSVVFLASLFLMAEAGRTRLREASEQMQAATA
                     RRILVGELRQQVAEAALATRGFLLTGRLQYLEPLRTSGEGINRTAETFVLGYADEPAE
                     VAQAARQLRYLAGVQAGATLSVIALYGSRGPVAARELARAQAGGEDPLQRFMAVAERL
                     ERHEAARVAATRAQWQRESRLARGLGLAGALVNVVLVLSASAMAFGALRRHREGLEQI
                     ERRKDALESEAASRAAELNEVYGHLQTVQEQERSRLARGLHDELGGLLLAARMDATWI
                     RQHSRDAEPGALAGRIDRLLDVLDQGIDLKRRVIEELRPTLLDNMGLAAAIRWQVDEV
                     CARAQLRCEYHFPSQEPEVAPRTAIALFRVAQEALTNVQKHARASRVEVTLESAADYL
                     LLSIVDDGVGIGADAGNRRLSHGVAGMRHRVVALGGTLTVSSTPEGGTEVRAIVPRGA
                     VMDADPAPSSTPRASVARIMGQPP"
     gene            complement(3570..3809)
                     /locus_tag="VD737_07810"
     CDS             complement(3570..3809)
                     /locus_tag="VD737_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HXW10503.1"
                     /translation="MNWETFKGNWTQLRGRVRMQWGKLTDDQLDVIEGRRDLLLGKLQ
                     EAYGVTEEEADRQVQAWEDEQPAEEDAGPTAPRAG"
     gene            complement(3857..4363)
                     /locus_tag="VD737_07815"
     CDS             complement(3857..4363)
                     /locus_tag="VD737_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXW10504.1"
                     /translation="MQPPETTQHTGRPPSAETGVGPPALPPGEPAPLRARGQRETRRQ
                     PLRVLLVEDSPLLVQRITELVEDLPDVRLVGTAGSEAEAVGRLQADDVDVVILDLQLH
                     AGSGFGVLRTLQGRRNTAPAVVVFTNFAIGAYRDAAYSLGARRFLDKSRDYALLPAVL
                     RELSESLQ"
     gene            4662..>5146
                     /locus_tag="VD737_07820"
     CDS             4662..>5146
                     /locus_tag="VD737_07820"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HXW10505.1"
                     /translation="MKQATWTARAGLVIVALALGACSATRTQQSAGEVVDDSVLTAKV
                     KTALIEDPVTKAGQIDVETYRGVVQLAGFVDSNEAKARASELAKGVSGVQDVRNDLKV
                     GDADQSVGTKIDDTVITTQVKAKLIADSRTDAYKINVETNGGVVQLSGFVDNDEAKRT
                     AG"
BASE COUNT          768 a         1774 c         1860 g          744 t
ORIGIN      
        1 acgcagcgcg gcgggacgca cggccgcggc agcccgcggc ggacgcagca gccgcagcgg
       61 cgcccgcggg cgcggcggca gcaaacgtgg tcgcagcacc ggcggtcgcg gacgcggcgg
      121 cagccgttga cgcggccctg gcacgccgtg cggccggatc agcccgcggc gtgccggggc
      181 cggcgcggcg ctcggagcct gacgccgcgc gcctgcggcg tcttcgcccc gagcgatgca
      241 cgcatcagct cgagcacggc ccgctcgagc ggggccgtga gggcggcgac gccgtcggag
      301 gggacgacga cggcgtaccc gcgcacgtgc gcgtcggccc cggtgaacca gatgcagctc
      361 gacgcggaca cgcccgtgag cacgagccgg tccacgtcga gtgcctcgag cagcagctcg
      421 agcggcgtgc agtagaacgc cgagtgccgc ggcttcaaca cgatgtagtc gttcggctcg
      481 ggccggatcg gttcgagcgt gcgccagacg tccggattcg attcgcgcag ccggtcgacg
      541 acgtcggcga cgctcgaccg ccagcggccg aaattgtcgt tggcgtacac gaccggcacg
      601 cctgccgccc gcgcgcgcgc gagcagtgcc gcaaccgccg cgatccggga gcgatagcgt
      661 cgcagcagcg cggccccttc gacgaaccgg aaatcattca gcacgtcgat gacgagcacc
      721 gcgtcgccgc tgccccgcgc atggcgtttc atgggttacg cgtgcaaaca gtgcacacgg
      781 cttgcaagaa attcagcgca tgaacgggcc tgaactccac gagatcgcga tccaggccgc
      841 caaggccgtc ggctactacg cgacgttgct ggtgatgatg cggctggccg gcaagcgcct
      901 ggccggccag accaccacgt tcgacctgct ggtgctgatc acgctcggcg tgaccgtgca
      961 gaaggtgttc ctgagcgagg gaacgctcga gaccgtggtg ttcgtgatcg tcgtgttcgc
     1021 cgtgcatcgc gccctcgcgt ccgtctgccg cctgtcgccg gccgtgcgcc acttcgtgcg
     1081 cggcaagccg cgcgcgctcg tggtcgacgg cgtcgtgatc gacgccgcgc tggcgaccga
     1141 gggcgtctcg cacgatgaat tgcttgcagg actgcgccga ctcggcttcg agcagccgac
     1201 cgaggtgcgg ctggccgtgc tcgaggagac gggtcacatc tcggccgtgg ccatgaacga
     1261 caacggcggc gagctcaagc ggtagcggcc gcgaccggtt acgggcgctc cgccacgccg
     1321 caccgctcct ccaccttgtt ccagtcgatg ctgttccaca gcatctcgaa atagttcgag
     1381 cgctgcgacg gcgcgaagtc gagcaggtac gcgtgctccc agaggtcgag cgcgagcacg
     1441 acgtcggtgt tggccggcac catcaggtga tgcaggtcca cccagcagtt gtagatgcgg
     1501 ttggaagcgc gctcgcgcac cgtgacgatc cagccgatcc cccgcagctt cgccagctcc
     1561 gcggcgtgct gcatccactg ctcgagcccg ccgaaggaac gctcggccgc ctcgccgagc
     1621 acgccctgct tgtcgagagc ggtcgcgccg ggcgccccga gtgcctcgaa gaacagctcg
     1681 tgcagcacga tgccgttgta ttcgaacgcg aaacgccgcg cgtggctcgc gaggtcgagt
     1741 ggcgcggcgg cttcgccggc cggcgggaac gcggcgctct gctcggcgag cagggtgttg
     1801 aacgccttca cgtagccctg gtagagctcg aggtgcagct cgaccgcacg ctgcgacagc
     1861 cccgtcacgg cggtcaggtc gaattcgcgg gcttcgtacg gtccggaggt cgcggccccg
     1921 ggcggcgcca gcttggtcat cgggcaccgc cggtgcgcgc cgcgcagcgc gggttctggt
     1981 ctgggttcat tcgatggctc ctgggttggc cgggcggggt tgcccatgat gctcaaggag
     2041 gtctcgccgg ggcgtcggtc ccgcccggag ggcaactgta ggagccgaac ccgtcctatg
     2101 gacagccaag gaccgagcgc ttacaagcgg cgacatcgag gcaggcatat gggtcgctgg
     2161 cggctatacg ggctgatcac ggtactgtcc gtcgtgttcc tcgcgagcct gttcctgatg
     2221 gcggaggcgg ggcgcacgcg gctgcgcgag gcatcggagc agatgcaggc cgcgaccgca
     2281 cgccgcatcc tggtcggcga gttgcgccag caggtggccg aggccgccct cgcgacgcgc
     2341 ggcttcctgc tcacggggcg gttgcagtac ctcgagcccc tgcgcacctc gggcgagggg
     2401 atcaaccgca cggccgagac gttcgtgctg ggctatgccg acgaaccggc cgaggtcgcg
     2461 caggcggccc gccagttgcg ttacctcgct ggcgtgcagg caggcgccac cctctcagtg
     2521 atcgccctct acggttcgcg gggaccggtc gccgcgcgcg agctggcgcg cgcccaggcc
     2581 ggcggcgagg acccgctgca gcggttcatg gcggtggccg agcggctcga acgccacgag
     2641 gcggcgcgcg tcgcggcgac ccgcgcgcag tggcaacgcg agtcccggct cgcacgtggg
     2701 ctcggcctcg ccggcgcgct ggtcaacgtc gtgctcgtgc tgagtgcctc ggccatggcc
     2761 ttcggcgcgt tgcggcgcca ccgcgaggga ctcgagcaga tcgagcggcg caaggatgcg
     2821 ctcgagagcg aggccgcgag tcgcgcggcc gagctcaacg aggtgtatgg gcacctgcag
     2881 acggtgcagg aacaggaacg ctcgcgcctg gcgcgcgggc tgcacgacga actcggtgga
     2941 ttactgctgg ccgcgcgcat ggacgccacg tggatccggc agcattcgcg cgatgcggaa
     3001 ccgggcgcgc tcgcgggccg catcgaccgg ctgctcgacg tgctcgatca gggaatcgac
     3061 ctgaagcgac gcgtgatcga ggaactgcgg ccgaccttgc tcgacaacat gggcctggcg
     3121 gccgccatcc gctggcaggt cgacgaggta tgcgcgcgcg cgcagttgcg ctgcgagtac
     3181 cactttccgt cgcaggagcc ggaagtcgcg ccgcgcacgg ccatcgcgct gtttcgcgtc
     3241 gcgcaggagg cgctcaccaa cgtgcagaag cacgcccgcg cctcgcgcgt cgaagtcacg
     3301 ctcgaatctg ccgccgacta cctgctgctg tcgatcgtcg acgacggcgt aggcattggc
     3361 gcggacgcgg gcaaccggag gctgtcgcac ggtgtggccg gcatgaggca tcgtgtcgtg
     3421 gcgcttggcg gcacgctgac ggtgtcgtcg acgccggaag gcggcaccga ggtccgggcg
     3481 atcgtgcctc gtggagcggt gatggacgcc gatcccgcgc catcctcgac gccgcgggcg
     3541 tcggtcgcgc ggatcatggg gcagccgccc tagccggcgc gcggcgccgt cggcccggca
     3601 tcctcctcgg ccggctgctc gtcttcccag gcctgcacct gccggtccgc ctcttcctcg
     3661 gtgacgccgt aggcttcctg gagcttgccg agcagcaggt cgcgccggcc ctcgatcacg
     3721 tcgagctggt cgtcggtcag cttgccccac tgcatgcgca cgcgaccgcg caactgcgtc
     3781 cagttgccct tgaaggtttc ccagttcatc cgtgactccg gtggatcgag cggcgggctt
     3841 ctggcctgcc cgcaggttac tgcagcgact ccgacaactc ccgcaggacg gccggcagaa
     3901 gcgcgtagtc gcgcgatttg tcgaggaaac gtcgggcccc gagcgagtag gccgcgtccc
     3961 ggtaggcgcc gatcgcaaag ttcgtgaaca cgacgaccgc gggcgcggtg ttgcgtcgac
     4021 cctgcagggt gcgcagcacg ccgaacccgc tgcccgcgtg cagctgcagg tcgaggatca
     4081 ccacgtcgac gtcgtcggcc tggagccgac cgaccgcttc ggcctcggat cccgcggtgc
     4141 cgaccagccg gacgtcgggc aggtcttcga ccagctcggt gatgcgctgc acgagcagcg
     4201 gggagtcttc gaccagcagc acgcgcaacg gctgtcgtcg cgtctcgcgc tgcccacgcg
     4261 cgcggagcgg cgcgggctcg ccagggggca gcgctggcgg cccgacgccc gtctcggcgg
     4321 acggaggtcg ccccgtgtgc tgcgtcgtct cgggtggttg catgtcctgt tccgcacgac
     4381 gcagcctccg ggttgggaat gcccgtcgtg aaattgccct cgcaatgtat cgcccgcgga
     4441 cccccgacgg cgcgagcggt tgtccctccg caggccggtc gggagcttca cccagccgat
     4501 gtggggcggc tcctacgcca gggcggaccg ggcggctagc gcgaccgcgc aaccgcccga
     4561 tgcgctgccc gtccaaccgt aggcacgatg cagcccgtga tacgcggcct gaacccgtgt
     4621 ccaaggattg gattctttta ctggcaagga gagccatagt gatgaaacaa gcaacctgga
     4681 cggcccgcgc gggcctggtg atcgtggcgc tggccctcgg cgcctgctcg gctacgcgga
     4741 ctcagcagtc ggcgggcgaa gtcgtcgacg acagcgtcct gacggccaag gtcaagacgg
     4801 ccctgatcga ggatcccgtg accaaggcgg gccagatcga cgtcgagacc taccggggcg
     4861 tcgtgcagct cgccggcttc gtggactcga acgaagcgaa ggcgcgcgcg tccgaactcg
     4921 ccaagggcgt gtccggcgtg caggacgtcc gcaacgacct gaaggtcggt gacgccgacc
     4981 agtcggtcgg cacgaagatc gacgacaccg tgatcacgac gcaggtcaag gccaagctca
     5041 tcgccgactc gcggaccgac gcgtacaaga tcaacgtcga gaccaacggc ggcgtcgtgc
     5101 agctgagcgg cttcgtcgac aacgacgaag ccaagcgcac ggccgg
//
LOCUS       DASPFR010000296         4259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_693084, whole genome shotgun sequence.
ACCESSION   DASPFR010000296 DASPFR010000000
VERSION     DASPFR010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693084"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1605)
                     /locus_tag="VD737_07825"
     CDS             complement(<1..1605)
                     /locus_tag="VD737_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HXW10506.1"
                     /translation="MRRSSLRDHHHKASRDAEPAAGAWRTWLVGAMLTLGGIALVARA
                     VFLQVIDEDFLNRQGDARILRDVKLSANRGMVLDRNGEPLAVSTPVDTVWADPRKLAQ
                     HPQDFARLAKALDRDPQWLARRVTSSLEREFVYLVRHMRPQDAAKVKALGIPGVDTLR
                     EYRRYYPAGEVTGHLLGFTNVDDVGQEGVELAFDQWLGGEAGSMRVMRDREGRTIEII
                     ERTKAPQPGQDLRTSIDLRVQYLAYRALKQAVLENDARGGSVVVLDVATGEVLAMVNQ
                     PAFNPNDREQYLPSRYRNRATNDFFEPGSSIKPFVVAAGMKTGRFHADTLVDTGPGTF
                     RVGIKTVRDKHNLGMIDVTTVLAKSSNVGVVKIALSLKPQEMWTTLHDLGFGSVTGSG
                     FPGESAGILTGYEHWRSIGQATMSYGYGLSVTPLQLAQAYAVLGTGGIRRPVSLRRVD
                     TPPEGERVLEPAVVKELVRMMESVVSETGTARRAAVMGYRVSGKTGTAWKAANGGYNA
                     TKYMAVFGGLVPASAPRLAAVVVIDEP"
     gene            complement(1602..1877)
                     /gene="ftsL"
                     /locus_tag="VD737_07830"
     CDS             complement(1602..1877)
                     /gene="ftsL"
                     /locus_tag="VD737_07830"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HXW10507.1"
                     /translation="MTTSRWRVVVAVLWLAVLGSALGVVYGKQQARNRFNELQKLTTQ
                     RDDLDIEWGQLQLEQSTWATHGRVERVAHDELGMVTPAAADLRIVQP"
     gene            complement(1874..2803)
                     /gene="rsmH"
                     /locus_tag="VD737_07835"
     CDS             complement(1874..2803)
                     /gene="rsmH"
                     /locus_tag="VD737_07835"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HXW10508.1"
                     /translation="MAVHTPVLLDEVLAGLALQSAGRYCDATFGRGGHTAAILAQLGP
                     AGRVVAIDRDPEAVASGRERFAGESRLTLIRGSFGQLAGLLRAAGWSDGLHGVLLDLG
                     VSSPQLDEARRGFSFMQDGPLDMRMDYESGSSAAQWLARAGEREIADVLFRLGEERFS
                     RRIARALVAARAQAPITSTRRLADLVAAAVPTREPGKHPATRTFQAIRIHVNRELEEV
                     EAALPQAVDLLAPGGRLCVISFHSLEDRLVKRFMRREAQGDPVYAGLPDVPAHARPRL
                     KLVGGAVMPDEAEVGRNPRARSAVLRVAERIAA"
     gene            complement(2813..3268)
                     /gene="mraZ"
                     /locus_tag="VD737_07840"
     CDS             complement(2813..3268)
                     /gene="mraZ"
                     /locus_tag="VD737_07840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HXW10509.1"
                     /translation="MFRGANKVTLDAKGRVAMPARYRDTLVERSGGRLVATVDRADKC
                     LLIYPLPDWEEIERKLMRLPTLNPQARRLQRLMVGHATDLELDSHGRVLVPPKLREYA
                     GLSRQAMLIGQGSRFELWDEQHWNERRDEWLQAGDEAGELPAELESLSL"
     gene            complement(3689..4108)
                     /gene="rnpB"
                     /locus_tag="VD737_07845"
     ncRNA           complement(3689..4108)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VD737_07845"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          610 a         1475 c         1480 g          694 t
ORIGIN      
        1 cggctcgtcg atcacgacga ccgcggcgag ccgcggggcg ctcgccggca cgaggccgcc
       61 gaacacggcc atgtacttgg tcgcgttgta gccgccgttg gcggccttcc acgcggtgcc
      121 ggtcttgccg gacacgcgat agcccatcac ggccgcacga cgcgcggtgc ccgtctcgct
      181 gaccaccgat tccatcatcc gcacgagttc cttgaccacg gcgggctcca gcacgcgctc
      241 gccctcgggc ggcgtgtcga cgcggcgcag gctcaccggc cggcggatgc cgcccgtgcc
      301 cagcaccgcg tacgcctgcg cgagctgcag cggcgtcacc gacaacccat agccgtacga
      361 catcgtcgcc tggccgatcg atcgccagtg ctcgtagccg gtcaggatgc cggccgactc
      421 gccggggaag cccgaccccg tgaccgagcc gaagccgagg tcgtgcagcg tggtccacat
      481 ctcctgcggc ttgagcgaga gcgcgatctt gaccacgccg acattgctcg acttggcgag
      541 caccgtggtc acgtcgatca tgccgaggtt gtgcttgtcg cggacggtct tgatgccgac
      601 gcggaacgtg ccggggcccg tgtcgaccag cgtgtcggcg tggaaccggc cggtcttcat
      661 gcccgcggcc accacgaacg gcttgatgct cgagcccggc tcgaagaagt cgttggtcgc
      721 gcggttgcgg taccgcgacg gcaggtactg ctcgcgatcg ttggggttga acgccggctg
      781 gttcaccatc gccagcactt cgccggtcgc gacgtcgagc acgacgacgg agccgccgcg
      841 cgcgtcgttc tcgagcacgg cctgcttgag cgcgcggtag gccaggtact gcacgcgcag
      901 gtcgatgctc gtgcgcaggt cctggccggg ctgcggggcc ttggtgcgct cgatgatctc
      961 gatcgtgcgc ccctcgcggt cgcgcatgac gcgcatgctg ccggcctcgc cgccgagcca
     1021 ctggtcgaac gcgagctcga cgccctcctg cccgacgtcg tcgacgttgg tgaagccgag
     1081 caggtgcccg gtcacctcgc cagcagggta gtagcggcgg tattcgcgca gcgtgtcgac
     1141 gcccgggatg ccgagtgcct tgaccttcgc ggcgtcctgc gggcgcatgt gccggaccag
     1201 gtagacgaac tcgcgctcga ggctgctggt gacgcggcgc gcgagccact gcgggtcgcg
     1261 gtcgagcgcc ttcgcgagcc gcgcgaaatc ctgcgggtgc tgcgccagct tgcgcgggtc
     1321 ggcccagacc gtgtcgacgg gcgtgctcac ggcgagcggc tcgccgttgc gatcgagcac
     1381 catgccgcga ttggccgaca gcttgacgtc gcgcaggatg cgcgcgtcgc cctggcggtt
     1441 caggaagtcc tcgtcgatga cctgcaggaa caccgcgcgc gcgaccaggg cgatcccgcc
     1501 gagcgtcagc atcgcgccga cgagccaggt gcgccaggcg ccggcggcgg gctccgcgtc
     1561 gcgcgaggcc ttgtgatggt ggtcgcgcag cgacgaacgt ctcatggctg cacgatgcgc
     1621 aggtcggcgg ccgcgggcgt gaccatgccg agctcgtcgt gtgcgacgcg ctcgacgcga
     1681 ccgtgcgtcg cccaggtgct ctgctcgagc tgcagctggc cccactcgat gtcgaggtcg
     1741 tcgcgctgcg tcgtgagctt ctgcagctcg ttgaagcggt tgcgcgcctg ctgcttgccg
     1801 tagacgacgc cgagcgccga gccgagcacg gcgagccaga gcaccgccac caccacgcgc
     1861 cagcgcgagg tcgtcatgcg gcgatcctct cggcgacgcg cagcacggcg ctgcgcgcgc
     1921 gcggattgcg cccgacctcg gcttcgtccg gcatcacggc accgccgacg agcttcagcc
     1981 gcggccgcgc gtgggcaggc acgtcgggca agccggcgta gaccgggtcg ccctgtgcct
     2041 cgcgccgcat gaaacgcttg accagccggt cctcgagcga atggaagctg atcacgcaga
     2101 ggcggccgcc cggtgcgagc aggtcgacgg cctgcggcag cgccgcctcg acttcctcga
     2161 gctcgcggtt cacgtggatg cggatggcct ggaacgtgcg ggtggccggg tgcttgcccg
     2221 gctcgcgcgt cggcacggcg gcggcgacga ggtcggcgag ccggcgcgtc gacgtgatcg
     2281 gcgcctgggc gcgtgccgcg acgagcgcgc gggcgatgcg acgcgaaaac ctctcctcac
     2341 cgagcctgaa cagcacgtcc gcaatctccc gctcgccggc ccgcgcgagc cactgggctg
     2401 cactggatcc ggactcgtag tccatgcgca tgtcgagcgg accgtcctgc atgaaactga
     2461 agccccgcct ggcttcgtcg agctgcggcg acgagacccc gaggtccagc agcacgccgt
     2521 gcagcccatc gctccacccc gccgcccgga gcagccctgc gagctgcccg aacgaccccc
     2581 gtatcaacgt gagccgggat tcgcccgcga agcgctcgcg ccccgaggcc accgcctcgg
     2641 gatcgcgatc gatcgcgacg acgcgacccg cgggaccgag ctgcgcgagg atcgccgcgg
     2701 tatgaccgcc gcgcccgaac gtcgcgtcgc agtacctccc tgcggactgc aacgcgagac
     2761 cggccagcac ctcgtcgaga agcaccggcg tgtgcactgc caccgccggc gcctagagcg
     2821 agaggctttc gagttccgcg ggcagttcgc ccgcctcgtc acccgcctga agccactcgt
     2881 cccgacgctc gttccagtgc tgctcgtccc acagctcgaa gcgactgccc tgcccgatca
     2941 gcatcgcctg ccgcgacagg cccgcgtact cgcgcagctt cggcggcacc agcacgcgcc
     3001 cgtgcgagtc gagctcgagg tcggtcgcgt ggcccaccat gagtcgctgc aggcgtcgtg
     3061 cctgcggatt cagggtcggc agccgcatga gcttgcgttc gatctcctcc cagtccggga
     3121 gcggatagat cagcaggcac ttgtcggctc ggtccacggt ggccaccagc cggccgccgc
     3181 tgcgctccac cagggtgtcc cggtagcgcg ccggcatggc cacgcggcct ttggcgtcca
     3241 aggtgacttt gttggctccg cggaacatgt caggcgaggg tccgggcagt cagcccattt
     3301 catcccactt tttcccactc ggcgccacta taggcagcga ctggtcggag cgcaacggtt
     3361 gaaccgaaaa atttcgtagt acggtcagtg acttacactt atggtgaact gggtcacaga
     3421 tcttggttgt tgctttttga ggacaatcat cgacttaacc cactaacctt gacggaatct
     3481 tgaatgcccg cgatgggccc cgccgggcgg gacgggcagc ccgtggggga cccgcggccg
     3541 tgaccgcgcg gctcctgcac gcgcacccgt tacgcgaatt tgtgggacgg atcgcccttg
     3601 agcgccggcc gatcaacccg taccggttgg cagacctgtg ggcggaagtg ggcggggaac
     3661 ccgcgagtgg gtcgatatct cggaaaaagg cgggagtcgg ccgataagcc gggttctgtc
     3721 gcgggcaatc atccatctgg gacgcccgtc accgtgcgcc tcgagcgacc tacccggaag
     3781 cccctgcggg ccgcaggaac gggcccgagg gcccgcgcgc ttccctattt ggtcttgctc
     3841 caggtggggt ttgccgtgcc accgagtgtt gccaccggcg cggtgcgctc ttaccgcacc
     3901 gtttcaccct tgccgtccgc agcacttgcc gcgacggctc gaccggcgat gccggccggt
     3961 cccgtcacga acggctgcgt cttgggcggt ttgctctctg ttgcactttc cgtgggctcg
     4021 cgccccccag gcgttacctg gcaccttgcc ctatggagcc cggactttcc tccacgtcgt
     4081 gagacgcagc gattgcctgg ccgactcccg gggcgattat agaggaagtg gcgagtggtc
     4141 agtggtcagg ggttagtggt tagtggttag tggttagtgg tcagtggtca ggggttagtg
     4201 gttagtggtt agtggttagt ggtcagtggt cagtggtcag tgaggagcag aggacggtt
//
LOCUS       DASPFR010000297         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_693609, whole genome shotgun sequence.
ACCESSION   DASPFR010000297 DASPFR010000000
VERSION     DASPFR010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693609"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /gene="gcvPB"
                     /locus_tag="VD737_07850"
     CDS             <1..1176
                     /gene="gcvPB"
                     /locus_tag="VD737_07850"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HXW10510.1"
                     /translation="LSPVEHGQGFLACMHELQEMLKAVTGMKAVSLTPMAGAQGEFAG
                     VAMIRAYHVSRNEAHRNEIIVPDAAHGTNPATATMCGCTVREIPTLANGDIDLEALQK
                     VVGPQTAGIMLTNPSTIGVFERRIREVAGIVHGAGGLLYYDGANLNAILGKVRPGDMG
                     FDVIHMNLHKTFSTPHGGGGPGSGAVGVSERLLPFLPVPIVGRDGERYRWLDEQDVPQ
                     TIGRLSNFMGNTGVLLRAYVYMRMLGRDGMQRVSEFATLNANYLMARLRAAGFEVAYP
                     ERRASHEFIVTLRKEARDWDTNAMDFAKRLLDFGFHAPTTYFPLLVPECLLIEPTETE
                     SKEELDAFVDAMIAIRKEAQSEPEKLKGAPWTLPVRRLDDVRAAKQLDLTWKPVVSG"
     gene            1305..2237
                     /locus_tag="VD737_07855"
     CDS             1305..2237
                     /locus_tag="VD737_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HXW10511.1"
                     /translation="MPALICGSIAFDTVMVFQGRFREQILADRIHMLNVSFLVPSMRR
                     NFGGCAGNIAYNLRLLGEEGWPMATVGHDFEPYRRWLTACKVSLDFVRELDDEFTAQA
                     FITTDLDDNQITAFHPGAMSQSHLNAVPAGAGVTLGVVAPDGRAGMQQHAAQFAAAGI
                     PWLFDPGQGLPMFDGAELLAFIDKATWVAVNDYEGQLLQERTGLNAAQIAARVQAYIV
                     TRGAKGSTIHAGGREHAIPTARPEAIKDPTGCGDAYRAGLIFGLANELDWDTTGRIAS
                     LMGSIKIAHAGTQGHRFDMNEFEQRFRTAFGRSL"
BASE COUNT          392 a          778 c          762 g          357 t
ORIGIN      
        1 ctctcgccgg tggagcacgg ccagggcttc cttgcctgca tgcacgagct gcaggaaatg
       61 ctgaaagcgg tcacgggcat gaaggcggtg tcgctgacgc cgatggccgg cgcgcagggc
      121 gaattcgcgg gggtcgcgat gatccgcgcc tatcacgtgt cgagaaacga ggcgcatcgc
      181 aacgagatca tcgtgcccga cgcggcgcac ggcaccaacc ccgcgacggc gacgatgtgc
      241 ggctgcacgg tgcgcgaaat cccgacgctg gccaacggcg acatcgacct cgaggcgctt
      301 cagaaggtgg tcggcccgca gaccgcgggc atcatgctga cgaacccgtc cacgatcggg
      361 gtgttcgagc ggcgcatccg cgaagtcgcc gggatcgtcc acggcgcggg cgggttgctc
      421 tactacgacg gcgcgaacct gaacgcgatc ctcggcaagg tgcgccccgg ggacatgggg
      481 ttcgacgtga tccacatgaa cctgcacaag acgttctcga cgccgcacgg tggcggcggc
      541 ccgggctcgg gcgcggtcgg cgtgagcgaa cgactgctgc cgttcctgcc ggtgccgatc
      601 gtggggcgcg acggcgagcg ctaccgctgg ctcgacgagc aggacgtgcc gcagaccatc
      661 ggccggctct cgaacttcat gggcaacacc ggcgtgctgc tgcgcgcgta cgtctacatg
      721 cgcatgctcg ggcgcgacgg catgcagcgc gtctccgaat tcgcgacgct caacgccaac
      781 tacctgatgg cgcggctgcg cgcggccggc ttcgaggtgg cttatccaga gcgtcgcgcg
      841 agccacgagt tcatcgtgac gctgcgcaag gaggcgcgcg actgggacac caacgcgatg
      901 gacttcgcca agcgcctgct cgatttcggc ttccacgcgc cgacgaccta cttcccgctg
      961 ctcgtcccgg agtgcctgtt gatcgagccg accgagaccg agtccaagga agagctcgac
     1021 gcgttcgtcg acgcgatgat cgccatccgc aaggaggcac agtccgaacc cgagaagctc
     1081 aagggtgcgc cgtggacgct gcccgtgcgc cgcctcgacg acgtgcgtgc ggcgaagcag
     1141 ctggacctga cttggaagcc agtggtcagt ggttagtggc tagtggtcag taggaagcgc
     1201 cggcacgcgg cacgcacccg cgagcgcgcc cgcgcggcgg ccgacgcttc cgcatacact
     1261 ctctcactgg ccaccagcca ctagccacca gccacttcct cccaatgcct gccctcatct
     1321 gcggctcgat cgccttcgac acggtcatgg tcttccaggg tcggttccgc gagcagatcc
     1381 tcgcggaccg catccacatg ctgaacgtgt cgttcctcgt gccgagcatg cggcgcaact
     1441 tcggcgggtg cgcgggcaac atcgcgtaca acctgcggct gctcggcgag gagggctggc
     1501 cgatggccac ggtcggtcac gacttcgagc cgtaccgacg ctggctcacg gcctgcaagg
     1561 tgagcctcga cttcgtgcgc gagctcgacg acgagttcac ggcccaggcc ttcatcacga
     1621 ccgacctcga cgacaaccag atcaccgcgt tccatcccgg cgcgatgtcc cagtcgcact
     1681 tgaacgcggt gccggccggc gctggcgtca cgctcggcgt cgtcgcgccc gacggtcgcg
     1741 ccggcatgca gcagcatgcg gcccagttcg cggccgcggg gatcccgtgg ctgttcgatc
     1801 ccggccaggg cctgccgatg ttcgacggcg ccgaactgct cgctttcatc gacaaggcga
     1861 cgtgggtggc agtgaacgat tacgaaggcc agctgctgca ggagcgcacg ggcctcaacg
     1921 ccgcacagat cgccgcgcgc gtgcaagctt acatcgtcac ccggggcgcc aaaggcagca
     1981 cgattcacgc gggcggccgc gaacacgcaa tacccacggc gcggcctgag gcgatcaagg
     2041 atcccaccgg gtgcggcgat gcgtatcgcg caggcttgat ttttggcctc gccaatgaac
     2101 tcgactggga cacgacgggt cgtatagcgt cactgatggg ctcgatcaag atcgctcacg
     2161 ccggcacgca gggtcaccgc ttcgacatga atgaatttga acaacgcttc cgcacggcat
     2221 tcggccgcag cttgtgaaac ccttgcgcga ttgcattgat cgccgcgcgc tgcgtgcggc
     2281 ctggctcac
//
LOCUS       DASPFR010000298         2580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_205958, whole genome shotgun sequence.
ACCESSION   DASPFR010000298 DASPFR010000000
VERSION     DASPFR010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205958"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1638
                     /locus_tag="VD737_07860"
     CDS             115..1638
                     /locus_tag="VD737_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXW10512.1"
                     /translation="MARTLKESSRALDAIELRHCSVRLGRHWALREVTFAVRRGERWL
                     LLGPNGAGKTVLLKLLRGDLWPTPTGRGSRRYVYADGVADAEPVDAFDRVAYLGPEKQ
                     DRYERYESTLTVSQVVLTGFDDSDFPLAKPTAAQSRRIDALLGQAGLRSLAARQLLTL
                     SYGQRRRVLLARALVRRPDLLLLDEALNGLDENGRRAFLRALRRALAPGAAWILTSHR
                     RGDAQTVGATHLATLAHGRLERAGRIETVAHPPERSREGRRSPRSRRPRPYAARPLLR
                     LERAAIYRDGRAVIAPFDWTLDDGSHWLVRGTNGSGKSTLMALVYGDLWPRHGGTLQR
                     RWPAADDWKRRVGLVSPELQARYAATGCTVRDIVASGLHDSIGLNDFVTPAEARRVAR
                     ELSRWRMRELADRRARELSYGQLRLVLVARAFVRRRRLYLLDEPFDGLDVPARERLRD
                     RLDAAVQEEGATLVIVSHHADDVPDYVRCELTLRRGRAPTAAVRPAARPRTRSPRRR"
     gene            complement(1592..2503)
                     /locus_tag="VD737_07865"
     CDS             complement(1592..2503)
                     /locus_tag="VD737_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HXW10513.1"
                     /translation="MTQPRKCRLFLVDAFTRSRFCGNPAAVVLDADALDQQTLRRIGR
                     ELGGIETAFVFPSESNDYDVEIRFFSPRREVGFVGHATVAAHYVRAMVDGVPRGKVRQ
                     KSQAGIVEIEVTGTAPDLRVTIHQTPATFGPVVPEERLAPVLDALGISSASLHPGCPV
                     QVLSKANSRLLIGLQSPDTLASLQPKMERLVALTPHVGADGFFVFAMAPGSSPMTTES
                     RMFAPVLGVPEDPVSGNAHGMLGVYLLHHGLLQPVDGQASFVGHQGAYVDRPGQVEVA
                     VAASGRRATGVRVTGDAVIVYEAALPV"
BASE COUNT          371 a          907 c          940 g          362 t
ORIGIN      
        1 cgtctacgcc gacacgcccg tcgcgctgca cgggctcgcg agacactcgc tgctcgcgca
       61 cctgcagaag ctcgacgagg agggcctcgc cacccacgac ggcgatcggt ggacgtggcg
      121 cggaccctga aagagtcgtc acgcgcgctc gacgcgatcg aactgcgcca ctgcagcgtg
      181 cggctgggcc ggcactgggc attgcgtgag gtgacgttcg ccgtccggcg cggcgagcgc
      241 tggttgctgc tgggaccgaa cggcgcgggc aagaccgtgc tgctcaagct gctgcgggga
      301 gatctctggc cgacgccgac gggacgcggc tcgcgccgct acgtgtacgc ggacggcgtg
      361 gcggatgcgg agccggtcga tgcgttcgac cgcgtggcgt atctcgggcc cgagaagcag
      421 gatcgttacg aacgctacga gtcgacgctg acggtgtcgc aggtcgtgct caccggcttc
      481 gacgactccg acttcccgct cgcgaaaccg accgccgcgc aatcacggcg catcgacgcg
      541 ctgctcgggc aggcgggctt gcgcagcctc gccgcgcggc aactgctcac gctgtcgtac
      601 ggccagcgcc gccgcgtgct gctcgcacgg gccctggtac gacggccgga cctgctgctg
      661 ctcgacgagg cgctcaacgg cctggacgaa aacggacgtc gcgccttcct gcgggcactg
      721 cgtcgcgcgc tggcacccgg cgccgcgtgg atcctgacga gccatcgacg gggtgacgcg
      781 caaacggtcg gtgccacgca tctcgccacg cttgcccacg gtcggctcga acgtgcaggg
      841 cgtatcgaga ccgtcgcgca tccacctgag cgctcgcgcg agggccgacg aagcccacgc
      901 agccggcggc cacggccgta cgccgcgcgg ccgctgctgc gcctggaacg cgcggcgatc
      961 taccgcgacg ggcgggccgt gatcgcgccg ttcgactgga cgctggacga cggctcgcac
     1021 tggctcgtgc gtggtacgaa cggttcgggt aagtcgacgc tgatggcgct cgtctacggc
     1081 gatctctggc cgcggcacgg cggcacgctg cagcgacgct ggcctgcggc cgacgactgg
     1141 aaacggcgcg tcgggctcgt ctcgcccgag ttgcaggcgc gctatgcggc gaccggctgc
     1201 acggtgcgtg acatcgtcgc gtcgggcctt cacgacagca tcggtctcaa cgacttcgtc
     1261 acgccggcgg aagcgcgccg cgtggcacgt gagctgtcgc gctggcggat gcgcgagctg
     1321 gccgaccggc gagcgcgtga gctgtcgtac ggacagttgc ggctggtgct ggtcgcgcgc
     1381 gcgttcgtcc ggcgtcgccg gctgtatctg ctcgacgagc cgttcgacgg cctcgacgtc
     1441 ccggcgcgcg agcgcctgcg cgaccgactc gacgcggcgg tgcaggagga gggcgcgacg
     1501 ctcgtgatcg tgtcgcacca cgccgacgac gtgccggact acgtgcggtg cgagctcacg
     1561 ctgcggcgcg gtcgcgcgcc gacggccgcg gtcagaccgg cagcgcggcc tcgtacacga
     1621 tcaccgcgtc gccggtgacg cggacaccgg tcgcgcgcct gccgctcgcg gcgacggcga
     1681 cttcgacttg tcccggccga tcgacgtagg cgccctggtg cccgacgaac gacgcctggc
     1741 cgtcgaccgg ctgcagcagc ccgtggtgca gcaggtacac gccgagcatg ccgtgcgcgt
     1801 tgccgctgac ggggtcttcc ggcacgccga gcaccggcgc gaacatgcgc gactcggtcg
     1861 tcatcgggga cgaaccgggc gccatcgcaa acacgaagaa gccatcggcg ccgacgtgcg
     1921 gcgtcagggc cacgagccgt tccatcttcg gttgcagcga ggcgagcgtg tcgggcgact
     1981 gcaggccgat cagcaggcgg gagttggcct tgctcaggac ctgcacggga cagcccgggt
     2041 gcaggctcgc cgaactgatg ccgagcgcgt cgaggacggg ggcgagccgc tcttccggca
     2101 cgaccggccc gaacgtcgcc ggcgtctggt ggatcgtgac gcgcaggtcg ggtgccgtcc
     2161 cggtcacctc gatctcgacg atgcccgcct gtgacttctg ccgcaccttg ccacgcggca
     2221 cgccgtcgac catcgcacgg acgtagtggg ccgccaccgt cgcgtggcct acgaacccga
     2281 cttcgcgccg cggcgagaag aagcggatct cgacgtcgta gtcgttcgac tccgacggga
     2341 acacgaaggc cgtctcgatg ccgccgagtt cgcgcccgat ccgccgcagc gtctgctggt
     2401 cgagcgcatc ggcgtcgagc accacggccg cagggttgcc gcagaagcga ctgcgggtga
     2461 aggcgtccac caggaacaga cggcacttgc gcggctgggt catccggttg gtccaggggc
     2521 aacgaaggtg ctgcatagtg ccggaaccgc gtcctccgtc aacacgcgcg cgccgcaacc
//
LOCUS       DASPFR010000299         6573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1672270, whole genome shotgun sequence.
ACCESSION   DASPFR010000299 DASPFR010000000
VERSION     DASPFR010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672270"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            421..1431
                     /locus_tag="VD737_07870"
     CDS             421..1431
                     /locus_tag="VD737_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10514.1"
                     /translation="MDSTMQFRRSDYDVTNTVQVSSPGAVRHAVEELFQQTWPGVPYD
                     RLTQAFGDFERLFTGQYPGYLGCDTVYHDLQHSLDDTLAMARLLAGYERTHAADQRLG
                     ADRALLGLVVALFHDAGYIRQVGETQYRNGAEFTRTHVTRGAEFLTRYLPTVGLAHWV
                     PVATQIIHFTGYEVPFGEIRLDDPLDRRVGHLLGTADMMAQMSDRCYLEKCRDRLYPE
                     FVLGGVALGRENGGDLKVTYGSGLDVLRQTPKFFEETMRTRLDGEFAGAWRHFEALFD
                     GRNPYMEAIDRNLQHLDRVLRTGNWSLLRRAPPVFTWEKNPIQNVRSLVIDRLRTAVD
                     PA"
     gene            complement(1477..2946)
                     /locus_tag="VD737_07875"
     CDS             complement(1477..2946)
                     /locus_tag="VD737_07875"
                     /inference="COORDINATES: protein motif:HMM:NF006112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HXW10515.1"
                     /translation="MAALRRILLGLVLILVVLVAVVAVRTWTYRAPDVVAPVELATAP
                     PIDVERATTRLAEAVRLRTVSHQTPAENDVAEWDRLHAWLQSTYPAAHAAMTREVIAE
                     RTLVYTWTGREPALPAVVLLAHQDVVPVTPGTEGDWKHPPFDGVIAEGAVWGRGTIDD
                     KGSLVALFEALDALATAGFVPRRTVIVVSGHDEEAGGSGARAAAQHLRERDVRAEFAL
                     DEGYAVVDGLSLVQQPVALIGVGEKGYATLKVAAPAPGGHSSAPPPETGVETLARAVL
                     AITGAPFPLEFSGPAADMVRNLAPHAKPVVRMAVANEWLFRRLLVRQLASTPAGAASL
                     HTTIAPTMLRGSPKENVLPQDATAWINYRIAPGNTADDVMGRARRATDGLDVKLEWEG
                     GVAYDPAPVSSTQSAAYRLLATLASDGGRLPVAPALVTATTDSRYMVGVATDIYRFQP
                     IVIRLDELPMIHGTNEHLTIDNLRRLCEFYATLVATVAA"
     gene            complement(2971..3765)
                     /gene="trpA"
                     /locus_tag="VD737_07880"
     CDS             complement(2971..3765)
                     /gene="trpA"
                     /locus_tag="VD737_07880"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HXW10516.1"
                     /translation="MSATTAIAPRDTISTAIREAMRRDGRPAVVAFATAGFPDRAGFV
                     SNLKAIASEADVVEIGVPFSDPMADGATIQRASFAALRDGFTLPWLLGELEAMQPRTK
                     APLLLMSYLNPLLAFGLNRLPEAAASAGVSGFIIPDLPYEESAEIRAALDRVGVALVQ
                     MVTPVTPAPRLRMLCEASQGFVYAVTMTGTTGKSVAVPDEVLEYMDRVRAVSPVPVCA
                     GFGIRSRDQVVRMQGHVDGVVVGSALVEVLERGEDPAAFVRTLKGP"
     gene            complement(3762..5009)
                     /gene="trpB"
                     /locus_tag="VD737_07885"
     CDS             complement(3762..5009)
                     /gene="trpB"
                     /locus_tag="VD737_07885"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008171747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HXW10517.1"
                     /translation="MNIDSLQIDREALLAQTLQGAWPDARGRFGPFGGRYVPETLVPA
                     LDRLEQGIREILPDPSFQAELDRQLRTWVGRPTPLTFAAELSRRWGAEVWLKREDLAH
                     TGAHKINNAIGQALLAQRLGAKRVVAETGAGQHGVATAAACARLGLPCTVYMGSIDME
                     RQAPNVGRMKLLGAEVVPVTGGDQTLRAAIDEALRDWVADPDGTFYILGSAVGPHPYP
                     YLVREFQRVIGVEARAQMLAQSGALPDVVVACVGGGSNAIGLFHPFVGDLAVKLLGFE
                     AGGTGAGLGQNAASLAYGRPGVLQGCYSLLLQDEHGQIQETHSVSAGLDYPGVGPEHA
                     LLASIGRLDFAAAEDDESLAALAECCQAEGILPAIESAHAFAGARKYAARNPGSRILI
                     GLSGRGDKDMPTLQRTLLKGATK"
     gene            complement(5081..5725)
                     /locus_tag="VD737_07890"
     CDS             complement(5081..5725)
                     /locus_tag="VD737_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HXW10518.1"
                     /translation="MNDVPLWIKVCGLRTVEAVEAAVEAGADAVGFVFHAASPRNLEL
                     ETARSLAGVVPSSVARVAVFMHPAQALLDAAVEAVHPEWVQTDAGDLPRLRVPPGVRV
                     LPVYRTGAAIASESIRDRRFLLESARSGAGERADWNAAAAVPRVGEMVLAGGLDAGNV
                     GEAIATVRPFGVDVSSGVERQRGIKDPALIRAFVSTARKMGTLRISDGSREACK"
     gene            complement(5722..6549)
                     /locus_tag="VD737_07895"
     CDS             complement(5722..6549)
                     /locus_tag="VD737_07895"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HXW10519.1"
                     /translation="MGLLSDMAAGSARRSREAQAVASAAALREQCETLPQPRRVSLPE
                     QGFDLIAELKLRSPSLGDLSAQTLDPVGRLDGYARGGAAICSILTEPSRFDGALEHLR
                     TAAQALRPYGVPAMRKDFLVDPYQVLEARAHGAGGVLLIVRMVERARLVAMLDEAAAQ
                     GLFVLLEAFDADDLRVAGELARERAGREEQVLMGLNCRDLETLEIDFGRFERLRERMP
                     AAWPAVAESGVVTPEDAARVAALGYRLALVGTSLMRQADPAEAVSTLLQAGREAVPR"
BASE COUNT          946 a         2422 c         2260 g          945 t
ORIGIN      
        1 gtcctgctcc gcggcgccga cccgagcgcg gtggaggaga agtctatcgc gctgcgccgg
       61 ctggccgagc gctggcctga tcgccttccg tcggtcaagc aggactacga ggcccgcgcg
      121 ggaactcgac gggacggccg agacgttgcg caaccagctc gcgggttcgc accggagaac
      181 gggctcgacg gactgaccgg cgcgtttact gagtgctcgc ggaactgtgg cgcgcgtcac
      241 acgcccacgc gtggcgtcgc ctggcgcggc cgcgtcgccg cggcgtcgtc ttcgcaagtt
      301 ttgacatatg agtgcgaact cgcgcacgaa ttgccgcgat cgcttcgatg tcggtttttt
      361 tgactctggt aattgcactg caagggtcta gactccgccg gaaacaacga acggtccccc
      421 gtggactcga ccatgcagtt ccgccgcagc gactacgacg tcacgaacac cgtgcaggtg
      481 agttcgccgg gcgccgtgcg ccatgcggtc gaggagttgt tccagcagac gtggcccggc
      541 gtcccgtacg accggctgac gcaggcgttc ggcgacttcg agcggctgtt cactggccag
      601 tatcccggct acctgggctg cgacaccgtc taccacgacc tgcagcattc gctggacgac
      661 acgctcgcga tggcacgcct gctcgccggc tacgagcgca cgcacgcggc cgaccagcgc
      721 ctgggcgccg accgcgcgct gctcggcctc gtcgtcgcgc tgttccacga tgcgggctac
      781 atccgccagg tcggcgagac ccagtaccgc aacggggccg agttcacgcg cacacacgtc
      841 acccgtggcg ctgagttcct gacgcgctac ctgccgaccg tcgggctggc gcactgggtg
      901 ccggtcgcga cgcagatcat ccacttcacg ggttacgaag tacccttcgg cgagatccgc
      961 ctcgacgacc cgctcgaccg ccgggtcggg cacctgctcg gcacggcgga catgatggcg
     1021 cagatgtccg atcgctgtta cctcgagaag tgtcgtgacc ggctgtaccc ggagttcgtg
     1081 ctcggcggcg tcgcgctcgg ccgcgagaac ggcggcgacc tcaaggtgac ctacggctcg
     1141 gggctcgacg tgctgcggca gacgccgaag ttcttcgagg agacgatgcg gacccgactc
     1201 gacggcgagt tcgccggtgc ctggcgccac ttcgaggcac tgttcgacgg gcgcaacccg
     1261 tacatggaag cgatcgatcg caacctgcag cacctcgatc gcgtgctgcg caccggcaac
     1321 tggtcgctgc tgcgtcgcgc gccgccggtg ttcacctggg aaaagaaccc catccagaac
     1381 gtgcggtcgc tggtgatcga ccgcctgcgc acggcggtcg atcccgcctg accgattccg
     1441 ccgccgggcc tgcggcggta cgcctgccgt cgacgcctac gccgccaccg tcgcgaccag
     1501 cgtcgcgtag aactcgcaga gcctgcgcag gttgtcgatc gtcaggtgtt cgttggtgcc
     1561 gtggatcatc ggcagctcgt cgagccggat cacgatcggc tggaagcggt agatgtccgt
     1621 cgccaccccg accatgtacc ggctgtccgt cgtggccgtg acgagcgccg gcgccacggg
     1681 caggcgccca ccgtccgagg ccagcgtcgc gagcagtcgg tatgcggccg actgcgtcga
     1741 cgacaccggc gccgggtcgt acgcgacacc cccctcccac tcgagcttca cgtccaggcc
     1801 gtcggtcgcg cggcgcgcgc gccccatgac gtcgtcggcg gtgttgcccg gcgcgatgcg
     1861 gtagttgatc caggccgtcg cgtcctgcgg caggacgttc tccttcggac tgccgcgcag
     1921 catggtcggc gcgatcgtgg tgtgcagcga ggcggcgcct gcgggcgtgc tcgcgagctg
     1981 ccggaccagc agccgtcgga acagccactc gttcgccacc gccatccgca cgaccggctt
     2041 cgcgtgcggc gcgaggttgc gcaccatgtc ggccgcgggt cccgagaact cgagcggaaa
     2101 gggcgcgccg gtgatcgcaa gcaccgcgcg ggccagcgtt tctacgccgg tctccggcgg
     2161 cggcgcggag gagtgcccgc ccggcgcggg tgccgcgacc ttaagcgtgg cgtagccttt
     2221 ctcgccgacg ccgatcagcg cgacgggttg ctgcaccagc gagaggccgt cgacgacggc
     2281 gtagccctcg tcgagcgcga attcggcgcg gacgtcgcgc tcgcgcagat gctgcgccgc
     2341 ggcgcgcgcg cccgagcccc cggcctcctc gtcgtgaccg ctcacgacga tcaccgtgcg
     2401 gcgtggcacg aaccccgcgg tcgccagcgc gtcgagcgcc tcgaacaacg cgaccagcga
     2461 acccttgtcg tcgatggtgc cgcggcccca gaccgcgccc tcggcgatca cgccgtcgaa
     2521 cggcgggtgc ttccagtcgc cctccgtgcc gggcgtcacc ggcacgacgt cctggtgggc
     2581 cagcaggacg acggccggca acgcgggctc gcggccggtc cacgtgtaga cgagcgtgcg
     2641 ttcggcgatc acctcgcgtg tcatcgccgc gtgtgcggct gggtaggtcg actgcagcca
     2701 ggcgtgcagc ctgtcccatt cggcgacgtc gttctcggcc ggtgtctggt ggctcaccgt
     2761 gcgcagccgc acggcttccg ccaggcgggt cgtggcgcgt tcgacgtcga tgggcggggc
     2821 cgtcgcgagt tcgaccggcg cgacgacgtc cggcgcacgg tacgtccagg tgcgcacggc
     2881 gaccacggcc accagcacga ccaggatcag cactaacccg agcaggatcc gacgcagcgc
     2941 cgccatggtg cctccctccg gcccgtgtgc ttatggcccc ttcagtgtac gcacgaatgc
     3001 cgccggatcc tcgccccgct cgagcacctc gaccagcgcc gagcccacca cgacgccgtc
     3061 gacgtggccc tgcatgcgca cgacctggtc gcgactgcgg atgccgaacc ccgcgcacac
     3121 gggcacgggc gaaacggcgc gcacccggtc catgtactcg agcacttcgt cgggcacggc
     3181 gaccgacttg ccggtcgtgc ccgtcatcgt gaccgcgtag acgaacccct ggctcgcctc
     3241 gcagagcatc cgcagccgcg gcgccggcgt gaccggcgtg accatctgca ccagcgcgac
     3301 gccgacgcgg tcgagcgccg cgcgaatctc cgcgctttcc tcgtacggca ggtccgggat
     3361 gatgaagccc gagacgccgg cgctcgcggc ggcttccggc aatcggttca acccgaacgc
     3421 cagcagtgga ttcaggtagc tcatcagcag caacggcgcc ttggtacgcg gctgcatcgc
     3481 ctcgagttcg ccgagcagcc acggcaacgt gaagccgtcc ctgagcgcgg cgaagctcgc
     3541 gcgctggatc gtcgcgccgt cggccatcgg gtcgctgaac ggcacgccga tctccacgac
     3601 gtcggcctcg gacgcgatgg ccttcaggtt gctcacgaag ccggcgcggt cggggaagcc
     3661 cgccgtcgcg aacgcgacga ccgcgggacg accgtccctg cgcatggctt cgcggatcgc
     3721 cgtgctgatg gtgtcgcgtg gcgcgattgc cgtcgtggcg ctcatttcgt cgcccccttc
     3781 aacaacgtgc gctgcagcgt cggcatgtcc ttgtcgcccc gcccggagag cccgatcagg
     3841 atgcgagaac cggggtttcg cgcggcgtac ttgcgagcgc ccgcaaaggc gtgggccgat
     3901 tcgatcgcgg gcaggatgcc ttcggcctgg cagcactcgg cgagcgccgc gagcgactcg
     3961 tcgtcctcgg cggccgcgaa gtccaggcgg ccgatgctcg cgagcagcgc gtgctcgggc
     4021 ccgacgcccg ggtagtcgag ccccgccgac acagagtgtg tctcctggat ctggccgtgc
     4081 tcgtcctgca gcagcagcga atagcaccct tgcagcacgc ccggcctgcc gtaggcgagc
     4141 gaagcggcgt tctggcccag gcccgcaccg gtcccgccgg cctcgaagcc gagcagcttg
     4201 acggcgaggt cgccgacgaa cgggtggaac aggccgatcg cgttcgaccc gccacccacg
     4261 caggcgacga cgacgtccgg cagcgcgccc gactgcgcga gcatctgcgc gcgcgcctcg
     4321 acgccgatca cgcgctggaa ttcgcgcacg aggtacggat acgggtgcgg accgaccgcg
     4381 gagcccagga tgtagaacgt gccgtcgggg tcggcgaccc agtcgcgcag cgcctcgtcg
     4441 atcgcggcgc gcagcgtctg gtcaccgccc gtgacgggca cgacctcggc gccgagcagc
     4501 ttcatgcggc cgacgttcgg cgcctgtcgc tccatgtcga tcgagcccat gtagacggtg
     4561 cacggcagcc cgaggcgcgc gcaggcggcg gccgtcgcga cgccgtgctg gccggcgccc
     4621 gtctccgcga ccacgcgttt cgcgcccagc cgctgcgcga gcagcgcctg gccgatcgcg
     4681 ttgttgatct tgtgcgcgcc cgtgtgcgcg aggtcctcgc gcttgagcca gacctcggcg
     4741 ccccagcggc gcgagagttc ggccgcgaac gtgagcggcg tcggacggcc gacccacgtg
     4801 cgcagctggc ggtcgagctc cgcctgaaac gacgggtccg gcaggatctc gcggatgccc
     4861 tgctcgagcc ggtcgagcgc cggcaccagc gtctcgggca cgtagcgtcc gccgaagggg
     4921 ccgaagcgtc cgcgcgcgtc cggccaggcg ccctgcagcg tctgcgcgag cagcgcctcc
     4981 cggtcaatct gcagcgagtc gatgttcatc tctgcaccaa aaatggggac attccgaatt
     5041 tccggctaat tggggacatt ccgaataaac gtggcgaggc tcatttgcag gcctcgcgcg
     5101 agccgtcgga aattcggagt gtccccattt tccgggctgt gctgacgaag gcgcggatca
     5161 aggcggggtc cttgatgccg cgttgacgct ccacgcccga gctcacgtcg acgccgaacg
     5221 gtcgcacggt ggcgatcgcc tcgccgacgt tgcccgcatc gagtccaccg gcgagcacca
     5281 tctcgccgac gcgcgggacc gccgccgccg cgttccagtc ggcccgctcg cccgcgccgc
     5341 tgcgcgcgct ctccagcagg aaccggcggt cgcggatgct ttcggaggcg atcgcggcgc
     5401 ccgtgcgata caccggcagc acgcgcacgc cgggcggcac gcgcagccgt ggcaggtccc
     5461 cggcgtccgt ctgcacccac tccgggtgca cggcctcgac cgccgcgtcg agcagcgcct
     5521 gcgccggatg catgaacacg gcgaccctgg cgacgctcga cggcaccact ccggcgagcg
     5581 agcgcgccgt ctcgagctcg aggttgcgcg gcgacgcggc gtggaacacg aagccgacgg
     5641 catcggcgcc tgcctcgacc gccgcctcga ccgcctcgac ggtgcgcaga ccgcagacct
     5701 tgatccagag tggcacgtcg ttcatcgcgg caccgcctct cgaccggctt gcagcagcgt
     5761 gctcacggcc tccgccggat ccgcctgccg catcaggctc gtaccgacca gcgccagtcg
     5821 atagccgagc gccgcgacgc gcgcggcatc ctccggcgtg acgacgccgc tctccgcaac
     5881 cgcgggccac gcggccggca tccgctcccg cagccgctcg aaacgcccga agtcgatctc
     5941 gagcgtctcg aggtcgcggc agttgagccc catcagcacc tgctcctcgc gacctgcgcg
     6001 ctcgcgcgcg agctcgccgg ccacccgcag gtcgtcggcg tcgaaggcct cgagcagtac
     6061 gaacaagccc tgcgcagccg cttcgtccag catcgcgacc agccgcgcgc gctcgaccat
     6121 gcgcacgatc agcagcacgc cgcccgcgcc gtgggcacgc gcttcgagca cctggtacgg
     6181 gtcgaccaga aaatccttgc gcatcgccgg cacgccgtac ggccgcagcg cctgcgccgc
     6241 ggtgcgcagg tgctcgagcg cgccgtcgaa gcgcgacggc tcggtcagga tcgagcagat
     6301 cgccgcgcca ccgcgggcat agccgtcgag ccgccccacc gggtcgagcg tctgcgccga
     6361 caggtcgccg agcgacggcg agcgcagctt gagctccgcg atcaggtcga agccctgctc
     6421 cggcaacgag acccgccgcg gttgcgggag cgtctcgcac tgctcccgca acgcggctgc
     6481 gctcgcgacc gcctgcgctt cgcgcgagcg tcgcgcgctg ccggccgcca tgtcggacag
     6541 cagtcccatg attcaggtcg tccggcgggc cga
//
LOCUS       DASPFR010000300         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1548915, whole genome shotgun sequence.
ACCESSION   DASPFR010000300 DASPFR010000000
VERSION     DASPFR010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548915"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VD737_07900"
     CDS             <1..253
                     /locus_tag="VD737_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10520.1"
                     /translation="LRSEACSAVLGWANPREPQALRRLQLAAEQSHSLAVLFRPLQAG
                     LTPSPAVLRVALPGRESGGLQVRIVKSRGGRPATVHLGH"
     gene            363..1835
                     /locus_tag="VD737_07905"
     CDS             363..1835
                     /locus_tag="VD737_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HXW10521.1"
                     /translation="MHLPALMLDALGETVRTLPGAPPVAVVDLERNGKVVRACDERVR
                     AAGVHAGMSVNSALALVPTLNPIARNLRCEKQLLEAVARRGLHFTPRVSLEPPDAVVL
                     EVRGSLRLFGGARRLCERLEREIAATTGATVRFAITPTPLASLWLARLHAGAHPVLVR
                     SREELASRLAPLSLGCTRWPDKALQALATMGVRTVGECLRLPRDGFARRFGAEALAML
                     DRATGRLAEPRCAFAAHERFALRGDLEPEIGDSARLDGVLEPMLLELCRFLRERSRGV
                     QAIEVQLRHRQTPLTRVRLRFVQPIGAQPQRMQALVRERLARLELPEPVRSVRLLSGP
                     LLDLQSEPVELFAHDRRESGENLPQLVERLRARLGHEAVHGLACVAEHRPEAAWRKEP
                     SPGLRPNSPARAGEVREPSPALYEVQRPLWLLEAPEPCDATRLELEDGPECIESGWWD
                     GHDVARDYYVARNREGARLWIFRNRRDTDPAGWFLHGWFA"
     gene            1846..>2154
                     /locus_tag="VD737_07910"
     CDS             1846..>2154
                     /locus_tag="VD737_07910"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HXW10522.1"
                     /translation="MYAELHCLTNFTFLRGASRAEELVERAAQLGYAALAITDECSVA
                     GVVRAHVAAKDHGLALIVGAEFRLEDGLRCVLLAQDRAGYGRLCRLITRGRRSAPKGR
                     Y"
BASE COUNT          280 a          778 c          782 g          314 t
ORIGIN      
        1 gctgcggtcg gaggcgtgca gcgccgtact gggctgggcc aatccgcgcg agccgcaagc
       61 cctgcggcgc ctgcagctgg cggccgagca atcccacagc ctcgcggtgc tgtttcgccc
      121 gctgcaggcg gggctcacac cgtccccagc cgtcctgcgc gtcgccttgc ccgggcgtga
      181 gtcgggcggg ctgcaggtgc gcatcgtgaa aagccgcgga ggacgccccg ccacagtcca
      241 cctggggcac tgacgtggcc ccgctcgccc gacgccgggt tgcgccggca ctccgtccat
      301 cgctctcggt accggagcgt gccccgctcg ggcagccgcg tccgcgggaa ctctggttgg
      361 cggtgcacct gccggcattg atgctcgatg ccttgggaga gacggtccgg acgctgcccg
      421 gagcaccacc cgtcgcggtc gtcgacctcg agcgcaatgg caaagtcgtc cgagcctgcg
      481 acgagcgcgt ccgtgcggcg ggcgtgcacg ccgggatgtc ggtcaactcg gcgctcgcgc
      541 tcgtgcccac gctgaatccg atcgcgcgca acctgcgttg cgaaaagcaa ctgctcgaag
      601 cggtggcgcg acgcgggctg cacttcacgc cacgcgtgag cctcgagccc cccgacgccg
      661 tggtgctcga ggtgcgcggc agcctgcggc tgttcggcgg tgcgcgacgg ctctgcgagc
      721 gactcgagcg cgagatcgcc gcgacgaccg gcgccacggt gcggttcgcg atcacgccga
      781 cgccactcgc gtcgttgtgg ctcgcgcgcc tgcacgccgg cgcgcatccc gtgctcgtgc
      841 gcagccgcga ggaactggcg agccgtctcg cgccgctgtc gctcggctgc acgcgctggc
      901 ccgacaaggc gttgcaggca ctcgcgacga tgggagtgcg cacggtcggc gagtgcctgc
      961 gcctgccccg ggacggcttc gcgcgtcgtt tcggggccga ggcgctggca atgctcgatc
     1021 gcgccaccgg tcgcctggcc gagccgcgct gcgcgttcgc cgcgcacgag cggttcgcgt
     1081 tgcggggcga cctggagccg gagatcggcg acagcgcgcg actcgacggc gtgctcgagc
     1141 ccatgttgct cgaactctgt cgtttcctgc gcgaacgcag ccgcggcgtg caggcgatcg
     1201 aggtgcagct gcgtcaccgg cagacgccgc tcacgcgcgt gcgcctgcgt ttcgtgcagc
     1261 cgatcggcgc gcagccgcaa cgcatgcagg cgctggtgcg cgagcggctc gcccggctcg
     1321 agctgccgga gcccgtccgc agcgtgcgac tgctgagcgg accactgctc gacctgcagt
     1381 cggagcccgt cgaactcttt gcgcacgacc ggcgcgaatc cggcgagaac ctgcctcagc
     1441 tggtcgagcg gctgcgtgcg cggctcggac acgaagccgt gcacggcctg gcctgtgtcg
     1501 cggagcaccg acccgaggcc gcctggcgaa aagagccctc acccggcctg cggccgaact
     1561 ctcccgccag ggcgggagag gtgagagagc cctcaccggc actgtacgag gtgcaacggc
     1621 cgctctggct gctcgaggct cccgagccct gcgacgccac ccgtctcgaa ctcgaggacg
     1681 gccccgagtg catcgagtcg gggtggtggg acggccacga cgtggcgcgc gattactacg
     1741 tggcccgcaa ccgtgagggc gcgcggctct ggatctttcg caaccggcgc gacaccgacc
     1801 ccgcggggtg gttcctgcac ggctggttcg cctgagcatg caggcatgta cgccgagctg
     1861 cactgtctca ccaacttcac cttcctgcgc ggtgcctcgc gcgcggagga gctggtcgaa
     1921 cgggctgcgc agctcggtta cgcagcgctc gcgatcaccg acgagtgctc cgtggccggc
     1981 gtcgtgcgtg cgcacgtcgc agcgaaggac cacggcctcg cgctgatcgt cggcgccgag
     2041 ttccggctcg aggacggact gcgctgcgtg ctgctcgcgc aggaccgtgc agggtacggg
     2101 cggctctgcc gcctgatcac tcgcggtcgc cggtcggcac ccaagggcag gtac
//
LOCUS       DASPFR010000301         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1060730, whole genome shotgun sequence.
ACCESSION   DASPFR010000301 DASPFR010000000
VERSION     DASPFR010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060730"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VD737_07915"
     CDS             <1..1176
                     /locus_tag="VD737_07915"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020359689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HXW10523.1"
                     /translation="QRTVHGKPLAYLDNAASSQRPRAVLDAMTRYYETSHANVHRGVH
                     TLSQEATDLFEGAREKARRFINARSTKEIVFVRGTTEAVNLVAQSYGRPRFGPGDEIL
                     ISWLEHHANIVPWQLLCQQTGATLKVAPITARGEIDFDAYAALLSSRTRLVALAHVSN
                     ALGTVVPVPRFIQAAKSRGIPVLLDGAQAVPHLKVDVQALDCDFYAFSSHKMCGPTGI
                     GVLYGRESLLREMPPWQGGGDMILAVTFEKTVYNDLPYKFEAGTPAIAEAVGLGAAID
                     YLEHLGMERIAAAEHRLLEYANARLQEVPGVRFVGTAPDKAAVVSFTLDGVHPHDLGT
                     ILDHEGVAIRTGHHCAMPVMDFFKVPATARASFAFYNTREEVDQLVAALHTAREMLG"
     gene            1176..1619
                     /locus_tag="VD737_07920"
     CDS             1176..1619
                     /locus_tag="VD737_07920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HXW10524.1"
                     /translation="MDLKDLYRDIIVDHNRHPRNFREMPDADRHADGFNPLCGDKLTL
                     YVKLDGERIADVSFHGSGCAISVASASLLTESVKGRTVPEAEKLFGQMHQLLTRDDAD
                     VDVPSLGKLGALSGVREFPARVKCASLCWHTLDAALHSQERVSTE"
     gene            1632..2183
                     /gene="sufT"
                     /locus_tag="VD737_07925"
     CDS             1632..2183
                     /gene="sufT"
                     /locus_tag="VD737_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HXW10525.1"
                     /translation="MYRVDNEPVTFTRDVQAIIVPAGLEVKLRQGQQGYITQALGGSF
                     TVYVEGNLFRIAGKDADALGKEPDEVPELPPNANDEDIRRVAWEQMKGCYDPEIPINI
                     VDLGLVYDCDVKQEDDGGRVVSVTMTLTAPGCGMGEVLVADVREKVEVIPTVKRADVE
                     LVFDPPWNQSMMSEAARLQTGMM"
BASE COUNT          429 a          839 c          899 g          372 t
ORIGIN      
        1 cagcggacgg tgcacggcaa gccgctcgca tacctcgaca acgcagcgtc gagccagcgt
       61 ccccgcgccg tgctcgacgc gatgactcgc tactacgaga cgtcgcatgc caacgtacac
      121 cggggcgtgc acacgctgag ccaggaggcg acggacctgt tcgagggcgc ccgcgagaag
      181 gcccggcgct tcatcaacgc tcgctcgacg aaggagatcg tgttcgtgcg cgggacgacc
      241 gaggcggtca acctggtggc gcagagctac gggcggccgc ggttcggccc gggggacgaa
      301 atcttgatct cctggctcga gcaccacgcc aacatcgtgc cctggcagct gctgtgccag
      361 cagacgggcg cgacgctcaa ggtcgcgccg atcacggcgc gcggcgagat cgatttcgac
      421 gcctacgcgg ccctgctgtc ctcccgcacg aggctcgtcg cgctggcgca cgtctcgaac
      481 gcgctcggca ccgtagtgcc cgtgccgcgc ttcatccagg cggcgaagtc gcgcggaatc
      541 ccggtgctgc tcgatggcgc gcaggccgtg ccccacctca aggtcgacgt gcaggcgctc
      601 gactgcgact tctatgcgtt ttcgtcgcac aagatgtgcg gcccgaccgg catcggcgtg
      661 ctgtacggcc gcgagtcgct gctgcgcgag atgccgccgt ggcaaggcgg cggcgacatg
      721 atcctcgcgg tgacgttcga gaagacggtc tacaacgatc tgccgtacaa gttcgaggcg
      781 ggcacacccg cgatcgccga ggccgtcggc ctgggcgccg ccatcgacta tctcgagcat
      841 ctcggcatgg agcgcatcgc ggcggccgag caccggttgc tcgagtacgc caatgcgcgg
      901 ctgcaggaag tgccgggcgt ccggtttgtc ggtacggcgc cggacaaggc cgcggtcgtg
      961 tcgttcacgc tcgacggcgt gcacccgcac gacctcggca cgatcctcga ccacgagggc
     1021 gtcgcgattc gcacgggcca ccactgcgcg atgcctgtga tggacttctt caaggtgccg
     1081 gcgacggccc gtgcctcgtt cgcgttctac aacacgcggg aagaggtcga ccagctggtc
     1141 gcggccctgc acacggcacg ggagatgctc ggctgatgga cctcaaggac ctctatcgcg
     1201 acatcatcgt ggaccacaac cggcatccgc ggaatttccg cgaaatgccg gacgccgacc
     1261 ggcacgcgga cggcttcaac ccgctgtgcg gcgacaagct gacgctgtac gtcaagctcg
     1321 acggcgagcg catcgccgac gtgtcgttcc acgggagcgg ctgcgcgatc tcggtcgcct
     1381 cggcctcgct gctcacggaa agcgtcaagg gccggacggt gccggaggcg gaaaagctgt
     1441 tcggccagat gcatcaactg ctgacgcgcg acgatgcgga cgtcgacgtg cccagcctcg
     1501 gaaagctcgg cgcgctctcg ggcgtccgtg agtttccggc gcgcgtgaag tgcgcgagcc
     1561 tctgctggca cacgctcgac gccgcgctgc acagccagga aagggtttcg acggaatagc
     1621 tggcgaccac catgtacaga gtcgacaacg agccggtcac gttcacgcgg gacgtgcagg
     1681 cgatcatcgt cccggcgggc ctcgaagtga aactgcggca gggccagcag ggctacatca
     1741 cgcaggcgct cggcggcagc ttcacggtct acgtcgaggg caacctgttc cgcatcgccg
     1801 gcaaggacgc ggacgcgctg ggcaaggagc cggacgaggt cccggagctg ccgcccaacg
     1861 ccaacgacga ggacatccgt cgcgtcgcgt gggaacagat gaagggctgt tacgacccgg
     1921 agatcccgat caacatcgtc gacctcgggc tggtctatga ctgcgacgtc aagcaggaag
     1981 acgacggcgg ccgcgtggtg tcggtgacga tgacgctcac ggcgccgggc tgcggcatgg
     2041 gcgaggtgct ggtcgccgac gtgcgggaga aggtcgaggt gatcccgacg gtcaagcgcg
     2101 ccgacgtgga actggtgttc gatccgccgt ggaaccagtc gatgatgtcc gaggcggcgc
     2161 ggctgcagac ggggatgatg tgacgcgatg agggcggccg cggcgactca ggcggcgccg
     2221 gatcgcgccg gacgtgggga ccagatctgg gtcgccgggc tgtgcgaggc atggatgccg
     2281 agcccagagc ccccagggac gggtttacgg cgtcccggcg acccagatgt gggcccagcg
     2341 tccgcttgcg cgagccaggc gccggaccgc tgcgacgtgc atcggggcat ccaagcatga
     2401 gcacctggat ccgcgtcgcg ccagccgcgg ccatcgcgcc gggcgactac gcctcggccg
     2461 aggtcgatgg cgtgttcgtc gcggtctaca acatcgacgg cgagttcttc gcgatcgacg
     2521 acgtctgcac gcacgacgg
//
LOCUS       DASPFR010000302         4363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1672859, whole genome shotgun sequence.
ACCESSION   DASPFR010000302 DASPFR010000000
VERSION     DASPFR010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672859"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1448)
                     /gene="gcvPA"
                     /locus_tag="VD737_07930"
     CDS             complement(72..1448)
                     /gene="gcvPA"
                     /locus_tag="VD737_07930"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HXW10526.1"
                     /translation="MPFIPHTDADVRAMLDAIGAPDIDALFEEIPAELRSPGLPGIPP
                     AMTEMEVGRLMAELAARDGRPLNFIGAGAYEHHIPAAVWAIVTRGEFYSAYTPYQAEA
                     SQGTLQLLYEYQTMMASLTGLDVSNASLYDGASGLAEACLMAVRANRRSKSQRILVPS
                     AVNPTYRDVAQAICGNQNLAFESVPFDARSGQTVLAALDQYRGQDITAVVVQQPNFFG
                     TLEDVDAVTNWAHENGALAIGVVNPTSLAVLKPPGEWGARGADIACGEGQPLGVPLSS
                     GGPYFGFMAARAEHVRQMPGRIIGRTLDLDGRTGFTLTLQAREQHIRRSKATSNICTN
                     QGLLVTAATIYMSLLGPQGLAQVAATCIQRTQQLVDALTRIPGVRLAFDHPRFHEAVL
                     LLDRPVRPVLEALARRGIVGGVDISARYPQLGNALLVCATETRTVGDVETYAEALAET
                     MRSKAAAA"
     gene            complement(1577..1972)
                     /gene="gcvH"
                     /locus_tag="VD737_07935"
     CDS             complement(1577..1972)
                     /gene="gcvH"
                     /locus_tag="VD737_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HXW10527.1"
                     /translation="MSNVPADLRYLKSHEWARLENDGTITIGISDHAQQALGDLVFVE
                     APEPGRRVNAGEACAVVESVKAASDVYSPVTGEVVAGNAALGGQPELINSDPYGDGWI
                     MRVRPDDKAQFASMLDAKAYQAALAAETH"
     gene            complement(2029..3123)
                     /gene="gcvT"
                     /locus_tag="VD737_07940"
     CDS             complement(2029..3123)
                     /gene="gcvT"
                     /locus_tag="VD737_07940"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HXW10528.1"
                     /translation="MGLRTPLYDAHLAAGARTVDFGGWDMPVNYGSQIEEHHAVRRDA
                     GMFDVSHMCIVDLRGARVRDFLRRLLANDVAKLTQPGKALYTCMLRDDGGVIDDLIVY
                     FLEPEWYRMVVNAGTRDKDVAWLREHASGFGVAVEPRTDLAMIAVQGPNARQKAAQVL
                     DPATAERALQLGPFFGLDTGTLFVARTGYTGEDGWEIAMPAGEATAYWQKLVAAGVRP
                     CGLGARDTLRLEAGMNLYGNDMDETTSPLESGLGWTIAWEPQDRDFIGRGALAAQKAA
                     GDVRRFVGLLLEDRAVLRQHQKVIVANVGEGEVTSGTFSPTLQRSIGFARVPAQTADR
                     CEVEIRGKLVPARVVRPPFVRKGQVAVALD"
     regulatory      complement(3300..3387)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3616..>4363
                     /locus_tag="VD737_07945"
     CDS             3616..>4363
                     /locus_tag="VD737_07945"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HXW10529.1"
                     /translation="MKLLVGLIIALPLAAQAAPETQRSTAAVELHRLFDAEWERNLRE
                     NPISATYIGETRYNDRWPDLSLAGVQASHDADRAALARLEAIDPAALSDADRLNRDLF
                     RRQTRASLASFEYGEHMVPLSHRSGLTSAHRLADVIVFRSVRDYEQWLARMNGLDAPL
                     DQVLDLMREGVRRGLVPPKVLMQRLPKQIDAQIVSDPTQSPFYQPFAQMPATIPTADR
                     ERLQQAARTAITNTIVPAYTRLKQYLEQEYL"
BASE COUNT          636 a         1490 c         1535 g          702 t
ORIGIN      
        1 gtgcggccgg gcgtggagtg ttcgaaaatc agtttttcgt gcatctgggc ttcctcgcgg
       61 cgccgccggc ctcaggccgc ggcggccttg ctgcgcatcg tctcggcgag cgcctcggca
      121 taggtctcga cgtcgccgac cgtgcgcgtc tcggtcgcgc agacgagcag cgcgttgccg
      181 agttgcggat agcgcgccga aatgtcgacg ccgccgacga tgccgcgtcg cgccagcgcc
      241 tcgagcacgg gcctgaccgg ccggtcgagc agcagcactg cctcgtggaa gcgcggatgg
      301 tcgaaggcga gccgtacgcc cgggatgcgc gtcagcgcgt ccacgagctg ctgcgtgcgc
      361 tggatgcagg tggcggcgac ctgcgcgaga ccctgcgggc cgagcagcga catgtaaatc
      421 gtcgccgcgg tcaccagcag gccctggttc gtgcagatgt tcgaggtcgc cttgctgcgg
      481 cggatgtgct gctcgcgcgc ctgcagcgtg agcgtgaagc cggtcctgcc gtcgaggtcc
      541 agcgtgcgac cgatgatgcg gccgggcatc tggcgcacgt gttcggccct ggcggccatg
      601 aaaccgaagt acggcccacc cgaggacagc ggcacgccga gcggctggcc ttcgccgcag
      661 gcgatgtcgg cgccgcgggc gccccattcg ccgggcggct tcagcaccgc gagcgaggtc
      721 gggttcacga cgccgatcgc gagcgcgccg ttttcgtgcg cccagttcgt caccgcgtcg
      781 acgtcctcga gcgtgccgaa gaagttcggc tgctgcacca cgaccgcggt gatgtcctgg
      841 ccgcggtact ggtcgagcgc ggcgagcacg gtctggccgc tcctggcgtc gaacggcacc
      901 gactcgaacg cgaggttctg gttgccgcag atcgcctgcg cgacgtcccg gtaggtcggg
      961 ttgaccgccg acggcaccag gatgcgctgc gacttcgacc gccggttggc gcgcaccgcc
     1021 atcaggcacg cctccgcgag cccggaagcg ccgtcgtaga gtgaagcgtt cgagacgtcg
     1081 agcccggtca ggctcgccat catcgtctgg tattcgtaga gcagctgcag cgtgccctgg
     1141 ctcgcttccg cctggtaggg cgtgtacgcg ctgtagaact cgccgcgcgt cacgatggcc
     1201 cagacggccg cgggaatgtg gtgctcgtag gcacccgcgc cgatgaagtt gagcggccgg
     1261 ccgtcgcgcg cggccagctc cgccatcagg cggccgacct ccatctcggt catggccggc
     1321 gggatgccgg gaagacccgg cgagcgcagc tcggccggga tctcctcgaa cagcgcgtcg
     1381 atgtcgggcg cgccgatcgc gtcgagcatg gcgcggacgt cggcgtcggt gtgggggatg
     1441 aatggcatgg gagtggccag tgggtcgatc gtggggaagt gcggttagcg gttagcggtt
     1501 agcggttagt cggaaatgga tcggcggcgc agccgccttc ccactaacca ctaaccgcta
     1561 accgctgttc acttcttcag tgtgtttccg ccgccagtgc ggcctggtat gccttcgcgt
     1621 cgagcatcga cgcgaactgc gccttgtcgt cggggcgcac gcgcatgatc cagccgtcgc
     1681 cgtagggatc ggaattgatc agttccggct gcccgccgag ggccgcgttg ccggccacca
     1741 cctcgccggt cacggggctg tacacgtccg aggccgcctt gacggattcg actacggcgc
     1801 acgcctcgcc agcgttgacg cgacggccgg gctcgggcgc ctcgacgaac acgaggtcac
     1861 cgagcgcctg ctgcgcgtgg tccgagatgc cgatcgtgat cgtgccgtcg ttttcgaggc
     1921 gcgcccactc gtgcgacttg aggtagcgga ggtcggcggg aacgttgctc atggtgttct
     1981 ccgggagagg actcgtgttc tgcgatgctt tgtcggacgt gctcttgatc agtcgagcgc
     2041 cacggcgacc tggcccttgc gcacgaacgg cggccggacc acccgcgcgg gcaccagctt
     2101 gccgcggatc tccacctcgc agcggtccgc ggtctgcgcg ggcacgcgcg cgaagccgat
     2161 cgagcgctgc agcgtcggcg agaacgtgcc gctcgtgact tcgccctcgc ccacgttggc
     2221 gacgatgact ttctggtgct ggcgcagcac ggcgcggtcc tcgagcagca gcccgacgaa
     2281 cctgcgcacg tcgccggctg ccttctgcgc ggcgagcgcg ccacggccga tgaagtcgcg
     2341 atcctgcggc tcccaggcga tcgtccagcc gaggcccgat tcgagcggcg aggtggtctc
     2401 gtccatgtcg ttgccgtaca ggttcatgcc ggcctcgaga cgcagcgtgt cgcgcgcgcc
     2461 gagaccgcac ggccgcacgc cggccgcgac gagtttctgc cagtacgcgg tggcctcgcc
     2521 tgccggcatc gcgatctccc atccgtcctc gccggtgtac ccggtgcgcg cgacgaacag
     2581 cgtgcccgtg tcgagcccga agaacggccc gagctgcagt gcgcgctcgg ctgtcgcggg
     2641 gtccaggacc tgcgcggcct tctggcgcgc gttcgggccc tgcacggcga tcatcgcgag
     2701 atccgtgcgc ggttcgaccg cgacgccgaa cccggacgcg tgttcccgga gccaggcgac
     2761 gtccttgtcg cgggtgcccg cgttgacgac catccggtac cactcgggct cgaggaaata
     2821 gacgatcagg tcgtcgatga cgccgccgtc gtcgcgcagc atgcaggtgt agagcgcctt
     2881 gccgggctgc gtgagcttgg cgacgtcgtt ggcgagcagc cgtcgcagga agtcacggac
     2941 gcgcgcgccg cgcaggtcga cgatgcacat gtgcgagacg tcgaacatgc cggcgtcgcg
     3001 gcgcacggcg tggtgctcct cgatctgcga gccgtagttg accggcatgt cccagccgcc
     3061 gaaatcgacg gtgcgggcgc cggccgcgag gtgcgcgtcg tagagcggag tgcgtagtcc
     3121 catcgtccgt cctcgggcag tcgccactgc ggttggccaa agaaaaaggg cggcagaccg
     3181 cctgcgcgac ctgccgcccc tctgtccttg gacctgagag atcgagcacc gggctcgagt
     3241 cccgacgccg caccccttcg gtgggcccgc ctggcgacgg accgctctcc agagaccatc
     3301 aggccgcagc cgtacgtttg cctgagcgtt tccgggcggg ttgcgccttc ggcgggctcc
     3361 gtggagcctc tctcgggacg ggcctagcga tgggtgggcg catggtaccc caccccggaa
     3421 tagagggaca ctccgaattt ccgggataat ggggacactc ctgaattacg atcccgcggc
     3481 cgcggcaggc cactcgatca cggtcgctgc cggcgacgtc gtgcgaatct gccggcaatc
     3541 aggctgtgga aattgggagt gtccccaatt cgtgggaaat tcggagtgtc cccatttaat
     3601 ggagtgtcac agttcatgaa gctgctggtc gggttgatca tcgcgctgcc gctggcggcg
     3661 caggccgctc cggagacgca gcgctccacc gccgccgtcg aactgcaccg gctgttcgat
     3721 gccgagtggg agcgcaacct gcgcgagaac ccgatcagcg cgacctacat cggtgagacg
     3781 cgctacaacg atcgctggcc cgacctgtcg ctggccggcg tgcaggcctc gcacgacgcc
     3841 gaccgggcag ccctcgcgcg gctcgaggcg atcgacccgg cggcgctgag cgacgccgat
     3901 cggctgaacc gcgacctgtt ccggcgacag acccgggcga gcctcgccag cttcgagtac
     3961 ggcgagcaca tggtcccgct gtcgcaccgc agtggcctga cgagcgcgca ccggctcgcg
     4021 gacgtcatcg tcttccgctc cgtcagggac tacgagcagt ggctcgcgcg catgaacggc
     4081 ctcgacgcgc cactggacca ggtcttggac ctgatgcgcg agggcgtccg ccgcggtctc
     4141 gtgccgccga aggtgctgat gcagcgcctg ccgaagcaga tcgacgcgca gatcgtctcg
     4201 gacccgacgc agagcccgtt ctaccagcct tttgcgcaga tgcccgccac gatcccgacc
     4261 gccgatcgcg agcgtctgca gcaggcggcg cgcaccgcga tcacgaacac gatcgtgccc
     4321 gcctataccc gcctgaagca gtacctcgag caggaatacc tgc
//
LOCUS       DASPFR010000303         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1795077, whole genome shotgun sequence.
ACCESSION   DASPFR010000303 DASPFR010000000
VERSION     DASPFR010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1795077"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /locus_tag="VD737_07950"
     CDS             complement(<1..1425)
                     /locus_tag="VD737_07950"
                     /inference="COORDINATES: protein motif:HMM:NF023683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10530.1"
                     /translation="MKPAVAAALVTVALGGCSASKDGDPYPLPLTDVFKAEFNTTSGS
                     LPYPTDLFFNGSTDGTLNPPATIPWRPASNREALSALDGWSTTAPITTRFSMAIDPAS
                     LGAGSVRVVELYLSNTTKAPASGAELPQGVPSPVRRVLTPDVDYVVGVGDEVDGNGRT
                     LKITPLKPLTPSTGATNIGYLVLLTNGIDGADGQAAIPDDQYASYLSAPADCSSFPTT
                     DALNFGLCRLTKAHLQIAAGVGLDPASVVMSWTFSTQSIEDTLDVLSAITTAQPITVV
                     PSGLDTKQASGGLLQGKANVYVGRTVVPYYLTPAANPTDRASVLTKFWVAAGPPAVPG
                     LDPASRNLTRFNPVPEKKADVAIPLLVTVPNATAAGGACVKPASGWPVAIVQHGITGN
                     RTQALAVADSFADACVVVAAIDLPLHGLVPDQDGVDPNTTSPLFCSSTTPNPACMGAI
                     ERTFNVDLINNTTSAATSDNLIDSS"
     gene            1524..>2139
                     /gene="rsmD"
                     /locus_tag="VD737_07955"
     CDS             1524..>2139
                     /gene="rsmD"
                     /locus_tag="VD737_07955"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HXW10531.1"
                     /translation="MPRNNRKDARRSGGFAAATKPPIKRPKPAAGRASGAGRGEIRII
                     GGRWRGRKLHFPNVPGLRPTPDRVRETLFNWLQFRIAGTRCLDLFAGSGALGLEALSR
                     GAAEVLFVERDPQAADAIREALSLLGGPGLVDRRDAFAFLSEAAPRPFDVVFLDPPYD
                     RHWLPRLCAALESGGWLEPDARIYLEDAAERGETTLPVGWTLLRS"
BASE COUNT          297 a          717 c          777 g          348 t
ORIGIN      
        1 ggaggagtcg atcaggttgt cgctggtcgc cgcgctggtc gtgttgttga tcaggtcgac
       61 gttgaacgtg cgctcgatcg cgcccatgca cgccgggttc ggcgtcgtcg acgagcagaa
      121 gagcgggctc gtcgtgttcg ggtccacgcc gtcctggtcg gggaccagcc cgtgcagcgg
      181 caggtcgatc gccgcgacca cgacgcacgc gtccgcgaac gagtccgcga ccgccaatgc
      241 ctgcgtacgg ttgcccgtga tgccgtgctg cacgatcgcc accggccagc ccgacgcggg
      301 cttgacgcag gcaccgcccg ccgcggtggc gttggggacg gtcacgagca gcggaatcgc
      361 cacgtcggcc ttcttttccg gaaccgggtt gaagcgcgtc aggttgcggc tcgccgggtc
      421 gaggccgggg accgcaggcg ggcccgccgc cacccagaat ttcgtcaaca cggacgcgcg
      481 atccgtcggg ttggccgccg gcgtcaggta gtacggcacc acggtgcgcc cgacgtagac
      541 gttggccttg ccttgcagca gtccacccga cgcctgcttc gtgtcgaggc ccgaggggac
      601 gaccgtgatc ggctgcgccg tcgtgatcgc gctcagcacg tcgagcgtgt cctcgatcga
      661 ctgcgtcgag aacgtccagc tcatcacgac gctggccggg tcgaggccga cgccggccgc
      721 gatctgcagg tgcgccttgg tcagccggca cagcccgaag ttgagcgcgt cggtcgtcgg
      781 aaaactgctg cagtcggccg gcgcgctcag gtacgaagcg tactggtcgt cagggatcgc
      841 ggcctggccg tcggcgccgt cgatgccgtt ggtcaacagc acgaggtagc cgatgttggt
      901 cgcgccggtg ctcggcgtga gcggcttgag tggcgtgatc ttgagcgtgc ggccgttgcc
      961 gtcgacctcg tcgccgactc cgacgacgta gtcgacgtcg ggcgtcagca cgcgacgcac
     1021 cgggctcggc acgccttggg gcagttcagc gccgctcgcg ggggccttgg tcgtgttgct
     1081 gaggtacagc tcgaccacgc gcacggagcc ggcgcccagc gaggccgggt cgatcgccat
     1141 gctgaagcgg gtcgtgatcg gcgcggtggt cgaccagccg tcgagcgcgc tcagcgcctc
     1201 tcgattcgag gcagggcgcc acggaatcgt ggccggcggg ttcagcgtgc cgtcggtcga
     1261 gccgttgaag aacaggtcgg tcgggtaggg cagcgagcct gaggtcgtgt tgaactcggc
     1321 cttgaagacg tccgtcagcg gcagcgggta cggatcgccg tccttggacg ccgagcaacc
     1381 ccccagcgcg acggtcacga gcgcagccgc aaccgccggc ttcatcagcc tgttcctgga
     1441 ctcgaacatg gaattcccct cgttgattat ccgcgcgtac catcgcgcgt cgtgatcgtt
     1501 tcgaagccta cgctcgcccg ttgatgccgc gcaacaaccg caaggatgcc cggcgcagcg
     1561 gtggttttgc agccgcaaca aagcccccga tcaagcgccc gaaacctgcc gccggccgag
     1621 cttcgggcgc gggccgaggc gagatcagga tcatcggcgg ccgatggcgt ggacgtaagc
     1681 tgcactttcc gaacgtgccg ggcttgcggc cgaccccgga ccgggtccgc gagacgctgt
     1741 tcaactggct gcagttccgg atcgcgggga cgcgctgcct cgacctgttc gcgggcagcg
     1801 gcgcactggg gctggaggcc ctctcgcggg gcgctgcgga ggttctgttc gtcgagcggg
     1861 atccgcaggc ggccgacgcg atccgggagg cgctgtcgct gctcggtggc ccgggcctgg
     1921 tcgaccgcag ggacgccttt gcgttcctgt cggaggcggc cccgcggccg ttcgacgtcg
     1981 tcttcctgga tccgccctac gaccgccact ggctgccccg gctctgcgcg gccctggaat
     2041 ccgggggctg gctcgagccg gacgcccgga tctatctcga agacgcggct gaacgcggcg
     2101 aaaccaccct gccggtcggg tggacgctgc tgcgcagcg
//
LOCUS       DASPFR010000304         2778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1674571, whole genome shotgun sequence.
ACCESSION   DASPFR010000304 DASPFR010000000
VERSION     DASPFR010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674571"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VD737_07960"
     CDS             complement(<1..477)
                     /locus_tag="VD737_07960"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005803358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HXW10532.1"
                     /translation="MTTKSERNERPGDSVKRGAATRGQAPWIAPSILSADFARLGEEV
                     RNVLAAGADVVHFDVMDNHYVPNLTIGPLVCEALRKHGITAPIDVHLMVKPVDRIVPD
                     FARAGATYITFHPEATEHVDRTIGLIREHGCRPGLVFNPATPLVWLDHVLDKLDMVL"
     gene            complement(512..1366)
                     /gene="djlA"
                     /locus_tag="VD737_07965"
     CDS             complement(512..1366)
                     /gene="djlA"
                     /locus_tag="VD737_07965"
                     /inference="COORDINATES: protein motif:HMM:NF006948.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HXW10533.1"
                     /translation="MEGWYGKIIGAILGYIVGRGLLGAIVGFVIGHQFDVIARRNRGG
                     ALGGRRPAAIGAGHGSPAEMRQAFFEATFQVMGHVAKSDGRVSEREIDAARVAMHRFS
                     LSEADRVRAIELFTAGKNPGFPLEATLQRLHWLMGNQSNLARLFVQIQLEAAIDGDGL
                     GDRTRGVFVRICQSLGISAFEFASLEAMLRMHRSSRQRQSNGGQGGGASATTASRLAD
                     AYDALGVKATASDAEVRRAFRKLVSKNHPDKLVAQGLPESMITAAHERTQRILEAYET
                     IKNHRGFR"
     gene            1410..2414
                     /locus_tag="VD737_07970"
     CDS             1410..2414
                     /locus_tag="VD737_07970"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HXW10534.1"
                     /translation="MTVHLISPHGRAMSTPTSADSTTAARSVSATATASPPMHESHLP
                     GLTRLHQGKVRDIYEIPGDPRHMLIVTTDRLSAFDVVLPDPIPGKGRVLTSISNFWFE
                     RTGHILPNHLAEPARRHLDDIVPDAGDRASIADRAMVVRRLKALPVEAVVRGYLIGSG
                     WKDYQQSGEVCGIRLPAGLAMADKLPQPIFTPATKAPKGHHDENVGFGEIVKLVGEDL
                     ATQVRQTAIALYEFAARHALERGIIIADTKFEFGVDEEQGGRLTLIDEALTPDSSRFW
                     PVDTYAPGTSPPSFDKQFVRDYLETLDWNKTPPAPSLPADVIRRTSEKYEEALRRLTA
                     "
     gene            complement(2404..>2778)
                     /locus_tag="VD737_07975"
     CDS             complement(2404..>2778)
                     /locus_tag="VD737_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10535.1"
                     /translation="MRVVRNAAAEGSFVSLPELSLVIDGPEGQLVVVGCSHPGIETIL
                     RSTGAPRRPVDLVVGGLHLVTANDAQIERVLGVLRTDMQVQRMAPAHCTGEPAFAALL
                     RAYGDQYLYAGAGSVIDLPVRP"
BASE COUNT          437 a          996 c          921 g          424 t
ORIGIN      
        1 cagcaccatg tcgagcttgt cgagcacgtg gtcgagccac accagtggcg tcgccgggtt
       61 gaacacgaga cccggcctgc agccgtgctc gcggatcagc ccgatggtgc ggtcgacgtg
      121 ctcggtcgcc tcggggtgga acgtgatgta ggtggcacca gccctggcga agtccggcac
      181 gatgcggtcg acgggcttga ccatcaggtg cacgtcgatc ggcgccgtga tgccgtgctt
      241 gcgcagcgcc tcgcagacca gcggcccgat cgtgaggttc ggtacgtagt ggttgtccat
      301 cacgtcgaag tgcaccacgt ccgcgccggc cgcgagcacg ttgcggactt cctcgccgag
      361 ccgcgcgaaa tccgcggaca ggatcgacgg cgcaatccac ggcgcctgcc cgcgggtcgc
      421 agcgccccgc ttcacagagt ccccgggtcg ctcgttccgc tcggatttcg tcgtcatggc
      481 acgatactcg cttgggacgg gctgctggcc gtcaccggaa gccgcgatgg ttcttgatgg
      541 tctcgtaggc ctcgaggatc cgctgcgtgc gctcgtgcgc cgccgtgatc atcgactccg
      601 gcaatccctg cgcgaccagc ttgtcggggt ggttcttgct gacgagcttg cggaacgcgc
      661 gacgcacctc ggcgtcgctc gccgtcgcct tgacgccgag cgcatcgtac gcgtcggcga
      721 gccgcgacgc ggtcgtggca ctcgcgccgc cgccctggcc gccgttcgac tgtcgctggc
      781 ggctgctgcg gtgcatgcgc agcatcgcct cgagcgaggc gaactcgaac gcggagatgc
      841 cgagcgactg gcagatgcgc acgaacactc cccgcgtgcg gtcgccgagt ccgtcgccgt
      901 cgatcgccgc ctcgagctgg atctgcacga acaggcgcgc caggttggac tggttgccca
      961 tcagccagtg caggcgctgc agcgtcgcct cgagcgggaa gccgggattc ttgccggccg
     1021 tgaacagttc gatcgcgcgg acccggtcgg cctcgctcaa cgagaagcgg tgcatcgcga
     1081 cgcgcgccgc gtcgatttcg cgctcggaga cccggccgtc gctctttgca acgtgcccca
     1141 tgacctggaa cgtcgcctcg aagaacgcct ggcgcatctc ggcaggactg ccgtggcccg
     1201 cgccgatcgc agcgggcctg cggccgccga gcgcgccgcc tcggttgcgc cgggcgatga
     1261 cgtcgaactg atgcccgatc acgaaaccga cgatcgcgcc gagcaggccg cgaccgacga
     1321 tgtagcccag gatcgcgccg atgatcttgc cgtaccagcc ttccaacttg ccttccgcgc
     1381 gaaaccacgc gtcctgagga gccgttacaa tgaccgtgca tcttatcagc ccccacggtc
     1441 gcgccatgtc cacccccacc tccgcagatt ccacgacggc cgcccgctcc gtgtcggcca
     1501 ccgccaccgc gtcgcccccg atgcacgagt cgcacctgcc ggggctcacg cggctgcacc
     1561 agggcaaggt ccgcgacatc tacgaaatcc ccggcgaccc gcgccacatg ctgatcgtga
     1621 ccaccgaccg cctgagcgcg ttcgacgtcg tgctgccgga cccgatcccg ggcaagggcc
     1681 gcgtgctgac gagcatctcg aacttctggt tcgagcgcac cgggcacatc ctgccgaatc
     1741 acctcgccga gccggcgcgc cgccacctcg atgacatcgt gcccgacgca ggcgaccgtg
     1801 cgtcgatcgc cgatcgtgcg atggtggtgc gacgtctgaa ggcgctgccg gtcgaagccg
     1861 tcgtgcgcgg ctacctgatc gggtcgggct ggaaggatta ccagcagagc ggcgaagtct
     1921 gcggcatccg cctgcccgcc ggcctggcga tggcggacaa gctgccgcag ccgatcttca
     1981 cgcccgcgac caaggctccc aagggccatc acgacgagaa cgtgggcttc ggcgagatcg
     2041 tcaagctggt cggcgaggat ctcgcgaccc aggtgcgcca gaccgcgatc gcgctgtacg
     2101 agttcgcggc gcgccacgcg ctcgagcgcg gcatcatcat cgccgacacc aagttcgagt
     2161 tcggcgtcga cgaggagcaa ggcggtcggc tcacgctgat cgacgaggcg ctgacccccg
     2221 actcgtcgcg cttctggcct gtcgatacct acgcgcccgg cacgagcccg ccgtcgttcg
     2281 acaagcagtt cgtgcgcgac tacctcgaga cgctcgactg gaacaagacg ccacccgcgc
     2341 cgtcgctgcc cgcggacgtg atccggcgca cgagcgaaaa gtacgaggaa gcgctgcgtc
     2401 ggctcacggc ctgaccggca ggtcgatcac gcttcccgcc ccggcataaa ggtactggtc
     2461 cccgtacgcg cgcaggagcg cggcgaacgc gggctcgccg gtgcagtggg ccggcgccat
     2521 gcgctgcacc tgcatgtcgg tgcgcagcac gccgagcacg cgctcgatct gcgcatcgtt
     2581 cgccgtgacg agatgaaggc cgcctaccac caggtcaacc gggcggcgcg gcgcgccggt
     2641 ggatcgcagg atcgtttcga tgccgggatg cgaacagccg acgaccacca gctgtccttc
     2701 cggcccgtcg atgaccaacg agagctcagg caggctgacg aaactccctt cggcggcggc
     2761 gttccgcacg acacgcat
//
LOCUS       DASPFR010000305         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_574984, whole genome shotgun sequence.
ACCESSION   DASPFR010000305 DASPFR010000000
VERSION     DASPFR010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574984"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..102
                     /locus_tag="VD737_07980"
     CDS             <1..102
                     /locus_tag="VD737_07980"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298007.1"
                     /note="catalyzes the formation of dUMP from dUTP; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HXW10536.1"
                     /translation="MVIVPVVQVEFEVVEEFDESHRGAGGFGSSGRK"
     gene            complement(205..897)
                     /locus_tag="VD737_07985"
     CDS             complement(205..897)
                     /locus_tag="VD737_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10537.1"
                     /translation="MTELTPLDTARRRAHLLNAGVAVVLLAALAAGVADAAVKKNRGG
                     PTLSNEEMYRCKAGNGRTYVSQAIPPECMDLDVEILDGTGRVVRVIPGRQTLEEIAQQ
                     KAAEDAKAAAAQRDKTLLATYLTVADIERLRDQRIELLEQQNVVTRQYISNLRAREAR
                     LMQSVQRYRPYSTKPNAPALPEQIASEIVNTVKGLQVYEQELTKNTSERERLTREFGA
                     DIQRFKELKGLK"
     gene            complement(974..1621)
                     /gene="pyrE"
                     /locus_tag="VD737_07990"
     CDS             complement(974..1621)
                     /gene="pyrE"
                     /locus_tag="VD737_07990"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HXW10538.1"
                     /translation="MHQRDFIKLCLELGVLRFGEFTLKSGRRSPYFFNAGLFNTGRAI
                     GALGRHYARAAATSGVAFDMLFGPAYKGIPLVTATAAAMAEHEGRDLPYAFNRKEAKD
                     HGEGGMIVGAPLRGRVLIVDDVITAGTAIRESVEVIRAQGAAPAGVLIALDREERGQG
                     EKSAVQEVEDQFGLPVVAVLRLSDLLAHLESAGDRAVLQTVRAYRDQYGVAGAVG"
     gene            1650..>2310
                     /locus_tag="VD737_07995"
     CDS             1650..>2310
                     /locus_tag="VD737_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HXW10539.1"
                     /translation="MRIITLNANGIRSAARKGFFDWLERQDADVVCVQETKCQREQIT
                     DTTFHPAGFHCHYFDATKKGYSGVALYSRARPDDVVVGYGSREFDGEGRYLEARFGKL
                     SVVSVYLPSGSSSEDRQRAKFRFLGEFMPYLRALKRKRRDYVLCGDWNIAHKEIDLRN
                     WKSNQKNSGFLPEERAWLTRVFEEVGWVDVFRRVDPRPERYTWWSNRGQAYAKNVGWR
                     ID"
BASE COUNT          357 a          775 c          779 g          399 t
ORIGIN      
        1 atggtgatcg tgccggtcgt gcaggtggaa ttcgaagtcg tcgaggagtt cgacgagagc
       61 caccgcgggg cgggcgggtt cgggagcagc ggccggaaat agtgtctagt gtctagtgtc
      121 cagtgttcag taggaggcgg cgacaccgcg cgccgacgca gtcggcttcc tactaaccgc
      181 tagacactag acactgaaca cttcctactt cagtcccttc agctccttga accgctggat
      241 gtccgcgccg aattcgcgcg tcaggcgctc gcgctcgctg gtgttcttgg tgagctcctg
      301 ctcgtagacc tgcaggccct tgaccgtgtt gacgatctcg ctcgcgatct gctccggcag
      361 cgccggagcg ttgggcttgg tgctgtaggg acgatagcgc tgcaccgact gcatcagccg
      421 cgcctcgcgt gcacgcaggt tcgagatgta ttgccgcgtc acgacgttct gctgctcgag
      481 cagctcgatg cgctggtcgc gcagcctttc gatgtccgcc acagtgaggt aggtcgcgag
      541 cagcgtcttg tcgcgctgcg cggcggccgc tttcgcgtcc tcggcggcct tctgctgcgc
      601 gatctcctcg agcgtctgcc gtcccgggat cacccgcacg acgcggccgg tgccgtcgag
      661 gatctcgacg tcgaggtcca tgcactcggg cggaatggcc tggctcacgt aggtgcggcc
      721 gttgccggcc ttgcaccggt acatctcctc gttcgagagc gtcggtccac cgcgattctt
      781 cttcacggcg gcgtcggcca cgccggccgc gagggccgcc agcagcacta cggccacgcc
      841 cgcgttcagc aggtgggcgc gcctgcgagc cgtatcgaga ggagtcagct cggtcatgcc
      901 ggacattctg aggcagccgc cctccggatt tgcacctttt gaccccgtgc agccttcggc
      961 actgtgaccg acttcacccg acggcgcccg cgacgccgta ctggtcgcgg taagcccgca
     1021 cggtctggag cacggcacgg tcgccggcgg actcgaggtg cgcgagcagg tcgctgaggc
     1081 gcaggaccgc cacgacgggc agcccgaact ggtcctcgac ctcctggacg gccgacttct
     1141 cgccctggcc acgctcctcg cggtcgagcg cgatcagcac gccggccggc gcggccccct
     1201 gggcgcggat gacctcgacc gactcgcgga tcgcggtgcc ggcggtgatc acgtcgtcga
     1261 cgatcaggac gcggccccgc aatggcgcgc ccacgatcat gccgccttcg ccatggtcct
     1321 tggcttcctt gcggttgaac gcataaggca ggtcgcggcc ctcgtgctcg gccatcgcgg
     1381 ccgccgtcgc ggtgacgagc gggatgccct tgtaggccgg cccgaacagc atgtcgaacg
     1441 cgacgccgga cgtcgctgcc gcgcgcgcat agtgccggcc gagcgcgccg atggcgcggc
     1501 ccgtgttgaa gagaccggcg ttgaagaaat acgggctgcg gcggccggac ttcagcgtga
     1561 actcgccgaa ccgcagcacg ccgagctcga ggcaaagctt gatgaagtcg cgttggtgca
     1621 taagcctcgt actttatact gcgtgcccta tgcgcatcat cacgctcaac gcgaacggca
     1681 tccgttccgc cgcgcgcaag ggcttcttcg actggctcga acgacaggac gcggacgtcg
     1741 tctgcgtgca ggaaaccaag tgccagcgcg agcagatcac cgacacgacg ttccatccgg
     1801 cgggcttcca ctgccactac ttcgacgcga ccaagaaagg ctacagcggc gtcgcgctct
     1861 attcgcgcgc gcgccccgac gacgtggtag tgggttacgg ctcgagggag ttcgacggcg
     1921 agggccgcta cctcgaggcg cggttcggca agctgagcgt cgtgtccgtc tacctgccgt
     1981 ccggctcgtc gagcgaggac cggcagcgcg ccaagttccg cttcctgggc gaattcatgc
     2041 cgtacctgcg cgcgctcaag cgcaagcggc gtgattacgt cctctgcggc gactggaaca
     2101 tcgcgcacaa ggagatcgat ctgcggaact ggaagtcgaa ccagaagaac tccggcttcc
     2161 tgcccgagga gcgcgcctgg ctcacgcgcg tcttcgagga ggtggggtgg gtggacgtgt
     2221 tccgccgcgt cgacccgcgc cccgagcgct acacgtggtg gagcaaccgc ggccaggcgt
     2281 acgcgaagaa cgtcgggtgg cgcatcgact
//
LOCUS       DASPFR010000306         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_209415, whole genome shotgun sequence.
ACCESSION   DASPFR010000306 DASPFR010000000
VERSION     DASPFR010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209415"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VD737_08000"
     CDS             complement(<1..438)
                     /locus_tag="VD737_08000"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXW10540.1"
                     /translation="MMAAVLTEIGKPAPYASSRPLALEHVELADPGPGEVLVQIRAAG
                     LCHSDLSVIDGNRPRPMPMVLGHEMAGVVESVGGDVNDLRPGDHVVAAFVPSCGHCAP
                     CTGGRPALCEPGLAANTAGVLLSGERRLAQQGAQVNHHLGVSGF"
     gene            559..840
                     /locus_tag="VD737_08005"
     CDS             559..840
                     /locus_tag="VD737_08005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXW10541.1"
                     /translation="MEVTREDHGSKGRWHTVVEGHQAEMSYSRASPTLIIIDHTAVPD
                     ALRGRRVGQALVEQAVADARRDGFKILPLCPFAKSQFERHSDWSDVLSR"
     gene            921..1301
                     /locus_tag="VD737_08010"
     CDS             921..1301
                     /locus_tag="VD737_08010"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXW10542.1"
                     /translation="MDDDVYGHVNNVVYYSYFDTAVNGYLIAATGTDIRTLPAIGIVA
                     ETSCRFLQEIAFPDRVQPGLALERLGRSSVIYRIGLFRNDEESPCALGRFVHVYVDAT
                     TRRPVSVPELIRGVVSALPAAAES"
     gene            complement(1350..1958)
                     /locus_tag="VD737_08015"
     CDS             complement(1350..1958)
                     /locus_tag="VD737_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXW10543.1"
                     /translation="MLRLYDYLPSGNGYKVRLVLKLLALPYELVELDIKQGATRTPEF
                     LARNPNGRIPLLELNGGRYLAESHAIILYLAEGSALIPVELYERALVWQWLCFEQYNL
                     EPNVGTARYWLRSLGQSEAELGERLLEKRRNGAAALAVLERGLQQGEFLVGGRLTLAD
                     IALYAYTHVAPDGGFDLSRYPRVLAWCDRLTRIPGWAPITAR"
BASE COUNT          365 a          705 c          705 g          358 t
ORIGIN      
        1 aaaaccagac acgccgagat gatggttgac ctgggcgcct tgctgcgcga gacgacgttc
       61 gcccgacagc agcacaccag cagtgtttgc ggcgagccca ggctcgcaga gcgcgggtcg
      121 gccgcccgtg cacggggcgc agtgcccaca actcggcacg aacgccgcga cgacgtggtc
      181 gccgggccgc aggtcgttga cgtcgccacc gaccgactcg acgacaccgg ccatttcgtg
      241 tcccagcacc atcggcatgg ggcggggacg gttgccgtcg atcacggaca ggtcggaatg
      301 acacaggccg gccgcgcgga tctgcacgag cacctcgccg ggccccggat cggcgagctc
      361 gacgtgctcg agcgcgagcg gtcggctcga tgcgtacggt gcgggcttgc cgatctccgt
      421 cagtaccgcc gccatcattc gcatcgtcgt gtcccgagcg ccgtcgtggc tcgtgagcat
      481 agtgcgcacg cccgacgatg gcgatgccgg tgcgccctgt tagcatgccc accggcggcg
      541 gttgctcatg agggtgcgat ggaagtcacg cgggaagatc acggctcgaa aggccgctgg
      601 cacacagtcg tcgagggtca tcaggccgag atgtcctact cgcgagcatc gccgacgctg
      661 atcatcatcg atcatacggc cgtaccggat gcgctgcggg gccgtcgtgt cggacaggcg
      721 ctcgtcgaac aagccgtcgc cgatgcgagg cgcgacggtt tcaagatcct gccgttgtgc
      781 ccgtttgcga agtcacagtt cgagcggcac agcgactggt ccgacgtgct gtccagatga
      841 acgagcgagc gagtgctggg gagcttgcga ccctccgccg cgaacacttc cgtctcgtcg
      901 cgaccgtgtc gacccgctgg atggacgacg acgtgtacgg gcacgtgaac aacgtcgtct
      961 attactcgta tttcgacacc gcagtaaacg gctatctgat cgcagcgacc ggcaccgaca
     1021 tccgcactct gcccgcgatc gggatcgtcg cggaaacgag ctgtcgattt ctgcaggaga
     1081 tcgcgtttcc cgatcgcgtg cagccgggcc tcgcgctcga gcgcctcggc cgttccagcg
     1141 tgatctaccg gatcggtctg ttccgcaatg acgaggagtc gccatgcgcg ctcggccgct
     1201 tcgttcacgt gtacgtcgat gccacgacgc ggcgacccgt gtccgtcccc gaactgatcc
     1261 gcggtgtcgt cagcgcattg cccgccgccg ctgagtcctg accggtccag cgcgcacggg
     1321 cgttcgcggg cggcgtgagt ctgtgggaat cagcgcgccg tgatcggggc ccagccaggg
     1381 atccgggtga gacggtcgca ccacgcaaga acgcgcggat agcgagagag atcgaagccg
     1441 ccgtcaggcg cgacatgcgt gtaggcgtag agcgcaatgt cggccagcgt caatcggccg
     1501 cccacgagaa actcgccctg ttgcagccca cgctcgagaa ccgcgagcgc ggccgcgcca
     1561 ttgcgacgct tctcgaggag tcgctcgccg agttccgcct cgctttgccc gagcgagcgc
     1621 aaccagtagc gtgccgtacc cacgttgggc tcgaggttgt actgctcgaa acacagccat
     1681 tgccagacga gcgcgcgctc gtagagctcc acgggtatca gcgccgagcc ctcggccaga
     1741 tacagaatga ttgcgtggct ctcggcgaga tagcggccgc cgttgagctc gagcagcgga
     1801 atgcgaccgt tcggattcct ggccaggaat tcgggcgtgc gcgtcgcccc ttgcttgatg
     1861 tcgagctcga cgagctcata gggcagcgcc agcagcttca ggaccagccg caccttgtag
     1921 ccgtttcccg acggaaggta atcgtacagc cgcaacacgt ggtcatcctc atccatgcag
     1981 cgtggctgcg ccgcgggaga tcgcaagagc accgctgcgt acgacttcga gcacttcgcc
     2041 gtaagcgccg gccgtcgcga tgaagcggtt gatctgcgcc tcgctgtcgg tcagctccac
     2101 ggtgtagctc gccgtgtcat cgtgcagcac ctt
//
LOCUS       DASPFR010000307         5442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1309167, whole genome shotgun sequence.
ACCESSION   DASPFR010000307 DASPFR010000000
VERSION     DASPFR010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309167"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VD737_08020"
     CDS             <1..231
                     /locus_tag="VD737_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10544.1"
                     /translation="GDFNTFWGEHEMALFMEASGLRSANREGLPSYPARRPRLELDFI
                     LYTRGIEIDAFEVPAVTFSDHRPLLCDFHVTG"
     gene            259..2328
                     /locus_tag="VD737_08025"
     CDS             259..2328
                     /locus_tag="VD737_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXW10545.1"
                     /translation="MTASAQTTPAIDATASHWTVELDADRVAWLTFDKPGASANALSR
                     PAMSELNDRLAELERLRPAGVVIVSAKSGFIAGADITEFAQAPSPVETVPWVRLAHGV
                     MQRLEDLPFPSVAAINGYCLGGGLELALACRHRVCADDPKVTIGLPEVLLGIHPGFGG
                     TVRTVRVAGVTTAMDLMLTGRNYRPDKALKAGLVDAVVPAAQLREAAKRMALQPRPRA
                     RAPIAQRLLNLGPVRRVVAGKIEQQVARKARRDHYPAPYAIVDLWRRHGANPRTAYEA
                     EAQSVAQLVCTPTSRNLVRVFFLQERLKGLGGKELPKGGHVHVVGAGVMGGDIAAWCA
                     ARGFTVTLQDRAQEQVQPALERARAFFARRARKPGQAEEYMARLTADVEGLGVARAQV
                     VIEAIFENAHAKRDLYARLEPQLAADAVLATNTSSLMLEPLAEGLAQPGRLVGLHFFN
                     PVAQMPLVEVIESGNTDAGVRAAALAFTRAIDKLPLPCRSAPGFLVNRVLMPYMTEAM
                     LAAQEGVPLATIDEAAVAFGMPVGPIELADVVGLDVCLHVGRILSAAFGGPAPDAVAP
                     LVEAGKLGKKSGQGLYEWRDGKAVKPPVDRSAGVPDELTDRLMLVLVNEAMAVLREGI
                     VGDADLIDAGIIFGSGFAPFRGGPLKYARDRGVTDVVARLEALSQRHGARFRPDPGWQ
                     AFGAGTT"
     gene            2362..3444
                     /locus_tag="VD737_08030"
     CDS             2362..3444
                     /locus_tag="VD737_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXW10546.1"
                     /translation="MSTDSPLEPSFLRAFSPLDGLKNENLRALARKTTVRELTQGRLL
                     FKEGDTDKRTYYLATGLLELMSGGRVVGTLRGGTPDARHPIAPVLPRRCAARVASDRI
                     EYLSIDSDMLDVLLTWDQTGQYEVGELRSGDPSSDDWMTMLLQSRAFHRIPPANLQAV
                     FMRMQRMTYAAGDTVIRQGDEGDYFYVIVDGRCAVSRETPLNREGIRLAELGKGETFG
                     EEALISGARRNATVTMLTDGALMRLAKEDFNTLLEEPLLQWVDYERAQQIVAGGGAWL
                     DVRLPSEYEHWHLEPSINVPLYFVRLKIKTLDPTVPYVAVCDNGRRSSAAAYVLSERG
                     FEAYVLRGGIGDTELAEALIQTSGSG"
     gene            complement(3546..3908)
                     /locus_tag="VD737_08035"
     CDS             complement(3546..3908)
                     /locus_tag="VD737_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003179413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXW10547.1"
                     /translation="MAAPATRQPNKPGLLTLTIKDKSALYLAYMPFVKNGGLFIPTSS
                     NYRLGDEVFMLLNLMGEDEKLPVAGRVIWMTPKGAQGKRTAGIGVQFSEQDRGQTQRK
                     IESYLAGALGGDKPTHTM"
     gene            complement(3930..4961)
                     /gene="holB"
                     /locus_tag="VD737_08040"
     CDS             complement(3930..4961)
                     /gene="holB"
                     /locus_tag="VD737_08040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HXW10548.1"
                     /translation="MTTTDAEAAVEDALAPPPIHLLPWHAAACDRLSAAVVAGRLPHG
                     LLLQGPAGVGKERFASALAAALFCTGRGERLEACGTCAECRLSRAGTHPDVHWVLPIE
                     DRKTIGVDQVREVCEQLAMTSMRRGYRVAIVAPADRLTPQAQNALLKTLEEPAPRTVI
                     VLVTARPSALLPTLRSRCQRVEIARPDPQVAARWLSEALGRPPPLRLLAVAGGAPLKA
                     ASLAPHFEDLEQQMTGLLEAFLGGRIDVTRAAADMQGEGLPTRLDWVEGWLGSIVQRA
                     AGVVDETALTFRPGSPLQRATTELNITAAFQVLDRLREARRLLEGSASAPLVVETVLL
                     ELRAAVRPH"
     gene            complement(4958..>5442)
                     /gene="tmk"
                     /locus_tag="VD737_08045"
     CDS             complement(4958..>5442)
                     /gene="tmk"
                     /locus_tag="VD737_08045"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HXW10549.1"
                     /translation="RSDEPMPQACELLLMFAARASHLANLVEPAVARGAWVLCDRFTD
                     ATYAYQGGGRGVAEADIDALVRIVHPRRQPDLTLLLDAPVEVGMRRAHARNGSVGPDR
                     FESERSDFFERVRRCYLQRAAREPARFRVVDASRAPDDVEACVREALARLLEAAETGT
                     "
BASE COUNT          815 a         1919 c         1890 g          818 t
ORIGIN      
        1 ggcgacttca acacgttctg gggcgagcac gagatggcgc tgttcatgga ggcgagcggc
       61 ctgcgcagcg ccaaccgcga aggcctgccc tcgtatccgg cacgccgtcc ccggctcgag
      121 ctcgacttca tcctgtacac gcgcggcatc gagatcgacg ccttcgaggt gcccgccgtc
      181 acgttctcgg accatcgtcc gttgctctgc gacttccacg tcacgggctg atgccgacga
      241 cgtgcagagg atccttccat gaccgcctcc gcccagacca cgccggccat cgacgcgacg
      301 gcgtcgcact ggacggtcga gctcgacgcc gaccgcgtcg cctggctcac gttcgacaag
      361 cccggcgcca gcgccaacgc gttgtcgcgg ccggcgatgt cggagctgaa cgatcggctc
      421 gccgagctcg agcggctgcg gcccgctgga gtggtgatcg tctccgcgaa gagcgggttc
      481 atcgccggcg ccgacatcac tgagtttgca caggcgccct cgcctgtcga aaccgtgccg
      541 tgggtgcgcc tggcgcacgg ggtcatgcag cggctcgagg acctgccttt cccgagcgtc
      601 gcggcgatca acggctattg cctgggcggt gggctcgagc tcgcgctggc ctgccgtcac
      661 cgggtctgcg ccgacgatcc caaggtgacg atcggactgc cggaagtgct gctcggcatc
      721 catccgggat tcggcggcac cgtccggacg gtgcgcgtgg ccggcgtcac cacggcgatg
      781 gacctgatgc tgacgggcag gaattatcgg cctgacaagg cgctcaaggc cgggctcgtc
      841 gacgcagtcg tgccggccgc tcagctgcgc gaggctgcaa agcggatggc actgcagcca
      901 cgcccccgcg cacgcgcacc gatcgcgcag cggctcctca acctgggtcc cgtgcgccgc
      961 gtcgtcgcgg gcaagatcga gcagcaggtt gcgcgcaagg ctcgccgcga ccactatccc
     1021 gctccgtacg cgatcgtgga cctctggcgc cggcacggcg ccaaccctcg caccgcctac
     1081 gaggccgagg cccagtcggt cgcccagctg gtgtgcacgc caacctcgcg caacctcgtg
     1141 cgagtgttct ttttgcagga gcgcctcaag ggcctcggcg gcaaggagct gccgaaaggc
     1201 ggccacgtcc acgtcgtggg cgccggcgtc atgggcggcg acattgccgc ctggtgcgcg
     1261 gcccgcggct tcaccgtcac gctgcaggat cgggcgcagg agcaggtgca gcccgcgctc
     1321 gagcgggcac gcgcgttctt cgcccggcgc gcgagaaagc cggggcaggc agaggaatac
     1381 atggcgcgcc tcacggccga cgtcgaaggc ctgggcgtgg cgcgcgcgca ggtcgtgatc
     1441 gaggcgatct tcgagaacgc gcacgccaag cgcgatctct atgcgcgcct cgagccgcag
     1501 ctcgccgcgg acgcggtgct ggcgaccaac acgtcgagcc tgatgctcga gccgctcgcc
     1561 gaggggctgg cgcagccggg ccggctcgtc ggcctgcact tcttcaatcc cgtcgcgcag
     1621 atgcccctcg tcgaggtcat cgagtccggc aacaccgacg cgggggtccg cgcggccgcg
     1681 ctcgcgttca ctcgcgcgat cgacaagctg ccgctgccgt gtcgcagcgc tcccgggttc
     1741 ctcgtcaacc gggtgctgat gccgtacatg accgaggcga tgctcgccgc acaggagggc
     1801 gtgccgctcg ccacgatcga cgaggctgcg gtcgcgttcg gcatgccggt gggcccgatc
     1861 gagctggcag acgtcgtcgg gctggacgtc tgcctgcacg tcgggcggat cctctcggcg
     1921 gcgttcggcg gacccgcgcc cgacgccgtc gcgccgctgg tggaggccgg caagctcggc
     1981 aagaagagcg gccagggcct ctacgagtgg cgcgacggga aggccgtgaa gccaccggtc
     2041 gaccgctcgg ccggcgtgcc cgatgaactg accgaccggt tgatgctggt gctcgtcaac
     2101 gaggcgatgg cggtgctgcg cgaagggatc gtgggcgacg ccgacctgat cgatgcgggc
     2161 ataatcttcg gctcgggttt tgcgccgttc cgcggcgggc cgctcaagta cgcgcgcgac
     2221 cgcggcgtga ccgacgtcgt cgcccggctg gaggcgctgt cgcagcggca cggtgcacgg
     2281 ttccggccgg accctggctg gcaggcattc ggtgccggga ccacgtgata tcatgtcgaa
     2341 tcttttgcca tcgcgtcctg aatgagcacc gatagcccgc ttgaaccgag cttcctgcga
     2401 gccttttcgc cgctcgacgg gctcaagaac gagaacctgc gggcgctcgc gcgcaagacc
     2461 acggtgcgcg aactcaccca gggtcgcctg ctgttcaagg aaggcgacac cgacaagcgc
     2521 acgtattacc tggcgaccgg actgctcgag ttgatgtccg gcggtcgcgt ggtcggcacg
     2581 ctccggggcg gtacgccgga cgcacgccac ccgatcgcgc cggtgctgcc gcgccgctgc
     2641 gccgcgcgcg tcgcctccga tcgcatcgaa tacctgtcca tcgacagcga catgctcgac
     2701 gtgctgctga catgggacca gacaggccag tacgaagtcg gcgagctgcg cagtggcgac
     2761 ccctccagcg acgactggat gacgatgctg ctgcagagcc gcgcgttcca ccggatcccg
     2821 cccgcgaacc tgcaggccgt gttcatgcgc atgcagcgca tgacgtatgc cgccggcgac
     2881 accgtgatcc ggcagggcga cgaaggcgac tatttctacg tgatcgtcga cggccgttgc
     2941 gcggtcagtc gcgagacgcc gctcaatcgc gaaggcatcc ggctggccga actcggcaag
     3001 ggcgagacct tcggcgagga ggccctgatc tccggtgcgc ggcgcaacgc cacggtgacg
     3061 atgctcaccg acggcgcgct gatgcgcctc gccaaggaag acttcaatac gctgctcgaa
     3121 gagccgctgc tgcagtgggt ggactacgag cgggcgcagc agatcgtcgc gggcggcggc
     3181 gcgtggctcg acgtgcgcct gccgagcgag tacgagcact ggcacctcga gccgtcgatc
     3241 aacgtgccgc tctacttcgt gcggctcaag atcaagacgc tcgaccccac ggtgccgtac
     3301 gtcgccgtgt gcgacaacgg ccgccgcagc tccgcggcgg cctacgtcct gagcgagcgc
     3361 ggcttcgagg cgtacgtgct gcgcggcggc atcggcgaca ccgagctggc ggaagcgctg
     3421 atccagacca gcggaagcgg ttagcggtta gcggttagtg ggaaagcacg tcgtgcgcag
     3481 aaaaagcaga aggcgcccga gggcgccttc tgactaaccg ctgatcacta accgctaacc
     3541 gctgcctaca tcgtgtgcgt cggcttgtcc ccgcccagtg cgccggccag gtatgactcg
     3601 atcttgcgct gggtctggcc gcggtcctgt tcgctgaact gcacgccgat cccggcggtg
     3661 cgcttgccct gggcaccctt gggcgtcatc cagatcacgc gtccggccac cggcagcttc
     3721 tcgtcctcgc ccatcaggtt tagcagcatg aacacctcgt cgccgaggcg gtagttgctg
     3781 ctggtcggga tgaagagccc gccgttcttc acgaacggca tgtaggccag gtacagcgcg
     3841 ctcttgtcct tgatggtgag cgtcagcagt ccgggcttgt tcggctgtct cgtcgcgggc
     3901 gcggccatcc gtttacctgt ctggctccgt cagtgcggcc ggaccgctgc gcgcaactcg
     3961 agcagcacgg tctcgaccac caggggcgcg gaggccgagc cttccagcag gcggcgggct
     4021 tcgcgcaggc gatccagcac ctggaacgcc gcggttatgt tcaactccgt ggtagccctt
     4081 tgcaagggcg aaccgggccg aaacgtgagc gccgtctcat cgaccacgcc cgctgcacgc
     4141 tgaacgatgc tgccgagcca cccttcgacc cagtccaggc gcgtcggcag cccctcgccc
     4201 tgcatgtccg cagcggcccg ggtaacgtcg atgcgaccgc cgaggaatgc ctcgagcagc
     4261 ccagtcatct gctgctcgag atcctcgaaa tgtggcgcga ggctcgccgc cttgagcggt
     4321 gctccgcccg cgacggcaag cagccgcaac ggcggcggac gacccagcgc ctcgctgagc
     4381 cagcgcgcgg ccacctgggg atcgggcctc gcgatctcga cgcgctggca gcggctgcgc
     4441 agcgtcggca gcagcgccga cggtctcgcg gtcacgagca cgatcacggt gcgcggtgcc
     4501 ggctcctcga gcgtcttgag caacgcattc tgcgcctgcg gcgtcaggcg gtcggcgggc
     4561 gcgacgatcg cgacgcggta gccgcgtcgc atgctcgtca tcgcgagctg ctcgcacacc
     4621 tcgcgcacct ggtcgacgcc gatcgtcttc ctgtcctcga tcggaagcac ccagtgcacg
     4681 tccgggtgcg tgccggcccg gctcaaccgg cactcggcgc acgtgccaca ggcttcgaga
     4741 cgctcgccgc ggcccgtgca gaacagtgca gcggcgagcg ccgaggcaaa ccgctccttg
     4801 ccgacacccg cgggaccctg cagcaacagg ccgtgcggca ggcgtccggc aacgacggcc
     4861 gcactcagcc gatcgcacgc ggcggcgtgc cagggcagca ggtggatcgg cgggggcgcg
     4921 agcgcgtctt ccaccgcggc ttcggcgtcg gtcgtcgtca cgtgccggtc tccgcggcct
     4981 cgagcaggcg cgcgagcgcc tcgcgcacgc aggcctcgac gtcgtcgggc gcgcggctcg
     5041 cgtcgacgac gcgaaagcga gccggctcgc gcgccgcccg ctgcaggtag cagcggcgca
     5101 cgcgctcgaa gaaatcgctg cgctcgctct cgaaacgatc cggccccacg ctgccgttgc
     5161 gtgcgtgcgc gcgtcgcatg ccgacctcga cgggcgcgtc gagcagcagg gtcaggtcgg
     5221 gctgccggcg cgggtgcacg atgcggacca gcgcgtcaat gtcggcctcg gcaacgcccc
     5281 gcccgccgcc ctgataggcg taggtcgcat ccgtgaagcg atcgcagagc acccaggccc
     5341 cgcgcgccac ggccggctcg accaggtttg cgaggtgact cgcgcgtgcg gcaaacatca
     5401 gcagcagctc gcaggcctgc ggcattggct cgtcgctgcg ct
//
LOCUS       DASPFR010000308         3535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_332976, whole genome shotgun sequence.
ACCESSION   DASPFR010000308 DASPFR010000000
VERSION     DASPFR010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332976"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="VD737_08050"
     CDS             <1..1044
                     /locus_tag="VD737_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10550.1"
                     /translation="YDCVTCGASRSTCASLTQRRRPAFRSPASLVANVRALAQVSRAP
                     IYLVGDVLQAGQAAGHELLERLGRERITNELVFEFLDLPPESYLRAIGRHLRRWSMEI
                     SPESHDPSLRRALDGETSYDNEHLEAAFAEALRHGCRRIDLFFMIGLPGQTHASVMDT
                     VEYCGRLFGRFDRRLACFISPMGPFLDPGSRGFEDPERHGYRLFARTLEEHRQLLVQP
                     TWERILNYETRWMTRRELVDATYDAAEQLNALKARHRRVSAREARSVARRIAAARALR
                     VRLDQAIAGGTEVPRELQGEINRYSVSTVCDKRELFWPRHLFNFRPIGVARLAAGLWR
                     TAGQPGPREAATG"
     gene            complement(1051..>3535)
                     /locus_tag="VD737_08055"
     CDS             complement(1051..>3535)
                     /locus_tag="VD737_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXW10551.1"
                     /translation="VDPFPSIGDVFFLAFYPLVFAAVLTVMRAAALRVPWARLGLDAT
                     ILLLGFGAFFWYCVIEPTATAQRDPDVLKYVLAQSYVGLNCITLLACGVLLMHAGAAP
                     VRRRTVMLLTLGFSCMSFADIIWAMSKVQGSYLPGAASDALYLSCYVWLGAAAREHLR
                     DEPDARRPPNALSNAVVRGMPYIAMIISFSVLVYVESSSATSPVNTMTVIIFVLTLLV
                     MVRQGVISRDDALLRERRAAGLVEARYASLIKNATDVIVICSADGQLRFASPAARRTL
                     GVDPEVVVGRSLLDLWTDGDRDRLAAFLAEVAASPQGRVVGPIELVVETRDRRGTLEC
                     VGSNLADDSAIAGLTLNLRDVSERKVLEEQLRKLAFHDPLTLLANRSLFWNRVEHALA
                     LAQRTQQHVAVMFLDLDNFKNVNDSLGHDAGDRLLQAAAQRLVKSTRPSDTVARLGGD
                     EFAILLEGLRDERDVERLAGLVSASFGRPLLVDGRETETTASIGVACSRPGDDAEQLL
                     RNADIAMYSAKAAGKARCVVFQPHMQEQLHDRLRLEQDIDLALTRNEFFVEFQPVIDL
                     TTRELLGVEALVRWQHPERGRVMPGSFIPVAEESGRIVELGRQVLVDACRQVRRWSEE
                     LPHGRGLRVAINISGRHLQQGDLIGDVRHALEVSGLDPGNLVIELTENTIMQNTEANL
                     DRFRELKALGVRLAIDDFGVGYSSLSYLHRFPIDILKIDRAFVSRLTEQDGGPELARA
                     VVMLGSTLGLETVAEGIENEEQVRSLLDLGCVAGQGFLFASSSSLEDVGRTPYMARRA
                     RIRGEHGPVDLTATGRFRTTDLWPRSAAG"
BASE COUNT          546 a         1209 c         1271 g          509 t
ORIGIN      
        1 tacgactgcg tcacgtgcgg cgcctcgcgc tcgacctgcg cctcgctgac gcagcgccgg
       61 cgccccgcgt ttcgttctcc cgccagcctg gtggccaacg tgcgcgcgct cgcgcaggtg
      121 tcgcgcgcgc cgatctacct ggtgggtgac gtgctgcagg cgggacaggc ggccggtcac
      181 gaactgctgg agcggctcgg ccgcgagcgc atcacgaacg aactcgtctt cgaattcctc
      241 gacctgccgc cggaaagcta cctgcgcgcg atcggccgcc acctccgtcg ctggagcatg
      301 gagatcagcc cggaaagcca cgatccgtcg ctgcgacggg cgctcgacgg cgagacttcc
      361 tacgacaacg agcacctgga agcggcgttc gccgaggcgt tgcgacacgg ctgccgacgc
      421 atcgacctgt tcttcatgat cggcctgccg ggccagacgc acgcgtcggt catggacacg
      481 gtggagtatt gcgggcggct gttcgggcgc ttcgaccgcc gcctggcctg tttcatctcg
      541 ccgatggggc cgttcctcga tccgggcagc cggggcttcg aagatccgga gcgtcatgga
      601 taccggctct tcgcgcgcac gctggaagag catcggcagt tgctggtgca gccgacgtgg
      661 gagcggatcc tcaactacga gacacgctgg atgacgcggc gcgagctggt cgatgcgacc
      721 tacgacgccg ccgagcagct gaacgcgttg aaagcgcggc atcggcgcgt gtcggcgcgc
      781 gaggcccggt cggtcgcccg gcggatcgcg gccgcgcgcg cgctccgggt ccggctcgac
      841 caggcgatcg ccggtggcac ggaggtgccc cgggaactcc agggcgagat caaccgctac
      901 agcgtgtcga cggtctgcga caagcgcgaa ctgttctggc cgcgccacct gttcaacttc
      961 cggccgatcg gtgtcgcgcg gctcgccgcc gggctctggc ggacggcggg gcagccaggt
     1021 cctcgcgagg ccgcgacggg ctgatcgagg tcagccggcc gccgaacgcg gccagaggtc
     1081 ggtggtcctg aagcggccgg tggcggtcag gtcgaccggg ccgtgctcac cgcggatgcg
     1141 cgcgcgtcgt gccatgtagg gcgtccggcc gacgtcttcg agcgacgacg agctcgcaaa
     1201 caggaagccc tgtcccgcga cgcagccgag gtccagcagg ctgcgcacct gctcctcgtt
     1261 ttcgatgcct tcggccacgg tctcgagccc gagcgtggag ccgagcatca ccacggcgcg
     1321 ggcgagttcc ggtccgccgt cctgctccgt cagccggctc acgaacgcgc ggtcgatctt
     1381 gaggatgtcg atcgggaagc ggtgcaggta cgaaagcgag gagtagccca cgccgaagtc
     1441 gtcgatggcg aggcgcacgc ccagcgcctt gagctcacgg aagcggtcga ggttggcctc
     1501 cgtgttctgc atgatcgtgt tctcggtcag ctcgatcacg aggttgccgg ggtcgaggcc
     1561 ggacacctcg agcgcgtgcc gcacgtcgcc gatcaggtcg ccctgctgca ggtgacggcc
     1621 cgagatgttg atcgccacgc gcagcccgcg tccgtgcggc agctcctcgc tccatcggcg
     1681 cacctgccgg caggcgtcca ccagcacctg ccggcccagc tcgacgatcc ggccggactc
     1741 ctcggccacc gggatgaagc tgcccggcat gacgcgcccg cgctcggggt gctgccagcg
     1801 caccagcgcc tcgacgccga gcagttcgcg cgtggtcagg tcgatgacgg gctggaactc
     1861 cacgaagaac tcgttgcgcg tcagcgccag gtcgatgtcc tgttccaggc gcagccggtc
     1921 gtgcagctgc tcctgcatgt ggggttggaa caccacacag cgagccttgc ccgccgcctt
     1981 ggcgctgtac atcgcgatgt cggcgttgcg cagcagctgc tcggcgtcgt caccgggccg
     2041 cgagcaggcg acgccgatgc tcgccgtcgt ctcggtctcg cgcccgtcca cgagcagcgg
     2101 ccggccgaac gacgcggaga ccaggccggc gaggcgctcg acgtcccgct cgtcgcgcag
     2161 cccctcgagc aggatcgcga actcgtcgcc gcccaggcga gccacggtgt cggacggccg
     2221 cgtcgacttg accagccgct gggcggccgc ctgcagcagc cgatcgccgg cgtcgtggcc
     2281 gagcgagtcg ttgacgttct tgaagttgtc gaggtccagg aacatgacgg cgacgtgctg
     2341 ctgcgtgcgc tgcgccagcg cgagcgcgtg ctcgacacgg ttccagaaca ggctgcggtt
     2401 cgcgagcagc gtgagcgggt cgtggaaggc gagcttgcgc aactgctcct cgagcacctt
     2461 gcgctcgctg acgtcgcgca ggttcaacgt gagaccggcg atcgcggaat cgtccgcgag
     2521 gttgctgccg acacactcga gcgtgccgcg gcggtcgcgc gtctcgacga cgagttcgat
     2581 cgggcccacc acgcggccct gcggactggc ggcgacttcg gcgaggaacg cggccaggcg
     2641 atcacggtcg ccgtcggtcc agaggtcgag caggctgcgg cccacgacga cctcggggtc
     2701 gacgccgagc gtgcgtcggg ccgccggcga ggcgaagcgg agctggccgt ccgcgctgca
     2761 gatcacgatc acgtcggtcg cgttcttgat cagcgacgcg tagcgggcct cgaccagacc
     2821 ggcggcacgg cgctcccgca gcagcgcatc gtcccgcgag atcacgccct ggcgcaccat
     2881 gaccagcagc gtcagcacga agatgatcac cgtcatggtg ttgacggggc tcgtggcaga
     2941 actgctttcg acgtacacca gcaccgagaa cgagatgatc atcgcgatgt agggcatgcc
     3001 gcgcacgacg gcgttgctga gcgcgttcgg cggccggcgc gcgtcgggtt cgtcgcgcag
     3061 gtgttcgcgc gcggccgcgc cgagccagac gtagcacgag aggtagaggg cgtcggaggc
     3121 cgcgcccggc aggtagctgc cctgcacctt ggacatcgcc cagatgatgt ccgcgaagga
     3181 catgcacgag aacccgaggg tcagcagcat gacggtgcgg cgccggacgg gcgcggcgcc
     3241 cgcgtgcatc agcaacacgc cgcaggcgag cagcgtgatg cagttgaggc cgacgtagct
     3301 ctgcgcgagc acgtacttga gcacgtccgg gtcgcgctgc gcggtcgcgg tcggctcgat
     3361 cacgcagtac cagaagaacg cgccgaaacc gagcagcagg atcgtcgcat ccagcccgag
     3421 ccgcgcccac ggcacgcgca acgcggcggc gcgcatgacg gtgagcacgg ccgcgaacac
     3481 gagcgggtag aaggcgagga agaagacgtc gccgatcgac ggaaacgggt cgacg
//
LOCUS       DASPFR010000309         6996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_697801, whole genome shotgun sequence.
ACCESSION   DASPFR010000309 DASPFR010000000
VERSION     DASPFR010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6996
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697801"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1917)
                     /gene="dxs"
                     /locus_tag="VD737_08060"
     CDS             complement(13..1917)
                     /gene="dxs"
                     /locus_tag="VD737_08060"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HXW10552.1"
                     /translation="MSSSDLYPLLARIESPADLRKLPATRLGPVADELREFLIQTVSQ
                     MGGHFAAGLGTVELTVALHYVFDTPHDRLVWDVGHQAYPHKVLTGRRDRLRTIKLKGG
                     LAPFPSRFESEYDTFGVGHSSTSISAAIGMALAAQAKGEKRRVVAVIGDGAITAGQAF
                     EALNHAGALDANILVVLNDNDMSISENVGALSNYFARILSGKTYAALREGGKKILRRM
                     PTVWELARRSEEHFKGMVLPGTMFEEMGFNYFGPIDGHDLDSLVKTLNNLKDVEGPQF
                     LHVVTRKGKGYAPAEADPIKWHGPGPFDPTSGTIHKEKTTGPVYSQVFGQWLCDAAAS
                     DPRVVAITPAMREGSGLVAYSKRFPERYFDVAIAEQHAVTLAAGFACEGLRPVVAIYS
                     TFLQRAYDQLIHDVALQELPVLFAIDRGGLVGGDGATHQGAFDLSYLRCIPNMVVMAP
                     ADENECRQMLYTGLTLDGPSAVRYPRGQGPGVPIVERMEALPVGRAEVRREGRSGLLL
                     LAFGTMVEPCVAVADKLDATLVNMRFVKPLDEDTVCRLAASHACVITLEENVVAGGAG
                     SAVAECLAQRGLESVVHHIGIPDRFIEHGTREDGLVMAGLDAAGIERQVLRTWAAQAP
                     ARPVVAGRAS"
     gene            complement(1958..2890)
                     /locus_tag="VD737_08065"
     CDS             complement(1958..2890)
                     /locus_tag="VD737_08065"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HXW10553.1"
                     /translation="MAVAPSPGAPADVAPELDRALVRWRERVERQLDRWLPPATALPE
                     RLHAAQRYSVLGSGKRIRPALVYATAETLGVPIEQVDAAACAVELIHCYSLVHDDLPA
                     MDDDDLRRGRPTCHRAFDEATAILAGDSLQVLAFQLLATHPGAPAEPAVRVAIIELLA
                     VASGTAGMAGGQALDLAAEGRALQLDEIERVHALKTGALIRASVLMAARCSPTLSATE
                     FDALSRLGAAVGLAFQVQDDILDVEGDVAVLGKTPGSDQARGMPTYPAIAGMEAARGR
                     VRALHEEATRLLQRHGWHDGPLAALTDWLLTRNR"
     gene            complement(2890..3132)
                     /gene="xseB"
                     /locus_tag="VD737_08070"
     CDS             complement(2890..3132)
                     /gene="xseB"
                     /locus_tag="VD737_08070"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002137.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HXW10554.1"
                     /translation="MTESSTRPPDFERALAELEATVDKLEHGELSLEDALKDFERGVA
                     LARECQAALKQAEQKVEVLLQKTPTAQPEAFEPDDE"
     gene            3396..3809
                     /locus_tag="VD737_08075"
     CDS             3396..3809
                     /locus_tag="VD737_08075"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HXW10555.1"
                     /translation="MSLKGTRTEENLKAAFAGESQANRRYLYFAQKADVEGFNDVAAV
                     FRSTAEGETGHAHGHLEYLEAVGDPATGLPIGPTQSNLKASIAGETHEYTDMYPGMAK
                     TARDEGFDEIADWFETLAKAERSHANRFQKALDAL"
     gene            3922..5295
                     /locus_tag="VD737_08080"
     CDS             3922..5295
                     /locus_tag="VD737_08080"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HXW10556.1"
                     /translation="MTDDITPMDSSPASAREGSLAQPTRHAIAWREPEFYDEPALMHE
                     LERVYELCHGCRRCFSLCNAFPLLFDAIDESPTGELDSVDRKVFWEVTDHCYLCDMCY
                     MSKCPYVPPHPWNIDFPHLMLRAKAVRFRKGEFRFRDKLLSSTDLVGSLAGIPVVSQV
                     VNKVNDTAAGRRLLERTLGVHVEAPIPDYHSTSGRRRVERGDRRGAVEARATAETRGQ
                     VALFATCYCNRNEPDVADDLATVFRHNDIRVVLAPKEKCCGMPKLELGDLDSIERAKD
                     VNVPELARMVDQGYDLVAPVPSCVLMFKQELPLLFPEDEQVRKVQAAMFDPFEYLALR
                     HKAGLLRTDFKQPLGRVSYHVPCHLRVQNLGLKTRDVLKLVPGTEIEPIERCSGHNGT
                     YGVKKEFRETSMKIGRPVIGKVRNAAPDHYASDCPMAGHQIESGLGADAKPPTHPLKL
                     LRLAYGI"
     gene            5331..5948
                     /locus_tag="VD737_08085"
     CDS             5331..5948
                     /locus_tag="VD737_08085"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HXW10557.1"
                     /translation="MTNLNGSTPAGHQKLRAEDLYGLEQYAKQRAQFRPQVLAHKKRR
                     SLQVGPNATFCFEDRLTVQYQVQEMLRIERIFEAAGIEDELGAYNPLIPDGSNWKATL
                     LIEYPDPDVRKAALARLKGIEDRCWVQVAGHERVYAIADEDLDRENEEKTSSVHFLRF
                     ELTPPMVAEAKRGAAISVGVDHDHYRHAVEPVEDAVREALVADFA"
     gene            6064..6393
                     /locus_tag="VD737_08090"
     CDS             6064..6393
                     /locus_tag="VD737_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10558.1"
                     /translation="MARFAGSTGRVHDTRPGWVTRLRDRIHPRYVDGSDLPDEPASPY
                     RARLHVPEEMTPYGMEVTEREVVRGTVRVVYEIRCSCRRRWFQRALERVQVCPRCARA
                     VLVERPD"
     gene            6562..>6996
                     /locus_tag="VD737_08095"
     CDS             6562..>6996
                     /locus_tag="VD737_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXW10559.1"
                     /translation="MSQSYTAQDIEVLSGLEPVRRRPGMYTDTSRPNHLAHEVVDNSV
                     DEALAGHCSRIDVTVYKDGSLEVADDGRGMPVDIHPKEKVSGVELILTRLHAGGKFSA
                     DSGYKFSGGLHGVGVSVVNALSKHLECWVRRGGKEYNISFKNG"
BASE COUNT         1141 a         2381 c         2415 g         1059 t
ORIGIN      
        1 gcccccggcg cgtcaggacg cgcggcccgc gaccaccggg cgcgccggtg cctgcgccgc
       61 ccaggttcgc agaacctgtc gctcgatgcc cgcggcatcc aggccggcca tcaccagccc
      121 gtcctcgcgc gtgccgtgct cgatgaaccg gtccgggatg ccgatgtggt gcacgacgct
      181 ctcgaggcca cgttgcgcca ggcactcggc caccgcgctg ccggcgccgc cggccaccac
      241 gttttcctcg agcgtgatca cgcacgcgtg gctggccgcg aggcgacaca cggtgtcctc
      301 gtcgagcggt ttcacgaagc gcatgttcac gagcgtcgca tcgagcttgt cggccacggc
      361 gacgcaaggc tcgaccatgg tcccgaacgc gagcagcagc agtccggacc ggccttcgcg
      421 ccgcacctcg gcgcggccga ccggcaaggc ctccatccgc tcgacgatcg gcactccggg
      481 gccctggccc cgcgggtagc gcacggcgga cgggccgtcg agcgtgagtc cggtgtagag
      541 catctggcga cactcgttct cgtcggccgg cgccatgacg accatgttcg ggatgcagcg
      601 caggtacgag agatcgaacg cgccctggtg cgtggcgccg tcgccgccga ccagcccgcc
      661 gcggtcgatc gcgaacagca cgggcagctc ctgcaacgcg acgtcgtgga tcagctggtc
      721 gtaggcccgc tgcaggaaag tcgagtagat cgcgacgacg ggccgcaggc cctcgcaggc
      781 gaacccggcc gcgagcgtca ccgcgtgctg ctcggcgatc gcgacgtcga agtagcgctc
      841 cggaaagcgc ttcgaatatg cgacgagccc ggagccttcg cgcatcgcgg gcgtgatcgc
      901 cacgacgcgc ggatcgctgg cggcggcgtc gcagagccac tggccgaaca cctgcgaata
      961 cacggggccg gtcgtctttt ccttgtggat cgtgccgctg gtcgggtcga acggacccgg
     1021 tccgtgccac ttgatcgggt ccgcctcggc cggcgcgtag cccttgccct tgcgcgtgac
     1081 cacgtgcagg aactgcgggc cctcgacgtc cttcaggttg ttgagcgtct tgacgagcga
     1141 gtcgaggtcg tggccgtcga tcgggccgaa gtagttgaac cccatctcct cgaacatcgt
     1201 gccgggcagc accatgccct tgaagtgctc ctcggagcgg cgggcgagtt cccacaccgt
     1261 cggcatgcgg cgcaggatct tcttgccacc ctcgcgcagc gccgcatagg tcttgccgga
     1321 gaggatgcgc gcgaagtagt tcgagagcgc gcccacgttc tccgagatcg acatgtcgtt
     1381 gtcgttgagc acgaccagga tgttcgcgtc gagcgcaccg gcgtgattga gcgcctcgaa
     1441 cgcctggccc gccgtgatcg ccccgtcgcc gatcacggcg acgacgcgcc gtttttctcc
     1501 cttggcctgg gccgcgagcg ccatgccgat ggcggcgctg atcgaggtgc tcgagtgccc
     1561 gacgccgaag gtgtcgtact cgctttcgaa gcgactcggg aacggcgcga gcccgccctt
     1621 gagcttgatc gtgcgcaggc ggtcgcggcg cccggtcagc accttgtggg gatacgcctg
     1681 gtggcccacg tcccagacca gcctgtcgtg gggcgtgtcg aagacgtaat gcagcgcgac
     1741 ggtcagttcg accgtaccga ggccggccgc gaaatgccca cccatctggc tgacggtctg
     1801 gatcaggaac tcccgcaatt cgtccgcgac cggaccgagc cgggtggccg gcagcttgcg
     1861 caggtcggcg ggcgactcga tccgggcgag cagggggtac aggtcggagg acgacatttc
     1921 gctgaatttt acaccgagcc cgggcagggc cgtccgacta gcggttccgg gtcagcagcc
     1981 agtcggtcag ggccgccagg ggcccgtcgt gccagccgtg ccgttgcagc agcctggtgg
     2041 cctcctcgtg cagggcgcgg acgcggcccc gggcggcctc catcccggcg atggccgggt
     2101 aggtcggcat gccgcgggcc tggtcgctgc ccggcgtctt gccgagcacg gccacgtcgc
     2161 cttcgacgtc caggatgtcg tcctgcacct ggaaagcgag cccgaccgcg gcgcccagcc
     2221 gtgacagggc gtcgaactcc gtggcgctca gcgtcgggct gcagcgtgcc gccatcagca
     2281 cgctggcgcg gatcagcgcg ccggtcttca gcgcgtggac gcgttcgatc tcgtcgagtt
     2341 gcagggcccg gccttcggcg gcgaggtcaa gcgcctgtcc accagccatg ccggccgtgc
     2401 cactcgcgac cgccagcagt tcgatgatgg cgacgcgcac cgcgggctcc gccggcgccc
     2461 cggggtgcgt cgcaagcaac tggaacgcga gcacctgcag cgagtcgccg gccaggatgg
     2521 ccgtcgcttc gtcgaaggcc cggtggcagg tgggccggcc gcggcgcagg tcgtcgtcgt
     2581 ccatcgcggg caggtcgtcg tgcacgagcg aatagcagtg gatcagctcc acggcgcagg
     2641 cggccgcgtc gacctgctcg atcggcacgc cgagcgtttc cgccgtcgcg tagaccagcg
     2701 ccggccggat acgcttgccg gagccgagca cgctgtagcg ttgcgccgcg tgcagtcgct
     2761 cgggcaacgc cgtcgcgggc ggcagccagc ggtcgagttg ccgctcgacg cgctcacgcc
     2821 agcgcacgag cgcacggtcg agctccggcg cgacgtccgc gggcgcaccg ggactcggtg
     2881 ctacggccat cactcgtcgt ccggctcgaa cgcttccggt tgcgcggtcg gcgtcttctg
     2941 cagcaggacc tccaccttct gctcggcctg cttgagtgcc gcctggcact cgcgggcgag
     3001 cgccacgccg cgctcgaagt ccttgagcgc gtcctcgagc gacagctcgc cgtgctcgag
     3061 cttgtcgacg gtggcctcga gctccgcgag ggcgcgctcg aagtcgggtg ggcgggtcga
     3121 agattcggtc acggcaggcc tccggagggc gcgcagcctt gcacaggggt gtggggcggt
     3181 caacgcggca tcggtgcacc ggtgcacgtg caagccgcag cggcaaacgg cgcggatcgt
     3241 gggtatcgta tgcagagacg ccccggcgcc gcattcgcct cctgattgcc caggtcgatt
     3301 gactcgaaat tagacttggt ctaaatactt gtccgaccgt ttcagggcgc gtctgcgggc
     3361 gctacaaaca tcgagagtga aggagtggag gcatcatgtc gctcaaggga accaggaccg
     3421 aagagaatct gaaggctgct tttgccggcg aatcccaggc caatcgccgt tacctctact
     3481 tcgcacagaa ggcggacgtc gagggcttca acgacgtcgc ggcggtgttc cgctcgaccg
     3541 cggagggcga gaccggtcac gcacacggtc acctcgaata cctggaggcc gtgggcgacc
     3601 ccgccaccgg gctgccgatc ggcccgaccc agtccaacct gaaggcctcg atcgcgggcg
     3661 agacgcacga gtacaccgac atgtacccgg gcatggcgaa gacggctcgc gacgaaggct
     3721 tcgacgagat cgccgactgg ttcgagacgc tcgcgaaggc ggagcggtcg cacgccaacc
     3781 ggttccagaa ggcgctcgac gcactctgac cccgacggac gttcgatcca ggaccaggca
     3841 ccgtgccggg agggcggacc tagcatccgc ccttccgggg agcgcggcgc cgggacacgc
     3901 gttgtgcctt tggggttgcg catgaccgac gacatcacgc cgatggattc gagccccgcc
     3961 tcggcgcgcg agggcagcct ggcgcagccg acgcgccatg cgatcgcgtg gcgcgagccg
     4021 gagttctacg acgagccggc gctgatgcac gaactcgagc gcgtctacga gctgtgccac
     4081 gggtgccgtc gctgcttcag cctctgcaac gcgttcccgc tgctgttcga cgcgatcgac
     4141 gagtcgccga ccggcgaact cgactcggtc gaccgcaagg tcttctggga ggtcaccgac
     4201 cactgctacc tctgcgacat gtgctacatg tccaagtgcc cgtacgtgcc gccacatccg
     4261 tggaacatcg actttccgca cctgatgctg cgcgccaaag cggtcaggtt ccgcaagggc
     4321 gagttccgct tccgcgacaa gctgctgagt tccacggacc tggtcggctc gctcgcgggc
     4381 attcccgtcg tgtcgcaggt cgtgaacaag gtgaacgaca cggccgccgg ccggcgcctg
     4441 ctcgagcgga cgctcggcgt gcacgtcgag gcgccgatcc ccgattacca cagcaccagc
     4501 ggtcgccggc gcgtcgagcg cggtgatcgc cgcggcgccg tcgaagccag ggccacggcc
     4561 gagacgcgcg ggcaggttgc gctgttcgcg acctgttact gcaaccgcaa cgaacccgac
     4621 gtcgccgacg acctggcgac ggtgttcagg cacaacgaca tccgggtggt gctggcgccg
     4681 aaggaaaagt gctgcggcat gccgaaactc gagctcggcg acctcgattc gatcgagcgc
     4741 gcgaaggacg tcaacgtccc ggagctagcg cgcatggtgg accagggcta cgacctcgtc
     4801 gcgcccgtgc cgtcgtgcgt gctgatgttc aagcaggaac tgccgctgct gtttcccgaa
     4861 gacgagcagg tcaggaaggt gcaggcggcg atgttcgatc cgttcgaata cctcgcgctg
     4921 cggcacaagg ccgggctgct gcgcacggac ttcaagcagc cgctcggcag ggtcagctat
     4981 cacgtgccct gccacctccg cgtgcagaac ctggggctca agacgcgcga cgtgctcaag
     5041 ctcgtgccgg gcaccgagat cgagcctatc gagcgttgct cgggtcacaa cggcacgtac
     5101 ggcgtcaaga aggagtttcg cgagacgtcg atgaagatcg gccggccggt gatcggcaag
     5161 gtcaggaacg cggcgcctga ccactacgcg agcgactgcc cgatggcggg ccaccagatc
     5221 gaatccgggc tcggcgccga cgcgaagccg ccgacgcatc ccctgaagct gctgcgcctc
     5281 gcctatggca tctgaatgaa ccgcgagaga cacgaagacc ggacgtcccc atgaccaacc
     5341 tgaacggctc gacccccgcc gggcaccaga aactgcgggc ggaagacctc tacggcctcg
     5401 agcagtacgc gaagcagcgc gcgcagttcc gtccgcaggt gctcgcgcac aagaaacgcc
     5461 ggtcgctgca ggtggggccg aacgcgacgt tctgcttcga ggatcggttg acggtccagt
     5521 accaggtgca ggagatgctg cgcatcgagc gcatcttcga ggccgcgggg atcgaggacg
     5581 aactcggcgc gtacaacccg ctgatcccgg acggcagcaa ctggaaggcg acgctgctga
     5641 tcgagtatcc ggatcccgac gtgcgcaagg ccgcgctcgc ccggctcaag ggcatcgagg
     5701 atcgctgctg ggtgcaggtc gccggccacg agcgggtcta tgcgatcgcg gacgaggacc
     5761 tcgaccgaga gaacgaggag aagacctcgt ccgtgcactt cctgcgcttc gaactcaccc
     5821 cgccgatggt ggccgaagcg aagcgaggcg cagcgatcag cgtcggcgtg gaccacgacc
     5881 actatcgaca cgcggtcgag ccggtggagg atgcggtccg cgaggcgctc gtcgcggatt
     5941 tcgcctgaaa attaccggtg ccataagtca aatcgacgcc tgcgcggcgc ggcccgcgct
     6001 aagctcgatc gcacaaccag aacaggggac gggcggcgcg ggaaggcggt gcccggcacg
     6061 tgcatggctc gattcgcagg ctctactggg cgagttcacg acacccggcc gggatgggtc
     6121 acgcggttgc gcgaccgcat ccacccccgt tacgtcgacg gctccgacct gccggacgag
     6181 ccggcctcgc cgtaccgcgc gcgcctgcac gtgcccgagg agatgacgcc gtacgggatg
     6241 gaagtgaccg agcgcgaagt cgttcgcggc acggtccgcg tcgtttacga gatccgctgc
     6301 agctgccgtc gccggtggtt ccagcgcgcg ctcgaacgcg tgcaggtgtg cccgaggtgc
     6361 gcgcgggccg tgctcgtgga acgtcccgac tagcgaaacg ccctccggaa ttggggacac
     6421 tcctgaatta ccgcggtaat tcaggcgtgt ccccattttc gttggaattc aggagtgtcc
     6481 ccatttctcc gcgggcgggg ggcgtctgct accatgcgcc cgcctttttc gacccacgcg
     6541 agcccgcgga ccgatcccag aatgagccag tcctataccg ctcaagacat cgaagtcctg
     6601 agcggcctcg agcccgtccg gcgtcgcccg ggcatgtaca ccgacaccag tcggcccaac
     6661 cacctcgccc acgaagtcgt cgacaacagc gtcgacgagg cgcttgccgg ccactgcagc
     6721 cgcatcgacg tgaccgtcta caaggacggc tcgctcgagg tggcggacga cgggcgcggc
     6781 atgccggtcg acatccaccc gaaggagaag gtgagcggcg tcgaactgat cctgacgcga
     6841 ctgcacgcgg gcgggaagtt ctcggccgac tcgggctaca agttctcggg cggcctgcac
     6901 ggcgtcggcg tgtcggtggt caacgcgctc tcgaagcacc tcgagtgctg ggtgcggcgc
     6961 ggcggcaagg aatacaacat ctcgttcaag aacggg
//
LOCUS       DASPFR010000310         5400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1552913, whole genome shotgun sequence.
ACCESSION   DASPFR010000310 DASPFR010000000
VERSION     DASPFR010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552913"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VD737_08100"
     CDS             complement(<1..715)
                     /locus_tag="VD737_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXW10560.1"
                     /translation="MSLPLTALDREIAQLRERLEGAEEMRRVVSAGPRDGGGPTAAGP
                     VARLAGARPARASTPAESSSPAAVTVSEAGNVLHANQRFAAFTGRTLRELYSTPIADL
                     VVAADRSRVGELLDSGRRDATLDTVVLRPDGTRRPARFATIDVAHGSTSFLLVDLEEE
                     SRRNEAESAVAAITTGDIDAVVVGGDQVILVADADRSYRDLVERIGQGAVSLTHRGEV
                     LYANEPFGAMLGLRREDVLG"
     gene            complement(729..1067)
                     /locus_tag="VD737_08105"
     CDS             complement(729..1067)
                     /locus_tag="VD737_08105"
                     /inference="COORDINATES: protein motif:HMM:NF019309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HXW10561.1"
                     /translation="MTGPLTLSEIAAAAGGHHLSDQQWELRLYTAGQTPKSIAAFNNL
                     KRICEEYMPGRYEIEVVDLMQNPRLAKEDQIVAVPTLVRKLPQPLRKIIGDLSDTERA
                     LVGLQLRPRA"
     gene            complement(1082..2833)
                     /gene="kaiC"
                     /locus_tag="VD737_08110"
     CDS             complement(1082..2833)
                     /gene="kaiC"
                     /locus_tag="VD737_08110"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HXW10562.1"
                     /translation="MSGETVDRLTTIKKAGVTAPPPDLPTLPKAPTGIDGLDEILSGG
                     FPAGRPTLVCGSAGCGKTLLSMEFLVRGALEFDEPGAYISFEETEAELAQNVASLGFD
                     VDALVEQGKLAFDHVKVERQEIEETGAYDLDGLFVRLGLAIDSVGARRVVLDTIESLF
                     SGFSDAAVLRAELRRLFGWLKERGVTAVITGERGDGVLTRQGLEEYVSDCVILLDHRV
                     LDQVSTRRLRVVKYRGTAHGTNEYPFLIDESGITVLPVTSLGLKHRASTERISTGIPR
                     LDAMLGGQGYYRGSSILVSGTAGAGKTSLAAHFAAAVCARGERCLYLAFEESPDQIVR
                     NMRSIGVDLGEPAAKGLLRLEASRPTYQGLEMHLSQIHRLVAQFDPHAVVIDPVSNLS
                     SAGAVLDATATLLRAIDFLKSRGTTALLTNLTNVNAALESTDIGISSLIDTWIVLRDI
                     EAGGERNRGLYVIKSRGMAHSNQIREFLITSEGIDLQDVYVGADGVLTGSLRAAQEAR
                     ERAAAVAREQEAQRRSRELERRRTALEAQIRVLQAEIGSLEGEGTIADQQFEERSATI
                     EQDRAAAALRRGADAGR"
     gene            complement(2946..5234)
                     /locus_tag="VD737_08115"
     CDS             complement(2946..5234)
                     /locus_tag="VD737_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXW10563.1"
                     /translation="MLAWILLGVLAAVLAGLLVRRRREAHRFGDLAERLQAVARSGDL
                     AERLAPHVGDGRPAEIAEGVDRLIERLQVESTSRDERESIYRRMLESMHEAMLVVRDG
                     IHLANPRFAELCGVSGPDKLYGRRLEDLVHPDYAELVGEYLRRHISGQSVLQRLEVEL
                     LAEAPGPAPRLELTFSRASLDGRPAVLVTGVEMSPRHAHEAPRSHANAWEALDSLAES
                     VLTTDAEGRIVYANKAAEQLINKPAGEVIGRSFADVVHLVDEGDRKAPGDPVRQSITT
                     GQRVNLGRRGMLLTNNGNGERSIEITVSPMRDVAGRVDGTVIALRDVSELRGLTRAMS
                     YQASHDGLTGLVNRREFERRLEEALESTRSGARHVLCYLDLDRFKAVNDESGTHVAGD
                     ALLREIAALIKDAVRDSDTVGRLGGDEFGVLLAGCPLDKARQIADDVVRAVADHRFVW
                     KDRIFSIGVSVGLVEVTVESSSIDDLMQAADSACYVAKNQGGRVHVYSARDEAAARQR
                     GEIVWLQLIQSALKEDSNRFELHAQPIVPVSSSPDAGGPGLEVLLRLKDDKGASISPS
                     EFMRAAERYRLMPHVDRWVVQTAFTLLGRGAVRLAPERSLCINLSGQTLGDAAFLEFV
                     VDCLDRTGVAPSRICFEVTENSVITNIEHARRFIGVLHGMGCRFALDDFGRGLSSFAN
                     LKNLSLDYLKIDGSFIRNLASDNVNQAMVTAMIKLARSLNFQVIAEQVEDLSSLDSAR
                     RMGVDFVQGYQVGRPIPLPLAP"
BASE COUNT          796 a         1874 c         1883 g          847 t
ORIGIN      
        1 ggccgagcac gtcctcgcgg cgcaggccga gcatcgcgcc aaagggctcg ttggcgtaca
       61 gcacttcgcc gcggtgggtc aggctcacgg cgccctgccc gatgcgctcg acgaggtccc
      121 ggtacgaccg gtccgcgtcg gcgacgagga tcacctggtc gccgccgacg accaccgcgt
      181 cgatgtcgcc ggtggtgatc gcggcgaccg ccgactcggc ctcgttgcgt cgcgactcct
      241 cttcgaggtc gacgagcagg aacgacgtcg aaccgtgtgc aacgtcgatg gtcgcgaacc
      301 gtgccgggcg gcgggtgccg tcgggccgca gcacgacggt gtcgagcgtc gcatcgcgac
      361 gtccggagtc gagcagctcg cccacgcgcg accgatccgc cgcgaccacg aggtcggcga
      421 tcggcgtcga gtacagttca cgcagcgtgc gaccggtgaa ggcggcgaag cgctggttcg
      481 cgtgcagcac gttgcctgcc tcggacaccg tcaccgccgc cggcgaggac gattcggcgg
      541 gagtgctcgc gcgggcgggc ctggcccccg ccagccgcgc gaccgggccc gccgcggtcg
      601 ggccaccgcc gtcacgaggt ccggcgctga cgacgcgccg catctcctct gctccctcga
      661 ggcgctcgcg cagctgggcg atttcgcgat cgagggccgt gagcggcagc gacatgacgc
      721 tcggcccgtc aggcgcgcgg gcgcagctgc agtccgacca gcgcgcgctc ggtgtcggag
      781 aggtccccaa tgatcttgcg cagcggctgc ggcagcttgc gcaccagcgt cggcacggcg
      841 acgatctggt cttccttcgc gagccgcggg ttctgcatga ggtcgacgac ttcgatctcg
      901 tagcgccccg gcatgtactc ctcgcagatc cgcttcaggt tgttgaacgc ggcgatcgac
      961 ttgggcgtct ggccggcggt gtagagccgg agttcccact gctggtcgct caggtgatgc
     1021 ccgccggcgg ccgcggcgat ttcggacagc gtcaggggtc ccgtcattcg tacttcctct
     1081 gtcatcggcc cgcgtccgcg ccacgccgca gcgcggcggc cgcgcggtcc tgctcgatcg
     1141 ttgccgaccg ttcctcgaat tgctggtccg cgatcgtgcc ttcgccctcg agcgaaccaa
     1201 tctccgcctg cagcacccgg atctgggcct cgagcgccgt gcggcgccgt tcgagttcgc
     1261 gggaacgccg ctgcgcctcc tgctcgcgcg cgacggcggc ggcgcgttcg cgcgcctcct
     1321 gggcggcccg cagcgatccc gtgagcacgc catcggcgcc cacgtagacg tcctgcaggt
     1381 cgataccttc ggaggtgatc aggaactcgc ggatctggtt cgaatgcgcc atgccgcgtg
     1441 acttgatcac gtacaggcca cggttgcgct ccccgcccgc ttcgatgtcg cgcagcacga
     1501 tccacgtgtc gatcagcgat gaaatgccga tgtcggtgga ctcgagcgcg gcgttgacgt
     1561 tggtcaggtt ggtcagcagc gcggtcgtgc cgcgcgactt gaggaagtcg atcgcccgca
     1621 acaacgtcgc cgtggcgtcg aggaccgcgc cggcgctcga caggttgctg acggggtcga
     1681 tgacgaccgc gtgcgggtcg aactgggcca cgagccggtg gatctgcgag aggtgcatct
     1741 cgaggccttg atacgtgggg cgcgaggcct cgaggcgcag caggcccttg gcggccggct
     1801 ctcccaggtc gacgccgatc gaacgcatgt tgcgcacgat ctggtcgggc gactcctcga
     1861 aggcgaggta gaggcagcgc tcgccgcgcg cgcagacggc cgcggcgaag tgcgcggcca
     1921 ggctggtctt gcccgcgccc gccgtgccgg agacgaggat cgatgagccg cgatagtagc
     1981 cctgcccgcc gagcatcgcg tcgagtcgcg ggatgccggt cgagatgcgc tccgtgctcg
     2041 cccggtgctt gagcccgagc gacgtgaccg gcagcaccgt gatgccgctc tcgtcgatca
     2101 ggaacggata ttcgttggtg ccgtgtgccg tgccgcggta cttgaccacc cgcaagcgcc
     2161 gcgtcgacac ctggtccagc accctatggt cgagcaggat cacgcaatcg gagacgtact
     2221 cctcgaggcc ctggcgcgtc agcacgccgt cgccacgctc gccggtgatc accgccgtga
     2281 cgccgcgctc cttgagccag ccgaacaggc gacgcagttc ggcccgcaac accgcggcgt
     2341 ccgagaagcc cgagaacagc gactcgatcg tgtcgagcac cacgcggcgc gcacccacgg
     2401 aatcgatcgc gagcccgagg cggacgaaca ggccgtcgag gtcgtaggcg cccgtctcct
     2461 cgatctcctg gcgctcgacc ttgacgtggt cgaacgcgag cttgccctgc tcgaccagcg
     2521 cgtcgacgtc gaagccgagc gaggccacgt tctgcgcgag ctcggcctcg gtctcctcga
     2581 aggagatgta ggcgccgggc tcgtcgaact cgagcgcgcc gcgcacgaga aactccatcg
     2641 acagcagcgt cttgccgcac ccggcgctgc cgcagaccag cgtcgggcgg ccggccggga
     2701 agcccccgct caggatctcg tcgaggccgt cgatcccggt cggagccttg ggcagggtgg
     2761 gcaggtccgg gggtggggcg gtgacacccg ccttcttgat cgtagtcagt cggtcgaccg
     2821 tctcgccgga catcgcgtcc tcgtcagcgc atgcaaaggt cggcatcgtc gccctgccgc
     2881 ccagcaccgg caagtcggcg cttgcctaca ccgaagcggg ctatcgagga tgtgaacctg
     2941 ccgcgtcagg gcgcgagagg cagcgggatc gggcggccca cctggtagcc ctgcacgaag
     3001 tcgacaccca ttcggcgcgc ggaatcgagc gagctcaggt cttcgacctg ctcggcgatg
     3061 acctggaagt tgagcgaacg cgcgagcttg atcatcgccg tcaccatcgc ctggttgacg
     3121 ttgtccgagg cgaggttgcg gatgaacgag ccgtcgatct tgagatagtc gagcgacagg
     3181 ttcttcaggt tggcgaacga gctgaggccg cgcccgaagt cgtcgagcgc gaaccggcag
     3241 cccatgccgt gcagcacgcc gatgaagcgc cgcgcgtgct cgatgttggt gatcacggag
     3301 ttctcggtga cctcgaagca gatccgcgag ggtgcgaccc cggtgcggtc gaggcagtcg
     3361 accacgaact cgaggaacgc cgcgtcgccg agtgtctggc ccgaaaggtt gatgcagagc
     3421 gagcgctcgg gagcgagccg caccgcgccg cgcccgagca gggtgaatgc cgtctgcacg
     3481 acccagcgat cgacgtgcgg catcagccgg tagcgctcgg ccgcgcgcat gaactcggac
     3541 ggcgagatcg aggcgccctt gtcgtccttg aggcgcagca gcacttcgag gcccgggccg
     3601 cccgcgtccg ggctgctgga caccggcacg atcggctgtg cgtggagctc gaaccggttg
     3661 ctgtcctcct tgagcgccga ctggatcagc tgcagccaga cgatctcgcc gcgctgacgt
     3721 gccgcggcct cgtcgcgcgc ggagtagacg tgcacgcgac cgccctggtt cttcgcgacg
     3781 tagcacgccg aatcggcggc ctgcatcagg tcgtcgatcg agctgctctc gacggtcacc
     3841 tcgaccaggc cgacgctgac gccgatgctg aagatccggt ccttccacac gaaccgatgg
     3901 tcggcgaccg cgcgcaccac gtcgtcggcg atctgccgtg ccttgtcgag cgggcaaccc
     3961 gccagcagca cgccgaactc gtcgccgccg aggcggccga cggtgtccga gtcgcgcacg
     4021 gcgtccttga tcagcgcggc gatctcgcgc agcagcgcgt caccggcgac gtgggtgccg
     4081 gactcgtcgt tgaccgcctt gaagcgatcg aggtcgaggt agcacaacac gtggcgcgca
     4141 ccgctgcgcg tggattcgag cgcttcctcg agccgccgct cgaattcgcg ccgattcacg
     4201 aggccggtca ggccgtcgtg gctcgcctgg tatgacatcg cacgcgtgag tccgcgcagt
     4261 tcgctcacgt cgcgcaatgc gatcacggtg ccgtcgacgc ggcccgcgac gtcgcgcatc
     4321 ggcgagacgg tgatctcgat cgagcgctcg ccgttgccgt tgttcgtgag cagcatgccg
     4381 cggcgaccga ggttgacgcg ctgcccggtc gtgatgctct gccgcacggg atcgccgggg
     4441 gccttgcgat cgccctcgtc gacgagatgc acgacgtcgg cgaacgaccg gccgatcacc
     4501 tcgcctgccg gcttgttgat caactgctcg gccgccttgt tggcgtagac gatgcgccct
     4561 tcggcgtccg tggtcagcac gctctcggca agcgagtcga gggcctccca cgcattcgcg
     4621 tggctgcgcg gagcctcatg cgcgtggcgc ggcgacatct ccacgccggt caccagcacg
     4681 gcgggccgac cgtcgaggct cgcgcgggaa aacgtcagct ccagccgggg ggccgggccg
     4741 ggagcctccg cgagcagctc cacctcgagc cgttgcagca cgctctgccc cgagatgtgg
     4801 cggcgcaggt attcgccgac gagctcggca taatccgggt ggacgagatc ctcgagccgc
     4861 cgtccataca gcttgtccgg cccggagacg ccgcagagtt cggcgaagcg cgggttggcg
     4921 aggtggatgc cgtcgcgcac gaccagcatc gcttcgtgca tcgattcgag catgcgccgg
     4981 tagatgcttt cgcgttcgtc gcgcgacgtg ctctcgacct gcagccgttc gatcaggcgg
     5041 tcgacgccct cggcgatctc cgcgggacga ccgtcgccga cgtgcggcgc gagccgctcc
     5101 gccaggtcgc cgctgcgcgc gacggcctgc agccgctcgg cgaggtcgcc gaaacggtgc
     5161 gcttcgcgac ggcgccgcac caggagacca gccagtaccg cggccagcac tccgagcagg
     5221 atccacgcga gcatcaagct ttgactctcc ggaccgggca tgtcctttcg ggcctcgggc
     5281 ccccgggtcc ccgcaaggtt tgtagttgtt ctcgtccacg ccgctgacgt gccacgtgag
     5341 cataacggaa cggttcgcgc ggacccgcgg gattcaccat aatgcgcatt gattcggctt
//
LOCUS       DASPFR010000311         3029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1553498, whole genome shotgun sequence.
ACCESSION   DASPFR010000311 DASPFR010000000
VERSION     DASPFR010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553498"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VD737_08120"
     CDS             complement(<1..194)
                     /locus_tag="VD737_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004646726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HXW10564.1"
                     /translation="MARQRHDRSSADGGEDAGGDGPAGGGLRIDKWLWCARFFKTRSV
                     AQAAVEGGHVQLGGERIKASR"
     gene            248..1438
                     /locus_tag="VD737_08125"
     CDS             248..1438
                     /locus_tag="VD737_08125"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HXW10565.1"
                     /translation="MDRSFLDRLSAQTDELRATGLFKPERVLKSAQQPVVTLADGREL
                     INLCANNYLALANHPAILEAAREAIERHGYGMASVRFICGTQDVHKELEARLSGFLGM
                     EDTILYSSCFDANGGLFETLLDEQDAVISDSLNHASIIDGIRLCKAQRLRYANDDLDE
                     LETRLKDASKARVKLIATDGVFSMDGVIARLKDICNLADRYGALVMVDDSHATGFVGR
                     GGRGTPEHREVMGRIDILTGTLGKALGGAAGGYTAARREIVAWLRQRSRPYLFSNSIP
                     PVVAATTLRVLDLVESSDDLRSRLRDNTAYFRRRMTESGFKVVAGEHPIAPVMLGDAA
                     LAGRFAEKLLDRGVYVIGFSYPVVPMGKARIRTQMSAGHDRAQLDRAIDAFVAVGREL
                     DVLA"
     gene            1485..2513
                     /gene="tdh"
                     /locus_tag="VD737_08130"
     CDS             1485..2513
                     /gene="tdh"
                     /locus_tag="VD737_08130"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HXW10566.1"
                     /translation="MKALVKSQRAPGIWMENVAEPKVGPNDVMIKVRKTAICGTDIHI
                     YNWDQWAQKTVPVPMHVGHEYVGEIVEIGSEVQGFAIGDRVSGEGHITCGYCRNCRAG
                     RRHLCRNTVGVGVNRPGCFAEYLVIPAFNAFKLPASITDEVASILDPFGNATHTALSF
                     SLVGEDVLITGAGPIGVMAVAIARHVGARHVVITDVNDYRLDLARKMGATRAINVSHQ
                     SLDDTMKDLGMVEGFDVGLEMSGNPSAFRDLLRTMHHGGNVALLGIPPDDTAIDWNQV
                     IFKGLVLKGIYGREMFETWYKMAAMLQSGLDISPIITHRLPVAEFRDGFEIMRSGQSG
                     KVILDWAA"
     gene            2658..>3029
                     /locus_tag="VD737_08135"
     CDS             2658..>3029
                     /locus_tag="VD737_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HXW10567.1"
                     /translation="MAIRRAEAEWTGSLREGKGELALESGAFRGPYTFKSRFEEGKET
                     NPEELIAAAHAGCFTMALTALLARQQIAPTRIHTTAAVHIEQSAGGFAIPKIELRTRA
                     SIPGMTAEKFAALAADAKQNCP"
BASE COUNT          523 a         1057 c          990 g          459 t
ORIGIN      
        1 cgcgaagcct tgatgcgctc cccgccgagc tggacgtggc cgccctcgac cgctgcctgc
       61 gcgacgctgc gcgtcttgaa gaaccgcgcg caccacagcc atttgtcgat gcgcagtccg
      121 ccgcccgcag gcccgtcccc gccggcgtct tcgccgccgt cggcggatga cctgtcgtgc
      181 cgttgtcgtg ccaccggccg attataagtc cctataatcc gcgcttcgca cgcgcaggcg
      241 cccggtgatg gatcgcagct tcctggatcg actgtccgcc cagaccgacg agctccgcgc
      301 cacgggcctg ttcaagcccg agcgcgtgct caagtcggcg cagcagccgg tcgtcacgct
      361 cgccgacggg cgcgagctga tcaacctctg cgccaacaac tacctcgcgc tggccaatca
      421 tcccgcgatc ctcgaggccg cgcgcgaggc gatcgagcgc cacggctacg ggatggcctc
      481 ggtgcgcttc atctgcggga cccaggacgt gcacaaggaa ctcgaggccc ggctgagcgg
      541 gttcctcggc atggaggaca cgatcctcta ctcgtcctgt ttcgacgcga acggcggcct
      601 gttcgagacg ttgctcgacg agcaggacgc ggtgatcagc gactcgctca accacgcgag
      661 catcatcgac ggcatccgcc tgtgcaaggc gcagcggctg cgctacgcca acgacgacct
      721 cgacgagctg gagacgcggc tgaaggacgc gtcgaaggca cgcgtgaagt tgatcgcgac
      781 ggacggggtg ttctcgatgg acggcgtgat cgcgcgcctc aaggacatct gcaacctggc
      841 ggaccgctac ggcgcgctcg tcatggtcga cgactcccac gcgaccggct tcgtcggccg
      901 cggcggccgc ggtacgcccg agcaccgcga ggtcatgggc cggatcgaca tcctcaccgg
      961 cacgctcggc aaggcgctgg gcggggccgc gggcggctat acggcagcgc ggcgcgagat
     1021 cgtcgcctgg ttgcgccagc gttcgcgccc gtacctgttc tccaacagca ttccgccggt
     1081 cgtcgcggcg accacgttgc gcgtgcttga cctcgtcgaa tcgagcgacg acctgcggtc
     1141 ccgcttgcgc gacaacaccg cgtacttccg ccggcgcatg acggaatccg gatttaaggt
     1201 cgtggcgggc gaacacccga tcgcgccggt gatgctcggc gacgcggcac ttgccggcag
     1261 attcgccgaa aagctgctcg accgcggcgt ctacgtgatc ggcttctcgt atccggtcgt
     1321 gccgatgggc aaggcccgca tccgcacgca gatgagcgca ggccacgacc gcgcccagct
     1381 cgaccgtgcc atcgacgcct tcgtcgcggt cggccgcgaa ctcgacgtcc tcgcctgagc
     1441 ctgcgtccca ctgaccacta accacgagcc actagccact tcccatgaag gccctcgtca
     1501 agtcccagcg cgcgcccggc atctggatgg aaaacgtcgc agagccgaag gtcggcccca
     1561 acgacgtgat gatcaaggtg cgcaagacgg cgatctgcgg caccgacatc cacatctaca
     1621 actgggacca gtgggcgcag aagaccgtcc cggtgcccat gcacgtgggc cacgaatacg
     1681 tgggcgagat cgtcgagatc ggcagcgagg tgcagggttt cgcgatcggc gaccgcgtct
     1741 cgggcgaagg tcacatcacc tgcggctact gccgcaactg ccgcgccggc cgtcgtcacc
     1801 tgtgccgcaa cacggttggc gtcggcgtga accgcccggg ctgtttcgcc gagtatctcg
     1861 tgattccggc cttcaacgcc ttcaagctgc cggcctcgat cacggacgaa gtggcatcga
     1921 tcctcgaccc gttcggcaac gccacgcaca cggcgctctc gttcagcctg gtcggcgagg
     1981 acgtgctgat caccggcgcc ggcccgatcg gcgtcatggc ggttgcgatc gcccggcacg
     2041 tcggcgcccg gcacgtcgtg atcaccgacg tcaacgacta ccggctcgac ctggcccgca
     2101 agatgggcgc gacgcgcgcg atcaacgtgt cgcaccagtc gctcgacgac acgatgaagg
     2161 acctcggcat ggtcgagggc ttcgacgtcg gcctcgagat gtcgggcaac ccgagcgcgt
     2221 tccgcgacct gctgcgcacg atgcaccacg gcgggaacgt cgcgctgctc ggcatcccgc
     2281 cggacgacac cgcgatcgac tggaaccagg tgatcttcaa gggcctggtg ctgaagggca
     2341 tctacggccg ggagatgttc gagacctggt acaagatggc cgcgatgctg cagagcggcc
     2401 tcgacatctc gccgatcatc acgcaccgcc tgcccgtcgc ggagttccgc gacggcttcg
     2461 agatcatgcg cagcggccag tccgggaagg tgatcctgga ttgggccgcg taagaaagtg
     2521 gtcagtggcc agtggtcagt ggtcagtggt cagtggtcgg tcgcaccaaa gacgcgctgc
     2581 tcaccagccc ccagccccca gccactagcc actagccact agccactagc cactggattc
     2641 ttacggaggg aaacgtcatg gcaattcgaa gggccgaggc cgagtggacc gggagcctcc
     2701 gcgagggcaa gggcgaactc gcgctcgaga gcggcgcgtt tcgcgggccc tacacgttca
     2761 agtcccggtt cgaggaaggc aaggaaacga accccgagga gctgatcgcg gcggcccacg
     2821 ccggctgctt cacgatggcc ctgacggcgc tgctcgcgcg gcagcagatc gcgcccacgc
     2881 gcatacacac gacggcagcg gtgcacatcg agcagtcggc gggagggttt gcgatcccga
     2941 agatcgaact gcgcacgcgc gcctcgatcc ccggcatgac ggccgagaaa ttcgcggcgc
     3001 tcgccgcgga tgccaagcag aactgcccc
//
LOCUS       DASPFR010000312         4810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1065865, whole genome shotgun sequence.
ACCESSION   DASPFR010000312 DASPFR010000000
VERSION     DASPFR010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065865"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..348)
                     /locus_tag="VD737_08140"
     CDS             complement(52..348)
                     /locus_tag="VD737_08140"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HXW10568.1"
                     /translation="MTDALKPLFAGLTPGLDALARKARSAGSLTEKVRQELAEALRPH
                     LISAVRRGDDLVVIMDSAAWTPRVRYAARALKSRLEAGGEPSIGRLRVKVRGSG"
     gene            502..1416
                     /locus_tag="VD737_08145"
     CDS             502..1416
                     /locus_tag="VD737_08145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXW10569.1"
                     /translation="MNLRITTGRAGRSREIELSGARTTFAAVLLIAAIFAAGLVLGSF
                     LLGAGAEQRAFVRAVAQQRIDLQAARGQIDGQVDALAARVGMLNAQLIRLNALGRRLT
                     DLSGLDRGEFDFDEAPPVGGPEASGEVHEDGSAQVPELAAALDTLEAQIHDREQQLTV
                     LETLLASRELGARSMPGGLPLIGGWISSHFGRRTDPFTGHGAFHAGVDFAGAPGSKVI
                     SVGHGVVSFAGYKSGYGNVVEITHPTGYMTRYGHNSRNLVRTGQSVQKGDPVAVIGST
                     GRSTGTHVHFEVLRDGKVVNPKKYLATP"
     gene            1614..4439
                     /gene="secA"
                     /locus_tag="VD737_08150"
     CDS             1614..4439
                     /gene="secA"
                     /locus_tag="VD737_08150"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HXW10570.1"
                     /translation="MANWLTRIFGSRNQRLVSQYNGIVRKINGYEQALQALSDADLAA
                     KTVEFREQLTAGKTIDDLLPEAFAVVREASRRTLGLRHFDVQLIGGMVLHAGNIAEMR
                     TGEGKTLVATLPAYLNALPGKGAHIVTVNEYLAQRDADWMGPVYNFLGLSVGVVKSGQ
                     TSEEKRAAYNSDITYGTNNEFGFDYLRDNLAFRLEDRMQRELTYAVVDEVDSILIDEA
                     RTPLIISGPAEESTELYLKINELVPRLSRQESEEGEGDFWADEKSKQVHLSDQGHEHV
                     EELFLQAGLLQEGQSLYDPANIRLMHHLNAALRAHALYRRDVEYIVRNGEVIIVDEFT
                     GRTMPGRRWSDGLHQAVEAKENVRVREENQTVASITFQNYFRMYGKLSGMTGTADTEA
                     YEFQQIYGLEVVVIPTHRPMIRDDAPDLVYLTQKDKYDAIIEDIREAVKKGQPSLVGT
                     TSVETSELLSGLLQKEGIEHNVLNAKQHEREAHIVAQAGRPRTVTIATNMAGRGTDIV
                     LGGNLKAELAAIPPEDAAAREAAEAEWKRRHEEVVASGGLHIVGTERHESRRIDNQLR
                     GRSGRQGDPGSSRFFLSLEDNLMRIFGDPERTKRWLQTAGMKEGEVIESKMLSRQIER
                     AQRKVEAYNFDLRKNLLEYDDVANDQRKVIYQQRTELMGAEDISDSVSGIRAEVVNNL
                     IDPFVPRESPEEEWNLDGLREAIARDFLVEIDPKAWLDENDELAEAGLRERVVQAVED
                     AYTAKVESVGAPIMRHLEKAVMLQELDRHWREHLAAMDYLRQGIHLRAYAQKNPKQEY
                     KREAFELFGALLDRIKHDTVSLLSRLQVRTEAEIEAQERERERQLARRLQMQHAAAQS
                     VLSSEPEGAGVAVAETSGGGSGGPRPAFAGGRPGAGAGPTSGPLASGPSEQPVVRDGR
                     KVGRNEPCPCGSGKKYKHCHGQLAREA"
     gene            4493..>4810
                     /locus_tag="VD737_08155"
     CDS             4493..>4810
                     /locus_tag="VD737_08155"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HXW10571.1"
                     /translation="MADRIHVVAGALYDAQGRVLITQRPAGKVLAGRWEFPGGKLHDA
                     EEPYAGLVRELREELDLEVHSAEPLLRYDVEYPDRTIRLDTWLVTSWTGTVRGLDGQA
                     WKWV"
BASE COUNT          825 a         1672 c         1624 g          689 t
ORIGIN      
        1 ccagctcgac gctggccctc ttcgtcttca ctagccactg accactagcc actaaccact
       61 tcctcgcacc ttcacccgca gtcgcccgat cgaaggctcc ccaccggcct cgaggcgcga
      121 cttgagcgcg cgggccgcgt agcgcacgcg cggcgtccag gccgcggaat ccatgatcac
      181 gaccaggtcg tcgccgcgac ggacggcgga aatcaggtgc gggcgcagcg cttcggccaa
      241 ttcctgacgg actttctcgg tgagcgaccc ggcggagcgc gccttgcggg ccagggcgtc
      301 gagcccgggg gtgaggcccg cgaacagggg cttgagtgcg tccgtcattg tcaacagatt
      361 tttgagaaaa gcgcttatta tttgccaagt gcgtggccgc tgccccatag tctgccgatt
      421 ccgtgacacg acgcaacgtt gcccgcgggc acgtccggcg accgtgttcc tttcgcctct
      481 ccaacgcggg accggcgtga catgaatttg cgtatcacca ccggccgtgc gggccgcagc
      541 cgggagatcg aactctcggg cgcgcgcacc acgttcgcgg ccgtactgct catcgccgcg
      601 atctttgcgg ccggcctcgt gctgggaagc ttcctgctcg gagcgggcgc ggagcagcgc
      661 gcgttcgtcc gggccgtggc gcagcagcga atcgacctgc aggcggcgcg cggtcagatc
      721 gacggccagg tcgacgcctt ggcggcccgc gtggggatgc tcaatgcgca actgatccgc
      781 ctgaatgcgc tggggcggcg gctgaccgac ctgtccggcc tcgaccgcgg ggaattcgat
      841 ttcgacgagg cgccccccgt cggcggcccg gaggccagcg gcgaggtgca cgaggacggt
      901 tcggcacagg tgcctgagct cgcggcggcg ctcgacaccc tggaggccca gatccatgac
      961 cgtgagcagc agctgaccgt gctcgagacg ctgctcgcct cgcgcgaact cggcgcgcgc
     1021 agcatgccgg gcggcctgcc actgatcggc ggctggatct cctcgcattt cggccgtcgc
     1081 accgacccgt tcaccggcca cggcgcgttc cacgcgggcg tcgacttcgc gggggcaccg
     1141 ggcagcaagg tcatctcggt cggacacggc gtcgtgtcgt tcgcgggtta caagtcgggc
     1201 tacggcaacg tcgtcgagat cacgcacccc acgggctaca tgacgcggta cggccacaat
     1261 tcgcgcaacc tggtgcgcac cggccagtcg gtccagaagg gagacccggt tgccgtcatc
     1321 ggcagcaccg gacgttccac tggcacgcac gtgcacttcg aggtgctccg ggacggcaag
     1381 gtggtcaacc cgaagaagta cctggcgacg ccgtagcgga cggcctccgt cggactgcgc
     1441 cgcggtccgc acgtccttga cgcgttgaac ctccgctccg gtacgtctgc tgccagcgcc
     1501 ggcgtccgac cacccgcact ttccgtaacc tcttgtttct acagcgcccc gcccccactt
     1561 cggtgatcct gtagaatttc cgcctttcac ctcccgtctc gcccggagac cctgtggcca
     1621 attggctcac ccgcatcttt ggcagccgca accagcggct cgtcagccag tacaacggca
     1681 tcgtccgcaa gatcaacggc tacgaacagg cgctgcaggc cctctcggac gccgacctcg
     1741 ccgccaagac cgtcgagttc cgcgagcagc tcaccgccgg caagaccatc gacgacctgc
     1801 ttccggaggc gttcgcggtg gtccgtgagg cctcgcgccg cacgctcggc ctgcgccact
     1861 tcgacgtgca gctgatcggc ggcatggtgc tgcacgccgg caacatcgcc gagatgcgca
     1921 ccggcgaggg caagacgctc gtcgcgacgc tgccggcgta cctgaacgcg ctgcccggca
     1981 aaggcgcgca catcgtcacc gtcaacgagt acctcgcgca gcgtgacgcc gactggatgg
     2041 ggccggtcta caacttcctc ggcctgagcg tcggcgtggt gaagtccggc cagacctcgg
     2101 aggagaagcg cgccgcgtac aactccgaca ttacctacgg caccaacaac gagttcggct
     2161 tcgactacct gcgcgacaac ctcgcgttcc ggctcgagga ccgcatgcag cgcgagctca
     2221 cgtacgcggt cgtcgacgag gtcgactcga tcctgatcga cgaggcgcgc acgccgctga
     2281 tcatctccgg ccccgcggag gagagcaccg agctgtacct caagatcaac gagctcgtgc
     2341 cgcggctgtc gcggcaggag tccgaggaag gcgagggcga cttctgggcg gacgagaagt
     2401 ccaagcaggt gcatctctcc gaccagggcc acgagcacgt cgaggagctg ttcctgcagg
     2461 ccggactgct gcaggaaggg cagagcctct acgaccccgc caacatccgg ctgatgcacc
     2521 acctgaacgc ggcgctgcgc gcgcacgcgc tctatcgccg cgacgtcgag tacatcgtgc
     2581 gcaacggcga ggtcatcatc gtcgacgagt tcaccggccg cacgatgccg ggacgtcgct
     2641 ggtccgacgg cctgcaccag gcggtcgagg ccaaggaaaa cgtgcgcgtg cgcgaggaga
     2701 accagaccgt cgcctcgatc acgttccaga actacttccg catgtacggc aagctctcgg
     2761 gcatgaccgg caccgcggac accgaggcct acgagttcca gcagatctac gggctcgagg
     2821 tcgtcgtgat cccgacacac cgcccgatga tccgcgacga cgcgcccgac ctcgtctacc
     2881 tgacgcagaa ggacaagtac gacgcgatca tcgaggacat ccgcgaggcc gtgaagaagg
     2941 gccagccttc gctggtcggc acgacctcgg tcgagacctc cgagctgctc tcgggcctgc
     3001 tgcagaagga aggcatcgag cacaacgtgc tgaacgccaa gcagcacgag cgcgaggctc
     3061 acatcgtcgc gcaggccggg cgtccgcgca ccgtgacgat cgcgaccaac atggccggcc
     3121 gcggcaccga catcgtgctc ggcggcaacc tgaaggccga gctcgcggcg attccgcccg
     3181 aggacgctgc ggcgcgcgaa gcggccgaag ccgagtggaa gcggcgtcac gaggaagtcg
     3241 tcgcctcggg cggcctgcac atcgtcggca ccgagcgcca cgagtcccgc cgcatcgaca
     3301 accagttgcg cggccggtcc ggccgccagg gcgacccggg ctcgagccgg ttcttcctgt
     3361 cgctcgaaga caacctgatg cggatcttcg gcgacccgga gcgcaccaag cgctggctgc
     3421 agaccgccgg catgaaggaa ggcgaggtga tcgagagcaa gatgctctcg cgccagatcg
     3481 agcgcgcgca gcgcaaggtg gaggcgtaca acttcgacct gcgcaagaac ctgctcgagt
     3541 acgacgacgt cgccaacgac cagcgcaagg tcatctacca gcagcgcacg gagctgatgg
     3601 gcgcggagga catctccgat tccgtttccg gcatccgggc cgaggtcgtc aacaacctga
     3661 tcgacccgtt cgtgccgcgc gagagccccg aggaggagtg gaacctcgac ggcctgcgcg
     3721 aggccatcgc ccgcgacttc ctggtcgaga tcgacccgaa ggcgtggctc gacgagaacg
     3781 acgagctcgc ggaggccggc ctgcgcgagc gtgtcgtgca ggcggtcgag gacgcgtaca
     3841 ccgccaaggt cgagtccgtc ggcgcgccga tcatgcgcca cctcgaaaag gccgtgatgc
     3901 tgcaggagct cgaccgccac tggcgcgagc acctcgccgc gatggactac ctgcggcagg
     3961 gcatccactt gcgcgcctac gcgcagaaga acccgaagca ggagtacaag cgcgaggcct
     4021 tcgagctgtt cggggcgctg ctcgaccgca tcaagcacga caccgtgtcg ctgttgtcga
     4081 ggctgcaggt gcgcaccgag gccgaaatcg aggcgcagga gcgcgagcgc gagcgccagc
     4141 tcgcgcggcg gctgcagatg cagcacgccg cggcacaatc cgtgctctcg agcgaacccg
     4201 aaggtgccgg tgttgcggtt gcggaaactt cgggcggtgg ctccgggggc ccgcgaccgg
     4261 ccttcgccgg cggtcgaccg ggggcgggtg ctggtccgac cagcggtccg ctcgcgtcgg
     4321 gcccgtcgga gcagcccgtc gtgcgcgacg ggcgcaaggt cggtcgcaac gagccctgcc
     4381 cctgcggctc gggcaagaag tacaagcact gccacggcca gctcgcgcgc gaagcgtgag
     4441 gcacctctcc cgcggggcgg gaggggtcgc gcgcagcgcg ggtgagggct cattggccga
     4501 tcgcatccac gtcgtcgccg gcgcgctcta cgacgcgcaa ggccgcgtgc tgatcacgca
     4561 acgtccggcc ggcaaggtgc tcgcgggacg ctgggaattc ccgggcggca agctgcacga
     4621 cgccgaagag ccgtacgcag gcctcgtgcg tgagctgcgc gaggagctcg acctcgaggt
     4681 ccactccgcc gagccgctgc tgcgttacga cgtcgaatac cccgatcgca cgatcaggct
     4741 cgacacgtgg ctcgtgacgt cgtggacggg cacggtgcga ggcctcgacg ggcaggcgtg
     4801 gaagtgggtc
//
LOCUS       DASPFR010000313         7643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1431713, whole genome shotgun sequence.
ACCESSION   DASPFR010000313 DASPFR010000000
VERSION     DASPFR010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7643
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431713"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..819
                     /locus_tag="VD737_08160"
     CDS             202..819
                     /locus_tag="VD737_08160"
                     /inference="COORDINATES: protein motif:HMM:NF017415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HXW10572.1"
                     /translation="MANRDTSSNRQRARKRAGGRAPSSVPPRARARTRDDAEAAQDIQ
                     AEWLQGVQQVWLAGMGALARAQKDGPGAFTDAVVEGLKLLGQSRSAAQRLMRGVFETA
                     QAGVQSRVGGARSQAQETWDNLESLFQSRVQRAMHQVGVPTADEIRVLTRRVAELNEN
                     VKQLNARERLRRTATAHTRATGKPARKTARKSARKTTGKAPAADE"
     gene            857..2104
                     /locus_tag="VD737_08165"
     CDS             857..2104
                     /locus_tag="VD737_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXW10573.1"
                     /translation="MLDLQTAARTLRQRPSHRRFGLALAGGGPLGAFYEIGCLHAIDE
                     ALDGFGLTELEMYVGVSSGAMIAAGLANGFDTADMGLVFIHAESNEFSFSPAWFLMPA
                     VGEYVRRLALAPGLFAGIAREYLRDPGRNWAEAIDPLGRLLPTGVCDNRPLERVLARL
                     FSTGGRTNDFRRLAPRLYVMATELGTGHSVRFGAQGYDHVPISRAIQASTALPGLYPP
                     VTIDGHVYADGALVKTMHASVLLDAGADFVICVNPLVAFDASSAPREPDGYAPPEHEL
                     TTGGLPTVLSQTFRSLIQSRMRVGMRDYRQRYPRADVLLFEPDRGDTRLFFQNVFRYA
                     DREDLAEHAYQCTRADLRHHAHALQAMLDRHEIRLRLDVLHDRTRTFAHAVDLRKRRQ
                     LPLVGPLHLALDRLEHIVATGRA"
     gene            2107..2880
                     /locus_tag="VD737_08170"
     CDS             2107..2880
                     /locus_tag="VD737_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10574.1"
                     /translation="MKLLGIEIGAAPSERGRLGRLLERNAALRDVFASDPKLARRLAR
                     LQKWQSKRLLRSHADLHGNPRYRRAVEFFFRELYGAGDARRRDDDLIKVQTAMERLLP
                     REGLEALCLAIELETLSQELDADVARNLPDAPITVERYAEAYREAGREADRRRQIDLT
                     AKVGSYLDGVVRKPIVGALVRLARAPAHAAGFGRLQEFLEHGLTAFETMGGAAEFLET
                     IRARELEASARLYKGDPDPFEFDAGSNARRSRRPKAPIG"
     gene            2877..3548
                     /locus_tag="VD737_08175"
     CDS             2877..3548
                     /locus_tag="VD737_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10575.1"
                     /translation="MSSPGKTSQDSRKTRSRKPPPRWRRYLVQAELELPPPSSHASTG
                     VRTPTGDDAPEGDPDAPPWYQTRFAVLALAASCLALAVVTSIALWNGARDRETANAAE
                     ALARALTLRAVNSERTLRIAPNPRSWSATPDASIAWPEPPQLLELHLPVAYARYAAYA
                     LVVDKVDQGRVMVIERMTADSNRDLRLAVNSSAFGPGEYRLRVQGYTWKGQRVDVGWV
                     RLVVR"
     gene            complement(3555..6038)
                     /locus_tag="VD737_08180"
     CDS             complement(3555..6038)
                     /locus_tag="VD737_08180"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXW10576.1"
                     /translation="MTWLTLALRNLLRDLRSGEIAVLVLALLVAVASLTAVGFFTSRI
                     GRAVEQQAGEVLAADLRLRSARPLDEGYDREARRRGLRVARVEIMPSVVFFGEESSLV
                     TLRAVGTGYPLRGRVKTADRPFGPGVATDDVPAPGEAWADSRLLAKLGADVGARLAVG
                     ATSVRVSRVLDYRPDQGSGFADLSATLMINLADLAATELVQPGSRVSRAVLFAGEPAA
                     VEQFTEWLEANKRTGERLQSIADASPQIRASADRAGRFLSLASLVSVLLSAIAVAMAA
                     QRYARRHLDTVALMKCMGASQRLVQLHTVSQLVVIGVVTAVVGTAVGYFAQTGIAWLL
                     RDLLRGELPPPSLDAFWLGVVTAVVVLVGFALPPLLQLRRVPPGRVLRRDLEPPPLRY
                     AVVYGLAIGAVLALLYWIVRDPRLVGYVAGGLGATFVLLALAGWLLVRMLGPLRRGVG
                     IAWRYGLANIVRRGRDSIVQIVAFGLGFTVLLLLGLVRDDLLRDWRASLPADAPNYFM
                     INIRPDEGERVRAFFDRAGFPSTDLVPMLRARLTGIDGRSVDALQFASDRASEFVERE
                     ANLTWSATPGPGNTLVAGRWWPASGPREPEVSVEVEYAETLGLELGETLTYDIAGETV
                     TARITSLREVRWDTFKPNFFVVFSPGVLDDVTGTLITSVHVRPDQRPALVELVRQFPE
                     VTIIDIEAMLAQVRDVMDKASLAVQYVFLFTLAAGLMVLLAAVQATRDERRYESAMLR
                     TLGATRSVVFQGVAAEFLVLGALAGLLAAAGATGVGWLLARRVFDLDYSPDPWIWLIG
                     VLGGALLVGIAGLLAARSVVSHPPMQALR"
     gene            complement(6047..6772)
                     /locus_tag="VD737_08185"
     CDS             complement(6047..6772)
                     /locus_tag="VD737_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003189864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXW10577.1"
                     /translation="MTSHDGTRLAALAADRDETVLAAENIGKEVSSPEGTLAILAGVS
                     VAIRRGESVAVVGASGAGKSTLLALLAGLDEPTTGRAWLAGHDLTALDEDGRAAVRAR
                     HVGFVFQSFHLLPSLTALENVMLPLELARRPDARAAAKDVLARVGLAGRLGHYPRQLS
                     GGEQQRVAIARAFVTQPDVLFADEPTGNLDAATGERIMDLLFGLNGATRTTLVLVTHD
                     RALAARCDRVIRLDAGRVLAPAA"
     gene            6792..7391
                     /locus_tag="VD737_08190"
     CDS             6792..7391
                     /locus_tag="VD737_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HXW10578.1"
                     /translation="MPFSAVARAADGASPPAILVLGDSLSAAFGIRVEQGWVSLLQAR
                     LRAKGYGHRVVNASSSGETTGGALARLPRALATHRPAVVVVELGGNDGLRGLPVADIR
                     SNLEAIVQRSRQSGAKVLLVGMRIPPNYGGPYTKQFHELFGELAQEHRLPLVPFFLER
                     VALDDSLMQDDGIHPTAAAQPILLDEVWPRLEPLLRSSR"
BASE COUNT         1193 a         2760 c         2665 g         1025 t
ORIGIN      
        1 cagcgccacc gcggacgact gcaacggtag cggcagaacc ggcgtcgcag ccactccctg
       61 cagccggcgt cgtgcaaggg tcgagcgcaa gggcgtcagg cgatgccttt gcgggttgta
      121 cctaggcctc ggattgacac actgcagcaa gcccttagca tcggccagaa tagtgagggc
      181 cgaggcgccg ggagttccgt aatggcgaac cgcgacacga gcagtaatcg gcagcgcgcg
      241 cgcaagcgag ccggcggtcg cgctccatcg agcgtgccgc cgcgtgcgcg ggcccgcacc
      301 cgcgacgacg ccgaagcggc gcaggatatc caggctgagt ggttgcaggg cgtgcagcag
      361 gtctggcttg ccggcatggg cgccctcgcg cgggcacaga aggacggccc tggcgccttc
      421 accgacgccg tcgtcgaagg gctcaagctg ttgggtcagt cacgctccgc ggcccagcgc
      481 ctgatgcgcg gcgtgttcga gaccgcgcag gcgggcgtgc agtcgcgcgt gggcggcgca
      541 cggagccagg cgcaagagac gtgggacaac ctcgagtcgc tgttccagag ccgcgtgcag
      601 cgggcgatgc accaggtcgg cgtgccgacc gcagacgaga tccgcgtcct gacgcggcgc
      661 gtcgccgagc tcaacgagaa cgtgaagcag ctcaacgccc gcgagcgtct gcgccgcacg
      721 gcgaccgcgc acacccgcgc cacgggcaag cctgcgcgca agaccgcacg caagagtgca
      781 cgcaagacca ccggcaaggc gccggccgcc gacgaatgaa ggaccgccgg acgcactgac
      841 gccggctgga accagcatgc tcgacctgca gaccgccgcc cggacccttc gccagcgccc
      901 cagtcatcgt cgtttcggcc tcgcgctcgc aggcggcgga ccgctcggcg cgttctacga
      961 gatcggttgc ctgcacgcga tcgacgaggc gctggacggc ttcggactca ccgaactcga
     1021 gatgtacgtc ggcgtcagct cgggggccat gatcgccgcg ggcctggcca acggcttcga
     1081 cacggccgac atgggcctgg tcttcatcca cgccgagtcg aacgagttca gtttctcgcc
     1141 cgcgtggttc ctgatgccgg ccgtcggcga gtacgtgcga cggctggcgc tcgcgcccgg
     1201 actctttgcc ggcatcgcgc gcgagtacct tcgcgatcct ggtcgcaact gggccgaggc
     1261 catcgatccg ctcggccgcc tgctgccgac cggcgtctgc gacaaccgac cgctcgagcg
     1321 agtgctggcg cgcctgttct ccacgggcgg acgcaccaac gattttcgcc ggctcgcccc
     1381 gcgcctctac gtcatggcga ccgaactcgg cacggggcat tcggtgcggt tcggcgcgca
     1441 ggggtacgac cacgtaccga tctcgcgcgc gatccaggcg agcaccgcgc tgccgggcct
     1501 gtatccgccg gtgacgatcg acgggcacgt ctacgccgac ggcgcgctcg tcaagacgat
     1561 gcacgcgtcg gtgctgctcg atgcgggggc cgacttcgtg atctgcgtga atccgctggt
     1621 cgccttcgac gcctcgagtg cgcctcgcga accggacgga tacgcgccgc cggagcacga
     1681 actcacgacc ggcgggctgc cgacggtcct gagccagacg ttccgctcgc tgatccagtc
     1741 gcggatgcgc gtcggcatgc gcgactaccg gcagcgctac ccgcgggccg acgtcctgct
     1801 gttcgaaccc gaccgcggcg acacgcggct gttcttccag aacgtgttcc gctacgcgga
     1861 ccgcgaggac ctggccgagc acgcctacca gtgcacgcgc gcggacctgc gccaccacgc
     1921 ccacgccctg caagcgatgc tggaccggca cgagatccgg ttgcgactgg acgtgctgca
     1981 cgaccgcacg cgcacgttcg cgcacgcagt cgacctgcgc aagcgtcgcc agctgccgct
     2041 cgtcgggccg ctgcatctcg cgctcgaccg gctcgagcac atcgtcgcga ccggtcgggc
     2101 ctgacggtga agctgctcgg catcgagatc ggcgcggcgc cgtccgagcg cggcaggctc
     2161 gggcggctgc tcgagcgcaa cgccgcgctg cgcgacgtgt ttgcgtccga ccccaaactc
     2221 gcccggcgcc tggcccgcct gcagaagtgg cagtcgaagc ggctgctgcg ctcgcacgcg
     2281 gacctgcacg ggaatccgcg gtatcggcgt gcggtcgagt tcttcttccg cgagctctat
     2341 ggcgccggcg atgcgcggcg tcgcgacgac gacctcatca aggtgcagac cgcgatggaa
     2401 cggttgctgc cgcgcgaagg gctcgaggca ctgtgcctgg ccatcgaact cgaaaccttg
     2461 tcgcaggaac tcgacgcgga cgtcgcgcgc aacctgcccg acgcgccgat cacggtcgag
     2521 cgctatgccg aggcctatcg cgaggccggg cgcgaggccg accgtcgccg gcagatcgac
     2581 ctgacggcga aggtcggctc ctacctggac ggcgtggttc gcaaaccgat cgtcggggcg
     2641 ctggtcaggc tggcgcgggc acccgctcac gcggcaggat tcggccggct gcaggagttc
     2701 ctcgaacacg gcctcacggc tttcgaaacg atgggcggtg ccgcagaatt cctcgagacg
     2761 attcgcgcgc gtgagctgga ggcctcggcc cgcctgtaca agggcgatcc ggaccccttc
     2821 gagttcgacg ccggcagcaa cgcccgaaga tcacgcaggc ccaaggcacc gatcggatga
     2881 gctcacccgg caagacgagc caggactccc gcaagaccag atcccgcaag ccgccgcctc
     2941 gttggcgccg ctacctcgtg caggcagagc tcgagctgcc gccgccttcg tcgcacgctt
     3001 cgacgggtgt gcggacgccg acgggcgatg acgcgccgga gggggatccg gacgcgccgc
     3061 cgtggtatca gacgcgattc gccgtgctcg ccctcgccgc gagctgtctc gcgctggccg
     3121 tggtgacctc gattgcgctc tggaacggcg cgcgcgaccg ggagaccgcg aacgccgccg
     3181 aggccctggc ccgcgcgctc acgctgcgcg cggtcaacag cgagcggaca ctgcgcatcg
     3241 cgcccaaccc ccgcagctgg tcagccaccc cggacgcctc gatcgcctgg ccggagccgc
     3301 cacagctgct cgagctccat ctgccggttg cctatgcgcg gtacgcggcg tacgcgctcg
     3361 tcgtggacaa ggtggatcag ggccgcgtca tggtgatcga gcggatgacg gcggattcga
     3421 atcgcgacct gcggctcgcg gtcaacagct cggccttcgg tcccggcgag taccggctcc
     3481 gcgtgcaggg ctacacgtgg aaaggccagc gcgtggacgt cggctgggtg cggctggtgg
     3541 tgcgataact gccttcagcg cagcgcctgc atcggcgggt gcgacaccac cgatcgggcg
     3601 gccagcagtc cggcgatgcc gaccagcaag gccccgccca gcacgccgat cagccagatc
     3661 caaggatccg gcgagtagtc caggtcgaac acgcgacgcg cgagcagcca gccgacgccg
     3721 gtcgcgcccg cggcggcgag caggccggcg agcgcgccga gcaccaggaa ctcggccgcc
     3781 acgccctgga acacgacgct gcgcgtcgcg cccagcgtcc gcagcatcgc gctttcgtag
     3841 cgacgctcgt cgcgcgtcgc ctgcacggcc gccagcaaca ccatcagccc cgcggcgagc
     3901 gtgaacagga acacgtactg gaccgcgagg ctcgccttgt ccatgacgtc gcggacctgg
     3961 gccagcatcg cctcgatgtc gatgatcgtg acctcgggaa actgccgcac gagctcgacc
     4021 agggcaggcc gctggtcggg tcgcacgtgc acgctcgtga tcagcgtgcc ggtcacgtcg
     4081 tcgagcacgc cgggggagaa cacgacgaag aaattgggct tgaacgtatc ccagcgcacc
     4141 tctcgcaggc tcgtgatccg cgcggtgacg gtttcgcccg cgatgtcgta ggtgagcgtc
     4201 tcgccgagct cgagccccag cgtttcggcg tactcgacct cgaccgacac ctcgggctcg
     4261 cgtggtccgc tggctggcca ccagcgaccg gctacgagcg tgttgcccgg cccgggcgtc
     4321 gcggaccagg tcaggttggc ctcgcgctcg acgaactcgc tggcgcggtc ggatgcgaac
     4381 tgcagcgcgt cgacggaccg gccgtcgatc cccgtcagcc gcgcccgcag catcggcacc
     4441 aggtccgtcg acggaaatcc cgcgcgatcg aagaacgccc gcacccgctc gccctcgtcg
     4501 ggacggatgt tgatcatgaa gtagttgggc gcgtccgccg gcaggctggc gcgccagtcg
     4561 cgcaacaggt cgtcccgcac cagtccgagc agcagcagga ccgtgaagcc cagcccgaat
     4621 gcgacgatct gcacgatgct gtcacgaccg cggcgcacga tgttggcgag gccgtagcgc
     4681 caggcgatgc cgacgccgcg gcgcagcggc ccgagcatgc gcaccagcag ccaccccgcc
     4741 agcgccagca gcacgaacgt ggcgccgagc ccgcccgcga cgtagccgac cagtcgaggg
     4801 tcccgcacga tccagtacag cagcgcgaga acggcgccga tcgccagccc gtacacgacc
     4861 gcgtatcgca gtggcggcgg ctccaggtcg cgtcgcagca ctcgtcccgg cggcacgcga
     4921 cgcagctgca gcagcggcgg cagcgcgaag ccgaccagca cgacgaccgc ggtcacgacg
     4981 ccgagccaga acgcatcgag cgagggtggc ggcagctcgc cgcgcaggag gtcgcgcagc
     5041 agccaggcga tgcccgtctg tgcgaagtaa ccgacggcgg tgccgacgac cgccgtcacg
     5101 acgccgatca cgaccagctg cgagacggtg tgcagctgga cgagccgctg cgaggccccc
     5161 atgcatttca tcagcgccac ggtgtcgaga tgacgtcgcg cgtagcgctg ggccgccatc
     5221 gccacggcaa tcgcggaaag cagcacgctg accaggcttg cgagcgacag gaagcgcccg
     5281 gcgcgatccg ccgacgcgcg aatctgcggg ctcgcgtccg cgatcgactg cagccgctcg
     5341 cccgtgcgct tgttcgcctc gagccactcc gtgaactgct cgacggcggc cggctctccc
     5401 gcgaacagca cggcgcggga cacgcgactg cctggttgaa cgagttccgt cgccgccagg
     5461 tcggccaggt tgatcatcag ggttgcggac aggtcggcga atccgctgcc ctggtccgga
     5521 cggtaatcga ggaccctgct cacgcggacg ctggtcgcgc cgaccgcgag gcgcgcaccg
     5581 acgtcggcgc cgagcttggc gagcaggcgc gaatcggccc aggcctcgcc tggtgcgggc
     5641 acatcgtcgg tggcgacccc cgggccgaag ggccggtctg ccgtcttcac gcgtccccgg
     5701 agcggatagc ctgttcccac ggcccgcagc gtgaccagcg aggactcctc gccgaagaac
     5761 acgacgctcg gcatgatctc gacgcgggcc acgcgcaggc cacgacgccg ggcctcgcgg
     5821 tcgtaaccct cgtcgagcgg ccgggccgag cgcaaccgca ggtcggccgc cagcacctcg
     5881 ccggcctgct gctcgacggc gcgcccgatg cggctcgtga agaagccgac ggcggtcagc
     5941 gatgccacgg cgacgagcag cgcgagcacg agcaccgcga tctcgccgga gcgcaggtcg
     6001 cgcagcaggt tgcgcagcgc cagcgtgagc caggtcacga ccgcgctcag gccgccggcg
     6061 cgagcacgcg gcctgcgtcg agccggatca cccggtcgca gcgcgccgcg agcgcacggt
     6121 cgtgggtcac cagcacgagc gtggtccgcg tcgcaccgtt gagcccgaac agcaggtcca
     6181 tgatgcgttc gccggtcgcg gcatcgaggt tgccggtcgg ctcgtccgcg aacaggacgt
     6241 cgggctgcgt gacgaacgcc cgcgcgatcg cgacgcgctg ctgctcgccg cccgacagct
     6301 gccgtggata gtgcccgagc cggccggcga gaccgacccg cgcgagcacg tccttcgcgg
     6361 cagcacgcgc gtccggccgg cgcgcgagct cgagcggcag catcacgttc tccagcgcgg
     6421 tgagcgacgg caggagatgg aacgactgga acacgaagcc cacgtggcgt gcgcgcacgg
     6481 cggcgcggcc gtcttcgtcg agcgccgtca ggtcgtgacc ggccagccag gcgcgcccgg
     6541 tggtcggctc gtcgagcccg gcgagcagcg cgagcaacgt ggacttgccg gcgcccgagg
     6601 cgcccacgac ggccaccgac tcaccacgcc ggatggccac cgacacgccg gccagaatgg
     6661 cgagcgttcc ctcaggactg ctaacctcct tgccgatgtt ctctgccgcc agcaccgttt
     6721 cgtcccggtc agccgcaagc gcggcgagtc gtgttccgtc gtggctcgtc atcctcgtcc
     6781 tctgcctggc agtgccgttt tccgccgtcg cgcgtgcagc cgacggcgcg tcgccacccg
     6841 ccatcctggt gctcggcgac agcctcagcg cggccttcgg gatacgcgtc gagcagggct
     6901 gggtgtccct gctgcaggcc agactgcgcg ccaaaggcta cggacatcgc gtggtcaacg
     6961 ccagttccag cggcgagacg accggcggcg cgctcgcccg cctgccgcgg gcgctcgcga
     7021 cacatcgccc cgcggtggtg gtcgtcgagc tcggcggcaa cgacggcttg cgcggcctgc
     7081 cggtcgcgga catccggtcg aacctcgagg cgatcgtcca gcgttcgcgg cagtcgggcg
     7141 caaaggtgct gctcgtgggc atgcgcatcc cgccgaatta cggcggccct tacacgaagc
     7201 agttccacga gctcttcggg gagctcgcgc aggagcaccg actgccgctc gtgccgttct
     7261 tcctcgagcg tgtcgcgctg gatgattcgt tgatgcagga cgacgggatt cacccgaccg
     7321 cggccgcgca accgatcctg ctcgacgagg tgtggccgcg gctcgagccg ctgctgcggt
     7381 catctcgata atcactcaga cagcgcgcgg cgatggctgc cgtgcccagt cgatggcacg
     7441 acgctcgggc gccagcgcag cgggtgccga tggtcgtggc cagggccgtc gcgcaacagg
     7501 acgttgcgcg cggagcctcc agggacggat ttacggcgtc cctggacacg atctcggcgg
     7561 cggctgcgct ggcgcaatca cggacacccg acgctctcga cgtgtcggtg tattcctcgg
     7621 cgcgctgcta tataacgcga acg
//
LOCUS       DASPFR010000314         8256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_91055, whole genome shotgun sequence.
ACCESSION   DASPFR010000314 DASPFR010000000
VERSION     DASPFR010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8256
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91055"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VD737_08195"
     CDS             complement(<1..189)
                     /locus_tag="VD737_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10579.1"
                     /translation="MDGDPATSTRPRAGTLLPRTVVVLGVVSFLNDAASEMITPLLPL
                     FLTATLGAGPAIVGLVEGV"
     gene            319..819
                     /locus_tag="VD737_08200"
     CDS             319..819
                     /locus_tag="VD737_08200"
                     /inference="COORDINATES: protein motif:HMM:NF023621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HXW10580.1"
                     /translation="MSIFGRILEKLGIGKDDDKNKGRTPPASSTQAQAGRPSVAPTQT
                     TPHTSSPGAQRTAPPPSPQQAQAARPAQAQQPRAMSETDVVAKLEKLAADNPQKLNWR
                     TSIVDLMKLLGMESSLAERKELAAELGYPRDQMDDSAKMNVWLHKQVLRKIAENGGNV
                     PKELLD"
     gene            complement(957..1151)
                     /locus_tag="VD737_08205"
     CDS             complement(957..1151)
                     /locus_tag="VD737_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10581.1"
                     /translation="MTSQGTRPFASCSEPELLEHAIARLAARHGDRGVIADHLRMQLA
                     HLRQTEMQRRLLQARPKPFA"
     gene            complement(1258..1620)
                     /locus_tag="VD737_08210"
     CDS             complement(1258..1620)
                     /locus_tag="VD737_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HXW10582.1"
                     /translation="MAADRSPTLEALAARLAEFACERDWEQFHSPKNLAMALAGEVGE
                     VLEHFQWLTESQSRNLPPDVRDAVALELADVLLYLIRLADQAGIDLGEAAARKLELNA
                     GKYPVERFRGSSRKYDRS"
     gene            complement(1620..2759)
                     /gene="asd"
                     /locus_tag="VD737_08215"
     CDS             complement(1620..2759)
                     /gene="asd"
                     /locus_tag="VD737_08215"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HXW10583.1"
                     /translation="MKTAIVGWRGMVGSVLMQRMAAERDLDHVEPLFFSTSQAGAAAP
                     VVGGRKPSGPLQDAHDIEALAQCDVVVTCQGGDYTNAVHPKLRAAGWQGYWIDAASAL
                     RMKDDAVIILDPVNRDVIDRALAAGIKDYIGGNCTVSLMLMALGGLFRADLIEWVTAM
                     TYQAASGAGAPNMRELVEQMGAVHDCAVSMLRDPASAILDIDQAVTARLRSAELPQAQ
                     FGAPLAGSLIPWIDKDLGNGQSREEWKGSAETNKILGRTGAATIPVEGICVRIGAMRC
                     HSQALTIKLRRDLPLAEIERTLAQGNEWIRLVPNTRDASIAELSPAKVSGTLQIPVGR
                     VRKLAMGGEYVSAFTVGDQLLWGAAEPIRRMLRILVEARRQTAAA"
     gene            complement(2870..5461)
                     /locus_tag="VD737_08220"
     CDS             complement(2870..5461)
                     /locus_tag="VD737_08220"
                     /EC_number="2.7.2.4"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF006515.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/diaminopimelate
                     decarboxylase"
                     /protein_id="HXW10584.1"
                     /translation="MTGHSDSNWVVLKFGGTSVSSVSNWKSVAAVVKERLAEGLHPVI
                     VHSALSGITDRLEQLLAKALTDEWEPVMDQIERRHMDLARDLGVPDVAAELDHHFSEL
                     RRSAEGIYLIGEVSDRLRARVMANGELMATRIGAAFLKHEGVDAQWLDARKLLHAEER
                     PGASERANYLSATCNFEPDADMQQRLGSVGRVLITQGFIASDARGDTVLLGRGGSDTS
                     GSYFAAKLRARRLEIWTDVPGMFTANPRSVSNARLLKSLDYDEAQEIASSGAKVLHPR
                     CILPVKQYAIPLSVHATQQPELAGTVVTATGGDGGARVKAVCIKKGITLLSMETLGMW
                     HQVGFLADAFAVFKQHGLSVDLVSTSETSVTVSLDPAANALDERTLEALMTDLSRLCR
                     VEVIGPCSAVSLVGRSIRATLHRLGDALELFEEQKIYLVTQAANDLNITFVVDEEQGD
                     RLVRRLHDVIVWNAPSDHVLGPTWEQIHGLGPVLPAGGPPWWQDRREELQAIAREHGA
                     AYVYDRASLELAARQLLDIRGVDRIFYAVKANPHPEILRTFAGLGLSFECVSQGECQH
                     VFDTVRGIDPSRILFTPNFAPRAEYEWALEKRVNLTVDSLYALREWGSLFRGRDVLVR
                     LDTGFGRGHHDHVRTAGVHSKFGVPLFELDELERLVRKVGCRVVGLHAHTGSGVFEVR
                     NWQEVGNLLGELAKRFPDARAVDLGGGLGVPEKPGQVPVDLAALQGVIDELRGKYPQL
                     QLWIEPGRFLVAQAGVLLVQVTQLKGKGAVQYVGVATGMNSLIRPALYGAHHDIVNLT
                     RYGEPGTEVYNVVGPICESADVLGYERLLPPTREGDVLLVANAGAYGRAMSSSYNLRE
                     PAREVVV"
     gene            complement(5593..5799)
                     /locus_tag="VD737_08225"
     CDS             complement(5593..5799)
                     /locus_tag="VD737_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10585.1"
                     /translation="MERDEGQFDLDEDFVANAEDGEDYDRFIDRVDRRKQAVKKGKAA
                     WSKLEDVLAEKRLQKELKDYYEDQ"
     gene            complement(5991..6827)
                     /locus_tag="VD737_08230"
     CDS             complement(5991..6827)
                     /locus_tag="VD737_08230"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HXW10586.1"
                     /translation="MPSPDRDHAPFTPSGVITLTTDFGHKDPFVGVMKGQVLRRFPTA
                     QLVDLTHEVLVHWPAEAGFWLGRSYRYFPPGTVHVAVVDPGVGSARDIAIVEADGQVF
                     LAPDNGLLAGVLERVETAATLRHLDLGVLPRLDVHRPSATFHGRDVFAPIAAELAAGR
                     WQPEALGHAVQELVPGWLDEPVVGPGQVSGAVITIDHFGNLLTNIDAALLPQAPHTVV
                     RAGGHDIPLRRTYSDVRPGDYLALVNSFGVLEVARAEQSAAHGLGLDRGAPVLAMARK
                     GA"
     gene            6946..7620
                     /locus_tag="VD737_08235"
     CDS             6946..7620
                     /locus_tag="VD737_08235"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXW10587.1"
                     /translation="MPYLYLVRHGQPDFAGNYDSLTELGTRQSSWLGEHLAARGIRFA
                     KAASGTLVRQVDTCDAMLQNFPDPPAPVRDARFNEYDHASLLSFFEGDRLQVLRAAGD
                     RRGYFTAIRHSLQQWSRHAGPIANGESWSTFGARILSGVAALCDGLERDANVLIVTSG
                     GVIGRYTAEVLGAGADAAIQLNLQTRNTGVTEIVRTPSGGARLVAFNAVPHLESGDRQ
                     NMVTFS"
     gene            complement(7667..>8256)
                     /locus_tag="VD737_08240"
     CDS             complement(7667..>8256)
                     /locus_tag="VD737_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10588.1"
                     /translation="TFGESSEPVCRPWLAEDPDDEVVTLPQFVRRFPAGTYRVRGLTG
                     DGEPVNGRTALTYWLPAAPDSVAYSGGVITWEAGTSLGACATRDQLWRLVGNGVLPIH
                     PMNVPVAAWEVVLELEDGSHRSFSVRLPARGNRAQTSVTVPAEFLSAVAPDTPAKIEV
                     GAIGGRLATGDDDNATFTELVDLCLNRRNGCPEPE"
BASE COUNT         1290 a         2880 c         2752 g         1334 t
ORIGIN      
        1 cacgccctcg accagcccga cgatcgcagg gcctgcaccg agcgttgcgg tcaggaacag
       61 cggcagcaaa ggcgtgatca tctcgctcgc ggcatcgttc aggaacgaga cgacgccgag
      121 cacgacgacg gtgcgcggca acagcgttcc cgccctcggg cgcgtcgatg tcgcggggtc
      181 accgtccacc gatcatcctc tgtcggacaa cgcacgtttc gctgcgcgcc gcatcgttct
      241 agactgagcg ggttggcgac acgcgagcca ggatagcctc gccgccagcg tcccccacac
      301 catcacaggg aacgctccat gagcatattc ggcaggattc tagagaaact cggtatcggc
      361 aaggacgacg acaagaacaa gggccggacg ccgccggcat cgagcaccca ggcccaggcc
      421 ggccgaccgt cggtggcgcc tacgcagacg acgccgcaca cgtcgtcgcc gggtgcgcaa
      481 agaaccgcgc cgcctccttc accgcagcag gcacaggcgg cgcgtccggc ccaggcgcag
      541 cagccccggg ccatgtccga gaccgacgtg gtggcgaaac tcgagaagct cgccgcggac
      601 aatccgcaga aactcaactg gcgcacgtcg atcgtggacc tgatgaagct gctcggcatg
      661 gagagttcgc tggccgagcg caaggaactg gcggcggagc tcggctatcc gcgcgaccag
      721 atggacgact ccgccaagat gaacgtctgg ctgcacaagc aggtgctgcg caagatcgca
      781 gagaacggcg gcaacgtgcc caaggaactg ctcgactgag cgacactcca cggtgtgcgg
      841 gtgcgtgctg acgcgcgcac ggcgcacgcc tgggcgattt ccgcatgcct gccggccacg
      901 gaacggcccg cgcttgcgcg gctcgggctg cggctcgtgg cggtgggacc gtcagctcaa
      961 gcgaacggtt tggggcgggc ctgcaagagc ctgcgctgca tctcggtttg acgcaggtgc
     1021 gcgagctgca tcctgaggtg gtcggcgatc acgcctcggt cgccgtgccg ggcggcgagt
     1081 cgggcgattg cgtgttcgag cagttcaggc tcggagcaac tcgcgaacgg ccgggttccc
     1141 tggctcgtca tgtgttcccc ctcctcttcc ctggggcacg acggcgcggc gacgccgagt
     1201 gcgcccgcgg gggcaattat acgcccggcc cggacggagg gatccggcgc gcctcgttca
     1261 ggatcggtcg tacttccgcg aactgccgcg gaaccgctcg accggatact tgccggcgtt
     1321 cagttcgagc ttgcgcgccg cagcctcacc gagatcgatg ccggcctggt cggcgagccg
     1381 gatcaggtac agcagcacgt ccgcgagttc cagcgcgacg gcgtcgcgca cgtccggtgg
     1441 caggttgcgg ctctggcttt cggtgagcca ctggaagtgc tcgagcactt cgcccacttc
     1501 accggccagc gccatggcga ggttcttggg cgagtggaac tgctcccagt cacgctcgca
     1561 tgcgaactcc gcgagccgtg ccgcgagcgc ttcgagcgtg ggtgagcggt cggcggccat
     1621 caggccgcgg ccgtctggcg cctggcttcg accaggatgc gcagcatccg ccgtatcggt
     1681 tccgcagcgc cccacagcag ctggtccccc accgtgaacg ccgacacgta ttcaccgccc
     1741 atcgcgagct tgcgcacgcg accgacggga atctgcaacg tgccgctcac cttcgcgggc
     1801 gacagttcgg cgatcgaggc atcgcgcgta ttgggcacga gccggatcca ctcgttgcct
     1861 tgcgccagcg tgcgctcgat ctccgcgagc ggcaggtcgc gtcgcagctt gatcgtcagc
     1921 gcctggctgt ggcagcgcat cgctccgatc cgcacgcaga tgccctcgac cgggatcgtg
     1981 gccgcgccgg ttctaccgag gatcttgttg gtctcggcgc tgcccttcca ttcctcgcgg
     2041 ctctggccgt tgccgaggtc cttgtcgatc cagggaatca ggctgcctgc cagcggcgcg
     2101 ccgaactgcg cctgcggcag ctccgcgctg cgcagtcgcg ccgtcacggc ctggtcgatg
     2161 tcgaggatcg cgcttgccgg gtctcgcagc atggagacgg cacagtcgtg caccgcgccc
     2221 atctgctcga cgagttcgcg catgttcggc gcaccggcgc cggaggccgc ctggtacgtc
     2281 atcgcggtga cccactcgat caggtcggca cggaaaagac cgccgagcgc catcagcatc
     2341 agcgagacgg tgcaattgcc gccgatgtaa tccttgatgc ccgccgcgag cgcccggtcg
     2401 atcacgtcgc ggttgaccgg gtcgaggatg atgacggcgt cgtccttcat gcgcagcgcc
     2461 gaggccgcgt ctatccagta gccctgccag ccggctgccc tcagcttcgg gtgcacggcg
     2521 ttggtgtagt cgccgccctg gcacgtcacg acgacgtcgc actgcgccag cgcttcgatg
     2581 tcgtgggcgt cctgcagcgg tcccgagggc ttgcgcccgc cgacgaccgg cgccgccgcg
     2641 cccgcctgcg acgtgctgaa aaacaacggc tcgacgtggt cgagatcccg ctcggccgcc
     2701 atgcgctgca tcagcaccga gcccaccatt ccgcgccagc ccacgattgc agtcttcaac
     2761 gcgctactcc gatgtcctga aaatggggac actcctattt tccgcggccg agatctgcgg
     2821 gtcccctggg cggcatcccc gcggaaaaaa ggaatgtccc cattttcgct cagacgacca
     2881 cttcgcgcgc cggttcacgc aggttgtatg acgaactcat ggcccggcca taggcacccg
     2941 cgttcgccac cagcaggacg tcgccttcgc gggtcggcgg cagcagtcgt tcgtagccga
     3001 gcacgtcggc cgattcgcag atcggaccga cgacgttgta cacctcggtg cccggttcgc
     3061 catagcgggt caggttcacg atgtcgtggt gtgctccgta gagcgctggc cggatcagtg
     3121 aattcatgcc ggtcgcgacg ccgacgtact gcaccgcccc cttgcccttc agctgggtca
     3181 cctgcacgag cagcacgccg gcctgcgcga ccaggaaacg ccccggctcg atccagagct
     3241 gcagctgtgg atacttgccc cgcagctcgt cgatgacgcc ctgcagcgcc gcgagatcga
     3301 ccggcacctg tccgggcttc tccggcacgc cgagccctcc gcccaggtcg accgcgcgcg
     3361 cgtccggaaa acgcttcgcc agctcgccga gcaggttgcc cacctcctgc cagttgcgca
     3421 cctcgaacac gccgctgccg gtgtgcgcgt gcagcccgac gacgcggcag ccgaccttcc
     3481 tgaccagccg ctccagttcg tcgagctcga acaacggcac gccaaacttc gaatggacgc
     3541 cggccgtgcg cacgtggtcg tgatggccgc gtccgaatcc ggtgtccagg cgcacgagca
     3601 cgtcacgacc gcggaacaga ctgccccact cccgcagcgc gtacaggctg tcgacggtca
     3661 ggttgacgcg cttctccagc gcccactcgt actcggcgcg cggcgcgaag tttggcgtga
     3721 acaggatcct gctgggatcg atgccgcgga cagtgtcgaa cacgtgctgg cactcgccct
     3781 gcgacacgca ttcgaacgac agccccagcc cggcgaacgt ccgcaggatc tcggggtgcg
     3841 gattggcctt gaccgcgtag aagatgcggt ccacgccgcg aatgtccagc aactgccgtg
     3901 ccgcaagttc gagcgatgcg cggtcgtaga cgtaggccgc gccgtgctcg cgggcgatcg
     3961 cctggagttc ctcgcggcgg tcctgccacc agggcggccc tccggccggc agcacagggc
     4021 cgaggccgtg gatctgctcc caggtcgggc cgagcacgtg gtcggacggc gcgttccaga
     4081 cgatgacgtc gtgcagccgc cgcacgagcc ggtcgccctg ctcctcgtcg acgacgaacg
     4141 tgatgttgag gtcgttggcg gcctgcgtga cgaggtagat cttctgctcc tcgaacagct
     4201 cgagcgcgtc accaagccgg tgcagcgtcg cgcggatgct gcggccgacc aggctcacgg
     4261 cggagcacgg cccgatgacc tcgacgcggc acaggcgcga gagatccgtc atcaacgctt
     4321 cgagcgtgcg ctcgtcgagc gcgttcgcgg caggatccaa cgacaccgtc acgctcgtct
     4381 ccgacgtgga gaccaggtcc accgagagcc cgtgctgctt gaacacggcg aacgcgtcgg
     4441 ccaggaaccc gacctggtgc cacatcccga gcgtttccat cgaaagcagc gtgatgcctt
     4501 tcttgatgca gacggccttc actcgcgcgc cgccgtcgcc cccggtcgcc gtgacgaccg
     4561 tgcctgcgag ttccggctgc tgcgtcgcgt gcacgctcag cgggatcgcg tactgcttga
     4621 ccggcaggat gcagcgcggg tgcagcacct tggcgccgct gcttgcgatc tcctgggcct
     4681 cgtcgtagtc cagcgacttg agcaggcgcg cgttggacac cgatcgcgga ttggccgtga
     4741 acatgcccgg cacgtcggtc cagatctcga ggcgtcgcgc acgcagcttc gcggcgaagt
     4801 agctgcccga ggtgtcggag ccgccgcgac cgagtagcac ggtgtcgccc cgggcgtcgc
     4861 tcgcgatgaa cccctgcgtg atcagaaccc tgccgaccga gccgaggcgc tgctgcatgt
     4921 cggcgtccgg ctcgaagttg caggtggccg acaggtaatt ggcgcgctcg gaagcgcccg
     4981 ggcgttcctc ggcgtgcagc agcttgcgcg cgtccagcca ctgggcgtcg acgccctcgt
     5041 gcttcaggaa cgccgcgccg atccgcgtcg ccatgagttc gccgttggcc atgacgcgcg
     5101 cgcgcagacg atcgctcacc tcgccgatca ggtagatgcc ctcggcgctg cggcgcagtt
     5161 cggagaagtg atgatcgagc tcggcggcca cgtccggcac gccgaggtcg cgggccaggt
     5221 ccatgtggcg acgctcgatc tggtccatga ccggttccca ttcgtcggtc agggctttcg
     5281 cgagcagttg ttccagtcgg tcggttatgc ccgagagcgc cgagtgcacg atgacggggt
     5341 ggagaccctc ggcgagccgc tccttgacga cggcggcgac gctcttccag ttgctgacgc
     5401 tcgaaacact ggtgcccccg aatttgagga ccacccagtt gctgtcgcta tggcctgtca
     5461 tcgagcggac cccacggcgc cttgtgatcg cgtggtgcgg cgccttcgtg cttcctgacg
     5521 agtccaggcg cagcgcgcca ggacggtcac cggctccccg agtcaggagc cgggggcccg
     5581 tgcggtgtgg acctactggt cttcgtagta gtccttgagt tctttctgca ggcgcttctc
     5641 ggccaggacg tcctcgagct tgctccaggc cgccttgcct tttttcacgg cctgcttgcg
     5701 gcgatcgact ctgtcgatga agcggtcgta gtcctcgcca tcctcggcgt tggcgacgaa
     5761 gtcttcatca agatcgaatt ggccctcgtc tctttccatt ggtggcccca agaaaagtcc
     5821 tgcaatttca aggttctgcc cgcatctccg caggcacaag gctgcgaact atacacggat
     5881 cggaccgcat ctcaaccggg acttgttgca ctgcaacgtc gcgatggagg ggccagcccg
     5941 gcaggtaaga ccgtgccggc ctgagcgcgg ttccatggac gatcaatgac ttaggcgccc
     6001 ttgcgcgcca tggcaaggac cggggcgccg cggtcgagcc cgagtccgtg ggcggcgctc
     6061 tgctcggccc gcgccacctc gagcactccg aacgagttga cgagggccag gtagtcgccg
     6121 ggccgcacgt cggaatacgt gcgtcgaagc gggatgtcgt ggccgcccgc gcggacgacc
     6181 gtgtgtggcg cctgcggcag caacgctgcg tcgatgttcg tcagcaggtt gccgaagtgg
     6241 tcgatcgtaa tcacggcgcc gctgacctgg ccgggcccga cgaccggctc gtcgagccaa
     6301 cccggcacga gttcctgcac cgcgtggccc agcgcctccg gctgccagcg tcctgccgcc
     6361 agttccgccg cgatcggcgc gaagacgtcg cggccgtgga acgtcgcgct cggccgatgc
     6421 acgtcgaggc ggggcagcac gcccaggtcg aggtgacgca gcgtggccgc cgtttcgacg
     6481 cgttcgagca caccggccag caggccgttg tccggcgcaa gaaacacctg gccatcggcc
     6541 tccacgatcg cgatgtcgcg cgcgctcccg acacctggat ccacgacggc gacgtggacc
     6601 gtgcccggcg gaaagtagcg atacgaccga ccgagccaga accccgcctc tgccggccag
     6661 tgcacgagca cctcgtgggt gaggtcgacc agctgcgccg tcggaaaacg ccgcagcacc
     6721 tgtcccttca tgacaccgac gaacgggtcc ttgtggccga agtcggtcgt gagtgtgatg
     6781 acgcccgacg gcgtgaacgg tgcatggtcg cggtccgggc tgggcatcgt gtgttatccc
     6841 gatagcgtca tcgagggcgg gactcgcacg ataaccgtgc ggacgtccgg ccgggaactg
     6901 ctccgcccgg ctgccgccgg cggcccgaga tagactcccg cgcctatgcc gtatctgtac
     6961 ctcgtccggc acggccagcc cgacttcgcc ggcaactacg attcgctcac cgaactcggc
     7021 acccgacaat cgagctggct cggcgagcac ctggctgcgc gcgggatccg tttcgccaag
     7081 gctgcgtccg gcacgctcgt gcgacaggtg gacacctgcg acgcgatgct gcagaacttt
     7141 ccagatccgc cggcgccggt gcgcgacgcc cggttcaacg agtacgacca cgcgtcgctg
     7201 ctgagcttct tcgagggcga ccggttgcaa gtgttgcgcg cggcgggcga ccggcgcggc
     7261 tatttcacgg ccatccggca ttccctgcag cagtggtcac gtcacgccgg gccgatcgcg
     7321 aacggcgaaa gctggtcgac gttcggcgca cgcatcctgt ccggcgtcgc agccctgtgc
     7381 gacggattgg agcgcgacgc caacgtgctg atcgtgacgt ccggcggcgt gatcggccgc
     7441 tacacggccg aggtgctcgg cgccggcgcc gacgctgcga tccagcttaa cctgcagacg
     7501 cgcaacaccg gcgtgacaga gatcgtgcgc acgccgtcgg gcggtgcgcg tctcgtggcg
     7561 ttcaacgccg tgccgcacct cgaaagcggg gatcgtcaga acatggtcac gttcagctga
     7621 gcaccccggc gccatcggcc gccagcgccg gcggccgact cgaccttcat tccggttctg
     7681 gacacccgtt ccgtcggttg aggcagagat cgacgagctc cgtgaacgtc gcattgtcgt
     7741 cgtcgcccgt cgccagtcga ccgccaatcg ccccgacctc gatcttggcg ggcgtgtccg
     7801 gcgccacggc cgacaggaac tcggccggca cggtcacgct cgtctgcgcc cggttgccgc
     7861 gtgccggcag tcgcaccgag aacgaccggt gcgagccgtc ctcgagctcc agtacgactt
     7921 cccaggctgc gactggcacg ttcatcgggt gtatcggcag cacgccgttg ccgacgagcc
     7981 gccagagttg atcgcgagtg gcgcaggcgc cgagcgacgt gcccgcttcc cacgtgatca
     8041 ccccacccga ataggccacg ctgtccggag ccgcgggcaa ccagtaggtg agcgcggtgc
     8101 ggccgttgac cggttcaccg tcgccggtca gtccccgcac gcggtaggtg ccggccggga
     8161 agcgtcggac gaactgcggg agggtgacga cctcgtcgtc cggatcctcg gcgagccatg
     8221 gccggcacac cggctcggag ctctcgccga acgtct
//
LOCUS       DASPFR010000315         3208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1188854, whole genome shotgun sequence.
ACCESSION   DASPFR010000315 DASPFR010000000
VERSION     DASPFR010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188854"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VD737_08245"
     CDS             <1..297
                     /locus_tag="VD737_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10589.1"
                     /translation="QRAEHEGKRGKITITRFKGLGEMNASQLRESTVHPDTRRLVLLT
                     LEDNGRADAMLDMLLNKKRASDRRDWLETKGNLASVVLPGVEGVATETAAADTE"
     gene            300..2579
                     /gene="parC"
                     /locus_tag="VD737_08250"
     CDS             300..2579
                     /gene="parC"
                     /locus_tag="VD737_08250"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HXW10590.1"
                     /translation="MANTRDTTTTTAALDFEGVERQPLRSFTERAYLEYSMYVILDRA
                     LPHVGDGLKPVQRRIIYAMSELGLAATAKHKKSARTVGDVIGKFHPHGDSACYEAMVL
                     MAQPFSYRYPIVDGQGNFGSPDDPKSFAAMRYTESKLTRYADVLLAELGQGTVDWLPN
                     FDGSLDEPALLPARLPNLLLNGAQGIAVGMATDIPPHNLREVAAACVRLLEEPKTTTR
                     QLCEHVMGPDLPTGAEIITPRDELLKLYETGNGSFRARARFEVEDGEIVVTELPYQVS
                     GSKVLEQIAAQMRAKKLPMIEDLRDESDHEHPTRLVITPRSNRVDTDQVMAHLFATTD
                     LERTYRVNLNVIARDGRPRVMGLKTLLEEWLSFRVDTVTRRLQYRLEKVNARLHILDG
                     LLVAYLNLDEVIRIVRTEDDPKAALIARFNLSEAQAEAILETKLRHLARLEEMKIRGE
                     QAELAKEREQLTKLLGSKAKLRKLVRDEIVADAEKFGDARRSRLVERAAAQAIDETEL
                     VASEPVTVVLSKSGWVRQAKGHEIDPRSLSYKTGDEYRSCARGRSTQFAVFLDSTGRA
                     YSLLAHTLPSARGQGEPLSGRLDPPDGATFPGVMIGEPQDRWVLASSAGYGFVAKLED
                     LYSRNRAGKAVLNVPDGYVAIGPAAIPVVDDPKAVLLAALNSEGRLLTFKVEDLPEMP
                     RGKGNKIFGVSHKKSLVEPETLLAVAAVAPGQTLRLLAGERHMGLTYRELAEYRGERG
                     QRGAVLPRGWRKAEALEVE"
     gene            2751..>3208
                     /locus_tag="VD737_08255"
     CDS             2751..>3208
                     /locus_tag="VD737_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10591.1"
                     /translation="MNKRFVSLGAVAGFAVLGSLVAPAAHAEERVCRGYIGKVTVDNL
                     RVPDNATCTLYGTRVKGTIKVEYGSTLKARRIVVIGNVQAENSSLVQVLENSRVGGSV
                     QLKQGGRASVNDSHVDGDIQFESNNGMLRALRNDVGGNVQIMQNVGGVEVR"
BASE COUNT          579 a         1078 c         1121 g          430 t
ORIGIN      
        1 cagcgcgccg agcacgaggg caagcgcggc aagatcacga tcacccggtt caagggcctg
       61 ggcgagatga acgcgtcgca gctgcgcgag agcaccgtcc atccggacac gcgccgactg
      121 gtgctgctga cgctcgaaga caacggccgc gccgacgcga tgctcgacat gctgctcaac
      181 aagaagcgcg cgtccgaccg ccgtgactgg ctcgagacca agggcaacct cgcgagcgtg
      241 gtgctgcccg gcgtcgaggg cgtcgccacc gagacggccg cggcggacac ggaataggca
      301 tggcgaacac ccgcgacacg accacgacca cggccgcgct cgacttcgaa ggcgtcgagc
      361 gacagccgct gcgcagcttc accgaacgtg cgtacctcga gtactcgatg tacgtgatcc
      421 tcgaccgcgc gctgccacac gtgggcgacg ggctcaagcc cgtgcagcgg cgcatcatct
      481 acgcgatgag cgagctcggc ctcgcggcca ccgccaagca caagaagtcg gcccgcacgg
      541 tcggcgacgt gatcggcaag ttccacccgc acggcgactc ggcctgctac gaggcgatgg
      601 tcctgatggc ccagccgttc tcgtaccgct acccgatcgt ggacgggcag ggcaacttcg
      661 gctcgccgga cgatccgaag tcgttcgcgg cgatgcgcta caccgagtcg aagctcacac
      721 gctatgccga cgtgctgctc gccgaactcg gccagggcac ggtcgactgg ttgccgaact
      781 tcgacggctc gctggacgaa ccggcgctgc tgccggcgcg actgccgaac ctgctgctca
      841 acggcgcgca gggcatcgcc gtcggcatgg cgaccgacat cccgccccac aacctgcgcg
      901 aagtcgcggc cgcgtgcgtg cgcctgctcg aggagccgaa gacgacgacg aggcaactct
      961 gcgagcacgt gatggggccg gacctgccga ccggcgccga gatcatcacg ccgcgcgacg
     1021 agctcctgaa gttgtacgag accggcaacg gctcgttccg ggcgcgggcc cgcttcgagg
     1081 tcgaggacgg cgagatcgtc gtgaccgagc tgccgtacca ggtgtcgggc tcgaaagtgc
     1141 tcgagcagat cgcggcgcag atgcgcgcga agaagctgcc gatgatcgag gacctgcgcg
     1201 acgagtccga ccacgagcac ccgacgcgcc tggtgatcac gccgcgctcc aaccgggtcg
     1261 acaccgacca ggtcatggcg cacctgttcg cgacgacgga cctcgagcgc acgtatcgcg
     1321 tcaacctgaa cgtgatcgcc cgcgacggtc ggccgcgcgt catggggctc aagacgctgc
     1381 tcgaggagtg gctctcgttc cgcgtcgaca cggtcacgcg acgcctgcag taccggctcg
     1441 agaaggtcaa cgcgcggctg cacatcctcg acggcctgct cgtcgcctac ctgaacctgg
     1501 acgaagtgat ccgcatcgtc cgcaccgagg acgacccgaa ggcggccttg atcgcgcgat
     1561 tcaacctctc ggaggcgcag gccgaggcaa tcctcgagac caagctgcgc cacctcgcgc
     1621 ggctcgagga aatgaagatc cgcggtgagc aggcggagct cgccaaggag cgcgagcagc
     1681 tgacgaagct gctcggcagc aaggcgaagc tcaggaagct cgtgcgcgac gagatcgtcg
     1741 ccgacgcgga gaagttcggt gacgcgcgcc gcagcaggct ggtcgagcgc gcggcggccc
     1801 aggcgatcga cgagacggag ctggtcgcga gcgaaccggt gacggtcgtg ctgtcgaaga
     1861 gcggctgggt gcgccaggcc aagggtcacg agatcgaccc gcgctcgttg tcgtacaaga
     1921 ccggcgacga ataccgttcc tgtgctcgcg ggcgcagcac gcagttcgcc gtgttcctgg
     1981 acagcacggg gcgcgcgtac agcctgctcg cgcacacgct gccctcggcg cgcgggcagg
     2041 gcgagccgct ctcggggcgt ctcgacccgc cggacggcgc cacgttcccg ggcgtcatga
     2101 tcggcgagcc gcaggatcgc tgggtgctcg cgagcagcgc cggctatggc ttcgtcgcga
     2161 agctcgagga cctctacagc cgcaatcgcg ccggcaaggc ggtgctcaac gtcccggacg
     2221 gctacgtcgc gatcggcccg gcggcgatac cggtcgtcga cgacccgaaa gcggtcctgc
     2281 tcgcggcgct caactcggaa gggcgcctgc tcacgttcaa ggtagaggac ttgccggaga
     2341 tgccgcgcgg caagggcaac aagatcttcg gcgtgtcgca caagaagtcg ctggtcgagc
     2401 cggagacgct gctcgcggtc gccgcggtgg cgccgggaca gacgttgcgg ctgctggccg
     2461 gcgagcggca catgggcctc acttatcgcg aactcgccga ataccgtggc gagcgagggc
     2521 agcgtggcgc ggtgctgccg cgcggctggc gcaaggccga ggcgctcgag gtcgagtgaa
     2581 acggccccag ggacgacccc cgggttagaa aacggctaga aaactagggc gccccacggc
     2641 gggcgcctac agtgcgcctc agtgaggtgc gcgacgccca ccgttcgacg gcgggcttgg
     2701 cgattccgag ggatccacgc acctccatgg attcacaacg gaggtgcgac atgaacaagc
     2761 gtttcgtttc cctgggggcc gtcgccggct tcgccgtgct cggaagcctg gtggctccgg
     2821 ccgctcacgc cgaggagcgg gtctgccgcg gttacatcgg caaggtcacc gtcgacaacc
     2881 tgcgcgtgcc cgacaatgcg acctgcacgc tgtacgggac acgcgtcaag ggcacgatca
     2941 aggtcgaata cggttcgacg ctcaaggcgc ggcggatcgt ggtgatcggc aacgtccagg
     3001 ccgagaactc cagcctggtc caggtcctcg agaactcccg ggtcggcggc agcgtccagc
     3061 tcaagcaggg tgggcgcgcg tccgtcaacg acagccacgt ggatggcgac atccagttcg
     3121 aaagcaacaa cggcatgctg cgagcgctgc gcaacgacgt cggcggcaac gtccagatca
     3181 tgcagaacgt gggcggcgtg gaggtgcg
//
LOCUS       DASPFR010000316         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_944475, whole genome shotgun sequence.
ACCESSION   DASPFR010000316 DASPFR010000000
VERSION     DASPFR010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944475"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VD737_08260"
     CDS             complement(<1..305)
                     /locus_tag="VD737_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10592.1"
                     /translation="MSDLKARTGGLGPGRFLVTFIVVAIAVTGCSRKQEPVATDDAAA
                     GTAAASADALEGQVDIVAWPGYIERGETDKNYDWVTAFERDTGCKVNVKTAGTSDEMV
                     "
     gene            complement(430..984)
                     /locus_tag="VD737_08265"
     CDS             complement(430..984)
                     /locus_tag="VD737_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HXW10593.1"
                     /translation="MPLRNTAEAYGSLAKFLHWAIVILIVGQWLLAEFAEDAGKGTPE
                     RASLMGWHISFGLLVLMLALVRIGWKVVNRGQPAVLGEVAWQRRAASAGHGLLYLLIL
                     LQPISGWLVVSTGGSGPGFFGLFELPALTAENHDFHETMEEVHETLFNALVVVAVVHV
                     AAALYHHWVLKDATLRRMLPFSRP"
     gene            complement(1013..1738)
                     /locus_tag="VD737_08270"
     CDS             complement(1013..1738)
                     /locus_tag="VD737_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10594.1"
                     /translation="MLGRFLELSVATATVAESLAFYESLGFVQASVGEAWRHPYAVVT
                     DGRMTLGLHGTGDDVSQRLTFVTPDLRSRVDELAELGVVVDHARLDDASLNEVQFRDP
                     AGACVRLLEARTFSPPALEPTFESALGYFEGYVIGTDDLAAAGTFWERFGFVAFQDAG
                     DEELPPRLVASHRDVNLMFLELDLPTPMLCFSAADMPQRVARLQERGFTFARRVPRPL
                     QANGAAVLQAPDGVQVLLVRRAA"
     gene            complement(1752..>2138)
                     /locus_tag="VD737_08275"
     CDS             complement(1752..>2138)
                     /locus_tag="VD737_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HXW10595.1"
                     /translation="GLTEGEWSIGFQSRFGREEWLKPYVDLLLQDYARKGPRQVTVVC
                     PGFATDCLETLEEIAMQNREMFLAQGGEAFDYVPCLNSSPAHVDALEDVVVQHTQGWP
                     ELLGAASDDAARTRARQRALELGAAS"
BASE COUNT          355 a          761 c          709 g          313 t
ORIGIN      
        1 accatctcgt cggacgtgcc ggccgtcttg acgttgacct tgcagccggt gtcccgctcg
       61 aacgcggtca cccagtcgta gttcttgtcg gtctcgccgc gctcgatgta gccgggccag
      121 gcgacgatgt cgacctgtcc ctcgagcgcg tcggcggagg cggccgcggt accagcggcc
      181 gcatcgtcgg tggcgacagg ctcctgcttg cgggagcaac ccgtgaccgc gatcgcgacg
      241 acgatgaacg tcacgaggaa cctgcctggg ccgagaccgc cggtgcgcgc tttcaggtcg
      301 gacactcctg tcgttcgtgt gtgtatggag tcgctgccct gcccgcagca aggcagattc
      361 tatgcccgtt tcggtggccg ggatgcatgg aggcagcgcg ccgagtcccg cccgcgcgcg
      421 gtcgcccgat cagggccgcg agaacggcag catgcgccgc agcgtcgcgt ccttgagcac
      481 ccagtggtgg tacagcgccg cggccacgtg cacgacagcg accacgacca gggcattgaa
      541 gagcgtctcg tgcacttctt ccatggtctc gtggaaatcg tggttttcgg cggtcagggc
      601 cgggagctcg aacagcccga aaaaaccagg acccgagccg ccggtcgaga cgaccagcca
      661 gccgctgatc ggctgcagca ggatcagcag gtacaggagg ccgtgcccgg cggacgccgc
      721 cctgcgctgc caggccacct ctccgagcac cgccggctgc ccccggttga cgactttcca
      781 gccgatccgg acgagggcga gcatcagcac cagcaggccg aacgagatgt gccaacccat
      841 cagcgaggct cgctccggcg tgcccttgcc tgcgtcctcg gcgaactccg ccagcagcca
      901 ctgcccgacg atcaggatca cgatcgccca gtgcaggaac ttcgcgaggc ttccataggc
      961 ttcggcggtg ttgcgcagcg gcatgatgtc tcctggaaag aacgacgaaa ttctacgctg
     1021 cgcgtcgcac cagcagcacc tgcacgccgt cgggcgcctg cagcacggcc gcgccgttcg
     1081 cctgcagcgg ccggggcacg cgtcgtgcga acgtgaagcc gcgctcctgc agccgggcga
     1141 cccgctgcgg catgtcggcc gccgaaaagc agagcatcgg cgtcggcagg tccagctcga
     1201 ggaacatcag gttgacgtcc cggtgcgagg cgaccagccg cggcggcaac tcctcgtcgc
     1261 ccgcgtcctg gaacgcgacg aatccgaacc gctcccagaa cgtaccggcc gccgcgagat
     1321 cgtccgtccc gatcacgtag ccttcgaagt agccgagggc gctctcgaac gtgggctcga
     1381 gcgccggcgg cgaaaacgtg cgcgcctcga gaaggcgcac gcaggcgccc gccgggtcgc
     1441 gaaattgcac ctcgttcagc gacgcgtcgt ccagacgggc gtgatcgacg acgacgccga
     1501 gctcggccag ctcgtcgacc ctcgatcgca ggtcaggcgt gacgaaggtg agccgttgcg
     1561 acacgtcgtc gccggtgccg tgcaggccca gcgtcatccg gccgtcggtg accacggcgt
     1621 acggatgccg ccaggcctca ccgaccgacg cctgcacgaa accaagcgac tcgtagaacg
     1681 cgagcgattc ggcgacggtg gccgtcgcga cgctcagttc cagaaagcgc ccgagcacgg
     1741 cggaccgctg ctcagctcgc cgcgccgagt tcgagcgcgc gttgccgggc gcgcgtgcgc
     1801 gccgcatcgt cggacgccgc gccaaggagt tccggccagc cctgcgtgtg ctgcaccacc
     1861 acgtcttcca gcgcgtcgac gtgggccggg ctcgaattga ggcaaggcac gtagtcgaac
     1921 gcctcgcccc cctgcgccag gaacatctcg cggttctgca tcgcgatttc ctcgagcgtc
     1981 tccaggcagt cggtcgcgaa ccccgggcag accaccgtga cctgccgagg tcccttgcgc
     2041 gcatagtcct gcagcaacag gtcgacgtag ggcttcagcc actcctcgcg cccgaacctg
     2101 gactggaaac cgatcgacca ctcgccttcg gtgaggcc
//
LOCUS       DASPFR010000317        15807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1312840, whole genome shotgun sequence.
ACCESSION   DASPFR010000317 DASPFR010000000
VERSION     DASPFR010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15807
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312840"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1407)
                     /gene="aroA"
                     /locus_tag="VD737_08280"
     CDS             complement(103..1407)
                     /gene="aroA"
                     /locus_tag="VD737_08280"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HXW10596.1"
                     /translation="MGRYRVEPADRVAGSITVPGDKSISHRAVMFGALAQGRTRVRGF
                     LDGEDCLATVRVVEHLGARVERLAATEVIVDGVGLRGLRAPTATLDMGNAGTAMRLFM
                     GLLSAQPFDATLVGDASLMRRPMERVARPLREMGAAIETEDGRPPVRIRGDARLRGVR
                     YEMPVASAQVKSALLLAGLYADGQTTVVEPAVTRDHTERMLQGFGVDVKARDGVVSLS
                     PPGALEGASIQVPGDISSAAFFLVAACLGAREPFMIRDVGVNPTRTGILEMLALMGAD
                     LRMLNHRNWGREPVADIEVRPSRLRGVRVPERLVPLAIDEFPAFFVAAACAEGETLVT
                     GAGELRVKESDRIAAMARGFDVLGIEHEVLPDGLRIRGGRPYGGGTIDSRGDHRVAMA
                     FAVASLRATAPIEILDTANVATSFPGFDVLANSVGLQIAASG"
     gene            complement(1407..2606)
                     /gene="pheA"
                     /locus_tag="VD737_08285"
     CDS             complement(1407..2606)
                     /gene="pheA"
                     /locus_tag="VD737_08285"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017816350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HXW10597.1"
                     /translation="MAARTKKKAAGRSRKRPQQTGKTTSVAGRGPGDEPSALPLDRIR
                     ERIDSVDSQLHALINERARLARQVGVSKHSSGHIVDFYRPEREAQVLRLALARNQEEG
                     GPLRDEEILRLFREIMSACLAQQNALKIGYLGPEGTFTQQAVLKFFGHSVRPLPLTSV
                     EEVFHEVESGAADFGVVPIENSTEGTVNNTLDMFLTSPLKISGEVELRIHQNLMGRMT
                     MLGDVRRVCGHPQSLAQCRGWLQEHMPSVERVIVSSNAEGARRARDEDGTAAIAGAAA
                     AEVYGLTLLVPEIEDRPDNTTRFLVIGRKTANPSDADKTTLLVSAAHTDSPGALYRLL
                     EPLAEHGISMTRIESRPSRRRKWDYVFFIDVDGHAERDPLAPALAKLKSRASLFRIVG
                     SYPKAIL"
     gene            complement(2609..3772)
                     /locus_tag="VD737_08290"
     CDS             complement(2609..3772)
                     /locus_tag="VD737_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HXW10598.1"
                     /translation="MYKILTLNNISVRGLERLPRDRYEVASEIGHPDAVLVRSADMHA
                     MTIPDSVLAVARAGAGTNNVPVAQLSQRGIPVFNAPGANANAVKELVLAGMFLAARNI
                     CQAWAYVRTLEGDDHALETAVEKGKKTFVGHELPGRTLGVVGLGAIGVEVANAALGLG
                     MKVLGYDPQITVQRAWQLSSGVEQALSLDDLFARCDFVTVHVPLNDHTRGLVNAARIK
                     LMRRGSVILNFARAQIVDERAVIDGLDSGHLWAYICDFPTNAIKAHARVVALPHLGAS
                     TGEAEENCAVMVADTLRDFLENGNVRHSVNFPEAVLPRAGECRLAIANANVPNMVGQI
                     STALAEASLNIADLLNKSRGELAYTLIDIDAPVPASVIDRVRSIHGVLSARVL"
     gene            complement(3903..4982)
                     /gene="serC"
                     /locus_tag="VD737_08295"
     CDS             complement(3903..4982)
                     /gene="serC"
                     /locus_tag="VD737_08295"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HXW10599.1"
                     /translation="MSRVYNFSAGPAALPQQVLEQLRDEMLDWRGHGMSVMEMSHRGK
                     AFVGIAQQAEADLRELLEVPSNYKVLFLQGGATAQFAAIPLNLTTPDAVVDIVNTGAW
                     SKKAITEAKKYCKVNVVADAASSNYAHVPPPAEWRRSADAAYFHYTPNETIGGVEFPF
                     IPEVDVPLVADFSSTILSRPLDVSRFGVIYAGAQKNIGPAGLVVVIVRDDLLGRARPG
                     VPTVFDWAAMAGDGSMLNTPATYSWYVAGLVFHWIKQQGGLAAMARLNSAKAAALYGA
                     IDSSGFYRNPVAVECRSWMNVPFTMPTPELDKPFAEGARKAGLVALEGHRSVGGMRAS
                     IYNAMPMAGIEALVEYMAEFQRSHG"
     gene            complement(5119..7764)
                     /gene="gyrA"
                     /locus_tag="VD737_08300"
     CDS             complement(5119..7764)
                     /gene="gyrA"
                     /locus_tag="VD737_08300"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HXW10600.1"
                     /translation="MAEIARELLYVNLEDEMKQSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMRELGNDWNRAYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQPFSMRYLL
                     VDGQGNFGSVDGDAPAAMRYTEVRMSRIAGELLADIDKETVEFVPNYDESETEPAVLP
                     TRVPNLLVNGSSGIAVGMATNIPPHNLNEIVAACIAFIDKPSITIPELMELVPGPDFP
                     TAGIINGAREIFNAYTTGRGRIVVRARAEIEEYDNGREAIIVTELPYQVNKARLLERI
                     ADLVRGKLIDGISELRDESDKDGMRVVVELKRDANAEVVLNNLFKQTPMESVFGVNMV
                     ALLDGQPRLLNLKEMIEAFVRHRREVVTRRTVYDLRKARERGHILEGLAVALANIDEV
                     IALIKASPTPAEARSGLMSREWKGGAVPEMLARAGEVSARPDGLDVSLGLHGGIYRLS
                     EAQAQAILELRLHRLTGLEQDKIIAEFGELLETIRDLSDILARPDRLLQVIRDELAAI
                     REQYGDARRTEILRDESDLSIEDLITPEDVVVTLTHGGYAKATRVSEYRAQGRGGRGV
                     TGATFKDEDFVEKIFVANTHDTMLCFSSRGQLYWLKVYQLPIAARNNRGKPIVNLLPL
                     QDGERITAVLPIREYEEEKFVFMATAMGTVKKTSLVAFSRPRTAGIIAVGLEDGDKLV
                     GVDITDGKRDVMLFTTEGKAIRFAEDEVRAMGRNAAGVRGVRMPDGERVISLIIPDPS
                     GLILVASENGYGKITSVEEFPAHGRGGQGVIAIQTTERNGQLVAATQVTTENELMLMS
                     SSGTIVRTPAGDISVLGRNTQGVRLMKLDEGERLIGVESVDPEPATNGIDGVETPGNG
                     LDADSLASSGQAVVPSDDSAASDPV"
     gene            7954..9288
                     /locus_tag="VD737_08305"
     CDS             7954..9288
                     /locus_tag="VD737_08305"
                     /inference="COORDINATES: protein motif:HMM:NF006549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HXW10601.1"
                     /translation="MESVDTLVCARWVLPVEPDGVVLDDHAVAIRGGRIVALLPIEQA
                     RAQFDATDVIERPHHALLPGLVNAHTHAAMTLLRGRAENVSLGPWLHEAIWPIEQRWI
                     DPEYVRDGTELAIAEMLRGGVTCFGDMHLWPEVVARTAAELHMRASVGLIVVEAATRW
                     ASGPTEYIEKGLALRDEYRGDPLVSTFFAPHAPYTVEDATLLRIRRLADELEIPVAMH
                     LHESAWEVERSRERHGLRPLERLKKLGLANPLLVAVHLTQVEPADVDCIAAAGAAVVH
                     CPQSNLKLGNGVCPVTALLERGVTVALGTDGAASNNDLDVLSEMQTAGLLASGVTGRP
                     GSLVSRDLLRMATLEGARALGLAEATGSLVAGKWADLCCVDLRTPRSWPVHDVEATIV
                     YASGAQQVTDTWVAGRHVYANGALPYLDEVAVLERADAWRTRIDAGHGGSEA"
     gene            9285..10007
                     /gene="ubiG"
                     /locus_tag="VD737_08310"
     CDS             9285..10007
                     /gene="ubiG"
                     /locus_tag="VD737_08310"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HXW10602.1"
                     /translation="MTTAAEKLNVDPAEIARFEAAASRWWDPQGEMKPLHDLNPVRLQ
                     YVERAGPLAGRKILDVGCGGGLLAEAMARKGAVVTGIDLAEDLLQVARLHALEAGVEV
                     DYQLTSAEGHAANHEGAYEIAACMEMLEHVPDPTAVIDALARLVRPGGHVFISTLNRT
                     LKSYAMAVIGAEYVLRLLPAGTHTYEKFIRPSELRQWARAAGLRVIDIAGLDYDPFSR
                     TTQLTADASVNYLMHLRRPEGP"
     gene            10004..10687
                     /gene="gph"
                     /locus_tag="VD737_08315"
     CDS             10004..10687
                     /gene="gph"
                     /locus_tag="VD737_08315"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01449.2"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HXW10603.1"
                     /translation="MTRAAAILFDLDGTLLDTAPDMIGALNRLRVEHDREPLSMSEAR
                     AHVSHGALRLVRLGFPDVHDDALEPLRQRFLQLYAANLADGTCLFPGIDHVLATLEAQ
                     QLPWGVVTNKPGWLTDPLLDRLGLYERACCVVSGDTVAERKPHPLPLLHAAQTTGVAP
                     GQCVYVGDAERDIVAGRAAGMQTVVAAYGYLSQEDQPLLWNPHGVVDRPEELLEWLTL
                     RHHTTAVAP"
     gene            10684..12042
                     /locus_tag="VD737_08320"
     CDS             10684..12042
                     /locus_tag="VD737_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HXW10604.1"
                     /translation="MSEFGTTALLLLLLAAACGLAVGLLLGRRGRHAAEVRLATAEAS
                     LAAVRDVAAERERTLELAMQRLRSGFDALAGQALRGNNELFLQLARQVLGQQQEHSLR
                     NLSEREKAVESMLAPVREALQQTHEQIARIEKERAESFGALRASLENVALGQATLQRE
                     TRNLVTALRRPEVRGRWGEMTLRRLAELAGMVDRCDFVEQAHTPGEDQAVRPDMIVNM
                     PDGRQIVVDVKTPLDAYLTAIEAATEEERAVALRKHAAAVQERVRQLAQKAYWAQFEQ
                     SPDFVVLFIPGDQFLSAAVAEAPTLLEDAIRQHVILATPTSFVALLKAVAYGWRQNAL
                     AENALRIQELGEETYKRLVTFTTHLARVGRTLGQSVEAYNAAVGSLERQVLPGARKFT
                     ELGLRPPRELEEVAPIEKLAREFVNLAASPAGDLAPGDVEGPEMDVPEASATSSPKPS
                     ES"
     gene            12039..12872
                     /locus_tag="VD737_08325"
     CDS             12039..12872
                     /locus_tag="VD737_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HXW10605.1"
                     /translation="MSDDPLKRGTPSGSLRQFAVTYAPPHARPLLDALYAYEAGIADT
                     TRLSSHEVAHTRIGWWRDEVDRLLRGAPRHPVTRALLPLRDRAGEELPLLHEPLVAAE
                     IDLARLTLDDARELERYCFRATGSLQVLAAAASLESGALGDAEREFARRLGTAVRRTE
                     HLRDLRLDLDSGRLRIPLEALTSIGVDPQQLRADDVPPALQRWLDATRGELQQELANV
                     GNLLDRPSRARQRQGLVLAALHRKLLTRIHHGPALARTRAEVPPWIRLWTAWRTALRA
                     A"
     gene            12895..13662
                     /locus_tag="VD737_08330"
     CDS             12895..13662
                     /locus_tag="VD737_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009387635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HXW10606.1"
                     /translation="MTVPTSAELRSYRPTDAALHDRVILVTGASGAVGGAVAMACARH
                     GARVILNGRSVRRLEKLHDDIVATGAARPAIAPLDFEKADAAAYDSLAEAVEQQFGRL
                     DGLAHIAGLLGDRSPIEHYDVPTWMKVMHVNVTAPFILTRTLLPLLQRAPDASIVFTT
                     SGVSVKGRAYWGAYAVSKFADEGLMQVLADELATERPIRANSVNPGAVRSPMRARAYP
                     GEDASTLTAPADVVWPFLYLLGADSRGVTGRRFDAQP"
     gene            complement(13631..14467)
                     /locus_tag="VD737_08335"
     CDS             complement(13631..14467)
                     /locus_tag="VD737_08335"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HXW10607.1"
                     /translation="MSERLHKLLAQHGLGSRRQVESWIREGRVLVNGEPAQVGQPVGP
                     RDRVVVDGRDVTRLLAVERKLQVMLYHKPTGEMLRSRAGDDRAGVELNLPSLHGGRWV
                     AVNALGYGEDGLLILTNEGPFALAIARHNHEVTVEYRVRALRPRQDVDWPEMPTEVEV
                     EGEPVTFSVVEPAGTSGTNMWFRVASERTLPRGAIRALFDAAGLKLNRTMLVQWGPVA
                     LPRDLPRGRSRPLEGEALDALYALAGRNRRSEQQARKRRAPRPPPDRRRKPTAGRRSG
                     GR"
     gene            complement(14448..15251)
                     /gene="scpB"
                     /locus_tag="VD737_08340"
     CDS             complement(14448..15251)
                     /gene="scpB"
                     /locus_tag="VD737_08340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HXW10608.1"
                     /translation="MTEHPLASVVEAVLLAAGRPVSVEQLLELFDEPQRPPAEELHAA
                     LQALQSAYEGRGVELREVASGWRVQVRPQHADVVSRLWQERPSRYSRALLETLALIAY
                     RQPITRGEIEDIRGVTVSTTIMRTLHERSWIRVVGHREVPGRPELLGTTREFLDYFGL
                     KSLDDLPTLAELRDLEDIREQLDLPDQPIGAAAAANDADPPAPDRDDTGPGATSSLEA
                     TAASDEPVASDEDVATDEDVTSDDPEFDGEVHASDEDDASDADLERTPA"
     gene            complement(15248..>15807)
                     /locus_tag="VD737_08345"
     CDS             complement(15248..>15807)
                     /locus_tag="VD737_08345"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HXW10609.1"
                     /translation="MLLPRPNAGDGIEEADPRAELVRRLQEYERFKRAAMDMEELHRV
                     DRDTFPASAELVDRRVVKLQPNVTLQEMLVALKDVMQRAEMFAHHHISREPLSVRQRM
                     SEVLQTMSTRAFVEFVALFRAEEGRMGVTVTFIAVLELLREGLIEIVQSEPYAPIHVR
                     AGAPRPAHAAADTEQAGSGPQGNEP"
BASE COUNT         2364 a         5499 c         5441 g         2503 t
ORIGIN      
        1 gatgggaagc atgaagaagt ggctagtggc cagtggtcag tggctagtgg tcgaacataa
       61 ccacggtaac gaggtacgcc gcttcctact agccactagc cactagccac tagccgctat
      121 ctgcaggccc acgctgttcg cgagcacatc gaacccggga aaggacgtcg ctacgttagc
      181 tgtatccagg atctctatcg gcgcggtcgc gcgcaggctc gccaccgcga aggccatcgc
      241 gacgcggtgg tcgccgcggc tgtcgatggt gccgccgccg taaggacgtc cgccgcggat
      301 gcgcaggccg tcgggcagta cctcgtgctc gatgccgagc acgtcgaagc cccgcgccat
      361 ggccgcgatg cggtcgctct ccttgacgcg caattccccg gcgccggtca ccagcgtctc
      421 gccttcggcg cacgcggccg ccacgaagaa ggccgggaac tcgtcgatcg ccagcggcac
      481 gagtcgctcg gggacgcgta cgccccgcag gcgcgacggt cgcacttcga tgtccgccac
      541 cggctcgcgg ccccagttgc ggtggttgag catccgcagg tccgcgccca tcagcgcgag
      601 catctcgagg atgcccgtgc gggtcgggtt cacgccgacg tcgcgaatca tgaacggctc
      661 gcgcgcgccg aggcacgcgg ccaccaggaa gaacgccgcc gacgaaatgt cacccggcac
      721 ctggatcgag gcgccctcga gcgcaccagg cggcgacagg ctcacgacgc cgtcgcgtgc
      781 cttcacgtcg acgccgaagc cctgcagcat ccgctcggtg tggtcgcggg tgacggccgg
      841 ttccacgacc gtcgtctggc cgtcggcgta cagtccggcg agcagcagcg cggatttcac
      901 ctgggcgctc gccacgggca tctcgtagcg gacgccccgc agccgcgcgt caccgcggat
      961 cctgaccggc ggacgtccgt cctcggtctc gatcgcggcc cccatctcgc gcaacggccg
     1021 ggccacgcgc tccatcggcc ggcgcatcag cgaagcatcg ccgaccagcg tcgcgtcgaa
     1081 cggttgcgcg ctcagcagcc ccatgaacag gcgcatggcc gtgcccgcgt tgcccatgtc
     1141 gagcgtggcc gtgggcgcgc gcaggccgcg caggccgacg ccgtcgacga tcacctcggt
     1201 cgccgccaaa cgctcgacgc gcgccccgag gtgctcgacg acgcgcacgg tcgcgaggca
     1261 gtcctcgccg tcgaggaagc cgcgtacgcg ggtgcgaccc tgcgcgagcg cgccgaacat
     1321 cacggcacgg tgtgaaatgg acttgtcgcc cgggaccgtg atcgagccgg cgactcgatc
     1381 ggccggctcg acgcgatagc gccccatcaa aggatcgcct tgggatacga gccgacgatg
     1441 cggaacaacg acgcgcgcga cttcagcttg gcgagcgccg gcgcgagcgg gtcgcgctcg
     1501 gcgtgcccgt cgacgtcgat gaagaacacg tagtcccact tcctgcggcg cgacggccgg
     1561 gactcgatgc gcgtcatgct gatgccgtgc tcggccagcg gctcgagcag ccgatagagc
     1621 gcgcccggcg aatccgtgtg cgcggcggac accagcagcg tcgtcttgtc ggcgtcgctc
     1681 gggttggcgg tcttgcggcc gatcaccagg aaccgcgtgg tgttgtccgg ccggtcttcg
     1741 atctccggca cgagcagggt caggccgtag acctccgccg ccgcagcccc ggcgatcgcg
     1801 gcggtgccgt cctcgtcgcg cgcgcgacgc gcgccttcgg cattgctgct cacgatcacc
     1861 cgctcgaccg acggcatgtg ctcctgcagc cagccacggc actgcgcgag cgactgagga
     1921 tggccgcaga cgcgccggac gtcgccgagc atcgtcatgc gccccatcag gttctggtgt
     1981 atccgcagtt ccacctcgcc cgaaatcttg agcggagacg tcaggaacat gtcgagcgtg
     2041 ttgttgacgg tgccctcggt cgagttctcg atcggcacca cgccgaagtc ggccgcgccg
     2101 gactcgactt cgtggaacac ttcctcgaca gaggtcaacg gcagcgggcg caccgaatgc
     2161 ccgaagaact tgagcaccgc ctgctgcgtg aacgtgccct cgggacccag gtacccgatc
     2221 ttgagcgcat tctgctgcgc caggcacgcg gacatgatct cgcggaacag ccgcaggatt
     2281 tcctcgtcac gcagcggccc gccctcttcc tggttgcggg cgagcgcaag gcgcagcacc
     2341 tgcgcctccc gctcggggcg gtagaagtcg acgatgtgcc ccgacgaatg cttggagacg
     2401 cccacctggc gggcgagccg cgcgcgttcg ttgatcaggg cgtgcaactg cgaatcgacc
     2461 gagtcgatcc gctcgcggat ccggtcgagc ggcaacgccg atggctcgtc ccccggcccg
     2521 cgccctgcaa ccgaggttgt cttcccggtc tgctggggcc gcttcctcga tcgaccggcg
     2581 gccttcttct tcgtcctggc ggccatgctc acaacacgcg cgcgctcaac acgccgtgga
     2641 tcgaccgcac gcggtcgatc accgacgcgg gcacgggcgc gtcgatgtcg atcagtgtgt
     2701 aggcgagttc accgcgcgac ttgttgagca ggtccgcgat gttgaggctc gcctcggcga
     2761 gcgcggtcga gatctggccc accatgttcg gcacgttcgc attggcgatc gcgaggcggc
     2821 actcgccggc ccgcggcagc accgcctctg gaaagttcac ggagtgccgc acgttgccgt
     2881 tctcgaggaa gtcgcgaagc gtgtccgcga ccatcaccgc gcagttctcc tcggcctcac
     2941 cggtcgaagc gccgaggtgc ggcaacgcaa cgacgcgggc atgcgccttg atcgcgttgg
     3001 tcgggaagtc gcagatgtag gcccagaggt ggccgctgtc gaggccgtcg atcacggcgc
     3061 gctcgtccac gatctgggcg cgagcaaagt tgagtatcac gctgccgcga cgcatcagct
     3121 tgatgcgcgc cgcgttgacg agcccgcgcg tgtggtcgtt gagcggcacg tgcaccgtca
     3181 cgaagtcgca gcgtgcgaac aggtcgtcga ggctcagggc ctgttcgacg ccggaggaca
     3241 gctgccacgc gcgctgcacg gtgatctgcg ggtcgtaacc gagcactttc atcccgagcc
     3301 cgagcgcggc gttggccact tccacgccga tcgcgccgag accgacgacg ccgagcgtgc
     3361 ggcccggcag ttcgtgcccg acgaacgtct tcttgccctt ctcgaccgcc gtctccaggg
     3421 cgtggtcgtc gccctcgagc gtgcgcacgt acgcccatgc ctggcagatg ttgcgcgcgg
     3481 ccaggaacat gccggccagc acgagttcct tcaccgcgtt ggcattcgca ccgggcgcgt
     3541 tgaagaccgg gatgccccgc tggctgagtt gcgcgaccgg cacgttgttg gtgccggccc
     3601 cggctcgcgc gacggcgagg acgctgtccg gaatcgtcat cgcgtgcatg tccgccgagc
     3661 gcacgagcac ggcgtccggg tgaccgatct cggaagccac ctcgtagcga tcgcgcggca
     3721 atcgctcgag gccgcggacg gaaatgttgt tgagagtgag gattttgtac atcagtgact
     3781 agtggctagt ggctagtggc tagtggtcag tggtgaatgg ccggttgctg tcgccgacaa
     3841 ggaagcccgt cgtctggtac acgctccgac gagccactga ccactgacca ctagccactt
     3901 tcctatccgt ggctccgctg gaattcagcc atgtactcca ccagcgcctc gatgccggcc
     3961 atcggcatgg cgttgtagat gctcgcgcgc atgccgccga ccgagcggtg gccctcgagc
     4021 gcgacgagcc cggccttgcg cgccccttcg gcgaacggct tgtcgagttc gggcgtgggc
     4081 atcgtgaacg gcacgttcat ccacgaccgg cactcgaccg ccacggggtt tcgatagaac
     4141 cccgagctgt cgatggcgcc gtagagcgcg gcggccttgg ccgagttgag cctcgccatc
     4201 gcagcgagcc cgccctgttg cttgatccag tggaacacga gcccggctac gtaccaggag
     4261 taggtagccg gcgtattgag catcgaaccg tcgcccgcca tcgctgccca gtcgaacacg
     4321 gtcggcacgc ccgggcgcgc gcgtccgagc aggtcatcgc ggacgatcac gaccacgagt
     4381 cccgcggggc cgatgttctt ctgcgcgccc gcatagatca cgccgaaccg gctgacgtcg
     4441 agcggtcgcg acaggatcgt cgacgagaaa tccgcgacca gcggcacgtc gacctccgga
     4501 atgaacggaa actcgacgcc gccgatcgtc tcgttcggcg tgtagtggaa ataggcggca
     4561 tccgcagacc gacgccactc ggccggcggc ggcacgtgcg catagttcga cgacgccgca
     4621 tcggcgacca cgttgacctt gcagtatttc ttcgcttcgg tgatcgcctt cttggaccag
     4681 gcccccgtat tgacgatgtc gacgacggcg tccggcgtcg tcaggttgag cgggatcgcc
     4741 gcgaactgtg ccgtggcgcc accctgcagg aacagcacct tgtagttcga cggcacctcc
     4801 agcagctcgc gcagatcggc ctcggcctgc tgcgcgatgc cgacgaaggc cttgccgcgg
     4861 tggctcatct ccatgacgga catgccgtgg ccacgccagt cgagcatctc gtcacgcagt
     4921 tgctcgagca cttgctgggg cagggcggcc gggccggcac tgaagttgta cacgcggctc
     4981 atggacgacg gagtctccgg gtcgttttgc actgcagtct agcagcagtg tggtccatgg
     5041 agacgacatc gcgacggcat gtgctcatgc cgccgcggtc gtccggaatc cggagcgcga
     5101 gaccggttct cgggtcgact acaccgggtc gctcgcggcc gagtcgtcgc tgggaaccac
     5161 cgcctgaccc gagctcgcga gcgagtccgc atcgagcccg ttgccgggcg tctcgacgcc
     5221 gtcgatcccg ttggtagcgg gttcggggtc gaccgactcg acgccgatca ggcgctcgcc
     5281 ttcgtcgagt ttcatcagcc gcacgccttg cgtgttgcgg ccgagcacgg agatgtcgcc
     5341 tgccggagtg cggacgatcg taccgctcga gctcatcagc atcagctcgt tctcggtcgt
     5401 gacctgcgtg gccgcgacga gctggccgtt gcgttcggtg gtctggatgg caatgacccc
     5461 ctgaccgccg cgaccatgcg cggggaactc ctcgaccgac gtgatcttgc cgtagccatt
     5521 ctcggaggcc accaggatca ggccgctcgg gtcggggatg atcagcgaga tcacccgctc
     5581 gccgtccggc atgcgcacgc cgcgcacgcc ggcggcgttg cggcccatcg cgcggacctc
     5641 gtcctcggcg aagcggatcg ccttgccttc tgtcgtgaac agcatgacgt cgcgcttgcc
     5701 gtcggtgatg tccacgccga cgagcttgtc gccgtcctcg aggccgaccg cgatgattcc
     5761 ggccgtgcgc gggcgcgaga acgccacgag cgaggtcttc ttgacggtgc ccatggccgt
     5821 ggccatgaac acgaacttct cctcttcgta ctcgcggatc ggcagcacgg cggtgatgcg
     5881 ctcgccgtcc tgcagcggca gcaggttcac gatcggcttg ccgcggttgt tgcgggccgc
     5941 gatcggcagc tggtagacct tgagccagta gagctggcca cggctcgaga agcacagcat
     6001 cgtgtcgtgc gtgttggcca cgaagatctt ctcgacgaaa tcctcgtcct tgaacgtggc
     6061 ccccgtcacg cccctgccgc cgcgaccctg ggcgcggtac tcgctgacgc gcgtcgcctt
     6121 cgcatagccg ccgtgcgtca gcgtgacgac gacgtcctcg ggcgtgatca ggtcctcgat
     6181 cgagaggtcg gactcgtcgc gcaggatctc cgtgcggcgc gcgtcgccgt actgttcgcg
     6241 gatggcagcc agctcgtcgc ggatcacctg cagcagacgg tccggacgcg cgaggatgtc
     6301 gctcaggtcg cggatggtct cgaggagctc cccgaactcc gcgatgatct tgtcctgttc
     6361 gaggccggtg agccggtgca ggcgcagctc gaggatcgcc tgtgcctgcg cttcggacag
     6421 ccggtagatc ccgccgtgca gcccgagcga gacgtcgagc ccgtccggac gcgcgctcac
     6481 ctcgccggcg cgcgcgagca tctcgggcac ggccccgccc ttccactcgc gcgacatcag
     6541 gccggacctg gcctcggcgg gtgtcggcga cgccttgatc agcgcgatca cctcgtcgat
     6601 gttggccagc gcgacggcca ggccttcgag gatgtgaccg cgctcgcgcg ccttgcgcag
     6661 gtcgtagacc gtccggcgcg tcacgacttc gcgtcggtgg cggacgaacg cctcgatcat
     6721 ctccttgagg ttgaggagcc gcggctgccc gtcgagcagc gccaccatgt tgacgccgaa
     6781 gacggactcc atcggcgtct gcttgaacag gttgttgagg acgacctcgg cgttggcgtc
     6841 gcgcttgagc tccacgacga cgcgcatgcc gtccttgtcg ctctcgtcgc gcagttcgga
     6901 gatgccgtcg atcagtttgc cgcgaacgag gtccgcgatg cgctcgagca gccgcgcctt
     6961 gttcacctgg tacggcagct cggtgacgat gatcgcctcg cggccgttgt cgtattcctc
     7021 gatctcggcc cgggcacgca cgacgatccg gccgcggccg gtcgtgtacg cgttgaagat
     7081 ctcgcgggcg ccgttgatga tgccggccgt cgggaagtcc ggccccggca ccagttccat
     7141 gagctccggg atcgtgatcg acggcttgtc gatgaacgcg atgcaggcgg cgacgatctc
     7201 gttgaggttg tggggcggga tgttggtcgc catcccgacc gcgatgcccg acgagccgtt
     7261 gaccagcagg ttgggcaccc gcgtcgggag caccgccggc tcggtctcgg attcgtcgta
     7321 gttggggacg aactcgacgg tttccttgtc gatgtccgcg agcagctcgc cggcgatccg
     7381 cgacatgcgc acttcggtgt agcgcatcgc cgcgggcgcg tcgccgtcca ccgagccgaa
     7441 gttgccctgc ccgtcgacca gcaggtagcg catcgagaac ggctgcgcca tgcgcacgat
     7501 cgtgtcgtag accgccgtgt cgccgtgcgg gtggtacttg ccgatcacgt cgccgacgac
     7561 gcgcgcggac ttcttgtagg cacggttcca gtcgttgccg agctcgcgca tcgcgtagag
     7621 cacgcgtcgg tgcaccggct tgagcccgtc ccggacgtcg ggcagcgcac gccccacgat
     7681 cacgctcatc gcgtaatcga ggtacgactg cttcatctcg tcttcgaggt tgacgtacag
     7741 gagttcgcgc gcgatttcag ccatgaatcc gtctgttccg ggcgacccgg ggaggtcgcc
     7801 gcaaagtggt ttttcgagtg gtccgggagc ggtccaaaaa gacggtaaat gttagcacga
     7861 gacggcgtcc aaatgcccgt cgcggaacgg ctttttccgg tgacggtcgc gctcgcccgc
     7921 tatacttccc gcccccgatc cggaagtccc cgaatggagt ccgtcgacac gctcgtctgc
     7981 gcgcgctggg tgctgcccgt cgaacccgac ggagtcgtgc tcgacgatca cgccgtcgcg
     8041 atacgcggtg ggcggatcgt cgcgctgctg ccgatcgagc aggcccgcgc gcaattcgac
     8101 gccaccgacg tcatcgagcg tccgcaccac gctctgctgc cggggttggt caacgcgcac
     8161 acgcacgcgg cgatgacctt gctgcgtgga cgtgccgaga acgtatccct gggcccctgg
     8221 ctgcacgagg cgatctggcc gatcgagcag cgctggatcg atcccgagta cgtacgcgac
     8281 ggcaccgaac tcgcgatcgc cgagatgctc cgcggtggcg tgacctgctt cggcgacatg
     8341 cacctgtggc ccgaagtcgt ggcgcgcacc gcggccgagc tgcacatgcg cgcgagcgtc
     8401 ggactgatag tcgtggaggc cgcgacgcgc tgggcctcgg gccccaccga gtacatcgag
     8461 aaagggctgg ccttgcgcga cgagtaccgc ggcgatccgc tggtttcgac gttcttcgcg
     8521 cctcacgccc cgtacaccgt cgaggacgcc acgctgctgc gcatccggcg gctcgccgac
     8581 gagctcgaga ttccggtcgc gatgcacctg cacgaatcgg cttgggaagt cgaacgctca
     8641 cgcgagcggc acggcctgcg gccgctcgag cggctgaaga aactcgggct cgccaatccg
     8701 ctgctcgtcg ccgtgcacct gacgcaggtc gagcccgccg acgtcgactg catcgccgcc
     8761 gcgggcgccg ccgtcgtgca ctgcccgcag tcgaacctga agctcggcaa cggcgtgtgc
     8821 cccgtgacgg cgctgctcga gcgcggcgtg acggtcgcgc tcggcaccga cggtgccgcc
     8881 tcgaacaacg acctcgacgt gctgtccgag atgcagacgg cgggcctgct cgcctcgggc
     8941 gtcacgggcc gcccgggcag cctcgtgtcc agggacctgc tgcgcatggc gacgctcgag
     9001 ggcgcgcgag cgctcgggct cgcggaggcg accggcagcc tggtggccgg caagtgggcg
     9061 gatctgtgct gcgtcgacct gcgtacgccg cgcagctggc cggtgcacga cgtcgaagcg
     9121 accatcgtgt atgcgagcgg tgcccagcag gtgaccgaca cctgggtcgc gggacgccat
     9181 gtgtacgcga acggggcgtt gccgtatctg gacgaagtgg ccgtgctgga acgggccgac
     9241 gcgtggcgca cgcgcatcga cgccgggcac ggcgggagcg aggcatgacg acggccgctg
     9301 agaaactgaa cgtcgatccg gcggagatcg cgcggttcga ggcagcggcg agccgctggt
     9361 gggatccgca gggcgagatg aagccgctgc acgacctcaa tcccgtgcgg ctgcagtacg
     9421 tcgaacgcgc cggaccgctg gccggccgca agatcctcga cgtcggctgc ggcggtggcc
     9481 tgctggccga ggcgatggcg cgcaagggcg cagtcgtgac gggcatcgac ctcgccgagg
     9541 acctgctcca ggtggcgcgc ctgcacgcct tagaggccgg cgtggaggtg gattaccagt
     9601 tgacctccgc cgagggccac gcggccaatc acgaaggcgc ctacgaaatc gcggcctgca
     9661 tggagatgct cgagcacgta ccggatccga cggccgtgat cgacgcgctc gcgaggctgg
     9721 tccgtcccgg tggccacgtg ttcatctcga ccctcaaccg cacgctcaag tcgtacgcga
     9781 tggccgtgat cggcgcggag tatgtcctgc ggctgctgcc ggccggcacg cacacctacg
     9841 agaagttcat ccggccgtcg gaactgcgcc agtgggcgcg ggcggccggg ctgcgcgtga
     9901 tcgacatcgc cgggctcgac tacgatccat tctcgcgcac gacgcagctg accgcggacg
     9961 cgagcgtcaa ctacctgatg cacctgcgcc gccccgaggg cccatgaccc gggcggctgc
    10021 gatcctcttc gacctcgacg gcacgctgct cgacacggcc cccgacatga tcggcgcgct
    10081 gaaccggctg cgggtcgagc acgaccgcga gccgctgtcg atgtccgagg cgcgcgcgca
    10141 cgtctcgcac ggcgccttgc gcctggtccg gctcggcttt ccggacgtgc acgacgacgc
    10201 gctcgagcca ctgcggcagc gatttctgca actgtacgcg gcgaacctgg ccgacggcac
    10261 gtgcctgttc cccggcatcg atcacgtcct tgcgacgctg gaagcacagc agctgccctg
    10321 gggcgtcgtc accaacaaac ccggctggct cacggacccg ctgctcgacc ggctgggcct
    10381 ctacgagcgt gcctgctgcg tcgtcagcgg cgacacggtg gcggagcgca agccgcaccc
    10441 gttgccgctg ttgcacgcgg cacagacgac cggcgtggca ccgggccagt gcgtctacgt
    10501 cggcgacgcc gagcgcgaca tcgttgccgg acgcgcggcg gggatgcaga cggtcgtcgc
    10561 ggcctacggg tacctttccc aggaggacca gccactgctc tggaacccgc acggcgtcgt
    10621 cgaccgaccc gaagagctgc tcgagtggct gacgctgcgc catcacacga cggcggtggc
    10681 gccttgagcg aattcggcac caccgcgctg ctcctgttgc tgctggcggc ggcatgcggt
    10741 ctcgccgtcg gcttgctgct cggtcgtcgc ggtcgtcacg ccgccgaggt gcgattggcg
    10801 accgccgagg cttcactcgc tgccgtcagg gacgtcgccg cggagcgcga gcgcacgctc
    10861 gaactcgcca tgcagcgctt gcgcagcggc ttcgacgccc tggccggcca ggcgctccgt
    10921 ggcaacaacg agttgttcct gcagctcgca cggcaggtgc tcggccagca gcaggaacat
    10981 tcgctgcgca acctttcgga gcgcgagaag gccgtcgagt cgatgctcgc ccccgtgcgc
    11041 gaggcgctgc agcagacgca cgagcagatc gcccgcatcg agaaggaacg ggcggaaagc
    11101 ttcggggcac tgcgggcctc gctcgagaac gtcgcgctgg gacaggcgac gctgcagcgc
    11161 gagacgcgca acctcgtcac ggccctgcgc cgtccggaag tccgcggccg ctggggtgag
    11221 atgacgctgc gccggctcgc ggagctggca ggcatggtcg atcgctgcga tttcgtcgag
    11281 caggcacaca cgcccggcga ggaccaggcg gtgcggcccg acatgatcgt caacatgccc
    11341 gacgggcggc agatcgtcgt cgacgtgaag acgccgctcg acgcctacct caccgcgatc
    11401 gaagccgcga cggaggaaga gcgcgcggtg gcgctgcgca agcacgcggc ggccgtgcag
    11461 gaacgcgtgc ggcagctggc gcagaaggcc tactgggccc agttcgagca gagccccgac
    11521 ttcgtcgtgc tgttcatccc gggggaccag ttcctgagtg ccgcggtggc ggaggcgccg
    11581 acgctgctcg aggacgcgat ccgccagcac gtcatcctcg cgacgccgac cagcttcgtg
    11641 gcgctgttga aggccgttgc gtacggctgg cgccagaacg cgctcgccga gaacgccctg
    11701 cgcatccagg agctcggcga ggagacctac aagcggctgg tgacgttcac gacgcacctg
    11761 gcacgcgtcg gacggacgct gggccagagc gtggaagcgt acaacgccgc ggtcggctcg
    11821 ctcgaacgcc aggtgctgcc gggcgcgcgc aagttcaccg agctgggcct gcgtccgcct
    11881 cgtgaactcg aggaagtcgc gccgatcgag aaactggccc gtgaattcgt caacctggcc
    11941 gcgtctcccg cgggcgacct cgctcccgga gacgtcgaag ggccggaaat ggacgtgccg
    12001 gaagcgtccg ccacgtcgtc gcccaagccg agcgagtcgt gagcgacgat ccgctcaagc
    12061 gcggcacgcc ctcgggatcg ctgcgccagt tcgccgtcac ctatgcgccg ccgcacgcgc
    12121 gcccgctact ggatgcgctg tacgcatacg aggccgggat cgccgacacg acgcggctgt
    12181 cgagccacga ggtcgcgcac acccgcatcg gatggtggcg cgacgaggtg gaccgtctgc
    12241 tgcggggcgc gccccggcat cccgtcacgc gagccttgtt gccgttgcgc gaccgcgcgg
    12301 gcgaggagct gccgctgctg cacgagccgc tcgtcgcggc ggagatcgac ctggcgcgcc
    12361 tgacgctcga cgacgcgcgc gagctcgaac gttattgttt tcgcgcgacc ggctcgctgc
    12421 aggtcctggc cgcggcggcg agcctggagt cgggcgcgct cggcgatgcg gaacgcgaat
    12481 ttgcgcggcg cctgggcacg gccgttcggc gcaccgaaca cctgcgggac ctgcgcctcg
    12541 acctggacag cggccggctg cgcattccgc tcgaggcgct gacgtcgatc ggcgtcgatc
    12601 cgcaacaact gcgcgcggac gacgtgccac cggcgctgca gcggtggctg gacgcgacgc
    12661 ggggcgaact gcagcaggaa cttgccaacg tgggcaacct tctcgaccgg ccgtcgcgtg
    12721 ctcgccagcg gcagggactc gtgctcgcgg cgttgcaccg caagctgctc acacggatcc
    12781 atcacggccc tgctctcgca cgcacccgcg cggaagtacc accgtggatc cggctgtgga
    12841 cggcctggcg cacggccctg cgagccgcct gaagacattc gcgttggagc ttcgatgacc
    12901 gtgccgacgt ccgccgaact tcgttcctat cgtccgaccg acgcggcgct gcacgaccgc
    12961 gtgatcctgg tcaccggcgc cagtggtgcc gtgggcgggg ctgtcgcgat ggcctgcgcc
    13021 cggcacggcg cgcgcgtcat cctgaacggt cgctccgtgc gcaggctcga aaagctgcac
    13081 gacgacatcg ttgcgaccgg ggcagcacgc cccgcgatcg cgccgctcga cttcgaaaag
    13141 gcggacgcgg cggcgtatga ctccctggcc gaagcggtcg agcagcagtt cggccggctc
    13201 gacgggctcg cgcacatcgc cggcctgctg ggcgatcgct cgccgatcga gcactacgac
    13261 gtgcccacgt ggatgaaggt gatgcacgtc aacgtaaccg cgccgttcat tctcacgcgg
    13321 acgctgctgc ccctcctgca acgggcgccc gacgcctcga tcgtattcac gaccagcggc
    13381 gtgtcggtga agggacgcgc gtactggggc gcctacgcag tctcgaagtt cgcggacgaa
    13441 ggcctcatgc aggtgctcgc cgacgagctc gcgactgaac gaccgatccg cgcgaacagc
    13501 gtgaatccgg gggcggtgcg ctcgccgatg cgcgccaggg cctatccggg cgaggacgcg
    13561 tcgacgctca ctgcgcccgc cgacgtcgtc tggcccttcc tgtacctgct cggggccgac
    13621 agccgcggcg tcaccggccg ccgcttcgac gcccagccgt aggtttgcga cgccggtccg
    13681 gtggtggtcg cggagcccgt cgcttgcgtg cctgctgctc gctgcgacgg ttacggcccg
    13741 caagcgcgta cagcgcatcg agcgcctcgc cttcgagcgg ccgggagcga ccgcgcggca
    13801 ggtcccgcgg caatgccacg ggcccccact gcacgagcat ggtgcgattg agcttgaggc
    13861 cggcagcgtc gaacagcgcg cggatcgccc cgcgcggcaa ggtgcgctcg ctggccaccc
    13921 ggaaccacat gttggtgccg ctcgtgccgg cgggctcgac gacactgaac gtgaccggct
    13981 cgccctccac ctcgacctcg gtgggcatct ctggccagtc cacgtcctgg cggggccgca
    14041 atgcccgcac gcggtactcg actgtgacct cgtggttgtg gcgcgcgatt gccagcgcga
    14101 acggaccctc gttggtgagg atcagcaggc cgtcttcgcc gtagcccagt gcgttgacgg
    14161 ccacccagcg gccgccgtgc agcgacggca ggttcagctc gacgcccgcc cggtcgtcgc
    14221 ccgcgcgact gcgcagcatc tccccggtgg gcttgtgata gagcatcacc tgcagcttgc
    14281 gttccaccgc cagcagccga gtgacgtccc gcccgtcgac gacgacccga tcgcggggac
    14341 cgacgggctg tccgacctgc gccggctcgc cgttgacgag gacgcgacct tcgcggatcc
    14401 aggactcgac ctggcggcgc gaaccgagac cgtgctgcgc gagcagctta tgcaggcgtt
    14461 cgctcaaggt ccgcgtcgct cgcgtcgtcc tcgtcgctcg catgtacctc gccgtcgaac
    14521 tccggatcgt cgctggtcac gtcttcgtcg gtggcgacgt cttcgtcgct cgcaacaggc
    14581 tcgtcgctgg cggccgtggc ctcgagactc gacgtcgccc cgggaccggt gtcgtcccgg
    14641 tccggagccg gtggatccgc atcattggcg gcggccgccg cgccgatcgg ctggtcgggc
    14701 aggtccagct gctcgcgaat gtcctcgagg tcgcgcagct cggcgagcgt cggcaggtcg
    14761 tccaggctct tgagcccgaa gtagtcgagg aactcgcgcg tcgtaccgag cagctcgggc
    14821 cgccccggca cttcccggtg gccgacgacg cgaatccagc tgcgctcgtg gagcgtgcgc
    14881 atgatcgtcg tcgagacggt gacgccacgg atgtcctcga tttcgccgcg cgtaatcggt
    14941 tgccgatagg cgatcagcgc cagtgtttcc agcagcgcac gtgaatagcg gctcggccgt
    15001 tcctgccaca gccgcgagac gacgtcggcg tgctgtggac gcacctgcac gcgccagccg
    15061 ctcgcgactt cgcgcagctc gacgccgcgg ccttcgtacg cggactgcag cgcctgcagg
    15121 gccgcgtgca gctcctcggc gggcggacgt tgaggttcgt cgaacagctc gagcagctgc
    15181 tcgaccgaga cggggcggcc ggcggcgagc agcacggcct cgacgacgct ggccagcggg
    15241 tgttcggtca tggttcgttg ccctgcgggc cactgcccgc ctgctcggtg tcggccgcgg
    15301 cgtgcgccgg gcgcggtgcg ccggcccgca cgtggatcgg cgcgtacggt tcggactgca
    15361 cgatctcgat cagcccctcg cgcagcagct cgagcaccgc gatgaacgtg acggtcacgc
    15421 ccatgcgtcc ctcctcggcg cgaaagagcg cgacgaattc gacgaacgcg cgcgtgctca
    15481 tggtctgcag cacttcggac atccgctgcc gcaccgacag cggctcacgg ctgatgtggt
    15541 ggtgcgcgaa catttcagcg cgctgcatga cgtccttgag cgcgaccagc atctcctgca
    15601 gcgtgacgtt gggctgcagc ttgacgacgc ggcggtcgac cagctcggcc gaggccggga
    15661 acgtgtcgcg gtcgacgcgg tgcagctcct ccatgtccat ggctgcacgc ttgaaacgct
    15721 cgtactcctg cagccgccgc acgagctcgg cgcgcggatc ggcctcctcg atgccgtcgc
    15781 ccgcgttcgg gcgcggcagc agcatgc
//
LOCUS       DASPFR010000318         3715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_580706, whole genome shotgun sequence.
ACCESSION   DASPFR010000318 DASPFR010000000
VERSION     DASPFR010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580706"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..1796
                     /locus_tag="VD737_08350"
     CDS             18..1796
                     /locus_tag="VD737_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXW10610.1"
                     /translation="MPTAVARSRKSTNRPAPPSTPAAPKARKSRAKNPAGIALLPPED
                     LARMRIQVTTIGDLLLTAADRHPESLALVFPDRTITYRELADNALRRARALQALGVRP
                     REHVGVLMHTCAEFVETFFAISFCGAVIVPINARYKSSELAYVIENGDLVTVVTTDAV
                     ADSVNFVERLNGALKDLPRSRNARELKLAGTPKLRNVVLLGKTRAGGYLGEAEFEQAA
                     REVEESEVHRARLRVRVRDVGLMLYTSGTTANPKGCLITHEAMVRNSIALGRHRYRLT
                     HDDRMWSPLPMFHIAAVLPMLAIFDVGGSYCTMGYFDAGVALQMLEKYRVTAMYPSFV
                     TIMQGLVYHPSFAGVDLSRVKLMNSNFAVQPPGVAEPIMTAMPQALQVGSFGMTEAAG
                     TVCTGGWDEAEARRITRLGRPLPGLEVRVINPETGRDLKNGFRGEVLVRGYSLFEGYY
                     KDAEKTAQALDADGWFHTGDIGSLDDDGTIMFHGRIKDMLKVGGENVAAAEIEGLLQR
                     HPAVKLAQVVGIPDAKYVEVPAAFVELKPGTKAGEKELIEFCRKEISSFKVPRHVRFV
                     TEWPMSSSKIQKFRLRQALVAELGLT"
     gene            1798..2334
                     /locus_tag="VD737_08355"
     CDS             1798..2334
                     /locus_tag="VD737_08355"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXW10611.1"
                     /translation="MASANPRTPFGPVMQLGFVVPDLERAMEHWAGTVGVGPFFVLSH
                     VQFAETYYKGQPTRFDMSVGVAQWGAVQVELIQQFDDVPSIYSEFPGRALGGLQHLGV
                     MTRSVADDLERLRGRGIEPVQWGSTANGIRFAYVSTDQHPGAMVELIEHGPAIDGFFG
                     MVREAARDWDGRQPVRRL"
     gene            2331..2873
                     /locus_tag="VD737_08360"
     CDS             2331..2873
                     /locus_tag="VD737_08360"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXW10612.1"
                     /translation="MTVALRPVQVAYHVPDPVAAAERCARDYGWGPFVLLEHIALERS
                     LHRGTPRPFDHTSAYGQAGEVMVELITQHDDTPSALRDLYAADQSGVHHVACFVDDLR
                     AELQRFRDRGVVVALDARTKSGVDFAMVDLSRELGHMLELYEPGEDLRRFYAHVRKLA
                     DGWTGERPVRRPRTDTNTGG"
     gene            2879..3679
                     /locus_tag="VD737_08365"
     CDS             2879..3679
                     /locus_tag="VD737_08365"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXW10613.1"
                     /translation="MARLEGKSAVILGAAGRDNMGQVMARTFAREGARVLVAGRHEDE
                     LRAIADEIGGRHATCDITRKADVEALADAAAAAFGKVDVAVNCTGWGLMAKLLDTTEE
                     QLDRLAALQFKGPYFFLQAFVGRMAKSGGGSVVQVSSASVHCVLNNHAAYIATKAAGE
                     ALVRCFANEFGAHGVRVNSIAPGLTSTPMTEKEVRMPGLEEAFLKEYPLGRIGTTQDV
                     ADAALWLAGDECFMTGQVLQVNGGLTLRRNPTQKEISASLRAHAPAGK"
BASE COUNT          604 a         1256 c         1301 g          554 t
ORIGIN      
        1 atccaggagg tcaccgcatg ccgaccgccg tcgcgcgctc ccgcaagtcc acgaatcgcc
       61 cggccccgcc gtccacaccg gcggccccga aggcgaggaa gtcccgtgcg aaaaaccccg
      121 ccggcatcgc gctgctgccg cccgaggacc tggcgcggat gcggatccag gtcacgacga
      181 tcggcgacct gctgctgacc gcggcggatc gccatcccga atcgctcgcg ctggtgttcc
      241 cggaccgcac gatcacctat cgcgaactcg ccgacaacgc gctgcggcgc gcccgggcgc
      301 tgcaggccct gggcgtgcgg ccccgcgagc acgtcggcgt gctgatgcac acgtgcgccg
      361 agttcgtcga gacgtttttc gcgatttctt tctgcggcgc cgtgatcgtg ccgatcaacg
      421 cgcgctacaa gtcgagcgag ctcgcctacg tgatcgagaa cggcgacctc gtcaccgtgg
      481 tcacgaccga tgcggtcgcc gactcggtca acttcgtcga gcgcctgaac ggcgcgctga
      541 aggacctgcc gcgcagccgc aacgctcgcg aactgaagct ggccggcacg ccgaagctcc
      601 gtaacgtcgt gttgctgggc aagacgcgtg cgggcggtta cctgggagaa gccgagttcg
      661 agcaggccgc gcgcgaagtc gaggagtcgg aggtgcaccg cgcacggctg cgcgtgcgcg
      721 tgcgcgacgt cgggctgatg ctctacacgt cgggcaccac ggcgaatccg aagggctgcc
      781 tgatcacgca cgaggcgatg gtgcgcaaca gcatcgcgct cggccggcat cgctaccggc
      841 tcacgcacga cgaccgcatg tggtcgcccc tgccgatgtt ccacatcgcg gccgtgctgc
      901 cgatgctcgc gatcttcgac gtcggcggaa gctactgcac gatgggctac ttcgacgccg
      961 gcgtcgcatt gcagatgctc gagaagtacc gcgtcacggc gatgtacccg agcttcgtca
     1021 cgatcatgca gggcctcgtc taccacccga gcttcgcggg cgtagacctg tcgcgcgtca
     1081 agctgatgaa cagcaacttc gccgtgcagc cgcccggcgt cgccgagccg atcatgacgg
     1141 cgatgcccca ggcgctgcag gtcggcagct tcggcatgac cgaggcggcc ggaaccgtct
     1201 gtaccggcgg ctgggacgag gccgaggcgc gccgcatcac ccggctcgga cggccactgc
     1261 caggcctcga agtgcgcgtc atcaaccccg agacggggcg cgacctcaag aacggcttcc
     1321 gtggcgaggt gctcgtgcgc gggtactcgc tgttcgaggg ctactacaag gacgcggaga
     1381 agaccgcgca ggcactcgac gccgacggct ggttccacac cggcgacatc ggctcgctcg
     1441 acgatgacgg cacgatcatg ttccacggcc gcatcaagga catgctcaag gtcggcggtg
     1501 agaacgtcgc ggccgctgaa atcgaggggc tgctgcagcg acacccggcc gtcaagctcg
     1561 cgcaggtggt cggcattccc gatgccaagt acgtcgaagt gcccgctgcg ttcgtcgagc
     1621 tcaagcccgg gacgaaggcg ggtgagaagg agctgatcga gttctgccgc aaggagatct
     1681 cgagcttcaa ggtcccgcgc cacgtgcgct tcgtgaccga gtggccgatg tcgagcagca
     1741 agatccagaa gttccggctg cgccaggcgc tcgtcgccga gctcgggttg acctgacatg
     1801 gcaagcgcaa acccgcgaac cccgttcggc ccggtcatgc agctcggctt cgtggtgccg
     1861 gacctcgagc gcgccatgga gcactgggcc ggcacggtcg gcgtcggtcc gttcttcgtg
     1921 ctttcgcacg tccagttcgc cgagacgtac tacaagggcc agcccacccg gttcgacatg
     1981 tcggtgggtg tcgcccagtg gggtgcggtg caggtcgagc tgatccagca gttcgacgac
     2041 gtgccgtcga tctattcgga gtttccgggc cgcgcgctcg gcggattgca gcatctcggc
     2101 gtgatgacgc gatccgtggc ggacgatctc gagcgactgc gcggtcgcgg gatcgaaccg
     2161 gtgcagtggg gctccaccgc gaacggcatc cggttcgcct acgtgtcgac ggaccagcat
     2221 cccggcgcga tggtcgagct gatcgagcac ggcccggcga tcgacggttt cttcggcatg
     2281 gttcgcgagg ccgcacgcga ctgggacggc cgccaaccgg tgcgccgcct gtgaccgtgg
     2341 ccctgcgacc cgtgcaggtc gcctatcacg tgcccgaccc ggtcgcggcc gcggaacgct
     2401 gcgcgcgcga ctacggctgg ggcccgttcg tgctgctcga acacatcgcg ctcgagcgca
     2461 gcctgcaccg cggcacgccg aggccgttcg accatacatc cgcctatggc caggccggtg
     2521 aggtcatggt cgagctgatc actcagcacg acgacacgcc gtccgcgctg cgggacctgt
     2581 acgccgcgga tcagtccggc gtccaccacg tcgcgtgttt cgtcgacgac ctgcgcgccg
     2641 agttgcagcg cttccgcgac cgcggcgtcg tcgtcgcgct cgacgcgcgc acgaagagcg
     2701 gcgtggactt cgcgatggtc gacctgagcc gcgaactcgg tcacatgctg gagctgtacg
     2761 aaccgggcga ggacctgcgg cgcttctacg cacacgtgcg caagctcgcg gacggctgga
     2821 cgggcgagcg tccggtgcgc cgcccgcgca ccgacacgaa tacgggaggc tgacgacgat
     2881 ggcacgtctc gaaggcaaga gcgcggtgat cctcggcgca gccggccgcg acaacatggg
     2941 ccaggtcatg gcacgcacgt tcgcgcgcga aggggcgcgc gtgctggtgg ccggccggca
     3001 cgaggacgaa ctgcgcgcga tcgcggacga gatcggcgga cgccacgcca cctgcgacat
     3061 cacgcgcaag gccgacgtgg aggcgctggc ggacgcggca gcggccgcct tcggcaaggt
     3121 cgacgtcgcc gtcaactgca cgggctgggg gctgatggcg aagctgctcg acaccacgga
     3181 ggagcagctc gaccggctcg cggcgctgca gttcaaggga ccctatttct tcctgcaggc
     3241 gttcgtcgga cggatggcga agtcgggcgg cggctcggtg gtgcaggtct cgtccgcctc
     3301 ggtgcactgc gtgctgaaca atcacgcggc gtacatcgcg accaaggcgg ccggcgaggc
     3361 cctggtgcgt tgtttcgcga acgaattcgg cgcgcacggc gtgcgtgtca actcgatcgc
     3421 gccgggcctc acctcgacac ccatgaccga gaaggaagtg cggatgcccg ggctcgagga
     3481 ggcgtttctg aaggaatacc cgctcggacg catcggcacg acccaggacg tcgcggacgc
     3541 ggcactctgg ctcgccggcg acgagtgctt catgacgggc caggtgctgc aggtcaatgg
     3601 cgggctgacg ctgcgtcgca atccgaccca gaaggagatc agcgcgagcc tgcgcgcgca
     3661 cgcgccggcg ggaaagtagg ctgccctcgc ccgcgctcgt gcgggagagg tggag
//
LOCUS       DASPFR010000319         6973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_946988, whole genome shotgun sequence.
ACCESSION   DASPFR010000319 DASPFR010000000
VERSION     DASPFR010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6973
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946988"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /gene="ybeY"
                     /locus_tag="VD737_08370"
     CDS             <1..430
                     /gene="ybeY"
                     /locus_tag="VD737_08370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HXW10614.1"
                     /translation="GPVALRRWARAAYVAAGQAAPAPTLTVRVVGRAESRRLNRGFRG
                     KDQPTNVLSFPASPEERALGGALGDLVVCAPVVAREAREQGKAPSAHWAHMVVHGTLH
                     LLGHDHERPRQARAMEAVEVEILRGFGFDDPYLVVTAGRV"
     gene            427..1413
                     /locus_tag="VD737_08375"
     CDS             427..1413
                     /locus_tag="VD737_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019659043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HXW10615.1"
                     /translation="MSSNGHSGSSPNSRGWLRRIAESITGEPRDLEQLADVLNDARER
                     GLIDADVLEMLESVLDMSEIQVRDVMVPRSQMVVLNRDESVEKILPVVIESGHSRFPV
                     VGEDRDEVAGILLAKDLLRFFVEDDRDDFDIRECLRPAVFIPESKRLNVLLKEFRLTH
                     NHMAIVVDEYGGVSGLLTIEDVLEQIVGDIGDEYDVDEGEGIRKEADRTWTVPALTRI
                     EDFNETFGTRFSDEEADTIGGLVLHELGRMPRRGEAVQIGGLELKVTRADRRHIEMLR
                     VTTPEARPTHAPASPTVPLSSSTPAPSSTTSAPSSTTSAPPRTPRASGSKGG"
     gene            1418..2968
                     /gene="lnt"
                     /locus_tag="VD737_08380"
     CDS             1418..2968
                     /gene="lnt"
                     /locus_tag="VD737_08380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HXW10616.1"
                     /translation="MSAPDRLRRVLARGRWLALPLGLALAAAFAPLDLVPLAFVAPAA
                     LFVLWHDATPREAAWRGFLYTGGTFLAGTYWLYHSIHLVGQAPIGLALFLMLGLVAIM
                     GLYSALAGYAAARWFAPAGLVRWLLVLPALWVLAEWLRGWVLSGFPWLALGYTQLGTP
                     LRGYAPVLGVYGVSVAVAVSAGALAALVLGTRRERGIAVAVAGAVWLTGAGLTAVSWT
                     RPVGDAVSVALVQGAVPQSLKWQPEQRERTMRLYVELTRPHLGADIVVWPESAVPSLE
                     EHVRPYLERVSAAANARGSSLIMGLIRRDPATGAYFNSIAAWTGDAGSQPPQWYDKRR
                     LVPFGEFFPVPQTVRNWLRLMNLPYSDFQPGDDRQAPLRAGGHRLAPTVCYEDAYGSE
                     QLGLVRDSSLLVNVTNDAWFGDSTAPHQHLDISRMRSIESGRAMLRATNDGVTALIDH
                     DGAVLGRLPQFQPAVLTGHVQPREGATPYVRVGNWPIVLVSWLALAVAAWAWRRAAPG
                     GSAPGRRA"
     gene            2965..4053
                     /locus_tag="VD737_08385"
     CDS             2965..4053
                     /locus_tag="VD737_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXW10617.1"
                     /translation="MTEHRGAITREGELYPSPRQGLDYLDLEPPAPGHAVPLGANLYW
                     ARIPLPMELNHINVWLLDDEDGGWLLVDTGMSEDVCREAWVSLEQQLLQGRPLRNVLV
                     THDHPDHMGLAGWLAQRHGAPLWMSAIGHGSTLEFLATPGEDVDARRLRFVASHGMEI
                     SPDGARRTGGGTEHGRWYGAVPPLARAVAGGDRIEAGGRAWDVIETSGHCRGHLCLHD
                     AANRVLISGDQVLPTISPNVSVLATRPDSNPLAEFLESLARLERQCAPDTLVLPSHGR
                     PFRGLHRRIEVLRSHHLQQLDALRAACREPTAAYALLPVMYGRPLRGFHRFLAVGETV
                     AHLNHLWHDGAVERRVDEAGRVTFKASG"
     gene            4276..6861
                     /gene="leuS"
                     /locus_tag="VD737_08390"
     CDS             4276..6861
                     /gene="leuS"
                     /locus_tag="VD737_08390"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HXW10618.1"
                     /translation="MQERYEPSVLEPAAQRYWDDTGAFAVAEDPSRPKYYCLCMFPYP
                     SGRLHMGHVRNYTIGDVLTRFMRMQGHNVLQPMGWDAFGLPAENAAMANGVPPARWTY
                     DNIDYMKKQLKSLGLGIDWSREVATCRPEYYRWNQWLFLRMLERGIAYRTTGLVNWDP
                     VDQTVLANEQVIDGRGWRTGALVEKREIPMYYLAITRYAEDLLAALDHLPGWPERVKL
                     MQANWIGRSEGLEIEFDVEGGGTLGIYTTRPDTLCGVTYMAVAAEHPLAQRAAQSNPD
                     LQAFIAECQRTGVTEAALETMEKRGMPTGLHAIHPITGERIPIWVANFVLMAYGTGAV
                     MAVPAHDQRDYEFARRYGLRIHQVIRPTDGSLANIDDEAYLEHGFLINSGQFDGLDFD
                     EAFDAFAKLFEAQGRGRRRINFRLRDWGISRQRYWGCPIPLIHCADCGVQPVPDDQLP
                     VVLPEELVPDGSGNPLNRHARFLECQCPKCGGPARRETDTMDTFVDSSWYFFRYTSSG
                     NDRAMVDDRATYWMPVDQYIGGIEHAILHLLYSRFWTRVMRDLGLTEVEEPFQNLLTQ
                     GMVLNEIYLRKPAEGRITYYNPADVDVQVDGKGARTGAVLRADGLPVESAGIGTMSKS
                     KNNGVDPQSLVERYGADTARLFMMFAAPPEQTLEWSDEGVEGAFRFMKRLWKAVHTHV
                     QAGLPTEPLHRHALSPAQRDIRRQVHETLAKITHDIGKRRTFNTAIAAVMELMNALAK
                     FGDDSAQGRAVMQEALEIVVLGLSPVVPHACHALWHALGHERAVVDERWPALDPEALV
                     REAIEVVVQVNGKLRGRVTVPANADEAQVREAALADEGVQRYTDGKPIRKFVYVPGKL
                     ANVVV"
BASE COUNT         1025 a         2433 c         2465 g         1050 t
ORIGIN      
        1 agggccggtc gcgctgcggc gctgggcgcg cgcggcgtac gtcgcggccg gccaggccgc
       61 gccggcgccg acgctgacgg tgcgcgtggt ggggcgcgcg gaaagccggc ggctcaatcg
      121 cgggtttcgc ggcaaggacc aaccgaccaa cgtgctgtcg tttccggcct cgccggagga
      181 gcgcgcgctg ggcggcgccc tcggcgacct cgtggtctgc gcgccggtgg tggcgcgcga
      241 ggcgcgcgag cagggcaagg cgccgtcggc gcactgggcg cacatggtcg tgcacggcac
      301 gctgcacctg ctcggacacg accacgagcg gccgcgacag gcgcgggcca tggaagccgt
      361 cgaggtggaa atcctgcggg gtttcgggtt tgatgacccc tatctcgtgg tcactgccgg
      421 cagggtatga gtagtaacgg gcacagcgga tcgtcgccga attcccgcgg ctggctgcgt
      481 cgcatcgccg agtcgatcac cggcgagccg cgcgacctcg agcagctcgc cgacgtgctc
      541 aacgacgccc gcgaacgcgg gctcatcgac gccgacgtgc tcgagatgct cgagtcggtg
      601 ctcgacatgt ccgagatcca ggtgcgcgac gtgatggtgc cgcgctcgca gatggtcgtg
      661 ctcaaccgcg acgaatccgt cgagaagatc ctgccggtcg tgatcgaatc gggccactca
      721 cgctttccgg tcgtcggcga ggatcgcgac gaggtcgccg gcatcctgct cgccaaggac
      781 ctgctgcggt tcttcgtcga ggacgaccgg gacgacttcg acatccgcga gtgcctgcgc
      841 cccgcggtgt tcatccctga aagcaagcgc ctcaacgtgc tgctgaagga gttccgcctc
      901 acccacaacc acatggcgat cgtcgtcgac gagtacggcg gcgtgtccgg cctgctcacg
      961 atcgaggacg tgctcgagca gatcgtgggc gacatcggcg acgagtacga cgtcgacgag
     1021 ggcgagggca tccgcaagga ggccgatcgg acgtggaccg tgccggcgct gacgcggatc
     1081 gaggacttca acgagacgtt cggcacgcga ttctcggacg aggaagcgga cacgatcggc
     1141 ggcctggtcc tgcacgaact cggccgcatg ccgcgccgcg gtgaggccgt gcagatcggc
     1201 ggcctcgaac tcaaggtcac gcgcgccgac cggcgccaca tcgagatgct gcgcgtgacg
     1261 acgccggagg cccggccgac gcacgcgccg gcgtcgccca ccgtaccgtt gtcgtcctcg
     1321 acgcccgcgc cgtcgtccac cacgtccgcg ccgtcgtcca ccacgtccgc gccgccgcgg
     1381 acaccacggg cgtccgggtc caagggcggc tgaccgggtg agcgcacccg atcgattgcg
     1441 acgcgtcctg gcacgcgggc gctggcttgc gctgccgctc ggcctcgcgc tcgccgcggc
     1501 cttcgcgccg ctcgacctgg tgcccctcgc gttcgtcgcg ccggctgcgc tgttcgtgct
     1561 gtggcacgac gcgacgccgc gcgaagccgc ctggcgcggc ttcctgtaca ccggcggcac
     1621 gttcctggcc ggcacctact ggctgtacca cagcatccac ctggtggggc aggcgccgat
     1681 cgggctcgcg ctgttcctga tgttggggct ggtcgccatc atgggcctct actcggcgct
     1741 cgcgggctac gcagcggcgc gatggttcgc gccggccggc ctggtgcgct ggctgctggt
     1801 gctgccggcg ctctgggtgc tcgccgagtg gctgcgcggc tgggtactga gcggattccc
     1861 gtggctggcg ctcggctaca cgcagctcgg caccccgttg cgcggctacg cgccggtgct
     1921 cggcgtgtac ggcgtgagcg tcgccgtcgc cgtctcggcg ggcgcactgg ccgcgctcgt
     1981 gctcggcacc cgccgcgaac gtgggatcgc cgtcgcggtg gccggggcgg tgtggctcac
     2041 cggcgccggc ctcaccgccg tttcctggac ccgccccgtc ggcgacgcgg tcagcgtcgc
     2101 gctcgtgcag ggcgccgtgc cgcagtcgct caagtggcag cccgagcagc gcgagcgcac
     2161 gatgcggctc tacgtcgaac tcacccggcc gcacctcggt gccgacatcg tcgtgtggcc
     2221 cgagtccgcg gtgccgtcgc tcgaggagca cgtgcgacca tacctcgaac gggtctcggc
     2281 tgccgcgaac gcccgcggct cctcgctgat catgggcctg attcgacgcg atccggcgac
     2341 cggagcgtat ttcaactcga tcgcggcctg gacgggcgac gcgggttcgc agccgccgca
     2401 gtggtacgac aagcgccggc tcgtgccctt cggcgagttc tttcccgtgc cgcagacggt
     2461 gcgcaactgg ctgcggctga tgaacctgcc gtactccgat ttccagcccg gcgacgatcg
     2521 gcaggcgccg ctgcgcgcgg gcggacaccg gctcgcaccc accgtctgct acgaagacgc
     2581 ctacggatcc gagcaactcg ggctggtgcg cgactcgtcg ctgctcgtca acgtcaccaa
     2641 cgatgcgtgg ttcggcgact cgaccgcgcc gcaccagcac ctcgacatca gccgcatgcg
     2701 ctcgatcgaa agcggtcgcg cgatgctgcg cgcgaccaac gacggcgtga ctgcgctgat
     2761 cgaccacgac ggcgccgtgc tcggtcggct gccgcagttc cagccagccg tgctgaccgg
     2821 ccacgtgcag ccgcgcgagg gcgccacgcc gtacgtgcgc gtcggcaact ggccgatcgt
     2881 gctggtgtcg tggctcgcgc tggcagtcgc cgcgtgggcg tggcgccgcg ccgcgcccgg
     2941 gggatccgcg ccggggcgac gcgcgtgacc gagcatcgcg gcgcgatcac gcgcgagggc
     3001 gagctctacc cctcgccgcg gcagggactc gactacctcg acctcgagcc gcccgccccg
     3061 ggccacgccg tgccgctcgg cgcgaacctg tactgggcgc gcattccgct gcccatggaa
     3121 ctgaatcaca tcaacgtctg gctgctcgac gacgaagacg gcggctggct gctcgtcgac
     3181 accggcatgt cggaggacgt ctgccgcgag gcatgggtgt cgctcgagca gcaattgctg
     3241 cagggccggc cgctccgcaa cgtcctggtc acgcacgacc accccgacca catggggctc
     3301 gccggctggc tcgcgcaacg gcacggcgcg ccgctctgga tgtccgcgat cggccacggt
     3361 tcgacgctcg agtttctcgc gacgccgggc gaggacgtcg acgcgcgtcg attgcgcttc
     3421 gtggcctcgc acggcatgga gatctcgccc gacggcgcgc gccgcacggg aggcggcacc
     3481 gaacacggcc gctggtacgg tgccgtgccg ccactcgcac gagcggtggc cggtggcgac
     3541 cgtatcgagg ccggtggtcg tgcctgggac gtcatcgaga ccagcggtca ctgccgcggg
     3601 cacctctgcc tgcacgatgc cgccaatcgc gtgctgatca gcggtgacca ggtgctgccg
     3661 accatctcgc cgaacgtgag cgtgctggcg acgcgacccg actcgaaccc gctcgccgag
     3721 tttctcgagt cgctcgcgcg gctcgagcgg cagtgcgcgc cggacacgct ggtgctgccc
     3781 tcgcacggcc ggccgttccg aggactgcac cggcgcatcg aggtcctgcg ctcgcatcac
     3841 ctgcagcagc tcgatgccct gcgcgcggcc tgccgcgagc ccaccgctgc ctatgcactg
     3901 ctgccggtga tgtacggccg accgctccgc gggtttcacc ggttcctcgc ggtcggtgag
     3961 accgtcgccc acctgaacca cctgtggcac gacggggcgg tcgagcggcg ggtggacgag
     4021 gcggggaggg tgaccttcaa ggcgagtggc tagtggtcag tggtcagtgg ctagtggcta
     4081 gtggctagtg gctagcggtc agtggtacgg attcccccag gcgcgggatc ctgcagcctg
     4141 gacatcgacg ccgaaggcag ctccccacta gccactagtc actagccacc gcaaagtcct
     4201 ccccggatgc cgtccgccgc gccgctccag tatcctgtgc aatccgccca cgccccgacc
     4261 agcgatcgat ccgtcatgca ggaacgctac gaaccgtccg tgctcgagcc tgcggcccag
     4321 cgctactggg acgataccgg cgccttcgcg gtggccgagg atccttcgcg gccgaagtac
     4381 tactgcctct gcatgttccc ctatcccagc ggtcgcctgc acatggggca cgtgcgcaac
     4441 tacacgatcg gcgacgtgct gacgcgcttc atgcgcatgc agggccacaa cgtgctgcag
     4501 ccgatgggat gggacgcgtt cggactgcct gccgagaacg cggcgatggc gaacggcgtg
     4561 ccgcccgcgc gctggacgta cgacaacatc gattacatga agaagcagct gaagtcgttg
     4621 gggctgggca tcgactggtc gcgcgaagtc gcgacctgcc gccccgagta ctaccgctgg
     4681 aaccagtggc tgttcctgcg catgctcgag cggggcatcg cgtaccgcac caccggcctc
     4741 gtgaactggg acccggtcga ccagaccgtg ctggccaacg agcaggtgat cgacgggcgt
     4801 ggctggcgca cgggcgcgct ggtcgagaag cgcgaaatcc cgatgtatta cctcgcgatt
     4861 acgcgctatg cggaggatct gctcgccgcc ctcgaccacc tgccggggtg gcccgagcgc
     4921 gtcaagctga tgcaggcgaa ctggatcggc cgctccgagg ggctcgagat cgagttcgac
     4981 gtcgagggcg gcggtacgct cggcatctac acgacgcggc cggacacgct ctgcggcgtc
     5041 acgtacatgg ccgtggcggc cgagcatccg ctcgcgcagc gtgccgcgca gtccaatccc
     5101 gacctgcagg cattcatcgc cgagtgccag cgcacgggcg tgaccgaagc cgcgctcgag
     5161 acgatggaaa agcgcggcat gccgacaggc ctgcacgcga ttcacccgat cacgggcgag
     5221 cggataccga tctgggtcgc aaacttcgtg ttgatggcgt acggcacggg cgccgtgatg
     5281 gccgtgcccg cgcacgacca gcgcgactac gagttcgcgc gccgctacgg cctgcgcatc
     5341 caccaggtga tccggccgac cgacggctcg ctggccaaca tcgacgacga ggcctacctc
     5401 gagcacggct tcctgatcaa ctccggccag ttcgacggcc tcgatttcga cgaggccttc
     5461 gacgccttcg ccaagctgtt cgaggcgcag ggtaggggcc ggcgacggat caacttccgg
     5521 ctgcgcgact ggggtatctc gcgccagcgc tactggggct gcccgatccc gctgatccac
     5581 tgcgccgact gcggcgtgca gccggtgccc gacgaccagt tgccggtggt gctgccggag
     5641 gaactcgtgc ccgacggcag cggcaatccg ctcaaccggc acgccaggtt cctggagtgc
     5701 cagtgcccga agtgcggcgg ccccgcgcgg cgagagacgg acacgatgga cacgttcgtc
     5761 gactcgagct ggtatttctt ccgctatacg agctcgggca acgaccgcgc gatggtcgac
     5821 gatcgcgcga cgtactggat gccggtcgac cagtacatcg gcggcatcga gcacgcgatc
     5881 ctgcacctgc tgtattcgcg cttctggacg cgcgtgatgc gcgacctcgg cctgaccgag
     5941 gtcgaggagc cgttccagaa cctgttgacg cagggcatgg tgctgaacga gatctacttg
     6001 cgcaaacccg cggagggccg catcacgtac tacaacccgg ccgacgtcga cgtgcaggtg
     6061 gacgggaagg gcgcgcgcac cggcgccgtg ctgcgtgccg acggcctccc cgtggaatcg
     6121 gccggcatcg gcacgatgtc gaagtcgaag aacaacggcg tcgacccgca gtcgctggtc
     6181 gagcggtacg gcgcggacac cgcgcggctg ttcatgatgt tcgcggctcc acccgagcag
     6241 acgctggagt ggagcgacga aggcgtcgag ggcgccttcc gcttcatgaa gcggctgtgg
     6301 aaggccgtgc acacgcacgt gcaggcgggt ttgccgaccg agccgctgca ccgccacgcg
     6361 ctctcgcccg cacagcgcga catccgccgc caggtgcacg agacgctcgc gaagatcacc
     6421 cacgacatcg gcaagcgccg cacgttcaac acggcgatcg cggcggtcat ggaactgatg
     6481 aacgcgctcg cgaagttcgg cgacgacagt gcgcagggcc gcgcggtcat gcaggaggcg
     6541 ctcgagatcg tcgtgctggg tctgtcgccg gtcgtgccgc acgcctgcca cgcgctgtgg
     6601 cacgcgctcg gccacgagcg cgcggtcgtc gacgaacgct ggccggcgct cgacccggaa
     6661 gcgctggtgc gcgaggcgat cgaggtcgtc gtgcaggtca atggaaagct gcgcggccgc
     6721 gtgacggtgc ccgcgaacgc ggacgaggcg caggtgcgcg aagcggcgct cgccgacgag
     6781 ggcgtgcagc gctacacgga cggcaagccg atccgaaagt tcgtgtacgt gccgggcaag
     6841 ctcgccaacg tggtggtgtg aggccgtgag gtcttccttg gcgccgtcgc ggttgggccg
     6901 gatcgtcacg cgcgttcggc gtgccgcggg gcgcatcgcc tcgacggttt ggcgccggag
     6961 tctgtcgctg tcg
//
LOCUS       DASPFR010000320        23521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_582250, whole genome shotgun sequence.
ACCESSION   DASPFR010000320 DASPFR010000000
VERSION     DASPFR010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 23521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23521
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582250"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VD737_08395"
     CDS             <1..1073
                     /locus_tag="VD737_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXW10619.1"
                     /translation="PKGVLYSHRSTVLHAYGGCMADSLGLSGRDCVLAVVPMFHANAW
                     GLPYNAPMTGAKLVFPGPRMGDAPTLTQLMNDEGVTLAAAVPTVWTMLLNHLQQTGEK
                     LTSLERTVIGGSAVPLQMIRDFRDRHGVTVIQGWGMTETSPLGSLSTLRPEQLQLPPE
                     EQVLLRGKAGHGIFGISMKVVDEQGRELPWDGQSAGELKVSGPWVCSGYFRLADSPAH
                     REPGWFATGDVAMIHPNGFLQITDRAKDVIKSGGEWISSVDLENCACGHPEVLMAAAI
                     GVRHPKWDERPVLLVVPRQGCTPPSESILEHLGRHFAKWQLPDAIVLVEALPLTATGK
                     INKRSLRDTYGQVLVAPETQAS"
     gene            1208..2149
                     /locus_tag="VD737_08400"
     CDS             1208..2149
                     /locus_tag="VD737_08400"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXW10620.1"
                     /translation="MLRMDPSTLRRAAEQLKQTTEEHASWHEDVLRAIFCDELASADG
                     VLSGTAHRDCSFGRWFYEDAPNGLREQPAFVAVGKEHQRLHQVAAKLLRAAKGGAPVA
                     RADFEDFVATTARLRVQTEYLRSSIDAALGNRDPLTGAHGRIAMLPDLHELRAALQHG
                     GRPCALVFMDVDHLKHINDEHGHAAGDAVLCGVVAHVQEHLRSQDRVYRYGGDEFVLA
                     LPGADLDVAYAVATRMRDGIDDKLFVAGAGGAPVHVSASFGLALLDADEPVTESINRA
                     DQALLLAKTAGRSRVIKWDAAVTTSTRWRKIDADTER"
     gene            complement(2163..3134)
                     /locus_tag="VD737_08405"
     CDS             complement(2163..3134)
                     /locus_tag="VD737_08405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HXW10621.1"
                     /translation="MTDDPSRDHGATRMAATAGGLLSDTGPALVLPAAVALWLPRLDA
                     LPPADPRDVLLALEIDRHALIVHLHGAEAAEVIERHLHGALEERSPEASGSLVRTGPG
                     QCAVLARGPLEAVETLARSAAEHFGRRSWSGLGETTVSAGLARRFPGEPGGSWWSRVE
                     SALAQAKAGGGGQVVIDRRSTADDGSVGAPGLHLQWQARFECGEPTIDRQHRELFARA
                     EDVLEALRKGSSRFATDLERLMDEIARHFADEELILKQRGYRGLSRHRRSHAMLSAKA
                     MRLKAEAAAGRASREELTRFLLGEVVADHMLTEDRRFAELFASAHHR"
     gene            complement(3247..4494)
                     /locus_tag="VD737_08410"
     CDS             complement(3247..4494)
                     /locus_tag="VD737_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXW10622.1"
                     /translation="MMHRINPVDTPMLFQLRLLAYRLLKPFLWPVSRLVAIPRPTLFV
                     GPGAAQRLCTSLGQFGLRRVMIVTDAVLVKLGLVEPLKRALEAQGINVAVHDGITPDP
                     TYPVLQAGYEAVKAHGSDAILAVGGGSAIDAAKVIDAMVVTGKSPARLIGMLKVSKPM
                     LPLYAIPTTAGTGSEVTVAAVVTDPVAHVKSAVIDPKLVPLGAALDPELMRGMPKPIT
                     AATGMDALTHAVEAYVNRWPHAETAQHCLAAVRLIFGNLQRAYDDGNDLAAREAMALA
                     SFYAGLAFTKAYVGYVHAFSHKIGGLYGVPHGLANAITLPYVLDFLVDEPRTRDRLAE
                     LAVAIGAGVAGEPPEVLAQRFVSRVRALNRAVGIPEKMAALRPDDVPEIARAAMVEAS
                     RDYPVPKVMALAESEALLRRMQA"
     gene            complement(4624..5133)
                     /locus_tag="VD737_08415"
     CDS             complement(4624..5133)
                     /locus_tag="VD737_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10623.1"
                     /translation="MSAEQVVQAYFAGEKAEAFWILLAGLAAGAAAVTLWWWVREPFA
                     RGLALALVVVAALGLGVGGTVYFRSDDQARRLVQLHAADPGQFSAEEGPRIAQVVRSF
                     GWYRYAYGVATLLALVLVFAFGRPSHHGVAVGLLLLAALGFTIDFYAERRAIDYQQAL
                     TRHGWLAPR"
     gene            complement(5150..6685)
                     /locus_tag="VD737_08420"
     CDS             complement(5150..6685)
                     /locus_tag="VD737_08420"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXW10624.1"
                     /translation="MHTGGAAQREASLAFAEYGAHDALGLAALVRAGEVSASELLDEA
                     LSRTAAVNPRINAVIHLMERNARAAIAAGLPDGPFSGVPFLIKDLMTAYAGEPMRSGS
                     RLFRDFVPPADEELTRRYRAAGVVIFGKTNTPEFGVSNVTEPELFGPTRNPWNLDRSP
                     GGSSGGSAAAVAARIVPAANANDGGGSIRTPASNCGLVGLKPSRGRNPTGPQLPDVWF
                     GFIGEHVVTRSVRDCAALLDATCGDYPQQLMKLPPPTRPYLDETQAPAGRLRIAYSFD
                     PGLGHAMHPENRRALESAVGQLTALGHELVEVHLPLPREVFVAQYASLIAAEVAATLR
                     LAPALVGRKPRSDDLELTTRVLAKLGEAQAGAEVTAALLAMQAFARQWLAWADAFDVL
                     LTPSVGVPPLPIGAYQLPAARRAALRALVALPGAALKSQRTKVLEAFAPVFDAAPFTM
                     VANVTGQASMSLPLHWTDDGLPMGLLFTASRIGDEATLFRLAAQLETAVPWAHRIAPH
                     AIP"
     gene            6861..9014
                     /locus_tag="VD737_08425"
     CDS             6861..9014
                     /locus_tag="VD737_08425"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HXW10625.1"
                     /translation="MARTYCRRPLTSLVFALATVLTGPSLAGAAPVTAERILAADREP
                     QNWLAHGRTYGEQRFSPLTQVNDRNVAQLGLAWSYATGTNRGLQATPIVVDGRLYATG
                     VWSVVYALDAKTGQELWRYDPQVPRAWGRYACCDAVNRGVALWGDSVFVGTLDGRLLA
                     LDAKTGKKRWEVLTIDPTKPYTITGAPRVVKGKVLIGNGGGEYGVRGYFSAYDAKTGK
                     LAWRFYTVPGNPRDGFETPELARAAKTWTGEWWVGGGGGTVWDSMAYDAELDTLYVGT
                     GNGSPWARSIRSPGGGDNLFLSSILAIDPDDGALKWHYQVTPADNWDYTATQHIVLAD
                     LTFAGKPRKVLMQAPKNGFFYVIDRVTGELLAADKYIPATWASHVDLATGRPVETTEA
                     DWSRTTRLIIPAAFGGHNWHPMAYNPGTGLVYIPAMQPMGIYTPSREYLETGKYTRRE
                     MFWNPGVDWNEYTKSIYGLMQQFGGALPPDRGYLKAWDPVARRTRWEIEHPAFWNGGV
                     LTTAGNLVFQGTGDGRFVAYAADTGRTLWTMTTQVGIVAPPVTYAVDGEQYVAVLVGY
                     GGAGAVTGGDPRTMASGKYVNDGHVLAFRLGGTAPMPRVAERDQDIPEPPAIEATPEQ
                     IERGKYAYTANCMVCHGALAVSGGVTPDLRRLSAEKHSIFKQIVYDGVIHTAGMPRMS
                     DLVSEQDVRDIQAYIVQRAREDRAAGQKAAPARKP"
     gene            9163..9639
                     /locus_tag="VD737_08430"
     CDS             9163..9639
                     /locus_tag="VD737_08430"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXW10626.1"
                     /translation="MSTAPNPVPPGMHSVTPHLVCDGAAKAIEFYEKAFGATSSGAMK
                     GPDGRVMHAQLRIGDSHVMLVDEYPEQGMLGPRALKGTPVTMHLYVDDADRWFERAIA
                     QGAKTVMPLADMFWGDRYGLLEDPFGHRWSIATHKRDVPPEEMQQAVKNLDPTCGK"
     gene            9678..10040
                     /locus_tag="VD737_08435"
     CDS             9678..10040
                     /locus_tag="VD737_08435"
                     /inference="COORDINATES: protein motif:HMM:NF018893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HXW10627.1"
                     /translation="MGIYVDGFVLPVPTKRLAEYRKMARLAGKIYKELGVLAYVEAVA
                     DDVTPGKWTSFPRSVKLKPGETVVFSYIVHRSRKERDRVNKKFMSDPRLKKYMDPKAM
                     PFDGKRMFWGGFKSLVEL"
     gene            complement(10076..10693)
                     /locus_tag="VD737_08440"
     CDS             complement(10076..10693)
                     /locus_tag="VD737_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HXW10628.1"
                     /translation="MQTTPEPPTPPCRRLDLPDADVRYWPHALGAARADVLLASLRAG
                     IDWQQEHVVIFGTPRLVPRLVAWHGDPDAHYTYSGTAHEPHAWTDELRELREVAEALT
                     GHRYNSVLLNLYRDGRDGMGWHADDEPELGRDPAIASLSLGATRRFRLRHRRSRQGID
                     IDLVHGSLLSMAGETQHHYVHALPKTARTVGERVNLTFRWVTPSE"
     gene            complement(10647..11021)
                     /locus_tag="VD737_08445"
     CDS             complement(10647..11021)
                     /locus_tag="VD737_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HXW10629.1"
                     /translation="MRLGELLRRRPEAATVHVEPFGWEFRVEPGQTLLQAALAHGLPW
                     PSRCRVGSCTTCRCRLLEGQVRALTDSSYVLTKQQLAERVILACQSQPRGIVRVHLER
                     TKATLRADADDSGTPDAAVPAA"
     gene            complement(11018..12187)
                     /locus_tag="VD737_08450"
     CDS             complement(11018..12187)
                     /locus_tag="VD737_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HXW10630.1"
                     /translation="MGRYLKFLAVHGFVLATTLGIALGGAWMWLGIGVIYAVMIAGDE
                     WIGDDYAEPAYAQPRLLDALLYLALPTLGLLCCVMAWMAGSGDLFGLGAWVQAQAGYD
                     LFAARAATGPLHWLGGILSAALTFAAVGTNGGHELTHRTADRGAMIAGRWLLALTFDA
                     EFAIEHVYGHHRRLATPADPATARRGEHFYAFLVRSIAGQTRSAFRLERDRLAKLDLP
                     TWSWHNRYLRGWGMSGLIVAAFGLAAGAAGVVVFVLAGLAGRTVLEAVNYFEHYGLVR
                     EPTSPIEPRHSWNSNKALSNVVLFNLARHSHHHAEGDAPFWRLRSYRDAPTLPYGYLT
                     CMLLVYVAPGWYRDRMTPLVLDWDRRYANATERVLAAAANRASGLVALQATQAAA"
     gene            12303..12968
                     /locus_tag="VD737_08455"
     CDS             12303..12968
                     /locus_tag="VD737_08455"
                     /inference="COORDINATES: protein motif:HMM:NF025338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXW10631.1"
                     /translation="MGFSTMPRRRPTEPTDTRERIADAALSLFNADGTPAVSTRHVAA
                     RLGISPGNLYYHFGNKEEIVLHLYERIETELQAILAPPAHDARTFEEVLGYLDAIFAH
                     LWKFRFFYRDLNSLLRDVPGLKERYRRLAERVLARSKAIFTEMVQHGWMEASDEHLDL
                     LVTNAWIVLTQWFTHRQVVERRRTIQSSDVREGIRHLVALFSPLLRRPQRRQIEELLA
                     QDA"
     gene            13053..14063
                     /locus_tag="VD737_08460"
     CDS             13053..14063
                     /locus_tag="VD737_08460"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXW10632.1"
                     /translation="MLTPSIRARLTNALLRLTVKRLWRPDIAIDEVRRHAARSDVRLS
                     GRPPRCPVEAVEIAGLRAHWFGAPELAARHGTLLYLHGGAWCMHLPRLYAAFAAALSN
                     ATGLRVLLGEYRLAPEHPFPAGMDDCLATYRALVDGPHGTPVAIAGDSAGGSLSLITL
                     MRARDAGLPLPACAVLLSPSTDLTFGGPSMRYNALADPMFSEASMDLLPDIYCPGQDR
                     GNPLLSPLFGDWSGLPPLYFLAGSTEMLLDDSVRAHDRALQAGTSSRIDVWYQLPHVF
                     PVMHRLPESRVALRAIAAFIDQHRALPRGLVPPVVDPLVAAVAQVGMASVEASPGGRS
                     SP"
     gene            complement(14067..14303)
                     /locus_tag="VD737_08465"
     CDS             complement(14067..14303)
                     /locus_tag="VD737_08465"
                     /inference="COORDINATES: protein motif:HMM:NF021362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HXW10633.1"
                     /translation="MNQALLKTFTFALLHFGVAFSVAYLLTGSIAVATGVGLIEPIVN
                     TVAFHFHERAWRRAEARRAERPVLARLHAEPVRR"
     gene            14501..15259
                     /locus_tag="VD737_08470"
     CDS             14501..15259
                     /locus_tag="VD737_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXW10634.1"
                     /translation="MQGEPLAAPVPDRHVAQTPALTLRARGLTKTYGAGELAVHALRG
                     IDLELATGELAVLLGPSGSGKSTLLNILGGLDTATSGEAWFLDHDLTRASDRELTRYR
                     REHVGFVFQFYNLIPSLTALENVALVTEIATAPMDPAVALRLVGLGDRVHHFPAELSG
                     GEQQRVAIARAIAKQPAILLCDEPTGALDSKTGVLVLQAVESIHRELGTTTVLITHNA
                     VIAEMADRVIVLGDGMIQQARRNPRRRAASELAW"
     gene            15259..17622
                     /locus_tag="VD737_08475"
     CDS             15259..17622
                     /locus_tag="VD737_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXW10635.1"
                     /translation="MRALDRKLSRDLWRLRGQVLAIGMVIASGVALLVMSLTSIEALT
                     ETATAYYERYRFASVFARVERAPEHVAARLAAIEGVQSVTTRVVRSAVLEVEDFAEPV
                     IGQLISVPERGEPPLNALAMRQGRYPRETAPDEVVLSEPFAQAHHLALGDHVQAVLNG
                     RWRRLTVVGVALSPEYVYTIGPGALMPDDRRFGVLWMGERALQAAFDLEGAFNDVSLG
                     LLRGADPDAVVDRVDRMLARYGSIGAYSREDQLSNWFLMNEIAQLRTMARLLPTVFLA
                     VAAFLTNMVLARLVAVERSEIGLLKAFGYSGGEIGWHYVKLVLVIGAVGIALGWFLGY
                     WLGLYNTVTYAEFYKFPFLLYRPGPGPFAIAALASLAAALLGTIRAVRDAASLPPAQA
                     MQPPLPTRFHHGLLARWSALRSLDQPSRIVLRQVARWPVRSFVASIGIGLAIAVLIGS
                     MQWLDAIDHIEDVYFLQAQGQDVTVGFSQAQPADAARTLGRLPGVMATEPVRAVAARL
                     CAGPRMQREAVQGVPAHPQLSRVYDASGRAVELPREGLVISTMLAQLLKVGRGDTIVV
                     EVLEGRRPVFEARVAEVFETYIGSPAYMEIGALARLLQEPPSVTGVHLRIDPVEQARF
                     FRTLKDVPRVSAVTLREAAIATYRETMARTLLIFVSFFVVFSCALAFGVTYNTARIAL
                     SERGRELATLRVLGFTRAEISYILVGETGVLTLAALPLGLACGYALARALVAGLQTEL
                     FRVPFVISAATYGWALLIGIAATVASVLLVRRRLDRLDLVAVLKTRE"
     gene            17619..18845
                     /locus_tag="VD737_08480"
     CDS             17619..18845
                     /locus_tag="VD737_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HXW10636.1"
                     /translation="MKELFMQAALRKRLVLWIPPLALVLIALAWLLRPPAAAVDLAEA
                     RHGPLRVSVSDDGETRVRDVFVVSTPVTGLMRRIELEAGDAVVAQETVVARIEPSDPA
                     FLDRRSEGEAVAALRAAEAARTHAEAEVRRVEAELEFAQSELRRYEGLVRRAAISEND
                     LDAARRRARTAEAALQEARAGLRVRASEVEQARARLRAPGAPRDGDCACVVVRSPVSG
                     QVLRVLIESEGVVAVAAPLIEVGDPQQLEIVVDLLSTEAVRVAPGQKVLVEGWGGPQP
                     LEAVVRRVEPFGFTKVSALGVEEQRVNVVIDFTGEPAQWSRLGHGFRVEPRIVLWEAS
                     DVLQVPLSALYREGSTWRVFREVDGRARATDVRIGHDDGLEVEVLDGLQPGERVVVHP
                     SDRVEEGTRIVQRQLR"
     gene            complement(18855..19283)
                     /locus_tag="VD737_08485"
     CDS             complement(18855..19283)
                     /locus_tag="VD737_08485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HXW10637.1"
                     /translation="MCALLGAALALAALPAAPEAAGGLALDRTVLRGFSAVQEGLVSW
                     YGAAFHDRPTASGERFDSDGYTMAHPTLPFGTAVKVTNLRNGRSVVVRVNDRGPHVGQ
                     RIADLSRAAAAQIGMLRRGVVRARIEVVGDPAEPRSVAQR"
     gene            complement(19422..19745)
                     /locus_tag="VD737_08490"
     CDS             complement(19422..19745)
                     /locus_tag="VD737_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10638.1"
                     /translation="MRSLLIFAAVGVLAGCASQNTAAPPPATTYAATDVPEATTLEEA
                     QKLGYKIVNEDGKTMYCRETRKLGSHVQKERTCLTEAEMYEARNSNQRQFEDMKKVIG
                     QRKGN"
     gene            20103..>23521
                     /gene="gltB"
                     /locus_tag="VD737_08495"
     CDS             20103..>23521
                     /gene="gltB"
                     /locus_tag="VD737_08495"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HXW10639.1"
                     /translation="MTHTPHDHGDLATGTRAGLGDGPAQLGPPSKQGLYDPRHEHDAC
                     GQGFVVDIKGRKSHRIVEQAIQVLKNLDHRGACGSEVNTGDGAGILIQMPHAFNEEVC
                     RKARIQLPGPGEYGSGIIFLPRNPTVRRQVEQRFEQVVQSEGLHVLGWRTVPTSNGML
                     GETARSCEPFMRQVFIGRPAELTDDLAFERKLYVVRKRAYNDIRTSTIAGAEYWYVVS
                     LSARTFVYKGMLLTTQLDQYFHDLQHPLLESALALVHSRFSTNTFPSWDRAHPYRYIA
                     HNGEINTVRGNSNWMHAREARFECEAFGDDIRKIRPIINPNGSDSGMFDNTLELLYLS
                     GRSMPHAVMMMIPEPWSNHESMDDGKRAFYQYHSSLMEPWDGPASISFTDGTLIGAVL
                     DRNGLRPGRYYVTNDDLVILASEAGVLDVPPETVVRKGRLQPGRMFLVDTAQGRIIED
                     EEIKRTLATARPYREWLDEHLIHVDDLPQPPELPPSDPDSLLQRQIAFGYTFEDQRVV
                     LTPMARDGVEAVGSMGNDTPLAVLSARPRLLYDYFKQLFAQVTNPPIDCIREEIITSA
                     ETRLGSEGNLLNPQPSDCRRLELKWPLVTNEEFARIRRMDLPGLRVGVLPTLFRVSRG
                     EKGLVKSMEELRLMARRLIEEEEVNVIVLSDRGVNKDFAPIPALMAVAGLHHYLIREG
                     LRTRVSLVLETGEAREVHHFAVLIGYGVSAINPYLAFETIDQMIKEGLLTNIDHKTAC
                     RNFVKAAAKGVIKVASKMGISSLQSYRGAQVFEAVGLRQDVIDEYFSWTASRVGGIGT
                     DVIAQEVLLRHNAAFPERRGLAAHTLPVGGQYQWRDDGEQHLFNPESIHRLQKAVRTG
                     SYETFKSYSALVNGLSSNLCTLRGLLEFKGAEPVPLEEVEPVESLMKRFKTGAMSYGS
                     ISKEAHETLAIAMNRIGGKSNTGEGGEDPERYRWTNEHGDSKNSAIKQVASGRFGVTS
                     EYLVQARELQIKMAQGAKPGEGGQLPGSKVYPSIAKTRHTTAGVGLISPPPHHDIYSI
                     EDLAELIHDLKNANRRARISVKLVAEVGVGTIAAGVAKAHADVVLISGYDGGTGASPQ
                     TSITHAGLPWELGLAEAHQTLVLNNLRSRIVVETDGQLKTGRDVVIAALLGA"
BASE COUNT         3613 a         8157 c         8293 g         3458 t
ORIGIN      
        1 acccgaaagg cgtgctctac agccaccgct cgacggtgct ccacgcctac ggcggctgca
       61 tggccgactc gctcggtctc tccggtcgcg actgcgtgct cgccgtcgtg ccgatgttcc
      121 acgccaacgc ctgggggctc ccgtacaacg cgccgatgac gggcgccaag ctcgtgttcc
      181 ccggcccgcg catgggcgac gcgccgacgc tgacgcagct gatgaacgac gaaggcgtca
      241 cgctcgccgc cgcggtgccg accgtgtgga ccatgctgct caaccacctg cagcagaccg
      301 gcgagaaact cacgtcgctc gagcgcaccg tgatcggcgg ctcggcggtg ccgctgcaga
      361 tgatccgcga tttccgcgac cgccacggcg tgacggtgat ccagggctgg ggcatgaccg
      421 agacgagccc gctcggcagc ctcagcacgc tgcggcccga gcagttgcag ctgccgcccg
      481 aggagcaggt gctgctgcgc ggcaaggccg gccacggcat cttcggcatt tcgatgaagg
      541 tggtcgacga gcagggccgc gagttgccct gggatggcca gagcgcgggc gagctcaagg
      601 tgagcggacc gtgggtctgc agcggctact tccggctggc ggactcgccg gcccaccgcg
      661 agcccggctg gttcgcgacc ggcgacgtcg cgatgatcca cccgaacggg ttcctgcaga
      721 tcaccgatcg cgccaaggac gtgatcaagt cgggcggcga gtggatcagc tccgtcgacc
      781 tcgagaactg cgcctgcggc catcccgagg tgctgatggc ggcggccatc ggcgtgcggc
      841 atccgaaatg ggacgaacgg cccgtgctgc tggtcgtgcc gcgccagggc tgcaccccgc
      901 cgagcgagtc gatcctcgag cacctcggcc gccatttcgc gaagtggcaa ttgccggatg
      961 cgatcgtgct ggtcgaggcg ctgccgctca ccgccaccgg caagatcaac aagcggtcgc
     1021 tgcgcgacac gtacgggcag gtgctggtgg caccggagac gcaggcgtcg tgagcggtat
     1081 aggacgatgc ctgcaaacat cgctcgagca ttgaaagtgt gacgcagccc taacgtctgg
     1141 catcccgact tgagggtggg tacgcgcttc gacgactctc gattcgtcgc cccgcggagc
     1201 acccgcaatg ctcaggatgg acccttcgac gttgcgccgg gctgcggagc agctcaagca
     1261 gaccacggaa gagcacgcga gctggcacga agacgtgctg cgcgcgatct tctgcgatga
     1321 gctcgcgtcg gccgacggcg tcctgagcgg caccgcacac cgtgactgtt cgttcgggcg
     1381 ctggttctac gaggacgcgc ccaacgggct gcgcgagcag cccgcgttcg tcgccgtcgg
     1441 caaggagcac cagcggctgc accaggtggc cgcgaagctg ctgcgcgccg ccaagggcgg
     1501 tgcgcccgtc gcgcgcgccg atttcgagga cttcgtagcg accacggcac ggctgcgcgt
     1561 gcagaccgag tacctgcgca gctcgatcga cgccgcgctc ggcaaccgcg atccgctgac
     1621 cggggcgcac ggccgcatcg cgatgctgcc ggacctgcac gaactgcgcg cggcgctcca
     1681 gcacggcggc cgtccctgcg cgctcgtgtt catggacgtc gatcacctga agcacatcaa
     1741 cgacgagcac ggtcacgccg cgggcgacgc ggtgctctgc ggtgtcgtcg cccacgtgca
     1801 ggagcacctg cgcagccagg accgcgtcta ccgctacggc ggcgacgagt tcgtgctcgc
     1861 gctgccgggc gccgacctgg acgtggccta cgccgtcgcc acgcgcatgc gtgacggcat
     1921 cgacgacaag ctgttcgtcg cgggcgcggg cggcgccccg gtgcacgtct cggcatcgtt
     1981 cggcctggcg ctgctcgacg ccgacgagcc ggtgacggaa tcgatcaacc gtgcggacca
     2041 ggcgctgctg ctcgcgaaga cggcgggccg cagccgcgtg atcaagtggg acgcggccgt
     2101 gacgaccagc acccgctggc ggaagatcga cgccgacacc gagcgctgag cgcggcggca
     2161 cctcagcgat ggtgcgccga cgcgaacagc tcggcgaacc ggcggtcttc ggtcagcatg
     2221 tggtcggcga cgacttcgcc gagcaggaac cgggtcagct cctcgcggct cgcgcgaccg
     2281 gcggcggcct cggccttcag ccgcatcgcc ttggcgctca gcatcgcgtg cgagcgccgg
     2341 tggcgggaca gaccgcgata gccgcgctgc ttcaggatca actcttcgtc cgcaaaatgc
     2401 cgggcgatct cgtccatcag ccgctcgagg tccgttgcga accgcgagct gcccttgcgc
     2461 agcgcttcga gcacgtcctc ggcgcgcgcg aacagctcgc gatgctggcg gtcgatcgtt
     2521 ggctcgccac attcgaagcg cgcctgccac tgcaggtgca gccctggcgc cccgacggac
     2581 ccgtcgtcgg cagtgctgcg ccggtcgatg acgacctgac cgccgccgcc ggccttcgcc
     2641 tgcgcgagcg ccgattccac gcggctccac cacgatccgc ccggctcgcc gggaaaacgt
     2701 cgggcgagcc cggcactgac ggtcgtctcg ccgaggcctg accaggagcg gcgaccgaag
     2761 tgctccgccg cgctgcgcgc gagcgtctcg accgcctcga gcggaccgcg ggccagcacg
     2821 gcgcactggc cgggcccggt gcgtaccagc gagcccgagg cctcgggcga tctctcctcg
     2881 agcgccccgt gcaggtggcg ctcgatgacc tcggccgctt cggcgccgtg caggtgcacg
     2941 atcaacgcgt ggcgatcgat ctcgagcgcg agcagcacgt cgcgaggatc ggcgggaggc
     3001 agcgcgtcga gacgcggcag ccagagggcc acggccgccg gcagcaccag cgccggcccg
     3061 gtgtcggaca gcaggccgcc ggccgtcgcg gccatccgtg tcgcgccgtg gtctcggctg
     3121 gggtcgtcgg tcatgtgggg tccggagtcc ttccggcggg cacgcgggcg gtcaccgcaa
     3181 ggaacgggcg agactaccga agcccgtccc cgccgcgcga ttgcggatag tgcggatacg
     3241 cctacgtcag gcctgcatgc gcctcagcag ggcttcggac tcggcgagcg ccatgacctt
     3301 cggcaccgga tagtcgcgcg acgcctcgac catcgcggcg cgggcgatct cgggcacgtc
     3361 gtcgggcctg agcgccgcca tcttttcggg aatgccaacc gcccggttca gtgcccgcac
     3421 acgtgagacg aagcgctgcg ccagcacctc ggggggctcg ccggccacgc cggccccgat
     3481 cgcgaccgcc agctcggcaa gccggtcgcg cgtgcgcggc tcgtcgacga ggaagtcgag
     3541 cacgtacggc agcgtgatcg cgttggcgag cccgtgcggc acgccataga gcccgccgat
     3601 cttgtgcgaa aacgcgtgca cgtagcccac gtacgccttg gtgaaggcga gtcccgcgta
     3661 gaacgaggcg agcgccatcg cctcgcgagc ggcgaggtcg ttgccgtcgt cataggcgcg
     3721 ctgcaggttg ccgaaaatca ggcgcacggc cgccaggcag tgctgcgcgg tctcggcgtg
     3781 cggccagcgg ttcacgtacg cctcgacggc gtgcgtcagc gcatccatgc cggtcgccgc
     3841 ggtgatcggc ttcggcatgc cgcgcatcag ttccgggtcg agcgccgcgc ctagcggcac
     3901 gagtttcggg tcgatcacgg ccgacttgac gtgcgcgacc gggtcggtga ccacggccgc
     3961 gaccgtgact tccgaccccg tccccgcggt cgtcgggatc gcgtacagcg gcagcatcgg
     4021 cttcgagact ttgagcatgc cgatcagccg ggccggcgac ttgccggtga ccaccattgc
     4081 gtcgatcact ttcgcggcgt cgatggcgga tccgccgccg accgccagga tcgcgtcgct
     4141 gccgtgcgcc ttgacggcct cgtagccggc ctgcagcacg ggatacgtcg ggtcgggcgt
     4201 gatgccgtca tgcacggcga cgttaatgcc ctgcgcttcg agcgcccgct tgagcggctc
     4261 gaccaggccg agcttcacca gcacggcgtc ggtcacgatc atcacgcgtc gcaggccgaa
     4321 ctggccgagg ctcgtgcaca ggcgttgcgc cgcgccgggc ccgacgaaca gcgtcggacg
     4381 cgggatcgcg accaggcgcg agaccggcca gaggaatggc ttcagcaggc gataggccag
     4441 cagccgcaac tggaacagca tgggcgtgtc caccggattg atgcggtgca tcatgcgacc
     4501 gcggacgcgg agctgccaag tcgcagtgtc cgcagcgggc ggcggcaggg tcaggggcgc
     4561 ggtcacgggc gcgctgtcgg ccgaaccggc cgcgagggcg gcgcgacttg cgcgcagcgc
     4621 gcgctagcgt ggcgcgagcc agccgtggcg cgtgagtgcc tgctggtagt cgatcgcgcg
     4681 acgctcggcg tagaagtcga tcgtgaagcc tagcgccgcg agcagcagca gcccgacggc
     4741 cacgccgtga tgcgaagggc gcccgaacgc gaacaccagc accagagcga gcagcgtcgc
     4801 gacgccgtag gcgtagcgat accacccgaa gctgcggacc acctgcgcga tgcgcggccc
     4861 ctcttccgcg ctgaactgcc ccggatccgc ggcgtgcagc tggacgagtc gtcgcgcctg
     4921 gtcgtcgctg cggaagtaca cggtgccgcc aacgccaaga ccgagcgcgg cgaccacgac
     4981 gagcgcgagc gccagcccgc gcgcgaacgg ctcacggacc caccaccaga gcgtgacggc
     5041 cgccgcgccc gccgcgaggc cggccagcag gatccagaaa gcctcggcct tctcgcccgc
     5101 gaaatacgcc tggaccacct gctctgcact catcgggggg ttgtccgtct caagggatcg
     5161 cgtgcggggc gatgcggtgc gcccacggca cggcggtttc gagctgcgcc gccagccgga
     5221 acagcgtggc ctcgtcgccg atgcgtgacg ccgtgaacag cagtcccatc ggcagcccgt
     5281 cgtcggtcca gtgcagcggc aacgacatcg aagcctgtcc cgtgacgttg gccaccatcg
     5341 tgaacggggc ggcgtcgaac acgggcgcga aggcctcgag caccttggtg cgctggctct
     5401 tgagcgctgc gcccggcagt gcgaccagcg cgcgcagtgc cgcccgtcgc gccgccggca
     5461 actggtacgc gccgatcggc agcgggggca cgccgacgga gggcgtgagc agcacgtcga
     5521 aggcatcggc ccacgcgagc cactggcgcg cgaaggcctg catcgccagc aacgcggccg
     5581 tcacctccgc acccgcctgt gcctcgccca gtttcgcgag cacccgcgtg gtgagctcga
     5641 ggtcgtcgct gcgcggtttc cggccgacca gtgcgggcgc cagccgcaac gttgccgcga
     5701 cctcggccgc gatcagcgac gcgtattgcg cgacgaagac ctcgcgtggc agcggcaggt
     5761 gcacctcgac gagctcgtgg ccgagcgcgg tcagctgccc gaccgcgctt tcgagcgcac
     5821 ggcgattctc ggggtgcatg gcgtggccga gcccggggtc gaagctgtac gcgatgcgca
     5881 accgccccgc cggtgcctgc gtctcgtcga ggtacgggcg cgtcggcggc ggcagcttca
     5941 tcagctgctg cggatagtcg ccgcaggtcg cgtcgagcag cgcggcgcaa tcgcgcacgc
     6001 tgcgcgtgac gacgtgctcg ccgatgaagc cgaaccagac gtccggcagc tgcgggccgg
     6061 tcgggttgcg gccgcgactc ggcttcagtc cgacgaggcc gcagttggac gccggcgtgc
     6121 ggatcgagcc gccgccgtcg ttggcgttcg cggccggcac gatccgcgcg gcgaccgccg
     6181 cggccgaacc accgctcgag ccgcccgggc tgcggtcgag gttccacgga ttgcgggttg
     6241 gcccgaacag ttcgggctcg gtgacgttcg agacaccgaa ctcgggggta ttggtcttgc
     6301 cgaagatgac gacccctgcg gccctgtagc gccgcgtcag ttcctcgtcg gccggcggca
     6361 cgaaatcgcg gaacagccgc gagccgctgc gcatcggctc accggcatac gccgtcatca
     6421 ggtccttgat caggaacggc acgccgctga acggcccgtc ggggaggccg gcggcgatcg
     6481 cggcgcgtgc gttgcgctcc atcaggtgga tgacggcgtt gatgcgcggg ttcacggccg
     6541 cagtgcgcga cagagcctcg tcgagcagtt cggacgcgct cacctcgccg gcacggacga
     6601 gtgccgcgag cccgagggcg tcgtgagcac cgtattcggc aaatgccaag ctcgcctccc
     6661 gctgcgcggc gccgccggtg tgcatgatcg cgcgtccggc cgcgtttggc gacgtcggag
     6721 tgcggccgcg tgcggcccga acgcggcgta ttcaagtaat cgagcgagcg agcgtgccaa
     6781 cgcacgcgtc ggcttgcggg tcgccggggc gggcgcgaca ctgcgcaccc cggatagacg
     6841 gaagcaagaa cgctctctcc atggcacgca cgtactgccg gcgcccgctc acgtccctcg
     6901 tcttcgcgtt ggccacggtg ctcacggggc cgtcgctcgc cggcgcggct cccgtcacgg
     6961 ccgaacgcat cctcgccgcg gaccgggagc cgcagaactg gctcgcgcac gggcgcacct
     7021 acggcgaaca gcgcttcagc ccgctcacgc aggtgaacga ccgcaacgtg gcgcagctcg
     7081 ggcttgcgtg gtcctatgcg accggcacca accgcggcct gcaggcgacg ccgatcgtgg
     7141 tcgacggacg cctgtacgcg acgggcgtct ggagcgtcgt gtacgcgctc gacgcaaaga
     7201 cgggccagga gctctggcgc tacgacccgc aggtgccgcg cgcgtggggc cggtatgcct
     7261 gctgcgacgc cgtcaaccgc ggcgtcgcgc tctggggcga ctcggtgttc gtcggcaccc
     7321 tcgacggtcg actgctggcg ctcgacgcga agaccggcaa gaaacgctgg gaagtgctca
     7381 cgatcgatcc caccaaacca tacacgatca cgggcgcgcc gcgcgtcgtc aaaggcaagg
     7441 tgctgatcgg caacggcggt ggcgagtacg gcgtgcgcgg ctatttttcg gcgtacgacg
     7501 cgaagaccgg caagctggcg tggcgcttct acacggtgcc cggcaatccc agggacggct
     7561 tcgagacgcc cgagctcgcc cgggccgcca agacctggac gggcgaatgg tgggtcggtg
     7621 gcggcggcgg cacggtctgg gactcgatgg cgtacgacgc ggagctcgac acgctgtacg
     7681 tcggcaccgg caacggctcg ccgtgggcgc gctcgatccg ctcacccggc ggcggcgaca
     7741 acctgttcct gtcgtcgatc cttgcgatcg atcccgacga cggcgcgctc aagtggcact
     7801 accaggtcac gccggccgac aactgggact acaccgcgac tcagcacatc gtgctcgcgg
     7861 acctcacctt cgcaggcaag ccgcgcaagg tgctgatgca ggcgccgaag aacggcttct
     7921 tctacgtgat cgaccgcgtc acgggcgagc tgctcgcggc ggacaaatac attccggcga
     7981 cctgggccag tcacgtggac ctggcgaccg gccggccggt ggagacgacg gaggcggact
     8041 ggagccgcac gacgcgcctg atcatcccgg cggctttcgg cggccacaac tggcacccga
     8101 tggcgtacaa cccgggcacc gggctcgtct acatcccggc catgcagccg atgggcatct
     8161 acacgccgtc gcgcgagtac ctcgagaccg gcaagtacac gcgtcgcgag atgttctgga
     8221 accccggcgt cgactggaac gagtacacga agtcgatcta cgggctgatg cagcaattcg
     8281 gtggggcgtt gccgcccgac cgcggctacc tgaaggcctg ggaccccgtc gcgcggcgca
     8341 cgcgctggga gatcgagcat cccgcgttct ggaacggcgg cgtgctgacg acggcgggca
     8401 acctcgtgtt ccagggcacc ggcgacggtc gtttcgtcgc gtacgccgcc gacacggggc
     8461 gcaccctctg gacgatgacg acgcaggtcg gcatcgtcgc gccgccggtg acctacgccg
     8521 tggacggcga gcagtacgtc gcggtgctgg tcggctacgg cggcgccggt gcggtcaccg
     8581 ggggcgaccc ccggacgatg gcgtcgggca agtacgtgaa cgacggccac gtgctcgcgt
     8641 tccggctcgg aggcacggca ccgatgccgc gggtcgccga gcgtgaccag gacattcccg
     8701 agccgcccgc aatcgaggcg acgcccgagc agatcgagcg cggcaagtac gcctacaccg
     8761 ccaactgcat ggtctgccac ggcgcgctgg ccgtctcggg cggcgtcacg ccggacctac
     8821 ggcggctgtc ggcggagaag cactcgatct tcaagcagat cgtctacgac ggcgtgatcc
     8881 acacggccgg catgccgcgg atgtccgacc tggtcagcga gcaggacgtg cgggatatcc
     8941 aggcctatat cgtgcagcgc gcccgggagg accgcgccgc cggccagaag gcggcaccgg
     9001 cccgcaagcc ctgaggaaaa tggggacact ccgaatttcg accagaaatt gacgggttcg
     9061 cgcagtttct tccgaaattc ggagtgtccc cattttcggt gtcgattccc ggaagcgcca
     9121 ttcgactacg gtcatgcgac cagcctgggc aaggaggaag ccatgtcgac agccccgaat
     9181 cccgtgccac ccggcatgca ctcggtcacg ccgcacctcg tctgtgacgg cgccgcgaag
     9241 gccatcgagt tctacgaaaa ggccttcggc gccacgtcgt cgggggccat gaaagggccc
     9301 gacggccggg tcatgcacgc ccagctccgc atcggcgact cgcacgtgat gctggtcgac
     9361 gagtatcccg agcagggcat gctggggccg cgcgcgctga agggcacccc ggtgacaatg
     9421 cacctttatg tcgacgacgc cgaccgctgg tttgaacgcg cgatagcaca aggcgcgaag
     9481 accgtcatgc cgctggccga catgttctgg ggcgaccgtt atggcctgct cgaggatccg
     9541 ttcggccacc gctggtcgat cgcgacgcac aagcgcgacg tgccgcccga ggagatgcag
     9601 caggccgtca agaatctcga cccgacgtgc ggcaagtagc ggcacggacc acgacaaccc
     9661 caacaggagg aaacgacatg ggtatctacg tggatggctt cgtattgccg gtgccgacga
     9721 agcggctcgc cgagtatcgc aagatggcgc gcctggccgg caagatctac aaggagctcg
     9781 gcgtgctcgc ctacgtcgag gccgtggccg acgacgtgac gccgggcaag tggacctcct
     9841 tcccccgcag cgtcaagctg aagccgggcg agacggtcgt gttctcgtac atcgtccaca
     9901 ggtcgcgcaa ggaacgcgat cgcgtcaaca agaagttcat gagcgacccg cggctcaaga
     9961 aatacatgga tcccaaggcc atgccgttcg acggcaagcg gatgttctgg ggcgggttca
    10021 agtcgctggt cgagctgtag gcgcccttgc gcgcgcgagc gctcgcgcgc tgcgctcact
    10081 cggaaggcgt gacccagcga aaggtcaggt tgacccgctc gcccaccgtg cgcgcggttt
    10141 tcggcagcgc gtgcacgtag tggtgctgcg tctcgcccgc catcgacagc aggctgccgt
    10201 ggacgaggtc gatatcgatt ccctgccggc tgcgacggtg gcgcagccgg aaccggcgcg
    10261 tcgcgccgag tgacaacgag gcgatggccg ggtcgcggcc gagctcgggc tcgtcgtcgg
    10321 cgtgccaccc catgccgtcg cggccgtcgc gatagagatt gagcagcacg ctgttgtacc
    10381 ggtgaccggt cagcgcctcg gcgacctcgc gaagctcgcg caattcgtcg gtccaggcgt
    10441 gcggctcgtg cgcggtgccg gagtatgtgt agtgcgcgtc ggggtcgccg tgccaggcga
    10501 cgaggcgcgg cacgagccga ggcgttccga aaatgacgac gtgttcctgc tgccagtcga
    10561 tgccagctcg cagcgacgcg agcaggacgt cggcccgcgc tgcgcccagc gcgtgcggcc
    10621 agtagcgaac gtccgcgtcg ggcaggtcaa gccgccggca cggcggcgtc gggggttccg
    10681 gagtcgtctg catcggctcg gagcgtagct ttcgtgcgct ccaggtgcac gcgcacgatg
    10741 ccgcggggct gcgactggca cgcgaggatc acgcgctccg ccagctgctg cttggtgagc
    10801 acgtagctgg agtccgtcaa cgcacgcacc tggccttcga gcagccggca gcggcaggtc
    10861 gtgcaactgc ccacccggca gcgcgacggc cagggcagtc cgtgcgcgag cgcggcctgc
    10921 agcagcgtct ggccgggctc gacgcggaat tcccagccga acggctcgac gtgaacggtc
    10981 gcggcctcgg gccggcgccg gagcagctcg cccagcctca cgcggccgcc tgcgtggcct
    11041 gcagcgccac gaggccgctc gcgcgattgg ccgccgcggc gagcacccgc tcggtggcgt
    11101 tggcgtaacg gcggtcccag tcgagcacga gcggcgtcat gcgatcgcga taccagccgg
    11161 gcgcgacgta gacgagcagc atgcaggtca ggtacccgta tggcagcgtc ggggcgtccc
    11221 tgtatgagcg cagccgccag aacggcgcgt cgccttcggc gtggtgatgc gaatggcgcg
    11281 cgaggttgaa cagcacgacg ttgctgagcg ccttgttgga gttccacgag tgtcgcggtt
    11341 cgatcggcga cgtcggttcg cgcacgagtc cgtagtgctc gaagtagttg acggcctcga
    11401 gcaccgtcct cccagcgagc ccggccagca cgaacaccac gacgcccgcc gcacccgcgg
    11461 ccaatccgaa agcggccacg atcaatcccg acatgcccca gccgcggaga tagcggttgt
    11521 gccagctcca ggtcggcagg tcgagtttcg ccaggcgatc gcgctcgagc cggaacgcgc
    11581 tgcgcgtctg gccggcgatc gaacggacga ggaacgcgta gaagtgctcg ccacggcgcg
    11641 ccgtcgccgg gtcggccggc gtcgcgaggc ggcggtgatg gccgtagacg tgctcgatcg
    11701 cgaactccgc gtcgaacgtc agcgcgagca gccagcgacc cgcgatcatc gcgccgcgat
    11761 cggccgtgcg gtgcgtgagc tcgtggccgc cgttggtgcc gacggccgcg aacgtgagcg
    11821 ctgcggacag gatgccgccc agccagtgca gcggcccggt cgccgcgcgt gcggcgaaca
    11881 ggtcgtaccc ggcctgcgcc tgcacccacg cgccgaggcc gaacaggtcg cccgagccgg
    11941 ccatccatgc catgacgcag cagagcaggc ccagcgtcgg caacgcgagg tacagcagcg
    12001 cgtcgagcag gcgcggttgc gcgtaggccg gctccgcgta gtcgtcgccg atccactcgt
    12061 cgcccgcaat catgacggcg tagatcacgc cgatgccgag ccacatccag gcgccgccga
    12121 gcgcgatgcc cagcgtcgtc gcgagcacga atccgtgcac ggccaggaac ttcaggtatc
    12181 tgcccacggg tgctcctgga tagaatttag aacaattgtt ctagcgagga tgccgtgccg
    12241 gcgccggggt gtcaaatgcg acccgcggct gcctatactc tggtccgcgg tccgttccgc
    12301 acatgggttt cagcacgatg ccgcgcaggc gtcccaccga gccgaccgac acccgcgagc
    12361 gcatcgccga cgcggccctg tcgttgttca acgccgacgg cacgccggcc gtgtcgacgc
    12421 gtcacgtcgc cgcgcgcctg ggcatcagtc ccggcaacct gtactaccac ttcggcaaca
    12481 aggaagagat cgtcctgcac ctctacgagc gcatcgagac cgaactgcag gcaatcctgg
    12541 cgccgccggc acacgacgcg cgcacgttcg aggaggtgct cggctacctg gacgcgatct
    12601 tcgcccacct gtggaagttc cggttcttct atcgcgacct gaactcgctg ctgcgcgacg
    12661 tgcccggcct caaggagcgc taccgccggc tcgccgaacg cgtgctggcc cgatcgaagg
    12721 cgatcttcac ggagatggtg cagcacggct ggatggaggc ctcggacgag cacctcgacc
    12781 tgctggtcac caacgcctgg atcgtgctga cgcagtggtt cacgcatcgg caggtcgtcg
    12841 aacgccgccg cacgatccag tcatcggacg ttcgtgaggg catccgtcac ctcgtcgcgc
    12901 ttttcagccc gctgctgcgg cggccacagc ggcgccagat cgaggagctg ctcgcgcagg
    12961 acgcgtgacc cgcccgccgg ctaggtaacg cgcctgatgc agccagtccc ccggccgagg
    13021 acaatccgag caccccgccc gaggagcgac tcatgttgac gccgagcatc cgcgcccgcc
    13081 tgaccaatgc cctgctgcgc ctgacggtga agcgcttgtg gcggccggac atcgccatcg
    13141 acgaggtgcg caggcacgcg gctcgctcgg acgtgcgcct gtccggtcgt ccgccgcgtt
    13201 gtccggtcga ggccgtcgag atcgccggcc tgcgcgcgca ctggttcggc gcccccgaac
    13261 tcgccgcgcg tcacggcacc ctcctctacc tgcacggcgg tgcctggtgc atgcacctgc
    13321 cgcggctcta cgccgcgttc gctgcggccc tgtcgaacgc gaccgggttg cgcgtgctgc
    13381 tcggtgaata ccggttggcc cccgagcacc cgtttccggc cggcatggac gactgcctgg
    13441 cgacctatcg cgcgctcgtc gacgggccgc atggcacccc ggtcgcgatc gcgggcgact
    13501 cggcaggcgg cagcctgtcg ttgatcacgc tgatgcgcgc gcgtgacgca gggttgccgc
    13561 tgccggcctg cgccgtgctg ttgtcaccgt cgacggacct cacgtttggc gggccgtcga
    13621 tgcgctacaa cgccctggcg gatccgatgt tcagcgaagc gtcgatggac ctgttgccgg
    13681 acatctattg cccggggcag gaccgcggaa atccgctgct gtcaccgctg ttcggcgact
    13741 ggtccgggct gcccccgctc tacttcctgg cggggtccac cgagatgttg ctcgacgatt
    13801 cggtccgcgc ccacgatcgc gcactgcagg cgggcacctc gtcgcgcatc gacgtctggt
    13861 atcagttgcc gcacgtgttt ccggtgatgc acaggctgcc cgagtcgcgg gtggccctgc
    13921 gggcgatcgc ggcgttcatc gatcagcatc gcgcactgcc gcgcgggctc gtgcctcccg
    13981 tcgtggatcc gctcgtagcg gctgttgccc aggtgggcat ggcctcagtc gaggcttcgc
    14041 cgggcggccg ttcctcgcct tgatgatcaa cgcctcaccg gctccgcgtg cagacgagcg
    14101 agcacgggtc gctccgcgcg tcgggcctcc gcgcggcgcc atgcccgctc gtggaaatgg
    14161 aacgcgaccg tgttgacgat cggttcgatc agtccgacgc ccgtcgccac ggcgattgaa
    14221 ccggtgagca ggtaggcgac gctgaacgcg acgccgaagt gcagcagtgc gaacgtgaat
    14281 gtcttcagga gtgcctggtt catggatcgt cggccatggt tgaaacgcgg aagcggacga
    14341 tgcctcgcca cgcgtcgacg atccaatcga ttgtcggaat cattctgata ggcgcgccct
    14401 accgctgccg gcacgtacgg acgctgcgta tttacgctgc ggcggccggc ggctcaccat
    14461 ggcctgccgg cgcgagacaa ggccggcaag agaggcttcc gtgcagggcg agccgctcgc
    14521 ggcgcccgtc cccgatcggc acgtcgcgca gacgcccgcg ctcacgctgc gcgcccgcgg
    14581 cctgaccaag acctacggcg ccggcgaact tgcggtgcat gcgctgcgcg ggatcgacct
    14641 cgagctcgcc acgggtgagc ttgccgtgct gctcggcccg tcgggcagcg gcaagtcgac
    14701 gctgctcaac atcctcggcg ggctcgacac cgcgacgtcc ggcgaggcct ggttcctcga
    14761 ccacgacctg acgcgcgcga gcgaccgcga gctcacgcgt taccggcgcg agcacgtcgg
    14821 cttcgtgttc cagttctaca acctgatccc gagcctcacg gcgctcgaga acgtcgcgct
    14881 cgtgaccgag atcgcgaccg caccgatgga ccccgcggtc gcgctccgac tggtcggcct
    14941 cggcgaccga gtgcaccact ttcccgcgga gctgtccggc ggcgagcagc agcgtgtcgc
    15001 gatcgcgcgc gcgatcgcga agcagcccgc gatcctgctc tgtgacgaac cgacgggtgc
    15061 gctcgacagc aagaccggcg tcctcgtgct gcaggcggtc gagtccatcc accgcgagct
    15121 cggcacgacg accgtgttga tcacgcacaa cgcggtgatc gcggagatgg ccgaccgggt
    15181 catcgtgctc ggcgacggca tgatccagca ggcgcgccgc aacccgcggc gccgcgctgc
    15241 cagcgagctg gcctggtgat gcgggcgctc gatcgcaagc tgtcgcgcga cctctggcgc
    15301 ctgcgcgggc aggtgctcgc gatcggcatg gtgatcgcgt cgggcgtggc gttgctcgtg
    15361 atgtcgttga cctcgatcga ggcgctcacc gagacggcga ccgcctacta cgagcgctac
    15421 cggttcgcgt ccgtgttcgc tcgcgtcgaa cgggcgccgg agcacgtcgc cgcgcggctg
    15481 gcggccatcg agggcgtgca gtccgtcacg acgcgcgtcg tacgcagcgc cgtgctcgaa
    15541 gtcgaggatt tcgccgagcc cgtcatcggc caactgatat ccgtcccgga gcgcggcgag
    15601 cccccgctga acgcgctggc gatgcgccag ggccgttacc cgagggaaac cgcgcccgac
    15661 gaggtcgtgc tctcggagcc gttcgcgcag gcgcaccatc tcgcgctcgg cgatcacgtc
    15721 caggccgtgc tgaacggccg ctggcgccgg ctgaccgtcg tgggcgtggc actctccccg
    15781 gagtacgtct acacgatcgg cccgggcgcg ctgatgcccg acgaccgccg cttcggcgtg
    15841 ctctggatgg gggaacgcgc gttgcaggcc gcgttcgacc tcgaaggcgc gttcaacgac
    15901 gtctcgctgg gactgcttcg cggcgccgac ccggacgccg tggtcgaccg ggtcgaccga
    15961 atgctcgcgc gctacggcag catcggcgcc tactcgcgcg aggaccagct gtcgaactgg
    16021 ttcctgatga acgagatcgc gcaactgcgc acgatggcgc ggctactgcc gacggtcttc
    16081 ctggcggtgg cggccttcct gaccaacatg gtcctggcgc ggctcgtggc cgtggagcgc
    16141 agcgagatcg gactgctcaa ggccttcggg tactccggcg gcgaaatcgg gtggcactac
    16201 gtcaagctgg tcctcgtgat cggcgcggtc ggcatcgcgc tgggctggtt cctcggctac
    16261 tggctcgggc tgtacaacac cgtcacctac gccgagttct acaagttccc gttcctgctg
    16321 taccggccgg gccccggacc gttcgcgatc gccgcgctcg cgagcctcgc ggcggcgctg
    16381 ctcggcacga tccgcgccgt gcgtgacgcc gcctcgctgc cgcccgcgca ggcgatgcag
    16441 ccgccattgc cgacccggtt ccaccacggc ctgctcgccc gctggtcggc gctgcgatcg
    16501 ctggaccagc cctcccggat cgtgctgcgc caggtggccc gctggccggt gcgctcgttc
    16561 gtggcgagca tcggcatcgg gctggcgatc gcggtgctga tcggctcgat gcaatggctc
    16621 gacgcgatcg atcacatcga ggacgtctac ttcctgcagg cccagggcca ggacgtgacg
    16681 gtcggcttct cgcaggccca gccggccgac gcggcgcgta cgctcggccg cctgccgggc
    16741 gtcatggcca cggagccggt gcgtgcggtt gcggcgcggc tgtgcgccgg cccgcgcatg
    16801 cagcgcgagg ccgtgcaggg cgtgcccgcg cacccgcagc tgagccgcgt ctacgatgct
    16861 tcgggtcgcg ccgtggagct gccgcgcgag gggctcgtga tctcgaccat gctcgcacag
    16921 ctgctgaagg tggggcgcgg cgacacgatc gtcgtggaag tgctggaggg tcgccgcccc
    16981 gtgttcgagg cgagggtcgc cgaggtcttc gaaacgtaca tcggcagccc cgcctacatg
    17041 gagatcggcg cgctcgctcg cctgctgcag gaaccgccga gcgtgaccgg cgtgcacctg
    17101 cgcatcgatc cggtcgagca ggcccggttc tttcggacgc tcaaggacgt gccgcgggtc
    17161 agcgccgtga cgctgcgcga ggccgccatc gcgacgtacc gggagacgat ggcgcgcacg
    17221 ctgctgatct tcgtctcgtt cttcgtggtg ttctcgtgcg cgcttgcgtt cggggtcacg
    17281 tacaacaccg cacgcatcgc gctctccgag cgtggccggg aactcgcgac gctgcgcgtg
    17341 ctcgggttca cgcgtgcgga gatctcgtac atcctggtcg gcgagacggg cgtactcacg
    17401 ctcgccgccc tgcccctcgg gctcgcctgc ggttatgcgc tcgcacgcgc gctcgtggcc
    17461 ggcctccaga cggagctgtt ccgcgtgccg ttcgtgatca gcgcggcgac gtacggctgg
    17521 gcgctgctga tcggcatcgc tgccaccgtc gcctcggtgc tgctcgtgcg ccgccgactc
    17581 gaccggctcg acctggtggc ggtgctcaag acgcgggaat gaaggagctg ttcatgcagg
    17641 ccgccttgcg caagcgcctc gtgttgtgga tcccgcccct ggcgctcgtg ctgatcgcgc
    17701 tggcgtggct gctgcggccg cctgccgcgg ccgtcgacct cgccgaggcg cggcacggac
    17761 cgttgcgggt ctccgtgagc gacgacggcg aaacgcgggt ccgggacgtc ttcgtggtct
    17821 caaccccggt gaccggcctg atgcgtcgga tcgaactcga ggccggcgac gcggtcgttg
    17881 cgcaggagac cgtcgtcgcg cggatcgaac cctcggaccc tgccttcctc gaccgccgca
    17941 gcgagggcga ggccgtggcc gcattgcgag ccgccgaggc cgcacgtacg cacgccgagg
    18001 ccgaggtccg gagagtcgag gccgagctcg agttcgcgca gtccgaactg cgacgttacg
    18061 aggggctcgt gcggcgcgcg gccatctcgg agaacgacct cgatgcggca cgccgtcggg
    18121 cccgcacggc ggaggccgcg ctgcaggagg cgcgtgccgg cctccgggtc cgggcgtcgg
    18181 aagtcgagca ggcgcgtgcg cggctgcggg ccccgggcgc cccgcgggac ggggactgtg
    18241 cgtgcgtggt cgtgcgttcg ccggtgagcg gccaggtcct gcgggtgctg atcgaaagcg
    18301 agggcgtcgt cgccgttgcc gcacccctga tcgaagtcgg tgacccccag caactggaga
    18361 tcgtcgtcga cctgctgtcg acggaggcgg tgcgggtggc gccggggcag aaggtcctcg
    18421 tggaaggctg gggcggtccg cagcccctcg aggccgtggt gcgccgcgtc gagccgttcg
    18481 ggttcaccaa ggtctcggcg ctcggcgtcg aggagcagcg cgtcaacgtc gtgatcgatt
    18541 tcaccgggga gcccgcgcag tggtcgcgcc tcgggcacgg cttccgggtc gagccgcgca
    18601 tcgtgctctg ggaggccagc gacgtgctgc aggtgccgtt gtcggcgctg tacagggaag
    18661 gctctacgtg gcgtgtcttc cgcgaggtgg atggccgcgc ccgggccaca gacgtgcgca
    18721 tcgggcacga cgacggcctg gaggtcgagg tcctcgacgg gttgcagccc ggcgaacgcg
    18781 tcgtggtgca cccgagcgac cgggtcgagg aggggacgag gatcgtccag cggcagttgc
    18841 gctgattcgg ctggtcagcg ctgcgcgacg ctgcgtggtt ccgctggatc gccgacgacc
    18901 tcgatccggg cgcggacgac cccacgccgg agcatgccga tctgcgcggc agcggcccgt
    18961 gacaggtcgg cgatgcgctg gccgacgtgc ggcccgcggt cgttgacgcg gaccaccacg
    19021 gagcggccgt tgcgcaggtt ggtcaccttg accgccgtcc cgaacggcag cgtcgggtgg
    19081 gccatcgtgt agccgtcgga atcgaagcgc tcgccgctgg cggtcggccg gtcgtgaaac
    19141 gccgccccgt accaggagac gaggccctcc tggacggcgg agaatccgcg cagcacggtc
    19201 ctgtcgagtg ccaggccgcc ggccgcctcc ggcgccgccg gaagcgcggc caacgcgagc
    19261 gccgcgccga ggagcgcgca caccggccct cgagcgcgaa tcgcgcgccg cggatcgggg
    19321 cgccgcggca gccgcgactg tgggaggaag gaaggcatgt gggcgggcag cgtaagacac
    19381 caggccgccc gagtcgcgga cccggtcggc actttgggcg gtcagttgcc ctttcgctgg
    19441 ccgatgacct tcttcatgtc ttcgaactgg cgctggttcg aattccgcgc ctcgtacatc
    19501 tccgcctcgg tgaggcaggt gcgctccttc tggacgtggg agccgagctt gcgcgtctcg
    19561 cggcaataca tggtcttgcc gtcttcattg acgatcttgt accccagctt ctgggcctct
    19621 tcgagcgtcg tggcctcggg tacgtcggtg gccgcgtagg tcgtggccgg cggcggtgcc
    19681 gcagtgttct gcgatgcaca accggcgaga acgccgacgg ccgcgaagat cagcagtgaa
    19741 cgcatttcag gactccttgc gtgcgcgaaa tgcacggcca gggatcacac ccggaacgca
    19801 acggccgccc acgcatggcg ggcgcccgcg cagcatacgc gagcgggctg gctacgtgca
    19861 tcagcgggat aaagccgcgg cgcttcaaca agttagaacg cgccagacga tgctgtggcg
    19921 cactcgcccc gtgaaatggt tatcactcca gacggctttg ttgcactgca ttgacgcagc
    19981 cggcgctaca tgtcatatga cgtgcgtatc cacgatcgtg cctggtcagg gtgcggctgc
    20041 gcgggcgctt gcgaccgcgg caggcgtcgc cctgcgggcg ccgctgcacg aggccggacg
    20101 cgatgacgca taccccccac gaccacggcg acctcgcgac cggaactcgc gccgggctcg
    20161 gcgacggccc ggcgcagctt gggccgccgt cgaagcaggg tctctacgac ccgcgccacg
    20221 aacacgacgc gtgcggccag ggtttcgtcg tggacatcaa gggccgcaag tcgcatcgca
    20281 tcgtcgagca ggcgatccag gtgctgaaga acctggacca ccgcggcgcg tgcgggtccg
    20341 aggtcaacac gggcgacggc gccggcatcc tcatccagat gccgcacgcc ttcaacgagg
    20401 aagtctgccg caaggcacgc atccagctgc cgggtcccgg cgagtacggc agcgggatca
    20461 tattcctgcc gcgcaacccg accgtgcggc ggcaggtgga gcagcgcttc gagcaggtcg
    20521 tgcagtcgga aggcctgcac gtgctcggct ggcgcaccgt gccgaccagc aacggcatgc
    20581 tcggtgagac ggcgcgttca tgcgagccgt tcatgcggca ggtgttcatc gggcggccgg
    20641 cggagctcac ggacgacctc gccttcgagc gcaagctcta cgtggtgcgc aagcgggcct
    20701 acaacgacat ccgcacctcg acgatcgcgg gcgccgaata ctggtacgtc gtgagcctct
    20761 cggcccgcac gttcgtctac aaggggatgc tgctcacgac gcagctcgac cagtacttcc
    20821 acgacctgca gcacccgctg ctcgagtcgg cgctcgcgct ggtgcactcg cgcttcagca
    20881 ccaacacgtt cccgagctgg gaccgcgcgc atccgtaccg ctacatcgcc cacaacggcg
    20941 agatcaacac ggtgcgcggc aactccaact ggatgcacgc gcgcgaggcg cggttcgagt
    21001 gtgaggcgtt cggcgacgac atccgcaaga tccgcccgat catcaacccc aacggcagcg
    21061 actcgggcat gttcgacaac acgctcgagc tgctctacct ctccggccgc tcgatgccgc
    21121 acgcggtcat gatgatgatc cccgagccgt ggtcgaacca cgagtcgatg gacgacggca
    21181 agcgggcctt ctaccagtac cactcgagcc tgatggagcc gtgggacgga cccgcctcga
    21241 tctcgttcac cgacggcacg ctgatcggcg cggtgctcga ccgcaacggg ttgcgccccg
    21301 gtcgctacta cgtgaccaac gacgacctcg tgatcctggc ctccgaagcc ggcgtgctcg
    21361 acgtgccgcc cgagacggtg gtgcgcaagg gacggctgca gccgggcagg atgttcctgg
    21421 tcgacacggc gcagggccgc atcatcgagg acgaggagat caagcgcacg ctcgcgaccg
    21481 cgcgccccta ccgcgagtgg ctcgacgagc acctgatcca cgtcgacgac ctgccgcagc
    21541 cgcccgagct gccgccgtcc gacccggact cgctgctgca gcggcagatc gcgttcggct
    21601 acaccttcga ggaccagcgc gtcgtgctga cgccgatggc gcgcgacggc gtcgaggccg
    21661 tgggctcgat gggcaacgac acgccactcg cggtgctgtc ggcccgaccg cgcctgctct
    21721 acgactactt caagcagctg ttcgcgcagg tgaccaaccc gccgatcgac tgcatccgcg
    21781 aggagatcat cacgtcggcc gaaacgcggc tcgggtcgga aggcaacctg ctgaacccgc
    21841 agccgtccga ctgccgccgg ctcgagctca agtggccgct cgtcacgaac gaggagttcg
    21901 cgaggatccg tcgcatggac ctgccgggcc tgcgcgtcgg cgtgctgccg accctgttcc
    21961 gcgtgagccg cggcgaaaag ggcctcgtca agtcgatgga ggagctgcgg ctgatggcgc
    22021 ggcgcctgat cgaggaagaa gaggtcaacg tgatcgtgct gagcgatcgc ggcgtcaaca
    22081 aggacttcgc gccgatcccc gcgctcatgg ccgtggccgg cctgcaccac tacctgatcc
    22141 gcgaggggct gcgcacgcgc gtgagcctgg tgctcgagac cggcgaggcg cgcgaggtgc
    22201 accacttcgc cgtgctgatc ggctacggcg tcagcgcgat caacccttac ctggccttcg
    22261 agaccatcga ccagatgatc aaggaaggcc tgctcacgaa catcgaccac aagaccgcct
    22321 gcaggaattt cgtcaaggcg gccgcgaagg gcgtcatcaa ggtggcgtcg aagatgggca
    22381 tctcgtcgct gcagagctat cgcggcgcgc aggtgttcga ggcggtgggc ctgcggcaag
    22441 acgtcatcga cgaatacttc agctggacgg cgtcgcgggt cggcggcatc ggcaccgacg
    22501 tgatcgcgca ggaagtgctg ctgcggcaca acgcggcgtt ccccgagcga cgcggcctgg
    22561 ctgcgcacac gctgcccgtg gggggccagt accagtggcg cgacgacggc gagcagcacc
    22621 tgttcaaccc cgagagcatc caccggctgc agaaggccgt gcgcacgggc agctacgaga
    22681 cgttcaagtc gtactcggcc ctggtcaacg ggctgtcatc caacctgtgc acgctgcgtg
    22741 gactgctcga gttcaagggc gccgagccgg ttccgctcga ggaagtcgag ccggtcgaga
    22801 gcctgatgaa gcgcttcaag accggcgcga tgagctacgg ctccatcagc aaggaggcgc
    22861 acgagacgct cgcgatcgcg atgaaccgca tcggtggcaa gtccaacacc ggtgagggcg
    22921 gcgaggatcc cgagcgttat cgctggacca acgagcacgg cgactcgaag aactcggcga
    22981 tcaagcaggt ggcctcgggg cgcttcggcg tgacgagcga gtacctcgtc caggcgaggg
    23041 aactgcagat caagatggcg cagggtgcga agcccggcga gggcgggcag ctgcccggct
    23101 ccaaggtcta tccgtcgatc gccaagaccc ggcacacgac cgcgggcgtg ggcctgatct
    23161 ccccaccgcc gcatcacgac atctactcga tcgaggacct ggcggaactg atccacgacc
    23221 tcaagaacgc gaaccgccgc gcgcggatca gcgtcaagct ggtggccgag gtcggcgtcg
    23281 gcacgatcgc ggccggcgtc gccaaggccc acgcggacgt cgtgctgatc agcggctacg
    23341 acggcggcac cggtgcttca ccgcagacgt cgatcacgca cgcggggctg ccgtgggagc
    23401 tcggcctggc cgaggcacac cagaccctgg tgctcaacaa cctgcgcagc cgcatcgtcg
    23461 tggagaccga cgggcagttg aaaaccggcc gggacgtggt catcgccgcg ctgctcggcg
    23521 c
//
LOCUS       DASPFR010000321         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1682495, whole genome shotgun sequence.
ACCESSION   DASPFR010000321 DASPFR010000000
VERSION     DASPFR010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682495"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..689
                     /locus_tag="VD737_08500"
     CDS             45..689
                     /locus_tag="VD737_08500"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HXW10640.1"
                     /translation="MNARLMLRLGLFAIVAFVLGLVAARWLLSARDAPPPVTENATVM
                     QPPRAIPALDLLDEDGQPFSADSLRGRWTVVFFGFTYCPDVCPTTLTTLAQMNKQLTD
                     LPSTARPRVMLFTVDPERDTAERLKAYVQFFDPDFGAATGTLPAVEQAAAAFAIPFAK
                     VPLAEGGYTMDHGAGVFFVGPDGNVHAYSSPPLRADTLARDYRKVVRWFEETRG"
     gene            686..1564
                     /gene="asd"
                     /locus_tag="VD737_08505"
     CDS             686..1564
                     /gene="asd"
                     /locus_tag="VD737_08505"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HXW10641.1"
                     /translation="MTALAERSGGDELFALLQRALPRHWLSKGMHRLARSRQRPIRNA
                     LIGTVLRGYPQIDMNEARQPDPFAYPSFNAFFTRELRPEARPIAEDVGCAVSPVDGTV
                     SQLGRTDAGKLIQAKGMDYTCDALLADTASAARYVGGSFACLYLAPYNYHRIHMPLDA
                     VLRATRYVPGHLFSVNASTARSVPQLFARNERVVCDFDTDHGPLCMVLVGALFVGSIE
                     TVFAGEINPPRGRGGQVRAIEAGVGRKFARGEELGRFNMGSTVIVLFGEPAHFASRVE
                     PDSAVRMGQPLARFGP"
     gene            1561..2568
                     /gene="epmA"
                     /locus_tag="VD737_08510"
     CDS             1561..2568
                     /gene="epmA"
                     /locus_tag="VD737_08510"
                     /inference="COORDINATES: protein motif:HMM:NF006828.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HXW10642.1"
                     /translation="MSDGASRCIVAEVADWRPTASLDVLRLRAGLLARARTYFDATGA
                     LEVETPALVRHPVTDVHLDALRVVDDGSAEATVGFLHTSPEYAMKRLLCAGAPDIYQV
                     CHVFRAAERGRRHNPEFTMIEWYRLGLDHHALMRDVEALIRALLDGWRDAPPSVHVSY
                     RDAFRAAVGLDPLDAAVDELVAALEANDVPLPASMRAAPARDDVLDLAMGTLVADAFD
                     PDRLTFLYDFPASQAALAQVRGEVASRFEAFWGPLELANGFHELGVATEQRARFEADR
                     VERRSQGRADRSIDERFLAALEHGLPPCAGVALGFDRLVMVAAGAASIDDVVAFPCER
                     A"
     gene            2605..3102
                     /locus_tag="VD737_08515"
     CDS             2605..3102
                     /locus_tag="VD737_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXW10643.1"
                     /translation="MHELTDTSDLDKPVLYTELARSLQALLAGESNLVAAAANMSSLL
                     YWSLPDLNWAGFYLVDEPSGDLLVGPFHGKPACVRIPLGQGVCGTAAARRRTVLVEDV
                     HAFPGHIACDSASNSEIVVPVLSGDRLVGVLDLDSPKKGRFDEEDARGLERLVEVFVA
                     ATNSG"
BASE COUNT          470 a         1165 c         1107 g          540 t
ORIGIN      
        1 gggctgggcg tggtcggctc ggtcattgtg gaataatcgc agcgatgaac gcccgtctca
       61 tgctccggct cggcctgttc gccattgtag cgtttgtgct cgggctcgtt gccgcccgct
      121 ggctgctctc ggcccgcgac gcgccgccgc ccgtgaccga gaacgccacg gtgatgcagc
      181 cgccccgagc catcccggca ctcgacctgc tcgacgagga cgggcagccc ttcagcgcgg
      241 actcgctgcg cgggcggtgg accgtcgtgt tcttcggctt cacgtactgc ccggacgtct
      301 gccccacgac gctgaccacg ctcgcgcaga tgaacaagca gctcacggac ctgccgtcga
      361 ccgcgcgccc acgggtgatg ctgttcaccg tcgacccgga gcgcgacacg gcagagcggc
      421 tcaaggcgta cgtgcagttc ttcgacccgg acttcggcgc ggccaccggc acgctgccgg
      481 ccgtggaaca ggccgccgcc gcctttgcga tcccgttcgc gaaggtgccc ttggccgaag
      541 gcggctacac gatggatcac ggcgccggcg tgttcttcgt cggacccgac ggcaacgtcc
      601 acgcgtattc gtcgccgccg ctgcgggccg acacgctggc acgcgactac cgcaaggtcg
      661 tgcgctggtt cgaggagacg cgcggatgac ggcgctcgcc gagcgatccg gcggggacga
      721 gctgttcgcg ctgctgcagc gtgcgctgcc gcggcactgg ctgtcgaagg gcatgcacag
      781 gctcgcgcgc agccggcaac ggccgatccg caacgcgctg atcggcaccg tgctgcgcgg
      841 ctacccgcag atcgacatga acgaggcgcg acagcccgac ccgttcgcct acccgtcgtt
      901 caacgccttt ttcacgcggg agctgcgccc ggaagcgcga ccgatcgccg aggacgtggg
      961 ctgtgcggtc tcgcccgtgg acgggacggt gagccagctc gggcgcaccg acgcgggcaa
     1021 gctcatccag gccaagggca tggactacac ctgcgatgcg ctgctggccg acacggcctc
     1081 cgccgcgcgt tacgtgggcg gcagcttcgc gtgcctgtac ctggcgccgt acaactacca
     1141 ccgcatccac atgccgctcg acgccgtgtt gcgcgcgacg cgctacgtcc cggggcactt
     1201 gttcagcgtc aatgcctcga cggcgcgcag cgtcccgcag ctcttcgcgc gcaacgagcg
     1261 cgtcgtctgc gacttcgaca ctgaccacgg cccgctctgc atggtgctgg tcggcgcctt
     1321 gttcgtcggc agcatcgaga ccgtattcgc cggcgagatc aacccgccgc gcggccgcgg
     1381 tggccaggtg cgcgcgatcg aagccggtgt cggacggaag ttcgcgcgtg gcgaggaact
     1441 cggccgcttc aacatgggct cgacggtgat cgtgttgttc ggcgagcccg cgcacttcgc
     1501 ctctcgcgtc gagccggact ccgcggtccg catgggccag ccgctcgcgc gcttcggccc
     1561 atgagcgacg gcgccagtcg ctgcatcgtg gcagaagtgg cggactggcg gcccacggcc
     1621 tcgctcgacg tgctgcgact gcgtgccggg ctgctcgcac gcgctcgaac ctatttcgac
     1681 gcgaccggcg cactcgaggt ggagactccg gccctggtgc gtcacccggt caccgacgtg
     1741 cacctcgacg cgctgcgcgt ggtggacgac ggctcggccg aggccaccgt cggcttcctg
     1801 cacacctcgc ccgagtacgc gatgaaacgg ctgctctgcg caggcgcgcc ggacatctac
     1861 caggtctgcc acgtgttccg cgccgccgag cgtggccggc gccacaatcc cgagttcacg
     1921 atgatcgagt ggtaccgcct cgggctcgat catcatgcgc tgatgcgcga cgtcgaggcg
     1981 ctgatccgtg cgctgctgga tggctggcgc gacgcgccgc cgagtgtgca cgtcagttat
     2041 cgggacgcct ttcgcgcggc cgtcggcctg gatccgctcg acgcggcggt cgacgaactc
     2101 gtcgccgccc tggaggcaaa cgacgttccg ctgccggcct cgatgcgcgc cgcaccggcg
     2161 cgcgacgacg tactcgacct cgcgatgggc accctcgtgg ccgacgcgtt cgacccggat
     2221 cgcctgacgt tcctgtacga ctttcccgcg agccaggccg cgctcgcgca ggtgcgcggc
     2281 gaagtggcct cgcgcttcga agcgttctgg ggaccgctcg agctcgccaa cggcttccac
     2341 gaactcggcg tcgcgaccga gcagcgcgcc cgcttcgagg ccgatcgtgt cgagcgtcgc
     2401 tcgcagggcc gggccgatcg cagcatcgac gagcggttcc tcgccgcgct cgaacacggt
     2461 ttgccaccct gcgccggagt tgcactgggg ttcgatcgac tggtcatggt cgccgccggc
     2521 gcggcgtcga tcgacgacgt ggtcgcgttc ccgtgcgaac gtgcgtgagc gcgcgaccgt
     2581 taccagacga ggatccatgg ccagatgcac gagctgacag acacgagtga tctcgacaag
     2641 ccggtgctct acaccgagct ggcccgcagc ctgcaggcgc tgctggccgg cgagtcgaac
     2701 ctggttgcgg cggccgccaa catgtcgtcg ctgctctact ggagcctgcc ggacctgaac
     2761 tgggcaggct tctatctcgt ggacgaaccg tccggtgacc tgctggtcgg cccgttccat
     2821 ggcaaacccg cgtgcgtgcg gatcccgctc ggccagggcg tctgcggcac ggccgccgcg
     2881 cgacgccgga ccgtgctcgt cgaagacgtg cacgcgtttc ccggtcacat cgcctgcgac
     2941 tcggcatcga attcggagat cgtcgtgccc gtgctgtcgg gcgatcgcct cgttggagtg
     3001 ctcgatctcg acagcccgaa gaagggtcgc ttcgacgagg aggacgcccg gggactggag
     3061 cgactggtgg aggtgttcgt cgccgccacg aacagtggct agtggttagt ggtcagtggc
     3121 tagtgaggaa aaagagcgtc accgctgcag cagcctttct cttccgtcca ctagccacta
     3181 gccactagcc actagccact ggccactggc cactagccac tatcccttgg cgcggctcaa
     3241 atactccgcc gtccgcgtat ccacgcggat cacttcgcct tc
//
LOCUS       DASPFR010000322         6364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_582657, whole genome shotgun sequence.
ACCESSION   DASPFR010000322 DASPFR010000000
VERSION     DASPFR010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582657"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>6364
                     /locus_tag="VD737_08520"
     CDS             <1..>6364
                     /locus_tag="VD737_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10644.1"
                     /translation="PRVLTGVSVGTGTIPAGNPEGEEEEAPQALPPGFEALEGIRRLI
                     ETGAGAFNLDDIGSAAIDTFDELRDALDALDGDDGNVTFQTVGDTTTFTVEIVKEITG
                     TADFTVEAEEFGGEIDLGGVIDISADVVLTLEFGFDPGGFFVNTGASGEELKLHNIVL
                     EGDARAAGRFGFLDIEAEVEAFTVDDGIELVVDLQDPMGDGKLRVSDFGSSLGDKVEV
                     TLNNPGVDDVTLAVTASAAAILPGLESIDLGGAQLTLTWQDITDPGTLDITAGGGLAQ
                     DLIDFLSVDVQEVLDQVSQLDELADALDGFDVPLLQDTLSALADAADFISEEIIQPLT
                     SLSGGQASFNSIQNLIMRLARSTGVDPAALGLAYDDGELTWHLDFGTGFVASDSLDLG
                     FNSDSTGLADLSFSTDGSIEGTVGFELTVGIDIGAIVGGAPADEWFFLRDASASATLD
                     IEATAINAEARFGFLSIGIEGGTAEAEAGFLITLNDPDNTDGKNRITLSELLDGLSSP
                     GDLVSFDLTGLNAEGDADPATDANLSLTLPLVIPFLDVDLDDDQQLILLWSNLEDPDT
                     ISLALPEGLLENILPDFTNMDAGTLVSLLGTISNWLEDFRRNFDVANIPFVGTALDEA
                     LQFADLFSDTLLFDDHDDEDDATFTEKLLDVDGRPTFDTVQQMVGRLVTILGLPEDQV
                     FYDDADETIYINLEIGDVDSASNFGFVDLPIKFDLLQDLAPIAELSSDSTIRLSAGGG
                     ITLTFGIYLGNAGAVTLTNDTLLADLKGGIAFSQARVVATPNEVTTVHGRLSGNAKFK
                     LSVNGGDEEEIVIDNDDTEDNTSVQHLVDDINDALELAGLDTQVVAEQASDDGNPINR
                     INLRALGGVSTLTLSAQQGDPAITQIGFKASMTAAPDPDDGNQLKIKGQADVSGVVGR
                     LSGNAVMQVSLDTVNGGTPVIVTVDADDTSTNRNILDVVVDVQNAFDDAGFEDEIEVT
                     SLGKHLIFRTLNPAATSLTINVAGGNPAQQLGLATSNLGNSHDLLITTSNGVVHPITF
                     GPEVDTLGEVLSAISSQTGGAVSASFSDNDTRILLTDNLEQTTAVFKVNNAVGSTAAF
                     DLAIFGFDTVEPEPEDDPRDFQIEGGLLGGVDPLDRLFVQNAQAQASVGIVTPQLNED
                     GEIEDTDLDGSTTDGLDAEARFGFVGIHGHGGISNLAGDGPLTASIDVALKPADAEAF
                     DPEAKITLKDLIDNIGNIGDFLDGPTIDGSGKVELAVTITPSFSDIETGAEPTLIIEL
                     TSLAEILDGDEDGDAGYTIDTENFEDLLNFDEIGFDDILAALRALVDFLGQFEQFGFL
                     NDDIPLINISINDMLSFVEDFEEALDEVQDNPAGTVQVLETKLKEAFGIPQSSDVLQL
                     DLVDGSILRIDLNFEPSFSESLPVSLELPIETGPIDLSGNANLRAEGALDLTLAFGID
                     LGFLTGEDDFDIENVLWVFEDTGIAGSLTAGADDISFTAALGGLGARIVDGEAEITGA
                     FNLGLAASAMTAGTGQNRRISFTDLIGDIPGSIDASLTGEVTALLPVYFPTESSFRGN
                     IEVGGALELTPENGLELNGTLVGEDVVLDGFVRIPDEIFSLDFSQFSALDNLLLIIDG
                     VDGFLGLLQDLFSGQVGGFTLPLVGDQLAEAADVIEDFRKDFIDGLRDAVETAADPDQ
                     NYISEQLFALLGPSGLNILLDGDDAGSDVNVGDIVLDTNVDEGGEGEEPPPPEEIFMQ
                     WNMRLGSTLLNAGEGLDFDIGIPGLGLETRGRIEVEIDWELALGFGVGISRGGFYIDT
                     SDLNEFEVEAEVTLPDAGLTGRLLFLQLDADNNGNTGLGATFGVDIFREGGGDPLIGI
                     ADLGSLGIDIGVAAHANIDLGLELKLNSELVPNSISKVFPKIVGDFVLDFEIGDFTDP
                     ENPVLVGFDEIGNAFEEGLKRVAFENVGLDLGSFISDFLGPVVDEVQKFTGPIQPIID
                     VVTAPIPVVSDLAGQPITLVDLAGMTGFVNPDLIYAIADVITLVNSIPDIDPEAPALI
                     LPFGNFTIYDTSSPDPSLQLSLWDPDFDQTDRNQITRPSFDPDYIAGQLDNLSNTAPP
                     QNQQAASFTNGLAGQDFGDFISFPIFQDPSQIFGLLMGNDISIIEID"
BASE COUNT         1136 a         2280 c         1990 g          958 t
ORIGIN      
        1 cccagggtgc tcaccggggt ttcggtcggc accggcacga tcccggccgg caaccccgag
       61 ggcgaagagg aagaagcgcc gcaggcgctg cccccgggct tcgaggcgct cgaagggatc
      121 cggcgcctga tcgagaccgg cgccggcgcg ttcaacctgg acgacatcgg ctccgccgcg
      181 atcgacacgt tcgacgagct gcgcgacgcg ctcgacgcgc tcgacggcga cgacggcaac
      241 gtcacgttcc agacggtcgg agacaccacg accttcacgg tcgagatcgt caaggagatc
      301 accggcaccg ccgacttcac cgtggaggcc gaggagttcg gcggcgagat cgacctcggc
      361 ggggtgatcg acatctccgc cgacgtcgtc ctcacgctcg agttcggctt cgacccgggc
      421 ggcttcttcg tcaacaccgg cgcgagcggc gaggagctga agctccacaa catcgtcctc
      481 gaaggcgacg cgcgggccgc cggccggttc ggcttcctcg acatcgaggc cgaggtcgag
      541 gccttcaccg tcgacgacgg catcgagctc gtcgtcgacc tgcaggaccc gatgggcgac
      601 ggcaagctgc gcgtgtcgga cttcggctcg agcctcggcg acaaggtcga ggtcacgctg
      661 aacaacccgg gggtcgacga cgtcacgctt gcggtcacgg cgagcgccgc ggccatcctg
      721 cccggcctgg aatcgatcga cctgggcggc gcgcagctta ccctcacgtg gcaggacatc
      781 accgatccgg gaacgctgga tatcaccgcc ggcggcgggc tggcgcagga cctgatcgac
      841 ttcctgagcg tcgacgtgca ggaagtcctc gaccaggtct cgcagctcga cgagctcgcc
      901 gacgcgctcg acggcttcga cgtgccgctg ctgcaggaca cgctgagcgc gctcgccgac
      961 gccgccgact tcatcagcga agagatcatc cagccgctca ccagcctgag cggcggccag
     1021 gcgagcttca acagcatcca gaatctgatc atgcgccttg cgcgctcgac gggcgtcgac
     1081 ccggccgcgc tcggactggc gtacgacgac ggcgagctca cctggcatct cgacttcggc
     1141 accggcttcg tcgcgtcgga cagcctcgac ctcggcttca actcggactc tacgggactc
     1201 gccgacctgt ccttcagcac cgacggcagc atcgagggca cggtgggctt cgagctcacg
     1261 gtcggcatcg acatcggcgc gatcgtgggc ggcgcgcccg ccgacgaatg gttcttcctg
     1321 cgggatgcgt cggcttccgc cacgctcgac atcgaggcaa ccgccatcaa tgccgaggcg
     1381 cgcttcggct tcctgtcgat cgggatcgag ggcggcaccg ccgaggcgga ggccggcttc
     1441 ctcatcacgc tgaacgaccc ggacaacacg gatggcaaga accgcatcac tctgagcgag
     1501 ctgctggacg ggctgagcag cccgggcgac ctcgtctcgt tcgatctgac cgggctcaat
     1561 gccgaaggcg acgccgaccc ggccacggac gcgaacctct cgctgacgct gccgctggtc
     1621 atcccgttcc tcgacgtgga tctcgacgac gaccagcagc tcatcctgct gtggtcgaat
     1681 ctcgaggacc cggacacgat ctcgctcgcg ctgcccgagg gcctgctcga aaacatcctg
     1741 ccggacttca ccaacatgga tgccggcacg ctcgtcagcc tgctcggcac catctcgaac
     1801 tggctcgagg acttccgccg caacttcgac gtcgcgaaca tccccttcgt cggcacggcg
     1861 ctcgacgagg cgctgcagtt cgccgacctg ttctcggaca cgctgctgtt cgacgaccac
     1921 gacgacgagg acgacgccac cttcaccgag aagctgctcg acgtcgacgg ccgcccgacg
     1981 ttcgacacgg tccagcagat ggtcggccgc ctggtcacga tcctcggcct gcccgaggac
     2041 caggtgttct acgatgacgc cgacgagacg atctacatca acctcgagat cggcgacgtc
     2101 gacagcgcgt cgaacttcgg cttcgtcgac ctgccgatca agttcgacct gctgcaggac
     2161 ctggcgccga tcgccgagct ctcgagcgac agcaccatcc gcctgtcggc gggcggcggc
     2221 atcacgctca ccttcggcat ctatctcggc aacgccggcg cggtcacgct caccaacgac
     2281 acgctgctcg ccgacctgaa gggcggcatc gccttcagcc aggcgcgcgt cgtcgccacc
     2341 ccgaacgagg tcaccaccgt ccacgggcgc ctgtcgggca acgccaagtt caagctctcg
     2401 gtgaacggcg gcgacgaaga agagatcgtc atcgacaacg acgacaccga ggacaacacc
     2461 tcggtgcagc acctggtcga cgacatcaac gacgcgctcg agctggccgg tttggacacg
     2521 caggttgtcg ccgagcaggc cagcgacgac ggcaatccga tcaacaggat caacctgagg
     2581 gcgctcggcg gcgtgagcac gctcacgctg agcgcgcagc aaggcgaccc ggcgatcacg
     2641 cagatcggct tcaaggcgag catgaccgcc gctcccgacc ccgacgacgg caaccagctc
     2701 aagatcaagg gccaggccga cgtgtcgggc gtcgtcggcc gcctgtcggg caacgccgtg
     2761 atgcaggtgt cgctcgacac cgtgaacggc ggaacgccgg tcatcgtaac ggtcgacgcc
     2821 gacgacacct ccaccaaccg caacatcctc gacgtcgtgg tcgacgtgca gaacgcgttc
     2881 gacgacgccg gcttcgagga cgagatcgag gtcacctcgc tcggcaagca cctcatcttc
     2941 cgtacgctca atcccgccgc gaccagcctg accatcaacg tcgcgggcgg caacccggcg
     3001 cagcagctcg gcctcgccac ctcgaacctc ggcaactcgc acgatcttct catcaccacc
     3061 agcaacggcg tcgtgcaccc gatcacattc gggccggaag tcgacacgct gggcgaggtg
     3121 ctgagcgcga tcagctcgca gaccggcggc gcggtcagcg ccagcttcag cgacaacgac
     3181 acccgcatcc tgctcaccga caacctggag cagacgaccg cggtgttcaa ggtgaacaac
     3241 gcggtcggct ccaccgcggc gttcgacctc gcgatcttcg gcttcgacac ggtcgagccc
     3301 gagcccgagg acgacccgcg cgacttccag atcgagggcg gcctgctcgg cggcgtcgat
     3361 ccgctcgatc gcctgttcgt ccagaacgcg caggcgcagg cgagcgtcgg gatcgtcact
     3421 ccccagctca acgaggacgg cgagatcgag gacaccgacc tcgacggctc gaccaccgac
     3481 gggctggatg ccgaggcgcg cttcggcttc gtgggcatcc acggccacgg cggcatctcc
     3541 aacctcgcgg gcgacggccc cctgacggcc tccatcgacg tcgcgctcaa gcccgcggac
     3601 gccgaggcgt tcgaccccga ggcgaagatc accctcaagg acctgatcga caacatcggc
     3661 aacatcggcg acttcctcga cgggcccacg atcgacggca gcggcaaggt cgagctcgcg
     3721 gtgacgatca ccccgagctt cagcgacatc gagaccgggg cggagccgac gctgatcatc
     3781 gagctcacca gcctggccga gatcctcgac ggcgacgaag acggtgacgc gggctacacc
     3841 atcgataccg agaacttcga ggatctgctc aacttcgacg agatcgggtt cgacgacatc
     3901 ctcgccgcgc tgcgcgcgct ggtcgacttc ctcgggcagt tcgaacagtt cggcttcctc
     3961 aacgacgaca tcccgctcat caacatcagc atcaacgaca tgctgtcgtt cgtcgaggac
     4021 ttcgaggagg cgctcgacga ggtgcaggac aacccggccg gcacggtgca ggtgctggag
     4081 accaagctca aggaggcctt cggcattccg cagtcgagcg acgtgctcca gctcgacctg
     4141 gtggacggca gcatcctgcg catcgacctc aatttcgagc cctcgttctc cgagtcgctc
     4201 ccggtcagcc tcgagctgcc gatcgagacc ggcccgatcg acctctcggg caacgccaac
     4261 ctgcgcgccg agggcgcgct cgacctcacc ctcgccttcg gcatcgacct cggcttcctc
     4321 accggcgagg acgatttcga catcgagaac gtgctctggg tgttcgagga caccggcatc
     4381 gcgggctcgc tcaccgccgg cgccgacgac atctcgttca ccgcggcgct cggcggcctc
     4441 ggcgcgcgca tcgtcgacgg cgaggccgag atcaccggcg cgttcaacct cgggctggcg
     4501 gcgagcgcca tgaccgcggg caccggccag aaccggcgca tctcgttcac cgacctgatc
     4561 ggcgacatcc ccggctcgat cgacgccagc ctcaccggcg aagtcacggc cctgctgccg
     4621 gtgtacttcc cgaccgagtc gagcttccgc ggcaacatcg aggtcggcgg cgcgctcgag
     4681 ctcacgcccg agaacgggct cgagctcaac ggcaccctgg tcggcgagga cgtggtcctc
     4741 gacggcttcg tgcgcattcc ggacgagatc ttcagcctcg acttcagcca gttcagcgcg
     4801 ctcgacaacc tgctcttgat catcgacggg gtcgacgggt tcctcggcct gctgcaggac
     4861 ctgttctccg gacaggtcgg cgggttcacg ctgccgctgg tcggcgacca gctcgccgag
     4921 gccgccgacg tgatcgagga cttccgcaag gacttcatcg acgggctgcg cgacgcggtc
     4981 gagaccgcgg ccgacccgga ccagaactac atctcggagc agctgttcgc gctgctcggg
     5041 ccctcgggcc tgaatatcct gctcgacggc gacgacgcgg gcagcgacgt gaatgtcggc
     5101 gacatcgtgc tcgacacgaa tgtcgacgaa ggcggcgagg gcgaagagcc gcccccgccc
     5161 gaagaaatct tcatgcagtg gaacatgcgc ctcggcagca cgctgctcaa cgccggcgag
     5221 gggctcgact tcgacatcgg gatccccggg ctgggcctgg agacgcgcgg ccggatcgag
     5281 gtcgagatcg actgggagct cgcgctcggc ttcggcgtcg gcatctcgcg cggcggcttc
     5341 tacatcgata cctcggacct gaacgagttc gaggtcgagg ccgaggtcac gctgcccgac
     5401 gcggggctga ccggcaggct cctgttcctc cagctcgacg ccgacaacaa cggcaacacc
     5461 ggcctgggcg ccacgttcgg cgtcgacatc ttccgcgaag gcggcggcga tccgctgatc
     5521 ggcatcgccg atctcggcag cctcggcatc gacatcggcg tcgccgccca cgcgaacatc
     5581 gacctcggcc tggagctcaa gctcaacagc gagctggtgc cgaactcgat cagcaaggtg
     5641 ttcccgaaga tcgtcggcga tttcgtgctc gatttcgaga tcggcgattt caccgacccc
     5701 gaaaacccgg tgctggtcgg cttcgacgag atcggcaacg cgttcgagga gggcctgaag
     5761 cgggtcgcgt tcgagaacgt cggcctcgac ctcggcagct tcatctccga cttcctcggg
     5821 ccggtcgtcg acgaggtgca gaagttcacc gggccgatcc agccgatcat cgacgtggtc
     5881 accgcgccga ttccggtggt gtccgacctc gccgggcagc cgatcacgct ggtcgacctc
     5941 gccgggatga ccgggttcgt caaccccgat ctgatctacg ccatcgccga cgtcatcacg
     6001 ctggtcaaca gcatccccga catcgacccc gaagccccgg cgctgatcct gccgttcggc
     6061 aacttcacca tctacgacac gtcgtcgccg gacccgagcc ttcaactgag cctctgggac
     6121 ccggatttcg accaaacgga ccgcaatcag atcacgcggc cgagcttcga tcccgactac
     6181 atcgcgggcc agctcgacaa tctgtcgaat accgcgccgc cgcagaacca gcaggccgcc
     6241 tcgttcacca acggcctcgc cgggcaggac ttcggcgact tcatcagctt cccgatcttc
     6301 caggacccga gccagatctt cggcctgctg atgggcaacg acatctcgat catcgagatc
     6361 gacc
//
LOCUS       DASPFR010000323         7519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_704011, whole genome shotgun sequence.
ACCESSION   DASPFR010000323 DASPFR010000000
VERSION     DASPFR010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7519
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704011"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1309)
                     /locus_tag="VD737_08525"
     CDS             complement(38..1309)
                     /locus_tag="VD737_08525"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HXW10645.1"
                     /translation="MTDHESLAATMQALGRDAVAAQRELALADGTRRTRALQAMAAAL
                     RAQQQQILAANERDMSAAAEAGLGHAGLDRLRLDATRVEAMARGVEEIAAMPDPIGAV
                     MAEWTRPNGLRIQRVRVPLGVIGIVYESRPNVTCDAGALCLKSANAAILRGGSESHHS
                     STAIHRCLAEGLRAAGLPDACIQLVPTTDRAAVGMMLTGLDGAIDVMVPRGGKSLIAR
                     VQREARVPVIGHLEGICHVYVDKHANLEMARSIVLNAKLRRTGVCGAAETLLVDAAAE
                     RTHLAPLVRELLDVGCEVRGDAAARAVDPRVVAATEQDWRTEYLDAIISVKLVDGVDG
                     AIEHVERYGSDHTESIVTDDAATAEQFLARVDSAIVLHNASTQFADGGEFGMGAEIGI
                     STDRFHARGPVGVEQLTSYKYVVRGAGQVRP"
     gene            complement(1306..2448)
                     /gene="proB"
                     /locus_tag="VD737_08530"
     CDS             complement(1306..2448)
                     /gene="proB"
                     /locus_tag="VD737_08530"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HXW10646.1"
                     /translation="MSTPSAPRASSRLARARRVVVKVGSALLVERSTGRVNRAWLETL
                     AADVAALRARGQEVVLVSSGAIALGRRHLGLGSGRLRLEESQAAAAVGQIRLAHAWKE
                     VLEQHECTVAQVLVTLGDTEVRRRYLNARNTLETLLRLGAIPVINENDTVATAEIRYG
                     DNDRLAARVAQMASADCLVLLSDVDGLYTGDPTLDPAAAFIPEVTRITPEIEAMAGGS
                     ASDVGSGGMATKIMAGKIALAAGCSMCIAIGREPHPLQRLEAGARCTWFIAEASPRTV
                     RKQWIAGSLKPAGTLYVDEGAAGALRDGKSLLPAGLTRVEGRFDRGDAVSVRDPAGTE
                     LARGLSAYSSEDARRVCGRRSRELEAILGYRGRDEIIHRDDLVLLT"
     gene            complement(2445..4241)
                     /locus_tag="VD737_08535"
     CDS             complement(2445..4241)
                     /locus_tag="VD737_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HXW10647.1"
                     /translation="MYSYRAPLRDIRYAVHEVLDFEDHYRAYGREDLTRDLLEGILEE
                     GARFAEDVLAPTNRVGDEHGLVFEDGQVRTPPGFKEAYERYCQDGWSTMVFDTELGGQ
                     GLPAGFSLAFGEMLVSGNMAWRMYSGLTESASLTIAAHAGEALRQRFLPKMITGEWSG
                     TMCLTEAHSGTDLGILKSRAEPQADGSFRVTGTKIFISAGEHDLTSNIVHLVLARLPD
                     APPGTRGISLFLVPKFLPDANGDAGEPNGVTCGSIEHKMGIHASPTCVMHFNDSTGWL
                     VGKPNEGLACMFTMMNHARLGVGLQGHGLSELAWQASIAYANDRLQGRAMTGPKEPGK
                     PADPIIVHPDVRRMLLTQKAFVEGGRLLCFLTGREIDSAHLNPDETERRRSNDLIAFL
                     TPIVKGFLTEAAMEVTSLAVQIHGGHGFIRQTGVEQLMRDARITPIYEGTNGVQALDL
                     LGRKVFGTGGKSQQMISARMVDSIQKFGSLPEVAPFAAELGKRLEQWGKLTAELGAAA
                     MKNPEEVGAAATDYLHIAGYMCLGWCWLVSAGVAASRLAANPDDDFYKAKLVTARHYF
                     TRLFPRVDSHMTAARAGSAGLMALTADQFAIA"
     gene            4404..4754
                     /locus_tag="VD737_08540"
     CDS             4404..4754
                     /locus_tag="VD737_08540"
                     /inference="COORDINATES: protein motif:HMM:NF042799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6763 family protein"
                     /protein_id="HXW10648.1"
                     /translation="MSRDYEPQVGQWYEDLDTEETFQVLSLDPDEEIIRIQWPDREVQ
                     ELDLDQWNEMDLELAVAPEGWVDDVEESDEDDDEVDLEDEDDWEEDEDEDEDDDDWDD
                     DEDDLDDDDYSDRE"
     gene            4751..5347
                     /locus_tag="VD737_08545"
     CDS             4751..5347
                     /locus_tag="VD737_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXW10649.1"
                     /translation="MSPAPDAPPSPARPPLPGRYPTELERTWTPAGRPPVQLRALRPD
                     DIELELQFVERLSADTLHLRLQSFATEVSRRDLERLLDTDYVNSLAVGGFVDANGAVA
                     LVGVSRYARIEGTTRAECAIVVADDWQGCGLGTELMRSLAQAAVARGYTCLEGETLAE
                     NSRLATWARRFGFNVKTQPYSGGLVKVTIELDQLLPVQ"
     gene            complement(5355..5684)
                     /locus_tag="VD737_08550"
     CDS             complement(5355..5684)
                     /locus_tag="VD737_08550"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXW10650.1"
                     /translation="MTQQASVSPPPTRHWTFLSNHGHVLVCLALDPKARLRDVAASVG
                     ITERAVQKIVGDLEAAGVVVRQRSGRRNTYRLNASVPLRHPLEAHRSVGTLLSLVVER
                     SGRGRTA"
     gene            5881..6765
                     /gene="htpX"
                     /locus_tag="VD737_08555"
     CDS             5881..6765
                     /gene="htpX"
                     /locus_tag="VD737_08555"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HXW10651.1"
                     /translation="MKRIFLFLATNLAIVVLLGIVLQLLGINSILDAQGSDLDLGALL
                     VMSAVVGFTGSLISLAISKWTAKRMMGVRVITQPANSMESWLVSTVQRQAQAAGIGMP
                     EVGVFDSPEPNAFATGARRDAALVAVSTGLLQRMSQREAEAVLGHEVSHVANGDMVTL
                     TLIQGVVNTFVFFLSRVIGHVVDRVVFKTEEGHGPAFLITSIIAQIVLGLFATMIVLW
                     FSRQREFRADSGGARLAGREHMIGALERLQQTHAGPLPEKMAAFGISGPVASGLKRLF
                     MTHPPIQERIAALRASVG"
     gene            7088..>7519
                     /locus_tag="VD737_08560"
     CDS             7088..>7519
                     /locus_tag="VD737_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HXW10652.1"
                     /translation="MNQQTRSTAVVWFRRDLRLADNPALAMAARHADHVVAVYVHAPE
                     EEDAWRPGAASDWWLHLSLARLDAALRRCGIALTLRRGPSSAHALAEIARESGATHLY
                     WNRLYEPAVAERDRRVEAALTASGIACESFNGGLLCEPQDLL"
BASE COUNT         1115 a         2629 c         2610 g         1165 t
ORIGIN      
        1 agaccggcac agtcgccggg tatcacggcg gcctgcgtca gggtcggacc tgaccggcgc
       61 ctcgcacgac gtacttgtag ctcgtcagct gctcgacgcc gaccgggccg cgcgcgtgga
      121 accgatcggt cgagatgccg atctccgcac ccatgccgaa ctcgccgccg tcggcgaact
      181 gcgtcgaggc gttgtgcagc acgatcgcgc tgtcgacgcg cgccaggaac tgctcggcgg
      241 tcgcggcgtc gtccgtgacg atcgactccg tgtgatcgga gccgtagcgc tcgacgtgct
      301 cgatcgcgcc gtccacgccg tcgacgagct tcacggagat gatcgcgtcc aggtattcgg
      361 tgcgccagtc ctgctccgtg gcggcaacca cgcgcggatc gaccgcgcgt gccgccgcgt
      421 cgccgcgcac ctcgcagccg acgtcgagca gttcgcgcac gagcggggcg aggtgcgtac
      481 gctcggccgc tgcatccacc agcagcgtct cggcggcacc acagacgccg gtgcggcgca
      541 gcttcgcgtt cagcacgatc gagcgcgcca tctcgaggtt ggcgtgcttg tccacgtaga
      601 cgtggcagat gccctcgagg tgcccgatca cgggcacgcg cgcctcccgc tgcacgcgcg
      661 cgatcaggct cttgccgccg cggggcacca tcacgtcgat cgcgccgtcc aggccggtca
      721 gcatcatccc gaccgccgcg cgatcggtgg tcggcacgag ctggatgcag gcgtcgggca
      781 gaccggcggc acgcaggccc tcggccaggc accggtggat cgcggtgctg gaatgatggc
      841 tctcgctgcc gccgcgcagg atcgcggcat tggcggactt caggcacagc gcgcccgcgt
      901 cgcaggtcac gttggggcga ctctcgtaga cgatgccgat cacgccgagc ggcacgcgta
      961 cgcgctggat gcgcaggccg ttcgggcgcg tccactcggc catcacggcg ccgatcgggt
     1021 cgggcatcgc cgcgatctcc tcgacgccgc gcgccatcgc ctcgacgcgc gtcgcgtcga
     1081 gcctcagccg gtcgaggccg gcgtgcccga gccctgcctc cgccgcagcg ctcatgtcgc
     1141 gctcgttggc cgccaggatc tgctgctgct gcgcgcgcag cgcggcggcc atcgcctgca
     1201 gggcgcgcgt gcggcgcgtg ccgtcggcca gcgccagctc gcgctgcgcc gcgacggcgt
     1261 cgcgccccag cgcctgcatg gtcgcggcga gtgattcgtg gtcggtcacg tcaacagcac
     1321 caggtcgtcg cggtggatga tctcgtcgcg gccacgatac cccaggatcg cctcgagctc
     1381 gcggctgcgg cgtccgcaga cgcggcgcgc gtcctcgctc gagtaggcgc tcagcccgcg
     1441 cgccagctcg gtgccggccg ggtcgcgcac gctcacggcg tcgccgcgat cgaagcgtcc
     1501 ctcgaccctc gtgaggcccg cgggcagcag gctcttgccg tcgcgcagcg cgccggcggc
     1561 gccttcgtcg acgtacagcg tgccagcggg cttgagcgac ccggcgatcc actgcttgcg
     1621 cacggtgcgc ggcgaagctt ccgcaatgaa ccaggtgcag cgtgcacccg cctccaggcg
     1681 ctgcagcgga tgcggctcgc gaccgatcgc gatgcacatg ctgcagccgg ccgcgagcgc
     1741 gatcttgccg gccatgatct tggtcgccat gccgccggaa ccgacgtccg acgccgagcc
     1801 gcccgccatc gcctcgatct ctggcgtgat gcgcgtgact tccgggatga acgccgccgc
     1861 cggatcgagc gtcgggtccc ccgtgtacag gccgtccacg tcggagagca gcacgaggca
     1921 gtccgcgctc gccatctgcg cgacgcgcgc ggcgaggcgg tcgttgtcgc cgtagcggat
     1981 ctcggcggtc gcgaccgtgt cgttctcgtt gatgaccggg attgcgccga gcctgagcag
     2041 ggtctcgagc gtgttgcggg cgttcaggta acgccgacgc acctcggtgt caccgagcgt
     2101 gaccagcacc tgcgcgacgg tgcactcgtg ctgctcgagc acttccttcc aggcgtgcgc
     2161 gagccggatc tgcccgaccg cggcggccgc ctggctttcc tcgagacgca gccggcccga
     2221 cccgagtccg aggtgtcgtc gaccgagcgc gatcgcgccc gaggacacca gtacgacctc
     2281 ctgcccgcgc gcacgcaacg ccgcgacatc cgcggcgagc gtctcgagcc aggcgcggtt
     2341 gacgcggccc gtcgaacgct cgacgagcag tgcggacccg accttgacca ccaccctccg
     2401 cgcccgggcg agtcgcgaac tggcccgggg cgcggaaggc gtgctcatgc gatcgcgaac
     2461 tggtcggccg tcagcgccat caggcccgca gaaccggcac gggcggcggt catgtgcgaa
     2521 tcgacgcgcg ggaacagccg cgtgaaatag tgccgcgccg tgacgagctt cgccttgtag
     2581 aagtcgtcgt ccgggttcgc ggcgagcctc gaagcggcga cgccggccga gaccagccag
     2641 caccagccga ggcacatgta gcccgcgatg tgcaggtagt cggtcgcggc ggcgccgact
     2701 tcctcagggt tcttcatcgc cgccgcgccg agctcggcgg tgagcttccc ccactgctcg
     2761 aggcgcttgc cgagctcggc cgcgaacggc gccacctccg gcagcgaccc gaacttctgg
     2821 atggagtcga ccatgcgcgc cgagatcatc tgctggctct tgccgccggt gccgaagacc
     2881 ttgcggccga gcaggtcgag cgcctgcacg ccgttggtgc cctcgtagat cggcgtgatg
     2941 cgcgcgtcgc gcatcagctg ctcgacgccc gtctggcgga tgaagccgtg gccgccgtgg
     3001 atctgcacgg cgaggctcgt gacttccatc gccgcctcgg tcaggaagcc cttcacgatc
     3061 ggcgtcagga acgcgatcag gtcgttcgag cgccggcgct ccgtctcgtc cgggttcagg
     3121 tgggcgctgt cgatctcgcg gcccgtcagg aagcagagca ggcgcccgcc ttcgacgaag
     3181 gccttctgcg tgagcagcat gcgccgtacg tcggggtgca cgatgatcgg gtcggcgggc
     3241 ttgcccggtt ctttcggccc cgtcatcgcg cgaccctgca ggcgatcgtt ggcgtaggcg
     3301 atcgacgcct gccacgcgag ttcggacagg ccgtggccct gcaggccgac gccgaggcgc
     3361 gcgtggttca tcatcgtgaa catgcaggct aggccttcgt tcggcttgcc gaccagccag
     3421 cccgtcgagt cgttgaagtg catcacgcag gtcggcgagg cgtggatgcc catcttgtgc
     3481 tcgatcgaac cgcaggtgac gccgttcggc tcgccggcgt ccccgttcgc gtccggcagg
     3541 aacttgggca cgaggaacag gctgatgccg cgcgtgccgg gcggcgcgtc ggggagccgc
     3601 gccagcacga ggtgcacgat gttggaagtc aggtcgtgct caccggcgct gatgaagatc
     3661 ttggtgccgg tcacgcggaa gctgccgtcg gcctgcggct cggctcgtga cttgaggatg
     3721 cccaagtcgg tgcccgagtg cgcttcggtc aggcacatcg tgcccgacca ttcgccggtg
     3781 atcatcttcg gcaggaaccg ctgcctcagt gcctcgccgg cgtgcgcggc gatcgtgagc
     3841 gaggcgctct cggtcagccc ggaatacatg cgccatgcca tgttgcccga gaccagcatc
     3901 tcgccgaacg cgagcgagaa gcccgccggc aggccctgcc cgcccagttc ggtatcgaac
     3961 accatcgtgc tccagccatc ctggcagtac cgctcgtagg cttccttgaa gccgggcggc
     4021 gtgcgcacct gcccgtcctc gaacacgagg ccgtgctcgt cgcccacgcg gttggtcggg
     4081 gccagcacgt cctcggcgaa gcgtgcgcct tcctcgagga tgccctcgag caggtcgcgg
     4141 gtgaggtcct cgcggccgta ggcgcggtag tgatcctcga agtccaggac ctcgtggacg
     4201 gcgtagcgga tgtcacggag cggtgcgcga taggagtaca cgatgacgac ctctggcagc
     4261 ccggtttctc cccgagtgta cgcgaattgc ggcagtgcag cataggtgag ttccggttgc
     4321 cgcgccgttc cggccgaccg tatagttatt cgcattcgca ggccgttcgc gcggccgttt
     4381 tttttcgccg aggtaaatcg accgtgagcc gtgattacga accgcaggtc gggcagtggt
     4441 acgaagacct cgacaccgaa gagacgttcc aggtcctgtc gttggacccg gacgaagaaa
     4501 tcattcgcat ccagtggccc gatcgcgagg tgcaggagct cgacctcgac cagtggaacg
     4561 agatggatct cgaactggcc gtggcgccgg aaggctgggt cgacgacgtg gaagaatccg
     4621 atgaggacga cgacgaggtc gacctcgagg acgaggacga ctgggaagaa gacgaggacg
     4681 aagacgagga cgacgacgac tgggacgacg acgaggacga tctcgacgac gacgattaca
     4741 gcgaccgcga gtgagccccg cgcccgacgc cccgccctcg cccgcccggc cgccgctgcc
     4801 gggacgctat ccgactgaac tcgagcgcac ctggacgccg gccgggcgcc cacccgtcca
     4861 gctgcgtgcg ctgcgccccg acgacatcga actcgaactg cagttcgtcg agcgactgtc
     4921 cgcggacacg ctgcacctgc gcctgcagag cttcgccacc gaggtctcgc ggcgtgacct
     4981 cgagcgcctg ctcgacaccg actacgtgaa cagcctggcg gtcggcgggt tcgtcgacgc
     5041 gaacggcgcc gtggcgctcg tcggcgtcag tcgctacgcg cggatcgagg gcacgacgcg
     5101 cgccgagtgc gccatcgtgg tggccgacga ctggcagggg tgcggcctcg gcaccgaact
     5161 gatgcgcagc ctcgcgcagg cggccgtcgc gcgcggctac acgtgcctcg agggcgagac
     5221 gctggccgag aattcacgcc tcgcgacctg ggcacggcgc ttcgggttca acgtgaagac
     5281 gcaaccctat tcgggcgggc tcgtgaaggt gacgatcgaa ctcgaccagt tgctgccggt
     5341 gcagtgacga tcgttcaagc ggtgcgccca cgaccgctcc gctcgacgac cagcgacagc
     5401 agcgtgccga ccgagcgatg cgcctccagc gggtgccgca gcggcacgga cgcgttcagg
     5461 cggtaggtgt tgcggcgacc ggatcgctgg cggaccacga cgccggcggc ctcgaggtcg
     5521 cccacgatct tctggaccgc gcgctcggtg atgccgacgc tggcggccac gtcgcgcagg
     5581 cgcgccttgg ggtcgagcgc caggcagacc agcacgtggc cgtggttgct caggaaggtc
     5641 cagtgccgcg tcggcggcgg cgaaacggat gcctgctgcg tcacgaatca gcctccatgg
     5701 aaccggagcc ggccgtcatt gtctgaagct gggttccggt gttttggttc ggtgttagta
     5761 tgagccaagc cgggtcgtcg gcgtaccgcg cgaccgtcgg ccgcttgaat cccgccacct
     5821 catccccacc gaatgatccg aaacgggcgc tgcggggccc gtatggagtc tgaagcccac
     5881 atgaaacgca tcttcctgtt cctggccacc aacctggcga tcgtcgtgct gctcggcatc
     5941 gtgctgcagt tgctcggcat caacagcatc ctcgacgcgc agggctcgga cctcgacctg
     6001 ggcgcgctgc tggtgatgtc ggccgtggtc ggtttcaccg gttcgttgat ctcgctcgcg
     6061 atctccaagt ggaccgccaa gcgcatgatg ggcgtgcgcg tgatcacgca accggccaac
     6121 tcgatggaga gctggctcgt cagtaccgtc cagcggcagg cgcaggcggc cggcatcggc
     6181 atgcccgaag tcggcgtgtt cgactcgccc gagccgaacg ccttcgcgac cggcgcgcgg
     6241 cgcgacgcgg cgctggtcgc ggtgagtacg ggcctgctcc agcgcatgag ccagcgcgag
     6301 gcggaagccg tgctcgggca cgaagtcagc cacgtcgcca acggcgacat ggtcacgctc
     6361 acgttgatcc agggcgtggt caacacgttc gtgttcttcc tgtcgcgagt gatcggccac
     6421 gtcgtcgacc gcgtggtctt caagaccgag gaaggccacg ggccggcgtt cctgatcact
     6481 tcgatcattg cccagatcgt actgggcctg ttcgccacca tgatcgtgct gtggttctcg
     6541 cgccagcgtg agttccgcgc cgactcgggc ggcgcgcggc tggccggtcg cgagcacatg
     6601 atcggcgcgc tcgagcgtct gcagcagacc cacgcgggcc cgctgcccga gaagatggcg
     6661 gcgttcggca tctccggacc ggtcgccagc gggctcaagc ggctgttcat gacgcacccg
     6721 cccatccagg agcgcatcgc cgcgctgcgc gcctcggtcg gctgacgtcg acgcggcgca
     6781 tccaacgcgc cgccctgcgc gaaaccaagg ccgcgccttc tcggaggcgc ggctttgttt
     6841 tttcttgtcg cggcggcgtg tggccaccgg cacgccgccg ctgatcctga aagctgaccg
     6901 ccgcgccagg ggtgctgcgg tagcggccgc tgagggctgc ccgccgcatt tcgagccggg
     6961 gagacgccgt aagaggcatc cctgccggct cgcgctcgcc gtccctggct cgcgacgctc
     7021 cccgactcga aatccggcgg caaccctcgc ggcggtcggc gcgcgttgaa acgtcgagat
     7081 gcacaccatg aaccaacaaa ccagatcaac tgccgtcgta tggtttcgac gcgacctgcg
     7141 cctggccgac aacccggcgc tggcgatggc cgcgcgccat gccgaccacg tcgtggccgt
     7201 ctacgtgcac gcgccggagg aagaagacgc ctggcgtcct ggcgccgcga gcgactggtg
     7261 gctgcacctc agcctcgccc gcctcgatgc agccctgcga cgatgcggca tcgcgctgac
     7321 gctgcgacga ggaccatcgt cagcgcacgc actggccgag atcgcgcgcg agagcggcgc
     7381 cacgcacctg tactggaacc gcctctacga acctgcggtc gcggagcgcg atcgacgcgt
     7441 cgaggcggca ctgaccgcgt ccggcatcgc atgcgaaagc ttcaacggcg gattgctctg
     7501 cgagccgcag gacctcctg
//
LOCUS       DASPFR010000324         4008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1071017, whole genome shotgun sequence.
ACCESSION   DASPFR010000324 DASPFR010000000
VERSION     DASPFR010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071017"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1327
                     /locus_tag="VD737_08565"
     CDS             <1..1327
                     /locus_tag="VD737_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXW10653.1"
                     /translation="NAYDPTTGLLTRPAFEKRAHAVLAAGQGGNHCVVYVDIDRLHVL
                     NENLGMHVGDEVIVRVAEAIRRSVTPRMLASRISGDRFALLLPDANVDRAQDLAERLR
                     DSLERLGFVRDRQTVDVTASFGIAAVMGGHLSLSHALASAEIACKAAKDRGRNRVEVY
                     AEGDQSIVRRYTDLTLVGTVRSALLDERFRLEAQPIVPLNGAAPHPKFELLLRMTDEA
                     GTSVPPDKFLSAAERYQLAPAIDRWVVRRVIETLRPLATLLQQRQACFAVNISGQSLG
                     DADFAVFMESTLREARLPLELLSFEVTETAAVANIVRAEALIRRLRELGCAVALDDFG
                     RGLSSLTYLKTLPVNYLKIDGSFVRDVVADERSHAMLSAIVQLARAMGLETVAECVES
                     DEIRQAIQALGVSYGQGFSIGRPVPLDKVLAALVAANITAREPLTAVSK"
     gene            1324..2610
                     /locus_tag="VD737_08570"
     CDS             1324..2610
                     /locus_tag="VD737_08570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXW10654.1"
                     /translation="MNRRLSASLALALGAAVGFAAAHLPGLFRAEPAALAAVPPAEAL
                     PGGLATHRLLDEVVNRIQREYVDEVPVRAFDEAAVEGIVANLDPHSAFLDAREYDEMR
                     ATTTGSYSGVGIEVSAEDGRVVVVTPIEGSPAARAGVRSGDVVLAVDGRPVTADRLEE
                     TIGRMRGAVGTRVRLAVGRAGEAEPLTFTLERGEVHVHTVRAEPLPGEFGYVRITQFS
                     DSTPDDLLAALTRLMRTPGDPSLHPMQGLVLDLRGNPGGVLESAVSVADAFLEDGLIV
                     RADGRTPEARFQMAATAGDVLDGQPIVVLVDGGSASGSEIVAGALRDHGRATLMGERT
                     FGKGSVQTVIPLRDGQALKLTTSRYFTPSGTSIHERGLEPDIVIRRLEDEPAVVRPVP
                     PMQDSAVLTALQYLRDRGLGTHYAAASAAGPASSAH"
     gene            complement(2596..2985)
                     /locus_tag="VD737_08575"
     CDS             complement(2596..2985)
                     /locus_tag="VD737_08575"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HXW10655.1"
                     /translation="MEPVAIVSVLALLQYVAFGTAVARARGRYGVRAPAVTGHEVFER
                     YFRVQQNTLELLILLLPSLWLFARYVDAAWATVLGGVYLVGRTLYFIGYVRDPARREL
                     GFALSLGPTLVLLVGALIGACADLMGA"
     gene            3089..3469
                     /locus_tag="VD737_08580"
     CDS             3089..3469
                     /locus_tag="VD737_08580"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HXW10656.1"
                     /translation="MVGFLLRAAITALALWVASQLLGGMHFTTTGKLALAAVLLGLVN
                     AVVRPIAFILTLPITVVTLGLFLLVLNAGMVGLVAWLVPGFTISGFWTALGASVIVSI
                     VSWAASSAIGTSGRVEIIRGGTRD"
     gene            complement(3476..>4008)
                     /locus_tag="VD737_08585"
     CDS             complement(3476..>4008)
                     /locus_tag="VD737_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXW10657.1"
                     /translation="AQKISLPSGIGMLLGFGGVALLVWPENLLETGRIGWQIVVLLGC
                     LSWAIGTVQYRNVLLGLGPIAFNAAIMLLGSVILLVGGLARGELPQWHWEPTGMLALL
                     YLAVMSSAVAYTAFTWLLKNVRTDRVATFAYVNPAIATLLGWLVLGESMSPRQIVGTL
                     VILGSVALVTLPARFA"
BASE COUNT          606 a         1426 c         1384 g          592 t
ORIGIN      
        1 gaacgcgtac gacccgacga ccggcctgct gacgcggcca gccttcgaga agcgggcaca
       61 cgccgtgctc gccgccggcc agggcggcaa ccactgcgtc gtctacgtcg acatcgaccg
      121 gctgcacgtg ctgaacgaga acctcggcat gcacgtgggc gacgaggtca tcgtgcgcgt
      181 cgccgaggcc atccggcgca gcgtgacgcc gcggatgctc gcctcgcgga tctccggcga
      241 ccgttttgcc ctgctgttgc ccgacgccaa cgtcgatcgc gcccaggacc ttgccgagcg
      301 cctgcgcgac tcgctcgagc ggctcggctt cgtgcgcgac cggcagacgg tcgacgtgac
      361 ggcgagcttc ggcatcgccg ccgtcatggg cggtcacctg tccctgtcgc acgcgctcgc
      421 gtcggccgaa atcgcctgca aggccgccaa ggatcgcggt cgcaaccggg tcgaggtgta
      481 cgccgagggc gaccagagca tcgtgcgccg ttacaccgac ctgacgctgg tcggtaccgt
      541 tcgctcggcg ctgctggacg agcgcttccg gctggaggca cagccgatcg tgccgctcaa
      601 cggcgcggca ccgcacccga agttcgagct gctgctgcgc atgaccgacg aggccggcac
      661 cagcgtgccc cccgacaagt tcctgtcggc ggccgagcgt taccagctcg cgccggccat
      721 cgaccgctgg gtcgtgcgcc gcgtgatcga gacgctgcgc ccgctcgcga cgctgctgca
      781 gcagaggcag gcgtgtttcg cggtcaacat ctccggccag tcgctgggcg acgccgactt
      841 cgcggtgttc atggagagca cgctgcgcga ggcgcgactg ccgctcgaac tgctgtcgtt
      901 cgaggtgacc gagacggccg ccgtcgccaa catcgtgcgc gccgaggcgc tgatccgccg
      961 cctgcgcgaa ctcggctgtg cggtggcgct ggacgacttc gggcgcggcc tgagttcgct
     1021 gacgtacctg aagacgctgc cggtcaacta cctcaagatc gatggcagct tcgtccgcga
     1081 cgtcgtcgcc gacgaaaggt cccacgcgat gctgagcgcc atcgtgcagc tggcgcgcgc
     1141 gatggggctg gaaacggtgg cggagtgcgt ggagagcgac gagatccgcc aggcgatcca
     1201 ggccctcggc gtgtcctacg ggcagggatt ctcgatcggc cgcccggtac cgctcgacaa
     1261 ggtgctcgcc gcgctcgttg cggctaacat caccgccaga gagccgctga ccgccgtatc
     1321 gaagtgaacc gacgcctttc cgcctcgctg gcgctggccc tcggcgccgc cgtgggcttt
     1381 gctgccgcgc acctgcccgg gctgttccgc gccgagcccg cggcattggc cgccgttccg
     1441 cccgcggagg cgcttccggg cggcctcgcc acgcaccgcc tgctggacga ggtcgtcaac
     1501 cggatccagc gcgaatacgt cgacgaagtc cccgtgcgcg cgttcgacga agcggcagtc
     1561 gagggcatcg tcgcgaacct cgacccgcac tccgccttcc tggatgcccg cgagtacgac
     1621 gagatgcggg ccaccaccac cggcagttat tcgggggtcg gtatcgaggt ctccgccgag
     1681 gacggtcgcg tcgtcgtggt cacgccgatc gaagggtcgc cggccgctcg cgcgggcgtg
     1741 cgctcaggcg acgtcgtgct cgcggtggac ggccggccgg tcactgccga ccggctcgag
     1801 gaaacgatcg gccgcatgcg cggcgcggtc ggcacccggg tccggctcgc ggtcggccgc
     1861 gccggcgagg cggagccgct cacgttcacg ctcgagcgcg gcgaggtcca cgtgcacacc
     1921 gttcgcgccg agccgctccc cggcgagttc ggttacgtgc ggatcacgca gttcagcgac
     1981 tcgacgccag acgacctgct ggccgcgctc acccgcctga tgcgtacgcc gggcgacccg
     2041 agcctgcacc cgatgcaggg cctggtgctg gacctgcgcg gcaacccggg cggcgtgctc
     2101 gagtccgccg tgagcgtcgc cgacgccttc ctcgaggatg gcctgatcgt tcgcgcggac
     2161 gggcgcacgc cggaggccag gttccagatg gcggccacgg ccggggacgt gctcgacggc
     2221 cagccgatcg tggtgctggt cgatggcggc tcggcctccg ggtcggaaat cgtcgcgggg
     2281 gcgctgcgcg accacggccg tgccaccctg atgggcgagc gtacgttcgg caagggctcc
     2341 gtgcagacgg tgatcccgct gcgcgacggc caggccctca agctcacgac gtcgcggtat
     2401 ttcacgccgt cgggcacctc gatccacgag cgcggcctcg agcccgacat cgtgatccgg
     2461 cggctcgagg acgaaccggc cgtcgtgcgg cccgtgccac ccatgcagga ctccgcggtg
     2521 ctcacggcgc tgcagtacct gcgcgaccgc ggcctcggca cgcattacgc ggcggcttca
     2581 gcggcgggcc cggcctcaag cgcccattag gtccgcgcac gcgccgatca gggcgcccac
     2641 cagcagcacc agcgtgggtc ccaggctgag cgcgaagccg agctcgcgcc tggccggatc
     2701 gcggacgtaa ccgatgaaat acagagtccg tccgaccagg tagacgccgc cgaggacggt
     2761 cgcccaggcc gcgtccacgt accgggcaaa cagccacagc gacggcaaca gcaggatgag
     2821 cagctcgagc gtgttctgct ggacgcggaa gtagcgttcg aagacctcgt ggccggtcac
     2881 agccggagcc cgcacgccgt agcggccgcg cgcgcgggcg acggccgtgc cgaaggcgac
     2941 gtactgcagc agcgcaagca ccgagacgat ggcgacgggt tccatgcagc gatccgcgca
     3001 agtggcagaa ggtccgcgaa gattccttta ccatccgcgg caggtcaacc gcggtccgct
     3061 ccgccgtacc gcagacgagg ggacgttcat ggtcggattc ctgctgcgcg ccgcaatcac
     3121 ggcgctggcg ctctgggtcg cgagtcagct gctcggcggc atgcacttca cgacgacggg
     3181 caagctcgcg ctcgccgcgg tgctgctcgg cctggtcaac gcggtcgtcc ggccgatcgc
     3241 gttcatcctg acgctgccga tcacggtcgt cacgctcggc ttgttcctgc tcgtgttgaa
     3301 cgcgggcatg gtcggcctgg tcgcgtggct cgtgccgggc ttcacgatct ccggcttctg
     3361 gaccgcgctc ggcgcctcgg tcatcgtgag catcgtgtcg tgggcggcgt ccagtgcgat
     3421 cgggacctcg ggtcgtgtcg aaatcattcg tggtggcaca cgcgactgac tcgcctcagg
     3481 cgaatcgcgc cggtagcgtg accagcgcca cgctgccgag gatgaccagc gtgcccacga
     3541 tctgccgcgg actcattgac tcaccgagga ccagccagcc cagcaacgtg gcaatggctg
     3601 ggttcacgta ggcaaacgtc gccacgcgat ccgtgcgcac gttcttgagc agccacgtga
     3661 atgcggtgta tgcgacagcg gaactcatca cggccaggta cagcaacgcg agcatgccgg
     3721 tcggttccca gtgccattgc gggagttcgc cgcgcgccag cccgcccacc agcaggatca
     3781 cactgcccag cagcatgatc gcggcgttga acgcgatcgg ccccagaccc agcagcacgt
     3841 tgcgatactg caccgtgccg atcgcccacg acaggcagcc gagcaacacc acgatctgcc
     3901 acccgattcg cccggtctcg agcagattct cgggccagac gagcagcgcg acgccgccaa
     3961 atccgagcag catgccgatg ccgctgggca gcgagatttt ctgggcct
//
LOCUS       DASPFR010000325        10310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_948509, whole genome shotgun sequence.
ACCESSION   DASPFR010000325 DASPFR010000000
VERSION     DASPFR010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10310
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948509"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2125
                     /gene="malQ"
                     /locus_tag="VD737_08590"
     CDS             <1..2125
                     /gene="malQ"
                     /locus_tag="VD737_08590"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HXW10658.1"
                     /translation="RGGLNWFSRETKAAILASMGCDVADEAGVRRELDKVESQRWATL
                     LPPVAIVRQAPRTVPVNVPADALEQRLAWRMVLADGTRRDGEARAADLDEAERHEVGG
                     RLWTRRRLPVDEHLPPGRHELRAALGTGPEGSCALIVAPPRCFEPESLQAGERLWGVA
                     VQLYTLRSRENWGIGDFADLEYVVRRAALHGASFVGLNPLHALFPANPAHFSPYSPSS
                     RHFLNVLYVAVPRIPEYAECAEAVAHASTAEFRAEVARLRATGVVDYRGVTQLKLALL
                     RKVYRQFVRHHLAGRLTPRAEAFRRYRDERGDALRLHALHDAISEHLRGQDADRFWGW
                     PAWPEELREPSRAGVAEFARQHVDDVQFHAWLQWIADEQLGAVQRLAAELGMPIGLYG
                     DYAVGVNPAGSETWSDQRVYRMGAAVGAPPDPLALKGQDWGIPPQDPQALVAARYEPF
                     RTLIAANMRHFGALRIDHVMALFRQWWVPVGFGSTKGGYVHYPLDDLMSVVALESVRH
                     RCLVVGEDLGTVPDEIRRAMPEYAIYHYKVLLFEKVEGGRFREPGHFERRAIATVTTH
                     DLPTLRGYWEGHDLALRDALDLFPGEEIRRQVYDDRRRDRAALLEALEATGLRPRDPA
                     PGTETPFSDDLARAIQLYLARSSAALVVLQVEDLIGMTDPVNVPGTSDEHPNWQRKTT
                     ATIEEVFEDEATAELLEDVQFARCS"
     gene            2150..3385
                     /locus_tag="VD737_08595"
     CDS             2150..3385
                     /locus_tag="VD737_08595"
                     /inference="COORDINATES: protein motif:HMM:NF024622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HXW10659.1"
                     /translation="MRFRLASPEDYPAGLNLPWATPLEEWPVALFVDVERGLHRNLVS
                     FVEHDGTLYAIKELLEHVAAREYALLRSLRQLDLPVVEPVGLVTDRPVTRGRDRRGLL
                     VTRFLRHSMPYRHVIASGTTEAHAGQLLDALAELLVNLHLAGFYWGDCSLSNALYRLD
                     AGRYSAYLVDAETGELHPTLSSGQRAHDLQIAEENVAGEFMDIAAGFGLPANLDPASL
                     AEGLVARYERLWVELTREEVFAPHEQYRVDARVARLNDLGFDVADLDLRSIEGGQQVR
                     LRVQVVEPGHHRHRLEALTGLDVQENQARRLLNDLEHHRAVWSEAEKRRIDDREAASR
                     WLGQVYRPAVDSIPATQRGKLDDAEIFHQLLEHRWYLSERERRDVPMEEVIVAYARDV
                     LASLPDSPVRQVAVTTRET"
     gene            3387..3725
                     /locus_tag="VD737_08600"
     CDS             3387..3725
                     /locus_tag="VD737_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10660.1"
                     /translation="MKAVPMRGKLIGKRISGYMWAGTGNSWMRKENYFQLEDHRLVTA
                     IFGTPSGSLEIRCRGATFRLLLKGQEGQVLSTAVLALPGGRKAVAVQPIEEIGRGSKR
                     RRKRKVARKR"
     gene            3738..4196
                     /locus_tag="VD737_08605"
     CDS             3738..4196
                     /locus_tag="VD737_08605"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXW10661.1"
                     /translation="MIKIVGTTRILERHRATCHCGAVELELSLPDGIVDPRRCDCSIC
                     RRKGAIVASVPLSGLRVVRGQDALALYRFNTQTAQHFFCSNCGIYTHHRRRSNPDQYG
                     YNVGCLEGVNPFDLGHVPTSDGVNHPADRREAATKREGDPGNSVTTREQT"
     gene            4249..4653
                     /locus_tag="VD737_08610"
     CDS             4249..4653
                     /locus_tag="VD737_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10662.1"
                     /translation="MMAATVGVVLAIAVGALGTMAGLERDRAYYPVVTIVVASYYALF
                     AAMAGSAQALVLESLAGALFAAVAVAGFRRSLWLTAGALAAHGFFDLGHADLVANPGV
                     PGWWPAFCLTFDVAAAAWLAWRLRTGRIRAAA"
     gene            4696..5202
                     /locus_tag="VD737_08615"
     CDS             4696..5202
                     /locus_tag="VD737_08615"
                     /inference="COORDINATES: protein motif:HMM:NF037472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HXW10663.1"
                     /translation="MNSRRPPPRRTSTLRRLGTTVLPLVLGLVSVDTAFADETSPPAK
                     EWRSYCQSYMKALEGDADASDLDVTYCLGVTKGLLSGLRVGAQLGALSFGSRLAVQHQ
                     LDADAIFSQFQQQDPSRLLGVCAPATATTPDFVRPVLAQLEKNPGDAERPIAEVFYEA
                     LSAAYPCR"
     gene            complement(5266..5883)
                     /locus_tag="VD737_08620"
     CDS             complement(5266..5883)
                     /locus_tag="VD737_08620"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HXW10664.1"
                     /translation="MTVSTKLLRKAGLALVVALPCAVLAAPPDWAPAHGWRKKNDPSY
                     AGYRGYSGKDWDSDYGVKSGSCDRGRVGQVVGGVVGGVAGGAIGAEVAKGSENRTVAI
                     VVGTVIGAAIGSEVGRRMDKTDRSCVGHSLELVAANQNVKWTNPNTRVTYQLTPLDER
                     PRGDGCRSFRLIAHGAFGLSEGRTMACPDGAGVWRLAEDRRVSQR"
     gene            6114..8036
                     /locus_tag="VD737_08625"
     CDS             6114..8036
                     /locus_tag="VD737_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXW10665.1"
                     /translation="MSETFGNGRRYRPWLLIVSVYLVIGLLTRLVLWARFGIDADVTP
                     GHVPWIVGAGAVNDVIEALYLFAPLALYLGLMPDRWFSSAANRALLPVVCACVIAGLL
                     YLAAAEFFFFEEFDARFNIVAFDYLAYPTEVFTDIREAYPVGEVLLASLLCGAAAAYA
                     SRGLLRASTAASSRLRDRLAFLATYAVAVGLAVGFYPTDTLSWSPNRVENELVQNGSS
                     SFFRAARTRDIDYADWYLTGSRRQNLDELQAQLGADGSRFTRLREGRLDRRHAARPDG
                     LGPLNVVVIVSESFGAEFSRLHGARRDWTPNFDAYARRGLWFSNAYASGTRTVRGLEA
                     ITASFPPIPTVSILRRPGSEHIATWGSVMRRLGYRTSFLYGGYAYFDDMNRFYAGNGY
                     EVLDRANIDAPRYANVWGVSDEDLFDLALTHFGRQAATGEPFFSIVMTTSNHKPFSFR
                     TGLEPLGIPPQGGGRKAGVRYADYALGYFLRRAARQPWFDDTLFVVVADHGARVYGKA
                     EIPLETYEIPLMMYSPKHLAPRSVDVLTTQIDVAPTVLGLLGLPYEAPFFGVDVLHQQ
                     PGTARVALFSHNHDVALLRDDRLAVLELGKKTESLRYDRAAGAFVRQPDDAQLVPLAV
                     AYFQTAAELFRAHQYD"
     gene            complement(8077..8700)
                     /locus_tag="VD737_08630"
     CDS             complement(8077..8700)
                     /locus_tag="VD737_08630"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXW10666.1"
                     /translation="MHKPSSVVGLALLLALAVATTHAAPGVETRADREAVAELMYCYA
                     RGTDEIGDATTHPDPLAAGLAVYQGCFAPDAEFRAWFPQQPFDSQTFPNPDAHPDTAP
                     APFVGPAAWAGFVNAVFRGNGYTFTQHMITNVDVQLQGNRALLTAYLNATHVISGEAI
                     GGPSRCVAVANGTYSMQAVKRRGRWVATGLDLTLITFNPIFQTGAGC"
     gene            complement(8811..>10310)
                     /locus_tag="VD737_08635"
     CDS             complement(8811..>10310)
                     /locus_tag="VD737_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005264012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HXW10667.1"
                     /translation="RTASRPGPERPGGVGTRAASTSDVAPESILRFHRRPPKNADRER
                     SVIRMPSYERVAADPILYAHASRILHQEANPGNARALVQRHGDADVWLNPPPIPLTTR
                     EMDAVYELPYQRVPHPSYGDAKIPAYEMIRFSVAIQRGCFGGCTFCSITEHEGRIIQN
                     RSEDSVIREIETIRDTVPGFTGVISDLGGPTANMYRVACKSREIEAACRKPSCVYPGI
                     CPNLNSDHSPLIKLYRRARELKGVKKVLIASGVRYDLATESPEYVKELAQHHVGGYLK
                     IAPEAIGEGPLSKMMKPGVGTYDRFKLLFDKYSKEAGKEQYLIPYFIAAHPGTRDEDM
                     LELAIWLKRNGFRADQVQAFLPSPMATATAMYHSGKNPLHKVTRTSEDVVVPKGTRQR
                     RLHKAFLRYHDPNNWPLLREALRQMGRADLIGNGRHQLVPTYQPIGTGQRHEGKRKAI
                     KAGPAPTPSTPPAKRDPPHQPFRTQHTGLPRVPAAPRAGRRPAARRHPR"
BASE COUNT         1486 a         3544 c         3618 g         1662 t
ORIGIN      
        1 ccgtggcggg ctgaactggt tcagccgcga gaccaaggct gcgatcctcg cgtccatggg
       61 atgcgacgtt gccgacgagg cgggcgtgcg ccgcgaactc gacaaggtcg aatcgcagcg
      121 ctgggcgacg ttgttgccac cggtcgcgat cgtgcggcag gcgccgcgca cggtgccggt
      181 caacgtgccg gccgacgcgc tcgagcagcg gctggcgtgg cgcatggtcc tcgcggacgg
      241 cacgcgacgc gacggcgagg cgcgcgccgc ggacctcgac gaagccgagc gccacgaggt
      301 cggtgggcgg ctgtggacac gtcgacggct gccggtcgac gagcacctgc cgccgggccg
      361 ccacgagctg cgggcggcgc tcggtaccgg ccccgagggc tcgtgtgccc tgatcgtggc
      421 gccgccgcgg tgcttcgagc ccgagtccct gcaggccggc gagcggctct ggggcgtcgc
      481 ggtgcagctg tacacgctgc gctcgcgcga gaactggggc atcggcgact tcgccgacct
      541 ggagtacgtc gtgcggcgcg cggcgctgca cggtgcctcg ttcgtcggcc tcaacccgct
      601 gcacgcgctc tttcccgcga acccggcgca cttcagcccg tacagcccgt cgtcgcggca
      661 cttcctgaac gtgctgtacg tcgccgtgcc gcggatcccc gagtacgccg aatgcgccga
      721 ggcggtcgcg cacgcctcga cggcggagtt ccgcgccgaa gtcgcgcgcc tgcgcgcgac
      781 cggcgtcgtc gactatcgcg gcgtcacgca gctcaagctc gcgctgctgc gcaaggtgta
      841 ccggcaattc gtacggcacc acctggcggg ccgcctgacg ccccgcgccg aggcgttccg
      901 gcgatatcgt gacgagcgcg gcgatgcgct gcggctgcac gccctgcacg atgcgatctc
      961 ggagcacctg cggggccagg acgccgatcg gttctgggga tggcccgcgt ggcccgagga
     1021 gctgcgcgag ccgtcgcgcg cgggcgtcgc cgagtttgcg cgccagcacg tcgacgacgt
     1081 gcagttccac gcgtggctgc agtggattgc cgacgagcag ctgggcgccg tgcagcggct
     1141 cgccgccgag ctcggcatgc cgatcgggct ctacggcgac tacgccgtgg gcgtgaaccc
     1201 ggccgggtcg gagacctggt cggaccagcg cgtctaccgc atgggggccg ccgtgggggc
     1261 ccctcccgac ccgctggcgc tcaagggaca ggactggggc attccgccgc aggatcccca
     1321 ggcgctggtc gccgcgcgat acgagccgtt ccgcacgctg atcgccgcga acatgcggca
     1381 tttcggcgcg cttcgcatcg accacgtgat ggcgctgttc cgccagtggt gggtgccggt
     1441 cgggttcggg tcgacgaagg gcggctacgt gcactacccg ctcgacgacc tgatgtcggt
     1501 ggtcgcgctc gaaagcgtcc ggcaccgttg cctcgtggtc ggcgaagacc tcggcaccgt
     1561 cccagacgag attcgccggg ccatgcccga gtacgcgatc tatcactaca aggtgctgct
     1621 gttcgaaaag gtcgaaggcg ggcgcttccg cgaacccggg cacttcgagc gtcgcgcgat
     1681 cgcgacggtg acgacgcacg acctgcccac gctgcgcggc tactgggaag gccacgacct
     1741 cgcgttgcgc gacgcgctcg atctcttccc cggcgaggag attcgccggc aggtgtacga
     1801 cgaccgcagg cgcgaccgcg ccgcgctgct cgaagcgctc gaggcgaccg ggctgcgacc
     1861 gcgcgacccc gcccccggca cggagacgcc gttttcggac gacctggccc gcgcaatcca
     1921 gttgtacctc gcgcgcagtt cggccgcgct cgtcgtgctg caggtcgagg acctgatcgg
     1981 catgaccgac ccggtcaacg tgccaggcac gagcgacgag cacccgaact ggcagcgcaa
     2041 gacgacggcc acgatcgagg aggtgttcga ggacgaggcc acggcagagt tgctcgagga
     2101 cgtgcagttc gcacggtgct cgtgagtccg tgggtcgggg gcgcaagcga tgcggttccg
     2161 gctggcgtca cctgaagact acccggccgg cctcaacctg ccgtgggcca cgcccctcga
     2221 ggagtggccg gtggcgttgt tcgtcgacgt cgaacgcggc ctgcatcgca acctggtgag
     2281 cttcgtcgag cacgacggca ccctctacgc gatcaaggaa ctgctcgagc acgtcgcggc
     2341 gcgcgagtac gccctgctgc ggtcgttgcg gcagctcgac ctgcctgtcg tcgaaccggt
     2401 cgggctggtc acggaccgtc ccgtcacgcg cggccgggac cgccgggggt tgctggtcac
     2461 ccgcttcctg cggcattcga tgccgtaccg gcacgtgatc gcgagcggca cgacggaagc
     2521 acacgccggt cagttgctcg acgcgctggc cgagctgctc gtgaaccttc atctggcggg
     2581 gttctactgg ggcgactgtt cactgtcgaa cgcgttgtac aggctggacg ccggccgcta
     2641 cagcgcctac ctcgtcgacg cggagaccgg tgagctccat ccgacgctct cctccgggca
     2701 gcgtgcgcac gatctgcaga tcgccgagga gaacgtcgcg ggcgagttca tggacatcgc
     2761 ggcggggttc gggctgcccg cgaacctcga tccggcgtcg ctcgccgagg ggctcgtcgc
     2821 gcgctacgag cggctgtggg tcgagctgac gcgcgaggag gtgttcgcac cgcacgagca
     2881 atatcgtgtc gacgcgcgcg tcgcccggct gaacgacctc ggcttcgacg tcgcggacct
     2941 cgacctgcgc agcatcgagg gcggccagca ggtgcggttg cgcgtgcagg tcgtcgagcc
     3001 cggccatcac cgccaccgtc tcgaggcgct gaccggcctc gacgtgcagg agaaccaggc
     3061 gcgtcgcctg ctgaacgacc tcgagcacca ccgcgcggtg tggtcggaag cggagaagcg
     3121 ccgcatcgac gaccgcgagg ccgccagccg gtggctcggc caggtgtatc gcccggccgt
     3181 ggactcgata ccggccacgc agcgcggcaa gctcgacgat gcggagatct tccaccagct
     3241 gctggagcac cgctggtacc tctccgagcg cgagcggcgc gacgtgccga tggaggaagt
     3301 gatcgtggcc tacgccaggg acgtcctggc gtcgctgccc gattcgcccg ttcgtcaggt
     3361 ggccgtgacc accagggaga cgtgatgtga aagccgttcc aatgcgggga aagctgatcg
     3421 gcaagcggat ctcgggctat atgtgggctg gcaccggcaa cagctggatg cgcaaggaga
     3481 actacttcca gctcgaggat cacaggctcg tcaccgcgat cttcggcacg ccctccgggt
     3541 cgctcgagat ccgttgccgc ggcgcgacgt ttcgactgtt gctgaagggg caggaggggc
     3601 aggtgctgtc gaccgccgtg ctcgcgctgc cgggcgggcg caaggccgtg gcagtgcagc
     3661 cgatcgagga gatcggccgc ggcagcaagc gacgcaggaa gcgcaaggtt gcgcgcaaac
     3721 gttgaccggg caaccgcatg atcaagatcg tcggcacgac ccgcatcctc gaacgacacc
     3781 gggcgacctg ccattgcggt gccgtcgagc tcgagctgtc gctgcccgac ggcatcgtcg
     3841 atccccgtcg ctgcgactgc tcgatatgca ggcgcaaggg cgccatcgtg gcatcggtgc
     3901 cgttgtccgg gctgcgcgtg gtgagaggcc aggacgcgct ggcgctgtat cgattcaaca
     3961 cccagaccgc ccagcatttc ttctgctcga actgcggcat atacacgcac catcggcgcc
     4021 gctcgaaccc ggatcagtac ggttacaacg tcggatgcct cgagggcgtc aacccgttcg
     4081 acctggggca cgtgccgacc agcgacggcg tgaatcaccc ggccgaccgg cgcgaagccg
     4141 caacgaagcg cgagggcgac ccggggaatt ccgtcacgac gagggagcag acatgaccgg
     4201 tgtggtcgtc gggttccgat acgcgtgggc acgctgggtg cgcttcgcat gatggctgca
     4261 accgtcggcg tcgtgcttgc gatcgcggtc ggcgcgctcg gcacgatggc gggtctcgag
     4321 cgcgatcgcg cctactaccc cgtcgtgacg atcgtggtgg cgtcgtacta cgcgctgttc
     4381 gccgccatgg cgggttccgc gcaggcgctg gtgctcgagt cgctggccgg cgcgctgttc
     4441 gccgcggtgg ccgtcgcggg attccggcgc tcgctgtggc tgaccgcggg cgcactggcc
     4501 gcgcacgggt tcttcgatct cgggcacgcc gacctggtcg ccaatcccgg cgtgccgggc
     4561 tggtggccgg ccttctgcct caccttcgac gtcgcggccg cggcctggct ggcgtggcgg
     4621 cttcgcacgg gacgcatccg ggccgccgcc tgatcggcac acgtctcgcg gcgggcgcga
     4681 cggtatgatc ccggcatgaa ctctcgacga ccgcccccgc ggcgcacttc gactctcagg
     4741 cgactcggca cgaccgtgct cccgctggtg ctcgggctcg tttcggtcga caccgcattc
     4801 gccgacgaaa cctcgccgcc ggcgaaggaa tggcgcagct actgccagag ctacatgaag
     4861 gctctcgagg gggacgctga cgcgagcgat ctcgacgtca cgtactgcct gggcgtcacc
     4921 aagggcctgc tcagcggcct gcgcgtcggc gcccaactcg gcgcgctcag cttcggatcc
     4981 cgcctcgcgg tccagcacca gctcgacgcg gacgcgatct tctcgcagtt ccagcagcag
     5041 gatccgtcgc gactgctcgg cgtttgcgca ccggcgaccg cgacgacccc cgacttcgtg
     5101 cgaccggtgc tggcgcagct cgagaagaac cccggcgacg ccgagcggcc gatcgccgag
     5161 gtcttctacg aagccctgtc ggcggcatat ccctgccgct gagcacggcc ctcacccggc
     5221 gctacgcgcc gacctctccc accggtgcgg gagaggtagg gagcctcagc gctggctgac
     5281 gcgccgatcc tccgcgaggc gccacacgcc ggcgccgtcc gggcacgcca tggtgcggcc
     5341 ttcgctcaat ccgaaggcgc cgtgcgcaat gaggcggaag gagcgacagc cgtcgccgcg
     5401 cggacgctcg tcgagcggcg tcagctggta cgtgacgcgc gtgttcgggt tggtccactt
     5461 gacgttctgg ttcgcagcga ccagctcgag cgagtgaccc acgcacgagc ggtcggtctt
     5521 gtccatgcga cgacccacct ccgagccgat cgccgcgccg atgaccgtgc cgaccacgat
     5581 cgcgacggtg cggttctcgc tgcccttggc gacctcggca ccgatggcgc cgccggcgac
     5641 gccacccacc acgccgccga cgacctgacc gacgcggcca cgatcgcacg agccggactt
     5701 cacgccgtaa tccgagtccc agtccttgcc tgagtagccg cggtagccgg catagctcgg
     5761 gtcgttcttc ttgcgccaac cgtgtgcggg cgcccagtcg ggcggcgccg ccaggaccgc
     5821 acacggcagc gccaccacca gggcgagtcc cgccttgcgc aggagtttcg tggaaaccgt
     5881 catcgaaacc tccccggctt tcggccggtc gagcccatct gggcacgcac gaaccgtagc
     5941 ccctgggccc gagtggggcg cggcatcggc acggttcggt gacgaacgtc acggcgcccg
     6001 tcgcgattat gtaaagcgag cgcgggcggt ggatattgct ccgcccgggc gtccaacgag
     6061 ggcaggtcgg cggtccgcgg ggaccggccc tgcatccctc gttcggagtc tgcatgtcag
     6121 aaaccttcgg caacgggcgg cgctaccggc cctggctgct catcgtgtcc gtctacctgg
     6181 tgatcggcct gctgacccgg ctggtgctgt gggcacggtt cgggatcgat gccgacgtca
     6241 cgcccggtca cgtcccgtgg atcgtcgggg ccggggcggt caacgacgtg atcgaagcgc
     6301 tgtacctgtt tgcgccgctc gccctctacc tcggcctgat gccggaccga tggttcagtt
     6361 cggccgcgaa ccgcgcgttg ctgcccgtgg tctgcgcgtg cgtcatcgcc ggccttctgt
     6421 atctcgcggc cgctgaattc ttcttcttcg aggagttcga cgcccgcttc aacatcgtgg
     6481 ccttcgatta cctcgcgtat cccacggaag tgttcacgga catccgcgag gcctacccgg
     6541 tcggggaggt gctcctggcg tcgctgctct gcggcgcggc cgcggcgtat gcatcgcgcg
     6601 gcctgctgcg agccagcacg gcagcgtcct cgcgcctccg cgacaggctc gccttcctgg
     6661 ccacctatgc ggtggccgtc ggactcgcgg tcgggttcta cccgaccgac acgctgtcgt
     6721 ggtcgccgaa ccgcgtcgag aacgaactcg tgcagaacgg ctccagcagc ttcttccgcg
     6781 cggcgcgcac gcgcgacatc gactatgccg actggtacct gaccggttcg cggcggcaga
     6841 acctcgacga actccaggca cagctcggcg ccgacgggtc acggttcacg cgcctgcggg
     6901 agggccggct cgatcggcgc cacgccgccc gcccggacgg gctcggcccc ctcaacgtcg
     6961 tcgtgatcgt gagcgagtcg ttcggtgccg agttcagccg cctgcacggc gctcgacgcg
     7021 actggacgcc gaacttcgac gcctacgcac gacgaggact ctggttctcc aatgcctatg
     7081 cctcgggcac gaggaccgtg cgggggctcg aggcgatcac ggcctcgttt ccgccgatcc
     7141 cgaccgtgtc gatcctgcgc cgccccggca gcgagcacat cgcgacgtgg ggcagcgtca
     7201 tgcgtcggct cggctatcga accagcttcc tctatggtgg ctacgcgtat ttcgacgaca
     7261 tgaaccggtt ctacgccggg aacggctacg aggtcctcga ccgggcgaac atcgacgcgc
     7321 cccgctacgc gaacgtgtgg ggcgtgagcg acgaggacct cttcgacctg gcgctcacgc
     7381 acttcggacg gcaggccgcg accggcgagc cgttcttctc gatcgtcatg acgacgtcga
     7441 accacaagcc gttctcgttc cgtaccggcc tcgagccact cggcattccg ccgcagggag
     7501 gtggccgcaa ggccggtgtc cgctacgccg actacgcgct cggctacttc ctgcgccggg
     7561 cagcccggca gccctggttc gacgacacgc tcttcgtcgt cgtcgcggac cacggcgcgc
     7621 gcgtctatgg caaggccgaa atcccgctcg aaacctacga gatcccgctg atgatgtact
     7681 cgccgaagca cctcgccccg cgcagcgtgg acgtgctgac gacgcagatc gacgtcgcgc
     7741 cgacggtcct cggcctgctc gggctgccgt acgaagcgcc gttcttcggc gtcgacgtgc
     7801 tgcaccagca acccggcacc gcgcgcgtcg cgctcttcag tcacaaccac gacgtggcgc
     7861 tgctgcgcga cgatcgattg gcggtgctgg agctcggcaa gaaaacggag tcgctccgat
     7921 acgaccgtgc cgcgggggcg ttcgtgcggc aaccggacga cgcgcagctg gtgccgctcg
     7981 ccgtggccta tttccagacg gccgcggagc tgttcagggc ccaccagtac gattgacgcc
     8041 gcgcggtctg ccccgtcacg gcgtcaccgg acttcttcaa cagccggcgc ctgtctggaa
     8101 gatcggattg aacgtgatca aggtgagatc gagcccggtc gcaacccagc ggccgcgacg
     8161 cttcacggcc tgcatcgagt aggtgccgtt cgcgaccgcg acgcagcgcg acggcccgcc
     8221 gatcgcctcc cccgagatca cgtgtgtggc attgaggtag gccgtgagca gcgcacggtt
     8281 gccctgcagc tgcacgtcca cgttcgtgat catgtgctgg gtgaaggtat agccgttgcc
     8341 gcggaacacg gcattgacga acccggccca cgcggccggg cccacgaacg gcgccggtgc
     8401 ggtgtcagga tgcgcgtcgg gattcgggaa cgtctgcgaa tcgaagggct gctgcgggaa
     8461 ccacgcgcgg aactcggcgt ccggcgcgaa gcagccctgg tagaccgcga gcccggctgc
     8521 cagcgggtcc gggtgcgtcg tcgcatcgcc gatctcgtcc gtgccgcgcg cgtagcagta
     8581 catcagttca gccacggctt cgcgatccgc gcgagtctcg acgccggggg cggcgtgagt
     8641 cgtcgccacg gcaagggcca gcaagagcgc gaggccgacg acgctggatg gtttgtgcat
     8701 ggcgttctcc tgcggttgtg tgtgccgtgg acggcaagtc acgtgtgctt gcttgtttcc
     8761 aacaagtgta gcacctggct cgcggtgcgg tgagccgtgc ctggcggtcg ttaccgcggg
     8821 tggcggcgag ccgccggccg tcggcccgcc ctcggcgcgg ccgggacccg cggcagtccg
     8881 gtgtgctggg tgcgaaacgg ctggtgcggc gggtcccgct tcgccggggg cgtcgacggc
     8941 gtcggcgctg gtcctgcctt gatggccttg cgcttgcctt cgtggcgctg gccggtgccg
     9001 atcggctggt acgtaggcac gagctggtga cgcccgttgc cgatcaggtc cgcgcggccc
     9061 atctggcgca gcgcttcgcg cagcagcggc cagttgttgg gatcgtggta gcgcaggaag
     9121 gccttgtgca gacgacgctg gcgcgtgccc ttggggacca cgacgtcctc cgacgtacgc
     9181 gtgaccttgt gcagtggatt cttgcccgag tggtacatcg ccgtggccgt cgccatcggc
     9241 gagggcagga aggcctgcac ctggtcggcg cggaacccgt tgcgcttgag ccagatcgcc
     9301 agctcgagca tgtcctcgtc gcgcgtgccc ggatgcgccg cgatgaagta cgggatcagg
     9361 tattgctcct tccccgcttc cttcgagtac ttgtcgaaga gcagcttgaa gcggtcgtag
     9421 gtgccgacgc ccggcttcat catcttcgac agcgggcctt ccccgatcgc ctctggcgcg
     9481 atcttcaggt agccgccgac gtggtgctgt gcgagttcct tgacgtactc cggcgactcg
     9541 gtcgcgaggt cgtagcgcac gcccgaggcg atcagcactt tcttgacgcc cttcagttcg
     9601 cgcgcgcgcc gatacagctt gatgagcggc gagtggtcgc tgttgaggtt ggggcagatg
     9661 cccggataga cgcacgacgg cttgcgacac gcggcctcga tctcgcgcga cttgcaggcg
     9721 acgcgataca tgttcgccgt ggggccgccg aggtcggaga tcacgccggt gaagcccggc
     9781 acggtgtcgc ggatcgtctc gatctcgcgg atcacggaat cttccgagcg gttctggatg
     9841 atccggcctt cgtgctcggt gatcgagcag aacgtgcagc cgccgaagca gccgcgctgg
     9901 atcgcgaccg aaaagcggat catttcgtac gccggaatct tcgcgtcgcc gtacgaaggg
     9961 tgcggcacgc gctggtacgg cagctcgtat accgcgtcca tttccctggt cgtgagcgga
    10021 atcggcggcg ggttgagcca gacgtcggca tcaccgtggc gctgcaccag cgcgcgtgcg
    10081 ttcccgggat tcgcctcctg gtgaaggatg cgcgaggcgt gcgcgtacag gatggggtcc
    10141 gcggccactc gctcgtacga cggcatgcgg atgacgctgc gctcccggtc cgcgttcttc
    10201 ggcggcctgc ggtggaagcg gaggatggac tcgggtgcga cgtcggaagt ggatgcagcg
    10261 cgcgtaccga cgccgccagg gcgttccgga cctggccggg aggccgtccg
//
LOCUS       DASPFR010000326         7224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1559465, whole genome shotgun sequence.
ACCESSION   DASPFR010000326 DASPFR010000000
VERSION     DASPFR010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7224
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559465"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VD737_08640"
     CDS             complement(<1..228)
                     /locus_tag="VD737_08640"
                     /inference="COORDINATES: protein motif:HMM:NF016262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HXW10668.1"
                     /translation="MTASNDGTGGGGGDGTRLRGTGDGERPADAAEDTLFKFPTDFPV
                     KIMGQASDDFRSLVLGIVTRHFGPLEPHRIEE"
     gene            complement(225..1109)
                     /locus_tag="VD737_08645"
     CDS             complement(225..1109)
                     /locus_tag="VD737_08645"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HXW10669.1"
                     /translation="MADPFPTCWLDGAFVSLAEARVSPLDRGFLFADGVYEVVPVHRR
                     RPFRLRQHLERLERSLGELRMRNALPREAWLQVIDRLVADAGEPELLVYLQVTRGAQF
                     GRNHLFPPADVAPTGFAFVSPYPPPAPSTLQRGLAASLLEDTRWARCDVKSVALLANV
                     LLRQEAEDRGAAEAILYRGDRVTEGSSSTVFIVSDRRLATPPNGREILPGTTRDAVLE
                     LVADWMPCDVRGISVDELRGADEVWIASAGRGVLPVTSVDGAAIGAGTPGPAWRRTYE
                     ALQRHLDEMAKRPALEDA"
     gene            complement(1126..2352)
                     /locus_tag="VD737_08650"
     CDS             complement(1126..2352)
                     /locus_tag="VD737_08650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HXW10670.1"
                     /translation="MTFKHSLLALALAGCFGLALTGCGSDDAPANADAAADAPPAEAA
                     QAVAPAGPTAVPPPPELKARSFIVIDHDSGRVLAALEPDTRQEPASLTKLMTAYGVFK
                     ALKEGRLKPTDMVTISERAWKQEGSRMFVEVGKQVSVNDLLQGMIVQSGNDATVALAE
                     KVGGTEETFVQMMNTYAKQLGMTNSHFTNSPGMPGPEHYMTARDAATLASALIHEFPE
                     YYKLYAQREFAWNGITQQNRNGLLWRDPTVDGVKTGHTETAGYCLIASARRDGMRLVS
                     VVLGADSMRAREDASAALLNYGFNFFETRRIYAAGQPLTKTRVWKGKEDEVGLTLKRD
                     LYVTSQRGGVDSVKAEFELPETLEAPLSRNEAVGKTTILVDAAPVAVYDLYPAEDVAA
                     AGFFGRTWDSLRLMLH"
     gene            complement(2398..3474)
                     /gene="mltB"
                     /locus_tag="VD737_08655"
     CDS             complement(2398..3474)
                     /gene="mltB"
                     /locus_tag="VD737_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HXW10671.1"
                     /translation="MTRNRPLRRAAHRPSGFAIAPLALAVAAAGAIPAAACAQAFDPG
                     RPDVAQFIADVSQRHGFEPGPLAAAFARMESKPAILQAIARPAERTLNWTEYRARFLT
                     DPRIARGIALHDERRAELQHAEQSTGVPAPVLLAIIGVETIYGQNTGSYRVADALATL
                     AFDYAPRATFFRGELEQFLLMTREEQLDPLVPLGSYAGAMGIPQFMPTSFRKWAVDGD
                     DDGHRDLWNDWADVFASVGNYLKAHGWEPGRPVMVPADVANADLTGLELGKIGLPETV
                     GSLRKRGVRFDVDLPETTAAALVALPMAFGNEYRVGFANFHAITRYNRSHLYASAVAD
                     LAEAIGAGVTAAPAAAPPPASPAR"
     gene            complement(3471..4619)
                     /gene="rodA"
                     /locus_tag="VD737_08660"
     CDS             complement(3471..4619)
                     /gene="rodA"
                     /locus_tag="VD737_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HXW10672.1"
                     /translation="MRTEIAGPGYSSRTQRTLTLTGRLLDALHLDGPLLVSVLAVCAA
                     GLIVLFSAAGEDVHIALGQAARVGLGMVVLALVAQVPPRVMRIGAPFLYGVGVLLLVA
                     VAVVGDVAMGAQRWLDLGVVRFQPSELLKIAVPLCCAWYLHDRPLPPDFTTLVTVVLI
                     VLVPTLLIAEQPDLGTSLLVASGGGLVILLAGLQLRYILSLVVLLGAAIPIVWTFLLH
                     GYQKQRVLTFLNPQNDPLGAGYHTIQSQIAIGSGGLFGKGYMNGSQAQLEFLPERSTD
                     FIFAVIGEEWGLVGCFVLMMLYLLVIGRGLYIATTAQDTFSRLIAGSLTLTFSVYVFV
                     NSGMVAGLLPVVGVPLPLVSYGGTSLVTLMAGFGILMSVHSRRKLVST"
     gene            complement(4616..6556)
                     /gene="mrdA"
                     /locus_tag="VD737_08665"
     CDS             complement(4616..6556)
                     /gene="mrdA"
                     /locus_tag="VD737_08665"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXW10673.1"
                     /translation="MARQSRLKDHHAEQRLFARRVVAASMVIFVLLGALFARMFWLQV
                     VRHDYFSELSQGNRIRIDPIPPPRGLILDRRGEPLALNRPAYQLELTREQTPDLDDTL
                     ARLVDLGLIAPEDLERTRRLITARRPFDAVPIRLQLSEEELARFAVHRPDFPGVEIRP
                     RLTRYYPHAGVGVHAIGYVGAISEKDQERIDESKYAGTTLIGKLGVERNFEETLHGDT
                     GYQQLLVNAQGRRVDRVGIKAPELERREPVAGDDLILAIDARLQRVVEAALGTQRAAV
                     VALDPANGDVLAFVSTPTFDPNGFARGLTVAEYRALEDNLDRPLFDRALRGVYPPGST
                     IKPLVALAALEYGVAAPGNTRFCRGVWQFPGSSFRFRDWKKEGHGTVDMKRAISQSCD
                     TYFYGVSDQIGIDRLHDFLVRFGLGSQTGIDVLGERDGLVPSREWKKQAFKKKELQVW
                     FPGETVIAGIGQGYMLATPLQLAHVAATIATRGKRFAPRIVRAVRDSQTGEVRELPVR
                     ELPEVKVKDPTSWDVIIGGMVAVMSPGGTAYRSQAGAPYQMAGKTGTAQVFTVARNQK
                     YNEKEISERLRDHALFIAFAPADAPKLAVAVLVENGRSGSGTAAPIARKVFDAFFLPA
                     SPTPASATPTPAPGTAPAGDEE"
     gene            complement(6570..7055)
                     /gene="mreD"
                     /locus_tag="VD737_08670"
     CDS             complement(6570..7055)
                     /gene="mreD"
                     /locus_tag="VD737_08670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HXW10674.1"
                     /translation="MRQQSMLARQLLYWLSMAAGLVLAIVPLPEWLDPVRPDLALLAM
                     IYFILTSPRIAGLGYAWLSGLFLDVLRGMVLGQHALGFLLVGYVTHKLQLQMRMFPIL
                     QQAAIVLLLLGLYHFVVFWIDGLTGHGYTAWVRWLPIISGALLWPLVVAVGDSLTRRS
                     G"
BASE COUNT         1118 a         2465 c         2580 g         1061 t
ORIGIN      
        1 ctcctcgatc cggtgcggct cgagcggacc gaaatgacgc gtgacgatgc cgagcacgag
       61 gctgcggaaa tcgtccgaag cctgccccat gatcttgacg gggaagtcgg ttggaaactt
      121 gaacagcgtg tcttcggccg cgtcggccgg gcgctcacca tcgcccgtgc cgcgcagacg
      181 cgtgccgtct ccgccgcctc cgccagtgcc gtcgttgctt gcggtcacgc atcctccagg
      241 gcaggccttt tcgccatctc gtccaggtgt cgctgcagcg cctcgtatgt gcgccgccac
      301 gcgggcccgg gcgtgccggc gccgattgca gcaccgtcca ccgacgtgac gggcagcacg
      361 ccgcgacctg ccgatgcgat ccagacctcg tctgcaccgc ggagttcatc gacggaaatg
      421 ccacgcacgt cgcatggcat ccagtccgcc acgagttcca gcacggcatc gcgcgtcgtg
      481 cccggcagga tttcgcgacc gttcggcggc gtggcgagcc ggcggtcgga cacgatgaag
      541 accgtgctgg acgagccttc cgtcactcga tcgccgcggt acaggatcgc ctcggccgcg
      601 ccgcggtcct cggcttcctg gcgcagcagc acgttggcga gcagcgccac cgacttgacg
      661 tcgcagcgcg cccagcgcgt gtcctcgagc agcgacgccg cgagcccccg ttgcagcgtc
      721 gacggcgccg gcggcgggta ggggctcacg aaggcgaagc cggtcggggc gacgtcggcg
      781 ggcgggaaca ggtggttgcg cccgaattgc gcaccgcgcg tgacctgcag gtagacgagc
      841 agctcaggct cgccggcgtc ggcgaccagg cgatcgatga cctgaagcca tgcctcccgc
      901 ggcagcgcgt tgcgcatgcg cagttccccg aggctgcgct cgagccgctc caggtgctgc
      961 cgcagccgaa acgggcggcg gcggtggacc ggcacgactt cgtagacgcc atcggcgaac
     1021 aggaacccgc gatcgagcgg ggacacgcgc gcctcggcga gcgacacgaa cgcgccgtcg
     1081 agccagcagg tcggaaacgg atcggccatc gtccgacgtc cgggctcagt gcagcatgag
     1141 gcgcaggctg tcccacgtgc gcccgaagaa tccggcggca gcgacgtcct cggcagggta
     1201 gaggtcgtac acggcgaccg gcgcagcgtc gaccaggatc gtcgtcttgc cgaccgcctc
     1261 gttgcgcgac agcggcgcct cgagcgtctc gggcagctcg aactccgcct tgacgctgtc
     1321 cacgccgccg cgctggctcg tgacgtagag atcgcgcttg agcgtgagtc cgacctcgtc
     1381 ctccttgccc ttccagacgc gcgtcttcgt gagcggctgt cccgcggcat agatgcgccg
     1441 ggtctcgaag aagttgaacc cgtaattgag cagggcggcg ctcgcatcct cgcgtgcacg
     1501 catcgagtcg gcgccgagca ccacgctgac gagacgcatg ccgtcgcgcc tggcggaggc
     1561 gatcaggcag tagccggcgg tttcggtgtg gccggtcttg acgccgtcca ccgtcgggtc
     1621 gcgccacagc aggccgttgc ggttctgttg cgtgatgccg ttccacgcga attcgcgctg
     1681 cgcatatagc ttgtagtact cgggaaactc gtggatcagc gcgctcgcga gtgtcgcggc
     1741 gtcgcgggcc gtcatgtaat gctcgggacc cggcatgccc ggcgagttgg tgaagtgcga
     1801 gttggtcatg ccgagctgct tcgcgtaggt gttcatcatc tgcacgaacg tctcctcggt
     1861 gccgccgacc ttctcggcga gcgcgacggt ggcgtcgttg cccgactgga cgatcatgcc
     1921 ctgcagcagg tcgttgaccg acacctgctt gccgacctcg acgaacatgc gcgacccttc
     1981 ctgtttccag gcgcgctcgc tgatcgtgac catgtcggtg ggcttgagcc ggccttcctt
     2041 gagcgccttg aacacgccgt aggccgtcat cagcttggtc aggctcgccg gttcctgccg
     2101 cgtgtcgggc tccagcgccg cgagcacgcg cccgctgtca tggtcgatca cgatgaagct
     2161 gcgcgccttg agctccggcg gaggcgggac ggccgtgggt ccggccggcg ccacggcctg
     2221 cgcggcttcg gcgggcggcg cgtcggcggc cgcgtcggca ttcgcgggcg cgtcgtccga
     2281 accgcaaccc gtcagtgcga gcccgaagca gccggcgagc gccagcgcga gtagcgagtg
     2341 cttgaaggtc atttcagcga tgtgggtccg tgcagaaggt gggtgaagaa gccgaggtca
     2401 tcgcgcgggc gacgccggtg gaggtgcagc cgccggggcg gccgtcactc cggcaccgat
     2461 cgcctcggcg aggtcggcga cggcgctggc atagaggtgg ctgcggttat accgcgtaat
     2521 cgcatggaag ttcgcgaagc cgacgcggta ctcgttcccg aacgccatcg gcagcgcgac
     2581 cagcgcagcg gcggtcgtct ccgggaggtc gacgtcgaaa cggacgccgc gcttgcgcag
     2641 cgagccgacg gtctcgggca ggccgatctt gccgagctcg aggccggtga ggtcggcatt
     2701 ggcgacgtcc gcgggcacca tcaccggccg gccaggctcc cagccgtgcg ctttcagata
     2761 gttgccgacg ctcgcgaaca cgtcggccca gtcgttccag aggtcacggt ggccgtcgtc
     2821 gtcgccgtcg acggcccact tgcggaagct ggtcggcatg aactggggga tgcccattgc
     2881 cccggcgtac gagccgagcg gcacgagcgg atcgagctgc tcctcccgcg tcatcaacag
     2941 gaactgctcg agttcgccgc ggaagaacgt cgcgcgcggc gcgtagtcga acgccagcgt
     3001 cgcgagggca tcggcgacgc gataactgcc ggtgttctgc ccgtagatcg tctcgactcc
     3061 aatgatcgcg agcagcacgg gcgcgggaac gccggtcgac tgctcggcgt gttgcagctc
     3121 ggctcgacgt tcgtcgtgca gcgcgatgcc gcgcgcgatg cgcgggtcag tcaggaaacg
     3181 cgcgcggtac tcggtccagt tgagcgtgcg ctcggcgggg cgggcgatcg cctgcaggat
     3241 cgccggcttc gattccatgc gcgcgaacgc agcggcgagc gggcccggct cgaagccgtg
     3301 ccgctgcgag acgtccgcga tgaactgcgc gacgtcgggg cgtccgggat cgaacgcctg
     3361 cgcgcatgcg gccgcgggaa tggcgccggc cgccgcgacg gcgagtgcga gcggggcaat
     3421 ggcgaagccc gacgggcgat gggcagcccg tcgcagggga cgattccgtg tcacgtcgag
     3481 accagtttgc ggcgggagtg gactgacatg aggataccga accccgccat gagggtcacg
     3541 agcgacgtgc cgccatagct caccagcggc agcggcacgc cgacgacggg cagcagcccc
     3601 gcgaccatgc cactgttgac gaatacgtac acggaaaacg tcagcgtgag gctgcccgcg
     3661 atcagccgcg agaacgtgtc ctgcgccgtc gtcgcgatgt agaggccgcg cccgatcacg
     3721 agcaggtaca gcatcatcag cacgaagcag ccgacgagcc cccattcctc gccgatgacc
     3781 gcgaagatga agtcggtcga gcgctcgggc aggaactcga gctgggcctg gctgccgttc
     3841 atgtaaccct tgccgaacag cccgccggaa ccgatcgcga tctgcgactg gatcgtgtgg
     3901 tatccggccc ccagcgggtc gttctgcggg ttgaggaacg tgagcacgcg ctgtttctgg
     3961 tagccatgca ggaggaacgt ccagacgatc gggattgccg cacccagcag gacgacgagg
     4021 ctcaggatgt aacgcagctg aaggcccgcg agcagaatca ccagccctcc gcccgacgcc
     4081 accagcaacg acgtgccgag gtcgggctgt tcggcgatca acagcgtcgg caccagcacg
     4141 atcagcacga cggtgacgag cgtcgtgaaa tcgggcggca gcggccggtc gtgcagatac
     4201 cacgcgcagc agagcggcac ggcgatcttg agcagctcgg acggctggaa ccgcaccacc
     4261 cccaggtcga gccaccgctg cgcgcccatg gcgacgtcgc cgacgacggc cacggcgacg
     4321 agcagcagca cgcccacccc gtacaggaac ggcgcgccga tgcgcatcac gcgcggcggt
     4381 acctgcgcca ccagcgccag gaccaccatg ccgaggccga cccgcgccgc ctgcccgagc
     4441 gcgatgtgca cgtcctcgcc cgcggcgctg aacagcacga tcagaccggc cgcgcagacc
     4501 gcgagcacgc tcaccagcag cgggccgtcc aggtgcagcg cgtcgagcag ccggcccgtc
     4561 agcgtcaggg tgcgctgcgt gcgactggaa tagccgggcc cggcgatctc ggttctcatt
     4621 cctcgtctcc cgcgggcgcc gttccgggcg caggcgtcgg tgttgcggac gccggcgtcg
     4681 ggctggccgg caggaagaac gcgtcgaaca ccttgcgcgc gatcggtgcg gccgtcccgc
     4741 tgccgctgcg gccgttctcg accagcaccg caaccgcgag cttcggcgcg tcggccggtg
     4801 cgaaggcaat gaagagcgcg tggtctcgca ggcgttcaga gatctccttc tcgttgtact
     4861 tctggttgcg cgccaccgtg aacacctgcg cggtgcccgt cttgccggcc atttggtagg
     4921 gcgcgcccgc ctgggaccga tacgccgtgc cgccgggact catcaccgcg accatgccgc
     4981 cgatgatgac gtcccacgac gtcgggtcct tgaccttgac ctcgggcagt tcgcggacgg
     5041 gcagctcgcg cacctcgccc gtctgcgagt cgcgcaccgc gcggacgatg cgcggcgcaa
     5101 accgcttgcc gcgcgtcgcg atcgtcgccg cgacgtgggc gagctgcagc ggcgtcgcaa
     5161 gcatgtaccc ctggccgatg ccggcgatga cggtctcgcc ggggaaccac acctgcagtt
     5221 ccttcttctt gaaggcctgc ttcttccact cgcgcgacgg caccaggccg tcgcgttcgc
     5281 cgagcacgtc gatgcctgtc tggctgccga ggccgaaccg cacgaggaaa tcgtggaggc
     5341 ggtcgatgcc gatctggtcg gacacgccgt agaaatacgt gtcgcacgac tgcgagatcg
     5401 cccgcttcat gtcgaccgtg ccgtggcctt ccttcttcca gtcgcggaaa cggaagctcg
     5461 acccggggaa ctgccagacg ccgcggcaga agcgcgtgtt gccgggcgcg gcgacgccgt
     5521 actcgagcgc ggcgagcgcc accagtggct tgatcgtcga tccgggcggg tacacgccgc
     5581 ggagcgcgcg gtcgaacagc ggccgatcga ggttgtcctc gagcgcccgg tactcggcga
     5641 cggtgaggcc gcgcgcgaag ccgttgggat cgaacgtcgg cgtgctcacg aacgcgagca
     5701 cgtcgccgtt cgcggggtcg agcgccacca ccgccgcgcg ctgcgtgccg agcgcggcct
     5761 cgaccacccg ctgcagccgc gcgtcgatcg cgaggatcag gtcgtcgccg gcgaccggct
     5821 cgcgacgctc gagctcgggt gccttgatgc ccacgcggtc gacgcggcgc ccctgcgcat
     5881 tgacgagcag ctgctggtag cccgtgtcgc cgtgcagcgt ctcctcgaag ttgcgctcga
     5941 cgccgagctt gccgatcagc gtagtgccgg cgtatttcga ctcgtcgatg cgctcctggt
     6001 ccttttcgct gatcgcgccg acgtacccga tcgcgtgcac gccgacgccg gcgtgtgggt
     6061 agtagcgcgt cagccgcggg cggatctcga cgcccgggaa gtccgggcgg tgcaccgcga
     6121 atcgcgccag ctcctcctcc gagagctgca gccggatcgg taccgcgtcg aacggccggc
     6181 gcgcggtgat caggcgccgc gtgcgttcga ggtcctccgg cgcgatcagg ccgagatcga
     6241 cgagtcgcgc gagcgtgtcg tcgaggtccg gcgtctgctc acgcgtgagc tcgagctggt
     6301 aggcaggccg gttgagggcg agcggctcgc cgcgccggtc gaggatcagc ccgcgcgggg
     6361 gcgggatcgg gtcgatgcgg atccggttgc cttgcgagag ttcgctgaaa tagtcgtgtc
     6421 gcaccacctg cagccagaac atccgcgcga acagcgcgcc gagcagcacg aagatgacca
     6481 tcgacgccgc gacgacccgc cgcgcgaaga ggcgctgttc ggcatggtgg tccttgaggc
     6541 gggactggcg tgccatggct cgcgcgtgct cagcccgagc gacgcgtgag cgagtcgccc
     6601 acggcgacga cgagcggcca gagcagtgcg cccgagatga tcggcagcca gcggacccag
     6661 gccgtgtagc cgtggccggt caagccgtcg atccagaaca cgacgaagtg atagagcccc
     6721 agcagcagca gcacgatggc ggcctgctgc aggatcggga acatgcgcat ctgcagttgc
     6781 agcttgtgcg tgacgtagcc gaccagcagg aagccgagcg cgtgctggcc gagcaccatg
     6841 ccgcgcagca cgtcgaggaa cagcccggac agccatgcat agccgagccc tgcgatgcgc
     6901 gggctcgtca ggatgaagta gatcatggcg agcagcgcga gatcggggcg caccgggtcg
     6961 agccactcgg gcaggggcac gatcgcgagc accagcccgg cggccatgct gagccagtag
     7021 agcagttgcc gcgccagcat gctctgctgc cgcatcactc gccggcctct tcgacaggcg
     7081 gcgcaggcgc aactggcgcg ggtgcgggcg acgtcgccgg cgtgacgggc cgcgtccccg
     7141 gtgcggggcg cgtgacgccg gcgggcgtgc tcggcgcgct cgcagtggcg gcaggcgccg
     7201 tcgtggcgcc gggcggggag cggg
//
LOCUS       DASPFR010000327         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1805070, whole genome shotgun sequence.
ACCESSION   DASPFR010000327 DASPFR010000000
VERSION     DASPFR010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1805070"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VD737_08675"
     CDS             complement(<1..721)
                     /locus_tag="VD737_08675"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HXW10675.1"
                     /translation="MQTDAPARAPEHAPINWVTSIVMIATPLAAITVVPWYGFTHGYS
                     LTAWLIALACLWAGGISITAGYHRLWAHRAYGAHWSVRLFFMLFGAMSLQNSILTWAS
                     QHRTHHRFVDDWDQDPYSAKRGFWFSHMGWILRNYPSGRDDFTNAKDLERDPLVVFQH
                     RYYLPLALFMNIGFPLLLGWAVGEVWGVFLLAGLLRLVVNHHFTWFINSLAHMWGSQP
                     YTDENTARDNPWLAFLTYGEGY"
     gene            997..2001
                     /locus_tag="VD737_08680"
     CDS             997..2001
                     /locus_tag="VD737_08680"
                     /inference="COORDINATES: protein motif:HMM:NF022664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3011 domain-containing protein"
                     /protein_id="HXW10676.1"
                     /translation="MIRSIARPVLAGLALLAAIPVASAQEASVLSAVGKARPKVQQPE
                     LKNAPIAVRSAQGDCVREANRRGFAVLDTSNFRQASEGWSLDMQVRDVRGRTSRGSCF
                     VETRTGDVSLYGFGWGYDDEGDDRLEFSCASTDKRYRECQLPIKGRARLVKRHSDSPC
                     TENQSWGQRGDRVWVDRGCRAKFEVVRASGGGGGANTFDCRSENGRYRECEIGPGYFG
                     RLVREYSNNRCRQDQTWGTRNGVVWVTDGCKAQFVRQRGNSGGGGGGGNDGDRQVECR
                     SRDGRYQECNIGRGFVGRLVQDESGGRCRRDSTWGTRDGVIWVTNGCSGRFERLRTKY
                     "
BASE COUNT          362 a          684 c          742 g          325 t
ORIGIN      
        1 gatagccctc gccgtacgtg aggaacgcga gccacggatt gtcgcgcgcg gtgttctcgt
       61 cggtgtacgg ctgcgagccc cacatgtgcg cgagcgagtt gatgaaccag gtgaagtgat
      121 ggttcacgac gagtcgcaac aggcccgcga gcaggaacac gccccagacc tcgcccacgg
      181 cccagccgag cagcagcggg aagccgatgt tcatgaacag cgcgagcggc aggtagtaac
      241 ggtgctggaa cacgaccagc ggatcgcgct cgaggtcctt cgcgttcgtg aagtcgtcgc
      301 gaccgctggg gtagttacgc aggatccagc ccatgtgcga gaaccagaat ccgcgcttcg
      361 cgctgtaggg gtcctggtcc cagtcgtcga cgaagcggtg gtgcgtgcgg tgctgcgacg
      421 cccaggtcag gatgctgttc tgcagcgaca tcgcgccgaa caacatgaag aacaaccgca
      481 ccgaccagtg cgcgccgtag gcgcggtgag cccacaggcg gtgatatcca gcggtgatcg
      541 aaatgccgcc ggcccagagg caggcgagcg cgatcagcca ggcggtcagg ctgtaaccat
      601 gcgtgaagcc gtaccagggc acgacggtga tcgcggccag cggcgtcgcg atcatcacga
      661 tgctggtcac ccagttgatc ggggcgtgct cgggggctcg ggcgggcgcg tcggtttgca
      721 tcagttttcc gtggggaaag gcggacagaa agtctcccct agtttaccgc ggcgccccgt
      781 gcccggatgg gggtttttgg gcagtgaatc cagatccgcg gcttttctgc cctggtcagg
      841 cgcgccggac tgcaactcgc cttgacgcag cgcatcattc cacgcctccg acatcctgcc
      901 gtcccgaccg ccggttgcca tcgaggcgcg cattggtcaa cgctgcgcgg gcggtcacgt
      961 tggatcgctg aaggcaacac gcctgcggag gcctccatga ttcgatcgat cgcccggccc
     1021 gtcctcgcgg gcctggcact gctcgccgcc atccccgtcg cctccgccca ggaagcgtcc
     1081 gtgctgagcg ccgtcggcaa ggcccggccc aaggtccagc agcctgaact caagaacgcg
     1141 ccgatcgcgg tgcgttcggc gcagggcgac tgcgtcaggg aagcaaaccg gcgcggtttt
     1201 gcggtcctcg acacgagcaa cttccggcag gccagcgagg gctggtcgct cgacatgcag
     1261 gtccgagacg tgcgcgggcg gacctcgcgc ggctcgtgct tcgtcgagac gcgcacgggc
     1321 gacgtgagcc tctacggctt cggctggggt tacgacgacg aaggcgacga ccgcctcgag
     1381 ttctcctgcg cctcgaccga caagcgctac cgcgaatgcc agctgccgat caagggacgg
     1441 gcacggctgg tcaagcgtca ctcggactcg ccgtgcaccg agaaccagag ctgggggcag
     1501 cgcggcgatc gcgtttgggt cgaccggggt tgccgcgcca agttcgaggt cgtccgcgct
     1561 tcggggggtg gcggcggcgc caacaccttc gactgccgtt ccgaaaacgg ccgataccgc
     1621 gaatgcgaga tcggccccgg atatttcgga cggctggtcc gggaatacag caacaaccga
     1681 tgtcgacagg accagacctg gggcacccgc aacggagtcg tctgggtcac cgatggctgc
     1741 aaggcgcagt tcgtgcgtca gcgcggcaat tccggcggcg gtggtggtgg cggcaacgac
     1801 ggagaccgac aggtggaatg tcgttcgcgc gacgggcgct accaggagtg caacatcggt
     1861 cgcggcttcg tcggccggct ggtccaggac gaatcgggcg gccgctgtcg ccgcgattcg
     1921 acgtggggca cccgcgacgg cgtgatctgg gtcaccaacg gctgctcagg tcgattcgag
     1981 cggctacgaa ccaagtactg acgcggctga tggggcgggc ccgatcagtg cccgccctgg
     2041 acgaacaggc ccgctgcgcg caacgcctcg gccagttgcc cttcgagccg cgcggcctcg
     2101 ccacgcggca gcc
//
LOCUS       DASPFR010000328         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_463492, whole genome shotgun sequence.
ACCESSION   DASPFR010000328 DASPFR010000000
VERSION     DASPFR010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463492"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="VD737_08685"
     CDS             <1..1320
                     /locus_tag="VD737_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10677.1"
                     /translation="SKLEALSNAEVGYFRPIPLDGSQLMIFHYAAVGFVPAIIEAQPT
                     EDLSAITFLGEQIATRHPVVQGWAAGPPSHVDYVAQVERQGAYRSSRELSREALYPIV
                     EGYKDSVALGGHARFSDPVGFDLLNFTLSYSPDSDLDSKERLHAAATYKHLLWSVGAR
                     WNAGDFYDLFGPTKRSREGWSAFFEYDRPLTYDPPEELRLVAKLAYFGDLDALPGFQN
                     VPSPTDKLGEVQVGLEYEYPRASVGKVDDETGYLWSLLAHGYEADGQFTPSLFGKLDV
                     GFPLQLRHSSIWLRTAAGIADGELEDPLANAYFGGFRNNYVDNGEAKRYRELLSMPGF
                     EIDALNGRSFVKGMLEWNLPPIRFEKLGSPGFFGSWVRPAVFTTALVTNPDSGAARVE
                     AYNVGFQFDLQLHVLNRLPMMLSFGYAKGFEGGGQGEDELMLSLKVL"
     gene            1357..>2188
                     /locus_tag="VD737_08690"
     CDS             1357..>2188
                     /locus_tag="VD737_08690"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HXW10678.1"
                     /translation="MVNGILRGTVALMPVLVFLGALVWFDSFKVVRARAILVAALAGM
                     AAAFAGYWINGFLLYALEIEYVDYARWVSPWLEEALKAALIVYLIRARRVGMIVDAAI
                     YGFAVGTGFALFENLYYLMMRPDTHPAVQVIRGFGTAIMHGGATAVFAIVSISLAERR
                     PDGWLRVFGPGLVGAALLHAVYNVLLFKPVFATLGILLLLPPLIYFTFEHSERSLRDW
                     LESDLDSDLQLLEAIHSGDFGDTHAGRYLHSLRDRFEGPVVADMLCYLRLHVELALRA
                     K"
BASE COUNT          331 a          749 c          721 g          387 t
ORIGIN      
        1 tcgaagctcg aggcgctcag caacgccgaa gtcggctatt tccggccgat acccctggac
       61 gggtcgcagc tgatgatctt tcactacgcg gccgtcggct tcgtgcccgc catcatcgag
      121 gcgcagccca ccgaggacct cagcgcgatc accttcctgg gcgaacagat cgcgacgcgc
      181 cacccggtcg tgcagggctg ggcggcaggc ccgccatccc acgtggacta cgtggcccag
      241 gtcgagcggc agggagccta tcgctcgtcg cgggagctct cgcgcgaggc gctctacccg
      301 atcgtcgagg gctacaagga ttccgtggcg ctcggcggcc acgcgcgctt cagcgatccg
      361 gtcggcttcg atctgctgaa cttcacgctg agctacagcc cggactcgga cctcgactcg
      421 aaagagcggc tgcatgccgc cgcgacgtac aagcacctgc tctggtcggt gggcgccagg
      481 tggaacgccg gcgacttcta cgacctgttc gggccgacga aacgtagtcg cgaaggctgg
      541 agcgcgttct tcgaatacga ccgcccactg acctacgacc cgcccgagga actccgcctg
      601 gtggcgaagt tggcctattt cggtgacctc gacgcgctgc cgggcttcca gaacgtcccc
      661 tcaccaacgg acaagctcgg cgaggtccag gtcgggctcg agtacgaata cccgcgcgcg
      721 tcggtcggca aggtcgatga cgaaaccggc tacctctggt cgctgctcgc ccacggctac
      781 gaggccgacg gccagttcac cccgagcctg ttcggcaagc tggacgtggg gttcccgctg
      841 cagctgcggc attcctcgat ctggctcagg acggccgccg gcatcgcaga cggcgaactc
      901 gaggacccgc tcgccaacgc ctacttcggg ggcttccgca acaactatgt cgacaacggc
      961 gaggccaagc gctaccgcga gctcctgagc atgccggggt tcgagatcga cgcgctcaac
     1021 ggccggtcgt tcgtcaaggg catgctcgag tggaacctgc ccccgatccg cttcgagaag
     1081 ctcggcagtc cgggattctt cggcagctgg gtccgcccgg ccgtgttcac gacggcgctc
     1141 gtcaccaacc cggactcagg tgccgcgcgc gtcgaggcct acaacgtcgg attccagttc
     1201 gacctgcagt tgcacgtgct gaatcgactg ccgatgatgc tgtcgttcgg ctacgcaaag
     1261 gggttcgagg gtggcggcca gggcgaagac gaactcatgc tgtccctcaa ggtgctctga
     1321 gcggcgccgg cgggcgcgac gaggcacggg tcgctgatgg tcaacggcat cctgcgcggc
     1381 acggtcgccc tgatgcccgt gctcgtcttc ctgggcgcgc tcgtgtggtt cgacagtttc
     1441 aaggtcgtgc gcgcccgcgc gatcctcgtc gcggcgctgg ccggcatggc ggccgcattc
     1501 gcgggttact ggatcaacgg tttcctgctc tacgcgctcg agatcgagta cgtcgactac
     1561 gcgcgatggg tgtcgccgtg gctcgaggaa gccctgaagg ccgcgctgat cgtgtacctg
     1621 atccgcgcgc ggcgggtcgg catgatcgtc gacgcggcga tctacggatt tgccgtcggc
     1681 accggcttcg cgctgttcga gaacctgtac tacctgatga tgcggccgga cacgcacccg
     1741 gccgtgcagg tcatccgtgg cttcggcacc gcgatcatgc acggcggcgc gaccgcggtc
     1801 ttcgcgatcg tctcgatctc gctggccgag cggcgccccg atggatggct tcgcgtgttc
     1861 ggtcccggcc tggtcggcgc ggcgctgctg catgccgtct acaacgtgct gctgttcaag
     1921 cccgtgttcg cgacgctcgg gatcctgctg ctgctgccgc cgctgatcta cttcacgttc
     1981 gagcacagcg aacggtcgtt gcgcgactgg ctcgagtccg acctcgactc cgacctgcag
     2041 ctgctcgagg cgatccactc cggcgatttc ggcgacacgc acgccggacg ctacctgcac
     2101 tcgctgcgcg accgcttcga gggcccggtg gtcgccgaca tgctctgcta cctgcggctg
     2161 cacgtcgaac tcgcgctgcg ggccaagg
//
LOCUS       DASPFR010000329        11879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_219439, whole genome shotgun sequence.
ACCESSION   DASPFR010000329 DASPFR010000000
VERSION     DASPFR010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11879
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219439"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VD737_08695"
     CDS             complement(<1..794)
                     /locus_tag="VD737_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXW10679.1"
                     /translation="MSDPLRTDALVIGCGIAGGTAALELAEAGARVVVVTRADRPEES
                     NTYWAQGGIIFRGEIDSPDLLAQDIVNAGAGLCHDQAVRTLAEAGPPLVQSILIDRLG
                     VPFDRLPDGRLSLGREGGHSVARIVHATDATGRAIETSIVEALQRHPNVTLLTDHTAV
                     DLLTPAHQGRDRRAVYDPLSCVGAYVLDQRTGDIDRVFARATVLATGGLGQIFLRTTN
                     PAGARGDGLAMAYRAGARVINTEYIQFHPTAFYHRNAPCFLITEAVR"
     gene            complement(801..1877)
                     /gene="nadA"
                     /locus_tag="VD737_08700"
     CDS             complement(801..1877)
                     /gene="nadA"
                     /locus_tag="VD737_08700"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HXW10680.1"
                     /translation="MHTAQDIYRDLQTKLGDVLPDAELLLKAEVAADVLRLKVERDAV
                     ILGHNYMEPALFHTVPDFQGDSLELSRRAAETDKSVIVFCGVRFMAETAKILNPTKTV
                     LLPTERGGCSLAESITAADVRALREAYPGVPIVTYINTYADVKAECDICCTSSNAAAV
                     VESLKSDTVIFLPDEYLARNVARETGRHIIVPSVFPRPPGAGNGHAAANGGEVELRMI
                     GWKGRCEVHELFTPQDVDDVRRQFPDVYILAHPECSPEVTAKADFSGSTTAMIKAVER
                     SNARRFLLLTECSMGDNIVAANPDKEMLRLCSHRCPHMHEITLEQTRDSLRYMRWQIE
                     VPEEIRVRALRSVERMLAVGPKVD"
     gene            complement(2019..3476)
                     /locus_tag="VD737_08705"
     CDS             complement(2019..3476)
                     /locus_tag="VD737_08705"
                     /inference="COORDINATES: protein motif:HMM:NF039533.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXW10681.1"
                     /translation="MERVLSRAGLTAFAAGATLFAASTTFAAPVPNKGGASAEHARVA
                     AYWTPERRAAAIPRDLVIDERGLGYLRKPDGSLQPYGHTIAAAAKDGAEPRAKPVGDN
                     TPPTISNLSPANGATIGASATFSAQVTDTSGVRSVSIRVGPSGGQQQSFNATQGAGDV
                     WSVNLSGFSSGPWTWSVVAKDKANNTATSATLGFTVSTSGGGGGGGGGGGVVTNGTWA
                     SGGVVQDAVGRIYFEMPSNSRLTRWAGYVCSGTVVTDGNTGRSVIQTAAHCVYDDAYK
                     AFARNVLFIPYQDGTNGSGTDLNCANDPYGCWSTAFGVVDTDWTTRTFPNNVAWDYAY
                     YVVANSGAHEPGLADRGDALDGAVTPMTVKFTAPTVGAYTHALGYSYSEDPNFKYCAE
                     NLANLDSADWWLGSCGLSGGSSGGPWIQPLTDGDGPLVSVNSWGYTNQPGMAGPKLSG
                     SSAQCVFNAATSNTQTPFFGAAPADGSAGYVAPGC"
     gene            complement(3548..5059)
                     /locus_tag="VD737_08710"
     CDS             complement(3548..5059)
                     /locus_tag="VD737_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HXW10682.1"
                     /translation="MGGAQRSLLERGVLAAEQGRVREGLALIRQAITGTPDDPEAHAQ
                     LGRWLSRLHRQADAEAAVARALELGPRSARTFDTAGVVLSRGGRHARAVECFARATAL
                     EPVNAGFQFNLASSLKFLGRFAEAEAAYEACLVANPRYWRAHSALSQLSRQTPDRNHL
                     ARLQALLAEGGLDVDAELHLRQALARELEDLGRYDEAFQHLLAGRSRKRAASRYTFAR
                     DAELFQAVTRTTPEPLPLDGQATQNAPIFVVGMPRTGTTLLERMLASHSQVASVGESQ
                     NFAVLLKRATETRTPTVLDPQTIAAAAHVDHAALGRSYLDHTRPRGARPRFVDKMPLN
                     FFYLGHIARALPGARIVVLRRHPLDTVVASFRQLFATTMPYYEYAYDLGDAARYWLAF
                     DALIAHWRRVLPGRLVEVEYESLVRDPAGALRPVLEHCGLDWEARCLEFDRNRDAVAT
                     ASAVQVRQPVYASSVGRWRHYQAHLEPALRVFATAGLTWAVDAGEPDSEGSFP"
     gene            complement(5074..7473)
                     /locus_tag="VD737_08715"
     CDS             complement(5074..7473)
                     /locus_tag="VD737_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXW10683.1"
                     /translation="MQESQGRGRSSRLAAAVAAALGAGALATPAGAQQAYTDEGVPLE
                     TIIVTATRRDVNVQDVPFNIVALGPDSLADLRIDNLAEFTRAVPGLYVPNQGPRGSNL
                     VTARGLNVTSLNDTALIGNGTGGTVATYLGDIPLYLDLKMIDVERVEALLGPQGTLYG
                     AGTLAGAIRYLPNRPDPTRWAAEAGGRVYSLDQSDGTGVDAWGMINVPLVQDRLALRV
                     AAGYIDDPGFVDYRYVVREPGVSNPNAPFDDSGDDVAANLRKVDDVDTEETFTGRVGL
                     SWQLTDALGANFTYYYQDQQVGGRTVNHDEALGVGRYVSGARFLEPNDKTNHLFALEL
                     TWDLGFAELTSATGVSRFESFGTRDQTDFLMDLDYGYEDFPNFAAYATDDIEEDTFNQ
                     ELRLVSQGTEKWSWIVGAFYNRYEGDTIAAEHTPGIPEWYGLDADFPDLEYRATSERT
                     FEETALFGELGYRFTDRWQVTVGARWFDYQDELSLVTEFPFLDTVLPDQNDTGDDETI
                     FKINTSFEITGDVMTYLTISEGYRQGGLNSGPPCQDPIPDEQTFCLAPDEVLIKPDTT
                     TNYELGLRSLWFDGRLLLNAAIYQIDWKDIQVIGTSDVGEVPIIVNGGTAETRGLEVT
                     TRWSITDSLQLAASFALNDAQLTSDAPGLVDGADAFDGDRLAGTPEKQGTLLLDYERP
                     LANGWTMHANYSLTAVSDVYTKVGGPRNGGEVLPGYAVHGAAIGLSTGAWTLTLYGDN
                     VFDKYAVTSVRRDPSYIYDVERIDPVTEEPIASIPSRTYFQSMLRPRTVGLEVSYRFD
                     L"
     gene            7701..10043
                     /locus_tag="VD737_08720"
     CDS             7701..10043
                     /locus_tag="VD737_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXW10684.1"
                     /translation="MTRSRWTRWLPLLYLVFGITWILGSDAVVGRMFGDSPYLLLVVS
                     TFKGFAFVALTALLLAVWWRAEQRHNRALVAGAEARETFANERMAWMSRHAHDVILLL
                     DDDGQIIDCNERAEVTYGYPQAELVGMSVFDLRADVGRPAADSQFREVLARGALVFQA
                     EHRTRDGQVFPVEVSSRSVEFQGRTYVQSIIRDQRELVAVRRRVERQRDLYDMLSRCN
                     HAIARLRDPALLYQAVTRVAVEHGHFLFAWIAGKRPDGSVVYEAAFGDDGGHLSDLGV
                     TTRADDPRSQGPTGRALREGSTIVVNRFFEDARTQPWHDVAQRVGIRSSASVAIRVRG
                     SVVAALTVYAAEPDFFVAEIVRTLEEVAAEIGFGLETLQVRKELDESRNLLQTVIDAT
                     ATPIYAFDRSGRALLMNEAGARIMGLSRESILGHSRLLRWTPEEASAHAASDERVFAS
                     GETVYVEETVGSGPAERTFLSVKFPLRDLDGRIYAVGGVSTEITELRRAQHAVVEANA
                     GLEHVVAERTQQLVEARDRAEQADRAKTTFLSTISHELRTPLNSIIGFTEIVLRELSG
                     PLNEEQRRQLTIVQDSSRLLLDLINEILDLSRIEAGRMQFSLEPFDLAELLQRQVDSL
                     ASQADAKKLELRCQIGPDVREMVSDAKRVTQIVANLLANAIKFTERGGIAVVAERADA
                     RRVRIEVRDSGPGIAPADLTHLFKPFVQVGQAQRQHREGTGLGLAISRHLARALGGDI
                     EVASELGRGTTFTVVLPLDARTSVKDGEESGLFRRLDAAT"
     gene            complement(10062..>11879)
                     /locus_tag="VD737_08725"
     CDS             complement(10062..>11879)
                     /locus_tag="VD737_08725"
                     /inference="COORDINATES: protein motif:HMM:NF024763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HXW10685.1"
                     /translation="SDLAATSGGATFERDMASVYAALQADGELVGCDAALPGRFVRHA
                     WAAVQRDKASLARARIHRLVVRLDEVLRADAARSPAAHAPERLQAGFGEAHRGLFDFA
                     AMSRLLHHAAPHATLAPARRQRIVAALEVLRSQRFYPDPDGAPDAAEWVFDDIGSALD
                     AFLARTPAMVQLHKTLLVAELEADGLYVEALHDPILAAIDETSLTPADLQFFPDFLVC
                     TNSTSRANVALLEALSAGVPVKVVVQVGDLIERSSAGLGRFAFGLREAHLAQAAMSLE
                     NVYTLQTAASNLLQMRDAVQRGLRHAGPALFAVYAPPADAGEDGLPPYLGAAAAMQSR
                     AFPAWCHDPGAGTDLATRFSLANNPQVAEDWPVESLRYADPDLQSVTERVPFTFVDFA
                     LCDRRYDGHFAVIPRAEWGPGQVAAAQWLANPPEDASSCVPYVLAVDEAGTLCRVVVH
                     DALMRAALRCRESWRRLRELAGIGDSRVERALAREREARSSEGATAGGSAPIPETEAV
                     TATAVAPAATEVDRDPREPYVETLRCSSCNECTLAFPKIFAYNDDKQAYIRDVRAATY
                     RQLVEAAESCQVSVIHPGQPRDATEPGLEELIERARPFL"
BASE COUNT         1784 a         4104 c         4149 g         1842 t
ORIGIN      
        1 cgcaccgctt ccgtgatcag gaagcacggc gcattgcggt ggtagaacgc cgtcgggtgg
       61 aactgtatgt actcggtgtt gatcacgcgc gcgccggccc ggtacgccat ggcgagcccg
      121 tcgccgcgcg cgccggccgg attcgtcgtg cgcaggaaga tctgcccgag cccgccggtc
      181 gccagcaccg tcgcgcgcgc gaacacgcgg tcgatgtcgc ccgtacgctg gtcgagcacg
      241 tacgcgccga cgcacgagag cggatcgtag accgcgcgac ggtcacgccc ctggtgcgcg
      301 ggcgtcagca ggtcgacggc ggtgtgatcg gtcagcagcg tgacgttcgg atggcgctgc
      361 agcgcctcga cgatcgaggt ctcgatcgcg cgcccggtcg cgtcggtggc gtgcacgatg
      421 cgcgccaccg aatgaccacc ctcgcgccca agcgaaagcc gtccgtcggg cagccggtcg
      481 aacggcacgc cgagccggtc gatcaggatc gactgcacga gcggcgggcc ggcctcggcg
      541 agcgtgcgca cggcctgatc gtggcagagg cccgctcccg cgttgacgat gtcctgggcg
      601 agcaggtcgg gcgagtcgat ctcgccgcgg aagatgatgc cgccctgcgc ccagtaggtg
      661 ttggattcct cggggcggtc ggcccgcgtc accacgacca ccctcgcccc ggcttcggcc
      721 agctcgaggg cggccgtgcc gcccgcgatg ccacacccga tgaccagggc gtcggtgcgc
      781 agcgggtcac tcatcgggca ctaatcgacc ttcgggccca cggcgagcat gcgctcgacc
      841 gagcgcagcg cgcgcacacg gatctcctcc ggaacttcga tctgccagcg catgtagcgc
      901 agcgagtcgc gcgtctgctc gagcgtgatc tcgtgcatgt gcggacaacg gtggctgcag
      961 agccgcagca tctccttgtc ggggttcgcg gcgacgatgt tgtcgcccat cgagcactcc
     1021 gtgagcagca ggaagcgtct ggcgttcgag cgttccaccg ccttgatcat cgcggtggtg
     1081 ctgccggaga agtctgcctt ggcggtcacc tcggggctgc actccggatg cgccaggatg
     1141 tagacgtccg ggaactgcct gcgcacgtcg tcgacgtcct ggggcgtgaa cagctcgtgc
     1201 acttcgcagc ggcccttcca gccgatcatc ctgagctcga cttcgccacc gttggccgcc
     1261 gcgtggccgt tgccggcgcc gggcggcctg gggaacacgg aaggcacgat gatgtggcga
     1321 ccggtttcac gagcgacgtt gcgtgcgaga tattcatccg gcaggaagat caccgtgtcg
     1381 gacttcagcg actcgaccac tgcggccgcg ttgctcgacg tgcagcagat gtcgcactcg
     1441 gctttcacgt cggcataggt gttgatgtac gtcacgatcg gcacgcccgg gtacgcttcg
     1501 cgcagcgcgc gcacgtcggc ggccgtgatg ctctccgcga gcgagcagcc accgcgctcg
     1561 gtcggcagca ggaccgtctt ggtcgggttg aggatcttcg cggtctccgc catgaaacgg
     1621 acgccgcaga acacgatgac ggacttgtcg gtctcggcgg cgcgacggct gagctccagc
     1681 gagtcgccct ggaagtccgg caccgtgtgg aacagcgcgg gctccatgta gttgtgaccg
     1741 aggatgacgg cgtcgcgctc gaccttgagg cgcaggacgt cggcggccac ttcggccttg
     1801 agcagcagtt cggcgtccgg gagcacgtcg ccgagcttgg tctggaggtc gcggtagatg
     1861 tcttgggcag tgtgcacgat ggctgtccgg gggccgccgg ggcggtcgga cctgggattt
     1921 tagccgacag ccggaccccc ggcgccgctt acgtccaaaa cgaacgggcc gggcgcctcg
     1981 cgacgcccgg cccgggactt gtgctccctg accagttctc agcagccggg cgccacgtac
     2041 cctgcgctgc cgtccgcggg cgccgccccg aagaacggcg tctgcgtatt gctggtcgcc
     2101 gcgttgaaca cgcactgcgc gctcgacccg ctcagcttcg gccccgccat gccgggctga
     2161 ttcgtgtagc cccaggaatt cacggagacc agcgggccgt caccatccgt cagcggctgg
     2221 atccacgggc cgcccgagga gccacccgag aggccgcagc tgccgagcca ccagtcggcc
     2281 gagtcgaggt tggcgaggtt ctcggcgcag tacttgaagt tcgggtcctc gctgtacgag
     2341 tagccgagcg cgtgcgtgta cgcgcccacg gtcggtgcag tgaacttgac cgtcatcgga
     2401 gtgacggcgc cgtcgagcgc gtcgccacgg tccgcgagcc cgggctcatg cgcgccgctg
     2461 ttcgcgacga cgtagtaggc atagtcccag gcgacgttgt tcgggaacgt gcgcgtcgtc
     2521 cagtccgtgt cgaccacgcc gaaagctgtc gaccagcagc catacgggtc gttcgcgcag
     2581 ttgagatcgg tgcccgatcc attcgtgccg tcctggtacg gaatgaacag gacgttgcgt
     2641 gcgaacgcct tgtacgcgtc gtcgtagacg cagtgcgccg ccgtctggat gaccgatcga
     2701 ccggtgttgc catccgtcac gaccgtgccc gaacagacat aacccgccca gcgcgtcagg
     2761 cgggaattgc tcggcatctc gaagtagatc cggcccaccg cgtcctgcac gactccgccc
     2821 gacgcccacg tgccgttggt gacgactccg cccccaccgc caccgccgcc gccaccgcct
     2881 gacgtgctga cggtgaaccc gagcgtcgcg ctcgtggcgg tgttgttggc cttgtccttc
     2941 gcgacgactg accaggtcca aggtccgctg ctgaagcccg acaggttgac cgaccacacg
     3001 tcaccagcgc cctgggtcgc gttgaacgac tgctgctggc cgccgctcgg ccccactcgg
     3061 atgctgaccg agcggacgcc ggacgtgtcg gtcacctggg ccgaaaacgt cgcggacgcg
     3121 ccgatcgtcg cgccattggc cgggctcagg ttggagatcg tcggtggcgt gttgtcaccg
     3181 acgggcttcg cccgcggttc cgcgccatcc ttcgcggcgg ctgcgatcgt atgaccgtag
     3241 ggctgcagcg aaccatcggg cttccgcaga taaccgagtc cgcgctcgtc gatcacgagg
     3301 tcgcgcggga tcgccgctgc acggcgttcc ggcgtccagt aggcggcaac gcgggcgtgc
     3361 tcagcgctgg cgccgccttt gttcggaacg ggtgcggcga acgtcgtcga tgccgcgaag
     3421 agcgtggctc ccgcagcgaa ggccgtgagc ccggctctac tcagaactcg ttccatcgga
     3481 ggcattcctc ggcgtgacgg gagcgcgaat acagcagaga aacgggacct tgtccacgat
     3541 cccgggatta tggaaaagag ccttcgctgt ccggttcgcc tgcgtcgacg gcccaggtga
     3601 ggccggccgt agcgaagact ctcagcgcgg gctcgaggtg tgcctggtaa tggcgccacc
     3661 gtccgacgct gctcgcatac accggttgcc gcacctgcac cgcgctcgcg gtcgccacgg
     3721 cgtcccggtt ccggtcgaac tcgaggcaac gcgcctccca atcgagtccg cagtgctcga
     3781 gcacgggtcg cagcgcaccc gctggatccc gtaccagcga ctcgtactcg acctcgacga
     3841 gccgccccgg cagcaccctc ctccagtgcg caatcaacgc gtcgaacgcg agccagtagc
     3901 gcgccgcgtc gccgaggtcg taggcatact cgtagtacgg catggtggtc gcgaacaact
     3961 ggcggaagct cgcgacgacg gtgtcgagcg gatgccgacg cagcacgacg atgcgcgcgc
     4021 ccggcagcgc gcgggcgatg tgcccgaggt agaaaaagtt gagcggcatc ttgtcgacga
     4081 agcgcggccg cgcgcctcgc ggcctcgtgt gatcgaggta cgatcggccc agcgcggcgt
     4141 gatcgacgtg ggccgcggca gcgatcgtct gcggatccag caccgtcggc gtccgcgtct
     4201 ccgtggcgcg cttgagcagc accgcgaagt tctgcgactc gccgaccgag gccacctgcg
     4261 agtggctcgc gagcatgcgc tcgagcaggg tcgtgccggt gcgcggcatg ccgaccacga
     4321 agatcggcgc gttctgcgtc gcctgtccgt cgagcggcag cggttcgggc gtcgtgcgcg
     4381 tcacggcctg gaacagctcc gcgtcgcgcg caaacgtgta gcgactcgcg gcgcgcttgc
     4441 gggaccgccc cgcgagcaga tgctggaaag cctcgtcgta gcggccgagg tcttcgagtt
     4501 ccctggcgag cgcctgccgc aggtgcagtt ccgcgtccac gtccagtccg ccttcggcga
     4561 gcagtgcctg caggcgcgca aggtgattgc gatcgggcgt ctgccgggac agctgcgaaa
     4621 gcgccgagtg ggcgcgccag tagcgcgggt tcgccaccag gcaggcctcg taggccgcct
     4681 ccgcctcggc gaagcggccg aggaacttga gcgacgaggc caggttgaac tggaagccgg
     4741 cgttgacggg ctcgagcgcg gtggcgcgcg cgaagcactc gaccgcgcgt gcgtgacggc
     4801 cgcccctgct cagcacgacg cccgccgtgt cgaacgtgcg cgcgctcctc ggaccgagct
     4861 cgagggcacg cgcgaccgct gcttcggcgt cagcctggcg atgcagccgc gacagccagc
     4921 gtccgagctg cgcatgagct tccggatcgt ccggcgtgcc ggtgatcgcc tggcggatca
     4981 gcgccaggcc ttcgcggacg cgcccctgct ccgccgcgag cacgccacgc tcgagcaggg
     5041 atcgctgcgc accgcccacc agcgcggccc gcttcagagg tcgaatcgat aggagacctc
     5101 gaggccgacg gtgcgtggtc gcagcatgct ctggaaatac gtgcgcgacg ggatcgacgc
     5161 aatcggctcc tcggtgaccg gatcgatgcg ctccacgtcg tagatgtagc tcgggtcacg
     5221 ccggaccgaa gtcaccgcgt acttgtcgaa cacgttgtcg ccgtaaagcg tgagcgtcca
     5281 cgcaccggtg ctcaggccga tggcggcgcc atgcacggcg tagccgggca gcacctcgcc
     5341 gccgtttcgc ggcccgccca ccttggtgta aacgtcgctg accgcggtga gcgagtagtt
     5401 ggcgtgcatc gtccagccgt tcgccagcgg tcgttcgtaa tccagcagca gcgtgccctg
     5461 tttctccggc gtccccgcga gccggtcgcc gtcgaacgcg tcggcgccgt ccacgagtcc
     5521 cggagcgtcg ctcgtcagct gcgcgtcgtt gagcgcgaag ctcgcggcca gctgcagcga
     5581 atcggtgatg ctccagcgcg tcgtcacctc gaggccgcgc gtctcggcgg tgccgccgtt
     5641 gacgatgatc ggcacctcgc cgacgtcgct ggtgccgatg acctggatgt ccttccagtc
     5701 gatctggtag atcgcggcgt tgagcagcag ccgcccatcg aaccacaggc tgcgcaggcc
     5761 cagttcgtag ttggtcgtcg tgtcgggctt gatcaggacc tcgtcgggcg ccaggcagaa
     5821 cgtctgctcg tcaggtatcg ggtcctggca cggcggcccg gagttgagcc ccccctgccg
     5881 gtagccttcg ctgatcgtca ggtaggtcat gacgtcgccc gtgatctcga acgacgtgtt
     5941 gatcttgaag atggtctcgt cgtcgccggt gtcattctgg tccggcagca cggtgtcgag
     6001 gaacggaaac tccgtgacca gcgacagctc gtcctggtag tcgaaccagc gcgcccccac
     6061 cgtcacctgc caccgatcgg tgaaccggta accgagctcg ccgaacagcg cggtctcctc
     6121 gaacgtccgt tccgacgtcg cccggtactc gaggtcgggg aaatcggcat ccaggccgta
     6181 ccactcgggg atgcccggcg tgtgctccgc ggcgatcgtg tccccctcgt agcggttgta
     6241 gaacgcgccg acgatccagc tccacttctc cgtgccctgc gacacgagac gcagctcctg
     6301 gttgaaggtg tcctcctcga tgtcgtcggt ggcataggcc gcgaagttcg ggaaatcctc
     6361 gtagccgtag tcgaggtcca tcaggaaatc ggtctggtcg cgcgtgccga acgactcgaa
     6421 gcgggaaact cccgtcgcgg aggtgagttc cgcgaaaccg aggtcccacg tcagttcgag
     6481 cgcgaacaaa tggttggtct tgtcgttggg ctcgaggaag cgcgcgccgg agacgtagcg
     6541 gccgacgccg agcgcctcgt cgtggttgac cgtgcggccg ccgacctgct ggtcctggta
     6601 gtagtacgtg aagttggcgc ccagcgcgtc cgtgagctgc cacgacaggc cgacgcggcc
     6661 cgtgaaggtt tcctcggtat cgacgtcgtc gaccttgcgc aggttggcgg ccacgtcgtc
     6721 gcccgagtcg tcgaacggtg cattcgggtt cgagacgccc ggctcgcgca cgacgtagcg
     6781 gtagtcgacg aaacccgggt cgtcgatgta gcccgcggcg acgcgcaggg ccagccggtc
     6841 ctgcaccagg ggcacgttga tcatgcccca ggcgtcgacg cccgtgccat cgctctggtc
     6901 gagcgagtag acgcgcccgc cggcttctgc cgcccagcgg gtggggtcgg gccggttcgg
     6961 caggtagcga atcgcacccg cgagcgtccc cgcaccgtac agcgttccct gcggcccgag
     7021 cagcgcctcg acccgctcga cgtcgatcat cttgaggtcc aggtacagcg ggatgtcgcc
     7081 gaggtaggtc gccaccgtcc cgccggtgcc gttgccgatc aacgccgtat cgttgagcga
     7141 ggtcacgttg aggccccggg cggtgacgag gttgctgccg cgtggcccct ggttcggcac
     7201 gtagaggccc ggcaccgccc gcgtgaattc ggccaggttg tcgatgcgca ggtccgcgag
     7261 cgagtcggga ccgagcgcga cgatgttgaa cggcacgtcc tgcacgttca cgtcgcggcg
     7321 cgtcgccgtg acgatgatgg tctcgagcgg cacgccctcg tcggtgtacg cctgctgggc
     7381 tccggccggc gtcgccagcg cgccggcccc cagcgccgcc gcaacggccg ccgccagtcg
     7441 tgacgaccgg cccctcccct ggctctcctg catcgcccct ccttcccaag gacgttctgg
     7501 cgacacgccc cgcttttcac gcgggacggc atcgcgacgc agcctcgtat atccgaaagc
     7561 gcaccggctt tcaaacagct acgctacgac ggcaccatgg accacgcgat ccccagggac
     7621 ggattcggat gcgagcgcga tgcgttgcgg ccgaacggca tgcggcaccg gcgcgcgggg
     7681 ccggccccat gaccgccccc atgaccaggt cgcgctggac ccgctggctg ccgctgctct
     7741 acctcgtctt cggcatcacg tggatcctcg ggtcggatgc ggtcgtcggc cgcatgttcg
     7801 gggacagccc ctacctgctg ctggtcgtca gcaccttcaa gggcttcgca ttcgtcgcgc
     7861 tgacggcact gctgctggcg gtctggtggc gcgccgaaca gcgccacaat cgcgcgctgg
     7921 ttgcaggagc cgaggcccgg gagacgttcg cgaacgaacg catggcctgg atgagccgtc
     7981 acgcgcacga cgtgatcctg ctgctcgacg acgacgggca gatcatcgac tgcaacgagc
     8041 gcgcggaggt cacgtacggg tacccgcagg cggaactcgt cggcatgagc gtgttcgacc
     8101 tgcgcgcgga cgtgggccgg ccggcggccg acagccagtt tcgcgaggtg ctcgctcgcg
     8161 gcgcgctggt cttccaggcc gagcatcgca cccgcgacgg gcaggtcttc ccggtggagg
     8221 tcagctcgcg cagcgtggag ttccaggggc gcacgtacgt acagagcatc atccgggacc
     8281 agcgtgaact ggtggccgtg cgtcgtcgcg tggagcgcca gcgcgacctc tacgacatgc
     8341 tctcgcggtg caaccacgcc atcgcgcgcc tgcgcgatcc cgcgctgctg taccaggccg
     8401 tgacgcgggt ggcggtcgag cacggccact tcctgttcgc gtggattgcg ggcaagcgac
     8461 cggacggttc ggtggtgtac gaggccgcgt tcggcgacga cggtggtcac ctgtccgacc
     8521 tcggggtcac cacgcgcgcg gacgacccgc gcagccaggg tccgacggga cgcgccctgc
     8581 gcgaaggaag cacgatcgtc gtcaaccggt tcttcgagga tgcgcggacc cagccgtggc
     8641 acgacgtcgc gcagcgcgtg ggcatccgct cgtcggccag cgtggcgatc cgcgtgcggg
     8701 gcagcgtcgt cgccgcgctg accgtgtatg ccgcggagcc cgatttcttc gtcgcggaga
     8761 tcgtccgcac gctcgaggaa gtcgcggccg agatcggctt cgggctcgag acgctccagg
     8821 tccgaaagga gctcgacgag agccgcaacc tgctgcagac ggtcatcgac gcgacggcga
     8881 cgccgatcta cgcgttcgac cgcagcggcc gcgcgctgct gatgaacgag gccggggccc
     8941 gcatcatggg actctcgcgc gagagcatcc tcggtcactc ccgtctgctg cgctggaccc
     9001 cggaggaagc ctcggcccac gcggcgtccg acgagcgcgt ctttgcctcg ggcgaaaccg
     9061 tctatgtcga ggagaccgtc ggcagcgggc ccgcggagcg cacgttcctg tcggtcaagt
     9121 ttccgctgcg cgacctcgac gggcggatct acgccgtcgg cggcgtgtcg accgagatca
     9181 ccgagctgcg tcgtgcccag cacgccgtcg tcgaggccaa cgccggcctg gagcacgtgg
     9241 tcgcggagcg cacgcagcag ctcgtcgagg cgcgtgaccg cgcggagcag gccgaccgcg
     9301 cgaagaccac tttcctttcg acgatctcgc acgagctgcg cacgccgctc aactcgatca
     9361 tcggcttcac cgagatcgtg ctgcgcgagc tgtccgggcc gctcaacgaa gagcagcgcc
     9421 gccagctcac gatcgttcag gactcctcgc gcctgctgct cgacctgatc aacgagatcc
     9481 tcgacctctc gcggatcgag gccgggcgca tgcagttcag cctcgagccg ttcgacctgg
     9541 ccgagctgct gcagcgacag gtggattcgc tggcctccca ggccgacgcc aagaagctgg
     9601 agctgcggtg ccagatcggc cccgacgtca gggagatggt cagcgacgcc aagcgtgtca
     9661 cgcagatcgt cgcgaacctg ctcgccaacg cgatcaagtt caccgaaagg ggcggcatcg
     9721 cggtggtcgc cgaacgggcg gacgcccgcc gcgtgcgcat cgaggtccgc gactcgggcc
     9781 cgggcatcgc gccggcggac ctgacgcacc tgttcaagcc gttcgtgcag gtggggcagg
     9841 cgcagcggca gcaccgcgag ggcacgggac tgggactcgc gatttctcgt cacctggccc
     9901 gcgcgctggg cggcgacatc gaggtcgcga gcgaactcgg ccgcggcacc acgttcacgg
     9961 tcgtgttgcc gctcgacgca aggacgtccg tcaaggacgg cgaggaatcc gggctgttcc
    10021 ggcgcctcga cgcggcgacc tgagcgcggg tcgcggcgac ttcaaaggaa cggcctggcc
    10081 cgctcgatca gttcctcgag acccggctcc gtggcgtcgc gcggctggcc ggggtggatg
    10141 acggagacct ggcacgactc ggcggcctcg accagctggc ggtacgtcgc cgcgcggacg
    10201 tcgcggatgt acgcctgctt gtcgtcgttg taggcaaaga tcttcggaaa cgcgagcgtg
    10261 cactcgttgc agctcgagca gcgcagcgtc tcgacgtaag gctcgcgcgg atcgcggtcc
    10321 acctccgtcg ccgccggggc gacggcggtc gccgtcacgg cttcggtctc cgggatcggc
    10381 gccgagccgc ccgcggtggc cccttcggag gatcgcgcct cccgctcccg tgcgagtgcc
    10441 cgctccaccc gcgaatcgcc aatgcccgcc agctcccgca ggcgccgcca ggactcgcgg
    10501 cagcgcagcg cggcgcgcat gagggcgtcg tgcacgacga cgcggcacaa cgtgcccgcc
    10561 tcgtcgaccg ccagcacgta cggcacgcac gacgaagcgt cctcgggagg attcgccagc
    10621 cattgcgccg ccgcgacctg gcccggcccc cactccgcgc gtggaatcac ggcgaaatgt
    10681 ccgtcgtagc ggcgatcgca cagcgcgaag tccacgaacg tgaagggcac gcgttccgtc
    10741 accgactgca ggtccgggtc cgcgtaccgc agcgactcga caggccagtc ctcggcgacc
    10801 tgcggattgt tggccagcga gaaccgcgtc gcgaggtcgg ttccggcgcc cggatcgtga
    10861 caccaggccg gaaacgcgcg ggactgcatg gccgccgccg cgccgagata gggcggcagc
    10921 ccgtcctccc ctgcgtcggc gggaggcgca tagaccgcga acagggccgg gcccgcgtgc
    10981 cgcaggccac gctgcacggc gtcgcgcatc tgcagcaggt tcgatgccgc cgtctgcagg
    11041 gtgtagacgt tctcgaggct catggccgcc tgggccaggt gtgcctcgcg cagtccgaac
    11101 gcgaagcgtc cgagcccggc cgacgaccgc tcgatcaggt cgcccacctg cacgacgacc
    11161 ttgaccggca cgcccgcgga gagtgcctcg agcaacgcga cgttggcgcg cgacgtcgaa
    11221 ttcgtgcaga cgaggaaatc cgggaagaac tgcaggtcgg cgggggtcag cgaggtctcg
    11281 tcgatggccg cgaggatcgg gtcgtgcagc gcttccacgt ataggccgtc ggcctcgagt
    11341 tccgcgacca gcagggtctt gtgcaactgc accatggcgg gcgtgcgggc caggaacgcg
    11401 tcgagcgccg agccgatgtc gtcgaagacc cactcggccg cgtccggcgc gccgtccggg
    11461 tcggggtaga agcgttgcga gcgcagcact tcgagtgcgg cgacgatccg ctgccggcga
    11521 gcgggcgcga gcgtggcatg cggtgccgcg tgatgcagca atcgtgacat cgccgcgaag
    11581 tcgaacaggc ctcgatgcgc ctcgccgaac ccggcctgca ggcgctccgg ggcgtgtgcg
    11641 gccggcgagc gcgcggcatc ggcgcgcagc acctcgtcga gccgcacgac caggcggtgg
    11701 atccgcgcac gcgcgagact cgccttgtcg cgctgcacgg cggcccacgc gtggcgcacg
    11761 aagcggcccg gcaacgcggc atcgcagccg accagttccc cgtcggcctg cagcgcggcg
    11821 tacacggagg ccatgtcgcg ttcgaacgtc gcgcctcccg aagtggccgc gaggtccga
//
LOCUS       DASPFR010000330         5138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1562013, whole genome shotgun sequence.
ACCESSION   DASPFR010000330 DASPFR010000000
VERSION     DASPFR010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562013"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1723
                     /locus_tag="VD737_08730"
     CDS             <1..1723
                     /locus_tag="VD737_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXW10686.1"
                     /translation="ANEARCNAPGAKGAFFVERIWLKHYPPGVPADINPAEHPSLLHL
                     LERSIERHRDRPCFTCMDRTLTYGELDVLARRFGTYLQRVARLRPGDRIAIMLPNVLQ
                     YPVAILGAFRAGLTVVNTNPMYTPRELRHQLADSGARAILILENFAHTLEQVIDDVDL
                     DTVIVTSVGDLLGFPKSVLVDFALRHLRKQVPDWSLPGAIGFGDALNQGRFENATPVA
                     LGHEDVAFLQYTGGTTGVSKGAVLTHRNMVANVLQAEAWIKPALKPGEPMVSITALPL
                     YHIFALTANSFTMLKLGAHNVMIPNPRDFPGFVRELSKHRFTFFAGVNTLFNALLHTP
                     GFDRLDFSTLRISLGGGMAVQRAVAENWKRVTGNVLTQAWGLTETSPAACINRPGDDF
                     NGSIGLPIPSTLVTIRDDAGNVLPQGQSGEICVEGPQVMRGYWNRPDETENVMLPGQV
                     LRTGDVGHMDPDGFVYIEDRKKDMILVSGFNVYPNEVEGVAVTHAGVLEVAAVAQADE
                     RAGEVVALFVVRKDPKLGEQELIDHCRASLTGYKVPKRVYFRDELPKTNVGKILRREL
                     KDQLKTS"
     gene            1806..3176
                     /locus_tag="VD737_08735"
     CDS             1806..3176
                     /locus_tag="VD737_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXW10687.1"
                     /translation="MADSVLSLIGNTPLVRVRSFETGPCELYLKLESQNPGGSIKDRI
                     GLSMIEAAERSGRLQPGATIVEATAGNTGLGLALVAAQKGYRLVLVVPDKMSREKILH
                     LAALGADVRLTRSDVGKGHPQYYQDMAQRIAAEMPGAYYINQFGNPANPLAHETTTGP
                     EILRQMDGRVDAIVCGVGSSGTVTGLSHVFARESPGTRFVLADPVGSVLASYVNTGVL
                     GQAGSWAVEGIGEDFIPAIADLSRVSVAYSIPDAESMATVRALLRQEAIFGGSSTGTL
                     LAAALRYCREQTQPQRVVTFVCDGGAKYLSKAYNDAWMYERGFLEREQSGDLRDLIVR
                     RHDAGLTITVQPGDSLLTAYNRMRSADVSQLPVVEEDGRIVGILDEFDLLQVVRRADA
                     AERFQQPVRTAMETRLETLEPDAGLDALFAVLDAGHVALIVERGTFLGLVTRIDLLNA
                     LRLRTL"
     gene            3173..4351
                     /locus_tag="VD737_08740"
     CDS             3173..4351
                     /locus_tag="VD737_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HXW10688.1"
                     /translation="MTDHDRNARPRFATRAIHAGQEPDPATGAVMVPIYATSTYVQSS
                     PGVHKGFDYGRSHNPTRFAFERCVADLENGSQAFAFASGLAAIGTLLECLDAGSHIVT
                     TDDLYGGTVRLLERVRQRSMNLSSTRVDFTDLSAVERAIRPETRLLWVESPTNPTLKL
                     ADLEAVAALARRRGLLAVADNTFASPWVQRPLDLGFDAVVHSTTKYLNGHSDMIGGVA
                     VVGQNAELRERLGFLQNAVGGISGPFDSFLALRGLKTLAVRMQRHCENALAIAQWLER
                     QGKVRRVLYPGLPSHPQHALALRQTPRFGGMLSVEFDTDLAGARRLLERCRIFALAES
                     LGGVESLIEHPALMTHSQLAPERRQSLGIGDGLIRISAGIEDVEDLIGDLEQAMRALG
                     "
     gene            complement(4427..5020)
                     /gene="ppa"
                     /locus_tag="VD737_08745"
     CDS             complement(4427..5020)
                     /gene="ppa"
                     /locus_tag="VD737_08745"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF002317.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HXW10689.1"
                     /translation="MDISKISIGPSPPRDVNVLIEIPQGGVPVKYELDKESGALRVDR
                     FLNTAMFYPGNYGFIPHTLSGDGDPVDVIVVVPSPVVAGAIVRVRPVGALLMKDEAGD
                     DEKIIAVPVDKLHPFYSGVKSYRDLPATLTDQIAHFFQHYKDLEKGKWVTIVNWVGTD
                     KAERMIVEGIRRAAQAGDDVAAHYVASGALKASARRS"
BASE COUNT          833 a         1752 c         1749 g          804 t
ORIGIN      
        1 cgcgaacgag gcgcgctgca acgcgcccgg ggccaaggga gcgttcttcg tggaaaggat
       61 ctggctgaag cactacccgc ccggagtccc ggcggacatc aacccggccg agcatccgtc
      121 gctgctgcac ctgctcgagc gcagcatcga gcgtcaccgc gaccgccctt gcttcacctg
      181 catggaccgg acgctgacct acggcgagct cgacgtgctc gccaggcgat tcggcaccta
      241 cctgcagcgc gtggcgcggc tgcgtcccgg cgaccgcatc gcgatcatgc tgccgaacgt
      301 gctgcagtac ccggtcgcga tcctgggtgc cttccgcgca gggctcacgg tcgtcaacac
      361 gaaccccatg tacacgccgc gcgagctcag gcaccagctc gcggactcgg gcgcgcgcgc
      421 gatcctgatc ctcgagaact tcgcgcacac gctcgagcag gtcatcgacg acgtggacct
      481 cgacaccgtc atcgtcacgt ccgtcggtga cctgctcggt ttcccgaaat cggtgctcgt
      541 cgacttcgcg ctccggcacc tccgcaagca ggtgcccgac tggagcctgc cgggcgcgat
      601 cggtttcggg gacgcgttga accaggggcg gttcgagaac gcgacgcccg ttgcgctggg
      661 ccacgaagac gtcgcattcc tgcagtacac gggcggcacg accggcgtat cgaagggggc
      721 ggtgctcacg caccggaaca tggtcgcgaa cgtgctgcag gccgaggcgt ggatcaagcc
      781 ggcgctcaag cctggcgagc ccatggtctc gatcacggca ctgccgctgt accacatctt
      841 cgcgctgacc gcgaacagct tcacgatgct gaagctcggc gcgcacaacg tcatgatccc
      901 gaacccgcgg gacttcccgg ggttcgtgcg cgaactgtcg aaacatcgct tcacgttctt
      961 cgcgggcgtc aacacgctgt tcaacgcgct gctgcacacg cccgggttcg accggctcga
     1021 cttctccacg ttgcggatca gcctcggcgg cggcatggcc gtgcagcgcg cggtggcgga
     1081 gaactggaag cgcgtgaccg gcaacgtgct cacgcaggcg tggggcctga cggaaacgtc
     1141 accggccgcg tgcatcaacc gtccgggcga cgacttcaac ggctcgatcg gcctgccgat
     1201 cccgtcgacg ctggtcacga tccgggacga cgccggcaac gtgctgccgc agggacagtc
     1261 gggagaaatc tgcgtcgagg ggccgcaggt catgcgcggg tactggaacc ggcccgacga
     1321 gaccgagaat gtcatgctgc cgggccaggt cctgcgcacg ggcgacgtcg gccacatgga
     1381 cccggacggc ttcgtctaca tcgaggaccg caagaaggac atgatcctgg tgtccggctt
     1441 caacgtgtac ccgaacgaag tcgagggcgt cgcggtcacg cacgcgggcg tgctcgaggt
     1501 cgccgcggtc gcgcaggcgg acgagcgcgc cggcgaagtc gtcgcgctgt tcgtcgtgcg
     1561 caaagacccg aagctcggcg agcaggagct gatcgatcac tgccgcgcct cgctgaccgg
     1621 ctacaaggtg ccgaagcgcg tctatttccg ggacgaactg cccaagacca acgtcggcaa
     1681 gatcctgcgc cgcgagctca aggaccagtt gaagacgtcg tgaggcgcgt tgcgcaccgc
     1741 ggcgtgacgg ggatgagcgc catcacgcac aatcgcgggc cctggggctg gcccgaagga
     1801 tcgtgatggc cgattcggtg ctctcgttga tcggcaacac gccgctcgtg cgggtccgct
     1861 cgttcgagac cgggccatgc gagctgtacc tcaagctcga aagccagaat cccggcgggt
     1921 cgatcaagga ccgcatcggc ctctcgatga tcgaggcggc cgagcgcagc ggccggctgc
     1981 agccgggggc caccatcgtg gaggcgacgg ccggcaacac cggactcggg ctcgcgctgg
     2041 tcgcggccca gaagggctac cggctggtgc tggtggtgcc ggacaagatg tctcgcgaga
     2101 agatcctgca cctggcggca ctcggcgccg acgtgcgact gacgcgctcc gacgtcggca
     2161 agggccatcc acagtactac caggacatgg cgcagcgcat cgcggcggag atgccgggtg
     2221 cgtactacat caaccagttc ggcaacccgg ccaatccgct cgcgcacgag acgacgaccg
     2281 ggcccgagat cctgcgccag atggacgggc gggtcgacgc gatcgtctgc ggcgtgggct
     2341 cgagcggcac cgtcaccggg ctcagtcacg tcttcgcacg cgagtcgccc ggtacgcggt
     2401 tcgtgctggc agatccggtc ggctcggtgc tcgcctccta tgtcaacacg ggcgtgctcg
     2461 gccaggccgg ctcgtgggcg gtcgaaggca tcggcgagga tttcattccg gcgatcgccg
     2521 acctcagccg tgtctcggtc gcgtactcga ttcccgacgc ggagagcatg gcgacggtgc
     2581 gcgcgctgct gcgccaggaa gccatcttcg gcggttcgtc gacgggcacg ctgctggcgg
     2641 cggcactgcg ctactgccgc gagcagacgc agccgcagcg ggtggtgacc ttcgtctgcg
     2701 acggcggcgc caagtacctg tcgaaggcct acaacgacgc ctggatgtac gagcgcggat
     2761 tcctcgagcg cgaacagtcc ggcgaccttc gcgacctgat cgttcgtcgc cacgacgcgg
     2821 ggctcacgat caccgtgcag cctggcgact cgctgctgac ggcctacaac cggatgcgca
     2881 gcgccgacgt ctcgcagttg ccggtcgtgg aggaagacgg ccgcatcgtc ggcatcctcg
     2941 acgagttcga cctgctgcag gtcgtgcgac gcgccgacgc cgcggaacgc ttccagcagc
     3001 ccgtgcggac cgcgatggaa acgcggctcg agaccctcga gcccgacgcc gggctcgacg
     3061 cgctgttcgc cgtgctcgac gccggccacg tcgcgctcat cgtcgagcgc ggcacgttcc
     3121 tcgggctcgt cacgcgcatc gacctgctca acgccctgcg actgagaacg ctatgaccga
     3181 tcatgaccga aacgcacggc ctcgcttcgc cacccgcgcg atccacgcgg gccaggaacc
     3241 cgacccggcc accggcgcgg tgatggtgcc gatctacgcc acctcgacgt acgtgcagtc
     3301 gagtcctggc gtccacaagg gcttcgacta cgggcgcagc cacaacccca cgaggttcgc
     3361 gttcgaacgc tgcgtggccg atctcgagaa cggcagccag gcctttgcgt tcgcctcggg
     3421 cctggccgcg atcggcacgc tgctcgaatg cctcgatgcg ggctcgcaca tcgtcaccac
     3481 cgacgatctc tacggcggaa cggtccgcct gctcgagcgg gtgcggcagc gctcgatgaa
     3541 cctgtcctcg acacgggtcg acttcaccga cctgtccgcc gtcgagcgtg cgatccggcc
     3601 ggagacgcgt ctgctgtggg tcgaaagccc tacgaacccc acgctcaagc tcgccgacct
     3661 cgaggcggtc gcggcgctgg cgcggcgtcg cggattgctg gccgtcgcgg acaacacgtt
     3721 cgcgagcccc tgggtgcagc gtccgctcga cctcggcttc gatgccgtcg tgcattcgac
     3781 gaccaagtac ctgaacggtc actccgacat gatcggcggc gtggccgtgg tggggcagaa
     3841 cgccgaactg cgtgaaaggc tgggcttcct gcagaatgcg gtcggtggaa tctcgggccc
     3901 gttcgacagc ttcctcgcgc tgcgcgggct caagacactc gcggtgcgca tgcagcgaca
     3961 ctgcgagaac gcgctcgcga tcgcgcagtg gctcgagcga caggggaagg tgcggcgcgt
     4021 gctctacccg ggactgccga gccatccgca gcacgcgctg gcgttgcgcc agacgccgcg
     4081 gttcggcggc atgctctcgg tcgagttcga caccgacctg gccggcgcac gtcgcctgct
     4141 cgagcgctgc cggatctttg cgctcgccga gagcctcggg ggcgtcgaga gcctgatcga
     4201 gcatccggca ttgatgaccc attcgcagct cgcacccgag cggcgccagt cgctcgggat
     4261 cggtgacgga ctgatccgga tctcggcggg catcgaggac gtcgaggacc tgatcggcga
     4321 cctcgagcag gccatgcggg cgctcggcta ggccggggtt accggcagcg aggacttcga
     4381 gtcgccggcg gcggcccccg gccgaggact cgtgccgcct cgcagctcag gacctgcgtg
     4441 cggaggcctt cagcgcgccg ctcgcgacat agtgtgcggc gacgtcgtcc ccggcctgcg
     4501 cggcacggcg gatgccctcg acgatcatgc gctcggcctt gtccgtgccg acccagttca
     4561 cgatcgtgac ccacttgccc ttctcgaggt ccttgtagtg ctggaagaag tgagcgatct
     4621 ggtcggtcag cgtcgccggc aggtcgcgat acgacttcac gccggaatag aacgggtgca
     4681 gcttgtcgac cgggacggcg atgatcttct cgtcgtcgcc ggcttcgtcc ttcatcagca
     4741 gcgctcccac cggccgcacg cggacgatcg cgccggcgac gacggggctc ggcacgacca
     4801 cgatcacgtc cacgggatcg ccgtcgcccg acagcgtgtg cgggatgaac ccgtagttgc
     4861 ccggatagaa catcgccgtg ttcaggaagc ggtcgacgcg cagcgcgccg gattccttgt
     4921 cgagctcgta cttgaccggc acgccgccct gcggaatctc gatcaggacg ttgacgtctc
     4981 gcggcggact cgggccgatg ctgatcttgg aaatgtccat gggaggaagt ggctagtggc
     5041 cagtggtcag tggttggtgc aagaaagtgt agcgtttggg ggctcgccct gttgataagg
     5101 cggcgcagcc gctccgacta gccactagcc actagcca
//
LOCUS       DASPFR010000331         6049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_707975, whole genome shotgun sequence.
ACCESSION   DASPFR010000331 DASPFR010000000
VERSION     DASPFR010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707975"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VD737_08750"
     CDS             complement(<1..302)
                     /locus_tag="VD737_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10690.1"
                     /translation="MAARKKARRPALRSKKASKAKSTSKRRVVAKTRATARKAKAPVR
                     KTAKRKVAKPRKVAKARKVAKPRKVTSSRVSAVRAAARKAPGRARAAVKRAVRTLPP"
     gene            complement(499..3249)
                     /locus_tag="VD737_08755"
     CDS             complement(499..3249)
                     /locus_tag="VD737_08755"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HXW10691.1"
                     /translation="MPRKDISFPAKDIALRDDVHTLGALVGEMLRDQGGEHLFQEVEH
                     DRQLAIRRRDEDCDASVELVVRAEARTPEQAADLIRAFATWFQMVNMAEKVHRVRRRR
                     DYLNDSSRPQPGGLTECFQRLKTLGYSLAQTVDLLGRLSIEPVFTAHPTESTRRTLLR
                     QQQRIATLLLSRLDPSMTPAERRITLERVRTELTTGWQTAGNSRERLTVADEREHVLF
                     FLVEVIYDIIPLFYEAIEESLSVVYGSDAHRVAVPEMLRFGSWVGGDMDGQPDVHAKT
                     IRESVARHHALIVNRYYLEAQRLAEKLSQSERRTPAIPQAIRDRIEHYKSVVPAARAS
                     TPSGHDEMPYRVLLGQIAERLRATYDHRSGQYERVEQLIDDLNLIARSLEANRGRWAG
                     LFMVRRFLRRVRTFGFHLATLDVRQNADVHRQVVGRALGIPDWEQLSPAQRTERLREA
                     LAKDESPPDTLEAAGKRALWVFEAIEYCRHRYGRAAVGSYVVSMARDADDILSVLLLA
                     RWAGFAETRTDTLPLDVAPLFESIESLEAAGEILGRLLGDPIYRTHLRGRGDTQIVML
                     GYSDSAKAAGIAGSRWALRKAQLAMLQACERAGVGLLVFHGRGGSVSRGGSRTEAVVG
                     GLPTAVRSGRLRITEQGETVNDRYGLRPIALRTFEQGLNTLSLAVAGDRKETVREGWS
                     EAMETCAQASVRRYRAFVHDDPRFFEFFQAATPIDVIERMQIGSRPLARGSGGGVDAI
                     RAVPWHFAWSQSRHMLPGWFGAGTGLAAMIERFGRETADAMYQGWPFFAGLVDDVEMR
                     LARADMGIAAHYERLYEGNVERYAVPIRQEYELTRTLVLQLKGCARLLDSEPTLQRSI
                     WLRNPYVDPIHLMQIQLLRRWRGAGSPSDKGRGPDDPDRHLFQALVASVNGIAQGLQG
                     TG"
     gene            3476..6037
                     /locus_tag="VD737_08760"
     CDS             3476..6037
                     /locus_tag="VD737_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXW10692.1"
                     /translation="MKIGKSRLFALAAAHGASLLTVPAVLQAQAQAQAPSGQLEEIIV
                     TARQREERIEDVPVSITAFTASEIRSAGIERPTDFIALTPGVSQVQTAEAGDLQVNIR
                     GINTGRDAETNFALVIDGVLQTNPNALNQELSGVTQIEVLKGPQGALYGRNALAGALI
                     LTTRKPTDEWASEVTAGYGSDNSYKGSVYFGGPLGEALRGAVSAYTRHTDGQWENLKN
                     DCDDCVDKFEETGFAGRLLFELGGGEMDFKAKYSELESGAINFNGSVALPDAAAFLGI
                     PALYEDPNDHNFRYINNINPQNEQENLNLSLKGDWDVGFATLTGYVAWNDQTNFFLTD
                     GASDAFFLYAFDDTCRVTNDSNLTDPGYEAPFFGVPSNLWFTPEGGGAAGFLPPYGPS
                     TCGGYQYQQRDQEDLSVELRLTSPGDQRLRWVGGVYFADIDRHVVVSQGSDQQQGFRT
                     TGFVPASGPNPTDLLYDDDFSSKVYAVFGQVAYDVVDNLELALALRYDSEDREVSNNV
                     PTCADGGDRCFAQTPNFGAGWGFLVPLPYYINPAYQTDPALATSGIPNRSETFEQLQP
                     KVSLNWNLTDGFALFASYGYGFRSGGFNSSGSSATADVWYGALTLDDGTQNLSEVSDQ
                     YDKEVSKAAELGFKSYFLDRSVSLNAALYHTTVEDMQFFNFFAGPFGLLRVVTNIDEV
                     TIQGAEIDARWQINEMFTVFAGYGITDGNIDKYSGRPYTKGNEVPYAPEYTGNIGAEA
                     TFPVTESLELIARIDGTFVGETWFHPVQEERLPNLFGYFGFGQGEFSKQVRDPYAVFN
                     ARLTLQGANWGVTAWGRNISDEDYLAEIIPAPEFGGSFVHDAPGDSYGVDLSYRF"
BASE COUNT          968 a         2096 c         2026 g          959 t
ORIGIN      
        1 ggcggcagcg tgcgcacggc gcgcttcacc gcggcgcgtg cacgccccgg ggccttgcgg
       61 gccgcggcgc gaacggcgga aacgcggctg ctggtgacct tgcggggctt ggcgaccttg
      121 cgcgccttgg caaccttgcg cggcttcgcg accttgcgct tcgccgtctt gcgcacggga
      181 gccttcgctt tgcgcgcggt cgcgcgggtc ttggcaacga cccggcgctt gctggtgctc
      241 ttcgccttcg atgccttctt gctgcgtagc gccggtcggc gcgccttctt cctggctgcc
      301 attgcgtctt tctccctcga tttggttggc gtagtgagtg acggggctcg cgtggcggcg
      361 tcgttgccgt gagcaaccca cgtcgtcggc tgtatccgtc gtgcacggcc gacgctagac
      421 cacgcgccgt atcgcgtcaa cacggagaaa cacagccgcc gcttgggacg cgtgcacggc
      481 cacgcgagca cgcagcgctc aaccggtgcc ctgcaggccc tgcgcgatgc cgttgacgga
      541 ggcgaccagc gcctggaaca ggtgccgatc cgggtcgtcg gggccccggc ccttgtcgct
      601 cggggagcct gccccgcgcc agcgccgaag cagttggatc tgcatcaggt ggatcgggtc
      661 gacgtagggg ttgcgaagcc agatcgagcg ctgcagcgtc ggctcggagt cgagcagccg
      721 cgcgcacccc ttcagctgca gcaccagcgt gcgcgtgagc tcgtattcct ggcgtatcgg
      781 cacggcatac cgttccacgt tgccttcgta cagccgttcg tagtgcgcgg cgatgcccat
      841 gtcggcgcgg gcgagccgca tttccacgtc gtccaccagg ccggcgaaaa acggccagcc
      901 ctggtacatc gcgtcggccg tctcacgccc gaaccgctcg atcatcgccg cgagcccggt
      961 gcccgcacca aaccaacccg gcagcatgtg ccggctctgc gaccacgcga aatgccaggg
     1021 caccgcccgg atggcgtcga cgcccccgcc gctgccgcgc gcgagcgggc gggagccgat
     1081 ctgcatgcgc tcgatcacgt cgatcggggt ggccgcctgg aagaactcga agaagcgcgg
     1141 atcgtcgtgc acgaaagccc ggtagcggcg cacgctcgcc tgcgcgcagg tttccatcgc
     1201 ctcgctccag ccctcacgga cggtctcctt gcggtcgccg gcgaccgcga gcgagagcgt
     1261 gttgagtccc tgctcgaacg tccgcaacgc gatcggccga aggccgtacc ggtcgttgac
     1321 cgtctcaccc tgctcggtga tgcgcaggcg gccactgcgc acggcggtcg gcaggccgcc
     1381 gaccaccgcc tcggtgcggc tgccaccgcg gctcaccgaa ccgccgcggc cgtggaagac
     1441 gagcaggccg acgccggcac gctcgcaggc ctggagcatc gcgagctgcg ccttgcgcag
     1501 ggcccagcgc gagcctgcaa tgccggccgc cttcgcgctg tcggaatagc ccagcatgac
     1561 gatctgcgtg tcaccacgac cgcgcaggtg cgtccggtag atcgggtcgc ccagcagtcg
     1621 gccgaggatc tcgcccgcgg cctccagcga ctcgatcgac tcgaacagcg gtgcgacgtc
     1681 gagcggcagc gtgtcggtcc gggtctcggc gaaacccgcc catcgcgcca gcaggagcac
     1741 cgacaggatg tcgtcggcgt cgcgagccat gctgaccacg tacgacccga ccgcggcgcg
     1801 cccgtaacga tgtcggcagt attcgatcgc ctcgaacacc cacagcgcgc gcttgccggc
     1861 ggcttcgagc gtgtcagggg gcgactcgtc cttggcgagc gcttcgcgca agcgctccgt
     1921 gcgctgcgcc gggctgagct gctcccagtc cgggatgccc agcgcgcgcc cgacgacctg
     1981 ccggtgcacg tcggcgttct gccgcacgtc gagcgtcgcc aggtggaacc cgaacgtgcg
     2041 gacgcgccgc aggaatcgtc gcaccatgaa gagcccggcc cagcgtccgc gattggcttc
     2101 gaggctgcgc gcgatcaggt tcaggtcgtc gatcagctgc tcgacgcgct cgtactggcc
     2161 cgagcggtgg tcgtacgtcg cgcgcaggcg ttcggcgatc tggcccagca ggacccggta
     2221 cggcatctcg tcgtggcccg aaggggtgct cgcacgcgcg gcggggacca ccgacttgta
     2281 gtgctcgatc cggtcgcgga tcgcctgcgg gatggcagga gtccgacgct cgctctggct
     2341 cagcttctcg gccagtcgct gagcctcgag gtaataacgg ttcacgatca gcgcgtggtg
     2401 gcgggcgacg ctctcgcgga tcgtcttggc atgcacgtcg ggttggccgt ccatgtcgcc
     2461 gccgacccag gacccgaagc gcagcatctc cggcacggcg acgcgatgcg cgtcggaccc
     2521 gtaaacgacc gacaacgact cctcgatggc ctcgtagaac aacgggatga tgtcgtagat
     2581 gacttcgacc aggaagaaca gcacgtgctc gcgctcgtcg gcgaccgtga gtcgttcgcg
     2641 ggagttgccg gcggtctgcc agccggtcgt cagctcggtt cgcacccgct cgagcgtgat
     2701 gcggcgttcg gcgggcgtca tcgacgggtc gagccggctc agcagcaacg tggcgatgcg
     2761 ctgctgctgg cgcagcagcg tgcggcgcgt cgactccgtg gggtgcgccg tgaacacggg
     2821 ctcgatactg agccgcccga gcaggtcgac cgtctgcgcg agcgaatagc cgagcgtctt
     2881 tagccgctgg aagcactcgg tcaggccgcc cggttgcggc cggctgctgt cattcaggta
     2941 atcgcgccgg cggcgcacgc gatgcacctt ctcggccatg ttgaccatct ggaaccaggt
     3001 cgcgaacgcg cggatcaggt cggcggcctg ttccggcgtg cgggcctctg cgcggacgac
     3061 cagctcgacg ctcgcatcgc agtcctcgtc gcggcggcgg atcgccagct gccggtcgtg
     3121 ctcgacctcc tggaacaggt gctcgccgcc ctggtcacgc agcatctcgc ccacgagcgc
     3181 gccaagcgtg tgcacgtcgt cgcgaagcgc gatgtccttc gccggaaacg aaatgtcctt
     3241 gcgtggcatg gccgcggtgc gcgtctagga ctggtgggat gtggccgcgg cgggacggga
     3301 gcgctgtgcg agcgtggacg cgcatgctac caaagctccg cgtcgagtct tgttgcgctg
     3361 cgacaacatt gcgtgccggt tgtattaaac atagaacttt gagactttat tggcgggtcg
     3421 accgagcgcg tgcacagacg gcgcgcgccg tacaagagcc acgcaagggg gacgcatgaa
     3481 gatcggaaag tcgcgcctgt ttgccctggc tgccgcgcac ggcgcctcgt tgctcaccgt
     3541 gccggccgtg cttcaggcgc aggcccaggc ccaggcgccg agcggccagc tcgaggagat
     3601 catcgtcacg gcacgacagc gcgaggagcg catcgaggac gttccggtgt cgatcacggc
     3661 gttcacggcg agcgaaatcc gcagcgccgg catcgagcgc cccaccgact tcatcgccct
     3721 cacgcccggg gtctcgcagg tgcagaccgc cgaggcgggc gacctgcagg tcaacatccg
     3781 cggcatcaac accgggcgcg atgccgagac caatttcgcg ctggtgatcg acggcgtcct
     3841 gcagaccaat cccaacgcgc tcaaccagga gctctccggc gtcacgcaga tcgaggtgct
     3901 caagggcccg cagggcgcgc tctatggccg caatgcgctc gcgggcgcgc tgatcttgac
     3961 gacccgcaag ccaaccgacg aatgggcctc ggaggtcacc gccggctacg gctcggacaa
     4021 ctcgtacaag ggcagcgtct acttcggcgg gccgctgggt gaggcgctgc gcggcgcggt
     4081 ctcggcctac acccgccaca cggacggcca gtgggagaac ctcaagaacg actgcgacga
     4141 ctgcgtcgac aagttcgagg aaacgggctt cgcgggccgg ctgctgttcg agctcggcgg
     4201 cggcgaaatg gacttcaagg ccaagtattc cgagctcgag tccggcgcga tcaacttcaa
     4261 cggctccgtc gcgctgcctg acgccgcggc gttcctgggc atcccggcgc tgtacgagga
     4321 tcccaacgac cacaacttcc gctacatcaa caacatcaat ccgcagaacg agcaggagaa
     4381 cctcaacctc tctctgaagg gcgactggga cgtcggcttc gccacgctga ccgggtacgt
     4441 ggcctggaac gaccagacca acttcttcct gaccgacggc gcgagcgacg cgttcttcct
     4501 gtacgcgttc gacgacacct gccgggtcac caatgactcc aacttgaccg acccgggcta
     4561 cgaggcgccc ttcttcggcg tgccctccaa cctgtggttc acgccggaag gcggtggcgc
     4621 cgcgggcttc ctgccccctt acggcccgtc gacctgcggt ggctaccagt atcagcagcg
     4681 cgaccaggaa gacctgagcg tggagctgcg gctgacctcg cccggcgacc agcggctgcg
     4741 atgggtgggc ggggtctatt tcgcggacat cgatcgtcac gtggtcgtct cgcaaggctc
     4801 cgaccagcag cagggcttcc gcacgaccgg cttcgtgccg gcgagcggcc ccaaccccac
     4861 ggacctgctc tacgacgacg acttcagcag caaggtgtac gcggtgttcg gccaggtggc
     4921 gtacgacgtg gtcgacaacc tcgaactcgc gctcgccctg cggtacgaca gcgaggaccg
     4981 cgaggtcagc aacaacgtgc cgacctgtgc cgacggcggc gaccgctgct tcgcgcagac
     5041 gccgaacttc ggcgcgggct ggggattcct cgtgccgctg ccctactaca tcaaccccgc
     5101 gtaccagacc gacccggcgc tcgcgacgtc gggcatcccc aatcgcagcg aaaccttcga
     5161 gcagctgcag ccgaaggtgt cgctcaactg gaacctgacc gacgggttcg cgctgttcgc
     5221 ctcctacggt tacggcttcc gcagcggtgg cttcaactcc tcgggcagct ctgccacggc
     5281 ggacgtctgg tacggcgcgc tcaccctcga cgacggcacg cagaacctga gcgaggtgag
     5341 cgaccagtac gacaaggaag tctccaaggc ggcggaactc ggcttcaagt cgtacttcct
     5401 cgaccgatcc gtgagcctga acgccgcgct gtaccacacg accgtcgagg acatgcagtt
     5461 cttcaacttc ttcgcggggc cgttcgggct gctgcgcgtc gtcaccaaca tcgacgaggt
     5521 gacgatccag ggcgcggaga tcgacgcgcg ctggcagatc aacgagatgt tcacggtgtt
     5581 cgcgggttac ggcatcaccg atggcaacat cgacaagtac agcggccggc cgtacacgaa
     5641 gggcaacgaa gtgccgtacg cgccggaata caccggcaat atcggcgccg aggccacctt
     5701 ccccgtcacc gaatcgctgg aactgattgc gcgcatcgac ggcacgttcg tcggcgaaac
     5761 ctggttccac ccggtgcagg aagagcgcct gcccaacctg ttcggctact tcggcttcgg
     5821 ccagggcgag ttctcgaagc aggtgcgtga cccgtacgcc gtcttcaacg cccggctcac
     5881 gctgcagggc gcgaactggg gcgtgacggc ctggggccgc aacatcagcg acgaggacta
     5941 cctcgccgag atcatccccg caccggaatt cggcggctcg ttcgtgcacg acgcgccggg
     6001 cgattcctac ggcgtggacc tgagctaccg cttctgaccc tgtcgcccc
//
LOCUS       DASPFR010000332         3107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_344133, whole genome shotgun sequence.
ACCESSION   DASPFR010000332 DASPFR010000000
VERSION     DASPFR010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344133"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VD737_08765"
     CDS             complement(<1..260)
                     /locus_tag="VD737_08765"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXW10693.1"
                     /translation="MHRVPEPELMDEAEQARAYALADFAEPNERFVGYFEAYCAGLST
                     GAVLDLGCGPGDIVLRLATRHPDLVVHGIDGSAAMLHFASERL"
     gene            complement(313..585)
                     /locus_tag="VD737_08770"
     CDS             complement(313..585)
                     /locus_tag="VD737_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10694.1"
                     /translation="MPAAIDRKAAAGLSLALVAIFALGACNSPPPEEPDEEEARALGR
                     DTDETVFDDMVQTQDKARAVEDLTLGRKDELDQALDASEGDAPVEE"
     gene            645..1448
                     /locus_tag="VD737_08775"
     CDS             645..1448
                     /locus_tag="VD737_08775"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXW10695.1"
                     /translation="MDAFSDRVVTVTGASEGIGRAFCLALAPQRPRLVLAARNAERLE
                     SLAGECRRLGAQALVAPTDVTDENACRTLIETAVTHFGGIDVLVNNAGGTMWTRLDAI
                     ADLSIFERLMRLNYLGSVYPTFHALPHLKRAQGRIVAVASMAGLIGVPTRTGYSAAKH
                     AVVGFFDSLRVELADEGVSVTVVCPDFVVSEIHKRALGPDGRPLGDNPMAHSRIMSAE
                     ECARLMIGAIERRDRLLVTSARGRLARWLKLVAPARIDALAAQAIREKK"
     gene            complement(1565..2077)
                     /locus_tag="VD737_08780"
     CDS             complement(1565..2077)
                     /locus_tag="VD737_08780"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXW10696.1"
                     /translation="MTERYVVFSHGKDSEPWGNKIAAMAEIAREEGFHVESVDYRGIE
                     DPQARVTRLLAFCKDLRGSLVLVGSSMGGYVSVAGSSLLQARGMFLLAPALYMPGYEK
                     ISPRPAHCPTTIIHGWRDDVIPVDNSVRFAREQKATLHVVDSDHRLLDQIRAINHYFA
                     YFLFQIDEFL"
     gene            complement(2148..2543)
                     /locus_tag="VD737_08785"
     CDS             complement(2148..2543)
                     /locus_tag="VD737_08785"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HXW10697.1"
                     /translation="MHMVRNTGIAPAAAAERRAAAIIAVVERIPRGCVTTYGSVATRA
                     GLPRQARLVGKVLASLPPRSPVPWQRVVAAGGRIAFPEGSPSRARQLRLLRAEGVDAT
                     RGRVDLARHGWGAPGGRDLDQWLWSRDDG"
     gene            2628..>3107
                     /locus_tag="VD737_08790"
     CDS             2628..>3107
                     /locus_tag="VD737_08790"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HXW10698.1"
                     /translation="MTTIGWRFAIDRGGTFTDVVGVAPDGALHLAKVLSRDPAHPGDP
                     ALRGLRSVLDAHGEQHGRTVASVRLGTTVATNALLERTGAATLLVTSQGYGDALAIGY
                     QERPDIFARAIRRPAPLYQAVAEVPERIDAAGAVLTPLDDAALHRALAAARRDGMQAV
                     "
BASE COUNT          464 a         1081 c         1089 g          473 t
ORIGIN      
        1 aggcgctcgc tcgcgaagtg cagcatcgcg gccgaaccgt ctataccgtg cacgacgagg
       61 tccggatgac gcgtcgcgag ccgcagcacg atgtcgccgg gcccgcagcc gaggtcgagc
      121 acggcccccg tcgacagccc ggcgcagtac gcctcgaaat acccgacgaa ccgctcattg
      181 ggctcggcga agtcagcgag cgcgtacgca cgggcctgct cggcctcgtc catcagttcg
      241 ggttcgggga cgcggtgcaa gggaagtggc tagtggccag tggtcagtcg gacgggcgcc
      301 gcttcgagcg catcattctt cgaccggcgc gtcgccttcg gaggcgtcga gcgcctggtc
      361 gagctcgtcc ttgcgaccga gcgtcaggtc ttcgacggcg cgcgccttgt cctgggtctg
      421 gaccatgtcg tcgaacacgg tttcgtcggt gtcgcgaccg agtgcgcgcg cctcctcctc
      481 gtccggttcc tcgggtggtg gggagttgca ggcgccgagc gcgaagattg cgacgagcgc
      541 gagcgataat ccggcggcag ccttcctgtc gatggcagcg ggcatgcgtc atcccccgag
      601 tcgttgaccc ggggcgcgat tctaacgcaa agggagtcct ggtcatggac gcattctcgg
      661 accgagtcgt gaccgtcacc ggcgcctcgg aagggatcgg acgcgcgttc tgcctggccc
      721 tcgcgccgca acgtccgcgc ctggtgctgg cggcacgcaa cgcggagcgg ctcgagtcac
      781 tggccggcga gtgccgtcgg ctcggcgcac aggcgctggt cgcgccgacg gacgtcacgg
      841 acgagaacgc gtgccgcacg ctgatcgaaa ccgcggtgac gcacttcggc ggcatcgacg
      901 tgctcgtcaa caacgccggc ggcacgatgt ggacgcggct cgacgcgatc gcagacctgt
      961 cgatcttcga gcgcctgatg cggctcaact acctcggcag cgtgtacccg acgtttcatg
     1021 cgttgccgca cctcaagcgc gcgcagggtc gcatcgtcgc ggtcgcgagc atggcggggc
     1081 tgatcggcgt gccgacccgg accggctatt cggccgccaa gcatgccgtg gtgggcttct
     1141 tcgattcgct gcgcgtcgaa ctcgcggacg agggcgtgtc cgtgactgtc gtgtgccccg
     1201 acttcgtcgt gtccgagatc cacaaacgcg cgctcggtcc cgacggccgg ccgctcggcg
     1261 acaatccgat ggcccactcg cggatcatgt cggccgagga gtgtgcgcgc ctgatgatcg
     1321 gcgcgatcga gcgccgcgac cggctgctcg tgacgtccgc ccgtggccgc ctggcgcgct
     1381 ggctcaagct cgtcgccccg gcgcggatcg acgcgctggc ggctcaggcc atacgggaga
     1441 agaagtaaag cagcggttag cggttagtga tcagcggtta gtcggaggcg acgtcgacgt
     1501 gattggcggg cctcttgctg accgctaacc gctaaccgct aaccgctaac cgctaaccgc
     1561 ttcctcacag gaactcatcg atctggaaca ggaagtacgc gaagtaatgg ttgatcgcgc
     1621 ggatctggtc gagcagccgg tggtcggagt cgacgacgtg cagcgtcgct ttctgctcgc
     1681 gcgcgaagcg gacgctgttg tcgacgggga tcacgtcgtc gcgccagccg tggatgatcg
     1741 tcgtcggaca gtgtgccggg cgcgggctga tcttctcgta gcccggcatg tacagcgcgg
     1801 gcgcgagcag gaacatcccg cgcgcctgca gcagcgacga gccagccacc gacacgtaac
     1861 cgcccatgct cgaaccgacg agcacgagcg acccgcgcag gtccttgcag aaggcgagca
     1921 ggcgcgtcac gcgcgcctgc ggatcctcga tgccacggta gtcgaccgac tcgacgtgga
     1981 agccttcctc gcgggcgatc tcggccatgg ccgcgatctt gttgccccac ggctcgctgt
     2041 ccttgccgtg cgaaaaaacc acgtagcgtt cggtcatcgg gcggccactc caggcgtgtg
     2101 cccacgcatc ttagcctgcc gccccgccgt cgatggcgac gctcgtctca tccgtcgtcg
     2161 cgcgaccaga gccactggtc gagatcgcgc ccgcccggcg cgccccagcc gtgacgggcg
     2221 aggtccacgc gaccgcgcgt cgcgtcgacg ccttcggcac gcagcagccg aagctgtcgc
     2281 gcacgggacg gcgacccttc ggggaaggcg atgcggccgc cggccgccac gactcgctgc
     2341 caggggaccg ggctgcgggg cggcagcgac gcgagcacct tgcccaccag ccgggcctgg
     2401 cgcggcaggc cggcgcgcgt ggcgacgctg ccgtaggtcg tcacgcatcc gcgcggaatg
     2461 cgctcgacca cggcgatgat tgcggccgcg cgtcgttcgg ccgccgcggc cggcgcgatc
     2521 ccggtgttcc tgaccatgtg catgagctct ccgccagccg tgaataacca atctgccaga
     2581 atggcgcgct tgcgtgaacg ccgaccgatg acccgcgggc ctgccacatg accacgatcg
     2641 gatggcgatt cgcgatcgat cgcggcggaa cgttcaccga cgtcgtgggg gtcgcgccgg
     2701 acggcgcgct gcacctggcg aaggtgctgt cgcgcgatcc tgcgcatccg ggcgatccgg
     2761 cgctgcgcgg gctccgcagc gtgctcgacg cacacggcga gcagcacggc cggacggtcg
     2821 cctccgtacg cctcggcacg accgtcgcca ccaatgcgct gctcgaacgc accggggccg
     2881 ccacgctgct ggtgacgtcg cagggctacg gcgacgcgct cgcgatcggc taccaggaac
     2941 ggccagacat cttcgcgcgc gcgatccggc gtcccgcacc gctgtaccag gccgtcgccg
     3001 aggtaccgga acggatcgac gcggcgggcg ccgtgctcac gccgctcgac gacgcggcgt
     3061 tgcatcgcgc gctggccgcc gcccggcgcg acggcatgca ggcggtc
//
LOCUS       DASPFR010000333         4207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_221682, whole genome shotgun sequence.
ACCESSION   DASPFR010000333 DASPFR010000000
VERSION     DASPFR010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221682"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4207
                     /locus_tag="VD737_08795"
     CDS             <1..>4207
                     /locus_tag="VD737_08795"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10699.1"
                     /translation="IDGGPDDDVLIGTEGNDRIDGMSGSDTIYGRSGNDLLWGDSTVG
                     EEAFSTNTIDVIFGGRGNDDLIGGPGINDLYAWSKDPLPDGDTEFGVFVHPSAPDGPL
                     FDDSQGGTLVLEDTGIDRMLGSRNADRLFGGTKVSFLFGNGGDDQLFTVTGELFETAD
                     GGLDVDTWKQFAQASGQAIYVAGTETDDVITVDFVTEPGPLAGRHLITRLTNNNGVFT
                     FAAQDEFQFLLDAASDPDNVDTVTPLEGLQQRGGQQEDPLNPDLTIETPGTEPQSAGT
                     EQGGTLEGLVPQGGGVEVIIIDALGGDDLITVGPTVQHSVWIDAGDGDDVVKIQGGEV
                     ILSDKTELVQPSNPVPVEGDEARNDSAASAFYLSQDPFAAHPANEVALPGSTTFSGLN
                     IDNPDDEDWYKFSIADDDGDGLVGEGALLTLGSVSDNDGLEIALFTTDGTTMLESGLL
                     QLSRDFTDVGGSPANTTASAFELPAVQSLGRASGLTIHDPQDADVFRFELERAGVSGD
                     RINLLKNSIDDDLVIELLDAGGDVVVEGEEFAPLVDAVSLEGLAAGTYYIRVSTEGAL
                     ARYDLIFQVPGLRQTERSVTPAPNDSDETPFDLGAHSNFPTLTGTSVGLGEEEWYAFE
                     MLRDGSPADEIRIESSAFVQLRLFQRDQDGELDDLDLDPVAAEAGTPGVISLATLDKG
                     DYLLRVSGALGPTSYELFPGDRTTERTIVEGVDGTGELTRTEIRTLREFGTSNLDLAG
                     AAETFVDLSGLNANQTYLLRVTSPNRIPTIYDLTFDFSSAGSPADLSVEKELVIGSST
                     PSVGEPVKIEVKVTNDGDNEAINVSLTDLLPAWLTPTASQGIYDPATGLWQVGLLFTG
                     ASATLTFEGVVPMGQGGTPLPLEEPTATGTVALATDQPFEVDLSVKANTDRRDVIIGG
                     PGNDALQGGPGSTWVFGMAGNDVLTGGYDRQSPDLLFGGEGDDAFQLLPDQLPFIKGT
                     TETYLPTLVDRFDGGPGNDFVLFQGGDLDNLNRPVDDWVAIRWDRFQQRYEFTAVPFD
                     TANQVFAVEQEVVNATKLAPLDGFDGEVNFRLRVPIPGMPDQGFVEMSVDIQDAGNIS
                     ELADQLQAALVAEFGVDENGNPVVIVEFPDGILRLRARVQGLELRAEADDLMVTALGF
                     EPLTAGSPIFLQHYAFFQALSVEKIVIDTRGGDDIVHADPEYFFPNVPTTEWGLAEGS
                     FEEGAKIPLEIRLGDGNDRGFGSSQDDTIFGGEGADIIFGYLGNDLLYGGSGRDFIVG
                     NRALVPDELEFVTRRGTLDRNDLASLAATLPAIRAGTTIDGLNIDLDDNGDWYIISAA
                     EAETRFGDATGALLTSDMIEVLEVVENEGGIVPTGEKLRTFLFAAENVAEEGEPLDLV
                     PRERFSGVPEFYLLHVTSELDAND"
BASE COUNT          704 a         1521 c         1393 g          589 t
ORIGIN      
        1 atcgacggcg gcccggacga cgacgtgctg atcggcaccg aggggaacga ccgtatcgac
       61 ggcatgagcg gcagcgacac gatctacggc cgctcgggca acgacctgct gtggggcgac
      121 tccaccgtcg gcgaggaggc gttctcgacc aacaccatcg acgtgatctt cggcggccgc
      181 ggcaacgacg acctgatcgg cgggccgggg atcaacgacc tctacgcctg gtcgaaggac
      241 ccgctgcccg acggcgacac cgagttcggc gtgttcgtcc acccgtcggc tccggacggc
      301 ccgctgttcg acgacagcca gggcggcacg ctcgtcctcg aggacaccgg catcgaccgc
      361 atgctcggca gccgcaacgc cgatcgcctg ttcggcggca ccaaggtgtc gttcctgttc
      421 ggcaacggcg gcgacgacca gctcttcacg gtcacgggcg agctgttcga gaccgccgac
      481 ggcggcctcg acgtcgacac gtggaagcag ttcgcgcagg cctcgggaca ggcgatctac
      541 gtcgcgggga ccgagaccga cgacgtcatc accgtggact tcgtcaccga gcccggcccg
      601 ctcgccggcc ggcacctgat cacccggctc accaacaaca acggcgtgtt caccttcgcc
      661 gcgcaggacg agttccagtt cctgctcgac gcggcgtccg acccggacaa cgtcgacacc
      721 gtcacgccgc tggagggcct gcagcagcgc ggcgggcagc aggaggatcc gctcaatccg
      781 gacctcacca tcgagacgcc gggcaccgag ccgcagtcgg ccggcaccga gcagggcggc
      841 accctcgagg ggctggtgcc ccagggcgga ggcgtcgagg tgatcatcat cgacgcgctc
      901 ggcggcgacg acctgatcac ggtcgggccg acggtgcagc actcggtgtg gatcgacgcc
      961 ggcgacggcg acgacgtggt gaagatccag ggcggcgagg tcatcctttc ggacaagacc
     1021 gagctcgtcc agccgagcaa ccccgttccc gtggaaggcg acgaggcgcg caacgactcg
     1081 gcggcgagcg cgttctacct cagccaggat ccgttcgccg cgcatccggc gaacgaagtg
     1141 gcgctgcccg gctcgaccac tttcagcggc ctgaacatcg acaacccgga cgacgaggac
     1201 tggtacaagt tctcgatcgc cgacgacgac ggcgacggtc tcgtgggcga gggcgcgctc
     1261 ctcaccctgg gcagcgtgtc ggacaacgac ggcctcgaga tcgcgctgtt cacgaccgac
     1321 ggcaccacga tgctcgagtc cggcctgctg cagctcagcc gcgacttcac cgacgtcggc
     1381 ggctcgccgg cgaacacgac cgcgagcgct ttcgagctgc cggcggtgca gtcgctcggg
     1441 cgcgcgagcg gcctgacgat ccacgacccg caggacgcgg atgtgttccg cttcgagctc
     1501 gagcgcgccg gcgtcagcgg cgaccgcatc aacctcctca agaactcgat cgacgacgac
     1561 ctggtgatcg agctgctcga cgcgggcggc gacgtcgtgg tggaaggcga ggaattcgcg
     1621 ccgctggtcg acgcggtgag cctcgagggc cttgccgcgg gcacctacta catccgggtc
     1681 tcgaccgaag gcgcgctcgc gcgctacgac ttgatcttcc aggtgcccgg cctgcgccag
     1741 accgagcgct cggtcacgcc tgcgccgaac gacagcgacg agaccccgtt cgacctcggc
     1801 gcgcactcca acttcccgac gctcaccggc acctcggtcg gcctcggcga ggaggagtgg
     1861 tacgcgttcg agatgctgcg cgacggctcg ccggccgacg aaatccgcat cgagtcctcc
     1921 gccttcgtgc agctgcggct cttccagcgc gaccaggacg gcgagcttga cgatctcgac
     1981 ctcgaccccg tagctgccga agccggaacc ccgggcgtca tctcgctcgc caccctcgac
     2041 aagggcgatt acctgctgcg cgtgtccggc gcccttggcc cgacgagcta cgagctcttc
     2101 ccgggcgacc gcactaccga gcgcaccatc gtcgagggcg tcgacggcac cggcgagctc
     2161 acgcgcaccg agatccgcac cctgcgagag ttcggcacga gtaatctcga cctcgccggc
     2221 gcggcggaga ccttcgtcga tctgtcgggc ctgaacgcga accagacgta tctgctgcgc
     2281 gtcacctcgc cgaaccgcat cccgacgatc tacgacctga ccttcgactt cagcagcgcc
     2341 gggtctccgg cggacctgag cgtcgagaag gagctggtga tcggcagctc gacgccgagc
     2401 gtaggcgagc ccgtcaagat cgaagtcaag gtcaccaacg acggcgacaa cgaagcgatc
     2461 aacgtcagcc tgaccgacct cctgcccgcc tggctgacgc cgacggcgag ccaggggatc
     2521 tacgatccgg cgacgggctt gtggcaggtc ggcttgctgt tcaccggagc gagcgcgacg
     2581 ctgaccttcg aaggtgtcgt tcccatgggg cagggcggca cgccgctgcc gctcgaagag
     2641 cccacggcta ccggcacggt cgcgctcgcc accgaccagc ccttcgaggt ggacctgagc
     2701 gtcaaggcga acaccgaccg gcgcgacgtc atcatcggcg gccccggcaa cgacgcgctg
     2761 cagggcggcc cgggctccac ctgggtgttc ggcatggcgg gcaacgacgt cctcaccgga
     2821 ggctacgacc ggcagtcgcc ggatctgctc ttcggcggcg agggcgacga cgcgttccag
     2881 ctcctgcccg accagctgcc gttcatcaag ggcacgaccg agacctacct gccgacgctg
     2941 gtcgaccgct tcgacggcgg cccgggcaac gacttcgtgc tcttccaggg cggcgacctc
     3001 gacaacctca accgcccggt ggacgactgg gtggcgatcc gctgggatcg cttccagcag
     3061 cgctacgagt tcaccgcggt gccgttcgac accgcgaacc aggtgttcgc ggtcgagcag
     3121 gaggtggtca acgccaccaa gctcgcgccg ctcgacggct tcgacggcga ggtcaacttc
     3181 cggctgcgcg tgccgattcc gggcatgccc gaccagggct tcgtcgagat gtcggtggac
     3241 atccaggacg ccgggaacat cagcgaactc gccgaccagc tgcaggcggc gctcgtcgcc
     3301 gagttcggcg tcgacgagaa cggcaacccg gtcgtcatcg tcgagttccc cgacggcatc
     3361 ctgcgcctgc gcgccagggt ccagggcctg gagctgcgcg ccgaggccga cgacctgatg
     3421 gtcaccgcgc tcggcttcga gccgctcacc gcgggctcgc cgatcttcct gcagcattac
     3481 gccttcttcc aggcgctgag cgtcgagaag atcgtgatcg acacgcgcgg cggcgacgac
     3541 atcgtgcacg ccgacccgga gtacttcttc ccgaacgtgc cgaccaccga gtggggcttg
     3601 gccgagggct cgttcgagga aggcgccaag atcccgctcg agatccgcct gggcgacggc
     3661 aacgaccgcg gcttcggcag ctcgcaggac gacacgatct tcggcggcga gggcgccgac
     3721 atcatcttcg gctacctcgg caacgacctc ctctacggcg gctcgggccg cgacttcatc
     3781 gtcggcaacc gcgcgctcgt gcccgacgag ctcgagttcg tcacgcgccg cggcaccctc
     3841 gaccgcaacg acctcgcgag cctcgccgcc acgctgcccg cgatccgcgc cggcaccacc
     3901 atcgacggcc tcaacatcga cctcgacgac aacggcgact ggtacatcat ctccgccgcc
     3961 gaggccgaga cccgcttcgg cgacgcgacc ggcgcgctcc tcacgagcga catgatcgag
     4021 gttctcgagg tggtcgagaa cgagggcggc atcgtgccca ccggcgagaa gctgcgcacg
     4081 ttcctgttcg ccgccgagaa cgtcgccgag gaaggcgagc cgctcgacct cgtgccgcgc
     4141 gagcgcttct ccggcgtgcc cgagttctac ctgctgcacg tcacgagcga gctcgacgcg
     4201 aacgacg
//
LOCUS       DASPFR010000334         4392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_101049, whole genome shotgun sequence.
ACCESSION   DASPFR010000334 DASPFR010000000
VERSION     DASPFR010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101049"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VD737_08800"
     CDS             complement(<1..820)
                     /locus_tag="VD737_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HXW10700.1"
                     /translation="MGRRADKRVDLKMPPGALHTYRRLLGYLKPHKGMFGIGVLGMTM
                     FAATDAGWAAFVKFFLDGTFVEKDERMVWLVPVALVGLFIVRGIGDFLQTYCPGYVGR
                     RIVKTLRGQIFDRYVHLPVAYFDRNASGVLLSKLTYNTEQVANATTESITVFIRDTLT
                     ILGLIGYLLYMNPRLTLFSLIVGPLIAFLIRRINLLFRRYSRRIQASMGDVTRVAKEA
                     IEAPRVIRVFNAQAYEAKLFEDVIEHNRRSHMKLMMTKGLSNPIVQTIAATGLAG"
     gene            complement(801..1229)
                     /locus_tag="VD737_08805"
     CDS             complement(801..1229)
                     /locus_tag="VD737_08805"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXW10701.1"
                     /translation="MKFQQRRHVEDEINMTSLIDVVLLLLIFFMMSTRFIDEARMQVR
                     LPEAGIQPDAESLRKTVEIEVTAEGGYRVEGRDLVNNSPDTLATALSRATGGNRAQVI
                     TIRADGRAVHQSVVTAMDVAGRLGYARINIATVHDGSQGR"
     gene            complement(1226..1903)
                     /locus_tag="VD737_08810"
     CDS             complement(1226..1903)
                     /locus_tag="VD737_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXW10702.1"
                     /translation="MFEIVKAGGPVMLPLILCSILAVAITLERLWTLRGRRVVPEELT
                     DKVWRWVENRSLSDKQIIALQQHSPLGKVLAAGLANRHRDRVLMVEAIEDAGRHVAHE
                     LERYLVALSTIAAISPLLGLLGTVTGMIRTFKAITVAGVGNPTAMASGIAEALITTAA
                     GLIIAIPALVAYRFLRARVDGHVIEMEKESLKLVAAIDRASSTRPGAASELAILQGET
                     GPQEAVA"
     gene            complement(1982..4252)
                     /locus_tag="VD737_08815"
     CDS             complement(1982..4252)
                     /locus_tag="VD737_08815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HXW10703.1"
                     /translation="MLCLPRWPGWSVLLACCAIGVLVAAWRRSWWPVAVAVSIAGTSW
                     TLSAALANRLDPALEGQSLSLRGTVVSVPQGTLESLRFRFAPDDGDAGSSTAPKLPRI
                     VELTWYDAPGRVLPAERLDLEVKLRRPRGFANPGGFDNEARMLRERIGASGYVRFGTR
                     LGRAHDAAWRHPVLLARGRVDAIVRDVLGTRPAAGIVAGLSVGLQDALSREQWLTLAR
                     SGTTHLMAISGLHIAMVGAVAAWLGARVQRWRQRLGALAAQRDAAVVAGALAALAYSL
                     LAGWSVPTQRTMVMIALGSVALALRRRVGIADGLGACVLAVLVADPLAPLSTGFWLSF
                     GAVATILFAATGFAHPPSPTASYLRVQAVVTVGLVPMLVGSFGAVSLVAALVNLYAVP
                     LYTLIVVPAVLLSCAVALASIDLGAPLLHATASLIEVTWPLLAVPAGWPLATWSIAGL
                     DAVAWCALLAGAAAALAPLPAAGRLAGIVLVTAACLSRPPPLSPGEASVTVLDVGQGL
                     SVVVETRGHVLLYDTGPSFRSGSDTGQLVVLPYLRHRGVRALDVLAVSHDDDDHAGGT
                     ASVLAMLPVRRLVRGPSIAADRLVVSGATRPARCRRGDRWQWDGVQFEWLHPGPEAYA
                     RDNDGSCVLRVRAGDRTALVTGDVEAQAEADLLATTALAPVDVLVAPHHGSRTSSTAP
                     FVAACRPAWVVYAVGHRNRWGFPYAGVVERYDAVGARGLRTSSSGALTFRLRPGRPVE
                     PPQQWRRESLRTWRDS"
BASE COUNT          720 a         1565 c         1527 g          580 t
ORIGIN      
        1 cgcccgcgag gcccgtcgcc gcgatcgtct gcacgatcgg gttgctcaac cccttggtca
       61 tcatcagctt catgtgcgag cggcggttgt gctcgatcac gtcctcgaac agcttcgcct
      121 cgtacgcctg ggcgttgaag acgcgaatca cccgtggcgc ctcgatcgcc tccttcgcga
      181 cccgtgtcac gtcgcccatc gacgcctgga tccggcggct gtagcgtcga aacagcaggt
      241 tgatcctgcg gatcaggaac gcgatcagcg gacctacgat caggctgaac agcgtgagcc
      301 tgggattcat gtacagcagg tagccgatca gcccgaggat cgtcagcgtg tcgcggatga
      361 agacggtgat cgactcggtc gtcgcgttgg cgacctgctc ggtgttgtac gtgagtttcg
      421 agagcagcac gccggacgcg ttgcggtcga agtacgcgac cggcaggtgc acgtagcggt
      481 cgaagatctg cccgcgcagc gtcttgacga tgcggcgccc gacgtagccg ggacagtacg
      541 tctgcaggaa gtcgccgatg ccgcgcacga tgaacagccc gaccagcgcg accggcacga
      601 gccacaccat gcgctcgtcc ttctcgacga acgtgccgtc gaggaagaac ttgacgaacg
      661 cggcccagcc cgcgtcggtc gcggcgaaca tggtcatgcc gagcacgccg atcccgaaca
      721 tgcccttgtg cggcttcagg taaccgagca gccgcctgta ggtatgcagc gcccccggcg
      781 gcatcttcag gtcgacgcgc ttatcggccc tgcgacccat cgtggaccgt ggcgatgttg
      841 atgcgggcgt agccgagccg tcccgcgacg tccatcgccg tgacgacgga ctggtgcacg
      901 gcccggccgt cggcccggat cgtgatcacc tgggcccggt tgccgccggt cgcgcgcgac
      961 agcgcggtcg cgagcgtgtc gggactgttg ttgacgaggt cgcggccttc gacgcgatag
     1021 ccgccttccg cggtgacctc gatctcgacg gtcttgcgca aggattcggc gtccggctgg
     1081 atgccggcct ccggcaggcg cacctgcatg cgggcctcgt cgatgaaccg cgtcgacatc
     1141 atgaagaaga tcagcagcag cagcacgacg tcgatcagcg acgtcatgtt gatctcgtcc
     1201 tcgacgtgcc ggcgctgctg gaacttcaag cgacggcttc ctgcggcccg gtctcgccct
     1261 gcaggatggc gagttcggac gcggcgccgg gacgcgtcga gctcgcgcgg tcgatcgccg
     1321 cgacgagctt gagcgattcc ttctccatct cgatcacgtg cccgtccacg cgcgcgcgca
     1381 ggaagcggta ggcgaccagc gcggggatcg cgatgatcag gccggcggcc gtagtgatca
     1441 gcgcctcggc gatgccgctc gccatcgcgg tcgggttgcc gacgccggcg acggtgatcg
     1501 ccttgaaggt gcggatcatg ccggtcaccg ttcccagcag cccgagcagc ggcgagatcg
     1561 cggcgatcgt cgacagcgcg acgaggtagc gctcgagctc gtgggcgacg tgccggccgg
     1621 cgtcctcgat cgcctcgacc atcagcacgc gatcgcggtg gcgattggcg agtcccgcgg
     1681 cgagcacctt gccgagcggc gaatgctgct gcagcgcgat gatctgcttg tcggagagcg
     1741 agcggttctc gacccagcgc cacaccttgt cggtgagctc ttcggggacg acgcgccggc
     1801 cgcgcaacgt ccacaggcgc tccagcgtga tggcgaccgc caggatggag cagaggatga
     1861 gcggcagcat gaccgggccg ccggccttga cgatctcgaa catgtagtcg ctggagtcct
     1921 gccccgggaa agccgcacgg gaatcatact tgatgcccct cgccccggcg acgggcccgt
     1981 ttcaggaatc gcgccaggta cgtagcgatt ccctgcgcca ctgctgcggt ggctcgaccg
     2041 gccggcccgg tcgcagtcga aacgtgagcg cgccgctcga gctcgtgcgc aggccgcgtg
     2101 cacccacggc gtcgtaacgc tcgaccacgc ccgcgtacgg gaatccccag cggttgcggt
     2161 gacccacggc gtagacgacc caggccggcc ggcatgcggc gacgaacggc gccgtcgaag
     2221 acgtccggct gccgtgatgc ggcgccacca gcacgtccac gggcgcgagt gccgtggtgg
     2281 cgagcaggtc cgcttctgcc tgcgcctcca cgtcgccggt gaccagggcg gtgcggtctc
     2341 cggcgcgcac gcgcaggacg caggagccgt cgttgtcccg ggcgtacgcc tccggccccg
     2401 ggtgaagcca ctcgaattgc actccgtccc actgccatcg atccccgcgt cggcagcgcg
     2461 ccgggcgcgt cgcgccggag acgacgagac gatcggcggc gatgctcggc ccgcgcacga
     2521 ggcggcgcac cggcagcatc gccagcacgc tcgccgtgcc gccggcgtga tcgtcgtcgt
     2581 cgtggctcac ggccagcacg tccagcgcac gcacgccgcg gtgccgcagg tacggcagca
     2641 ccacgagctg ccccgtgtcg ctgccggagc ggaacgaggg gcccgtgtcg tacagcagca
     2701 cgtgcccgcg cgtctcgacc acgaccgaca gcccctgccc gacgtcgagc accgtgaccg
     2761 acgcctcgcc gggcgacaac ggtggcggcc gggacaggca cgccgcggtc acgagcacga
     2821 ttcccgccag gcggcccgca gccggcaacg gcgcgagcgc cgccgcggct cctgcgagca
     2881 gggcacacca ggccaccgcg tcgaggcccg cgatcgacca ggtcgcgagc ggccagcctg
     2941 ccggcaccgc gagcagcggc cacgtgacct cgatcaacga cgccgtcgcg tgcagcagcg
     3001 gcgcgccgag gtcgatcgac gcgagcgcca cggcgcacga gagcagcacg gccggcacga
     3061 cgatgagcgt gtagagcggc accgcgtaca ggttcacgag cgcggcgacc agtgacaccg
     3121 cgccgaaact gccgacgagc atcggcacca gccccaccgt gaccacggcc tgcacccgca
     3181 ggtaactcgc agtcggcgac ggcggatgcg cgaaacccgt cgccgcgaac aggatcgtcg
     3241 cgacggcacc gaacgacagc cagaagcccg tcgacagcgg cgccaacgga tcggcgacca
     3301 gcacggcgag cacgcaggcg cccaggccgt ccgcgatgcc gacgcgtcgt cgcaacgcga
     3361 gcgcgaccga tccgagcgcg atcatgacca tcgtccgctg cgtcggcacc gaccagcctg
     3421 cgagcaggct gtatgccaat gcggcgagcg cacccgcgac cacggccgcg tcacgttgcg
     3481 ccgccagcgc gccgagtcgc tggcgccagc gctgcactcg ggcgccgagc caggctgcga
     3541 cggctccgac catcgcgatg tgcaggcccg aaatcgccat caggtgggtc gtgccgctgc
     3601 gcgcgagcgt gagccactgc tcgcggctga gcgcatcctg caaccccacc gacaggccgg
     3661 cgacgatgcc agctgccggg cgcgtcccca gcacgtcgcg cacgatcgcg tcgacacgac
     3721 cgcgcgcgag cagcaccggg tgtcgccagg ccgcgtcgtg cgcgcggccg agccgcgtgc
     3781 cgaagcgcac gtagccgctg gccccgatgc gctcgcgcag catgcgggcc tcgttgtcga
     3841 aaccaccggg gttggcgaag ccgcgcgggc gtcgcagctt gacctcgaga tcgaggcgtt
     3901 cggccggcag cacgcgaccg ggcgcgtcgt accaggtcag ctcgacgatc ctggggagtt
     3961 tcggtgcggt gctcgaacct gcgtcaccat cgtcgggcgc gaagcggaac cgcaacgatt
     4021 cgagcgtgcc ctgcggcacg ctgaccaccg tgccacgcaa actgagcgac tgcccttcga
     4081 gcgccggatc gaggcggttg gcgagcgccg cgctcagcgt ccacgaagtc ccggcgatgg
     4141 acacggccac ggcgacgggc caccacgacc gccgccacgc tgccacgagc acgccgatcg
     4201 cgcagcacgc gagcagcacc gaccaccctg gccagcgcgg caggcagagc acgagacagc
     4261 agcccgccaa cgctccgaga gcagcacgcc acatcgtgtc gagcgtggcg cggcgcggag
     4321 gaagccgcga gcgactaaag cgggcggaag gcgtcgaaag tgactggtga ctaggtgaat
     4381 agtgactagt cg
//
LOCUS       DASPFR010000335         3646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1076810, whole genome shotgun sequence.
ACCESSION   DASPFR010000335 DASPFR010000000
VERSION     DASPFR010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076810"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VD737_08820"
     CDS             <1..351
                     /locus_tag="VD737_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10704.1"
                     /translation="RLESLLALYERRFGEVLTAVDLAIERELAGDARAVTELYERLPD
                     IDFSRHVLAGQEDFLRVVHVPACGWSDLGTPHRVAAALRSPRPAPHVIAPHITPAAIL
                     DLAAQHGRLQLANA"
     gene            377..1267
                     /locus_tag="VD737_08825"
     CDS             377..1267
                     /locus_tag="VD737_08825"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HXW10705.1"
                     /translation="MIRPVSFRLLATAATAFLATNSVAAAGSVTSAPTLTLEGARQIL
                     ADATAYARAHGAPGGAIAVVDAAGILVSLDRLDGTFPSASNISIGKARTAALFRKPTR
                     DFETLVNQGRYTMTTLPDLTPFTPLQGGVPITANGAVIGAVGVSGAASAKQDDEIAQA
                     AVDAFAKAQAATAAIHHVPRATVEQAYRDDANLVTADGFRVNASRRDRPGDAEVHLHE
                     TDIFYVREGRATLVTGGTVVEPRNVSATEIRGRAIDQGEERRLEAGDVITVPSGVPHW
                     FKSVEAPFTYYVVKSSAVGG"
     gene            1270..2892
                     /locus_tag="VD737_08830"
     CDS             1270..2892
                     /locus_tag="VD737_08830"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXW10706.1"
                     /translation="MKRAAWLLALLTASTAAAAADPSAAPTGVPAARLDLQTTEGVAA
                     VRGVWRYADAELVPTNHRAPDPNGQPTGRPVKTWDVQPHAGGATFDDSRWAAIEPATL
                     RERRGNGRVSFNWYRLEVTVPERVGTFDTRGATLVFSTSLDDYAEVWVDGELPRATGQ
                     NGGSVVAGWNATNRIVIGRNVHPGQQIQLAVFGINGPISDPPANFIWMRHAALEFFPG
                     GPVPLAVPPQEVNITVERLDPAIDTLVPANAKLFKLAEGFLFTEGPVWVRDGGYLLFS
                     DPNANRIYAYAEESATLSLYLPQSGYDGADVAEYSQPGSNGLALDAQGRLTINQHGHR
                     RVVRVEPDGQLTVLAAADREGRRLNSPNDLVYRADGSVYFTDPPFGLPKFFDDPRKEI
                     PHSGVYRAKDGVVTLLAQDLQGPNGLAFSGDERWLYVDNWDPEAKVVLRYPVRDDGTL
                     GVPETFADLTREVPGDEALDGLKVDAEGNVWVAGPDGLRVYSAAGRHLGTIRAPRPIH
                     NFAWGGADGRTLYVTARDRLYRMPVLVGGARP"
     gene            2889..>3646
                     /locus_tag="VD737_08835"
     CDS             2889..>3646
                     /locus_tag="VD737_08835"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXW10707.1"
                     /translation="MTRRCPCHVVHRPRVPGRGAILAWLAIVATWLIGLGNDAWGADG
                     AVAPFPSERGRIVRLDARLDALLPVDATLETVASGHRWLEGPLWDARRNALLYSDIPA
                     NAVYRWQAGSRPAVFLARSGYTGAAPFTGREPGSNGLAFDREGRLVLCQHGDRRIVRL
                     ERDGTRTVLVDAYEGRRLNSPNDAIYASNGDLYFTDPPFGLPRAFDDPARELDVSGVY
                     RLRADGTLELLIGDLGAPNGIALSPDERTLYVTDV"
BASE COUNT          528 a         1321 c         1264 g          533 t
ORIGIN      
        1 cggctcgagt cactgctggc cctgtacgaa cgccgcttcg gcgaggtgct gaccgcggtc
       61 gacctcgcga tcgaacgtga gctcgccggc gacgcccgcg ccgtgaccga gctgtacgag
      121 cgactgccgg acatcgactt ctcgcgccac gtgctggcgg gccaggagga cttcctgcgc
      181 gtggtccacg tgccggcctg cggctggagt gatctcggca cgccgcaccg cgtcgccgcc
      241 gccttgcgca gcccgcggcc cgcaccgcac gtgattgcac ctcacatcac gccggccgcg
      301 atcctcgacc tcgcggccca gcacggacgg ctgcagctcg ccaacgcctg acctggcctg
      361 cacgaaggag aaacacatga tccgccccgt ttcgtttcga ctgctggcca ccgctgcgac
      421 ggcattcctc gcgacgaact cggtcgcggc agccggttcg gtgacgagtg ccccgacgct
      481 cacgctcgaa ggcgcccgcc agatcctcgc ggacgcgacg gcttacgcac gtgcgcatgg
      541 tgcgcccggc ggcgccatcg ccgtggtcga tgcggccggc atcctggtga gcctggaccg
      601 gctcgatggc acgtttccgt cggcctcgaa catctcgatc ggcaaggcgc gcaccgctgc
      661 cctcttccgc aagccgacgc gggatttcga gacgctcgtc aaccagggcc gctacacgat
      721 gacgacgctg cccgacctga cgccgttcac gccgctgcag ggcggcgtgc cgatcacggc
      781 gaacggcgcc gtgatcggtg ccgtcggggt gagcggtgct gcgagcgcga aacaggacga
      841 cgagattgcg caggccgcgg tcgatgcgtt cgccaaggct caggcggcga ccgccgcgat
      901 ccaccacgtg ccgcgcgcaa ccgtcgagca ggcctatcgc gacgacgcga acctcgtgac
      961 cgcggacggc tttcgcgtca acgcgagccg gcgcgaccgc ccgggcgacg cggaagtgca
     1021 cctgcacgag accgacatct tctacgtgcg cgagggccgc gcgacgctcg tgaccggcgg
     1081 caccgtcgtc gagccgcgca acgtgtctgc gacggagatc cgcggccgcg cgatcgacca
     1141 gggcgaggaa cgccggctcg aggccggtga cgtgatcacc gtgccgagcg gcgtgccgca
     1201 ctggttcaag tcggtcgagg cgccgttcac ctactacgtc gtcaagtcct cggcggtggg
     1261 aggttgatcg tgaagcgcgc tgcgtggttg cttgccctgc tcaccgcctc gaccgcggcg
     1321 gcggcggccg atccctcggc cgcgccgacg ggcgtaccgg ccgcgcggct cgacctgcag
     1381 acgaccgagg gcgtcgccgc ggtgcgcggc gtatggcgct acgccgacgc cgagctcgtg
     1441 cccacgaatc atcgtgcgcc tgacccgaac ggccagccga ccggccggcc ggtgaagacc
     1501 tgggacgtgc agccgcatgc cggcggcgcg acgttcgacg attcgcgctg ggcggcgatc
     1561 gagcccgcca ccctgcgcga gcgtcgcggc aacggccgcg tgtcgttcaa ctggtaccga
     1621 ctcgaggtga cggtgccgga gcgcgtgggc acgttcgaca cgcgcggcgc gacgctcgtc
     1681 ttctcgacct cgctggacga ctacgccgag gtgtgggtcg acggcgaact gccgcgggcc
     1741 accggtcaga acggcggctc ggtggtcgcc ggctggaacg cgaccaaccg gatcgtgatc
     1801 ggacgcaacg tgcacccggg ccagcagatc cagctcgcgg tgttcggcat caacgggccg
     1861 atctccgacc cgccggcaaa tttcatctgg atgcgccacg cggcgctcga gttcttcccg
     1921 ggtgggcccg tgccgctggc cgttccgccg caggaggtca acatcacggt cgagcgcctc
     1981 gacccggcga tcgacacgct cgttcccgcg aacgccaagc tgttcaagct ggccgaaggg
     2041 ttcctgttca ccgaaggtcc cgtgtgggtg cgcgacggcg ggtacctgct cttcagcgat
     2101 ccgaacgcca accggatcta cgcgtacgcg gaagagtccg cgacgctgtc gctgtatcta
     2161 ccgcagagcg gctacgacgg agcggacgtc gccgagtact cgcagccggg ctcgaacggc
     2221 ctggcgctcg atgcgcaggg ccggctcacg atcaaccagc acgggcaccg ccgggtggtg
     2281 cgcgtcgagc ccgacggcca gctgacggtg ctcgccgccg ccgaccgcga gggtcgccgg
     2341 ctcaacagcc cgaacgatct cgtctatcgc gccgacggca gcgtctactt caccgatccg
     2401 ccgttcgggc tgccgaagtt cttcgacgat cctcgtaagg aaatcccgca ctccggggtc
     2461 tatcgggcca aggacggtgt cgtgacgctg cttgcgcagg acctgcaggg gccgaacggt
     2521 ctcgccttct cgggcgacga gcgctggctc tacgtcgaca actgggatcc ggaggccaag
     2581 gtcgtgctgc gatatccggt ccgggacgac ggcacgctcg gtgtcccgga gacgttcgcg
     2641 gacctgacgc gcgaggtgcc gggcgacgaa gcgctcgacg gcctgaaggt cgacgccgaa
     2701 ggcaacgtct gggtcgcggg gcccgacggc ctgcgcgtct actcggcggc cggacggcac
     2761 ctcggcacga tccgcgcgcc gcgcccgatc cacaacttcg cctggggtgg tgcggacggc
     2821 cgtacgctct acgtgacggc gcgcgaccgt ctctatcgca tgccggtgct cgtcggcgga
     2881 gcgcggccat gacgcggcgc tgcccgtgcc acgtcgtgca ccgtccgcgc gtgccgggcc
     2941 gcggcgcgat cctggcgtgg ctcgcgatcg tcgcgacctg gctgatcggc ctcggcaacg
     3001 acgcctgggg cgccgacggc gccgtcgcac cgttcccgtc cgagcgcggc cgcatcgttc
     3061 gactcgatgc gcggctcgac gcgctgctgc cggtcgacgc gaccctcgag acggtggcga
     3121 gcggtcatcg ctggctcgaa ggtccgctct gggacgcgcg caggaatgcc ctcctctatt
     3181 cggacattcc ggccaacgcc gtctatcgat ggcaggccgg cagccgcccc gccgtgttcc
     3241 tcgcacgcag cggttacacc ggagcggcac cgttcacggg acgtgagccc ggttcgaacg
     3301 gactggcctt cgaccgggaa ggccggctcg tgctgtgcca gcacggcgac cgtcgcatcg
     3361 tgcggctcga gcgcgacggc acgcgcaccg tgctcgtcga cgcctacgaa ggtcgccggc
     3421 tcaacagccc gaacgacgcg atctatgcct cgaacggcga tctctacttc accgacccgc
     3481 cgttcggttt gccccgtgcg ttcgacgatc ccgcgcgcga gctcgacgtc agcggcgtct
     3541 atcggctgcg cgccgacggc acgctcgaac tgctgatcgg cgacctcggc gcgccgaacg
     3601 gcatcgcgct gtcgccggac gagcgcacgc tctacgtgac cgacgt
//
LOCUS       DASPFR010000336         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1809487, whole genome shotgun sequence.
ACCESSION   DASPFR010000336 DASPFR010000000
VERSION     DASPFR010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1809487"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1932
                     /gene="xoxF5"
                     /locus_tag="VD737_08840"
     CDS             112..1932
                     /gene="xoxF5"
                     /locus_tag="VD737_08840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthanide-dependent methanol dehydrogenase
                     XoxF5"
                     /protein_id="HXW10708.1"
                     /translation="MTKSRLLSSVAALLVTGVPAVANDSVMEAISDPGNWAIPTGDYA
                     NTRYSELDQINRDNVGDLQVAWTFSTGVLRGHEGSPLVIGNMMYVHTPFPNIVYALDL
                     DKEAQIVWKYEPKQDPSVIPVMCCDTVNRGVAYADGTIILNQADTTVVALDAKTGKEQ
                     WKAVNGDPKKGETHTATTLIVKDKVIVGISGAEFGVRCHMTAYDLKSGKRVWRAYSMG
                     PDEEILVDPEKTTSLGKPVGKDSSLKTWNGDQWKIGGGSTWGWYSYDPEVNLFYYGTG
                     NPSTWNPAQRAGPDGKPIDQKWSMTIFARDPDTGMAKWAYQMTPFDEWDYDGINEMIL
                     ADIDVGGQKRKTLVHFDRNGLGYTLDRVTGELLVAEKYDPVVNWTTGVVMDKASPQYG
                     RPQVVAKYSTFQQGQDVNTKGICPAALGTKDQQPAAFSPKTGHFYVPTNHVCMDYEPF
                     KVSYTAGQPYVGATLSMYPAPGSHGGMGNFIAWDAGKGKIVWSKPEQFSVWSGALSTA
                     GGVVFYGTLEGYLKAVDEKDGKELFKFKTPSGIIGNVMTYSHKGKQHVGILSGIGGWA
                     GIGLAAGLTNPNDGLGAVGGYAGLKNYTNLGGVLTVFTLP"
BASE COUNT          406 a          703 c          723 g          334 t
ORIGIN      
        1 aacttccttg tcgagcgatg ctccggctcc ccggggataa gcgggtccgt cttgcgagcc
       61 acatgacggg acaaggccgg ttctagatgg ctcgcttctg aggggggaga aatgaccaag
      121 agtcgtctct tgtcgtccgt cgccgcgctc ttggtgacgg gcgtgccggc ggtcgccaac
      181 gacagcgtga tggaagcgat cagcgatccc ggcaactggg cgatcccgac cggggactac
      241 gccaacacgc ggtactcgga gctggaccag atcaaccgcg acaacgtcgg tgacctgcag
      301 gtggcctgga cgttctcgac cggcgtgctg cgcggccacg agggctcgcc gctggtcatc
      361 ggcaacatga tgtacgtgca cacgccgttc ccgaacatcg tgtacgcgct cgacctcgac
      421 aaggaggcgc agatcgtctg gaagtacgag ccgaagcagg atccgtccgt gattccggtg
      481 atgtgctgcg acacggtcaa ccgcggcgtc gcctatgccg acggcacgat catcctgaac
      541 caggccgaca ccaccgtcgt ggcgctcgac gccaagaccg gcaaggagca gtggaaggcc
      601 gtcaacggcg acccgaagaa gggcgagacg cacaccgcga cgacgctgat cgtcaaggac
      661 aaggtgatcg tcggcatctc cggcgccgag ttcggtgtgc gctgccacat gaccgcctac
      721 gacctcaaga gcggcaagcg cgtctggcgc gcctactcca tgggtccgga cgaggagatc
      781 ctcgtcgatc cggagaagac gaccagtctc ggcaagccgg tcggcaagga cagctcgctc
      841 aagacttgga acggcgacca gtggaagatc ggcggcggct cgacgtgggg ctggtactcc
      901 tatgatccgg aggtcaacct gttctactac gggacgggca atccttcgac ctggaacccc
      961 gcgcagcgcg caggacccga cggcaagccg atcgaccaga agtggtcgat gacgatcttc
     1021 gctcgtgacc cggatacggg catggcgaag tgggcttacc agatgacgcc cttcgacgag
     1081 tgggactacg acggcatcaa cgagatgatc ctcgcggaca tcgacgttgg cggccagaag
     1141 cgcaagacgc tcgtccactt cgaccgcaac ggcctcggct acacgctcga tcgcgtgacc
     1201 ggcgagctgc tcgtcgccga gaagtacgat ccggtcgtca actggacgac gggcgtcgtc
     1261 atggacaagg cgagcccgca gtacggccgt ccgcaggtcg tggcgaagta ctcgaccttc
     1321 cagcagggcc aggacgtgaa caccaagggc atctgcccgg cggctctggg cacgaaggac
     1381 cagcaaccgg cggcgttctc gccgaagacc ggtcacttct acgtgccgac caaccacgtc
     1441 tgtatggact acgagccctt caaggtctcg tacacggccg gtcagcccta cgtcggcgcc
     1501 acgctcagca tgtatccggc tccgggcagc cacggcggca tgggcaactt catcgcctgg
     1561 gacgcgggca agggcaagat cgtgtggtcg aagcccgagc agttctcggt gtggtcgggt
     1621 gcgctcagca ccgcgggcgg cgtcgtcttc tacggcacgc tcgagggcta cctgaaggcg
     1681 gtcgacgaga aggacggcaa ggagctgttc aagttcaaga cgccgtccgg catcatcggc
     1741 aacgtcatga cgtactcgca taagggcaag cagcacgtcg ggatcctgtc gggcatcggc
     1801 ggctgggcgg gcatcggcct cgcggccggc ctcaccaacc cgaacgacgg cctcggcgcg
     1861 gtcggcggct acgcgggcct caagaactac accaacctcg gtggcgttct gacggtcttc
     1921 acgctgccct aacgcactcg gcattgggga cggacgcgag ccggcgccgg ggaaacctgg
     1981 cgccggtttc gtttgggcct ccaatttgcg acgacggtcg aggtgccccg caggcgccct
     2041 ctgcgagggg ccgccgcggg agccggccgc cctcgctgac gcgccctccc ctgctgctca
     2101 cgctgaccgc ccggcgagcg ctgtcgagga tcagcatgga gagttccgct cgtcacctgc
     2161 gtagcg
//
LOCUS       DASPFR010000337         3164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1689052, whole genome shotgun sequence.
ACCESSION   DASPFR010000337 DASPFR010000000
VERSION     DASPFR010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689052"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..685
                     /locus_tag="VD737_08845"
     CDS             239..685
                     /locus_tag="VD737_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10709.1"
                     /translation="MPHAVHWEPEGVCVRFWGTVDSSEITAIYEEISGDERSDRIRFV
                     LQDYLGATRSTGMTLSDVKVFVALEMGASYGQAHSVWRAAIATDPSICDFLKYFRSVR
                     TSPDPFQVFSTEREAREWFKTNPYLRNKAAPRCEDSERPSSKRSAH"
     gene            complement(819..1400)
                     /locus_tag="VD737_08850"
     CDS             complement(819..1400)
                     /locus_tag="VD737_08850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HXW10710.1"
                     /translation="MSDTYQVGYLVGSLARASINRRLARALIRLAPKPLEFHEIEFKD
                     LPLYTYDYDADYPPVARAYKAAIAKADALLFVTPEYNRSIPGGLKNAIDWASRPKGQN
                     SFSRKPSAVIGTSPGAIGTAVAQQQLRSVLSFCNSPQMNSPEAYIQFKDGLIDDQGRV
                     SDDSTTDFLRKYMEEFHGFIERVLTVLPRAARA"
     gene            complement(1440..2402)
                     /locus_tag="VD737_08855"
     CDS             complement(1440..2402)
                     /locus_tag="VD737_08855"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXW10711.1"
                     /translation="MTRKSDSPALRTSSSVLQQILDELEQATLEHALWRDHLVRVIAG
                     RETSNPQDLAPDAHRHCLFGRWYFERSPEDLRQRPSYAMIGAEHADQHRLAASLLQSV
                     ADGVPVSRATIEEFEEASARLSYALYFIKRELECALSSRDAITEAHSSGEMIRDLREW
                     HALARRPGRQCCIALMELDDQRELDALHGFSAGAQALVNAVRIVASHLRGSDRVFRYD
                     GRKFLIRLSGTELAPARTVIGRLREAVDGALASPGANGVVVRRTASFGIAQLDPDVDV
                     LESIDRADQALTLAKAAGGNRTITWDPSITTARLRRVEVKDVQG"
     gene            complement(2508..>3164)
                     /locus_tag="VD737_08860"
     CDS             complement(2508..>3164)
                     /locus_tag="VD737_08860"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXW10712.1"
                     /translation="WGHVAHVIAVRYRSRALFALTLMASQAFFYNAIFFTYALVLGRF
                     FGVPDHRVGLYILPFAAGNFLGPLLLGRWFDVVGRKPMIVFTYAASGVLLAIVAALFN
                     AGWLTATGQTVAWCVVFFFASAAASSAYLTVGEVFPLEMRALSIAIFYTVGTGVGGVA
                     GPWLFGALIGTGERGLIAVGYLLGSALMLVAAALAARLVVPAERRALEEVAPPLSRAP
                     "
BASE COUNT          531 a         1078 c         1081 g          474 t
ORIGIN      
        1 cgccgtcggg gcgcgacgag atgtatcgaa gcctgcgctg acgcgacgga gctcgtcgac
       61 accggcgagt cgccccggag agtcgccgcc cctggacggg cgcgcgcgtg aggcgcaacg
      121 ttttgtgagt caaggcttac ctgcgggcgc agtgcggacg cgacgcctgc ccgctcaccg
      181 acagccgcgc acccggcggc cgctagtccc ggctggcatt ttccggaaaa ctaggtcgat
      241 gccgcatgcc gtccattggg aacccgaggg cgtctgcgtc aggttctggg gcaccgtcga
      301 ctcgtcggaa atcacggcga tctacgaaga gatctccggc gatgagcgct cggaccgcat
      361 acgcttcgtg ctgcaggact acctcggcgc gacgcgctcc acgggcatga cgctgtccga
      421 cgtcaaggtg ttcgtcgcgc tcgagatggg cgcgtcgtat ggccaggcgc actcggtctg
      481 gcgcgccgcc atcgcgaccg atccatccat ctgcgatttc ctgaagtact ttcgcagcgt
      541 gcgcacgtct cccgatccgt tccaggtctt ctcgaccgaa cgcgaggcgc gcgagtggtt
      601 caagacgaat ccatacctca ggaacaaggc cgctccgcgc tgcgaggaca gtgagcgccc
      661 gagctcgaag cgaagcgcgc attgatccct gccggcgtac gactggtacg acgcactgaa
      721 ggtggtcgag ggcaacccgg cgtggtgatc cgcgccggcg cgctcctccc cgcagagccc
      781 gcctctccga cgggctcttc ctgcatggag gccgcggctc aggccctggc ggcccggggc
      841 agcaccgtga gcacgcgctc gatgaagccg tgaaactctt ccatgtactt gcgcaggaag
      901 tcggtcgtcg agtcgtcgct gacccggccc tggtcgtcga tcaggccgtc cttgaactgg
      961 atgtaggctt ccggcgagtt catctgcggc gaattgcaga acgagagcac gctccgcaac
     1021 tgctgctggg cgacggccgt gccgatcgcc cctggcgacg tgccgatcac ggccgaaggt
     1081 ttgcgagaga acgagttctg tcctttcgga cggctcgccc agtcgatcgc gttcttgagg
     1141 ccgcccggaa tcgagcggtt gtactcaggc gtcacgaaca gcagcgcatc cgccttggcg
     1201 atcgccgcct tgtaggcccg cgcgaccggc gggtagtcgg cgtcgtagtc atacgtgtag
     1261 agcggcaggt ccttgaactc gatctcgtga aactcgagcg gtttcggcgc aagccggatc
     1321 agcgcccggg cgaggcgccg gttgatcgag gcacgggcga ggctgccgac gagatagccc
     1381 acctggtacg tgtcgctcat cgtgcgcccc ctccttggcc cctgcccgtc catgcgggcc
     1441 tagccctgga cgtccttgac ctcgacgcgg cgcagccgcg cggtggtgat cgacgggtcc
     1501 caggtgatcg tgcgattgcc gccggcggcc ttggcgagcg tcagcgcctg gtccgcgcgg
     1561 tcgatggact ccagcacgtc cacgtccggg tcgagctgcg cgatgccgaa cgaggcggtt
     1621 cgacgcacca ccacgccatt cgcgccgggg gaagccaggg cgccgtccac cgcttcgcgc
     1681 agccgcccga tcaccgtccg ggcgggcgcg agttcggtgc ccgacaggcg gatcaggaac
     1741 ttgcggccgt cgtagcggaa cacgcgatcc gaaccacgca ggtgcgaggc gacgatcctc
     1801 acggcattga ccagcgcctg cgcgccagcc gagaagccgt gcagcgcgtc gagttcccgc
     1861 tggtcgtcga gctccatgag cgcgatgcag cactgccgcc ccggtcgacg tgcgagggca
     1921 tgccactcgc gcaggtcgcg gatcatctcg cctgagctgt gggcctccgt gattgcatcg
     1981 cgactgctga gcgcgcactc gagctcgcgc ttgatgaaat agagcgcgta gctcaaccgg
     2041 gcactcgctt cctcgaactc ctcgatggtc gcgcgggaca ccggcacgcc atcggccacg
     2101 ctctgcagca ggctggcggc caggcggtgc tggtccgcgt gctccgcgcc gatcatcgcg
     2161 tacgagggac gctggcgcag gtcctccggt gaccgctcga agtaccagcg gccgaacagg
     2221 caatgccggt gcgcgtccgg cgcgagatcc tgcggattgg atgtctcgcg acctgcgatc
     2281 acgcgcacca ggtggtcgcg ccacagcgcg tgctcgagcg tggcctgctc gagttcgtcg
     2341 agtatctgct gcaggaccga cgagctcgtc ctgagcgcgg ggctgtctga cttccgggtc
     2401 atcgggtcca gagcataccc gttctgccgt gcgccgacgt tcggtcgaag tcgcacgatt
     2461 ccgcaataat cagagcgcag cggactgtag gcaggtgggg agccggtcta cggcgcccgc
     2521 gaaagcggcg gcgccacctc ctcgagcgcg cgacgctcgg cgggcaccac gagcctggcc
     2581 gcgagggcgg ccgcgaccag catcagcgcc gatcccagca gatagccgac cgcaatcaag
     2641 ccgcgctcgc ccgtgccgat cagcgccccg aacaaccacg ggccggccac gccgccgacg
     2701 ccggtgccca ccgtgtagaa gatcgcgatc gacagcgcgc gcatctcgag cggaaacacc
     2761 tctccaacgg tcaggtaggc ggagctggcg gcggccgagg caaagaagaa cacgacgcac
     2821 cacgccacgg tctgcccggt ggcggtgagc cagcccgcgt tgaacagcgc ggcgacgatc
     2881 gcgagcagca cgcccgaggc cgcgtaggtg aaaacgatca tcggcttgcg cccgacgacg
     2941 tcgaaccagc gtcccaacag cagcggcccg aggaagttgc cggccgcgaa gggcaggatg
     3001 tacaggccga cgcgatggtc gggaacgccg aagaagcgac cgagcacgag cgcatacgtg
     3061 aagaagatcg cgttgtagaa gaacgcctgc gacgccatca gcgtgagcgc gaacagcgcg
     3121 cgcgagcggt agcgcacggc gatgacgtgc gccacgtgcc ccca
//
LOCUS       DASPFR010000338         5425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_833657, whole genome shotgun sequence.
ACCESSION   DASPFR010000338 DASPFR010000000
VERSION     DASPFR010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833657"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..1236)
                     /locus_tag="VD737_08865"
     CDS             complement(208..1236)
                     /locus_tag="VD737_08865"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HXW10713.1"
                     /translation="MRAERGDAAPPILLRRATTPPEVTLLLRAGLVLLLIASVFLVFL
                     IDRAGLQDNIDGHVSVSDVFYFTMVTVTTVGYGDIVPVSDQARLIDAFFVTPVRMFIW
                     LLFLGTAYQLVLQRFFEEWRMLRLQRELKGHVIICGYGHSGSIAANELLLRGWDGERV
                     VVIDRDREEVAKAAERGLIGIHGDASSEELMRMAGLREAQSLLVSVGRDDTTVLVVLT
                     ARSLTKQVRIIASVREPENIKLVRAGGADEVVSPPRFGGFLMADAVATHGTVDFVSEL
                     LSYRGDYQLVERDVRPDEVGLLAREVPGAVVVEVHRGKSRLFCWRDHALRIEPGDRVL
                     AIDGSGPT"
     gene            complement(1344..1601)
                     /locus_tag="VD737_08870"
     CDS             complement(1344..1601)
                     /locus_tag="VD737_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10714.1"
                     /translation="MPAQISPEEVLALLFPEPARGGQPAQTVTAFVRDPMGGLMLQTV
                     VPNRSLLKAALVAGRANDGTPIVDPVTREVIGYELAPLAVA"
     gene            1832..2596
                     /locus_tag="VD737_08875"
     CDS             1832..2596
                     /locus_tag="VD737_08875"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HXW10715.1"
                     /translation="MTSDDARLPFLIAHRGNAADYPENTLAALRSAIGLGLRHVEFDV
                     QLTRDGVPVVLHDADLRRMTGRVECVHDLAWSELAQVPLTEPGRFGDRHAELRVSTLA
                     EVLAALQQWPDVTAFVEVKRASLRRFGHDAVLERVAGTVANHLQACVLISFDLPCVER
                     LRAMTGARIGWVIEQYDDATRVRLQDLSPDYVFADVEGLPREADARLWSGPWCWAIYE
                     VRDLETARRCARLGAHAVETMHVRAMLDAYARACAP"
     gene            2593..4149
                     /locus_tag="VD737_08880"
     CDS             2593..4149
                     /locus_tag="VD737_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HXW10716.1"
                     /translation="MTLGGSDDDLAWLAGELSAIRRELLPAEESRVLHLALDQGGSSS
                     RAVLFDAVGREVASAHVPIHTRRDGDRVEHDADELAQSLVTAARDACDLPLAAGRPVV
                     VAGLATQRSTVCCWDATDGAALSPAISWQDRRHAAWLAQALGARAEWVRELTGLPLSA
                     HYGASKLRWCLDELPAVRLALRDERLAMGPLASFLLHRLCIERPLFADPANASRTLLY
                     DPAVLDWSAPLLEAFELPRSALPRCVGTTHAFGTVTLDERRRVPLRACSGDQSAAVFA
                     FGTPTPGTCFVNVGTGAFVQRIAHDRASPPPRGLLKSVVQARTDDVHGAAYVHEGTVN
                     GALAAVEWLRGRVGIDVTRALATLRHALPPDQVELLFMNGVGGLGAPWWLPSFPVEFV
                     SLDGAPPRASVGELQQLAAIVDSVAFLIAVNVDGLHRGAPLERLVVTGGLAGCDYLCE
                     VLADVTRLKVERPALREATARGIAFLAAGQPDHWQPVPLERTFSPAGRPRVLARYARW
                     REEMGKRGAG"
     gene            complement(4276..5304)
                     /locus_tag="VD737_08885"
     CDS             complement(4276..5304)
                     /locus_tag="VD737_08885"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXW10717.1"
                     /translation="MTDASVLPTGSDPGFAPPRRFRHHHLQSIYPSLPIRRRTVERRC
                     APLLGAARTEILDCGDGVRLLAQVTTQVRLGRAPSDKLAVLLHGWEGSAQSLYLLSLG
                     QTLLEAGYDVARLNLRDHGDSHHLNEEIFHSCRIGEVVGAVRSLQSIHAGQRLCLAGF
                     SLGGNFSLRVGARAAEAGIDVSRIVAVCPVLDPEHTLQRLESGWALYREYFVWKWRRS
                     LRRKQRAWPSVYESLEDILRLGNLTAMTEQLACRYGGFATLQDYLRGYAIVGPVLEPL
                     ALIPNARCRIIAAADDPICPASDLERVARPPNLEITVTRFGGHCGFYDGRPGPSWVER
                     EVVRTFAD"
BASE COUNT          825 a         1883 c         1910 g          807 t
ORIGIN      
        1 tgaaggcgac gagggcggcg atcgcagatc gagccctgcg ccaattgata gccataagtc
       61 ctcgcggacg aattgcggca gcggtgcgcc gagtcaacgc gattcttctc ctggtttgag
      121 cactcgtcag gtaggcgatt catcacctgg atccaacgac cgcactgggg tcgcgccgcc
      181 aagccgggct gaccaccctt ctgggcctca ggtgggtccg ctgccgtcga tggcgagcac
      241 gcggtcgccg ggctcgatgc gcagcgcgtg gtcgcgccag cagaacaggc ggctcttgcc
      301 acggtggacc tccaccacga cggcacccgg cacctcgcgt gccagcaggc cgacctcgtc
      361 gggccgcacg tcacgctcga ccagctggta atcgccgcgg tacgacagca actcggagac
      421 gaagtccacc gtcccgtgcg tcgccacggc gtccgccatc aggaagccgc cgaaccgcgg
      481 cggcgagacc acttcgtcgg caccgcccgc gcgcacgagc ttgatgttct cgggctctcg
      541 cacgctcgcg atgatgcgta cctgcttggt gagcgagcgc gccgtcagta cgaccagcac
      601 cgtcgtgtcg tcgcgaccca cgctgacgag cagcgactga gcctcgcgca gtcccgccat
      661 cctcatcagc tcttcgctcg aggcgtcgcc gtggatgccg atcagcccgc gctcggccgc
      721 cttggccact tcctcacggt cgcggtcgat cacgacgacg cgctcgccgt cccagccacg
      781 gagcagcagt tcgttcgcgg cgatcgaccc gctgtgcccg tagccgcaga tgatcacgtg
      841 tcccttgagc tcgcgctgca gtcgcaacat gcgccattcc tcgaagaaac gttgcagcac
      901 gagctggtag gccgtgccga ggaagaggag ccagatgaac atccgcaccg gcgtgacgaa
      961 gaacgcgtcg atgagccgcg cctggtcgga gacgggaacg atgtcgccat agccgaccgt
     1021 cgtgaccgtc accatcgtga aatagaacac gtccgagacc gaaacatgcc cgtcgatgtt
     1081 gtcctgcagg cccgcgcgat cgatcaggaa gaccaggaac accgacgcga tcagcagcag
     1141 gaccagaccc gcgcgcagca gcagcgtgac ttccggtggc gtcgtcgccc gacgcaacag
     1201 gatcggcggc gcggcgtcgc cacgttcggc acgcatgcgc cgcatcgcgc cccggaccca
     1261 ccggaagcgc aggttcgacg gtctccgcat gcggagacta tagcgcagcg tcgggggcgg
     1321 tcagctcgcc ggacagggac ggatcaggcg accgcgagcg gggcgagttc gtacccgatc
     1381 acctcgcggg tcacgggatc gacgatcggc gtgccgtcgt tcgcacgccc tgcgacgagc
     1441 gcggccttga gcaggctgcg attcggcacg acggtctgca gcatcagccc gcccatcgga
     1501 tcgcgcacga aggcagtgac cgtctgcgcc ggctgcccgc ctcgggcggg ttcgggaaac
     1561 agcagggcga ggacttcttc gggtgagatc tgtgcgggca tcgatcgtgg tgtccgtcgt
     1621 ggtcgtgacg tctcctgcac ggttgcgcag caagcctcgt gccatgcgcg agggccgcga
     1681 aatgcgaccg attcgtccgg ccggcaacga cttcgattga cacgaggggt ccatcgacgg
     1741 acacgacgtg tccactcgtg acgcgcatca cacggccgcg ttgagtaaga tcgccggcgg
     1801 tccgggcgcg gcccgaccga gcggaacgcc catgacctct gacgacgccc gattgccttt
     1861 cctgatcgcc catcgcggca acgccgcgga ctatcccgag aacacgctcg cggccctgcg
     1921 gtcggcgatc gggctgggat tgcgccacgt cgagttcgac gtgcagctga ctcgcgacgg
     1981 cgtgccggtc gtgctccacg acgccgacct gcgccgcatg acgggcaggg tcgagtgcgt
     2041 gcacgacctc gcctggagcg agctggcgca ggtgccgctc accgagcccg ggcgattcgg
     2101 cgatcgccat gcggagctgc gcgtgtcgac gctggctgag gtgctcgcgg ccttgcagca
     2161 gtggcccgac gtcaccgctt tcgtcgaggt caagcgcgcg agcctgcggc gtttcggtca
     2221 cgacgccgtg ctcgaacgcg tcgcgggcac ggtggcgaac cacctgcagg cctgcgtgct
     2281 gatttcgttc gacctgccgt gcgtcgagcg gctgcgggcg atgaccggcg cccgcatcgg
     2341 ctgggtgatc gagcagtacg acgacgcgac gcgcgtacgg ctgcaggacc tctcgcccga
     2401 ctacgtattc gccgacgtcg agggcctgcc acgcgaggcg gacgccaggc tgtggtcggg
     2461 gccgtggtgc tgggcgatct acgaggtacg tgacctcgag accgcgcgac gctgcgcccg
     2521 acttggcgcg cacgcggtcg agacgatgca cgtgcgcgcg atgctcgacg cctacgcaag
     2581 ggcctgcgcg ccgtgaccct cggcggttcc gacgacgacc tggcgtggct ggccggtgaa
     2641 ctgagcgcca tccggcgcga actgctgccg gcggaggagt cgcgcgtgct gcacctcgcg
     2701 ctcgatcagg ggggcagctc gagtcgtgcc gtgctgttcg acgcggtcgg acgtgaagtg
     2761 gcgtccgcgc acgtgcccat tcacacgcgg cgcgacggcg atcgcgtcga acacgacgcc
     2821 gacgaactgg cgcagtcgct ggtcaccgcg gcacgcgacg cctgcgacct gccgcttgcg
     2881 gccgggcgcc ccgtggtcgt cgcgggactc gccacccagc gctcgaccgt gtgctgctgg
     2941 gacgcgaccg acggtgccgc gttgagcccg gcgatctcct ggcaggaccg gcgacacgcc
     3001 gcctggctcg cgcaggcgct cggcgcgcgg gcggagtggg tccgcgaact gacgggcctg
     3061 ccgctgtcgg cgcactacgg cgcgagcaag ctgcgctggt gcctcgacga gctgccggcc
     3121 gtgcgcctgg cgctgcgcga cgagcggctg gcgatggggc cgctcgccag cttcctgctg
     3181 catcggctct gcatcgagcg tccgctgttc gcggacccgg ccaacgcttc gcgcacgctg
     3241 ctctacgatc cggccgtgct cgactggtcg gcgccgttgc tcgaggcgtt cgagctgccg
     3301 cggtcggcgc tgccgcgctg tgtcggcacg acccacgcgt tcggcaccgt gacgctcgac
     3361 gaacgacgac gcgtgccgct gcgcgcgtgc agcggcgacc agtccgccgc ggttttcgcg
     3421 ttcggcacgc ccacccctgg cacgtgcttc gtcaacgtgg ggaccggcgc gttcgtgcag
     3481 cgcatcgcgc acgaccgggc gtcgccaccg ccgcgcgggc tgctcaagag cgtcgtccag
     3541 gcgcggaccg acgacgtaca cggcgcggcg tacgtacacg agggcacggt caacggcgcg
     3601 ctcgccgccg tcgagtggct gcgtggccgt gtcgggatcg acgtgacgcg cgcgctcgcg
     3661 acgctccgcc acgcactgcc gccagaccag gtcgagctgc tgttcatgaa cggcgtcggc
     3721 gggctcggcg caccctggtg gctgccgtcg ttccctgtcg aattcgtgtc gctcgacggc
     3781 gcgccgcctc gcgcgtcggt cggcgaattg cagcagctcg cggccatcgt cgacagcgtc
     3841 gccttcctga tcgcggtcaa cgtggacggc ctgcatcgcg gcgcgccgct cgagcgcctg
     3901 gtcgtcaccg gtggactggc gggctgcgac tacctttgcg aagtgctggc cgacgtgacc
     3961 cgcctgaagg tcgagcgacc ggcgctgcgc gaggcgaccg cccgcggcat cgcgttcctc
     4021 gccgccggac agccggacca ctggcagccc gtgccgctcg agcgcacgtt ctcgccggcc
     4081 ggccggcctc gcgtactcgc ccgctacgcg cgctggcggg aggaaatggg caagcgggga
     4141 gcgggatgac cagtgattag tggctggtgg ctggtggctg gtggctagtg gctagtggct
     4201 agtggctagt ggtcagacaa gaacaagaca tcgaagcacc gtcctcacta gccactgacc
     4261 actaaccact gaccactagt cggcgaaggt ccgcaccact tcccgctcca cccacgacgg
     4321 gccgggacga ccgtcgtaaa aaccgcagtg gccgccgaaa cgcgtcacgg tgatctcgag
     4381 gttcggggga cgtgcgacgc gctcgaggtc ggacgccggg cagatcgggt cgtcggccgc
     4441 ggcgatgatg cggcagcgcg cgttggggat aagcgcgagc ggctcgagca ccggcccgac
     4501 gatcgcatag ccgcgcaggt aatcctgcaa cgtcgcgaag ccgccgtagc ggcaggcgag
     4561 ctgctcggtc atggcggtga ggttgcccag ccggaggatg tcctcgagcg actcgtagac
     4621 gctgggccag gcgcgttgct tcctgcgcag cgaacggcgc catttccaga cgaagtactc
     4681 gcgatacagc gcccagccgg actcgagccg ctgcagcgtg tgttccgggt cgagcacggg
     4741 acagaccgcg acgatccgcg agacgtcgat gccggcctcc gccgcgcggg cgccgacccg
     4801 caggctgaag ttgccaccga gcgagaatcc cgccaggcac agccgctgcc ccgcgtggat
     4861 cgactgcagg ctgcgcacgg cgccgaccac ttcgccgatg cggcaggagt ggaagatctc
     4921 ctcgttcagg tgatgactgt cgccgtggtc gcgcaggttc agtcgtgcca cgtcgtagcc
     4981 ggcctcgagc agcgtctgac caagcgacag caggtacagc gactgtgcgc tgccctccca
     5041 gccgtgcagc agcacggcca gcttgtcgct cggggcacga cccaggcgca cctgcgtggt
     5101 gacctgggcg agcagccgga caccatcacc gcagtcgagg atctcggtcc gtgccgcgcc
     5161 gagcagcggc gcgcatcggc gctcgaccgt gcggcgccgg atcggcaggc tcggatagat
     5221 cgactgcagg tggtgatggc ggaaccggcg tggcggcgcg aaccccggat ccgagcccgt
     5281 cggcaaaaca ctcgcgtcgg tcatgagtgc cggacgcggt cccccgctcg agcgtcagtc
     5341 atgggagttt actcaggcgg cggggaccag gtggaattcg acgcgattgt tctgcgcggc
     5401 cacgacgttc caggcgctcg cggtc
//
LOCUS       DASPFR010000339         3822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1810328, whole genome shotgun sequence.
ACCESSION   DASPFR010000339 DASPFR010000000
VERSION     DASPFR010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810328"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(449..1858)
                     /locus_tag="VD737_08890"
     CDS             complement(449..1858)
                     /locus_tag="VD737_08890"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXW10718.1"
                     /translation="MGSGTAEVARADASSTSTGGVNEWPDQRYGWFVVGVLTLAYTCS
                     FIDRQILTLLIEPIRRDLQISDTQVSLLGGLAFSILYTTLGIPIARLADQAHRRNLMA
                     AGLAFWSLMTAACGVARGFWGLFAARVGVGVGEAALSPAAFSLMADYFPPRKVALAIS
                     VYSTGLYFGAGLALMIGGTVVQLVSNAPTHDLPIVGTVFPWQLTFFVVAAIGLPVLLL
                     MLLIREPLRRGVTTAGTAPGAASAWPALRAFMRANRRTLACHISAFTLFGIGINCYLF
                     WAPTLMMRTHGWTAPQAGFTIGAMLFGLGTAGVYGGGWLADRLAGRGHRDAVLRAAFY
                     GMLAGSPFLIATPLLDDARLATIGLGISVFFMAFPQGLPSAALQVVTPNRLRAQMTAI
                     YFFIGNLIASGLGPTLPALLGDYVFRDPSMLRYGLFVVQAIVMPASLLCLWLGFSAYR
                     RSVEQAEAREQPPPHVAAL"
     gene            complement(1861..2730)
                     /locus_tag="VD737_08895"
     CDS             complement(1861..2730)
                     /locus_tag="VD737_08895"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HXW10719.1"
                     /translation="MPVDPAKLMALRLPDHEFGWTQRDCQLYALATGFGQDPVDEREL
                     RYVLEGPGFQVSPSAAVTLYYDDRWMRASGVDLAMSLHGEQRQVFHRAIPADGRGRVS
                     SRITGIYDKGPGRGLLVVCEQVLRDASTDEPIATNVITNFARADGGIGWSSGPAPQPH
                     ALPARAPDAVVEGRTRPEQALLYRLCGDRNPLHADPATARAAGFARPILHGMCTYGFA
                     ARAILQVACDYDARRLRGLGARLSAPVFPGDVLRTELWIDGDVVSFRTSVPEREAVVL
                     NNGRAEFAPPSTG"
     gene            2859..>3822
                     /locus_tag="VD737_08900"
     CDS             2859..>3822
                     /locus_tag="VD737_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXW10720.1"
                     /translation="MSSRSATRVRAKKPPSGKPDAGRPVTAPAEPAGPEAARLAGIAA
                     QVGPERAGAILRGSQVAMAFEAVGDRWSALVMREVFLGARRFEELVSATGASRATLTL
                     RLRSLVDEGILHRHPYQTRPTRYEYRLTRMGADLYPSALMYWLWERRYGGTSDLPQEL
                     LHRTCGQSMLPLLICRTCTQPITINDVRVEVVAEPDERQVSLPKHCLHTGPDSWREAD
                     GRAVHVIDVIGDRWTALVQASTYYGLHRFADIQAALAVPTNTLADRLRLLVHAGVFQR
                     TQYHEHPPRYAYRLTDKGRALYLPAFAMHQWAERWLLRGRVAPIR"
BASE COUNT          630 a         1347 c         1334 g          511 t
ORIGIN      
        1 catacgacac tcctccccac agggggatca gtgttgcaac gaccggttga gaccgccccc
       61 tggctcgcga cggtctgccc gatcccgtcc ggaccgggca cgccctgcgt cggcgatgac
      121 tcaaacgcgc tcgcagtcgt cgccgactca cgctcggggg actggacgga gttctcgcgg
      181 acgccgaccg cgcgctctga atcacgtctc agcgcgcgct aaccgccgcg agggttgccg
      241 ccggatttcg agccgggggg cgtcacgagc cagggatggc gagtgcgagc cggccaggat
      301 ggcctcttgc tggcgtcccc ccggatcgaa atgcggcggg cagccctcag cggccgctgt
      361 tacgtggccg cccccaaatg cagcgggcag ccctcagcgg ccgctatcac gtggccgccc
      421 gaagtgcggc ggtcagttct ccacgcagct acagcgctgc gacgtggggc ggaggctgct
      481 cgcgcgcttc ggcctgctcg acgctgcggc gatacgcgct gaaaccgagc cacaggcaca
      541 gcaggctcgc cggcatcacg atcgcctgca cgacgaacag gccgtaacgc agcatcgatg
      601 gatcgcggaa cacgtagtcg cccagcagcg ctgggagtgt cggcccgagt ccgctcgcga
      661 tcaggttgcc gatgaagaag tagatggccg tcatctgcgc gcgcagccgg ttcggcgtga
      721 cgacctgaag tgccgccgac ggcaggccct gaggaaacgc catgaagaac accgagatgc
      781 cgagcccgat cgtggcgagg cgcgcgtcgt cgagcagcgg cgttgcgatc aggaatggtg
      841 agcccgcgag catgccgtag aacgcagcgc gcagcaccgc gtcacgatgc ccacgacccg
      901 ccagtcgatc ggccagccag ccgccgccgt acacgccggc cgtgccgaga ccgaacagca
      961 tcgcgccgat cgtgaacccg gcctgcgggg cggtccagcc gtgcgtgcgc atcatcagcg
     1021 tcggcgccca gaacaggtag cagttgatgc cgatgccgaa cagcgtgaac gcgctgatgt
     1081 ggcaggccag cgtgcgccgg ttcgcgcgca tgaacgcgcg cagcgccggc caggcgctcg
     1141 cggcccccgg tgccgtgccc gccgtcgtga cgccgcggcg caagggctcg cggatcagca
     1201 acatcagcag caagaccggc aggccgatgg cggcgacgac gaagaacgtc agttgccacg
     1261 ggaagacggt gcccacgatc ggcaggtcgt gcgtcggcgc attcgagacc agttgcacga
     1321 ccgtgccgcc gatcatcagc gcgagcccgg caccaaagta caggccggtc gagtacacgc
     1381 tgatcgccag ggcgaccttg cgtggcggga agtagtcggc catcagcgag aacgcggccg
     1441 gcgacagggc ggcctcgccg acacccacgc cgacgcgggc cgcgaagagt ccccagaacc
     1501 cgcgggccac gccacacgcg gcggtcatca aactccagaa cgccaggcct gccgccatga
     1561 ggttgcgccg gtgcgcctgg tcggccaatc gcgcgatcgg gatgccgagc gtcgtgtaga
     1621 ggatcgagaa cgcgagaccg cccagcaggc tcacctgcgt gtcgctgatc tgcaggtcac
     1681 gacggatcgg ctcgatcagc agcgtcagga tctggcggtc gatgaacgag cacgtgtacg
     1741 cgagcgtcag cacgccgacc acgaaccagc cgtaccgctg gtccggccac tcgttcacgc
     1801 cgcccgtgga ggtcgacgac gcgtcggccc gtgcgacctc ggccgtgccg gaacccatcg
     1861 tcagccggtg gacggcggcg cgaactccgc gcgtccgttg ttgagcacga ccgcctcgcg
     1921 ctccggtacc gaggtgcgga acgagacgac gtcgccgtcg atccagagtt ccgtccgcag
     1981 gacgtcgccg gggaacacag gggcactcag tcgggcaccc aggccgcgca ggcgccgtgc
     2041 atcgtagtcg caggccacct gcaggatggc acgcgccgcg aagccgtacg tgcacatgcc
     2101 gtgcaggatg ggtcgggcga aacccgccgc gcgcgccgtg gccgggtcgg cgtgcagcgg
     2161 attgcggtcg ccgcacagcc gatagagcag cgcctgttcc gggcgcgtgc ggccctcgac
     2221 caccgcgtcg ggcgcgcgtg ccggcagcgc gtgaggctgc ggagcagggc cgctcgacca
     2281 gccgatgccg ccgtcagcgc gggcgaaatt ggtgatgaca ttggtcgcga tgggctcgtc
     2341 cgtgctggcg tcgcgcagca cctgttcgca gacgacgagc aggcctcgac ctggaccctt
     2401 gtcgtagatg ccggtgatcc gggaggagac gcggccgcgg ccatcggccg gaatggctcg
     2461 gtggaacacc tggcgctgct cgccgtgcag cgacatcgcg aggtcgacgc cactcgcgcg
     2521 catccagcgg tcgtcgtagt acagcgtgac ggcggcgctc gggctcacct ggaagccggg
     2581 tccctcgagg acgtagcgga gttcccgctc atcgaccggg tcctgcccga atccggtggc
     2641 gagcgcgtag agctggcagt cgcgctgcgt ccagccgaat tcgtggtcgg gcagccgcag
     2701 cgccatcagc ttcgccggat cgacaggcat gggacaccca ctcactgaga ggcacgtcca
     2761 gcatacgagt tcagaatggc aagtgacaag ccgaccggcg ccggcatcgc ggtgtatacg
     2821 ggtggtgcaa tccggatgcg gtcactatag tgccgcgaat gagctcccga tcggcgacgc
     2881 gagttcgcgc gaaaaagcca ccgtccggca agcccgacgc tggcaggccg gtcactgcac
     2941 cggccgaacc tgccggcccc gaagccgcgc gactcgccgg catcgcggcg caggtcggcc
     3001 ccgagcgcgc cggtgcgatc ctgcgcggca gccaggtcgc gatggccttc gaggcggtcg
     3061 gcgaccgctg gtcggccctg gtcatgcgcg aggtgttcct cggcgctcgc cgtttcgagg
     3121 aactggtgtc cgcgacgggc gcgagccgcg ccacgctgac gctgcggctg cgctcgctcg
     3181 tcgacgaggg catcctgcac cgccatccgt accagacgcg ccccacccgc tacgagtacc
     3241 ggctcacccg catgggcgcc gacctctacc cgagcgcgct gatgtactgg ctctgggagc
     3301 ggcgctacgg cggcacgtcc gacctgccgc aggaactgct ccatcgaaca tgcggccagt
     3361 cgatgctgcc gctgctgatc tgccgcacct gcacgcagcc gatcacgatc aacgacgtcc
     3421 gcgtcgaggt ggtcgccgag cccgacgagc gccaggtgag cctccccaag cactgcctgc
     3481 acaccggccc ggattcctgg cgtgaagcgg acgggcgcgc cgtgcacgtg atcgacgtga
     3541 tcggcgaccg ctggacggcc ctggtgcagg cgtcgacgta ttacggcctg cacaggttcg
     3601 ccgacatcca ggccgcgctc gccgtgccga ccaacacgct ggcggaccgg ctgcggctgc
     3661 tcgtccacgc aggcgtcttc cagcgcacgc aataccacga acacccgccg cggtacgcgt
     3721 accgcctcac cgacaagggc cgggcgctct atctgccggc gtttgcgatg caccagtggg
     3781 ccgagcgttg gctgctgcgc gggcgagtcg cgccgatccg tc
//
LOCUS       DASPFR010000340         7284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_102799, whole genome shotgun sequence.
ACCESSION   DASPFR010000340 DASPFR010000000
VERSION     DASPFR010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102799"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VD737_08905"
     CDS             complement(<1..629)
                     /locus_tag="VD737_08905"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HXW10721.1"
                     /translation="MSAPDRMDLLLLHAPSVYDFREHSILYGPVSDMVPSSTVFEMYP
                     LGFMTLAAWLQDRGFDVRIVNLALRMIRDRRFDVPRFLGGLAPRAIGIDLHWLPHAHG
                     ALEVARLCKECHPDVPVIMGGLSASYFHEELVRYPQVDCVLRGDSTEAPLQRLLEALR
                     RGDPLADVPNLTWKERGEVRVNSHRYLPDTLDYVDLRPEVLARMVLRHR"
     gene            complement(626..1585)
                     /locus_tag="VD737_08910"
     CDS             complement(626..1585)
                     /locus_tag="VD737_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HXW10722.1"
                     /translation="MSADRTPDAVAGTLAADTRVHVFLGKGGVGKTTCAAATALALAE
                     AGESSLVISTDPTPSLSHIFEVEGLHRDRQVLPGLCMTEFGLEDVRAMWDEKFGREVY
                     GVFSSFVDVDYPTFVQFMSSVLPGLNEEFMVDYVRRLALDARYRNVVWDTAPLGQTLA
                     LLGMPAMLDEHLRTAPRIYSRLRTSGDRREPVLAIIGRWRRLAADCMEFLRRDVSFSM
                     VTIPEALSVCQLDGVVRELARYGLRFDRVVVNNVVQASDSALLERRVRQQRPHLERIR
                     AQFGHPGVVHVPWFPEEVRGLERLRAAGRCLCGTPAVAALAVP"
     gene            complement(1693..2556)
                     /locus_tag="VD737_08915"
     CDS             complement(1693..2556)
                     /locus_tag="VD737_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXW10723.1"
                     /translation="MNLRVLGIALLSSSLPGLPAQAVAGSSPAHVTVSQAARPDVRSQ
                     SYYILDGTNSAVLASRHENTPAPIASITKLMTALVVLEADQPMDEELAITAADVRGTA
                     GSGSRLWQGARMTRADLLRLALMSSENRAAHALCRAYPGGSTACVRAMNAKAAGLGMK
                     TAHFIEPTGLSKLNVASPRDLARLVLAAGAVPTIGEYSTDPEHTVYVGRQALQFRNTN
                     SLVEKPDWNVGVQKTGYISEAGRCLVMQTVIDGRQVVIVLLNSFGRLTRIADAKRIRT
                     WLEAQHKARDA"
     gene            2806..4296
                     /locus_tag="VD737_08920"
     CDS             2806..4296
                     /locus_tag="VD737_08920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXW10724.1"
                     /translation="MLNRRGFVGLSLAAAAGATLPIARVLAEALPLAEVRAVTREGAA
                     TTIAASDLQHLRQSLRGPLLLPGDAGYDTARRVINESIDKHPALIVQPTGAADVRTAV
                     DFARQHNLLLAVKCGGHSYAGKSTCDGGMQIDLSRMRGVRVDPAARVAYVSGGSLLGE
                     IDHEAMALGLVTTAGTVSHTGVGGLTLGGGFGRLARRFGLACDNLRSVDVVTADGRLL
                     HASERENPDLFWGVRGGGGNFGVVTQFEFALHPMQRQVIGGPLVFPIDRARELMLAYA
                     EITAAAPPELYLDAILTTPRGGKPGMFVFDSCYSGPAAEAERAFAPLRKLGTPIADRV
                     RPVDYVAIQRTNDRTDPRNEGMYLKSGFIDAFPEEIARTLVEGYEPHPERGTTVFFQH
                     AGGAIGRVPAAATAYPHRRATHNMFALVSWPLTADATPHVKYIKSYWATLERHTNGYY
                     TNEVADEGQNVIDDNYQGNLPRLRQVKAKYDPNNLFRLNANVKPMA"
     gene            complement(4336..5973)
                     /locus_tag="VD737_08925"
     CDS             complement(4336..5973)
                     /locus_tag="VD737_08925"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HXW10725.1"
                     /translation="MIRIAANATWPLVVLAVASLAGCGGGGDSSGGGGGNPPPPGEPA
                     IFQTATAVRVSRATPFAGGCLAVPAGSTVYTNAEVEPHLAVDRTNPNHVVAAWQQDRL
                     SDGGARGLATAVSIDGGLTWDAPQPAPFSTCAGGAFARVSDPWVAVVGNTVLQIGIAF
                     TGAANSVGARSAVAVSRSVDGGVTWGPGVLLVDDDGNRLFNDKEALSIDTTDPRYVYA
                     VWDRIGLDERGPTLLARSIDGGASWEPTRTIYDPGAGRQTIGNVPVTTPDGIVHVFFV
                     ELGPDPANASGLEAHVSVIRSSDKGATWSVPTRIAEARPVGTVTPQGGQPVRSGEILG
                     TFAADPDDGTLYAAWQDSRFSSGARDAIAVSQSSDGGLTWTAPSRVNARADVPAFTPT
                     LAVLPGGAIGVTYYDFRQAGTATYQPTDFWLASSSNGVDWAETRLAGNFDMFDAPDAG
                     GLFVGDYQGLDAANASTFLAVYSRTNNGEAANRTDVYADRVPVITGAVASSPVDAAAM
                     KTAPWSAEAQARVSRHLVATREARLERWRAWRESPPQ"
     gene            6359..6784
                     /locus_tag="VD737_08930"
     CDS             6359..6784
                     /locus_tag="VD737_08930"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HXW10726.1"
                     /translation="MTTLARDICLTELDAVRLESMLDQVRRRAPEERSSIEALEERVD
                     AARVVHQSCIDPQVVTMNSVVALTDRGTGETVELTLAYAAEADAEQGRVSVLSPVGRA
                     LIGARIGDVLRVAVPAQRDKTFEVSAIPYQPEAQGRYDL"
     gene            complement(6810..>7284)
                     /locus_tag="VD737_08935"
     CDS             complement(6810..>7284)
                     /locus_tag="VD737_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10727.1"
                     /translation="TPELRSSVLRASRDGCLRVFTDPRASPTGFPFKVVRLPGTVSEP
                     DLYAARERICDLAYLRQCYKTEDGTVGYRCPGEPLEDFLRKGGTEAETAGRKCVCNGL
                     VSTVGFEQRRRDGGLEPPLVTAGDDVTDLQRFVRPGRDGYSADDVIDTLLGRRAA"
BASE COUNT         1131 a         2545 c         2574 g         1034 t
ORIGIN      
        1 tcgcggtgcc gcaggaccat ccgcgcgagc acctccgggc gcaggtccac gtagtcgagc
       61 gtatcgggca ggtagcggtg cgagttgacg cggacctcgc cgcgctcctt ccacgtcagg
      121 ttcggcacgt ccgcgagtgg atcgccgcgt cgcagcgcct cgagcagccg ttgcagcggc
      181 gcctccgtgc tgtcgccccg caggacgcag tcgacctggg gatagcgcac gagctcctcg
      241 tggaaatagc tcgcggagag cccgcccatg atcacgggga cgtccgggtg gcactccttg
      301 cagaggcgcg ccacctcgag cgcaccgtgc gcgtgcggca gccagtgcag gtcgatgccg
      361 atcgcgcgag gcgcgagccc gcccaggaac cgcggcacgt cgaagcgacg gtcccggatc
      421 atgcgcagcg ccaggttgac gatgcgcacg tcgaaaccgc ggtcctgcag ccacgccgcg
      481 agcgtcatga aaccgagcgg gtacatctcg aacaccgtcg acgagggcac catgtcgctg
      541 acgggcccat agaggatcga gtgctcgcgg aagtcgtaca cgctcggtgc gtgcagcagc
      601 agcaggtcca tgcggtcggg cgcgctcacg gcacggccag cgccgcgacc gccggcgttc
      661 cgcacaggca gcgacccgcc gcccggagtc gctcgagacc gcgcacttcc tcgggaaacc
      721 agggcacgtg cacgacgccc gggtggccga actgcgcccg gatccgctcc aggtgcggcc
      781 gctgctggcg cacgcgccgc tcgagcaacg ccgagtcgga cgcctgcacc acgttgttga
      841 cgacgacacg gtcgaacctg agcccatagc gggccagctc gcgcaccacg ccgtcgagct
      901 ggcagacgct gagcgcctcc ggaatcgtca ccatcgaaaa cgagacgtcc ctgcgcagga
      961 actccatgca gtcggccgcg agccgacgcc agcgaccgat gatcgcgagc accggctcgc
     1021 ggcggtcccc cgacgtgcgc agccgcgagt agatccgcgg cgccgtgcgc aggtgctcgt
     1081 cgagcatcgc cggcatgccg agcagcgcca gcgtctggcc gagcggtgcg gtgtcccaga
     1141 cgacgttccg gtagcgcgcg tcgagcgcga gccgccggac gtagtcgacc atgaactcct
     1201 cgttgagccc gggcagcacc gagctcatga actgcacgaa cgtcgggtag tcgacgtcca
     1261 cgaagctcga gaacactccg tagacttcgc ggccgaattt ctcgtcccac atggcacgga
     1321 cgtcttcgag gccgaactcg gtcatgcaaa gcccgggcag cacctggcga tcgcggtgca
     1381 gtccctcgac ctcgaagatg tgtgacagcg agggcgtcgg atccgtcgag atgacgaggc
     1441 tggattcgcc cgcttcggcg agcgcgagtg ccgtggccgc agcacaggtc gtcttgccga
     1501 caccgccctt gccgagaaag acgtgcacgc gcgtgtcggc ggccagcgtg ccggcgactg
     1561 cgtcgggcgt gcgatcagcg ctcatggcct cgatgacctg ggacgtgccc gtcgatcatg
     1621 gcaccccgag cgcctcgccg aatcagtaac ggcgcttatg gacctcgcgc aatctgcgcg
     1681 agcgcagcgc cctcaggcgt cccgagcctt gtgctgggcc tcgagccagg tccggatgcg
     1741 cttcgcgtcg gcgatgcgcg tgagccggcc gaacgaattc agcagcacga tcacgacctg
     1801 gcgaccgtcg atcacggtct gcatgaccag gcagcgaccc gcttccgaaa tgtagcccgt
     1861 cttctgcacg cccacgttcc agtccggctt ctccaccagc gagttggtgt tgcggaactg
     1921 cagggcctgc cgccctacgt agacggtgtg ctccgggtcc gtcgagtatt cgccgatggt
     1981 cgggaccgcg ccggcggcca gcacgagcct ggcgaggtct cgggggctcg ccacgttgag
     2041 cttggacagg cccgtcggct cgatgaagtg tgcggtcttc atgccgagcc cggccgcctt
     2101 ggcgttcatg gcacgcacgc aggctgtcga tccgccgggg taggcgcggc acagggcatg
     2161 tgccgcccgg ttctccgagg acatcagcgc caggcgcagc aagtccgccc gcgtcatgcg
     2221 ggcgccctgc cagagtcgcg agcccgaacc cgccgtgccg cggacgtcgg ccgccgtgat
     2281 ggccagttcc tcatccatgg gctggtccgc ctcgagcacc accagcgccg tcatcagctt
     2341 ggtgatcgag gcgatcggcg ccggcgtgtt ctcgtggcgc gaggccagca cggcggagtt
     2401 cgtgccgtcc aggatgtagt acgactgcga gcgcacgtcc gggcgtgccg cctgcgagac
     2461 cgtgacgtgc gccggactgg acccggcaac ggcctgggcg ggcaggcccg gcagcgagga
     2521 cgacagcagc gcgatgccga ggacccgtag gttcattctt gcttgtccgc ctgtgactcg
     2581 acgtgcttcg aacgccccgg gccaccggca cggagccttg tttcaccgca cgagcgtagt
     2641 cggcggaccg aagccgggtg tgtgaccggc gccgcagaat ctagcggcga ggcaaggccc
     2701 cggggcgcag atgccaccgg aacggcaacg tttctggaag cgcgcgggca ggagtaagct
     2761 cgcgcaaaac gaggccgcca gacggcacga ccgggaggaa gaacgatgct caatcgacgt
     2821 gggttcgtgg gactgtcctt ggcggccgcg gccggcgcca cgctgccgat cgcacgcgtg
     2881 ctcgcggagg cgttgccgct cgccgaggtg cgtgccgtca cgcgcgaagg cgcggctacg
     2941 acgatcgcgg ccagcgacct gcaacatctg cggcaaagcc tgcgcggacc gttgctgctg
     3001 cccggcgacg cgggctacga caccgcacgc cgcgtgatca acgagtcgat cgacaagcac
     3061 cctgcgctga tcgtgcagcc gaccggcgca gccgacgtgc gcacggccgt cgattttgcg
     3121 cggcagcaca acctgctgct cgcggtgaag tgcggcggcc acagttatgc cggcaagtcc
     3181 acctgcgacg gtggcatgca gatcgacctc tcccgcatgc gcggcgtccg ggtcgatccg
     3241 gcggcacggg tcgcgtacgt gtccggcggc agcctgctgg gcgagatcga tcacgaggcg
     3301 atggcgctcg ggctggtgac cacggccggc acggtctcgc acacgggcgt cggcgggctc
     3361 acgctcggcg gagggttcgg gcggctggcg cgccggttcg gactcgcgtg cgacaacctg
     3421 cgttccgtgg acgtcgtcac ggccgacgga cgcctgctgc acgccagcga gcgcgagaac
     3481 cccgacctgt tctggggcgt gcgcggcggt ggcggcaatt tcggggtggt cacccagttc
     3541 gagttcgcgc tgcatccgat gcagcggcag gtgatcgggg ggccgctcgt gttcccgatc
     3601 gatcgagcgc gcgaactgat gctggcgtat gccgagatca ccgctgcggc gccgcccgag
     3661 ctgtacctgg acgccatcct gaccacgccg cgcggcggca agccagggat gttcgtcttc
     3721 gacagctgct actccgggcc ggccgccgag gcggaacggg cgtttgcgcc gctgcgcaag
     3781 ctcggcacgc cgatcgcgga ccgggtgcgg ccggtcgact atgtcgcgat ccagcgcacc
     3841 aacgaccgca cggatccacg caacgagggc atgtacctca agtcgggttt catcgacgcg
     3901 ttcccggagg agatcgcgcg gacgctcgtc gagggctacg agcctcatcc ggagcgtggc
     3961 accaccgtgt tcttccagca cgcaggcggt gcgatcggcc gcgtgcctgc ggcggcgacc
     4021 gcctacccgc atcgccgcgc cacgcacaac atgttcgcgc tggtgtcatg gccgctcacg
     4081 gccgatgcga cgccgcacgt gaagtacatc aagtcgtact gggccacgct cgagcgccac
     4141 acgaacggct actacaccaa cgaggtcgcc gacgaaggcc agaacgtcat cgacgacaac
     4201 taccagggca acctgccgcg actgcggcag gtcaaggcca agtacgaccc gaacaacctg
     4261 ttccggctga acgccaacgt gaaaccgatg gcctgagtga gcgtcgcggc gggcgggacg
     4321 ggcccgctgc ggggctcact gcggcggcga ctcgcgccac gcccgccacc gttccaggcg
     4381 cgcctcacgc gtcgcaacga ggtggcgtga cacgcgcgcc tgcgcctctg ccgaccacgg
     4441 ggcggtcttc atcgccgctg catcgaccgg gctcgacgcg accgcgcccg tgatgaccgg
     4501 cacgcgatcg gcgtacacgt ccgtgcggtt cgccgcttcg ccgttgttgg tgcgcgaata
     4561 cacggccagg aacgtggacg cgttcgccgc gtcgaggccc tggtaatcgc cgacgaacag
     4621 cccgccagcg tccggcgcgt cgaacatgtc gaagttgccc gccagtcgcg tctcggccca
     4681 gtcgacgccg ttcgaggacg acgcgagcca gaagtcggtc ggctggtagg tcgcggtgcc
     4741 ggcctggcgg aagtcgtagt aggtcacgcc gatcgcaccg cccggcaaca cggccagcgt
     4801 gggcgtgaac gcgggcacgt cggcacgcgc gttgacgcgc gacggcgcgg tccacgtgag
     4861 gccgccgtcg ctcgattgcg agaccgcgat ggcatcgcgg gcgccgctgg aaaatcgcga
     4921 gtcctgccac gccgcgtaca acgtgccgtc gtccgggtcg gccgcgaagg tgccgaggat
     4981 ctcgccgctg cgcacgggtt gcccgccctg cggcgtcacg gtgccgaccg ggcgtgcctc
     5041 ggcgatgcgg gtcggcacgg accaggtggc acccttgtcg ctggaacgaa tcaccgagac
     5101 gtgggcttcg aggccggacg cgttcgccgg atccgggccg agttcgacga agaacacgtg
     5161 cacgatgccg tccggggtgg ttaccggcac gttaccgatc gtctgtcgac cggcaccggg
     5221 gtcgtagatc gtgcgcgtgg gctcccacga cgcgccgccg tcgatggagc gcgcgagcag
     5281 cgtcggcccg cgctcgtcga gcccgatgcg gtcccacacc gcgtacacgt agcgtggatc
     5341 ggtcgtgtcg atcgagagcg cttccttgtc gttgaacagg cggttgccgt cgtcgtcgac
     5401 gagcagcacg ccgggccccc acgtcacgcc gccgtcgacc gagcggctga cggcgacggc
     5461 gctgcgcgca ccgacgctgt tggcagcgcc cgtgaacgcg atgccgatct ggaggaccgt
     5521 gttgccgacc accgcgaccc acggatccga gactcgcgcg aaggcgcctc cggcgcacgt
     5581 ggagaacggt gccggctgcg gcgcgtccca ggtgaggcca ccgtcgatcg agacggccgt
     5641 ggccagaccg cgtgccccgc cgtccgacag gcgatcctgc tgccaggccg cgacgacgtg
     5701 gttcgggttg gtgcggtcga cggccaggtg aggctcgacc tcggcgttgg tgtagaccgt
     5761 cgagccggcg ggcacggcga ggcatccacc ggcgaacggc gtcgctcgac tcacgcgcac
     5821 ggccgtcgcg gtctggaata tcgccggttc gccgggtggc ggcgggttgc cgcccccacc
     5881 gccgctcgaa tcgcccccac cgccacaccc ggcgagcgag gcgacggcca gcacgacgag
     5941 tggccaggtg gcattcgcgg cgatgcgaat catcggacgg gacggcgatt gcaaagtcga
     6001 ggcgttggcc atgcgtgctt cccgtgccgc ggtatgaatc gtccgcggca gtataagcgc
     6061 accgcacccg gcgcctcgcc ggtccagcgg gcgcgagggt tgcattcgac ggccggtagc
     6121 ggcatatagg tcgcatggat ttcgccgctg catcgcaatt cctgaagtcg ccgagccggc
     6181 cgggccccgt cacggcctgg cttgcctgcg cccgacgccc gatctgaccc gcagcccctg
     6241 agcccgcgtc cgatcggacg gccgggctcg cttccagaac ccttcgtcat tcgtaccgca
     6301 agcgcgcggc cagcgcgcac tcgtcgcatt ttgcggctgc ttccagagga cctgaaccat
     6361 gaccacccta gcccgtgaca tctgcctgac tgaactcgac gccgtgcgcc tcgagagcat
     6421 gctcgaccag gtgcgccgcc gggcgcccga ggagcgttcg agcatcgaag cgctcgagga
     6481 gcgcgtggac gccgcccgcg tcgtgcacca gagctgcatc gatccgcagg tcgtgacgat
     6541 gaactcggtc gtggcgttga ccgaccgtgg gacgggcgag accgtcgagc tgacgctcgc
     6601 gtacgcagcc gaggccgacg ccgagcaagg tcgcgtgtcg gtgctgtccc cggtgggccg
     6661 cgcgctgatc ggcgcccgga tcggcgacgt gctgcgcgtc gccgtgccgg cgcagcgcga
     6721 caagaccttc gaggtgagcg cgatcccgta ccagcccgag gcgcagggac gctacgacct
     6781 ctgacgagcg gcgagccgcg cggccgggat caggccgcgc ggcgtcccag cagcgtgtcg
     6841 atcacgtcgt ccgcggagta accgtcgcgc ccgggacgca cgaagcgctg caggtcggtc
     6901 acatcgtcgc cggccgtcac cagcggcggt tcgagtccgc cgtcgcggcg ccgctgctcg
     6961 aaacccacgg tcgagacgag gccgttgcag acgcacttgc gtccggcggt ttccgcttcg
     7021 gtgccaccct tgcgcaggaa gtcctcgagt ggctcgcccg gacagcggta gccgacggtg
     7081 ccgtcctcgg tcttgtagca ctgccgcagg taggcgaggt cacagatgcg ctcgcgcgca
     7141 gcgtacaggt ccggctccga gacggtgccc ggcaggcgca cgaccttgaa cggaaagccg
     7201 gtcggcgagg cacggggatc ggtgaacacg cgcaggcagc cgtcgcggct cgcgcgcagc
     7261 accgacgacc tgagctcggg cgtc
//
LOCUS       DASPFR010000341         4231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1566406, whole genome shotgun sequence.
ACCESSION   DASPFR010000341 DASPFR010000000
VERSION     DASPFR010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566406"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="VD737_08940"
     CDS             <1..602
                     /locus_tag="VD737_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10728.1"
                     /translation="IAAHAQEQPAESPPPSPSQSHTTAPNTSTDAGGSAGGQAPVGTP
                     PSNVDEDSSSGSYYPYSSGTVGDDPAKPEATTVPVQAGTQGDQSSATTTNDWMTADTN
                     GDEQLSKDEIEQAWPTVGARFDEIDVDGDKQASRDELRNWHKSQKARMDADQPAGAPA
                     SAAPAATVPHTQAPADVPPVAPAPANDEPTTADPATTNQ"
     gene            730..2106
                     /locus_tag="VD737_08945"
     CDS             730..2106
                     /locus_tag="VD737_08945"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXW10729.1"
                     /translation="MYRLLVLCSVFVPLLAGAAAPEVDARRSAERELLRKVVEIPTVA
                     GRGEMPRLVKLLTAEFRKAGITDVTVKSHGDTQSMIVRWPAARPATRKPILLLAHMDV
                     VEARRADWVNDPFTFREADGFYWGRGTSDNKAGVVAIVASLARLRASGFEPGRDLVVL
                     FTGDEETRQDGARLASTEWRSLIDAEYALNSDAGGGGIYPDGRVEGFYVQVAEKTYAD
                     FRLAAVNRGGHSSSPRVDNAIYQLAGALKALEEYRFTPMLNDASRAYFEAVAANDQGL
                     WGATVRAWLDDPTNLEKADMVELNEPGYTRTRCVATQVTGGHAPNALPQRAEANVNCR
                     IFPGVKTEDVLEELRSIAGKEVAVELVDGGDWSDPSPLREDVMQAYRSAVVKRFPGAP
                     FVPSMSAGASDAIFTRRAGIPTYGVGGLWGYVGEPLGAHGLNERIRVDAFHDQVDIWE
                     AMLRELAR"
     gene            complement(2112..3428)
                     /locus_tag="VD737_08950"
     CDS             complement(2112..3428)
                     /locus_tag="VD737_08950"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HXW10730.1"
                     /translation="MPLPVARVSVQALYQSKLTSAVDAVRLVRDGEQIVVPTGAGEPP
                     ALLTALSDLRRDFRGVTVAQLLPLRPYGYLDAGTLDHVRHLSLFYGGPSRHGGQQGWI
                     DFVPNSFSDMPLLIERGYLAADVVFALASPMDDEGRFALSLATDYTLAAVARARCVVL
                     EVNPNVPYTYGNCHVHVSKVTAVVEDDAPVAEVALPKIGPVQEAIGRHVAELVDDGAT
                     LQIGYGSIPDAVVMQLTGKRDLGIHTEMLGDGILRLVEAGAVSNCRKNFMPGRMVATF
                     ALGSSRLYRYMDRRADLEMHPADFTNDPAVAGRNDDLVSINGTLQVDLLGQCGSESLG
                     SLPYSGTGGQSDFVRAANRSRGGKSFIVLPSTARDGSVSRIVATLAGGTHVSTSKNDV
                     NYVVTEYGVAQLRGRSARQRAQALIAIAHPDHRAELAGQARAQRLI"
     gene            complement(3471..>4231)
                     /gene="moeB"
                     /locus_tag="VD737_08955"
     CDS             complement(3471..>4231)
                     /gene="moeB"
                     /locus_tag="VD737_08955"
                     /inference="COORDINATES: protein motif:HMM:NF004281.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HXW10731.1"
                     /translation="TDQLQRYSRHFLLNQVGEKGQRKLLRSKALLIGAGGLGSPTALY
                     LAAAGVGHLTLVDDDRVERSNLHRQVLHGDADTGRLKVDSGAATLEALNPSVQVARVA
                     ERLTPDNARALLAHAHVVVDGADNFATRYAVNDACVALGLPDVHGSVFRFEGQVTVLW
                     PSAPGGGPCYRCLYPEPPPPELAPSCGEAGVLGVVPGVIGMLQATETVKLILGVGAPL
                     VGRMLQYDALRGSFDEFDVPRAADCPVCARRSDG"
BASE COUNT          649 a         1483 c         1519 g          580 t
ORIGIN      
        1 ccatagcggc gcatgcgcag gagcagccgg cggaatcccc cccgccttct ccgtctcaat
       61 cgcacacaac ggcaccgaac acgtccacgg acgcaggcgg ctcggccggt ggtcaggccc
      121 cggtcggcac gccgccgagc aacgtcgacg aggactcgag cagcgggtcg tactacccat
      181 actcctccgg cacggtcggt gacgatccgg cgaagcccga ggccaccacg gtaccggtcc
      241 aggcgggcac gcaaggggac cagtcgagcg cgaccacgac caacgactgg atgaccgccg
      301 acacgaacgg tgacgagcag ctttccaagg acgagatcga gcaggcgtgg ccgacggtcg
      361 gcgcacgttt tgacgagatc gacgtcgacg gcgacaagca ggcttcgcgc gacgagctgc
      421 gcaactggca caagtcgcag aaggcgcgca tggatgcgga ccagcccgcg ggcgctcccg
      481 cctcggcggc accagccgcc acggtcccgc atacgcaggc ccctgcggac gtgccgccgg
      541 tggcgccggc gccggcgaac gacgagccga cgacggcgga tcccgccacg acgaaccagt
      601 aacgcactgg ctcgagaccc ccggcgcccg tcgcgacgtt gcgacgggcg ctttcttttg
      661 tgcggtgggt cgggttgcgc agcgtgacac cgccccatac cctccgcgac tttgccccgg
      721 gggacatcga tgtatcgact gctggtgctc tgctccgtct tcgtgccgtt gctggccggc
      781 gcggctgcgc cagaggtcga tgcgcgtcgc agcgccgagc gcgaactgct ccgcaaggtc
      841 gtcgagatcc cgaccgtggc ggggcgcggc gagatgccac ggctcgtcaa gctgctgacc
      901 gccgagttcc gcaaggccgg catcaccgac gtgacggtga agtcgcacgg ggacacgcag
      961 tcgatgatcg tccgctggcc tgcggcgcgg cccgccacgc gcaagccgat cctgttgctg
     1021 gcgcacatgg acgtggtcga ggcgcggcgc gccgactggg tcaacgaccc tttcacgttc
     1081 cgcgaggccg acggcttcta ctggggccgc ggcaccagcg acaacaaggc cggcgtcgtg
     1141 gcgatcgtgg cgagcctggc ccggctgcgc gcatcgggct tcgagcctgg acgcgacctc
     1201 gtcgtgctgt tcaccggcga cgaggagacg cggcaggacg gcgcgcggct cgcgtccacg
     1261 gagtggcgat cgctgatcga cgccgagtac gcgctcaact ccgacgcggg cggcggcggc
     1321 atctatccgg acgggcgcgt ggaggggttc tacgtgcagg tggcggagaa gacctatgcc
     1381 gatttccgcc tggcggcggt gaaccgcggc ggtcacagct cgtcgccgcg cgtcgacaac
     1441 gcgatctacc agctcgcggg cgcactgaag gcgctcgagg agtaccgctt cacgccgatg
     1501 ctcaacgacg cgagtcgcgc gtatttcgag gcggtggcgg ccaacgacca gggcctgtgg
     1561 ggcgccaccg tccgcgcctg gctcgacgac ccgacgaacc tcgagaaggc ggacatggtc
     1621 gagttgaacg agccggggta cacgcgcacc cgctgcgtgg ccacccaggt caccggcggg
     1681 cacgcgccca acgctctgcc gcagcgcgcg gaagcgaacg tcaactgccg gatctttccc
     1741 ggcgtgaaga ccgaggacgt gctcgaggaa ttgcgctcga tcgcgggcaa ggaagtcgcc
     1801 gtggagctcg tcgacggcgg cgactggtcc gacccgagcc cgctgcgcga ggacgtgatg
     1861 caggcctacc gcagcgcggt cgtgaagcga ttcccgggcg caccgttcgt gccgagcatg
     1921 tcggccggcg cgtccgacgc gatcttcacg cgccgggccg gcatcccgac gtacggcgtc
     1981 ggcgggttgt ggggatacgt cggcgaaccg ctgggcgccc acggactgaa cgaacggatc
     2041 cgcgtcgacg ccttccacga ccaggtcgac atctgggagg ccatgctgcg cgagctcgcg
     2101 cgctgacggg atcagatgag gcgctgcgcg cgcgcctgtc cggccagctc cgcccggtgg
     2161 tccgggtgcg cgatcgcgat cagcgcctgc gcgcgctgcc gcgccgacct gccgcgcagc
     2221 tgcgcgacgc cgtactcggt cacgacgtag ttgacgtcgt tcttgctggt cgagacgtgc
     2281 gtgccgccgg cgagcgtcgc gacgatgcgc gagacgctgc cgtcgcgggc cgtcgacggc
     2341 agcacgatga acgacttccc gccgcgtgac cggttcgcgg cgcggacgaa gtccgactgg
     2401 ccgcccgtcc ccgaatatgg cagcgacccg aggctctccg aaccgcactg tccgagcagg
     2461 tcgacctgca gcgtaccgtt gatcgagacg aggtcgtcgt tgcgaccggc gacggccggg
     2521 tcgttggtga agtccgccgg gtgcatctcg aggtcggcgc gtcggtccat gtatcggtac
     2581 agccgcgacg accccagcgc gaacgtcgcg accatgcgtc cgggcatgaa gttcttgcgg
     2641 cagttgctga cggcaccggc ctccaccagc cgcaggatgc cgtcgcccag catttccgtg
     2701 tggatgccga ggtcgcgctt gccggtcagt tgcatgacga ccgcgtcggg aatgctgccg
     2761 tagccgatct gcagcgtcgc cccgtcgtcg acgagctcgg ccacgtgccg gccgatcgcc
     2821 tcctgcaccg ggccgatctt cggcagcgcg acttcggcga ccggcgcgtc gtcctcgacc
     2881 acggccgtca ctttcgagac gtgcacgtgg caattgccgt aggtgtacgg cacgttggga
     2941 ttgacttcga gcacgacgca gcgcgcgcgc gccaccgccg ccagcgtgta gtcggttgcg
     3001 aggctcagcg cgaaacgacc ctcgtcgtcc atcggcgagg cgagcgcgaa cacgacgtcg
     3061 gcggccaggt agccgcgctc gatcagcagc ggcatgtcgg agaagctgtt cggcacgaag
     3121 tcgatccagc cctgctgccc accgtggcgc gacggcccgc cgtagaacag cgacaggtgg
     3181 cggacgtggt cgagcgtgcc ggcgtcgagg taaccgtacg ggcgcagcgg cagcaactgc
     3241 gcgacggtga cgccgcgaaa gtcgcggcgc aggtccgaca gcgcggtcag cagcgcgggc
     3301 ggttccccgg cgccggtcgg cacgacgatc tgttcgccgt cgcgcacgag gcgcaccgcg
     3361 tcgacggcgg acgtgagctt gctctggtag agggcctgta cggacacgcg ggcgaccggc
     3421 agtggcaagc ccgctatgct gtcgacggcc ggccgtccgc gcagtacgtg ttagccgtcg
     3481 ctccgtcgcg cgcagaccgg gcagtcggcg gcgcgcggca cgtcgaactc gtcgaagctg
     3541 ccgcgcaagg cgtcgtactg cagcatccgg ccgacgagcg gcgcgcccac gcccaggatc
     3601 agcttgacgg tctcagtggc ctgcagcatg ccgatcacgc cgggcaccac gccgagcacg
     3661 cccgcctcgc cgcacgacgg cgcgagctcg ggcggcggcg gctccgggta gaggcatcgg
     3721 tagcacggcc ctccgccggg cgccgacggc cacagcacgg tgacctgccc ttcgaaccgg
     3781 aacaccgacc cgtgcacgtc cggcaggccg agcgccacgc aagcgtcgtt gaccgcatag
     3841 cgcgtcgcga agttgtccgc accgtcgacg acgacgtgcg cgtgcgcgag cagggcgcgc
     3901 gcgttgtccg gggtcaggcg ctccgccacg cgggccacct gcaccgacgg attcagggcc
     3961 tcgagcgtgg cggcgccgga gtcgaccttg agccggccgg tgtcggcgtc gccgtgaagc
     4021 acctgccggt gcaggttgct gcgctcgacg cgatcgtcgt cgacgagcgt caggtggccc
     4081 acgcccgcgg ccgcgaggta gagcgccgtc ggcgagccga gcccgcccgc gccgatcagc
     4141 agcgccttgc tgcgcagcag cttgcgctga cccttctcgc cgacctggtt gagcaggaaa
     4201 tgccggctgt agcgctgcag ctggtcggta t
//
LOCUS       DASPFR010000342         2227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1078727, whole genome shotgun sequence.
ACCESSION   DASPFR010000342 DASPFR010000000
VERSION     DASPFR010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078727"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VD737_08960"
     CDS             <1..547
                     /locus_tag="VD737_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10732.1"
                     /translation="QTETKTVVKVKRAGEISTITFPFNPLDAIGWKGDLMAVKLNWRD
                     IRPLMSHRFHLPPSAHTTFVAGRFVVCTFVPRPIESDPGALKVPFFHNNDDYDEVIFY
                     HAGDFFSRDNIHPGMVTFHPAGFTHGPHPKALARMFEQTKPATDEYAVMIDTRDSLDV
                     TDAGLAVEWDGYVDSWKGSRP"
     gene            544..1539
                     /locus_tag="VD737_08965"
     CDS             544..1539
                     /locus_tag="VD737_08965"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXW10733.1"
                     /translation="MKLATLRDGTRDGRLVVVSRDLARAVPAGTVAATLQDALDDWSR
                     VAPQLEQLYSRLQEGSVDHAEAFDPARAMAPLPRAHHWVDGSAYVNHVELVRKARNAE
                     MPPSFWTDPLVYQGGSDDLLGPMDDVPVASEEYGIDLEAEVAVVTDDVPMLTGEDAAR
                     GHIKLLMLVNDWSLRNLIPAELAKGFGFYQSKPATAFSPCAVTPDELGGAWSESRVQL
                     PLVSHVNGQLFGEPDAGVDMTFDFAQLIAHVTKTRRLGAGTIVGSGTVSNYDRSRGSS
                     CVAEKRALETVQHGKPLTTFLMFGDRVRIEMFGRDGASIFGAIDQKVVQARAGAT"
     gene            1591..>2227
                     /locus_tag="VD737_08970"
     CDS             1591..>2227
                     /locus_tag="VD737_08970"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXW10734.1"
                     /translation="MFDALDTLPPDPILGVTAAFRRDTYPLKVDLGVGVYRDEHGITP
                     VPAAVREAERALIASQSTKTYVGPAGNLEFNSRIVELALGPLASQLGARIATIQTVGG
                     CGALRVGAELVRAASPDAVIHVSTPTWANHEPLVGSSGLRLERYPYYDPVTRDVDFDA
                     MHDHVSRLPAGSVVLLHGCCHNPTGADLRLDQWRALADVIERRGLLPFVDMA"
BASE COUNT          353 a          778 c          750 g          346 t
ORIGIN      
        1 gcagaccgag accaagacgg tggtcaaggt gaagcgcgcc ggcgagatca gcaccatcac
       61 gtttccgttc aatccgctgg acgccatcgg gtggaagggg gatttgatgg cggtcaaact
      121 caattggcgc gacatccgcc ccctgatgag ccaccgcttt cacttgccgc cctcggcgca
      181 caccacgttt gtggccgggc ggttcgtggt gtgcacgttc gtgccccggc ccatcgagtc
      241 cgaccccggc gcgctcaagg tgccgttttt ccacaacaac gacgactacg acgaagtgat
      301 cttctatcac gcgggcgatt tcttcagccg cgacaacatc cacccgggca tggtcacgtt
      361 ccacccggca ggcttcactc acggcccgca cccgaaagcg ctcgcgcgca tgttcgagca
      421 gaccaagccg gcgacggacg aatacgcggt catgatcgac acgcgcgatt cgctcgacgt
      481 cacggacgcc gggctcgccg tcgaatggga cggctacgtc gacagctgga agggctcgcg
      541 gccgtgaagc tcgcgacgct gcgggacggc acccgcgacg gccggctcgt ggtcgtgagc
      601 cgcgacctcg cgcgcgcggt gcccgccggc accgtcgcgg cgacgctcca ggacgcgctc
      661 gacgactggt cgcgcgtcgc gccgcaactc gagcagctct actcccgctt gcaggaaggt
      721 tcggtcgacc acgccgaggc cttcgatccg gcgcgggcga tggcgccgtt gccgcgtgcc
      781 caccattggg tggacggctc ggcctacgtg aaccacgtgg agctcgtgcg caaggcgcgc
      841 aacgccgaga tgccgccgag cttctggacc gatccgctcg tctaccaggg cggctcggac
      901 gacctgctcg ggccgatgga cgacgtgccg gtcgcgagcg aggagtacgg catcgacctg
      961 gaggcggaag tggcagtcgt caccgacgac gtcccgatgc tgaccggcga ggacgcggcg
     1021 cgcgggcaca tcaagctgct gatgctcgtc aacgactggt cgctgcgcaa cctgattccc
     1081 gccgaactgg cgaagggctt cggcttctac cagtcgaagc ccgcgacggc gttctcgccg
     1141 tgcgcggtga cgccggacga actcggcggc gcctggtcgg agagccgcgt gcagctgccg
     1201 ctggtctcgc acgtcaatgg acagctcttc ggcgagcctg acgcgggcgt cgacatgacg
     1261 ttcgatttcg cgcaattgat cgcgcacgta acgaagaccc ggcggctggg cgccggcacg
     1321 atcgtgggtt ccggcacggt gtccaattac gaccgctcga gggggtcgag ctgcgtggcg
     1381 gaaaagcggg ccctcgaaac cgtgcagcac ggcaagccgc tcacgacgtt cctcatgttc
     1441 ggcgatcgcg tgcgcatcga gatgttcggt cgcgacggtg ccagcatctt cggagcgatc
     1501 gaccagaagg tcgtgcaggc gcgcgccggc gcgacctgaa caccagcgcg tttcggtggc
     1561 atgatgcgcc gatccagcac aggcgatgcc atgttcgacg cgctcgatac actcccgccc
     1621 gatccgatcc tcggcgtcac ggcagccttc cgtcgcgaca cctatccgct caaggtcgac
     1681 ctcggcgtcg gcgtgtatcg cgacgagcac ggcatcacgc ccgtgccggc ggccgtccgc
     1741 gaggccgagc gtgccctgat cgcgtcgcag tcgaccaaga cgtacgtcgg gcccgccggc
     1801 aacctggagt tcaactcgcg catcgtcgag ctggcactcg ggccgcttgc gtcgcagctc
     1861 ggggcgcgca tcgcgaccat ccagaccgtc gggggctgcg gcgccctgcg ggtcggcgcc
     1921 gaactcgtcc gtgccgccag cccggacgcg gtcattcacg tcagcacgcc gacgtgggcc
     1981 aatcacgagc cgctggtcgg cagttccggg ctgcgcctcg aacgctatcc gtactacgac
     2041 ccggtcaccc gcgacgtgga cttcgacgcg atgcacgacc acgtctcacg cctcccggcc
     2101 gggtcggtcg tgctgctgca cggctgctgc cacaacccga cgggggccga cctccgcctg
     2161 gaccagtggc gggcgctcgc ggacgtgatc gagcggcgtg ggctgctgcc gttcgtcgac
     2221 atggcct
//
LOCUS       DASPFR010000343        15902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_955812, whole genome shotgun sequence.
ACCESSION   DASPFR010000343 DASPFR010000000
VERSION     DASPFR010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15902
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955812"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VD737_08975"
     CDS             <1..959
                     /locus_tag="VD737_08975"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HXW10735.1"
                     /translation="AYNVKRITTTDYTPEQIHQLGLDEVARIHRAMDGVMQQVGFEGT
                     RNEFFKHLNTDPRFFWTSREDLVAGYEKIKAEVDPLLPKLFEILPKADYEVRAVEPFR
                     EKSAAGGQYQAASEDGSRPGIFYANAYDLRARPKWAMEALSLHEGNPGHHFQITIQRE
                     QKDLPRFRRFGGYTAYSEGWGLYAESEPVGRALGRYTDPYQYFGQLEAEIWRAIRLVV
                     DTGLHSKGWTREQVLDYMEANSSAAEARRVSETERYMAIPGQALAYKIGQIKISELRA
                     RAEAALGPKFDVRKFHTAVLVDGALPLDVLESKIDRWIESQR"
     gene            971..2365
                     /locus_tag="VD737_08980"
     CDS             971..2365
                     /locus_tag="VD737_08980"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXW10736.1"
                     /translation="MKRIRSRAGRAVLLASCLATGTAAAATTPKSATLPGADQIVTRT
                     LGNGLKVVVWPDHDIPNVAMYTWYRVGSRNERPGITGISHFFEHMMFNGTKTRPPGEF
                     DRVMEANGGRNNAYTNSDVTVYQNWFPSTTTSLIFDLESDRMRNLDFDPKKVESERGV
                     VYSERRSGVDDDNFGTLVEQMQATAFVAHPYQIPTIGWPSDIEGWKVDDLRAYYQQYY
                     APNNAVMFVVGDVDPAAVFRLADQYVGAIPAQPAPPPVTTVEPPQLGERRITIEREAQ
                     TPLLAMAWHAAAAPDRETRVMEVLLSILGDGESSRLYRRLVDQEQAAVDVGTALDQGF
                     DPGLAWVYAVVPPGGDVAKTEQLVDEEIARLTRDGPTPEELAKARNQALAGFWRGLET
                     ISGKAQALGTYEVFHGDYRKLFEAPAAYEGITADDVRKAASAMLRPGNRTVGHLVPPS
                     TATGDDAVPAGGAR"
     gene            2374..3786
                     /locus_tag="VD737_08985"
     CDS             2374..3786
                     /locus_tag="VD737_08985"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXW10737.1"
                     /translation="MFPRHAQPLLAALLLVGTAPSFAAAPGVELPPVQRARLPNGAEL
                     ALVEKRDTPLVAMTVTVRGGALGDPTDREGTASLFADLIQKGAGTRNAAQFAEAVESA
                     GGMIVAAAGPESLSINASFLARDVDLMLQLVGDALQRPTLAASEFEKSRTLAIQSIAA
                     AKDSDPRALISSYGDAWLFRGHPYGRPVGGSEASLAAVTLDDVRRYYQDQVGGDRTII
                     TVVGDIDASAMRTKLESALGGWRKAAATLPSAPAPQEVRGRRVLLVDKPGATQTYFWL
                     GDVGASRTDPARTQQSVVNTAFGGRYTSMLNSALRIDSGLTYGASATFDRGLQPGAYA
                     IASYTQTGSTVQAIDLALSTLGRLRRDGLDADTLASAKSYMLGQFPPTLETNGQIAAR
                     LADLAFYGLGIEDVEGYAQRVAAVDAAAVRQAIQAFPSGDDLAIVLIGDAAQIRGQVA
                     KYGPITEMKITDPRFAPPPK"
     gene            3783..4424
                     /locus_tag="VD737_08990"
     CDS             3783..4424
                     /locus_tag="VD737_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HXW10738.1"
                     /translation="MSGRRLTALLAGASGLVGGEVLRRLLDESDYASVVLVTRRDLGA
                     SAKHRKLRQVVGDFARLEQRLDDLRADHVFCALGTTIAKAGSQAKFREVDFEYPRRLA
                     EVTLGTGASHFSLVSALGADRRSPFFYSRVKGELEDAVRAMTWPSLCIVRPSLIAGNR
                     AESRPLERLAEHALRFAPRTWRPVHARKIAAAMVATALLSPPGVTVIESRDMQ"
     gene            complement(4428..4604)
                     /locus_tag="VD737_08995"
     CDS             complement(4428..4604)
                     /locus_tag="VD737_08995"
                     /inference="COORDINATES: protein motif:HMM:NF021639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10739.1"
                     /translation="MIGLVNLAVSFSLALRVALRSRGIGPEQTHGLWGRVLRRFFQSP
                     RDFFWPRPVAAQTP"
     gene            complement(4688..4930)
                     /locus_tag="VD737_09000"
     CDS             complement(4688..4930)
                     /locus_tag="VD737_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10740.1"
                     /translation="MDYLHSSSSGPARVHTLAVDRTRAEALMLVLEAGFDSLIARTAA
                     ASKENPDAAERRQVLEKLETAEAVLEELEDAFVTAH"
     gene            complement(5013..5972)
                     /gene="tal"
                     /locus_tag="VD737_09005"
     CDS             complement(5013..5972)
                     /gene="tal"
                     /locus_tag="VD737_09005"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HXW10741.1"
                     /translation="MTNQLESLRRHSVIVADSGDIEAIASYRPQDATTNPSLIFKAAQ
                     EPRYARLVDAAVAAAPGRGHDEARLTTIIDRLMVGFGQEILKLIPGRVSTEVEARLSF
                     DTAASERKARHLVELYEEAGIKRDRVLIKLASTWEGIRAAERLERDGIRCNMTLLFSF
                     AQAVACADAGVTLISPFVGRIYDWYRKSRGVDDIAREDDPGVASVTRIYEYYKKHGYA
                     TQVMGASFRKTTQILDLAGCDLLTISPDLLARLEATAGEVPARLTVDVARRSDVEKVA
                     LDEPAFRWLHNEDAMATEKLAEGIRAFDADARKLAAWLQHRIG"
     gene            complement(5990..8458)
                     /locus_tag="VD737_09010"
     CDS             complement(5990..8458)
                     /locus_tag="VD737_09010"
                     /inference="COORDINATES: protein motif:HMM:NF003619.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HXW10742.1"
                     /translation="MAKKHGKRALAPVQARNPAPARRSAGPAVTRERTRADATLDRTE
                     LQRIDAYWRACNYLAAGMIYLRDNPLLLEPLTPDHIKNRLLGHWGASPGLSFTYVHLN
                     RLIRKYDLDAIFLAGPGHGAPGVLAPVYLEGTYAEIYPNKGEDVEGMRQFFKEFSFPG
                     GIGSHCTPETPGSIHEGGELGYSISHAFGAAFDNPDLMVTVVVGDGEAETGPLATAWH
                     SNKFLNPVRDGAVLPVLHLNGYKINNPTLLARITHEELDALFRGYGWTPHFVEGDDPA
                     QMHELMATTLERCVLEIRRIQDEARRSGKAERARWPMIVLRSPKGWTGPKKVGGKTVE
                     GYWRAHQVPLADVRSNPENLKQLEKWLMSYRPRELFDANGRLDEDLKALAPTGRRRMS
                     ANPHANGGQLRRPLRLPDFQDYAVEVKKPGASEAENTRRLGEFLRDILKLNPDNFRVF
                     GPDETTSNKLDAVYEASKKTWLAGYLPVDADGGQLAPDGRVMEMLSEHTLEGWLEGYL
                     LTGRHGFLSTYEAFVHVIDSMFNQHAKWLAISRELPWRSPVASLNMLITSTVWRQDHN
                     GFTHQDPGFLDIVVNKSPEVTRIYLPPDSNCLLAVSDHCLRSTDAINVIVCDKQKHLQ
                     YLDMDAAVTHCEKGIGLWDWASSDAGSVPDVVMVGCGDIATQEALAATALLRHHFPDL
                     KLRFVNVVDLYTLTPPKEHPHGLSDRDFDSLFTTDRPIIFNFHGYPWLIHRLAYRRRN
                     HDNLHVRGYKEKGNINTPLELAIDNEVDRFSLAIDVIDRVPRLQVAGAHVKEWLRNQQ
                     IECRKYAHEHGIDRPSDRDWRWPL"
     gene            8593..10089
                     /locus_tag="VD737_09015"
     CDS             8593..10089
                     /locus_tag="VD737_09015"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HXW10743.1"
                     /translation="MRIAVGGFQHETNTFSEHLATLAHFEAPDAWPGLTRGPALFEAV
                     AGINLPAAGFVAEARGLRHDLVPLTWCSAQPSGRVTRDAFEHVTALLEEDLRLSGGVD
                     AVYLDLHGAMVAEHVDDADGEVLRRIRSLVGPDVPVVASLDYHANVSMQMVEAASALV
                     AYKTYPHVDMAECGARAARCLHDLLGKPRPARAYSTVDFLVPLTSQSTLASPMRELAA
                     MAVELERPPLLAVNVAPGFPAADVAECGPSVYACGWDAERAQEAVERLAAAFREGETE
                     FALELYTVEQACRLAQSATVPRGRPLVLADTQDNPGAGGNADTMALIKALSRAHVPRV
                     LAGVICDPVAAACAHAAGTGARVDLELGGRSGPAGESPFAARFEVLALGDGRFVGTGP
                     FYRGGRFELGPMALLRLGELHLAVASRKQQAADQAMFRHLGVDPSQFSVLALKSSVHF
                     RADFGPIASRIVVVEAPGSNVADPSKLPFTRLRPGMRTSPRRQDSGAR"
     gene            10132..10560
                     /locus_tag="VD737_09020"
     CDS             10132..10560
                     /locus_tag="VD737_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXW10744.1"
                     /translation="MRKILVAIDGSEAASRALNYAVTQARCEPAAVLHVLNVQPTLSN
                     YTAPEIYVTAERIREVTAARARAILDAAAAHLKDVGCRYELEQVEGEPAETIARRASE
                     LGCESITMGTHGLTSIGILFMGSVAQKVVYHSDVPVTLVK"
     gene            complement(10567..11901)
                     /locus_tag="VD737_09025"
     CDS             complement(10567..11901)
                     /locus_tag="VD737_09025"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HXW10745.1"
                     /translation="MSYEAIALLMFGTFMVLLLTGQRVFGAIGFVAAVFALSLWGEGA
                     VEMPFASTITLLNWYPMLTLPMFVYMGYMLSESGIASDLYRMFHVWTGPMRGGLAVGT
                     ILLMIAISAMNGLSVAGMAIGASIAMPELLKRGYDKIMVTGVIQAGSTLGIMVPPSVV
                     LVLYGMIARQPVGQLWLAGVGPGLLLGALFIIYITIRCQLQPHLGPPLPAEERAQITW
                     GEKLRLLRAGVLPLLIIFSMTGLFVMGATSMVESSAVGALSATLVAAYKRRITWQVLK
                     DTLRKSLAVSAMFMWIILAALAFGAVFDGLGAVRAIEVLFLEKWGLEPWQVLVLMQLT
                     YIVMGTFLDDTAMLIIVAPLYIPLVIALGFNPIWYGILYTITCQIAYVTPPFGYNLFL
                     MRAMAPKEVTLVDIYRSIVPFVLIMILGLALVVAFPQIALWLPEHVYLKSTP"
     gene            complement(11898..12410)
                     /locus_tag="VD737_09030"
     CDS             complement(11898..12410)
                     /locus_tag="VD737_09030"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HXW10746.1"
                     /translation="MPKPIKLYVRYVDAINRVLGNVVMFLALVMMGVLLFGSLTRYVF
                     NVPFVWIIEMAQFLMAAYYILGGGYSMQLDAHVRMDVLYERWRPRTRAFMDSITSFFL
                     IFYLVVMMQGGIASSLYSLKYNQTNYSAWAPPMAPIKIVMTVGIALMLLQAVSILFKD
                     IAKFTGREIA"
     gene            complement(12528..13619)
                     /locus_tag="VD737_09035"
     CDS             complement(12528..13619)
                     /locus_tag="VD737_09035"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HXW10747.1"
                     /translation="MQRRTFLTQAALAATAASAAALTACGGKDGESAGDGAPAVQTSK
                     KTAIKWRLQTYAGPALAEHVIKPSIDAFNKAANGEMVIELYFADQLVPTGELFRAMQA
                     GTIDAVQSDDDSIAAPTDIRVFGGYFPFATRYSLDVPALFHKYGLNEIWKEAYDEVEG
                     VTWLSSGSWDPCHFNTVKPINKLSDLKGLRIFTFPTAGKFLARFGVVPVTLPWGDVQV
                     AIQTGELDGLAWSGITEDYTVGWADVTPYFLTNNISGAWIGSYFVNDKSWEKVPEHLK
                     TLYRLCIDSSHYYRQHWYWAGEADLRVNGPKMKLTTIPAAEWATVEDEAHKFWDEIAA
                     TSPRCAKVVKIFRDYNAQMSKAGPPYRYA"
     gene            13895..15259
                     /locus_tag="VD737_09040"
     CDS             13895..15259
                     /locus_tag="VD737_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HXW10748.1"
                     /translation="MEAREVRTAADARVLVEERGVQHVKVGVFDVDGILRGKYISREK
                     FLSALDSGFGFCDVVLGWDCQDQLYDNARFTGWHTGYPDAPARILPETCRTLPFEDGG
                     LFFLGEFAPPAETVCPRALLRRVVGRAEALGFTAYAGFEYEFFVFRETPESIRAKHYR
                     DPTPMAPGWFGYSVLRNSAGSDFYRVLLDQCREMDMAIEGLHEETGPGVLEAAIAFDQ
                     ALASADKAALFKTFAKVIAQKHGLLATFMAKWSPDVPGQSGHIHLSLKKDGKPVFHDP
                     GQPHTMSDTMRWFVGGQQKLMPELLAMIAPTVNSYRRLIPGFWAPTDATWGVENRTTA
                     LRVIPGSPKSQRVEYRVAAADANPYVILAAALGSGLWGVEQRIEPEPVVTGNAYDRKY
                     PEHLALPRTLWDAAQRLRGSQVARELFGDDFVEHFAATREWEEREFRKHISDWELARY
                     FEII"
     gene            15431..>15902
                     /locus_tag="VD737_09045"
     CDS             15431..>15902
                     /locus_tag="VD737_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXW10749.1"
                     /translation="MPLQRTISPIDNSVCVERELASGRAIDDALDRARRAQREWRRVP
                     VATRAEILGRFCDAFERRREAVAQELAWQMGRPVRYGPNEVRGTLERARHMIAIAPEA
                     LADVDVGPKEGFVRFLRREPLGVVFTVAAWNYPYLIAVNSVVPALMAGNVVVLKH"
BASE COUNT         2498 a         5415 c         5477 g         2512 t
ORIGIN      
        1 acgcctacaa cgtcaagcgc atcaccacga ccgactacac gcccgagcag atccaccagc
       61 tcggtctcga cgaggtcgcc cggatccacc gggcgatgga cggcgtgatg cagcaggtgg
      121 gcttcgaggg cacccgcaac gagttcttca agcacctcaa caccgacccg cggttcttct
      181 ggacctcccg cgaggacctc gtggcgggct acgagaagat caaggccgag gtggatccgc
      241 tgctgccgaa gctgttcgag atccttccga aagcggacta cgaggtgcgc gccgtcgaac
      301 ccttccgcga gaagagcgcc gcgggcggcc agtaccaggc ggcgagcgag gacggctcgc
      361 gcccgggcat cttttatgcc aacgcctatg acctgcgcgc gcggccgaag tgggccatgg
      421 aggcgctctc gctgcacgag ggcaaccccg gccaccactt ccagatcacg atccagcgcg
      481 agcagaagga cctgccgaga ttccgtcgct tcggcggcta taccgcgtac tccgaaggct
      541 ggggtctcta cgcggagtcc gagccggtcg gccgcgcgct cggccgctac accgacccgt
      601 accagtattt cggccagctc gaggccgaga tctggcgcgc gatccggctc gtcgtagaca
      661 ccgggttgca ctcgaagggc tggacgcgcg agcaggtgct cgactacatg gaagccaaca
      721 gctcggcggc cgaggcgcgt cgcgtctccg agaccgagcg ctacatggcg atcccgggac
      781 aggcgctcgc gtacaagatc ggccagatca agatcagcga gctgcgcgca cgtgccgagg
      841 ccgcgctggg cccgaagttc gacgtgcgca agttccacac ggccgtgctc gtcgacggcg
      901 cgctgccgct cgacgtgctc gaatcgaaga tcgaccgctg gatcgaaagc cagcgctgag
      961 gacacccgcg atgaagcgta tccgctcgcg tgccggccgc gccgtactgc tggcctcctg
     1021 cctggccacc ggcaccgccg cggccgcgac gacgccgaag tccgccacgc tgccgggcgc
     1081 cgaccagatc gtcacccgca ccctgggcaa cggcctgaag gtcgtggtgt ggccggacca
     1141 cgacatcccg aacgtcgcga tgtacacgtg gtaccgcgtc ggcagccgca acgagcgacc
     1201 gggcatcacg ggcatctcgc acttcttcga gcacatgatg ttcaacggca cgaagacgcg
     1261 gccgcccggc gagttcgacc gcgtgatgga ggcgaacggc ggccgcaaca acgcctacac
     1321 gaactcggac gtgacggtct accagaactg gttcccaagc acgacgacgt cgctgatctt
     1381 cgacctcgag tccgaccgga tgcgcaacct cgatttcgat ccgaagaagg tcgagagcga
     1441 gcgcggtgtc gtttattcgg agcgtcggtc gggcgtggac gacgacaact tcggcacgct
     1501 cgtggaacag atgcaggcga cggcgttcgt cgcgcacccg taccagatcc cgaccatcgg
     1561 ctggccctcc gacatcgaag gctggaaggt cgacgacctg cgcgcgtact accagcagta
     1621 ctacgcgccg aacaacgccg tgatgttcgt cgtgggcgac gtcgacccgg ccgcggtctt
     1681 ccggctcgcc gaccagtacg tgggggcgat tccggcgcag ccggcccccc cgccggtgac
     1741 cacggtcgag ccgccgcagc tgggcgagcg gcgcatcacg atcgagcgcg aggcgcagac
     1801 gccgctgctc gcgatggcct ggcacgcggc cgccgcgccg gatcgcgaga cgcgcgtgat
     1861 ggaagtgctg ctctcgatcc tgggcgacgg cgagtcgtcg cgcctctacc ggcggctggt
     1921 cgaccaggag caggccgcgg tcgacgtcgg cacggcgttg gaccagggct tcgacccggg
     1981 tctcgcgtgg gtgtacgcgg tggtgccgcc gggcggcgac gtggcaaaga ccgagcaact
     2041 cgtcgacgag gaaatcgcgc gcctcacccg ggacgggccg acgcccgaag aactggcaaa
     2101 ggcccgcaac caggctttgg cgggcttctg gcgcggcctc gaaaccatca gtggcaaggc
     2161 gcaggcgctc ggcacctacg aagtcttcca cggcgattac cgcaagctct tcgaggcgcc
     2221 cgcagcctac gagggcatca cggcggacga cgtccggaag gcggcgtccg cgatgctgcg
     2281 tcccggcaac cggaccgtcg gccacctggt gccgccttcc acggcaacag gtgacgacgc
     2341 ggtccccgcc ggaggtgccc ggtgatcgcc gcgatgttcc cgagacacgc acagcccctg
     2401 ctcgcggcgc tgctgctggt gggcaccgcg ccgtcgttcg cggcggctcc cggcgtcgag
     2461 ctgccaccgg tccagcgcgc acggctgccg aacggcgcgg agctcgcgct cgtggagaag
     2521 cgcgacacgc cgctggtcgc catgacggtc acggtgcgtg gcggcgcgct tggcgacccg
     2581 acggaccggg aaggcaccgc gtcgctgttc gccgacctga tccagaaggg cgccggaacg
     2641 cgcaacgcgg cgcagttcgc cgaggccgtc gagagcgcgg gcggcatgat cgtggcggcc
     2701 gccggtccgg agagtctgtc gatcaacgcc agcttcctgg cgcgggacgt ggacctgatg
     2761 ctgcagctcg tcggtgacgc gctccaacgg ccgacgcttg cggcgtccga gttcgagaag
     2821 tcccggacgc tcgcgataca gtcgatcgct gcggccaagg actcggatcc gcgcgcgctg
     2881 atctcgagct acggcgatgc ctggctgttc cgcggtcatc cctacgggcg cccggtcggt
     2941 ggcagtgaag cctcgctcgc ggccgtgacg ctcgacgacg tgcgtcgcta ctaccaggac
     3001 caggtcggtg gcgaccgcac gatcatcacg gtcgtgggcg acatcgacgc gagcgccatg
     3061 cgcacgaagc tggagagcgc ccttggcggc tggcgcaagg cggcagcgac gctcccgtcc
     3121 gcgccggcgc cgcaggaggt ccgtggccgg cgcgtgctgc tcgtcgacaa gccgggcgcc
     3181 acgcagacgt atttctggct cggcgacgtc ggcgcctcgc gcaccgaccc ggcacgcacg
     3241 cagcagtcgg tcgtcaacac cgcgttcggc ggacggtaca cctcgatgct gaattcggcg
     3301 ctgcgcatcg acagcggact cacctacggc gcgagtgcca ccttcgaccg cggcctgcag
     3361 ccgggcgcct atgcgatcgc ctcgtacacg cagaccggct ccacggtgca ggcgatcgat
     3421 ctcgcgctct cgacgctcgg gcggctgcgc cgcgacgggc tcgacgcgga cacgctcgct
     3481 tccgcgaaga gctacatgct cggccagttc ccgccgacgc tcgagaccaa cggccagatc
     3541 gcggcccggc tcgccgacct cgcgttctac gggctcggca tcgaagacgt cgaggggtat
     3601 gcgcagcgcg tcgcggccgt cgacgcggcg gcggtgcggc aggcgatcca ggcgttcccg
     3661 agcggcgacg acctggcgat cgtgctgatc ggcgatgctg cgcagatccg cgggcaggtc
     3721 gcgaagtacg ggcccatcac cgagatgaag atcaccgacc cgcggttcgc gccgcccccg
     3781 aagtgagcgg tcgacgcctg accgcgctgc tcgccggcgc ctcggggttg gtcggcggcg
     3841 aggtcctgcg acgactgctg gacgaaagcg actacgcgtc ggtggtgctg gtgacccggc
     3901 gcgacctggg tgcctcggcg aagcaccgca agctgcgaca agtcgtaggt gatttcgccc
     3961 gcctcgagca acgcctggac gacctgcgag ccgatcacgt gttctgcgcg ctcggcacga
     4021 ccattgccaa ggccggatcg caggcgaaat tccgcgaggt cgatttcgag tatccgcgcc
     4081 ggctcgccga agtcaccctg ggaacgggcg cgagccattt ctcgctcgtc agcgcgctgg
     4141 gcgccgaccg acgctcgccg ttcttctatt cgcgcgtcaa gggcgaactc gaagacgcgg
     4201 tgcgcgcgat gacgtggccg agcctgtgca tcgtgcggcc gtcgctgatc gcgggcaacc
     4261 gggccgagtc gcggccgctc gagcggctgg cggaacacgc actgcggttc gcgccccgga
     4321 cctggcgccc cgtccatgcg cggaagattg ccgcggcgat ggttgccacg gccctgctgt
     4381 cgccacctgg cgtgaccgtg atcgagtcgc gggacatgca atgagcatca cggtgtctgc
     4441 gccgcgaccg ggcgcggcca gaagaagtcg cggggcgact ggaagaaccg gcgcaggacc
     4501 cggccccaga gtccgtgggt ctgttccggg ccgatgccgc gcgaacgcaa cgcaacccgc
     4561 agcgccagcg aaaaactcac ggcgaggttg accaggccga tcatcagccg cggagtatcg
     4621 ccgaggatcg ggccgcgttg aagcttccgg cgcgaggcac ccgggcggca ccgaccgccc
     4681 aggtggctca gtgcgcggtg acgaaagcgt cctcgagttc ctcgagcacg gcctccgcgg
     4741 tctcgagctt ctcgaggacc tgcctgcgct cggcggcgtc cgggttttcc ttcgaggccg
     4801 ccgccgtccg ggcgatcagc gaatcgaagc cagcttcgag caccagcatc agcgcctcgg
     4861 cccgtgtgcg gtcgacggcg agcgtgtgga cccgcgccgg acccgacgat gacgagtgca
     4921 ggtagtccat gttgcctcct cgagttcccc gggtgacggc ctcgatcctc aaccgacatc
     4981 tcgtcgcgcg cgaggttccg cgcaaagggc cctcaaccaa tccggtgctg cagccaggcc
     5041 gcgagcttgc gggcatcggc atcgaacgca cggatgcctt cggcgagctt ctcggtcgcc
     5101 atcgcgtcct cgttgtgcag ccagcggaac gccggctcgt cgagcgcgac cttctcgacg
     5161 tcgctgcgac gcgccacgtc caccgtcagt cgcgcgggca cctcgcccgc cgtcgcctcg
     5221 agcctggcga gcaggtccgg agagatcgtc agcaggtcgc agcccgcgag gtccaggatc
     5281 tgtgtcgtct tgcggaagct cgcgcccatg acctgcgtcg cgtagccgtg cttcttgtag
     5341 tactcgtaga tgcgcgtgac cgaggcgacg cccgggtcgt cctcgcgcgc gatgtcgtcg
     5401 acgccgcgcg acttcctgta ccagtcgtag atgcggccga cgaacggcga gatcagcgtg
     5461 acgcccgcgt ccgcgcacgc gacggcctgc gcgaagctga acagcagcgt catgttgcag
     5521 cggatgccgt cgcgctcgag gcgctcggcc gcgcggatgc cttcccaggt cgaggccagc
     5581 ttgatcaaca cccgatcacg cttgatgccg gcctcttcgt acagctcgac caggtggcgc
     5641 gccttgcgct cggacgccgc cgtgtcgaag ctcagtcgtg cctcgacctc ggtcgacacc
     5701 cggccgggaa tcagcttcag gatctcctgc ccgaaaccga ccatcagccg gtcgatgatg
     5761 gtggtgagcc gcgcctcgtc gtggccgcga cccggcgcgg ccgcgaccgc tgcgtcgacc
     5821 agccgggcgt agcgtggctc ctgcgcggcc ttgaaaatca ggctgggatt ggtggtggcg
     5881 tcctgcgggc gatagctcgc gatcgcctcg atgtcgccgg agtcggcgac gatcaccgag
     5941 tgacggcgca gcgattcgag ctggttggtc acggtgttgg gttcggcact cacaggggcc
     6001 acctccagtc gcggtcggac ggtcgatcga tgccgtgctc gtgcgcgtat ttgcggcact
     6061 cgatctgctg gttgcgcaac cactccttga cgtgcgcgcc ggccacctgc agccgcggta
     6121 cgcggtcgat cacgtcgatc gcgaggctga agcggtccac ctcgttgtcg atcgcgagct
     6181 cgagcggcgt gttgatgttg cctttttcct tgtagccgcg cacgtgcagg ttgtcgtggt
     6241 tgcggcggcg gtaggccagc cggtggatca gccacggata cccgtggaag ttgaagatga
     6301 tcggccggtc ggtcgtgaac agcgagtcga agtcgcggtc cgacagcccg tgcggatgct
     6361 ccttgggcgg cgtcagcgtg tagaggtcga cgacgttcac gaagcgcagc ttcaggtccg
     6421 ggaagtggtg acgcagcagc gccgtggcgg ccagggcttc ctgcgtcgcg atgtcgccgc
     6481 agccgaccat cacgacgtcg ggcaccgaac cggcgtcgct gctcgcccag tcccagagcc
     6541 cgatgccctt ttcgcagtgc gtgaccgccg cgtccatgtc caggtactgc aggtgcttct
     6601 gcttgtcgca gacgatcacg ttgatcgcgt cggtgctgcg caggcagtgg tccgagaccg
     6661 ccagcaggca gttcgaatcg ggtggcaggt agatccgggt gacctccggg ctcttgttga
     6721 cgacgatgtc gaggaagccc gggtcctggt gcgtgaagcc gttgtggtcc tggcgccaga
     6781 cggtcgacgt gatcagcatg ttgagcgacg cgaccggcga tcgccacggc agctcgcgcg
     6841 agatcgcgag ccacttggcg tgctggttga acatcgagtc gatcacgtgc acgaacgctt
     6901 cgtaggtcga gaggaagccg tgccggcccg tgagcaggta gccttccagc cagccttcga
     6961 gcgtgtgctc cgacagcatc tccatgaccc gcccgtcggg ggcgagctgg ccgccgtccg
     7021 cgtccaccgg caggtaaccg gcgagccacg tcttcttgct ggcctcgtag acggcgtcca
     7081 gcttgttgga cgtggtctcg tcgggcccga acacgcggaa gttgtccggg ttgagcttca
     7141 ggatgtcgcg caggaactcg ccgagccggc gcgtgttctc ggcttcggaa gccccgggct
     7201 tcttgacctc gacggcgtaa tcctggaaat cgggcagtcg cagcggacgt cgcagctggc
     7261 cgccgttggc gtgcgggttc gcgctcatgc gtcgccggcc cgtgggggcg agtgccttca
     7321 ggtcctcgtc gaggcgcccg ttcgcgtcga acagctcgcg cgggcgatag ctcatcagcc
     7381 acttttcgag ctgcttgaga ttctctggat tcgagcgcac gtcggcgagt ggcacctggt
     7441 gcgctcgcca gtacccttct accgtcttgc cgcccacctt cttgggaccg gtccagccct
     7501 tcgggctgcg cagcacgatc atcggccagc gcgcgcgctc ggccttgccg gagcgtcgcg
     7561 cctcgtcctg gatgcggcgg atctcgagca cgcagcgctc gagcgtggtc gccatcagct
     7621 cgtgcatctg cgccgggtcg tcgccctcga cgaagtgcgg cgtccagccg tagccgcgga
     7681 acagcgcgtc gagctcctcg tgcgtgatcc gcgcgagcag cgtcgggttg ttgatcttgt
     7741 agccgttcag gtgcagcacc ggcagcaccg cgccatcgcg cacggggttc aggaacttgt
     7801 tcgagtgcca ggcggttgcg agcgggcccg tctcggcttc gccgtcgccc acgacgacgg
     7861 tgaccatcag gtcgggattg tcgaacgccg cgccgaacgc gtgcgagatg ctgtagccga
     7921 gctcgccgcc ctcgtggatc gagccgggcg tctcgggcgt gcagtggctg ccgataccgc
     7981 cggggaacga gaactccttg aagaactgcc gcatgccctc gacgtcttcg cccttgttcg
     8041 ggtagatctc ggcgtacgtc ccctcgaggt agaccggcgc cagcacgccc ggcgccccgt
     8101 ggccgggtcc ggccaggaag attgcgtcca ggtcgtattt ccggatcagc cggttgaggt
     8161 gcacgtaggt gaagctcagg cccgggctcg ccccccagtg gccgagcagc cggttcttga
     8221 tgtggtccgg cgtcaggggc tcgagcagca gtgggttgtc gcgcaggtaa atcatgccgg
     8281 cggccaggta gttgcaggcc cgccagtacg cgtcgatgcg ctgcagttcg gtcctgtcga
     8341 gcgtcgcgtc ggcgcgcgtc cgctcgcgcg tcaccgccgg ccccgccgac ctccgtgcgg
     8401 gagccgggtt gcgcgcctgc accggcgcga gcgcgcgctt gccgtgtttc ttcgccatca
     8461 cgtccctctc cgtgggccag cgatcgtagc cccttacttt cgcgcaggcg tccggccgac
     8521 gccagtccaa atacgcaggc agtcgcaaat ccgtcgtgac gtccgacacg aggcgcgcgc
     8581 taacatgtcc tcatgcgcat tgccgtcggc ggcttccagc acgaaacaaa cacgttctcg
     8641 gaacacctcg ccacgctcgc gcacttcgaa gcgcccgacg catggccggg cctcacgcgc
     8701 ggtccggcgc tgttcgaggc cgtcgcgggc atcaacctgc ccgcggcagg gttcgtggcg
     8761 gaggcgcgcg ggctgcgcca cgacctcgtg ccgctcacct ggtgctccgc gcagccgtcc
     8821 ggtcgagtca cgcgcgacgc tttcgagcac gtcaccgcgc tgctcgagga agaccttcgc
     8881 ctgtctggcg gggtcgatgc cgtctacctc gacctgcatg gggcaatggt ggccgagcac
     8941 gtcgacgacg cggacggcga ggtgctgcgt cgcatccgct cactggtcgg cccggacgtt
     9001 cccgtcgtcg cgagcctcga ctatcacgcc aacgtctcga tgcagatggt cgaggccgcc
     9061 tcggcgctcg tggcgtacaa gacctatccg cacgtcgaca tggccgagtg cggcgcgcgc
     9121 gctgcccggt gcctgcacga cctgcttggc aagccgcgtc cggcccgcgc ttactcgacc
     9181 gtggacttcc tggtgccgct gacctcgcag tccacgctcg cctcgccgat gcgcgaactc
     9241 gcggcgatgg cggtggaact cgagcgaccg ccgttgctgg ccgtcaacgt cgcaccgggg
     9301 ttcccggcgg cggacgtggc cgaatgcggt ccgagcgtct acgcgtgtgg ctgggacgcg
     9361 gagcgcgcgc aggaggccgt cgagcggctg gccgcggcct tccgcgaggg cgagaccgag
     9421 ttcgcgctcg agctctacac ggtcgagcag gcgtgccggc tcgcgcaatc cgcgaccgtt
     9481 ccgcgcggac gacccctggt tctcgcggac acccaggaca atccgggcgc gggcggcaat
     9541 gccgacacga tggcgctgat caaggcgctg tcacgcgcgc acgtcccgcg cgtgctggcc
     9601 ggcgtgatct gcgatcccgt ggcggccgcg tgcgcccacg cggcgggcac cggcgcacgg
     9661 gtcgacctcg agctcggcgg ccggtccggc ccggcgggag agtcgccgtt cgcggcgcgg
     9721 ttcgaagtgc tcgcgctcgg cgacggccgc ttcgtgggga ccgggccgtt ctaccgcggc
     9781 ggccggttcg aactcggccc gatggcgctg ctgcggctgg gcgaactgca cctcgcggtc
     9841 gcgagccgca agcaacaggc ggcggaccag gcgatgttcc ggcacctggg cgtcgatccg
     9901 agccagttct cggtgctggc gctcaagagc tccgtgcatt ttcgggccga cttcggcccg
     9961 atcgcgagcc gcatcgtcgt ggtggaggcg ccgggatcga acgtcgccga tccgtcgaaa
    10021 ctgccgttca cgcggctgcg gccggggatg cgaacgagtc cccgccgcca ggactccggc
    10081 gcacggtgag cacagacggt cgctgtacac tgcccgcaag gagggctcgc gatgcgcaag
    10141 atcctggtgg cgatcgacgg ttcggaggcg gcctcgaggg cgctcaacta cgccgtgacg
    10201 caggcgcgct gcgagcccgc ggcggtgctg cacgtgctga acgtgcagcc cacgctcagc
    10261 aactacacgg cgccggagat ctacgtgacc gcggagcgca tccgcgaggt gaccgcggcg
    10321 cgcgcacggg cgatcctcga tgcggctgcg gcgcacctca aggacgtcgg ttgccggtac
    10381 gagctcgagc aggtcgaggg cgagccggcc gagacgatcg cgcgccgtgc cagcgaactc
    10441 ggctgcgaat cgatcacgat gggcacgcac gggctcacgt cgatcggcat cctgttcatg
    10501 gggtcggttg cgcagaaggt cgtctaccac tccgacgtgc ccgtcacgct cgtgaagtga
    10561 cccgcctcag ggcgtgctct tcaagtagac gtgctccggc agccagagcg cgatctgcgg
    10621 gaacgcgacc acgagcgcca gccccaggat catgatcagc acgaacggca cgatcgaccg
    10681 gtagatgtcg accagcgtga cttccttggg cgccatcgcg cgcatcagga acaggttgta
    10741 cccgaagggt ggcgtcacgt acgcgatctg gcaggtgatg gtgtagagga tgccgtacca
    10801 gatcgggttg aagccgagcg cgatcaccag cgggatgtag agcggcgcga cgatgatcag
    10861 catcgccgtg tcgtccagga acgtgcccat cacgatgtag gtgagctgca tcagcaccag
    10921 cacctgccac ggctcgaggc cccacttctc gaggaacagc acttcgatcg cgcggaccgc
    10981 gccgagcccg tcgaagaccg cgccgaacgc gagcgccgcc aggatgatcc acatgaacat
    11041 cgcgctcacg gcgaggcttt tccggagcgt gtccttcagg acctgccagg tgatgcggcg
    11101 cttgtaggcc gccacgagcg tggcggagag ggcgccgacg gccgacgact cgaccatgct
    11161 cgtcgcgccc atgacgaaca ggccggtcat cgagaagatg atcagcagcg gcagcacgcc
    11221 ggcccgcagc aaccgcagct tctcgcccca ggtgatctgc gcgcgctcct cggccggaag
    11281 cggcgggccg aggtgcggtt gcagctggca gcggatcgtg atgtagatga tgaacagcgc
    11341 ccccagcagc aggcccggtc cgacgccggc cagccacagc tgtcccaccg gctggcgcgc
    11401 gatcatcccg tagagcacga gtaccacgct cggcgggacc atgatgccga gcgtgctgcc
    11461 ggcctggatc acgccggtga ccatgatctt gtcgtagccg cgtttcagca gctcgggcat
    11521 cgcgatcgac gcgccgatcg ccatgccagc cacgctgagg ccgttcatcg ccgagatggc
    11581 gatcatcagc aggatcgtcc cgaccgcgag tccgccgcgc atcggcccgg tccagacgtg
    11641 gaacatcctg tacaggtcgc tcgcgatgcc ggactccgac agcatgtaac ccatgtagac
    11701 gaacatgggc agcgtcagca tcgggtacca gttgagcagc gtgatcgtcg aggcgaacgg
    11761 catctcgacc gcgccctcgc cccacagcga gagcgcgaag accgccgcca cgaacccgat
    11821 cgcgccgaac acgcgctgcc ccgtgagcag cagcaccatg aaggtgccga acatcagcag
    11881 ggcgatcgcc tcgtagctca tgcgatctcc cggcccgtga acttcgcgat gtccttgaac
    11941 aggatcgaga ccgcctgcag cagcatcagc gcgatcccga ccgtcatgac gatcttgatg
    12001 ggggccatcg gcggtgccca ggcggagtag ttggtctggt tgtacttgag cgagtacagg
    12061 ctgctcgcga tcccgccctg catcatcacg accaggtaga agatcaggaa gaacgaggtg
    12121 atcgagtcca tgaaggcccg ggtccgcggc cgccagcgct cgtagagcac gtccatgcgc
    12181 acgtgcgcgt cgagctgcat cgaatagccg ccgccgagga tgtagtaggc ggccatcagg
    12241 aactgtgcca tctcgatgat ccagacgaac gggacgttga agacgtaccg agtcagtgac
    12301 ccgaacagca gcacgcccat catcacgagg gcgaggaaca tcacgacgtt gccgagcacg
    12361 cggttgatcg cgtcgacgta tcgcacgtag agcttgatgg gcttgggcat gacggcgcct
    12421 cgggtggtca gcggcgatgc gcttcagggg ctgcgcacag gcgtccccgg cctgcgaggg
    12481 ccggggacgt cgccgacggg gtcggccgtg agcgagagat ctcctcgtca ggcgtagcgg
    12541 tagggcgggc ccgccttcga catctgcgcg ttgtagtccc tgaagatctt gacgaccttg
    12601 gcgcagcgcg ggctcgtcgc cgcgatctcg tcccagaact tgtgggcctc gtcctcgacc
    12661 gtcgcccatt ccgccgccgg gatcgtcgtc agcttcatct tcgggccgtt gacgcgcagg
    12721 tcggcctcgc cggcccagta ccagtgctgg cggtagtagt gcgagctgtc gatgcagagc
    12781 ctgtagaggg tcttcaggtg ctccggcacc ttctcccagg acttgtcgtt gacgaagtac
    12841 gagccgatcc aggcacccga gatgttgttg gtcaggaaat acggcgtgac gtccgcccag
    12901 cccacggtgt agtcctcggt gatgcccgac cacgcgaggc cgtcgagttc gccggtctgg
    12961 attgcgacct gcacgtcgcc ccatggcaac gtgaccggca ccacgccgaa acgcgccagg
    13021 aacttgcccg cagtcgggaa cgtgaagatg cgcaggccct tgaggtccga gagcttgttg
    13081 atcggcttga cggtgttgaa gtggcagggg tcccaggaac ccgagctgag ccaggtcacc
    13141 ccttcgacct cgtcgtaggc ttccttccag atctcgttca ggccgtactt gtggaacaaa
    13201 gccggcacgt cgaggctgta gcgcgtcgcg aacgggaagt agccgccgaa cacgcggatg
    13261 tcagtcggcg ccgcgatcga gtcgtcgtcg ctctgcaccg cgtcgatcgt gcccgcctgc
    13321 atggcgcgga acagctcgcc ggtcgggacg agctggtccg cgaagtagag ctcgatgacc
    13381 atctcgccgt tggcggcctt gttgaacgcg tcgatcgacg gcttgatgac gtgctccgcc
    13441 agcgccgggc cggcgtaggt ctgcaggcgc cacttgatcg ccgtcttctt cgacgtctgg
    13501 actgccggtg caccgtcacc tgcggattcg ccgtctttgc cgccgcaggc cgtcagagcc
    13561 gcagccgaag ccgccgtggc cgccagtgcg gcctgtgtca ggaacgttct acgctgcatc
    13621 aacctctcct tttttcgaat cgttgacgac accgaagtcc ctggcgcccg cagtgcattg
    13681 tcgttgaggg cgagcggggc ctgcgtgcca cgagtgctcg acgaaccgac tcatagcggt
    13741 aagcttccac caaagtcaag ccggcgtgcg gccgcgcgcc gtgcggtcgt gggccgcgcg
    13801 gcggggcggc gtgtcccgcg gccgtgccac gtcgagcggc aattcggcta aagtcgtgcg
    13861 gcccgacgcg agcatccggg cacggggagg cgacatggaa gcgagggagg tgcgcacggc
    13921 ggcggacgcc cgcgtgctgg tcgaggaacg cggcgtgcag cacgtcaagg tcggcgtgtt
    13981 cgacgtcgac ggcatcctcc gcggcaagta catctcgcgc gagaagttcc tgtcggcgct
    14041 cgacagcggc ttcggcttct gcgacgtggt gctgggctgg gactgccagg accagctcta
    14101 cgacaacgcc cgtttcacgg gctggcacac cgggtacccg gatgcgccgg cgcgcatcct
    14161 tccagagacc tgtcgcacgc tgcctttcga ggacggcgga ctgttcttcc tcggtgaatt
    14221 cgccccgccg gccgagaccg tgtgtccgcg cgcgctgctg cgccgagtcg tcggccgcgc
    14281 cgaggcgctg ggattcaccg cgtacgccgg tttcgagtac gagttcttcg tcttcaggga
    14341 aacgcccgag tcgatccgcg cgaagcacta tcgcgacccg accccgatgg cgccgggctg
    14401 gttcggctac tccgtgctgc gcaacagcgc cggcagcgat ttctaccgag tgctgctcga
    14461 ccagtgccgc gagatggaca tggcgatcga gggcctgcac gaggagaccg gccccggcgt
    14521 actggaggcc gcgatcgcat tcgaccaggc gctggcctcg gccgacaagg ccgcgctgtt
    14581 caagaccttc gccaaggtca tcgcgcagaa gcacggcctg ctggcgacct tcatggccaa
    14641 gtggtcaccg gacgtgcccg ggcagagcgg ccacatccac ctgtcgctca agaaggacgg
    14701 caaaccggtg ttccacgacc ctggccagcc gcacacgatg agcgacacga tgcgctggtt
    14761 cgtcggcggc cagcagaaac tgatgcccga gctgctcgcg atgatcgcgc cgaccgtcaa
    14821 ttcctatcgc cgcctgattc ccggcttctg ggccccgacc gacgcgacgt ggggcgtgga
    14881 gaaccgcacc accgcgctgc gcgtgatccc gggcagcccc aagtcgcagc gcgtcgagta
    14941 ccgcgtggcc gccgccgacg cgaacccgta cgtgatcctg gcggccgcgc tcggctcggg
    15001 cctgtggggc gtcgagcagc gcatcgagcc cgagcccgtg gtcaccggca atgcctacga
    15061 ccgcaagtac cccgagcacc tggcactgcc acgcaccctg tgggacgcgg cccagcgcct
    15121 gcgtggctcg caagtggcgc gtgagttgtt cggcgacgac ttcgtcgagc attttgcggc
    15181 cacccgcgag tgggaggagc gcgagttccg taagcacatc agcgattggg aactcgcgcg
    15241 ctatttcgag atcatttgac gaagcggcta gtggtcagtg gctagtggct agtggctagt
    15301 gcggaaggcg ccgcgccgga gcctcacccg gcctgcggcc ggcctctcct gccaagccgg
    15361 gagaggtgtt ccggatcgtt cactagccac tagccactga tcactagcca cttcttccta
    15421 cggagctccc ttgcctctac agcgaaccat ctctcccatc gacaactcgg tctgcgtcga
    15481 gcgcgagctc gcgtccggcc gggcgatcga cgacgcgctc gaccgggccc gccgcgcgca
    15541 gcgcgagtgg cgtcgcgtgc cggtcgcgac acgcgcggaa atcctcggcc gcttctgcga
    15601 cgcgttcgag cggcggcgcg aggcggtcgc gcaggaactc gcgtggcaga tgggtcgtcc
    15661 cgtgcgctac gggccgaacg aggtgcgggg aacgctcgag cgcgcgcgac acatgatcgc
    15721 gatcgcgccg gaagcgctcg cggacgtcga cgtgggcccc aaggaagggt tcgtccgatt
    15781 cctgcggcgc gagccgctcg gcgtcgtgtt cacggtggcg gcctggaact acccgtacct
    15841 gatcgccgtc aacagcgtcg tgccggcact gatggccggc aacgtcgtcg tgctcaagca
    15901 ct
//
LOCUS       DASPFR010000344        15662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1567103, whole genome shotgun sequence.
ACCESSION   DASPFR010000344 DASPFR010000000
VERSION     DASPFR010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15662
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567103"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(547..2295)
                     /locus_tag="VD737_09050"
     CDS             complement(547..2295)
                     /locus_tag="VD737_09050"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HXW10750.1"
                     /translation="MSRTAASLLREALEHAGVRYTFGIPGVHNTEIYDELARSATITP
                     VLVTHEGGGAFMADAVSRSGTSLGTLVIVPAAGVTHAASGIAEAFLDGIPMLVISGGI
                     RRDTGRQYQLHDVNQHALLKPITKGTWLVERHADVIPTIHEAVRVATSGEPGPVFVEI
                     PVNLQLLTGDPGPSPMPRHQGPAIPEARCTAAGIGAAADLLANARSPSIFVGWGAVDA
                     SAEVAALAERLGAPVSTTLQGVSAFPATHPLHAGFCLGRAAVPAVEKTFEHTDCMLAV
                     GTRFGEIATGSYGWEPPANLIHVDVNPAVFNANYPAKVCLEGDARLVLQKLTAALDIA
                     APASAASRRQAVEATIREQKAAYLEQWRRHDSRGRVNPARFFTSLRRQLADDATVVLD
                     DGNHTFLAAELLPVHRPRGMISPTDFNCMGYCVPATIGAKLARPGSQVVGIVGDGAFR
                     MTAMELATATALDLGVVIFVFSDGELAQISQAQQVPYNRKTCTVLGRLDVAGVAQATG
                     ASFLRLANDADVDTVVSSALARAREGKPVLVDVAIDYSKRTRFTEGIVKTNLERLDLS
                     TKVRLVGRALWRRLSG"
     gene            2277..3473
                     /locus_tag="VD737_09055"
     CDS             2277..3473
                     /locus_tag="VD737_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXW10751.1"
                     /translation="MRRSGSRRPSFRSDCKSSAAILGTAHSSSHPLDPHSLGHPSCPA
                     RADRAQNGLPRQTSQGGCARVDEVRIAVIGAGVAGLACAQELLRADARVTVFERSRGL
                     GGRLATRRQGDLAFDHGAQFITARSRAFVGYAQAAVRADVLDAWRPRLMEDDRSWPAP
                     IEDWWIGKPGMSALVRPLARNLEIHTGVAVHELLPSQRGWELQTDSGRQHQVFRAVAV
                     AIPAPQAATLLGPHGRAFRHIADVRMAPCWTGMFAFDRPLDLGAEARRWTAGPLVWAA
                     CNSSKSQRLRRPQCWVVHASSSWSRERLELDAPEAARQLFETFAHAVGRALPAPSFST
                     AHRWRHALVEQPLGLQSVVDEEISAGACGDWCIAPRVEAAYESGRSLAHSILSMVGLS
                     ARMSRA"
     gene            complement(3422..4438)
                     /locus_tag="VD737_09060"
     CDS             complement(3422..4438)
                     /locus_tag="VD737_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10752.1"
                     /translation="MPDPRRGRWKKPLGVAVSTVLAGLAAWVLWRTFQRISFADVLAN
                     MRAVPASTLALAAICVAGAFTTLAFYEVAVVRYVKHCIGRAKPMVTAFIAFPLGHAVG
                     QAMLSGGALRYRMYTPAGFAATEVGATVLLANLPYGLAFGLLLDLALVMESTTLEPLF
                     RIQAPWLFALGCAGLAKDVGYALTIWLRRKPVKLGGWAVHLPTPAMTALQVVVGLVDI
                     GLVSTVLYLLLPEGTGLGYLPFLAVYLASVLAGVLSHVPAGLGVLESMLLLLLPSVPP
                     EQLLAAVLMYRVIYEIVPALCALVLWAAFEGFSRDGVLSRLLPRRGQARDIRADSPTM
                     DRIE"
     gene            4648..4983
                     /locus_tag="VD737_09065"
     CDS             4648..4983
                     /locus_tag="VD737_09065"
                     /inference="COORDINATES: protein motif:HMM:NF013517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HXW10753.1"
                     /translation="MSDQKFPRRTLLKGALLGVAAVPVSALLSRTADAAAGKVDPNEP
                     QAKSLGYVEDATKVDPKANPNYKPGQVCANCLQAPAAKSGGAEVPCNIFAGRNVAAKG
                     WCKVYVKRP"
     gene            5192..5653
                     /locus_tag="VD737_09070"
     CDS             5192..5653
                     /locus_tag="VD737_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXW10754.1"
                     /translation="MRVIKWEPFRDMDDVFNRFFADTLRRFPMQSGVGAGHEGAQQPR
                     EWAPLADVSENDGEYVIKAEVPEVRKEDVSVTVQDGVLTLSGERKQEQREENERVHRI
                     ERFHGTFARRFALPENADEQGIRAECRDGVIVIRIPKQKQVEQQPRQIQIQ"
     gene            5786..6283
                     /gene="rraA"
                     /locus_tag="VD737_09075"
     CDS             5786..6283
                     /gene="rraA"
                     /locus_tag="VD737_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HXW10755.1"
                     /translation="MTTSKSLAKGTADLCDEHGERVQVCEPIFQAYGGRKSFHGAVST
                     VRCFEDNSRVKEAVEGPGEGRVLIVDGGGSRRRALLGDKLGEAAVRNGWAGVIVYGCI
                     RDSAELGRIDLGIRALGTMPLRSDKRGEGERDVPVRFAGVTFRPGEFVYVDEDGVITA
                     QEALT"
     gene            6389..6661
                     /locus_tag="VD737_09080"
     CDS             6389..6661
                     /locus_tag="VD737_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011347875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HXW10756.1"
                     /translation="MRTVFCQYLQREAPGLERVPYPGELGRRIYDNISAEAWQAWVRH
                     QTMLINEYRLSPIEPKARKFLVEEMEKFLFAGGAERPAEYVEPDQE"
     gene            complement(6727..7146)
                     /locus_tag="VD737_09085"
     CDS             complement(6727..7146)
                     /locus_tag="VD737_09085"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HXW10757.1"
                     /translation="MTDEPRRNVVDRRFAMRCIGAGALLMLIAVMLGAFGAHALQAQL
                     TPRRLASFQTGVTYQQLHALALALLGLVACVTPRTRWLTVAAALFGAGIVLFCGSIYA
                     MTFGAPRALGMVAPLGGVSFMVGWACLALHARRVVSG"
     gene            complement(7143..8216)
                     /gene="mutY"
                     /locus_tag="VD737_09090"
     CDS             complement(7143..8216)
                     /gene="mutY"
                     /locus_tag="VD737_09090"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF008132.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HXW10758.1"
                     /translation="MTDVEFAPALLRWYEQDGRKHLPWQRDPTAYRVWVSEIMLQQTQ
                     VATVAGYYERFMARFPDVRRLAAAPLDEVLHLWSGLGYYARARNLHRAAQAIVQRHGG
                     EFPATLDEVMELPGIGRSTAGAILALSRGERHPILDGNVKRVLARYFGVEGYPGEPAV
                     EQRLWALAEACTPADGVDAYTQAIMDLGATICTRANPACLLCPVNAGCLALAGERQHA
                     LPAPRPRKPRPHREGWLVVARRGREVLLERRPPSGLWGGLWAFPEFPTRAHALQWCSE
                     NLSSPAAPHAGNPLHHAFSHFDYEMKPIFVDCLGVQARAMREGDRCLWYDTGAPAELG
                     LPKPVATLLQRAMDPRPDAEDAA"
     gene            complement(8213..10024)
                     /locus_tag="VD737_09095"
     CDS             complement(8213..10024)
                     /locus_tag="VD737_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10759.1"
                     /translation="MAELKRRRVGKVAIAYGAVAWGVTESASVVLPALHVPEWSVTFI
                     VVFLLVGFPIAMVLAWIFDATPTGIQRTAPLPGDAPQSRVRMRAAFGVLVLLAMAGLG
                     YLLYERGFARAQAGEPHSSIAVLPFTNLSGDPAKDYFSDGMSEELLNLLARIPGLQVA
                     ARTSAFAYKGRNVDIREVGRELGVETVLEGSVRQAGDKVRITAQLIDTETGFHLWSET
                     YDRELKDIFVVQDEIAQAIVERLRIQLAPDEQQFAQRDKAPTQDVEAYELYLQGQAIW
                     KRRGEENLRRAVELYQQAIGRDPGFAAAHAALASAYVVLPGYSKEEDDEEKYLKMAEE
                     SARQALAIDPKIGEAHAVLAQINSDRGNLLDAESGFFFAISLDPNEPTPHHWYSILLN
                     SVGRLDAGLEQARRAFELDPSSPILASNLANAYLLRGDDQQAARYAKLAEDLGLTNKG
                     SGVAAMIAMRQGRWEDAKTLFQHGDTPEKLKPLVAQWIDAVADPAKRPKVVAAMQEID
                     PSIVEQDDLLMPYLQLGQVDIVYDIMFDALDRNRTAWAHKWDVMNAWSIESAAFRRDP
                     RFPELARRMGLLDYWKQYGFPDACHAGSGDTAVVCVS"
     gene            complement(10186..11085)
                     /locus_tag="VD737_09100"
     CDS             complement(10186..11085)
                     /locus_tag="VD737_09100"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXW10760.1"
                     /translation="MPRRTLPAWSCALALLTAWPGIAAAGTPINKRTTADPHGTVEVS
                     NVAGSVTVTGWNRNEVEVTGELGDGTETLEFTKSEKLTRVKVVVPKRSYNVDDSELIV
                     KVPAGSVLSVNTVSADIVVKGVRGAQRLQAVSGDVRTEASGEDVECRTVSGDVTISGS
                     GQKGLVSITTVSGDATVTSVAGEVNGNTVSGSFDFGAGEISRSRLRSTSGDLTLTGQL
                     GPDARLDAESISGDVRLDFAGAVAGQFDLSTFNGEIRNCFGPKAQRTDEYAPGRELRF
                     QEGSSAARVRVKTLNGDIGVCRD"
     gene            complement(11103..11738)
                     /locus_tag="VD737_09105"
     CDS             complement(11103..11738)
                     /locus_tag="VD737_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10761.1"
                     /translation="MTDTERRDGSDDPRLDAQLRGLSYQGIEPGHDLWPTVAARLEPR
                     VAGSPSRRRAWAWQAAAAVLLVAGSSLLTATLLDTRGEDGTMATATIPAAGTVPTGAA
                     AVPAAFGAGARLDPDYLEARRQLTALLDTRMANLPESTRAKLEVNLAEMRRAALQINA
                     ALAEQPGDPLLEELLLKTYQDELAVLANVNQLAGALAGTADPKADPKRMQL"
     gene            complement(11725..12324)
                     /locus_tag="VD737_09110"
     CDS             complement(11725..12324)
                     /locus_tag="VD737_09110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXW10762.1"
                     /translation="MPKGEEVVDELQRARQAAEERRLVAAAVEGSVPAFEALYRGHVA
                     RIHALCLRMTGNVATAEDCTQEAFVQAWKHLPRFETRSAFGTWLHRIAVNAVLQQARR
                     RRESLGMEDSVEREVADVMVDHAASDPASGRDLEAAIASLPRGARHVLVLVGVYGHSH
                     EEAAGMLGIAVGTCKAQLHRARALLGARLAARETDHDGH"
     gene            12453..13244
                     /locus_tag="VD737_09115"
     CDS             12453..13244
                     /locus_tag="VD737_09115"
                     /inference="COORDINATES: protein motif:HMM:NF006108.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HXW10763.1"
                     /translation="MTDAVRVDRAGPVATVVLERPAVRNAVDPTTAAALAAAFASLDD
                     DPAIHSIVLWGAGGTFCAGADLQAVAQGWDPARLRAPDGSPRDGYGPMGPTRMELGKP
                     VIAAVAGHAVAGGLELALWCDLRIAEADALFGVYCRRWGVPLIDGGTVRLPRLVGAGR
                     AMDMILTGRAVGAEEALAIGLVDRVVPNGRSREAAEQLAAEIARFPQGCLRADRASAL
                     AQWSLPVTAALAREYESGLAVLRSGESARGAARFVSGAGRGGRPA"
     gene            13276..13707
                     /locus_tag="VD737_09120"
     CDS             13276..13707
                     /locus_tag="VD737_09120"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HXW10764.1"
                     /translation="MPIQSPIFAPATALVLWSLVMLGWLALTRIPAMTKAGIPVMKIV
                     GSRGVNLEGVVPDPVNWKSHNYAHLMEQPTLFYATVVILGVVGAGDGLNLQLAWAYVV
                     LRVVHSIVQATWNRVAVRFTLFALATAVLAVLAINAARLVF"
     gene            complement(13704..15002)
                     /locus_tag="VD737_09125"
     CDS             complement(13704..15002)
                     /locus_tag="VD737_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXW10765.1"
                     /translation="MLKLLRLAPFALLACGPAGAASDDAALDAAMKSVMPQVTTWRRD
                     FHQNPELSNREVRTSKVVAEQLRTLGFEVRTGIAHTGVVGILRGAKPGPTIALRADMD
                     ALPVTEQVDVPFKSVATAEYRGEKVGVMHACGHDAHTAILLGVARILGDMRNALPGTV
                     VFVFQPAEEGAPDGERGGASLMLDEGLFAAAKPEAMFGLHVFSTLNVGQVGYRTGPLM
                     AASDRFRIVVRGRQTHGARPWGGVDPIVTAAQVVTGLQTIVARQVDIAQYPAVVSVGA
                     IKGGIRNNIIPDSVEMVGTFRTFDPAVREQVIERIRRIATDTATAAGATAEFELGPDP
                     NPVVVNDPSLTARAVTTLERVTGKDQVAVMPYLTVSEDFAYYGQQVPSFFYFVGSTPQ
                     GQDPATAPNNHSPRFFLDEAALPIGVRTLTALALDYLEGR"
     gene            15080..>15662
                     /locus_tag="VD737_09130"
     CDS             15080..>15662
                     /locus_tag="VD737_09130"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXW10766.1"
                     /translation="MSQPTKSVAVVAALSLFCMTTAPSSSNAQARAAPTPPAGQAVSP
                     SVPRSISKTQKPPLHGQRWMAITGKPLGATAGAMMFQRGGNAVDAACAMIAATSTMWD
                     VLSWGGETQALIYDPRAKKVVAINGLGVAPSGATAEFYRGKGYKYPPEHGPLAAVTPG
                     TPGGVMLMLQEYGRLSLAEVLQPAIELADGYPID"
BASE COUNT         2355 a         5523 c         5415 g         2369 t
ORIGIN      
        1 gccgcgttcc agcgtcgcca ggtgcttcat caccgcgtgg cggccgatct cgtcggcaaa
       61 gaactcgcag acccgattga cgttgcagcc gccttccttc atcagcaggt cgagcatgcg
      121 gcggcgcagc tccgagctga gcgcgaagaa cgtcgcgtcg aggtcgtcgt ccgtcatatg
      181 tgaccagatg gtcacatgtc ggacgaccgc ggtcaagcgc ctctctttaa cccctccagc
      241 gcgagcggaa gaggtcgcac cacgtgcggg cgagggcctg gtcgctggaa gtgtccgtgc
      301 tgcggccgtc gatccctccg cgacatcttc cgcggcggga cgccgtgaac ccgtcccggg
      361 gggctccggc cgtgacatcc atgtcgcgga cggcccgccg cgaaagatct cgcgagggct
      421 cgccggccgc gttgacgtcg gtgcacgcaa tcaagccgct tgcatcagac gctgctcgcc
      481 taacgacctg ccgtctcccc gggtcgaaat ccggggacag cgccgccgcg tgctatagca
      541 gcttcctcag ccgctgaggc ggcgccagag cgcgcgaccg acgagccgca ccttcgtgga
      601 gagatccaac cgctcgaggt tggtcttgac gatgccttcg gtgaagcgcg tgcgtttcga
      661 gtagtcgatc gcgacgtcga cgagcaccgg cttgccctcg cgcgcgcgtg ccagggcgct
      721 gctcaccacc gtgtcgacgt ccgcatcgtt cgcgagccgc aggaacgacg cgcccgtcgc
      781 ctgtgccact cccgcgacgt cgagccggcc gagcaccgtg cacgtcttgc ggttgtacgg
      841 cacctgttgc gcctgcgaga tctgcgcgag ttcgccgtcg ctgaacacga agatcaccac
      901 gccgaggtcg agcgcggtcg ccgtggcgag ctccatcgcc gtcatccgga acgcgccgtc
      961 gccgacgatg ccgacgacct gcgatcccgg gcgcgcgagc ttggcgccga tcgtggccgg
     1021 cacgcagtag cccatgcagt tgaagtcggt cggcgagatc atgccgcgcg gccggtgaac
     1081 cggcagcagt tccgcggcga ggaacgtgtg gttgccgtcg tcgagcacga ccgtggcgtc
     1141 gtccgcgagc tgtcgcctca ggctcgtgaa gaaccgcgcc gggttcacgc gcccgcgcga
     1201 atcgtgtcga cgccactgct cgaggtaggc ggccttctgc tcgcggatgg tcgcctcgac
     1261 ggcctggcgg cgggacgcag cgctcgccgg cgcggcgatg tccagcgccg cggtgagctt
     1321 ctgcaacacg agcctcgcat cgccctccag gcagactttc gcggggtagt tcgcattgaa
     1381 caccgccggg ttgacgtcga cgtggatcag gttggccggc ggctcccagc cgtagctgcc
     1441 ggtcgcgatc tcgccgaagc gcgtgccgac cgcgagcatg cagtccgtgt gctcgaaggt
     1501 cttctcgacc gcgggcaccg ccgcacgtcc gaggcagaat ccggcgtgca gggggtgtgt
     1561 cgcggggaat gcgctcacgc cctgcagcgt cgtcgagacg ggggcgccga gtcgctcggc
     1621 gagcgccgcc acttcggcag acgcatcgac ggcaccccac ccgacgaaga tgctcgggga
     1681 ccgagcgttg gcgagcaggt ccgccgccgc gccgatgccg gccgccgtgc agcgcgcctc
     1741 ggggatcgcc ggcccctggt ggcgcggcat cggcgacgga cccgggtcgc ccgtcagcag
     1801 ctgcaggttc accgggatct cgacgaacac cggtcccggc tcgccgctgg tcgccacgcg
     1861 gacggcttcg tggatcgtcg ggatcacgtc ggcgtgccgc tcgacgagcc acgtgccctt
     1921 cgtgatcggc ttcagcagcg cgtgctggtt gacgtcgtgc agctggtact ggcggccggt
     1981 gtcgcggcgg atgccaccgg agatcacgag catcgggatg ccgtcgagga acgcttccgc
     2041 gatgccgctc gcggcgtgcg tgacgcccgc cgcgggcacg atgacgagcg tgccgaggct
     2101 ggtgccgctg cggctcaccg cgtctgccat gaacgcgcct ccgccttcgt gcgtgaccag
     2161 cacgggcgtg atcgtcgcgg agcgcgcgag ctcgtcgtag atctccgtgt tgtgcacgcc
     2221 cgggatgccg aacgtgtagc gcaccccggc gtgctcgagt gcctcgcgta gcaacgatgc
     2281 ggcggtccgg ctcacgacga ccatcctttc gctcggattg caagtcgagc gccgcgattc
     2341 taggcacagc gcacagttcg agccatccgc ttgacccgca ctcgctcggt cacccaagct
     2401 gcccggcccg ggcggatcgc gcgcagaacg gtctcccgcg gcagacatcg caaggagggt
     2461 gcgcccgagt ggacgaggtc aggatcgccg tgatcggggc gggcgtggcg ggtctcgcct
     2521 gcgcgcagga gctgctgcgg gccgacgcca gggtcacggt gttcgagcgc tcgcgcggcc
     2581 tcggcggacg gctcgccacg cgtcgccagg gtgacctcgc cttcgaccac ggcgcgcagt
     2641 tcatcacggc acgcagccgc gccttcgtcg gctacgccca ggccgccgtc cgtgccgacg
     2701 tgctcgacgc gtggcggccg cgcctcatgg aagacgatcg cagctggcct gcgccaatcg
     2761 aggactggtg gatcggcaag cccggcatga gcgcgctcgt ccgcccgctg gcgcgcaacc
     2821 tcgagataca caccggcgtc gccgtgcacg aattgctgcc gagccagcgc ggctgggaac
     2881 tccagaccga ctcgggccgg cagcaccagg tgtttcgcgc cgtggccgtg gcgattccgg
     2941 caccccaggc tgcgacgctg ctcggcccgc acgggcgggc gttccgccac atcgccgacg
     3001 tgcgcatggc gccctgctgg accggcatgt tcgcgttcga ccggccgctc gacctggggg
     3061 ccgaagcgcg acgctggacg gcggggcccc tggtctgggc cgcctgcaac tcgagcaagt
     3121 cacagcgact gcggcggccg caatgctggg tggttcacgc ctcgtcgagc tggtcccgcg
     3181 agcggctcga actcgacgcc ccggaagcgg cccgtcaact gttcgagacc ttcgcgcacg
     3241 ccgtcggccg cgcgttgccg gcgccctcgt tcagcacggc gcatcgatgg cgccacgccc
     3301 tcgtcgagca gccgctcggg ctgcagtcgg tggtggacga ggagatctcg gcgggcgcgt
     3361 gcggcgactg gtgcatcgcg ccgcgcgtcg aggccgcgta cgagagcggc cggtcactcg
     3421 ctcactcgat cctgtccatg gtggggctgt cggcgcggat gtcgcgcgcc tgaccgcgtc
     3481 gcggcaacag gcgtgagagc acgccgtcgc gcgagaatcc ctcgaacgcc gcccacagca
     3541 ccagcgcgca cagcgccggc acgatttcgt agatcacgcg atacatcagc acggcggcga
     3601 gcaactgctc gggcggcaca ctcggcaaca gcagcagcag catcgactcg agcacgccga
     3661 gacccgccgg cacgtgactc aacacgccgg ccagcacgct cgcgaggtac accgcgagga
     3721 acggcaggta accgaggccc gtgccctccg gcagcagcag gtacagcacc gtggaaacga
     3781 gtccgatgtc caccagtccc actacgacct gcagcgccgt catcgcgggc gtcggcaggt
     3841 gcacggccca gccgcccagc ttcacgggtt tcctgcgcaa ccagatcgtg agcgcatagc
     3901 ccacgtcctt ggcgaggccc gcgcagccga gcgcaaagag ccacggtgcc tggatccgga
     3961 acagcggctc gagcgtggtg gactccatca cgagcgcgag gtcgagcaac agtccgaatg
     4021 ccagtccgta cggcaggttc gcgagcagca cggtcgcgcc gacttcggtc gcggcaaaac
     4081 ccgccggcgt gtacatgcga taacgcaatg cgccaccgct cagcatcgcc tgcccgaccg
     4141 cgtgcccgag cggaaacgcg atgaacgcgg tgaccatcgg cttggcgcgt ccgatgcagt
     4201 gcttgacgta gcggacgacc gcgacttcgt agaacgcgag cgtggtgaac gcgccggcca
     4261 cgcagatcgc ggccagcgcg agcgtggacg cgggcaccgc gcgcatgttg gcgagcacgt
     4321 cggcgaagct gatgcgctgg aacgtccgcc acagcaccca ggccgcgagg ccggcgagca
     4381 ccgtgctcac tgccactccg agtggcttct tccagcgacc gcgacgggga tcgggcatgc
     4441 gcagtgatct cgagattgag cgccgcttcc ggccgcgggg cacgaccgct tgtgactcgt
     4501 caaacgcaat tgattctcaa ttcgcctgcg aggttttcgc tttgcaacga aacctgcctg
     4561 cgaccgcgcg gtccaatgcc aagcgcatcg accgtgcgta caatttcgcc tgcggccgat
     4621 tgtcccctcc cgaggagaag tatccaaatg tccgatcaga agttcccgcg tcgcaccctg
     4681 ctgaagggcg cgctcctggg cgtcgcggcg gtgccggtgt cggcgctcct ttcccgtacg
     4741 gccgacgcgg ccgccggcaa ggtcgacccg aacgagccgc aggcgaaatc gctcggctac
     4801 gtggaagacg cgaccaaggt cgatccgaag gccaacccca actacaagcc cgggcaggtc
     4861 tgcgccaact gcctgcaggc gccggccgcc aagtccggcg gtgccgaggt gccgtgcaac
     4921 atcttcgccg gccgcaacgt cgccgcgaag ggctggtgca aggtctacgt caagcgcccc
     4981 tgatcagcgg gtcgtgactt gatcgcgacg gcgcgcctcg ggaaaccggg cgcgccgttt
     5041 tcattgcggt gacgcgaccg tccggttccc gctcgccacc ctctggttcc gaccccttga
     5101 aacccccggc gccgcgcaca tctaaggcgg caagcaggat tgccatgcaa tcccgggcag
     5161 atcgatggat ggacgaatgg aggccaggac aatgcgagtg atcaagtggg aaccgttccg
     5221 cgacatggac gacgtgttca accgtttctt cgccgacacg ctgcgtcgct tcccgatgca
     5281 gtccggcgtg ggcgccggtc acgagggcgc ccagcagccc cgtgaatggg ccccgctcgc
     5341 cgacgtgagc gagaacgacg gcgagtacgt gatcaaggcc gaagtacccg aggtccgcaa
     5401 ggaagacgtc agcgtcaccg tgcaggacgg cgtgctcacg ctttcgggcg agcgcaagca
     5461 ggagcagcgc gaggagaacg agcgcgtgca ccgcatcgag cgcttccacg gcacgttcgc
     5521 gcgtcgcttc gcgttgcccg agaacgcgga cgaacagggc attcgcgccg agtgccgcga
     5581 cggcgtgatc gtgatccgca tcccgaagca gaagcaggtc gagcagcagc cgcggcagat
     5641 ccagatccag taaccacggt cggcccgcgg attgcgcgac gggtggcacg gatggccgtg
     5701 cctcccgtcc gcgtcgcgcc cggagtcgcc ggcacctata atcgccgccc gctcgctgcg
     5761 gatgcgacac acggacacca cggccatgac cacgagcaag tccctcgcca aggggacggc
     5821 ggacctctgt gacgagcacg gcgagcgcgt gcaggtctgc gaaccgatct tccaggctta
     5881 tggcggacgc aagtcgttcc acggggccgt cagcaccgtg cggtgcttcg aggacaactc
     5941 gcgcgtcaag gaagccgtcg aaggccccgg cgaaggccgc gtactcatcg tcgacggcgg
     6001 cggctcgcgt cgtcgcgcgc tgctcggcga caagctcggc gaggccgccg ttcgcaacgg
     6061 ctgggccggc gtgatcgtgt acggctgcat ccgcgactcc gccgaactgg gccgcatcga
     6121 cctcggcatc cgcgcgctcg gcaccatgcc gctgcgcagc gacaagcgtg gcgaaggcga
     6181 gcgcgacgtg cccgtccgct tcgcaggcgt cacgttccgc cccggcgagt tcgtgtacgt
     6241 cgacgaggat ggggtgatca cggcgcagga agcgctgact tgatcagtgt ctcgtgtcca
     6301 gtgtcgagcg gttagtgggt ccaggagggc tggcgtgcac cggcccgctt gactagacac
     6361 tagacactaa ccactggaca ctttcctcat gcgtaccgtc ttctgccagt acctccagcg
     6421 cgaagcgccc ggcctcgagc gcgtcccgta tccgggcgaa ctcggcaggc gcatctacga
     6481 caacatctcc gccgaagcct ggcaggcctg ggtgcggcac cagacgatgc tgatcaacga
     6541 ataccgcctc tcgccgatcg agccgaaggc ccgcaagttc ctggtcgagg aaatggagaa
     6601 gttcctgttc gccggcggcg cggagcggcc ggcggagtac gtggaacccg accaggaata
     6661 gtggctagtg gttagtggat agtgagcaac gtcgatcagg gctttgcttt cactagccac
     6721 tgaccactag ccactgacca ctctcctcgc gtgcaacgcc aggcacgccc acccgaccat
     6781 gaagctcacg cctcccagcg gcgcgaccat gccgagcgcg cgcggtgcgc caaacgtcat
     6841 cgcgtagatc gagccgcaga agagcacgat gccggcgccg aacagcgccg cggcgaccgt
     6901 gagccagcgc gtccgcggcg tcacgcacgc gacgagtccc agcagcgcca gcgcgagcgc
     6961 gtgcagctgc tggtaggtga ccccggtctg gaagctcgcg agccttcgcg gcgtgagctg
     7021 tgcctgcagc gcgtgggcgc cgaatgcgcc gagcatcacc gcgatcagca tcagcagcgc
     7081 cccggctccg atgcagcgca tcgcgaaccg gcgatccacg acgttgcgcc gcggctcgtc
     7141 cgtcaagccg catcctcggc gtcgggtcgt ggatccatcg cccgctgcag cagcgtggcg
     7201 accggcttcg gcaggccgag ctcggcgggc gcccccgtgt cgtaccagag gcagcggtcg
     7261 ccttcgcgca tcgcccgcgc ctggacgccg agacagtcga cgaagatcgg cttcatctcg
     7321 tagtcgaagt gactgaacgc gtggtgcagc ggattgccgg cgtgcggggc ggccggggac
     7381 gacaggtttt ccgaacacca ctgcaacgcg tgggcgcgcg tcgggaactc cgggaaggcc
     7441 cacaacccgc cccagaggcc gctcggcggg cgccgttcca gcagcacttc gcgaccacga
     7501 cgtgccacga cgagccagcc ctcgcggtgc ggtcgcggct tgcggggacg cggcgccgga
     7561 agagcgtgct ggcgttcgcc ggccagcgcg aggcagccgg cgttgaccgg gcacaacaga
     7621 caggcgggat tcgcgcgcgt gcagatggtc gcgcccaggt ccatgatcgc ctgcgtgtac
     7681 gcatcgacgc cgtcggccgg cgtgcaggcc tcggccaggg cccacagccg ctgttccacg
     7741 gccggttcgc cgggatatcc ctcgacgccg aagtagcggg cgagcacgcg cttgacgttg
     7801 ccgtcgagga tgggatggcg ctcgccgcgc gacaacgcga ggattgcgcc ggccgtggag
     7861 cgtccgatgc cgggcagctc catgacctcg tcgagcgtgg ccgggaactc cccaccgtgc
     7921 cgctgcacga tcgcctgcgc ggcgcgatgc aggttgcgcg cgcgggcgta atagccgagc
     7981 cccgaccaga gatgcagcac ttcgtcgagc ggcgccgccg cgaggcgtcg cacgtcgggg
     8041 aaccgcgcca tgaaccgctc gtagtacccg gccaccgtgg cgacctgggt ctgctgcaac
     8101 atgatctccg agacccacac gcgataggcg gtcgggtcgc gctgccacgg caggtgcttg
     8161 cgcccgtcct gctcgtacca ccgcagcagc gcgggcgcga actcgacgtc ggtcacgaca
     8221 cgcagacgac ggccgtgtcg ccgctccccg cgtggcaagc gtcgggaaag ccgtactgct
     8281 tccagtagtc gagcagcccc atgcgccgtg cgagttccgg gaagcgcgga tcgcggcgga
     8341 acgcggcgct ctcgatggac caggcgttca tgacgtccca cttgtgcgcc cacgcggtgc
     8401 ggttgcggtc gagcgcgtcg aacatgatgt cgtagacgat gtcgacctgg cccagttgca
     8461 ggtacggcat cagcaggtcg tcctgctcga cgatcgacgg gtcgatttct tgcatcgccg
     8521 cgacgacttt cggtcgcttc gccgggtcgg ccaccgcgtc gatccattgc gcgacgagcg
     8581 gcttgagttt ttccggcgtg tccccgtgct ggaacagcgt cttcgcgtcc tcccagcgcc
     8641 cctggcgcat tgcgatcatc gccgccacgc cgctgccctt gttcgtgagc ccgaggtcct
     8701 ctgcaagctt ggcgtagcgc gcggcctgct ggtcgtcgcc gcgcagcagg tacgcgttgg
     8761 ccaggttcga ggcgaggatc ggcgagctcg ggtccagctc gaacgcacga cgtgcctgct
     8821 cgagtccggc atcgaggcgg ccgacgctgt tgagcaggat cgagtaccag tggtgcggcg
     8881 tcggctcgtt tggatcgagc gagatcgcga agaagaaacc cgattccgcg tcgagcaggt
     8941 tgccacggtc ggagttgatc tgcgcgagca cggcgtgggc ttcgccgatc ttcgggtcga
     9001 tcgcgagcgc ctggcgggcc gactcctccg ccatcttcag gtacttctcc tcgtcgtcct
     9061 cttccttgct gtagccaggc agcacgacgt acgcagaggc gagcgcggcg tgcgcggcgg
     9121 caaagccggg atcgcggccg atcgcctgct ggtagagctc gaccgcccgg cgcaggttct
     9181 cctcgccgcg tcgcttccag atcgcctggc cctgtaggta gagctcgtag gcctcgacgt
     9241 cctgcgtcgg ggccttgtcg cgctgggcga actgctgttc gtccggggcg agctggatcc
     9301 tgaggcgctc cacgatcgcc tgggcgatct cgtcctggac gacgaagatg tccttgagct
     9361 cgcggtcgta ggtctccgac cagaggtgga agccggtctc ggtgtcgatc agctgcgcgg
     9421 tgatgcggac cttgtccccg gcctggcgca cgctgccctc gagcaccgtc tcgacgccga
     9481 gctcgcggcc gacttcgcgg atgtccacgt tgcggccctt gtacgcgaac gcggaggtgc
     9541 gcgcggccac ctgcaggccc gggatgcgcg cgagcaggtt gagcagctcc tcggacatgc
     9601 cgtcgctgaa gtagtccttg gccgggtcgc cgctcaggtt ggtgaatggc agcacggcga
     9661 tcgagctgtg tggctccccg gcctgcgcac gcgcgaagcc gcgctcgtac agcaggtagc
     9721 cgagcccggc catcgcgagc agcacgagca cgccgaacgc ggcccgcatg cgcacgcgag
     9781 actgcggtgc gtcaccgggc aggggggcgg tgcgctggat gccggtcggc gtcgcatcga
     9841 agatccaggc gaggaccatc gcgatcggga agccgaccag caggaacacg acgatgaacg
     9901 tgacggacca ttcaggaacg tgcaaggcgg gcagcacgac cgaagcgctt tcggtcacgc
     9961 cccacgcgac ggcgccgtac gcgatggcca ccttgccgac gcgccgccgc ttcaattccg
    10021 ccaagaacgc cattgtcctg cccgaaactc ccccggccct ggtgacggcc cggcttccgc
    10081 gcgcaccacg gaggtctgac cgcaacggac cgtcgtcgtt cgctccgagc gtattgtcca
    10141 tgtttcaggg acgggcgcga gcccgccgga tccagccgcg aggcgtcagt cgcggcacac
    10201 gccgatgtct ccgttcagcg tcttcacgcg cacccgggcc gcgctcgatc cctcctggaa
    10261 gcgcagctcg cgtcctggcg cgtattcgtc cgtgcgctgg gccttggggc cgaagcagtt
    10321 gcggatctcg ccgttgaacg tcgagaggtc gaactggccg gccacggcac cggcgaagtc
    10381 cagtcgcacg tcgccgctga tgctctcggc gtcgagacgc gcgtccgggc cgagctggcc
    10441 ggtcagcgtc aggtcgccgc tcgtcgagcg caggcgcgag cgcgagattt cgcccgcgcc
    10501 gaaatcgaac gagccgctca ccgtgttgcc gttgacctcg cccgcgacgc tcgtcacggt
    10561 cgcatcgccg ctcacggtcg tgatcgacac caggcccttc tggcccgaac cgctgatcgt
    10621 gacgtccccg ctcacggtgc ggcactcgac gtcctcgccg ctcgcttccg tgcgcacgtc
    10681 gccgctgacg gcctgcagcc gctgggcgcc gcgaacgccc ttcacgacga tgtccgcgct
    10741 gacggtgttg accgagagca cgctgccggc cgggactttc acgatcagct cggagtcgtc
    10801 gacgttgtat gaacgcttgg gcacgacgac cttgacgcgc gtcagcttct cggacttggt
    10861 gaactcgagc gtctcggtac cgtcgcccag ttcacccgtc acttcgacct cgttgcgatt
    10921 ccagccggtc accgtgacgc tgccggcgac gttcgacacc tcgacggtcc cgtgggggtc
    10981 ggcggtggtc cgcttgttga tgggcgtgcc ggcggcggcg atgcccggcc acgccgtgag
    11041 cagcgcgagc gcgcagctcc aggcagggag cgtccggcgg ggcatcgggg tcacgtcgca
    11101 agtcataact gcatcctctt cgggtccgcc ttcgggtcgg cggtgccggc cagggcaccg
    11161 gccagctggt tcacgttggc gagcacggcg agctcgtcct ggtacgtctt caacaacaat
    11221 tcctcgagca gcggatcacc gggctgctcc gctagcgccg cgttgatctg cagggcggca
    11281 cgacgcatct ccgcgaggtt gacctcgagc ttcgcgcgcg tcgactccgg caggttcgcc
    11341 atccgcgtgt cgagcagcgc cgtgagctgc cggcgtgcct cgaggtaatc cggatcgagt
    11401 cgagcgccgg cgccgaatgc ggccggcaca gccgcggccc ccgtgggcac cgtaccggcg
    11461 gccggtatcg tcgcggtcgc catggtgccg tcttcgccgc gggtgtccag cagcgttgcg
    11521 gtgagcagcg acgaacctgc gacgagcagc accgcggccg cggcctgcca ggcccacgct
    11581 cgtcgccgcg acggcgatcc cgcgacgcgc ggctcgagtc gcgcggcgac cgtgggccac
    11641 aggtcgtgcc cgggctcgat cccctggtac gacagccccc gcagctgcgc gtcgagcctt
    11701 gggtcgtcgc tgccgtcgcg tcgttcagtg tccgtcatgg tccgtctccc gtgccgcgag
    11761 tcgcgcgccc aatagtgcgc gcgcccggtg cagctgcgcc ttgcaggtgc ccaccgcgat
    11821 gccgagcatg cccgccgcct cctcgtgcga gtggccgtag acgccgacca gcacgagcac
    11881 gtgcctggcg ccgcgcggca ggctcgcgat cgccgcctcg aggtctcggc cgctcgccgg
    11941 atcggacgcc gcatgatcga ccatcacgtc tgccacttcg cgctccaccg agtcctccat
    12001 gccgagcgat tcccgccgcc gccgggcctg ctgcagcacc gcgttgaccg cgatccggtg
    12061 cagccaggtg ccgaaagcgc tgcgcgtctc gaaccgcggc aggtgcttcc aggcctgcac
    12121 gaaggcctcc tgcgtgcagt cctcggccgt cgcgacgttg ccggtcatgc gcaggcacag
    12181 tgcgtggatc cgcgcgacgt gcccccgata gagggcctcg aacgccggca cggagccctc
    12241 gaccgcggct gcgacgagcc ggcgctcctc ggccgcctgc cgcgcacgct gcagttcatc
    12301 gacgacctcc tcgcccttcg gcaagaccca cctcgcatgc cccgtcgcac ggcgcgtccg
    12361 ggtggggcat cctacacagg ccccagccgg ggccatgccc tcgcaggtac agatgcgcgg
    12421 gcgcgacaaa aggtttgagg gggatgccga cgatgactga tgccgtccgc gtggaccggg
    12481 ccggtccggt cgccaccgtg gtcctggaac gcccggcggt ccgcaacgcc gtcgacccaa
    12541 ccaccgccgc cgcgctggcg gccgcctttg cctcgctcga cgatgatccc gccattcatt
    12601 cgatcgtcct gtggggcgcc ggcggaacgt tctgtgcggg cgccgacctg caggccgtcg
    12661 cgcaggggtg ggaccctgca cggctgcggg cgccggacgg ctcgccccgg gatgggtacg
    12721 ggccgatggg accgacgcgc atggaactcg gcaagcccgt gatcgccgcc gtggccggtc
    12781 atgccgtggc gggcggactc gagctcgcac tctggtgcga cctgcgcatc gccgaagccg
    12841 atgccctctt cggcgtctac tgccggcgct ggggcgtacc gctgatcgac gggggcaccg
    12901 tgcgactgcc gcgcctcgtg ggcgccggtc gggccatgga catgatcctg accggccgtg
    12961 cggtcggcgc cgaggaagca ctcgcgatcg gactcgtcga ccgcgtcgtg ccgaacggcc
    13021 gatcgcgcga ggctgccgag caactcgccg ccgagatcgc ccggttcccg cagggttgcc
    13081 tgcgcgccga tcgcgcgtcg gccctcgcgc agtggagcct gcccgtcacc gccgcgctgg
    13141 cgcgcgagta cgagtccggg ctcgcggtgc tgcgcagcgg cgagtccgcg cgcggggccg
    13201 cgcggttcgt ctccggtgcc ggacgtggcg gccgacccgc ctgatatcca atcgatccaa
    13261 caggagttcc aactcatgcc gatccagagc ccgatcttcg cgcccgccac cgcgctggtg
    13321 ctgtggtccc tcgtgatgct gggctggctc gcgctgacgc gcatcccggc gatgacgaag
    13381 gccggcatcc cggtcatgaa gatcgtgggc agccgcggcg tgaacctcga aggcgtcgta
    13441 ccggaccctg tgaactggaa gtcgcacaac tacgcgcacc tgatggagca gccgacgctc
    13501 ttctacgcga cggtcgtgat cctcggcgtc gtcggcgccg gcgacggcct gaacctgcag
    13561 ctggcgtggg cgtacgtcgt cctgcgcgtc gtgcacagca tcgtgcaggc cacctggaac
    13621 cgcgtggccg ttcgcttcac gttgttcgcg ctcgcgacgg cggtgctcgc cgtgctcgcg
    13681 atcaacgcgg cgcggctggt gttctagcgg ccttcgaggt agtcgagcgc gagcgccgtc
    13741 agggtcctga cgccgatcgg caacgccgct tcgtcgagga agaaccgcgg cgagtggttg
    13801 ttcggcgccg tcgcgggatc ctgcccctgc ggtgtcgagc ccacgaagta gaagaacgac
    13861 gggacctgct gcccgtagta ggcgaagtcc tcggatacgg tcaggtacgg catcaccgcg
    13921 acctggtcct tgccggtcac acgctcgagt gtcgtcaccg cgcgcgcagt gagcgacgga
    13981 tcgttcacta ccacggggtt cgggtcgggc cccagttcga actcggccgt tgcacccgcg
    14041 gcggtcgccg tgtcggtcgc gatgcgccgg atgcgctcga tgacctgttc gcgcaccgcc
    14101 gggtcgaacg tgcggaaggt gcctaccatc tcgaccgagt ccgggatgat gttgttgcgg
    14161 atgccgccct tgatcgcgcc gaccgaaacg acggccgggt actgcgcgat gtcgacctgg
    14221 cgcgcgacga tcgtctgcag gcccgtgacg acctgtgcgg ccgtgacgat cggatcgacg
    14281 ccgccccacg gccgcgcgcc gtgcgtctgc cggccccgga ccacgatgcg gaaccgatcg
    14341 gacgcggcca tgagcgggcc cgtgcggtag ccgacctggc cgacgttcag cgtcgaaaac
    14401 acgtgcaggc cgaacatcgc ctcgggcttc gctgcggcaa acaacccctc gtcgagcatc
    14461 aggctcgcgc cgccgcgctc cccgtcgggc gcaccttcct cggccggctg gaacacgaac
    14521 acgactgtac cgggcagcgc gttgcgcatg tcgccgagga tccgcgcgac gccgagcagg
    14581 atcgcggtgt gggcgtcgtg gccgcaggcg tgcatgacgc cgaccttttc gccgcggtac
    14641 tcggcggtcg cgaccgactt gaacggcacg tccacctgct cggtgacggg cagcgcgtcc
    14701 atgtcggctc gcaacgcgat ggtcggcccc ggtttggccc ccctgaggat gcccacgacg
    14761 cccgtgtgcg caatcccggt cctcacctcg aagcccagcg tccgcagttg ctcggcaacc
    14821 accttggacg tgcgcacctc gcggttgctg agctcggggt tctggtggaa atcacggcgc
    14881 caggtggtca cctgcggcat caccgatttc atcgccgcat cgagggccgc atcgtcggag
    14941 gccgcgccgg ccgggccgca cgcgagcagc gcgaagggag cgaggcggag caacttcagc
    15001 atgacgaccc ctgtggcacc cgacgagggc gggcagcata ctgcagcgac cgcatcgaca
    15061 gcgacagcga gacgttccca tgagccagcc cacgaagtcg gtcgcggtcg tcgccgcact
    15121 gagcctgttc tgcatgacca ccgcaccgtc ttcttcgaac gcccaggccc gcgccgcgcc
    15181 gacgccgccg gccggccagg ccgtctcacc gagcgtcccg cgctcgatct cgaagacgca
    15241 gaagccgccg ctgcatgggc agcgctggat ggcgatcacc ggcaagccgc tcggcgccac
    15301 ggccggcgcg atgatgttcc agcgcggcgg caacgcggtc gacgccgcgt gcgcgatgat
    15361 cgcggcgacg tcgacgatgt gggacgtgct gagctggggc ggcgagacgc aggcgctgat
    15421 ctacgacccg cgcgccaaga aggtcgtcgc gatcaacggg ctcggcgtcg cgccgagcgg
    15481 tgcgaccgcc gagttctatc gcggcaaggg ctacaagtat ccacccgagc acggcccgct
    15541 cgcggcggtc acgccgggca cgcccggtgg tgtgatgctg atgctgcagg agtacggcag
    15601 gctctcgctc gccgaggtgc tgcagcccgc gatcgagctc gccgacggct acccgatcga
    15661 tg
//
LOCUS       DASPFR010000345         3613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1811992, whole genome shotgun sequence.
ACCESSION   DASPFR010000345 DASPFR010000000
VERSION     DASPFR010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1811992"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..597)
                     /locus_tag="VD737_09135"
     CDS             complement(28..597)
                     /locus_tag="VD737_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HXW10767.1"
                     /translation="MDKTFITASDLLQDSFRLAAQIHRSGFRPDFIIGIWRGGTPVGI
                     AIQEYFEYVGVRPDHIAVRTSAYVGIGERAKTIRVHGLHYIVDEADADDSLLIVDDVF
                     DSGHSIEALLAELATKMRLNLPRQIRIATPWYKPGNNRTSLVPDYYLHETDAWLVFPH
                     ELGGLTYDEIKAGKPELAAVVDLLQPPKA"
     gene            740..1228
                     /locus_tag="VD737_09140"
     CDS             740..1228
                     /locus_tag="VD737_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXW10768.1"
                     /translation="MTVKRMDNVGIVVEDLDAAIGFFTELGLGLEGRAPVEGDWADGV
                     TGLRGMRVEIAMMRTPDGHGRLEISRFVAPPVVSDHRNAPVNALGYLRVMFTVADIDD
                     TLTRLRQRGAELVGTVVQYADMYRLCYIRGPEGILIGLAQQLEQQTTRVDPLRRSGDS
                     QA"
     gene            complement(1231..2055)
                     /locus_tag="VD737_09145"
     CDS             complement(1231..2055)
                     /locus_tag="VD737_09145"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HXW10769.1"
                     /translation="MTGMVTLAGLFVYPVKSCRGTPLAHGTLTKSGLMHDREWMVVTP
                     AGRFLTQRELPRLALVSPQLRADALVLDAPGMATLSLPLDDRSGARRAVVVWRDACQG
                     IDAGDEAADWFSVFLGRPVRLVRFDESQRRATDPHWSAGIEAETRFTDGFPLLVLSRA
                     SLDDLNSRLLHPLPLDRFRTSLLIDDCAAYAEDRMATLEAGGVRIRIVKPCARCVITT
                     TDQSTAERDGEEPLRTLKTYRWDPELKGVVFAQNAVVEAGAGAQLSVGMTLDVVWK"
     gene            complement(2083..>3613)
                     /locus_tag="VD737_09150"
     CDS             complement(2083..>3613)
                     /locus_tag="VD737_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXW10770.1"
                     /translation="ADADRSYRDLVERIGQGAVSLTHRGEVLYANEPFGAMLGLRRED
                     VLGRDFLALVEDGAGRLQAFLADPAPERGTMELTLRRKGGEPLPVAVSAEVVPGRDVA
                     ATLVLTDLTERRRHVKIQEETRRKDEFLAVLAHELRNPLASIRTCVQILEGSKALAGH
                     DQQAVQIIGRQGATLVRLVDDLLDIHRLNEGKIVVRREPIAVREIVDDAVDAARPYLT
                     TGRLRLEVRTPEQALHVEGDKVRLVQVLLNLLSNAAKFTPRDGLVCVDVEPVAALAGR
                     PAAARITVTDNGRGIEATQLESIFEPYVQLGTAGETPASGLGLGLSVARRLVELHGGT
                     IRAESAGSDRGSRFLLELPTCQASSIDASATPEAAPEAAGPRLRVLIADDNRDAAESL
                     AMLVRLSGHDVRTVHDGRQALTAGESFRPDIVFLDIGMPVLDGYDTARSWRAARWGEG
                     SKLYALTGYGQGEDRQRRSREAGFDGHFVKPIDPDHVLKLLQDTSGAPPASDRTAAAA
                     N"
BASE COUNT          530 a         1247 c         1258 g          578 t
ORIGIN      
        1 gggctccccg cgacggccgc cgtccgatca ggctttcggg ggttgcagca ggtcgacgac
       61 ggccgcgagc tccggcttgc cggccttgat ctcgtcgtag gtcagtccgc cgagctcgtg
      121 aggaaagacg agccaggcgt cggtctcgtg caggtagtaa tccggcacga ggctcgtgcg
      181 gttgttgccc ggcttgtacc agggcgtcgc gatccggatc tgccgtggca ggttcaggcg
      241 catcttggtc gcgagctcgg ccagcagggc ctcgatgctg tggcccgaat cgaagacgtc
      301 gtccacgatc agcagcgaat cgtccgcgtc cgcctcgtcg acgatgtagt gcaggccgtg
      361 cacgcggatc gtcttggccc gctcgccgat gccgacgtac gcggaggtgc gcaccgcgat
      421 gtggtccgga cgcacgccga cgtattcgaa gtactcctgg atcgcgatgc cgaccggcgt
      481 gccgccccgc cagatgccga tgatgaagtc cggtcggaac ccgctgcggt ggatctgcgc
      541 cgcgagccgg aaggagtcct gaagcaggtc gctcgcggtg atgaaggtct tgtccatggc
      601 gcgatgatcc gtcggaacgc cggcttcgtg gaagacggta actgccgcag tgcgtgaccg
      661 cgggtccaga ccgtcggcag ctgcggcagt gataggctgc cgtcaacggc cgggcatcgc
      721 tgcgcgccga ggaggcatcg tgacggtcaa gcgcatggac aacgtcggca tcgtcgtgga
      781 agacctcgac gctgccatcg ggttcttcac cgaactcggt ctcgggctcg agggtcgtgc
      841 cccggtcgag ggcgactggg cggacggcgt caccgggctg cgcggcatgc gcgtcgagat
      901 cgccatgatg cggacgcccg acggccacgg ccggctcgag atctcccgct tcgtcgcgcc
      961 gcccgtggtc tcggatcatc gcaacgcgcc ggtgaacgcg ctcggttacc tgcgcgtcat
     1021 gttcaccgtg gctgacatcg acgatacgct cacccggctc cgccagcgcg gcgcagaact
     1081 cgtcggtacg gtggtccagt acgctgacat gtatcggctc tgctacatcc gggggccgga
     1141 gggcatcctg atcggactcg ctcaacagct cgagcagcag acgacccgtg tcgatccctt
     1201 gcgacgcagt ggtgactctc aggcgtagcc ctacttccac acgacgtcga gcgtcatccc
     1261 gacgctcagc tgcgcgccgg ccccggcttc cacgacggcg ttctgcgcaa acacgacgcc
     1321 tttcaactcc ggatcccagc gataggtttt cagggtcctg aggggctcct cgccgtcgcg
     1381 ctccgccgtc gactggtcgg tcgtcgtgat cacgcagcgt gcgcagggct tgacgatccg
     1441 gatgcgcacg ccgcccgcct cgagcgtcgc catacggtct tccgcgtagg ctgcgcagtc
     1501 gtcgatcagc aggctggtgc ggaagcggtc gagcggcagc ggatgcagca ggcgcgagtt
     1561 caggtcgtcg agtgacgcac gcgacagcac cagcagcgga aacccgtcgg tgaagcgcgt
     1621 ctctgcctcg atgccggccg accagtgcgg gtcggtggcg cggcgttgcg actcgtcgaa
     1681 acgcaccagt cgcaccgggc gtccgagaaa gacgctgaac cagtcggccg cctcgtctcc
     1741 cgcgtcgatg ccctggcacg cgtcgcgcca gacgacgacg gcgcggcgcg cgccggagcg
     1801 gtcgtccagt ggcagcgaca gcgtcgccat cccgggcgcg tcgagcacga gcgcgtccgc
     1861 gcgcaactgc ggcgacacga gcgcgagcct cggcagctcg cgttgcgtca ggaatcggcc
     1921 ggccggcgtg acgaccatcc attcgcgatc gtgcatcagc cccgacttcg tgagcgtgcc
     1981 gtgggcgagc ggcgtgccgc gacaggactt gacggggtag acgaacagac cggcgagcgt
     2041 gaccatgccg gtcattgtgc cagctgcgcg cgacgctttc gttcagttgg cggcggccgc
     2101 ggtcctgtcc gacgcgggcg gcgccccgga ggtgtcctgc agcagcttca ggacgtggtc
     2161 agggtcgatc ggcttgacga aatgcccgtc gaacccggct tcgcgcgagc gacgctgccg
     2221 gtcctcaccc tgtccatagc cggtgagcgc atagagcttc gagccctcgc cccagcgcgc
     2281 cgcgcgccag ctgcgcgccg tgtcgtaccc atcgagcacc ggcatgccga tgtcgaggaa
     2341 gacgatgtcg ggccggaagc tttctccggc cgtcagcgct tgcctgccgt cgtgcaccgt
     2401 acgcacgtcg tgcccggaga ggcgcaccag catcgcgagg ctttccgcgg cgtcgcgatt
     2461 gtcgtcggcg atcagcacgc gcaggcgcgg gccggctgct tcaggcgccg cctccggtgt
     2521 cgcgctcgca tcgatcgagg atgcctggca cgtcgggagt tcgagcagga agcggctgcc
     2581 acgatccgac ccggcacttt cggcgcgtat ggtgccgccg tgcagttcta caagccgccg
     2641 cgcgaccgac agccccagcc cgaggccact cgcaggggtc tccccggccg tgccgagttg
     2701 cacgtacggc tcgaaaatcg attcgagctg cgtcgcctcg atgccgcgtc cgttgtcggt
     2761 cacggtgatc ctggctgcgg cgggacgacc ggcaagcgcc gcgacgggtt cgacgtcgac
     2821 acagaccagg ccgtcgcgtg gcgtgaattt cgccgcgttc gacagcaggt tcagcagcac
     2881 ctgcaccagc cgcaccttgt cgccctcgac gtgcagcgcc tgctcgggcg tccggacttc
     2941 gagtcgcagc cgacccgtgg tcaggtacgg ccgcgccgcg tcgacggcgt cgtcgacgat
     3001 ctcgcgcacg gcgatcggtt cgcgacgtac gacgatcttg ccctcgttga gccggtggat
     3061 gtccagcagg tcgtcgacca gtcgcacgag cgtcgcgccc tggcgcccga tgatctgcac
     3121 ggcctgctgg tcgtgcccgg cgagggcctt cgaaccctcg aggatctgca cgcaggtgcg
     3181 gatcgaggcg agcggattgc gcaattcgtg cgcgagcacg gccaggaact cgtccttccg
     3241 gcgcgtctcc tcctggatct tgacgtggcg ccggcgctcg gtgaggtcgg tcagcacgag
     3301 tgttgcggcg acgtcgcgac cgggcaccac ctccgccgag acggcgaccg gcaacggctc
     3361 gccgcccttg cggcgcagcg tcagttccat cgtgccgcgc tcgggcgcag ggtcggcgag
     3421 gaaagcctgc agccgccccg cgccgtcctc gaccagcgcg agaaagtccc ggccgagcac
     3481 gtcctcgcgg cgcaggccga gcatcgcccc aaagggctcg ttggcgtaca gcacctcgcc
     3541 gcggtgggtc aggctcacgg ccccctgtcc gatgcgctcg acgaggtccc ggtacgaccg
     3601 gtccgcgtca gcg
//
LOCUS       DASPFR010000346         3514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_348539, whole genome shotgun sequence.
ACCESSION   DASPFR010000346 DASPFR010000000
VERSION     DASPFR010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348539"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..2091
                     /locus_tag="VD737_09155"
     CDS             133..2091
                     /locus_tag="VD737_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HXW10771.1"
                     /translation="MVVELGLFALVLELLLSIAQAFFGLAGPHVRNRRWMDAVRPAAA
                     GQWVFVALSFLVLAWAFYVNDFSVLYVANNSNSALPTMYRLTAVWGAHEGSLLLWAFA
                     LSSWSIAVAFFSRHLPQTFTSRVLGVLGVVSIGFQSFILFTSSPFERLIPAAMDGQDL
                     NPLLQDPALAIHPPMLYLGYVGFSVAFAFACAALLEGKLDEKWARWARPWTTVAWMLL
                     TGGIALGSWWAYYELGWGGWWFWDPVENASFMPWLVGTALIHSLAVTEKRGLFKSWTL
                     LLSIVAFSLSLLGTFLVRSGVLVSVHAFASDPSRGLFILAFLGICVLGSLGLFMWRGP
                     LLRSKGGFETTSRESFLLFNNVLLLVAALLILIGTLYPLFLDALNMGKISVGPPYFNL
                     VFLIPTLPMLLLLAVGMHSVWKVGRLDDKKTPLLAMLAAAVVLGVVIPIVAYHGFHPM
                     ASVGLAAGLWVIFSSLYDPIQRLRKRQALTSGVLGMTVAHIGVGLFAIGVTITQSYRI
                     EKDYALQPGESVEMQGYTFRFDSIEHVTGPNWDAEQAHFTVTRGDDLVAKLHPQKRVY
                     RVRTMPMTEAGIEVDWNRDLFVAMGEDLGANKWSMRLQYKPMVRFIWFGCLVMMIGGF
                     IAILDRRFRARRQEADERLGAGGTVKAT"
     gene            2102..2656
                     /locus_tag="VD737_09160"
     CDS             2102..2656
                     /locus_tag="VD737_09160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HXW10772.1"
                     /translation="MSNWLKYGIPLALCALLGIFLFVGLQRDPSYVPSPLIGKPAPDF
                     SLPSLQDASYPVSPSDLRGRPWVLNVWATWCGGCRQEHDTLIAIARTNVVPMIGLNWK
                     DDNAAAQQWLRALGNPYSAVAEDVEGRAAIDWGVYGAPETFLIGPDGTVLFKHIAPMT
                     MEVWTQEFLPRIQAAQAGAPASRG"
     gene            2659..3105
                     /locus_tag="VD737_09165"
     CDS             2659..3105
                     /locus_tag="VD737_09165"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HXW10773.1"
                     /translation="MNTSLTTFVRTLLAALLFAFAGHALAIDTEKAFDDPALQARYEH
                     INRELRCLVCQNQTIADSNATLAQDLRREVREMITAGRTDDEIREFMIARYGDFVLYR
                     PRMTAGNFLLWAAPVLLLLLGGFIGIRYVRRQAAEIDADRPEAGQS"
     gene            3105..>3514
                     /locus_tag="VD737_09170"
     CDS             3105..>3514
                     /locus_tag="VD737_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10774.1"
                     /translation="MSVFVVLCAVMALVAIFLVAYPLLKPLPPEAKGATPAPKAAPLA
                     FAVSVILALGAVGLYASINNYPWQNPQIAAAVPPGHGQAGQGVSMQEATAALEAKLAA
                     NPNDAEGWRMLGRTYLVSGEAARAAGAYERARSLS"
BASE COUNT          498 a         1213 c         1173 g          630 t
ORIGIN      
        1 gaggtcaagg acagcctgaa gccgcacatg gacgcggcca acaacatgcc gcagaacgac
       61 gcggcggccg gtaccggtta ctgagccgcg cgacactcga ctgactgacg tccgaatcgg
      121 agcacccgac gcatggtcgt agaactcggt ttattcgcgc tggtcctgga gctgctgctg
      181 tcgatcgcgc aggcgttctt cggcctggcg gggccgcacg tccgcaaccg acgctggatg
      241 gacgccgtgc gtccggcggc ggccggccag tgggtattcg tcgcgctgtc gttcctcgtg
      301 ctcgcctggg cgttctacgt caacgacttc tcggtgctct acgtcgcgaa caactcgaac
      361 tcggcgctgc cgacgatgta ccgcctcacg gcggtctggg gggcgcacga gggctcgctg
      421 ctgctgtggg cgttcgcgct gtcgagctgg tcgatcgcgg tcgcgttctt cagccgccac
      481 ctgccgcaga cgttcacctc gcgcgtgctc ggcgtgctcg gtgtcgtcag catcggcttc
      541 cagtcgttca tcctgttcac gtcgagcccg ttcgagcgcc tgatcccggc cgccatggac
      601 ggccaggacc tcaatccgct gctgcaggac ccggccctcg cgatccaccc gccgatgctg
      661 tacctcggct acgtcggctt ctcggtcgcg ttcgcattcg cgtgcgcggc gctgctcgag
      721 ggcaagctcg acgagaagtg ggcgcgctgg gcgcgaccgt ggaccacggt ggcctggatg
      781 ctgctgaccg gcggcatcgc gctcggcagc tggtgggcct attacgaact cggctggggc
      841 ggctggtggt tctgggatcc ggtcgagaac gcctcgttca tgccctggct cgtcggcacc
      901 gcactgatcc actccctcgc cgtgaccgaa aagcgcggcc tgttcaagag ctggacactg
      961 ctgctctcga tcgtcgcgtt ctccctgagc ctgctaggca cgttcctcgt gcgctcgggc
     1021 gtgctcgtgt cggtgcacgc gttcgcgagc gatccgagcc gcggactctt catcctggcc
     1081 ttccttggga tctgcgtgct gggctcgctg ggcctgttca tgtggcgcgg gccgctgctg
     1141 cgaagcaagg gcggcttcga gacgacttcg cgcgagtctt tcctgctgtt caacaacgtc
     1201 ctgctgctcg tcgcggcgct gctgatcctg atcggcacgc tctatccgct gttcctcgac
     1261 gcgctgaaca tgggcaagat ctcggtcggc ccgccgtatt tcaacctcgt gttcctgatc
     1321 ccgacgctgc cgatgctgct gctgcttgcg gtcggcatgc actcggtctg gaaggtcggc
     1381 cggctcgacg acaagaagac gccgctgctc gcgatgctgg ccgcggccgt cgtgctcggc
     1441 gtcgtgatcc cgatcgtcgc ctatcacggc ttccatccga tggcctcggt cgggctggcc
     1501 gccggtctct gggtaatctt ctcgtcgctc tacgacccta tccagcggtt gcgcaagcgc
     1561 caggcgctga cctccggcgt gctcggcatg acggtcgccc acatcggcgt cggcctgttc
     1621 gcgatcggcg tgacgatcac ccagagctac cgcatcgaaa aggactacgc gctgcagccg
     1681 ggcgagtcgg tcgagatgca gggctacacg ttccgcttcg acagcatcga gcacgtgacg
     1741 gggcccaact gggacgccga gcaggcgcac ttcaccgtga cgcgcggcga cgacctggtc
     1801 gcgaagctgc atccccagaa gcgcgtctac cgcgtgcgca cgatgccgat gaccgaggca
     1861 ggaatcgagg tcgactggaa ccgcgacctg ttcgtcgcga tgggcgagga cctcggcgcc
     1921 aacaagtgga gcatgcgcct gcagtacaag ccgatggtgc gtttcatctg gttcggctgc
     1981 ctcgtcatga tgatcggcgg cttcatcgcg attctcgacc ggcgcttccg tgcccgcagg
     2041 caggaggccg acgagcgcct cggtgccggc ggcaccgtga aggccacctg acggtgcact
     2101 catgagcaac tggctcaagt acggcattcc gctcgcgctg tgcgcgctgc tcggcatctt
     2161 cctgttcgtc ggcctgcagc gcgacccgtc ctacgtaccc tcgccgctga tcggcaagcc
     2221 cgcgcccgat ttctcgctgc cgagcctgca ggacgcgtcg tatccggtga gcccgagcga
     2281 cctgcgcggg cgtccgtggg tgctcaacgt ctgggcgacc tggtgcgggg gctgccgtca
     2341 ggagcacgac acgctgatcg cgatcgcgcg cacgaacgtc gtgccgatga tcgggctcaa
     2401 ctggaaggac gacaacgcgg ccgcgcagca gtggctgcgt gcgctcggca atccgtactc
     2461 cgcggtcgcc gaggacgtcg aggggcgtgc cgcgatcgac tggggcgtct acggcgcccc
     2521 ggagactttc ctgatcggtc ccgacggcac ggtgctcttc aagcacatcg caccgatgac
     2581 gatggaggtc tggacgcagg aattcctgcc gcgcatccag gccgcgcagg ccggcgcacc
     2641 ggccagccgg ggatgaccat gaacacgtcg ctcaccacgt tcgtccgcac gctgctcgcg
     2701 gcgctgctgt tcgccttcgc cgggcacgcg ctcgcgatcg acaccgaaaa ggcgttcgac
     2761 gatccggccc tgcaggcgcg ttacgaacac atcaaccgcg agctgcgctg cctggtctgc
     2821 cagaaccaga ccatcgccga ctcgaacgcg acgctcgcgc aggacctgcg gcgcgaagtg
     2881 cgcgagatga tcaccgccgg ccgcaccgac gacgaaatcc gcgagttcat gatcgcgcgc
     2941 tacggcgatt tcgtcctcta caggccgcgc atgaccgcgg gcaacttcct gctctgggca
     3001 gcccccgtgc tgctcctgct ccttggtggg ttcatcggca tccgctacgt ccgtcggcag
     3061 gccgccgaaa tcgacgcgga tcgcccggag gccggtcagt cgtgatgtcc gtattcgtcg
     3121 tcctgtgcgc cgtgatggcg ctcgtcgcga tcttcctggt cgcgtacccg ttgctgaagc
     3181 cgctcccgcc cgaggcgaaa ggcgcgacgc ctgcgcccaa ggcggcgccc ctcgcgttcg
     3241 ccgtgtccgt gatcctggcg ctcggggccg tcggcttgta cgcgtcgatc aacaactatc
     3301 cgtggcagaa cccgcagatt gccgccgccg tcccgccggg ccacggccag gcgggccagg
     3361 gcgtgtcgat gcaggaggcg acggcggcgc tcgaggcgaa gctcgccgcc aacccgaacg
     3421 acgccgaggg ctggcggatg ctgggtcgca cgtacctggt gtcgggcgag gcggcccgcg
     3481 ccgccggtgc gtacgagcgg gcccggtcgc tgtc
//
LOCUS       DASPFR010000347         4949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1812358, whole genome shotgun sequence.
ACCESSION   DASPFR010000347 DASPFR010000000
VERSION     DASPFR010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812358"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            393..698
                     /locus_tag="VD737_09175"
     CDS             393..698
                     /locus_tag="VD737_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine-zipper protein"
                     /protein_id="HXW10775.1"
                     /translation="MKTLTVAAAAIMVATATGCTSKDTLARIDAANAAAKQAASDAAA
                     AKQSADAAAAAASSAQNAASSAQSTANQALQASQASQSCCDATNEKIDRAFKRSMGK"
     gene            complement(808..1851)
                     /locus_tag="VD737_09180"
     CDS             complement(808..1851)
                     /locus_tag="VD737_09180"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXW10776.1"
                     /translation="MHRSNGSRGLVPVLVALVAPLVAGEAGAGAAEYLLPTNGDNVIG
                     VVTTAVAEHEDTLLDIGRRYGVGYEEIIAANPGIDPWLPGAGTEVVIPGRFILPDAPR
                     EGIVVNLPEHRLYYFPPAQPGQPRVVRTYPISTGKMDWKTPLGVTKVASKQERPNWNP
                     PESVRLEHIAKGDPLPKVVPPGPDNPLGEYAMRLAIPSGAYLIHGTNRPAGVGMQVTH
                     GCIRMFPEDIAEFFTMVPVSTKVNLIDQTTKVGWSRGTLYVERQAPLEGTQDPTHRDP
                     AELARVVASATAGTPVQVDWVTARQAFQQMSGVPVRIGMRDYARPVTDPPAPAAEPTE
                     VARHVEGERGATR"
     gene            1983..2510
                     /locus_tag="VD737_09185"
     CDS             1983..2510
                     /locus_tag="VD737_09185"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HXW10777.1"
                     /translation="MGYKLTIELSDRDLRHFRRELHKARESVQIADDEEILGAAADLV
                     AAMRRTDLPDFVTERLEKLETLLAMVMDHQWPLTDEERSPVLSGLAYLCDPEDLIPDD
                     IPGIGMLDDAVMIELVFRELRHELDAYADFLKFREQLVKGGGRPPTPPVEAKLERRRR
                     QLLERMRRRRKVASG"
     gene            complement(2592..3608)
                     /gene="plsX"
                     /locus_tag="VD737_09190"
     CDS             complement(2592..3608)
                     /gene="plsX"
                     /locus_tag="VD737_09190"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HXW10778.1"
                     /translation="MNAGQLTVAVDAMSGDHGAEVAVPAALEVLAVTADLRLIIVGRG
                     EVVRPLLGRRGETERCRFVEATEVVGMDERPQDALRRKKKSSMRVAINLVEQGEAAAC
                     VSAGNTGALLATSRFVLGMIPGIDRPAIVSAVPAVHGHTVMLDLGANPDCTAGNLVQF
                     AVMGSVIAADLHGVANPRVGLLNVGEEDIKGTEEIKEAHKRLQSTAALNYCGFVEGDD
                     IFSGGVDVVVTDGFSGNVSLKTMEGLARFISGVMKEEFTRDPLRKLGALAATPALNAL
                     KGRLDPRGYNGASMVGLNGVVIKSHGGADRVSFANAVRVAITEARNGVPGQIEQLLGK
                     VISG"
     gene            complement(3632..3826)
                     /gene="rpmF"
                     /locus_tag="VD737_09195"
     CDS             complement(3632..3826)
                     /gene="rpmF"
                     /locus_tag="VD737_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019342480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HXW10779.1"
                     /translation="MAVQKSRKTPSKRGMHRSHDHLRGPALSVDRTSGETHARHRITP
                     DGFYRGKKVIEKPATDEGQE"
     gene            complement(3848..4405)
                     /locus_tag="VD737_09200"
     CDS             complement(3848..4405)
                     /locus_tag="VD737_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HXW10780.1"
                     /translation="MSPGPADQVNAKELSARAAVVERRLELSHLDRVRDAGGLPGTRI
                     DAQLRFGAFDGRTTVAVLVDGVATLACQRCLQPCTTHVDESAQVMVVRDEDEDVPGGF
                     EPFVGLPEQLSLAALVEEQVLLALPLVPMHAADDPQCLANIDGAGSAPVRPLAADPPA
                     AGAAAEEKQTPFANLRELLEKKNER"
     gene            4465..>4949
                     /locus_tag="VD737_09205"
     CDS             4465..>4949
                     /locus_tag="VD737_09205"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HXW10781.1"
                     /translation="MFSKGLILASTSRYRRSLLGRLGLPFDCVAPEVDETRQPDESAV
                     GMAARLARLKAEAVSRLHPSAVVIGSDQVALRGHDLLGKPGTVEHCREQLQRSSGRDV
                     VFLTAVHVIDGPQGRAESHVDRTTVLFRELSATEIDRYIERDRPLDCAGGFKAESLGI
                     A"
BASE COUNT          757 a         1741 c         1619 g          832 t
ORIGIN      
        1 tactgtgctt atatacaggt cgcggtcatg cctgacaagc gggtgcggac ccctcgcctc
       61 tcccgtttgc gcggatgcaa cgcgagagtc cgcggcgcgg gccgacggct ggaatttgca
      121 acccttccaa gcgcctgacg ccctgtgatt tttcgtctcc gcgtcagaat cgtcctgcac
      181 gacggcacaa ctgcatctgt cgtatgtgtg ccggccgata cacgagacgg gccgtagcgc
      241 aatcttcacc atcccttgcg aatcggttag tcttttggcg ttcggctgcg cagcgcacag
      301 ggaaccggtg tcccacgctc ggccgcagcc gctatctggc ttgtccggtt ccaaccccct
      361 catcgaggaa aaagacaatg aagactgcga tcgtgaagac gctgaccgtg gccgccgctg
      421 ccatcatggt tgccaccgcc accggctgca ccagcaagga cacgctggcg cgcatcgacg
      481 ccgccaacgc tgctgccaag caggccgcgt cggacgccgc tgccgccaag cagtcggctg
      541 atgccgcggc tgctgccgcc tcgagcgcgc agaacgccgc ttcgagcgca cagagcacgg
      601 cgaaccaggc gctgcaggcc tcccaggcct cgcagtcgtg ctgcgacgcg accaacgaga
      661 agatcgatcg cgccttcaag cgctcgatgg gcaagtaacc cgcccgtcag gtcgctgagc
      721 gacacgaaaa cgccgcggac ttccgcggcg tttttgtttg cggcgcccgc gagtccgccg
      781 ggcgcagcgg tccgtatgct ccgtccgtca gcgcgtcgcg ccgcgctcgc cttcgacgtg
      841 ccgggcgacc tcggtgggct cggcggctgg cgccggcggg tcggtcacgg ggcgcgcgta
      901 gtcacgcatg ccgatgcgca ccggcacccc ggacatctgc tggaaggcct gccgagccgt
      961 gacccagtcg acctgcaccg gcgttcccgc cgtcgcgctc gccaccacgc gggcgagctc
     1021 ggccggatcg cggtgcgtcg gatcctgcgt gccttcgagc ggcgcctgcc tctcgacgta
     1081 gagcgtcccg cgcgaccagc cgaccttcgt cgtctgatcg atcaggttca ccttcgtgct
     1141 gaccggcacc atcgtgaaga actcggcgat gtcctccggg aacatgcgga tgcagccgtg
     1201 cgtgacctgc atgcccacgc ccgccgggcg gttggtgccg tggatcaggt aagcaccgct
     1261 cggaatggcg aggcgcatcg cgtattcgcc cagcggattg tccggtcccg gcggcacgac
     1321 cttcggcaac gggtcgccct tggcgatgtg ctcgaggcgc acggattcgg gtggattcca
     1381 gttgggccgc tcctgcttgg acgcgacctt cgtgacgccg agtggcgtct tccagtccat
     1441 cttgccggtg ctgatcgggt acgtgcgcac gacgcgtggc tggcccggct gcgcgggcgg
     1501 aaagtagtag agccgatgct ccggcaggtt gacgacgatg ccttcacgtg gtgcatcggg
     1561 caggatgaat cgcccgggga tcaccacctc ggtgccggcg cccggcagcc acggatcgat
     1621 gccggggttt gccgcgatga tttcctcgta gcccacgccg tagcgccggc cgatgtcgag
     1681 cagcgtatcc tcgtgttcgg ccacggccgt cgtgacgacc ccgatcacgt tgtcaccatt
     1741 ggtgggcagc aggtactcgg ccgcgcccgc gccggcctcg cccgcgacga gcggtgcgac
     1801 cagtgcgacc aggacgggaa ccagacccct gctgccgttg ctacggtgca tgcaaccttt
     1861 ccttgcggtg gagcatccat tgtaccgaca tggaatcgcg cggttcgttt cccggcacgc
     1921 ccaggtcctg cgttgacccc gggacggttc ggcccgtagg cttgccgccc caagggagac
     1981 ccatgggtta taaactgacg atcgagttgt cggaccgcga cctgcgccat ttccgacgcg
     2041 aattgcacaa ggcccgtgag tcggtccaga tcgccgacga cgaggaaatc ctcggcgcag
     2101 cggccgacct cgtcgcggcc atgcggcgca ccgacctgcc ggacttcgtc accgagcggc
     2161 tcgaaaagct cgaaacgctg ctcgccatgg tcatggacca ccagtggcct ctcaccgacg
     2221 aggagcgcag cccggtgctg tcgggcctcg cgtacctctg cgatcccgag gacctgattc
     2281 cggacgacat cccgggcatc ggcatgctcg acgacgccgt catgatcgaa ctcgtgttcc
     2341 gcgaactgcg gcacgagctc gacgcctacg ccgacttcct gaagtttcgc gagcagctgg
     2401 tcaagggagg tggccggccg ccgacgccgc cggtcgaggc gaagctcgaa cgccgtcgcc
     2461 ggcagctgct ggagaggatg cgccgtcgca ggaaagtggc tagtggctag tggtcagtgg
     2521 ctagtgcaag acagcgaagg ccaacgtaac gctgaccctc tgcctcttca ctagccacta
     2581 gccactaacc actaaccact gatcactttc cccagcaact gctcgatctg ccccggcacg
     2641 ccgttgcgtg cctcggtgat cgcgacgcgc acggcgttcg cgaacgacac gcggtcggcg
     2701 ccgccatggc tcttgatgac gacgccgttc agcccgacca tgctcgcgcc gttgtagccg
     2761 cgcgggtcca ggcggccctt gagcgcgttc agcgcgggcg tcgcggccag cgcgccgagc
     2821 ttgcgcagcg ggtcgcgcgt gaattcctct ttcatgacgc cgctgatgaa gcgggccagg
     2881 ccttccatcg tcttgagcga gacgttgccg ctgaagccgt cggtcacgac cacgtcgacg
     2941 cccccggaaa agatgtcgtc gccctcgacg aacccgcagt aattgagcgc ggccgtcgac
     3001 tgcaggcgct tgtgcgcctc cttgatctct tcggtgccct tgatgtcttc ctcgccgacg
     3061 ttgagcagcc cgacgcgcgg gttggcgacc ccgtgcaggt ccgcggcgat gaccgagccc
     3121 atcactgcga actgcacgag gttcccggcg gtgcaatccg gattggcgcc gagatcgagc
     3181 atcacggtgt ggccgtgcac ggccggcacg gcagagacga tcgcgggtcg gtcgatgccg
     3241 ggaatcatgc cgagcacgaa ccgcgaagtg gcgagcagcg cgcccgtgtt gccggccgac
     3301 acgcacgcgg cggcctcacc ctgctcgacg aggttgatcg cgacccgcat cgacgacttc
     3361 ttcttccggc gcagcgcgtc ctgcggacgc tcgtccatgc cgacgacctc ggtcgcctcg
     3421 acgaaccggc aacgctcggt ctcgccgcgc cgccccagca acggtcgcac gacctcgccg
     3481 cggccgacga tgatcagccg caggtccgcg gtcacggcga ggacttcgag cgcggcggga
     3541 accgccacct cggcgccgtg gtcgccgctc atcgcgtcca cggcgactgt caactgcccg
     3601 gcgttcatgc gcgggcgcgg gtggtcagga attactcctg gccttcgtcg gtcgccggct
     3661 tctcgatcac cttcttgccg cggtagaagc cgtccggcgt gatgcggtgc cgagcgtgcg
     3721 tttcgccgct cgtgcgatcg accgacagcg ccgggccgcg caggtggtcg tgcgaacgat
     3781 gcatgccgcg cttcgacggg gtcttgcggc tcttctggac tgccatgatc acttctcctg
     3841 atgcgggtca gcgctcgttc ttcttctcga gcaattcccg cagattcgca aagggcgtct
     3901 gcttctcttc cgccgctgca ccggcagccg gcggatcggc ggcaagcggc cgcaccggcg
     3961 cgcttcccgc tccatcaatg ttcgcgaggc actgcggatc gtccgccgcg tgcatcggca
     4021 cgagcggcag cgccagcaac acctgctcct cgaccagcgc cgcgagcgac aactgctcgg
     4081 gcaaccccac gaacggctcg aacccgccgg ggacgtcctc gtcctcgtcg cgcaccacca
     4141 tgacctgcgc ggactcgtcg acgtgcgtcg tgcagggctg caggcagcgc tggcacgcca
     4201 gcgttgccac accgtcgacc agcacggcca ccgtcgttcg gccgtcgaaa gcgccgaacc
     4261 gcagctgcgc atcgatgcga gtgccgggta gcccgcccgc gtcccgcacg cggtcgaggt
     4321 gcgacagctc cagcctgcgc tcgacgactg cggctcgcgc cgacagctcc ttagcgttga
     4381 cctgatcggc cgggccgggc gacatgggcc gcgtataata gtgagcgccc ctgcccctgt
     4441 caaaaataag acgagcctaa atcgttgttt tccaagggac tgattctcgc gtctacctcc
     4501 cggtatcgac gttcgctgct cggccgcctc ggcctgccct tcgactgcgt cgcgcccgaa
     4561 gtggacgaga cgcgccagcc cgacgagtcc gcggtcggca tggccgcgcg gctggcccgg
     4621 ctcaaggcgg aggcggtgtc gaggttgcac ccgtccgctg tcgtgatcgg ctccgaccag
     4681 gtcgcgctgc gcggccacga cctgctcggc aagcctggca cggtggagca ctgtcgggag
     4741 cagctgcagc ggtcgtcggg ccgcgacgtc gtgttcctca ccgcggtgca cgtgatcgac
     4801 ggcccgcaag gacgtgcgga gtcgcacgtc gaccgcacga cggtgctgtt ccgcgagttg
     4861 tcggccaccg agatcgatcg ctacatcgag cgtgaccggc cgctcgactg cgcaggcgga
     4921 ttcaaggcgg agtccctcgg catcgcgtt
//
LOCUS       DASPFR010000348         4338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_713564, whole genome shotgun sequence.
ACCESSION   DASPFR010000348 DASPFR010000000
VERSION     DASPFR010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713564"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VD737_09210"
     CDS             complement(<1..829)
                     /locus_tag="VD737_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020752349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HXW10782.1"
                     /translation="MATIREDDFIQSVADALQFISYYHPVDYIEHLGRAYEREQSPAA
                     KDAIAQILTNSRMCAEGRRPICQDTGIVNMFLKVGMGVRWDTRRSLGDMIDEGVRRAY
                     GHPDNTLRASVVDDPLFARKNTQDNSPAVFHVELVPGEEVEVTVAAKGGGSENKSKFV
                     MLNPSDSIVDWVLKTVPTMGAGWCPPGMLGLGVGGTAEKAMLLAKEALMDPIDMHELL
                     ARGPATKAEELRIELYEKVNALGIGAQGLGGLTTVLDVKLKLYPTHAASKPVAMIPNC
                     "
     gene            complement(891..2327)
                     /gene="gatB"
                     /locus_tag="VD737_09215"
     CDS             complement(891..2327)
                     /gene="gatB"
                     /locus_tag="VD737_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020297121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HXW10783.1"
                     /translation="MNWETVIGLEIHAQLATRSKIFSGSSTAFGAEPNTQANLVDLGY
                     PGVLPVLNREAVRMAVRFGLATGAKISRRSVFARKNYFYPDLPKGYQISQYERPVVED
                     GSLDVMLDDGSRKTIGITRAHLEEDAGKSLHEDFHGLSGIDLNRAGTPLLEIVSEPDL
                     RSAREAVAYMKKIHTLVRYLDICDGNMQEGSFRCDANVSVRPVGQAEFGTRAEIKNLN
                     SFRFVERAINYEVERQIELLENGGKVVQETRLYDPDKGETRSMRSKEEANDYRYFPDP
                     DLLPLAIDEATIDEVRASMPELPDEKAARFVREHGLSDYDAGVLTASKELAQYYESVV
                     ARLGSQPKLAANWVMGELSGALNRDNLEVTQSRVDAGALAGLLTRIVDSTISGKIAKD
                     VFEAMWTDGLEADAIIDAKGLRQITDDAAIERAIDEVMARNPGQLAEYRAGKDKLFGF
                     FVGQVMKATGGKANPGQLNELLKRKLSS"
     gene            complement(2439..3905)
                     /gene="gatA"
                     /locus_tag="VD737_09220"
     CDS             complement(2439..3905)
                     /gene="gatA"
                     /locus_tag="VD737_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HXW10784.1"
                     /translation="MHTLTVSELSAGLRARRFSSLDLTLHLLGRIERLGPELNAFVTI
                     TADRALADARAADEKLARGEGGPLTGVPVAHKDIFCTDGVLTTCASRMLSNFVAPYDA
                     TVVRKWREAGTVLLGKTNMDEFAMGSSNETSFYGPVRNPWDRTRVPGGSSGGSAAAVA
                     ACLAPAASGTDTGGSIRQPAALTNLTGLKPTYGRVSRYGMIAFASSLDQAGVLARSAE
                     DAALMLEAMAGFDPNDSTSVDTPVPPYAAELERPLKGLRVGLIREFFGEGLDPAVERC
                     VRESIALLQREGAVVKDVSLPSLPLSVPTYYVVAPAECSSNLSRFDGVRFGHRCEAPK
                     DLLDLYKRSRGEGFGEEVKRRIMTGTYVLSAGYYDAYYLKAQKCRALIARDFARAFEE
                     VDVVLSPTSPTAAFELGAKTSDPIAMYLNDIYSIAANLAGLPGISIPCGFIHDGGTSL
                     PVGLQLVGPHFGESRLLAVAHQYQVRTEWHKRVPPGYA"
     gene            complement(3992..4282)
                     /gene="gatC"
                     /locus_tag="VD737_09225"
     CDS             complement(3992..4282)
                     /gene="gatC"
                     /locus_tag="VD737_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004574467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HXW10785.1"
                     /translation="MSLTRAQVEGIAHLARLEITAAQMPAYVDSLSRIIDFVEQLSSA
                     DTADVEPMAHPLADQVQRLRPDEATETDRRDKFQHNAPSVAAGLYLVPKVIE"
BASE COUNT          656 a         1436 c         1474 g          772 t
ORIGIN      
        1 cgcagttggg gatcattgcg acgggcttcg acgccgcgtg ggtcggatac agcttcagct
       61 tgacgtcgag caccgtcgtg agcccgccga ggccctgcgc gccgatgccg agcgcgttga
      121 ccttctcgta cagctcgatg cgcagctctt cggccttggt ggccgggccc cgcgcgagga
      181 gctcgtgcat gtcgatcggg tccatcagcg cttccttcgc cagcagcatg gccttctcgg
      241 ccgtgccgcc cacgcccagc ccaagcatgc cgggcgggca ccagccggcg cccatggtcg
      301 gcacggtctt cagcacccag tcgacgatcg agtcgctcgg gttgagcatc acgaacttgg
      361 acttgttctc cgacccgccg cccttggcgg cgacggtgac ttcgacctcc tcccccggca
      421 cgagctcgac gtggaacacg gcgggcgagt tgtcctgcgt attcttgcgc gcgaacagcg
      481 ggtcatcgac caccgaggcg cgcaaggtgt tgtcgggatg cccgtacgcg cgccgcacgc
      541 cttcgtcgat catgtcgccg aggctgcgcc gcgtgtccca gcgcacgccc atgccgacct
      601 tgaggaacat gttgacgatg ccggtgtcct ggcagatcgg gcgccggccc tccgcgcaca
      661 tgcgcgagtt cgtcaggatc tgcgcaatcg catccttcgc ggccgggctc tgctcgcgct
      721 cgtacgcgcg gcccaggtgc tcgatgtagt cgaccgggtg gtagtacgag atgaactgca
      781 gcgcgtcggc gacgctctgg atgaagtcgt cttcgcggat ggtggccatg gcgaaacccg
      841 tgtctggtgt ctagcggttg gtaggaaagc gctggcgcgc ctcctgcaga tcagctggag
      901 agcttgcgct tgagcagctc attcaactgg ccgggattgg ccttgcctcc cgtggctttc
      961 atcacctgcc ctacgaagaa accgaacagc ttgtccttgc ccgcacggta ctcggcgagc
     1021 tggccggggt tcctcgccat gacctcgtcg atcgcgcgct cgatcgccgc gtcgtcggtg
     1081 atctgcctga ggcccttcgc gtcgatgatc gcgtcggcct cgaggccatc cgtccacatc
     1141 gcctcgaaca cgtccttggc gatcttgccg gagatcgtgc tgtcgacgat gcgcgtcagc
     1201 agcccggcca gcgcgccggc gtcgacgcgg ctctgcgtca cctcgaggtt gtccctgttg
     1261 agcgcgcccg aaagctcgcc catgacccag ttggccgcga gcttcggctg gctgcccagc
     1321 ctggcgacga cgctctcgta gtactgcgcg agctccttgc tggccgtgag cacgccggcg
     1381 tcgtagtccg aaagcccgtg ctcgcgcacg aaccgggcgg ccttctcgtc cggcagttcg
     1441 ggcatcgacg cacgcacctc gtcgatggtc gcctcgtcga tcgcgagcgg cagcaggtcc
     1501 gggtccggga agtagcgata gtcgttggct tcttccttcg agcgcatcga gcgcgtctcc
     1561 cccttgtcgg gatcgtagag gcgcgtctcc tggacgacct tgccgccgtt ctcgagcaac
     1621 tcgatctgcc gctcgacctc gtagttgatc gcgcgctcga cgaagcggaa cgagttcagg
     1681 ttcttgatct cggcgcgggt gccgaattcc gcctggccca cgggccgcac cgacacgttg
     1741 gcgtcgcagc gaaacgagcc ctcctgcatg ttgccgtcgc agatgtcgag gtagcgcacg
     1801 agcgtatgga tcttcttcat gtatgccact gcctcccggg ccgagcgcag gtcgggctcc
     1861 gacacgatct cgagcagcgg cgttccggcg cggttcaggt cgatgcccga caggccgtgg
     1921 aagtcctcgt gcagggactt gccggcgtcc tcctcgagat gcgcgcgcgt gatcccgatg
     1981 gtcttgcggc tgccgtcgtc gagcatgacg tcgagcgaac catcctcgac gaccggccgc
     2041 tcgtactggc tgatctggta gcccttcggg aggtcgggat agaagtagtt cttgcgtgcg
     2101 aagaccgagc gacgcgagat cttcgccccc gtcgccaggc cgaatcgcac ggccatacgc
     2161 accgcctccc ggttcagcac gggcagcacg cccgggtagc ccaggtcgac gaggttggcc
     2221 tgcgtgttcg gctcggcgcc gaatgccgtg gacgagccgg aaaagatctt gctgcgcgtg
     2281 gcgagctgcg cgtggatctc gaggccgatg acggtttccc agttcatagg acagtggtca
     2341 gtgattagtg gctagtggtc agtgaggagg ttgaggttgc ggcttcgcct gggtagcggt
     2401 tcatactagc caccagccac tagccaccag ccacttcctc atgcgtagcc cggcggcacg
     2461 cgcttgtgcc actcggtccg cacctggtac tggtgcgcca ccgcgagcag ccgcgattcg
     2521 ccgaagtgcg ggccgacgag ctgcaggccg acggggagtg atgtgccgcc gtcgtggatg
     2581 aagccgcacg ggatcgagat cccgggcagg cccgcgaggt tcgcggcgat cgagtagatg
     2641 tcgttcaggt acatcgcgat cgggtcgctg gtcttggcgc cgagctcgaa cgccgcggtc
     2701 ggcgacgtcg ggctcagcac gacgtccact tcctcgaacg cgcgggcgaa atcgcgggcg
     2761 atcaaggcgc ggcacttctg cgccttcagg tagtacgcgt cgtagtagcc ggccgagagc
     2821 acgtacgtgc cggtcatgat gcggcgcttg acctcctcgc cgaagccctc gccccgcgag
     2881 cgcttgtaga ggtcgagcag gtccttcggt gcttcgcagc ggtgaccgaa gcgcacgccg
     2941 tcgaatcgcg agaggttcga ggagcactcg gcgggcgcaa ccacgtagta cgtggggacg
     3001 gacagcggca ggctcggcag gctcacgtcc ttgacgacgg caccttcacg ctgcagcagc
     3061 gcgatggact cgcgcacgca gcgctcgacc gcgggatcga gcccttcgcc aaagaactcg
     3121 cggatcaggc caacgcgcag tcctttgagc gggcgctcga gctcggctgc gtagggcggt
     3181 accggcgtgt cgacgctggt ggagtcgttg gggtcgaacc cggccatcgc ttcgagcatc
     3241 agcgcggcgt cctcggccga gcgcgcgagc acgccggcct ggtccaggct cgacgcgaac
     3301 gcgatcattc cataccgcga cacacgcccg taggtcggct tgagcccggt caggttggtg
     3361 agcgccgcgg gttggcggat cgagccgccg gtgtcggtac cgctcgcggc cggagcgaga
     3421 cacgcggcga ccgccgccgc agaaccgccc gacgagccgc ccggcacgcg cgtgcggtcc
     3481 catggattgc gtacgggccc gtagaacgat gtttcgttgg acgagcccat cgcgaactcg
     3541 tccatgttcg tcttgccgag cagcaccgtg cccgcctcgc gccacttgcg gaccaccgtc
     3601 gcgtcgtacg gtgcgacgaa gttcgagagc atccgcgacg cgcaggtcgt gagcacgccg
     3661 tccgtgcaga agatgtcctt gtgcgcgacc ggcacgccgg tcaacggccc gccctcgccg
     3721 cgggcgagct tctcgtcggc ggcgcgcgcg tccgccagcg cgcggtccgc ggtgatggtc
     3781 acgaacgcgt tgagttcggg cccgagccgc tcgatgcggc cgagcaggtg cagcgtgagg
     3841 tcgaggctcg agaacctgcg cgcgcggagg ccggccgaga gttccgatac ggtcagtgtg
     3901 tgcattcggg ggactgtccc cagtgttccg ggagaaaaat ggggactgtc cccatttcga
     3961 cgttcatgac cgcaccctta cggatgcatc ttcactcgat caccttcggc acgagatata
     4021 gtcccgcagc gacgctcggc gcgttgtgct ggaacttgtc gcggcgatcg gtctcggtgg
     4081 cctcgtcggg ccgcagtcgc tgcacctggt cggcgagcgg gtgcgccatc ggctcgacgt
     4141 cggcggtgtc cgcggacgac agttgctcga cgaagtcgat gatgcgcgac aggctgtcga
     4201 catacgcggg catctgcgcc gcggtgatct cgagacgtgc gaggtgggcg attccctcga
     4261 cctgggcacg ggtcaggctc atctgaaaaa gctcgatcac gccgcaaaaa gacggtcgca
     4321 aatgatacac ggcgcctt
//
LOCUS       DASPFR010000349         8003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1446330, whole genome shotgun sequence.
ACCESSION   DASPFR010000349 DASPFR010000000
VERSION     DASPFR010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8003
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446330"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /gene="rho"
                     /locus_tag="VD737_09230"
     CDS             complement(<1..954)
                     /gene="rho"
                     /locus_tag="VD737_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HXW10786.1"
                     /translation="MNLSELKHKPIPKLLELAQTMGLENLARSRKQDIIFSILKAHAR
                     NGESIYGDGVLEILQDGFGFLRSPEGSYLAGPDDIYISPSQIRRFNLRTGDTISGLIR
                     PPKDGERYFALLKVGEINFDSPDNARSKVLFENLTPLHPTRRFKLERGTGSTEDLTAR
                     MIDLIAPLGKGQRGLIVSPPKAGKTMLLQNIANSITGNHPEVYLIVLLIDERPEEVTE
                     MTRTVRGEVVSSTFDEPASRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNT
                     VVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTILAT"
     gene            complement(1128..1454)
                     /gene="trxA"
                     /locus_tag="VD737_09235"
     CDS             complement(1128..1454)
                     /gene="trxA"
                     /locus_tag="VD737_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008073352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HXW10787.1"
                     /translation="MSQQIVHVSDSSFEQDVLKSSVPVLLDFWAEWCGPCKMIAPILD
                     QIAAEYAGKVLVAKMNVDESPRTPTKYSVRSIPTLMLFKNGTVIDQKIGAVRKADVAA
                     FLDNSL"
     gene            1763..3316
                     /locus_tag="VD737_09240"
     CDS             1763..3316
                     /locus_tag="VD737_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXW10788.1"
                     /translation="MSDTHLTDVSFTQLHLPEALARGIADAGFERCTPIQAQTLPRAL
                     AGFDIAGQAQTGTGKTAAFLVAMYSKLLREDAPAGRQVNHPRALIIAPTRELAVQIHK
                     DAEILGQYTGLTHGLAFGGVDYEKQRRHLEQGVDVLIGTPGRLIDFYKQRVFDLRSIQ
                     VVILDEADRMFDLGFIADIRYIMRRLPPPDQRLNLLFSATLAQRVLELAFEHMNDPEL
                     VRIEPDKMTVDRVRQLIYYPSMDEKPRLLIGLLRQMDPHRTMVFVNTRRGADELEGLL
                     KANGLNAEAISGDVPQSKRLRMIRDFLSGDLAILIGTDVASRGLHIPDVSHVFNYDLP
                     QDPPDYVHRIGRTARAGAEGDAISFGCEDFVQSLPDIEDYIGRRIPVAAVAQELLAEI
                     TVPRHERRRHAGPGAGSRHGGGGRSRDGGRSTSSRGSRERPRGPRRETRPPPSAAPTP
                     RPSPAPRPEADAVEAARAPATDNAAGNGADGEKKKRRRRRGGRGRSGQGQGPERTTPP
                     SGTDTASES"
     gene            complement(3259..4011)
                     /gene="yrfG"
                     /locus_tag="VD737_09245"
     CDS             complement(3259..4011)
                     /gene="yrfG"
                     /locus_tag="VD737_09245"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF011564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HXW10789.1"
                     /translation="MTPDFVVRPTPAPDWSRIDTVCLDMDGTVLDLRFDNLFWLEELP
                     RRWGAARGLDRAAAMARLKPRFDAKRGTLDWYCVDHWSEELGLDVPALKHELRGEIRY
                     LDGAPEFLDLLRATAKRVLLTTNAHPISLRVKDAQTRLSRHFDELVSSHEFGAPKESP
                     QFWERLRVDHSVDLRRTLFVDDSHAVLHAALAAGVAWAYQVLQPDSTQPPHAPLADVP
                     GILRLADLAESLRAQLSDAVSVPDGGVVRSGP"
     gene            4081..4911
                     /gene="cysQ"
                     /locus_tag="VD737_09250"
     CDS             4081..4911
                     /gene="cysQ"
                     /locus_tag="VD737_09250"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HXW10790.1"
                     /translation="MNTAAFDAPAASDDLAALLPEIRKLVADAGTAILEVYAGAHGVE
                     YKSDDSPITRADRAAHEVLTAGLRRLTPAIPVLSEESADEHAVEVRRHWRELWLVDPL
                     DGTKEFIGRNGEFTVNVALVRDHRPVLGVVAAPVLDTTYYGVQGHGAFVARQGEPDRP
                     IRVRRPAAEPLVIVGSRSHRGDSLDSVLARLGPHELRPMGSSLKFCLVAEGAADLYPR
                     LGPTSEWDTAAAQAVVEAAGGAVTTLDGAPLRYNERDTLLNPYFLAFGDPSRDWHRLF
                     "
     gene            4918..5232
                     /locus_tag="VD737_09255"
     CDS             4918..5232
                     /locus_tag="VD737_09255"
                     /inference="COORDINATES: protein motif:HMM:NF023171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HXW10791.1"
                     /translation="MQLSWGLLLLVLAMGGFALFWHDTLGARDRANAAARETCEATGA
                     ALLDGTVSFHRLRVVRGDDGRPALERTYLFDYSTDGVTRRQGFVVVAGRRVESVGLQP
                     LA"
     gene            5300..5524
                     /locus_tag="VD737_09260"
     CDS             5300..5524
                     /locus_tag="VD737_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HXW10792.1"
                     /translation="MSENKDNVDTETFQHLQRLRSLRIEHRDLDDVIHRLQLDLYVDQ
                     VQLRRLKKRKLLLKDQIARLESQLIPDLNA"
     gene            5532..6863
                     /locus_tag="VD737_09265"
     CDS             5532..6863
                     /locus_tag="VD737_09265"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HXW10793.1"
                     /translation="MPKPLQVISEVLTTLQVPENALQVALIVAALGLGWWAARYVRSR
                     ITVSPDPDNIPDRLRELLYVGAPQSLVLAVLATLDGLMHAFGLEAGMLDVAVQLVGVL
                     LLIRVTVYVIRVSLGARTRLKGWGVPITAVVWVFLALHVLGWGDEVIAALDSIGMNAG
                     KTRISVWSVMKLLVTVSVFVVIAMWASRWLERRVLKLDALAPTMRIGIAKFLQAFLVG
                     LSVLIGLNAAGLDLTTLNVLTGAIGIGLGFGLQSIAANFVSGFVLLMDRSIKPGDVIS
                     FTGMPGTTTEGFGWVEELRGRYVVVRDRDGVETLVPNQNLITNPVINWSYTDPRVRLK
                     LPVRLSYKDDPELAMRLLLEATDGHPRIIREPAPVARMMGFGDHGIELELRFWIPDPQ
                     EGVNNVRSDVNRRIWALFREHGLTIPVAQREIRVEMGPTQVPAPPSLPRGD"
     gene            6867..7286
                     /gene="arfB"
                     /locus_tag="VD737_09270"
     CDS             6867..7286
                     /gene="arfB"
                     /locus_tag="VD737_09270"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HXW10794.1"
                     /translation="MPDEALPAPSIPDELLEFRAVRASGPGGQNVNKVSNAVELRFDS
                     ARWDALSPGARSRLRRIAGRRMTAAGVIVIDAQRLRSLEQNRADALERLQAMIEEALV
                     EPKPRRKTRPTLASKRRRLEGKVQRGKVKALRRKPED"
     gene            complement(7531..>8003)
                     /locus_tag="VD737_09275"
     CDS             complement(7531..>8003)
                     /locus_tag="VD737_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10795.1"
                     /translation="AIVFDARPRLAEPAFERDEATVVLDAEGDVVVAGDPGEPPNGVR
                     VLGVEAPARRLRLADGPPLRYDSLNARLPLPRLVELLGRYAPRGVREAAATLRTLASS
                     TGDAGDAGAAADFSRNVVRIHHWLATVDIRPSMPAADHAATEAERTLAARTSAL"
BASE COUNT         1229 a         2761 c         2758 g         1255 t
ORIGIN      
        1 cgtggcgagg atcgtgagcg agccgccctc ctcgatgttg cgcgcggcgc cgaagaagcg
       61 cttcggccgc tgcaatgcgt tcgcgtcgac gccgccggtc agcaccttgc cggaactcgg
      121 caccacggtg ttgtaggcgc gcgccaggcg cgtgatcgag tcgagcagga tcacgacgtc
      181 cttcttgtgc tcgaccaggc gcttggcctt ctcgatcacc atctcggcca cctgcacgtg
      241 acggctcgcc ggctcgtcga acgtcgagga caccacttcg ccgcgcaccg tgcgcgtcat
      301 ctcggtgact tcctcgggcc gctcgtcgat cagcagcacg atcaggtaca cctcggggtg
      361 gttgccggtg atcgagttgg cgatgttctg cagcagcatg gtcttgccgg ccttcggcgg
      421 cgagacgatc aggccgcgct ggcccttgcc gagcggcgcg atcaaatcga tcatgcgcgc
      481 ggtgaggtcc tccgtgctgc ccgtgccgcg ctcgagcttg aagcgccggg tcgggtgcag
      541 cggcgtcagg ttctcgaaca ggaccttgct gcgcgcgttg tcgggcgagt cgaagttgat
      601 ctcgccgacc ttgagcagcg cgaagtagcg ctcgccgtcc tttggcggac ggatcaggcc
      661 ggagatcgtg tcgccggtgc gcaggttgaa gcggcggatc tgcgaggggc tgatgtagat
      721 gtcgtccggg cccgcgaggt acgagccctc gggcgagcgc aggaagccga acccgtcctg
      781 caggatctcg agcacgccgt cgccgtagat gctctcgccg ttgcgggcgt gcgccttcag
      841 gatcgagaag atgatgtcct gcttgcgcga gcgggccagg ttctcgaggc ccatcgtctg
      901 ggcgagttcg agcagcttcg ggatcggctt gtgcttgagc tcgctcaggt tcatcgagcg
      961 gcggctggca tcgagctcgg ccgggctgat gacgtcgagg tcgtcgccgt cgagaaaatc
     1021 gtcctgcggc aactcatcct gtcgggggcc gcgattgccg tccgggccgc cgcctccgcg
     1081 gccgcgcttg cccgggcggt tgcgaccctg gttgccgagg tatcccctca caggctgttg
     1141 tccagaaaag ccgcgacgtc ggccttgcgg accgcgccga tcttctgatc gattactgtg
     1201 ccgttcttga aaagcatcag agttgggatg cttctgacgc tgtacttcgt tggggttctg
     1261 ggactctcgt ccacgttcat cttcgcgacg agcaccttcc ccgcgtactc cgccgctatc
     1321 tggtccagta tcggtgcgat catcttgcat ggtccgcacc actcagccca gaagtccaga
     1381 aggacgggaa cgctcgactt gagcacgtcc tgctcgaagc tcgagtctga gacgtggacg
     1441 atctgctggc tcaccagaga ctacctccgc tggcgtaagg aaatgattgg gcacgggtgt
     1501 tttcttgggt ccgaaggggt tggcgtcacc gggaatcggg atgacggcgg gttccgcgta
     1561 gtgcaaacta tcgggattgc tggacttgga tcgggttcga aaggaattcc gtagctggcg
     1621 ccggttcgcc gggcgggagg gaccggactt aaggcccgat cagccggcga aatcatctgg
     1681 cgggagcgga tggatccgcg attccggata tttgaacccg cttttccgca ttttgcaagg
     1741 ccgctccggc cgatcccgac gaatgtcaga cacacacctc accgacgttt ccttcaccca
     1801 actccacctg cccgaggcgc tcgcccgcgg catcgccgac gccggcttcg agcgctgcac
     1861 cccgatccag gcgcagacgc tgccgcgcgc gctggccggg ttcgatatcg caggacaggc
     1921 ccagaccggc accggcaaga ccgcggcctt cctggtcgcc atgtactcga agctgctgcg
     1981 cgaggatgcg ccggcgggcc ggcaggtgaa ccacccgcgc gcgctgatca tcgcccccac
     2041 ccgcgaactc gcggtgcaga tccacaagga cgcggagatc ctcggccagt acacgggcct
     2101 cacgcacggg ctcgccttcg gcggggtcga ctacgagaag cagcgtcgcc acctcgagca
     2161 gggcgtcgac gtgctgatcg gcacgcccgg acgcctgatc gacttctaca agcagcgcgt
     2221 gttcgacctg cgctcgatcc aggtggtgat cctcgacgag gccgatcgca tgttcgacct
     2281 cggcttcatc gccgacatcc gctacatcat gcggcggctg cccccgccgg accagcggct
     2341 gaacctgctg ttctcggcga cgctcgcgca gcgcgtgctc gagctcgcgt tcgagcacat
     2401 gaacgacccg gagctcgtcc gcatcgagcc cgacaagatg accgtcgacc gcgtccggca
     2461 gctgatctac tacccgtcga tggacgagaa gccgcgcctg ctgatcgggc tgctgcgcca
     2521 gatggacccg caccgcacga tggtgttcgt caacacgcgg cgcggcgccg acgaactcga
     2581 gggcctgctc aaggccaacg gcctcaacgc cgaggcgatc tcgggcgacg ttccgcagag
     2641 caagcgcctg cgcatgatcc gcgacttcct gagcggcgac ctggcgatcc tgatcggcac
     2701 cgacgtcgcc tcgcgcgggc tgcacatccc cgacgtgagc cacgtgttca actacgacct
     2761 gccgcaggat ccccccgatt acgtgcaccg gatcggtcgc acggcgcgcg cgggcgcgga
     2821 gggcgacgcg atcagcttcg gctgcgagga cttcgtgcag tcgctgccgg acatcgagga
     2881 ctacatcggg cgacgcatcc cggtcgcggc ggtcgcgcag gaattgctcg cggagatcac
     2941 ggtgccgcga cacgagcggc gccgtcacgc cgggcccggc gccggttcgc gccacggcgg
     3001 cggtggtcga tcgcgcgacg gcggacgcag caccagcagc cgcgggtcgc gcgagcgccc
     3061 gcgtggcccg cgccgcgaaa ccaggccgcc gccctccgcg gcgccgacgc cccgcccgtc
     3121 accagccccg cggcccgagg ccgacgccgt tgaggctgcc cgggcgcccg cgacggacaa
     3181 cgcagccggc aacggcgcgg acggcgagaa gaagaagcgg cgccggcggc gtggcggacg
     3241 cggacgctcc ggccagggtc aggggcccga acggacgacg cccccgtccg gaaccgacac
     3301 cgcgtccgag agctgagcgc gcaacgactc cgcgaggtcc gccagccgga ggatccccgg
     3361 cacgtcggcg agcggagcgt gcggcggctg cgtcgagtcc ggctgcagca cctggtaagc
     3421 ccaggccacc ccggcggcga gcgccgcatg cagcaccgcg tggctgtcgt cgacgaacag
     3481 cgtgcggcgc aggtcgacgg agtggtcgac gcgcaggcgc tcccagaact gcggcgactc
     3541 cttcggcgcc ccgaattcgt gcgacgacac cagttcgtcg aagtgacgtg acagtcgggt
     3601 ctgcgcgtcc ttgacgcgca ggctgatcgg gtgggcgttg gtcgtcagca gcacgcgctt
     3661 ggccgtcgcg cgcaacaggt ccaggaactc gggagcgccg tccaggtaac ggatctcccc
     3721 gcgcaactcg tgcttgagcg cgggcacgtc caggcccagt tcctcgctcc agtgatcgac
     3781 gcagtaccag tccagcgtgc cgcgcttcgc gtcgaagcgc ggtttcagcc gggccatcgc
     3841 cgcggcgcga tcgaggccgc gcgccgcgcc ccagcgacgc ggcagttcct cgagccagaa
     3901 caggttgtcg aagcgcaggt cgagcaccgt gccgtccatg tcgaggcaga cggtgtcgat
     3961 ccggctccag tccggcgcgg gcgtaggacg aacgacgaag tccggggtca tgcaggctcg
     4021 ggtgacggca gtcggcgggg agcgccgcta tcatacggtg cagacatcga ggagatccgc
     4081 ttgaacactg ccgccttcga cgcacctgcc gcttccgacg accttgccgc gctgctgccc
     4141 gagatccgca agctggtcgc cgacgccggc accgcgatcc tcgaggtcta cgccggcgcg
     4201 cacggcgtcg agtacaagtc cgacgattcg ccgatcacgc gggcggaccg tgcggcgcac
     4261 gaagtcctca cggcggggct gcggcgcctc acgcccgcga tcccggtgct gtccgaggaa
     4321 tccgccgatg agcacgccgt cgaggtgcgc cgccactggc gagaactctg gctggtcgac
     4381 ccgctcgacg gcaccaagga attcatcggc cgcaacggcg agttcaccgt caacgtggcg
     4441 ctcgtgcggg accatcgacc ggtgctcggc gtggtcgcgg caccggtgct cgacacgacc
     4501 tactacggcg tgcagggcca cggcgcgttc gtcgcccggc agggcgagcc ggaccgtccg
     4561 atccgggtgc gtcggccggc cgccgaaccg ctcgtgatcg tcggcagccg gtcgcatcgc
     4621 ggcgactcgc tcgacagcgt gctcgcgcgc ctcggaccgc acgaactcag accgatgggc
     4681 agttcgctca agttctgcct ggtcgccgag ggcgccgccg atctctatcc gcgcctcggc
     4741 ccgacgtcgg aatgggacac ggccgcggcc caggcggtcg tcgaggccgc gggcggcgcg
     4801 gtgaccacgc tcgacggcgc gccgctgcgc tacaacgagc gcgacacgct gctgaacccg
     4861 tacttcctcg cgttcggcga cccgagccgc gactggcatc ggctgttctg acggccgatg
     4921 cagctgtcct gggggctgct gctgctcgtc ctcgcgatgg gcggctttgc gctgttctgg
     4981 cacgacacgc tcggagcccg cgaccgggcc aacgccgcgg ctcgcgagac ctgtgaggcc
     5041 accggcgcgg cactgctcga cgggaccgtg tcgttccacc ggctgcgggt cgtgcgcggc
     5101 gacgacggcc ggcccgcgct ggagcggacg tacctgttcg actactcgac cgacggcgtc
     5161 acgcggcgcc agggcttcgt cgtggttgca gggcgtcgcg tcgaaagcgt cgggctccag
     5221 ccgctggcct gagcctatcc ccttttgacc ggcggggagt aaagtacggg ctcccccgag
     5281 caccgtcgcg acgcatgcaa tgtcggaaaa caaggacaac gtcgacaccg agacgttcca
     5341 gcacctgcag cgcctgcgca gcctgcgcat cgagcaccgc gacctcgacg acgtgatcca
     5401 ccgcctgcag ctcgacctct acgtcgacca ggtgcagctg cgtcgcctca agaaacgcaa
     5461 gctgctgctg aaggaccaga tcgcccggct ggagagccag ctcatcccgg acctgaacgc
     5521 gtgacgtccg gatgccgaag ccgctgcagg tgatcagcga ggttctgacc accctgcagg
     5581 tgccggagaa cgcgctgcag gtcgcgctga tcgtcgccgc gctcgggctc gggtggtggg
     5641 ccgcgcgtta cgtccgttcg cgcatcaccg tctcgcccga tcccgacaac attcccgacc
     5701 ggttgcgcga actgctctac gtcggcgcgc cgcagtcgct ggtcctcgcc gtgctcgcga
     5761 cgctggacgg cctgatgcac gccttcgggc tggaggccgg catgctcgac gtggccgtgc
     5821 agctggtcgg cgtgctgctg ctgatccgcg tgaccgtcta cgtcatccgc gtgtcgctcg
     5881 gcgcgcgcac gcggctcaag ggctggggcg tgccgatcac cgccgtcgtc tgggtgttcc
     5941 tggcgctgca cgtgctgggc tggggcgacg aggtcatcgc cgcgctcgac agcatcggca
     6001 tgaacgccgg caagacgcgg atctcggtct ggtccgtcat gaagctgctg gtgacggtca
     6061 gcgtcttcgt cgtcatcgcg atgtgggcct cgcgctggct cgagcgacgc gtgctcaagc
     6121 tcgacgcgct cgcgccgacg atgcgcatcg gcatcgcgaa gttcctgcag gcgttcctgg
     6181 tcggcctgtc ggtgctgatc gggctcaacg ccgcgggcct ggacctcacg acgctcaacg
     6241 tgctcaccgg cgcgatcggc atcggactgg ggttcggcct gcagagcatc gcggccaact
     6301 tcgtcagcgg cttcgtgctg ctgatggacc gctcgatcaa gccgggcgac gtgatcagct
     6361 tcaccggcat gccgggcacg acgaccgagg gcttcggctg ggtcgaagag ctccgtggac
     6421 gctacgtcgt ggtgcgcgac cgcgacggcg tcgagacgct cgtgccgaac cagaacctga
     6481 tcaccaaccc ggtgatcaac tggagctaca cggatccgcg cgtgcgcctg aagctgccgg
     6541 tgcggctcag ctacaaggac gatcccgagc tcgcgatgag actgctgctc gaggccacgg
     6601 acggacaccc gcgcatcatt cgcgagccgg cgcccgtcgc gcggatgatg gggttcggcg
     6661 accacggcat cgagctcgag ctgcggttct ggatcccgga cccccaggaa ggcgtcaaca
     6721 acgtgcgctc cgacgtcaac cggcgcatct gggcgctgtt ccgcgagcac gggctgacga
     6781 ttccggtggc gcagcgggag atccgcgtcg agatgggccc gacacaggtg cccgcgccgc
     6841 cgtcgctgcc gcgcggcgac tagcgcatgc cggacgaagc tttgcccgcg ccgtcgattc
     6901 ccgacgagct gctcgagttc cgcgccgtgc gcgcctccgg ccccggtggc cagaacgtca
     6961 acaaggtttc gaatgccgtc gagctgcgct tcgactcggc gcgctgggac gccttgtcgc
     7021 ccggcgcgcg ttcgcggctg cgtcgcatcg cgggacggcg catgaccgcc gcgggcgtga
     7081 tcgtcatcga cgcgcagcgg ctgcgatcgc tcgagcagaa tcgcgcggat gcgctcgagc
     7141 gcctgcaggc catgatcgag gaagcgctgg tcgagccgaa gccgcgccgc aagacgcgcc
     7201 cgacgctcgc gtcgaaacgt cgacgtctgg aaggcaaggt gcagcgcggc aaggtgaagg
     7261 cactgcgacg caaacccgag gattagtggc tagtggccag tggtcagtgg ctagtggaaa
     7321 aacaaaaagg ccggcgttct cgccggcctt ctgcactgac cactggccac taaccactga
     7381 tcactttctt tatgtctcgc tgccggtcgt cgtcgtccgt ggcgacgacc ggtgcaagac
     7441 aaaggtcatc cgtgacccgt ggaaagcaga ctacgcaatg cgccgcggcg tcgtgctcgg
     7501 cgcaacgatc aatgcgtcgt aggacctcgc tcacagcgcg ctcgttcgcg cagccaaggt
     7561 gcgctccgct tcagtcgccg cgtgatcggc ggccggcatc gacgggcgaa tgtcgaccgt
     7621 cgcgagccag tggtggatgc gcacgacgtt gcgcgaaaaa tccgctgcgg ctccggcgtc
     7681 gcccgcatct ccagtcgacg atgcgagcgt gcgcaaggtc gcggcggcct cgcgcacgcc
     7741 tcgcggcgcg tagcggccga gcagttcgac caggcgaggc agcggaagcc gggcgttcag
     7801 cgagtcgtag cgcaggggcg ggccgtccgc gagccgcagc ctgcgcgccg gtgcctcgac
     7861 gccgagcacg cgcacgccgt tcgggggctc acctggatcg ccggctacga cgacgtcgcc
     7921 ctcggcgtcg agtacgaccg tcgcctcgtc gcgctcgaac gccggctccg cgagccgtgg
     7981 ccgcgcatcg aagacgatcg ctt
//
LOCUS       DASPFR010000350         4122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_593142, whole genome shotgun sequence.
ACCESSION   DASPFR010000350 DASPFR010000000
VERSION     DASPFR010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593142"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1559)
                     /gene="nuoN"
                     /locus_tag="VD737_09280"
     CDS             complement(111..1559)
                     /gene="nuoN"
                     /locus_tag="VD737_09280"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HXW10796.1"
                     /translation="MNPASITRLDLLLAAPEMFLLGAACVVLLVDLFLSDRSRWVTFA
                     LSLVALAGTSLVTTMFAFTERTVGWHGTYVADPLSTLLKVVAVAAVAVAFLYAYDYLR
                     VRRLLKGEYFVLGLFALLGIMVLVSANSLVTLYLGVELLALSQYAMVAFNRESGVAAE
                     SAIKYFVLGSIASGALLYGMSIIYGVTGSLELGVVSAVASQMPAGQIGLLFGLAFIVV
                     GVAFKFGAVPFHMWIPDVYHGAPTPVTLFLAAAPKLGSFALAFRLLAEGLGPIHDSGW
                     RDMITFVAVASIVLGNVVAIAQNNIKRMLAYSTISHVGFILFGILAGTSEGYESALYY
                     TIVYVLMSAGGFGVVILLSRAGFEADSLDDYRGLNARSPWFAAVMLMLMFSMAGVPPF
                     VGFWAKLAVIQSVLHVGSVWLAILAVAFSVIGAYYYLRVVKLMYFDEPVERAAIEGSG
                     AMRFVLSVNGLAVLVLGLFPGMLLALCARVIP"
     gene            complement(1563..3077)
                     /locus_tag="VD737_09285"
     CDS             complement(1563..3077)
                     /locus_tag="VD737_09285"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HXW10797.1"
                     /translation="MQADWPILSVLVWLPIVAGVLLLLVGDRNAPLARWIALAASLVT
                     FALGVILWNDFDPSVASMQFVELQPWIGAFNAWYSLGVDGISLPLIVLTAFITPLVVI
                     AGWTVIEKRPAQYLASFLILEGLMIGVFAATDALLFYVLWEAMLIPMFVIIGVWGGPR
                     RVYATVKFFLYTFLGSVLMLVALIYLYLKGGSYSIADLQALPLGLREQVLIFVAFFLA
                     FAVKVPMWPVHTWLPDAHVEAPTGGSVILAAIMLKMGGYGFLRFSLPITPDAARELDW
                     LVITLSLTAVVYIGFVALVQQDMKKLIAYSSIAHMGFVTLGAFVAFDILRATGGVQGA
                     AMGLDGAMVQMVSHGLVSGALFFCIGVLYDRVHSRQIADYGGVINTMPVFGAFAVLFA
                     MANAGLPGTSGFVGEFLVILASFKANFWYAFLAAVTLILGAAYTLWLVKRVVFGPVGN
                     DHVAHLKDLNGREFAVLGVLALAVLALGLWPAPLLDVMRPTLEQLVQQMMVTKL"
     gene            complement(3099..>4122)
                     /locus_tag="VD737_09290"
     CDS             complement(3099..>4122)
                     /locus_tag="VD737_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXW10798.1"
                     /translation="LFLAAGSVIIAMHHEQDMRNMGGLRRYMPVTYWTALVGTLALAG
                     FPGFSGFFSKDALIEAVHGSTIPGAGYAYFCVVIGVFVTAFYSFRLLFLTFHGQERFA
                     TAGEAHHEAIESTHVATDADAGHEPDVSDSGGHAHDDHAHGDHDHGGPPRESPWVVTV
                     PLVLLAIPSVIIGWLTIEPLLFGGYFGDSIRVAAQHDVLRRLGEEFHGPAQFVLHGLM
                     GPAVWLAAAGAALAWFLYLKRPELAETLRQKAGALYTLLVNKYYFDWFNEKVLAAGSR
                     GLGQALWKGGDVALIDGAAVNGSARLVGWVAQVARGLQSGYLYHYAFATIIGLSILLA
                     WLLLRS"
BASE COUNT          843 a         1395 c         1346 g          538 t
ORIGIN      
        1 cgaccttcgg gttatgagcc cgacgagctg ccagactgct ccaccccgca atcgagccgc
       61 ctattatgca caggcagcaa ctttaggcaa acgatttcgg ccgcgagccg ttacggaatg
      121 acccgggcgc acagcgccag cagcatgccc gggaacagcc ccagcacgag cacggcgaga
      181 ccgttcacgc tcagcacgaa tcgcatcgcg ccgctgccct cgatcgccgc gcgctcgacc
      241 ggctcgtcga agtacatgag cttcacgacg cgcaggtagt agtacgcgcc gatcaccgag
      301 aacgcgaccg cgaggatcgc gagccacacg ctgccgacgt gcagcaccga ctggatcacg
      361 gccagcttgg cccagaatcc tacgaatggc ggcacgccgg ccatgctgaa catcagcatc
      421 agcatcacag cagcgaacca cgggctgcgc gcattcagac cacggtagtc gtcgaggctg
      481 tcggcctcga agcccgcgcg cgacaacagg atcaccacgc cgaagccgcc agccgacatc
      541 agcacgtaca cgatcgtgta atacagcgct gactcgtagc cctcgctggt cccggccagg
      601 atgccgaaca ggatgaagcc cacgtgcgaa atcgtcgagt acgcgagcat gcgcttgatg
      661 ttgttctgcg cgatcgcgac gacgttgccc aggacgatcg aggcgacggc gacgaacgtg
      721 atcatgtccc gccagcccga gtcgtggatc ggcccgagcc cctcggccag cagccggaag
      781 gcgagcgcga acgagccgag cttcggtgcg gcggccagga acagcgtcac cggtgtcggc
      841 gcgccgtgat agacgtccgg gatccacatg tggaacggca ccgcgccgaa cttgaacgcg
      901 acgccgacga cgatgaaggc caggccgaac agcaacccga tctgcccggc cggcatctga
      961 gaggctactg cgctgacgac gccgagttcg agtgaccccg tcacgccgta gatgatcgac
     1021 atgccataca gcaacgcgcc ggatgcgatc gagccgagca cgaaatactt gatggccgat
     1081 tccgcggcga cgccggactc gcggttgaag gccaccatcg cgtactgcga cagcgccagc
     1141 agttcgacgc ccaggtacag cgtcacgagg ctgttggccg agaccagcac catgatcccg
     1201 agcagcgcaa acaagccgag cacgaagtac tcgcctttca acagccgtcg gacacgcaga
     1261 tagtcatagg cgtacaagaa cgcgactgcg acggccgcga ctgcgacgac cttgagcagg
     1321 gtcgacaacg gatccgccac gtaggtgccg tgccagccga ccgtccgctc ggtgaatgcg
     1381 aacatggtcg tgacgaggga ggtgccggcg agtgccacga gcgacagcgc gaacgtgacc
     1441 cagcgcgagc gatcgctcag gaacaggtcg accagcagca cgacgcacgc cgcaccgagc
     1501 aggaacatct ccggcgccgc gagcagcagg tcgagtcggg taatggatgc agggttcatg
     1561 gtctagagct tcgtcaccat catctgctgc acgagctgct cgagcgtcgg acgcatcacg
     1621 tcgagcagcg gcgccggcca caggcccagc gccagcaccg caagcgccag cacgccgagc
     1681 accgcgaact cacgcccgtt caggtccttc aggtgcgcga cgtggtcgtt gcccacgggg
     1741 ccgaacacga cgcgcttgac cagccagagc gtgtaggcgg cgcccaggat cagcgtcacc
     1801 gcggcgagga aggcgtacca gaagttcgcc ttgaacgagg ccaggatgac cagaaactct
     1861 ccgacgaacc cggacgttcc gggcaggccg gcgttggcca tcgcaaacag cacggcgaac
     1921 gcgccgaaca ccggcatcgt gttgatcacg ccgccgtagt cggcgatctg ccggctgtgc
     1981 acgcggtcgt agagcacgcc gatgcagaag aacagcgcgc ccgagacgag gccgtgcgag
     2041 accatctgca ccatcgcgcc gtcgagcccc atggcggcgc cctgcacgcc cccggtcgcg
     2101 cgcaggatgt cgaacgcgac gaaggcgccg agcgtcacga accccatgtg cgcgatcgac
     2161 gagtacgcga tcagcttctt catgtcctgc tgcacgagcg cgacgaagcc gatgtagacg
     2221 acggcggtca gtgacagcgt gatgaccagc cagtcgagtt cgcgtgccgc atccggcgtg
     2281 atcggcaggc tgaagcgcag gaagccgtag ccgcccatct tcagcatgat ggccgccagg
     2341 atcacggagc cgccggtggg cgcctcgacg tgcgcgtccg gcagccaggt gtgcaccggc
     2401 cacatcggca ccttgaccgc gaacgcgagg aagaacgcga cgaagatcag cacctgctcg
     2461 cgcaggccga gcggcagtgc ctgcaggtcg gcgatcgagt agctgccgcc cttcaggtac
     2521 aggtagatca gcgcgaccag catcagcacg gagcccagga acgtgtagag gaagaacttg
     2581 accgtcgcgt acacgcgccg cggtccgccc cagacgccga tgatcacgaa catcgggatc
     2641 agcatggctt cccagagcac gtagaacagc agcgcgtccg tcgcggcgaa gacgccgatc
     2701 atcaaccctt cgaggatcag gaacgaggcc aggtactgtg ccggccgctt ctcgatcacc
     2761 gtccagccgg cgatcacgac cagcggcgtg atgaaggccg tcagcacgat cagcggcagc
     2821 gagatgccgt cgacgccgag cgaataccag gcgttgaagg cgccgatcca cggctgcagc
     2881 tcgacgaact gcatcgacgc gaccgacgga tcgaagtcgt tccagaggat cacgcccagg
     2941 gcgaacgtga ccagcgacgc ggcgagcgcg atccagcgcg cgagcggcgc gttgcggtcg
     3001 ccgacgagga gcagcagcac gcccgccacg atgggcagcc agacgagcac gctcaggatg
     3061 ggccagtcag cttgcattgt tttcgtcgtt gtccgaggtc agcttcgcag gagcagccac
     3121 gcgagcagga tcgagagccc gatgatcgtc gcgaacgcgt agtggtacag gtagccggac
     3181 tgcaggcccc gggcgacctg tgccacccac cccacgagcc gtgccgagcc gttgacggcc
     3241 gcgccgtcga tcaatgcgac gtccccgccc ttccagagcg cctggccgag gccacggctg
     3301 cccgccgcca gcaccttctc gttgaaccag tcgaagtagt acttgttgac gagcagcgtg
     3361 tagagcgcac ccgccttctg gcgcagcgtt tcagcgagct ccggccgctt caggtacagg
     3421 aaccaggcca gcgccgcgcc agccgcggcc agccagacgg ccgggcccat caggccgtgc
     3481 agcacgaact gtgccgggcc gtggaactcc tcgccgagtc ggcgcagcac gtcgtgctgc
     3541 gcggcgacgc ggatcgaatc gccgaagtag ccgccgaaca gcagcggctc gatcgtcagc
     3601 cagccgatga tcacggaggg aatcgcgagc agcacgagcg gcaccgtgac gacccaaggg
     3661 ctttcccggg gtggaccgcc gtgatcgtgg tcgccgtgcg catgatcgtc gtgggcatgg
     3721 ccgcccgagt cggagacgtc gggctcgtgg cccgcatcgg cgtcggtggc gacgtgcgtg
     3781 ctctcgatgg cctcgtgatg ggcctcgccc gccgtcgcaa agcgctcctg tccgtggaac
     3841 gtcaggaaca gcagccggaa cgaatagaac gccgtgacga acacgccgat cacgacgcag
     3901 aagtacgcgt agcccgcgcc tgggatcgtc gagccgtgca cggcctcgat cagggcgtcc
     3961 ttggagaaga agccggagaa cccgggaaac cccgccagcg cgagcgtgcc gaccagcgcg
     4021 gtccagtacg tcacgggcat gtaccggcgc aacccgccca tgttgcgcat gtcctgctcg
     4081 tggtgcatcg cgataatcac cgagcccgcc gcgaggaaca gc
//
LOCUS       DASPFR010000351         4117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1326548, whole genome shotgun sequence.
ACCESSION   DASPFR010000351 DASPFR010000000
VERSION     DASPFR010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326548"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1273)
                     /locus_tag="VD737_09295"
     CDS             complement(149..1273)
                     /locus_tag="VD737_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXW10799.1"
                     /translation="MPSDEQDAAALVGAAFDGPIDVIGDVHGEIGALEDLLHVLGYAG
                     PGEHPQARRLVFIGDLCDRGPDSPAVIRRVQAMVAAGTAQCLLGNHELNVLRGVRKEA
                     NGWFFADDHDRRRGLYLDARRLGDDDEARREVRRFFASLPVVLERADLRLVHACWDDD
                     RIGELRGLHGRANAVELYRRYAQRSDDRARETGRYEQVQALLERYGPHLHDPSASVPM
                     LDAVAEHDVDYQMSNPLRVLTSGTEHRAAAPYYLAGKWRMVERGEWWRSYAAATPVLF
                     GHYWRWPTPDTGERMGRHARDPFPDRAAHEWLGARDTAFCNDYCVGARFAERQGWPGE
                     PFRTRLGAVRWPERELLFDDGRGVELATAEADRGSLRTRG"
     gene            complement(1425..>4117)
                     /locus_tag="VD737_09300"
     CDS             complement(1425..>4117)
                     /locus_tag="VD737_09300"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXW10800.1"
                     /translation="RTVCLEFFGQARDAIPAIVEIRDELARQSRDRGVQLAGLEHLDE
                     RYLKAVGYTTKSRRGVLPKMVLIGDVAGADENAVARCAAEVVRIANARSGEGFVAVGP
                     EARRAFWRDRSRTAAIARHTNAFKLNEDVVIPLERLGEYTDAIERINVELSLANKLRL
                     LDELEAYLRGEPRIGKTGDADVDRLPRDEVLGGRVEQALTALDACRLRWRWLAEQLDR
                     PLVEVPDELRDIGLADVATAVEAGGLGNAPQRRVFDLLQDRTIRVSWKRELRGELRRI
                     FAGGPFVPLVADLEAVHRAVLHGRVFVALHMHAGDGNVHTNIPVNSDDYEMLQTAYRV
                     VARIMQVARRLDGVISGEHGIGITKLEFLADAEIAGFRDYKQRVDPEGRFNAGKLLPG
                     ADLRNAYTPSFNLLGHESLIMQQADIARISDAIKDCLRCGKCKPVCSTHVPRANLLYS
                     PRNKILATSMLVEAFLYEEQTRRGVSLQHWDAFGDVAEHCTVCHKCEQPCPVDIDFGD
                     VSMAMRDVLRRMGHARANPAKSAAMFFLNTNDPRTIRVTRKLMIDCGYKAQRLGAGTL
                     GKLAGAQTKRPPATTGRSPLREQVVHFVNKKLPGGLPSRTARALLDLEDRNYVPVIRD
                     PARTSTDSEAVFYFPGCGSERLFSQVGLATQAMLWHAGVQTVLPPGYLCCGYPQRGAG
                     QFDQAEKIITENRVLLHRVANTLNYLDIRTVVVSCGTCFDQLQGYEFDQIFPGTRILD
                     IHEFLLEKGIRLEQTTGVRYTYHDPCHSPMKTREPLEVVNALLRNEGGGAIARNERCC
                     GESGTFAVTRPDVATQVRFRKQQEVERAAARLREGHDGPVKVLTSCPSCLQGLSRYDD
                     DAGTSADYVVVEMARHILGPQWLEAYVRQANSGGIERVLL"
BASE COUNT          588 a         1438 c         1444 g          647 t
ORIGIN      
        1 cgttttcaag tactgctcgg gctacgcggc tacctgacgt tgatcaactc cgacacggac
       61 ctgttctgcg cgagcaacgc cgagcaggcg agttgcctca tccggtcctc gggcagcacc
      121 tttttccaga ccgagggcca actcggcttc agcctcgtgt tctgagcgag ccgcggtccg
      181 cctcggccgt tgcgagctcg acgccccggc cgtcgtcgaa cagcagttcg cgctcgggcc
      241 agcgcaccgc cccgagccgc gtgcgaaacg gttcgccggg ccagccctgg cgctcggcaa
      301 accgcgcacc gacgcagtag tcgttgcaga acgccgtgtc gcgggcgccg agccactcgt
      361 gggcggcgcg gtccgggaac ggatcgcggg catgccgtcc catgcgctcg ccggtgtccg
      421 gcgtgggcca gcgccagtaa tggccgaaca ggacgggcgt ggcggccgcg tacgagcgcc
      481 accactcgcc gcgttcgacc atgcgccact tgcccgccag gtaatacggc gcggccgcgc
      541 gatgctcggt tccggacgtc agcacgcgca acggattcga catctggtag tcgacgtcgt
      601 gctcggcaac ggcgtccagc atcggcaccg acgccgacgg atcgtgcaga tggggaccgt
      661 agcgctccag caaggcctgg acctgctcgt accggcccgt ctcccgtgcg cgatcgtccg
      721 agcgctgcgc gtagcgtcga tacagctcga ccgcattggc gcgaccgtgc aagccgcgca
      781 gctccccgat gcggtcgtcg tcccagcagg cgtgcacgag ccgcaggtcg gcgcgctcga
      841 gcacgacggg caggctcgcg aagaagcgcc gcacctcacg gcgcgcctcg tcgtcgtcgc
      901 cgagccgccg cgcatcgaga tacagccctc gtcgccggtc gtggtcgtcc gcgaagaacc
      961 agccattcgc ctccttgcgc acgccgcgca gcacgttcag ctcgtggttg ccgagcaggc
     1021 actgcgcggt gccggccgcc accatcgcct gcacgcggcg gatcaccgcg gggctgtcgg
     1081 ggccacgatc gcagagatcg ccgatgaaca cgagccggcg cgcctggggg tgttcgcctg
     1141 ggcctgcata acccaggaca tgcagcaggt cctcgagcgc cccgatctcg ccgtgcacgt
     1201 cgccgatcac gtcgatcggg ccgtcgaacg ccgcgcccac gagcgcagct gcgtcctgtt
     1261 cgtccgaggg cacgggccgg ctcatagcgc agggaccgcg cgctcgtcgc gcagttccgg
     1321 cggcgtcccg gtgaacggcg cgcgcagcgc gatcccgcgg tcttgcggcg tcgagagcgc
     1381 gtcgcgctgc cgcacgtgcg cccggcggcg gagcgggtgc ggcttcagag gagcacgcgc
     1441 tcgatgccgc ccgagttggc ctgtcgcaca taggcctcga gccattgcgg cccgaggatg
     1501 tgccgcgcca tttcgacgac gacgtagtcc gcgctcgtgc cggcgtcgtc atcgtaccgc
     1561 gatagtccct gcaggcacga cgggcacgac gtcagcacct tgacgggccc gtcgtgtccc
     1621 tcgcggagcc gtgcggcagc gcgctcgacc tcctgctgct tgcgaaagcg gacctgcgtg
     1681 gcgacgtccg gacgcgtgac cgcgaacgtg cccgactcgc cgcagcagcg ttcgttcctg
     1741 gcgatcgcgc cgccgccctc gttgcgcagc agtgcgttga ccacctcgag cggctcgcgc
     1801 gtcttcatcg gggagtggca cggatcgtgg taggtgtatc ggacgccggt cgtttgttcg
     1861 agccggatgc ccttctcgag caggaactcg tggatgtcga ggatgcgtgt gcccgggaag
     1921 atctggtcga attcgtaacc ctgcagctgg tcgaagcacg tgccgcagct cacgacgacg
     1981 gtacgtatgt cgaggtagtt gagcgtgttg gccacgcggt gcagcagcac gcggttctcg
     2041 gtgatgatct tctcggcctg gtcgaactgc ccggcgccgc gttgcgggta gccgcagcag
     2101 aggtagcccg gcggcagcac ggtctgcacg ccggcgtgcc agagcatggc ctgcgtcgcg
     2161 aggccgacct gggagaacag ccgttccgag ccgcagcccg gaaaatagaa caccgcttcg
     2221 gagtcggtgc tggtgcgcgc cggatcgcgg atcaccggca cgtagttgcg gtcctcgagg
     2281 tcgagcagcg cacgtgccgt cctgctcggc aggccgccgg gcagcttctt gttgacgaag
     2341 tgcacgacct gctcgcgcag cggtgagcga ccggtcgtcg ccggcggccg cttcgtctgt
     2401 gcaccggcca gcttgccgag cgtcccggcg ccgagccgtt gcgccttgta gccacagtcg
     2461 atcatcagct tgcgcgtgac gcggatggtg cgcggatcgt tcgtgttcag gaagaacatg
     2521 gcggccgact tcgccggatt ggcgcgcgcg tgccccatcc ggcgcagcac gtcgcgcatc
     2581 gccatcgaga cgtcgccgaa gtcgatgtcg accgggcagg gctgctcgca cttgtggcag
     2641 acggtgcagt gctccgcgac gtcgccgaac gcgtcccagt gctgcagcga gacgccgcga
     2701 cgcgtctgct cctcgtagag gaacgcctcg accagcatcg aagtcgcgag gatcttgttg
     2761 cgcggcgagt agagcaggtt ggcgcgcggc acgtgcgtcg agcagacggg cttgcacttg
     2821 ccgcagcgca ggcagtcctt gatcgcgtcg ctgatcctgg cgatgtcggc ctgctgcatg
     2881 atcagcgact cgtggccgag caggttgaag ctcggcgtgt aggcgttgcg caggtcggcg
     2941 ccgggcagca gcttgccggc gttgaagcgg ccctcggggt cgacgcgctg cttgtagtcg
     3001 cggaagcccg cgatctcggc gtcggcgagg aactcgagct tggtgatccc gatgccgtgc
     3061 tcgcccgaga tcacgccgtc gagacgccgc gcgacctgca tgatgcgcgc gacgacccga
     3121 taggccgtct gcagcatttc gtaatcgtcg gagttgaccg gaatgttggt gtggacgttg
     3181 ccgtcgcccg cgtgcatgtg cagcgcgacg aacacgcggc cgtgcagcac cgcgcgatgg
     3241 acggcctcga ggtccgccac cagtggcacg aacggcccgc ccgcgaagat ccggcgcagc
     3301 tcgccgcgca gctcgcgttt ccaggaaacg cggatcgtgc ggtcctgcag caggtcgaac
     3361 acccgccgtt gcggcgcgtt ccccagcccg cccgcctcga cggcggtggc cacgtccgcc
     3421 aggccgatgt cccgcagttc gtccggcacc tcgacgagcg gccggtccag ctgctcggcg
     3481 agccagcgcc agcgcagccg gcacgcgtcc agggccgtga gcgcctgctc gacgcgaccg
     3541 ccgaggactt cgtcccgcgg cagccggtcg acgtcggcgt cgccggtctt gccgatgcgt
     3601 ggctcgccgc gcaggtaggc ctcgagctcg tcgagcagcc gaagcttgtt cgcgagcgac
     3661 agctcgacgt tgatgcgctc gatcgcgtcc gtgtattcgc cgaggcgctc gagcgggatg
     3721 accacgtcct cgttgagctt gaacgcattg gtgtgacgtg cgatcgccgc ggtgcggctc
     3781 cggtcgcgcc agaacgccct gcgcgcctcg ggacccacgg ccacgaagcc ctcgcccgac
     3841 cgcgcgttgg cgatgcgcac gacttcggcc gcgcagcgcg cgaccgcgtt ctcgtcggca
     3901 ccggcgacgt cgccgatcag gaccatcttc ggcagcacgc cgcgccgcga cttggtggtg
     3961 tagcccaccg ccttgaggta gcgctcgtcc aggtgctcga gccccgcgag ctggacgcca
     4021 cggtcacgtg actggcgcgc gagttcgtcg cggatctcga cgatcgcggg gatcgcgtcg
     4081 cgggcctggc cgaagaactc caggcacacg gtgcgca
//
LOCUS       DASPFR010000352         2828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1569215, whole genome shotgun sequence.
ACCESSION   DASPFR010000352 DASPFR010000000
VERSION     DASPFR010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569215"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VD737_09305"
     CDS             <1..887
                     /locus_tag="VD737_09305"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HXW10801.1"
                     /translation="PRGKTVVEIRPDRIILVDIARAGDRLVAVGERGFALISDDAGKT
                     WRARETPVTRTLTGVAFRDPKVGVAVGHGASVVRTEDGGETWTQVPLAEAEPESLLGV
                     VSLGGDRFAAYGAFGMYFDSADAGRTWERRTVMSEEFEWHISQVLPVGDSLLLVGESG
                     TLARSDDGGVTWTEITSPYVGSYFGAVATPDGAVLAFGMRGNVFRTSDLGATWTEVPL
                     DPTASLNGGTTLSDGRILLVGNSGLLAVSRDGGQTLETHWAPGGRGFSNVVEANGAII
                     LAGESGITVLDPDWLARR"
     gene            904..>2828
                     /locus_tag="VD737_09310"
     CDS             904..>2828
                     /locus_tag="VD737_09310"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HXW10802.1"
                     /translation="MNDSNLRAGDNGNGDGGEIRRQQFSADWVGKISYWIFHHRKPLL
                     ILFVLITILLGAMASQLRVQAAFTKMIPLKHPYMQTFLQYQSDFGGANKVLVALKNNK
                     GDIYEKEFMETLRKVTEEVFYIKGVERSSVASLFTPNVRYNEVVEDGFRGGNIVSADF
                     AGTPEQLQLVRENLLKSDWIGRLVSNDHSSAMVVATLMENDPETGERLDLQEVAAELE
                     KIRAKYEQGDTTVHVIGFAKAVGDIAKGAAGVLLFFGLAFVITSILLFWYSGSLMITA
                     LALICAIVPVIWLLGLLPVFGLGLDPMSILVPFLIFSIAVSHAVQMTNAWKLETLHGA
                     DGITASTHSFQKLFIPGAMALLANALGFMVIAFVEIEIVRELAYTATIGVTVMIITNK
                     MLLPILLSYYRFTPEQASKLAGKETAGDWLWERIGPLATKRIAWVPILAGVALLVFGL
                     SQVRHLHIGDLGKGVPELRPESRYNQDVEMITSHFAIGVDLLQVIAEAKPENDEDSPC
                     VDRAVMDKIEDFEFQMRQVDGVATVRSLSGFVKSITQSFAETYSKWRMLPESRAQIAQ
                     GVGYATRLGNEFMNSRCTAMPVNIYTSDHQATTIEHVVDRIKAFKEAGGDDERITFRL
                     ASGNVGVMAATNEAVHAA"
BASE COUNT          465 a          931 c          966 g          466 t
ORIGIN      
        1 cgccgcgcgg caagacggtc gtcgagatcc gtcccgaccg catcatcctg gtcgacatcg
       61 ctcgcgcggg agatcgcctg gtcgccgtgg gcgagcgcgg ctttgcgctg atctcggacg
      121 atgccggcaa gacctggcgc gcgcgcgaga cgcccgtcac tcgcacgttg accggcgtcg
      181 ccttccgcga tcccaaggtc ggcgtcgcgg tcggtcacgg ggcctcggtc gtccggaccg
      241 aggacggcgg cgagacctgg acccaggtgc cgctggccga ggccgagccc gagtcgctgc
      301 tcggtgtcgt gagcctcggc ggcgatcgct tcgccgcgta cggcgccttc ggcatgtact
      361 tcgattcggc ggacgcgggg cgcacctggg agcgccgaac cgtgatgtcg gaggaattcg
      421 agtggcacat ctcgcaggta ctgccggtcg gcgattcgct gctgctggtc ggggagtccg
      481 gcacgctcgc gcggtccgat gacgggggtg tcacctggac cgagatcacc tcgccgtacg
      541 tcggctcgta cttcggcgcg gtcgccacgc ccgacggcgc ggtgctcgcg ttcggcatgc
      601 gcggcaacgt cttccgcacg agcgacttag gcgccacctg gacagaagtg ccgctcgatc
      661 ctacggcgtc gctcaacggc ggcacgaccc tcagcgacgg gcgcatcctg ctggtcggca
      721 actccgggct gctcgcggtg agccgcgacg gcggacagac gctcgagacg cactgggcgc
      781 ccggcgggcg cggtttttcg aacgtcgtcg aggccaacgg ggcgatcatc ctggcgggcg
      841 agagcggcat cacggtgctc gacccggact ggctcgcgcg ccgctgatag gagaggagcg
      901 cacatgaacg acagcaacct ccgggccggc gacaacggca atggagacgg cggcgagatc
      961 cggcgccagc agttctccgc ggactgggtc ggcaagatct cctactggat cttccaccac
     1021 cgcaagccgc tgctgatcct gttcgtgctg atcacgatcc tgctcggcgc catggcgagc
     1081 cagcttaggg tgcaggcggc gttcaccaag atgatcccgc tgaaacaccc gtacatgcag
     1141 acgttcctgc agtaccagtc cgacttcggc ggcgccaaca aggtgctggt cgcgctcaag
     1201 aacaacaagg gcgacatcta cgagaaggag ttcatggaga cgctccgcaa ggtgacggag
     1261 gaggtcttct acatcaaggg cgtcgagcgc agctcggtcg cctcgctgtt cacgcccaac
     1321 gtccgctaca acgaggtcgt cgaggacgga ttccggggcg gcaacatcgt ctccgcggac
     1381 ttcgccggca cgccggagca gctgcagctc gtgcgcgaga atctgctcaa gtcggactgg
     1441 atcgggcggc tcgtctcgaa cgaccactcg tcggcgatgg tcgtcgcgac gctgatggag
     1501 aacgaccccg agaccggcga gcggctcgac ctgcaggagg tcgccgcgga actcgagaag
     1561 atccgcgcca agtacgagca gggcgacacc acggtccacg tgatcggctt cgccaaggcg
     1621 gtcggcgaca tcgccaaggg cgcggccggc gtgctgctgt tcttcgggct ggctttcgtc
     1681 atcacctcga tcctgctctt ctggtactcg ggctcgctga tgatcacggc gctcgcgctg
     1741 atctgcgcga tcgtgccggt gatctggctg ctcgggttgc tgccggtgtt cggcctgggc
     1801 ctcgacccga tgtcgatcct cgtgccgttc ctgatcttct cgatcgccgt gtctcacgcg
     1861 gtgcagatga ccaacgcctg gaagctcgag acgctgcacg gcgccgacgg catcacggcg
     1921 tcgacccact cgttccagaa gctgttcatc ccaggcgcga tggcgctgct cgccaacgcg
     1981 ctcggcttca tggtgatcgc gttcgtcgag atcgagatcg tgcgcgagct cgcctacacg
     2041 gcgacgatcg gcgtgacggt gatgatcatc accaacaaga tgctgctgcc gatcctcctc
     2101 tcgtactaca ggttcacgcc cgagcaggcg tcgaaactcg ccggcaagga gacggcgggc
     2161 gactggctct gggagcgcat cggtccgctc gcgacgaagc ggatcgcctg ggtgccgatc
     2221 ctggccggtg tggcgctgct ggtgttcggc ctgtcgcagg tgcggcacct gcacatcggc
     2281 gacctcggca agggcgtgcc ggagctgcgg cccgagtcgc gctacaacca ggacgtcgag
     2341 atgatcacga gccacttcgc gatcggcgtg gacctgctgc aggtgattgc cgaggccaag
     2401 cccgagaacg acgaggattc gccctgcgtc gaccgtgccg tgatggacaa gatcgaggac
     2461 ttcgaattcc agatgcggca ggtcgacggc gtggcgaccg tccgcagcct gtcaggcttc
     2521 gtcaagtcga tcacccagtc gttcgccgag acctattcca agtggcgcat gctgcccgag
     2581 agccgcgcgc agatcgcgca gggcgtcggc tatgcgacgc gtctcggcaa cgagttcatg
     2641 aattcgcgct gcacggcgat gccggtcaac atctatacgt ccgaccacca ggccacgacg
     2701 atcgagcacg tcgtcgatcg catcaaggcg ttcaaggagg ccggcggcga cgacgagcgc
     2761 atcacgttcc ggctcgcgag cggcaacgtc ggcgtgatgg ccgcgaccaa cgaggcggtg
     2821 cacgcggc
//
LOCUS       DASPFR010000353         3305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1081573, whole genome shotgun sequence.
ACCESSION   DASPFR010000353 DASPFR010000000
VERSION     DASPFR010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081573"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VD737_09315"
     CDS             <1..685
                     /locus_tag="VD737_09315"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HXW10803.1"
                     /translation="SLGWWQIGRGREKQALIESFARGTQSSVELAGDVTVDELPRFQH
                     VRAIGHYEPSRQLLVDNMPSQAGRPGYRVLTPFVRDGAARLLLVDRGWVPLGASRDEL
                     PRVADVTTERRAVSGRLDQLPAPGVRVGQASTPGDQRWPRVLNFPLPRDLEQALGQPV
                     ESRIVLLDPAAPDGYERVWRPSLGSGPERHLGYAIQWFAFAIVALVLFVALSLRREPG
                     VTPEDEPAA"
     gene            682..1353
                     /locus_tag="VD737_09320"
     CDS             682..1353
                     /locus_tag="VD737_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10804.1"
                     /translation="MSSTNETGATSRAPGEGSSGVDARRRRGRRQLLVLAAIFFVPLA
                     VAFWLYYGPGEWRPVGGTNQGDLIEPAVPLAEVELARTDGTQSSSRELFRGKWTMAYL
                     GPGRCDERCRRALYLSRQSRIALNKDMDRVQRVFLATDDCCDRDFLQAEHPDLVVVPL
                     GDDAASRTLLRQFPVLGDTEATEAGRLWVIDPLGNLVLSYSERAPDKALLTDLRKLLR
                     LSHIG"
     gene            1369..2379
                     /locus_tag="VD737_09325"
     CDS             1369..2379
                     /locus_tag="VD737_09325"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HXW10805.1"
                     /translation="MRRLVLFRRLCLLGTILALCVVVLGAYVRLSHAGLGCPDWPGCY
                     GHLTAGEAAANEHTVNAAFPHRPIEYAKALKEMLHRYLASALGLLILALAAVAWTNRR
                     NAQQPVRLPLALVALVIFQGLLGMWTVTLLLKPAIVTAHLFGGLATMSLLWWMALATD
                     RRSRPAAERTLRTWAVVGAVVLAVQIFLGGWVSTNYAAVACPDFPTCQRSFWPTMDFR
                     DAFVLWRGLGIDYEGGVLDHPARVAIHHTHRLGAIVTAFVLGIVAWKAVRAGQSLGVR
                     TAGAALGIALVAQWLVGPIMVLQTFPLPLATAHNAIAAVLVLCMVALLRFLRPPRHAA
                     VY"
     gene            2456..>3305
                     /gene="cyoE"
                     /locus_tag="VD737_09330"
     CDS             2456..>3305
                     /gene="cyoE"
                     /locus_tag="VD737_09330"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HXW10806.1"
                     /translation="MNLAGPNLVPGAAATWRDFYELGKPKVVALIVFTAVVGMFLAVP
                     GVPPWPALLYGTLGIGLAASSAAAINHVVDRRFDEKMARTMNRPIPTGHVTTRQALVY
                     AAIIGTVSMLLLWGLVNPLTAVLTFLSLIGYAVLYTVWLKHVTPQNIVIGGAAGAAPP
                     VLGWTAVTGSADPHSLLLFLIIFAWTPPHFWALAIARRADYEKAGIPMLPVAYGVEFT
                     RLHILLYTILLFIVTLLPYLTGMSGLLYLAAAIVLNVIFTRHAVRLRRPDAPVQLPMQ
                     VFRYSIN"
BASE COUNT          425 a         1185 c         1136 g          559 t
ORIGIN      
        1 ctcgctgggc tggtggcaga tcgggcgcgg ccgcgagaag caggcgctga tcgagtcgtt
       61 cgcgcgcggc acgcaatcga gcgtcgagct cgcgggcgac gtgaccgtcg acgagctgcc
      121 gcgcttccag cacgtgcgcg ccatcggtca ctacgagccg tcgcgccagc tcctggtcga
      181 caacatgccg tcgcaggcag gcaggccggg ctaccgcgta ctgacgccgt tcgtgcgcga
      241 cggtgccgcg cgcctgttgc tcgtcgaccg tggctgggtg ccgctcggcg cgtcccgtga
      301 cgagctgcca cgggtcgccg acgtgacgac ggagcgtcgc gcggtgtcgg gtcggctcga
      361 ccagctgccg gcgccgggcg tgcgcgtggg ccaggcttcc accccggggg atcagcgctg
      421 gccgcgcgta ctgaacttcc cgctgccacg cgatctggag caggccctcg ggcaaccggt
      481 cgagtcgcgc atcgtgctac tggatccggc agcaccggac ggatacgagc gcgtgtggcg
      541 cccgtcgctg ggttccggcc ccgaacggca cctgggctat gcgatccagt ggttcgcgtt
      601 cgcgatcgtc gcgctggtgc tcttcgtcgc gttgagcctg cgacgcgagc ctggcgtcac
      661 ccccgaggac gaacccgccg catgagctcg accaacgaaa ccggcgccac gtcgcgcgct
      721 ccgggcgagg gcagcagcgg cgtagacgcg cggcggcggc gcggccgccg ccagttgctg
      781 gtgctcgccg cgatcttctt cgttccgctc gcggtggcgt tctggctgta ctacggcccg
      841 ggtgagtggc gtccggtcgg cggaacgaac cagggcgacc tgatcgagcc ggcggtgccg
      901 ctcgccgagg tcgagctcgc gcgcacggac ggcacgcaga gcagctcgcg ggagctgttt
      961 cgcggcaagt ggacgatggc gtacctcggg cccggacgat gtgacgagcg gtgccgccgc
     1021 gcgctgtacc tgtcgcggca gtcgcggatc gccctcaaca aggacatgga ccgcgtgcag
     1081 cgcgtgttcc tcgcgaccga cgactgttgc gaccgcgatt tcctgcaggc cgagcatccc
     1141 gacctcgtcg tcgtgccgct cggggacgac gcggcctcgc ggacgctgct ccgacagttc
     1201 ccggtcctcg gcgacacgga ggcgacggaa gcgggccgac tgtgggtcat cgacccgctc
     1261 ggcaacctcg tgctgagcta ctccgagcgt gccccggaca aggcgctgct cacggacctc
     1321 aggaaactgc tgcgcctgtc ccacatcggt tgacgcgagg attcctggat gcgtcgtctc
     1381 gtgctctttc gtcgcctgtg cctgctcggc accatcctcg cgctgtgcgt cgtggtactc
     1441 ggtgcctacg tgcgcctgag tcatgccggg ctcggttgcc ccgactggcc gggctgctac
     1501 ggtcacctga ccgccggcga ggcggccgcg aacgagcaca ccgtcaacgc cgcttttccg
     1561 caccggccga tcgagtacgc gaaagcgctc aaggaaatgc tgcaccgcta tctcgcgagc
     1621 gcgctggggc tgctgatctt ggcgctcgcg gcggtggcgt ggaccaaccg ccgcaatgca
     1681 cagcaaccgg tgcggctgcc gctggccctg gtcgcgctcg tgatcttcca gggcctgctc
     1741 gggatgtgga cggtcacgct gctgctcaag cctgccatcg tgacggcaca cctgttcggc
     1801 gggctcgcca cgatgtcgct gctctggtgg atggcgctcg cgaccgatcg gcgctcgcgg
     1861 cccgccgccg agcgcaccct gcggacgtgg gccgtcgtcg gcgcggtcgt gctcgccgtg
     1921 cagatcttcc tcggtggctg ggtcagcacc aattacgccg ccgtggcgtg ccccgacttc
     1981 ccgacctgcc agcgctcgtt ctggcccacg atggacttcc gcgacgcgtt cgtgctgtgg
     2041 cgcggtctcg gcatcgatta cgagggcggc gtgctcgacc atcccgcgcg cgtcgcgatt
     2101 caccacacgc accggctcgg cgcaatcgtc acggccttcg tgctcgggat cgtggcctgg
     2161 aaggccgtgc gcgcaggcca gtcgctcggc gtgcgcaccg caggagccgc actcggcatc
     2221 gcgctcgtag cgcagtggct cgtcgggccc atcatggtac tgcagacgtt cccgctgccg
     2281 ctcgccacgg cgcacaacgc gatcgccgcg gtgctggtgt tgtgcatggt ggcgctgttg
     2341 cgcttcctgc gaccaccgcg tcacgccgcc gtctactgag gacgcgtcga tgccagtgac
     2401 gcacgccacc acgggcgcct gcgatccggg ccggggcggt agaatcgctc ccaccatgaa
     2461 cctggccggc cccaacctcg tccccggagc cgccgcgacc tggcgcgatt tttacgaact
     2521 cggcaagccc aaagtcgtcg cgctgatcgt cttcacggcc gtagtcggca tgttcctcgc
     2581 agtgccggga gtgccgccct ggccggcgct gctgtacggc acgctgggca tcgggctcgc
     2641 cgcgtcgagc gcggcggcga tcaaccacgt cgtcgaccgc cgcttcgacg agaagatggc
     2701 gcgcacgatg aaccgcccca tcccgaccgg gcacgtcacg acgcgccagg cgctcgtcta
     2761 cgccgcgatc atcggcaccg tctcgatgct gttgctctgg gggctggtca acccgctcac
     2821 cgcggtgctc acgttcctgt cgctgatcgg gtacgccgtg ctctacacgg tctggctcaa
     2881 gcacgtgacg ccgcagaaca tcgtcatcgg cggcgccgcg ggcgcggcgc ccccggtgct
     2941 cggctggacg gccgtgacgg gctcggccga cccgcactcg ctgctgctgt tcctgatcat
     3001 ctttgcctgg accccgccgc atttctgggc gctcgcgatc gcgcggcggg ccgactacga
     3061 gaaggcgggc atcccgatgc tgcccgtcgc ctacggcgtc gagttcacgc gcctgcacat
     3121 cctgctgtac acgatcctgc tgttcatcgt gacgctgctg ccgtacctga cgggcatgag
     3181 cggcctgctg tacctggcgg cggcgatcgt cctgaacgtg atcttcacgc gccacgctgt
     3241 ccggctgcgc cgtcccgacg cccccgtgca gctgccgatg caggtgttcc gttactcgat
     3301 caatt
//
LOCUS       DASPFR010000354         5757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_715607, whole genome shotgun sequence.
ACCESSION   DASPFR010000354 DASPFR010000000
VERSION     DASPFR010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715607"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1335)
                     /gene="ftsZ"
                     /locus_tag="VD737_09335"
     CDS             complement(160..1335)
                     /gene="ftsZ"
                     /locus_tag="VD737_09335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HXW10807.1"
                     /translation="MFVLADTENQGAIIKVVGVGGGGGNAVAHMVTSGIEGVDFICIN
                     TDAQALKHSKVKTSLQIGSNITKGLGAGADPEVGRQAAMEDRDRIVELIEGSDMIFIT
                     AGMGGGTGTGAAPIVAQVAKELGILTVAVVTKPFEMEGNKRNSLAEQGINELHKYVDS
                     LITIPNQKLLTVLGGDITLLDAFKSANQVLQGAVQGIAELITRPGMINVDFADVKTVM
                     AETGMAMMGSGHAKGENRARAAAESAISSPLLEDVNLSGAHGLLINVTAGMDLRTSEF
                     HTVGETVKQFASEDATVVVGYVIDHEMSDEIRVTVVATGIGRAQPKTMQKPQVEVVTG
                     GRGRRPTAGPFGHNYADLEGPAYTRRTQRAVGDGVIPTDLGEESYLDIPAFLRRQAD"
     gene            complement(1452..2684)
                     /gene="ftsA"
                     /locus_tag="VD737_09340"
     CDS             complement(1452..2684)
                     /gene="ftsA"
                     /locus_tag="VD737_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HXW10808.1"
                     /translation="MPRKPERNLIVGLDIGTSKVVAIVGELGHDGTIDVVGVGSHPSR
                     GLKRGVVVNIDSTVQSIQRAVEEAELMAGCQIHSVFAGIAGSHVRSLNSHGIVAIRDK
                     EVMPGDVERVIDAARAVAIPADQKILHILPQEFIIDAQDGIREPVGMSGVRLEAKVHI
                     VTGAESAAQNIVKCVQRCGLEVEDIVLEQLASSYAVLAEDEKELGVCLIDIGGGTTDI
                     AVFAGGAIRHTAVIPIAGDMVTNDIAQSLRTPTHHAEELKVKYACALSQLANADETIE
                     VPSVGDRPARRLARQTLASVVEARYEEVFVLVRDELARSDYQDKIAAGIVLTGGSAKM
                     DGVIELAEEIFHAPVRLGLPQGVRGLVDVVRNPIHSTGVGLLLFGRSTRHLNPAPRSG
                     VSARRLIDRMRDGFNGNF"
     gene            complement(2677..3480)
                     /locus_tag="VD737_09345"
     CDS             complement(2677..3480)
                     /locus_tag="VD737_09345"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HXW10809.1"
                     /translation="MIAWPFFGRKPRRQATRTEPHWYEKLDWHALGTWAAVAATCVVA
                     VLLLIFAIDRPVRRVLVEGSFQRVAPPDVETAVTQAARGGLASVDLARIRARVEAIDW
                     VDRAVVTRVWPDAIRVVVGEQVAAARWNDTGLLNARGELFIRKTRYMPPELPLLEGPE
                     GSEGDVAQLYIEAQGRLLEVGLRLTGVRLDERGAWELRLGDGLQVRLGRQDVHERLER
                     FIRLASPMVAKRVGEISYVDMRYTNGFSVGWNARSALAVAPNEDATPDA"
     gene            complement(3477..4451)
                     /locus_tag="VD737_09350"
     CDS             complement(3477..4451)
                     /locus_tag="VD737_09350"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HXW10810.1"
                     /translation="MTSRPLTSDPRDFGRVAVLLGGTSSEREVSLDSGRNVLEALQRR
                     GVDAHAVDGIPALVQALTSGNGPRYDRVFNILHGNRGGGEDGVLQGLLEALGLPYTGS
                     GVLGSALSMDKIRTKQVWLSVGLPTPGYLSLQRGEDVVAAAHKLGLPVIVKPACEGSS
                     VGITRCFKDDDLAAAVELAARYEGPLLVEQLIQGDEFTVGVLGAEALPSIRIVPAGEY
                     YDYHAKYVAEDTQYLCPGLAGAAEQELQALALAAFFAVGCSGWGRVDFMRDRAGKPWL
                     LEVNTAPGMTSHSLVPKAARQRGLDFDEVCWRVLETSLHGRAPGEAAR"
     gene            complement(4448..>5757)
                     /gene="murC"
                     /locus_tag="VD737_09355"
     CDS             complement(4448..>5757)
                     /gene="murC"
                     /locus_tag="VD737_09355"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HXW10811.1"
                     /translation="RHGDVTRRLESLGARIAIGHAAANVEGADVVVVSSAINVENPEV
                     AYALERRIPVVKRAEMLGELMRFRYSIAVAGTHGKTTTTSLIASVLAEGGTDPTFVIG
                     GRLKSAASNARLGAGEYLVAEADESDASFTHLQPMLAIVTNVDADHLGTHGGDFEKLK
                     ASFVEFLHNLPFYGLAVICSDDANARSLIPQISRPYLTYGFDADADVRAVDVERLPGR
                     SRFTVLRPRGAPPLAVTLNLPGRHNILNALAAITIATELEVDDAAIQRALAGFQGIER
                     RLQWLGDLETGAGKLTLVDDYGHHPTEIAATVEAARQGWPGRRIVLVFQPHRYTRTRD
                     LFDEFAQVLADADALVVAEVYPAGEAPIPGADGKALCRAIRTRGKVEPVLLRSLEEMP
                     DALAGIVRDGDVVLTMGAGQIGNASHELPRSLPAALAARGPRP"
BASE COUNT          811 a         2051 c         1983 g          912 t
ORIGIN      
        1 aatgctttgg gcgctcccac attcatcgcc ggaccgaaaa ctggagtgtc ccctgtcctc
       61 gaaagcgaaa gcgacgcggg accagcgggg cgcccggagc agagcgcccg cgctggtccc
      121 gcacgcctcc ctcgtggctg aaacgaggga ggccctcgat cagtcggcct ggcgccgcag
      181 gaacgccggg atgtccaggt acgactcttc gcccaggtcg gtcgggatca cgccgtcgcc
      241 gaccgcacgc tgcgtacgcc gcgtgtacgc cggtccctcg aggtcggcgt agttgtggcc
      301 gaacgggccc gcggtgggac gtcgaccgcg accgcccgtc acgacctcga cctgcggctt
      361 ctgcatcgtc ttgggctgcg cccggccgat cccggtcgcc acgacggtca cgcgaatctc
      421 gtccgacatc tcgtggtcga tcacgtaacc caccacgacg gtcgcgtctt ccgaagcgaa
      481 ctgcttgacc gtctcgccga ccgtgtggaa ctcgctggtg cgcaggtcca tgccggccgt
      541 cacgttgatc agcaggccgt gcgcgccgga caggttcacg tcctcgagca gcgggctcga
      601 gatcgcggac tcggcggcgg cgcgggcgcg gttctcgccc ttggcgtgac ccgatcccat
      661 catcgccatg ccggtctcgg ccatcacggt cttcacgtcg gcaaagtcga cgttgatcat
      721 gcccgggcgg gtgatcagtt cggcgatgcc ctgcaccgcg ccctgcagca cctggttggc
      781 gctcttgaac gcgtcgagca gcgtgatgtc gccgccgagc acggtcagca gcttctggtt
      841 cgggatcgtg atcagcgagt cgacgtactt gtgcagctcg ttgatgccct gctcggcgag
      901 cgagttgcgc ttgttgcctt ccatctcgaa cggcttggtg acgaccgcta ccgtcaggat
      961 gccgagctcc ttcgcgacct gcgcgacgat cggcgccgcg cccgtgccgg tgccgccgcc
     1021 catgcccgcg gtgatgaaga tcatgtccga gccttcgatc agctcgacga tgcggtcgcg
     1081 gtcttccatc gcggcctggc gaccgacctc gggatcggcg ccggcgccga ggcccttggt
     1141 gatgttcgag ccgatctgca ggctcgtctt gaccttcgag tgcttgagcg cctgcgcgtc
     1201 ggtgttgatg cagatgaagt cgacgccctc tatacctgag gtgaccatgt gcgcgacggc
     1261 gttgccgcca ccgccgccca cgccgacgac cttgatgatt gccccttggt tttccgtgtc
     1321 tgccagtacg aacattgttt gctgctccgt aggtaaagaa tgcaagcccg ctcagccaac
     1381 ctcacgaagg gcatccagcc cttcccttcc catgcactag ccacgagccg ctggccactg
     1441 gccaccggcc gctaaaaatt tccgttgaac ccatcccgca tccggtcgat cagccggcgc
     1501 gcgctcacgc cgctgcgtgg cgccggattc aggtgccgcg tgctgcgccc gaacagcagc
     1561 agcccgacgc cggtcgagtg aatcgggttc cgcacgacgt cgaccagccc gcgcacgccc
     1621 tgcggcagtc cgagccgcac cggcgcgtgg aagatctcct cggcgagttc gatgacgccg
     1681 tccatcttgg cgctgccgcc ggtcagcacg atcccggccg cgatcttgtc ctggtagtcc
     1741 gagcgcgcga gctcgtcgcg caccagcacg aagacttcct cgtagcgcgc ctcgacgacg
     1801 ctcgcgagcg tctgccgcgc gagccgtcgc gccgggcgat cgccgacgct cggcacctcg
     1861 atcgtctcgt cggcgttggc gagctgcgac agcgcgcagg cgtacttgac cttgagttcc
     1921 tcggcgtggt gcgtcggcgt gcgcagcgac tgcgcgatgt cgttggtgac catgtcgccg
     1981 gcgatcggga tgaccgccgt gtggcggatc gcaccgcccg cgaacaccgc gatgtcggtc
     2041 gtgccgccgc cgatgtcgat caggcagacg ccgagttcct tctcgtcctc ggcgagcacc
     2101 gcgtagctcg aggcgagctg ctcgagcacg atgtcctcga cctcgagccc gcagcgctgc
     2161 acgcacttga cgatgttctg cgcggcgctc tcggcgcccg tcacgatgtg gaccttcgcc
     2221 tcgaggcgca cgccggacat tccgaccggc tcgcggatgc cgtcctgcgc gtcgatgatg
     2281 aattcctggg gcaggatgtg caggatcttc tggtccgccg ggatcgccac cgcgcgggcc
     2341 gcgtcgatga cgcgctcgac gtcgcccggc atgacttcct tgtcgcggat cgcgacgatg
     2401 ccgtgcgagt tcaggctgcg gacgtgactg cccgcgatgc cggcgaacac cgagtggatc
     2461 tggcagccgg ccatcagctc ggcctcctcg accgcacgct ggatcgactg caccgtggaa
     2521 tcgatgttga ccacgacgcc gcgcttgagc ccgcgcgacg ggtgcgaccc cacgccgacc
     2581 acgtcgatcg tgccgtcgtg cccgagttcg cccacgatcg cgaccacctt cgaggtgccg
     2641 atgtcgagcc cgacgatcag gttgcgttcg ggtttcctag gcatcggggg tcgcatcctc
     2701 gttcggcgcg acggcgagcg cgctgcgggc gttccacccg acactgaaac cgttggtgta
     2761 gcgcatgtcc acgtagctga tctcgccgac gcgcttcgcc accatcgggc tcgcgagccg
     2821 tatgaagcgc tcgaggcgct cgtgcacgtc ctggcggccg agcctgacct gcaggccgtc
     2881 gccgagacgc agctcccagg cgccgcgctc gtcgagccgc acgccggtca ggcgcagccc
     2941 cacttcgagc agccggccct gtgcctcgat gtagagctgc gcgacgtcgc cctcgctgcc
     3001 ctcgggccct tcgagcagcg gcagctcggg cggcatgtag cgcgtcttgc ggatgaagag
     3061 ttcgccgcgc gcgttgagca ggccggtgtc gttccagcgg gccgcggcga cctgctcgcc
     3121 caccacgacg cggatggcgt cgggccacac gcgcgtgacg accgcgcgat cgacccagtc
     3181 gatcgcctcg acgcgcgcac ggatcctcgc gaggtccacc gaggcgaggc caccacgggc
     3241 ggcctgcgtc acggccgtct cgacgtccgg cggcgcgacg cgctggaacg agccctcgac
     3301 caggacgcgc cgcaccgggc ggtcgatcgc gaagatcagc agcagcacgg cgacgacgca
     3361 ggtggccgcg accgccgccc aggtgccgag cgcgtgccag tcgagctttt cgtaccagtg
     3421 cggctcggtg cgagtcgcct ggcgacgcgg cttcctgccg aagaagggcc aggcgatcac
     3481 cgcgcggcct cccccggggc gcgcccgtgc aggctggtct cgagcacgcg ccagcagact
     3541 tcgtcgaagt cgaggccgcg ctgccgcgct gccttcggca cgagcgagtg actggtcatg
     3601 ccgggcgccg tgttgacctc gagcagccag ggcttgccgg cgcggtcgcg catgaagtcg
     3661 acgcgacccc agccgctgca gccgaccgcg aagaacgccg ccagcgcgag cgcctgcagt
     3721 tcctgctccg cggcgccggc gagccccggg cagaggtact gggtgtcctc ggcgacgtac
     3781 ttcgcgtgat agtcgtagta ctcgcccgcc ggcacgatcc ggatcgacgg cagggcctcg
     3841 gcgccgagca cgccgacggt gaactcgtcg ccctggatca gctgctcgac cagcagtggc
     3901 ccttcgtagc gcgcggcgag ttcgaccgcc gcggccaggt cgtcgtcctt gaagcagcgc
     3961 gtgatgccga cgctcgaccc ctcgcaggcg ggcttgacga tgaccggcag gccgagcttg
     4021 tgcgcggccg ccacgacgtc ctcgccgcgc tgcaggctca ggtacccggg cgtcggcagg
     4081 ccgaccgaga gccacacctg cttggtgcgg atcttgtcca tcgacagtgc cgagccgagc
     4141 acgccggagc cggtgtacgg cagcccaagc gcctcgagca ggccctgcag cacgccgtcc
     4201 tcgccgccgc cgcggttgcc gtgcaggatg ttgaagacgc ggtcgtaccg cggcccgttg
     4261 cccgacgtga gtgcctgcac cagcgccggg atgccgtcga ccgcatgcgc gtcgacgccg
     4321 cgacgctgca gtgcctcgag cacgttgcgc ccggaatcga gcgacacctc gcgctcggag
     4381 gacgtgccgc cgagcagcac ggcgacgcga ccgaaatcgc gtgggtcgga ggtgaggggt
     4441 cgtgacgtca tggcctcggg cctcgcgcgg ccagggcggc cggcagtgaa cgcggcagct
     4501 cgtgcgaggc gttgccgatc tgccccgcac ccatggtcag cacgacgtcg ccgtcgcgaa
     4561 cgatgccggc gagcgcgtcg ggcatctcct cgagcgacct gagcagtacc ggctcgacct
     4621 tgccgcgcgt gcggatcgca cgacagagcg ccttgccgtc ggcgccgggg atcggcgcct
     4681 cgccggccgg gtagacctcg gcgacgacca gcgcgtcggc atcggcgagc acctgcgcga
     4741 attcgtcgaa caggtcgcgc gtgcgcgtgt agcggtgcgg ctggaacacc agcacgatgc
     4801 gccgtcccgg ccagccctgg cgtgccgcct cgaccgtcgc cgcgatctcg gtcgggtggt
     4861 ggccgtagtc gtcgaccagc gtgagcttcc cggcgcccgt ctccaggtcg ccgagccact
     4921 gcaggcgacg ttcgatgccc tggaagccgg cgagcgcgcg ctggatcgcc gcgtcgtcca
     4981 cctcgagctc ggtcgcgatc gtgatcgcgg cgagcgcatt caggatgttg tggcggcccg
     5041 gcaggttcag cgtgacggcg agcggcggcg cgccacgcgg ccgcaggacc gtgaaccgcg
     5101 accggcccgg caggcgctcg acgtcgacgg cgcgcacgtc cgcgtcggcg tcgaagccgt
     5161 aggtcaggta cgggcgcgag atctgcggga tcaggctgcg ggcgttcgcg tcgtcgctgc
     5221 agatcacggc gagcccgtag aacggcaggt tgtgcaggaa ctcgacgaag ctggccttga
     5281 gcttctcgaa gtcaccgccg tgcgtgccga ggtggtcggc gtcgacgttg gtgacgatcg
     5341 cgagcatggg ctgcaggtgc gtgaacgacg cgtcgctctc gtcggcctcg gcgaccaggt
     5401 actcgccggc accgagcctc gcgttgctcg ccgcgctctt caggcgaccg ccgatcacga
     5461 acgtcgggtc ggtgccgcct tcggcgagaa cgctggcgat caggctcgtg gtcgtggtct
     5521 tgccgtgcgt gccggccacc gcgatcgagt agcggaagcg catcagctcg ccgagcattt
     5581 ccgcacgctt gacgacgggg atgcgccgct cgagcgcgta ggcgacctcc gggttctcga
     5641 cattgatcgc gctcgagacg accacgacgt ccgcgccctc gacgttcgcg gcggcgtggc
     5701 cgatcgcaat gcgggcgccc agcgactcga gccgccgggt gacgtccccg tgccgca
//
LOCUS       DASPFR010000355        16502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_594532, whole genome shotgun sequence.
ACCESSION   DASPFR010000355 DASPFR010000000
VERSION     DASPFR010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16502
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594532"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..1340)
                     /gene="dnaN"
                     /locus_tag="VD737_09360"
     CDS             complement(240..1340)
                     /gene="dnaN"
                     /locus_tag="VD737_09360"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HXW10812.1"
                     /translation="MKIIAKRESILSPLQAVIGVVERKQTMPILANVLLSAKGGKLSI
                     TATDLEVELVATSDVDVQRGGDITVPGRKLLDICRALPEGTEITMTLEGERMLVRARK
                     SRFTLSTLPAAEFPTVEEINAEQTLELAQKDFRRLLEKTHFSMAQQDVRYYLNGLLLE
                     TSAKMLRAVATDGHRLALSEIQLPEGGKPGQQVIVPRKGVLELQRILGNEDQKLSVSI
                     GSNHLRVQIGEIRFTSKLIDGRFPEYGRVIPNNPPRVVGAVRDDLRQALQRAAILSNE
                     KYRGIRFALKPSALVIQAHNPEQEEAEEEVEVTYEGEELEIGFNVNYLLDALAAVDGT
                     DVQVGLTDGNSSCLIRSGQDGSSRYVVMPMRL"
     gene            complement(1547..2959)
                     /gene="dnaA"
                     /locus_tag="VD737_09365"
     CDS             complement(1547..2959)
                     /gene="dnaA"
                     /locus_tag="VD737_09365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HXW10813.1"
                     /translation="MEISLWHQCLRRLEAELPEQAFNTWIRPLQAVEDGGRLRLLAPN
                     RYVVDWVNQNCVSRIGELVDELVPAPAPSVVVEIGSRRLLPTPAPVLDGVGIPVQPRS
                     AAPRTLPGVAAAMPGDVAGRRPVDPLPQFGGRLNPDFTFSNFVEGKSNQLARAAAMQV
                     AENPGRAYNPLFIYGGVGLGKTHLMHAVGNQIRSRNKDARIAYVHSERFVGDMVRALQ
                     HNTINEFKQAYRTLDALLIDDIQFFAGKERSQEEFFHTFNALLEGQQQVILTCDRYPK
                     EVEGLEERLKSRFGWGLTVAIEPPELETCVAILMSKASQGGVELPEEVAFFVAKRIRS
                     NVRELEGAMRRVIANSQFTGRPITLDFAKEALKDLLALQERLVTIENIQKTVAEYYKI
                     RVADLLSRRRSRSITRPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRVVKEL
                     RDSQQRLNEDYSNLLRTLAG"
     gene            3152..3286
                     /gene="rpmH"
                     /locus_tag="VD737_09370"
     CDS             3152..3286
                     /gene="rpmH"
                     /locus_tag="VD737_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HXW10814.1"
                     /translation="MKRPFQPSNLHRKRVHGFRARMATKGGRLVLSRRRAKGRKRLAV
                     "
     gene            3283..3678
                     /gene="rnpA"
                     /locus_tag="VD737_09375"
     CDS             3283..3678
                     /gene="rnpA"
                     /locus_tag="VD737_09375"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HXW10815.1"
                     /translation="MTVVSTGPGETPSASRRFPRASRLTDKPQFDAVHTRGQRHSDAL
                     FSVITRANDVGRARLGLAVGVKAAGNAAQRNRLKRLTRESFRHRQQELPAVDVVVNAR
                     PGAARSDNAAIRASLAAHWDRIVRRCARS"
     gene            3663..3908
                     /gene="yidD"
                     /locus_tag="VD737_09380"
     CDS             3663..3908
                     /gene="yidD"
                     /locus_tag="VD737_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HXW10816.1"
                     /translation="MRALLIGLINLYRWFVSPLLGPNCRFHPTCSCYAQDAIRLHGAL
                     RGSWLAARRIGRCHPWNPGGYDPVPDARGTSTPATTN"
     gene            3925..5616
                     /gene="yidC"
                     /locus_tag="VD737_09385"
     CDS             3925..5616
                     /gene="yidC"
                     /locus_tag="VD737_09385"
                     /inference="COORDINATES: protein motif:HMM:NF002352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HXW10817.1"
                     /translation="MDNPRIYLWIGLALLVWMNIIQWDRDYGQRAPTPTTAPASVQGS
                     TTPEAPAASQLPSLPSSDPTAPPVAATAPADAAPAATVDTGAPKVRVVTDVLDMDVSL
                     QGGDLLRAELPQYPKDKRPGSPPVRLLTTDEATFGIVRSGLRAADGRAEPTHLVTYTT
                     PNSEYRLRPGAQELRVPLTWTDGQGVTVTKTYVFEPGRYDIDVVYDVQNASSTDWKAA
                     SYVQFVRHVYPQERSMFDVESYAFRGPAFYDGGKYRKLDVEDEDDLALRTSFTNGWMA
                     EMQHHFVSAAVPPKDQTYDFALQKEQGHSLVSYRGPLRSVPAGESARFTETLFVGPKL
                     QDQLRTVGPKLELTVDYGKLTILADPLFWLLQQVHRFVQNWGVAIIVVTILLKLLFYK
                     LTETSGRSMAKMRTIAPRIKSIQERYKDDREQLAKQMMELYKREKINPVAGCLPILVQ
                     IPVFLAFYWVLLESVEMRQAPFFGWIQDLSSKDPFFVLPILMGVAMFGQFKLNPAPPD
                     PMQAKIFALMPIIMTVMMAWFPAGLVLYWLTNTLLSIAQQWNINRVVELEAKKRS"
     gene            5638..6996
                     /gene="mnmE"
                     /locus_tag="VD737_09390"
     CDS             5638..6996
                     /gene="mnmE"
                     /locus_tag="VD737_09390"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019580343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HXW10818.1"
                     /translation="MSARPDTIAAVATAHGRGGVGVVRVSGPGVRSVAQAVLGCVPAP
                     RQAELHRFEDDAGAVIDVGLALYFPAPHSYTGEDVLELHGHGGPVVMDLLLQRLLGLG
                     ARLATAGEFTQRAFLNDKLDLTQAEAIADLIDSGSAQAARAALRSLRGEFSARVHELA
                     GRTLDLRMWIEAAIDFPEEEVDFLADRALGARMEDLRGRFAELAETARQGALLRDGLT
                     VVIAGRPNAGKSSLLNRLAGYDAAIVTEIPGTTRDVLRERIAIDGLPLHVLDTAGLRE
                     AGDAVEAEGVRRAHAEMARADRVLFVVDASDPGSISGLPDDLERLPAAAPRTVLFNKV
                     DRSGEAPRVETQPDGAVRVYLSAATGAGFEGLRRHLKDCIGFEPAAEGALSARTRHID
                     ALRRARTHVEAANELLVRRHAGELVAQELRDAHQALGEITGEVSSDDLLGRIFSSFCI
                     GK"
     gene            7124..8809
                     /locus_tag="VD737_09395"
     CDS             7124..8809
                     /locus_tag="VD737_09395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HXW10819.1"
                     /translation="MKQLSGADSMFLQFEHGNQFMHVASLSIYDPSTAAGGGVRFKDV
                     LRHFATRVAQFPQFRRRLVSLPLSLDRPYWIESPTIDVEFHVRHIALPHPGDWRQLCI
                     QVARLHSRPLDRSKPLWEAYIIEGLHNVQGVPPGSFALYTKLHHAIIDGESGTELMKA
                     LHSLSPEPTEVDETAHSASTVFHKDREPTLVELYSRALVHGVQRVPSIAKFSISTARK
                     VVHLGSEALTSFRREGEGSLLGNLRALLSGDLSALLPRMPPQTRFSGNVSAHRVFEAV
                     SLPLSEMRAIRQQVPRATINDQFLCVVGGALRAYLSDKNELPDTSMVAMVPMTLRGAD
                     KGGDRGNQVGFTLMPVHTEIEDPLERLRAVVESAVKSKRVTDAIGREIARDLMESLPS
                     IVADPLLRNAQVPRIGLVVSNVRGPDVPLYMAGARLVHYVPISIAINGMGLNVTGFSY
                     AGDLWICAVSCREMLPDPAFFADCLRSAFDQMKEAAAREAAFVAAEVRASEDREDVDA
                     IDGEVVRVVRTPRKRKPAKPAAKPATSKRRPRKVARTAARRSRAAGAPADANA"
     gene            8814..9221
                     /locus_tag="VD737_09400"
     CDS             8814..9221
                     /locus_tag="VD737_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HXW10820.1"
                     /translation="MPAELRRALTDLFGDPVDEVIVRERSWFARLHGRARATTRRNTI
                     YLRGSLDEFLSDPELMLHEYFHVLRQWNRGRMNLWGYLAEWSRRGYWDNRYERQARRF
                     VSLRLAAFRRSLVGSAPGFEPTRRFNLLDEPPA"
     gene            9226..11154
                     /gene="mnmG"
                     /locus_tag="VD737_09405"
     CDS             9226..11154
                     /gene="mnmG"
                     /locus_tag="VD737_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004654475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HXW10821.1"
                     /translation="MRYGDKYDVIVVGGGHAGTEAALAAARMGCRTLLVTQSIEGLGQ
                     MSCNPAIGGIGKGHLVKEIDALGGAMARATDRAGIQFRTLNASKGPAVRATRAQADRA
                     LYRKAIRAIVEQQPNLSLFQAEVADLRIEHGCIAGVVTAVGLEFAAPAVVLTVGTFLG
                     GRMHVGLEQSPGGRAGDPPSNRLAARLRELPLRVGRLKTGTPPRIDGRTIDYRGLVVQ
                     PGDEPTPVFSYLGLRDEHPRQVCCHITHTNERTHELIRAGTDRSPMYTGVIEGVGPRY
                     CPSVEDKVVRFADKASHQVFIEPEGLDTHEVYPNGISTSLPFDVQQAFVRSIRGFEQA
                     HITRPWYAIEYDFFDPRDLQPTLETRALPGLYFAGQINGTTGYEEAAAQGLLAGINAA
                     LRVLEREPWWPQRGGAYLGVLVDDLITRGAPEPYRMFTSRAEYRLTLREDNADLRLTP
                     LGRELRLVDDERWRLFERKRDAVARERARLEAAWVRPGTAPADHLEQTLGLPVVREQR
                     AFEVLRRPEVPYAALVAALGSGADDWREDERLAVQVPLQVDVQAKYTGYIDRQRDEIE
                     RQRRHEQTALPDTLDYASVRGLSNEVRQKLAEHRPSTLGQAARIPGVTPAAISILLIH
                     LKRHHSETPGAARVGGVG"
     gene            11194..12882
                     /locus_tag="VD737_09410"
     CDS             11194..12882
                     /locus_tag="VD737_09410"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HXW10822.1"
                     /translation="MHEPILMPRPSRGGAPRARRAWVLATAACLGLVACGGGDDPTGG
                     TSGGTAAPNAGYDPTTLRRGNGPEPDTLDPQLARTDAAFNILRDLFEGLTAVGADGSP
                     APAAAESWTVSPDGLEYRFALREQLRWSNGDPVVAEDYVAGMRRLVDPATASPYAQFI
                     DPVLNAAAIARGEKKPEALGVAAPDPRTVVVRLAAPAPYLLGLLAQPPTFPLHRASLA
                     AHGAEYARPGKLLSNGAFVLEDWVIGSQVIARRNTRYWNDAETRLDRVHYVHVGDAGT
                     ELRQYRAGQLDVTYVVPQQQFQWIRQKLAGELHVAPQLSVYYYGFNLTRPPFKDQPGL
                     RRALSMVIDRDRLVTAVTGVGEAPAYGWVPPGVWNYTPQQFDYASLPYEARVAEARRL
                     YAQAGYTAERPLEVELRYSSGDTHNRIAVAVAAMWQEALGVKTQLYAEEFRALLQSIQ
                     RKDTQVFRSSWVADFNDAYTFAQLLKSDFGINLTGYSNPRYDALLAEAERQADVGRRR
                     ELLQEAERVLLADHPLLPLYFYVNKHLVKPWVRGWTDNVMNVAYSKDLSLQPAG"
     gene            12987..13277
                     /locus_tag="VD737_09415"
     CDS             12987..13277
                     /locus_tag="VD737_09415"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HXW10823.1"
                     /translation="MAWFVVFAVTVFGLWFWREYRVQQRHLESASAPHRMGERYIGQV
                     ITMPQGIANGEGRIKLGRQDWSVRGPDLPAGGKARITGVDGRVLIVDRMPAR"
     gene            complement(13449..14180)
                     /locus_tag="VD737_09420"
     CDS             complement(13449..14180)
                     /locus_tag="VD737_09420"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HXW10824.1"
                     /translation="MKHAQLGLGRAVWVALMSGLAVAGVASAHDTVPPPLISVTGEGE
                     VKAMPDMAYVTLGVEARKPTLAEARTQVTATVERVLALTRELKIDPKFVDSSQLQVQP
                     EYRWNEKDSQRVLLGYVVSRQIEVELRDLERLGPLLERSVSAGVNVLGGARLDSTRRK
                     ELEREALTRAVDDARLDAETLAKAASARLGPVYSLSASTAMPFQPKYMERAAMSAQAD
                     AAEQTYAPGEMKFVANVSAQYEIAR"
     gene            14359..14985
                     /locus_tag="VD737_09425"
     CDS             14359..14985
                     /locus_tag="VD737_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXW10825.1"
                     /translation="MISRSAWVALSLSLAMLGPGLAHAADKARGGKLAYTCHGCHGIE
                     NYKNAFPVYSVPKLGGQHAAYLAVALKEYQDGNRAHATMHAQAATLKPQDVADVSAYL
                     AGVELKSSGRPTGTLPKAGQTCVACHGNDGIGIMPEYPNLAGQHEDYLAMSLRGYKTG
                     QRKNPIMAGMAAPLTEQDIRELARYYASQRPALCATDDIRKAGQCKGP"
     gene            15092..16057
                     /locus_tag="VD737_09430"
     CDS             15092..16057
                     /locus_tag="VD737_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXW10826.1"
                     /translation="MSATSASPTLRHAQPQGDGLYLIDTDYVRPGLAAAHLLVDDGHA
                     AFIDTGPAPAAPRLLAALDEVGLAPEQVDYLFLTHVHLDHAGGAGQMMRALPNARAVL
                     HPRGAPHLVDPAKLIAGSIAVYGETLYRQLYGELLPIPADRVLTTEDGTRLELGRRTF
                     EFIDAPGHARHHHCPIDLDRRDVYAGDNFGICYRDLDTAAGPFMLPTTTPVQFDPEAM
                     HRTIDRLMAYQPRRIVQTHFGPVTDLERLARDLHASVDELVRIARRFADAPDRTQRIR
                     AAMFDYFSARLDEHGYTGDPATRHRLIDEDVNLNTQGLEVWLSKQ"
     gene            16054..>16502
                     /locus_tag="VD737_09435"
     CDS             16054..>16502
                     /locus_tag="VD737_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXW10827.1"
                     /translation="MNNPFTAFRVHRIDGKIVARFDQVTLDDLTPGDVVVRVSYSDIN
                     YKDALAATGASPILRRYPLVGGIDLSGEVVSSADPRFTPGQKVLVTGCGLSETQDGGY
                     AEYARLQGDWVIPLPDGLDERDAMRLGTAGFTAALAVHRMEQNGQAPG"
BASE COUNT         2601 a         5806 c         5517 g         2578 t
ORIGIN      
        1 ctgatcaggt tgcagcgagg cgcgaactcg agctcggccg actcgatgca acgaaaatcg
       61 cggacctcga agcgtgtcag cagcacgatc gtggctgccc gtaacagcgc gccggtctag
      121 cggcgaggat cagcaccgcc cgccttgctc gggagacacc gctcccgaag ctgatgtcat
      181 caggtggttc cacgtggaac atctggtgtc gcgtcgcgac gttccacgtg gaaccagcgt
      241 cagagcctca tcggcatcac gacgtagcgc gacgatccgt cctggcctga gcgaatcagg
      301 caactgctgt tgccatcggt caggccgacc tgcacgtccg tgccgtccac ggccgccaag
      361 gcatccagca ggtaattcac gttgaagccg atctcgagtt cttcgccctc gtaggtgact
      421 tcgacttcct cttcggcctc ttcctgctca ggattgtgcg cctggatcac gagcgcgctc
      481 ggcttgagtg caaaccggat accccggtac ttctcgttgg agaggatcgc ggcacgctgc
      541 aatgcctgcc gcaggtcgtc ccgcacggcg ccgacgactc gcgggggatt gttcgggatg
      601 acgcgcccgt actccgggaa gcgcccatcg atcagcttgg aagtgaagcg aatttcgccg
      661 atctggacgc gaagatgatt cgatccgatc gaaacggaca gcttctggtc ctcattgccg
      721 agaatccgct gcaactcgag cacccccttg cgcggcacga tcacctgctg acctggttta
      781 ccgccctcgg gcagctggat ctccgacaat gccagacgat gtccatcagt cgcgaccgca
      841 cgcagcattt tcgccgaagt ttcgagcagt aagccgttca ggtagtagcg gacgtcctgc
      901 tgggccatcg aaaagtgcgt cttctcgagc aggcgcctga aatccttctg cgccagctcg
      961 agcgtctgtt ccgcgttgat ctcctcgacc gtcgggaatt cggccgcggg cagcgtcgac
     1021 aacgtaaatc gactcttgcg ggcgcgcacc agcatccgct cgccctccag ggtcatcgtg
     1081 atctcggtgc cttcgggcag cgcacggcag atgtcgagaa gcttgcgacc cggcaccgtg
     1141 atgtcgccac cccgctgcac gtcgacgtcg gaagtcgcga cgagctcgac ttcgagatcg
     1201 gtcgcagtga tggagagctt gccccccttg gccgagagca gcacgttcgc gaggatcggc
     1261 atcgtctgct tgcgctcgac cacgccgatc acggcttgca gcgggctcag gatcgattcg
     1321 cgcttcgcga tgatcttcat cttattaatc tcggatcttt atctaagaat tctattgcta
     1381 cgacttttag cacagcccca gtctgtgcaa aacctcaggt tccccaatga cggcgcgcct
     1441 ttgcggcgcc ggtcaacctg gctgtgaaca cctgcggcgc ctgtccgcag cctgtggagg
     1501 aaatgtggag cacttcagcc acattttcgt ccacagcttg ctccactcat cccgcaagcg
     1561 ttctcaacag gttcgagtag tcttcgttga gacgctgctg cgaatcgcgc aactccttca
     1621 cgacacgaca cgcgtgcagc accgtcgtgt ggtcgcgacc gccgaacgcg tcgccgatct
     1681 ccggcaggct gtggttcgtg agttccttcg cgagcgccat cgccatctgt cgcggccgcg
     1741 tgatcgagcg gctgcgcctg cgcgagagca ggtccgcgac gcggatcttg tagtactccg
     1801 cgaccgtctt ctggatgttc tcgatcgtga ccaggcgctc ctgcagcgca agcaggtcct
     1861 tgagcgcttc cttcgcgaaa tccagtgtga tcggacgacc cgtgaactgc gaattggcga
     1921 tcacgcgccg catcgcgcct tcgagttcgc gcacgttcga acggatgcgc ttcgccacga
     1981 agaaagcgac ttcttccggc agctcgacgc cgccctgcga ggccttgctc atcaggatcg
     2041 cgacgcacgt ctcgagctcc ggtggctcga tcgcgaccgt gagcccccag ccgaagcgcg
     2101 acttcagccg ctcctcgagg ccttcgacct ccttcggata tcgatcgcag gtcaggatca
     2161 cctgctgctg gccctcgagc agcgcgttga acgtgtggaa gaactcttcc tgcgaacgct
     2221 ccttgccggc gaagaactgg atgtcgtcga tcagcagcgc gtcgagcgtg cggtacgcct
     2281 gcttgaattc gttgatcgtg ttgtgctgga gcgcgcgcac catgtcgccg acgaaacgct
     2341 cggaatgcac gtaggcgatg cgggcgtcct tgttgcgcga ccgtatctgg ttgccgaccg
     2401 cgtgcatcag gtgcgtcttg ccgaggccga caccgccgta gatgaacaac gggttgtagg
     2461 cgcggcccgg attctcggcg acctgcatcg cggccgcgcg ggcgagctgg ttgctcttgc
     2521 cctcgacgaa gttggagaac gtgaagtccg ggttcaggcg tccgccgaac tgcggcaacg
     2581 gatcgacggg acgcctgccg gcgacgtccc cgggcatcgc cgccgcgaca ccaggcaacg
     2641 tccgtggcgc tgcggagcgc ggctgcaccg ggatcccgac gccgtcgagc acgggcgcag
     2701 gcgtcggcag caggcgcctc gaaccgatct ccaccacgac gcttggcgcg ggcgccggca
     2761 ccagctcgtc gacgagctcg ccgatgcgcg aaacgcagtt ctggttgacc cagtcgacga
     2821 cgtagcgatt cggggcgagc aaccgcagcc gaccgccgtc ctcgacggcc tgcaaaggcc
     2881 ggatccaggt gttgaacgcc tgttcgggga gctcagcctc caggcgacgg aggcactgat
     2941 gccaaaggga aatctccacg accggcggac ctgcgacgtg ctcggaagct cgaacgaacg
     3001 gaaggggcgg ggagtgtata ccgacccctt gcctgtggat atctcgggtt gcggttgacg
     3061 ccccgcggac cggtgcgtat cctttcgccc ccttttacga ccagtgcatg ttgttgtttc
     3121 tggtcttgtt tcgggcacca cggagccgaa catgaagcgc ccctttcaac ccagcaacct
     3181 gcaccgcaag cgcgtccacg gattccgtgc gcgcatggcc acgaagggtg gtcgtctggt
     3241 cctcagccgt cgccgcgcca agggccgcaa gcgcctcgcg gtctgaccgt cgtttccacg
     3301 ggccccggcg agaccccatc cgcctcgcgt cgctttccgc gcgcgagccg cctgaccgac
     3361 aagccgcagt tcgacgccgt ccacacgcgg ggccagcgcc attcagacgc gctgttctcg
     3421 gtgatcacac gcgccaatga cgtgggtcgc gcgcggctcg gcctggcggt cggtgtcaag
     3481 gctgccggca acgccgccca gcgcaatcgg ctgaagcgtt tgacccgcga atcgtttcga
     3541 caccgccagc aggagctgcc agccgtggac gtcgtcgtca acgcgcgccc cggcgccgcc
     3601 cgatccgaca acgcagcgat ccgcgcgagc ctggccgccc actgggatag gatcgtgcga
     3661 cgatgcgcgc gctcctgatc ggcctgatca atctctaccg gtggttcgtg agcccgctgc
     3721 tcgggccgaa ctgccgcttc cacccgacct gctcctgcta cgcgcaggac gcgatccgac
     3781 tgcacggcgc gctgcgcgga tcgtggctgg cggctcgccg catcggccgc tgccacccct
     3841 ggaatcccgg cggttacgac cccgtgcccg atgcccgcgg cacgtccacg cccgcaacaa
     3901 cgaactgaca cgatcgatcg agccatggac aacccgcgca tctacttgtg gatcggcctc
     3961 gccttgctcg tctggatgaa catcatccag tgggaccgcg attacgggca gcgcgcgccg
     4021 acgcccacca ccgcacccgc gagcgtgcag ggctccacga ctcccgaggc gccggccgcg
     4081 agccagctgc catcgctgcc gtccagcgac ccgacggcgc caccggttgc ggccaccgca
     4141 ccggcggacg cggctcccgc cgccacggtc gacaccggcg cgccgaaggt gcgcgtggtc
     4201 acggacgtgc tcgacatgga cgtgagcctg cagggcggtg acctgttgcg ggccgagctg
     4261 ccacagtatc caaaggacaa gcggcccggc agcccgccgg tgcgcctgct gaccaccgac
     4321 gaagcgacgt tcggcatcgt ccgcagtgga ctgcgcgccg ccgacggccg cgccgagccc
     4381 acgcacctcg tgacctacac gacgccgaat tcggaatatc gactccggcc cggcgcgcag
     4441 gagctgcgcg tgccgctcac ctggaccgac gggcagggcg tcacggtcac caagacctac
     4501 gtcttcgagc cggggcgcta cgacatcgac gtcgtctacg acgtgcagaa cgcttcgtcg
     4561 accgactgga aagcggcgtc gtacgtgcag ttcgtgcgac acgtctatcc gcaggagcgc
     4621 tcgatgttcg acgtcgagag ctacgcgttc cgcgggcccg cgttctacga cggcggcaag
     4681 taccgcaagc tcgatgtcga agacgaggac gacctggcgt tgcggacgtc gttcaccaat
     4741 gggtggatgg ccgagatgca gcaccacttc gtctcggccg cggtgccgcc gaaggaccag
     4801 acctacgact tcgcgctgca gaaggagcag ggccactcgc tggtgagcta tcgcggcccg
     4861 ctgcggagcg tgccggccgg cgagagtgcc cggttcaccg agacgctgtt cgtgggcccg
     4921 aagctgcagg accagctgcg cacggtcggc ccgaagctcg agctcacggt cgactacggc
     4981 aagctgacga tcctcgccga cccgctgttc tggctgctgc agcaggtgca ccgcttcgtg
     5041 cagaactggg gcgtggcgat catcgtcgtc acgatcctgc tcaagctgct gttctacaag
     5101 ctcaccgaga cgagcggccg ctcgatggca aagatgcgca cgatcgcccc gcgcatcaag
     5161 agcatccagg agcgctacaa ggacgaccgt gagcagctcg ccaagcagat gatggagctg
     5221 tacaagcgcg agaagatcaa cccggtcgcg ggctgcctgc cgatcctggt gcagatcccc
     5281 gtgttcctgg cgttctactg ggtgctgctc gagagcgtcg agatgcgcca ggcgccgttc
     5341 ttcggctgga tccaggacct ctcgtccaag gacccgttct tcgtcctgcc gatcctgatg
     5401 ggcgtggcga tgttcgggca gttcaagctg aacccggcgc cgcccgaccc gatgcaggcg
     5461 aagatcttcg cgctgatgcc catcatcatg accgtgatga tggcctggtt ccccgcgggc
     5521 ctggtgctct actggctcac caacacgctg ctgtcgatcg cgcagcagtg gaacatcaac
     5581 cgggtcgtcg agcttgaggc gaagaaacgg agctgagcgc ccgtcggcgc cggcgcgatg
     5641 agcgcgcgcc ccgacacgat cgcggccgtc gcgacggcgc atggccgcgg cggcgtcggc
     5701 gtcgtgaggg tctcggggcc cggggtgcgc tcggtcgccc aggcggtgct cggttgcgtg
     5761 cccgcgccgc gccaggccga gttgcaccgc ttcgaggacg acgcgggtgc ggtgatcgac
     5821 gtcgggctcg cgctctactt tccggccccg cactcgtaca ccggcgagga cgtgctcgaa
     5881 ctgcacgggc acggcggacc ggtcgtcatg gacctgctgc tccagcgcct gctcggcctg
     5941 ggcgcgcggc ttgcgacggc gggcgagttc acgcagcgcg cgttcctgaa cgacaagctc
     6001 gacctgacgc aggccgaggc gatcgccgac ctgatcgaca gcggttccgc ccaggccgcg
     6061 cgagcggcgc tgcgctcgct gcggggcgag ttctcggcgc gcgtgcacga actggccggg
     6121 cgcacgctcg acctgcgcat gtggatcgag gcggcgatcg atttcccgga ggaggaggtc
     6181 gacttcctgg ccgaccgcgc cctcggcgcc cgcatggagg acctgcgggg acgcttcgcg
     6241 gagctggccg agaccgcgcg ccagggcgcg ttgctccgtg acgggctcac cgtcgtgatc
     6301 gccggacgtc ccaacgccgg caagtcgagc ctgctcaacc gcctggccgg ctacgacgcc
     6361 gcgatcgtca ccgagatccc gggcaccacg cgcgacgtgc tgcgcgaacg catcgcgatc
     6421 gacggcctgc cgctgcacgt tctcgacacg gcgggattgc gcgaggcggg cgacgcggtc
     6481 gaggccgaag gcgtgcgtcg cgcgcacgcc gagatggccc gggcggaccg cgtgctgttc
     6541 gtggtcgacg cgtcggatcc cgggtcgatt tccggcctgc ccgacgacct cgagcgcctg
     6601 ccggcggcgg cgccgcgcac cgtcctgttc aacaaggtcg accgcagcgg cgaggcgccg
     6661 cgggtcgaga cccagccgga cggcgccgtc cgggtgtacc tctcggccgc gaccggcgcg
     6721 ggcttcgagg gcctgcgacg gcacctcaag gactgcatcg ggttcgagcc tgccgccgag
     6781 ggcgccctct cggcacggac ccggcacatc gacgccttgc ggcgcgcgcg gacgcacgtc
     6841 gaagcggcca atgagctgct ggtccgtcgc cacgccggcg aactcgtggc gcaggagctg
     6901 cgcgacgccc accaggcgct gggcgagatc actggcgagg tgtcgagcga cgacctgctg
     6961 ggcaggatct tttcgagctt ctgcatcggc aagtagccgg ttcgccgcac cccgtggcga
     7021 ctggcggtaa agcgctctcc aatgagcccc gttggcgcct ccgcctccgt tatgattgtc
     7081 gacccgccgc gcggcgtccg cggccatcga cggatctaga gccatgaagc agctttccgg
     7141 cgcggattcg atgttcctgc agttcgagca cggcaatcag ttcatgcacg tggcctcgct
     7201 gtcgatctac gacccgtcga cggctgccgg cggcggcgtg cgcttcaagg acgtgctgcg
     7261 ccacttcgcg acccgggtgg cgcagttccc gcagttccgg cgcaggctcg tctcgctgcc
     7321 gctgtcgctc gaccgcccgt actggatcga atcgccgacg atcgacgtcg aattccacgt
     7381 ccggcacatc gcgctgcccc accccggcga ctggcgccag ctctgcatcc aggtcgcgcg
     7441 actgcattcg cgaccgctcg accgcagcaa gccgctctgg gaggcgtaca tcatcgaagg
     7501 cctgcacaac gtgcagggcg tgccgcccgg cagcttcgcg ctctacacca agctgcacca
     7561 cgccatcatc gacggtgagt cgggcaccga gctcatgaag gcgctgcact ccctgtcgcc
     7621 ggagccgacc gaggtcgacg agacggctca cagcgcctcg accgtgttcc acaaggatcg
     7681 ggaacctacg ctggtcgagc tgtattcgcg ggcgctggtg cacggcgtgc agcgcgtgcc
     7741 gtcgatcgcg aagttctcga tttccaccgc ccgcaaggtc gtccacctcg gcagcgaggc
     7801 gctcacgagc ttccggcgcg aaggcgaggg cagcctgctc ggcaaccttc gcgcgctgct
     7861 gtcgggcgac ctgagcgcgc tcttgccgcg catgccgccg cagacccgct tcagcggcaa
     7921 cgtctcggcg catcgcgtgt tcgaggccgt gtcgctgccg ttgtcggaga tgcgcgcgat
     7981 ccgccagcag gtgccgcgcg cgaccatcaa tgatcagttc ctgtgcgtgg tcggcggtgc
     8041 gctgcgcgcg tacctgtccg acaagaacga gctgcccgac acgtcgatgg tcgcgatggt
     8101 gccgatgacg ctgcgcggcg ccgacaaggg cggcgaccgt ggcaaccagg tcggcttcac
     8161 gctgatgccg gtgcacaccg agatcgagga cccgctcgag cgcctgcgcg cggtggtcga
     8221 gtccgccgtc aagtcgaagc gcgtcaccga cgcgatcggc cgcgagatcg cgcgcgacct
     8281 gatggagtcg ctgccgagca tcgtcgccga tccgttgctg cgcaacgcgc aggtgccgcg
     8341 catcggcctg gtcgtctcga acgtgcgcgg gccggacgtg ccgctctaca tggcgggcgc
     8401 ccggctggtg cactacgtgc cgatcagcat cgcgatcaac ggcatgggcc tcaacgtcac
     8461 cggtttcagc tatgcaggcg acctgtggat ctgcgcggtg tcctgccgcg agatgctgcc
     8521 cgacccggcg ttctttgccg attgcctgcg ctccgcgttc gaccagatga aggaagccgc
     8581 cgcccgtgaa gcggccttcg tggcggccga ggtccgggcg agcgaagacc gcgaggacgt
     8641 cgacgccatc gacggcgaag tggtccgcgt cgtgcggacg ccgcgcaagc gcaagcccgc
     8701 caagccggct gccaagccgg cgacgtcgaa gcgtcgcccg cgcaaggtcg cgaggaccgc
     8761 tgcccgccgc tcgcgcgccg ccggcgcgcc ggccgatgcg aacgcgtgac tgcgtgcccg
     8821 ccgagttgcg gcgggcgctg acggacctct tcggcgaccc ggtggacgag gtcatcgtgc
     8881 gcgagcgctc atggtttgcc cggttgcacg ggcgggcacg cgccaccacc cggcgcaaca
     8941 cgatctacct gcgtggttcg ctcgacgagt tcctgagcga cccggagctg atgctgcacg
     9001 agtatttcca cgtgctgcgg cagtggaacc gcgggcgcat gaacctctgg ggctacctgg
     9061 cggaatggag ccgccgcgga tactgggaca accgttacga gcggcaggcg cggcggttcg
     9121 tgagcctgcg gctcgcggcg ttccgccggt cgctggtagg ctccgcgccc ggattcgagc
     9181 cgacccgacg cttcaacctg ctggacgagc ccccggcctg acctcatgcg ctacggagac
     9241 aagtacgacg tgatcgtggt gggcggtggc cacgccggca ccgaagctgc gctcgccgcg
     9301 gcccgcatgg gctgtcgcac gctgctcgtg acgcagagca tcgaggggct cggccagatg
     9361 agctgcaacc cggcgatcgg cggcatcggc aagggacacc tcgtcaagga gatcgacgcg
     9421 ctcggcggtg cgatggcccg ggcgaccgat cgcgccggca tccagttccg cacgctcaac
     9481 gcgagcaagg gcccggccgt gcgggcgacg cgcgcccagg ccgatcgtgc gctctatcgc
     9541 aaggcgattc gcgcgatcgt cgagcagcag ccgaacctgt cgctgttcca ggccgaggtc
     9601 gccgacctgc gcatcgagca cgggtgcatc gccggtgtcg tgacggccgt cggcctcgag
     9661 ttcgccgcgc ccgcggtcgt gctcaccgtg ggcacgttcc tgggcggccg catgcacgtg
     9721 gggctcgagc agtcgcccgg cggacgcgcc ggcgacccgc cttccaatcg tctcgcggcg
     9781 cgtctgcggg aactgcccct gcgcgtgggc cggctcaaga ccgggacgcc gccgcgcatc
     9841 gacgggcgca cgatcgacta ccggggtctc gtcgtgcagc cgggcgacga gccgacgccg
     9901 gtgttctcat acctgggcct gcgcgacgag cacccgcgcc aggtctgctg ccacatcacg
     9961 cacaccaacg agcgcacgca cgagctgatc cgcgcgggca ccgaccgttc gccgatgtac
    10021 accggcgtga tcgagggcgt cgggccccgc tactgcccgt cggtcgagga caaggtcgtg
    10081 cgcttcgccg acaaggcgtc gcaccaggtg ttcatcgaac ccgagggcct cgacacgcac
    10141 gaggtctacc cgaacggcat ctcgaccagc ctgccgttcg acgtgcagca ggcgttcgtg
    10201 cgcagcatcc gcggcttcga gcaggcgcac atcacacggc cctggtacgc aatcgaatac
    10261 gacttcttcg atccgcgcga cctgcagccg acgctcgaga cccgggcgct gccgggcctc
    10321 tacttcgccg ggcagatcaa cggcacgact ggttacgagg aagccgccgc gcagggcctg
    10381 ctcgccggga tcaacgcggc cctgagggtc ctggagcgcg agccgtggtg gccgcagcgg
    10441 ggaggcgcct acctcggcgt gctggtcgac gacctgatca cgcgaggcgc gcccgagccg
    10501 taccggatgt tcacgagtcg cgcggagtac cggctgacgt tgcgcgaaga caatgccgac
    10561 ttgcgactca cgccactcgg gcgcgaactt cgtctcgtcg acgacgagcg ctggcgcttg
    10621 ttcgagcgca agcgcgacgc cgtcgcccgt gaacgcgccc ggctcgaggc ggcatgggta
    10681 cggcccggca cggcgccggc cgatcatctc gagcagacgc tcgggctgcc ggtggtgcgc
    10741 gagcagcggg cgttcgaagt gctgcgccgg ccggaggtcc cgtacgcggc cctcgttgcc
    10801 gcgctcggca gcggtgccga cgactggcgc gaggacgagc gcctcgccgt gcaggtgccc
    10861 ctgcaggtcg acgtgcaggc gaagtacacc ggctacatcg accgccagcg cgatgaaatc
    10921 gagcggcagc gccgtcacga gcagacagcg ctgcccgaca cgctcgatta cgcgagcgtg
    10981 cgcgggctgt cgaacgaggt ccgccagaag ctcgccgaac accgaccgag cacgctcggg
    11041 caggccgcgc ggattcctgg cgtcacgccc gcggccatct cgatcctgct gatccacctg
    11101 aaacggcacc actccgagac gccgggcgcc gcgcgcgtcg ggggcgttgg gtaagatcgc
    11161 gccccaccga gccggtccca ggggacgcga cccatgcacg agccgatcct catgccgcgg
    11221 ccaagccgcg gcggcgcccc gcgtgcccgt cgcgcgtggg tgctcgcgac ggccgcgtgc
    11281 ctggggctcg tggcgtgtgg gggcggggac gatcccacgg gcgggacgtc cggcggcacg
    11341 gccgcgccga acgccggcta cgacccgacg accctgcgtc gcggcaacgg cccggagccg
    11401 gacacgctcg acccgcagct cgctcgcacc gatgccgcgt tcaacatcct gcgcgacctg
    11461 ttcgagggcc tgaccgcagt gggcgccgac ggctcgccgg cgcccgcggc cgccgagtcc
    11521 tggacggtgt cccccgacgg cctcgagtac cgcttcgcgt tacgcgagca gctgcgctgg
    11581 tcgaacggcg acccggtcgt cgcggaagac tacgtggccg gcatgcgccg cctcgtcgat
    11641 cccgccacgg cctcgcccta cgcgcagttc atcgatccgg tcctgaatgc cgccgcgatc
    11701 gctcgcggcg agaaaaagcc ggaggcgctc ggtgtcgcgg cccccgaccc ccggaccgtg
    11761 gtcgtgcgac tcgccgcgcc cgccccgtac ctgctgggcc tgctcgcaca gccgcccacg
    11821 tttccgctgc accgcgcctc gctcgctgcg cacggcgccg aatacgcgcg accgggaaag
    11881 ctgctgtcga acggcgcctt cgtactcgag gactgggtga tcgggtcgca ggtgatcgcg
    11941 cggcgcaaca cgcgctactg gaacgacgcg gagacccggc tcgatcgggt gcattacgtg
    12001 cacgttggcg acgcggggac cgagctccga cagtaccgtg ccggccagct cgacgtgacc
    12061 tacgtcgtgc cgcagcagca gttccagtgg atccggcaga agctcgcggg cgagctgcac
    12121 gtcgccccgc agctgagcgt ctactactac ggcttcaacc tgactcgccc accgttcaag
    12181 gaccagcccg ggctgcggcg cgcgctgtcg atggtgatcg accgcgaccg gctcgtgact
    12241 gcggtgacag gcgtgggcga agcgccggcc tacggctggg tgccgccggg cgtgtggaac
    12301 tacacgccgc agcaattcga ctatgcctcc ctgccgtacg aggcccgggt cgccgaggcc
    12361 cggcgactgt acgcgcaggc gggatacacg gccgagcgtc cactcgaggt ggagctgcgc
    12421 tacagctccg gcgacaccca caaccgcatc gccgtggcgg tcgcagccat gtggcaggaa
    12481 gcgctcggcg tgaagacgca gctgtacgcc gaggagttcc gcgcgctgct gcagagcatc
    12541 cagcgcaagg acacccaggt gtttcgctcg agctgggtgg cggacttcaa cgatgcctac
    12601 acgttcgccc agctgctcaa gtcggacttc ggcatcaacc tgaccggtta ctcgaaccca
    12661 cgttatgacg cgttgctcgc cgaggccgag cgccaggccg acgtcgggcg acgtcgtgag
    12721 ctgctgcagg aggccgagcg cgtgctgctc gccgaccacc ccctgctgcc gctgtacttc
    12781 tacgtcaaca agcacctcgt gaaaccgtgg gtgcgaggct ggacggacaa cgtcatgaac
    12841 gtggcctaca gcaaggatct ttcgctgcag ccagccggtt gagcggtcgg tcacatttcc
    12901 ggcagcactc gacgccgcga agttgagaga cggtgaccgc gcggtccacc atcggcgcac
    12961 tcgttcaccg cgcgcacgca tcgcccatgg cctggttcgt cgtcttcgcc gtcaccgtgt
    13021 tcggcctctg gttctggcgc gaatatcgcg tccagcaacg gcacctcgag agtgcctcgg
    13081 ccccccatcg catgggtgag cgctacatcg gccaggtcat caccatgccg cagggcatcg
    13141 ccaacggcga aggccgcatc aagctcggtc gccaggactg gtccgtgcgg gggccggacc
    13201 tgcccgcggg cggcaaggcg cgtatcaccg gcgtggatgg ccgcgtgctg atcgtggacc
    13261 ggatgccggc cagatagtgg tcagtggcta gtggctagtg gtcagtgggt gctgatcagg
    13321 aaggtttgat aatctgcgcg cgtagcttgt aatcctcggg cagattccca agaaattctc
    13381 acggaacgat cgggctggca accgtctccg gccactagcc actagccact agccactagc
    13441 cactagcctc accgcgcgat ttcgtactgc gcgctgacgt tggcgacgaa cttcatctcc
    13501 cccggggcgt aggtctgctc ggcggcgtcg gcctgcgcag acatcgccgc tcgctccatg
    13561 tacttcggct ggaacggcat cgccgtcgag gcgctcaggc tgtagacggg gcccaggcgc
    13621 gcgctcgcgg ccttggccag ggtctctgcg tcgagccgcg cgtcgtcgac ggccctggtc
    13681 agcgcctcgc gctcgagctc cttgcgacgg gtggagtcga ggcgcgcgcc gccgagcaca
    13741 ttgacgccgg ccgacaccga acgctcgagc agcggcccga ggcgctcgag gtcgcgcaac
    13801 tcgacctcga tctgtcgact cacgacgtag ccgagcagca cgcgctggga gtccttttcg
    13861 ttccagcgat actcgggctg gacttgcagc tggctggagt ccacgaactt cgggtcgatc
    13921 ttgagctcgc gcgtcagcgc gagcacgcgc tcgaccgtgg cggtgacctg cgtgcgggct
    13981 tccgccaggg tcggcttgcg cgcctcgacc ccgagcgtca cgtatgccat gtccggcatg
    14041 gccttgacct cgccctcgcc ggtgacgctg atgagcggtg gcggcaccgt gtcgtgcgcc
    14101 gaggcgacgc cggcgacggc gagtcccgac atcaacgcca cccacaccgc ccgtcccagt
    14161 cccagttgcg cgtgcttcat gcatccgtcc tccggccaat cgtgcgcaca gtctatgtca
    14221 gcgcggtgcg acgaccggca cgaccgggcg tgccggggcg gccattcgac tcgccaaggc
    14281 agattgatta acatgcccgg ccctgtggcc ggacgcggcg gcaggcacga gaacgacgac
    14341 gattccgcag gagttgcaat gatttcgaga tcggcctggg tggcgctgag cctgtcactc
    14401 gcgatgctcg ggcccggcct cgcacacgcg gccgacaagg cccgcggcgg caagctcgcc
    14461 tacacctgcc acggctgtca cggcatcgag aactacaaga acgcgttccc ggtctactcg
    14521 gtgcccaagc tcggcggcca gcacgcggcg taccttgccg ttgcgctcaa ggaataccag
    14581 gacggcaacc gcgcacacgc gacgatgcac gcgcaggcgg cgaccctgaa accccaggac
    14641 gtcgcggacg tgtccgcgta cctggcgggc gtggaactca agtcctcggg acgtccgacc
    14701 ggcacgctgc ccaaggcggg gcagacctgc gtcgcctgcc acggcaacga cggcatcggc
    14761 atcatgcccg agtacccgaa cctggccggg cagcacgagg attacctcgc gatgtcactg
    14821 cggggctaca agaccggcca gcgcaagaac ccgatcatgg ccggcatggc cgcgcccctg
    14881 accgagcagg acatccggga actcgcccgc tattacgcct cgcagcgccc tgcgctttgc
    14941 gcgacggacg acatccgcaa ggccgggcag tgcaagggtc cgtgacgccc accgcatgag
    15001 ctcatgccag aaggccgggt ccgtcccggc cttcttgtct tgggacggca ctggtcgcac
    15061 gctaggcgac cccgtgaatg ctgccgagac catgtccgcc accagtgctt cgccgacgtt
    15121 gcgccatgcc cagccgcagg gcgacgggct gtacctgatc gacaccgatt acgtgcggcc
    15181 ggggctcgcg gccgcacacc tgctcgtcga cgacgggcat gcggccttca tcgacaccgg
    15241 gcccgctccc gcggcgcccc gtctgctcgc cgcgctcgac gaggtcgggc tggcaccgga
    15301 gcaggtcgac tacctgttcc tgacccacgt gcacctcgac cacgccgggg gcgccggcca
    15361 gatgatgcgt gcgctgccga acgcgcgcgc ggtgctgcac ccgcgtggcg ccccgcacct
    15421 cgtcgatccg gcgaagctga tcgcgggctc gatcgccgtc tacggcgaga cgctgtaccg
    15481 ccagctgtac ggcgaactcc tgccgattcc ggccgatcga gtgctgacga ccgaggacgg
    15541 cacgcggctc gagctgggcc ggcgcacgtt cgagttcatc gacgcgcccg ggcacgcccg
    15601 gcaccaccat tgcccaatcg acctcgaccg ccgcgacgtc tacgccgggg acaacttcgg
    15661 catctgttat cgagacctcg acaccgcggc cgggccgttc atgctgccga ccacgacgcc
    15721 cgtgcagttc gacccggagg cgatgcaccg cacgatcgac cggctgatgg cctaccagcc
    15781 gcgccggatc gtacagacgc acttcgggcc ggtcaccgac ctggagcgcc ttgcccgcga
    15841 cctgcacgcg tcggtcgacg aactcgtgcg catcgcgcgc cggtttgcgg atgcgcccga
    15901 tcgcacgcag cgcatccgcg cggcgatgtt cgactacttc agtgcgcgcc tcgacgagca
    15961 cgggtacacg ggcgacccgg ccacgcggca tcggctcatc gacgaggacg tcaacctgaa
    16021 cacccagggc ctcgaagtct ggctgtcgaa gcaatgaaca accctttcac cgcgttccgt
    16081 gtccatcgca tcgacggcaa gatcgtcgcg cgcttcgacc aggtgacgct cgacgacctc
    16141 acgccgggcg acgtcgtcgt ccgcgtgagc tactccgaca tcaactacaa ggacgcgctc
    16201 gccgcgaccg gcgcctcgcc gatcctgcgc cggtatccgc tggtgggagg catcgacctg
    16261 tcgggcgagg tcgtcagttc cgccgaccca cggttcacgc cgggccagaa agtgctggtg
    16321 accggctgcg gcctgagcga gacgcaggac ggcggctacg ccgagtacgc gcggctgcag
    16381 ggcgactggg tgatcccgct gcccgacggc ctcgacgagc gcgacgcgat gcggctcggc
    16441 accgcggggt tcactgcggc gctggcggtc caccgcatgg agcagaatgg ccaggcgccg
    16501 gg
//
LOCUS       DASPFR010000356         2316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_839149, whole genome shotgun sequence.
ACCESSION   DASPFR010000356 DASPFR010000000
VERSION     DASPFR010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839149"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VD737_09440"
     CDS             complement(<1..519)
                     /locus_tag="VD737_09440"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXW10828.1"
                     /translation="MARFFIDRPIFAWVIAIIIMLAGALSITRLPISMYPTIAPPAVT
                     ISASYPGASAEAVENAVTQIIEQNMKGLDGLIYMSATSQASGQAAVSLTFESGTNPDI
                     AQVQVQNKLQLAMPLLPQEVQRQGVSVTKSRTGFLLIIGFVSKDGGMNREDLADYVGA
                     NLRDPLSRVEGVG"
     gene            complement(532..1704)
                     /locus_tag="VD737_09445"
     CDS             complement(532..1704)
                     /locus_tag="VD737_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXW10829.1"
                     /translation="MASLTHAKTAAFPLLLVALAGLAACGQEPEGAEGAGPVPVTVVT
                     LKPETVTLRRELPGRSSAYLVAEVRPQVDGIVLKRLFTEGGLVKQGQPLYQLDDATYR
                     ADLNTARANVVRAQATLETAELNAKRSAQLVKVDAVSQQDHENAVAALREAQAELKAA
                     QATAARAETILGYARITSPIDGRIGKSTVTQGALVTANQSEPLATVQQLDPMYVDLTQ
                     SSAEWLQLRQQLAAGRMEGASDLPVTILLEDGTEYSRPGKLAFSEVSVDPTTGSFGLR
                     VVVPNPDNVLLPGMYVRAVVGTGIRENALLVPQQGVQRDPKGGTTAMVVGDDEKVAVR
                     EVRVSQTVGDKWLVEDGLEAGDRVVVEGLQKIRPGAVVQATEASAEAAPAVASARQ"
     gene            complement(1962..2240)
                     /locus_tag="VD737_09450"
     CDS             complement(1962..2240)
                     /locus_tag="VD737_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HXW10830.1"
                     /translation="MVTALVLIKAATDKIPELAQTMAELDGVTEVFSVAGQYDLVALV
                     RVRENEDLAKVISERMRKLPGITSSETLIAFKVYSRKDIEAAFQLGLD"
BASE COUNT          340 a          770 c          811 g          395 t
ORIGIN      
        1 gcccacgcct tcgacgcggc tcaacgggtc gcgcaggttc gcgccgacgt agtcggcgag
       61 gtcctcgcgg ttcatgccgc cgtccttcga gacgaacccg atgatcagca ggaagccggt
      121 gcgcgacttg gtcacgctga cgccctggcg ctgcacttcc tgtggcagca gcggcattgc
      181 gagctgcagc ttgttctgca cctgcacctg cgcgatgtcc ggattcgtgc cggactcgaa
      241 ggtcagcgaa acggcggcct ggcccgaggc ctggctcgtc gcggacatgt agatcaggcc
      301 gtcgaggccc ttcatgttct gctcgatgat ctgcgtgacg gcgttctcga ccgcctcggc
      361 cgacgcgccg gggtagctcg cgctgatcgt caccgcgggc ggcgcaatgg tcggatacat
      421 cgagatcggc aggcgcgtga tcgacagcgc gcccgcgagc atgatgatga tcgcgatgac
      481 ccacgcgaag atgggtcggt cgatgaagaa acgtgccatg gcgcgcctgc cttactgcct
      541 ggccgaggcg accgcaggcg cagcctcggc gctggcttcg gtggcctgca ccacggcgcc
      601 cggacggatc ttctgcagcc cctcgaccac gacacggtcg cccgcctcga ggccgtcctc
      661 gaccagccac ttgtcgccga ccgtctggct cacgcgcact tcgcgcaccg ccaccttctc
      721 gtcgtcgccg acgaccatgg cggtcgtgcc gcccttaggg tcgcgctgca cgccctgctg
      781 cggcacgagc agcgcgttct cgcggatgcc ggtgccgacc accgcgcgta cgtacatccc
      841 gggcagcagc acgttgtccg ggttcgggac gaccacgcgc agcccgaagc ttcccgtggt
      901 cggatcgacg ctgacttcgc tgaaggcgag cttgccgggc ctggaatact cggtgccgtc
      961 ctcgagcagg atcgtcaccg gcaggtcgga cgcgccttcc atgcgaccgg ccgccagctg
     1021 ctggcgcagc tgcagccact cggcactcga ttgcgtgagg tcgacgtaca tggggtcgag
     1081 ctgctgtacg gtcgccagcg gctccgactg gttggccgtg accagcgccc cctgcgtcac
     1141 ggtcgacttg ccgatgcgtc cgtcgatggg cgaggtgatg cgcgcgtagc cgaggatcgt
     1201 ctcggcgcgc gcggccgtcg cctgcgcggc cttgagttcg gcctgcgcct cacgcagcgc
     1261 ggcgaccgcg ttctcgtggt cctgctggct gacggcatcg accttgacca gctgcgcgct
     1321 gcgcttcgcg ttgagttccg cggtctcaag cgtggcctgc gcgcgcacga cgttggcgcg
     1381 ggcggtgttg aggtcggccc gataggtcgc gtcgtcgagc tggtagagcg gctggccctg
     1441 cttgacgagc ccaccctcgg tgaagagccg cttgagcacg atgccgtcga cctgcgggcg
     1501 gacctcggcg acgaggtatg ccgacgagcg acccggcagt tcgcgccgga gcgtgacggt
     1561 ttcaggcttc agcgtcacga ccgtcacggg gactggtccc gcgccctccg caccttctgg
     1621 ctcctggccg caggccgcca ggccggcgag cgcgacgagc aataggggaa aggcggccgt
     1681 cttcgcgtgg gtgagcgatg ccatggaaac ctcgatcaag cgaaacgggc cggtcgcttg
     1741 agcggccctg agatggagcg agaatgaacg atcattctca ctgggtcaac cgtagcaacc
     1801 gcggcctggc gcgtgcgcta cacgcttcgt cgcacgggcg cggacgggtt cagcggaccg
     1861 gaccaggacg accggcgggg cggcgccggt gtggggtggg gaaaccggtg gacaggtgcc
     1921 gggcgggttt ggacgtcccg cccgaaatca gggggacgac cttagtcgag cccgagctgg
     1981 aacgcggctt cgatgtcctt gcgcgaatag accttgaacg cgatcagcgt ctccgagctc
     2041 gtgatgcccg gcagcttgcg catcctctcg ctgatcacct tcgcgaggtc ctcgttttcg
     2101 cgcacgcgca ccagcgcgac gaggtcgtac tgcccggcga ccgagaacac ctccgtgacg
     2161 ccgtcgagct ccgccatggt ttgcgcgagc tccggaatct tgtcggtggc ggccttgatc
     2221 aggacgaggg cggtgaccat gggagggagg aagtggctag tggccagtgg ttagtggtca
     2281 gtgaagaact cgcgttctcc gttcgcgcag attacg
//
LOCUS       DASPFR010000357         4601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_107912, whole genome shotgun sequence.
ACCESSION   DASPFR010000357 DASPFR010000000
VERSION     DASPFR010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107912"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VD737_09455"
     CDS             complement(<1..998)
                     /locus_tag="VD737_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXW10831.1"
                     /translation="MRIVPLLSLTLSLLGLPAFGAGAELASAGVSPSDEPVASGTQSL
                     IVKFREAPAGIGAAKSAARDVSVTAERARAAAVRAGLVLVESREIAAALQTVKVRATS
                     SRDTLATTLARLRTDPAVEYAEPDHRRYVQAAPDDPIFQGSQWYLKNESSTPSAIDAV
                     GAWDTTQGADDVVVAVIDTGVRFEHPDLRRASAGGRLLDGYDFITDITTANDGDGRDA
                     DASDAGDWCQESGSSWHGTRVSGIVGALTNNATGIAGATWRGRILPLRGLGKCGGNDS
                     DLIAATLWAAGIPVAGVPDNPNPAKVINLSFGSVGACPASWRDAIARVSARGTLVVV"
     gene            complement(1084..1920)
                     /locus_tag="VD737_09460"
     CDS             complement(1084..1920)
                     /locus_tag="VD737_09460"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HXW10832.1"
                     /translation="MIVHLIDGTYELFRHFYGQRRFNKGNDKPFGAVAGVLHTVLETI
                     EGGATHLGVATDHVIESFRNDLWPGYKTGAGIDPALWAQFHPLERALASMGVATWPMV
                     ELEADDALASAAHLAVSDQRVQKVCIWTPDKDLAQCVQGDRVVQVDRKSGKILDATGV
                     REKFGVDPEHIPDFLALVGDAADGYPGLAGIGKVGAVRLIRQFGRLEDFPKDVLGGER
                     RESALLFKTLATLRTDAPLFGRIDELEWRGPGADFPEHARIIGDGRLLERARKACSRL
                     DE"
     gene            2152..3090
                     /locus_tag="VD737_09465"
     CDS             2152..3090
                     /locus_tag="VD737_09465"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF007380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXW10833.1"
                     /translation="MNAAMLRDTLQQLQRATHDHREWHEQFLRTLVCGLPGREDDLAS
                     DAHQRCRFGHWYYVDAPAELRELPAFAEMEAQHALLHRIAASMTRRQAEGQSIPADEY
                     QQYVTAEEALRRELGSLRHEIQDILRSTDPLTGAFGRARLLPELRAWRELAARDVEPC
                     CIAFMDVDHLKTINDVHGHTVGDRVLQGLVSYLAQHLRRYDKVFRYGGDEFLLPLPAT
                     DLQQAEHLVERIRAGIAEVPFVVSADGRPIHATASFGIAALDPDISVDESIDRADRAL
                     LQAKTSGRNRVVSWDPGIATGTILAWRREGTPEPGA"
     gene            complement(3100..3345)
                     /locus_tag="VD737_09470"
     CDS             complement(3100..3345)
                     /locus_tag="VD737_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003108924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXW10834.1"
                     /translation="MPIAVKLDDLLYERRMTLTELADRVGMTLANLSILKTNKARAIR
                     FSTLEAICEVLECQPGDLLRWEPESRARKRPARSASG"
     gene            complement(3347..3817)
                     /locus_tag="VD737_09475"
     CDS             complement(3347..3817)
                     /locus_tag="VD737_09475"
                     /inference="COORDINATES: protein motif:HMM:NF022636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HXW10835.1"
                     /translation="MLVVLNVLYAVAIAVLLVAMPTEQWIMTSLKLSPSPEADRVIAG
                     LHAVAVIGLVAAPLNHAILKRLLSMVETVRAGDPFTVTNAQRLQVIAWVLLALQLLGL
                     VVGGIAEVISTPEHPIDLDAGPAVGGWLAVLLTFLLARVFAHGAAMRDDLEGTV"
     gene            complement(3916..4344)
                     /locus_tag="VD737_09480"
     CDS             complement(3916..4344)
                     /locus_tag="VD737_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10836.1"
                     /translation="MDQIATADLLELLLMQRDSIDLQFQFWLTITFAAIAAGHVAGPR
                     LQYGLRALITGLYVLASAHIALRWSYDGAVGSRWAAELVRRGVDIGVPWAAVYLRTGL
                     MLLGTVSAAVFLMRRPRGLARSVPASGVISKPDDRLDDNQ"
BASE COUNT          723 a         1616 c         1566 g          696 t
ORIGIN      
        1 accaccacga gcgtgccgcg cgctgagacc cgcgcgattg cgtcgcgcca tgaggcgggg
       61 cacgcgccga cgctgccgaa gctcaggttg atcaccttgg cggggttcgg gttgtccggc
      121 acgcctgcca ccgggatgcc ggccgcccac agcgtggcgg cgatcaggtc ggagtcgttg
      181 ccgccacact tgccgaggcc gcgtaacggc aggatccgcc cccgccaggt ggcgcccgcg
      241 atccccgtcg cgttgttggt cagcgcgccg acgatgccgg acacgcgcgt gccgtgccac
      301 gagctgccgg actcctggca ccagtcaccg gcgtcggagg catcggcgtc acgcccgtcg
      361 ccgtcgttgg ccgtggtgat gtccgtgatg aagtcgtagc cgtcgagcag ccgcccgcct
      421 gcggaggcac gtcgcaggtc gggatgctcg aaacgcacgc ccgtgtctat cacggccacg
      481 acgacgtcgt ccgcgccctg cgtcgtgtcc caggcgccga ccgcgtcgat ggcgctcggc
      541 gtgctgctct cgttcttgag gtaccactgc gagccttgga agatcgggtc gtcgggcgca
      601 gcctggacgt agcgccggtg atcgggctcg gcgtactcca cggccggatc ggttcgcagc
      661 cgcgcgagcg tcgtcgccag cgtgtcgcgg gaactcgtcg cccgcacttt caccgtctgc
      721 agggcggccg cgatctcgcg cgactcgacg agcaccaggc cggcgcgcac ggcggccgcg
      781 cgcgcgcgct ccgcggtgac cgacacgtcc ctcgcagcgc tctttgcggc gccgatgccg
      841 gcgggcgcct cgcgaaactt cacgatcagt gactgcgtgc ccgacgcgac cggctcatcg
      901 gacggcgaca cgccggcgga cgcgagctcc gcaccggcgc cgaaggcagg caggccgagc
      961 agcgacaggg tcaacgacag cagtgggacg atgcgcatgc gtgaattgag tgacagggga
     1021 agcctcgcgg gttcaagcgt gcctttggcc ggcgaggata gcctcgccgc gggacgaaat
     1081 cccctactcg tccaggcggc tgcacgcctt gcgtgcccgc tcgagcagcc ggccgtcgcc
     1141 gatgatccgg gcatgctcgg ggaaatccgc gcccgggccg cgccactcga gctcgtcgat
     1201 gcgcccgaac agcggcgcgt cggtcctcaa ggtggccagc gtcttgaaca gcagcgccga
     1261 ctcgcgacgc tcaccgccga gcacgtcctt cgggaagtct tcgagcctgc cgaattgccg
     1321 tatgagtcgt acggcgccga ccttgcctat gcccgcgagc ccgggataac cgtccgctgc
     1381 gtcaccgacc aaagcgagaa aatcagggat gtgctccggg tccacgccga acttctcgcg
     1441 cacgccggtg gcatccagaa ttttcccgga cttgcggtcg acctgcacca cgcggtcgcc
     1501 ctgcacgcac tgcgccaggt ccttgtcggg cgtccagatg cacaccttct gcacgcgttg
     1561 gtccgaaacg gcgaggtgcg cggccgaggc gagcgcatcg tccgcctcga gttcgaccat
     1621 cggccaggtc gcgacgccca tcgaggcgag cgcccgctcg agcgggtgga actgcgccca
     1681 cagcgccggg tcgatccccg cgcccgtctt gtagcccggc cacaggtcgt tccggaacga
     1741 ctcgatgacg tggtccgtcg cgacgccgag gtgcgtggcg ccgccctcga tggtctcgag
     1801 caccgtgtgc aggacgccgg cgacggcgcc gaacggcttg tcgttaccct tgttgaagcg
     1861 acgctgtccg tagaagtggc gaaacagctc gtacgtcccg tcgatcaggt ggacgatcac
     1921 tcggtatatc ccgtcccgcg cgtggagtcg tgcacgcaca agatagactg aagccagcct
     1981 gccggcacct ccgccaacgg gtggtgactc gccgccgact tcgcctggtc gcccgcccgg
     2041 tttcgtggcc cagcgcccat tcgtggcggt tgcggatgcc cgccacgccc ggactgtggc
     2101 agtgtaatca cgtagacgtc gacatcgagc cggaggccgc gtttgttcag gatgaacgcc
     2161 gccatgctgc gcgacacgct gcagcaattg cagcgggcca cccacgacca ccgtgagtgg
     2221 cacgagcagt tcctgcgtac gctcgtctgc ggcttgccgg gccgcgagga cgaccttgcc
     2281 agcgacgccc accagcgctg ccgcttcggc cactggtatt acgtcgatgc tccggccgag
     2341 ctgcgcgagc tgccggcgtt cgcggaaatg gaggcgcagc acgcgctcct gcaccgcatc
     2401 gcggcgtcca tgacacgccg gcaggccgaa ggccagtcca tcccggccga cgaataccag
     2461 cagtacgtga cggccgagga agccttgcgg cgggaactgg gttcgttgcg ccacgaaatc
     2521 caggacatcc tgcgcagcac cgaccccctc accggcgcgt tcgggcgcgc gcgcctgctg
     2581 ccggagctgc gcgcgtggcg agagctcgcg gcgcgtgacg tcgagccgtg ctgcatcgcg
     2641 ttcatggacg tcgaccacct gaagacgatc aacgacgtgc acgggcacac ggtgggcgat
     2701 cgcgtgctgc aggggctggt ctcgtacctg gcgcagcacc tgcggcggta cgacaaggtg
     2761 ttccgctacg gtggcgacga gttcctgttg ccgttgccgg cgaccgacct gcagcaggcc
     2821 gagcacctgg tggagcgcat ccgtgccggg atcgccgagg tcccgttcgt cgtctcggcc
     2881 gacggccggc cgatccacgc caccgcatcg ttcggcatcg cggcgctgga tcccgacatc
     2941 agcgtcgatg aatcgatcga tcgcgccgac cgcgcgctgc tgcaggcgaa gacgtccggc
     3001 cgcaaccgcg tcgtcagctg ggacccgggc atcgccaccg ggacgatcct ggcgtggcga
     3061 cgcgagggga cgcccgaacc tggcgcgtga ggggcaggtc tagccgctcg cgctgcgcgc
     3121 ggggcgcttg cgcgccctgc tctcgggctc ccaacgcagc aggtcccctg gctggcactc
     3181 gagcacctcg cagatcgctt cgagtgtcga gaagcgaatc gcgcgtgcct tgttggtctt
     3241 gaggatggaa aggttggcga gcgtcatgcc gacccggtcc gcgagttcgg tcagggtcat
     3301 gcggcgctcg tacaacaggt cgtcgagctt gacggcgatc ggcacgtcag accgtccctt
     3361 cgaggtcgtc gcgcatcgcc gcgccgtgcg cgaacacgcg tgccagcagg aacgtaagca
     3421 gcacggccag ccagccgccc accgccgggc cggcatcgag gtcgatcggg tgctcgggcg
     3481 tcgagatgac ttcggcgata ccgcccacga ccagcccgag gagctgcagc gccagcagca
     3541 cccaggcgat gacctgcagc cgctgcgcat tcgtcacggt gaacgggtcg cccgcgcgca
     3601 ccgtctcgac catcgaaagc agtctcttca ggatggcgtg gttcagcggg gccgccacca
     3661 ggccgatgac cgcgaccgca tgcaggccgg cgatcacccg gtcggcctcg ggcgaaggcg
     3721 acagcttcaa cgaggtcatg atccattgct cggtgggcat ggcgacgagc aggacggcga
     3781 tggccaccgc gtaaagcacg ttgaggacga ccagcactcg gagcacggcc cgcgcgagcg
     3841 gcagcgcggc tgacgacaag gtgttgttgg accgcatggc ctcgacctcg gttttatcga
     3901 taatcaatac tcctgttatt gattatcgtc aagccggtcg tcgggtttcg atatgacgcc
     3961 acttgccgga acggacctcg cgagtccgcg tgggcggcgc atcaggaaca cggccgcgct
     4021 cacggtgccc agcagcatca gtcccgtgcg caggtacacc gcggcccacg ggacgccgat
     4081 gtccacgcct cgccggacaa gctcggcagc ccagcgcgac ccgacggcgc cgtcataact
     4141 ccagcgcagc gcgatgtgcg cgctcgcgag cacgtagagc ccggtgatca gcgcacgcag
     4201 gccgtactgc agccgtgggc cggccacgtg cccggcggcg atcgcggcga acgtgatcgt
     4261 cagccagaac tggaactgca ggtcgatcga gtcccgctgc atcagcagca gctcgagcag
     4321 gtcggccgtc gcgatctggt ccatcgttgg cgctacgata cacgaggcca ctggaattcc
     4381 agtgctttgg cgcgatcgaa gccgatcctc gccgcacgcg ggacggcgcc cgcggacccc
     4441 tcaccccggc ccgatcgtcg cgagcatcca cgtcgccatc accagcagcg tgcactgggc
     4501 cacgccccgc acgaccgcgg ccatgatgtg aaccttcatg tgatcctcct ttggacgacg
     4561 cgcctggggt gcaggcgcgc gtcccgattg cgattgagtc g
//
LOCUS       DASPFR010000358         2933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1448999, whole genome shotgun sequence.
ACCESSION   DASPFR010000358 DASPFR010000000
VERSION     DASPFR010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1448999"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VD737_09485"
     CDS             <1..605
                     /locus_tag="VD737_09485"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXW10837.1"
                     /translation="PPHVTAAVGMDALSHNLEAYCAPGYHPMAEGIAVEGMRLIKEYL
                     PRAVANGADLEARSHMLVASSMGATAFQKGLGAMHSLSHPCSANLNTHHGLTNAVVMP
                     YVLEWNREVLEDKTARLAAWLGLAEHSYAGVLKWVLQLRRTIGIPETLAGLGLEEQHA
                     RSFAPQAYADPSTGGNPVPMTERCFEQLYLRCIRGELSPS"
     gene            651..1157
                     /locus_tag="VD737_09490"
     CDS             651..1157
                     /locus_tag="VD737_09490"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HXW10838.1"
                     /translation="MQISMYRASVPVLARALRNLAAVLRKGEAHAAARGIDPSVLLGY
                     RLAPDMFPLTRQVQIASDTAKGCVARLAGVEVPSYPDDEATFAQLLARIERTIAFIEG
                     LPPAQIDGSEEKPVTLKMRTGEINFQGLPYLLEFVLPNVYFHSTTAYAILRHCGVELG
                     KKDFLGVT"
     gene            complement(1238..1705)
                     /gene="hybE"
                     /locus_tag="VD737_09495"
     CDS             complement(1238..1705)
                     /gene="hybE"
                     /locus_tag="VD737_09495"
                     /inference="COORDINATES: protein motif:HMM:NF023365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[NiFe]-hydrogenase assembly chaperone HybE"
                     /protein_id="HXW10839.1"
                     /translation="MSEAAPSRVERLVAYYERAARNMQGLPMYNAALAVEAVGFREHD
                     GRLVGVIVTPWFMNLTLLPSPADLQAWRKGSNARVPFPSGQYDFAVGDAGENDLIATC
                     SLFSLMHDFIDHEAARATALAAAEALFEPPAPPPAAQRAASPAMSRRRFLRGG"
     gene            complement(1702..2100)
                     /locus_tag="VD737_09500"
     CDS             complement(1702..2100)
                     /locus_tag="VD737_09500"
                     /inference="COORDINATES: protein motif:HMM:NF019092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10840.1"
                     /translation="MDAPRAAPDSLIEQLARDPASEVVRRSGLQDFLARHPRALLFFT
                     GDLARNPEGRDVAIVVRELLRQYAGRVALGLVDRRDETAVMAKTGVVVLPAVAFVADG
                     RTVDVVARMRDWGAYAQAAERLFADGDPST"
     gene            2284..>2933
                     /locus_tag="VD737_09505"
     CDS             2284..>2933
                     /locus_tag="VD737_09505"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HXW10841.1"
                     /translation="MILSSVARCTTLAFALVVTACAPFRPVPETITRGDSRWVATRWD
                     ELPGWRDDAVHEAWPALLRSCERPSPEWASACASARAATAPRNDAQARAWFVDHLQPY
                     RLEPLRGAPPGLITGYYEPTFDARRVPGGEFRVPLHALPPDLATRKPYWTRQQIETLP
                     AAQSALRGRELVYLADPLDALALQIQGSGRTRVTEADGRLKVVRMAYAGHNDQPYRS"
BASE COUNT          445 a         1052 c         1010 g          426 t
ORIGIN      
        1 tgccgccgca cgttaccgcc gcggtcggga tggacgcgct gtcgcacaac ctcgaagcct
       61 attgcgcccc cgggtatcac ccgatggccg aaggcatcgc cgtcgagggc atgcggctga
      121 tcaaggaata cctgccgcgg gcggtcgcga acggtgcgga cctcgaggcg cgctcgcaca
      181 tgctcgtggc ctcgtcgatg ggcgccacgg ccttccagaa aggtctcggc gcgatgcaca
      241 gcctgagcca cccgtgctcg gccaacctga acacccatca cggcctcacc aacgccgtcg
      301 tgatgccgta cgtgctcgag tggaaccggg aggtgctcga ggacaagacc gcgcgtctcg
      361 cggcctggct gggcctcgcc gaacactcgt acgccggcgt gctgaagtgg gtgctccaac
      421 tgcgacgcac gatcggcatc ccggagacgc tggccgggct cggcctcgag gaacagcatg
      481 cgcggtcgtt cgcaccgcag gcgtatgcag atccgtcgac gggcggcaac ccggtgccca
      541 tgaccgagcg ctgcttcgag caactctacc tgcgctgcat ccgcggcgaa ctctcgccga
      601 gctgaaccgg gcggcgtcga ttagactgcg cgctttcgct ggaggatcgc atgcagatct
      661 cgatgtaccg tgcctcggtg cccgtgctcg cccgggcctt gcgcaacctg gccgccgtcc
      721 tgcgcaaggg cgaggcgcac gccgccgccc gcggcatcga tccgtccgtg ctgctcggat
      781 acaggctggc gcccgacatg tttccgctga cccgacaggt gcagatcgcc tcggacaccg
      841 ccaaggggtg cgtcgcgcga ctcgccggcg tcgaggtgcc gtcttatccg gacgacgagg
      901 cgacgttcgc gcagctgctc gcgcgcatcg agcgcacgat cgcgttcatc gaaggcctcc
      961 cgcccgcgca gatcgacggc tcggaagaga agccggtcac gctcaagatg cgcacgggcg
     1021 agatcaactt ccaggggctg ccgtacctgc tcgagttcgt gctgccgaac gtgtacttcc
     1081 acagcacgac cgcgtacgcg atcctgcggc actgcggcgt ggaactcggc aagaaggatt
     1141 tcctgggcgt gacctgaacg gccgaggccg ggtggaacac ccggtcgctc gtgggcgtcc
     1201 gacgggatcg cgacgcggtc ccgatggcgt gccacgctca acccccgcgc aggaacctgc
     1261 gccgcgacat cgcgggtgac gccgcacgct gcgctgccgg tggtggtgcg ggcggctcga
     1321 acagcgcctc ggcggcggcg agcgccgttg cgcgcgcggc ctcgtggtcg atgaaatcgt
     1381 gcatcaacga aaacagcgag cacgtggcga taaggtcgtt ctcgccggcg tcgccgactg
     1441 cgaagtcgta ttggccggac ggaaacggca cgcgcgcatt cgagcccttg cgccaggcct
     1501 gaagatcggc cggcgagggc agcaacgtca ggttcatgaa ccacggcgtc acgatcacgc
     1561 cgacgaggcg gccatcgtgc tcgcggaaac cgaccgcctc gaccgcgagg gcggcgttgt
     1621 acatcggcag gccctgcatg ttgcgggccg ccctctcata ataggcgacc agcctttcga
     1681 cgcgcgacgg cgctgcctcg ctcacgtcga cgggtctccg tcggcgaaca accgttcggc
     1741 cgcctgcgca taggcgcccc agtcgcgcat gcgcgcgacg acgtcgacgg tgcggccgtc
     1801 cgcgacgaat gcgaccgcgg gcaggacgac gacgccggtc ttcgccatca ccgccgtctc
     1861 gtcgcgacga tcgacgagcc cgagcgcgac gcggccggcg tactggcgca gcaactcccg
     1921 caccacgatc gccacgtcgc ggccttcggg gttccgggcg agatcgccgg tgaagaacag
     1981 cagggcccgc gggtgccgcg cgagaaaatc ctgcaggccg gaccgtcgca ccacttcgct
     2041 ggccgggtcc ctggccagtt gttcgatcag cgaatcgggc gcggcgcggg gtgcatccat
     2101 ggacgcgtgc aggatgaccg ggcctgcgcg acgcggtcaa gcgtgccggt gggcgtggtc
     2161 gatgatgagc gtgcgcgcgg ccgtgccggt cacggcgcgg gtccgcggcc gctagaatgc
     2221 gcagcctcgc aactccgtca ctgcctcgtg ccgccacctc acgcccgcca ccgtgcaact
     2281 gccgtgatcc tctcgagcgt tgcgcgctgc acgacgctgg cgttcgcgct cgtggtcacc
     2341 gcctgcgcgc cttttcgtcc ggtgcccgag acgatcacgc gcggcgactc gcgctgggtc
     2401 gcaacccggt gggacgagct gcccggctgg cgcgacgacg cggttcacga agcatggccg
     2461 gccctgctgc gaagttgcga gcggccgtca cccgaatggg ctagcgcgtg tgcctcggcg
     2521 cgcgccgcca cggcaccgcg caacgacgcg caggcccggg cctggttcgt cgatcacctg
     2581 cagccctatc gcctggagcc gctgcgcggc gcgcccccgg gcctgatcac cgggtactac
     2641 gagcccacgt tcgacgcgcg tcgcgtgccg ggcggcgagt tccgcgtgcc gctgcacgcc
     2701 ttacctcccg acctcgcgac ccgcaagccg tactggacac ggcagcagat cgaaacgctg
     2761 ccggcggcgc agtctgccct gcgcggccgc gaactcgtgt acctggccga cccgctcgac
     2821 gcgctggcgc tgcagatcca gggatccggg cgcactcgcg tcaccgaggc cgacgggcgc
     2881 ctgaaggtcg tgcggatggc ctatgccggt cacaacgacc agccgtatcg ctc
//
LOCUS       DASPFR010000359         3227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_473261, whole genome shotgun sequence.
ACCESSION   DASPFR010000359 DASPFR010000000
VERSION     DASPFR010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473261"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..551
                     /locus_tag="VD737_09510"
     CDS             141..551
                     /locus_tag="VD737_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10842.1"
                     /translation="MDGPATPASGDRTRYHAGAPVTFDDLEVLACVYGPRTSPADVER
                     MMPLGPGWARGIAQWRAAGSTYLLLVARTFDGGSRDEPQAVLANDHATLERAIWLAIG
                     DCDQRLCLLESFEPSVVPFVRTVIAASRAAPGHA"
     gene            complement(542..1441)
                     /locus_tag="VD737_09515"
     CDS             complement(542..1441)
                     /locus_tag="VD737_09515"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXW10843.1"
                     /translation="MARVTEPYRPRRPPEFLQRELCGVLHHLTVWPGVDPPVVLLHGF
                     MDCGATFQFVVDELVTGRTVLAPDWRGFGRSAWAIGGYWFPQYFADLEALLDACAIHS
                     ADLVGHSMGGNVALAYAGVRPERVRSVVTLEGFGLAGSPPETAPIRYRDWLDQLQRPD
                     PSTVFPSHAVLVDVLRKRNPRLTPARAAFVAHAWAESLDDGRARLRFDPAHKRVNPVL
                     YRRAEAEACWRRIAAPVAYVAGSESEFLARLDGAGDPDRMRALIPHLEPHVVEGAGHM
                     LHHDQPARVAAIIDGFFERGRQA"
     gene            complement(1475..>3227)
                     /locus_tag="VD737_09520"
     CDS             complement(1475..>3227)
                     /locus_tag="VD737_09520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXW10844.1"
                     /translation="TRLKQIVERGAGTFTTRYRRKDGSVLPLEVSARTLEIDGRLYVQ
                     AIGRDVSERLEQERRLAALAADRDRALARLELQFERMASACIVLGTDGTMLQVNPAFE
                     RMFEIEAAQVVDRPVLDFVRLPRFREEVITRLEALRADPESSYTGVHENITARGRAII
                     CRWNGAALRARDGAVHGYVVMAEDITEVVRSEQALRESEARYRALSDVSPVGIFRTDF
                     AGELLFANPRASAITGIPLDASLGHGWRRAVHPADGAAVKAAWDDYVASAGRLVYSLE
                     FRIVRPDGAEAWVLSQVMPERGDDGQVIGHIGTITDITAVKQAQFELQQAHDLLEERV
                     RERTQQLEAAKDAAEHSDRVKSAFLSTMSHELRTPLNSILGFTDVLLNRLAGPLTDEQ
                     ARQLRLVREASTTLRTLVEDVLDVSRIEAGQVGLEYDDVDLAELVARRVMLFGDVAER
                     KGIALRVETSAGGPVIRSDGRRVGQIVGHLVSNAIKFTDAGEVVIDVRAAGSGVEVAV
                     TDTGIGIPEAWLAQLFNPFVQVARPGGRLRDGTGLGLAISRNLARALGGDITVHSEPG
                     RGSRFTLWLPGVAAAAA"
BASE COUNT          483 a         1165 c         1117 g          462 t
ORIGIN      
        1 gtgattagcg gtgagtcgga gctgaacgcg gatgtgctct ctcccacact cttcctaacc
       61 gctacccgcc aaccgccaac cgcttcctaa ttggcgacga ttgcccgccc gcgaactgcg
      121 ccctcgctag tctgggccgc atggacggac ccgcgactcc cgcctcgggc gaccgcacgc
      181 gctatcacgc gggcgctccc gtcacgttcg acgacctgga agtgctcgcg tgcgtgtacg
      241 gtccgcgcac gagcccggcc gacgtcgaac gcatgatgcc gctcggcccc ggctgggcac
      301 ggggcatcgc gcagtggcga gcggccggct caacgtacct gctgctggtc gcgaggacgt
      361 tcgacggcgg ttcccgcgac gagccacagg ccgtgctggc gaacgaccac gcgaccctcg
      421 agcgcgccat ctggctcgcg atcggtgact gtgatcaacg gctctgcctg ctcgagtcgt
      481 tcgagccgtc ggtggtgccg ttcgtccgca ccgtgatcgc ggcgtcccgc gccgcgcccg
      541 gtcacgcctg acgtccccgc tcgaagaagc cgtcgatgat cgcggccacg cgggccggct
      601 ggtcgtggtg cagcatgtgc ccggccccct cgacgacgtg cggctcgagg tgcgggatca
      661 gcgcacgcat gcggtcggga tcgcccgcgc cgtccagtcg tgcgaggaac tcggactcgg
      721 agccggccac gtaggccact ggcgcggcga tgcggcgcca gcacgcctcg gcttccgcgc
      781 gacggtagag gacggggttg acgcgcttgt gcgccggatc gaatcgcaac cgtgcccgac
      841 cgtcgtccag agactcggcc caggcgtgcg cgacgaacgc ggcccgcgcg ggcgtgagcc
      901 tggggttgcg cttgcgcagc acgtccacca gcaccgcatg cgacggaaac accgtggacg
      961 gatctggccg ctgcagctgg tcgagccaat cacgatagcg gatgggcgcg gtctccggtg
     1021 gcgagcctgc caggccgaac ccctcgagcg tcaccacgct gcgcacacgc tcggggcgca
     1081 cccccgcgta ggcgagcgcg acgttgccac ccatgctgtg gccgaccagg tccgcggagt
     1141 ggattgcgca cgcatcgagc agggcctcga ggtcggcgaa gtactgcggg aaccagtagc
     1201 ccccgatggc ccaggccgag cggccgaatc cgcgccagtc cggcgccagc acggtgcgac
     1261 cggtgacgag ttcgtcgacg acgaactgga acgtcgcccc gcagtccatg aatccgtgca
     1321 acaggacgac aggcgggtcg acgccgggcc agaccgtcag gtgatgcagc acgccgcaga
     1381 gctcgcgctg caggaactcg ggcggacgcc gcggtcgata gggttccgtc acgcgcgcca
     1441 tggtgccgca cgcgcacgcg gctgtcgcgg cgggtcaggc cgcagcggcc gccacgccgg
     1501 gcaaccagag cgtgaagcga ctgccgcgac cgggctcgct gtgcacggtg atgtccccgc
     1561 cgagggcacg tgccaggttg cgcgagatcg cgaggccgag ccctgtgcca tcccgcaggc
     1621 gcccgccggg acgcgcgacc tgaacgaacg gattgaagag ctgggcgagc cacgcttccg
     1681 ggatcccgat gcccgtgtcg gtgaccgcga cttcgacccc gctccctgcc gcgcgcacgt
     1741 cgatcacgac ctcgccagca tccgtgaact tgattgcatt ggagacgagg tggccgacga
     1801 tctggccgac gcgccggccg tcgctgcgga tcaccgggcc gccggccgag gtctcgacac
     1861 gaagcgcgat gcccttgcgc tcggcgacgt cgccgaacag catcacccgg cgcgcgacga
     1921 gttcggccag gtcgacgtcg tcgtactcga gcccgacctg gcccgcctcg atccgggaaa
     1981 cgtcgaggac gtcctcgacg agcgtgcgca gcgtggtcga cgcctcgcgg acgaggcgca
     2041 gctggcgcgc ctgctcgtcg gtgagcggac cggccagccg gttcagcagc acgtcggtga
     2101 aaccgaggat ggaattgagc ggcgtgcgca gttcgtggga catcgtcgac aggaacgccg
     2161 acttgacgcg gtcggagtgc tcggcggcgt ccttggccgc ctcgagctgc tgcgtgcgct
     2221 cgcgcacacg ctcctcgagc aggtcgtgcg cctgctgcag ctcgaactgc gcctgcttga
     2281 cggccgtgat gtccgtgatc gtgccgatgt gcccgatgac ctggccgtcg tcgccgcgct
     2341 cgggcatcac ctgcgacagc acccacgcct cggcgccgtc cgggcgcacg atccggaatt
     2401 cgagcgagta caccagtcgt ccggcgctgg cgacgtagtc gtcccaggcc gccttgaccg
     2461 cggcaccgtc cgccgggtgg accgcgcgcc tccaaccgtg gcccagcgac gcgtcgagcg
     2521 ggatcccggt gatcgcgctg gccctgggat tggcgaacag cagctcgccg gcgaagtccg
     2581 tgcggaagat gccgaccggc gagacgtcgc tcaacgcacg gtagcgtgcc tcgctttccc
     2641 ggagcgcctg ctcggaccgc acgacctcgg tgatgtcctc ggccatgacc acgtagccgt
     2701 gcaccgcccc gtcacgcgcg cgcaacgccg cgccattcca gcggcagatg atcgcgcgac
     2761 cgcgcgcggt gatgttctcg tgcacgccgg tgtagctcga ttccggatcc gcgcgcagtg
     2821 cctcgagacg cgtgatgact tcctcgcgga accgcggcaa ccgcacgaag tcgagcaccg
     2881 ggcgatcgac gacctgcgcg gcctcgatct cgaacatgcg ctcgaacgca gggttcacct
     2941 gcagcatcgt gccatcggtg ccgagcacga tgcacgccga cgccatgcgt tcgaactgca
     3001 gctcgagtcg tgcgagggcc cggtcgcggt ccgcagccag ggccgcgagg cggcgctcct
     3061 gctcgagccg ctcgctgacg tcgcggccga tcgcctgcac gtagaggcga ccgtcgatct
     3121 cgagcgttcg cgcgctcact tccagcggca gcaccgagcc gtccttgcgc cggtaacggg
     3181 tcgtgaacgt tcccgcaccg cgctcgacga tctgcttcaa ccgcgtc
//
LOCUS       DASPFR010000360         8968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_595534, whole genome shotgun sequence.
ACCESSION   DASPFR010000360 DASPFR010000000
VERSION     DASPFR010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8968
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595534"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2522)
                     /locus_tag="VD737_09525"
     CDS             complement(<1..2522)
                     /locus_tag="VD737_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXW10845.1"
                     /translation="MSVYRPRSTSLARIVSAVLAAAAPAALHAQAPEEPTTAAGGLEE
                     VLVTATRAGVTNLQQTPVSVSAVTTEDIDRMIARDISGIASSVPGFSAARVTAFNAAS
                     FAMRGVGVTDIIVYQDAPVSVQVDDFVMPSVQTQLLDTFDTYSVEVLRGPQGTLFGKN
                     TTGGAVTIQTKRPDMDEFGADFRLAYGSFNSERVQGSIDLPLVDDVFAMRFVGAYSKS
                     DGYYKLGADYGPINTLNIFGGTFAPFTIHGVTGQSGGGQPGKSAGGDDIVNGRIKAQW
                     QPTEDLTFLAQYEIMRDRSDAVPAYNDTPPDGPYLWNVLGFTRPSGDPLDNMGATQRN
                     DSLLKMGKGQVIDVDGVYLNMEWDLGHGTIYAVAGKRDQDEHLPNTYTGAAPVNVLTG
                     EVISLFDATRDTTRETTQFEARYASKFDGPVNFVAGAFQQTNDASFCVVQVLGFIDLA
                     FDLGSIGAPQQLNNSTPQVLCNRQDSDSLAGYVDVTWDVTDQFSLTGGYRMTRDEREW
                     AGRTQVGFDILDDNTLNGSLSWQDFNDPLQAGNFNKYPGGIIVNKNTPGFENLSEEWT
                     EPSWRVIGSYKFTDNLFSYLTVSRGYKAGGYNDQTGTSGLMVSELTRPVDPEFATNYE
                     LGMKWESEDSRFRFNPTVFFTKYEDAQRAVNIITVNSAGATFQETVFYNAAEVESKGV
                     ELEFQALLTDAFRLRAQASFLDAEYKEFVINQPEVVDPVTGATIQAFNEDFSGVPVPR
                     SPEFMGSIQALYTWTLGSGAALEFGAEYYHEDENLFYISAAGSEYDAYLDEKNLLNAS
                     VTYTGADGNWFVRGYGRNLTDERYRIASQSVATLWTHTQWGAP"
     gene            2864..4225
                     /locus_tag="VD737_09530"
     CDS             2864..4225
                     /locus_tag="VD737_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HXW10846.1"
                     /translation="MAANLKPAWPMSLSAPVDSLEAKQPYVDVGHDVVDPSRYYSREF
                     MELEWKFLWPRVWLLAGVTSDVPEEGDYTVFNHGHESIVIVRQEDGGLRAFFNACPHR
                     GNPIALNERGSVAKFTCAFHAWRFCLDGKLDCITDEHSFDRGLVAHRPGLTEVRCDQV
                     GGLIFVNMDGKAPPLREWLGLPPGYIENYQIDRMHVVRHVRTEWFANWKTGVDAFYET
                     YHLPHIHPQTQCVMEDYSQYDQYPNGFTRMIVPICTPSHRVIDRDSVNDGLKYMMLEA
                     GMDPAAFQGTGIEVRTAVQKAKRERAKRLGLDHYDKFTDGQLTDSWATGYFPNVQIGM
                     HPEGVFIMRFQPHPTDPERFFYDNMTLIRYVDDPNYSVPGWMGLPKGTDLTGATRPNI
                     EHHAAGVRADLGEVLNQDVDLVEAVQRGQKSRGFRGPLWSEQEIRLRHYHHEIDRYLR
                     GEK"
     gene            4222..4674
                     /locus_tag="VD737_09535"
     CDS             4222..4674
                     /locus_tag="VD737_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXW10847.1"
                     /translation="MTVRRCYDDLVVGEVRESGEHVVELDALVAFAREYDPQYFHADP
                     DAARGSVFGEVIASGIYTMALWRRLDHQISGDIAWICGVGWEEVKWPTAVRAGDRLRA
                     RYECLSKRPSGSDPKRGVVEFRYTLLNQRGETAWTCRSINLIETGWRA"
     gene            complement(4681..4998)
                     /locus_tag="VD737_09540"
     CDS             complement(4681..4998)
                     /locus_tag="VD737_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXW10848.1"
                     /translation="MAKMVVTDRDGNVHDVDGRPGLKLMETLREYEYGVTAICGGLCS
                     CATCHIFVSPEWMERLPAPQSDEKELLVELQHYDPSTSRLSCQVEFTDALDGLELKIA
                     PDE"
     gene            complement(5019..5957)
                     /locus_tag="VD737_09545"
     CDS             complement(5019..5957)
                     /locus_tag="VD737_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HXW10849.1"
                     /translation="MPEDPGLDPHRAEQAARTALRTDPATAEFAEARFTPVPGGLSNH
                     AWHVVTDRAGEYFVRLARRYGEQLGADHAAECRVLEITGRAGLSPPIVRCDPVARLLV
                     TIWIDAPRVGRPLRSPTALSLVAQALATLHHLRVPRGLRTVRFDEQARALEAAVRPDA
                     VADTLRHRAREVFARLRADDTPPVLCHHDLNPLNLLRDRDGRLWLVDWEYAGLGDAAI
                     DLASFASQHGLDARRREWLLDGYRRSGRTVVPARFELARWAFDYVQWAWYRAALGRPD
                     ARLEPALVDGRVARLAVSLRRRATGLLRCNNAGFAN"
     gene            complement(5950..6555)
                     /gene="pnuC"
                     /locus_tag="VD737_09550"
     CDS             complement(5950..6555)
                     /gene="pnuC"
                     /locus_tag="VD737_09550"
                     /inference="COORDINATES: protein motif:HMM:NF016837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HXW10850.1"
                     /translation="MTDAIVAALAAVTPLEWLAVLLAIAYLLLAIRQNPWCWAFAIMS
                     AALYLVLFARAGLVMQSALQIFFVAMAVYGWRTWRGTATSAPLPVRRWPWPRHLWALG
                     GVGLVTAVNGALITHDGAGWVPYADAGVAWASVFTTWLVARKLLENWLWWIGIDVVAA
                     TLYGTQGLYATAALYLLYAVLAVRGYQSWRRDLPTAEPVHA"
     gene            complement(6557..6814)
                     /locus_tag="VD737_09555"
     CDS             complement(6557..6814)
                     /locus_tag="VD737_09555"
                     /inference="COORDINATES: protein motif:HMM:NF019246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkoF family
                     thiamine/hydroxymethylpyrimidine-binding protein"
                     /protein_id="HXW10851.1"
                     /translation="MRTAVEISLYPLDADYVPPIKDFIERLNAHAGLTVVTNAMSTQV
                     AGEHGVLFAALEQETRRTFAAERRAVFVMKVLGGQPTSDER"
     gene            complement(6842..>8968)
                     /locus_tag="VD737_09560"
     CDS             complement(6842..>8968)
                     /locus_tag="VD737_09560"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXW10852.1"
                     /translation="TFRTRSRFTRAGSRAAGARRLLLLASVATACHAVPLTAAPTETP
                     DAGLEEIVVTASLRNQTATELAASATVLSDATLAAAGLQHFEDVLGLVPNLNWSGGTS
                     RPRFFQMRGIGELEQYQGAPNPSVGFLVDEIDLSGVGMPATLFDAEQVEVLRGPQGTR
                     YGANALAGLVKIKTRDAEASPQLHAEVTAGDLDTWSTGLVAGGALGDRGSAWRLVGQH
                     YESDGFQRNAFLGRDDTNGRDETTLRGKLSLALPAAWRADLAALHVDLDNGYDAFSPD
                     NSRRTLSDRPGRDAQRTHGASAVLSGPLGDLTLSSTSAYARSDSVYSFDGDWGNDAYW
                     GEFAPYDYYTHYARERSTLTEDLRLEGGAGEAVDWAAGVYVLRLTEDSLQRDYFAQDL
                     LRSPLATEYTATSLAGYGEAQWRLGPRVSLTTGMRVETRGADYEDSDGAQFDPRDTMV
                     GGQVSLQGELATGSRWYATLARGYKAGGFNIGQFVPDDRRQFAPEYLWNAELGFKFSA
                     PDEGLQGEVALFHMWREDQQVATSFQLDPGDPLSYVFYTDNAAKGRNYGVEASASWRA
                     TERLTFGASLGLLRSEYLGYRYGDRDLDGREQAHAPGWQYSLSAQWGGDEGWMARADL
                     AGSDAFYFDASHDRRSEPYALLNLKAGYTRGPWSVYAWARNVTDEDYAVRGFYFGLEP
                     PDYANELYVQRGDGRLVGATLQWRWR"
BASE COUNT         1428 a         3086 c         3032 g         1422 t
ORIGIN      
        1 ggcgcgcccc actgcgtgtg cgtccacagc gtcgccaccg actgcgaggc gatgcggtag
       61 cgctcgtcag tcaggttgcg cccgtagccg cgcacgaacc agttgccgtc ggcgcccgtg
      121 taggtcacgg aggcgttgag caggttcttc tcgtcgaggt acgcgtcgta ctcgctgccc
      181 gccgccgaga tgtagaacag gttttcgtcc tcgtggtagt actcggcgcc gaactcgagc
      241 gcggcgccac tgccgagcgt ccacgtgtag agcgcctgga tcgagcccat gaattccggc
      301 gaacgcggca ccggcacccc cgagaagtcc tcgttgaagg cctggatggt cgcaccggtg
      361 actggatcca cgacctcggg ctggttgatc acgaactcct tgtactccgc gtccaggaac
      421 gaggcttgcg cacgcaggcg gaacgcgtcg gtcagcagcg cctggaactc gagctccacg
      481 cccttcgact cgacctccgc ggcgttgtag aacacggttt cctggaacgt ggcgccggcc
      541 gagttgaccg tgatgatgtt gaccgcgcgc tgcgcgtcct cgtacttcgt gaagaacacg
      601 gtcgggttga aacggaagcg gctgtcctcg ctctcccact tcatgccgag ttcgtagttg
      661 gtcgcgaact ccggatcgac cgggcgcgtg agctccgaga ccatcaggcc gctcgtgccg
      721 gtctggtcgt tgtagccacc cgccttgtag ccacgcgaca ccgtcaggta cgagaacagg
      781 ttgtccgtga acttgtacga accgatcacg cgccagctcg gctccgtcca ctcctcggag
      841 aggttctcga atccgggcgt gttcttgttg acgatgatgc cgcccgggta cttgttgaag
      901 ttgccggcct gcagcggatc gttgaagtcc tgccaggaca ggctgccatt gagggtgttg
      961 tcgtcgagta tgtcgaagcc gacctgcgtg cggccggccc actcgcgctc gtccctggtc
     1021 atgcggtagc cgccggtcag cgagaactgg tcggtgacgt cccaggtcac gtcgacgtaa
     1081 cccgccagcg agtcggaatc ctgccggttg cagagcacct ggggcgtact gttgttgagc
     1141 tgctgtggcg cgccgatgct gcccaggtcg aaggccaggt cgatgaagcc caggacctgc
     1201 acgacgcaga acgaggcgtc gttggtctgc tggaacgcgc cggcgacgaa gttgaccggc
     1261 ccgtcgaact tcgacgcata gcgtgcttcg aactgcgtgg tctcgcgcgt cgtgtcgcgc
     1321 gtcgcgtcga acagcgaaat gacctcgccc gtcaggacgt tgaccggcgc ggcgccggtg
     1381 tacgtgttgg gcaggtgttc gtcctggtcg cgcttgcccg ccacggcgta gatcgtgccg
     1441 tggccgaggt cccactccat gttcaggtag acgccgtcga cgtcgatgac ctggcccttg
     1501 cccatcttca gcagcgaatc gttgcgctgc gtggcgccca tgttgtcgag cgggtcgccg
     1561 ctcgggcgcg tgaagcccag gacgttccag aggtacgggc cgtccggcgg cgtgtcgttg
     1621 tacgcgggca cggcgtccga ccggtcgcgc atgatttcgt actgcgccag gaaggtgagg
     1681 tcttccgtcg gctgccactg ggccttgatg cgtccgttga cgatgtcgtc gccgcccgcg
     1741 ctcttgcccg gctggccgcc gcccgactgg cccgtgacgc cgtgaatcgt gaacggcgcg
     1801 aacgtgccgc cgaagatgtt gagcgtattg atcgggccgt agtcggcgcc gagcttgtag
     1861 tagccgtcgg acttcgaata ggcgccgacg aagcgcatcg cgaacacgtc gtcgacgagc
     1921 ggcaggtcga tcgagccctg cacgcgctcc gaattgaagc tgccgtaggc cagccggaag
     1981 tcggcaccga attcgtccat gtcgggccgc ttggtctgga tcgtgaccgc gccacccgtg
     2041 gtgttcttgc cgaacagcgt gccctgcggg ccgcgcagca cctcgacgct gtaggtgtcg
     2101 aacgtgtcga gcaactgggt ctgcacgctc ggcatgacga agtcgtcgac ctgcacgctc
     2161 acgggcgcgt cctggtagac gatgatgtcg gtcacgccga cgccgcgcat cgcgaacgac
     2221 gcggcgttga aggcggtgac ccgtgccgcc gagaaacccg gcaccgacga ggcgatgccg
     2281 gagatgtcgc gggcgatcat gcggtcgatg tcctcggtcg tcacggcgga caccgaaacg
     2341 ggtgtctgct gcaggttcgt gacgcccgcg cgcgtcgcgg tcaccagcac ttcctccaag
     2401 ccaccggcgg cggtcgtcgg ctcttccggc gcctgggcgt gcaacgccgc gggcgcggcg
     2461 gcggcgagca cggcggaaac gatgcgggcg agcgacgtgg agcgcggtcg gtacacggac
     2521 atggacggtt tcccccggag tggtcaggcc cgttgcgcgg cccaggtgtt ggagcagtgt
     2581 tcgatgctga ttcgcagctc agcggaacgg agttttaatc cacccccaaa aaaaaatcaa
     2641 acatgactgt ttttcattct tgcaacgcaa aaagaaacga tcgcggcggc gttgtgcggg
     2701 aaaacaagcg ctgtcgaacc gcgcggtcgg cgctgtcggg ttttcgcaac cgaccggccg
     2761 cacgactcgg gccgcttaaa acagtcagtt ctgttttttt attcgggtgg cgctatagtc
     2821 ggtcgccccg ctgtctttcg cgtcaccgtt cgaggatctg tcgatggctg cgaacctgaa
     2881 gcccgcctgg cccatgtccc tgagcgcccc cgtcgattcg ctcgaagcca agcaacctta
     2941 cgtggacgtc ggccacgacg tggtcgaccc gtcgcgctac tactcgcgcg agttcatgga
     3001 gctcgagtgg aagttcctgt ggccgcgcgt gtggttgctc gcgggcgtca cgtcggacgt
     3061 acccgaggaa ggcgactaca cggtcttcaa ccacggacac gaaagcatcg tgatcgtgcg
     3121 ccaggaggac ggcggcctgc gggccttctt caacgcctgc ccgcaccgcg gcaatccgat
     3181 cgcgctcaac gagcgcggca gcgtcgcgaa gttcacgtgc gcgttccacg cgtggcgctt
     3241 ctgtctggac ggcaagctcg actgcatcac cgacgagcac agcttcgacc gtggcctcgt
     3301 cgcgcaccgg ccggggctca ccgaggtgcg ctgcgaccag gtcggcgggc tgatcttcgt
     3361 caacatggac ggcaaggcgc cgcccctgcg cgagtggctc ggcctgccgc cgggttatat
     3421 cgagaattac cagatcgacc ggatgcacgt cgtgcggcac gtgcgcacgg agtggttcgc
     3481 caactggaag accggcgtcg acgcgttcta cgagacgtac cacctgccgc acatccatcc
     3541 gcagacgcag tgcgtgatgg aggactactc gcagtacgac cagtacccga acggcttcac
     3601 gcgcatgatc gtgccgatct gcacgccgag ccaccgggtg atcgaccgcg actccgtcaa
     3661 cgacgggctg aagtacatga tgctcgaggc cgggatggac ccggccgcct tccagggcac
     3721 gggcatcgag gtgcgcaccg ccgtgcagaa agccaagcgc gagcgcgcga agcggctcgg
     3781 gctcgaccac tacgacaagt ttaccgacgg ccagctcacc gacagctggg ccaccggtta
     3841 cttcccgaac gtgcagatcg gcatgcaccc cgagggcgtg ttcatcatgc ggttccagcc
     3901 gcacccgacc gaccccgagc gtttcttcta cgacaacatg acgctgatcc gctacgtcga
     3961 cgacccgaac tacagcgtgc ccggctggat ggggctgccc aaaggcaccg acctcaccgg
     4021 cgccacgcgc ccgaacatcg agcaccacgc cgccggcgtc cgcgccgacc tcggcgaagt
     4081 gctgaaccag gacgtcgacc tggtcgaggc ggtgcagcgc ggccagaagt cgcgcggctt
     4141 ccgtggaccg ctgtggagcg agcaggagat ccggctccgc cactaccatc acgagatcga
     4201 ccgctacctc cgcggcgaga agtgaccgtc cgccgctgct acgacgacct ggtcgtcggc
     4261 gaggtccgtg agtccggcga gcacgtcgtc gaactggacg ctctcgtcgc gttcgcgcgc
     4321 gagtacgacc cgcagtactt ccacgccgat ccggacgccg cgcgcggctc ggtcttcggc
     4381 gaggtgatcg cgtccggcat ctacacgatg gcgctgtggc gccggctcga ccaccagatc
     4441 agcggcgaca tcgcctggat ctgcggcgtg ggctgggaag aggtcaagtg gccgacggcc
     4501 gtgcgagcag gagatcgcct gcgcgcgcgc tacgaatgcc tgtcgaagcg cccatcgggg
     4561 tcagacccga agcgcggcgt ggtcgagttc cgttacacct tgctgaacca gcgcggcgaa
     4621 actgcctgga cctgccgcag catcaacctg atcgagacgg gctggcgggc ctaggcccgc
     4681 tcattcatcg ggcgcgatct tgagctcgag cccgtcgagc gcgtcggtga actcgacctg
     4741 gcagctcagg cgcgaggtcg acgggtcgta gtgctgcagt tcgaccagca gttccttctc
     4801 gtcgctctgc ggcgccggca gccgctccat ccactccggc gacacgaaga tgtgacaggt
     4861 cgcgcatgag cacagcccgc cgcagatcgc ggtcacgccg tactcgtatt cgcgcagcgt
     4921 ctccatgagc ttgagcccgg gacgtccgtc gacgtcgtgg acgttgccgt cgcggtcggt
     4981 caccaccatc ttcgccattg catgacaccc gtcgaacgct agttcgcaaa ccccgcatta
     5041 ttgcagcgca agaggccagt tgcacgtcgg cgcaacgaca ccgcgagccg cgcgacgcga
     5101 ccgtcgacga gcgcaggttc gaggcgcgcg tccggccgcc cgagcgccgc gcggtaccac
     5161 gcccactgta cgtaatcgaa cgcccagcgt gcgagctcga agcgtgccgg caccaccgtc
     5221 cggccggacc gtcgatagcc atcgagcaac cactcgcgcc gacgcgcgtc aaggccgtgc
     5281 tggctcgcga acgacgccag gtcgatcgcc gcgtcgccga ggcccgcgta ctcccagtcg
     5341 accagccaga gccggccgtc gcggtcgcgc agcaggttca gcgggttcag gtcgtggtgg
     5401 cacagcacgg gcggcgtgtc gtccgcacgc agccgtgcga acacctcacg cgcgcgatgc
     5461 cgcagggtgt cggcgactgc gtccggccgt acggccgctt cgagcgctcg cgcctgctca
     5521 tcgaaccgca ccgtccgcag accgcgcggc acccggaggt ggtgcagcgt cgcgagcgcc
     5581 tgcgcgacga gcgacagggc cgtggggctg cgcagcggcc ggcccacgcg cggcgcatcg
     5641 atccagatcg tcaccagcag ccgcgcgacg ggatcgcagc gcacgatcgg cggcgacagc
     5701 ccggcgcgcc cggtgatctc cagcactcgg cattcggcgg catggtccgc accgagctgc
     5761 tcgccgtagc gacgcgccag gcgcacgaag tactcccccg cccggtcggt cacgacgtgc
     5821 caggcgtggt tcgaaagtcc gcctggcacc ggagtgaagc gggcctcggc gaactccgcc
     5881 gtcgcagggt ccgtgcgcag cgcggtgcgc gccgcctgct cggcacggtg cgggtccaac
     5941 cccgggtcct caggcatgca ccggctcggc cgtcggcagg tcacgccgcc agctctggta
     6001 tccacgcacc gcgagcaccg cgtagagcag gtacagcgcc gccgtggcgt agaggccctg
     6061 ggtgccgtac agcgtcgccg ccacgacgtc gatcccgatc caccacagcc agttctcgag
     6121 cagcttgcgc gcgacgagcc acgtcgtgaa tacgctcgcc cacgcgacgc cagcgtccgc
     6181 gtagggcacc cagccggcac cgtcgtgcgt gatcagcgcg ccgttcaccg cggtgaccag
     6241 gccgacgccg ccgagggccc agagatgtcg cggccacggc cagcgccgca ccggcaacgg
     6301 cgcgctcgtg gccgtgcccc gccacgtccg ccagccatat acggccatgg ccacgaagaa
     6361 gatctgcagc gcggactgca tcaccagccc ggcgcgcgcg aacagcacca ggtacagcgc
     6421 cgcgctcatg atcgcaaacg cccagcacca cgggttctgg cggattgcga gcaacaggta
     6481 cgcgatcgcg agcagcaccg cgagccactc gagcggcgtg accgccgcga gcgccgcgac
     6541 gatcgcgtcc gtcacttcag cgctcgtcgg aggtcggctg gccgcccagc accttcatga
     6601 cgaacacggc gcgccgctcc gccgcgaacg tccgccgcgt ctcctgttcg agcgcggcaa
     6661 acagcacgcc gtgctcgccg gccacctgcg tgctcatcgc attggtgacc accgtgagcc
     6721 ccgcgtgggc gttcaggcgc tcgatgaagt ccttgatcgg cgggacgtag tcggcgtcca
     6781 gtggatagag gctgatttcg acggcagtgc gcatgtcggt ctccggcgtt gcaggatgga
     6841 atcagcgcca gcgccactgc agggtcgcgc cgacgaggcg gccgtcgccg cgctggacgt
     6901 agagctcgtt cgcgtagtcg ggaggctcga ggccgaaata gaagccgcgc accgcgtagt
     6961 cctcgtcggt cacgttgcgg gcccacgcgt agacggacca cgggccgcgc gtgtagcccg
     7021 ccttcaggtt cagcaatgcg tacggctcgg accggcgatc gtggctcgcg tcgaagtaga
     7081 acgcgtcgct gccggcgagg tccgcccgcg ccatccagcc ttcgtcgccg ccccactggg
     7141 ccgacagcga gtactgccag cccggcgcat gagcctgctc gcgcccgtcg aggtcacgat
     7201 cgccgtaccg gtaacccagg tactcggagc gcagcagccc gagcgaggcg ccgaacgtga
     7261 gccgttcggt ggctcgccac gaggccgagg cctctacgcc gtaatttcgt cccttcgcgg
     7321 cgttgtcggt gtagaacacg tacgacaacg ggtcgcccgg gtcgagctgg aacgacgtcg
     7381 ccacctgctg gtcctcgcgc cacatgtgga acagcgcgac ttcgccctgc aaaccttcgt
     7441 ccggcgcgct gaacttgaaa ccgagttcgg cgttccagag gtactcgggt gcgaactggc
     7501 gccggtcgtc cggcacgaac tggccgatgt tgaagccgcc cgccttgtag ccgcgcgcga
     7561 gcgtcgcgta ccagcgcgac ccggtcgcca gttcgccctg cagcgacacc tggccgccga
     7621 ccatcgtgtc gcgcggatcg aactgcgcgc cgtcggaatc ctcgtaatcg gcgccgcgcg
     7681 tttcgacccg catgcccgtg gtgagcgaga cccgcgggcc gagacgccat tgcgcctcgc
     7741 cgtagcccgc gagactggtg gccgtgtact ccgtcgcgag cggagaccgc agcaggtcct
     7801 gcgcgaagta gtcgcgctgc aacgagtcct cagtgagccg cagcacgtac acgccggccg
     7861 cccagtcgac cgcctcgccc gcgccgcctt ccagccgcag gtcctcggtc agcgtcgagc
     7921 gttcgcgtgc gtagtgcgtg tagtaatcgt agggcgcgaa ctcgccccag taggcgtcgt
     7981 tgccccagtc gccgtcgaag ctgtagacgc tgtcggagcg tgcgtaggcg ctcgtgctgc
     8041 tcaacgtcag gtcgccgagc ggtccggaga gcacggcgga cgcgccatgc gtgcgctgcg
     8101 cgtcgcgccc cggccggtcg gagagcgtgc ggcgcgagtt gtccgggctg aacgcgtcgt
     8161 agccgttgtc gagatcgacg tggagcgccg ccaggtccgc gcgccatgcg gccggcagcg
     8221 cgagcgaaag cttcccacgc aacgtcgtct cgtcgcgtcc gttggtgtcg tcacgaccga
     8281 gaaacgcgtt gcgctggaag ccgtcgcttt cgtaatgctg cccgacgagc cgccacgccg
     8341 agccgcggtc gccgagtgcg ccacccgcga ccagtccggt cgaccacgtg tcgagatcgc
     8401 cggcggtgac ctcggcgtgc agctgcggcg aggcttccgc atcgcgcgtc ttgatcttga
     8461 ccaggccggc cagcgcattg gcgccgtagc gcgtgccctg cgggccacgc agcacctcga
     8521 cctgctcggc gtcgaacagg gtcgcgggca tgccgacgcc actcaggtcg atctcgtcga
     8581 ccaggaagcc cacggacggg ttgggcgcgc cctggtactg ctcgagttcg ccgatgccgc
     8641 gcatctggaa gaagcgcggc cgcgacgtgc cgcccgacca gttcaggttc ggcacgagtc
     8701 cgagcacgtc ctcgaagtgc tgcagtcccg ccgccgcgag cgtggcgtcg gacagcacgg
     8761 tggcgctggc cgcgagctcg gtggccgtct ggttgcgcag cgacgcggtc accacgatct
     8821 cctcgagccc ggcgtccggc gtctcggtgg gcgccgccgt cagcgggacg gcgtggcacg
     8881 cggtcgcgac gctcgccagg agcagcaggc gacgcgcgcc ggcggcgcgg ctgccggcac
     8941 gagtgaatct ggaacgagta cgaaaggt
//
LOCUS       DASPFR010000361         5859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1816131, whole genome shotgun sequence.
ACCESSION   DASPFR010000361 DASPFR010000000
VERSION     DASPFR010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816131"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VD737_09565"
     CDS             <1..328
                     /locus_tag="VD737_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10853.1"
                     /translation="ERGDTRCRFHREIDMQRTLVLVTLALFGALSAVALWQHGYVGLF
                     EQAFASWAGLQVLVDLAIALTLVMVWMWRDARATGRNVWPWLVATLALGSFGPLGYLL
                     TRARSA"
     gene            436..1035
                     /locus_tag="VD737_09570"
     CDS             436..1035
                     /locus_tag="VD737_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HXW10854.1"
                     /translation="MAALVFPLLALASAGCGGPFTSAANGVTAASPPPRTNPTPPSRA
                     APGEPAALVGITAAHNRVRARVGVPPLTWNPALAEVAQRWASACVDRQAPRGMIDHST
                     GESPAFGGPLGENLHATTGPAVDPTGAVEGWAAEARDYDYATDTCRRGAMCGHYTQVV
                     WRTTREVGCAVSTCPRLRYRTSLVCNYFPAGNWEGQRPY"
     gene            complement(1072..1467)
                     /locus_tag="VD737_09575"
     CDS             complement(1072..1467)
                     /locus_tag="VD737_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10855.1"
                     /translation="MAANAPTDPRMDADDLYREDVYSDRKVGTIRVMTPVKRDGSPDP
                     TRAAVYVGQAQIMTPAGVLPLSFEIEAKSLTEACAGFADGAKVAFEETMKELQEMRRQ
                     QASSIVIPDAGAAASLGQGGPPRGKIQFP"
     gene            complement(1523..1738)
                     /locus_tag="VD737_09580"
     CDS             complement(1523..1738)
                     /locus_tag="VD737_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10856.1"
                     /translation="MIRWFLILALLQLVAFWKILGRMGYPPWLAIMASIPLVNLVLLY
                     YVALTPWPREGSWNDGTGPSGQGDAKF"
     gene            complement(1848..2345)
                     /locus_tag="VD737_09585"
     CDS             complement(1848..2345)
                     /locus_tag="VD737_09585"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXW10857.1"
                     /translation="MDLPSSFRSLNTLAARIAAIAVDPALLLLRVWVSWQFLKSGWLK
                     LADWETTRFLFTEEYHVPWLPPTLAAALGTFGEVAFPALLVVGILGRYAALGLAAVNV
                     MAVVAYAHVLYAAGYEGALAQHWLWGLALAVLVVFGPGAWSVDGWLARSKDAAVSAAL
                     TPARR"
     gene            complement(2396..2671)
                     /locus_tag="VD737_09590"
     CDS             complement(2396..2671)
                     /locus_tag="VD737_09590"
                     /inference="COORDINATES: protein motif:HMM:NF021559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HXW10858.1"
                     /translation="MSIRNHAIATALGSLLALGGATASAGDMDKAASDKEKCYGVAKA
                     GKNDCATSSHACAGHSKVDNDPVEWKYVPKGECEKMGGTPKAAATKS"
     gene            2870..3382
                     /locus_tag="VD737_09595"
     CDS             2870..3382
                     /locus_tag="VD737_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXW10859.1"
                     /translation="MDPRRRRFEQLCEPLRADVYRFAFWLCRDRSIAEDVVQETLLRA
                     WRSIESLEEDGKARAWLLTIARREHARLHERKRLETVDVDALAANEDPALGLGDGDDG
                     RLGDVRAAIGGLPAEYREPLVLQVLMGYSTDEIGAQLGLTAGAVCTRLSRARQKLRSA
                     LLDAARGGRP"
     gene            3379..4068
                     /locus_tag="VD737_09600"
     CDS             3379..4068
                     /locus_tag="VD737_09600"
                     /inference="COORDINATES: protein motif:HMM:NF023287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3379 family protein"
                     /protein_id="HXW10860.1"
                     /translation="MNCDEARFALEADPASPDVALAAHVRACPDCAAYRTELFELDGR
                     LRAALQVPVPPSALQPRVALPIAAPRPRWTTQFALAASIGAVALLVGLLWSAFPRAAL
                     ATDVTAHMAHEPAAWTTTAALSDARVAATLERRGLHLRPGVVDVTYVQTCWFRGHRVP
                     HLVVAGAGGPVTVMVLTAEPVTSRRSFDERGYRGVLVPAERGALAVLARDGDDVDVDA
                     VAARALEALQY"
     gene            4107..5777
                     /gene="hutU"
                     /locus_tag="VD737_09605"
     CDS             4107..5777
                     /gene="hutU"
                     /locus_tag="VD737_09605"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HXW10861.1"
                     /translation="MNDLHRSRTGRGPIRAPRGTALSARSWLTEAPLRMLMNNLDPEV
                     AERPEDLVVYGGIGRAARNWDAYERIVRCLRDLRDDETLLVQSGKPVGVFRTHVDAPR
                     VLIANSNLVGRWSTWEHFHDLDRKGLMMYGQMTAGSWIYIGSQGIVQGTYETFVEMGR
                     QHYGGDLAGRWILTAGLGGMGGAQPLAATMAGASMLAIECRTERIQKRLDTRYLDVRA
                     DSLDHALELIDRSVRERRPLSVGLLGNACEVLPELLRRGVRPDAVTDQTSAHDPVNGY
                     LPEGWTVEQWEHASDPRAVEQAARRSMRKHVEAMLEFRRHGAPVFDYGNNIRQVAYDA
                     GEARAFDFPGFVPAYVRPLFCRGVGPFRWAALSGNPEDIYRTDAKVRELIPDDPHLHR
                     WLDMARERIQFQGLPARICWVGLGQRHRLGLAFNEMVARGELEAPVVIGRDHLDSGSV
                     ASPNRETESMRDGSDAVSDWALLNLLLNCASGATWVSFHHGGGVGMGYSQHAGQVIVA
                     DGTPEAARRLERVLTTDPGMGVVRHADAGYEIAVDAAKRHGIKMPMLK"
BASE COUNT          844 a         2051 c         2084 g          880 t
ORIGIN      
        1 ggagcgcggc gacactcgat gccggtttca ccgggagatc gacatgcaac gcaccctcgt
       61 tctcgtcacg ctcgcactct tcggcgccct gagcgccgtc gcgctctggc agcacggcta
      121 cgtgggcctg ttcgaacagg cgttcgcgag ctgggccggg ctgcaggtgc tcgtggacct
      181 cgcgatcgcg ctcacgctcg tgatggtctg gatgtggcgc gatgcccgtg ccacgggtcg
      241 caacgtctgg ccgtggctgg tcgcgacgct cgcgctgggc tcgttcgggc cgctcggtta
      301 tctgctgacg cgggcgcggt ccgcttgacg gacgccgcga ttcgccgggc accgaccggc
      361 gagtgtctgc tcgcctgcct tgtggccggc gggccggttc ggctcactgc ggccatgccg
      421 tcccgacgac gaccgttggc cgcactggtc ttcccgttgc tggcgctcgc gagcgcgggc
      481 tgcggagggc ctttcacctc ggccgccaac ggcgtgacgg ccgcgtcgcc gcccccgagg
      541 acgaacccca cgccgccgtc gcgcgccgca cccggcgagc ccgcggcact ggtcggcatc
      601 acggcggccc acaaccgcgt gcgggcgcgg gtcggcgtgc cgccgctcac ctggaatccg
      661 gcgctcgccg aggtcgcgca gcgctgggcc agtgcctgcg tggaccgcca ggccccgcgc
      721 ggcatgatcg atcacagcac cggcgaaagc ccggcgttcg ggggcccgct cggcgagaac
      781 ctgcatgcca cgacgggccc cgccgtcgac ccgaccggcg cggtcgaggg ctgggccgcc
      841 gaggccaggg actacgacta cgccaccgac acgtgtcgcc gtggcgcgat gtgcggccac
      901 tacacccagg tggtgtggcg aacgacccgc gaggtcggct gcgcggtgag cacatgcccg
      961 cgcctgcggt accgcacctc gctcgtctgc aattattttc cggcgggcaa ctgggaaggc
     1021 cagcggcctt actgagcgac tcgcgcggcg ctcagcgccc ctggaccggc gtcacgggaa
     1081 ctggatcttg ccgcgtggcg gcccgccctg gccgagcgag gccgcagcgc ctgcgtccgg
     1141 aatcacgatc gacgacgcct gctggcgacg catctcctgg agttccttca tcgtctcctc
     1201 gaaggccacc ttcgcgccgt cggcaaaccc ggcacaggct tcggtcaggc tctttgcctc
     1261 gatctcgaac gagagcggca gcacgcccgc gggcgtcatg atctgcgcct gcccgacgta
     1321 gaccgcggcg cgcgtcgggt ccggcgagcc gtcgcgcttg acgggggtca tcacgcggat
     1381 cgtgcccacc ttgcggtctg agtacacgtc ttcgcggtac aggtcgtccg cgtccatcct
     1441 cgggtcggtg ggggcgttgg ctgccatggt cgtcctctaa gcgtcaaacg gcgcgcagag
     1501 acctgcgcgg gccgcaaagt tatcagaatt tggcgtcgcc ttggccggac gggccagtgc
     1561 cgtcgttcca gctgccttcg cgtggccacg gcgtgagcgc gacgtagtac agcagtacga
     1621 ggttcacgag cgggatcgac gccatgatcg cgagccacgg cggataaccc atgcgcccga
     1681 ggatcttcca gaacgcgacg agctgcagca gtgcgaggat caggaaccag cggatcatgc
     1741 ggcaccgggc ggcgggtgga agccgtcatc atagcggccg gcgtcgcgtg cgacgccgtt
     1801 ggaacggtcc gctgagctct actgccggcg ttccacgacg cgcgccgtca acgccgtgcc
     1861 ggtgtcagcg ccgcgctcac ggcagcgtcc ttcgatcggg cgagccagcc gtccaccgac
     1921 caggcgcccg gaccgaacac gaccagcacg gcgagcgcga gtccccagag ccagtgctgc
     1981 gcgagcgcgc cctcgtaacc ggccgcgtac agcacgtgcg cataggcaac cacggccatg
     2041 acgttgacgg ccgcgagccc cagcgccgcg taacgaccga gtatcccgac gaccagcagc
     2101 gcgggaaaag ccacttcgcc gaacgtgccg agcgccgccg cgagcgtcgg cggcagccag
     2161 ggcacgtgat actcctccgt gaacaggaac cgggtcgtct cccagtccgc gagcttgagc
     2221 cacccggact tgaggaactg ccacgacacc cacacgcgaa gcaggagcag cgccggatcg
     2281 accgcgatgg cggcgatgcg cgcagccagc gtgttcagcg agcgaaaact ggatggcaga
     2341 tccatggcgc ctccggcagg gagcgacgcc cggacggccc gggcgtcgcg tccgatcacg
     2401 acttcgtggc ggcggccttg ggcgtgccac ccatcttctc gcactcgccc ttcggcacgt
     2461 acttccactc gaccgggtcg ttgtcgacct tggagtggcc ggcgcaggcg tggctgctcg
     2521 tcgcacagtc gttcttgccg gctttcgcga cgccgtagca cttctccttg tccgaggccg
     2581 ccttgtccat gtcgccggcg ctcgccgtgg cgccgccgag cgcgagcagg ctgcccagtg
     2641 ccgtggcgat cgcgtgattg cggatactca tcgtcttctc cttgcgtgtc cttgtcgggg
     2701 tacgacgccc gtgcaccgcc gtcgatgcgc gttgcgcggg gtgtcgttgc agtggaccgg
     2761 gcgaccctgc cgatccttac ggcggcgcgg cgtctgcgca ggtgcccggt atgatgcggt
     2821 gcgccgtaag gaatcggcgt ttcggccggt ccgtttacat gggggtgtcg tggacccgcg
     2881 acgacgtcgc ttcgagcagc tctgcgaacc cctgcgtgcg gacgtgtacc ggttcgcctt
     2941 ctggctctgc cgggaccggt ccatcgccga ggacgtcgtg caggagacac tgttgcgcgc
     3001 gtggcggtcg atcgaatccc tggaagaaga cggcaaggcg cgcgcctggc tgctgacgat
     3061 cgcgcgtcgc gagcacgcgc gcctgcacga acgcaagcgc ctcgagacgg tggacgtgga
     3121 cgcgctcgcc gcgaacgaag atccagcgct cgggcttggc gacggcgacg acggccgcct
     3181 cggcgacgtg cgtgccgcga ttggcgggct gccggctgaa taccgggaac cactggtgct
     3241 gcaggtgctg atgggctact cgaccgacga aatcggcgca caactggggc tgacggcggg
     3301 cgccgtgtgc acacggctct cgcgcgcgcg acagaagctg cgcagcgcat tgctggacgc
     3361 agcgcgcggg ggccggccgt gaactgcgac gaggcgcgat tcgcactcga ggcggatccg
     3421 gccagcccgg acgtggcgct ggcggcacac gtgcgtgcct gccccgactg cgccgcgtac
     3481 cgcaccgagc tgttcgaact ggacggccgc ctgcgggcgg cactgcaggt cccggtccca
     3541 ccgtcggcat tgcagccgcg cgtcgcgctt cccatcgccg cgccgcgtcc acggtggacg
     3601 acgcagttcg cactcgcggc gagtatcggc gcggtggcac tgctggtcgg actgttgtgg
     3661 tcggcctttc cacgggcggc gctcgccacg gacgtcacgg cacacatggc gcacgagccg
     3721 gcggcctgga cgacgaccgc cgcgctgtcc gacgcgcgcg tcgccgcgac gctcgagcga
     3781 cggggcctgc acctgcgccc cggcgtcgtc gacgtgacct acgtgcagac gtgctggttc
     3841 cgcggccacc gcgtgccgca cctggtggtc gcgggcgccg gtggaccggt gaccgtcatg
     3901 gtgctgaccg cggagcccgt cacgtcgcgt cggtccttcg acgagcgggg ttatcggggc
     3961 gtgctcgtgc ccgcggagcg cggcgccctg gcggtgctcg cgcgcgacgg cgatgacgtc
     4021 gatgtcgatg cggtcgcggc gcgcgcgctc gaggcgctgc agtactgagc ggacccggcg
     4081 gccggcggcg gtgatataac gcgcgcatga acgacctgca tcgctcccgc acgggacgcg
     4141 gaccgatccg cgcgccgcgc ggcaccgcgt tgtccgcgcg cagctggctg accgaggcgc
     4201 cgctgcgcat gctcatgaac aacctcgatc ccgaggtggc cgagcggccc gaagacctcg
     4261 tcgtgtacgg tggcatcggc cgcgccgcac gcaactggga cgcctacgaa cgaatcgtgc
     4321 gctgcctgcg cgacctgcgc gacgacgaga cgctgctggt gcagtcgggc aagccggtgg
     4381 gcgtgttccg cacgcatgtc gatgcaccgc gtgtgctgat cgcgaattcg aacctggtcg
     4441 ggcggtggtc gacctgggag cactttcacg acctcgaccg caaggggctg atgatgtacg
     4501 gccagatgac ggccgggtcg tggatctaca tcggcagcca gggcatcgtg cagggcacgt
     4561 acgagacgtt cgtcgaaatg gggcgccagc actacggcgg cgacctcgcg ggacggtgga
     4621 tcctcacggc cggactgggt ggcatgggcg gcgcgcagcc gctcgcggcg acgatggcgg
     4681 gcgcctcgat gctcgccatc gagtgccgga ccgaacggat ccagaagcgc ctcgacacgc
     4741 gctacctgga cgtgcgggcc gattcgctgg accacgccct cgagctgatc gaccgctcgg
     4801 tccgcgagcg caggccgctc tcggtcgggc tgctcggcaa cgcctgcgag gtgttgcccg
     4861 agctgctccg ccgcggcgtg cgacccgatg ccgtgaccga ccagacgtcc gcgcacgacc
     4921 ccgtcaacgg ctacctgccc gagggctgga ccgtcgagca gtgggagcac gcgagcgacc
     4981 cgcgcgccgt cgagcaggcc gcgcgtcgct cgatgcgcaa gcacgtcgag gcgatgctcg
     5041 agttccgccg gcacggcgcc ccggtgttcg actacggcaa caatatccgc caggtcgcct
     5101 acgacgccgg cgaggcgcgc gcgttcgact tcccgggctt cgtgcccgcg tacgtgcgtc
     5161 ccctgttctg tcgcggcgtc gggccgttcc gctgggcggc gttgtccggc aaccccgagg
     5221 acatctaccg cacggacgcg aaagtcaggg aactgatccc cgacgacccg cacctgcacc
     5281 gctggctcga catggcacgc gaacgcatcc agttccaggg actgcccgcg cgcatctgct
     5341 gggtcgggct cggccagcgt caccggctcg ggctcgcgtt caacgagatg gtcgcgcgtg
     5401 gcgagctcga ggcgcccgtc gtcatcggtc gcgatcatct cgactcgggc tcggtggcga
     5461 gccccaaccg cgagaccgag tcgatgcgcg acggcagcga cgcggtgtcg gactgggcgc
     5521 tgctcaacct gctgctcaat tgcgcgagcg gcgcgacctg ggtctcgttc caccatggcg
     5581 gcggagtcgg catgggctac agccagcacg ccgggcaggt catcgtggca gacgggactc
     5641 ccgaggccgc ccgcaggctc gagcgggtcc tgaccaccga cccgggcatg ggcgtcgtcc
     5701 gtcacgcgga cgcgggctac gagatcgccg tcgacgcggc gaagcgtcat gggatcaaaa
     5761 tgccgatgtt gaagtaactc atgaagcaaa gattacagaa aagcagctgc tcgggatcaa
     5821 tcagggcgac ccaccaaatg ggtcgccctg attgttatt
//
LOCUS       DASPFR010000362         3300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_596553, whole genome shotgun sequence.
ACCESSION   DASPFR010000362 DASPFR010000000
VERSION     DASPFR010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596553"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VD737_09610"
     CDS             complement(<1..245)
                     /locus_tag="VD737_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10862.1"
                     /translation="MTAAERAGLDLFERHATALAGAVAACRERGYFTLFPETPDRHRG
                     GAAAMAEGLAAFRAQSGKAFELDQPGTLDHVAGEISP"
     gene            complement(242..730)
                     /locus_tag="VD737_09615"
     CDS             complement(242..730)
                     /locus_tag="VD737_09615"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HXW10863.1"
                     /translation="MEFDRKRLVGTWQLVRWFITYEDGQVTEPLGPAAEGLLVYTDDG
                     WMSAALMAVGRPRLSRRNPRDASTAERAAAFDTFFAYTCRWRVVGDAVEHSVTLSHNP
                     ALVGTVQVRRIRMRGRQLTLSAEEPVADGMRVHRLQWRPAGPAWPQARRRPRRDTAGS
                     AA"
     gene            798..2312
                     /locus_tag="VD737_09620"
     CDS             798..2312
                     /locus_tag="VD737_09620"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HXW10864.1"
                     /translation="MRKYTDINPNDPPDRPTWVDSVDNPYLHGPYTPVVTEVMAVDLD
                     VTAGELPADLCGAYLRQGPNPIFKPPGVYHWFDGDGMVHGVYFRDGRASYVRKWVQTR
                     ALRDDFASGHARWPGMMDKFDVARVSGREDVHMNADYCKDTSNTTLNFHHGKLLSAWY
                     NAGRVYRLDPLTLETEGEEDFAGGLQTSMNAHGKTDPRTGEFINYGYADLQPWLTYYV
                     VGPDGKVRHSTRVETPGPRLPHDTTITLNYTILHDFPLFHDADVLKRTGHRVVQFHRD
                     IPTRFGVMPRYGTQEQVQWFEFEPGYVLHMVNAWEDGDWIVMDGCFQPDPTIRRNPQE
                     GPLASMLAYLRFKGSLRRWRMNLKTGEKSEQQLSDLNTEFCLPDIDRYGTKTRYSYHQ
                     LIPTDLQTSAFEALVKFDHESGSHDIHHYPRGWYPSEASFAPSTKARDEDDGYAVTIA
                     TNAGADYASEAWVYNAKRVADGPIARVRLPARVPAGFHAIWVTGDKLWPQALAA"
     gene            complement(2294..3262)
                     /locus_tag="VD737_09625"
     CDS             complement(2294..3262)
                     /locus_tag="VD737_09625"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXW10865.1"
                     /translation="MTFQQFKKVLAFYGRFTASFTQVGYRWRSLAWPRFVPDFQGQRW
                     LVTGASGGLGGYIALAAARAGAEVVAVARNPAKLDALRSAAGSAAPRVATEACDFALQ
                     SDTERLLQRLQGGRPFDVLVNNVGVLLDDLEVTAEGREASFVTNLLSHYQLTEGLLRR
                     GLLRAPGGCVVNMTSGGGYNVPLSVPMLNVTDPKRYNGVVAYGFHKRAQMVLNQYWRD
                     TYGARGVQFYVMHPGWADTDGVKRSLPRFRKILRPILRNEASGADTALWLAAQRPSQP
                     EQECVWFDRAVRTAHVYERTRATKDSPQSLVAYLERELVQPHAASA"
BASE COUNT          547 a         1173 c         1112 g          468 t
ORIGIN      
        1 tacggcgaga tctcgccagc gacgtggtcc agcgttcccg gctggtccag ctcgaacgcc
       61 ttgccggact gcgcacggaa ggccgcgagt ccctcggcca tggccgccgc accgccgcgg
      121 tgtcggtcgg gcgtctcggg aaacagcgtg aagtagccgc gctcgcggca ggcggcgacg
      181 gcgccggcca gtgcggtcgc gtggcgctcg aacaggtcga ggcccgcacg ttccgccgcg
      241 gtcacgcggc gctcccggcg gtgtcgcgac ggggtcgacg gcgtgcctgc ggccacgccg
      301 gtccggccgg ccgccactgc agccggtgaa cccgcatgcc gtccgcgaca ggttcctcgg
      361 ccgacagcgt caactgccgg ccgcgcatcc ggatgcgacg cacctggacg gtgccgacca
      421 gcgcgggatt gtgcgagagc gtgacggagt gttcgaccgc atcgccgacc acgcgccagc
      481 gacaggtgta ggcgaagaac gtgtcgaacg cggccgcgcg ctccgccgtg gaggcatcac
      541 gcgggttgcg ccgcgacagt cgcgggcgcc cgacggccat gagggccgcg ctcatccagc
      601 cgtcgtccgt gtagaccagc agcccctcgg ccgccggacc gagcggttcg gtgacctgcc
      661 cgtcctcgta ggtgatgaac cagcgcacca gctgccaggt gccgacgagc cgcttgcgat
      721 cgaactccac gcgcgcctcc caatgacttg cattatagta ctatagtccc gcacgcaccg
      781 acccggagcc ccgagccatg cgcaagtaca ccgacatcaa cccgaacgat ccgccagacc
      841 gcccgacctg ggtggacagt gtcgacaacc cgtacctgca cggaccgtac acgccggtgg
      901 tcacggaagt catggccgtc gacctggacg tgacggcggg cgaactgccg gccgacctgt
      961 gcggcgcgta cctgcgccag gggccgaacc cgatcttcaa gccgccgggc gtctaccact
     1021 ggttcgacgg cgacggcatg gtgcacggcg tgtacttccg cgacggccgc gcgagctacg
     1081 tccgcaagtg ggtgcagacg cgcgcgctgc gcgacgactt cgcgagcggt cacgcgcgct
     1141 ggcccggcat gatggacaag ttcgacgtcg cgcgcgtgtc cggtcgcgaa gacgtgcaca
     1201 tgaacgcgga ctactgcaag gacacgtcga acacgacgct caatttccac catggcaagc
     1261 tgctgtcggc gtggtacaac gccggccgcg tctaccggct cgacccgctg acgctcgaga
     1321 ccgaaggcga ggaagacttc gccggcggcc tgcagacttc gatgaacgcg cacggcaaga
     1381 cggacccgcg cacgggcgag ttcatcaatt acggctacgc cgacctgcag ccgtggctca
     1441 cttattacgt cgtcggaccg gacggcaagg tgcgccactc gacgcgtgtc gagaccccgg
     1501 ggccgcgcct gccgcacgac accacgatca cgctcaacta caccatcctg cacgacttcc
     1561 cgctgttcca tgacgcggac gtgctcaagc gcacgggcca ccgtgtcgtg cagttccacc
     1621 gcgacatccc gacgcgcttc ggcgtgatgc cgcgctacgg cacgcaggaa caggtgcagt
     1681 ggttcgagtt cgagccgggc tacgtgctgc acatggtcaa cgcgtgggag gacggcgact
     1741 ggatcgtcat ggacggctgc ttccagccgg acccgaccat ccggcgcaat ccacaggaag
     1801 gaccgttggc ctcgatgctc gcctacctgc gcttcaaggg cagcctgcgc cgctggcgca
     1861 tgaacctgaa gaccggcgag aagagcgaac agcaactctc ggacctcaac accgagttct
     1921 gcctgcccga catcgaccgc tacggcacga agacgcgcta ctcgtatcac cagctgatcc
     1981 cgacggacct gcagacctcg gcgttcgagg cgctcgtcaa gttcgatcac gagtccggct
     2041 cgcacgacat ccatcactac ccgcgtggct ggtacccgag cgaggcctcc tttgcgccga
     2101 gcaccaaggc acgcgacgag gacgacggtt acgccgtgac gatcgcgacc aacgccggcg
     2161 cggactacgc ctccgaagcg tgggtgtaca acgccaagcg cgtggccgac ggcccgatcg
     2221 cgcgcgtccg cctgccggcg cgcgtcccgg ccggctttca cgcgatctgg gtcaccggcg
     2281 acaagctctg gcctcaggcg ctcgccgcgt gaggctgcac gagctcgcgc tcgaggtaag
     2341 cgaccagcga ctgcggtgag tccttcgtcg cgcgcgtgcg ctcgtacacg tgcgccgtgc
     2401 ggaccgcgcg gtcgaaccag acgcactcct gctccggctg cgacggccgt tgcgccgcga
     2461 gccacagggc cgtgtcggcg cccgaagcct cgttgcgcag gatcggccgc aggatcttgc
     2521 gaaagcgcgg cagcgagcgc ttgacgccgt cggtgtcggc ccagccgggg tgcatcacgt
     2581 agaactgcac gccgcgcgca ccgtacgtgt cgcgccagta ctggttgagt accatctgcg
     2641 cgcgcttgtg gaagccgtag gcgaccacgc cgttgtaccg cttcgggtcg gtcacgttca
     2701 gcatgggcac cgacagcgga acgttgtagc cgccgcccga ggtcatgttc acgacgcagc
     2761 cgcccggcgc gcgcagcagg ccgcgtcgca gcaggccctc cgtgagctgg tagtggctca
     2821 gcaggttggt cacgaacgac gcctcgcgtc cttcggcggt gacttcgagg tcgtcgagca
     2881 ggacgcccac gttgttgacc agcacgtcga agggacggcc gccctgcagt cgctgcagca
     2941 gtcgctccgt gtcggactgc agggcgaagt cgcaggcttc ggtcgccacg cgcggcgcgg
     3001 cgctgcccgc ggcggaccgc agggcgtcga gcttggccgg gttgcgggcg acggcgacga
     3061 cctcggcgcc ggcacgcgcg gccgccagcg cgatgtaccc tccgaggccg cccgaggcgc
     3121 cggtcacgag ccaacgctgg ccctggaaat caggcacgaa acgcggccag gcgaggctgc
     3181 gccaccgata tcccacctgc gtgaagctcg ccgtgaagcg accgtagaac gcgagcactt
     3241 tcttgaattg ctggaacgtc atcactgatc tccgcggcgg cggcaggtcg ccgcagcctt
//
LOCUS       DASPFR010000363         4911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_353819, whole genome shotgun sequence.
ACCESSION   DASPFR010000363 DASPFR010000000
VERSION     DASPFR010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353819"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /gene="metL"
                     /locus_tag="VD737_09630"
                     /gene_synonym="metM"
     CDS             <1..851
                     /gene="metL"
                     /locus_tag="VD737_09630"
                     /gene_synonym="metM"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="multifunctional homodimeric enzyme that catalyzes
                     the phosphorylation of aspartate to form
                     aspartyl-4-phosphate as well as conversion of aspartate
                     semialdehyde to homoserine; functions in a number of amino
                     acid biosynthetic pathways; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HXW10866.1"
                     /translation="DHVHAEHYPHAVIIDCSASAEVAKHYAHWLSEGIHVVTPNKKAN
                     SAAYGEYERVKEARRAAGSHYLYEATVGAGLPVIQTLRDLRETGDEIRRIEGMFSGTL
                     AYLFNTWDGRQAFSDVVREARSLGYTEPDPRDDLSGMDVARKLIILAREMGLRTELAE
                     VRVESLVPAALAGCGVDEFVERLAEFDGAMLARLQDAASRGNVLRYVGSVDAQGRAEV
                     GLVELPKTHPFANIALTDNIVRFQTSRYDKNPLIVQGPGAGPAVTAAGVFADLLRVCA
                     YLGAKL"
     gene            848..1840
                     /locus_tag="VD737_09635"
     CDS             848..1840
                     /locus_tag="VD737_09635"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HXW10867.1"
                     /translation="MTATSTPPATAGEVDRRPGEGRTSATAFAPASVGNVGVGFDILG
                     HTVAALGDRVRATRRDEPGVVIRSITGVHVDLPREAAKNTAGMAVLAMVKDLELPYGF
                     ELEIEKGIPLGSGLGGSAASAVAGIVAAAALVDERLDRAQLLKYAMQGEAVASGSVHV
                     DNIAPSLYGGLVLTVGIDNPFVKQIPVPEPVRCVLVHPHMVLATREARAILSQTVPLS
                     DVIWQQANLAGFLAGCFSGDLALIRESLLDVVIEPQRQVLIPGFVEVKQAALAAGALG
                     ASISGAGPTVFAWCDEADAEKVRAGMVEAFARHGLETDSWISTIDRAGARVVGD"
     gene            1840..3150
                     /gene="thrC"
                     /locus_tag="VD737_09640"
     CDS             1840..3150
                     /gene="thrC"
                     /locus_tag="VD737_09640"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXW10868.1"
                     /translation="MQYQSTRDAAHRVGLGEAIARGLAPDGGLYVPTGLPSLPLDAFD
                     GLGDVRAVAKVLLRPFAAGDVLAEWLPAIVDDAFTFPVPVVDVPASAEPLSVLELFHG
                     PTAAFKDFGARFLAATLERLPRAQDPRRLTILVATSGDTGGAVAAAFHRRPWVDVVVL
                     YPRGLVSPRQEKQLACWGANVRTLAVAGSFDDCQRMVKDAFVDPDLARALRLSSANSI
                     NVGRLLPQMAYYAVSSLQLYRRDGRKPNYLVPSGNLGNVAACLWARRVGLPIGDVVLA
                     TNANLTVSDYLRTGEWRPRPSLATLASAMDVGNPSNMERLRWLFGDVDGVRAQFEAVP
                     VGDGEIRTTIRRDHAELAQTWCPHTATAAHVYRQLPPERRQQRWVLVATAHPAKFNEV
                     VEPLISASVPVPAALAELLALPSLQREIEPTLEALREVLMETAA"
     gene            3147..3734
                     /locus_tag="VD737_09645"
     CDS             3147..3734
                     /locus_tag="VD737_09645"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HXW10869.1"
                     /translation="MKGFLGLPLWLWVIFGVAALVAAGYFILEQLRMLRARRAIDNVI
                     SSVAYDELRNVLLPTGSGEQIHVNYLLLTQKGLLLIDLFDAHGVVFAGEKMAQWSIFG
                     PKRHFMIANPLPMLYDRVAAVRQFTGDAVPVEGRVVFSMRGEFPKGRPDSVIRLDELQ
                     DQYPVVEKSPGGAAAAFADVWDRIRTASQPNPLAH"
     gene            3839..>4911
                     /locus_tag="VD737_09650"
     CDS             3839..>4911
                     /locus_tag="VD737_09650"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HXW10870.1"
                     /translation="MGAIGVAPETSGPSHFDEDAWDDLLNYIEERRVIPIVGPDLLRV
                     ETDRGPRLLYDWLAERLAARLSVDPALLPEPLTLNDVVCSYLGQRGRREEAYTRLRSI
                     MREVQFTAPPALRQLAAITDFDLYVTTTFDPLLEQAINAERFGGQPHAEVLAYAPNRV
                     SDLPSERSQLQRVVVYHLLGKLSASPTYVISDEDMLEFICALQSEHLTPEKLFHELEH
                     SHLLLIGSDFSNWLARLFLRMAKRKRLSDPRDVGEILADDHTAKDGRLVAFLQQVSVR
                     TRVYAGAAAFVDELHRRWQQRQVPAGTAAAHAPQRFPPPSREMPENAVFVSYAREDLP
                     AVQRLKAAMDAAGLVTWFDLDRL"
BASE COUNT          708 a         1728 c         1720 g          755 t
ORIGIN      
        1 ccgaccacgt gcacgcggag cactacccgc atgcggtgat catcgactgc tcggcgagcg
       61 ccgaggtcgc gaagcactac gcgcactggc tctccgaggg catccacgtc gtcacgccga
      121 acaagaaggc gaacagcgcg gcctacggcg agtacgagcg cgtcaaggag gcgcgccgcg
      181 cggcgggctc gcattacctg tacgaggcga ccgtgggcgc ggggctgccg gtcatccaga
      241 ccctgcgcga cctgcgggaa accggcgacg agatccgccg catcgagggc atgttctcgg
      301 gcacgctcgc gtacctgttc aacacgtggg acggccggca ggcgttctcg gacgtcgtcc
      361 gtgaggccag gtcgctcggc tacaccgagc ccgacccgcg cgacgacctc tcgggcatgg
      421 acgtcgcgcg caagctgatc atcctggcgc gcgagatggg gctgcgcacc gaactcgccg
      481 aggtccgcgt cgagagcctg gtgcccgcgg cgctcgccgg ttgcggcgtc gacgagttcg
      541 tcgaacggct ggccgaattc gacggggcaa tgctcgcgcg gctgcaggac gccgcgtcgc
      601 gcggcaacgt gctccgttac gtcggttccg tcgacgcgca gggccgggcc gaggtcggcc
      661 tggtcgagct gccgaagacg cacccgttcg ccaacatcgc gctcaccgac aacatcgtgc
      721 gcttccagac ctcgcgctac gacaagaacc cgctgatcgt gcaggggccc ggcgccggcc
      781 ccgccgtcac ggccgccggc gtcttcgccg acctgctgcg cgtctgcgcg tacctggggg
      841 cgaagctgtg accgccactt ccacccctcc cgcgacggcg ggagaggtcg accgcaggcc
      901 gggtgaaggc aggacgtccg ccaccgcgtt cgcgcccgca agcgtcggca acgtcggcgt
      961 cggcttcgac atcctcgggc acaccgtcgc ggcgctcggc gaccgcgtgc gcgcgacgcg
     1021 ccgcgacgag cccggcgtcg tgatccgctc gatcacgggc gtgcacgtcg acctgccgcg
     1081 cgaggctgcg aagaacaccg cgggcatggc ggtgctcgcg atggtgaagg acctcgagct
     1141 gccctacggc ttcgaactcg agatcgagaa gggcattccg ctcggttcgg ggctcggcgg
     1201 ctccgccgca tcggccgtgg ccgggatcgt ggcagcggcc gcgctcgtcg acgagcgcct
     1261 cgaccgggcg cagctgctca agtacgcgat gcagggcgaa gcggtggcga gtggctcggt
     1321 ccacgtcgac aacatcgcgc cctcgctgta cggcgggctg gtgctgacgg tcggcatcga
     1381 caacccgttc gtgaagcaga ttccggtgcc cgagccggtg cgctgcgtcc tggtgcatcc
     1441 ccacatggtg ctcgcgacgc gcgaggcgcg cgcgatcctg agccagacgg tgcccctgtc
     1501 ggacgtgatc tggcagcagg ccaacctggc gggcttcctc gccggctgtt tcagcggcga
     1561 cctggcgctg atccgcgagt cgctgctcga cgtcgtcatc gagccgcagc gacaggtgct
     1621 gatcccgggc ttcgtcgagg tgaaacaggc ggcgctggcg gccggtgccc tcggcgcgtc
     1681 gatctcgggc gcgggcccga ccgtgttcgc gtggtgcgac gaggccgacg ccgagaaggt
     1741 gcgcgccggc atggtcgagg ccttcgcgcg acacgggctc gagacggaca gctggatctc
     1801 gaccatcgac cgcgccggcg cgcgcgtcgt cggcgactga tgcagtacca gagcacgcgc
     1861 gacgccgcgc accgcgtcgg cctgggcgag gcgatcgcgc gagggctggc gccggacggc
     1921 gggctctacg tgcccaccgg cttgccgtcc ctgccactcg acgcgttcga cggcctcggc
     1981 gacgtgcgcg cggtcgcgaa ggtgctgctg cggcccttcg ctgccggaga cgtcctcgcc
     2041 gaatggctgc cggcgatcgt cgacgacgca ttcacgtttc cggttccggt cgtcgacgtc
     2101 ccggccagcg ccgaacctct gtcggtgctg gagctcttcc acggtccgac cgcggcgttc
     2161 aaggattttg gcgcgcgttt cctggccgcg acgctcgaac gcctgccgcg cgcgcaggat
     2221 ccacggcggc tgacgatcct ggtcgcgacg tcgggagata ccggcggggc cgtcgccgcg
     2281 gcctttcatc ggcggccgtg ggtcgacgtg gtcgtgctgt acccgcgcgg gctcgtgtca
     2341 ccgcgccagg agaagcagct ggcctgctgg ggcgccaacg tgcgcacgct cgccgtggcc
     2401 gggtcgttcg acgactgcca gcgcatggtc aaggatgctt tcgtcgaccc cgatctcgcg
     2461 cgggcgctgc ggctctcgtc cgccaacagc atcaacgtcg gtcgcctgtt gccgcagatg
     2521 gcgtactacg ccgtgtcgag cctgcagctg taccgccgcg acgggcgcaa gcccaactac
     2581 ctcgtgccgt ccggcaacct cggcaacgtc gcggcatgcc tctgggcgcg tcgcgtcggc
     2641 ctgccgatcg gcgacgtggt gctggcgacc aatgccaacc tgacggtgtc cgactacctg
     2701 cgcacgggcg agtggcggcc gcggcccagc ctcgctacgc tcgcctccgc catggacgtc
     2761 ggcaacccga gcaacatgga gcggctgcgc tggctgttcg gcgacgtcga cggcgtgcgt
     2821 gcgcagttcg aggcggtgcc cgtcggcgac ggcgagatcc gcaccacgat ccggcgcgac
     2881 cacgccgaac tcgcgcagac ctggtgcccg cacacggcca cggcggctca cgtgtaccgg
     2941 cagctgccgc ccgagcgacg gcagcaacgc tgggtgctcg tcgccaccgc gcatcccgcc
     3001 aagttcaacg aggtggtgga gccgttgatc tcggcgtccg tgccggtgcc ggccgcgctg
     3061 gcagaactgc tcgcgttgcc ttcgttgcag cgtgagatcg agcctacgct ggaggccctg
     3121 cgggaggtgc tgatggagac cgctgcttga aaggcttctt gggactgccg ttgtggcttt
     3181 gggtgatttt cggcgtcgcc gcactcgtgg ccgccgggta tttcatcctc gagcaactgc
     3241 ggatgctgcg cgcgaggcgc gcaatcgaca acgtcatctc gtcggtggcc tacgacgaac
     3301 tgcgcaacgt gctgctgccg accggaagcg gcgagcagat ccacgtgaac tacctgctgc
     3361 tcacgcagaa aggcctgctg ctgatcgatc tgttcgacgc acacggcgtg gtctttgccg
     3421 gcgagaagat ggcgcagtgg tcgatcttcg ggccgaagcg gcacttcatg atcgcgaacc
     3481 cgctgccgat gctctacgac cgtgtcgccg cggtccggca gttcacgggc gacgccgtgc
     3541 ccgtcgaggg ccgggtcgtg ttctcgatgc gcggcgagtt cccgaagggc cgccccgaca
     3601 gcgtgatccg tctcgacgag ctccaggacc agtacccggt cgtcgagaag tcgccgggcg
     3661 gcgccgcggc cgcattcgcc gacgtctggg accgcatccg cacggcttcg cagccgaacc
     3721 cgctggcgca ctgaaaaagc caaggtttgc gcttttcacc ccgcgtgccg ctagactgca
     3781 gcaaagcagg ggggagccgg gacatgcact cggtcgaccg acggcgggtg agacgctgat
     3841 gggcgccatt ggtgttgccc cggaaacctc gggtccgtcg cacttcgacg aagacgcctg
     3901 ggacgatctc ctcaactaca tcgaagagcg ccgggtcatt cccatcgtcg ggcccgacct
     3961 gctgcgcgtg gagaccgacc gcggtccacg gctgctctac gactggctcg cggaacggct
     4021 cgccgcgcgc ctctcggtcg atccggcgct cctgccggag ccgctcacgc tgaacgacgt
     4081 ggtctgcagc tacctgggcc agcgcggccg gcgcgaagag gcgtataccc ggttgcgttc
     4141 gatcatgcgc gaagtgcagt tcacggcgcc gcccgcgctg cgccagctgg cggcgatcac
     4201 ggacttcgac ctctacgtca ccaccacgtt cgatccgctg ctcgagcagg cgatcaacgc
     4261 cgagcgtttc ggcggtcagc ctcacgcgga agtgcttgcc tacgcgccga accgcgtgtc
     4321 ggacctgccg tccgagcgca gccagttgca gcgcgtggtg gtctaccacc tgctcggcaa
     4381 gctctcggcg tcgccgacgt acgtgatctc ggacgaggac atgctcgagt tcatctgcgc
     4441 gctgcagtcc gagcacctga cgcccgagaa gctgttccac gaactcgagc acagccacct
     4501 gctgctgatc ggcagcgact tctcgaactg gctcgcgcgg ctgttcctgc gcatggcgaa
     4561 gcgcaagcgc ctctccgacc cgcgcgacgt cggcgagatc ctcgccgacg accacaccgc
     4621 gaaggacggc cggctggtcg cgttcctgca gcaggtgagc gtgcgcacgc gcgtctacgc
     4681 cggcgccgct gcgttcgtcg acgaactgca ccggcgctgg cagcaacggc aggtgccggc
     4741 cgggacggcg gccgcgcacg cgccgcagcg attcccgccg ccctcgcgcg agatgccgga
     4801 gaacgcggtc ttcgtgagct atgcccgcga agacctgccg gccgtgcagc ggttgaaggc
     4861 agcgatggac gcggcgggcc tcgtcacgtg gttcgacctc gaccggctcg a
//
LOCUS       DASPFR010000364         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1451255, whole genome shotgun sequence.
ACCESSION   DASPFR010000364 DASPFR010000000
VERSION     DASPFR010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451255"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="VD737_09655"
     CDS             complement(<1..1107)
                     /locus_tag="VD737_09655"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXW10871.1"
                     /translation="MKHDGAQPTVEAIYRTESRAVLATLIRLLGDFDLAEEALHEAFR
                     AALERWPRDGVPANPRAWLVSAGRFKAIDQLRRQARFEPYDGERHDRVDEAGDPSARA
                     EESDAVQDDRLRLIFTCCHPALAPDAQVALTLREVCGLTTEEIAAAYLTPAPTLAQRI
                     VRAKAKIRDARIPYEVPAPNQLAARLEPVLRTIYLVFNEGYAASSGAELTRRDLTAEA
                     IRLGRLLVDLLPEPEAMGLLALMLLHDSRRLTRTGTDGEIVLLADQDRSRWDHAQIAE
                     GTRWVERALATRRIGPYTLQAAIAAVHAQAPSAEATDWREIVGLYDALLRLEPSPIVE
                     LNRAVAVAMRDGPAAGLALVDALLERGELRDYRLA"
     gene            complement(1133..1405)
                     /locus_tag="VD737_09660"
     CDS             complement(1133..1405)
                     /locus_tag="VD737_09660"
                     /inference="COORDINATES: protein motif:HMM:NF018500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HXW10872.1"
                     /translation="MDECHDADPRDRPPPIEVPHAELSAAALRGVVEAFVLREGTDYG
                     ERDVEHDTKVGQVMRQLERGEARILFDPVTETVDIVVTRPMARRDA"
     gene            1455..>2773
                     /locus_tag="VD737_09665"
     CDS             1455..>2773
                     /locus_tag="VD737_09665"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HXW10873.1"
                     /translation="MHHIKRSISSRPARVQRTTCLVVACSLLLPGAYAATPSFPEAPY
                     RKHVEVLSSDAFQGRAPGTEGEKKTVEYIEQQFAAAGLSGAIEGSFRQAVPVTEITPH
                     PDEALRVRGSDGRSLDLATLDDVIVWTRRPVPESQIRDAQVVFAGYGIVAPEYGWNDY
                     AGLDVRGKLVLALVNDPGYATQDPKLFTGNAMTYYGRWTYKYEEAVRQGAAGLLVVHE
                     TKAAGYPWDVPRSGATQAQFDLRLADFESQRLPIEGWITEAAAARVLSLAGQDYAALK
                     KASSSRGFRGKALDLRASVGVRNDVQHKTSYNVVGVLRGRTRPDEAFVYTAHWDHLGV
                     KGDAKPGEDAIYNGAQDNATGIAALIELARAFAATRPRPQRSLLFVAVTAEESGLLGS
                     EYFIAHPPVPASRIAGGLNMDNLYAVGPTRDVTVIGFGKSELEDELRR"
BASE COUNT          406 a          966 c          977 g          424 t
ORIGIN      
        1 cgcgagccgg tagtcccgaa gttcgcctcg ctcgagcaac gcatcgacga gcgcaaggcc
       61 ggcggcggga ccgtcgcgca tcgcgacggc cacggcgcga ttcaactcga cgatcggcga
      121 cggctcgagg cgcagcaacg cgtcgtagag cccgacgatc tcacgccagt ccgtcgcctc
      181 ggcgctgggt gcctgcgcgt gcacggcggc gatcgcggcc tgcagcgtgt atggaccgat
      241 ccggcgcgtg gccagcgcac gctcgaccca gcgcgtcccc tcggcgatct gcgcgtggtc
      301 ccagcgtgag cggtcctggt ccgccagcag cacgatctcg ccgtccgtgc cggtgcgtgt
      361 caggcgtcgc gaatcgtgga gcagcatcag cgcgagcagg cccatcgcct ccggttcggg
      421 cagcaggtcg accagcagtc gcccgaggcg gatcgcctcg gcggtcaggt cgcggcgcgt
      481 cagctccgcg cctgaactgg cggcgtagcc ttcgttgaac acgaggtaga tggttcgcaa
      541 taccggctcg aggcgcgccg ccagctgatt cggcgcgggc acctcgtacg ggatgcgcgc
      601 gtcgcggatc ttcgccttgg cgcgcacgat gcgctgcgcc agcgtcggcg ccggcgtgag
      661 gtacgccgcg gcaatttcct cggtggtgag accgcacact tcgcgcagcg tgagtgccac
      721 ctgcgcgtcg ggcgcgaggg cgggatggca gcacgtgaag atcagccgca gtcgatcgtc
      781 ctgcacggcg tcggattcct cggcgcgggc gctcgggtcg cccgcttcgt cgacgcggtc
      841 gtgacgctca ccgtcgtagg gctcgaagcg cgcctgccga cgcaactggt cgatcgcctt
      901 gaaccgccct gccgacacga gccacgccct ggggttggcc ggtacgccgt cgcgcggcca
      961 gcgctcaagc gctgctcgga acgcttcgtg cagcgcctcc tccgcaaggt cgaagtcgcc
     1021 aagcagtcgg atcagcgtcg cgagcaccgc gcgcgattcg gtgcggtaga tcgcctcgac
     1081 ggtcggctgc gcgccgtcgt gcttcacgga cgacatcggg tcgccttgcc catcaggcgt
     1141 cgcgtcgcgc catcgggcgc gtgaccacga tgtcgacggt ctcggtgacg ggatcgaaca
     1201 ggatcctcgc ctcgccgcgc tcgagctgcc gcatcacctg cccgaccttg gtgtcgtgct
     1261 cgacgtcgcg ctcgccgtaa tcggtgcctt cgcgcagcac gaaggcctcg accacgccgc
     1321 gcagggccgc cgccgacagc tccgcatgcg gcacctcgat cggcggtggt cgatcgcgcg
     1381 ggtcggcgtc gtggcattcg tccaagcttc gctccgtcgt gaggtggtgc gcagaggtaa
     1441 ggtaggcttg ccggatgcac cacatcaagc gttcgatttc atcgcgaccg gcgcgcgtgc
     1501 agcgtacgac gtgcctcgtc gtcgcctgct ccctgctgct gccgggcgcg tacgccgcga
     1561 ccccgtcgtt tccggaagcc ccctatcgca agcacgtcga agtgctttct tcggacgcct
     1621 tccagggccg tgccccgggt accgagggcg agaagaagac ggtcgaatac atcgagcagc
     1681 agttcgccgc ggcgggtctc tccggcgcga tcgaaggcag tttccggcag gcggtgcccg
     1741 tgaccgagat cacgccgcac cccgacgagg cgctgcgggt gcgcggcagc gacggccgct
     1801 cgctcgatct cgcgacgctc gacgacgtca tcgtctggac ccggcgcccg gtgccggagt
     1861 cgcagatccg cgacgcgcag gtcgtctttg ccggttacgg catcgtggcg ccggaatatg
     1921 gctggaacga ttacgccggg ctcgacgtgc gcggcaagct cgtgctcgcg ctcgtgaacg
     1981 acccggggta tgcgacgcag gacccgaagc tcttcaccgg caacgcgatg acgtactacg
     2041 ggcgctggac ctacaagtac gaggaggccg tgcgccaggg cgcggccggg ctgctcgtcg
     2101 tgcacgagac caaagccgcg ggttacccgt gggacgtgcc gcgcagcggt gcgacccagg
     2161 cgcagttcga cctgcgcctg gcggacttcg agtcgcagcg cctgccgatc gagggctgga
     2221 tcaccgaggc cgcggcggcg cgcgtgctgt cgctcgctgg ccaggactat gccgcgctca
     2281 agaaggcttc ctccagccgc ggcttccgcg gcaaggccct ggacctgcgc gcgagcgtcg
     2341 gcgtgcgcaa cgacgtacag cacaagacct cgtacaacgt cgtcggcgtg ctgcgtgggc
     2401 ggacccgccc tgacgaggcg ttcgtctaca cggcgcactg ggaccacctt ggcgtcaagg
     2461 gcgacgcgaa gcccggcgag gacgcgatct acaacggcgc gcaggacaac gcgaccggca
     2521 tcgccgcgtt gatcgagctg gcgcgggcgt ttgcggccac cagaccacga ccgcaacgtt
     2581 cgctgctgtt cgttgccgtg accgccgagg agtcaggcct gctcggcagc gaatatttca
     2641 tcgcgcaccc gccggtgccc gcgtcgcgca tcgcgggcgg cctcaacatg gacaacctgt
     2701 acgcggtcgg cccgacgagg gacgtgacgg tgatcggctt cggcaagtcc gaactcgagg
     2761 acgagttgcg gcg
//
LOCUS       DASPFR010000365         5509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1818206, whole genome shotgun sequence.
ACCESSION   DASPFR010000365 DASPFR010000000
VERSION     DASPFR010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818206"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /gene="tilS"
                     /locus_tag="VD737_09670"
     CDS             complement(<1..788)
                     /gene="tilS"
                     /locus_tag="VD737_09670"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HXW10874.1"
                     /translation="MNAFTLDRFAQALEMALGPLPAGARPPRLCVALSGGLDSSVLLA
                     ALADLRQAGAASARAIRALHVDHSLHPDSARWAEASRVLAESLGVAYQPIRVDASAAR
                     GDSPEAAARAARYAAFLGHLAPDEVLLTAHHADDQLETVWLQWLRGGGLRAVAGMAPC
                     APLGAHAWHVRPLLGFERRELEQFGHARGLQWVEDPSNANLRFDRNYLRHVVLPTVYA
                     RWPAAVRTVGRVAGFAREALEIEDSLVEQDLPGVLRGRALDVASL"
     gene            complement(826..1788)
                     /locus_tag="VD737_09675"
     CDS             complement(826..1788)
                     /locus_tag="VD737_09675"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HXW10875.1"
                     /translation="MPLTFLEFEQPIAELEAKIDELRFVTSDAEVNIGEEIARLRAKS
                     RALTTSIFANLTPWQVAQLARHPQRPYTLDYASAVFDEFHELHGDRMYADDQAIVGGL
                     ARLAGKPVMLIGHQKGRDTKERVRRNYGMPKPEGYRKALRLMKLAERFRLPLITLIDT
                     PGAYPGIGSEERNQSEAIARNLFEMSTLRTPVITAVIGEGGSGGALAIGVCDRLIMLQ
                     YSVYSVISPEGCASILWKSADKKEIAAEAMGITAERIDKFGVVDEIIKEPLGGAHRDP
                     QAMADDLKAALLRHLEAVEALSDDELLARRYERLRNQGVYRTTP"
     gene            complement(1910..2410)
                     /locus_tag="VD737_09680"
     CDS             complement(1910..2410)
                     /locus_tag="VD737_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10876.1"
                     /translation="MSTPFRQWQQAVVMDIDAWLKELHVPREAYRIVSLLPLVYVAWA
                     DGKVQGAERNLVLSIARERGLLENGGAEALERWLTERPTPRQLKADIAVLNELARSDR
                     KFAAEFGADEEQLLLAWCQDVADAAGGLLGLRSARRDAELDALKTIAGALQVHGAKPW
                     RAVLSS"
     gene            complement(2510..>5509)
                     /gene="dnaE"
                     /locus_tag="VD737_09685"
     CDS             complement(2510..>5509)
                     /gene="dnaE"
                     /locus_tag="VD737_09685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HXW10877.1"
                     /translation="FLKPDDFEAHEARVCIHDGALLTDVSRPRRYSEQQYLRSPDEMA
                     QLFSDLPEALDNSVEIARRLNLEIRLGRPSLPAYPVPAGQTTEDHLREESRLGLERRW
                     PVLMQAPGAAISRDDYDRRLESELDVICPMGFAGYFLIVADFIRWARENGVPVGPGRG
                     SGAGSLVAYALGITDLDPLRYDLLFERFLNPERVSMPDFDIDFCMEGRDRVIDYVAHR
                     YGRDRVSQIITYGTMAAKAVVRDVARVQGLSYGFADSIAKLIPFELGITLDDALEKEE
                     ELRKRYRNEEEVREVIDMARSLEGLVRNAGMHAGGVVIAPSVLTDFAPLYCDENGTSV
                     VTQFDKDDVEAAGLVKFDFLGLRTLTIIDWAVRTINDARAAAGEAALDMATLPMDDAP
                     TYALLKRCETTAVFQLESRGMKDLIKRLQPDTFEDIVALVALFRPGPLQSGMVDDFID
                     RKHGRIGGGIDYLHPDLQPVLYPTYGVILYQEQVMQIAQVLAGYTLGGADLLRRAMGK
                     KKPEEMAKQRSIFVTGATARGVAEHTAAHIFDLMEKFAGYGFNKSHSAAYAVLSYQTA
                     WLKAHYPAAFMAAVLSSDMDKTDKVVTLIDECNRMGLAVEPPDINASQYMFAVSGPKS
                     IRYGLGAIKGVGHAAVENMIEERGTHGHFRDLRDLCRRLDLNKVNRRVLEALIRAGAC
                     DSFGGMRCRASLMHQLPAAMQAADQSTRAKQAGQTDLFGLSDPSSPATAAEVARRPGD
                     GSAAGAGSDLSRAAGEVARSAGEGSPEWSDAVRLAGERETLGLYLTGHPIAEYERELK
                     PIISGRIVDVGGARPVGAGEDKGGGWRGPGRNVTVAGLVLEIRKRGGRTSFVLDDRSG
                     RLEVAMFEDVYQQFRALVTKDAILVVEGGLRWDDFIDDWRLQARRILDVDQAREQYAR
                     NLVLRWPAGKHRNGDAGKFVTALEHALKPSKGGKCNVAVRYATGDAAAILQFGEQWKV
                     RPSRELIERLGTLVGRDSVELYYAPRE"
BASE COUNT          799 a         1926 c         1913 g          871 t
ORIGIN      
        1 aggctcgcga cgtccagtgc gcgcccgcga agcacgcccg gcaggtcctg ctcgacgagc
       61 gagtcctcga tctcgagggc ctcgcgcgca aaaccggcga cgcgaccaac ggtgcggacg
      121 gcggccggcc acctcgcgta caccgtcggc agcacgacgt ggcgcaggta gttgcgatcg
      181 aagcgcaggt tcgcgttcga cggatcctcg acccattgca ggccgcgggc gtgcccgaac
      241 tgctcgagtt cccgtcgttc gaaaccgagc agcgggcgca cgtgccaggc gtgcgcgccg
      301 agcggagcgc acggtgccat gccggccacg gcgcgcagcc cgccgccgcg cagccactgc
      361 agccagaccg tctcgagttg gtcgtcggcg tggtgggcgg tgagcagcac ctcgtccggt
      421 gccaggtgcc cgaggaaagc cgcgtagcgc gccgcccgtg ccgccgcctc cgggctgtcg
      481 ccccgcgcag cgctcgcgtc gacgcggatc ggctggtacg cgacaccgag cgactccgcg
      541 agcacccggc tcgcctcggc ccagcgcgcc gagtcgggat gcaacgagtg atcgacgtgc
      601 agcgcgcgta tcgcgcgtgc ggacgcggca cccgcctgcc gcaggtccgc cagtgccgcg
      661 agcaggacgc tcgagtcgag tcccccgctc agggccacgc acagccgcgg cgggcgcgcg
      721 ccagcaggca acggccccag cgccatctcg agggcctgcg cgaagcgatc gagcgtgaag
      781 gcgttcatgg ccggcgcagc gcgagggcgg ctcgggacgc cgcgctcagg gcgtggtgcg
      841 atagacgccc tggttgcgca gccgctcgta gcgccgggcg agcagctcgt cgtcggacaa
      901 cgcctcgacc gcctcgaggt gccgcagcag cgccgccttc aggtcgtcgg ccatcgcctg
      961 cgggtcgcgg tgggcgccac cgagcggctc cttgatgatc tcgtcgacca cgccgaactt
     1021 gtcgatgcgc tccgcggtga tgcccatggc ttccgcggcg atttccttct tgtcggcgct
     1081 cttccagagg atcgaggcgc agccttcggg cgagatcacc gagtacaccg agtactgcag
     1141 catgatcagc cggtcgcaga ccccgatcgc gagcgcgccg ccggaaccgc cctcgccgat
     1201 cacggccgtg atcaccggcg tgcgcagcgt cgacatttcg aacaggttgc gcgcgatggc
     1261 ctcgctctgg ttgcgctcct cggaaccgat gccagggtag gcgcccggcg tgtcgatcag
     1321 cgtgatcagc ggcagccgga agcgctcggc gagcttcatg agccgcagtg ccttgcgata
     1381 gccctccggc ttgggcatgc cgtagttgcg gcgcacgcgc tccttggtgt cacgtccctt
     1441 ctggtggccg atcagcatga cgggcttgcc ggcgagccgg gcgagcccgc cgacgatcgc
     1501 ctggtcgtcg gcgtacatgc ggtcgccgtg cagttcgtgg aactcgtcga acaccgcgct
     1561 tgcgtagtcg agcgtgtacg ggcgctgcgg gtggcgcgcg agctgcgcga cctgccacgg
     1621 cgtcaggttg gcgaaaatgc tggtcgtgag cgcgcggctc ttcgcgcgca ggcgcgcgat
     1681 ctcctcgccg atgttgacct cggcgtccga agtcacgaag cgaagttcgt cgatcttggc
     1741 ctcgagctcc gcgatgggct gctcgaactc cagaaaagtc aatggcatgc gcgaaggatg
     1801 ccagtagcca gtagccagtg gctagtggtc agtggccagt gggattccgc gcgcgggtcg
     1861 cccgtggggc cggcgaatgc gatatccccg atgcagggac gaagccgggt caggacgaca
     1921 acacggcgcg ccacggcttc gcgccatgga cctggagcgc acccgcaatc gtcttgagcg
     1981 cgtcgagttc ggcatcgcgc cgcgccgagc gcagcccgag cagtccaccc gcggcgtccg
     2041 cgacgtcctg gcaccacgcg agcagcagct gctcctcgtc tgcgccgaac tcggcggcga
     2101 acttccggtc gctgcgcgcg agttcgttca gcaccgcgat gtcagccttc aactgtcgtg
     2161 gggtcggccg ttcggtcaac cagcgctcga gcgcctctgc gccaccgttc tcgagcaggc
     2221 cgcgctcacg cgcgatgctg aggacgaggt tgcgctcggc gccctggacc ttgccgtccg
     2281 cccatgcgac gtagacgagg ggcaggagcg agacgatgcg gtacgcctcg cgcgggacgt
     2341 ggagttcctt gagccacgca tcgatgtcca tgacgactgc ctgctgccac tgccggaacg
     2401 gcgtggacaa gttcttgctt gcctcgcttt atatacggtc cgcggatgga cgcaagagct
     2461 ctcgtagcag gggtcgccgg cgccgatcac cgtgcgaagg gcgcgaggct cactcgcgcg
     2521 gcgcgtaata cagctcgacc gaatcccgcc cgaccaatgt cccgagccgc tcgatcagct
     2581 cgcgcgacgg tcgcactttc cactgctcgc cgaactgcag gatcgcggcg gcgtcgccgg
     2641 tcgcgtaccg caccgcgacg ttgcacttgc cgcccttgct gggcttgagc gcgtgctcga
     2701 gcgcggtcac gaacttgccg gcatcgccgt tgcggtgctt gccggccggc cagcgcagca
     2761 cgaggttgcg cgcgtactgt tcgcgggcct ggtcgacgtc caggatccgg cgcgcctgca
     2821 ggcgccagtc gtcgatgaag tcgtcccagc gcaggccgcc ctcgacgacg aggatcgcgt
     2881 ccttggtcac gagcgcgcga aattgctggt acacgtcctc gaacatcgcc acttcgaggc
     2941 ggccgctgcg atcgtccagc acgaagctcg tgcggccgcc gcgcttgcgg atctcgagca
     3001 ccagtcccgc gacggtcacg ttgcggcccg ggccacgcca gccaccgccc ttgtcctcgc
     3061 ccgcgcccac cggcctggcg ccgccgacgt cgacgatgcg cccgctgatg atgggcttca
     3121 gttcgcgttc gtactccgcg atcgggtgcc cggtgaggta gagcccgagt gtctcgcgtt
     3181 cgccggcgag ccgcacggcg tcgctccatt cggggctgcc ctcacccgcg ctgcgcgcga
     3241 cctctcccgc agcgcgggag aggtcggagc ccgcacccgc tgcgctgccg tcacccggtc
     3301 ggcgcgcgac ctctgccgcg gtggccggag agctgggatc ggagaggccg aacaggtccg
     3361 tctggcccgc ctgcttcgcg cgggtcgact ggtccgccgc ctgcatcgcg gccggcaact
     3421 ggtgcatcag cgacgcacgg cagcgcatgc cgccgaagct gtcgcaggca cccgcgcgga
     3481 tcagcgcctc gagcacgcgg cggttgacct tgttgaggtc gaggcggcgg cacaggtcac
     3541 gcaggtcgcg gaagtggccg tgggtgccgc gctcctcgat catgttctcg accgcggcgt
     3601 gaccgacgcc cttgatcgcg cccagcccgt agcggatcga cttcgggccc gacaccgcga
     3661 acatgtactg cgacgcgttg atgtcgggcg gctcgaccgc gaggcccatg cggttgcact
     3721 cgtcgatcaa cgtcacgacc ttgtcggtct tgtccatgtc cgacgacagc acggccgcca
     3781 tgaaggcggc cgggtaatgc gccttgagcc acgccgtctg gtacgacagc acggcgtagg
     3841 cggcggagtg cgacttgttg aagccgtacc ccgcgaactt ctccatcagg tcgaagatgt
     3901 gcgcggccgt gtgctcggcc acgccacgcg cggtcgcgcc cgtgacgaag atcgagcgct
     3961 gcttcgccat ctcctcgggc ttcttcttgc ccatcgcgcg gcgcaggagg tcggcgccgc
     4021 cgagcgtgta gcccgcgagc acctgggcga tctgcatgac ctgttcctgg tacaggatca
     4081 cgccgtacgt ggggtagagc accggctgca ggtccgggtg caggtagtcg atcccgccgc
     4141 cgatccggcc gtgcttgcgg tcgatgaagt cgtcgaccat gccggactgc agcggacccg
     4201 ggcggaacag cgccacgagc gcgacgatgt cctcgaaggt gtcgggttgc aggcgcttga
     4261 tcaggtcctt catgccgcgc gattcgagct ggaacaccgc ggtcgtctcg cagcgcttca
     4321 gcagcgcgta ggtcggcgcg tcgtccatcg gcagcgtcgc catgtcgagc gcggcctcgc
     4381 cggccgccgc gcgcgcgtca ttgatcgtgc gcacggccca gtcgatgatc gtgagcgtgc
     4441 gcagcccgag gaagtcgaac ttgaccaggc ccgcggcctc gacgtcgtcc ttgtcgaact
     4501 gcgtgacgac actcgtgccg ttctcgtcgc agtagagcgg cgcgaagtcg gtgagcaccg
     4561 acggcgcgat cacgacgccg cccgcgtgca tgcccgcatt gcgcacgagg ccctcgagcg
     4621 agcgggccat gtcgatgacc tcgcgcacct cttcctcgtt gcgataacgc ttgcggagtt
     4681 cctcctcctt ctcgagcgcg tcgtcgagcg tgatgccgag ctcgaacggg atcagcttgg
     4741 cgatgctgtc cgcgaagccg tagctcaggc cctgtacgcg cgcgacgtcg cgcacgaccg
     4801 ccttcgcggc catggtgccg taggtgatga tctgcgagac gcggtcgcgc ccgtagcggt
     4861 gcgcgacgta gtcgatcacg cggtcgcggc cttccatgca gaagtcgatg tcgaagtccg
     4921 gcatcgacac gcgttcggga ttcaggaacc gctcgaacag caggtcgtag cgcagcgggt
     4981 cgaggtcggt gatgccgagc gcgtatgcga ccagcgaccc cgcgcccgag ccgcggcccg
     5041 ggccgaccgg tacgccgttc tctcgcgccc agcgtatgaa gtcggcaacg atcaggaagt
     5101 agcccgcgaa ccccatcggg cagatcacgt cgagctcgct ttcgagccgt cgatcgtagt
     5161 cgtcgcgcga gatggcggcg cccggggcct gcatcagcac tggccagcgc cgctcgagcc
     5221 cgagcctcga ttcctcccgc aggtggtcct cggtcgtctg tccggcgggc accgggtatg
     5281 caggcagcga cggcctgccg agccggatct cgaggttgag ccgccgcgca atctcgaccg
     5341 agttgtcgag cgcctcgggc aggtccgaga acagctgcgc catctcgtcg ggcgagcgca
     5401 ggtactgctg ctcgctgtaa cgccgtggtc gcgacacgtc ggtgagcagg gcgccgtcgt
     5461 gtatgcacac gcgcgcctcg tgcgcctcga aatcgtccgg cttcaggaa
//
LOCUS       DASPFR010000366         2575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_354820, whole genome shotgun sequence.
ACCESSION   DASPFR010000366 DASPFR010000000
VERSION     DASPFR010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354820"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VD737_09690"
     CDS             <1..608
                     /locus_tag="VD737_09690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HXW10878.1"
                     /translation="QRLERYLSFLTKWNNKINLTAFSLVEPSDEALDRLLVEPVIAAA
                     HIPDASRRMIDIGSGSGSPAIPLLLAAPRLGGTMVESKTRKGVFLLEALRHLEVSNWT
                     VETARFEQLLTRADLHEVFDGLTIRAVRMEKPVLLTLQAFVRPGGALYLFQATQRRLA
                     LDHSSFPLLEDRTVPLVESLSSSLTILRKDVPRGTRSAEAS"
     gene            856..1629
                     /locus_tag="VD737_09695"
     CDS             856..1629
                     /locus_tag="VD737_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HXW10879.1"
                     /translation="MARIVTITNQKGGVGKTTTAINLAASLAMADQRVLVIDLDPQGN
                     MTSGVGLRGVRGAGGSIYEALTSDVAIDPDSLILPTGVDNLFAIPADRQLTGAEIELV
                     GLPQREFRLRHFVEPLRARFDRIFIDCPPSLGLLTLNALVAADAVLIPLHCEYFALEG
                     LADLVATLKRVRASLNPALDIDGVLLTMHDERTNLGQQVARDVREFFQEKVFRTVIPR
                     NVRLGEAPSHGKPVVLYDARSRGAEAYLSLAREVIARAS"
     gene            1626..2573
                     /locus_tag="VD737_09700"
     CDS             1626..2573
                     /locus_tag="VD737_09700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HXW10880.1"
                     /translation="MTARTPPRSDSIETGNQGRSTMEKRPALGKGLSALIPDTPLGPP
                     QPGPLPGVLELEVDRLTPNDYQPRLNIDDARLEQLAASIRANGVIQPIVVRKVDDGYR
                     IIAGERRWRAARLAGLTRVPVVVKEVSGDATEQRVLQMALVENIQRDDLNPIDEAKAY
                     RRLADEFTLTQEAIASAVGKDRATVANTLRLLKLPGDVQELVASGELSMGHARALLAL
                     AGEQSQRQVAREVKARGLSVRETEALVKRELEGASRSRTSRPAAPAADVHTRAAEDKL
                     KLQLGTRVRIKRKGKGGRIEIDFTSEDELIRIYEALTER"
BASE COUNT          466 a          871 c          816 g          422 t
ORIGIN      
        1 tccaacggct cgaacgctat ctgagttttc tcacgaagtg gaacaacaag atcaacctga
       61 cggcgttcag tctcgtcgaa ccgagcgatg aagcgcttga ccggctgctg gtcgagccgg
      121 tcatcgcggc tgcgcacata ccggacgcgt cacggcgaat gatcgacatc gggtccggca
      181 gcgggtctcc agccatacca ttgctgctcg cagctccacg gctcggcggc acgatggtcg
      241 aatcaaagac ccgcaagggc gtcttcctcc ttgaagctct caggcatctc gaggtctcga
      301 actggaccgt tgagaccgca cgcttcgagc aactactcac acgggccgac ctccacgaag
      361 tcttcgacgg gctgacgatc cgggctgtgc gcatggagaa gccggtgctg ctgacactcc
      421 aggcgttcgt ccggcccggc ggcgcgctct acctctttca agccacccag cgtcgactcg
      481 cgctcgatca ctcgtccttt ccgttgctgg aggaccgaac tgtgcccctc gtcgagtcgc
      541 tatcaagttc gctcacgatc ctccgcaaag acgttccacg tggaacacgg tccgccgagg
      601 ccagctagtg gcctgtcgcc gctgaacagt atcgcctgga gcccatggag tccgcggcgc
      661 cggcccgcaa gacgcgagga tcgcaaatat tgggaatctg cggcaattgg caatgcttct
      721 gcggagacag accacagatt gttccacgtg gaacactctc gttgttgact ccccgcagac
      781 gattctccta tgcttgactg tcgttctcgg ttgtcacaag tgccataaat agctgtaaac
      841 acaacactta cctcaatggc acgaattgta accattacaa accagaaggg cggtgtcggc
      901 aagacgacca cggccatcaa cctcgcggcg tcgctcgcca tggccgatca gcgcgtgctg
      961 gtgatcgacc tcgacccgca gggcaacatg accagcggcg tcggcctgcg cggcgttcgt
     1021 ggcgccggag gctcgattta cgaagccctg acctcggacg tcgccatcga tcccgattcg
     1081 ctcatcctcc cgacgggcgt cgataacctg ttcgccattc cggcggatcg gcaactgacc
     1141 ggcgcggaga tcgagctcgt cggtctgcca caacgtgagt tccggctccg ccatttcgtc
     1201 gagcccctgc gcgcccgctt cgatcgcatc ttcatcgact gtcctcccag cctcgggctg
     1261 ctgaccctca acgcgctcgt ggccgccgac gccgtgctga ttccgctgca ctgcgagtac
     1321 ttcgcgctcg agggcctggc tgacctggtc gcgacgctga agcgcgtccg tgcgtcgctc
     1381 aacccggcgc tcgacatcga cggcgtgctc ctgacaatgc acgacgagcg caccaatctc
     1441 ggccagcagg tcgcacgcga cgtgcgggag ttctttcagg aaaaggtgtt tcgcacggtg
     1501 attccccgca acgtgcggct cggcgaggcg cccagccacg gcaagcccgt ggtgctgtac
     1561 gacgcgcgct cgcgcggcgc cgaagcctac ctgtcgctcg cacgggaagt catcgcgcgc
     1621 gcgtcgtgac cgcgcggacg ccgccgcgga gtgattccat cgagactggc aaccaaggac
     1681 gttcaaccat ggagaaacgc cccgcgctcg ggaaagggct gagcgcgctc attcccgata
     1741 cgccgctggg cccgccgcag ccggggccgc ttccgggggt tctcgagctg gaggtcgatc
     1801 gactcacgcc gaacgactac cagccgcgcc tcaacatcga cgacgcgcgc ctggagcaac
     1861 tggcggcgtc gattcgggcc aacggcgtga tccagccgat cgtcgtgcgc aaggtcgacg
     1921 acggctaccg gatcatcgcc ggcgagcgcc gctggcgtgc cgccaggctc gccgggctga
     1981 cccgcgtccc cgtggtcgtc aaggaggtgt cgggcgatgc caccgagcag cgggtcctgc
     2041 agatggccct ggtcgagaac atccagcgcg acgacctgaa cccgatagac gaggccaagg
     2101 cctatcgtcg tctggccgac gagttcaccc tgacccagga ggcgatcgcg tccgccgtcg
     2161 ggaaggatcg cgccacggtc gccaacacgc tgaggctgct gaagctgcct ggcgacgtcc
     2221 aggagctcgt cgcctcgggc gagctgagca tggggcacgc gcgcgcgctg ctcgcgctgg
     2281 ctggggagca gagccagcgg caggtcgcgc gcgaggtgaa ggcgcgcggg ctgtcggtgc
     2341 gcgaaaccga ggcgctcgtg aagcgcgagc tcgaaggcgc gtcgcgaagc aggacatcgc
     2401 ggcctgccgc cccggccgcc gacgtccaca cgcgcgcggc cgaggacaag ctgaagctgc
     2461 agctcggcac gcgcgtgcgc atcaagcgca agggcaaggg cggccggatc gagatcgact
     2521 tcaccagcga agacgagttg atccgcatct acgaggcatt gacagagcgc taaag
//
LOCUS       DASPFR010000367         5450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_719181, whole genome shotgun sequence.
ACCESSION   DASPFR010000367 DASPFR010000000
VERSION     DASPFR010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719181"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..610
                     /locus_tag="VD737_09705"
     CDS             62..610
                     /locus_tag="VD737_09705"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HXW10881.1"
                     /translation="MTAKRSVRCAASLSCLVALASGCAPFQPGLPSEPVAREARSAAG
                     RGDTLAREARAQLGVPYRWGGGDPVRGFDCSGLVAWVHSREGIAVPRTAAAQFAAAER
                     VDERDLRPGDLVFFRLQSRRGDVSHVGIYTGQRRFVHAPQTGRDVAEANLDDPYYRAR
                     YAGAGRFYEEPGGRGPRFGSPR"
     gene            637..846
                     /locus_tag="VD737_09710"
     CDS             637..846
                     /locus_tag="VD737_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10882.1"
                     /translation="MPRKYDCPCCDGQGLVHADSTDAEPLECVECDSTGFVTREHREA
                     LLAWRHKCRLRRPSGESVEAGAQDG"
     gene            complement(871..1785)
                     /locus_tag="VD737_09715"
     CDS             complement(871..1785)
                     /locus_tag="VD737_09715"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HXW10883.1"
                     /translation="MERLVAPDAAAAANDAGGSASAVGSCCDYADRLREAADLLQAQR
                     ANPYRVSAYRKAADAISTLDHAAIVDLVEREGIDGLDRLPHVGRGIATAIVEMVRTGH
                     WSQLERLRGTCDPVQLFTVVPGLGHRLAERIYEELHVDTLEGLELAAHDGRLENVPGV
                     GPRRAAAIRASLHAMLVRDRGRNAPAVGEGPRPSTRLLLAIDRTYREQAAADALPRIA
                     PIRFNPMQEAWLPVLHCERDGWQFTAMFSNTAQAHQLNRTHDWVRVFFYDSENSEGQH
                     TVVTETHGPLAGRRVVRGRESECRSIYA"
     gene            1919..3415
                     /locus_tag="VD737_09720"
     CDS             1919..3415
                     /locus_tag="VD737_09720"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXW10884.1"
                     /translation="MSLGTALRRLRHALRSLLAALLLVAVGFYLHYLWAGPELEPWHR
                     AVLDAEFTAADYREGRVTTLAQYVAREHELQRQLDDEIRSRVPLQEQRPFERYAAGSR
                     SDPAVWTTNWNLTYVLEPAVAPRGAVLLLHGLTDSPYSLRSLALALVADGRRVVGLRL
                     PGHGTAPAGLLRFEPEDLRAATRLAMLDLRRSLGPGLPIDVIGYSNGAALAVDYALDA
                     REDAALPRPSRLVLVSPAIGITRLAALGRIRTGLSELQGFGRAAWQTVEPEIDPFKYQ
                     SFPWHAAGVTQRLTSSLTRRVARLAERGPVSGLPPILAFVSTVDSTVQAGKVADALLD
                     KLAAEGHELVLFDVNRWSLVQTLLVEDPGPLTRRLLERPRRPFALTLVTNVSAATPLV
                     EERRVPAFATEARRHPLGVEWPRGVFSLSHVALPFPPDDPLYGYDAPRAVQHVQLGRF
                     EARGENGVLEVPNWLLTRQRSNPFHGYLVARVRGWLDAPVRQTEDTSR"
     gene            3412..4974
                     /locus_tag="VD737_09725"
     CDS             3412..4974
                     /locus_tag="VD737_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HXW10885.1"
                     /translation="MTRWLRTLVLVAVLPLLAHCASAPRMDGIVATYAVPPATHAPLD
                     QAVLRDLGRDPALSTVRLLENNALAFAYRAASAAAAERTLDVQYYIWHEDLTGRLLAA
                     ELLRAAERGVRVRVLIDDLDARDKHESLAVTDLHPNIEVRLFNPFYSRAGLWGWASEW
                     VLRASRLNHRMHNKSWIADNRIAIIGGRNIGDEYFGASPHSNFADLDVALTGAVVAEV
                     SGQFDDYWNSTNAVPIDRFDVRKPKQKDLEALILRARQYREDVADTPYVRALRDVQSR
                     TQLLADAPEALHVTDVRLLVDDPSKVGGKRGIDGSKVLAGLERAMATARREVLIISPY
                     FVPGQEGAARLVRDVEQGLRVAVLTNSLAATDVAAVHVGYARVRRQLARGGVELYEMK
                     RKAASPDGRSQLSVTGSSGASLHSKAVLIDDRWVYVGSMNLDPRSAILNTEMGVLVES
                     PELAAQLRAQWDLITSPELSYRVVLEPDGELVWYDRVGGRERRSQHEPDASTLRRIGV
                     TLLRLVPIDSQL"
     gene            4997..>5450
                     /locus_tag="VD737_09730"
     CDS             4997..>5450
                     /locus_tag="VD737_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10886.1"
                     /translation="MLQTPTTASAPPPLQRRGRGIRTWVWALLAVAVVLGALYTSLML
                     WYSYSEGERAGVLQKFSHRGWICKTYEGELAQYVVGGVAPQIWHFSARDAAVVTQLRE
                     AVGRQVRIHYEEHRGLPTNCFGETAYFAKSVEVIGPPPAAAAPATGAGP"
BASE COUNT          780 a         1916 c         1941 g          813 t
ORIGIN      
        1 ccgcgcggcg cggtcacaca gggcaatgag gcgagcccac atcggaccac acgcgatgca
       61 ggtgaccgcc aagaggtccg ttcgttgtgc ggcgtcgctc agctgtctcg tggcgctggc
      121 gagcggctgc gcgccattcc agcccggctt gccctccgag cccgtcgcgc gcgaggcacg
      181 cagcgccgcc ggccgcggcg acacgctggc gcgcgaggca cgggcccagc tcggcgtgcc
      241 ttatcgatgg ggcggcggcg atcccgtgcg cggtttcgac tgcagcggac tcgtcgcgtg
      301 ggtgcactcg cgcgaaggga tcgccgtgcc gcgcacggcg gcagcccagt tcgcggcggc
      361 cgagcgcgtc gacgagcgcg acctgcgccc gggcgacctg gtctttttcc ggctgcagtc
      421 gcgacgcggc gacgtgagcc acgtcggcat ctacaccggc cagcgcaggt tcgtgcacgc
      481 gccacagacc ggacgcgacg tcgccgaggc gaatctcgac gatccctact atcgcgcgcg
      541 atacgcgggc gccggccggt tctacgagga gccgggcggg cgggggccgc ggttcggatc
      601 gccgcgctga gctctgccgt tcacatggag gtgaccgtgc ccaggaaata cgactgcccc
      661 tgttgcgatg gccagggcct cgtgcacgcc gactcgaccg acgccgagcc gctcgagtgc
      721 gtcgagtgcg attccaccgg gttcgtcacg cgcgagcacc gcgaggcgct gctcgcgtgg
      781 cgacacaagt gccggttgcg acgccccagc ggtgaatccg tggaggcggg tgcgcaggac
      841 ggctgagacc gtgtccgtgg cgtcgtcgcc tcaggcgtag atcgaccggc actcggactc
      901 gcggccgcgc acgacgcggc gtcccgcgag cggcccgtgc gtctcggtga ccacggtgtg
      961 ctgaccctcg gagttctccg agtcgtagaa gaagacccgc acccagtcgt gcgtgcggtt
     1021 gagctggtgc gcctgcgccg tgttggagaa catcgccgtg aactgccagc cgtcgcgctc
     1081 gcagtgcagg accggcagcc acgcctcctg catcggattg aagcggatcg gggcgatcct
     1141 cggcagcgcg tcggccgcgg cctgctcgcg atacgtacgg tcgatcgcca gcagcagccg
     1201 cgtcgacgga cgggggccct cgccgacggc cggagcgttg cgaccgcggt cgcgcacgag
     1261 catcgcatgc aggctcgcgc gtatggcggc ggcgcgtcgc ggccccacgc caggcacgtt
     1321 ctcgaggcgt ccgtcgtgcg cagcgagttc cagtccctcg agcgtgtcga cgtgcagttc
     1381 ttcgtagatg cgctccgcga ggcgatggcc gagtccggga acgacggtga acaactggac
     1441 tggatcgcag gtgccgcgca ggcgctcgag ctggctccag tggcccgtgc gcaccatctc
     1501 gacgattgcc gtcgcgatgc cgcggccgac gtgcggcagg cggtcgaggc cgtcgatgcc
     1561 ttcgcgctcg acgaggtcga cgatggccgc gtggtccagg gtcgaaatcg cgtcggccgc
     1621 cttgcgatac gccgagacgc ggtacgggtt cgcgcgttgg gcctgcagca ggtccgccgc
     1681 ctcgcgcagc cggtctgcat aatcgcagca cgacccgacc gcggatgcgg agccgccagc
     1741 gtcgtttgcc gccgccgccg catcgggtgc caccagtcgt tccatcttgt cgctcgtcgc
     1801 gtgttgcgac ctgccatcgg caatgctgac gaggattgcc acgagggacc attcgggttt
     1861 tacgagacag ggccgattca gacatgatcc agatcgggca gcgaagagcg ggtcgcggat
     1921 gagcttggga acggccttgc gtcggcttcg gcatgcgctg cgctcgctgc tcgcggccct
     1981 gctgctcgtc gccgtcggct tctacctgca ctacctctgg gccggaccgg agctcgagcc
     2041 ctggcatcgc gcggtgctcg atgccgagtt caccgcggcc gactatcgcg agggccgagt
     2101 caccacgctc gcacagtacg tcgcgcgcga acacgagctg cagcgacagc tggacgacga
     2161 gatccgttcg cgcgtgcccc tgcaggagca gcgtccgttc gagcggtacg ccgccggcag
     2221 tcgcagcgac ccggccgtgt ggacgaccaa ctggaacctg acctacgtgc tcgaacccgc
     2281 ggtggcgccc cgcggtgcag tgctgctgct gcacggcctc accgattcgc cgtacagcct
     2341 gcgcagcctc gcgctcgcgc tcgtggccga cggacgcagg gtcgtcggtc tgcggctgcc
     2401 gggtcacggc accgcgccgg cgggcctgtt gcggttcgaa cccgaagacc tgcgagcggc
     2461 cacgcgtctc gcgatgctcg acctgcgtcg gtcgcttggt cccggcctgc cgatcgacgt
     2521 catcggctac tcgaacggcg cggcgctcgc ggtcgattac gcgctggacg cacgcgagga
     2581 tgccgcgctg cctcgaccgt cgcgactcgt gctcgtgtcg ccggccatcg gcatcacgcg
     2641 gcttgccgcg ctcggccgca tccgcaccgg gctctccgag ctgcagggct tcggccgcgc
     2701 cgcgtggcag accgtcgagc cggaaatcga tccgttcaag taccagtcgt ttccgtggca
     2761 cgcggccggc gtgacgcaac ggctgacgag ctcgctgacg cgccgcgtcg cgcgactcgc
     2821 tgagcgcgga cccgtgagcg gcttgccgcc gatcctcgca ttcgtgtcga cggtggactc
     2881 gaccgtgcag gcgggcaagg tcgccgatgc gctgctggac aagctcgccg cggaagggca
     2941 cgagctcgtg ctgttcgacg tcaaccgctg gtcgctcgtg cagacgctgc tggtcgagga
     3001 tcccgggccc ctcacgcgcc gcctgctcga gcggccacgc cggcctttcg cgctgacgct
     3061 cgtcaccaac gtatccgcgg cgaccccgct cgtcgaggaa cggcgagtgc cggcgttcgc
     3121 gaccgaggcc cggcgccacc cactcggcgt cgaatggccg cgcggcgtct tctcgctgtc
     3181 gcacgtcgcg ctgccgttcc cgccggacga tccgctgtac ggctacgacg cgccgcgcgc
     3241 cgtgcagcac gtgcagctcg gccggttcga ggcacgcggc gagaacggcg tcctcgaagt
     3301 gccgaactgg ctgttgaccc gccagcgcag caatccgttc cacggctatc tcgtcgcgcg
     3361 cgtgcggggg tggctcgatg cgccggtgcg ccagacggag gacacctcgc gatgacgcgc
     3421 tggctgcgca cgctcgtgct cgtcgcagtg ctgccgctgc tcgcgcattg cgccagtgcg
     3481 ccgcgcatgg acggcatcgt cgccacctat gcggtgccgc ccgccacgca cgcgccactc
     3541 gaccaggcgg tgctgcgcga cctcggccgg gatccggcgc tatccaccgt ccgcctgctc
     3601 gagaacaacg cgctggcctt cgcgtaccgc gccgcgtcgg cggcagcggc cgagcgcacg
     3661 ctcgacgtgc agtactacat ctggcacgaa gacctcaccg ggcggctgct ggcggccgag
     3721 ctgctgcgtg cggccgagcg cggcgtccgc gtgcgcgtgc tgatcgacga cctggacgcg
     3781 cgcgacaagc acgagagcct cgcggtcacg gacctccacc cgaacatcga ggtgcggctg
     3841 ttcaatccct tctacagccg cgcagggctg tggggctggg cgagcgagtg ggtgctccgt
     3901 gcgagccggc tgaaccatcg catgcacaac aagtcctgga tcgccgacaa ccggatcgcg
     3961 atcatcggcg gccgcaacat cggcgacgag tatttcggcg cgtcgccgca ctcgaacttc
     4021 gcggacctcg acgtcgccct gacgggggcc gtcgtggccg aggtgagcgg ccagttcgac
     4081 gattactgga acagcaccaa cgccgtgccg atcgaccggt tcgacgtccg caagccgaag
     4141 cagaaggacc tcgaggcact gatcctgcga gcgcgacagt accgcgagga cgtcgcggac
     4201 acaccctacg tgcgcgcgct gcgggacgtg cagagccgca cgcagctgct ggccgacgct
     4261 cccgaggcgc tgcacgtgac cgacgtgcgc ctgctggtgg acgacccgag caaggtcggc
     4321 ggcaagcgcg gcatcgacgg atcgaaggtg ctggcgggcc tcgaacgcgc catggccacg
     4381 gcgcgtcgcg aggtcctgat catctcgcca tacttcgtgc ccggccagga gggcgcggcg
     4441 cggctggtgc gagacgtcga gcaggggttg cgcgtggcgg tgctgacgaa ttcgctggcg
     4501 gcgaccgacg tcgcggccgt gcacgtcggc tatgcgcggg tgcgccggca gctcgcgcgc
     4561 ggcggcgtcg agctctatga gatgaaacgc aaggccgcca gcccggacgg gcgcagccag
     4621 ctcagcgtga cgggctcgtc cggtgccagc ctgcacagca aggccgtgct gatcgacgac
     4681 cgctgggtct acgtcgggtc gatgaacctc gatccgcggt ccgcgatcct gaacacggag
     4741 atgggcgtgc tcgtcgagag tcccgagctc gcggcccagt tgcgagcgca gtgggacctg
     4801 atcacgtccc ccgaactcag ttaccgcgtc gtgctcgagc cggacggcga gctcgtttgg
     4861 tacgatcgcg tcgggggacg ggaacgccgg tcgcagcacg aacccgacgc gagcacgttg
     4921 cgccggatcg gcgtgacgct gctgcgcctc gtgccgatcg attcgcagct ctgacaccga
     4981 ccacgagaac aacgcgatgc tgcagacacc cacgaccgcc agcgcgccgc cgcccctgca
     5041 gcggcgcggg cggggcatca ggacatgggt ctgggcgttg ctcgcggtcg ccgtcgtcct
     5101 cggtgcgctc tacacgtccc tgatgctctg gtattcgtac tcggagggcg agcgcgccgg
     5161 cgtgctgcag aaattctcgc atcgcgggtg gatctgcaag acctacgagg gcgagctcgc
     5221 acagtacgtg gtcggcggcg tggcgccgca gatctggcac ttcagcgcga gggatgcggc
     5281 agtcgtgacg caacttcgcg aggccgtcgg tcgccaggtg cgcatccact acgaagaaca
     5341 ccgtggcctg ccgaccaact gcttcggcga gaccgcctat ttcgcgaaga gcgtcgaggt
     5401 gatcggaccg ccgccggccg ccgccgcacc ggcgaccggc gccgggccga
//
LOCUS       DASPFR010000368         8787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1451877, whole genome shotgun sequence.
ACCESSION   DASPFR010000368 DASPFR010000000
VERSION     DASPFR010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8787
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451877"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VD737_09735"
     CDS             complement(<1..509)
                     /locus_tag="VD737_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10887.1"
                     /translation="MVSVSRLARACTIPLLALLGATAPAQDANVTRPVDELERLLSAE
                     PLVIAHAEISRPKAKGDITLKADVAFGGAEPLRVKLRKAEPGAEDFNNVPRYDVAAYE
                     LQKLFLDPAEYVVPPTALRFVSRPDFAKYSPDVAPTFPPADQVLAVVQYWLNDIKVIA
                     DVYDPARFAT"
     gene            complement(559..2037)
                     /locus_tag="VD737_09740"
     CDS             complement(559..2037)
                     /locus_tag="VD737_09740"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXW10888.1"
                     /translation="MLLLVVAFSFQGTRGIWEPDEGRYTSAAVNMHESGDWLVPTLDG
                     EHAHLTKPPVTYWAIAASFAALGHNEWAARLPSALAYVATGLLVLGLGRRFCRSRPWL
                     PAVVWGTSLAPGIAANIVSTDPILALFETAAMFAFVEAWSRDSALEARPWYRLMWLGW
                     GLAFMTKGPPALLPLLGVVLFLAMHDRPRLRPLFAPGGLLLFAVVAFTWFGVIVAGDP
                     DRLGYFLGYEVYDRVFTSAHDRNSEWYGGFEVYVPMLLGGTLPWSVLAVVAAGGPAAA
                     WRSLRAHWRARRRDDLLLAYWLLVPLVVFFLARSRLHLYVLPLFVPLALVMARTIQSW
                     PRATDRRLAVIAAAGVLALVALKALLGYWPHDRDARAFAAQLERLVDPHAIEEVVFVG
                     MRPFYGLNVYVDRHIEGIDIGRTPARGEYSKFVDHEDVCGELAEHERSVYGMKHKHTA
                     AFLAAAQSCGGRPQYLGKVYADGFDIVFYLVPPTAPDDGVSS"
     gene            complement(2085..2285)
                     /locus_tag="VD737_09745"
     CDS             complement(2085..2285)
                     /locus_tag="VD737_09745"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HXW10889.1"
                     /translation="MQRLLLLVGGLLVLAGLLWPWLSRLPFGRLPGDVVIDRPGFRFF
                     APFTTMIVLSIVVSVLLWLFRK"
     gene            complement(2306..4060)
                     /gene="aceK"
                     /locus_tag="VD737_09750"
     CDS             complement(2306..4060)
                     /gene="aceK"
                     /locus_tag="VD737_09750"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HXW10890.1"
                     /translation="MNLTVSPTSSRDTLVQDCAQLLVDAFADYNADFRAITQRARQRF
                     EERDWRGSQRDAVERIELYDKYVEGTMELMRHRLGDDVHERALWSAIKRRFDEIIDPL
                     PDCEFTKTFFSSVTRKTFGTVGVDPAIEFVALDLDPLASTHTHVETRRYPVRGDTELL
                     AEELLADYRFRTPYRNFERSVKLVSDELKALLANNPPEHRAIESIEVIQEVFYQMTRA
                     YLVGRVNGRGWTAPLVIALRNLESGVVVDAIMLEQGPVSILFSFTRSYFHVDLAHVGE
                     VVKFLKTMLPLKRVSELFTVLGRAKQGKTERYRELFKHLQLSDDKFVLAPGERGLVMI
                     CFTLPSFDVVFKMIRDKFPVQKNILREDVLSKYELVFKHDRAGRLVDAQEFKLIKFPK
                     ARFSPELLAEFEREAAGTVHVEGDDLVIDHMYVERRMTPLNLYLREAPRELAVKAVLD
                     YGQAIRDLAVTNIFPGDLLLKNFGVTRHGRVIFYDYDELCLVTECRFREVPQSQHEED
                     DMRADAWFYVGESDVFPETFINFLGFDPHLRKVFLDAHGEILTADWWRGIQARLREGE
                     LLEVLPYHRHRVRVFSSL"
     gene            complement(4311..5612)
                     /gene="icd"
                     /locus_tag="VD737_09755"
     CDS             complement(4311..5612)
                     /gene="icd"
                     /locus_tag="VD737_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HXW10891.1"
                     /translation="MSTAIKLNVPPQGQKITIENGKLVVPDQPVVPFIEGDGTGPDIW
                     RASVRVLDAAVQKAYGGKRRIHWMEVYAGQKSNDLFNTWLPDETVEACREYLVSIKGP
                     LTTPIGGGIRSLNVALRQMLDLYVCLRPVRWFRGVPSPVKAPEKVDMVIFRENTEDIY
                     AGIEFEAGSPDNDKFKKLFREAFPKLYGKIRFPDTSGIGIKAVSQEGTERLFRAAIEY
                     AIVNKRKSVTIVHKGNIMKFTEGAFRNWAYALAEREFADRVYTWDQWERTKAAKGEKE
                     ANAEQDAAKKAGKVIVKDAIADITLQQVLTRPEEFDVIATMNLNGDYLSDALAAQVGG
                     IGIAPGGNINYVTGHAVFEATHGTAPKYANLDKVNPGSVILSGEMMLRYMGWFEAADA
                     IISAMDKTIGQKTVTYDFARLMEGATEVKCSEFGDRLIANL"
     gene            6269..6676
                     /locus_tag="VD737_09760"
     CDS             6269..6676
                     /locus_tag="VD737_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10892.1"
                     /translation="MRPVALDWIRRRQRSLARGVLALFCLVWLQAALVPCAMALVPGD
                     MAGAPMEHCSYCPPTPGADGDSGAAAVCTYPDGPQVDSRTAALAAPLLLALPTTSFVL
                     EIASLEPHAVPADHRPPDRPGTPLAVSYCRYLK"
     gene            6757..8055
                     /locus_tag="VD737_09765"
     CDS             6757..8055
                     /locus_tag="VD737_09765"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXW10893.1"
                     /translation="MSRSRVRVASLAPLAACSSFLFLAAVTPVAAQDLTLAEAERIAL
                     ERDAMTREMQAQAQAMRERAVMEGQLMDPELRLGAVNVPTDSFSLDEEDMTMLEVGVS
                     QEFPPGRTRQLSRRQMEQLASAMDASAEDRARLVRREVRKLWTQLGYVAAAGAVLDTQ
                     TSWVEQMRQSSRARYASGEGRQLDVLQAGLEAAMLREQQLDLERDDAMYRSQLSFWLG
                     PDEAARARPATLSPRAGLEPLATLEARLAAHPTQADYERRIAAARTAVETAEERRKPG
                     WMLDLSYGFRQGEMPTSMDGDSEPRSDMLSAMVTVDLPLFRRNRQDRAVAAARLDERG
                     LHERHEDHRRELQAMLVEAWNVARRTGELEQFYEDELLQLADQSVQAALLAWGSNRAM
                     IDDVVMARRAATETRMKHLRLAADRALAQHEIDYLAGEER"
     gene            8052..>8787
                     /locus_tag="VD737_09770"
     CDS             8052..>8787
                     /locus_tag="VD737_09770"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXW10894.1"
                     /translation="MNAPLGRLAGTLVLAFLFAACSSKEQPSESVRSAADDTAAEHAI
                     KHTNPKYRCPMHPQIVRDEPGQCPICGMNLVKVEEEEPAATGRAGGGAKEVRISPAVI
                     NNLGVRTEPVVRGSLSRTGQVVGYVEFDERRVQQVRPRAEGWVEGLSVRAMGEPVRKG
                     QLLFTLYSPMLESAQQEYLDALKIGNADLIDASRDRLRALGLDSRTVARLASGGRAAG
                     RVSFHAPIAGVVTELEAREGAMLTPDM"
BASE COUNT         1403 a         2986 c         3027 g         1371 t
ORIGIN      
        1 gtggcgaacc tggcgggatc gtagacgtcg gcgatgacct tgatgtcgtt gagccagtac
       61 tgcacgaccg ccagcacctg gtccgcgggc ggaaacgtcg gcgcgacgtc gggggaatac
      121 ttcgcaaaat cgggccggga cacgaagcgc agcgcggtcg gtggtaccac gtactcggcc
      181 gggtccagga acagcttctg cagctcgtac gccgcgacgt cgtagcgcgg aacgttgttg
      241 aagtcttccg cgccgggctc cgctttgcgc agtttcacac gcagcggctc cgcgccgccg
      301 aatgccacgt cggccttgag agtgatgtcc cccttggcct tgggccggct gatctcggcg
      361 tgcgcgatca cgagcggttc ggcgctgagc agtcgctcga gctcatcgac ggggcgcgtg
      421 acgttggcgt cctgcgcagg tgccgtggca ccgaggagcg ccagcagcgg gatcgtgcac
      481 gcgcgagcga gccgggacac cgaaaccatg cggcacctct cggtggatgg cgggacgtta
      541 ctgcctggtc ggccaatcct agctcgaaac gccgtcgtcg ggcgccgtcg gcgggacgag
      601 gtagaacacg atgtcgaagc cgtccgcgta gaccttgccg aggtactgtg gcctgccgcc
      661 gcagctctgc gccgccgcga ggaaagcggc cgtgtgcttg tgcttcatgc cgtagacgga
      721 gcgctcgtgt tcggcgagtt cgccgcagac gtcctcgtgg tcgacgaact tcgagtattc
      781 gccgcgggcg ggagtgcgcc cgatgtcgat accctcgatg tggcggtcga cgtagacgtt
      841 gaggccatag aacggccgca tgcccacgaa cacgacctcc tcgatcgcgt gcggatcgac
      901 cagccgttcg agctgcgcgg cgaatgcgcg cgcgtcgcga tcgtgcggcc agtatcccag
      961 cagcgctttc agcgcgacca gtgcgaggac gcccgcagcc gcaatgacgg ccagccggcg
     1021 gtcggtcgcc ctcggccacg actggatcgt tctcgccatg acgagggcga gcggcacgaa
     1081 cagtggcagc acgtacaggt gcagcctgga cctggccagg aagaacacga cgagcggcac
     1141 cagcagccag tacgccagca gcaggtcgtc ccgacgccgg gcccgccagt gtgcgcgcag
     1201 gctccgccag gccgcagccg ggccgccggc ggccacgacg gcgagcacgg accatggcag
     1261 cgtccccccc agcagcatgg gaacgtacac ctcgaaaccg ccgtaccact cggaattgcg
     1321 atcgtgcgca gaggtgaaca cgcggtcgta gacttcgtag ccgaggaaat agccgagccg
     1381 gtccggatcc cccgcgacga tcacgccgaa ccaggtgaac gccacgaccg cgaacagcag
     1441 caggccgccc ggcgcaaaca gtggtcgcag ccgtgggcga tcgtgcatcg ccaggaacag
     1501 caccacgccg agcagcggga gcagggcggg cgggcccttg gtcatgaacg cgaggcccca
     1561 gcccagccac atcaacctgt accaggggcg cgcttcgagc gccgagtcgc gcgaccaggc
     1621 ttccacgaac gcgaacatcg ccgcagtctc gaacagcgcc aggatgggat ccgtcgagac
     1681 gatgttcgcc gcgatgcccg gtgccagcga agtgccccac acgacggcgg gcagccaggg
     1741 tcggctccgg caaaaacgcc gtccgagccc gagcaccagc aatcccgtgg cgacgtaggc
     1801 gagggcgctc ggcagccggg ccgcccattc gttgtggccc agggcggcga aactcgcggc
     1861 gatcgcccag tacgtcaccg ggggcttggt caggtgagcg tgctcgccgt ccagcgtcgg
     1921 cacgagccag tcgcccgatt cgtgcatgtt gaccgcggcg gaggtgtaac gtccctcgtc
     1981 gggctcccag atgccgcgcg tgccctggaa ggagaacgcg acgacgagca gcagcacgac
     2041 gagccacgcg ccggctcgcg acgagaccgc cgcggtgtcc cgggctactt cctgaacagc
     2101 caaagcagca cgctcacgac gatgctgagc acgatcatgg tcgtgaacgg cgcaaagaac
     2161 ctgaatccgg gtctgtcgat cacgacgtcg cccggcaggc gcccgaacgg caggcgggac
     2221 agccatggcc acagcagccc ggccagcacg aggagaccgc cgacgaggag tagcaggcgc
     2281 tgcatgcggg gtggctcgcg gtgcgtcaga ggctgctgaa cacgcgcact cgatggcggt
     2341 gatacggcag cacctcgagc aattcgcctt cgcgcagccg cgcctggatg cctctccacc
     2401 agtcggcagt gaggatctca ccgtgcgcgt ccaggaacac cttccgcagg tgcgggtcga
     2461 agccgaggaa gttgatgaag gtctccggga acacgtcgct ctcgccgacg tagaaccagg
     2521 cgtcggcgcg catgtcgtcc tcttcgtgct gcgactgggg tacctcgcga aagcggcatt
     2581 cggtgaccag gcagagctcg tcgtagtcgt agaagatcac gcggccgtga cgcgtcacgc
     2641 cgaagttctt gagcagcagg tcgcccggga agatgttggt gaccgccagg tcgcggatcg
     2701 cctgcccgta gtcgaggacc gccttcaccg ccagctcgcg cggcgcctcg cgcaggtaga
     2761 ggttgagcgg cgtcatgcgt cgctcgacgt acatgtggtc gatcacgagg tcgtcgccct
     2821 cgacgtgcac ggtgccggcg gcttcccgct cgaactcggc cagcagctcc ggcgagaagc
     2881 gcgccttcgg gaacttgatg agcttgaact cctgcgcgtc gacgaggcgg cccgcgcggt
     2941 cgtgcttgaa gacgagttcg tacttcgaca gcacgtcctc gcgcaggatg ttcttctgga
     3001 ccgggaactt gtcgcggatc atcttgaaga ccacgtcgaa cgacggcagc gtgaagcaga
     3061 tcatcaccag cccgcgttcg cccggcgcca gcacgaactt gtcgtccgag agctgcaggt
     3121 gcttgaagag ctcgcggtag cgctcggtct tgccctgctt ggcgcgcccg agcaccgtga
     3181 acagctcgct cacgcgcttc agcggcagca tcgtcttcag gaacttgacg acctcgccga
     3241 cgtgggcgag gtccacgtgg aagtacgagc gcgtgaagct gaacaggatg ctgaccggac
     3301 cctgctcgag catgatcgcg tcgaccacga ctccgctttc gaggttgcgg agcgcgatga
     3361 cgagcggcgc cgtccagccg cggccgttca cgcgccccac gaggtaggcg cgcgtcatct
     3421 ggtagaacac ctcctggatc acctcgatcg actcgatcgc ccggtgctcc ggcggattgt
     3481 tcgcgagcag cgccttgagc tcgtccgaca ccagcttcac gctgcgttcg aagttgcggt
     3541 agggcgtgcg gaagcggtaa tcggcgagaa gctcctcggc cagcagttcg gtgtcgccac
     3601 gaacgggata gcgccgcgtc tcgacgtggg tgtgcgtgct cgcgaggggg tcgaggtcca
     3661 gcgcgacgaa ctcgatcgcg gggtccaccc cgacggtgcc gaaggtcttg cgcgtgacgg
     3721 agctgaagaa cgtcttggtg aactcgcaat ccggcagcgg atcgatgatc tcgtcgaagc
     3781 gccgcttgat cgcagaccat aaggcccgct cgtgcacgtc gtcaccgagc cggtggcgca
     3841 tgagttccat cgtgccttcg acgtacttgt cgtagagctc gatgcgctcg acggcgtcgc
     3901 gctggctgcc gcgccagtcg cgctcctcga atcgctggcg cgcccgctgc gtgatcgcgc
     3961 ggaagtcggc gttgtagtcg gcgaacgcgt cgacgagcag ctgcgcgcag tcctgcacca
     4021 gcgtgtcgcg cgagctcgtc ggggagacgg tgaggttcat cggatgccgg gctcccgtac
     4081 gccgacgagg cctccggagg tgctcgcgcg gcgaccctgc caccgggttt cgatccgggg
     4141 agacgccgtg aagccgtccc tggcggctcg cactcgccct ccctggctcg tgacgctccc
     4201 cggaccgaaa cccggcgtca gcgtccccgc gcagtgtgcg tcgtcgagtc gtcgggcgcg
     4261 cgggaacgcc gcagtcgacc cgcgccgctc agccccgatg gtccgcacga tcagaggttc
     4321 gcgatcagcc ggtcgccgaa ctcgctgcac ttcacctcgg tcgcgccttc catcaggcgg
     4381 gcgaagtcgt aggtgaccgt cttctgcccg atcgtcttgt ccatcgccga gatgatcgcg
     4441 tccgcggcct cgaaccagcc catgtagcgc agcatcatct cgcccgagag gatcaccgag
     4501 cccgggttga ccttgtcgag gttggcgtac ttcggcgcgg tgccgtgcgt cgcctcgaac
     4561 acagcgtgtc ccgtgacgta gttgatgttg ccgcccggcg cgatgccgat gccgccgacc
     4621 tgagcggcga gcgcgtccga caggtagtcg ccgttcaggt tcatcgtcgc gatcacgtcg
     4681 aattcctcgg ggcgcgtgag cacctgctgc agcgtgatgt cggcgatggc gtccttgacg
     4741 atgaccttgc cggccttctt cgcggcgtcc tgctccgcgt tggcttcctt ctcgcccttc
     4801 gcggccttgg tgcgctccca ctggtcccag gtgtagaccc ggtcggcgaa ttcgcgctcg
     4861 gcgagcgcat acgcccagtt ccggaacgcg ccctccgtga acttcatgat gttgcccttg
     4921 tgcacgatcg tcacgctctt gcgcttgttg acgatcgcgt actcgatggc ggcgcggaac
     4981 aggcgctcgg tgccttcctg agagacggcc ttgatgccga tgcccgaggt gtcggggaac
     5041 cggatcttgc cgtagagctt cgggaaggcc tcgcggaaca gcttcttgaa cttgtcgttg
     5101 tcggggctgc ccgcctcgaa ttcgatgccg gcgtagatgt cctcggtgtt ctcgcggaag
     5161 atcaccatgt cgaccttttc tggcgccttg acgggggagg gcacgccgcg gaaccaccgg
     5221 acgggccgca ggcagacgta gaggtcgagc atctgtcgca gcgcgacgtt cagcgagcgg
     5281 atgccgccgc cgatcggggt ggtcagcggg cccttgatcg acacgaggta ctcgcggcag
     5341 gcctcgacgg tttcgtcggg cagccaggtg ttgaagaggt cgttggactt ctggcccgcg
     5401 tagacctcca tccagtggat ccggcgcttg ccgccgtagg ctttctgcac ggcggcgtcg
     5461 aggacgcgca cggaggcgcg ccagatgtcg ggaccggtgc cgtcgccctc gataaacggg
     5521 acgacgggct gatcggggac gacgagcttg ccgttctcga tcgtgatctt ctggccctgc
     5581 gggggcacgt tgagcttgat cgcagtggac atcgatgcgg ctccggggct gcttggatcg
     5641 cgaaaaatcg gggcccggat gctagcacag cgcccctcgc cggagcggcc cggggccggt
     5701 acgcaagcgg ccgggccgac gacgcatcgg cgttcccacg cgcccgttcc gcagttgccg
     5761 gcggttgagg agagcattcg ttgccggcgc gcccggttcc gcacgggtcg ggcagacacg
     5821 ccgtaaaccc gtccctgggg gctcgcactc gccatccgtg gctcgtggcg gtctgcctga
     5881 ccccgtccgg accgggcacg cctgcgccgg tgatggctga ggcgcgctgg acatcgacgc
     5941 cagttgcaac acgtgcgccg cgatgtagcg cccgaagacg gttaagttga cgccggccgc
     6001 cgcgagggct gccgccggat ttcgaccggg gagcgtcacg agccagggat ggcgagtgcg
     6061 agccgcgagg gaccgctctt acggcgtctc cccggctcga aatgcggcgg acagccctct
     6121 gcggccgatg ctgcgcgcct gccagcggtc actcagcccc acgaccgcta gggatttgcc
     6181 gcaagtttcg acgcccgcgg agcattttgc ttgccgcggc ggtctgatcg gctgtacgtt
     6241 ccgctctcct tttggacggt gtctcgaatt gcgccccgtg gccctcgact ggatccgcag
     6301 acgacagcgc tcgctcgccc gtggcgtgct cgcgctgttc tgcctcgtct ggctgcaggc
     6361 cgcgctggtc ccgtgcgcga tggcgctcgt gcctggcgac atggccgggg cgccgatgga
     6421 gcactgctcc tactgccccc cgacgcccgg cgccgatggt gattccggcg ccgccgccgt
     6481 ctgcacgtat cccgacggcc cgcaggtcga ctcgcggacg gccgcactcg cggcccccct
     6541 gctgctggcc ctgccgacca cgagcttcgt gctcgagatc gcttcgctcg aaccgcacgc
     6601 cgtacccgcc gaccaccggc cacccgaccg ccccggcacg ccgctcgcgg tcagctactg
     6661 ccgctacctg aaatagccgg attccgctcg ttgccttcgt cgcgcgccac gtgaccgtgg
     6721 cggcggcgcc tgccgtcaac gccggagtcc gtctttatgt cccgtagccg tgtccgtgtc
     6781 gcgagcctcg caccgctcgc agcctgcagt tccttcctct tcctcgccgc cgtcacgccg
     6841 gtcgcggcgc aggacctcac gctcgcggag gccgagcgca tcgcgctcga gcgtgacgcc
     6901 atgacccgcg aaatgcaggc gcaggcccag gccatgcgcg agcgcgccgt catggaaggc
     6961 cagctgatgg atcccgaact gcgcctgggc gccgtcaacg tcccgaccga ctcgttcagc
     7021 ctcgacgagg aggacatgac gatgctcgaa gtcggcgtga gccaggaatt cccccccgga
     7081 cggacgcgcc agctgtcgcg acgacagatg gaacagctgg cgtccgcgat ggacgccagc
     7141 gccgaggatc gcgcgcggct ggtacgccgc gaggtgcgca agctctggac gcagctcggc
     7201 tacgtcgcgg cggccggggc ggtgctcgac acgcagacgt cgtgggtcga acagatgcgg
     7261 cagtcgtccc gcgctcgtta cgcctcgggc gaaggcaggc agctcgacgt gctgcaggcc
     7321 gggctcgaag ccgcgatgct gcgcgaacaa cagctggacc tcgagcgcga cgacgcgatg
     7381 taccgctcgc agctctcgtt ctggctcggt ccggacgaag ccgctcgcgc acgtccggcc
     7441 acgctctccc cgcgcgccgg actcgagccg ctcgcgacgc tcgaagcgcg gctcgcagcc
     7501 cacccgacac aggcggacta cgagcgtcgc atcgcggcgg cgcgcacggc ggtggagacc
     7561 gcggaggaac gacgcaagcc gggctggatg ctcgacctga gctatggctt ccggcagggc
     7621 gaaatgccga cgtccatgga cggcgactcc gagccacggt cggacatgct gagtgcgatg
     7681 gtcacggtgg acctgccgct gttccgcagg aaccgccagg accgcgcggt ggccgccgcg
     7741 cgtctcgacg agcgcggcct ccacgagcgg cacgaggatc accgtcgcga gttgcaggcg
     7801 atgctggtcg aggcctggaa cgttgcacgg cggacgggtg aactcgagca gttctacgag
     7861 gatgagctgc tgcagctcgc ggaccagtcc gtgcaggcgg cgctgctcgc gtggggctcc
     7921 aaccgcgcga tgatcgacga cgtcgtcatg gcacgtcgcg ccgccaccga aacccgcatg
     7981 aagcatctgc gcctcgccgc cgaccgcgca ctggcgcagc acgaaatcga ctaccttgca
     8041 ggtgaagaac gatgaacgct cccttgggcc gcctggccgg cacgctcgtg ctcgccttcc
     8101 tgttcgctgc ctgctccagc aaggagcagc cgagcgaatc cgtccgaagc gcggccgacg
     8161 acacggcggc cgagcacgca atcaagcaca ccaacccgaa gtaccgctgc ccgatgcacc
     8221 cgcagatcgt gcgggacgag cccggacaat gcccgatctg cggaatgaac ctggtgaaag
     8281 tcgaggagga ggagcccgcc gcgacgggcc gcgccggcgg tggcgcgaag gaagtgcgga
     8341 tctcgccggc cgtgatcaac aacctcggcg tgcgcaccga gcccgtcgtg cgcggcagcc
     8401 tgtcgcgcac cggccaggtc gtcggttacg tcgaattcga cgaacgccgc gtgcagcagg
     8461 tgcggccacg cgccgaaggc tgggtcgaag ggctgtccgt gcgcgccatg ggcgagccgg
     8521 tgcgcaaggg ccagctgctg ttcacgctgt actcgccgat gctcgaaagc gcgcagcagg
     8581 aatacctcga cgcactgaag atcggcaacg ccgacctgat cgacgcctcg cgcgatcgcc
     8641 tgcgggcact cgggctcgat tccaggacgg tcgcgaggct ggccagcggg ggtcgggccg
     8701 ccgggcgcgt gtcgttccac gcgccgatcg cgggagtggt caccgaactc gaggcgcgcg
     8761 aaggcgccat gttgaccccg gacatgg
//
LOCUS       DASPFR010000369         3945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1574519, whole genome shotgun sequence.
ACCESSION   DASPFR010000369 DASPFR010000000
VERSION     DASPFR010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574519"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..423
                     /locus_tag="VD737_09775"
     CDS             79..423
                     /locus_tag="VD737_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HXW10895.1"
                     /translation="MELIVSLAIGVLAGSGVWLLLRPRTFQVVLGLAMLSYAVNLFIF
                     VSGRLTVDRAPIIRGLPVDPSAYADPVPQALVLTAIVISFATTALFLVLLLAARGLTG
                     TDHVDGREPGDK"
     gene            420..2051
                     /locus_tag="VD737_09780"
     CDS             420..2051
                     /locus_tag="VD737_09780"
                     /inference="COORDINATES: protein motif:HMM:NF009309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HXW10896.1"
                     /translation="MIALLHHLMVVPVLVPLVVGAALLFLGEQRRARIALAVGSLIVQ
                     SLAAATLLYLTTDSMPHMWTEGVGVYALGAWPSPFGIVFVVDRLAALMLVLTALIGFS
                     ALVYSLAYWDRVAPAFHSMLQFLLMGLNGAFLTGDLFNLFVFFEVLLAASYGLLLHGA
                     GAVRVKASLHYVTVNLVASFLFLVGIAMVYGITGTLNMADLPGRIAQLDPGDRGLFGA
                     GAAVLGGAFLIKAGVWPLNFWLPAAYTAASSPVGALFAMMTKVGVYAALRVGTLIASA
                     DVETSFIDVGLFYGGAATVVFGVVGTISAHQLGRIVSFSVITSSGIMFMALGLRLQSL
                     TAPVMLYLASSALATSAFFMLNGLAQRTRNVPTREAADVAPLPDVTYHGFGERPADPL
                     SPTDEVGFAIPAAMAFLGMAFVCCVLLVVGLPPLSGFLAKFALLRAAAGPNLLVDGAA
                     YPATFCAALVISGIAALVAFTRSGLRLFWSVSAQRTPRLRVIEVAPTSALILACLALV
                     VAADPVMTYLESAARSLHAPQVYVRVVADTTGALP"
     gene            2048..2527
                     /locus_tag="VD737_09785"
     CDS             2048..2527
                     /locus_tag="VD737_09785"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HXW10897.1"
                     /translation="MNRPAWLLGLGLVLGWMVLNDGYTPGHLLFAVLLATLLMRGVHR
                     LRPTQPRVRRLGIFLPLIAHVLVDIVRSNVAVARFAFRPGLPRRSGFVEVPIELRDPH
                     GLSVLAAIVTATPGTAWAGVSDDGRVLRLHVLDVAEGDELVRHVQERYERPLRRIFE"
     gene            2524..2808
                     /locus_tag="VD737_09790"
     CDS             2524..2808
                     /locus_tag="VD737_09790"
                     /inference="COORDINATES: protein motif:HMM:NF004812.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HXW10898.1"
                     /translation="MMNEVFGYAVWFVQVCLVVAMLNSTVRLFIGPAAQDRVLAFDAM
                     YLNGMLLLLVLGIRSRSTAYFDIALLIALFGFLGTMALARFLMRGEVIEP"
     gene            2805..3164
                     /gene="mnhG"
                     /locus_tag="VD737_09795"
     CDS             2805..3164
                     /gene="mnhG"
                     /locus_tag="VD737_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HXW10899.1"
                     /translation="MTMQTLPLWVTVPGTVLLVFAGVAILAGSLGLLRFPDFFSRLHG
                     PSTTATGGAAAVLLTSMLCSAAIAGRPMFHELVILLLLLGTTPVTSIVMLQAALYRNR
                     ARLSRDAGDAQGPLDAP"
     gene            complement(3140..>3945)
                     /locus_tag="VD737_09800"
     CDS             complement(3140..>3945)
                     /locus_tag="VD737_09800"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HXW10900.1"
                     /translation="RVNPASGEITTIAQGLPPALLPVGGAIDVAFLDGVAYVLVTLVG
                     APFGSGVDGIYRVDGDGQFTVVADIGAFAASHPPQTTFALPQGVQYSIEAHRGGFLVA
                     DGHHNRILRVELDGSITVAHAFGNVVPTGLETWGDTVYVARAGAIPHLPEEGRILAIE
                     SRSGEVSEVASGAPLLVDVERGRGRTLFGLAQGHWSPGQMDGTPADPDTGSLVRVNDQ
                     GGFDVVATGLDRPTSLEVIGNDAYVITLSGTVLRVPDIASAPYGASSGP"
BASE COUNT          482 a         1391 c         1351 g          721 t
ORIGIN      
        1 ccagtcgctg cgccggccgc ggcggccctc gatgcggccg gacgaggtcc acacggcgct
       61 cgccgaggcc gaacgctgat ggaactgatc gtctccctgg ccatcggtgt gctggcaggc
      121 tccggcgtgt ggctgctgct gcggccgcgc acgttccagg tcgtgctcgg gctcgcgatg
      181 ctgtcgtacg cggtcaacct cttcatcttc gtttcgggcc ggctcaccgt cgaccgggca
      241 ccgatcatcc gcggcctgcc ggtcgacccg tcggcctatg ccgacccggt gccgcaggcc
      301 ctcgtgctga ccgcgatcgt gatcagcttc gcgacgacgg cgctgttcct ggtgctgctg
      361 ctggccgcgc gcggcctgac gggcacggac cacgtcgacg gacgtgagcc gggggacaag
      421 tgatcgccct gctgcaccac ctgatggtcg taccggtgct cgtgccgctc gtggtcggcg
      481 cggcactgct gttcctgggt gaacagcgcc gcgcccgcat cgcgctcgcc gtcggctcgc
      541 tgatcgtgca gtcgctcgcc gcggccacgc tgctgtacct gacgaccgac tcgatgccgc
      601 acatgtggac cgaaggtgtc ggggtctacg cgctcggcgc ctggccctca ccgttcggca
      661 tcgtcttcgt cgtcgaccgg ctcgccgcac tgatgctcgt gctgacggcg ctgatcggtt
      721 tctcggcgct cgtgtactcg ctcgcctact gggaccgcgt ggccccggcg ttccattcga
      781 tgctgcagtt cctgctgatg gggctcaacg gcgcgttcct gaccggcgac ctcttcaacc
      841 tgttcgtgtt cttcgaagtg ctgctcgcgg cctcgtacgg actgctgctg cacggcgccg
      901 gggcggtgcg tgtcaaggcg agcctgcact acgtcacggt gaacctggtc gcgtcgttcc
      961 tgttcctggt cgggatcgcg atggtctacg gcatcaccgg cacgctcaac atggcggacc
     1021 tgcccggtcg catcgcgcag ctcgacccgg gcgatcgcgg gttgttcggt gcgggggcgg
     1081 ccgtgctggg cggcgcgttc ctgatcaagg caggcgtctg gccactgaac ttctggctgc
     1141 ccgcggcgta cacggccgcg agctcaccgg tcggcgccct gttcgcgatg atgaccaagg
     1201 tcggcgtcta cgcggcgctg cgggtcggta cgctgatcgc ctccgccgac gtcgagacct
     1261 cgttcatcga cgtggggctg ttctacggcg gcgccgcgac ggtcgtgttc ggcgtcgtcg
     1321 ggacgatctc ggcgcaccag ctcggccgca tcgtcagttt ctcggtcatc acctccagcg
     1381 ggatcatgtt catggcgctc gggctgcggc tgcagtcgct gacggcgccg gtgatgctct
     1441 acctggccag ctccgcgctc gcgaccagtg ccttcttcat gctcaacggc ctggcgcagc
     1501 gcacgcgcaa cgtgccgacg cgcgaggcgg cggacgtcgc gccgctgccc gatgtcacct
     1561 accacggctt cggcgagcgc ccggccgacc cgttgtcgcc gaccgacgag gtcggcttcg
     1621 cgatcccggc ggccatggcg ttcctcggca tggccttcgt ctgctgcgtg ctgctcgtgg
     1681 tcggcctgcc gccgctgtcg ggcttcctgg ccaagttcgc gctgctgcgc gcggccgcgg
     1741 gtcctaacct gctcgtcgac ggtgccgcct acccggccac gttctgcgcg gcgctcgtga
     1801 tctccggcat cgcggcgctc gtcgccttca cgcgcagcgg attgcgcctg ttctggtcgg
     1861 tgagcgcgca gcgcacgccg cggctgcgtg tgatcgaagt cgcgcccacc tccgcattga
     1921 tcctcgcctg cctggcgctg gtcgtggcgg cggaccccgt catgacttac ctcgagtcgg
     1981 cggcgcgctc gctgcacgcc ccacaggtgt acgtgcgggt cgtcgccgac acgacgggag
     2041 cgctgccttg aatcgcccgg cgtggctgct cggactgggc ctggtcctgg gctggatggt
     2101 gctcaacgac ggctatacgc ccggccacct gctgttcgcc gtcctcctgg cgactctgct
     2161 gatgcgtggc gtccatcgtc tgcggccgac ccagccccgc gtgcggcggc tggggatctt
     2221 cctgccgctc attgcgcacg tgctcgtgga catcgtccgg tccaacgtcg cggtggcccg
     2281 tttcgcgttc aggcccgggc tgccgcgacg gagcggcttc gtcgaggtgc cgatcgagct
     2341 gcgcgacccg cacggactgt cggtgctcgc ggcgatcgtg accgcgacgc caggtaccgc
     2401 ctgggccggg gtgtcggacg acgggcgcgt gctgcggctc cacgtactcg acgtcgcgga
     2461 aggcgacgag ctggtacgcc acgtccagga gcgctacgag cgaccgctgc ggaggatctt
     2521 cgaatgatga acgaggtgtt cggctacgcg gtctggttcg tgcaggtgtg cctggtcgtg
     2581 gccatgctca attccaccgt gcgcctgttc atcgggcccg ccgcacagga ccgcgtgctc
     2641 gcgttcgacg cgatgtacct gaacgggatg ctgctgctgc tcgtgctcgg catccgttcc
     2701 cgctccacgg cgtatttcga catcgcgctg ctgatcgcgc tgttcgggtt cctcggcacg
     2761 atggcgctgg cgcgattcct gatgcgtggc gaggtgatcg aaccatgacc atgcagaccc
     2821 tgcccctctg ggtgacggtc ccgggcaccg tgctgctggt gttcgcaggg gtggcgatcc
     2881 tcgcgggctc gctcggcctg ctgcgcttcc ccgacttctt ctcgcgcctg cacggcccgt
     2941 ccaccacggc gacggggggc gccgccgcgg tgttgctcac gtcgatgctg tgctctgccg
     3001 cgatcgcggg gcggcccatg ttccacgaac tggtgatcct gctgctgctg ctcggcacca
     3061 cgcccgtcac gtcgatcgtg atgctgcagg cggcgctgta tcgcaaccgg gcgcgcctgt
     3121 cacgcgatgc cggcgacgct cagggaccgc tcgacgcgcc gtagggtgcg ctcgcgatgt
     3181 cgggcactcg caggactgtg ccagacagcg tgatcacgta cgcatcgttc ccgatcacct
     3241 cgagcgacgt cggacgatcg agcccggtcg cgaccacgtc gaagccaccc tggtcattca
     3301 cgcgcaccag cgagccggta tcgggatcgg cgggcgtccc gtccatctgg ccgggcgacc
     3361 agtggccctg tgccagaccg aagagcgtgc ggccgcgacc gcgctcgacg tcgaccagca
     3421 gcggtgcgcc ggacgcgact tcgctgacct ccccggagcg cgactcgatg gcaagaatgc
     3481 ggccctcctc cggtagatga gggatggctc ccgcacgggc gacatagacc gtatcgcccc
     3541 aggtctcgag tccggtcggc accacgttgc cgaacgcgtg cgcaaccgtg atcgacccgt
     3601 ccagctcgac ccgcaggatc ctgttgtgat ggccgtccgc gaccaggaac ccaccgcgat
     3661 gagcttctat cgaatactgc acgccctgcg gcagtgcgaa ggtcgtctgt ggcggatggc
     3721 tcgcggcaaa cgcaccgatg tcggcgacga cggtgaactg cccgtcgccg tccacgcggt
     3781 agattccatc gacgccgctg ccgaacgggg cgcctacgag cgtgaccagc acgtaggcga
     3841 ctccgtccag gaaagcgacg tcgatggcac ccccaacagg cagcagcgca ggcgggaggc
     3901 cctgcgcgat cgtggtgatc tccccgctcg cagggttgac gcggg
//
LOCUS       DASPFR010000370         3979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1452280, whole genome shotgun sequence.
ACCESSION   DASPFR010000370 DASPFR010000000
VERSION     DASPFR010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452280"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1605)
                     /locus_tag="VD737_09805"
     CDS             complement(<1..1605)
                     /locus_tag="VD737_09805"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXW10901.1"
                     /translation="MPTRTSLARVGRFLRRAACVATSSLIPVTGALAVDSAQDPSAAA
                     VATATTQPPGRPRIGLVLSGGGARGAAHVGVIRALEQMQVPVDAVAGTSMGAVVGGLY
                     AAGLTGEEIERVFQELDWQDMIRDRAPRRDLAYRRKQDDRNILARGTLGVSAEKGIEL
                     PLGLVGGQKISQALRTATARVSDVQDFDRLPTPFRALATDLETGEPVVLGQGDLVTVL
                     RASMSAPGVLTPVEVDGRLLVDGGLVDNLPVELARSMGVDRLIVVDVSFPLAPRDELS
                     SAFDIANQMIGIMVRRGTLESREDLEPGDVVIEPDLGAMTALDFSRMPQVMEIGRKAA
                     LDARDGLGSLALADAEYQQYAAARERAAEPSVRIAFVRPSERSQAEARRVEAVFGDLV
                     GRKLDSPEVQRRLDTQYRFDQFESVDYRVVGNERERGIEIDLRRKSWGPSFLRLGVGV
                     ENDYEGGATANAAARLWITGLNWLDAEWTLDAQIGEEPLFRTEFYQPLSLRVPVFVAP
                     AFRYEMESRPVVNAAGQRIARYRVRDT"
     gene            1724..3826
                     /locus_tag="VD737_09810"
     CDS             1724..3826
                     /locus_tag="VD737_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXW10902.1"
                     /translation="MPQLPARPLHHRPRGWALLLLLPGSALAAPVPELEEVVVTAQRR
                     AVPTLATPLSIGRVGESAIELVGATHSSEVLNRVPGAMVQRGSGQESLTAIRSPVLTG
                     AGSCGAFLLLENGVPVRPVGFCNVNGMLEINTEQADAIEIVRGTGSALYGSNAVHGTI
                     NVLQAEPRGLPETALDLGAGEDGYGRVAFAATRDGAGLDAGVKAFYAHDGGWRDASGF
                     DEVKLNATLVVGDDPDSAGRFDLAATNLNQETAGFVLGKNAYRDEALSATNPNPEAYR
                     DAHAVRLTGLLRPQLDWPGRLELRPYARTSRMDFLQHFLLGQPVEKNGQESAGLMSTF
                     TWDLPERWSLATGVDLEWADSFLLEQQDGPTTEGSPAQNVIRPAGKHYDYTVTSTVAA
                     AYGQLEYALTDTFHVGAGARVERVGYDYDNRMIAGNTDENGVPCTPTGCLYSRPADRE
                     DTFTNVAPKLSVRWEARDGVVLYANVSRGFRPPEMTEVYRLQREQLVADLDSERIDSV
                     ELGVKAALAAWRLEVAAFDMRKDEVILRDSNGFYVSDGRTSHEGVEYQLSWQPTDWLL
                     AGVGGTFARHRYEFSKAVEGGETITRGNDVDTAPRELLRASLELRPLAALSAEAEWLM
                     IGDYFVDASNQHRYPGHEVLNLRAAWDVAPRWTAILRVNNALDRRYADRADFAFGEYR
                     YFPGRPRSIFAEVSWRTE"
BASE COUNT          612 a         1397 c         1396 g          574 t
ORIGIN      
        1 ggtgtcgcgc acccgatacc gcgcgatccg ctgtccggcc gcgttgacga ccggtcgcga
       61 ttccatctcg tagcggaagg ccggcgcgac gaacacgggc acgcgcagcg acagcggctg
      121 gtagaactcc gtgcggaaca gcggctcctc gccaatctgc gcgtcgagcg tccactccgc
      181 gtccagccag ttgagcccgg tgatccacag gcgggccgct gcgttcgcgg tcgcgccgcc
      241 ctcgtagtcg ttctcgacgc ccacgccgag ccgcaggaag ctcggtcccc acgacttgcg
      301 acgcaggtcg atctcgatgc cacgctcccg ttcgttgccc acgacccgat agtcgaccga
      361 ctcgaactgg tcgaaccgat attgcgtgtc gagacgacgc tgcacctcag gcgagtcgag
      421 tttcctgccg accaggtcgc cgaaaacggc ttcgacgcgc cgcgcctccg cctgcgagcg
      481 ctcactgggc cgtacgaatg cgatgcgcac cgacggctcg gcggcgcgct cgcgggccgc
      541 ggcgtactgc tggtactcgg cgtcggccag cgcgagcgat ccgagtccgt cgcgcgcgtc
      601 cagggccgcc ttgcgcccga tctccatgac ctgcggcatg cgcgagaagt ccagcgccgt
      661 catcgcgccg aggtccggct cgatcaccac gtcgccgggt tcgaggtcct cgcgcgactc
      721 gagcgtcccg cgccggacca tgatgccgat catctggttg gcgatgtcga aggccgagct
      781 caactcgtcg cgaggcgcga gcgggaaact gacgtcgacc acgatcaggc gatcgacgcc
      841 catgctgcgc gcaagctcga ccggcaggtt gtcgaccagt ccaccgtcga ccagcaggcg
      901 tccgtcgact tcgaccggcg tcagcacgcc gggtgccgac atgctggcgc gcagcacggt
      961 gacgaggtcg ccctggccca gcacgacggg ctcgccggtc tcgaggtcgg tcgcgagcgc
     1021 gcgaaacggc gtcggcagcc ggtcgaagtc ctgcacgtcc gagactcgcg cggtggccgt
     1081 tcgcagcgcc tggctgatct tctgtccccc gaccaggccg agcggaagtt cgatgccttt
     1141 ttccgcgctg acgccgagcg tgccgcgcgc gaggatgttg cggtcgtcct gcttgcgccg
     1201 ataggcgagg tcgcgtcgcg gcgcgcggtc gcggatcatg tcctgccagt ccagttcctg
     1261 gaacacgcgc tcgatctctt cgccggtgag gccggccgcg tagagaccgc cgaccaccgc
     1321 acccatgctc gtgcccgcca cggcgtcgac gggcacctgc atctgctcga gcgcccggat
     1381 cacgccgacg tgcgcggcac cgcgcgctcc gccgccgctc agcacgaggc caatgcgggg
     1441 tcgacccggc ggctgcgtcg tcgcggtcgc cacggccgcc gcggacgggt cctgcgcgga
     1501 atcgacggcg agagcaccgg tgaccggaat gagcgacgac gtggcgacgc atgcggcgcg
     1561 tcgcaggaaa cgtcccacgc gcgcgaggga ggtacgggta ggcatcggcg acgaaagccc
     1621 cggtcgtgca gtggcgcgat gttagcaaac gccctcgaag cggcttacgg cgccgcgacg
     1681 ccatggacga taatcgaggg tccgcgtgta cgagtgccgt cgcatgcctc agctgccagc
     1741 ccgccctctc catcaccgtc cacggggctg ggcgctgctc ctgctgctgc cgggcagcgc
     1801 gctcgcggcg ccggtgccag aactcgagga ggtggtcgtc acggcgcagc ggcgggcggt
     1861 gccgacgctg gcgacgccgc tcagcatcgg ccgcgtcggc gaatccgcca tcgaactggt
     1921 cggggcgacg cacagctccg aagtactcaa ccgcgtaccc ggcgcgatgg tgcaacgcgg
     1981 cagcggccag gaaagtctca cggccatccg ctcgccggtg ctgaccggcg cgggttcctg
     2041 tggcgcgttc ctgctgctcg agaacggcgt gccggtgcgg ccggtcgggt tctgcaacgt
     2101 caacggaatg ctcgaaatca acaccgagca ggccgacgcg atcgagatcg tccggggtac
     2161 gggcagcgcg ctctacggct cgaacgccgt gcacggcacg atcaacgtgc tgcaggccga
     2221 gccgcgcggg cttcccgaga cggcgctgga cctgggcgcc ggcgaggacg gctacggacg
     2281 cgtcgcgttc gccgcgaccc gcgacggcgc cggtctcgac gcgggcgtca aggcgttcta
     2341 cgcgcacgac ggcggctggc gcgacgcgtc gggcttcgac gaggtgaaac tcaacgcgac
     2401 cctggtggtg ggcgacgatc cggactccgc gggacgcttc gacctggctg ccaccaacct
     2461 caaccaggaa accgcgggct tcgtgctcgg caagaacgcg taccgcgacg aggcgctgag
     2521 cgcgaccaac ccgaacccgg aagcgtaccg cgacgcgcac gcggtccggc tcaccggcct
     2581 gctccgcccg caactcgact ggccaggccg gctcgagctg cggccctacg cgcggacgtc
     2641 gcgcatggac ttcctgcagc acttcctgct cgggcagccc gtcgaaaaga acggccagga
     2701 gagcgccggg ctgatgagca cgttcacgtg ggacctgccc gagcgctggt ccctcgccac
     2761 gggcgtggat ctcgagtggg ccgactcgtt cctgctcgag cagcaggacg gcccgacgac
     2821 cgaaggctcg cccgcgcaga acgtcatccg gccggccggc aagcattacg actacaccgt
     2881 gacgtcgacg gtggccgcgg cctacgggca gctcgagtac gcgctcacgg acacgttcca
     2941 cgtcggcgcg ggcgcgcgcg tcgaacgcgt cggctacgac tatgacaacc gcatgatcgc
     3001 cggcaacacc gacgaaaacg gcgtgccgtg cacgccgacc ggctgcctct acagccggcc
     3061 ggccgaccgc gaggacacgt tcaccaacgt ggcgcccaag ctgagcgtgc gctgggaggc
     3121 gcgcgacggc gtggtgctct acgcgaacgt gtcgcgcggc ttccgcccgc ccgagatgac
     3181 cgaggtgtac cggctgcagc gtgagcagct ggtcgccgac ctcgattcgg agcggatcga
     3241 ctcggtggag ctcggagtca aggcggcgct cgccgcctgg cgcctcgagg tcgcggcatt
     3301 cgacatgcgc aaggacgagg tgatcctgcg cgactccaac ggcttctacg tgagcgacgg
     3361 gcgcacgtcg cacgagggcg tcgagtacca gctctcgtgg cagccgaccg actggttgct
     3421 ggccggcgtg ggcggcacgt tcgctcgaca ccgctacgag ttctcgaagg cggtcgaggg
     3481 cggcgagacg atcacgcgcg gcaacgacgt cgataccgcg cctcgcgagc tattgcgcgc
     3541 gtcgctggaa ctgcgtccgc tcgcggcgct gtcggccgag gcggaatggc tgatgatcgg
     3601 cgattacttc gtcgacgcga gcaaccagca ccgctatccc ggccacgagg tgttgaacct
     3661 gcgtgctgcc tgggacgtcg cgccgcgctg gacggcgatc ctgcgggtga acaacgctct
     3721 cgaccgacgg tacgccgacc gcgcggattt tgcgttcggc gagtaccgct actttcccgg
     3781 gcggccacgc tcgatcttcg ccgaggtgag ctggcgcacc gagtaagtcc tcacttgcct
     3841 gaattgacgg cggcgcgcgg cgaggcgtag cgtggcccgg ccatgacaac cgttacgcgt
     3901 agggaggatg ccatgctgcg acacgtgcag attctgggtg cggtcggggt gctgtcgacg
     3961 ttgctcgcgg tgggcaccg
//
LOCUS       DASPFR010000371         3195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_598972, whole genome shotgun sequence.
ACCESSION   DASPFR010000371 DASPFR010000000
VERSION     DASPFR010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598972"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VD737_09815"
     CDS             <1..327
                     /locus_tag="VD737_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10903.1"
                     /translation="PQQRVVGWALQFRREAAAAGLDTGRDDAQFLRWFDLMGVQRHLK
                     VGGIFARLWLRDGKPGYLADIPRTLEYAVGSCSRHAEFRQLGELIAQRVLPAVIAGLS
                     TTAPAG"
     gene            338..1072
                     /locus_tag="VD737_09820"
     CDS             338..1072
                     /locus_tag="VD737_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008173380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXW10904.1"
                     /translation="MRAMILAAGRGERMRPLTLERPKPLLQVGGEALIEHHLRALSAA
                     GFREVVVNLSWLGDQIRSALGDGSRHGLAVHYSEEGPEPLETGGGIFRALPLLSPKGV
                     ETFLVLNGDVWMDYPYASLREACAGGLPGKDQAHLVMVPNPPHNARGDFALLDGRMVE
                     PVPGMDPGTAVAERIAALPRYTFSGLGVYHPSLFNGCSDGVFKLAPLLRAAAVQARVG
                     VELFDGDWLDIGTPERLAALNERLAG"
     gene            1171..2238
                     /gene="lipA"
                     /locus_tag="VD737_09825"
     CDS             1171..2238
                     /gene="lipA"
                     /locus_tag="VD737_09825"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF009544.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HXW10905.1"
                     /translation="MSEFKGIPVTTSAAPSTERASGRKYVTPQGFTAIRDGIKASSSP
                     GAAALTPVGRKPSWLRAPMPAGRGFDAVREVVREHRLATVCEEAKCPNIGECWNAGTA
                     TIMLMGEVCTRACRFCAVDTGNPRGWLDADEPANAARTVQLMNLKYVVLTSVNRDDLA
                     DGGAAHFAACVREIKRLNPGTAVEALTPDFQGVLHDVETVVDSGLDVFAQNVETVRRL
                     THPVRDARASYEQTLAVLAHAKRHRRGQGDVLVKTSLMLGLGETGDEIIETMRDLREA
                     GVDLLTLGQYLRPTVHHLPVQRFVRPDEFEAYRRAALELGFVECVAGPLVRSSYRAEQ
                     ALARNNAGLGDGGLAQRSHVG"
     gene            2228..2977
                     /gene="lipB"
                     /locus_tag="VD737_09830"
     CDS             2228..2977
                     /gene="lipB"
                     /locus_tag="VD737_09830"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HXW10906.1"
                     /translation="MSAERPSPGLRPTSPAGELSSTASADPGEVKLLWLGRVEYEPTW
                     RAMQQRTDAATDATPDEIWFLEHPPVFTMGMNARAEHLLAVGDIPVVDIDRGGQVTYH
                     GPGQLVVYPLLDLSRRALGVRALVEGIERAVIGALAQWGIVAHGSRDAPGVYVDGRKV
                     ASVGLRIRRSRTYHGLALNVAMDLEPFGRINPCGYAGLQMTQVSALGGPADVRVVAEA
                     LAPHLLRALGFDAGRPDQSATSTWLQDVSSR"
     gene            complement(2932..>3195)
                     /locus_tag="VD737_09835"
     CDS             complement(2932..>3195)
                     /locus_tag="VD737_09835"
                     /inference="COORDINATES: protein motif:HMM:NF016262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HXW10907.1"
                     /translation="AAEDTLFKFPTDFPVKIMGQASDDFRSLVLGIVTRHFGPLEPHR
                     IEERPSANGRYLSLTCTVHAHSKAELDALYRELTSCSQVLVAL"
BASE COUNT          467 a         1102 c         1157 g          469 t
ORIGIN      
        1 cctcagcagc gcgtggtcgg ctgggcgctg cagttccgcc gcgaagcggc ggctgcgggg
       61 ctcgacacgg ggcgcgacga cgcgcaattc ctgcgctggt tcgacctgat gggcgtgcag
      121 cgccacctca aggtcggtgg aatcttcgcg cggctctggc tgcgcgacgg caagccgggc
      181 tacctggcgg acatcccgcg cacgctcgag tacgccgtcg ggtcgtgctc gcgtcatgcg
      241 gagttccgcc aactcggcga gctgatcgcg cagcgcgtgc tgccggccgt gatcgccggg
      301 ctttccacga ccgcgccggc aggctgagcg tcgaacgatg cgtgcgatga tcctcgcggc
      361 cggtcgcgga gagcgcatgc gtccgctcac gctcgagcgg cccaagccgc tgctgcaggt
      421 gggcggcgag gcgctgatcg agcaccacct gcgcgcgttg tccgccgccg ggttccgcga
      481 agtcgtcgtg aacctgtcgt ggctcggcga ccagatccgc agcgcgctgg gcgatggctc
      541 gcgccatggg ctcgcggtgc actacagcga ggaaggaccc gagccgctcg agacgggggg
      601 cggcatcttt cgcgccctgc cgttgttgag cccgaagggc gtcgagacgt tcctggtgct
      661 gaacggcgac gtctggatgg actatccgta cgcgagcttg cgcgaggcct gtgcgggcgg
      721 cctgccgggc aaggaccagg cgcacctcgt gatggtgccg aacccgcctc acaacgcgcg
      781 cggcgatttc gcgctgctcg acgggcgcat ggtcgagccg gtgccgggca tggacccagg
      841 cacggccgtc gcggagcgga tcgcggcgct gccgcgctac acgttctcgg gcctgggcgt
      901 ctatcacccg tcgctgttca acggctgcag cgacggcgtc ttcaagctgg caccgctgct
      961 gcgcgccgcg gcggtgcagg cgcgcgtcgg cgtcgaattg ttcgacggcg actggctcga
     1021 catcggcaca ccggagcgat tggccgcgct gaacgagcgg ctggccgggt aacgccgaag
     1081 gatctcgacg ccacccgctg gggcccgaat gccgtggacc agctgcgctg accctcgcct
     1141 tcccgcggcg ccgggtacaa tgcgcgtccc atgagcgagt tcaagggcat acccgtcacc
     1201 acgtccgccg cgccgtcgac cgagcgcgcc agcggccgca aatacgtgac gccgcagggc
     1261 tttacggcca tccgcgacgg catcaaggcg tcgagctcgc cgggcgcggc ggcgctgacc
     1321 ccggtcggcc gaaagccgtc ctggctgcgc gcgccgatgc ccgccggtcg cggcttcgat
     1381 gccgtgcgcg aggtcgtccg cgagcaccgc ctcgcgacgg tctgcgagga agccaagtgc
     1441 cccaacatcg gggagtgctg gaacgccggc accgcgacga tcatgctgat gggcgaggtc
     1501 tgcacgcgcg cctgccgctt ctgcgcggtc gacaccggca acccgcgcgg ctggctcgac
     1561 gccgacgagc cggccaacgc ggcgcggacc gtacagctca tgaacctgaa gtatgtcgtg
     1621 ctgacctcgg tcaaccgcga cgacctcgcg gacggtggcg cggcccactt cgcggcctgt
     1681 gttcgtgaga tcaagcgcct gaaccccggc actgccgtcg aagcgctgac gcccgatttc
     1741 cagggcgtgc tgcacgacgt cgagacggtc gtcgacagcg gcctcgacgt gttcgcgcag
     1801 aacgtggaga ccgtgcggcg actgacccac cccgtgcgcg acgcgcgggc gtcgtacgaa
     1861 cagacgctcg cggtcctcgc gcacgcgaag cggcaccgtc gcgggcaggg cgacgtgctg
     1921 gtcaagacga gcctgatgct cgggctcggc gaaaccggcg acgagatcat cgagacgatg
     1981 cgcgacctgc gcgaagccgg cgtcgacctg ctgacgctcg ggcaatacct gcgcccgacc
     2041 gtccatcacc tgccggtgca gcggttcgtg cggcctgacg aattcgaggc gtaccgccgg
     2101 gcggcgctcg agctcggctt cgtggaatgc gtggccgggc cgctggttcg gtcgagctat
     2161 cgcgccgagc aggcgctcgc gcgcaacaac gcgggactcg gcgatggcgg actcgcgcaa
     2221 cgatcgcatg tcggctgaac ggccctcacc cggcctgcgg ccgacctctc ccgcgggaga
     2281 gctgtcctct acggcgagcg cggatcccgg cgaggtgaag ctgctgtggc tcggtcgcgt
     2341 cgagtacgaa cccacctggc gcgcgatgca gcagcgcacc gacgccgcca cggacgcgac
     2401 gcccgacgaa atctggtttc tcgagcaccc gcccgtcttc acgatgggca tgaacgcgcg
     2461 ggcggaacac ctgctcgcgg tcggtgacat cccggtcgtc gacatcgatc gcggcgggca
     2521 ggtcacttat cacggacccg gccaactggt cgtgtacccg ttgctcgacc tgtcacgtcg
     2581 cgcgctcgga gtccgcgcgc tcgtcgaagg catcgagcgc gcggtgatcg gcgcgctggc
     2641 ccaatggggc atcgtcgcgc acggcagccg tgacgcgccc ggggtctacg tggacggacg
     2701 caaggtcgcg agcgtcgggt tgcgcatccg ccgcagccgc acttaccacg gcctcgcgct
     2761 caacgtggcc atggacctgg agccgttcgg gcgcatcaat ccgtgcggat acgcggggct
     2821 gcagatgacg caggtgagcg cgctcggcgg accggcggac gtgcgcgtgg tggccgaggc
     2881 gctcgcgccg cacctgctgc gcgcgcttgg attcgacgcg ggtcgaccgg atcagagcgc
     2941 gaccagcacc tggctgcagg acgtgagctc gcggtagagc gcgtcgagct cggccttgct
     3001 gtgcgcgtgc accgtgcagg tgagcgaaag gtaccggccg ttggcgctcg gccgctcctc
     3061 gatccggtgc ggctcgagcg gaccgaaatg acgcgtgacg atgccgagca cgaggctgcg
     3121 gaaatcgtcc gaagcctgcc ccatgatctt gacggggaag tcggttggaa acttgaacag
     3181 cgtgtcttcg gccgc
//
LOCUS       DASPFR010000372         6043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1819670, whole genome shotgun sequence.
ACCESSION   DASPFR010000372 DASPFR010000000
VERSION     DASPFR010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1819670"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VD737_09840"
     CDS             <1..434
                     /locus_tag="VD737_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10908.1"
                     /translation="RPLFTAEYGERDARFSHDGRWVAYVSHEAGRPEVWVRTISGKPR
                     RIVVSGEGGTQPVWSRDGRLLYFVGLQGQLRSVAVHWGENGNPSFDLPRQPDLPPIGF
                     GHWGTQYDVSPDDSRIYFVRPNDDPAPQEIQVVLGWRELLE"
     gene            complement(442..1164)
                     /locus_tag="VD737_09845"
     CDS             complement(442..1164)
                     /locus_tag="VD737_09845"
                     /inference="COORDINATES: protein motif:HMM:NF009044.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXW10909.1"
                     /translation="MGRIFEVRKHAMFARWNRMAKQFSRIGKDITIAVKKGGPDPNSN
                     PALRRVIQNARAVNMPKDKVESAIKRASGRDAANFEEVMYEGYAPHGVAVLVECATDN
                     PTRTVAAVRNAFNKHGGNLATTGSVAFQFKRMGVFRLNPEGIDQDDLELYLIDHGLDE
                     FGESTGEKGEPQLVARCLFEDFGTLQKALEERGLTPLSAEHEYICTTPTELAEAEATE
                     VLELIDKLEQDEDVQKVYHSLA"
     gene            1400..2017
                     /locus_tag="VD737_09850"
     CDS             1400..2017
                     /locus_tag="VD737_09850"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXW10910.1"
                     /translation="MGLYHHCVFPFLLDRAMASKVLRRPRTRTLARARGRILEVGFGT
                     GMNLAHYPPEVTRIEALDPDVDLDRYSRPRIAAARIDVDFHHFDASRLPFDDANFDTV
                     VCTLTLCSIPDVARALAEIRRVLKAGGEFLFLEHGLAPEPSVARWQRRLTPLQRRLGG
                     GCHLDRDTVRLVTRSGLRLAVRDQYYLRGVPRFAGYMTEGYAVKR"
     gene            2379..3536
                     /locus_tag="VD737_09855"
     CDS             2379..3536
                     /locus_tag="VD737_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXW10911.1"
                     /translation="MRVVVMAVGTLALLTTLAGCGFDRHHDGDVVSHRVGSDYFGAGG
                     SLNLTDPVDGDAFLAGGHVSTASEIQGDLVAAGGEVSVGGAIGDDLYAAGGQVQLDAI
                     VAGNARIGGGDVTVGPATVVTGALTLNGGRVRFEGNAHDYLQANGGEVLIDGTVRGDA
                     EVRAERVEVGPAARIGGRLIVRSERAPVVAEGALIAGGVEFEEADIGVHFDDGEVVRD
                     VTVEHGVGSFLWILGVFVAGVLFTLAFPAYSARAADWIGREPLRSLGLGFVVLVCLPI
                     LGVLLLITIVGIPLALILFMLYLLLLFLGWVTAALFVGRKALALVRSSRPVTTAWRIG
                     ALLAAVIALWLVGQVPFIGGWITFAALVLGIGALVWQGWPRRSPPVATGVA"
     gene            3494..4123
                     /locus_tag="VD737_09860"
     CDS             3494..4123
                     /locus_tag="VD737_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10912.1"
                     /translation="MATPQPSRGDGGRLTDRDGPDPAARRGRQDSVIVVSESRDWPPL
                     SGIEAAGAMLFVLWLLVRASTGDRFVPIVDHANLAFHEAGHLLVGLISDRLSVYGGAL
                     GQLFFPMATAWHFARRRHGLGFALAGGWVCESTLNVATYMGDARAMALPLIGGLDPAT
                     HHDWREILSRWGLLEADTTLAFVTSLVAWAAGIALCFWVMRRWLDGRDA"
     gene            4152..4598
                     /locus_tag="VD737_09865"
     CDS             4152..4598
                     /locus_tag="VD737_09865"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXW10913.1"
                     /translation="MFSTYWPYLLAVAVGAALTVQVGMNATVGRALGSPLWASVANFG
                     VGLVALLLAAFAIAPRLQPASFGQAPAWAWAGGLLGAAYVASATLLGPRIGALALVAF
                     VLAGQLGSALLVDQFGWLGFPRAPVTPTRLIGVVLLVAGALLVLRR"
     gene            complement(4605..5192)
                     /locus_tag="VD737_09870"
     CDS             complement(4605..5192)
                     /locus_tag="VD737_09870"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HXW10914.1"
                     /translation="MARRKAVWWAAVLAGNLAACAVHWDVETYAVPGAEVASRETFYW
                     KGGDFQAAAQLDPTAVAAAQAQVRQAVLEELARKGYREVPSASSADLVASFQVSGMRR
                     FVLEDTTRVGAPSPNTVLSPGEMQPPPASSVPREVPVREGSVIVFLDDPKLNKLAWRG
                     EVAEQIRASSPEQVARIIAQMAREIAREVPARDGR"
     gene            5434..>6043
                     /locus_tag="VD737_09875"
     CDS             5434..>6043
                     /locus_tag="VD737_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXW10915.1"
                     /translation="MKTVLLAYERDQDLNAVESVLQARGHHVVKARTGVEALDAIRGN
                     TPDAVVSDVQLPRLDGFALCRRLREDPSFLHLPFVLHSFRVEGPKYEAFAAEVGAQRF
                     LPRGSTLEELVAAIDETPGSGTMRMPALVPGLLDRREQDRRRLTDLERQLRDLEASNQ
                     QLAAAERVARERAEHEARARAEFAASESVRIRELQLRIRELES"
BASE COUNT          797 a         2051 c         2152 g         1043 t
ORIGIN      
        1 cgagaccgtt gttcacggcg gagtacggtg agcgcgacgc ccgtttctcg cacgacgggc
       61 gctgggtagc gtatgtctct catgaggcgg gcaggccgga ggtatgggtt cggacgatct
      121 cgggtaagcc gagacggatc gtcgtctcag gagaaggggg cacacagccg gtctggagtc
      181 gcgacggcag gcttctgtac ttcgtaggcc tgcagggcca gctgcggagc gtcgcggtgc
      241 attggggaga aaacggcaac ccgtcgttcg acctgccgcg acagcccgat ctcccgccga
      301 tcggctttgg gcattggggc acgcagtacg acgtgtcgcc cgacgacagc cggatctatt
      361 tcgtccgacc gaacgacgat ccggcgcccc aggaaatcca ggtcgtcctc ggctggcgcg
      421 aactcctgga gtgagtggtc gctacgcgag cgagtgatag accttctgca cgtcctcgtc
      481 ctgctcgagc ttgtcgatca gctcgagcac ctcggtcgct tcggcttcgg cgagctccgt
      541 gggcgtcgtg cagatgtatt cgtgttccgc cgaaagtggg gtcaggccgc gttcctccag
      601 cgccttctgc agcgtgccga agtcttcgaa caggcagcgt gccacgagct gcggctcgcc
      661 cttctcgccg gtgctctcgc cgaactcgtc gaggccgtgg tcgatcaggt agagctcgag
      721 gtcgtcctgg tcgatgcctt cggggttgag ccggaacacg cccatgcgct tgaactggaa
      781 cgccacgctg ccggtcgtgg ccaggttgcc gccgtgcttg ttgaacgcgt tgcggaccgc
      841 cgccaccgtg cgcgtggggt tgtcggtcgc gcactcgacc agcaccgcaa cgccgtgcgg
      901 tgcgtagcct tcgtacatga cttcctcgaa gttcgccgcg tcgcgtccgg aggcacgctt
      961 gatcgccgac tcgaccttgt ccttcggcat gttcacggca cgcgcgttct ggatcacgcg
     1021 acgcagggcc gggttcgagt tggggtcggg gccgcctttc ttgaccgcga tcgtgatgtc
     1081 cttgccgatg cggctgaact gcttcgccat ccggttccag cgcgcgaaca tcgcgtgctt
     1141 tctgacttcg aagatgcgtc ccatgagtgc actcggcctg ctggttcgtg gcgtcccgcg
     1201 gggagcgggg agggcgcgca atctacctca gcggcaccgg gatggcactc cgcggacgtg
     1261 gactgggccg cacgctgatt gtcaatgtaa acatattaaa tgagcctgcc cgcaatagcc
     1321 gcaattgcgc agtcctcgag tgaagcttac aaatcgatca tgcagagcga atggctgagc
     1381 tggccggccg ggcgttgacg tgggcctgta ccaccattgc gtattcccgt tcctgctcga
     1441 ccgcgcgatg gccagcaagg tgttgcgccg cccgcggacc cgaacgctcg cgcgggcacg
     1501 tggccgcatc ctcgaggtgg ggttcggtac gggcatgaac ctcgcgcact atccgcccga
     1561 ggtgacccgc atcgaggcac tcgaccccga cgtcgacctc gaccgttact cgcgcccgcg
     1621 gatcgcggcg gcccgcatcg acgtcgattt ccaccacttc gacgcctcgc ggctgccgtt
     1681 cgacgacgcg aacttcgaca ccgtcgtgtg cacgctgacg ctctgctcga tccccgacgt
     1741 cgcgcgggcg ctcgccgaaa tccggcgcgt cctgaaggcg ggcggcgagt tcctgttcct
     1801 ggagcacgga ctcgcaccgg agccgtccgt ggcgcgttgg cagcgccggc tcacgccgct
     1861 ccagcgccgc ctgggcggcg gatgccacct cgaccgcgac accgttcggc tcgtgacgcg
     1921 atccggactg cgcctcgcgg tacgcgacca gtattacctg cgtggcgtgc ctcggttcgc
     1981 cggttacatg accgaaggct acgcagtgaa gcgctgatcg caggcgttgc gggtttgcga
     2041 caccacgtac gattcgccgg taagcaagcg ctttattgcg ttgcaaggtg acgacgcggc
     2101 cgtgtccgcg tacaacggca accgagccgc aagcggggga ccgggcggcg aatgcaggga
     2161 gggcccgggc gggggacgac tgggccgtca cggaagacct cgacacttgg gccgcggtgc
     2221 gcaagcgccg cggctttttt cctggtacgc gacgaatcgt gtcgcgcacg ggccgaactc
     2281 cgccgccccg cgtttcacgc caccggcccc tgtcctagac tgatcgcgat gactttcagg
     2341 cgctcggccg cgagagggcg gccggaggag acgaggacat gagagtggtg gtgatggcgg
     2401 tgggcacgct cgccttgctg acgacgctgg cgggctgcgg cttcgaccgt caccacgacg
     2461 gcgacgtggt gagccaccgc gtcggctcgg attatttcgg cgccggcggc tcgctcaacc
     2521 tgaccgaccc ggtggacggc gatgcgttcc tggccggtgg gcacgtctcg actgccagcg
     2581 aaatccaggg agacctcgtc gccgccggcg gcgaggtgtc cgtgggcggt gcgattggcg
     2641 acgacctcta cgccgccggt gggcaggtgc agctcgatgc catcgtcgcc ggcaacgcgc
     2701 ggatcggcgg cggggacgtc accgtgggac ctgccacggt cgtgaccggg gcgctcacgc
     2761 tcaacggcgg tcgcgtccgc ttcgaaggca acgcccacga ttacctgcag gccaacggcg
     2821 gcgaggtgct catcgacggc acggtgcgcg gcgacgccga agtgcgcgcc gagcgcgtcg
     2881 aggtgggccc ggcagcgcgg atcggcggca ggttgatcgt gcgctccgag cgcgcgccgg
     2941 tcgtggccga gggcgccttg atcgcgggcg gcgtggagtt cgaggaggcc gacatcggcg
     3001 tgcacttcga cgacggcgag gtcgtgcgcg acgtcaccgt cgaacacggc gtcggctcgt
     3061 tcctctggat cctcggcgtg ttcgtcgcgg gcgtgctgtt caccctggcg tttccggcct
     3121 actccgcgcg cgcggccgac tggatcggtc gcgagccact gcgcagcctc ggcctcgggt
     3181 tcgtcgtgct cgtctgcctg ccgatccttg gcgtgctgct gttgatcacg atcgtcggca
     3241 ttccgctcgc gctgatcctg ttcatgctct acctgctgct gctgttcctc ggctgggtga
     3301 cggctgcgct cttcgtcgga cgcaaggcac ttgcgctggt gcgcagttcg cgcccggtca
     3361 ccacggcgtg gcgcatcggc gcgctgctcg cggccgtgat cgcgctgtgg ctcgtcggcc
     3421 aggtgccgtt catcggtggc tggatcacgt tcgcggcgct cgtgctcggg atcggcgcgc
     3481 tcgtctggca gggatggcca cgccgcagcc ctcccgtggc gacgggggtc gcctgacgga
     3541 ccgggacggc ccggatccgg cggcgcgccg cggtcggcag gacagcgtca tcgtcgtcag
     3601 cgagtcgcgt gactggccgc cgctcagtgg aatcgaggcc gccggcgcga tgctgttcgt
     3661 gctgtggctg ctcgtgcgcg cgtccaccgg cgatcgcttc gtgccgatcg tcgaccatgc
     3721 gaacctcgcg tttcacgagg ccggccacct gctcgtcggc ctcatctccg accgcctgtc
     3781 ggtgtacggc ggcgcgctcg gccagttgtt ctttccgatg gcgacggcct ggcatttcgc
     3841 gcgccgacgc catgggctcg gctttgcgct ggcggggggc tgggtctgcg agagcacgct
     3901 gaacgtggcc acgtacatgg gcgacgcccg tgcgatggcg ctgccgctga tcggcgggct
     3961 cgatcccgcg acgcaccacg actggcgtga gatcctgtcg cgctggggcc tgctcgaggc
     4021 cgatacgacg ctggccttcg tcacgagcct cgtcgcctgg gccgccggca tcgcgctgtg
     4081 cttctgggtg atgcgacgct ggctggatgg acgcgacgcc tgaactccgg cgtcgcaccg
     4141 ggagactgcg gatgttctcg acctactggc cctacctgct ggcggtcgcc gtcggcgccg
     4201 cgctcaccgt gcaggtgggg atgaacgcga cggtcggtcg cgcactcggt tcgccgctct
     4261 gggcctcggt ggccaacttc ggcgtgggac tcgtggcact gctgctggcg gcgttcgcca
     4321 tcgcgccgcg tctgcagcct gcgtcgttcg ggcaggcgcc ggcgtgggcc tgggccgggg
     4381 gcttgctggg ggccgcgtac gtcgcctcgg caacgttgct gggaccgcgc atcggcgcgc
     4441 tcgcgctggt ggccttcgtg ctcgccggac aactcggctc ggcgctgctc gtggaccagt
     4501 tcggatggct gggatttccg cgcgcgccgg tcacgccgac gcggttgatc ggcgtcgtgc
     4561 tgctggtggc cggcgcgctg ctggtattgc gccgctgact ctcgtcagcg cccgtcgcgc
     4621 gcgggcactt ccctggcgat ttcccgggcc atctgcgcga tgatccgcgc gacctgctcc
     4681 ggcgaactcg cgcggatctg ctcggcgacc tcgccgcgcc aggcgagctt gttcagcttc
     4741 gggtcgtcca ggaacacgat caccgagcct tcgcgcaccg gcacctcgcg cggcaccgac
     4801 gaggccggcg gcggctgcat ctcgcccggg ctcagcaccg tgttcggtga cggtgcgccg
     4861 acgcgggtgg tgtcctcgag cacgaagcgc cgcatgccgg acacctggaa gctcgccacg
     4921 aggtccgcgc tgctcgccga cgggacttcg cgatagccct tgcgcgcaag ttcttcgagc
     4981 accgcctgcc ggacctgcgc ctgtgcggcc gcgaccgcgg tggggtcgag ctgcgcggcc
     5041 gcctggaagt caccgccttt ccagtagaac gtctcgcggc tggcgacctc ggcacccggg
     5101 accgcgtagg tttcgacgtc ccagtgcacc gcgcacgcgg cgaggttccc cgccaggacc
     5161 gccgcccacc agaccgcctt cctgcgcgcc atgtgacctc cgtcgttgcg ccttacgatt
     5221 gtactcgcgt gccgggccgt cacatcgcgg accggcgcgt gtgacgcacg cctcactcga
     5281 tcgggacggc ggggtacatt ctgtgacgcg cgtcgacggg cacgcggcgc ggattggtgc
     5341 gtcgcgacgg cacgggagca tactcggccg aacaaccagg ggcgtcggcg gccggagtcc
     5401 gcgcgccgcc ccgcagggag cttggaccct tagatgaaga ccgtcctgct ggcctacgaa
     5461 cgcgaccagg acctgaacgc cgtggaaagc gtgctgcaag cgcgcgggca ccacgtcgtg
     5521 aaggcgcgca ccggcgtcga agcgctcgac gcgatccgcg gcaacacacc cgacgcggtg
     5581 gtgagcgacg tgcagttgcc gcgcctcgac ggtttcgcac tctgccggcg cctgcgcgag
     5641 gacccctcgt tcctgcacct gccgttcgtg ctgcattcgt tccgcgtcga gggcccgaag
     5701 tacgaggcgt tcgcggccga agtgggcgcg cagcgcttcc tgccccgcgg ctcgacgctc
     5761 gaggaactcg tcgcggcgat cgacgagacg cccggctcgg ggacgatgcg catgccggcg
     5821 ctggtgcccg ggctgctcga ccgccgcgag caggaccgac gccgcctgac agacctggaa
     5881 agacagctgc gggatctcga ggcgagcaac cagcagctcg ccgcggccga acgcgtcgcg
     5941 cgcgagcgcg cggagcacga ggctcgggcg cgcgcggagt tcgcggcgtc ggaatcggtg
     6001 cgcattcgtg agctgcagtt gcgcatccgc gagctcgaga gtt
//
LOCUS       DASPFR010000373         3324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1819700, whole genome shotgun sequence.
ACCESSION   DASPFR010000373 DASPFR010000000
VERSION     DASPFR010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1819700"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VD737_09880"
     CDS             <1..606
                     /locus_tag="VD737_09880"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXW10916.1"
                     /translation="VLGVAAAQASATAGKTDARRPVRVEIQQDAAGLTITQPMRVKDE
                     VRSDYAEAMRLLAAGQDSTGIAALERVIERAPEATAAYVDLGIACARTNDLARAEANL
                     RRALELNPRHPVTHNELGLVLRRQGQYAAARQSYEAAVKAYPDFHFAHRNLAVLCDVY
                     LGDDACALQHYEAYARLAPEDPDVTKWIADLHNRSRAKEAR"
     gene            603..956
                     /locus_tag="VD737_09885"
     CDS             603..956
                     /locus_tag="VD737_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10917.1"
                     /translation="MTRVMPLLSLLLVSGAVMAEAPAPAAAPPAKPVETREVVASADD
                     PKQAAAPGPKVISGMSILGNQEAPKSLVIVPWKSSEIGDSVGIATLLDDSRRPVDRDV
                     FARELAYYQIKAESK"
     gene            1017..1700
                     /locus_tag="VD737_09890"
     CDS             1017..1700
                     /locus_tag="VD737_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXW10918.1"
                     /translation="MDIFYSIVDFFAKGGAFMYPILVVAAFGVAIAVERYVTLTRMRS
                     TNREAWARIEPVLLKADYDKAREMTGKDETAVGRLLSTGLALQGAVRRRDDVEKGMEE
                     TMMEIIPRLQKRTHYLATFANLATLLGLLGTVSGLIHAFAAVATVNPAEKANLLSASI
                     SEAMNCTAFGLMTAVPLLFIHALLQTKTTELIDTLETVSIRFMNSLAARQTARTQSHL
                     ELVKAAANG"
     gene            1711..2223
                     /locus_tag="VD737_09895"
     CDS             1711..2223
                     /locus_tag="VD737_09895"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXW10919.1"
                     /translation="MSLRHRHARLLRKPAELNLVPMIDIFTVLVTFLLMTAVFSRIAI
                     VELDLPSNASGPAVTEPQFRLEVIVRKAGFELTNGTSVIAAVPLQDGKYDLATLSELA
                     SSLKQDHPDVDDASVLLEPDIAYDDLIQVMDAVRSTETTDEGGAGRRRVALFSNIAVG
                     DAPVVGGTST"
     gene            2220..2747
                     /locus_tag="VD737_09900"
     CDS             2220..2747
                     /locus_tag="VD737_09900"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXW10920.1"
                     /translation="MSMLGRIRRRKEHHLSNREDAELNLIPLIDILSVMVAFLLVYST
                     EVEVVQNTKGVEIPQSTVKAAPNQSVVVMITKDHVFVQGELVASIAQVRDPAQPLIDP
                     LRAVLERPMVAAAGQPTEIDVASREITVLADKSLPYEVVRKVMATCTAADYGKISLAV
                     LEKEKPVHAASPKRA"
     gene            2760..>3324
                     /locus_tag="VD737_09905"
     CDS             2760..>3324
                     /locus_tag="VD737_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10921.1"
                     /translation="MSQTVIPGPGETVDTVAPQDSRALVAELEQARERIEALARDLQA
                     VDGELGALATERAQHRLLDEACRSLGELEALNGSQLFWGDAAASRAGAERVRHVRQHV
                     EAFGQRIGVIEGRRQDLIARIEHEQCRAFLLEDDVFEAQEEEERKRQEWIVEREIGAA
                     ANRALIMPWAKGGEDDRRYRKSLISSLL"
BASE COUNT          579 a         1119 c         1125 g          501 t
ORIGIN      
        1 gtcctcggcg tcgccgccgc gcaggcttcg gctaccgcgg gcaagaccga cgcgcggcga
       61 ccggtccgcg tcgagatcca gcaggacgcc gcgggactca cgatcacgca gccgatgcgc
      121 gtcaaggacg aagtccgcag cgactacgcc gaggcgatgc ggctgctggc ggcgggccag
      181 gactcgaccg gcatcgctgc gctggagcgc gtcatcgagc gtgcgccgga ggcgaccgca
      241 gcgtacgtcg acctgggcat tgcctgcgcc aggaccaacg acctggcgcg tgccgaggcg
      301 aacctgcgcc gcgcgctgga gctcaacccg cggcacccgg tgacgcacaa cgaactcggc
      361 ctggtactgc ggcgacaggg ccagtatgcc gccgcccggc agagttacga ggccgccgtc
      421 aaggcttacc cggatttcca cttcgcgcac cgcaacctcg ccgtcttgtg cgacgtgtat
      481 ctcggcgacg acgcctgcgc tctccaacac tacgaggcgt acgcgcggct cgcgcccgag
      541 gaccccgacg tcacgaagtg gatcgccgac ctgcacaacc gttcccgcgc caaggaggct
      601 cgatgactcg cgtgatgccg ttgctgtcgc tgttgctggt ttcgggcgcg gtcatggccg
      661 aagcgcctgc gcccgccgca gcgccgcccg cgaagccggt cgagactcgc gaggtcgtcg
      721 cgagcgcaga cgacccgaag caggccgcgg ccccggggcc caaggtgatt tccggcatgt
      781 cgatcctcgg caaccaggaa gcgcccaagt cgctggtcat cgtgccctgg aagagttccg
      841 agatcggcga ttcggtcggc atcgcgacac tgctcgacga ttcgcgacgt ccggtcgacc
      901 gcgacgtgtt cgcgcgtgag cttgcgtact accagatcaa ggcggaatcg aaatgagggc
      961 ggggcctgcc gccgcgtgct gagagcgata catcgtcact tcaagaggct gataacgtgg
     1021 acatcttcta ttcgatcgtc gacttcttcg ccaagggcgg agcgttcatg tacccgatcc
     1081 tggtggtcgc cgccttcggc gtcgcgatcg ccgtggagcg ttacgtcacg ctgactcgca
     1141 tgcggtcgac caaccgcgaa gcctgggccc ggatcgagcc ggtgctgctc aaggccgact
     1201 atgacaaggc gcgcgagatg accggcaagg acgagacggc cgtcgggcgg ctgctcagca
     1261 cgggtctcgc attgcagggc gcggtccgtc gacgcgacga cgtcgagaaa gggatggagg
     1321 agaccatgat ggaaatcatc ccccggctgc agaagcgcac gcactacctc gcgacgttcg
     1381 ccaacctggc aaccctgctc ggcctgctcg gcaccgtgag cggcctgatt cacgccttcg
     1441 ccgccgtcgc gacggtcaac ccggccgaga aggcgaacct gctctcggcg agcatctccg
     1501 aggcgatgaa ctgcacggcc ttcggcctga tgacggcggt gccgctcctg ttcatccatg
     1561 cgctgctgca gaccaagacc accgagctga tcgacacgct cgagacggtc tcgatcaggt
     1621 tcatgaattc gctcgcggcc cggcagaccg cgcgcaccca gagtcacctc gagctcgtca
     1681 aagccgcggc caacggctga ggatcgtttc atgtcgctgc gccacagaca cgcgcgcctc
     1741 ctgcgcaagc cggccgagct gaacctcgtg ccgatgatcg acatcttcac ggtgctggtc
     1801 acgttcctgc tgatgacggc cgtgttctcg cgcatcgcga tcgtggagct cgacctgccc
     1861 agcaacgcga gcgggcccgc ggtgaccgag ccgcagttcc ggctcgaagt gatcgtgcgc
     1921 aaggcgggtt tcgagctcac caacggcacc agcgtgattg ccgcagtccc gctgcaggac
     1981 ggcaagtacg acctcgcgac cttgtccgag ctggcttcgt cgctcaagca ggatcacccc
     2041 gacgtcgacg atgcgtcggt gctgctcgag ccggacatcg cctacgacga cctgatccag
     2101 gtcatggacg ccgtgcgcag cacggagacg accgacgaag gcggcgccgg gcgcaggcgc
     2161 gtcgccctgt tctcgaacat cgcggtcggc gacgcgccgg tcgtcggagg aacctcgaca
     2221 tgagcatgct gggccgcatt cgtcgtcgca aggaacacca tctctcgaat cgcgaggatg
     2281 ccgagctcaa cctgattcca ttgatcgaca tcctttccgt gatggtcgcg ttcctgctcg
     2341 tgtactcgac cgaggtcgag gtcgtgcaga acaccaaggg cgtggagatt ccgcaatcga
     2401 ccgtcaaggc ggcaccgaac cagtcggtcg tcgtgatgat cacgaaggac cacgtgttcg
     2461 tgcagggcga gctggtcgcg agcatcgccc aggtccggga cccggcgcag cccctgatcg
     2521 acccgctgcg tgcggtgctc gagcgaccca tggtcgcggc ggccggccag ccgaccgaga
     2581 tcgacgtcgc ctcgcgggag atcacggtgc tcgcggacaa gtccctgccc tacgaggtgg
     2641 tccgcaaggt catggcgacc tgcacggccg ccgactacgg caagatttcg ctggcggtcc
     2701 tcgagaagga aaagccagtc cacgcggcga gcccgaagcg ggcttaaagg cggacgaccg
     2761 tgtcgcaaac cgtcattccc gggcccggcg agaccgtgga caccgtggcc ccgcaggact
     2821 ccagggcgct cgtcgccgag ctcgagcagg cgcgcgagcg gatcgaagcc ctcgcgcgcg
     2881 acctgcaggc cgtcgacggc gagctcggtg ccctcgccac ggaacgtgcg cagcatcgcc
     2941 tgctggacga ggcgtgccgc tcgctcggcg agctcgaggc gctgaacggg tcgcagctgt
     3001 tctggggcga tgcggccgca agtcgcgccg gcgccgaaag ggtgcgacac gtgcggcagc
     3061 acgtcgaagc attcggacag cgcatcggcg tgatcgaagg ccgtcggcag gacctgatcg
     3121 cgcggatcga gcacgagcag tgccgcgcgt tcctgctcga ggacgacgtc ttcgaggcgc
     3181 aggaggagga ggaacgcaag cgacaggagt ggatcgtcga gcgcgagatc ggcgcggccg
     3241 cgaatcgtgc gctcatcatg ccgtgggcga agggcggtga agacgaccga cgttaccgga
     3301 agtcgctgat ctcgtcgctg ctcg
//
LOCUS       DASPFR010000374         2852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1087822, whole genome shotgun sequence.
ACCESSION   DASPFR010000374 DASPFR010000000
VERSION     DASPFR010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087822"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1326)
                     /gene="gspF"
                     /locus_tag="VD737_09910"
     CDS             complement(91..1326)
                     /gene="gspF"
                     /locus_tag="VD737_09910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HXW10922.1"
                     /translation="MPAFEYVAVDAAGRERKGVLEGDTPRHVRQMLRDQSLLPVAVDE
                     VLGEQQQQAAGGARRSRSFTLHRGLGAADLALVTRQLATLVKSALPLEESLLAVSQQT
                     EKPKVRNIMLGVRSKVMEGHTLADSLAEFPNSFPELYRATIAASEQSGHLDAVLERLA
                     DYTENRQQLQSRTLGALLYPVLLFVVCVAIIAFLLVTVVPKVVEVFRNSEAELPLLTQ
                     ALIAGSDFMREWGLVLVVAIAVAAWLFRRWLRVESNRRRWHELLLRTPLVGKIVRGSN
                     TARFARTLSTLTASTVPVLDALRISGEVVTNLPMKGAVADAAVRVREGAPIGRSLGQS
                     RLFPPMMIHLISSGETSGELDTMLERAAQHQERELDAVLQAMVGLLGPLMILVMGGLV
                     LVIVMAMLLPIFELNQLVK"
     gene            complement(1336..2811)
                     /gene="gspE"
                     /locus_tag="VD737_09915"
     CDS             complement(1336..2811)
                     /gene="gspE"
                     /locus_tag="VD737_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HXW10923.1"
                     /translation="MTDPLRPLPFAFAKRHGVLVRGITDTDADVVVRDGASPLALAEV
                     RRHLRRALKLERVPVERFETLLREAYEAGSGTTMDAMGGFEEETDLADLAQDIPEPSD
                     LLESEDEAPVIRLINALLTQAVKENASDIHVEPFENRLVIRFRVDGVLREVLQTKRAV
                     APLIVSRIKVMSRLDIAEKRLPQDGRISLRIAGRAVDVRVSTIPAGHGERVVLRLLDK
                     QAGRLDLNSLGMDPVTQNHIDELIHKPNGILLVTGPTGSGKTTTLYAALDRINDRTRN
                     IMTVEDPIEYYIDGIGQTQVNTRVEMTFARGLRAILRQDPDVVMVGEIRDLETAQIAV
                     QSSLTGHLVMSTLHTNTAVGAVTRLRDMGIEPFLLASSVVGVLAQRLVRVLNPVTREA
                     YTAGEYERRLLNLPDDAPSPTLYRPSSKDPAGSYRGRTGIYELVIVDEHMRTMIHDGA
                     SELDLERYARMRTPGIRDDGRSKVLAGVTTIEEVLKVTRED"
BASE COUNT          416 a          963 c         1023 g          450 t
ORIGIN      
        1 cgcgccgacg aggcgctgag ttgacataac ctgcgaacac ccgatcgcac gcgatcggcg
       61 aaaccgaggc tgttgcgacg cgccggccgt ctatttgacg agctggttga gctcgaagat
      121 cggcagcagc atcgccatca cgatcacgag cacgagcccg cccatcacga ggatcatcag
      181 cgggccgagc agcccgacca tcgcctgcag cacggcgtcg agttcgcgct cctggtgctg
      241 ggcggcgcgc tcgagcatgg tgtcgagctc gccgctggtc tcgccgctcg agatcaggtg
      301 gatcatcatc ggcgggaaga gccggctctg tccgagcgag cggccgatcg gcgcgccttc
      361 gcgcacgcgc acggcggcat ccgccacggc gcctttcatc ggcaggttgg tgacgacctc
      421 tcccgagatg cgcaacgcgt ccagcaccgg caccgtgctc gcggtcagcg tcgacagcgt
      481 gcgtgcgaac cgcgcggtgt tggagccccg cacgatcttg cccaccagcg gcgtgcgcag
      541 cagcagctcg tgccagcggc ggcggttcga ttcgacgcgc agccagcgcc ggaacagcca
      601 cgccgccacg gcgatcgcca cgaccagcac caggccccac tcgcgcatga agtcgctgcc
      661 ggcgatcagc gcctgcgtga gcagcggcag ttccgcctcg ctgttgcgga acacctcgac
      721 caccttgggc acgacggtga cgagcaggaa cgcgatgatc gcgacgcata ccacgaacag
      781 cagcactgga tacagcagcg cgccgagcgt gcggctctgc agctgctggc ggttttcggt
      841 gtagtcggcg agccgctcga gcacggcgtc caggtggccc gactgttcgc tcgccgcgat
      901 ggttgcgcgg tagagctcgg ggaagctgtt cgggaactcg gcgaggctgt ccgccagcgt
      961 gtggccctcc atcacctttg agcgtacgcc cagcatgatg ttgcggacct tgggcttctc
     1021 ggtctgctgc gaaaccgcga gcagcgactc ctcgaggggc agcgccgact tgaccagcgt
     1081 ggcgagctgc cgcgtcacca gcgccaggtc cgccgcaccg aggcctcggt gcagcgtgaa
     1141 cgaccgcgag cggcgcgcgc cgcctgccgc ctgctgctgc tgctcgccga gcacctcgtc
     1201 gaccgcgacc ggcagcagcg actggtcgcg cagcatctgg cgcacgtgac gcggcgtgtc
     1261 tccctcgagg acgcccttgc gttcgcggcc ggcggcgtcg acggcgacgt attcgaaagc
     1321 aggcatggaa gcggctcagt cctcgcgagt gaccttgagg acttcctcga tggtggtcac
     1381 gccggccagc accttggagc ggccgtcgtc acggatgccg ggcgtgcgca tgcgcgcgta
     1441 gcgctcgagg tcgagctcgg aggcgccgtc gtggatcatc gtgcgcatgt gctcgtcgac
     1501 gatcacgagc tcgtagatgc cggtgcggcc tcgatagctg ccggccgggt ccttcgaact
     1561 cggccggtac agcgtgggcg agggggcgtc gtccggcagg ttgagcaggc gccgctcgta
     1621 ctcgccggcg gtgtaggcct cgcgcgtgac cgggttcagc acgcgcacga gccgctgcgc
     1681 cagcacgccc acgaccgagg aggcgagcag gaacggctcg atgcccatgt cgcgcaggcg
     1741 cgtgacggcg cccacggcgg tgttcgtgtg cagggtcgac atgaccaggt ggccggtcag
     1801 cgacgactgc acggcgatct gcgccgtctc gaggtcgcgg atttctccga ccatcacgac
     1861 gtccgggtcc tggcgcagga tcgcgcgcag cccgcgcgcg aacgtcattt cgacacgcgt
     1921 gttgacctgc gtctgcccga tgccgtcgat gtagtactcg atcgggtcct cgaccgtcat
     1981 gatgttgcgc gtgcggtcgt tgatgcggtc gagcgccgcg tacagggtcg tcgtcttgcc
     2041 tgaaccggtc gggccggtga cgagcaggat gccgttcggc ttgtggatca gctcgtcgat
     2101 gtggttctgc gtgaccggat ccatgccgag cgagttcagg tcgagccggc cggcctgctt
     2161 gtcgagcagg cgcagcacca cgcgttcgcc gtgcccggct gggatcgtcg acacgcggac
     2221 gtccacggcg cggccggcga tgcgcagcga gatgcgcccg tcctgcggca ggcgcttctc
     2281 ggcgatgtcc agtcgcgaca tgaccttgat gcgcgagacg atcagcgggg cgaccgcgcg
     2341 cttggtctgc agcacttcgc gcagcacgcc gtcgacgcgg aagcggatca cgaggcggtt
     2401 ctcgaacggc tcgacgtgga tgtcggaggc gttttccttg accgcctgcg tgagcaacgc
     2461 gttgatcaag cggatgaccg gtgcctcgtc ctcgctctcg agcaggtcgg acggttccgg
     2521 gatgtcctgg gccaggtcgg cgaggtcggt ttcctcctcg aacccgccca tcgcgtccat
     2581 ggtcgtgccg gagccggcct cgtaggcctc gcgcagcagt gtctcgaagc gctccaccgg
     2641 gacgcgctcg agcttgagcg cccggcgcag gtggcgacgc acctcggcga gggccagcgg
     2701 cgaggcgccg tcccgcacga cgacgtccgc gtcggtgtcg gtgattccgc gcaccagcac
     2761 gccgtgccgc ttcgcgaacg cgaagggcag ggggcgcagc gggtcggtca cgcggccggc
     2821 ggcggcgagg atggcggctt cggtggcggc gg
//
LOCUS       DASPFR010000375         5447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1332820, whole genome shotgun sequence.
ACCESSION   DASPFR010000375 DASPFR010000000
VERSION     DASPFR010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332820"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VD737_09920"
     CDS             <1..445
                     /locus_tag="VD737_09920"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HXW10924.1"
                     /translation="GGATKNVLAIGAGISDGLGFGANTRVALITRGLAEMMRLGVALG
                     AQKDTFMGLTGLGDLVLTCTDDQSRNRRFGLALAAGKSSEVAQREIGQVVEGVLAAKA
                     VCAVAAAHGVEMPIAEQINRIIYHGAPPRDAVAALMNRAIKSETQ"
     gene            complement(470..961)
                     /locus_tag="VD737_09925"
     CDS             complement(470..961)
                     /locus_tag="VD737_09925"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003171120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HXW10925.1"
                     /translation="MPLDVVLFEPEIPPNTGNVIRLCANTGARLHLVEPLGFRLDARG
                     VRRAGLDYRELAEVAVHRNFATCLASLSAGRPVGEVRWFALTTRGAVGYDAARYEPDD
                     VLVFGPETRGLPADVLAACPEARRLRIPMRAGARSLNLSNAVAIVVYEAWRQFGWSGA
                     TTP"
     gene            1018..1446
                     /locus_tag="VD737_09930"
     CDS             1018..1446
                     /locus_tag="VD737_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008050583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXW10926.1"
                     /translation="MLTVRQLLRRKGQDVWSIDVEEPVLEAIQLMADKHVGALPVTRN
                     GELAGVISERDYARKVILLGRSSAETQVWQIMSSPVVTVAPDQDVRECMQLMTNRRIR
                     HLPVVEQGRMVGVISIGDLVRAVIEEQDYTIEQLERFISG"
     gene            1546..2514
                     /locus_tag="VD737_09935"
     CDS             1546..2514
                     /locus_tag="VD737_09935"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HXW10927.1"
                     /translation="METVLPASLAVEFCGIKLASPIVLLSGCVGFGEEYTRVEGFSNR
                     DVGAIVLKGTTREPRLGNPAHRVYETPLGMLNAIGLQNPGVDVVIDQILPTLDFTETT
                     FFANVCGSTIEDYVEVTRRFDDSPIDAIELNISCPNIKEGGVQFGNSPEMSARVVSAC
                     RAVTTKPLITKLSPNQTDIRENARRCIDAGSDALAVINTVMGMAVDVKTRRPVIGNVQ
                     GGLSGPAIKPIALLKVMQVAEVARPHRVPIIGQGGICNAQDALEFIIAGATTVGVGTA
                     LFYEPHSCKRINAGIADYLAANGMSSVQELVGSLEVQRRAVEACGC"
     gene            complement(2629..4041)
                     /gene="ntrC"
                     /locus_tag="VD737_09940"
     CDS             complement(2629..4041)
                     /gene="ntrC"
                     /locus_tag="VD737_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HXW10928.1"
                     /translation="MNHPLPALDVWIVDDDASIRWVLERALKQAGMTSTAFEDADAAL
                     AALRRGEPDVLVTDVRMPGKSGLDLLDEVRARRPRLPVIVMTAHSDLDNAVAAYQGGA
                     FEYLPKPFDIDQAVDLVRRAASQQQRGVESASESRRIPEMLGHAPAMQEMFRAIGRLS
                     RSSMTVLITGESGTGKELVARALHRHSPRATKPFIALNTSAFTADLLESELFGHEKGA
                     FTGASELRRGRFEQADGGTLFLDEIGDMSPQLQTRLLRVLAEGEFYRVGGQLPVKVDV
                     RVIAATHQDLEARVRDGLFREDLLHRLNVIRIEVPPLRHRREDIPELLQHYLDVAALE
                     LGVAPKVLTEEATRALAAFDWPGNVRQLVNACRRLTVLAAGREIKLTDLPTDLGGAGA
                     YTSTQTPDWSNALASWAQARLLAGGPPLLDEAMPEFERTLLREALKATRGGRQDAAKL
                     LGWGRNTLTRKLKELGMEDL"
     gene            complement(4016..5122)
                     /gene="glnL"
                     /locus_tag="VD737_09945"
     CDS             complement(4016..5122)
                     /gene="glnL"
                     /locus_tag="VD737_09945"
                     /inference="COORDINATES: protein motif:HMM:NF008293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HXW10929.1"
                     /translation="MAELHQGQRPSQAELVALLDGTATSIVWLDADAAVVHLNEPAED
                     LFGVGRNQALGRSMRDLLKGNVELEGVVARARAAAAQYSRREIPFESGPGATPRVLDV
                     TVTPFDPPGRTGGVLVELADTTHHQRITRENALLTQLGGSRAMVRQLAHEIKNPLGGL
                     RGAAQLLERQLREPALHEYTTVIIAEADRLAALVDALLGPGQPPHKQSVNIHEIVQHV
                     GHLLAAEAPTGVKIERDYDPSLPRLRLDRNQIIQALLNLGRNAIQAVGEHGRIVLRTR
                     ALTNASIGSRRYRVVVGIQVEDDGPGVPAELKDTVFYPLVTGRKTGTGLGLAVAQDLI
                     GRHDGLIEFDSRPGRTVFTILLPVDAHEPPIAGA"
     gene            complement(5202..>5447)
                     /locus_tag="VD737_09950"
     CDS             complement(5202..>5447)
                     /locus_tag="VD737_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10930.1"
                     /translation="GNDRLLTYLDGKLLSNENAYEHTLSNLDRGVHSVTSVILDAKGN
                     EKIRSQPVVFHIKQPTAIENPRNRGPAVRPPQPKPGG"
BASE COUNT          825 a         1861 c         1916 g          845 t
ORIGIN      
        1 cgggggcgcc accaagaacg tgctcgccat cggcgcgggc atatccgacg ggctcgggtt
       61 cggggccaac acgcgcgtcg cgctgatcac gcgcgggctc gccgagatga tgcggctcgg
      121 cgtcgcgctc ggcgcccaga aggacacctt catggggctc acgggcctcg gcgacctcgt
      181 gctgacctgc accgacgacc agtcgcgcaa ccggcgcttc ggccttgcgc tcgccgcggg
      241 caagtccagc gaggtcgcgc agcgcgagat cggccaggtg gtcgagggcg tgctcgcggc
      301 caaggcggtc tgcgcggtcg cggctgcgca cggcgtcgaa atgccgatcg ccgagcagat
      361 caaccggatc atctaccacg gcgcgccgcc gcgtgacgcg gtcgctgcgc tgatgaaccg
      421 ggcgatcaag tccgagaccc agtagcaccg ccggcgcatc aggcgcccat cacggcgtgg
      481 tcgctccgct ccacccgaac tgtcgccacg cctcgtagac gacgatggcg accgcgttcg
      541 acaggttcag gctgcgggcg ccggcccgca tcgggattcg caaccggcgg gcctccggac
      601 acgctgccag cacgtcggcc ggcagcccgc gcgtttccgg cccgaacacg agcacgtcgt
      661 cgggctcgta gcgcgccgcg tcgtagccca cggcaccgcg cgtcgtcagc gcgaaccagc
      721 gcacctctcc caccggcctt cctgcagaca acgacgccag gcaggtcgca aagttgcggt
      781 gcacggcgac ctcggccagc tcccggtagt cgagccctgc gcgacgcacg ccgcgtgcat
      841 cgagccggaa gccgagcggc tccacgaggt gcaagcgcgc ccccgtgttg gcgcacagcc
      901 ggatcacgtt gccggtgttg ggcgggatct cgggctcgaa caggacgacg tcgagtggca
      961 tagaatcggc aggcacgttc gcgagcggct cgcggccggc gcacgaggga ttgtcgcatg
     1021 ctgaccgtca ggcaactgct ccgccggaaa ggccaggacg tctggtcgat cgacgtggaa
     1081 gagccggtgc tggaggcaat ccagctcatg gcggacaagc acgtgggcgc ccttcccgtc
     1141 acgcgcaacg gcgaactcgc cggcgtgatc tcggagcgtg actacgctcg aaaggtgatc
     1201 ctgctcggac gatcgtccgc ggagacgcag gtctggcaga tcatgagttc gcccgtggtg
     1261 acggtggcgc ccgaccagga cgtgcgcgag tgcatgcagc tcatgacgaa ccggcgcatc
     1321 cgccacctgc cggtggtcga gcaggggcgc atggtcggag tgatttcgat cggcgacctg
     1381 gtgcgtgccg tgatcgagga acaggattac accatcgagc agctcgagcg tttcatctca
     1441 ggctgaccgc agtccgcctg cagatcccgg tcatggacgg gttcgccgcc gcagggtgcc
     1501 ccgcctcggc gccgccgttt gggaaacacc tatacaatgc cggcaatgga gaccgtccta
     1561 ccggcatcgc tcgcagtcga gttctgcggg atcaagctgg cctcgccgat cgtactgttg
     1621 tccgggtgtg tcggtttcgg cgaggagtac acgcgcgtcg agggcttctc gaaccgcgac
     1681 gtcggcgcga tcgtgctcaa gggcacgacg cgggaaccgc gcctcggcaa ccccgcccac
     1741 cgcgtctacg aaaccccgct cggcatgctc aacgcgatcg ggctgcagaa tcccggcgtc
     1801 gacgtggtca tcgaccagat cctgcccacg ctcgacttca ccgagaccac gttcttcgcc
     1861 aacgtctgcg gctcgacgat cgaggactac gtcgaggtca cgcggcggtt cgacgattcg
     1921 ccgatcgacg cgatcgaact caacatctcg tgtcccaaca tcaaggaggg cggggtgcag
     1981 ttcggcaact cgcccgagat gtcggcgcgc gtcgtgagtg cctgtcgcgc ggtgacgacc
     2041 aagccgctga tcaccaagct ctcgccgaac cagaccgaca tccgcgagaa cgcgcgccgg
     2101 tgcatcgacg caggcagcga cgcgctcgcc gtgatcaaca ccgtgatggg catggcggtc
     2161 gacgtgaaga cgcgacgccc ggtgatcggc aacgtgcagg gcggcctctc cggcccggcg
     2221 atcaagccga tcgcgctgct caaggtcatg caggtggccg aagtcgcgcg acctcaccgc
     2281 gtgccgatca tcggccaggg tggcatctgc aacgcgcagg acgcgctcga gttcatcatc
     2341 gccggcgcga ccaccgtcgg cgtcggcacg gcgctgttct acgagcctca ctcctgcaag
     2401 cgcatcaatg ccggcatcgc cgactacctg gccgccaacg gcatgagctc cgtgcaggag
     2461 ctggtcgggt cgctcgaagt gcagcggcgg gcggtggagg cgtgcggctg ctgacgagag
     2521 tgtctggtgt ctagtgtcta gcggttagtc agaggcggag tgcactctgg acggttggag
     2581 ctgacactgg ctcctactaa ccgctagaca ctgaacacta gacacttctc acaggtcctc
     2641 catccccagc tctttcagct tccgcgtcag cgtgttgcgc ccccagccga gcagcttcgc
     2701 cgcgtcctgg cggccgccgc gcgtggcctt gagcgcctcg cgcagcagcg tccgttcgaa
     2761 ctcgggcatc gcttcgtcga gcagcggcgg accgccggcc agcaggcgcg cctgcgccca
     2821 gctggcgagt gcgttcgacc agtccggggt ctgggtcgag gtgtacgcgc ccgcgccgcc
     2881 caggtcggtc ggcaggtcgg tcaacttgat ctcgcgcccg gcggccagca cggtgagccg
     2941 gcggcaagcg ttgacgagct ggcgcacgtt gcccggccag tcgaacgctg cgagcgcgcg
     3001 cgtggcttcc tccgtcagca ccttcggggc cacgccgagc tcgagcgccg cgacgtcgag
     3061 gtagtgctgc agcagttccg ggatgtcctc gcgtcgatga cgcagcggcg gcacctcgat
     3121 gcggatcacg ttgagccggt gcagcaggtc ttcgcggaac agcccgtcgc gcacgcgcgc
     3181 ttcgaggtcc tggtgtgtcg cggcgatcac gcgcacgtcg accttgacgg gcagctggcc
     3241 gccgacacgg tagaactcgc cttcggcgag cacgcgcagc agccgcgtct gcagttgcgg
     3301 cgacatgtcg ccgatctcgt cgaggaacag cgtgccgccg tcggcctgct cgaaccgtcc
     3361 gcgtcgcagc tcggaggcgc ccgtgaacgc gcccttctcg tgaccgaaaa gctccgactc
     3421 gagcaggtcg gccgtgaacg ctgaggtgtt gagcgcgatg aacggcttgg tcgcgcgcgg
     3481 gctgtggcgg tgcagggccc gggccaccag ctccttgccg gtgccggact ctccggtgat
     3541 cagcaccgtc atcgacgagc gcgacaaacg tccgatcgcg cggaacatct cctgcatcgc
     3601 cggtgcgtgc ccgagcatct ccgggatgcg ccgcgactcc gacgccgact cgaccccgcg
     3661 ctgctgttgc gaagccgcgc gccgcacgag gtcgacggcc tggtcgatgt cgaacggctt
     3721 cggcaggtat tcgaaggcgc cgccctggta ggccgcgacg gcgttgtcga ggtcggagtg
     3781 cgcggtcatg acgatgaccg gcaggcgagg ccgtcgcgcg cgcacctcgt cgagcaggtc
     3841 gagcccgctc ttgccgggca tgcgcacgtc cgtcacgagc acgtcgggtt cgcctcgacg
     3901 cagcgcggcg agcgcggcgt ccgcatcctc gaacgcggtg gacgtcatgc cggcctgctt
     3961 gagtgcacgc tcgagcaccc agcggatcga ggcgtcgtcg tcgacgatcc agacgtcaag
     4021 cgccggcaat gggtggttca tgcgcgtcga ccggcaggag gatggtgaag acggtgcgac
     4081 ccggccggct gtcgaactcg atcaggccgt cgtgtcgccc gatcaggtcc tgcgccaccg
     4141 cgaggccgag ccccgtgccg gtcttgcgac ccgtcacgag cggatagaac acggtgtcct
     4201 tgagttcggc gggcacgccc ggcccgtcgt cctcgacctg gatcccgacg accacccgat
     4261 accgacgact gccgatgctg gcattggtca gcgcgcgcgt gcgcagcacg atgcgaccgt
     4321 gctcgccgac ggcctggatc gcgttgcggc cgaggttcag cagtgcctgg atgatctggt
     4381 tacggtcgag ccgcaggcgc ggcaggctcg ggtcgtaatc gcgctcgatc ttgacgccgg
     4441 tcggggcctc cgcggcgagc aggtgaccga cgtgctggac gatctcgtgg atgttcacgg
     4501 actgcttgtg cgggggctgg cccgggccga gcagcgcgtc gacgagggcc gcgaggcggt
     4561 cagcctccgc aatgatgacc gtggtgtatt cgtgcagcgc cggctcgcgc agctggcgct
     4621 cgagcaactg cgccgcgccg cgcagcccgc cgagaggatt cttgatctcg tgcgcgagct
     4681 gccggaccat cgcgcggctg ccgcccagct gcgtcagcag cgcattctcg cgcgtgatgc
     4741 gctggtggtg cgtggtgtcg gcgagttcga ccagtacgcc cccggtgcga cccggcgggt
     4801 cgaacggcgt gacggtgacg tcgaggacgc gcggcgtcgc gccgggcccg gattcgaacg
     4861 ggatctcgcg ccgggagtac tgcgccgccg cggcacgcgc gcgggcgacg acgccctcga
     4921 gctcgacgtt gcccttcagg aggtcccgca tcgatcgacc gagcgcctgg ttgcgaccca
     4981 cgccgaagag atcctcggcg ggctcgttca ggtgcacgac cgcagcgtcg gcgtcgagcc
     5041 agacgatgga ggtggccgtg ccgtccagca gcgcgacgag ctcggcctgg gagggtcgct
     5101 ggccctgatg gagttcggcc accacgctca ggccctccgg gcccgcatac gcgggagaag
     5161 gtcgcgggac agcttgctgc ccggcgcgat gccggggtgg gtcagccgcc gggcttgggc
     5221 tggggcggcc gcacggccgg cccgcgattt cgcggattct cgatcgccgt cggctgcttg
     5281 atgtggaaca cgaccggctg gctgcggatc ttctcgttgc ccttcgcgtc gaggatcacg
     5341 ctcgtcacgg aatgcacgcc gcgatcgagg ttgctcagcg tgtgctcgta ggcgttctcg
     5401 ttcgacagca gcttgccgtc caggtacgtg agcagccggt cgttgcc
//
LOCUS       DASPFR010000376         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_844289, whole genome shotgun sequence.
ACCESSION   DASPFR010000376 DASPFR010000000
VERSION     DASPFR010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844289"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1370
                     /gene="leuC"
                     /locus_tag="VD737_09955"
     CDS             <1..1370
                     /gene="leuC"
                     /locus_tag="VD737_09955"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004774237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HXW10931.1"
                     /translation="DDGTALIYIDRHLVHEVTSPQAFEGLKLAGRQPWRIESVVATAD
                     HNTPTTGWERGIEGISDPTSRLQIDTLDANMREYGKRAKAYFPFLDRRQGIVHVIGPE
                     QGATLPGMTVVCGDSHTSTHGAFACLAHGIGTSEVEHVLATQCLVTQKMRNMRVSVEG
                     ALPRGVGAKDVVLAIIGRIGTAGGSGYAIEFAGSAIRALSMEGRMTVCNMAIEAGARA
                     GMVAVDDTTLAYLKGRPFAPAGPDWDAAVAYWKTLKSDPDAKFDREVALRAEEIVPQV
                     SWGTSPEMVVPVDGEVPDPEREKDSIRREGMERALQYMGLKPRTRITDIRIDKVFIGS
                     CTNSRIEDLRVAAAVVKGRRVAANVRLAMVVPGSGLVKAQAEKEGLDRVFRDAGFDWR
                     QPGCSMCLGMNADRLDPGERCASTSNRNFEGRQGDGGRTHLVSPAMAAAAAVAGHFVD
                     VRQWR"
     gene            1378..2019
                     /gene="leuD"
                     /locus_tag="VD737_09960"
     CDS             1378..2019
                     /gene="leuD"
                     /locus_tag="VD737_09960"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HXW10932.1"
                     /translation="MEKFTVHTGIVAPLDRGNVDTDQIIPKQFLKSIKKTGYGPNLFD
                     AWRYMDHGEPGMDHARRPRNPNFVLNDPRYKDATILLARKNFGCGSSREHAPWALLDY
                     GLRCVIAPSFGDIFYNNSFKNGLLPVVLQEAEVDRLFYECASFPGFRLTVDLDAQTVA
                     TADRGQVMRFEVDAFRKYCLLNGLDEIGLTLRHADKIRTFEAQHLRRYPWLAR"
BASE COUNT          338 a          718 c          749 g          278 t
ORIGIN      
        1 aggacgacgg cactgcgctg atctacatcg accggcacct cgtgcacgag gtcaccagcc
       61 cgcaggcgtt cgagggcctc aagctggcgg gccgccagcc gtggcgcatc gagtcggtgg
      121 tcgcgacggc cgaccacaac acgccgacca ccggctggga gcgcggcatc gaggggatca
      181 gcgacccgac ctcgcgcctc cagatcgaca cgctcgacgc caacatgcgc gagtacggca
      241 agcgggcgaa ggcctacttc ccgttcctcg accggcgcca gggcatcgtc cacgtgatcg
      301 ggcccgagca gggcgcgacg ctgcccggca tgaccgtcgt atgcggcgac tcgcacacca
      361 gcacgcacgg cgcgttcgcg tgcctcgcgc acggcatcgg cacctccgag gtcgagcacg
      421 tgctcgcgac gcagtgcctg gtcacgcaga aaatgcggaa catgcgcgtc agcgtcgagg
      481 gcgcgctgcc gcgcggcgtc ggcgcgaagg acgtcgtgct cgcgatcatc ggccggatcg
      541 gcacggcggg cggcagcggc tacgcgatcg agttcgcggg cagcgcgatc cgcgcgctgt
      601 cgatggaagg gcgcatgacc gtgtgcaaca tggcgatcga ggcgggcgcg cgcgccggca
      661 tggtcgcggt cgacgacacg acgctcgcct acctgaaggg ccggccgttc gcgccggcgg
      721 gccccgactg ggatgccgcc gtcgcctact ggaagacgct caagtccgac cccgacgcga
      781 agttcgaccg cgaagtcgcg ctgcgcgccg aggagatcgt gccgcaggtg tcgtggggca
      841 cctcgcccga gatggtcgtg ccggtcgacg gggaggtgcc cgatccggag cgcgagaagg
      901 actcgatccg gcgcgagggc atggagcgcg cgctgcagta catggggctc aagccgcgca
      961 cgcggatcac cgacatccgg atcgacaagg tgttcatcgg ctcgtgcacc aactcgcgca
     1021 tcgaggacct gcgcgtcgcc gcggcggtcg tgaagggccg gcgcgtcgcc gccaacgtgc
     1081 ggctcgcgat ggtcgtgccg ggctcggggc tcgtcaaggc gcaggccgag aaggaagggc
     1141 tcgaccgggt gttccgggac gccggcttcg actggcgcca gccgggctgc tcgatgtgcc
     1201 tcggcatgaa cgccgaccgg ctcgacccgg gcgagcgctg cgcgtcgacc tcgaaccgca
     1261 acttcgaggg ccgccagggc gacggcgggc gcacccacct ggtcagcccg gcgatggcgg
     1321 cggccgccgc ggtggccggg cacttcgtcg acgtccggca gtggcgctga gggcgggatg
     1381 gaaaagttca ccgtgcacac cgggatcgtc gcgccgctcg accgcggcaa cgtcgacacc
     1441 gaccagatca tcccgaagca gttcctgaag tcgatcaaga agaccggcta cgggccgaac
     1501 ctgttcgacg cctggcgcta catggaccac ggcgagccgg gcatggacca cgcgcggcgg
     1561 ccgcgcaacc cgaacttcgt gctcaacgac ccgcgctaca aggacgcgac gatcctgctg
     1621 gcgcgcaaga acttcggctg cggcagctcg cgcgagcacg cgccgtgggc gttgctcgac
     1681 tacggcttgc gctgcgtgat cgcgccgtcg ttcggcgaca tcttctacaa caacagcttc
     1741 aagaacggcc tgctgccggt ggtgctgcag gaggccgagg tcgaccggct gttctacgag
     1801 tgcgcgtcgt tcccgggctt ccggctgacg gtcgacctcg acgcgcagac cgtggcgacc
     1861 gccgaccgtg gccaggtcat gcgcttcgag gtcgacgcgt tccgcaagta ctgcctgctc
     1921 aacggcctcg acgagatcgg cctgacgctg cgccacgccg acaagatccg cacgttcgag
     1981 gcacagcacc tgcggcgcta tccctggctg gcgcggtgat ccgatgaaga tcgccgtgct
     2041 gcccggcgac gggatcggaa cagagatcgt cgccgaggcg gtg
//
LOCUS       DASPFR010000377         3066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1333052, whole genome shotgun sequence.
ACCESSION   DASPFR010000377 DASPFR010000000
VERSION     DASPFR010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333052"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VD737_09965"
     CDS             complement(<1..418)
                     /locus_tag="VD737_09965"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HXW10933.1"
                     /translation="MPRRHSQDIQRYSTARLLTATATTLLLAACGGGSGDGSGDGSPA
                     LSTGSVAVLFTDAPTDDFCQVRATVTGIDLLSDGGAATNLYTGERTVDVLAMRNFSDF
                     FTVDPAVPIGTYSKVRLTLADLALVECDAGGVPEPES"
     gene            complement(675..872)
                     /locus_tag="VD737_09970"
     CDS             complement(675..872)
                     /locus_tag="VD737_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10934.1"
                     /translation="MSIKFYAHFLVEQPQPRGLEEFRGVVELDGQPRPGRGTREAERI
                     IARNFGRSAQDIKVLQWARLH"
     gene            957..1469
                     /gene="greB"
                     /locus_tag="VD737_09975"
     CDS             957..1469
                     /gene="greB"
                     /locus_tag="VD737_09975"
                     /inference="COORDINATES: protein motif:HMM:NF002506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HXW10935.1"
                     /translation="MTRYRTPQAPGSRYSTPEGARRLRDELDQLWRVQRPQVTRAVQE
                     AAAQGDRSENAEYTYGKRQLREIDRRVRFLRKRLEGMVVVSRPPDDRSRVFFGAWVTV
                     EDECGERQSLRIVGPDELDPARRYVSMDAPLARALMGKRVDDEVTIDVPGGRKGYVVV
                     EIRYEEVASG"
     gene            complement(1552..1989)
                     /locus_tag="VD737_09980"
     CDS             complement(1552..1989)
                     /locus_tag="VD737_09980"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXW10936.1"
                     /translation="MGSAAEGSWAEGGCLCGAIRYRIPLPPLWVAHCHCSMCRRAQGA
                     AFVTWLGVPGERFSFLGTADRLRMFRSSADAQRSFCGRCGTPLFFESKHWPGELHVTL
                     ASLDPAVAATLQPQMHAYWSSRAPWVELGDHLPRKDPADHGSG"
     gene            complement(2034..>3066)
                     /locus_tag="VD737_09985"
     CDS             complement(2034..>3066)
                     /locus_tag="VD737_09985"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HXW10937.1"
                     /translation="RTNYALREEALALRDNKHRPTAVLTHGANPGLVSHFVKQALINI
                     ANDTGVEHKDPGTRAEWAALAQQLGVKTIHIAERDTQVAAGHPKQMNEFVNTWSVDGF
                     VSEGCQPAELGWGSHEKNWPRDGRKHDYGRGSAIYLMQPGAATRVRTWTPAAGHFHGF
                     LITHGESISISDYLTVKKGEQLVYRPTVHYAYHPSDNAVLSVHELAGRNWELQERKRI
                     MMEDITSGIDELGVLLAGHKKNAYWYGSQLSIDEARQLAPHNSATSLQVTVSALAGLV
                     WAIENPNAGIVEPDEIDHKRILEICRPYLGPVVGEYSEWHPLVDRGRLFPEDLDETDP
                     WQFKNVRVV"
BASE COUNT          495 a         1010 c         1064 g          497 t
ORIGIN      
        1 cggactcggg ctcgggcacg cccccggcat cgcactcgac gagcgcgagg tccgcgagcg
       61 tcaggcgcac cttcgaatac gtgccgatcg gcacggccgg atcgaccgtg aagaaatcgg
      121 agaagttgcg catcgcgagc acgtcgacgg tgcgctcacc cgtgtacagg ttggtggcag
      181 cacctccgtc ggacagcagg tcgatgcccg tgacggtcgc gcgcacctgg cagaagtcgt
      241 cggtcggtgc gtcggtgaag agcaccgcca cgctgccggt cgacagggcc ggcgagccgt
      301 cgcctgaacc gtccccggag ccgccgccgc acgccgcgag cagcagcgtc gtcgcggtcg
      361 cagtgagtag cctggcggtc gaataccgct gaatgtcctg tgaatgtctg cgcggcatga
      421 agcctcctga caggtctgcg tctccgggta gtgaaatcat tgtgcgccga agcgagccgt
      481 gaacaggtgc ggatctgtcg gcgagtggcg acaagcgcgc gcgacgccgc gatatgtgaa
      541 agcgatatgt caaatggtcg tgccgaggct gcatggacac gaaaaaaagc cccgggcttt
      601 tggcccgggg cgagtgagcg gactttggtc gaagggaagg gaaaagtccg ccaggggaac
      661 cgagatcgtg gacgtcagtg caggcgcgcc cactgcagga ccttgatgtc ctgcgccgag
      721 cgtccgaaat tgcgagcgat gatccgctcc gcttcccggg tgccacggcc cgggcgcggc
      781 tgcccgtcga gctcgaccac gccgcggaat tcctcgagcc cgcgcggttg cggctgctcg
      841 acgaggaagt gcgcgtagaa cttgatgctc atggcacgca aactaatggc ccccaccggc
      901 gttttccgtg acctcgcgca cacaatcccg aagcgcaacc ggccgatcga cgaaccatga
      961 cccgctaccg cactcctcag gcccccggct cgcgttattc gactcccgaa ggcgcccggc
     1021 ggctgcgcga cgagctcgac cagctctggc gcgtgcagcg tccgcaggtg acgcgcgcgg
     1081 tgcaggaagc cgccgcgcag ggcgaccgct cggagaacgc cgagtacacg tacggcaagc
     1141 ggcagctgcg cgagatcgac cgccgcgtcc ggttcctgcg caagcgactc gaaggcatgg
     1201 tggtcgtgag ccgtccgccc gacgatcgca gccgggtctt cttcggcgcc tgggtcacgg
     1261 tcgaggacga atgcggcgag cggcagtcgc tgcggatcgt cggccccgac gaactcgatc
     1321 ccgcgcgtcg ctacgtcagc atggacgcgc cgctcgctcg cgccctgatg ggcaagcgcg
     1381 tcgacgacga ggtcacgatc gacgtgcccg ggggacggaa gggctatgtg gtggtggaga
     1441 tccgctacga ggaagtggct agtggctagt ggtcagtggt cagtgaggag aaccaaagag
     1501 gtcggcgcta ccgcaacccg ctcctcttca ctaaccactg gccactgacc actaaccact
     1561 tccgtgatcg gccgggtcct tccgcggcag gtgatcgccc agctcgaccc agggcgcgcg
     1621 actgctccag tacgcgtgca tctgtggctg cagcgtcgcc gcgacggcgg gatcgaggct
     1681 ggcgagcgtc acgtgcagtt cgccgggcca gtgcttcgac tcgaagaaca gcggcgtgcc
     1741 gcagcgcccg cagaagctgc gctgggcatc ggccgaggag cggaacatgc gcaggcgatc
     1801 cgcggtgccg aggaaagaga atcgctcgcc cggaacgccc agccacgtga cgaacgccgc
     1861 gccctgggca cggcggcaca tcgagcagtg gcagtgcgcg acccacagcg gcggcaacgg
     1921 gatgcgatag cggatcgcgc cgcagaggca gccgccctcg gcccagctgc cctcggccgc
     1981 gctgcccacg ctttctcccg cggatgcggg agaggggttg gaattcggag gactcacacc
     2041 acccgcacgt tcttgaactg ccacgggtcg gtttcgtcga ggtcctcggg gaacagcctc
     2101 ccgcggtcca ccagcggatg ccactcgctg tactcgccga ccacggggcc gaggtacggc
     2161 cggcagattt cgaggatgcg cttgtggtcg atttcgtcgg gctcgacgat gccggcgttc
     2221 gggttctcga tcgcccagac caggcccgcg agggcggaca ccgtcacctg cagcgacgtg
     2281 gcgctgttgt gcggcgcgag ttggcgcgcc tcgtcgatcg acagctgcga gccgtaccag
     2341 taggcattct tcttgtggcc cgccagcagc acgccgagct cgtcgatgcc gctggtgatg
     2401 tcctccatca tgatccgctt gcgctcctgg agctcccagt tgcgtcccgc gagttcgtgg
     2461 acggagagca cggcgttgtc gctcgggtgg taggcgtagt gcaccgtcgg acggtagacg
     2521 agctgctcgc ctttcttgac cgtgaggtag tcggagatcg agatcgactc gccgtgcgtg
     2581 atcaggaagc cgtggaaatg gcccgcggcc ggcgtccacg tgcgcacgcg cgtggccgcg
     2641 cccggttgca tcaggtagat cgcgctgcca cggccgtagt cgtgcttgcg gccgtcgcgt
     2701 ggccagttct tctcgtgcga accccagccg agctcggccg gctggcagcc ttcgctcacg
     2761 aagccgtcca ccgaccatgt gttgacgaac tcgttcatct gcttcggatg gcccgccgcg
     2821 acctgcgtat cgcgctcggc gatgtgtatc gtcttgacgc cgagctgttg tgcgagcgcg
     2881 gcccattcgg cgcgcgtgcc cggatccttg tgctcgacac cggtgtcgtt cgcgatgttg
     2941 atcaacgcct gcttgacgaa gtgcgacacc aggcccgggt tggcgccgtg agtcagcacc
     3001 gccgtcggcc ggtgcttgtt gtcgcgcagc gcgagcgctt cctcgcgcag cgcgtagttc
     3061 gtgcgc
//
LOCUS       DASPFR010000378         5598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1210418, whole genome shotgun sequence.
ACCESSION   DASPFR010000378 DASPFR010000000
VERSION     DASPFR010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210418"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="VD737_09990"
     CDS             complement(<1..825)
                     /locus_tag="VD737_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HXW10938.1"
                     /translation="MRDSFKTRTSLAVGHQSYEIHSLPALQGHDLDRLPYSLKVLLEN
                     LLRFEDGVNVTREDIEALLAWNAKAAPSYEIAFTPARVIMQDFTGVPAIVDLAAMREA
                     MVRLGGNPQEINPLAPAELVIDHSVQVDAYGAPDSATRNTTIEFERNGERYSFLRWGQ
                     NAFRNFNVVPPSTGIVHQVNIEHLARVVFREVVNGRARAYPDTLVGTDSHTTMVNGLG
                     VLGWGVGGIEAEAAMLGQPMSMLIPPVVGFKLTGAMPEGATATDLVLMVTERLRKHG"
     gene            complement(865..1977)
                     /gene="mnmA"
                     /locus_tag="VD737_09995"
     CDS             complement(865..1977)
                     /gene="mnmA"
                     /locus_tag="VD737_09995"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004646533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HXW10939.1"
                     /translation="MRVVLALSGGVDSAVSAVLLRQAGHDVHALFMRNWDEDEDGYCT
                     AAADLQDARKVCEDLGIPLHTASFAAEYRERVFEHFLAELRAGRTPNPDVACNREIKF
                     GVCFEHATRLGAEMFATGHYARSEGGRLLRGIDAGKDQSYFLHTVPGAMLARTLFPVG
                     ALPKREVRSIAHEHALPVHDKRDSTGICFVGERPFAEFLRTYMPAQPGAIETPEGRRL
                     GSHRGLMYYTLGQRQGLAVGGVRGAAERPWYVAGKDLARNALIVVQQPDHPLLLSDTI
                     TTEPAQWISGEPPVAKGERLRCTVKTRYRQADQACEVEILADGRCTVRTDVAQRAVTP
                     GQSAVFYADDVCLGGAIIAHAELRGSRVESGRAVGL"
     gene            complement(2110..2592)
                     /locus_tag="VD737_10000"
     CDS             complement(2110..2592)
                     /locus_tag="VD737_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXW10940.1"
                     /translation="MVWKPDVTVAAVVERDGKFLFVEERAGGRVVLNQPAGHLENGET
                     LIDAVARETLEETGWTFVPQAVVGLYVWQPEHLSRTFLRVAFGGRLDGHDPSRPLDHG
                     ILRTRWLDREQLLASGPKHRSPLVLRCVDDYLGGARYPLELLTHLMRGASAASRVSSG
                     "
     gene            complement(2681..3187)
                     /locus_tag="VD737_10005"
     CDS             complement(2681..3187)
                     /locus_tag="VD737_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10941.1"
                     /translation="MEQPVDTTGTRSLLANLDEYRIAVAEIATRARRSLSIYTPDLEP
                     QIYDTDLFLDPVKRLVLARSHARLRVLISNPGRVSRDGNRFMMMARRLTSYIDLRNVA
                     PEYRNNPCSFIVADDKAIVYRQQATKWEGLVEFDDVSIVKRYLAYFDEVWAGSLIQPE
                     MRATAVDF"
     gene            complement(3132..3551)
                     /locus_tag="VD737_10010"
     CDS             complement(3132..3551)
                     /locus_tag="VD737_10010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXW10942.1"
                     /translation="MEQRIPEHEEWDDLDPVVPHVLAWSPAAEAKRDLVGTGRLEPTG
                     KIGRVAVLPQYRGRGAGLAIMHRLVELARERGFAEVHLNAQAYARGFYERLGFRAEGP
                     EFDEVGIPHQRMRRAVGNSDGTTGGHDGDAQPAREPR"
     gene            complement(3714..5081)
                     /gene="purB"
                     /locus_tag="VD737_10015"
     CDS             complement(3714..5081)
                     /gene="purB"
                     /locus_tag="VD737_10015"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF006764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HXW10943.1"
                     /translation="MSLTPLTALSPVDGRYAARCADLRGIFSEQGLIRARVRVEVAWL
                     HALAADRHIQELRGLTADDLAVAQRVAEAFDAADAAEVKAIERETNHDVKAVEYLIKK
                     KLSAHPSWASRVEFVHFACTSEDINNLSYALMCREARDNVLLPHLDVLLGALRSMAHA
                     HADDAMMSRTHGQAATPTTLGKEVAVFVHRLRLARSTFAAVPIRGKINGAVGNFNAHL
                     AAYPNVDWPRLAQRLVESLKLEWNPYTTQIEPHDWNAEYCDALARCNSILLDLCRDFW
                     GYVSLGYFRQKVVAGEVGSSTMPHKVNPIDFENAEGNLGLANALLRFLSEKLPVSRWQ
                     RDLTDSTVQRNIGVALAHADIAVQSVSRGLARLEVDRVALADDLDRNWELLAEPIQTV
                     MRRYAVPEAYEQLKALTRGRRVDAESVARFVDGLPIPPAEKERLRKLSPAAYVGLAAK
                     LARDV"
     gene            5171..>5598
                     /locus_tag="VD737_10020"
     CDS             5171..>5598
                     /locus_tag="VD737_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HXW10944.1"
                     /translation="MTDTDYRVERDSMGETRVPSRALWGAQTQRAVQNFPISGLTMPR
                     GFIRALGLVKWAAAGANLELGDLDRTRAAAIQRASLEVAEGRHDSHFPIDVFQTGSGT
                     SSNMNANEVVARLATDLAGGNTPVHPNDHVNRGQSSNDVIP"
BASE COUNT          900 a         1854 c         1947 g          897 t
ORIGIN      
        1 gccgtgcttg cgcagccgct cggtgaccat cagcaccaga tcggtcgccg tggcgccctc
       61 cggcatggcc ccggtcagct tgaacccgac gaccggcggg atgagcatcg acatcggctg
      121 acccagcatc gcggcctccg cctcgatgcc gccgacaccc cagccgagca cgccgaggcc
      181 gttcaccatt gtcgtgtggg aatcggtgcc gaccagcgtg tcggggtagg cgcgggctcg
      241 gccgttcacc acctcgcgga acacgacgcg cgcgaggtgc tcgatgttga cctggtgcac
      301 gatgccggtc gacggcggca cgacgttgaa gttgcggaag gcgttctggc cccagcgcag
      361 gaacgaatag cgctcgccat tgcgctcgaa ctcgatggtc gtgttgcggg tggcggaatc
      421 gggcgcgccg tacgcgtcga cctgcacgga gtggtcgatc acgagctcgg ccggtgcgag
      481 cgggttgatc tcctgggggt tgccgccgag gcgcaccatc gcctcgcgca tcgcggcgag
      541 gtcgacgatg gccggcacgc ccgtgaagtc ctgcatgatc acgcgggcgg gcgtgaaggc
      601 gatttcgtag ctgggcgctg ccttggcatt ccacgcgagc agcgcctcga tgtcctcgcg
      661 cgtcacgttg acgccgtctt cgaagcgcag caggttctcg agcagcacct tgagcgagta
      721 gggcaggcgg tcgaggtcgt ggccctgcag tgccgggagg ctgtggatct cgtaagactg
      781 gtgaccgacc gcaagcgagg tgcgggtctt gaagctgtct ctcatggaac ctccggcagg
      841 aaacggcgcg accaaaccgc ggaattatag accgacggcc cgtccggatt cgacccgaga
      901 accgcgaagc tccgcgtgcg cgatgattgc gccgccgaga catacgtcat cggcgtagaa
      961 gacggcggac tggcccggcg tgaccgcgcg ctgcgccacg tctgtgcgca ccgtgcagcg
     1021 accgtctgcc aggatctcga cctcgcaggc ctggtcggcc tgccgataac gcgtcttgac
     1081 ggtgcagcgc aggcgctcgc ccttcgcgac gggcggctcg cccgaaatcc actgcgccgg
     1141 ctcggtcgtg atcgtgtcgg agagcaacag cggatggtcg ggctgctgaa ccacgatcag
     1201 cgcgttgcga gcgaggtcct tgcccgcgac ataccagggc cgttcggccg cgccccgcac
     1261 gccgcccacc gcaagtccct gccgctgccc gagcgtgtag tacatcagcc cgcgatgcga
     1321 gcccaggcgc cggccctccg gcgtctcgat cgcgccgggt tgcgccggca tatacgtccg
     1381 caaaaactct gcgaacgggc gctcgccgac gaagcagatg ccggtgctgt cgcgcttgtc
     1441 gtgcacgggc agcgcgtgct cgtgcgcgat gctccgcacc tcgcgcttcg gcagcgcgcc
     1501 gaccggaaac agcgtgcgcg ccagcatggc gcccggcacg gtgtgcagga agtagctctg
     1561 gtccttgccg gcatcgatgc cgcgcagcag ccgaccgccc tccgagcgcg cgtagtggcc
     1621 ggtggcgaac atctccgcgc cgagacgcgt cgcgtgctcg aagcagacgc cgaacttgat
     1681 ctcgcggttg caggcgacgt cgggattggg ggtgcggccg gcgcgcagct cggcgaggaa
     1741 gtgctcgaac acgcgctcgc gatactccgc ggcgaacgac gccgtgtgca gcgggatgcc
     1801 gaggtcctcg cagaccttgc gggcgtcctg cagatcggcg gccgccgtgc agtacccgtc
     1861 ctcgtcctcg tcccagttgc gcatgaacag cgcgtgcacg tcgtggccgg cctgcctgag
     1921 caggaccgcg gagacggccg aatcgacacc gccggagagg gccaggacga cgcgcattcc
     1981 tggattctaa cggagggtgg cagtggtcag tggctagtgg ctagtgatta gtggctagtg
     2041 aggaagaacg cgttctttaa gtccgactag ccactagcca ctagccacta gccaccagca
     2101 cccgacccac tagccactcg agactcggct ggcggccgag gcgccccgca tcaggtgcgt
     2161 cagcagctcc agcgggtagc gggcaccgcc gagatagtcg tcgacgcagc ggagcacgag
     2221 cgggctgcgg tgcttggggc cggaggccag cagttgctcg cgatcgagcc aacgcgtgcg
     2281 caggatgccg tgatcgagcg gtcggctggg gtcgtggccg tcgaggcggc cgccgaacgc
     2341 aacgcgcagg aacgtgcggc tcaggtgctc gggctgccag acgtacagcc cgacgacggc
     2401 ctgcggcacg aacgtccagc ccgtctcctc gagcgtctcg cgggccaccg catcgatcag
     2461 cgtctcgccg ttctccaggt ggccggccgg ctggttcagc acgacccgtc caccggcacg
     2521 ctcttccacg aacaggaact tgccgtcgcg ctcgaccacc gcggcgacgg tcacgtcggg
     2581 tttccagacc atgtccgaca ttatagcgag ggtgctgcgg gcggaaccgg gcccgcggac
     2641 gggcaagcgc agcgcctacg gcgacacggg caatgccgcg tcagaagtcc accgccgtgg
     2701 cgcgcatctc cggctggatc aggctcccgg cccagacctc gtcgaaatag gcgaggtagc
     2761 gcttgacgat cgagacgtcg tcgaattcga cgaggccttc ccatttggtc gcctgctggc
     2821 ggtacacgat ggccttgtcg tcggcgacga tgaacgagca ggggttgttg cggtactccg
     2881 gcgcgacgtt gcgcaggtcg atgtagctcg tcagccggcg ggccatcatc atgaatcggt
     2941 tgccgtcgcg cgagacgcgc cccgggttcg agatcagcac gcgcagccgc gcgtggctgc
     3001 gcgccagcac gaggcgcttg acgggatcga ggaagaggtc ggtgtcgtag atctggggct
     3061 cgaggtcggg cgtgtagatg gacagcgagc gccgggcgcg cgtggcgatc tccgccacgg
     3121 cgatgcggta ctcatcgagg ttcgcgagca ggctgcgcgt ccccgtcgtg tccaccggtt
     3181 gttccatcgc tgttcccgac cgcacgtcgc atgcgctggt gcggtatccc gacctcgtcg
     3241 aactcggggc cctcggcccg gaaacccagc cgctcgtaaa atccgcgggc gtaagcctga
     3301 gcattcaggt gcacctcggc gaaaccacgc tcacgcgcga gttccacgag tcggtgcatg
     3361 atggcgagac cggctccgcg gccgcggtac tgcggcagta cggcgacgcg gccaatctta
     3421 ccggtgggct ccagacggcc cgtgccaacc aggtcacgtt ttgcctccgc tgcgggcgac
     3481 catgccagta cgtgcggtac tacggggtcg aggtcgtccc attcctcgtg ctcggggatg
     3541 cgctgttcca ctatgaaaac agccctgcgc acgctttcga tcgccgcacg gtcatgctgc
     3601 cacgatgcaa gctcgacctg gatgccggga cgcggagatg gcgtcatttc ggagacgcaa
     3661 gtttccgtta tgtcaagacg cggattcgct tatgtcaaaa ctgccgcccg ctgtcagacg
     3721 tcgcgcgcga gtttcgccgc cagcccgacg tacgcggcgg ggctgagctt gcgcaaccgc
     3781 tccttctcgg ccgggggaat cggcagcccg tcgacgaacc tcgcgaccga ttcggcgtcg
     3841 acgcgccggc cgcgcgtcag cgccttgagc tgctcgtagg cctccggcac ggcgtaacgc
     3901 cgcatgacgg tctggatcgg ttccgccagc agttcccagt tccgatcgag gtcatcggcg
     3961 agggcgacgc ggtcgacctc gagccgtgcc aggccgcgcg agacggactg gaccgcgatg
     4021 tcggcatgcg cgagcgccac tccgatgttg cgctgcacgg tcgagtcggt gaggtcgcgc
     4081 tgccaccgtg acacgggcag cttctccgac aggaaccgca gcaacgcgtt ggccagtccg
     4141 aggttgcctt cggcgttctc gaaatcgatc ggattgacct tgtggggcat cgtggacgaa
     4201 ccgacttctc cggcgacgac tttctggcgg aagtagccga gcgacacgta gccccagaaa
     4261 tcgcggcaga ggtcgagcag gatcgagttg cagcgcgcga gtgcatcgca gtattccgcg
     4321 ttccagtcgt gcggctcgat ctgggtcgtg tacgggttcc actcgagctt gagcgattcg
     4381 acgagccgct gcgcgaggcg gggccagtcg acgttgggat aggcggcgag gtgcgcgttg
     4441 aagttgccga cggcaccgtt gatcttgccg cggatcggca ctgccgcgaa cgtgctgcgc
     4501 gccaggcgca gccggtgcac gaagacggcg acttccttgc cgagcgtggt cggcgtcgcg
     4561 gcctggccgt gggtgcgcga catcatggcg tcgtcggcgt gcgcgtgcgc catcgagcgc
     4621 agcgccccga gcagcacgtc gaggtggggc agcagcacgt tgtcacgagc ctcgcgacac
     4681 atcagcgcgt acgagaggtt gttgatgtcc tcggacgtgc aggcgaagtg cacgaactcg
     4741 acccgcgagg cccaggacgg atgcgccgac agtttcttct tgatcaggta ctcgacggcc
     4801 ttgacgtcgt gattggtttc ccgctcgatc gccttgacct cggctgcgtc ggcggcgtcg
     4861 aaggcctcgg cgacgcgctg tgcaaccgcg agatcgtccg ccgtcaggcc gcgcagctcc
     4921 tggatgtggc gatcggccgc gagtgcgtgc agccaggcga cctcgacgcg gacccgcgca
     4981 cggatgagtc cctgctcgct gaagatgccc cgaagatcgg cgcagcgcgc ggcgtaccgt
     5041 ccgtccacgg gagagagcgc ggtcagcggc gtgagactca tgcggaaatc cgggaggtgc
     5101 tacgatcgac gatgataccg cagggcccgg ttccggcgcc cgcacgggct tgaaacttag
     5161 gtttttcccg atgaccgaca ccgactaccg cgtcgagcgc gacagcatgg gcgagacccg
     5221 ggtgccgtcg cgggcgctct ggggtgccca gacccagcgt gccgtgcaga attttccgat
     5281 cagcggattg accatgccac gcggcttcat ccgcgcgctg ggcctggtca agtgggcggc
     5341 ggccggtgcc aacctcgaac tcggcgacct ggatcgcacg cgcgctgctg cgatccagcg
     5401 agcgtcgctc gaggtggccg agggccggca tgattcgcat ttcccgatcg acgtcttcca
     5461 gaccggctcg ggcaccagca gcaacatgaa cgcgaacgaa gtcgtcgcgc ggcttgccac
     5521 cgaccttgcg ggcggcaaca cccccgtgca tccgaacgac cacgtcaacc gcggccagag
     5581 cagcaacgac gtgatccc
//
LOCUS       DASPFR010000379         4675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_965513, whole genome shotgun sequence.
ACCESSION   DASPFR010000379 DASPFR010000000
VERSION     DASPFR010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965513"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VD737_10025"
     CDS             <1..524
                     /locus_tag="VD737_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10945.1"
                     /translation="HVLPLEAAAPIAVGDGATLLPVAAFETRGRVLNIERFKPHQSLV
                     NWIPGLRPATHDIGLGYGPMTDSANVGRFSFSHEGVSHGLRALFARPRDAMTQQEFDA
                     LAPHITNVHVIPASATVYAQLRRVRHGELVTLRGLLVNVRDAEGRVATTSTTPGDRDC
                     EILWLEELRIDRL"
     gene            533..700
                     /locus_tag="VD737_10030"
     CDS             533..700
                     /locus_tag="VD737_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10946.1"
                     /translation="MTLFEWLAQVCSELGWPVMRAHEMLDETRWSDMYEAGVSPAEAA
                     ARTRAETDATS"
     gene            803..3418
                     /locus_tag="VD737_10035"
     CDS             803..3418
                     /locus_tag="VD737_10035"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HXW10947.1"
                     /translation="MFSVVDRPPVPLTDPAAAWHASPAQQALERLGSGPRGLTAAEAA
                     ARLARDGPNALAEGASRGPLRIFFAQFADFMIGVLLVAAGVAFVIGEAIEAGAILAIV
                     VLNAVLGFVQEWRAEQAIAALRSLAAPHAHVLRAGVRTELPAADLVVGDVVWLEAGAR
                     VPADLRLIDSAALRIDEAALTGESVPVDKEAQLTFEPDTVLADRQNMAWSGTSVTGGR
                     AHGLVVAIGRATQLGNIARLVEQAGETRTPLQDRLARFGRRLALAVLAICAVIFAAGL
                     ARGEPAALMFLTAISLAVAAIPEALPAVVSIALAFGARRMSRANALVRRLPCVESLGS
                     VTVICTDKTGTLTENRLELARFEVTRDATRDHTLEVLALNSDAARAEAGHWLGDPTEI
                     ALVDHAHASGIDVEALREALPRIAELPFDSERKRMTTVHTQDDRVLVTVKGAPEAVLP
                     ICVGLDTAEWHRRADAMAADGLRVLAIAARGADPHLPIEDLERDLHLLALVGLIDPPR
                     PEAQAAVAECLAAGIRPVMITGDHPSTALAIARALGIAEDAASVVTGAELERMDDTTL
                     AARLADARVFARVDPAQKIRIVRALQARNESVAMTGDGVNDAPALKQSDVGVSMGRGG
                     TDVARDASDLVLLDDNFATIVDAVREGRRIYDNIRKFVRFVMAGNAGEIWTIFLAPFL
                     GLPIPLTPIQILWVNFITDGLPGLALTSEPAERHVMRRRPRPRSESVFAHGIWQHILW
                     AGLLIGIVSLAACAWAIAHGEHPQTMAFTVLTFAQLAHVMAIRSETDSLWRLGLLSNR
                     FMLLTVAASVALHLAIVYLPLLQPVFGTEPLSARAMLVATALSLAVLLGVEIEKWAAR
                     RGWLYDLRTTEPRVS"
     gene            3483..4613
                     /locus_tag="VD737_10040"
     CDS             3483..4613
                     /locus_tag="VD737_10040"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HXW10948.1"
                     /translation="MNAPDAISRRSNLDAALLARTAPLPIASYFDPAWFEREMLLLFD
                     KGPNYVGSEMMVPEVGDYMTLPWTDNGKVLVHGPNGVNLLSNTCRHRQGLILEGRGHG
                     DNLVCPLHRWTYDLDGRLLGAPDFDVTPDCALPKTALRRWNGLLFAGPRDPSADLADF
                     PLAADYDYRNYVFDKMTVDECPFNWKAFLEIFLELYHVEAMHPGLQKWVDAGNYEWGF
                     GDRWSYQTLGIKDGLQSQVSPNYQKYRDAILAYTGGQLPKYGTVWSILYPNVMLEWYP
                     QALVVSTLIPRSPDRTTNIVEFYYPEEVQAFERQIVEAHQAAYAESAAEDAEICARLQ
                     RGRRALWLAGEDDEGPYHTPHEDGMIHMHDWIRRELRQRRTL"
BASE COUNT          698 a         1656 c         1610 g          711 t
ORIGIN      
        1 atcacgtgct gccgctcgag gccgcagcgc caatcgccgt tggcgacggc gcaacgctgc
       61 tgcccgtggc cgcgttcgag acgcgtggac gcgtgctgaa catcgagcgc ttcaagccgc
      121 atcagtcgct ggtcaactgg attcccggcc tgcgtcccgc cacccacgat atcggactcg
      181 gctacggacc gatgacggac agcgccaatg tcgggcgctt ttcgttctcg catgaaggcg
      241 tcagccatgg actgcgcgcg ctgtttgctc gtccgcgcga tgccatgacg cagcaggagt
      301 tcgacgcgct ggcgccgcac atcaccaacg tgcacgtcat tccggcgagc gcgacggtgt
      361 atgcgcagct gcggcgcgtg cggcacggtg agctcgtcac gctgcgcggc ctgctcgtca
      421 acgtgcggga cgccgagggg cgtgtcgcga cgacgtcgac cacgccgggt gaccgcgact
      481 gcgagatcct gtggctggag gaactccgga tcgatcgcct gtaggagcac ggatgacact
      541 gttcgaatgg ctggcgcagg tgtgcagcga actcggatgg ccggtgatgc gggcgcacga
      601 gatgctcgac gaaacccgct ggtccgacat gtacgaagcc ggcgtcagcc cggccgaggc
      661 agccgcccgc acacgggccg agaccgacgc cacctcgtaa accccgaacg ggtgccggcg
      721 ccgccgttga cgggcggcaa gcggctgcgc cggccggcgc ctagattgaa gcgatccggc
      781 ttgccgtcgc gagcccgcgt tcgtgttttc cgtcgtggac cgcccgcccg ttcccctcac
      841 cgatcccgcg gccgcgtggc acgcctcgcc cgcgcaacag gcgctcgagc gcctgggctc
      901 cggtccacgc ggcctcaccg ccgccgaggc tgccgcccgg ctggcgcgcg atgggccgaa
      961 cgcgctcgcc gagggcgcct cgcgcgggcc gctgcgcatc ttcttcgcgc agttcgcgga
     1021 cttcatgatc ggcgtgctgc tggtcgccgc tggcgtggct ttcgtgatcg gcgaggcgat
     1081 cgaggccggc gcgatcctcg cgatcgtcgt gctgaacgca gtgctcgggt tcgtccagga
     1141 gtggcgtgcg gagcaggcga ttgccgcgct gcgttcgctc gcggcgccgc acgcgcacgt
     1201 gctccgagcg ggcgtgcgca ccgagcttcc ggcggccgac ctcgtcgtcg gcgacgtcgt
     1261 gtggctcgag gcgggggcac gcgtgccggc cgatctgcgg ctgatcgatt cggcggcgct
     1321 gcgcatcgac gaggcggcgc tgacaggcga atcggtgccc gtcgacaagg aggcgcagct
     1381 caccttcgaa cccgacaccg tgctggccga ccgccagaac atggcgtggt cgggcaccag
     1441 cgtgacaggc ggccgcgcac acggcctggt ggtcgccatc ggccgcgcca cgcagctggg
     1501 caacatcgcg cggctggtcg agcaggcggg cgagacacgc acgccgctgc aggaccggct
     1561 ggcgcgcttc ggccggcggc tcgcgctcgc ggtcctcgcg atctgcgccg tcatcttcgc
     1621 cgccggcctg gcgcgcggcg aaccggccgc gctgatgttc ctgaccgcga tcagcctcgc
     1681 ggtggccgcc attcccgaag cactgcccgc cgtcgtgtcg atcgcgctcg cgttcggcgc
     1741 ccgccgcatg tcgcgtgcga acgcgctggt gcggcggctg ccttgcgtcg agtcgctcgg
     1801 ttcggtcacg gtgatctgca ccgacaagac cggcacgctg accgagaacc ggctcgagct
     1861 ggcccgcttc gaggtcacgc gcgacgccac gcgcgaccac acgctcgagg tgctggcgct
     1921 gaacagcgac gccgcgcgcg cggaggcagg ccactggctg ggcgacccga ccgagatcgc
     1981 cctcgtcgat cacgcgcacg ccagcggcat cgacgtcgag gcgctgcgcg aggcgctgcc
     2041 gcggatcgcc gagttgccat tcgactccga gcgcaagcgc atgacgaccg tgcatacgca
     2101 ggacgaccgc gtgctcgtaa cggtgaaagg cgcgccggaa gcggtgctgc cgatctgcgt
     2161 cgggctcgac accgccgagt ggcaccggcg cgcggacgcg atggcggccg acggcctgcg
     2221 cgtgctcgcg atcgcagccc gcggcgccga cccgcacctg ccgatcgagg acctcgagcg
     2281 cgatctgcac ctgctcgcgc tggtcgggct gatcgacccg ccgcggccgg aagcgcaggc
     2341 ggcggtcgcc gagtgcctcg ccgcgggcat ccggccagtg atgatcaccg gagaccatcc
     2401 gtcgaccgcg ctcgccatcg cgcgtgcgct cggcatcgcc gaggacgccg ccagcgtcgt
     2461 cacgggcgcc gaactggagc ggatggacga caccacgctt gccgcgcggc tggccgacgc
     2521 acgggtgttc gcacgcgtcg acccggcgca gaagatccgt atcgtgcgcg cgttgcaggc
     2581 gcgaaacgaa agcgtggcga tgaccggcga cggcgtcaac gacgcgcccg cgctgaagca
     2641 gagcgacgtc ggcgtgtcca tggggcgcgg cggcaccgac gttgcgcgcg acgcttccga
     2701 cctcgtgctg ctcgacgaca atttcgcgac catcgtcgac gcggtgcgcg aaggccgccg
     2761 gatctacgac aacatccgca agtttgtgcg cttcgttatg gcgggcaacg cgggcgagat
     2821 ctggacgatc ttcctcgcgc cgttcctcgg cctcccgatc cctctgaccc cgatccagat
     2881 cctgtgggtc aacttcatca ccgacggcct gccgggactc gcgctgacga gcgagcccgc
     2941 ggagcgccac gtgatgcggc ggcggccgcg gccgcgctcg gagtcggtct tcgcgcacgg
     3001 aatctggcag cacatcctgt gggccgggct actgatcggc atcgtcagcc tcgctgcctg
     3061 cgcctgggcg atcgcgcacg gtgaacaccc gcagacgatg gccttcaccg tgctgacgtt
     3121 cgcgcagctc gcgcacgtga tggcgatccg ctccgaaacg gacagcctgt ggcggctcgg
     3181 cctgctgtcg aaccgcttca tgctcctcac cgtcgccgcg agcgtcgcgc tgcacctcgc
     3241 catcgtgtac ctgccgctac tgcagccggt gttcggaacg gagccgctgt cggcacgcgc
     3301 gatgctggtc gccaccgcgc tgtcgctcgc agtgctgcta ggggtcgaga tcgagaagtg
     3361 ggcggcgcgg cgcggctggc tgtacgacct gcgcacaacc gagccgcgcg tatcatgaat
     3421 cgcgccgacg cccgcgccgt cgggccgcgc gggcgcacct cctgcactcc cgaaccttcg
     3481 tcatgaacgc tcccgacgcc atctcccgcc gctccaacct cgacgccgcg ctgctcgccc
     3541 gtaccgcgcc gctgccgatc gcgagctact tcgatcccgc atggttcgag cgcgagatgc
     3601 tgctgctgtt cgacaagggg ccgaactatg tcggcagcga gatgatggtg ccggaagtcg
     3661 gcgactacat gacgctgccg tggactgata acggcaaggt tctggtgcac gggccgaacg
     3721 gcgtcaacct gctgtccaac acgtgccgcc acaggcaagg gctgatcctc gagggtcgcg
     3781 gccacggtga caatctcgtc tgtccgctgc atcgctggac gtacgacctc gacggcaggc
     3841 tgctcggcgc gcccgacttt gatgtcacgc ccgactgcgc gctgccgaag acggcgctta
     3901 ggcgctggaa tgggctgctg ttcgcgggcc cgcgcgaccc gtccgcggac ctcgccgact
     3961 ttccgctcgc cgccgactac gactaccgga actacgtgtt cgacaagatg accgtcgacg
     4021 agtgtccttt caactggaag gcattcctcg agatcttcct cgagctgtat cacgtcgagg
     4081 cgatgcatcc cggcctgcag aagtgggtcg acgccggcaa ctacgaatgg ggcttcggcg
     4141 accgctggag ctaccagact ctcggcatca aggacggcct gcagtcgcag gtgtcgccca
     4201 actaccagaa gtatcgcgac gcgatcctcg cctacaccgg cggtcaactg ccgaagtacg
     4261 gcaccgtgtg gtcgatcctg tatccgaacg tcatgctcga gtggtatccg caggcgctgg
     4321 tggtcagcac gctgatcccg cgctcgccag atcgcacgac gaacatcgtc gagttctact
     4381 accccgagga agtgcaggcc ttcgagcgcc agatcgtcga agcgcaccag gcagcgtatg
     4441 cggaatcggc ggcggaagac gccgagatct gtgcccgact acagcgcggc cgccgcgcgc
     4501 tatggctcgc cggcgaggac gacgagggtc cgtaccacac gccgcacgag gacggcatga
     4561 tccacatgca cgactggatc cgccgcgagc tacggcagcg gcgcacgctc tgagcgagcg
     4621 ttgacgcttg cagcgcggct tgcgagcagc aggcgtctgc gcgctgcccg atcct
//
LOCUS       DASPFR010000380         9943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1333180, whole genome shotgun sequence.
ACCESSION   DASPFR010000380 DASPFR010000000
VERSION     DASPFR010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9943
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333180"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /gene="queG"
                     /locus_tag="VD737_10045"
     CDS             complement(<1..1009)
                     /gene="queG"
                     /locus_tag="VD737_10045"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HXW10949.1"
                     /translation="MDVTDYDALARSIKDWGRELGFQKVGIAGVELPVDERRLEAWLA
                     LGRQGDMHYMGRHGTRRTRPAELVPGTVRVVSARMDYRPPGADPVTVLTDGKLGYVSR
                     YALGRDYHRLVRARLARLAGQIAEAANAAGYRAFTDSAPVLEKALARNAGLGWIGKHT
                     NLLDRHDGSWFFLGEVFTDLPLPPDAPVTSHCGSCTACIDVCPTQAIVAPYELDARRC
                     ISYLTIEHHGSIPPEFRVAIGNRIYGCDDCQLVCPWNRYAKLSAEPDFAARHGLDSPR
                     LTELFRWSEEDFLRCTEGSAIRRVGYERWLRNVAVALGNAPPSADVIEALRSREHDPS
                     EL"
     gene            1075..2553
                     /locus_tag="VD737_10050"
     CDS             1075..2553
                     /locus_tag="VD737_10050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HXW10950.1"
                     /translation="MQPIPAGLYSAAQVRELDRCAIERHGVPGYELMTRAGHAALDAL
                     RRAWPQARSVCVLCGPGNNGGDGYVLARLARAQNLRTHVFPLTDPARLHGDARRAYED
                     FAAAGGACEEWHASLLQQVDVVVDAVFGTGLARAVEGRVAHVFGSVNAAGRPVLALDV
                     PSGLDADTGHALGVAIRADVTVSFIGRKVGCYVGIGPDIVGRLVFDDLGVPPAVYEAV
                     SAKRHLLDESTVHAALPRRSRTAHKGRHGHVLIVGAAAGMGGAVRLAGEAALRVGAGL
                     VTAAVHPQSLVALTGRPELMTAAIADCAELERALERASIVAVGPGLGQSPWSMEVFGV
                     VLGCGKPLVVDADALNLLALDPQARESWVLTPHPGEAARLLRASTEQVQADRLGAAVE
                     LQARFGGTVVLKGAGSIVQGAGGTSWICDRGNPGMAAAGMGDVLTGVIAGIAGQCDDL
                     ALAARAGVLVHAMAGDRAALPGERGTTAGDVIAELRACVNPR"
     gene            2550..3026
                     /gene="tsaE"
                     /locus_tag="VD737_10055"
     CDS             2550..3026
                     /gene="tsaE"
                     /locus_tag="VD737_10055"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HXW10951.1"
                     /translation="MSLQFIAAGADGTRELGARLAVAWQASEAAARVATLVTLSGDLG
                     AGKTTFAAGLLQALGHAGPVRSPTYTLIEPYRFGARDVHHCDLYRLRHPQELDDLGLR
                     DLRAAGSLLLVEWPEKAEGRLGAPDLAVTLRYAGDAARELSVDARSEVGRDVLVHL"
     gene            3100..4461
                     /locus_tag="VD737_10060"
     CDS             3100..4461
                     /locus_tag="VD737_10060"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXW10952.1"
                     /translation="MRAREPRLIVVLVAWLGLSLAAMSASAATSRVKDVRLWSGPEGT
                     RLVIELTAPVEYDVFPLGNPNRVVIDLANTSLAGQELPAGQGPVQRVRSGPQPGKGLR
                     FVLDVESPQPPRHFAVRPDGTAGHRIVVELPGKSGPVVAATAVEPGTSGAGAPTGNEV
                     LAMVAETSRASAAPIVETPRVSDAPATPAVKSLASTAKGRDLVIAIDAGHGGQDPGAL
                     GRGGTREKEVTLAIARRLVAEINREEGMRAVLIRDGDYFVTLGGRTRKARKLGADMFI
                     SIHADSVLKRDVSGSSVYVLSLRGASDEAARWLAESENAADLRGGVPLVDDDLLASVL
                     LDVTQKEAISDSVEAADNVLAALASVGAVHGARRVRHAGFVVLKSPEIPSMLVETGFI
                     SNAGDERRLRDGSHQQKIAAAIHAGIRSYWYDNPPPGTRLAALAAARRGGSAGAPQTV
                     AVK"
     gene            4654..6501
                     /gene="mutL"
                     /locus_tag="VD737_10065"
     CDS             4654..6501
                     /gene="mutL"
                     /locus_tag="VD737_10065"
                     /inference="COORDINATES: protein motif:HMM:NF000949.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HXW10953.1"
                     /translation="MPIRVLPEQLVHQIAAGEVVERPASVIKELVENSLDAGATVIDI
                     EVEKGGAELCRVRDDGAGIAPTELLLALARHATSKIGSIDDLEAVRTLGFRGEALPSI
                     ASVSRMRMVSRPREAEVAHAVTAVDGALTGPAPHPHPPGTTVEVRDLFFNVPARRKFL
                     RAERTELAQVERLVERLALSRFDVGFRLSAARKVLADHAPARDPHACDGRVAAILGEE
                     FVANAMKLEHEGSGLRLRGWFCLPTYARSQADQQYFFLNGRPLRDKLVASAVRLAYRD
                     VLFHGRHPAYVLYLDMDPARVDVNAHPQKLEVRFREPGFVHDFLFRTLERALAETRPA
                     QPAMDAIPAARATWSSHVSASGSLFGAQARGALASTYDSAAHVPSWSRPAVADFAHAG
                     SLAATDLYRAFDEVREGSSADASATTPDAAEHPLGYAIAQLHGVYILAQARGGLVLVD
                     MHAAHERTTYERLKASTLGDRLPAQPLLVPVAVGVSVADADAAETQAELFASLGLDVD
                     RAGPAQLLVRSTPALLPNVDPATLLKDMIADLREQGRGAVTGAFDRALGTMACHHAVR
                     ANRRLTIAEMNALLREMECTVRSDQCVHGRPTWSFVSMDDLDRLFLRGR"
     gene            6504..7466
                     /gene="miaA"
                     /locus_tag="VD737_10070"
     CDS             6504..7466
                     /gene="miaA"
                     /locus_tag="VD737_10070"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HXW10954.1"
                     /translation="MTAGSQAPDAVCIMGPTCTGKTALALRLAARFPVEVVSVDSALV
                     YRGMDIGTSKPSAAERAAVRHHLIDVCDPAEPYSAGRFRRDALECIEEIRARGRVPLL
                     VGGTMLYFRALTHGLAPLPAADPAVRAAIDADAREAGWPRLHATLAEVDPAAAARIRP
                     NDAQRIQRALEVWRLTGRPLSELQAQAAPAPHALARFALQPVTREALYAGIDARFDAM
                     MAAGLLAEVRRLRDRGDLRPDLPSLRAVGYRQLWQHLEGGVALDHAVAAARQATRNLA
                     KRQLTWLRADPDISWIRALEDLDPVPISDAVTSACGKLGVRALC"
     gene            7570..7830
                     /gene="hfq"
                     /locus_tag="VD737_10075"
     CDS             7570..7830
                     /gene="hfq"
                     /locus_tag="VD737_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HXW10955.1"
                     /translation="MAKGQSLQDPFLNALRKERIPVSIYLVNGIKLQGQIDSFDQFVV
                     LLRNSVSQMVYKHAISTVVPARNVRLPIGDEDAPPPEDASAG"
     gene            7857..9203
                     /gene="hflX"
                     /locus_tag="VD737_10080"
     CDS             7857..9203
                     /gene="hflX"
                     /locus_tag="VD737_10080"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF008280.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HXW10956.1"
                     /translation="MFDRPGSGERAILVCVGLGRAPDADRIEEFQALARSAGAVVLDT
                     VVATRKSADPRFLVGSGKVAELRERIVALGADLVVIDGTLSPSQERNLEKELQCRVLD
                     RAGLILDIFAQRARTFEGKLQVELAQLQHMSTRLVRGWTHLERQRGGGIGLRGPGETQ
                     LETDRRLIGKRIKALNDRLDKVLVQRETTRQERRRAAVPTIALVGYTNAGKSTLFNRL
                     TRAQAYAADQLFATLDPTVRRLHLAPGLETALADTVGFVRDLPHELVAAFRSTLQEAR
                     EADLLLHVIDAADPERADRITQVNEVLESVGAGGLPQIAVYNKIDRTGEPPRVEMAEQ
                     GQIDRVWLSAHSGAGLDLLLQALHQHLGVDLCRCTLRLPPGAGRARARLFEAGAVVEQ
                     RMLDDGVLELDVSLRRPDLERICRDDGIDVPAECAPCAGEGRFLQSTGSQPTRGAA"
     gene            9270..>9943
                     /gene="hflK"
                     /locus_tag="VD737_10085"
     CDS             9270..>9943
                     /gene="hflK"
                     /locus_tag="VD737_10085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HXW10957.1"
                     /translation="MAWNDPGRNRNPWGNRPDKGTADLDEALRNLQRKLSQMFGGDGG
                     GDSDGRDDGRGGGGSGGGAIRGFGFGTVALVLVGIWALTGFYVVDAAERGVVTRFGEY
                     VETTEPGLRWHVPWPIEARQVVNVESIEGVQEQTRMLTSDENLVDINLAVQYRRAEPV
                     AYVFNVRDPDQTLKDVSESAIREIVGRSRLDFVLEEGRQEITARTKELVQRTLDSYKT
                     GIEVTTV"
BASE COUNT         1448 a         3496 c         3525 g         1474 t
ORIGIN      
        1 cgagttcgct cgggtcgtgt tcgcgtgagc gcagcgcttc gatgacgtcc gcgctgggtg
       61 gcgcgttgcc aagtgcgacc gcgacgttgc gcagccaacg ctcgtaaccc acgcggcgga
      121 tcgcgctgcc ttccgtgcag cgcaggaagt cttcttcgct ccagcggaac agctcggtca
      181 gccgcggtga gtcgagcccg tgacgggcgg cgaaatccgg ctcggcgctc agcttcgcgt
      241 agcggttcca ggggcacacg agctggcagt cgtcgcagcc gtagatgcgg ttgccgatcg
      301 ccacgcggaa ctcgggcgga atcgacccgt gatgctcgat cgtcaggtac gagatgcagc
      361 gtcgcgcgtc gagctcgtag ggcgccacga tcgcctgcgt ggggcacacg tcgatgcagg
      421 ccgtgcagct gccgcaatgc gaggtcacgg gcgcgtcggg tggcagtggg aggtcggtga
      481 acacttcgcc gaggaagaac cacgatccgt cgtggcggtc gagcaggttg gtgtgcttgc
      541 cgatccagcc gaggcctgcg ttgcgcgcga gcgctttctc gagcaccggc gcgctgtcgg
      601 tgaacgcccg gtagccggcc gcgttggcgg cctcggcgat ctgcccggcg agtcgtgcga
      661 ggcgcgcgcg caccagacgg tggtagtcgc ggccgagcgc atagcgcgag acgtacccga
      721 gtttgccgtc ggtgaggacg gtgacgggat cggcgcctgg cgggcggtag tccatgcgtg
      781 ccgagaccac gcgcaccgtg cccgggacga gttcggccgg acgcgtgcgc cgcgtcccgt
      841 ggcggcccat gtagtgcatg tcgccctggc gtcccagcgc gagccaggcc tcgagccgcc
      901 gttcgtccac cggcagttcg acgcccgcaa tgccgacctt ctggaagccg agttcacggc
      961 cccagtcctt gatcgaacgg gcgagtgcgt cgtagtcggt cacgtccatg gatgccgggc
     1021 gcgggcttgc ggcgacggga aggcccggca ggggcgccca gtataatgtg tcgcatgcaa
     1081 ccgatcccgg ccggcctcta cagcgcggca caagttcgcg agctcgaccg ctgcgccatc
     1141 gagcgccacg gcgtcccggg gtacgaactg atgacccgtg ccggccacgc agcgctcgac
     1201 gcgctgcgtc gcgcctggcc acaggctcgc tcggtctgcg tcctctgcgg tccgggcaac
     1261 aacggcggcg acggctacgt gctggcccgt ctcgcgcgcg cgcagaacct gcgcacgcac
     1321 gtgttcccgc tcaccgatcc cgcgcgactg cacggcgatg cgcgccgcgc gtacgaggat
     1381 ttcgccgcgg cgggcggcgc gtgcgaggag tggcatgcgt cgctgttgca gcaggtcgac
     1441 gtcgtcgtcg atgccgtctt cggcacgggc cttgcgcgcg cggtcgaagg ccgcgtggcg
     1501 cacgtattcg gctcggtgaa cgcggcggga cggcccgtgc tcgcgctcga cgttccgtcc
     1561 ggactggacg ccgacacggg tcacgcgctc ggcgtggcga ttcgtgcgga cgtgaccgtc
     1621 agcttcatcg gccgcaaggt cggctgctac gtcggcatcg gtcccgacat cgtcggtcgt
     1681 ctcgtgttcg acgacctcgg cgtaccgccg gccgtgtacg aagcggtttc cgcgaagcgg
     1741 cacctgctcg acgagtcgac ggtccacgcc gcgctgccgc ggcgttcgcg caccgcgcac
     1801 aagggccgcc acggccacgt gctgatcgtg ggcgccgccg ccggcatggg cggggccgta
     1861 cgactggccg gtgaagcggc attgcgtgtc ggtgcgggac tcgtcacggc ggccgtgcac
     1921 ccgcagagcc tcgtcgcgct gacgggtcgt ccagagctga tgaccgcggc gatcgccgac
     1981 tgcgccgaac tcgagcgtgc gctcgagcgt gcgagcatcg tcgcggtggg gcccgggctc
     2041 ggccagtcgc cgtggtcgat ggaagtgttc ggtgtcgtgc ttggttgcgg caagccgctg
     2101 gtggtcgatg ccgacgcgct gaacctgctc gcgctcgatc cccaggcgcg cgagtcgtgg
     2161 gtgctcaccc cgcatcctgg cgaggcggcc cgcctgctgc gcgcgtccac cgagcaggtc
     2221 caggccgatc gcctcggcgc cgccgtggag ttgcaggcac gcttcggagg caccgtcgtt
     2281 ctgaagggcg ccggctcgat cgtacaaggc gccggcggca cgagctggat ctgcgatcgc
     2341 ggcaatccgg gcatggccgc ggccggcatg ggcgacgtgc tgaccggggt gatcgcgggc
     2401 atcgccggcc agtgcgacga cctggcgctc gcggcacgcg ccggcgtcct ggtccatgcc
     2461 atggccggtg atcgggccgc gctgccgggt gaacgcggca ccacggccgg cgacgtgatc
     2521 gccgagttgc gtgcctgcgt gaatccgcgg tgagtctgca gttcatcgcc gccggtgccg
     2581 atggcacccg agagctcggc gcgaggctgg cggtcgcgtg gcaggcctcg gaggccgcgg
     2641 cgcgcgtggc gacactggtc acgctgtcgg gcgacctcgg cgccggcaag acgacgttcg
     2701 ccgcaggact gctgcaggcg ctcggtcacg cgggcccggt ccgcagcccg acctacacgc
     2761 tcatcgagcc ctatcgtttc ggcgcgcgcg acgtgcacca ctgcgattta tatcggctgc
     2821 gccatccgca ggaactcgac gacctgggcc tgcgcgacct gcgagctgcg gggtcgctgc
     2881 tcctggtcga atggccggag aaggccgaag gacggctcgg cgccccggat ctcgcggtca
     2941 cgttgcgata tgcgggcgac gcggcccgcg agctctcggt cgacgcgagg tccgaggtcg
     3001 ggcgggacgt gctcgtccac ctgtagcgac gcctcacgaa ccctacgtat ctacatcatt
     3061 tcattgaagt tggttcgggc gtgccttaga gtccgcgcca tgcgtgcccg cgagcctcgc
     3121 ctgatcgtcg ttttggtggc ctggctcggc ctgtcgctgg cggcgatgtc cgcgtcggcg
     3181 gcgacgtcgc gcgtcaagga cgtacgactg tggtcgggtc cggagggcac gcgcctggtc
     3241 atcgagctga ccgcaccggt cgagtacgac gtctttccgc tcggcaaccc gaaccgggtc
     3301 gtcatcgatc tcgccaatac gtcgctggcg ggccaggaac tcccggcagg ccaggggccc
     3361 gtccagcgcg tgcgctcggg gccgcagccg ggcaagggac tgcgattcgt gctcgacgtc
     3421 gagtcgccgc agccaccgag gcacttcgcc gtccgcccgg acggcaccgc cggccaccgc
     3481 atcgtcgtcg agttgcccgg aaagtccggg ccggtcgtcg cggccaccgc tgtagagccc
     3541 ggcacgtcgg gcgccggtgc gccgaccggc aacgaagtgc tggcgatggt cgccgagacc
     3601 tcgcgcgcgt ccgccgcgcc gatcgtcgag acgccaaggg tcagcgacgc gcccgcgacg
     3661 ccggcggtca agtcgctcgc gtcaaccgcc aagggtcgcg acctggtgat cgccatcgac
     3721 gccggtcacg gtgggcagga tcccggcgcc ctcggacgcg gcggcacgcg cgagaaggag
     3781 gtcacgctcg ccatcgcgcg caggctcgtc gcggaaatca accgcgaaga aggcatgcgt
     3841 gccgtgctga tccgcgacgg cgactatttc gtcaccctgg gcggacgcac gcgcaaggcc
     3901 cgcaaactcg gcgccgacat gttcatctcg atccacgccg actccgtgct caagcgcgac
     3961 gtgagcggtt cgtcggtcta cgtgctgtcg ctgcgcggtg cgagcgacga ggctgcccga
     4021 tggctcgcgg agtccgagaa cgcggcggac ctgcggggcg gcgtgccgct cgtcgatgac
     4081 gacctgctcg cgtcggtgct gctcgacgtg acgcagaagg aagcgatcag cgacagcgtg
     4141 gaggccgccg acaacgtgct ggcggcgctc gccagcgtcg gggcggtgca tggtgcgcga
     4201 cgcgtgcggc acgcaggctt cgtcgtgctc aagtccccgg agatcccgtc gatgctcgtg
     4261 gagaccggat tcatctccaa cgcgggcgac gagcggcggc tgcgcgacgg ctcgcaccag
     4321 cagaagatcg ccgcagcgat ccacgccggc atccgcagtt actggtacga caatccgccg
     4381 cccggcacgc gactcgccgc cctcgcggcc gctcgccgcg gcggctccgc cggcgcgccc
     4441 cagaccgtcg cggtcaaata gggacattcc ggatttccgc ggaaatgggg acactccgga
     4501 tttaacgtcc cacggccgcg gcagggggct gcccaggtgc cggctcgaac ggcgccgcgc
     4561 gctccgcccg tgaatccggc tgcgaaaaac agcagtgtcc ccgaatcgtc gtaaattcgg
     4621 agtgtcccca ttttccgcta tcctcccggc gccatgccga tccgggtcct ccccgagcaa
     4681 ctcgtccacc agatcgccgc cggcgaggtc gtcgagcgcc cggcgtcggt gatcaaggaa
     4741 ctcgtcgaga acagcctcga cgccggcgcg accgtgatcg acatcgaagt cgagaagggt
     4801 ggcgccgaac tctgccgggt ccgtgacgac ggtgcgggga tcgcgccgac ggaattgctg
     4861 ctcgcgctcg cgcgccacgc caccagcaag atcggcagca tcgacgacct cgaggcggtc
     4921 cgcacgctcg gcttccgtgg cgaggcgttg ccgagcatcg cgtcggtctc gcgcatgcgc
     4981 atggtgtcac gaccgcgcga ggcggaagtc gcgcacgccg tcaccgcggt ggacggggcg
     5041 ctgaccgggc cagcgccgca tccgcacccg cccggcacca cggtcgaggt gcgtgacctc
     5101 ttcttcaacg ttcccgcgcg ccgcaagttc ctgcgcgccg agcgcacgga actcgcccag
     5161 gtcgagcgtc tcgtcgagcg cctcgcgctc agccgcttcg acgtcggttt ccgcctgtcc
     5221 gccgcccgca aggtgctggc agaccacgcg ccggcgcgcg acccgcatgc gtgcgacggc
     5281 cgcgtcgctg cgatcctggg cgaggagttc gtcgcgaacg cgatgaagct cgagcacgaa
     5341 ggttccggcc tgcgcctgcg cggctggttc tgcctgccga cctacgctcg cagccaggcc
     5401 gaccagcagt acttcttcct gaacggccgg ccgctgcgtg acaagctggt cgcgagcgcc
     5461 gtgcgcctcg cctatcgcga cgtcttgttc cacggccgtc atccggccta cgtgctgtac
     5521 ctcgacatgg atcccgcgcg cgtcgacgtg aacgcgcacc cgcagaaact cgaagtgcgg
     5581 ttccgggagc cggggttcgt gcacgacttc ctgttccgca cgctcgaacg cgcgctcgcc
     5641 gagacgcgcc ccgcccagcc cgcaatggat gcgattcccg ccgcgcgcgc cacgtggtcg
     5701 tcgcacgtct ccgcatcggg atcgctgttc ggagcgcagg cgcgcggtgc gttggcgtcc
     5761 acgtacgaca gcgcggcgca cgttccatcg tggtcccggc ctgcggtcgc ggacttcgcg
     5821 cacgccggtt cgctggccgc caccgatctc taccgcgcct tcgacgaagt gcgcgaaggc
     5881 tcgagcgccg acgcgtccgc gacgacaccg gacgctgcgg agcacccgct cggctacgcg
     5941 atcgcgcagt tgcacggcgt gtacatcctg gcgcaggcgc gcggcggact ggtcctggtc
     6001 gacatgcacg ccgcgcacga gcgcaccacc tacgagcgcc tgaaggcctc cacgctcggc
     6061 gatcggctgc ccgcgcagcc gctgctggtg ccggtcgcgg tcggtgtatc ggtggccgat
     6121 gccgacgcgg cggagacgca agccgagctg ttcgcgagcc tcggcctcga cgtcgatcgt
     6181 gccgggcccg cacaactgct cgtccgctcg actcccgcgc tgttgccgaa cgtggatccg
     6241 gccacgctgc tcaaggacat gatcgcggac ctgcgcgagc agggacgagg cgcggtcacg
     6301 ggtgccttcg accgggccct cggcacgatg gcctgccacc acgcggtccg ggccaaccgg
     6361 cgcctgacga tcgccgagat gaacgcgcta ctgcgcgaga tggaatgcac ggtccgcagc
     6421 gaccagtgcg tgcacggccg gccgacctgg tctttcgtga gcatggacga cctcgaccgc
     6481 ctgttcctgc gcgggcggtg accttgactg ccggatccca ggcgccggat gccgtgtgca
     6541 tcatgggccc gacctgcacg ggcaagaccg cgctcgcgct gcggctggcg gcgcggttcc
     6601 ccgtcgaggt cgtcagcgtc gattccgcgc tggtgtaccg cggcatggac atcggcacct
     6661 ccaagccgag cgctgcggag cgggccgcgg tgcggcacca cctgatcgac gtctgcgacc
     6721 cggccgagcc gtactcagcg gggcgcttcc ggcgcgatgc gctcgagtgc atcgaagaga
     6781 tccgcgcgcg tggacgcgtg ccgctgctgg tcggcggaac gatgctgtat tttcgcgcac
     6841 tgacgcacgg gctcgcgccg ctgcctgcgg ccgatccggc ggtgcgcgca gcgatcgacg
     6901 ccgacgcgcg cgaggcgggc tggccacggc tccacgcgac gcttgcggag gtcgaccccg
     6961 ccgcggcggc ccgcatccgg ccgaacgacg cgcagcgcat ccagcgcgcg ctcgaagtct
     7021 ggcgactcac cgggcggccg ctttcggaac tgcaggcgca agcggcgccc gcgccacatg
     7081 cgcttgcacg atttgcgttg cagcccgtga cgcgcgaggc actctacgcg ggcatcgatg
     7141 cgcggttcga cgcgatgatg gccgcgggtt tgctcgccga agtgcgacgc ctgcgcgatc
     7201 gtggcgacct gcgaccggac ctgccgagcc tgcgtgccgt cggctaccgg cagctctggc
     7261 agcacctcga aggcggcgtc gcgctcgacc acgcggtcgc cgcggcgcgg caggcgaccc
     7321 gcaacctcgc gaagcgacaa ttgacgtggc tgcgggccga cccggacata agctggattc
     7381 gcgcacttga agatctcgat cccgtcccga tatccgacgc ggtgacgagt gcttgcggaa
     7441 aattgggcgt tcgcgccctg tgctaaactg gcgtcgttct tgcagggtcc cgcaacagat
     7501 cccgggccgt ttcaaggaag attccgcgca ggaacgaacg gcgaccgaca aaaaaaggag
     7561 actccaaaaa tggccaaggg ccagtccctt caggatccgt tcctgaacgc gctgcgtaag
     7621 gaacgcattc ccgtttccat ctacctggtc aacggcatca agctgcaggg ccagatcgac
     7681 tcgttcgacc agttcgtggt cctcctgcgc aacagcgtca gccagatggt gtacaagcac
     7741 gcgatctcga ccgtcgtgcc ggcgcgcaac gtgcgcctgc cgatcggcga cgaagacgcg
     7801 ccaccgcctg aagatgcgtc ggcaggctga cggcgcacag cgaggacacg ggttcattgt
     7861 tcgacaggcc gggtagcggc gaacgcgcga tcctggtgtg cgtgggcctg ggccgggctc
     7921 ccgacgccga ccgcatcgaa gaattccagg cgctggcgcg atcggccggt gccgtcgtgc
     7981 tcgacaccgt cgtcgcgacg cgcaagtccg ccgacccgcg tttcctggtc gggtccggca
     8041 aggtcgccga actgcgcgag cgcatcgtgg cgctcggcgc cgacctcgtc gtcatcgacg
     8101 gcacgctgag cccgagccag gagcgcaacc tcgagaagga gctccagtgc cgcgtgctcg
     8161 atcgcgccgg cctgatcctg gacatcttcg cgcagcgcgc gcgcaccttc gagggcaagc
     8221 tgcaggtcga gctcgcgcag ctgcagcaca tgtccacgcg actcgtgcgc gggtggacgc
     8281 acctcgagcg gcagcggggc ggcggcatcg gcctgcgggg tcccggtgaa acccagctcg
     8341 agaccgaccg ccgcctgatc ggcaagcgca tcaaggcgct gaacgaccgt ctcgacaagg
     8401 tgctcgtgca gcgtgagacg acgcggcagg aacgcaggcg cgcggccgtg ccgacgatcg
     8461 cgctcgtggg ctacaccaac gcgggcaagt cgacgctctt caaccggctg acgcgcgcgc
     8521 aggcctacgc ggccgaccag ctcttcgcga cgctcgatcc aacggtgcgc cggctgcacc
     8581 tggcgccagg cctcgagaca gcactcgccg acacggtcgg attcgtccgc gacctgccgc
     8641 acgagctggt cgcggcgttc cgctcgaccc tgcaggaggc ccgcgaggcg gacctgctgc
     8701 tgcacgtgat cgatgccgcc gaccccgagc gtgccgatcg catcacgcag gtcaacgaag
     8761 tgctcgaatc ggtcggcgcg ggcggactac cgcagatcgc cgtgtacaac aagatcgacc
     8821 gcaccggcga gccgccgcgc gtcgagatgg ccgagcaggg ccagatcgac cgggtctggc
     8881 tgtccgcgca cagcggcgcc ggtctcgacc tgctgctgca ggcgctgcac cagcacctcg
     8941 gcgtcgacct ttgccggtgt acgctccgac tgccgcctgg tgccggccgg gcacgggccc
     9001 gcctgttcga ggccggggcc gtggtcgagc agcgcatgct cgacgacggc gtgctcgagc
     9061 tcgacgtgag cctgcggcgc cccgacctcg aacgcatctg tcgcgacgac ggcatcgacg
     9121 tgccggcgga atgcgcgcct tgtgccggcg agggccggtt cctacaatcg accggatcgc
     9181 agcccacgcg cggcgcggca tgagtgccgc ccccgccggg ccgtgatccc gccgccgtcc
     9241 gactcgaagc acccggagca cccgtacgca tggcctggaa cgacccgggc aggaatcgca
     9301 atccctgggg taaccgtccc gacaagggca cggccgacct cgacgaggcc ttgcgcaacc
     9361 tgcagcgaaa actctcccag atgttcggcg gcgacggcgg cggcgacagc gacggccgtg
     9421 acgacggtcg cggcggcggc ggctcgggcg gcggggccat ccgcggcttc gggttcggta
     9481 ccgtggcgct cgtgctggtt ggcatctggg cgctcaccgg cttctacgtg gtcgacgcgg
     9541 ccgagcgcgg cgtcgtgacg cgcttcggcg aatacgtcga gaccaccgag cccggcctgc
     9601 gctggcacgt gccgtggccg atcgaggcgc ggcaggtcgt caacgtcgag tccatcgagg
     9661 gcgtgcagga gcagacgcgc atgctcacgt ccgacgagaa cctcgtcgac atcaacctgg
     9721 ccgtgcagta tcggcgcgcc gagcccgtcg cgtacgtctt caacgtgcgc gacccggacc
     9781 agacgctcaa ggacgtctcg gagagtgcca tccgcgagat cgtcggtcgc agccgcctcg
     9841 atttcgtgct cgaggagggt cgtcaggaga tcaccgccag gaccaaggaa ctggtgcagc
     9901 gcacgctcga ttcgtacaag accggcatcg aggtgacgac cgt
//
LOCUS       DASPFR010000381         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_357487, whole genome shotgun sequence.
ACCESSION   DASPFR010000381 DASPFR010000000
VERSION     DASPFR010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357487"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2073)
                     /gene="putA"
                     /locus_tag="VD737_10090"
     CDS             complement(<1..>2073)
                     /gene="putA"
                     /locus_tag="VD737_10090"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HXW10958.1"
                     /translation="ESLVRRLADAARFDPAESEAVLGTARRLVEGVRKAPKVKSGLDA
                     FLRQYDLSSQEGVILMCLAEALLRIPDDDTADRLIADKLRSGDWASHLGDSQSLFVNA
                     STWGLMLTGRIVRLDPDDVSAPQSAIGRVAGRIGEPIVRTAMRQAMKIMGHQFVMGRT
                     IKEALDNSLTGDNRRYRYTFDMLGEAALTSADANRYFEAYRAAIGALAARAGEYPDFE
                     SRPSISVKLSAMHPRFERAHRQRVLRDLTPRLVELCRLAREAGIALTLDTEESERLEL
                     TVELLAATAREPALAGWDGFGIAVQTYQRRALPLLRYLIRLANDTKRLLHVRLVKGAY
                     WDAEIKRGQERGLATYPVFTRKPNTDVSYLACARLVLEEGRNLYTQFATHNAHTVAAV
                     SHLARKHRREFEFQRLHGMGEDLYSQVTGDDGFALPCRVYAPVGNHEDLLPYLVRRLL
                     ENGSNSSFVNRIVNENEPIDAIIADPVRTVDGFETCVHPRIPQPLDIYMPERRNSAGV
                     HLVNGSVLASMAERMEHVSRRPWVATPLLAGEEAGGGASEAIRNPADHADVAGQVVAA
                     TPALVRRAIDVAVEAQPAWDATPAEVRARALDKAADLFEENTPELIAMCVREAGKTVP
                     DSVAEVREAVDFLRYYAARARVEFGAARRLPGPTGESNELSLHGRGVFTCISPWNFPL
                     ASFTGQVVA"
BASE COUNT          295 a          719 c          738 g          321 t
ORIGIN      
        1 gcgaccacct gcccggtgaa gctcgcgagc ggaaagttcc agggactgat gcaggtgaac
       61 acgccgcggc cgtgcaggct cagctcgttg ctttcaccgg tcggaccggg caggcgtcgc
      121 gcggcgccga attcgacgcg cgcacgcgcc gcgtaatagc gcagaaagtc cacggcctcg
      181 cggacctcgg cgacgctgtc cggcacggtc ttgcccgcct cgcgcacgca catcgcgatc
      241 agttccggcg tgttttcctc gaacaggtcg gcggccttgt cgagcgcgcg cgcgcggacc
      301 tccgcaggcg tggcgtccca tgccggctgc gcctccactg cgacgtcgat cgcgcgccgg
      361 acgagcgccg gcgtggccgc gacgacctgg cccgcgacgt cggcatggtc ggcgggattg
      421 cggatcgcct cgctggcccc gccgccggct tcttccccgg ccaggagcgg cgtcgctacc
      481 cacggtcgac gcgacacgtg ctccatgcgc tcggccatcg acgcgagcac ggagccgttc
      541 acgaggtgca cgccggcgga gttgcgccgt tccggcatgt agatgtcgag cggctgcggg
      601 atgcgcgggt gcacgcaggt ctcgaagccg tccacggtcc gcactgggtc tgcgatgatc
      661 gcgtcgatcg gctcgttctc gttgacgatg cggttcacga acgacgagtt cgacccgttc
      721 tcgagcagcc gccgcaccag gtacggcagc aggtcttcgt ggttgcccac cggcgcgtac
      781 acgcggcacg gcagcgcgaa gccgtcgtcg ccggtcacct gcgagtaaag gtcttcgccc
      841 atgccgtgca gccgctggaa ctcgaactcc cggcggtgct tgcgggcgag gtgcgaaacc
      901 gcggcgacgg tgtgcgcgtt gtgcgtggcg aactgcgtgt agagattgcg cccctcctcc
      961 agcaccagcc gcgcgcaggc caggtacgag acgtcggtgt tgggcttgcg cgtgaagacg
     1021 gggtaggtcg cgagcccgcg ctcctgcccg cgcttgatct cggcgtccca gtacgcgccc
     1081 ttgacgaggc gcacgtgcag cagccgcttc gtgtcgttgg caagccggat caggtaacgc
     1141 agcagcggca gcgcgcgccg ctggtaggtc tgcacggcga tgccgaagcc gtcccagcct
     1201 gcgagcgcag gttcgcgggc ggtggccgcc agcagttcga ccgtgagttc gagccgctcc
     1261 gattcctcgg tgtcgagcgt gagcgcaatg cccgcctcgc gcgccagccg gcacagctcc
     1321 acgagccgcg gcgtcaggtc gcgcagcacg cgctgccggt gcgcgcgctc gaagcgcggg
     1381 tgcatcgccg agagcttgac cgagatgctc ggccgcgatt cgaagtcggg gtactcgccg
     1441 gcacgtgccg cgagcgcgcc gatcgcggca cgataggcct cgaagtagcg attggcgtcg
     1501 gcggaggtga gcgcggcctc gccgagcatg tcgaacgtgt agcggtaccg ccgattgtcg
     1561 cccgtcagcg agttgtcgag cgcttccttg atcgtgcggc ccatgacgaa ctggtgaccc
     1621 atgatcttca tggcctggcg catggcggtg cgcacgatcg gctcgccgat gcggccggcc
     1681 acgcgaccga tcgcgctttg cggcgccgag acgtcgtcgg ggtcgagccg cacgatgcgc
     1741 cccgtcagca tcaggcccca ggtggaggcg ttgacgaaca gcgactggct gtcgcccagg
     1801 tggctcgccc agtcgcccga gcgcagcttg tccgcgatga gacggtcggc ggtgtcgtcg
     1861 tcggggatgc gcagcagcgc ctcggcgagg cacatcagga tcacgccttc ctgcgacgac
     1921 aggtcgtact gtcgcaggaa cgcatcaagc cccgacttga ccttcggggc cttgcgcacg
     1981 ccttcgacca gccggcgcgc ggtcccaagc acggcttcgc tctccgccgg atcgaaccgg
     2041 gccgcatcgg cgaggcggcg gaccagcgac tcc
//
LOCUS       DASPFR010000382         9309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_235065, whole genome shotgun sequence.
ACCESSION   DASPFR010000382 DASPFR010000000
VERSION     DASPFR010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9309
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235065"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /gene="pyrB"
                     /locus_tag="VD737_10095"
     CDS             complement(<1..317)
                     /gene="pyrB"
                     /locus_tag="VD737_10095"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF014749.4"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HXW10959.1"
                     /translation="MKTDTGRDLQRDHAGRLRHLLTLEGLSADEIRGLLDFAQFYVRH
                     PGDIAARDGSLAGRTVANLFFEPSTRTRVSFELAAKRLGADVVNLDMQSSSRVKGETV
                     LDT"
     gene            complement(443..868)
                     /gene="ruvX"
                     /locus_tag="VD737_10100"
     CDS             complement(443..868)
                     /gene="ruvX"
                     /locus_tag="VD737_10100"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HXW10960.1"
                     /translation="MVLAFDYGARRIGVAVGQTLTGSATAVGTVAARDGTPDWPAVDT
                     CIAQWGPAQLLVGLPYNMDGSDTSTTAACRRFGEKLARRTGLPVEFVDERLTSAAAYD
                     ELRGERRSGARSRRIRPQDIDANAARLILETWMRGRGSG"
     gene            complement(928..1506)
                     /locus_tag="VD737_10105"
     CDS             complement(928..1506)
                     /locus_tag="VD737_10105"
                     /inference="COORDINATES: protein motif:HMM:NF001266.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HXW10961.1"
                     /translation="MRRYCGRVATYLTNQFLIAMPAMEDANFAQTVTLVCEHSERGAL
                     GIVINRTLPMTLGEVFDQLGLDSSKSRVNEQPVLRGGPVQTERGFVLHSPSGKWESSL
                     PFSEKMHLTTSRDILDALAAGQGPESAVIALGYAGWDAGQLEEEMARNAWLTVDADER
                     VLFETPVEQRWHAAARLLGVDLRSLSSDAGHA"
     gene            complement(1503..2396)
                     /locus_tag="VD737_10110"
     CDS             complement(1503..2396)
                     /locus_tag="VD737_10110"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXW10962.1"
                     /translation="MSTSLLSRRRQRDPAQDRLATAIFVAALVHGLVIMGVRFGAPIV
                     ADPPLPTLEILLVPSGPDDLQPNDDAAYLAQRTQKGSGTGEAAKRSSLPEARPFDPAE
                     GDSELERAAMEIARGQGSALARRRLDAARLPTGATEAIEQAPVYHAQPAPPTVGLDAA
                     ANDQELHLRGQRSADGKLLADTRESAIAAYLDGWKRRIERVGTLNFPNEARRRHMSGN
                     PVLEVAIRADGSLESVVVRRTSGHRELDGAAVDIVRLAAPFDPFSTALRGRYPVLRFA
                     YEWQFLNGRLGGEGAVFPTEP"
     gene            complement(2402..3361)
                     /gene="gshB"
                     /locus_tag="VD737_10115"
     CDS             complement(2402..3361)
                     /gene="gshB"
                     /locus_tag="VD737_10115"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003195061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HXW10963.1"
                     /translation="MPNTTLRIAVVMDPIEHIKPRKDTTLAMLLAAQQRGWELHYLEL
                     DDIWLRDGVAMGRAHPISVREHESEWFTRGEPVVTRLGEFAAILMRKDPPFDTEYIYS
                     TYILERAEMQGALVVNRPQGLRDMNEKVYTAWFPQCCAPTLITRDMTDMHAFLREHGR
                     AVCKPLYGMGGRSIFVVDLGDKNANVVFETLTEYGTRYAIVQRYIPDIVTTGDSRVIL
                     VDGEPAPYALARIPTEADNRGNLAAGAKGVGRPLDERDRWLAGQIGPVLRERGMLFVG
                     LDVIGGYVTEINVTSPTGVRELGQQFGIDIAGGLMDAIARRLG"
     gene            3533..3943
                     /locus_tag="VD737_10120"
     CDS             3533..3943
                     /locus_tag="VD737_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014720321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXW10964.1"
                     /translation="MENTAASNSRPRLAGLKVLVIDDSKTIRRTAETLLAREGCEVFT
                     AVDGFDALSKVADHQPDIVFVDIMMPRLDGYQTCSLIKHNKVFRSIPVIMLSSKDGLF
                     DRARGRIVGSEHYLTKPFTREELLSAIETHVHRA"
     gene            3961..4326
                     /locus_tag="VD737_10125"
     CDS             3961..4326
                     /locus_tag="VD737_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXW10965.1"
                     /translation="MALVLIVDDSPTDVHVMQKALEQHGFRTAAAADGSEGLRLAREM
                     HPDLILMDIVMPGVNGFQATRELANDPQTRKIPVIMVTSKSQESDRVWGLRQGAVDYI
                     VKPVATEQLVRKAQAAIAT"
     gene            4331..4897
                     /locus_tag="VD737_10130"
     CDS             4331..4897
                     /locus_tag="VD737_10130"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HXW10966.1"
                     /translation="MHGATGLRSLRERPFELLREMDRRARVAAQGQPESVAAGPEWVG
                     IAFRLGGEAFLLAREETREVLAHPTAVTRVPGARPWIRGLSNVRGQLLPVVDLRAFLG
                     AGASAVTRTSRVLVANHREIPAGLVVDEVMGFRRFFESEFSADLPPTLLRCERYLAGA
                     FKRGADVWPVFSVKTLLESQQFMQAAAS"
     gene            4929..7040
                     /locus_tag="VD737_10135"
     CDS             4929..7040
                     /locus_tag="VD737_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXW10967.1"
                     /translation="MTNRIDAARLQPTLLGVALAAALLAALALLLTGRAMQQQSQLRE
                     HMASLTSLSQNIPLQAGAAVRGSAPAFDALAASRARLERVIDEINAGKSALGLLASPS
                     ARVLGGENGWAALLEQSQAVLDGRQAALQAQKAAADVRETTPRLLSTAASVLKTMGPG
                     RPEAVNRSFERFELRAQAVEQDLAALADGTAPVEAAARRLTDSLDFMQQVVTGIAGEP
                     NTLGLAAATGEAATAAQALYTAFRSEAEQVRAVIGLADQLEQMQAARNALTETGATLG
                     AGLAKIDTSGAIRSGGPLASPWLPLFFIVVTVLALGGAAWLQRELAVLRKAAEHQARQ
                     NERNQQAILRLLDELSSLADGDLTVQATVTEDITGAIADSINYAIDALRELVVTINDS
                     AVKLDAATRQTQALSTHLAKASSAQSKQIGAASESIAAMAASTEEVSGNAERSADVAR
                     HSVDIAHKGGDAVRRTIDGMNAIRENIQETSKRIKRLGESSQEIGNIVELINDIAEQT
                     NILALNASIQASMAGEAGRGFAVVADEVQRLAERAATATKQIEVLVRTIQADTNEAVV
                     SMERTTTDVVGGALLAENAGAALEEIEQVSNQIASLVQNISASSRQQAGVSQSISRNM
                     QVLREISSQTAESTTATSTSIGKLAELAAQLRKSVAGFRLPGAMGSGTGEYQVLRAED
                     APATPAATSGSNVRRIGSLAG"
     gene            7055..>9309
                     /locus_tag="VD737_10140"
     CDS             7055..>9309
                     /locus_tag="VD737_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HXW10968.1"
                     /translation="MTNESLPLASVDEAHASLQVISREVATELNEARVALEAFAEQPE
                     DRGALHRFAAHIHLARGALRLAEVYGGSLLAEEMEFVARYVDAHSGEGKADADGLDAL
                     MRSMEQLPAYVERVATGARDLPILLLPLLNDLRAVRGAALLSEGTLLLLNLKSDAQAR
                     PDSPHTGDRQVAELARKLRPRFQLALLGWIRGERVDEQLHHLSEIAAQFEAAASTQPL
                     YQLWWVVQAVIEALRTGGIEATVSVKRLLGHVDREMKRLADLGEARYAETPPADVLNN
                     LLYYVAQSSTVGPVVADVRQSFSLQDMVATQDAQATETADTLSAPSVRLMRTVAAAIR
                     EDLTRVKDVLDIFVRKGATQVDDLAPQLEMLRKISDTLGVLGLGALRARVEGELESLK
                     AIVERRIPPDDTVLLGIAAALIAVEDSLDSQLVRLILPDRDAAQADAGEPTDEEFRAV
                     QEAVLRECIVNMARIKEAVTHSLSAPAEAQGLDQVPQLVRGITAGLLMLGKQRAVEVM
                     EGVGRALGGIVRPDPATLAPARLERLADAVVAVEYYMETLQAGRADQWQMLEAAEARL
                     AELEPTGARVLPWREPAAPAEAAVDRTQRIVPLPAAEPSTGHAGRPATAPPPPVTAAT
                     TPTTVDPEFLQLFVEEARENVERLARLFPQWEQNPLDAEALRDLRRAFHTLKGSGRMV
                     GARRVGEFSWSIESLLNRVISQTLPRSPDIVAVVRDAVAVLPRLVGELEDAAAPAADV
                     AGLMTRADDLSG"
BASE COUNT         1405 a         3312 c         3263 g         1329 t
ORIGIN      
        1 atcgtgtcga gcaccgtctc gcccttgacg cgcgacgagg actgcatgtc gaggttcacg
       61 acgtcggcgc cgagccgctt ggccgcgagt tcgaacgaca cgcgcgtgcg cgtgctcggc
      121 tcgaagaaca ggttggcgac cgtgcgaccg gcgaggctgc cgtcgcgcgc ggcgatgtcg
      181 cccgggtggc gcacgtagaa ctgcgcgaag tcgagcagcc ccctgatctc gtccgcggac
      241 agtccctcga gcgtgagcag gtgacgcagg cgcccggcgt ggtcgcgctg caggtctcgg
      301 ccggtgtcgg tcttcaagca ggcctccccg ttggggaaag aggtcagtgg tcggtggcta
      361 gtggctagtg gctagtgcga gagtgcttcg cgcgtattcg cggccctcgg cgccgatccg
      421 actgaccact agccactgac cactagccac ttccccttcc tctcatccac gtctccagga
      481 tcagccgagc ggcgttcgcg tcgatgtcct gcggccggat cctgcgcgag cgcgcgccgg
      541 agcgacgctc gccgcgcagc tcgtcgtacg cggcggccga cgtcaggcgc tcgtcgacga
      601 attccacggg caggccggtg cgccgcgcga gcttctcgcc gaagcggcgg caggcagccg
      661 tcgtgctggt gtccgaaccg tccatattat aggggagccc cacgagcagc tgcgccggcc
      721 cccattgggc gatgcacgta tcgacggcgg gccagtccgg cgttccgtcg cgggcggcca
      781 ccgtgcccac ggccgtggcg gagccggtca gcgtctgccc gacggccacg ccgatccgtc
      841 gcgccccgta atcgaacgcg agcaccaccc ggcccgcaac ccgggcgagg cccgggttct
      901 ggtcgctttc cggggggctg gacgggttca ggcgtgccct gcgtcgctgc tgagcgagcg
      961 caagtcgaca ccgagcagcc gggccgccgc atgccagcgc tgctcgaccg gtgtctcgaa
     1021 cagcacgcgt tcgtcggcgt ccacggtgag ccaggcgttg cgcgccattt cctcctcgag
     1081 ctggccggcg tcccagcccg cgtaaccgag cgcgatcacg gcgctctcgg ggccttgccc
     1141 cgcggcgagt gcgtcgagga tgtcgcggga cgtcgtcagg tgcatcttct cggagaacgg
     1201 caacgacgac tcccacttgc cggacggcga gtgcagcacg aagccgcgct cggtctggac
     1261 gggtccgccg cgcagcacgg gctgctcgtt gacgcgcgac ttgctcgagt cgaggccgag
     1321 ctggtcgaac acctcgccga gcgtcatcgg cagcgtccgg ttgatgacga tgccaagcgc
     1381 gccacgctcg ctgtgctcgc agaccagcgt gacggtctgc gcgaagttcg cgtcttccat
     1441 cgccggcatg gcgatcagga actggttggt gaggtacgtc gccacgcggc cacagtagcg
     1501 tctcaaggct cagtcgggaa gaccgcgcct tcgccgccca gtcgcccgtt caggaactgc
     1561 cactcgtagg caaaccggag caccggatac cgcccgcgca gcgcggtcga gaacgggtcg
     1621 aacggcgcgg ccagccgcac gatgtcgacc gcggctccgt ccagttcccg gtgcccgctg
     1681 gtacgccgga ccacgacgct ctcgagcgag ccgtcggccc ggatcgcgac ctcgagcacg
     1741 gggttgccgg acatgtgccg gcgccgtgcc tcgttcggaa aattgagcgt gccgacgcgt
     1801 tcgatccgtc gtttccagcc gtcgaggtaa gcggcgatcg ccgactcacg cgtgtctgcc
     1861 agcaatttgc cgtccgccga tcgctgcccg cgcaggtgca gttcctggtc gttggcggcc
     1921 gcgtcgagcc ctacagtggg cggtgccggc tgcgcgtggt acacgggcgc ctgctcgatc
     1981 gcctcggtgg cgccggtcgg cagccgcgcg gcgtccaggc ggcgtcgcgc gagcgccgag
     2041 ccctggccgc gcgcgatctc catcgccgcc cgctcgagtt cggaatcccc ctcggccggg
     2101 tcgaacggcc gggcctccgg cagcgagctg cgcttcgcgg cctcgccggt gccgctgccc
     2161 ttctgagtgc gttgcgccag gtaggccgcg tcgtcgttcg gctgtagatc gtccggcccg
     2221 ctcggcacga gcaggatctc cagcgtcggc aggggcgggt ccgccacgat cggcgcaccg
     2281 aatctcacgc ccatgatcac gagcccgtgc accagggcgg cgacgaagat ggccgtcgcg
     2341 agccggtcct gcgccgggtc gcgctgtcta cgacgcgaga gaagtgacgt cgacatcgcc
     2401 ttcatccgag acgacgcgcg atcgcgtcca tcagcccgcc tgcgatgtcg atgccgaact
     2461 gctggccgag ctcgcgtacg cccgtcgggc tcgtcacgtt gatttccgtg acgtagccac
     2521 cgatcacgtc gagacccacg aacaacatgc cgcgctcgcg cagcacgggg ccgatctgtc
     2581 cggcgagcca gcggtcgcgc tcatcgagcg gacggccgac gcccttggcg cctgcggcca
     2641 ggttgccgcg gttgtccgct tcggtcggga tgcgtgccag ggcgtaaggg gcgggctcgc
     2701 cgtccaccag gatgaccctc gagtcgccgg tcgtgacgat gtcggggatg tagcgttgca
     2761 cgatcgcgta tcgggtgccg tattcggtga gcgtttcgaa gacgacgttc gcattcttgt
     2821 cgccgaggtc caccacgaag atcgagcggc cgcccatgcc gtacagcggc ttgcagacgg
     2881 cgcggccgtg ctcgcgcagg aacgcgtgca tgtccgtcat gtcgcgcgtg atcagcgtcg
     2941 gcgcgcagca ctgcgggaac caggccgtgt agaccttctc gttcatgtcg cgcaggccct
     3001 gcgggcggtt gacgaccagc gcgccctgca tctcggcccg ctcgaggatg tacgtcgagt
     3061 agatgtactc cgtgtcgaat ggcggatcct tgcgcatcag gatcgcggcg aactcgccga
     3121 gccgcgtcac caccggctcg ccgcgcgtga accactcgct ctcgtgctcg cggaccgaga
     3181 tcggatgcgc ccggcccatc gcgacaccgt cgcgcagcca gatatcgtcg agctcgaggt
     3241 aatgcagctc ccagccgcgc tgctgcgcgg ccagcagcat cgcgagcgtg gtgtccttgc
     3301 gcggcttgat gtgctcgatc ggatccatga cgacggcgat gcgcaaggtc gtgttcggca
     3361 tgcgggctcc ggtcgcgcgt ggccgcctga aaaggccact gtgatgcccg tcatggtatc
     3421 gaaagccgcc aatggcaggg gggtgaccaa tatgttaaaa cgccagcgcg agcagtcgtc
     3481 cggcgcatag cgtccactgc cgcgcggcat cgcatcgagg ggtccttcag gcatggaaaa
     3541 cacggcagcc agcaacagcc gcccgcggct ggccggcctc aaggtgctcg tcatcgacga
     3601 cagcaagacg atccgccgca cggcggagac gctgctggcg cgcgaaggat gcgaggtctt
     3661 caccgccgtc gacggtttcg atgcgctctc gaaggtcgcc gaccaccagc ccgacatcgt
     3721 gttcgtcgac atcatgatgc cgcgactcga cggctaccag acctgctcgc tgatcaagca
     3781 caacaaggtg ttccgctcga ttcccgtcat catgctgtcc agcaaggacg gcctgttcga
     3841 ccgggcccgc gggcgcatcg tcggctccga gcactacctg accaagccgt tcacgcgcga
     3901 agaactgctg agcgcgatcg agacccacgt acaccgcgcc tgatcccgga ggacaccccc
     3961 ttggcactgg ttctgatcgt ggacgactcg ccgaccgacg ttcacgtgat gcagaaggcg
     4021 ctcgagcagc acggcttccg gacggccgcc gcggccgacg gcagcgaggg cctgcgcctc
     4081 gcgcgcgaga tgcaccccga cctgatcctg atggacatcg tgatgcccgg cgtcaacggt
     4141 ttccaggcca cgcgcgagct cgcgaacgat ccgcagacgc gcaagatccc ggtgatcatg
     4201 gtcacgtcga agtcgcagga gtccgaccgc gtctggggcc tgcgccaggg ggcggtcgat
     4261 tacatcgtca agccggtcgc gacggagcaa ctggtccgca aggcgcaggc cgcgatcgcc
     4321 acctgagccc atgcacggcg caaccggcct gcgttcgctg cgcgaacgcc ctttcgaact
     4381 gctgcgggag atggaccgcc gcgcccgcgt cgccgcgcag ggccagcccg aatcggtcgc
     4441 ggccggtccc gaatgggtcg gcatcgcctt ccggctcggc ggcgaagcgt tcctgctggc
     4501 gcgcgaggag acgcgcgagg tgctcgcgca cccgaccgcc gtcacgcgcg tgcccggggc
     4561 ccggccgtgg atccgcgggc tctccaacgt gcgcggccag ctactgccgg tcgtcgacct
     4621 gcgcgcgttc ctcggtgccg gcgcgagcgc ggtgacgcgc accagccgcg tgctggtcgc
     4681 gaaccaccgc gagatccccg cgggcctcgt cgtcgacgag gtcatgggct tccggcgctt
     4741 cttcgaaagc gaattctccg ccgacctgcc gccgacgctg ctccggtgcg agcgttacct
     4801 cgcgggcgcc ttcaagcgcg gcgccgacgt ctggccggtg ttcagtgtca agacgctgct
     4861 cgagagccag cagttcatgc aggccgcggc ttcgtgacgc ggccgaagac gacgatcatg
     4921 gtgacgacat gaccaacaga atcgacgccg cccggctgca gcccacgctg ctcggggtgg
     4981 cgctcgccgc cgcgctgctc gcggcgctcg ccctgctgct gaccggccgc gcgatgcagc
     5041 agcagtcgca gctgcgcgaa cacatggcgt cgctcacctc gctttcgcag aacatcccgc
     5101 tgcaggccgg tgccgcggtg cgcggatccg cgccggcctt cgacgcgctc gccgcttcgc
     5161 gcgcgcgcct cgagcgggtg atcgacgaga tcaacgcggg caagagcgcg ctcggcctgc
     5221 tggcgagccc cagtgcccgc gtgctcggcg gcgagaacgg ctgggccgcg ctgctcgaac
     5281 agtcacaggc cgtgctcgac ggacgccagg ccgcgctgca ggcgcagaag gcggcggccg
     5341 acgtgcgcga gacgacaccg cggctcctct cgacggcggc gagtgtcctc aagacgatgg
     5401 gccccggtcg ccccgaggcg gtcaaccgca gcttcgagcg gttcgagctg cgtgcgcagg
     5461 cggtcgagca ggacctcgcg gcgctcgcgg acggcaccgc gcccgtggag gccgccgcgc
     5521 gcaggctgac cgacagcctc gacttcatgc agcaggtcgt gaccggcatc gcgggcgagc
     5581 cgaacacgct cgggctcgca gcggcgaccg gcgaggctgc cacggcggcg caggcgctct
     5641 acaccgcgtt ccgcagcgag gccgaacagg tgcgcgcggt catcggcctc gccgaccagc
     5701 tcgaacagat gcaggcggcg cgcaacgccc tgaccgagac cggcgcgacg ctgggcgcgg
     5761 gcctcgcgaa gatcgacacg agcggggcga tccgctccgg cggtccgctc gcgagcccgt
     5821 ggttgccgct cttcttcatc gtggtgaccg tgcttgcgct cggcggcgcc gcgtggctgc
     5881 agcgcgagct ggccgtgctg cgcaaggccg ccgagcacca ggcgcgccag aacgagcgca
     5941 accagcaggc gatcctgcgg ctgctcgacg agctctcgag cctcgccgac ggcgacctga
     6001 ccgtgcaggc cacggtcacc gaggacatca ccggcgcgat cgccgactcg atcaactacg
     6061 cgatcgacgc gctgcgcgag ctcgtcgtca cgatcaacga ctcggccgtc aagctcgacg
     6121 ccgccacccg gcagacgcag gcgctctcga cccacctggc gaaagcctcc tctgctcagt
     6181 ccaagcagat cggcgcggcc tcggaatcga ttgcggccat ggccgcctcg accgaagaag
     6241 tctccggcaa cgccgaacgc tcggccgacg tcgcccgcca ctcggtcgac atcgcgcaca
     6301 agggcggcga tgcggtccgc cgcacgatcg acggcatgaa cgcgatccgc gagaacatcc
     6361 aggagacgtc gaagcgcatc aagcgcctgg gtgagagctc gcaggagatc ggcaacatcg
     6421 tcgagctgat caacgacatc gccgagcaga ccaacatcct cgcgctcaac gcgtcgatcc
     6481 aggcgtcgat ggccggggag gccggccgcg ggttcgccgt ggtcgccgac gaggtgcagc
     6541 ggctcgccga gcgcgccgcc accgcgacca agcagatcga ggtgctggtg cgcacgatcc
     6601 aggccgacac caacgaagcc gtcgtctcga tggagcgcac cacgacggac gtcgtgggcg
     6661 gtgcgctgct cgcggagaac gccggggcgg cgctcgagga gatcgagcag gtttccaacc
     6721 agatcgcgag cctggtccag aacatctcgg cgagctcgcg ccagcaggcc ggcgtgtcgc
     6781 agagcatctc gcgcaacatg caggtgctgc gcgagatcag ctcacagaca gccgagagca
     6841 ccacggccac ctcgacctcg atcggcaagc tcgccgagct cgctgcgcag ttgcgcaagt
     6901 cggtcgccgg cttccgcctg cccggcgcga tgggcagcgg gaccggcgag taccaggtcc
     6961 tgcgcgccga ggatgcgccg gccacgccgg cggcaacgag cggcagcaac gtgcgccgca
     7021 tcggcagcct ggcgggctga cggcggagtc gatcgtgacg aacgaatccc tgccgctcgc
     7081 ttcggtcgac gaggcgcacg cctcgttgca ggtcatctcc cgcgaggtcg cgaccgagct
     7141 gaacgaggcc cgtgtcgcgc tcgaggcgtt cgccgagcag cccgaggacc gtggtgcgct
     7201 gcaccgcttc gcggcccaca tccacctggc acgcggcgcg ctgcggctgg ccgaggtgta
     7261 cggcggctcg ctgcttgcgg aggaaatgga attcgtcgcg cgctacgtcg acgcgcactc
     7321 gggcgaaggc aaggccgatg ccgacggcct cgacgccctg atgcgctcga tggagcagtt
     7381 gcccgcgtac gtggagcgcg tggcgaccgg cgcgcgcgac ctgccgatcc tgctgctgcc
     7441 gctgctgaac gacctgcgcg ccgtgcgcgg tgcggcgctg ctgtccgaag gcaccctgct
     7501 gctgctgaac ctgaagtccg acgcacaggc gcgcccggac tcgccgcaca ccggcgaccg
     7561 ccaggtcgcg gagctcgccc gcaagctgag gccacgcttc cagctcgcgc tgctcgggtg
     7621 gattcgcggc gagcgcgtcg acgagcagct gcaccacctg tccgagatcg ccgcgcagtt
     7681 cgaggccgcg gcgtcgacgc agccgctgta ccagctctgg tgggtcgtgc aggccgtgat
     7741 cgaggcgctg cgcacggggg gcatcgaggc caccgtctcc gtcaagcgcc tgctcggcca
     7801 cgtcgaccgc gagatgaagc ggctcgccga cctgggcgag gcacgctacg ccgagacgcc
     7861 gccggccgac gtgctcaaca atctgctgta ttacgtggcg cagagttcga cggtggggcc
     7921 cgtcgtggcg gacgtgcgcc agtcgttcag cctgcaggac atggtggcga cccaggacgc
     7981 gcaggccacc gaaaccgcgg acacgctctc tgcgccgagc gtgcggctca tgcgcaccgt
     8041 ggccgccgcg atccgcgagg acctgacgcg cgtcaaggac gtcctcgaca tcttcgtgcg
     8101 caagggcgcg acccaggtcg acgacctcgc gccgcagctc gagatgctgc gcaagatcag
     8161 cgacacgctc ggcgtgctgg gcctcggcgc gctacgggcg cgcgtggaag gcgaactcga
     8221 atctctcaag gccatcgtcg agcggcgcat cccgccggac gacacggtgc tgctcggcat
     8281 cgcggccgcg ctgatcgcgg tcgaggacag cctcgactcg cagctcgtgc ggctgatcct
     8341 cccggaccgg gacgccgcgc aggccgacgc gggcgagccg accgacgagg aattccgcgc
     8401 ggtacaggaa gcggtgctgc gcgagtgcat cgtcaacatg gcccgcatca aggaagccgt
     8461 cacgcactcg ctctcggcgc cggccgaggc gcaggggctc gaccaggtgc cgcagctggt
     8521 gcgtggcatc accgccgggc tgctgatgct cggcaagcag cgcgcggtcg aggtcatgga
     8581 gggcgtcggt cgcgcgctcg gcggcatcgt ccgccccgac ccggccacgc tcgcacctgc
     8641 gcggctggag cgtctcgccg acgcggtcgt cgcggtcgag tactacatgg agacgctaca
     8701 ggcgggccgg gccgaccagt ggcagatgct ggaggccgcc gaggcgcggc tggccgagct
     8761 cgagccgacg ggggcccgcg tgctgccgtg gcgggaaccc gccgcgcccg ccgaagcggc
     8821 cgtcgatcgc acgcagcgga tcgtgccgct gcctgccgcc gagccgtcca ccggccacgc
     8881 cggaaggccg gcgacggcgc cgccaccgcc tgtcacggcc gccacgacgc cgacgacggt
     8941 cgatcccgag ttcctgcaac tgttcgtcga ggaagcgcgc gagaacgtcg aacgcctggc
     9001 gcggctgttt ccgcagtggg aacagaaccc gctcgacgcc gaggcgctgc gcgacctgcg
     9061 tcgcgctttc cacacgctca agggcagcgg ccgcatggtc ggcgcgcgcc gcgtcggcga
     9121 gttctcctgg agcatcgaga gcctgctgaa tcgcgtgatc agccagacgc tgccgcgctc
     9181 cccggacatc gtggccgtgg ttcgcgacgc ggtggcggta ctgccacggc tggtcggcga
     9241 actcgaggac gcggccgcgc ctgccgcaga cgtcgcgggg ctgatgacgc gtgccgatga
     9301 cctgtccgg
//
LOCUS       DASPFR010000383         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_601338, whole genome shotgun sequence.
ACCESSION   DASPFR010000383 DASPFR010000000
VERSION     DASPFR010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601338"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..386)
                     /gene="rpsN"
                     /locus_tag="VD737_10145"
     CDS             complement(81..386)
                     /gene="rpsN"
                     /locus_tag="VD737_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HXW10969.1"
                     /translation="MAKTSMIEREKRRAKTVKKYAAKRARIKEQIRDPKASQEDRMAA
                     VEALQKLPRDSSPIRARSRCAITGRSRGNYSKFGLGRVKLREATMRGDVPGLRKASW"
     gene            complement(390..938)
                     /gene="rplE"
                     /locus_tag="VD737_10150"
     CDS             complement(390..938)
                     /gene="rplE"
                     /locus_tag="VD737_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HXW10970.1"
                     /translation="MAESARLQKHYREVVKPKLRSELGLVSDMQVPRITKITVNMGVG
                     EAVADKKVMDAATGDLAKITGQKPSVRKSKKSVATFKIRENLPIGCMVTLRGARMYEF
                     LDRLINVAMPRIRDFRGVNPRSFDGQGNYSFGVKEQIIFPEIAYDQIDQVRGMDITIT
                     TSAGDDKRGRALLEAFNFPFRK"
     gene            complement(954..1271)
                     /gene="rplX"
                     /locus_tag="VD737_10155"
     CDS             complement(954..1271)
                     /gene="rplX"
                     /locus_tag="VD737_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HXW10971.1"
                     /translation="MRKIRKGDSVVVTAGREKGRTGTVVGVQADGRVLVENVNMVKKH
                     QRPNPQRNVQGGIVQKEAPLHASNVALLNPATKKADRVGVRTLKDGRKVRFFKSNGEL
                     VDA"
     gene            complement(1268..1636)
                     /gene="rplN"
                     /locus_tag="VD737_10160"
     CDS             complement(1268..1636)
                     /gene="rplN"
                     /locus_tag="VD737_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HXW10972.1"
                     /translation="MIQLRTRLQAADNSGARELMCIKVMGGTRRRYAGVGDVIKVSIK
                     DAIPRGKVKKGEVYDAVVVRTRRGVRRADGSLIRFDGNAAVLLTNKLEPIGTRIFGPV
                     TRELRSVAFMKIISLAPEVL"
     gene            complement(1653..1922)
                     /gene="rpsQ"
                     /locus_tag="VD737_10165"
     CDS             complement(1653..1922)
                     /gene="rpsQ"
                     /locus_tag="VD737_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HXW10973.1"
                     /translation="MTESAKKEARTLTGTVVSNKMDKTISVAVERLIKHPTYGKYVRR
                     TTKLLAHDEKNECNEGDTVAISECRPLSRNKSWTLVRVLERAPAE"
     gene            complement(1922..2161)
                     /gene="rpmC"
                     /locus_tag="VD737_10170"
     CDS             complement(1922..2161)
                     /gene="rpmC"
                     /locus_tag="VD737_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HXW10974.1"
                     /translation="MSRKNVKERARTLTALRGKTRAELREELAELRREQFNLRMASAS
                     GQPARPDQHAKAMGKIARVKTVLNEMDRAQAGSKP"
     gene            complement(2161..2574)
                     /gene="rplP"
                     /locus_tag="VD737_10175"
     CDS             complement(2161..2574)
                     /gene="rplP"
                     /locus_tag="VD737_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003448731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HXW10975.1"
                     /translation="MMQPKRTKFRKQFKGNLKGSSHRGSSVAFGDFGLKAVERARMTA
                     REIEAARRAMTRYVKRGGQIWIRVFPDVPITQKPLEVRMGSGKGNVEYWVAKVTPGKV
                     LFEIEGVPEATAREAFKLAAAKLSVSTAFVRRQVR"
BASE COUNT          321 a          941 c          817 g          520 t
ORIGIN      
        1 cggccttgcc ggcgctctgt ccgttgcgga ttcgcgtaag caggtccgcg atcgggtcgt
       61 tcatgctcat gctgcgttcc tcaccagctc gccttgcgca atccgggcac gtcgccgcgc
      121 atcgtcgcct cgcgcagctt cacgcggccg aggccgaact tgctgtagtt gccgcgcgag
      181 cggcccgtga tcgcgcagcg gctgcgggcc cggatcgggc tcgagtcgcg cggcagcttc
      241 tgcagcgcct cgaccgccgc catccggtcc tcctgggagg ccttcgggtc gcggatctgc
      301 tccttgatcc tggcgcgctt cgcggcgtac ttcttcaccg tcttggcccg ccgcttctcg
      361 cgctcgatca tgctggtttt cgccatcggt cacttcctga acgggaaatt gaacgcctcg
      421 agcagcgcgc ggccacgctt gtcgtcgccc gccgaggtcg tgatcgtgat gtccatgccc
      481 cggacctggt cgatctggtc gtacgcgatc tccgggaaga tgatctgctc cttcacgccg
      541 aagctgtagt tgccctggcc gtcgaacgag cgcgggttga cgccgcggaa gtcgcggatg
      601 cggggcatcg ccacgttgat cagccggtcg aggaactcgt acatgcgagc gccgcgcagc
      661 gtcaccatgc agccgatcgg caggttctcg cggatcttga aggtcgcgac cgacttcttc
      721 gacttgcgca ccgaaggctt ctggccggtg atcttcgcca ggtcgccggt ggcggcgtcc
      781 atgaccttct tgtcggccac ggcctcgccc acgcccatgt tgacggtgat cttggtgatg
      841 cgcggcacct gcatgtcgga gacgagcccg agctccgagc ggagcttcgg cttcacgacc
      901 tcgcgatagt gcttctgcag cctggctgat tcggccatct cgatactctc cggttacgcg
      961 tccaccagct cgccgttcga cttgaagaag cgcaccttgc ggccgtcctt gagggtgcgc
     1021 acgccgacgc ggtcggcctt cttcgtcgcc gggttgagca gcgcgacgtt cgacgcgtgc
     1081 agcggggcct ccttctgcac gatgccgccc tgcacgttgc gctgcgggtt cgggcgctgg
     1141 tgcttcttca ccatgttcac gttctcgacc agcacgcggc cgtcggcctg cacgccgacc
     1201 acggttccgg tgcggccctt ctcgcggccc gccgtgacga cgacggagtc gcccttgcgt
     1261 atcttcctca caggacctcc ggcgccagcg agatgatctt catgaacgcg acgctgcgca
     1321 gctcgcgcgt cacgggcccg aagatgcggg tgccgatcgg ctcgagcttg ttagtcagca
     1381 gcacggccgc gttgccgtcg aagcggatca gcgagccgtc cgcgcggcgc acgccgcgcc
     1441 gggtacggac cacgacagcg tcatagacct cgcccttctt gaccttgccg cgcgggatcg
     1501 cgtccttgat gctcaccttg atcacgtcgc ccacgccggc gtagcggcgg cgcgtgccgc
     1561 ccatgacctt gatgcacatc agctcgcgcg cgccggagtt gtccgccgcc tgcagcctgg
     1621 tcctcagctg gatcatggcc ccgtacctcc gctcattccg ccggcgcgcg ctcgagcacg
     1681 cgcaccaggg tccacgactt gttccgcgac agcggccggc actccgagat cgcgaccgta
     1741 tcgccctcgt tgcactcgtt cttctcgtcg tgcgcgagca gcttggtcgt gcgccgcacg
     1801 tacttgccgt aggtcgggtg cttgatcagc cgctcgaccg cgaccgagat ggtcttgtcc
     1861 atcttgttgc tgaccacggt gccggtcagc gtcctcgctt ccttctttgc gctctcggtc
     1921 atcacggctt gctccccgcc tgcgcgcggt ccatctcgtt cagcacggtc ttcacgcgcg
     1981 cgatcttgcc catggccttg gcgtgctggt caggccgcgc cggctggccg gaggccgagg
     2041 ccatgcgcag gttgaactgc tcgcgccgca gctccgcgag ctcctcgcgc agttccgcgc
     2101 gggtcttgcc gcgcagcgcg gtcagcgtcc gggcccgttc cttgacgttc ttcctgctca
     2161 tcagcgcacc tgccgccgta cgaaggccgt ggacaccgac agcttcgccg ccgccagctt
     2221 gaatgcctcg cgcgccgtcg cctccggcac gccttcgatc tcgaacagca ccttgcccgg
     2281 ggtcaccttc gcgacccagt actcgacgtt gcccttgccg ctgcccatgc ggacctcgag
     2341 cggcttctgc gtgatcggca cgtccgggaa cacgcggatc cagatctggc cgccgcgctt
     2401 gacgtagcgc gtcatggcgc gccgcgccgc ctcgatctcg cgcgcggtca tgcgcgcgcg
     2461 ctccacggcc ttgagcccga agtcgccgaa cgcgaccgag ctgccgcggt gggagctgcc
     2521 cttcaggttg cccttgaact gcttgcggaa cttggtccgc tttggctgca tcatgtcctt
     2581 ggtccttcag ctcagcctg
//
LOCUS       DASPFR010000384         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_602055, whole genome shotgun sequence.
ACCESSION   DASPFR010000384 DASPFR010000000
VERSION     DASPFR010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602055"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..158)
                     /locus_tag="VD737_10180"
     tRNA            complement(83..158)
                     /locus_tag="VD737_10180"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(123..125),aa:Ala,seq:cgc)
     gene            233..730
                     /locus_tag="VD737_10185"
     CDS             233..730
                     /locus_tag="VD737_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXW10976.1"
                     /translation="MSSSIRVCDAVGSDLEFLARGNEAMALETEHRTLDAGTVRRGVA
                     TALHDDAHGRYFVAEDESGTAVGQLMVTYEWSDWRNGQFWWIQSVYVVPAARRHGVFK
                     AMYRHVEGLARATAGVCGLRLYVESNNATAQRTYTRCGMHDAHYRVMEVELTGAHLAP
                     LPKEN"
     gene            732..1193
                     /locus_tag="VD737_10190"
     CDS             732..1193
                     /locus_tag="VD737_10190"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXW10977.1"
                     /translation="MLKNGTRAPEFDLEDQHGKRHTLKSLLAGGPLILYFYPADFTPG
                     CTKEACSFRDLQKDLVAARLRVVGVSPQDVASHQRFAEKHGLNFPLLADPDKKAVKAY
                     DVDGPLGFGVRRGTYLIDAEGTIRDSVLADLRIGEHEAFVKRALAAGAGIK"
     gene            complement(1231..1731)
                     /locus_tag="VD737_10195"
     CDS             complement(1231..1731)
                     /locus_tag="VD737_10195"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXW10978.1"
                     /translation="MAAAGVYWLGTWAKTTVVVLVRHAEAGESTSGDPDLSPAGEKRV
                     AALGELLDDLLADRKVDYLYAADTRRSQQTAAPIANQFRLPINVLASSDWSRLAARIR
                     REHRGKTVVVVGYATTLPGVINQLSGQELVIRDDEFDALYLVVMPSPGETRSFRLRYG
                     PQNSPR"
     gene            complement(1628..>2160)
                     /locus_tag="VD737_10200"
     CDS             complement(1628..>2160)
                     /locus_tag="VD737_10200"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HXW10979.1"
                     /translation="AGVELTELAISTSGNYRDFRRLPDGRLVSHTIDPRLGEPVRHAL
                     ASVSVVHPRAQLADAYATALMVLGPDEGYAVAQRLGLPALFLERIGESQQFRERATPE
                     FERLRSPAQQASLSSADGSADQAPAPTFHGAAAVSVARAADGGGRRLLARHLGEDDGG
                     RAGAPRGSRGEHFRRP"
BASE COUNT          379 a          713 c          716 g          352 t
ORIGIN      
        1 cgcggccttg ctgctcgcct tcgccgtgct agccgcctcg gcggcggcgc tcatgatccc
       61 gctgagggac aggtaagaag tttggtggag tggaagggga tcgaaccctc gaccttcgca
      121 ttgcgaacgc gacgctctcc cagctgagct accgccccac acgagggcgc gcattgtagc
      181 accggctctt ggcaccgcaa ccgacgctcg tgtcgacggc tatgatggcg tcatgtcgag
      241 cagcatccgc gtatgcgacg ccgtggggtc ggacctcgag ttcctggcgc gcggtaacga
      301 ggccatggcg ctcgagaccg agcacaggac gctcgacgcc ggcaccgtcc gtcgtggcgt
      361 cgccacggcg ctccacgacg atgcccacgg gcgctacttc gtcgccgagg acgagtcggg
      421 cacagcggtc ggccaactca tggtgacgta cgagtggagc gactggcgca acggccagtt
      481 ctggtggatc cagagcgtgt acgtcgtgcc cgcggcgcgt cgccacggcg tgttcaaggc
      541 catgtatcgc catgtcgaag gcctcgcgcg agccacggca ggcgtgtgcg gtttgcgtct
      601 ctacgtcgaa tcgaacaacg cgacggcaca gcggacctac actcgctgcg gcatgcacga
      661 cgcgcactac cgcgtgatgg aagtcgaact gaccggcgcg cacctggcgc cgctacccaa
      721 ggagaactga catgctcaag aacggcacgc gcgcaccgga gttcgacctc gaggaccagc
      781 acggcaagcg ccatacgctg aagtcgctgc tcgccggcgg gcccttgatc ctctatttct
      841 acccggcgga tttcacgccc gggtgcacga aagaagcctg cagcttccgc gacctgcaga
      901 aggatctggt ggcggcccgt cttcgcgtcg tcggcgtgag tccgcaggac gtcgcgtcgc
      961 accagcgctt tgccgaaaag cacgggctca actttccgct gctggccgac ccggacaaga
     1021 aagccgtgaa ggcatatgac gttgacggac cgctcggatt tggcgtgcgc cgcgggacgt
     1081 acctgatcga cgccgagggg acgatccggg actcggtgct ggcggacctg cgaatcggcg
     1141 agcacgaagc gttcgtcaag cgcgctcttg ccgctggtgc aggtataaaa tagttaaatt
     1201 atcaattcct tatagctact acctagactc ttatctagga ctgttctgcg gtccgtagcg
     1261 tagccggaac gaacgcgtct caccgggact tggcatgacc accaggtaaa gcgcgtcgaa
     1321 ttcgtcgtcg cgaatcacga gctcctgccc gctcaactga ttaatgacgc ctggcagcgt
     1381 cgttgcgtag ccgaccacca cgacggtctt gccgcggtgc tcccggcgga tacgcgcagc
     1441 gagcctcgac cagtcgctgc tggcgagcac gttgatgggc agccggaact ggttcgcgat
     1501 cggcgcggcg gtctgttgcg agcgtcgcgt gtcggcggca tacaggtagt cgaccttgcg
     1561 gtccgccagc aggtcgtcca gcaactcgcc gagcgccgcc acgcgtttct cgcccgccgg
     1621 actcaggtca gggtcgccgg aagtgctctc cccggcttcc gcgtggcgca ccagcacgac
     1681 caccgtcgtc ttcgcccagg tgccgagcca atagacgccg gccgccgcca tcagcagcgc
     1741 gagcaacgga aacagcagcg gcgccatgaa aggtcggcgc cggagcctgg tcagcggatc
     1801 catccgccga tgatagcgaa gcctgttgcg ccgggctgcg cagccgctcg aattcgggcg
     1861 tggcgcgctc ccggaactgc tgcgactcgc cgatgcgttc gaggaacagc gcaggcagtc
     1921 cgaggcgctg cgcgacggca tatccctcgt ctggcccgag caccatcaac gccgtcgcat
     1981 aggcgtcggc gagctgcgcg cgtgggtgca cgacggagac cgaagcaagc gcgtggcgca
     2041 cgggctcacc gagccgcggg tcgatcgtgt gcgacaccag gcgtccgtcg gggagccgcc
     2101 ggaagtcgcg atagttgccc gacgtcgaaa tcgcgagctc ggtcagttcg acgcccgcgt
//
LOCUS       DASPFR010000385         4933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1822728, whole genome shotgun sequence.
ACCESSION   DASPFR010000385 DASPFR010000000
VERSION     DASPFR010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822728"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1579)
                     /locus_tag="VD737_10205"
     CDS             complement(77..1579)
                     /locus_tag="VD737_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10980.1"
                     /translation="MFRSFYLAGFECATGYNMHGEWIDQIAATEHDLHADADYRRLAD
                     VGIRAVREAIRWPLVDRAGRYDFSSVEPFVDAALTHGFDVIWDLFHYGYPDELDPFSA
                     EFAARFGDYCRAAARFVVERMPGQCYFTPMNEPSYFAWAGGDVGRFAPHARGRAAELK
                     VSLARAAIRGVDAIREVCPKSRIVNVDPICRVVVDDDDPARLEHARRFNEHYVFEFWD
                     IVSGRRMPELGGSPSHLDIVGINYYWTNQWALGREGVPLAEDDPRRVPLADLVRSAWR
                     RYGCEIVITETSALGEARAPWIHELSLMAEELLGEGVPLGGICLYPILGMPEWHARDQ
                     WTRMGLWDLEREQDVLERKACGPMMAALRTVQAARGTTLDQRARRLRLPRTGDAPFEA
                     HGRLVWQKIRPQAHALRVFHADRAHGGTWVLATTLRVDGRTVEDVRTASDLSSLAQMA
                     ETDPPAQILQRHGVDPSNWTDAHLEAWRADLRQLSRNLVDFERPLRCPAV"
     gene            complement(1569..2879)
                     /locus_tag="VD737_10210"
     CDS             complement(1569..2879)
                     /locus_tag="VD737_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HXW10981.1"
                     /translation="MHVTRDTLDSLLEPSTFQWLAGIEDTFITAPSARTGRTLDEYEL
                     TGHYERMDEDLALFAELGLRAVRYGIPWHRINPRRGEWDFGWADRPLERLLDLGIAPV
                     VDLVHYGLPGWIDGAYLHPDYPEYVAEFASRIAERYRGRIHTYTPLNEPRVTAWYCGR
                     LGWWPPFGRSWRGFVQVMMGVCRGVVRTVEALRAVDPQIVDVHVDATDLYEAVSAELT
                     HEAARRQEIVFLALDLVSGRVRPGHSLFDWLLENGASAADLDWFATRAVQPDLIGINL
                     YPLFSEKRLVSSKRGLRVRMRYASANIVDRLADLYWSRYRRPLLISETASEGSVARRQ
                     AWLEQSVRATARVRGRGVPLVGYTWWPLFALVTWGYREGRKPAHDYLRQMGLWDLRAD
                     DEGRLARVPTVLVDRYRELVAGGAGAVGRLGEAVADAHGGARVS"
     gene            complement(2882..3949)
                     /locus_tag="VD737_10215"
     CDS             complement(2882..3949)
                     /locus_tag="VD737_10215"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HXW10982.1"
                     /translation="MPPAGRDDSMDTAASPAYVNPVYAGYLADPFVWRCGATYYAVGT
                     GPAEAAGVAASACAPTVFPLLRSADLVHWTESGHALVRPDASLGNAFWAPEVVESEGR
                     WYLYYSVGHDDRLHQLRVAVSERPTGPYVDSGALTSPDEVAFAIDPHPFRDVDGRWYL
                     FHARDFLDPMDEAGREARCGTALVVTPLRDMTTLGDGVRTVARARCDWQRYAAHRTMY
                     GRQFDWHTLEGPCVVRTVEGRYYCLYSGGCWQTDTYGVDYVVAPSIDGPWSDDGSEQG
                     PRVLRTVPGRVVGPGHCSVVHTADGTPVLAYHAWDPDMTARRLCIDVLEFGPDGPRAC
                     GPSWTARPAPLAVGRSQPEEA"
     gene            complement(4084..>4933)
                     /locus_tag="VD737_10220"
     CDS             complement(4084..>4933)
                     /locus_tag="VD737_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016144017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXW10983.1"
                     /translation="LVAHGTPGQQRRWLQPLAAGEMVGAFALTEPQAGSDAANLRTTA
                     RRVEGGYLLNGTKQFITSGAVAGVAITFAVTDADAGKRGMSAFLVPTDAAGFRVGRLE
                     SKLGQRASDTAQLVYEDVFVGDDLRIGDEGEGYRIALANLETGRIGIGAQSVGMAAAA
                     LEQALEYARERTAFGKPLTQHQAVSFRLADMATDVEAARQLVLHAAALKDAGQPCLKE
                     ACMAKLFASERAERVASAAVQVLGGYGYVDDYLVEKIYRDVRVCQIYEGTSDIQRLLI
                     GRELAS"
BASE COUNT          741 a         1740 c         1744 g          708 t
ORIGIN      
        1 ggtgccggcg tacaggggtt gatgcctgcg ggcattcgag accacctcag acggtggtag
       61 acctacacgc gccgttctag acggccggac acctgagcgg gcgctcgaaa tcgacgagat
      121 tccgtgacaa ttggcgcaga tcagcgcgcc aggcttccag atgcgcatcg gtccagtttg
      181 acgggtcaac gccgtgtcgt tgcaggatct gcgccggcgg atccgtctcc gccatctgcg
      241 ccagcgacga gagatcgctc gcggtccgga cgtcttcgac tgtccgcccg tcgacccgca
      301 gcgtggtcgc cagcacccag gtgccgccgt gggcacgatc cgcgtggaac acgcgaaggg
      361 cgtgcgcctg cgggcggatc ttttgccaga ccagccggcc gtgcgcctcg aacggcgcgt
      421 ccccggtgcg cggcaggcgc aggcgtcgtg cgcgctggtc gagcgtggtg ccgcgcgccg
      481 cctgcaccgt gcgcagggcg gccatcatcg gcccgcaggc cttgcgctcg aggacgtcct
      541 gttcacgctc caggtcccac agccccatgc gcgtccactg gtcacgcgcg tgccactcgg
      601 gcatcccgag gatcgggtac aggcagatgc cgccgagcgg cacgccttct ccgagcagtt
      661 cctccgccat cagcgacagt tcgtggatcc acggcgcgcg cgcttcgccg agcgcgctcg
      721 tctcggtgat cacgatttcg cagccgtacc ggcgccacgc gctgcggacg aggtcggcga
      781 gcgggacgcg tcgtggatcg tcttcggcca gcggcacgcc ttcgcggccg agcgcccact
      841 ggttggtcca gtagtaattg atccccacga tgtcgaggtg cgacggtgac ccgccgagtt
      901 cgggcatgcg ccggccgctg acgatgtccc agaactcgaa tacgtagtgt tcgttgaacc
      961 gccgggcgtg ctccaggcgc gccggatcgt cgtcgtcgac gacgacgcgg cagatcgggt
     1021 cgacgttgac gatccgagac ttcggacaga cttcgcggat cgcgtcgacg ccgcgaatcg
     1081 cggcccgggc gagcgagacc ttgagctcgg cggcccggcc gcgcgcgtgc ggtgcgaagc
     1141 ggccgacgtc gccgccggcc catgcgaagt acgagggctc gttcatgggc gtgaaatagc
     1201 actggcccgg catgcgctcg acgacgaagc gcgccgccgc gcgacagtag tcgccgaatc
     1261 gcgcggcgaa ctcggcggag aacgggtcga gctcgtccgg gtacccgtag tggaacaggt
     1321 cccagatcac gtcgaagccg tgcgtcagcg ccgcgtcgac gaacggctcc acggacgaaa
     1381 aatcgtaccg gcccgcgcgg tcgaccagcg gccagcggat cgcctcgcgc accgcccgga
     1441 tgccgacgtc ggcgagacgc cggtagtcgg cgtcggcgtg caggtcgtgc tcggtcgcgg
     1501 cgatctggtc gatccactcg ccgtgcatgt tgtagcccgt cgcgcactcg aacccggcga
     1561 ggtagaagct acgaaacacg ggcacctccg tgcgcgtcgg ccaccgcctc gccgaggcga
     1621 ccgaccgcgc cggccccgcc ggcgacgagc tcgcggtacc ggtccaccag cacggtgggt
     1681 acgcgcgcga ggcgtccctc gtcgtcggcg cgcaggtccc agagtcccat ctgccgcagg
     1741 tagtcgtgcg ccggcttgcg tccttcgcga tagccccacg tcacgagcgc gaacagtggc
     1801 caccacgtgt agccgacgag cgggacgcca cgaccgcgga cgcgggccgt cgcgcgcacc
     1861 gactgctcga gccacgcctg ccgccgggcc accgagccct cggatgcggt ctcgctgatc
     1921 agcagcggtc gccgatagcg cgaccagtag aggtcggcga ggcggtcgac gatgttcgcg
     1981 ctcgcgtagc gcatgcggac gcgcagtccg cgcttcgagg acaccaggcg tttctccgag
     2041 aacagcgggt acaggttgat gccgatcagg tcgggctgca ccgcgcgcgt cgcgaaccag
     2101 tcgaggtcgg cggcgctggc gccgttctcg agcagccagt cgaacagcga gtgaccggga
     2161 cggacgcggc cgctgaccag gtcgagcgcc aggaacacga tctcctgccg ccgggcggct
     2221 tcgtgcgtca gctccgccga gaccgcttcg tacaggtcag tggcgtcgac gtgcacgtcc
     2281 acgatttgcg gatcgaccgc gcgcagcgcc tcgaccgtgc ggaccacgcc gcgacagacg
     2341 cccatcatca cctgcacgaa gccgcgccag ctgcgtccga acggaggcca ccacccgagg
     2401 cggccgcagt accaggccgt cacgcgcggc tcgttgagcg gcgtgtacgt gtgtatgcgt
     2461 ccgcgataac gctcggcgat ccgcgacgcg aactcggcca cgtactccgg gtagtcgggg
     2521 tgcaggtacg cgccgtcgat ccacccgggc aggccgtagt ggaccaggtc gacgacgggc
     2581 gcgatcccga ggtcgagcag gcgctcgagc gggcggtccg cccagccgaa gtcccactct
     2641 ccgcgccgcg gattgatgcg gtgccagggg atgccatacc gcaccgcgcg caggccgagc
     2701 tcggcgaaca gcgcgaggtc ctcgtccatg cgctcgtagt ggccggtcag ttcgtactcg
     2761 tcgagcgtgc gcccggtgcg cgccgagggt gccgtgatga acgtgtcctc gatgccggcg
     2821 agccactgga acgtcgacgg ttcgagcagc gagtcgagcg tgtcccgcgt gacgtgcacg
     2881 atcaggcttc ctcgggctgc gagcggccga ccgcgagcgg ggccgggcgt gccgtccacg
     2941 acggaccgca tgcgcgcgga ccgtccggcc cgaactccag cacgtcgatg cacaagcggc
     3001 gcgcggtcat gtcggggtcc cacgcgtggt acgcgagcac gggcgtcccg tccgcggtgt
     3061 gcacgaccga gcaatggccc ggcccgacca cccggcccgg caccgtgcgc agcacgcgcg
     3121 gtccctgttc cgagccgtca tccgaccacg gcccgtcgat cgacggcgcc acgacgtaat
     3181 cgacgccgta cgtgtcggtt tgccagcaac cgccgctgta gagacagtag tagcgtcctt
     3241 ccacggtgcg gacgacgcac ggaccttcga gcgtgtgcca gtcgaactgg cgtccgtaca
     3301 tcgtccggtg ggcggcgtaa cgctgccagt cgcagcgcgc gcgcgccacg gtgcggacac
     3361 cgtctcccag cgtggtcatg tcacgcagcg gagtcacgac gagcgcggtg ccgcaccgcg
     3421 cctcgcgccc ggcctcgtcc atcggatcca ggaagtcgcg ggcgtggaac aggtaccagc
     3481 ggccgtcgac gtcgcgaaac gggtgcgggt cgattgcgaa cgcgacttcg tccggtgagg
     3541 tgagtgcacc cgaatcgacg tacggccccg tgggccgctc gctcacggcg acgcgcagct
     3601 gatgcaggcg gtcgtcgtgg cccacggagt aatagaggta ccagcggcct tcggactcga
     3661 cgacctccgg cgcccagaac gcgttgccga gcgaagcgtc ggggcgcacc agcgcgtgac
     3721 ccgactccgt ccagtggacg aggtccgctg accgcagcag cggaaacacc gtcggtgcgc
     3781 acgcgctggc ggccacgccg gccgcctcgg cgggaccggt gccgaccgcg tagtacgtgg
     3841 cgccgcagcg ccagacgaac gggtcggcga ggtatccggc gtaaacggga ttgacgtaag
     3901 cgggagaggc ggccgtgtcc atgctgtcgt cgcggcctgc gggcggcacg aaagcagcca
     3961 ccttcgacgt cgcggcgcgc gctggctata ggcgcatgct gtggggagcc gtcatcttcg
     4021 gtcgcacggc ggcccgtgca atatttactt tccctggatc agggtcgcga ctcgccggtc
     4081 cgctcagctc gcgagttcgc gaccgatcag caggcgctgg atgtcggagg tgccttcgta
     4141 gatctggcag acccgcacgt cgcggtagat cttctcgacc aggtagtcgt cgacgtaacc
     4201 gtacccgccg agcacctgca ccgcggccga ggccacgcgt tcggcgcgct cggacgcgaa
     4261 cagcttcgcc atgcacgcct ccttgaggca cggctggccc gcgtccttga gcgcagcggc
     4321 gtggagtacc agctgtcgcg ccgcctcgac gtcggtcgcc atgtccgcca ggcggaacga
     4381 caccgcctgg tgctgggtca gcggcttgcc gaacgccgtg cgctcgcgcg cgtactcgag
     4441 cgcctgctcg agcgcagcgg cggccatgcc gacgctctgc gcgccgatgc cgatccggcc
     4501 ggtctcgagg ttggcgagcg cgatccgata gccttcgccc tcgtcgccga tccgcaggtc
     4561 gtcgccgacg aacacgtcct cgtagaccag ctgcgccgtg tccgaggccc gctggccgag
     4621 cttcgactcg agccggccca cgcgaaagcc cgccgcgtcc gtcggcacga ggaacgcgct
     4681 catgccgcgc ttgccggcgt cggcgtccgt caccgcgaac gtgatcgcga cgcccgcgac
     4741 cgcgccggag gtgatgaact gcttggtgcc gttcagcagg taaccgccct cgacgcgccg
     4801 cgcggtggtg cgcaggttgg cggcatcgga acctgcctgc ggttcggtca gtgcaaaggc
     4861 gccgaccatt tcgccggcgg cgagcggctg cagccagcgc cgctgctgcc ccggcgtgcc
     4921 gtgggcgacg agg
//
LOCUS       DASPFR010000386        10205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_602444, whole genome shotgun sequence.
ACCESSION   DASPFR010000386 DASPFR010000000
VERSION     DASPFR010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10205
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602444"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..688)
                     /locus_tag="VD737_10225"
     CDS             complement(14..688)
                     /locus_tag="VD737_10225"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HXW10984.1"
                     /translation="MSLELSALEARVIGCLMEKQIATPDQYPLSLNALVNACNQRSNR
                     DPVLNLEEREVQAVVDVLMRKQLVLEKSGFGSRVPKYHQLFCNTQFGSLKFSPPQTAI
                     VCELLLRGPQTPGELRTHAARLAPIRDVEEVETALEDLMTRPEGPYVVKLAREPGRRE
                     SRYAQLFTGPPATPLAPEHGPEAATPPSAASGDGALVARIETLESTVAMLQRELAELR
                     ERLSHP"
     gene            760..1662
                     /locus_tag="VD737_10230"
     CDS             760..1662
                     /locus_tag="VD737_10230"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF010621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HXW10985.1"
                     /translation="MQSPPKRGSTLKGVVTLSLMVANVVFWVPLLLLVALARLVVPLR
                     QSRRLMARWMTALAAAWITCNGAIFRLMGVLRLQVEGLAGLAPREWYLVVSNHRSWVD
                     ILVLQEVFNQRIPFLKFFLKRELIWVPFLGLAWWALEFPFMKRHSSAYLARHPEARGQ
                     DLAATRKACAKFAQIPTSVMNFVEGTRFTPQKQAAMGSPYRHLLLPRAGGVAFVLSAM
                     GQMLHSMLDVTIAYRESSPTLWDLCCGRLETVRVHVLKRPIERWTTEGDYGDDAAFRA
                     RFQQWLGEIWNEKDRRLAAMSVAS"
     gene            1659..2147
                     /locus_tag="VD737_10235"
     CDS             1659..2147
                     /locus_tag="VD737_10235"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HXW10986.1"
                     /translation="MSWDLPSPFVHRRVAQHAEIDGYGHVNNAIYVAWLDDCAWAHSI
                     DRGISPELCRSLNRGMAVWRTQLHYLRSALEGDAIEVATWPVHNDNKLRIERRFQVRR
                     AADGETLVRALIHYVCIDLQTGKAKRMPAEFTQYTVPPEVAAAIAAETAPYEPGVEPR
                     AR"
     gene            2209..3090
                     /locus_tag="VD737_10240"
     CDS             2209..3090
                     /locus_tag="VD737_10240"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXW10987.1"
                     /translation="MHDQWTNADLASLLGSTPSLTRRDILVTSLAAGFALAVQPVSAD
                     TITTGTEGLTAGEVRIPVKDGEIPGYRAMPAKGENFPVVLVVQEIFGVHEHIRDLCRR
                     FAKQGYLAVAPELYARQGDVSKITDWKQIISEVVAKVPDEQVMADLDATVAWAGKSSK
                     GDTRRVAVTGFCWGGRIVWLYAAHSKDLKAGVAWYGRLDGDRTPLQPKYPVDVAAQLH
                     APVLGLYGGQDQGIPLSDVDRMRAALEKAGEPSKIVVFPDAPHGFLADYRPSYSPKEA
                     ADGWAQCLAWVREHGVG"
     gene            complement(3113..5419)
                     /locus_tag="VD737_10245"
     CDS             complement(3113..5419)
                     /locus_tag="VD737_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXW10988.1"
                     /translation="MSAPPARRPLVPVARPRRACVTLATLVLCGFVAAGHAAPPFRGR
                     SVEAVIRELADDGGLQLVYTRDLVPTELVVQQEPTATAPVAAIGQVLASSGLALRQVD
                     GRTYAVVRVARAATAKPESAASALDEVVVTSSRYSLAADVPDVHTLLTQDELVALPRL
                     AEDTLKAVHRLPGAASNGLAGLAHLRGGEANETLVLLDGLPLYEPFHLRLLQSPASVL
                     DERIVEGLDAYAGGFTADFGDRMSGIIDARSRHPEADAYYELGLSLMHANALASRSFA
                     AGRGRWLASFRRSNLDEIADTLQSELGEPTYADGFARLDYAWSPSTRGSLHLLLASDE
                     AQINNAAETESSDAEYSNSYLWGTLAHDWTPRLTTTALLSYTDVSARRVATVDEPGLR
                     SGAAYDERDYDVLGLRLDAKYAAGRTLHRAGLDLRSLEARYQYRGEVTSAPGYPFPGA
                     SDFVRELSPAPGGEHVALYYTVRAQLTDALTGELGLRWDEQTYGPDSDDQLGPRVNLA
                     WRLDERTRLLASWGRHQQFQGIEELQVEDGVEEFQRAQRAEHAILGLERELGPDVSLR
                     VEAYRKDYTRLRTRYENLYDPLSLAPELRWDRVAVDPASARADGVEVLLTHRPAGDWS
                     GWASYAWSRVVDRLDGADVRRSWDQTHTLNAGFGWMHGPWQATVAAQYHTGWPVTPIG
                     LDATGSVEVGARNDHRYAHFGSLDARLSHDWTLAHGTLTAHAEVTNALDRRNPCCTDL
                     AYGADGTLTRDLRHWLPLVPSIGVLWKF"
     gene            complement(5478..6506)
                     /locus_tag="VD737_10250"
     CDS             complement(5478..6506)
                     /locus_tag="VD737_10250"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HXW10989.1"
                     /translation="MTDERTDPATDHDDDADLRALLQSSGARPVPPAEMTDEVRAAVA
                     AEWRSVVAARRPQRRVVPWLAAASAAAVAVGAWFALTQPVGPGAVVATVARLDGAAEV
                     RHGSATSWESIAAGADLRAGDRVRTPATGRLALRRTDGLEVRLDVDTAVAFEDDERAR
                     LEAGRVYIDSGRTDARTDAFVVETALGAVRHLGTQYAVSVARASMAVAVREGNVAIDH
                     GGGHALASAGESVTLAADGRLERAALAPHDAAWGWAEAAAPAFAIEGRTLDEFLTWAA
                     RETGRRLVYASAAAAREAQQTLLRGSVDGLAPEEAIGAVLMTTPALKARVGGAQLRVE
                     RALPPAAP"
     gene            complement(6518..7129)
                     /locus_tag="VD737_10255"
     CDS             complement(6518..7129)
                     /locus_tag="VD737_10255"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXW10990.1"
                     /translation="MNDDRDLVAAMLAGSEHAFTVFFDIYFPRVYRFALPRLDRNPDV
                     TKDVVQATLIKAMRAIDGWRGEAALFTWLCQICRRQIADHVRSQRRHSGRVVLIDDSD
                     EVRAALESIEAPESDDPFRGADSAELRRLVHAVLDRLPSRYGEALEWKYVEGHTVEEI
                     GQRLGIGQVAAQSLLARARIAFREGLEAVFGASARDVLASLGS"
     gene            7351..8022
                     /locus_tag="VD737_10260"
     CDS             7351..8022
                     /locus_tag="VD737_10260"
                     /inference="COORDINATES: protein motif:HMM:NF023682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermostable hemolysin"
                     /protein_id="HXW10991.1"
                     /translation="MNVPHHVDRTAERGHRQHEPAVRPPPGHGLRLEAVAPEDASRAE
                     LERYVQAAFLAKHGAHVRSFMPTLLAFRDRAGALGGVAGIRGGDEGRLYLEQYLDRPV
                     EQALSNALAARGIGRVERGQIAEVGNLAGASCRSAVRMVAQIPAYLLSRRYAWIVFTA
                     TSALRGILARFDAPLVELARADGLRVTGTHDEWGRYYETDPRVYAGWLRDAERLPGFA
                     HTGAR"
     gene            8024..9502
                     /locus_tag="VD737_10265"
     CDS             8024..9502
                     /locus_tag="VD737_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXW10992.1"
                     /translation="MLLQAIARAEAGVVALDDGVRAITYGDLGELLRLERDWLRAGRG
                     ERYALAADNGVAWALADLALHTGAWPSVPVPGAFTETLKRHALDDAGIDTLLTDDDTA
                     MRALLPDWTSDGVAPASGLHRFRRSPDACDKANLPAGTRKITYTSGSTARPKGVCLDG
                     AALEAVAGSVAEATSELPIARHLCLLPLWTLLENVAGLYAPLLRGATCVLPSCATTGM
                     GHADLDARRLLGTISAQSPHSLILVPELLQVLVEAAERGWRVPRSLRFVAVGGARVTT
                     SLLERAEAVRLPVYEGYGLSECASVVCLNTPRARRAGTVGRPLPHVRVRVDGDGQVRV
                     AGHVMLGYLGDRPPPGGELATGDLGTFDDDGYLRLTGRAGHRFITSYGRNVSPEWIES
                     EISQRLGAAPVCAFGEARPYVVALVGASHAAANDAAIERAIADANSVLPDYARVRRWA
                     RADRPFSFDDGTLSANGRLRRAEIRARHDALVQALYTEAIAS"
     gene            9526..>10205
                     /locus_tag="VD737_10270"
     CDS             9526..>10205
                     /locus_tag="VD737_10270"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HXW10993.1"
                     /translation="MHLYERLQAETAADRARLLAAPPIQRALRGDVTRELYVEFLTQA
                     YHHVRHTVPLMMAAGGRLSERQGWAREPLLHYLEEEAGHEQWILDDIAHAGGDRELAA
                     ASLPSLATEAMVAYAYDTVMRRNALGFFGMVYVLEGTSVALALEAADRIRATLQLPVH
                     AFSYLRSHGELDRQHVGDLAGVLARIDDEADATAVIRCARGVFWLYGEMFRMLEAVVE
                     SHDASPAR"
BASE COUNT         1462 a         3631 c         3579 g         1533 t
ORIGIN      
        1 agtgtcccca ttttcacggg tgtgacaggc gctcccggag ctccgccaac tcgcgctgca
       61 gcatcgccac cgtcgactcg agcgtttcga tgcgggcgac cagcgcaccg tcgcccgacg
      121 cggccgacgg cggcgtggca gcctccggac cgtgttcggg agccagcggc gtcgcgggcg
      181 gccccgtgaa gagctgcgca tagcgcgact cgcggcgtcc cggttcgcgc gccagcttga
      241 cgacgtacgg gccttccgga cgcgtcatga ggtcctcgag cgcggtctcg acctcctcga
      301 cgtcgcggat cggcgcgagc cgcgcggcgt gcgtgcgcag ctcgcccggc gtctgcggtc
      361 cgcgcagcag cagctcgcag acgatggccg tctgcggcgg cgagaacttg agcgacccga
      421 actgcgtgtt gcagaagagc tggtggtact tgggcacgcg gctgccgaag cccgacttct
      481 cgagcaccag ttgcttgcgc atcaggacgt cgaccacggc ctgcacctcg cgctcctcga
      541 ggttgagcac ggggtcgcgg ttgctgcgct ggttgcaggc gttgacgagc gcgttcagcg
      601 acaacggata ctggtccggc gtcgcgatct gcttctccat caggcaaccg atcacgcggg
      661 cctcgagtgc cgacagctcg agcgacattg gctggcctcc ccagcgcgtc cgacccgggc
      721 ataatctcac acccgcagcc gatcgagggc aggccgaccg tgcagtctcc gccgaagcgt
      781 ggctcgacgc tcaagggggt cgtgaccctc tcgctgatgg tggccaacgt cgtcttctgg
      841 gtgccgctgc tgctgctggt ggcgctcgcc aggctcgtcg tgccactgcg ccagtcgcgc
      901 aggctcatgg cccgctggat gacggcactc gccgcggcct ggatcacgtg caacggcgcg
      961 atcttccgcc tgatgggcgt gctgcggctc caggtcgagg gcctcgccgg gctcgcgccg
     1021 cgcgaatggt acctggtcgt gtcgaatcac cgctcgtggg tcgacatcct ggtgctgcag
     1081 gaagtgttca accaacgcat cccgttcctg aagttcttcc tgaagcgcga gctgatctgg
     1141 gtgccgttcc tcggcctcgc ctggtgggcg ctcgagtttc cgttcatgaa gcgccactct
     1201 tcggcctatc tcgcgagaca cccggaagcg cgcgggcagg atctcgcggc gacgcgcaag
     1261 gcctgcgcca agtttgcgca gatcccgacc tcggtgatga acttcgtcga gggcacacgc
     1321 ttcacgccgc agaagcaggc cgccatgggt tcgccgtacc ggcacctgct gctgccacgc
     1381 gccggcgggg tcgccttcgt gctgtccgcg atgggccaga tgttgcactc catgctcgac
     1441 gtcacgatcg cgtaccgcga gtcgagcccg acgctctggg acctctgctg cggccgtctc
     1501 gagacggtgc gcgtgcacgt gctcaagcgc ccgatcgagc gctggaccac cgagggcgac
     1561 tacggcgacg acgcggcatt tcgcgcgcgc ttccagcagt ggctcggcga gatctggaac
     1621 gaaaaggatc ggcgcctcgc cgcgatgtcg gtggcctcgt gagctgggac ctgccgtcgc
     1681 cgttcgtcca tcgccgcgtc gcgcagcatg cggagatcga cggctacgga cacgtcaaca
     1741 acgcgatcta cgtcgcgtgg ctcgatgact gcgcgtgggc gcactcgatc gatcgcggca
     1801 tcagccccga gctctgtcgc tcgctcaacc gcggcatggc ggtctggcgc acgcagctgc
     1861 actacctgcg ctcggcgctt gagggcgacg cgatcgaggt cgcgacctgg cccgtccaca
     1921 acgacaacaa gctgcggatc gagcgtcgat ttcaggtgcg gcgagctgcg gacggcgaga
     1981 cgctcgtgcg cgcgctgatc cactacgtct gcatcgacct gcagaccggc aaggcgaagc
     2041 gcatgccggc ggagttcacg cagtacacgg tgccgcccga ggtggcggcg gcgattgccg
     2101 cggagaccgc gccctacgag ccgggcgtcg aaccacgcgc cagataatca ggagtgtccc
     2161 cagttctggg gaaatccgga gtgtccccat tttcacggag ggaagcagat gcacgaccaa
     2221 tggaccaacg ccgacctggc ttcgctgctc ggcagcacgc cgtcgctgac gcgccgcgac
     2281 atcctcgtca cttcgctggc ggccggtttc gcgctcgccg tgcagcccgt gtccgcggac
     2341 acgatcacga ccggcacgga ggggctcacg gccggagaag tgcgcatccc ggtcaaggac
     2401 ggcgagatcc cgggctaccg cgcgatgccg gcgaagggcg agaactttcc cgtcgtgctc
     2461 gtcgtgcagg aaatcttcgg cgtgcacgag cacatccgcg acctgtgccg gcgtttcgcc
     2521 aagcagggct acctggccgt ggcgcctgag ctctacgcgc gccagggcga tgtctcgaag
     2581 atcaccgact ggaagcagat catttccgag gtcgtcgcca aggtccccga cgaacaggtc
     2641 atggccgacc tcgacgcgac cgtcgcctgg gccggcaagt cgagcaaggg cgacaccagg
     2701 cgtgtcgcgg tcaccggctt ctgctggggc ggtcgcatcg tctggctcta cgccgcgcac
     2761 agcaaggacc tcaaggctgg cgtcgcctgg tacgggcggc tggacggcga tcgcacgcca
     2821 ctgcagccga aatatccggt ggacgtcgcg gcgcagttgc acgcgccggt actgggcctc
     2881 tacgggggcc aggaccaggg catcccgctc agcgacgtag acaggatgcg cgcggcactc
     2941 gagaaggcgg gcgagccgtc gaagatcgtc gtgttccccg acgcgccgca cggcttcctc
     3001 gccgattacc ggccgagcta cagcccgaag gaagccgcgg acggctgggc acagtgcctg
     3061 gcgtgggtcc gggagcacgg cgtcggctga gcgtgcggac atcctgcgtc gatcagaact
     3121 tccagagcac tccgatcgaa ggcaccagtg gcagccagtg gcgcaggtcg cgagtgagcg
     3181 tgccgtccgc gccgtaggcg aggtccgtgc agcacgggtt gcggcggtcg agcgcgttgg
     3241 tgacttcggc gtgcgccgtg agcgtgccgt gcgccagcgt ccagtcgtgg ctcaatcgcg
     3301 cgtcgagcga gccgaagtgc gcgtagcgat gatcgttgcg cgcaccgacc tccacgctcc
     3361 cggtcgcgtc caggccgatc ggcgtcactg gccacccggt gtggtattgc gcggcgaccg
     3421 tcgcctgcca gggtccgtgc atccacccga agccggcgtt gagtgtgtgc gtctggtccc
     3481 agctgcggcg cacgtccgcg ccgtccagcc ggtccacgac tcgcgaccac gcgtacgacg
     3541 cccagccact ccagtcgcct gccgggcggt gcgtgagcaa cacctcgacg ccgtccgcgc
     3601 gcgccgacgc ggggtccacg gcgacgcggt cccagcgcag ttccggcgcg agtgacagcg
     3661 gatcgtagag gttctcgtaa cgcgtccgca gccgcgtgta gtccttgcga taagcctcga
     3721 cgcgcagcga gacgtcgggg ccgagttcgc gctcgaggcc caggatcgcg tgctcggcgc
     3781 gctgtgcacg ctggaactcc tcgacgccgt cttccacctg cagttcctcg atgccctgga
     3841 actgctggtg tcggccccag ctcgccagca ggcgcgtccg ctcgtcgagg cgccaggcca
     3901 ggttcacgcg cgggccgagc tggtcgtccg aatcgggtcc gtacgtctgc tcgtcccagc
     3961 gcaatccaag ttcgccggtc agcgcgtcgg tcaactgcgc gcgcaccgtg taatacagtg
     4021 ccacgtgctc gccgcccggg gcgggagaca gttcccggac gaagtccgac gcgcccggaa
     4081 aaggatagcc gggcgcagac gtcacctcgc cgcggtactg gtaccgggcc tcgagcgagc
     4141 gcaggtcgag accggcacga tgcaaggtgc gcccggccgc gtatttcgcg tcgagccgca
     4201 gtccgagcac gtcgtagtcg cgttcgtcgt acgcggcgcc gctgcgcagg ccgggttcgt
     4261 cgacggtcgc gactcgccgc gccgagacgt ccgtgtacga cagtagcgcc gtggtcgtga
     4321 gtcgcggcgt ccagtcgtgc gcgagcgtgc cccagaggta gctgttcgag tactcggcgt
     4381 cgctcgactc ggtctccgcg gcgttgttga tctgcgcctc gtcgctcgcg agcagcaggt
     4441 gcaggctgcc gcgcgtcgag ggcgaccacg cgtaatccag ccgggcaaag ccgtccgcgt
     4501 aggtcggttc accgagctcc gactgcagcg tgtcggcgat ctcgtcgagg ttgctgcgcc
     4561 ggaacgaggc cagccaccgc ccacggcccg ccgcgaacga acgcgaagcg agcgcgttcg
     4621 cgtgcatcag gctcaagccc agttcgtagt acgcatccgc ctccgggtga cgcgaccgcg
     4681 cgtcgatgat cccgctcatg cgatcgccga agtcggccgt gaacccgccc gcgtatgcgt
     4741 cgagaccctc gacgatgcgc tcgtcgagca cgctcgccgg gctctgcagc agtcgcaggt
     4801 ggaacggctc gtacagcggc aggccgtcca gcagcacgag cgtctcgttg gcctcgccgc
     4861 cgcgcaggtg cgcgagcccg gcgaggccgt tgctggccgc acccggcagg cgatgcacgg
     4921 ccttgagcgt gtcttcggcc aggcgtggca gcgccacgag ttcgtcctgt gtcaggagcg
     4981 tgtgcacgtc cggcacgtcg gcggcgagcg agtagcgact cgacgtgacg accacttcgt
     5041 cgagcgcgct cgcggcggac tccggtttcg ccgttgccgc gcgtgcgacg cgcacgaccg
     5101 cgtacgtgcg tccgtcgacc tggcgcagtg ccagtcccga cgacgccagc acctggccga
     5161 tcgcggcgac gggcgcggtt gcggtcggtt cctgctggac gaccagctcg gtgggcacga
     5221 ggtcacgcgt gtagacgagc tgcagtccgc cgtcgtccgc cagttcgcgg atcaccgctt
     5281 cgacggagcg gccgcgaaaa ggcggggcgg catgacccgc agccacgaag ccgcacagca
     5341 cgagggtcgc cagcgtgacg caggcgcgac gaggtcgtgc gaccgggacg agtgggcggc
     5401 gggcgggcgg tgcggacata cgacgcccgc gcggcgcggc gggcggtcgt gatgttagcc
     5461 gagcgcgggc gcagcgacta tggcgcggct ggcggtagag ctcgctcgac gcgaagttgc
     5521 gcaccgccga cgcgggcttt gagcgcgggc gtcgtcatca gcacggcgcc gatggcctct
     5581 tccggcgcga gcccatcgac ggagccccgg agcagtgtct gctgcgcttc gcgggcggcc
     5641 gcggccgacg catagacgag gcgtcggccc gtttcgcgcg cagcccacgt gaggaactcg
     5701 tccagcgtcc gcccttcgat ggcgaacgca ggcgccgcgg cttccgccca gccccacgcg
     5761 gcgtcgtgcg gtgccagcgc ggcacgctcg aggcgtccgt cggcagccaa cgtgaccgac
     5821 tcgcccgcac tcgcgagcgc gtggccgccg ccgtggtcga tcgccacgtt gccttcgcgc
     5881 actgcgaccg ccatggacgc cctcgccacg ctgacggcgt actgcgtccc gaggtgacgc
     5941 accgctccga gcgccgtctc gaccacgaac gcgtctgtgc gcgcatccgt gcggccggag
     6001 tcgatgtaaa cgcgtcccgc ttcgagccgc gcgcgctcgt cgtcttcgaa cgcgaccgcg
     6061 gtgtccacgt cgaggcgaac ctcgagaccg tcggtccggc gcagtgccaa ccggccggtc
     6121 gccggcgtgc gcacgcgatc gccggcacgc aggtcggcac ccgcggcgat cgattcccac
     6181 gacgtggccg atccgtgtcg cacctcggcc gctccgtcca gccgtgccac cgtcgcgacc
     6241 acggcgccgg gcccgacagg ctgggtcaac gcgaaccagg cgccgacggc gaccgccgcc
     6301 gcggacgctg ccgcgagcca cggcacgaca cgtcgctgcg ggcgtcgcgc cgcgacgacc
     6361 gagcgccact ccgcggcgac ggcggcccgc acctcgtcgg tcatctcggc cggcggcacc
     6421 ggccgcgccc cggaggactg cagcagggcc cgcagatctg cgtcgtcgtc gtggtcggtc
     6481 gccggatcag tgcgctcgtc ggtcatggtc acgtccctca ggagcccagg ctcgcgagca
     6541 cgtcgcgagc ggacgccccg aacactgctt cgagtccctc gcgaaacgcg atgcgcgcgc
     6601 gcgcgagcag cgactgcgct gccacctggc cgatgccgag ccgctgcccg atctcctcca
     6661 ccgtgtggcc ttctacgtac ttccactcga gcgcctcgcc gtagcggctg ggcagccggt
     6721 cgagcactgc gtgcacgagg cgacgcagct cggcgctgtc cgcgccacgg aacgggtcgt
     6781 ccgactcggg cgcctcgatg gattccagcg cggcgcggac ttcgtcgctg tcgtcgatca
     6841 gcacgacgcg cccggaatgg cggcgctgcg accgcacgtg atcggcgatc tgccgccggc
     6901 agatctggca cagccaggtg aacagcgccg cctcgccgcg ccagccgtcg atcgcgcgca
     6961 tggccttgat caaggtggcc tgcacgacgt ccttggtgac gtccgggttg cggtcgagcc
     7021 tcggcagggc gaaccggtac acgcggggga aatagatgtc gaagaacacg gtgaacgcgt
     7081 gctcgctgcc cgccagcatc gctgcgacca ggtcgcgatc gtcgttcatg cctgggtcca
     7141 tgtgcgcacg ccagtaggac gcagcgcgcc tcccgaatca atcgccggca ccctcgcagc
     7201 ggcgggccgg cgtgtcttga cggtggtctg acatctcgca agtgattgaa aaaataggct
     7261 ttttatttcg cggtcgccgc ccgattgatt tggccgccgc gccgcgtctc accagagccg
     7321 tgcccgcagc cgggccaccg aggatcctcg ttgaacgtac cgcatcacgt cgaccgaacc
     7381 gccgagcgcg ggcatcgcca gcacgagccg gcggtcaggc cgccgccggg ccacgggctg
     7441 aggctcgagg ccgtggcgcc ggaagacgcg tcccgtgccg aactcgagcg ctacgtccag
     7501 gcggcgttcc tggccaagca cggcgcgcac gtgcggtcat tcatgcccac gctgctcgcg
     7561 ttccgggatc gcgccggggc gctcggtggc gtcgccggca ttcgcggcgg cgacgagggc
     7621 cggttgtacc tcgagcaata cctggatcgt cccgtcgagc aggcgctgtc gaacgcgctc
     7681 gccgcacgcg gcatcgggcg cgtcgagcgg gggcagatcg ccgaagtcgg caacctggcc
     7741 ggcgcgagct gccggtccgc cgtgcgcatg gtcgcgcaga tcccggcgta tctcctgtca
     7801 cgacgctatg cgtggatcgt gttcacggcg accagcgcgt tgcgcggcat cctggcccgc
     7861 ttcgacgctc ccctcgtcga actggcgcgt gccgacggcc tgcgcgtcac cgggacgcac
     7921 gacgaatggg gtcgctatta cgagacggat ccgcgcgtct acgccgggtg gttgcgcgac
     7981 gccgagcgac tgcccggctt tgcgcacacg ggtgcgcgct gacgtgctgc tgcaagcgat
     8041 cgcgcgtgcc gaagccggcg tcgtcgccct ggacgacggc gttcgtgcca tcacatatgg
     8101 cgacctcggt gagctgctcc gcctcgagcg tgactggctg cgcgccggcc gcggcgaacg
     8161 ctacgcactg gccgcggaca acggcgtcgc atgggccctg gccgacctcg ccctgcacac
     8221 gggggcctgg ccgtccgtgc cggtgccggg tgcctttacc gaaactctca agcgtcatgc
     8281 gctcgacgac gcgggcatcg acacgctgtt gaccgacgac gacacggcga tgcgtgcgct
     8341 gctgcccgac tggacgagcg acggcgtggc cccggcctcg ggcctgcacc gctttcgccg
     8401 cagcccggat gcatgcgaca aggcgaacct cccagccggg acgcgcaaga tcacctacac
     8461 gtcgggcagc acggcgcgac cgaagggcgt ctgcctggac ggggccgccc tcgaggccgt
     8521 ggcgggctcg gtggccgagg ccacgtcgga actgccgatc gcgcgccacc tgtgcctgct
     8581 gccgctctgg acactgctcg agaacgtggc ggggctgtat gcgccgctgc tgcgcggcgc
     8641 gacctgtgta ctgccgtcct gcgccacgac gggcatgggc cacgcggacc tcgacgcgcg
     8701 gcggctgctc ggcacgatct cggcgcagtc gccgcacagc ctgatcctcg tgcccgaatt
     8761 gctgcaggtg ctggtcgaag ccgccgagcg gggctggagg gtgccgcggt cgctccgctt
     8821 cgtcgcggtt ggcggcgcac gtgtcaccac gtcgttgctc gaacgcgccg aggcggtgcg
     8881 cctgcccgtg tacgaaggct acggcctcag cgagtgcgcc tcggtggtct gcctcaacac
     8941 gccgcgggcc cggcgcgcgg gcacggtggg ccgcccgctg ccgcacgtgc gggtgcgcgt
     9001 cgacggtgac ggccaggtcc gcgtggccgg ccacgtgatg cttggatacc tcggcgaccg
     9061 accgccgccc ggcggcgaac tcgcgaccgg ggacctcggc acgttcgacg acgacggcta
     9121 ccttcgactg accggccgcg ccggccatcg cttcatcacc agctatggcc gcaacgtctc
     9181 gcccgagtgg atcgagagcg agatctcgca gcgcctcggt gccgcgccgg tctgtgcctt
     9241 cggcgaggca cgaccgtacg tcgtggcgct ggtcggcgcg agtcacgccg cggccaacga
     9301 tgcagcgatc gagcgcgcca tcgcggatgc gaactcggta ttgccggact atgcccgcgt
     9361 gcggcgctgg gcccgcgccg accgcccatt ttctttcgac gacggcacgc tctcggccaa
     9421 cggccgcctg cggcgcgccg agatccgcgc ccgccacgac gcgctcgtcc aggcgctcta
     9481 cacggaggcg atcgcctcct gacgcgacag aggaactccc cgcacatgca cctgtacgaa
     9541 cgactgcagg ccgagacggc cgccgaccgc gcgcgcctgc tggcggcgcc gccgatccag
     9601 cgagcgctgc gcggcgacgt gacccgcgag ctctacgtcg agttcctgac ccaggcttat
     9661 caccacgtgc gccacacggt gccactgatg atggccgccg gcggccgcct gtccgagcgt
     9721 cagggctggg cgcgggagcc gttgctgcac tacctggagg aggaggccgg tcacgagcag
     9781 tggatcctcg acgacatcgc gcacgcaggc ggcgaccgcg agctcgccgc cgcgtcgctg
     9841 ccgtcgctcg ccacggaagc gatggtcgcc tacgcgtacg acaccgtcat gcgccgcaac
     9901 gcgctcggat tcttcggcat ggtctacgtg ctcgagggca cgagcgtcgc actcgcgctc
     9961 gaggcggccg atcgcatccg ggcgacgctg caactgcccg tccacgcatt cagttacctg
    10021 cgcagccacg gcgaactcga ccgtcagcac gtgggcgatc tcgccggcgt gctggcacgc
    10081 atcgacgacg aagccgacgc gaccgcggtg atccgctgcg cgcgaggcgt cttctggctc
    10141 tacggcgaga tgtttcggat gctcgaggcc gtcgtcgagt cgcacgacgc gtcgcccgcg
    10201 aggtt
//
LOCUS       DASPFR010000387         4956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1213616, whole genome shotgun sequence.
ACCESSION   DASPFR010000387 DASPFR010000000
VERSION     DASPFR010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213616"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VD737_10275"
     CDS             complement(<1..756)
                     /locus_tag="VD737_10275"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXW10994.1"
                     /translation="MTATSPATLLSRRFPAKRAFVTGAASGLGFAMAECLARDGWSLG
                     LLDLSTERLEQAVSDLRRSGARDVTGFPGDVASPSVVAGAMGDFVARHGGLDVLVNNA
                     GVAVAGAVELTAVEDWRWIVDINLLGVVWGCRAALPVLKRQGQGLVLNVASSAGFAAA
                     PQMAAYNVTKAGVIALSETLVTELAGTGLQVSVAMPGFFRTHLLDEMRAPPEENRLAH
                     QLMDHSGHDPAEAAIVLLGAAAAGQTWIVWPPEY"
     gene            829..1140
                     /locus_tag="VD737_10280"
     CDS             829..1140
                     /locus_tag="VD737_10280"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HXW10995.1"
                     /translation="MRKVYTGESVIEVAHLRNVLEGAGIRCFVRNEGLAGVIGEIPYV
                     ECWPELWVVRNGDALRARGLIDEARQSASAPGPDWRCERCGELVEGQFDSCWRCAEPR
                     E"
     gene            1169..1927
                     /locus_tag="VD737_10285"
     CDS             1169..1927
                     /locus_tag="VD737_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW10996.1"
                     /translation="MTARTPTGPTTHPATDPTTITIARRFNGPPDSANGGYVCGAFAV
                     ATGEGLRVRLLAPPPLERPLAVRVGDGDGSFWLRDGDQPVAKAVPSQLELEVPAAPPY
                     VQAVWASQHYAGFRQHTFPSCFVCGPHRRRGDGLRIFPGVLDSGIVAAPWLPADDLDG
                     GDGKVAVEFHWAALDCPGYFALVGDRPRKMLLGEMQAHVDRRVHVGESCTVIGWSIGV
                     DGRKHYSGTAIFDEDGELCARARTTWVEVVERGD"
     gene            complement(1963..3213)
                     /locus_tag="VD737_10290"
     CDS             complement(1963..3213)
                     /locus_tag="VD737_10290"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HXW10997.1"
                     /translation="MVTLIVLGVILLLVLVIWSMKRHRDPKLQVECDAPIDQLMPSLA
                     GLSLGTCVAGNSVEVLENGAYFDVLLEEIAAARHSVHFETFLWEEGVLGKRLADAFCE
                     RARAGVSVRVLVDAQGSKKMGKAVEQQLEAAGCKLTKFHPFDLRHIGVFNERDHRKIV
                     VLDGRTAFLGGHCIVDTWLGDAEDDKHFADVSVRLRGPIVHSVQSAFSENWVGQTGEL
                     FMGDEVFPPLEHAGDVTAHAAFVKPEGSAPAVKLLHHAVICIAKERLWIQNPYFIPEP
                     EAIEAFGDAVARGVDVRVLMPSTSGSDNPMVQHAGHRNFEKLLRCGVRLFEYPHTLLH
                     QKIMTVDGTWCTVGSANFDDRSFETNEEITLGFRDAATTERLDRIFEKYAARANEIKL
                     EAWRRRGWKHRLIDNFYYVFNEVL"
     gene            3311..4111
                     /locus_tag="VD737_10295"
     CDS             3311..4111
                     /locus_tag="VD737_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HXW10998.1"
                     /translation="MAGEHGFEPPAIRLLTLNAHQGFAPFRRRKLLPRLRDALRASGA
                     DLVFLQEIGGDHGEDIPQQQYELLADQVWPQHAYGRNVVATMGHQGNALLSKFRIVHW
                     QNVDVSVEGVEPRGLLHCVLDVQPVGQPLHAVCVHLGLGEAQRLWQVDRLLAFVAEAI
                     PRDAPLVIAGDFNDWRERAHTRMLADPSLAEIHTSASQRPARTFPAWWPWLRLDRIYV
                     RNLHHRPLEMPRRPWSGLSDHAPLAAEICLESASIAVVASSTGAPAVR"
     gene            complement(4086..>4956)
                     /locus_tag="VD737_10300"
     CDS             complement(4086..>4956)
                     /locus_tag="VD737_10300"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HXW10999.1"
                     /translation="LRLRGTNIRALAENGVEIFFTQVFRHNFFHADMHPGNIFVQTDD
                     PERPVYAAVDFGIVGSLDASDQHYLAENFIAFFDGDYNRVARLHVDSGWVPPDTRVDE
                     LEAAVRTVCEPIFNKPLSEISFATVLMRLFEIARRFDMRIQPQLLLLQKTMLQIEGLG
                     RQLYPQLDLWKTAQPILQEWAADRYSGRSFAEQFRRQLPDINEAVRSLPQVLQQFVQK
                     ASEGTLRIRMDQTPYEELRRELRLSARRRDQTVVGAAVLLAGIVWLALRVDWMPGVAL
                     TAAGLLTVLLARR"
BASE COUNT          784 a         1689 c         1687 g          796 t
ORIGIN      
        1 gtattcgggt ggccagacga tccaggtctg tcccgcggcg gcggcgccca gcagcacgat
       61 ggctgcttcg gcaggatcgt gtcccgagtg gtccatcagc tggtgggcca ggcggttttc
      121 ctcgggcggg gcccgcatct cgtcgagcag gtgcgtgcgg aagaatcccg gcatcgccac
      181 ggacacctgc aggcccgtcc cggcgagttc ggtcacgagc gtctcgctca gggcgatcac
      241 ccccgccttg gtcacgttgt aggcggccat ctgcggcgct gccgcaaatc ccgccgaact
      301 cgcgacgttc aacacgaggc cctgtccctg gcgcttgagc accggcaacg cggcgcgaca
      361 gccccagacg acgccgagca ggttgatgtc gacgatccag cgccagtcct cgaccgcggt
      421 cagttcgacc gccccggcca cggccacccc tgcattgttg acgagcacgt cgagcccgcc
      481 gtgccgggcc acgaagtcgc ccatcgcgcc tgcgacgacc gacggcgagg ccacgtcgcc
      541 cggaaacccg gtcacgtcgc gtgcgcccga tcgccgaagg tcgctgaccg cctgctcgag
      601 tcgttcggtc gacaggtcca gcagcccgag cgaccagccg tcgcgggcca ggcattccgc
      661 catggcgaac ccgagtccgc tcgcggcgcc ggtgacgaag gcacgctttg ccggaaagcg
      721 acgggacagt aaggtagcgg gggaggtggc ggtcatgacg tcatgctaac gggccgtcac
      781 gcgtggcgaa acggaccggg ggcggcggct cgaccgacgg gatgcatcgt gcgcaaggtc
      841 tacacgggcg aatcggtgat cgaggtcgcg cacctgcgca acgtgctcga aggtgccggc
      901 atccgctgtt tcgtgcgcaa cgaggggctc gcgggcgtga tcggcgagat cccgtacgtc
      961 gagtgctggc ccgagctctg ggtggtccgc aatggcgacg cgctgcgcgc ccgggggctc
     1021 atcgacgaag ctcgccagtc ggcctccgcg cccggtccgg actggcgctg cgagcgttgc
     1081 ggcgaactcg tcgagggcca gttcgacagc tgctggcgct gcgccgagcc cagggaatga
     1141 caccgggaaa cacggacgaa ccatcatcat gaccgccagg accccgaccg gaccgacgac
     1201 gcacccggcc accgatccga ccacgatcac gatcgcgcgg cggttcaacg gcccgcccga
     1261 ttcggccaac ggcggctacg tctgtggcgc cttcgcggtt gccaccggcg aaggcctgcg
     1321 ggtgcgcctg ctcgcgccgc caccgctcga acgcccgctg gccgtccggg tgggcgacgg
     1381 ggacggctcg ttctggctgc gcgacggcga ccagcccgtc gccaaggccg tgccctcgca
     1441 gctcgaactc gaagtgcccg ccgcgccgcc gtacgtgcag gcggtgtggg cgtcgcagca
     1501 ctacgcgggc ttccgccagc acacgttccc aagctgcttc gtctgtgggc cgcaccgtcg
     1561 ccgcggcgac ggactgcgca tcttcccggg ggtgctcgat tccgggatcg tcgcggcgcc
     1621 gtggctgccc gcggacgacc tcgacggcgg cgacggcaag gtcgccgtcg agttccactg
     1681 ggccgcgctc gactgtcccg ggtacttcgc gctggtcggc gaccggccgc gcaagatgct
     1741 gctgggcgag atgcaggccc acgtcgaccg gcgggtgcac gtgggcgaat cctgcaccgt
     1801 gatcggctgg tcgatcggcg tcgacggccg caagcattac tccggcacgg cgatcttcga
     1861 cgaggacggc gagctgtgcg cgcgggcgag gacgacctgg gtcgaagtgg tcgagagagg
     1921 cgactgacgt cgagacggcg ccggccacgg ctcacagcgt gttcacagca cctcgttgaa
     1981 cacgtagtag aaattgtcga tcaggcgatg cttccagccg cgccgacgcc acgcctcgag
     2041 cttgatctcg ttggcccgcg ctgcgtactt ctcgaagatc cggtcgagcc gctcggtggt
     2101 ggccgcatcc ctgaagccga gcgtgatctc ctcgttggtc tcgaacgacc tgtcgtcgaa
     2161 gttggccgag ccaacggtgc accacgtgcc gtcgacggtc atgatcttct ggtgcagcag
     2221 cgtgtgtggg tactcgaaca gccgcacgcc acatcgcagc agcttctcga agttgcggtg
     2281 tcccgcgtgc tggaccatcg ggttgtccga tccgctcgtc gacggcatca gcacgcgcac
     2341 gtcgacgccg cgcgccactg cgtccccgaa agcctcgatc gcctcgggct ccgggatgaa
     2401 atagggattc tggatccaga gtcgctcttt cgcgatgcag atgaccgcgt gatgcaacag
     2461 cttgacggcc ggcgccgaac cctcgggctt cacgaatgcg gcgtgggcgg tgacgtcccc
     2521 ggcgtgctcg agcggcggga agacttcgtc gcccatgaac agctcaccgg tctgtccgac
     2581 ccagttctcg ctgaaggccg actgcacgct gtgcacgatg gggccgcgca gcctgacgct
     2641 gacgtcggcg aagtgcttgt cgtcctccgc gtcgccgagc caggtgtcga cgatgcagtg
     2701 gccgccgagg aacgcagtcc ggccgtccag gacgacgatc ttccggtgat cgcgctcgtt
     2761 gaagacgccg atgtggcgca agtcgaaagg gtggaacttc gtgagcttgc agccggcggc
     2821 ctcgagctgt tgctcgaccg ccttgcccat cttcttgctg ccttgcgcgt cgaccagcac
     2881 acgcacggac acgccggccc gggcgcgctc gcagaaggca tcggcgaggc gcttgccgag
     2941 cacgccttcc tcccagagaa acgtctcgaa gtgcaccgag tggcgcgccg cggcgatctc
     3001 ctccagcagc acgtcgaagt acgcgccgtt ctcgaggact tcgacgctgt tgccggccac
     3061 gcaagtgccg agcgaaagcc cggcaagcga tggcatcagc tggtcgatcg gtgcgtcgca
     3121 ctcgacctgc agcttcgggt cccggtgccg cttcatcgac cagatcacca ggaccagcag
     3181 gagaatgacg ccgaggacga tcagcgtgac cattgcatcc gcctccattg cgcaacgcgg
     3241 ctggatcatc gcatttcccg ccggcttcgg ttgagttgcg cgcatcggca gtgggatagt
     3301 caccctgcgc atggctggtg aacacggttt cgaacccccc gcaatacggc tgctgacgct
     3361 gaacgctcac cagggcttcg ccccgttccg ccgtcgcaag ctgcttcctc gcctccgcga
     3421 cgccctgcgc gcgtccggtg ccgacctggt cttcctgcag gaaatcggcg gcgaccacgg
     3481 cgaggacatt ccgcaacagc agtacgaact gctggccgac caggtgtggc cacagcacgc
     3541 ctatggccgc aatgtcgtcg cgaccatggg tcaccagggc aacgcgctgc tgtcgaagtt
     3601 tcgaatcgtt cactggcaga acgtcgatgt gtccgtcgag ggcgtcgagc cgcgcgggtt
     3661 gctccattgc gtgctggatg tgcagccggt ggggcagccg ctgcacgcgg tgtgcgtaca
     3721 cctgggcctg ggcgaggcgc aacgcctgtg gcaggtcgat cggttgctgg cgttcgtggc
     3781 cgaggcgatc ccgcgcgacg cgccgctggt gatcgccggc gacttcaatg actggcgtga
     3841 gcgagcgcac acgcggatgc tcgccgaccc gtcgcttgcc gagatccaca ccagcgcgag
     3901 ccagcgtccc gcccgcacct tcccggcatg gtggccgtgg ctgcggctgg atcggatcta
     3961 cgtgcgcaac ctgcaccacc ggccgctcga gatgccgcgc cgtccctggt ccggcctgtc
     4021 ggaccatgca ccgcttgcgg ctgaaatttg cctggaatca gcttcgatcg cggtcgtggc
     4081 atcgtctacc ggcgcgccag cagtacggtg agcagccccg ccgccgtcag cgccacgccg
     4141 ggcatccagt cgacgcgcag cgcgagccag acgatgccgg cgagcagcac ggcggcgccc
     4201 acgacggtct ggtcgcgacg ccgcgcgctc aggcggagct cgcggcgcag ttcctcgtac
     4261 ggcgtctggt ccatgcggat ccgcagggtg ccttcgctcg ccttctgcac gaactgctgc
     4321 agcacctgcg gcagcgagcg caccgcctcg ttgatgtcgg gcagctggcg gcggaactgc
     4381 tcggcgaaac tgcggccact gtagcgatcg gcggcccatt cctgcaggat cggctgagcc
     4441 gtcttccaga ggtcgagctg gggatagagt tgccggccca gcccttcgat ctgcagcatg
     4501 gtcttctgca gcagcagcag ttgcggctgg atgcgcatgt cgaaccgtcg cgcgatctcg
     4561 aacaggcgca tcagcacggt ggcgaacgag atctcggaga gcggcttgtt gaaaatcggc
     4621 tcgcacaccg tgcgcacggc cgcctcgagc tcgtcgactc gcgtatccgg cggcacccag
     4681 cccgaatcaa cgtgcaggcg cgcgacacgg ttgtagtcgc cgtcgaagaa cgcaatgaag
     4741 ttctcggcga ggtagtgctg gtcgctcgcg tcgagcgaac ccacgatgcc gaaatcgaca
     4801 gcggcataca ccggacgctc gggatcgtcg gtctgcacga agatgttgcc gggatgcatg
     4861 tcggcgtgga aaaagttgtg ccggaagacc tgcgtgaaga agatctccac gccattctcg
     4921 gccagggccc ggatgttggt gccgcgcagg cgcagt
//
LOCUS       DASPFR010000388         2578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_847966, whole genome shotgun sequence.
ACCESSION   DASPFR010000388 DASPFR010000000
VERSION     DASPFR010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847966"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1561
                     /locus_tag="VD737_10305"
     CDS             <1..1561
                     /locus_tag="VD737_10305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HXW11000.1"
                     /translation="ALRAQGWHGRRLPSQWALEFAAALRQVGWPGAAPDSAEHQAVQR
                     WQALLAEFGAGDDVAGPMRLRDALAHLRDQLLHTAFEPQEIAAPVLVIDPATAVGMRF
                     DAIWICALDATRWPAPANPDPFVPREWQARQGVPGATAELAESHARRTLAGLTHGAAV
                     AICSVPRFEDDAPLLPSALVAGLPLRSDLPLWNGAGTTRSQFARRPPLERLVDSALPA
                     FAAHQVAKGGTRLLELQAACPFRAAIELRLGGGALEDAVVGIAPTERGRLVHAVLQTF
                     WGEVREQSALLAMSPRDRERCVHEHADRALAPLRDSADEVRRRLLDLEQGWLEARVLE
                     LLDQDAQREPFSVVHVEEPRVVDVGGVQIKVQLDRVDRLADGSHAVIDYKTSAAAKPS
                     AWMGERPQLPQLPLYVRAVGAPTVSAVAFGVVRKGGTSYSGFVSADGVFTQLEPFDPA
                     RLPFRGYPDWQSLMAEWERRLDALALEHAAGDARLAPDPARACRYCHLPGLCRSAEAR
                     VDAEEGTDAAA"
     gene            1548..>2578
                     /locus_tag="VD737_10310"
     CDS             1548..>2578
                     /locus_tag="VD737_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXW11001.1"
                     /translation="MQPRELLEKDAEARRTALDVSRSFIVQAPAGSGKTELLTQRFLA
                     LLAVVDRPEALLAITFTRKAAAEMRNRILEALRHCDDVRSNLREDTRGLARRALQADR
                     KQGWRLLENPSRLRVLTIDALNQSLARRLPVLSGLGAGLRVDEDGRELYADAAERLLG
                     HLPSGEPRAAAAVTRVLDHLDNNVGLFVRLIGAMLARREAWLPVLPDDVDAYDDEGSV
                     RDALEDGRRAIVATHLRQLVDAIPPGLLDELWSLAHGAAQVLETLGWESPIRACGPEV
                     PGAQAADLDRWRGLAEFLFTRKDELRASFNRTVGVLPQQRSRPDLAIVKARVAAAARR
                     LAQHEDACA"
BASE COUNT          335 a          926 c          950 g          367 t
ORIGIN      
        1 cgcgctgcgt gcacagggct ggcacgggcg gcgtctgccg agccagtggg cgctcgaatt
       61 cgccgcggcc ctgcgccagg tcggctggcc cggcgcggcg cccgacagcg ccgagcacca
      121 ggcggtgcag cgctggcagg cgctgctggc ggagttcggc gcgggtgacg acgtcgccgg
      181 cccgatgcgc ctgcgcgacg ccctcgcaca cctgcgtgac cagctgctgc acaccgcctt
      241 cgaaccgcag gagatcgccg cgcccgtcct ggtgatcgat cccgccaccg ccgtcggcat
      301 gcgattcgac gcgatctgga tctgcgccct ggacgcgacg cgctggcctg cgccggcgaa
      361 ccccgacccg ttcgtgcctc gcgaatggca ggctcgccag ggcgtgccgg gcgcgaccgc
      421 ggaactcgcc gagagccacg cccgccgcac gctggcgggc ctcacgcacg gcgcggcggt
      481 cgcgatctgc agcgtgcccc gcttcgagga cgatgcaccg ctgctgccga gcgcgctcgt
      541 cgcgggcctg cccttgcgaa gcgacttgcc actgtggaat ggcgcaggca ccacgcgatc
      601 acagttcgcg cgtcgcccac ccctcgaacg gctcgtcgac tcggcactgc cggcattcgc
      661 cgcgcaccag gtagccaagg gcgggacgcg tctgctggag ctgcaggcgg cgtgtccctt
      721 ccgcgccgcg atcgagttgc gcctgggcgg cggcgcgctc gaggacgccg tcgtgggcat
      781 cgcgccgacc gagcgcggcc ggctcgtcca cgcggtgctg cagacgttct ggggcgaggt
      841 gcgcgagcag tccgccctgc tggccatgtc cccacgggat cgcgagcggt gcgtgcacga
      901 acacgccgac cgggcgctcg caccgctgcg cgactccgcc gacgaggtgc gacgccggct
      961 gctcgacctc gagcagggct ggctcgaagc gcgcgtgctc gaactgctcg accaggacgc
     1021 gcagcgtgag ccgttctcgg tcgtgcacgt cgaggagccg cgtgtggtgg acgtgggtgg
     1081 cgtgcagatc aaggtgcagc tcgaccgcgt cgaccggctg gccgacggca gccacgcagt
     1141 gatcgattac aagacgagcg ccgccgcgaa accgtccgcg tggatgggcg agcgccctca
     1201 gcttccacag ctgccgctgt acgtgcgtgc ggtcggtgcg cccaccgtga gtgccgtcgc
     1261 gtttggcgtc gttcgcaagg gtggcacgag ctactcgggg ttcgtgagcg ccgacggcgt
     1321 gttcacgcag ctcgagccgt tcgatccggc acgcttgccg ttccgcggat acccggactg
     1381 gcagtcgttg atggcggagt gggagcgtcg gctcgacgcg ctcgccctcg agcacgccgc
     1441 aggcgatgcg cggctcgcgc ccgacccggc ccgcgcctgc cggtactgcc atctgccggg
     1501 gctgtgccgg tccgcggagg cgcgggtcga cgccgaggag ggcaccgatg cagccgcgtg
     1561 aactgctcga gaaggacgcg gaggcgcgtc gcacggcgct cgacgtttca cgcagcttca
     1621 tcgtccaggc gccggcgggg tccggcaaga ccgaactgct gacgcagcgc ttcctcgcgt
     1681 tgcttgccgt cgtggaccgt cccgaggcgt tgctcgcgat cacgttcacg cgcaaggcag
     1741 ccgccgagat gcgtaaccgc atcctcgaag cgctgcgcca ttgcgacgac gtgcgatcga
     1801 acctgcgcga ggacacccgc ggactcgcgc ggcgtgcgct gcaggccgac cggaagcagg
     1861 gctggcgact gctcgagaat ccgagccgcc tgcgcgtgct gacgatcgac gcgttgaacc
     1921 agtcgctcgc acgccggctg cccgtgttgt cggggctcgg cgcgggcctg cgcgtcgacg
     1981 aggacggtcg cgaactctac gccgacgcgg ccgagcgcct gctcggacac ctgccgtcgg
     2041 gtgagcctcg cgcggccgcg gccgtcacgc gcgtgctgga ccacctcgac aacaacgtcg
     2101 ggcttttcgt gaggctgatc ggggcgatgc tcgcacggcg cgaggcctgg ctgccggtgc
     2161 tgcccgacga cgtcgacgcg tacgacgacg aaggttcggt ccgcgatgcc ctcgaggacg
     2221 gtcgtcgcgc gatcgtggcg acgcacctgc gccagctggt cgacgcgatc ccgcccggac
     2281 tgctggacga gttgtggtcg cttgcgcacg gcgccgcgca ggtcctcgag acgctcggct
     2341 gggaatcgcc gatccgcgcc tgcggcccgg aggtccccgg tgcgcaggcg gccgacctcg
     2401 atcgctggcg cgggctcgcc gaattcctgt tcacgcgcaa ggacgaactg cgcgcgagtt
     2461 tcaaccgtac ggtgggcgtg ctgccgcagc agcggtcgcg tccggacctc gccatcgtca
     2521 aggcgcgcgt cgcggcggcc gctcggcggc tcgcgcagca cgaggacgcg tgcgcgtt
//
LOCUS       DASPFR010000389         4718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1579824, whole genome shotgun sequence.
ACCESSION   DASPFR010000389 DASPFR010000000
VERSION     DASPFR010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579824"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VD737_10315"
     CDS             <1..603
                     /locus_tag="VD737_10315"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HXW11002.1"
                     /translation="DGESGISRPGILLRDAIQRANKIVYQTARTQANCDGMGTTVVAG
                     LFFDDRLTVAHVGDSRLYRLREGKLEQVTKDHSLLQELVARGFYTPEEAQRAAAKNYV
                     TRALGVEPTVDVEITETPVTKDDVYLLCSDGLSDMVDDADIQLTINTFGANLETVARQ
                     LVQLSNDNGGRDNVSVVLVRVLDAFPAQRGMIDRILGIFG"
     gene            620..1360
                     /locus_tag="VD737_10320"
     CDS             620..1360
                     /locus_tag="VD737_10320"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXW11003.1"
                     /translation="MARLVLHLDGQTLAEYNMSKERYTVGRLPDNDIRIDNPAVSGHH
                     ALVINILNDSFLEDLNSTNGTYVNGKIIKKHALQHGDVITVGHHALRFVDSETEDEQD
                     EFEKTMVIGGREAAQLIAGAQQKAAAAAAPAANGHAAVSPAGGASGAPAFANGVLPRA
                     KLQVLSGQFAGRELDLVKTLTTLGRPGVQVAAITRRADGFYIVHVESGRADDFPMVNG
                     APIGQQARRLHDNDVVQLAGVKMGFFEN"
     gene            complement(1460..2551)
                     /gene="alr"
                     /locus_tag="VD737_10325"
     CDS             complement(1460..2551)
                     /gene="alr"
                     /locus_tag="VD737_10325"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HXW11004.1"
                     /translation="MSSTNLIRAVVDADALRHNLREVRKVAPESRIMAVIKANAYGHG
                     LVPAAQALAGSDGFAVARLDEGLALRAAGLGNRILLLEGVVTLEQLQIAARERFELMV
                     HSFEQLELLDALDAAGPALPIWVKVDSGMNRLGFRLEEFPDAYARLRRNPRVQPAPTL
                     VTHLASADDRNDSGTQKQLDAFTRATAGLPGARSIANSAGVLAWPASRADWVRPGLML
                     YGVSPFEQGSGAALGLRPAMTLCTEVIAVKAVRAGEGVGYGGTWTASRDTRMAVIAAG
                     YGDGYPRSMSSGAPVVVNGRRARLVGRVSMDMITVDVTDLPPVTVGDPVVLWGAEVPV
                     EELAAHAGTIAWDLLCGVSQRVELEVRSG"
     gene            complement(2577..4031)
                     /gene="dnaB"
                     /locus_tag="VD737_10330"
     CDS             complement(2577..4031)
                     /gene="dnaB"
                     /locus_tag="VD737_10330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007902695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HXW11005.1"
                     /translation="MEQTDSSDGGRGSARSRRGPRLVPGDDAAALRVPPHSLEAEQSV
                     LGGLLLDNSTWDSIADRLRPDDFYRRDHQLIFEAILDLSQRGEPSDAVTLAERLASKG
                     LGDDTGGLAYLAGLARDTPTAANIRAYADIVRERSLLRQLLRISGEIAGSCFEAEGRP
                     ASELVDEAERRIFEIAEKGRRQGSGFVAVREVLGAAIDRLDLLHASQGQLTGLSTGYS
                     DLDRMTAGLQPGDLIVVAGRPSMGKTTFALNIAENAAIAFNKPVAVFSMEMSREQLAF
                     RMISSLGRVDQGHMRTGMFGDEDWARINGAIAQMKNAPMYIDDSGALTPTEVRARARR
                     LARDTEKLGGLGLIVVDYLQLMQVPGNKENRATEISEISRSLKALAKELKVPLIALSQ
                     LNRGVEQRTDKKPVMSDLRESGAIEQDADLIMMIYREEVYEPDTPRKGIADIIVAKQR
                     NGPTGEVHLTFLGKYTRFENLAHGDYGYDGGPQY"
     gene            complement(4071..4520)
                     /gene="rplI"
                     /locus_tag="VD737_10335"
     CDS             complement(4071..4520)
                     /gene="rplI"
                     /locus_tag="VD737_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HXW11006.1"
                     /translation="MEVILLQKVANLGNIGDKVKVKPGYGRNFLLPSGRAALATAANL
                     KKFEERRTELEKAAADSLTRARARAKQLEDFKLELHAKAGTEGKLFGSIGTADIAEAL
                     TKAGIEIERSEVRLSGGPIRLVGEHHVKLHLHSDVEIDLPVHVTAED"
BASE COUNT          756 a         1602 c         1607 g          753 t
ORIGIN      
        1 gacggcgaga gcgggatcag ccgaccggga atcctgttgc gtgacgcgat ccagcgcgcc
       61 aacaagatcg tgtaccagac cgcccgcacg caggccaact gcgacggcat gggcacgacg
      121 gtcgtggccg ggctgttctt cgacgatcgg ctcacggtgg cgcacgtcgg cgattcgcgg
      181 ctctaccgac tgcgggaagg caagctcgag caggtcacga aggaccactc gctgctgcag
      241 gaactcgtgg cgcggggctt ctacacgccc gaggaggccc agcgggcggc cgccaagaac
      301 tacgtcaccc gcgcgctcgg cgtggaaccc accgtcgacg tcgaaattac cgagacgccc
      361 gtcacaaaag acgacgttta cctgctgtgc tcggacgggt tgtccgacat ggtggacgac
      421 gcggacattc agttaacgat caacaccttt ggtgctaacc ttgagacagt cgcgcggcag
      481 ctggtgcagc tgagcaacga caacggggga cgcgacaacg tctccgtggt gctcgtccgg
      541 gtgctcgacg cattcccggc gcagcgcgga atgatagacc ggatactcgg aatattcggc
      601 tagggagcgc aggacgaaca tggccaggct ggtattgcat ctcgacgggc agacgctcgc
      661 cgagtacaac atgagcaagg agcgctacac ggtagggcgc ctgccggaca acgacatccg
      721 catcgacaac ccggccgtca gcgggcacca cgcgctggtc atcaacatcc tcaacgactc
      781 gttcctcgag gacttgaaca gcaccaacgg cacgtacgtc aacggcaaga tcatcaagaa
      841 acacgcgctg caacacggcg acgtgatcac ggtcggccac cacgccctgc ggttcgtcga
      901 cagtgaaacc gaggacgagc aggacgagtt cgagaagacg atggtgatcg gcgggcgcga
      961 ggccgcgcag ttgatcgccg gcgcgcagca gaaggcggcc gctgcagcgg cgccggcggc
     1021 gaacggtcac gcggcagtga gcccggcagg gggcgcgagc ggcgcaccgg catttgccaa
     1081 cggcgtgttg ccccgcgcga agctgcaggt gctgtcggga cagttcgcgg gacgcgagct
     1141 cgacctcgtc aagacgctga cgacgctcgg ccgtcccggc gtgcaggttg ccgcgatcac
     1201 ccgccgcgcc gacgggttct acatcgtcca cgtcgaaagc ggccgcgccg acgacttccc
     1261 gatggtcaac ggtgccccga tcgggcagca ggcccgccgc ctccacgaca acgacgtcgt
     1321 gcagctcgcg ggggtgaaga tggggttctt cgagaattag tggctagcgg ttagtggtta
     1381 gcggttagtt ggaaggcggc cagaattggc cgccttcttt ttgactaacc gctaaccgct
     1441 aaccgctaac cgctaaccgc taaccgctgc ggacttccag ttccacccgc tggctcaccc
     1501 cgcacagcag gtcccacgcg atcgtgccgg cgtgggcggc cagttcctcg acgggcactt
     1561 cggcccccca caacacgacc gggtcgccca cggtcacggg cggcaggtcg gtgacgtcga
     1621 cggtgatcat gtccatggac acgcggccga ccaggcgcgc acggcgccca ttgacgacga
     1681 cgggcgctcc ggacgacatg ctgcggggat aaccgtcgcc gtagcctgcc gcgatcacgg
     1741 ccatgcgcgt gtcccggctc gcggtccacg tgccgccgta cccgacccct tcgcctgcac
     1801 gcacggcctt cacggcgatg acctcggtgc agagcgtcat cgcgggtcgc agccccagcg
     1861 cggccccgct gccctgctcg aacggcgaga cgccgtacag catcaggccc gggcgcaccc
     1921 agtcggctcg cgaggcaggc caggccagca ccccggcact gttggcgatg ctgcgcgcgc
     1981 ccggcaggcc ggcggtcgcc cgcgtgaacg cgtccagctg tttctgggtg cccgagtcgt
     2041 tacggtcgtc ggcgctcgcg aggtgcgtca cgagcgtcgg tgccggctgc acgcgtggat
     2101 tgcgccgcag ccgggcgtag gcgtcgggga attcctcgag acgaaagccg agccggttca
     2161 tgccggagtc gaccttgacc cagatcggca atgccgggcc ggccgcatcg agtgcgtcga
     2221 gcagctcgag ttgctcgaag ctgtgcacca tcagctcgaa gcgttcgcgg gcggcgatct
     2281 gcagttgctc cagcgtgacg acgccctcga gcagcaggat gcgattgccg agtccggccg
     2341 cgcgcagcgc cagcccctcg tcgagacgcg ccaccgcaaa accgtcggat cccgccaggg
     2401 cctgcgcagc cggcaccagc ccgtgcccgt acgcgttcgc cttgatcacc gccatgatgc
     2461 ggctctcggg cgcgaccttg cgaacctcgc gcaggttgtg acgcagtgcg tcggcgtcga
     2521 cgacggcgcg gatgaggttg gtgctgctca agggcgacgc aatgacgcga cgacgatcag
     2581 tactgcgggc cgccgtcgta gccgtagtcg ccgtgcgcca ggttctcgaa gcgcgtgtac
     2641 ttgccgagga acgtcaggtg cacttcgccg gtggggccgt tgcgctgctt ggcgacgatg
     2701 atgtcggcga tgcccttgcg cggggtgtcg ggttcgtaca cctcctcgcg gtagatcatc
     2761 atgatcaggt ccgcgtcctg ctcgatcgca cccgattcgc gaaggtcgga catcaccggc
     2821 ttcttgtcgg tgcgctgctc gacgccgcgg ttgagctgcg acaacgcgat cagcggcacc
     2881 ttgagttcct tcgcgagcgc cttgagcgaa cgcgagatct cggagatctc cgtcgcgcgg
     2941 ttctccttgt tgccgggcac ctgcatcagc tgcaggtaat cgaccacgat caggccgagg
     3001 ccgccgagct tctcggtgtc gcgcgcgagc cgccgagcac gcgcgcgcac ttcggtcggc
     3061 gtgagcgcgc cgctgtcgtc gatgtacatc ggcgcgttct tcatctgggc gatcgcaccg
     3121 ttgatgcgcg cccagtcctc gtcgccgaac atgccggtgc gcatgtgacc ctggtcgacg
     3181 cggccgagcg acgagatcat gcggaacgcg agctgctcgc gcgacatttc catcgagaac
     3241 acggcgacgg gcttgttgaa cgcgatcgcg gcgttctcgg cgatgttgag cgcgaacgtc
     3301 gtcttgccca tcgacgggcg gcccgcgacg acgatcaggt cgccgggctg caggccggcg
     3361 gtcatcctgt cgaggtcgga gtacccggtg ctcaggcccg tgagctggcc ctgcgacgcg
     3421 tgcagcaggt cgagtcggtc gatcgcggcg ccgagcacct cgcgcacggc cacgaagccc
     3481 gagccctgcc ggcggccctt ttccgcgatc tcgaagatgc gccgctcggc ttcgtcgacc
     3541 agctcgctgg ctggccggcc ctcggcttcg aagcagctgc cggcgatctc gccgctgatg
     3601 cgcaacagct gccgcagcag cgatcgctcg cggacgatgt ccgcatacgc cctgatgttc
     3661 gcggccgtgg gcgtgtcgcg cgcgaggccg gcgaggtacg cgagaccccc ggtgtcgtcg
     3721 ccgaggccct tgctcgcgag gcgctcggcc agtgtgacgg cgtcgctcgg ctcaccgcgc
     3781 tgcgacaggt cgaggatcgc ctcgaagatc agctggtggt cgcgccggta gaaatcgtcc
     3841 ggccgcagcc ggtcggcgat cgaatcccag gtgctgttgt cgagcagcag gccgccgagc
     3901 acggactgct cggcttcgag cgaatgcggc ggcacgcgca gcgccgccgc gtcgtcgccc
     3961 ggaacgaggc gcggaccccg ccgtgagcgc gcggagcccc ggccgccgtc gctgctgtct
     4021 gtctgctcca tcgaggaccc atgctacccc ggctcggccg ccgggacaga tcagtcttcg
     4081 gcagtcacgt ggaccggcag gtcgatctcg acgtcgctgt ggaggtgcag cttgacgtga
     4141 tgctcgccga cgaggcggat cgggccgccc gacaggcgca cttcactgcg ctcgatctcg
     4201 atgccggcct tggtcagggc ctcggcgatg tcggcggtac cgatggagcc gaacagcttg
     4261 ccctcggtgc cggccttcgc gtgcagttcg agcttgaagt cctcgagctg cttcgcgcgg
     4321 gcgcgagcac gcgtcagcga gtccgccgcg gccttctcca gctccgtgcg ccgttcctcg
     4381 aacttcttga ggttggcggc ggtcgcgagc gcagccctgc ccgagggcag caggaaattg
     4441 cggccgtagc cgggcttcac cttgaccttg tcgccgatgt tgccgaggtt ggcaaccttc
     4501 tgcagcagga tgacttccat cgttgtgtcc tcgaatcgat ctctatcggt gcaatcagtt
     4561 accagtcggc agcgagccgc cagccccggc ccgcaggcga tgcctcactg ccggccggcc
     4621 gtcgtcgtcg ccatgcgttg cgagcgcagc cagttgtcga cgaatcccca gcctgcgagc
     4681 gcgatcacca tgggcgcggc cgtcacgatc aggccgag
//
LOCUS       DASPFR010000390        10648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_117236, whole genome shotgun sequence.
ACCESSION   DASPFR010000390 DASPFR010000000
VERSION     DASPFR010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10648
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117236"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="VD737_10340"
     CDS             <1..1142
                     /locus_tag="VD737_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXW11007.1"
                     /translation="DVTRVAKEAIEAPRVVRVFNAQAYEARLFDEVAEHNRRSHMKLM
                     MTKGLSNPIVQTIAATGLAGVLYLATVDAIAGRMTVGEFTSFIAALMLITAPLRRLVN
                     VAGPLQQGIAAAQSIFEVLDAPVEHAGGTHRVERVRGAIEYRDVGFQYPMSADPVLHG
                     IGFEARPGETVAIVGRSGSGKSTLVGLLPRFYDATSGQVLLDGVDVREYALDALRSQV
                     SLVSQDVVLFNDTIRSNIAFGHAADDAQIEAAARAAGVTEFSEQFPLGLDTVVGDRGA
                     LLSGGQRQRIAIARALLRNTPVLILDEATSALDTELERQIQGQLEALMADRTTLVIAH
                     RLSTVEKANRILVMEAGRVTESGTHQELIARGGQYAVLHRLQFSE"
     gene            1149..2195
                     /gene="lpxK"
                     /locus_tag="VD737_10345"
     CDS             1149..2195
                     /gene="lpxK"
                     /locus_tag="VD737_10345"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HXW11008.1"
                     /translation="MSLDAALQRLWYGPAWHALPLWPLALLFRGVVALRAAAYRLGIL
                     RSHEVGRPVIVVGNLTVGGTGKTPVAAWLARALSQRGHRVGVVLRGYGGGHHGPPHIV
                     ELSDNPANVGDEALLHARRDVHVVVIGADRVAAARHAVEHGADVVVCDDGLQHRRLAR
                     DYEIAVVDGARGLGNGLPLPAGPLREPARRLESVNAVVVTHRAAAATPPPFEVRTPLL
                     VDAHLSLDSAVNLVSGERRPLQAFGDAQSLHALAAIGHPDAFFRALRDAGLRPVEHAL
                     PDHAALDPQALPFRPDATVLMTEKDAVKCREFGQPGWWWVDLEVTVDRTDAETLLASI
                     HERTGLAGAGAVLG"
     gene            2188..2376
                     /locus_tag="VD737_10350"
     CDS             2188..2376
                     /locus_tag="VD737_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008375064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HXW11009.1"
                     /translation="MDSKLLDILACPLCKGPLQWHRDQECLVCRADRLAFPVRDDIPI
                     MLEDEARPLKSDDPLLAD"
     gene            2394..3179
                     /gene="kdsB"
                     /locus_tag="VD737_10355"
     CDS             2394..3179
                     /gene="kdsB"
                     /locus_tag="VD737_10355"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004645470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HXW11010.1"
                     /translation="MGFHVVIPARHASTRLPGKPLLDIGGRPMIQRVVEQASASGAEQ
                     VLVATDDERIVAAVHDPRRHSQRIALMTDPDLPSGTDRVAAVARSLGWNAATIVVNVQ
                     GDEPFVPPVLIDQVAALLEHDVRADVATLAAPMGTLAEFMDPNVVKVVMAADGSALYF
                     SRAPIPWARDGAPGGLTSQTRFDGALRHVGLYAYRVGALERLTRLSPSPLEQVEKLEQ
                     LRALQAGLRIVVAHCAVPAAPGVDTEHDLERARARVHRGAEPA"
     gene            3176..3490
                     /locus_tag="VD737_10360"
     CDS             3176..3490
                     /locus_tag="VD737_10360"
                     /inference="COORDINATES: protein motif:HMM:NF019214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HXW11011.1"
                     /translation="MNEPFHVLWFVTLTPWKLVGYLGVTLFAGRWFVQLYYSRKLGRP
                     VVPTVFWLMSISGSLLLLSYFTFGKNDSVGVLSNLFPAGVAAYNLYLDLANRRSRSAA
                     DA"
     gene            complement(3551..4276)
                     /gene="rluF"
                     /locus_tag="VD737_10365"
     CDS             complement(3551..4276)
                     /gene="rluF"
                     /locus_tag="VD737_10365"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019301304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HXW11012.1"
                     /translation="MRLNKFLSESGACSRREADAWIEAGRVQVNGATAVLGTQVADGD
                     EVLLDGRPVAARPQRVYLALNKPIGIECTTDRGVPDNIVDFVAHRERIFPIGRLDKES
                     EGLILLTNDGDIVNRVLRAEHEHEKEYIVSVDRPLTADFLAGMATGVPILGTVTNPCK
                     VRQVGRNTFRIVLTQGLNRQIRRMCEHFDYTVRRLQRVRIMHIHLGDLPIGKWRPLTD
                     AEVRPLLPLERSARRATLTLKKG"
     gene            complement(4300..4662)
                     /locus_tag="VD737_10370"
     CDS             complement(4300..4662)
                     /locus_tag="VD737_10370"
                     /inference="COORDINATES: protein motif:HMM:NF005297.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor Sui1"
                     /protein_id="HXW11013.1"
                     /translation="MSSQRIVYSTGVGRRCPECGRTVEDCRCRRSKPAQPVTPAGDGI
                     VRVGRETQGRRGKGVTVIRGLPLAGDALAELATRLKKRCGSGGTVDAGVIEIQGDHRD
                     LLVEELGKLGYRVRRAGG"
     gene            4853..5275
                     /locus_tag="VD737_10375"
     CDS             4853..5275
                     /locus_tag="VD737_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXW11014.1"
                     /translation="MATTQIFVNLPVQDLDRSRQFFGALGYRFDPQFTNQDAACMIVE
                     EGIYVMLLTEGFFRTFCPGKEVCDTGSSAEVLVCLTCPTREHVDDLVRKAVAAGGSTP
                     RPPQDQGFMYGHAYEDLDGHIWELIAMTPAEEKAADTR"
     gene            5272..6255
                     /locus_tag="VD737_10380"
     CDS             5272..6255
                     /locus_tag="VD737_10380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXW11015.1"
                     /translation="MSTAAETPAVCETPRLRLRQVTEDDAPFILELLNDPGWLRFIGD
                     RGVHTLEDARRYIEQGPRRMYADHGFGLLLVERKHDGAALGLCGLIRRETLPDVDLGF
                     ALAERFRGQGYACEAAAATLRHARETLKLTRVVAIAMPENVASTRLLERLGLRFERTI
                     TFGPAAETLNYFGTTAAFAFDAENVHAVGDRLWTSGQLSRRDIERLPALGVDAVVNLA
                     PPTSSNALPGEAEAVTGLGLTYVQIPVPWDRPDLRQLQQFFGVMDTLAGRHVWIHCAR
                     NMRVSAFVYLYRRLRRGEPDAAARHPMDDVWAPAGTWREFIDRALEAAAAG"
     gene            6532..7002
                     /locus_tag="VD737_10385"
     CDS             6532..7002
                     /locus_tag="VD737_10385"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HXW11016.1"
                     /translation="MRVLFVCMGNICRSPTAEGVFREFARRHAPDLELEIDSAGTHDY
                     HVGEPPDPRALKAASTRGLDISGLRARQVEAADFERFDLILAMDRLNYETLRERSPPA
                     FRSRIRLLLEFADDASREDVPDPYYGGPKGFEEVLDLLEDAAAGLLAEVRRKAS"
     gene            7029..8846
                     /locus_tag="VD737_10390"
     CDS             7029..8846
                     /locus_tag="VD737_10390"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HXW11017.1"
                     /translation="MPSQASFARSLCAWGAAAALTVVSLQLTVAYAATTGPAFARALP
                     ELPFDRILRYDEMTRLLEQWAAARPALVRLESIGRTPEGRELWFLTISNSATGPAADK
                     PALLVDGHMHAIEGPGNVAALNFVWTLLRDYGKDQRVTRLVDTRTVYVLPRLTPDGVE
                     AVLRDDRIIRSAIRPAPAEAAPDGLEMRDLDGDGRIVFMRYRDPLGPWKAHPSDARLL
                     IPRASGEREGEFWRVVPEGEIEGYDGTNITVQPALAGIDLGVFFPDPRDPVPPGSASG
                     PDPVRAPEAAAYTAAILARPNIVTHVSCHSFGGAILLPPVNADEDMPQADRLAYDALA
                     GRGAALTGYDAMTYLSLRGGRGLDAHIPTEIGWLYNRLGVYAFITEFWNPLRAAGIEL
                     EGPMSLWLGGNHPVEDDLKLLKWNDTELQGRGFVPWHAFRHRQLGDVEIGGWDKVNFW
                     YNAPYTRLREEVMPHAQWLTELALASPRLEVRSVSATPVGDRRWRVRAVLQNTGWLPT
                     SGSQRAVDVEAVGDVEAALELPPGARLVEGERVREAGQLAGLSEQRSLSTWWGYAPGT
                     PDIATVDWLVEAADGATLGVEARHRRAGVARAEVVLRRP"
     gene            9012..>10648
                     /locus_tag="VD737_10395"
     CDS             9012..>10648
                     /locus_tag="VD737_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXW11018.1"
                     /translation="MEQYFSIPREEAALVLDEFEPSVCRGGDWLFRQGDAADCLYLLA
                     RGRMQVWLGQASGDREERLVAEVNPGETIGEIGMLTGGARSASVRAVRNSLLLRMTSA
                     AFDRLARQRPDLTRHIAGSIAGRLRDRTAGVSHIRRTLKTIALLPLGPMSPVEALSQR
                     LRDALATYGSTCVLTPQTMREAGAPPLPTARHEGLSPAMVDWLAVKEDEHRFVLYVGD
                     PSEVFSDIALHHADLILLVAQASDDAAVRPWERAAFESAAAPVARRALVLCHPGADER
                     LENTAAWLEERKLDFHLHVRDPVRNDLDRLARVIAGRAIGLVFGGGAARGFAHLGVYR
                     ALHEAGITVDWLGGASIGAVMAASLATGPEPAVAIERARVAFVAGKPFGDVTLPIISF
                     LRGRRMERLIDANLPGRIEDLPIPFFCVSSNLGNGSVQVHDRGSLPRALRASVSLPGV
                     FPPAVVNGQLAVDGGILDNLPVDLMRARPVGRVIAVDLSSRKDYTVDYDSVPSPWQVL
                     AGRTLPLSRRYRVPSPVSLMLMAMSIGAIGAARAAGAR"
BASE COUNT         1522 a         3707 c         3768 g         1651 t
ORIGIN      
        1 gcgacgtgac acgcgtggcg aaggaggcga tcgaggcgcc gcgggtcgtg cgcgtgttca
       61 acgcgcaggc ctacgaggca cgcttgttcg acgaagtggc cgagcacaac cggcgctcgc
      121 acatgaagct catgatgacc aagggcctga gcaatccgat cgtgcagacg atcgcggcga
      181 cgggcctcgc aggcgtgctg tacctcgcca ccgtcgacgc catcgccggc cgcatgacgg
      241 tcggcgaatt cacgtcgttc atcgccgcgc tgatgctgat cacggcgccc ctgcggcggc
      301 tggtgaacgt ggcgggtccg ttgcagcagg gcatcgccgc ggcgcagagc atcttcgagg
      361 tgctcgacgc gccggtcgaa cacgccggcg gcacgcatcg cgtggagcgg gtccgcggcg
      421 cgatcgagta ccgcgacgtg gggttccagt acccgatgtc ggccgatccg gtcctgcacg
      481 ggatcggctt cgaggcgcgg cccggggaga cggtcgcgat cgtcggccgc tcgggcagcg
      541 gcaagtcgac gctggtcggg ctgctgccga ggttctacga cgccaccagc ggacaggtgc
      601 tgctcgacgg cgtcgacgtg cgcgagtacg cgctcgacgc gctgcggtcg caggtcagcc
      661 tcgtcagcca ggacgtcgtg ttgttcaacg acaccatccg cagcaacatc gcgttcggcc
      721 acgcggcgga cgacgcgcag atcgaggccg cggcgcgcgc ggccggggtc accgagttct
      781 cggagcagtt tccgctcggg ctcgacaccg tcgtgggcga ccgcggcgcg ctgctgtccg
      841 gcgggcagcg ccagaggatc gcgatcgcgc gcgcgttgct gcgcaacacg cccgtgctga
      901 tcctcgacga ggcgacctcc gcgctcgaca ccgagctcga gcgccagatc caggggcagc
      961 tcgaggcgct catggcggac cgtacgacgc tggtgatcgc gcaccgcctt tcgaccgtgg
     1021 agaaggcgaa tcggatcctc gtcatggagg cgggcagggt gaccgagtcc ggcacgcacc
     1081 aggagttgat cgcgcgcggc ggccagtacg ccgtgctgca ccggctgcag ttcagcgagt
     1141 aaggcggcgt gtcgctcgac gccgcgctgc agcgactctg gtatgggccc gcgtggcacg
     1201 cgctgccgct gtggccactg gccctgctgt tcaggggcgt cgtcgcgcta cgcgccgcag
     1261 cctacagact cggcatcctg cgctcgcacg aggtcggcag gcccgtcatc gtcgtaggca
     1321 acctgacggt gggtggcacc ggcaagacgc cggttgccgc gtggctcgcg cgtgcgctga
     1381 gccagcgcgg gcaccgtgtc ggcgtggtgc tgcgcggcta cggtggtggt catcatggtc
     1441 cgccgcacat cgtcgaattg tccgacaatc cggcgaatgt gggggacgag gcactgctcc
     1501 acgcgcggcg cgacgtgcac gtcgtcgtga tcggcgccga ccgggtcgcg gccgcgcgac
     1561 atgcggtcga gcacggcgcg gacgtcgtcg tgtgcgacga cgggctgcag caccggcgcc
     1621 tggcccgcga ctacgagatc gccgtcgtcg acggcgcacg cggcctcggc aacgggctgc
     1681 cgctgccggc agggccgctg cgcgagcctg cgcgccggct ggagtccgtg aacgcggtgg
     1741 tcgtcacgca tcgtgctgcg gcggccaccc caccgccgtt cgaggttcgt actccgctgc
     1801 tcgtcgacgc gcatctgtcg ctcgactcgg ccgtgaacct cgtgagcggc gagcgccggc
     1861 cgctgcaggc attcggcgac gcgcagtcgc tgcacgcgct ggccgccatc ggccacccgg
     1921 acgccttttt ccgcgccctg cgcgacgcag ggctccggcc ggtcgagcac gcgctgccgg
     1981 atcacgcggc gctcgacccg caggcgctgc cgttccggcc cgacgccacg gtcctgatga
     2041 ccgagaagga tgccgtaaaa tgtcgggagt tcgggcagcc tggctggtgg tgggtcgacc
     2101 tcgaggtcac ggtcgaccgc accgacgccg agacgctgct cgcgtcgatc cacgagcgca
     2161 ccggcctggc cggcgcggga gccgtccttg gatagcaagc ttctcgacat tctggcgtgc
     2221 ccgctctgca agggtccgtt gcagtggcac cgcgaccagg agtgcctggt ctgtcgcgcc
     2281 gaccgcctgg cgttccccgt gcgcgacgac atcccgatca tgctcgagga cgaggcccgc
     2341 ccgctgaaat ccgacgaccc gctgctcgcc gactgagcaa aggaaaggct cgcgtgggct
     2401 ttcacgtcgt catccccgcg cggcatgcct cgacgcgcct gcctggcaag ccgctgctcg
     2461 acatcggcgg tcggccgatg atccagcgcg tcgtcgagca ggcgagcgca agcggtgcgg
     2521 aacaggtgct cgtcgcgacg gacgacgaac gcatcgtcgc ggccgtgcac gatccgcgca
     2581 ggcactcgca gcgcatcgcg ctcatgaccg atccggacct gccctcgggc accgatcgcg
     2641 tcgcggccgt cgctcgatcg ctcggctgga acgcggccac gatcgtcgtc aacgtgcagg
     2701 gcgacgagcc gttcgtaccg ccggtgctga tcgaccaggt ggcggccctg ctcgagcacg
     2761 acgtgcgcgc ggacgtcgcc acgctcgcgg cgccgatggg cacgctggcg gaattcatgg
     2821 accccaacgt cgtcaaggtc gtcatggcgg ccgacggcag cgcgctgtat ttcagtcgcg
     2881 cgccgatccc gtgggcgcgc gacggcgcgc cgggcggcct caccagccag acgcgtttcg
     2941 acggagccct acgccacgtc ggcttgtacg cttaccgcgt cggcgcgctc gagcgcctga
     3001 cgcggctgtc gccgtcgccg ctggagcagg ttgagaagct cgagcagctg cgcgcgctgc
     3061 aggcgggatt gcgtatcgtc gtcgcccatt gcgccgtgcc cgccgcaccc ggcgtcgaca
     3121 ccgagcacga cctcgaacgc gcgcgggcgc gcgtccatcg cggagccgag ccagcatgaa
     3181 cgagccgttc cacgtcctct ggtttgtcac gctcacgccc tggaaactcg tcggctacct
     3241 gggcgtgacc ctgttcgccg ggcgctggtt cgtccagctc tactactcgc gcaagctcgg
     3301 caggcccgtg gtgccgacgg tgttctggct gatgagcatt tccggcagcc tgctgctgct
     3361 ctcctacttc acgttcggca agaacgattc ggtcggagtc ctctccaacc tgtttccggc
     3421 gggcgtcgcg gcctacaacc tgtacctgga cctcgccaac cggcgcagcc gcagcgcagc
     3481 ggacgcatga cggccgcgca acgccgggat cgcacggcgt cgacgggcgg cctcgtcggg
     3541 acgagccgcg tcagcctttc ttcagcgtga gggtggcccg ccgggcggat cgctcgagcg
     3601 ggagcaacgg ccgcacctcg gcgtccgtca gggggcgcca cttgccgatc ggcaggtcac
     3661 ccaggtggat gtgcatgatg cgtacgcgct gcagccgccg cacggtgtag tcgaagtgtt
     3721 cgcacatccg ccggatctgc ctgttcagtc cctgcgtgag cacgatccgg aacgtgttgc
     3781 ggccgacctg acggaccttg cacgggttcg tgaccgtgcc gaggatcggc acccccgtcg
     3841 ccatgccggc gaggaagtcc gccgtgagcg ggcggtcgac ggagacgatg tactcctttt
     3901 cgtgctcgtg ctcggcgcgc agcacgcggt tgacgatgtc gccgtcgttg gtgagcagga
     3961 tcagcccctc ggactccttg tcgaggcggc cgatcgggaa gatccgctcg cggtgcgcga
     4021 cgaagtcgac gatgttgtcc ggcacgccac gatcggtcgt gcattcgatg ccgatcggct
     4081 tgttgagcgc caggtacacg cgctgcgggc gcgcggcgac gggccggccg tcgagcagca
     4141 cctcgtcgcc atcggcgacc tgcgtgccga gcacggcggt tgcgccgttg acctgcacgc
     4201 gtcccgcctc gatccaggcg tcggcctcgc gtcgcgagca ggcgccggat tcactcagga
     4261 acttgttgag tcgcacgtcg cggactccgc gtgacgcgtt cagccgcccg cgcggcggac
     4321 ccggtagccg agcttgccga gttcctcgac cagcaggtcg cgatggtcgc cctggatctc
     4381 gatcacgccc gcatcgaccg tgccgccgga gccgcagcgc ttcttgagac gggttgcgag
     4441 ttctgcgagc gcatctccgg cgagcggcaa gccgcggatg accgtgacgc ccttgcctct
     4501 gcggccctgc gtctcgcgcc cgacgcgcac gatgccgtcc ccggcaggcg tcacgggctg
     4561 ggccggcttg ctgcggcgac agcggcagtc ctcgacggtt cggccgcact cggggcagcg
     4621 gcggccgacc ccggtcgaat agacgatgcg ttgggaactc atgggcgtgg cgccagttta
     4681 cccgttgccc tcgtaggagt cggcaccgac cccgatggga cgaccgaccg cctcatatgc
     4741 gacggccgcg aaggccgcca tggccccgcg tgaccgcgcc cgcagctatc atccgcccgt
     4801 gctcgaccgg caccaagggt gcaggtcgtc gcggtcgcat cagggaggcg gcatggccac
     4861 gacgcagatc ttcgtcaacc ttccggtgca ggacctggac cggtcccgtc agttcttcgg
     4921 tgccctcggc tatcggtttg acccgcagtt cacgaaccag gacgcggcct gcatgatcgt
     4981 cgaggagggc atctacgtga tgctgctcac cgaaggtttc ttccgcacgt tctgcccggg
     5041 caaggaggtc tgcgataccg gcagcagcgc cgaggtgctg gtgtgcctga cgtgcccgac
     5101 gcgtgagcac gtcgacgacc tggtccgcaa ggcggtcgcg gcgggcggtt cgacaccgcg
     5161 tccgccacag gatcagggct tcatgtacgg gcacgcgtac gaggacctcg acgggcacat
     5221 ctgggagctg atcgcgatga cgccggccga agaaaaggca gccgacactc gatgagcacg
     5281 gcggcggaga caccggcggt gtgcgagacg ccgcgcctgc ggttgcgaca ggtcactgag
     5341 gacgacgccc cgttcatcct cgagctgctc aacgatccgg gctggctccg gttcatcggc
     5401 gatcgcggcg tgcacacgct cgaggacgcg cgcaggtaca tcgagcaggg tccgcgcagg
     5461 atgtatgccg atcacggctt cgggctgctg ctggtggaac gcaagcacga cggcgccgca
     5521 ctcggcctct gtggactgat ccgtcgcgag acgctgcccg acgtcgacct cgggttcgcg
     5581 ctggcggaac ggttccgtgg gcagggctac gcctgcgagg cggcggccgc gacgctgcga
     5641 cacgcgcggg agacgctgaa gctcacgcgg gtggtcgcaa tcgcgatgcc ggagaacgtc
     5701 gcttcgacgc ggctgctcga gcgactcggc ctgcggttcg agcgcacgat cacgttcggt
     5761 ccggcggccg aaaccctcaa ttatttcggc accaccgccg ctttcgcgtt cgacgccgag
     5821 aacgtgcacg ccgtgggcga ccgtctctgg acgtcgggcc agctctcgcg tcgcgacatc
     5881 gagcggctgc ctgctctcgg cgtcgatgcc gtcgtgaacc tggcgccgcc cacgtcctcg
     5941 aatgcgctgc cgggcgaagc cgaggccgtc acggggctcg gcctgacgta cgtgcagatc
     6001 ccggtcccgt gggatcggcc ggacctgcgg caactgcagc agttcttcgg cgtcatggac
     6061 accctggcgg gccgtcacgt ctggatccac tgcgccagga acatgcgcgt gtcggcgttc
     6121 gtctacctct accgccggct gcgacgtggc gagcccgacg cggccgcccg tcatccgatg
     6181 gacgacgtat gggcgccggc agggacctgg cgcgagttca tcgaccgtgc gctcgaggcc
     6241 gcggccgcag gttgacccgt ccaccggacg cataggccgc tacggatcgc accggcgaag
     6301 acccactgcg tttctccatc gcgctcggcg ttcgcaaggg cgacgcggcg ctcaggcggg
     6361 cgctgcagac ggtactcgag tggcgccatc ccgaggtgca gcaactgcgc gccgcgtacc
     6421 acgtgccggt ggtcgccagg ccgccggcgc gcaaggcggc caacgtgagc cgtcaacccg
     6481 gaccggccgg gccgacgcta gaatgaagtc tcgcttccac cggactgccg catgcgcgtg
     6541 ctcttcgtct gcatgggaaa catctgtaga tcgccgacgg ccgagggcgt gttccgggag
     6601 ttcgctcgcc gccatgcgcc ggacctcgag ctcgagatcg attcggccgg gacgcacgac
     6661 tatcacgtcg gcgagccgcc cgacccacgt gcgttgaagg cggcctcgac gcgcggcctg
     6721 gatatcagcg gtctgcgtgc gcgacaggtc gaagcggcgg atttcgagcg cttcgacctg
     6781 atcctcgcga tggaccggct caactacgaa accctgcgcg agcgcagccc cccggcgttc
     6841 cgctcacgca tccggctgct gctcgagttt gccgacgacg cgagccgcga ggacgtgccc
     6901 gatccgtact acggcggccc gaaaggcttc gaggaggtgc tggacctgct cgaagacgcc
     6961 gccgccggcc tgctggccga agtccgccgc aaggcctcgt agcaatccct cgaggagtct
     7021 cgtccaccat gccgagccag gcttcgttcg cacgttcgct ttgcgcctgg ggcgcagccg
     7081 cggcgctgac cgtcgtgtcg ctgcagctga cggtcgcgta cgccgcgacg accggtcccg
     7141 cgtttgctcg ggcgctgcct gagctgccgt tcgaccggat cctgcgctac gacgagatga
     7201 cgcggctgct cgagcagtgg gccgcggcga gaccggcgct cgtgcgactc gaatccatcg
     7261 gccgcacgcc ggaaggtcgc gagctgtggt tcctcacgat cagcaactcc gccacgggtc
     7321 ctgccgccga caagccggcg ctgctcgtcg acggtcacat gcacgcgatc gaaggccccg
     7381 gcaacgtcgc ggcgctgaac ttcgtgtgga cgctgctgcg cgactacggc aaggaccagc
     7441 gggtgacccg cctggtcgac acgcggaccg tctacgtcct gccgcgcctg acgcccgacg
     7501 gcgtcgaagc cgtgctgcgc gacgatcgca tcattcgctc ggcgatccgt cccgcccccg
     7561 cagaagccgc gccggacggc ctcgagatgc gcgatctcga cggcgacggg cggatcgtgt
     7621 tcatgcgcta tcgcgacccg ctcggtccgt ggaaggcgca tccgtccgac gcgcggctgc
     7681 tgattccacg cgcctccgga gaacgggaag gcgagttctg gcgcgtcgta cccgaaggcg
     7741 agatcgaggg ctacgacggc accaacatca ctgtgcagcc cgcgctcgcc ggcatcgacc
     7801 tcggagtgtt cttcccggat ccgcgggacc cggtaccgcc gggttccgcg tccggtccgg
     7861 acccggtgcg cgcgcccgag gccgcggcgt acacggcggc gatccttgct cgaccgaaca
     7921 tcgtcacgca cgtctcctgt cacagcttcg gcggcgccat cctgctgccg ccggtcaacg
     7981 cggacgagga catgccgcag gccgatcgac tggcctacga cgccctcgcc ggccgcggtg
     8041 ccgcgctcac cggatacgac gccatgacgt acctgagcct gcgcggcgga cgcgggctcg
     8101 acgcccacat cccgaccgag atcggctggc tctacaaccg gctcggggtc tacgcgttca
     8161 tcaccgagtt ctggaatccg ctgcgtgccg ccgggatcga actcgaaggg ccgatgtcgt
     8221 tgtggctggg cggcaatcat ccagtcgagg acgacctgaa gctgctgaag tggaacgaca
     8281 ccgaactgca gggacgcggg ttcgtgccct ggcatgcgtt caggcaccgc cagctcggcg
     8341 acgtcgagat cggcggctgg gacaaggtga acttctggta caacgcgccg tacacgcggc
     8401 tgcgcgagga agtgatgccg catgcgcagt ggctcaccga actggcgctg gcctcgccgc
     8461 ggctcgaggt gcgctccgtt tcggcgacgc cggtcggtga ccggcggtgg cgcgttcgcg
     8521 ccgtgctgca gaacacgggc tggctgccga ccagcggctc gcagcgtgcg gtggacgtcg
     8581 aggccgtcgg tgacgtcgag gctgcgctcg agctgccacc cggtgcgcgg ctcgtcgagg
     8641 gcgagcgcgt gcgcgaggcg gggcagctgg ccggactgag cgagcagcgt tcactgtcca
     8701 cgtggtgggg ctatgcgccc ggcaccccag acatcgccac ggtcgactgg ctcgtcgagg
     8761 cggccgacgg ggccacgctc ggcgtggagg cgcgccatcg gcgtgccggg gtggcgcgcg
     8821 cggaggtggt cctgcgcagg ccgtagaggc gccgccggct ccaccctttt gccgctacac
     8881 tgcggatcgc ggcgatcggg tgggggagtg ccgcaggatg ctcccatacg ggccctgtgg
     8941 ccgcgacaac aataaccctc gaacaagggc agcgaatgca gtcggccgct cgagcgacgc
     9001 tactgacagc gttggagcag tatttttcga taccgcgcga agaagccgcg ctcgtcctcg
     9061 acgagttcga gccgagcgtc tgccgcgggg gcgactggtt gttccgtcag ggtgacgccg
     9121 cggattgcct gtacctgctg gcgcgcggcc ggatgcaggt ctggctcggc caggcgagcg
     9181 gcgaccgcga ggaacggctg gtcgccgaag tcaatcccgg cgagacgatc ggcgagatcg
     9241 ggatgctgac cggcggcgcg cgctcggcca gcgtgcgcgc cgtgcgcaac agcttgctgc
     9301 tgcggatgac gtccgcggcc ttcgatcgtc tcgcgcgcca gcgtcccgac ctgacccgtc
     9361 acatcgccgg cagcatcgcc ggccggttgc gtgaccggac ggcaggcgtc tcacacatac
     9421 gccggacgct caagaccatc gcgctgctgc cgctcggccc gatgtcgccg gtcgaggcgc
     9481 tgtcgcagcg actgcgcgac gctctcgcca cctacggatc gacctgcgtg ctgaccccgc
     9541 agacgatgcg cgaggccggc gccccgccgc tgccgacggc ccgtcacgaa ggcttgtcgc
     9601 ccgccatggt cgactggctc gcggtcaagg aggacgagca ccggttcgtg ctctacgtgg
     9661 gtgacccgag cgaagtcttt tccgacatcg cgctgcatca cgcggacctg atcctgctcg
     9721 tggcccaggc gagcgacgac gcggcggttc gcccgtggga acgtgccgcc ttcgaaagcg
     9781 ccgcagcgcc cgtggcgcgc agggcgctcg tgctgtgcca tcccggcgcc gacgagcggc
     9841 ttgaaaacac ggcggcgtgg ctggaggagc gaaaactcga tttccacctg cacgtgcgcg
     9901 atccggtgcg caacgacctc gatcgcctgg cgcgcgtgat cgcgggacga gccatcgggc
     9961 tcgtgttcgg cggcggcgcg gcgcgtgggt ttgcccacct cggcgtctac cgtgcgttgc
    10021 acgaggccgg catcacggtg gactggctcg gcggcgcgag catcggtgcg gtgatggccg
    10081 cgagcctggc gacaggcccg gagcccgccg tcgcgatcga gcgggccagg gtcgccttcg
    10141 tggccggcaa gccgtttggc gacgtcacgc tgccgatcat ttcgttcctg cgcggacggc
    10201 gcatggaacg cctgatcgac gcgaacctgc ccggtcgaat cgaggacctg ccgatcccgt
    10261 tcttctgcgt ctcgagcaac ctcggcaatg gatcggtgca ggtgcacgat cgcggctcgc
    10321 tgccgcgggc cctgcgcgcg agcgtgtcgc tccccggagt cttcccgccc gcggtggtca
    10381 atggccagct cgccgtggac ggagggatcc tcgataacct gcccgtggac ctgatgcgtg
    10441 cacgccccgt gggccgcgtg atcgccgtcg acctgagttc acgcaaggac tacaccgtcg
    10501 actacgactc cgtgccgtcg ccgtggcagg tgctcgcggg tcgtacgttg cctctgtcgc
    10561 ggcgatatcg cgtgcccagc cccgtttcgc tgatgttgat ggcgatgtcg atcggcgcga
    10621 tcggtgcggc gcgcgcggcg ggcgcccg
//
LOCUS       DASPFR010000391         4102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1337456, whole genome shotgun sequence.
ACCESSION   DASPFR010000391 DASPFR010000000
VERSION     DASPFR010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337456"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VD737_10400"
     CDS             <1..691
                     /locus_tag="VD737_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11019.1"
                     /translation="SFYPLMTAPTARHKMEKSMGRNDPSMRHPMYERILERLLGADYH
                     AASAWCFSRNRGMFDEYIIDQADYVGVGSGAFSYVGGAYYSTTFSLNHYCRRIEGGQS
                     GITQRRPVSARDQMRYDYLVRLFGGELRRDYVERRYGAAFWLRMAPELLAMRAIGATR
                     HDPDAIRLTHRGRYCWMLMMAEFFNSVNNVREQMREHIRAELDAWNEEARVPVSAITH
                     AAPPRRSATGS"
     gene            694..1704
                     /gene="hypE"
                     /locus_tag="VD737_10405"
     CDS             694..1704
                     /gene="hypE"
                     /locus_tag="VD737_10405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HXW11020.1"
                     /translation="MQDTHVSLAHGNGGRYMRELIEEIFARHLGNPLLDTDSDAAVLP
                     WDCAQGDLTFTTDSFTVQPIEFPGGNIGSLAVHGTCNDLAVAGAVPRYLSLGVLLEEG
                     LEFAVLERVVAAIAAASREIGVVVAAGDTKVVRRGEGGGIYLNTSGIGVRNRELKLGV
                     REVRAGDAILVSGPVGDHGIAVMLAREQFGLRGDLQSDAASVLPITLALAQVPGLRFM
                     RDPTRGGIATVAHEIVHGSGLGVWLDEARVPVRDSVRSVCEMLGYDPYFLACEGRVVA
                     VVAGEQADAALAAVQALPAGRDAAIIGTVREQPQRVVLRTPLGGERVIEELEDDPLPR
                     IC"
     gene            complement(1818..2186)
                     /locus_tag="VD737_10410"
     CDS             complement(1818..2186)
                     /locus_tag="VD737_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXW11021.1"
                     /translation="MTRNLEWWQGLCATIDAKDTDRFIHHLADGCEFRFGNGPSVIGH
                     EAIGAAVDAFWASIRASEHHVTDCWSGADSAACEGTCTYTRHDGSTITLPFVDVLHFR
                     GDKAVKYCIYMDVGPLYGSV"
     gene            2695..>4102
                     /locus_tag="VD737_10415"
     CDS             2695..>4102
                     /locus_tag="VD737_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11022.1"
                     /translation="MLASVQPESCGTCHAGAGDKHQAIYDDYIDSANKNLELDILGVV
                     TTPVGPNDYTLRMNFAIRDAAGNAIADGDKLPSFMSPGQRTFYVVAYDSANRKFDKLL
                     GFGANPSSGTCTANCSSGVIDDTGGNYHITLTKVAYDPSAPPVPYTGALAYGYVAKAS
                     AVMVNEGHYRLYSDFASDGLVVSGDVSTTPYVSTANVEACEKCHGSPYQKHGYREAQV
                     ANLPDFVACKTCHYDSRPGSDPIFQVMLNDPYDWATGGNGYVNDDESSGQVLPQYQYV
                     ADVMNDTHMSHAMEFPYPQSMANCVTCHEGKLALVINDDTFTAKTCRSCHAVEGIDAW
                     EGQKYDEPRAPALEELWTKANVTFHDVSDDCQVCHKEGGTGSTFGEYHSGYNAMIYDA
                     AGQRYDELYKVAVDEVTFDDTTSVLTVKLSATNTATKPVVTVNFYGYDSKDFYISGHT
                     ADGHVGTDATTACWSSRGN"
BASE COUNT          730 a         1370 c         1355 g          647 t
ORIGIN      
        1 ctcgttctac ccactgatga cggcgcctac ggcgcgtcac aagatggaaa agagcatggg
       61 ccgcaacgac ccttcgatgc ggcacccgat gtacgagcgg atcctggagc gcctgctcgg
      121 cgcggactac cacgcggcgt cggcgtggtg cttctcacgc aaccgcggca tgttcgacga
      181 gtacatcatc gaccaggccg actacgtggg cgtgggcagc ggcgcgttca gttacgtggg
      241 cggcgcctat tactcgacca cgttctcgct gaaccactac tgccggcgca tcgagggcgg
      301 ccagagcggg atcacccagc gacgacccgt gagcgccagg gaccagatgc gctacgacta
      361 cctcgtgcgg ctgttcggcg gtgagctcag gcgcgactac gtcgagcggc gctacggcgc
      421 ggcgttctgg ctgcggatgg ccccggagct gctcgcgatg cgcgccatcg gcgcgacgcg
      481 tcacgacccg gatgcgatcc gcctcacgca tcgcggccgg tactgctgga tgctgatgat
      541 ggcggagttc ttcaactcgg tgaacaacgt gcgcgagcag atgcgcgagc acatccgcgc
      601 cgagctcgac gcctggaacg aggaagcccg cgttcccgtt tcggcgatca cccatgccgc
      661 gccaccgcgc cggtccgcga caggcagctg accatgcagg acacgcacgt ttccctcgcc
      721 cacggcaacg gcgggcgcta catgcgcgag ctgatcgagg agatcttcgc gcgccacctc
      781 ggcaacccgc tgctcgacac ggacagcgac gccgcggtgt tgccgtggga ttgcgcgcag
      841 ggcgacctca cgttcacgac ggacagcttc acggtgcagc cgatcgagtt cccgggcggc
      901 aacatcgggt cgctcgcggt gcacggcacc tgcaacgacc tcgcggtggc cggcgcagtg
      961 ccgagatacc tgagcctcgg cgtgctgctc gaggagggcc tcgagttcgc ggtgctggag
     1021 cgcgtggtcg ccgcgatcgc cgccgcgtcg cgcgagatcg gcgtggtcgt cgcggcgggc
     1081 gacaccaagg tcgtgcgccg tggcgaaggc ggtggcatct acctgaacac gagcggcatc
     1141 ggcgtgcgca accgcgagct caagctcggg gtgcgcgagg tccgcgccgg cgacgccatt
     1201 ctcgtgagcg ggccagtggg cgaccacggc atcgccgtca tgctggcgcg cgagcagttc
     1261 gggctgcgtg gcgacctgca atccgacgcg gcgagcgtgc tgccgatcac gctcgcactc
     1321 gcgcaggtgc cggggctgcg cttcatgcgc gatccgacgc gcggcggcat cgcgaccgtc
     1381 gcgcacgaga tcgtgcacgg cagcgggctc ggcgtctggc tcgacgaagc acgcgtcccg
     1441 gtgcgcgact ccgtgcggtc ggtctgcgag atgctcggct acgacccgta tttcctggcc
     1501 tgcgaaggac gcgtcgtcgc ggtcgttgcg ggcgagcagg ccgatgccgc gctcgcggcc
     1561 gtgcaggctc tgccggccgg gcgcgacgcg gcaatcatcg gcaccgtgcg tgagcaaccg
     1621 cagcgggtcg tgctgcgcac accgctcggc ggcgaacgcg tgatcgagga gctggaggac
     1681 gatccgctgc cgcgcatctg ctgatgcgcg gcagcgcaga gcggttagcg gttagtgagt
     1741 agcggttagt cggagaagat tggcgcagcg cgactgtcga gttctgggcg ttctgactaa
     1801 ccgctaaccg ctaaccgcta gacacttccg tacagcggcc ccacatccat gtagatgcag
     1861 tacttcacgg ccttgtcgcc gcgaaagtgc agcacgtcca cgaacggcag cgtgatcgtc
     1921 gagccgtcgt ggcgggtgta ggtgcaggtg ccttcgcagg ccgcactgtc cgcgcccgac
     1981 cagcaatcgg tcacgtggtg ctcgctcgcg cggatcgacg cccagaacgc gtccaccgcg
     2041 gcgccaatcg cttcgtggcc gatcaccgaa gggccgttgc cgaaccggaa ctcgcaaccg
     2101 tccgcgaggt ggtgaatgaa gcggtccgtg tccttggcgt cgatcgtcgc gcacaggccc
     2161 tgccaccact cgagatttct cgtcatgcaa cgcgctcccg tcgctgtcct tgtttcgacg
     2221 cggtccatgt tgccacagcg tccaatgcgt ttgatgtggt gacatctcga cgcgctcgag
     2281 tgacgccggg tacctcgtcg aatgacgtgc cgtcacctga ctgtgcatta atcgctagtc
     2341 cgaatgggca cgcaatgaca ccgcgcgaaa aaagttctcg gctaatgaca attattgctg
     2401 tgcatcaatc tccctaacat ttttcgccat ctgggctggg catccgtccc gcctcgtggc
     2461 ccgaccgcat cgggtgaggg agtcgcacaa atgagatctg caaaaattcg gagcggccgg
     2521 gctctgctgc cgctcttcac actgttggcc atgggagtcg gcatcgccgg ctgttccggg
     2581 gatgacggca aggacggcgc cgctggcccg accggtggca ccggagcgac cggtcccacc
     2641 ggcccgacgg gtccgacagg accgaccgga ccaggcggga acgacccgac gaacgtgctt
     2701 gcgagtgtcc agccggagtc ctgcggcacc tgtcatgcgg gcgccggtga caagcaccag
     2761 gcgatctacg acgactacat cgacagtgcc aacaagaacc tggagctgga catcctgggc
     2821 gtcgtcacga cgccggtcgg ccccaacgac tacacgctgc ggatgaactt cgcgatccgc
     2881 gacgccgccg gcaacgccat cgccgacggc gacaagctgc cgtcgttcat gagcccgggc
     2941 cagcgcacgt tctacgtcgt tgcgtatgac tccgcaaacc gcaagttcga caagctgctc
     3001 ggcttcggcg cgaatccctc gtccgggacc tgcaccgcga actgctcgag cggcgtgatc
     3061 gacgacacgg gtggcaacta ccacatcacg ctgacgaagg tcgcgtacga cccgagcgca
     3121 ccgccggtcc cgtacaccgg agcgctcgcc tacggctacg tggcaaaagc ctcggcggtc
     3181 atggtcaacg agggccacta tcgcctgtac agcgacttcg cgagcgacgg gctcgtggtc
     3241 tctggcgacg tgtcgaccac gccgtacgtc tcgaccgcca acgtcgaggc ctgcgagaag
     3301 tgccacgggt cgccatacca gaagcacggc taccgtgagg cgcaggtggc gaacctgccc
     3361 gacttcgtcg cgtgcaagac ctgccactac gactcgcgac cgggctccga cccgatcttc
     3421 caggtcatgc tgaacgatcc ctacgactgg gcgacgggcg gcaacggcta cgtcaacgac
     3481 gacgagagca gcggtcaggt gctgccgcag taccagtacg ttgccgacgt gatgaacgac
     3541 acgcacatgt cgcatgcgat ggaattcccg tatccgcagt cgatggccaa ctgcgtcacc
     3601 tgccacgaag gcaagctggc gctggtcatc aacgacgata cgttcaccgc gaagacctgc
     3661 cgcagctgcc atgcggtcga aggcatcgat gcgtgggagg gccagaagta cgacgaaccg
     3721 cgcgcccccg cgctcgagga gctttggacg aaggcgaacg tcactttcca cgacgtctcc
     3781 gacgactgcc aggtctgcca caaggagggc ggcaccggca gcaccttcgg cgagtatcac
     3841 agcggctaca acgcgatgat ctacgacgcc gcgggccagc ggtatgacga gctgtacaag
     3901 gtcgccgtcg acgaagtcac cttcgacgac acgaccagcg tgctgacggt caagctgagc
     3961 gccaccaaca cagcgacgaa gccggtcgtg accgtgaact tctacgggta tgactccaag
     4021 gacttctaca tcagcggcca tacggccgac ggtcacgtgg gcacggacgc cacgactgcg
     4081 tgctggagct cgcgtggcaa cc
//
LOCUS       DASPFR010000392         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_969915, whole genome shotgun sequence.
ACCESSION   DASPFR010000392 DASPFR010000000
VERSION     DASPFR010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969915"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..694)
                     /locus_tag="VD737_10420"
     CDS             complement(131..694)
                     /locus_tag="VD737_10420"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HXW11023.1"
                     /translation="MSKRSAATAILIALATTPGIAAAENDSGLYLGAGVGRFNVEIDD
                     VEDVTDTIGSFDADDTTFKAFAGWRFNPYLAVELDYIDLGNPEDRIDGRRVSADVNGF
                     APYVVGTLPVGPVELFAKAGYLFYDVKVDVDDLDIEDDSNEDFVYGGGIGVTMFEHLH
                     ARLEYEVIDLGEVDDANALWLSAAWRF"
     gene            931..3060
                     /locus_tag="VD737_10425"
     CDS             931..3060
                     /locus_tag="VD737_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXW11024.1"
                     /translation="MTPITSKSCRLRIAIALTLSPLLALSDEATTEPTVAEQAPALET
                     VTVTAQKVTQDLQKTAAAVTALSGEALVSAGAYDIRAVQNLMPSVRFQAEGASTEIYI
                     RGVGSTLDLPNIEPPTVFNFNGVYIPREGTSVGLFDIASVELLPGPQGTLYGRAALGG
                     TVNVDFNRPTDEFETSGTIEAGNHSLLHGTIVQNVPVTEAFALRGAFDYVEQDGYLRT
                     GADSRQDYAARLSGLYAPSDDVDLYVWIHGAKKDGESPNLVRRGFNGGTFDGNPNAYD
                     HDDPWNDRIDPGAPTASPNDYESLGAGAQLDWRRDGYTLTYLPSYFHLDWDGRYWLEN
                     IGAQLTARYDQVTQELRIAGESDTRLRWLGGLYYYRVSNDGDFYTYTNTPAPFPLAQI
                     EQNRLEGYAAFGQATWDLSATTRLVAGGRFSADQREGRGETADGRPYSADEDYDHFDW
                     KVGVEMDFGASAMGYANVQTGYQPGTYNMFPATAEASNLVRSATMTAYTVGFKSRFFD
                     DRLQVNDEVFYYDYEDLLVQSFNFNTFLLTTFNAAESRIYGNQLDVLFQATGRDRFNL
                     TVGYLDAQYDVFVVPDEVDIGLPRRDFGGYPLQYAPEWTVTAGYQHDFAVGAATLRAR
                     VETRYEDEFWGTFAQNRGTQQPDYFKTDASLTYFAPDDRWSVGLWVRNLENVAVLAAT
                     TTGQFGPYGDAFIEPPRTYGARFTFRL"
     gene            complement(3088..>3323)
                     /locus_tag="VD737_10430"
     CDS             complement(3088..>3323)
                     /locus_tag="VD737_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXW11025.1"
                     /translation="VLLDLGLPDMPGEQVAQRLRQGTLARDARLIAITGWGQEADRAR
                     TREAGFDLHLVKPVDPDVLLRHVGERMDATQSG"
BASE COUNT          569 a         1150 c         1077 g          527 t
ORIGIN      
        1 cgtagctcag ttggatagag catcaggctt cgaacctgag ggtcgggggt tcgaatccct
       61 ccggccgcgc cagcttagga aaagggccac ctcgcggtgg ccctcttcac atcggccggg
      121 aatgcgatcg tcagaaccgc caggcagccg acagccacag cgcgttggcg tcatcgacct
      181 cgccgaggtc gatgacttcg tattccagcc gcgcgtgcag gtgctcgaac atcgtcacgc
      241 cgatgccgcc gccgtacacg aagtcctcgt tgctgtcgtc ctcgatgtcg aggtcgtcga
      301 cgtcgacctt gacgtcgtag aacaggtagc ccgccttcgc gaacagctcg accgggccga
      361 ccggcagcgt gcccaccacg tacggcgcga aaccgttcac gtcggcgctg acccgcctgc
      421 cgtcgatgcg gtcctcggga ttgccgaggt cgatgtagtc cagttcgacg gccaggtacg
      481 ggttgaagcg ccaccccgca aatgccttga acgtggtgtc gtcggcgtcg aacgagccga
      541 tcgtgtccgt gacgtcctcg acgtcgtcga tctcgacgtt gaaccggccg acgccggcgc
      601 caaggtacaa gccgctgtcg ttctcggcgg cggcaatgcc gggcgtcgtg gcgagcgcga
      661 tcaggatcgc cgtggctgcg cttcgtttgc tcatgcgtga cctcacgtgg tcgtgaaaca
      721 tcgcggctgt ataagttcac gaccaccgca ccggtatccg cccccgccgt caggtgaatc
      781 ccacttcggc cccgtgatgg gtaaactgtg cgcaagcggt gcgggccgcg gacgtcgaag
      841 ccgaggattt ccgggccgct ccatcccagt gccaacgctc ctcaggcgcg gtccgacagt
      901 cgcgccccac ctgtccgtga accatccgag atgacaccaa tcacatctaa gtcctgccgg
      961 ctgcgcatcg ccatcgccct cacgctctcg cccctgctgg ccttgagcga tgaggcgacg
     1021 acggagccga ccgtggcgga gcaggcaccc gccctcgaga cggtgaccgt cacggcccag
     1081 aaggtgacgc aggacctgca gaagacggcc gccgcagtga ccgcgctctc gggagaggcg
     1141 ctggtgagcg ccggcgccta cgacatccgc gcggtgcaga acctcatgcc ctcggtgcgt
     1201 ttccaggccg aaggcgcttc gaccgagatc tacatccggg gcgtcggttc gacgctcgac
     1261 ctgcccaaca tcgagccgcc gacggttttc aacttcaacg gcgtctacat cccgcgcgag
     1321 ggcaccagcg tcggcctgtt cgacatcgcg agcgtcgaac tgctgccggg gccgcaaggc
     1381 acgctgtacg gtcgtgcggc cctcggcggc acggtcaacg tcgacttcaa ccggccgacc
     1441 gacgagttcg agacgagcgg gacgatcgag gccggcaacc attccctgct gcacggcacg
     1501 atcgtgcaga acgtgcccgt gacggaggcg ttcgcgctcc gcggtgcctt cgactacgtc
     1561 gagcaggacg ggtacctgcg taccggcgcg gactcccgcc aggactatgc cgcgcgactt
     1621 tcggggctgt acgccccgag cgacgacgtc gacctctacg tctggatcca cggcgcgaag
     1681 aaggacggcg aatcgccgaa cctggtgcgt cgcggcttca atggcggtac gttcgacggc
     1741 aatccgaacg cgtacgacca cgacgatccg tggaacgacc gcatcgaccc gggtgcgccc
     1801 accgcttcgc ccaacgacta cgagagcctg ggcgcgggcg cgcaactcga ctggcgacgt
     1861 gacggctaca cgctcaccta cctgccgtcg tatttccacc tcgactggga cggccgttac
     1921 tggctcgaga acatcggcgc ccagctgacc gcccgctacg accaggtcac gcaggaactg
     1981 cgcatcgccg gcgagtcgga cacgcgtctg cgctggctcg gcgggctcta ctactaccgc
     2041 gtgtcgaacg acggcgactt ctacacgtac accaacacgc cggcgccgtt tccgctggcg
     2101 cagatcgagc agaaccgact cgaggggtat gccgcgttcg ggcaggcgac ctgggacctc
     2161 tccgcgacga cgcgcctggt cgcgggcggg agattcagcg cggaccagcg cgaaggccgc
     2221 ggcgagaccg ccgacgggcg gccgtactcc gcggacgagg actacgatca cttcgactgg
     2281 aaagtcgggg tggagatgga tttcggcgcc agtgcgatgg gctacgcgaa cgtgcagacc
     2341 ggctaccagc cggggacgta caacatgttc ccggcgaccg cggaggcgtc gaacctggtc
     2401 cgctcggcga ccatgacggc ctacacggtc ggattcaagt cgcgtttctt cgacgaccgg
     2461 ttgcaggtca acgacgaggt cttctactac gactacgagg acctgctcgt acagtcgttc
     2521 aacttcaaca cgttcctgct gacgacgttc aacgcggccg aaagccgcat ctacggcaac
     2581 cagctcgacg tgctgttcca ggcgacgggc cgcgaccgtt tcaacctgac ggtcggctac
     2641 ctcgacgccc agtacgatgt gttcgtggtg cccgacgaag tcgacatcgg cctgccgcgc
     2701 cgcgacttcg gcggttatcc gctgcagtac gcgccagagt ggacggtgac ggccggctac
     2761 cagcacgact tcgccgtcgg cgcggcgact ctgcgcgcac gagtcgaaac gcgctacgag
     2821 gacgagttct ggggcacgtt cgcgcagaac cgcgggacgc agcagccgga ctacttcaag
     2881 accgacgcat cgctcacgta tttcgcgcca gacgaccgct ggagcgtcgg actctgggtc
     2941 cgcaacctcg agaacgtcgc ggtgctcgcg gcgacgacca cggggcagtt cggtccgtac
     3001 ggggacgcgt tcatcgagcc gccgcgcacg tacggcgcac gattcacttt ccggctctga
     3061 tcgcgcctgc aaagcgcgca cggggcctca gcccgactgc gtcgcgtcca tgcgctcgcc
     3121 gacgtgacgc agcagcacgt ccgggtccac gggcttgacc aggtgcaggt cgaaacccgc
     3181 ctcgcgcgtg cgggccctgt ccgcctcctg cccccagccc gtgatcgcga tcagccgcgc
     3241 gtcgcgcgcg agcgtgccct gtcgcaggcg ctgcgcgact tgctcgccgg gcatgtcggg
     3301 gagcccgagg tcgagcagca cga
//
LOCUS       DASPFR010000393         9217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1458471, whole genome shotgun sequence.
ACCESSION   DASPFR010000393 DASPFR010000000
VERSION     DASPFR010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9217
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1458471"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1368
                     /locus_tag="VD737_10435"
     CDS             229..1368
                     /locus_tag="VD737_10435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXW11026.1"
                     /translation="MLHEFLTANRADLIERCRVKTCQRATPRAAHDEVMHGVPSFLDQ
                     LIRTLQVEQTSEPLQSRRISGPSGGGPAASEIAATAVLDGREFSRQGWSVEQVVHHYG
                     DLCQAITDLAFERNEPIGTDEFRTLNRCLDNGIADAVTEFSFQRSALLQTSAVHALNE
                     RLGFLAHELRNHIHTATLATKAIKAGRVGMSGATGAVLDRSLIGMRDLIDRSLADVRV
                     AAGMEPLHRLVALADFIADIGISASLEAQARGCVLTVGTVDAGLAIDVDREMLFSAVN
                     NLLQNAFKFTHSASEVTLNAYATAARIRIDIEDQCGGLPDGVVDQIFLPFKQGGDDRS
                     GLGLGLAICRRGVEANNGVLKVRDLPGTGCVFTIDLPRHMIPPVQ"
     gene            1546..2247
                     /locus_tag="VD737_10440"
     CDS             1546..2247
                     /locus_tag="VD737_10440"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HXW11027.1"
                     /translation="MVILTGAGVSAASGLRTFRGPGGIWKQHDVERLGHVDALLKRPA
                     ETWTLFGSMREPVRAARPNAAHVALAQWEAELAPDHEFVLVTQNVDGLHQAAGSRNVC
                     EIHGNVMYTRCTNAGCTLDRFRDEDAHAREVPVCPLCAAPLRPDVVLFGEDLPARAAW
                     TARKALRDCDLFIAVGTSGTVSPAAGFVRGAEYAGARTILVNLEPMLERNPAFQEEYL
                     GKAEDVLPELLGLAS"
     gene            complement(2706..2846)
                     /locus_tag="VD737_10445"
     CDS             complement(2706..2846)
                     /locus_tag="VD737_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11028.1"
                     /translation="MVDDGFDRDARMSLRHRAFWTIVGAVSTVLLYLTIVCALPRGAV
                     GY"
     gene            complement(2974..4479)
                     /gene="glpD"
                     /locus_tag="VD737_10450"
     CDS             complement(2974..4479)
                     /gene="glpD"
                     /locus_tag="VD737_10450"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HXW11029.1"
                     /translation="MTTQRPERATSCDVLVVGGGINGAGIARDAAGRGLRTVLCEQHD
                     LASHTSSASTKLVHGGLRYLEFYDFGLVRKSLREREVVLASAPHLAWPMRFVLPHDQH
                     LRPAWMIRAGLLLYDHLAVRRHLPGSETVDLRRHPAGAALDGRYRTAFVYSDGWVDDA
                     RLVVANAIDARERGGRVATQTRCTKLRASGQSWTATLRHEDGRTEQVKARCVVNAAGP
                     WVAQFLDECTPRPARHHPRMIQGSHIVVRRLFEHEYAYLFQNADGRVVFAIPFLDEFT
                     LVGTTEREHEGDLATAEIAPGEVDYLLSTVERHFGRKLARRDVVWTFAGIRPLLAPST
                     QDPKSVTRDYVLDFDRRGPPLLSIYGGKLTTYRKLAEDVVDRVCATLGERARAWTERV
                     PLPGGDMPDGDFEAFRRRMVRRYAWLERPLLDRYSRAYGTRVDRLLEGCSSLADLGRF
                     VLPGLAEREIEYLRREEFATHADDILFRRSKLGVSLPAGSAARLDRWLQKA"
     gene            complement(4476..5684)
                     /locus_tag="VD737_10455"
     CDS             complement(4476..5684)
                     /locus_tag="VD737_10455"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HXW11030.1"
                     /translation="MKSGIDRLLDEPALRRDLRGRRVALLAHPASVTTDLTHTLDALA
                     SLGEDERLTAALGPQHGLRGDKQDNMVESDDYLDPALGIPVFSLYGEVRRPTPAMLDT
                     FDVLLVDLQDLGCRIYTFITTLRYVLEAAAAHGKSVWVLDRPNPIGRPVEGLTLRAGW
                     ESFVGAGAMPMRHGLTLGELGHWFLRRLGLDVDYRVVEMQGWQPDAPPGFGWPLGARA
                     WVNPSPNAPNLWMARCYPGTVMLEGTTLSEGRGTTRPLEVFGAPDLDVPRLLRDMEQL
                     APQWLRGCRLRPCWFEPTFHKHVRQLCAGVQVHVEDGAYDHAAFRPWRLQALAFKALR
                     RQRPDYELWRDFPYEYEHERLAIDLINGGESLRHWVDDDDASPADLDALASADERAWL
                     EQRADVLIYR"
     gene            complement(5681..6295)
                     /locus_tag="VD737_10460"
     CDS             complement(5681..6295)
                     /locus_tag="VD737_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HXW11031.1"
                     /translation="MRPPKPQTVALACHPATSTTAVRGVTASARIGGAGKLAVTFTLD
                     ADMSRVRLPRTRTPARADELWRHTCFEVFVALPESEAYCELNFSPSGEWAMYGFVGYR
                     RGMTPLEVRRPPRIAVRPMPRGLVLEAITGLDELPTPRPGSPLRAGAAAVIEETDGRL
                     TYWALAHPAERPDFHHRLGFVLQVGRTADGVTDILRDAVGNPAA"
     gene            complement(6320..7405)
                     /locus_tag="VD737_10465"
     CDS             complement(6320..7405)
                     /locus_tag="VD737_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11032.1"
                     /translation="MRHHDRPFELRAGPGALRHLRERGLRPGDVCCVPAAAGGPKGLA
                     LLPLDRLLWQEGWLPTDAPVELIGASIGAWRMAALAQPEPLAALDRLQHAYVHGQCYT
                     PRPGPAEISTACRQIARAVHGDGALQVRDCVSLGIVTSRARGPLHDRESKLAFGRAVL
                     SNAISRRRLAGYLERVVFHGGERPGLLDGATAFDAFGLVNVALTDVNAEDALLASGTI
                     PLLASPVRNIAGAPPGLYWDGALVDYHLLLPYQRLTRNGARIVLYPHFNEYVTPGWLD
                     KHLPWRRAPRAHAWLDDVLLVAPSRSFLRTLPNGKLPDRQDFYDYGADHAGRIRAWET
                     AIGQCKAFAEAVLRWMERPDPTLVEPI"
     gene            7525..8172
                     /locus_tag="VD737_10470"
     CDS             7525..8172
                     /locus_tag="VD737_10470"
                     /inference="COORDINATES: protein motif:HMM:NF019559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HXW11033.1"
                     /translation="MGPGPWILLCALAVAGLLVAERRRSQRGKWIAKPLASVAFVATA
                     IASHALESVYGWLVLLALALCLLGDVLLIPTDRAHVFRAGVFAFLAGHVAYAAAFLTQ
                     DTNLTWLAIGAAALVLVLAVVWRWLGPALPDAMRGAVLAYLVVIGAMTALAIGCTGGG
                     GPPTLALGAVAFTASDVSVARDRFVREDFFNRAWGLPLYYCAQLLLALSPAAVAS"
     gene            8172..8879
                     /locus_tag="VD737_10475"
     CDS             8172..8879
                     /locus_tag="VD737_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11034.1"
                     /translation="MGTCLQSSEQLPEAPRAPAPWSLRGHGWIVLLRLPRTAPARTAF
                     VPAALQASLRAAISALVCVDYVSAPCGAYRELLLIPGTMRFPDGRRYASISRILVSTW
                     ASVANGRANWGIPKDRADSFEIERGAASERFRVADGGRELCRLDFEPPRGPRLPLRTA
                     WVPARWGTLAQLHGQRAYYYRPAARGSLRPVRLAHARYDGTLFPDLSVATVLAAFRVE
                     EFTMEFPVARVEVVAAT"
BASE COUNT         1406 a         3159 c         3241 g         1411 t
ORIGIN      
        1 gaacaaccac ctgcgtgctt ctcggtgacg aacacaggac gaaccgttgg cgatgattct
       61 cccgcgcacc gcggacctgg gctacggcgt gatgcgcgcg cccgcgagac gtcaggctcc
      121 agtcgtcggc caccgtgcgc agcgagccgg taccgccgcc cgcccgcctg ggccggcagt
      181 gtccgctacc gcacagacag cgaagcggag tgaggatatg gtcgcctggt gctgcacgaa
      241 tttctcactg ccaatcgcgc tgacctgatc gaacggtgtc gggtcaagac gtgtcaacgc
      301 gcgacgccac gggcagcgca tgacgaagtc atgcatggcg taccgagctt ccttgaccaa
      361 ctgatcagga cgctgcaggt ggaacaaacg tcggaacccc tgcagagccg caggatttcc
      421 ggcccctcgg ggggtggacc cgctgcgtcg gaaatcgccg caaccgcggt tctggatgga
      481 cgtgagttct cacggcaggg ctggtcggtg gagcaggtcg tccaccacta cggcgacctc
      541 tgccaggcca ttaccgacct ggcgttcgag cggaacgaac cgatcgggac agacgagttc
      601 cgaaccctca atcgctgcct cgacaacggt attgccgatg ccgtcaccga gttctcgttt
      661 cagcgcagcg cactgctgca aaccagtgcc gtacacgccc tgaacgaacg gcttggattt
      721 ctcgcgcacg agctgcgcaa tcacattcac accgcgaccc ttgcgaccaa ggccatcaag
      781 gccggtcgcg tgggcatgag cggcgccacc ggggccgtgc tggaccgctc cttgatcgga
      841 atgcgcgatc tcatcgatcg gtccctcgcc gacgtccgag tggccgctgg aatggagccg
      901 ctgcaccgtc tggtcgctct tgccgatttc atcgccgaca tcgggatatc cgcctcgctc
      961 gaggcgcagg cgcgaggctg cgtgctcacc gtcggcactg tcgacgccgg gcttgcaatc
     1021 gatgtcgatc gagaaatgct gttctctgca gtcaacaacc tgctgcagaa cgccttcaag
     1081 ttcacgcaca gcgcgagcga agtcacgctc aacgcctacg cgaccgcggc acgcatcagg
     1141 atcgacatcg aggatcaatg cggcgggctg ccggatggcg tcgtggatca gatcttcctg
     1201 ccattcaagc agggcggcga cgacagatcg ggactgggac tcgggctggc gatctgtcga
     1261 cgcggcgtcg aagcgaataa tggagtcctg aaggtccgcg acctgccggg caccgggtgt
     1321 gtgttcacga ttgacctgcc gcggcacatg atcccgccgg tgcaataagg ggcgttcaag
     1381 tctggacgtc ggccagctgg accggttgac tcgttagcgc gcgagcggct gtcggcgcgc
     1441 ctctcgtgcg agatcccgag cgtagaatct cggacacatg gtcagacaac gtccgagctc
     1501 gctgtggccc ctgccgtgcg aatagaccgc catcggtatc gccgcgtggt catactcacc
     1561 ggtgcgggcg tgtctgcggc ctcgggactg cgcacttttc ggggcccggg tggaatctgg
     1621 aagcagcacg acgtcgaacg gctcggccac gtcgacgcgt tgctcaagcg cccagcggag
     1681 acctggacgc tgttcggcag catgcgggag ccggttcgag cggcgaggcc caacgccgcc
     1741 cacgtggcgc tcgcgcagtg ggaggcggaa ctcgcgccgg atcacgagtt cgtgctcgtg
     1801 acgcaaaacg tcgacggcct gcatcaggcg gcgggcagtc gtaacgtctg cgagattcac
     1861 ggcaacgtca tgtacacccg ctgcaccaac gcggggtgca cgctcgatcg cttccgggac
     1921 gaggacgcgc atgcgcgcga agtccccgtg tgcccgctct gtgcggcgcc gcttcgaccg
     1981 gacgtggtgc tgttcggtga ggacctgccg gcgcgcgccg cctggacggc caggaaagcg
     2041 cttcgcgact gtgacctctt catcgccgtc ggcacctcgg gcacggtgtc tcccgccgca
     2101 ggcttcgtgc gaggcgccga gtacgcgggc gcccgcacga tcctcgtcaa cctggagccc
     2161 atgctcgaac gcaacccggc gttccaggag gagtatctgg gcaaggccga agacgtgctg
     2221 ccggagctgc tcggtcttgc ctcgtagcgc ctggcgcacg acggcttgct ccaaaggttg
     2281 tcgggccaca aggagtgcag aacgatgctc gcgggttcat gccactgcgg tgcggtccag
     2341 atccacgttc ccaggaagcc gcggcggctt acgagctgca actgttcgat ctgtcgccgc
     2401 caggccgggc tttggggtta ctacgagtgc agccaggtga cgatcaccgc gcgcaaggga
     2461 gccatcgacc gctacgaatg gggtgacaag tgcctttcgt tgtgtagatg cgcgaagtgt
     2521 ggatgcgtaa cgcactggct gccagtcgcg cggggcggga aacggatggg ggtcaacttt
     2581 cgcaacttcg atccgtcggc catcgagaca acccggatcc gtaggtttga tggcgccagg
     2641 acctggaaat tcctggactg acgacgaacc ctgctcgcgc gtgcccgggc gctcagcgcc
     2701 tcgagtcagt agccgaccgc tccccgcggc agcgcacaga cgatcgtcag gtagagcagc
     2761 accgtcgaca cggcgcccac gatcgtccag aacgcgcggt ggcgcaggct catgcgtgcg
     2821 tcacgatcga agccgtcgtc aaccacgtgc gtgcactccc cggatcgtgg ggagccatcc
     2881 tagggatgtc ggccaggcgc caccatcgcc aaacgtcgca attccagcca cccgccatag
     2941 cggatgtccg aagcggaccg tgggacgggg atttcaggct ttctggagcc agcgatccag
     3001 tcgcgcggcg ctgcctgccg gcaggctcac ccccagcttg gagcgccgga acaggatgtc
     3061 gtcggcgtgc gtggcgaact cctcgcgcct caggtactcg atctcccgct cggcgagtcc
     3121 cggcaggacg aatcggccga ggtcggcgag gctcgaacac ccctcgagca ggcggtcgac
     3181 gcgggtgccg taagcgcgcg agtatcggtc cagcagcggc cgctcgagcc aggcgtagcg
     3241 ccgcaccatg cgacgccgga acgcttcgaa gtctccatcc ggcatgtctc cgcccggcaa
     3301 tgggacgcgc tccgtccacg cgcgcgcacg ctcgccgagc gtcgcgcaga cgcggtccac
     3361 gacgtcctcg gccagcttgc gataggtcgt gagcttgccg ccgtagatcg acagcagcgg
     3421 cggcccgcga cggtcgaagt ccagcacgta gtcgcgcgtg acgctttttg gatcctgcgt
     3481 cgacggcgcg agcagcggcc ggatgccggc aaacgtccag acgacgtcgc gccgcgcgag
     3541 cttgcgtccg aaatgccgct cgaccgtcga gagcaggtaa tcgacctcgc cgggcgcgat
     3601 ctcggccgtg gccaggtcac cctcgtgttc gcgctcggtc gtgccgacca gcgtgaactc
     3661 gtcgaggaac gggatggcga acaccacccg gccgtcggcg ttctggaaca ggtacgcgta
     3721 ctcgtgctcg aacaggcggc gcaccacgat gtggctgccc tggatcatgc gcggatggtg
     3781 ccgcgccggg cgcggcgtgc actcgtcgag gaactgcgcg acccagggac ctgcggcgtt
     3841 gacgacgcat cgcgccttga cctgctcggt ccgtccgtct tcgtgccgca gcgtcgccgt
     3901 ccaggactgt cccgacgcgc gcaacttcgt gcaccgggtc tgcgtcgcga cgcgaccgcc
     3961 gcgttcgcgc gcgtcgatcg cgttggcgac cacgagccgc gcgtcgtcca cccaaccgtc
     4021 ggaatagacg aacgccgtcc ggtagcgtcc gtcgagcgcc gccccggcag gatgacggcg
     4081 caggtccacg gtttccgagc ccggcaggtg acgtcgcacg gcgaggtggt cgtacagcag
     4141 caggcccgcg cggatcatcc aggcagggcg caggtgctgg tcgtggggca gcacgaagcg
     4201 catcggccag gccaggtgcg gcgccgaggc gagcaccacc tcgcgctcgc gcagcgactt
     4261 gcgcacgagc ccgaagtcgt agaactcgag gtatcgcagg ccgccgtgga ccagcttggt
     4321 gctggcggac gaggtgtgcg acgcgaggtc gtgctgttcg cagagcaccg tgcgcaggcc
     4381 gcgtcccgcg gcgtcgcgcg cgatcccggc gccgttgatg ccgccgccca cgaccagcac
     4441 gtcgcagctg gtggcacgtt ccggacgctg cgtcgtcacc gatagatcaa cacgtcggcg
     4501 cgctgctcga gccaggcccg ttcgtcggca ctcgcgagcg cgtcgaggtc cgccggactg
     4561 gcgtcgtcgt cgtccaccca gtgccgcagc gactcgccgc cgttgatcag gtcgatcgcg
     4621 agccgctcat gctcgtactc gtacgggaag tcgcgccaca actcgtagtc cggtcgctgg
     4681 cgccgcagcg ccttgaacgc gagtgcctgc agtcgccacg ggcggaacgc cgcgtggtcg
     4741 taagcgccgt cttcgacgtg cacctgcacg cccgcgcaga gctgccggac gtgcttgtgg
     4801 aaagtcggct cgaaccagca cggccgcagc cggcagccgc gcagccactg cggcgcgagc
     4861 tgctccatgt cccgcagcag cctcggcacg tcgaggtcgg gcgcaccgaa gacctcgagc
     4921 ggccgcgtcg tgccacgccc ctcggacagc gtcgtgcctt caagcatgac cgtccccgga
     4981 tagcagcggg ccatccagag gttgggcgcg ttcgggctcg ggttcaccca cgcgcgcgcg
     5041 ccgagcggcc agccgaatcc cggcggcgcg tcgggctgcc agccctgcat ctcgaccacg
     5101 cggtaatcga cgtcgagacc gaggcgccgc aggaaccagt ggcccagctc gccgagcgtg
     5161 agtccgtgac gcatcggcat cgcacccgcg cccacgaagc tttcccagcc ggcgcgcagc
     5221 gtcagcccct cgaccgggcg tccgatcggg ttcggacggt cgagcaccca caccgacttg
     5281 ccgtgggcag cggcggcctc cagcacgtac cgcagcgtgg tgatgaacgt gtagatgcgg
     5341 cagccgaggt cctgcaggtc gaccagcagc acgtcgaagg tgtcgagcat ggcgggcgtc
     5401 ggtcggcgca cctcgccata caggctgaag acgggaatgc cgagcgcggg atcgaggtag
     5461 tcgtccgact cgaccatgtt gtcctgcttg tcaccgcgca gcccgtgctg tggaccgagc
     5521 gccgcggtga gccgctcgtc ctcgcccagc gacgcgagcg cgtcgagcgt gtgcgtgagg
     5581 tccgtggtga ccgatgccgg gtgggccagc agcgcgacgc gtcgcccgcg caggtcgcga
     5641 cgcagtgcag gttcgtcgag gagacggtcg atgccggact tcacgcggcc ggattcccga
     5701 ccgcgtcgcg caggatgtcc gtgacgccgt ccgccgtgcg gccgacctgc agcacgaacc
     5761 ccagccgatg atgaaaatcc gggcgttcgg cagggtgcgc gagcgcccag tacgtcaggc
     5821 gcccgtcggt ctcctcgatc acggcggcgg caccggcacg cagcggcgag cccggtcgcg
     5881 gcgtcggcag ttcgtcgagc ccggtgatgg cctcgagcac gagcccgcgc ggcatcggcc
     5941 gcacggcgat gcgcggtggc cggcgcacct cgagcggcgt catgccacgg cggtatccga
     6001 cgaacccgta catggcccac tcgcccgagg gggagaagtt gagctcgcaa tacgcttcgc
     6061 tttcgggaag cgcgacgaag acctcgaagc acgtgtgccg ccacaactcg tcggcacgcg
     6121 ccggcgtgcg ggtgcgcggc agtcgcacgc gggacatgtc ggcatcgagc gtgaacgtga
     6181 cggcgagctt gcctgcgccg ccgatccggg cgctcgcggt gacgccccgc acggccgtgg
     6241 tcgaggtggc cgggtggcag gcgagggcga cggtctgtgg cttcggaggg cgcatcgcgg
     6301 cgattctagc cacccgccgt cagattggct cgacgagcgt tggatccggc cgctccatcc
     6361 agcgcagcac ggcctccgca aaggccttgc attgcccgat cgcggtctcc caggcgcgga
     6421 tgcgaccggc gtggtcggcg ccgtagtcat agaagtcctg ccggtcgggc agcttgccgt
     6481 tcggcaaggt acgcaggaac gagcgcgagg gtgccaccag caggacgtcg tcgagccacg
     6541 catgcgcccg gggcgccctg cgccagggca ggtgcttgtc gagccagcca ggcgtcacgt
     6601 actcgttgaa gtgcgggtag agcacgatcc tggcgccgtt gcgcgtgagc cgctggtacg
     6661 gcagcaacag gtggtagtcg accagcgcgc cgtcccagta cagtccgggc ggcgcgccgg
     6721 cgatgttgcg gacgggggag gccagcaggg ggatggtgcc ggaggcgagc agggcgtctt
     6781 cggcattgac gtcggtcagc gccacgttga ccagcccgaa cgcgtcgaag gcggtcgcgc
     6841 cgtcgagcag gcccggccgt tcgccgccgt gaaacacgac gcgttcgagg tagcccgcga
     6901 gcctgcggcg cgagatcgcg ttgctgagca cggcgcggcc gaaggccagt ttggattcac
     6961 gatcgtgcag cggtccccgc gcgcgggagg tgacgatgcc gagcgaaacg cagtcacgga
     7021 cctgcaacgc tccgtcgccg tgcacggctc gcgcgatctg gcggcacgcc gtgctgattt
     7081 ccgccggacc aggtcgcggc gtgtagcact ggccgtggac gtacgcgtgc tgcaggcggt
     7141 cgagcgccgc gagcggctca ggctgcgcga gcgccgccat gcgccacgcg ccgatcgacg
     7201 cgccgatcag ttccacgggc gcgtcggtgg gcaaccagcc ttcctgccac agcaaccgat
     7261 ccagcggcag caaggcaagg cccttcggcc cgccggccgc cgcgggcacg cagcagacgt
     7321 cgcccggccg caatccccgt tcacgcaggt gtcgcaaggc gccggggccg gcgcgcagct
     7381 cgaaggggcg gtcgtggtgc ctcatgcggc cggcgatggt agggcctccc ggcggccgtg
     7441 ccaatgcacg cggcccggca ggcagtgtca cgccgcgtcc gccatcgtag gctacgatcc
     7501 tgcgaccgac aggggaggcg atcgttggga ccgggcccgt ggatcctgct ttgcgcgctg
     7561 gccgtggcgg gactgctcgt ggccgagcgc cgtcgctcgc agcgcggcaa gtggattgcc
     7621 aagccgcttg cctcggtcgc gttcgtcgct accgccatcg cctcccacgc cctggagagc
     7681 gtctatggct ggctcgtgct gctggcgctc gcgctgtgcc tgctcggcga cgtgctgctg
     7741 atcccgaccg atcgcgcgca cgtctttcgt gccggcgtgt tcgcgttcct ggccggtcac
     7801 gtggcctacg ccgccgcctt cctgacgcag gacacgaacc tcacgtggct cgcgatcggc
     7861 gccgcggcgc tcgtgctcgt gctcgccgtg gtgtggcgct ggttgggtcc tgcgctgccc
     7921 gacgcgatgc gcggggcggt gctcgcgtac ctggtcgtga tcggcgcgat gaccgcgctc
     7981 gcgatcggct gcaccggtgg cggcggaccg ccgaccctgg cgctcggcgc ggtggccttc
     8041 acggcctcag acgtctcggt ggcccgggac cgtttcgtgc gcgaggactt cttcaaccga
     8101 gcctggggat tgccgctgta ctactgtgcc cagctgttgc tggcgttgag cccggccgcc
     8161 gtggcgagct gatgggcacc tgcctgcaat cgagcgagca gctgcccgaa gcaccacgcg
     8221 cgcccgcgcc gtggtcgctg cgtggccacg gctggatcgt gctgctgcgg ctgccgcgca
     8281 ccgcaccggc gcgaacggcc ttcgtgcccg cggcgctgca agcctcgctg cgtgcggcga
     8341 tctcggcgct cgtctgcgtc gactacgtca gcgcgccgtg cggcgcatat cgagagctgc
     8401 tgctgatccc gggcacgatg cgattcccgg acggccgacg ctacgcctcg atcagccgca
     8461 tcctggtgtc gacctgggcc agcgtcgcga acgggcgagc gaactggggc atcccgaagg
     8521 accgcgcgga ttcgttcgag atcgagcgtg gtgcggcctc ggaacggttc cgcgtcgccg
     8581 acggcgggcg cgagctctgt cgactggact tcgagccacc gcgcgggccg cgtctgccgc
     8641 tgcgcaccgc gtgggtaccc gcgagatggg ggacgctcgc gcagctgcac gggcagcgcg
     8701 cgtactacta ccggccggcc gcgcgcggat cgctgcgccc ggtgcgactg gcgcacgcgc
     8761 gctacgacgg cacgctgttc ccggacctct cggtggcgac ggtgcttgcc gcgttccgcg
     8821 tcgaggagtt cacgatggag ttcccggtcg cgcgcgtgga ggtcgtcgcg gcgacgtgag
     8881 gccgcgtagt agtggatgta cagcacgcgg cggaaaggct caccgcgttg ggaccggcac
     8941 ttcagacgtc tgcgcagcgg ccgtcccggc ccgctcagca cgacctgtga agacacgccg
     9001 taaacccgtc cctgggggct cgcgctcgcc atccataact cgcgacggtc ttcacaggtc
     9061 gtcctgaacg gaccaggccg gccgcagcag tgcgacgaga tcgcgcgcgg atggggggcg
     9121 aggcgggtcg ccggtcacga cccgcctcgc aggacgtccc tgtcgttgca ggaacgtcag
     9181 ccgaccttcg ctgcttcctt acggacggcg tgcaccg
//
LOCUS       DASPFR010000394         2946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_849637, whole genome shotgun sequence.
ACCESSION   DASPFR010000394 DASPFR010000000
VERSION     DASPFR010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849637"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /locus_tag="VD737_10480"
     CDS             complement(<1..1535)
                     /locus_tag="VD737_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXW11035.1"
                     /translation="MLYRFGDFELDLDRIELRTQGTGERIPLEPQVFALLALLVDARD
                     RMVSKGEIFEKVWDGRIVTDSALASRIKSARAALGDDGRTQRYIRTVHGRGFQFVADV
                     RIAASPASTPLADTAAPPLPTDGPGAKPPSIAVLPFWLTGSASDPYTVTLADALPHEL
                     IVDLSRLRWLLVTARASSFRLRDQDLDAGEVGRLLGVRYCLSGSIEQVRGARLHVSVE
                     LVDTRDGRVVWAERYDVPGDDVHQVRTDIRARILAALELRIPLHEAALARLGATESLD
                     AWSAFHLGLQHVYRFNRADTAQARALFELAVQRDPRFARAHAGLSFVHFQSAFLRQTD
                     DPAGATAQARGCAERALALDPLDPFVNFTMGRTYWLTGELDTALGWLERSTTLSPNFA
                     QGVYARGWTETLAGRGVEGREHVDHAMRLSPLDPLHYAMLGTRALGHMAQAEDETAAQ
                     WADRAARAPGAHVLIAMIAAASHSLAGHPDRARDWATNVRERNPVLTHDDFFRAFPMR
                     AAGP"
     gene            complement(1591..2613)
                     /locus_tag="VD737_10485"
     CDS             complement(1591..2613)
                     /locus_tag="VD737_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11036.1"
                     /translation="MSEARQTLRPGSTADPFGEHPARLQRRRVHLLGASFDFESPSAA
                     LLRLVDDAYAELPRHRLPGVTPRFRIRLQFTSGGRGTATTEPPAPHYHGGAGLLAAVM
                     DADNFAALCPDERSALVAISRSALRHSPRLVRYELLEFAVFALAHRGLRLVSLHAACL
                     GHARRGVLLIGESGAGKSVLSLHGALRGLDFLTEDSTFVDTRAMQATGISSFLHLRPD
                     ALRWLDDDALGRRIRRSPTIRRRSGVEKLEVDMRRIRPPARAPVTLAGIVLVSGRPAG
                     KGAPLAPVGRRALLDRLESSQAYAAQQAGWSAFVKKAATLPAFELRRASHPAAGAEAL
                     RDLLGR"
     gene            2702..>2946
                     /locus_tag="VD737_10490"
     CDS             2702..>2946
                     /locus_tag="VD737_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11037.1"
                     /translation="MLSVLVAHSYFLEFDQKQRDRAKPYPPLATLQVAAMLRDMGHRV
                     SLFDAMLASGPAQYSDRLGAVGPQVALFYEDNYNFLT"
BASE COUNT          446 a         1044 c         1067 g          389 t
ORIGIN      
        1 ggtcccgcag cacgcatcgg gaaagcgcgg aagaagtcgt cgtgcgtcag caccgggttg
       61 cgctcgcgca cgttggtcgc ccagtcgcgc gcgcgatcgg ggtgaccggc gagcgaatgg
      121 ctggcggcgg cgatcatcgc gatcagcacg tgggcgccgg gcgctcgcgc ggcgcggtcg
      181 gcccattgcg ccgccgtctc gtcctccgcc tgcgccatgt ggcccagcgc ccgcgtgccg
      241 agcatcgcgt agtgcagtgg atcgagcggg ctcagccgca tcgcgtggtc gacgtgttcg
      301 cggccctcga cgccgcggcc ggccagcgtc tccgtccagc cccgggcgta cacgccctgc
      361 gcgaaattgg ggctcagcgt ggtcgaccgc tcgagccagc cgagcgccgt gtcgagctcg
      421 ccggtgagcc agtacgtgcg tcccatcgtg aagttgacga acgggtcgag cgggtcgagc
      481 gcgagcgcac gctccgcgca gccgcgcgcc tgtgccgtgg cgcccgcggg atcgtcggtc
      541 tggcgcagga atgcggactg gaaatgcacg aacgacaggc ccgcgtgcgc gcgcgcgaag
      601 cgcgggtcgc gctgcaccgc cagctcgaac agcgccctgg cctgggccgt gtcggcgcgg
      661 ttgaagcgat acacgtgctg cagccccagg tggaacgccg accacgcgtc caggctttcc
      721 gtcgcgccga ggcgcgcgag cgcggcctcg tgcagcggaa tgcgcaactc gagcgcggcc
      781 aggatgcgcg cgcggatgtc cgtgcggacc tggtgcacgt cgtcgccggg cacgtcgtag
      841 cgctcggccc agaccactcg gccgtcgcgc gtgtcgacca gctccactga aacgtgcagg
      901 cgtgcgccgc gcacctgctc gatcgagccc gacaggcagt agcgcacgcc gagcaggcgc
      961 ccgacctcgc cggcgtcgag gtcctggtca cgcagccgga acgacgaggc ccgcgccgtc
     1021 accaacagcc agcgcaggcg cgacaggtcg acgatcagct cgtgcggcag cgcgtcggcg
     1081 agcgtcacgg tgtacggatc actggccgag cccgtcagcc agaacggcag caccgcgatg
     1141 gacggaggct tcgctccggg gccgtccgtc ggcagcggcg gcgcagccgt gtcagccagc
     1201 ggggtcgagg ccggcgaggc cgcgattctt acgtccgcga cgaactggaa accgcggccg
     1261 tgcacggtcc ggatgtagcg ctgcgttcga ccgtcgtcgc cgagcgcagc ccgagccgac
     1321 ttgatgcggc tcgcgagtgc cgagtcggtg acgatccgcc cgtcccagac cttctcgaag
     1381 atctcgccct tgctgaccat gcgatcgcgc gcgtcgacca gcagcgcgag cagcgcgaac
     1441 acctgcggct cgagcgggat gcgctcgccg gtgccctgcg ttcgaagctc gatccggtcg
     1501 aggtcgagtt cgaagtcgcc gaaccgatac agcatgccgt caggatacct gctgccgcgc
     1561 gcaaaacggc gacccgggtg cgggggcgcg ctatcgaccc agcaggtcgc gcagcgcttc
     1621 ggcgccggcg gcggggtgcg acgcgcggcg cagttcgaag gcgggcaggg tggcggcctt
     1681 cttcacgaac gccgaccagc cagcctgctg cgcggcatag gcctggctcg attccaggcg
     1741 atcgagcaac gcccgccgcc ccacgggagc caacggcgca cccttgccgg ccggccggcc
     1801 ggacaccagc acgatgccgg cgagcgtcac cggcgcgcgg gcaggcggcc ggatgcgccg
     1861 catgtccacc tcgagcttct ccaccccgct gcggcgacgg atggtgggcg agcgccggat
     1921 gcgccgaccc agggcgtcat cgtccagcca gcgcagcgcg tccgggcgca ggtgcaggaa
     1981 actcgaaatt ccggtcgcct gcatcgcccg cgtgtcgacg aacgtcgaat cctcggtcag
     2041 gaagtcgagg ccgcggagcg ccccgtgcag ggacagcacg gacttgccgg cgccgctctc
     2101 gccgatcagc agcacgccac ggcgggcgtg gccgaggcag gccgcatgca gggagacgag
     2161 ccgcaggccg cggtgcgcca gcgcgaagac ggcgaattcg agcagctcgt accgcacgag
     2221 gcggggcgag tgtcgcagcg cggaccgcga tatcgcgacg agtgcactgc gttcgtcggg
     2281 gcagagcgcg gcgaaattgt cggcgtccat cacggccgcc agcaggccgg caccaccgtg
     2341 gtagtgcggc gcgggcggct ccgtcgtcgc ggtcccgcgg ccgccgctcg tgaactgcag
     2401 ccggatccgg aaccgcggcg tcacgcccgg caggcgatgg cggggcagtt ccgcgtaggc
     2461 gtcgtcgacc aggcgcagca acgcggcact gggactctcg aagtcgaacg acgcgccgag
     2521 caggtgcacg cggcggcgct gcagccgggc cgggtgctcc ccgaacggat cggccgtgga
     2581 acccggtcgc aacgtttgtc ttgcctcact catcgctgaa ccgattcgcg gattggcggc
     2641 acctactgca ttgccagtcg actgcccggc gacgacatac taatcggaag cccctgccat
     2701 catgctgtcc gtcctggttg cccattcgta cttcctcgag ttcgaccaga agcagcggga
     2761 ccgcgccaag ccatatccgc cgctcgcgac gctgcaggtc gcggccatgc tgcgcgacat
     2821 ggggcaccgc gtatcgctgt tcgacgcgat gctggcgagc ggcccggcgc agtattccga
     2881 ccggctgggg gccgtcgggc cacaggtcgc gctgttctac gaggacaact acaacttcct
     2941 gaccaa
//
LOCUS       DASPFR010000395         9830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_118315, whole genome shotgun sequence.
ACCESSION   DASPFR010000395 DASPFR010000000
VERSION     DASPFR010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9830
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118315"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VD737_10495"
     CDS             <1..407
                     /locus_tag="VD737_10495"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11038.1"
                     /translation="GLSLVDRVAGLRLNEVAPSSLQGLALGLDALPSTDRAKVEVLPG
                     PAGVATRALDGADVVIVDPPRKGLEPAVLQALCERGPRRLVYVSCGFDTFLRDAAQLV
                     TEGGYAATALRAYDLLPYTDHVETVACFERRG"
     gene            714..947
                     /locus_tag="VD737_10500"
     CDS             714..947
                     /locus_tag="VD737_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11039.1"
                     /translation="MADERDSERSGQQQQRQDRGFEQPGGQQGLPHQDDERPDRDQTQ
                     QPRPTWGEQTPELDRQRSEGGGGSSSDSDGQRQ"
     gene            1097..2788
                     /locus_tag="VD737_10505"
     CDS             1097..2788
                     /locus_tag="VD737_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11040.1"
                     /translation="MSRFLVAMVVALALAAGLYVGNPARSNVSQEAPAVAAAPVGATL
                     IPGLGNYAFAITTSKPEAQRWFNQALMLTFGFNHDAAERSYLKAAEIDPDCAMCWWGA
                     SLVLGPHVNAAMDPADNPKAWQRLQRAMALAPKASVRERAYIKALEARYAREAPEDRR
                     SLDEAYAKATGELVRERPEDLDAKVFHAEALMDLQPWDYYDEDLKPKGGTAKVVSLLE
                     EVMKKNPDHAGALHLYVHAVEASADPHRGVVAADKLRTLIPGSGHLVHMPAHIYARVG
                     RWNDAVVANQVAIKADNEYLAMCRGNYKGVYPLGYVPHNPHFLWFAASMSGESKIAQD
                     AARMTAERVNLPELMREPGFAGLQHYWLTPWFDDVRFGRWDAIRERANPAADLPYVTA
                     IWHYAQGMAAVRTNRLDDARKHHEALAKLSADPLMDTLLVWDRYPLAHSARIAERVVA
                     GEIALAQGDEDVAIRALEDAVKIEGKIPYDEPPGWHAPVRQTLGAVLLQAGRAADAEQ
                     VYREELRRNPVNGWSLKGLALSLEAQGKREDAARVAKDYATAWQHADVQLVASRF"
     gene            complement(2862..4043)
                     /locus_tag="VD737_10510"
     CDS             complement(2862..4043)
                     /locus_tag="VD737_10510"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HXW11041.1"
                     /translation="MRSGAGFTHCLAMATFGAMLAGGASAADPPRARDLGIPFPGRPG
                     PLNAITDVSGVEVGYTTLIAGEGALQVGKGPVRTGVTAVLPRGRRNVSGVFGGYAVGN
                     GNGEMTGTIWLEESGILGTPICITNTHSVGVVRDAVIDWMVRNGGTEQLWGLPVVAET
                     WDGYLNDLNGFHVKPQHVFAALDGARGGAIAEGNVGGGTGMHTFEFKGGTGTSSRLVR
                     IRDVGDYTVGVLVQSNFGRRSQLQVAGIPVGREWPEDAPYTQEYGPVNELGSIIVVVA
                     TDAPLLPTQLKRIAARSAMGIARTGGTAGNGSGDIFIAFSTANAPAANADRGLQQVSF
                     VPNDQLDGLFEATVLAVEESIVNAMVAARTMTGVDGNTLVAIDHERLRGILARYNRIE
                     R"
     gene            4333..5976
                     /locus_tag="VD737_10515"
     CDS             4333..5976
                     /locus_tag="VD737_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HXW11042.1"
                     /translation="MHCNRVAVAAATALGLACVATTTTEAATDDVALVSVNASGSQAA
                     GDSNLRHGGGALSADGRYVVFHSYRASLVPNDRNRVADVFLRDLDHGTTRLVSGAWNG
                     GSANSYAVDASISADGRHAAFYSPASNLVQDDSNGAGDIFVWSREAGTLEIVSVNSAG
                     RPGNDESLESRISADGRYVAFTSFASNLARNDDNGRSDVFLRDRQAGYTERVSVASDG
                     REAQGYSWQPSVSANGRYVAFYSTAANLVPGDRGSAADVFVRDRRTRQTRRASEAADG
                     TPANGYSVQPVLSANGRYVAFVSDARNLVPGDTNRVIDVFVKDLETDAVERVSVASGG
                     AQADGASFEPSISADGRYVAFESFASNLVAGDTNGRNDVFVHDRTTHTTRRVSLTADG
                     RAANDDNWTPSISADGRFVAFRSDASNLAAIDGNRSSDVYVKDLGPRGTGGSSFTLRP
                     SALDFGSRDVNTATTQSFWLRNRAPTFLAIEGMEVRGTGGTAAYAVTHACGALLAPGD
                     VCGIHVTFTPTAVGPATAELKVRAEGGVTRTRELIGVGR"
     gene            6116..7951
                     /locus_tag="VD737_10520"
     CDS             6116..7951
                     /locus_tag="VD737_10520"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HXW11043.1"
                     /translation="MRKLGKVLAWTFGALAAALVAASMWFWFTPVGLDNYVNKITLQL
                     TLDSPQILSQLGFIDNTALDFHSGKLDDYTRTHEQDVLERLRRARAGLDRYGPDGLEG
                     QQLTTWNVTAWFLDDLIREAEFEHPGYRVNQISGPTVEVPQFLTDTHVIRDARSVERY
                     LSRLAEFGRVLREVHARVEQDRDAGVVPPDFVIDQALTGMRAFIAGGAAQNALVTTLP
                     ARLEKVEDLDDAQRDDYVRRAEAVVAREVIPGYEAMIALFEAMRPTATHDAGIWRIPG
                     GEQYYAAQLMSNTTTRMSADEIHQLGLAEVARIEGEMTAILDAQGVPGASLAERIRTV
                     SADPQQTFANDDTGRAQMLAFLKDVDRRVMAVAPQYFKTIPPQPLEIVRVPEYSQDSA
                     PGGYYHPPALDGSRPGRFYINQKDTKDNPRWTLPTFMIHEGSPGHHFQLSAQQLIEDV
                     PLLRKVLPFSAYSEGWALYVERVAKTDMGLYDSDPFGDLGRLQAEMFRAVRLVVDTGM
                     HARQWSREQSIDYMVSKTGMTEAEVTREIERYVVWPGQATSYKVGQLALLRMREDAER
                     ALGPKFDLRDFHEVVLMNGAMPLAVLEGIVGRWVEAQLQDASAAT"
     gene            complement(7978..8187)
                     /locus_tag="VD737_10525"
     CDS             complement(7978..8187)
                     /locus_tag="VD737_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11044.1"
                     /translation="MIVICPSCRAPSNTTEAHCWVCRREFDGSEPVIGDLPDARRPYR
                     LADVLEFEARRDGFDRVPWRHLRAS"
     gene            complement(8364..8786)
                     /locus_tag="VD737_10530"
     CDS             complement(8364..8786)
                     /locus_tag="VD737_10530"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXW11045.1"
                     /translation="MTYQASCACGAVEVELSGEPAVQAYCHCESCRRWLSAPVHAATL
                     WPTPNVRVTTGSDNLGMYKRTESSHRQFCRSCGTAVLIKHPALGLTDLPSAVVKGLSF
                     QPAVHVNYGEKVLSVRDGLPKFKDFPKDFGGSGDTLPE"
     gene            complement(8839..9513)
                     /locus_tag="VD737_10535"
     CDS             complement(8839..9513)
                     /locus_tag="VD737_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11046.1"
                     /translation="MPASTLEPMARLTLAALLTLAGLGGCVQAPTVHGEWQAGAKRGE
                     PYSRVLVVGVSHNLKNRCAFERYLAGQLQSDATEAWASCDSIDKDAREPLTRESIEAA
                     VAARKADAVIATVLVAQDMQAVQGGGRDTRGTAGYKATGAGWWDSYYGAYGVPVVYGE
                     FKSTSEITTLQADVTVASRVFETKGARVVYALETRARRLESRAEGLSSLTGPIAEQLR
                     RDGLIR"
BASE COUNT         1554 a         3420 c         3436 g         1420 t
ORIGIN      
        1 tcggcctttc gctggtcgac cgcgtcgcgg gcctgcggct gaatgaagtc gcgccgtcct
       61 cgctgcaggg actcgcgctc ggcctcgacg ccctcccatc gaccgaccgc gccaaggtcg
      121 aggtgctgcc cgggccggcg ggcgtggcga cccgcgcgct cgacggcgcc gacgtcgtga
      181 tcgtcgaccc cccgcgcaag ggcctcgagc cggccgtgct ccaggcactc tgcgagcgtg
      241 gcccgcgccg gctggtctac gtgagttgcg gcttcgatac attcctgcgc gatgccgcgc
      301 agctcgtgac cgagggcggc tatgcggcga ccgcgctgcg cgcctacgac ctgctgccct
      361 acaccgatca cgtcgagacg gtcgcctgct tcgagcgccg gggctgataa cgccctgcgg
      421 ggtggacaga gatgcgcgag ttcgcgcgcg gcgtgctcgc ccacttcaag gtgccgaccg
      481 cgctgcgcgg cggccggggg gccccgcgac tcggccgcga cgccgcgtgc atggagtcag
      541 gaaagacctg ctcgcaggtg cggggcagcg agccgcaact ccgtgcgcat gctccggtca
      601 ctccaatcga ggcggagagg cgccaagccg atagcgggcg aaaggtgccg gtgttacggt
      661 cttaggaaca tagggaatcg tgacaccggc acccacccca aggagggcac gacatggcgg
      721 acgaacgcga cagcgaacgg agcggccagc agcagcaacg ccaggaccgc ggcttcgagc
      781 aaccgggtgg gcagcagggc ctgccgcacc aggacgacga gcgtcccgac cgtgaccaga
      841 cgcagcagcc gcggcccacg tggggcgaac agacgcccga actcgaccgc cagcgctcgg
      901 aaggcggcgg cggttccagc agcgacagcg acggccagcg tcagtgagct gacgctcgag
      961 gccggccggt tcgcggtgcc gcccgggcat ttccgcactc ggcctacaga caaacaccca
     1021 acaaaagctt agaaggcgac cttcaccggt cgcccaagtc gtctctccac gcatgaccgg
     1081 tgggaggggg gtcgcaatga gtcgatttct ggtcgcgatg gtcgtagcgc tggctctcgc
     1141 cgcggggctt tatgtcggca acccggcacg ttccaacgtc tcgcaggaag ccccggccgt
     1201 ggcggccgcg ccggtcggcg cgacgctgat cccgggcctc ggcaactacg cgttcgcgat
     1261 cacgaccagc aagcccgagg cgcagcgctg gttcaaccag gcgctgatgc tcaccttcgg
     1321 gttcaaccac gacgccgccg agcgctcgta cctgaaggcg gccgagatcg acccggactg
     1381 cgcgatgtgc tggtggggcg catcgctggt gctggggccg cacgtcaacg cggccatgga
     1441 ccccgccgac aaccccaagg cgtggcagcg gctgcaacgg gccatggcac ttgcgccgaa
     1501 ggcctcggtg cgcgaacgcg cgtacatcaa ggcgctcgag gcgcggtacg cgcgcgaagc
     1561 gccggaggac cgccgcagcc tcgacgaggc ctatgccaag gccaccggcg aactcgtgcg
     1621 cgaacgtccc gaggacctcg acgcgaaggt gttccacgcc gaggcgctca tggacctgca
     1681 gccctgggac tactacgacg aggatctcaa gcccaagggt gggacggcaa aggtggtgtc
     1741 gctgctcgag gaggtgatga agaagaaccc cgaccacgcc ggcgcgctgc acctctacgt
     1801 gcacgccgtg gaggcctcgg ccgacccgca tcgcggcgtg gtcgccgccg acaagctgcg
     1861 cacgctgatc cccggctcgg gccacctcgt gcacatgccg gcgcacatct acgcgcgcgt
     1921 cggtcgctgg aacgacgccg tcgtcgcgaa ccaggtcgcg atcaaggcgg acaacgagta
     1981 cctcgcgatg tgtcgcggca actacaaggg cgtctatccg ctcgggtacg tgccgcacaa
     2041 cccgcacttc ctgtggttcg cggcaagcat gagcggcgag agcaagatcg cgcaggacgc
     2101 agcgcgcatg acggccgagc gggtgaacct gccggaactg atgcgcgagc ccggcttcgc
     2161 ggggctgcag cactactggc tgacgccgtg gttcgacgac gtgcgcttcg gccgctggga
     2221 cgcgatccgt gagcgtgcga atccggcagc ggacctgccg tacgtcaccg cgatctggca
     2281 ctacgcgcag ggcatggcgg ccgtgcgcac caatcggctc gacgacgcgc gcaagcacca
     2341 tgaagcgctc gcgaaactct cggccgatcc tctcatggac acgctgctgg tctgggaccg
     2401 ctacccgctc gcgcactcgg cgcgcatcgc cgaacgcgtc gtggcgggcg agatcgcgct
     2461 ggcgcagggc gacgaagacg tcgcgatccg cgcgctcgag gatgccgtga agatcgaagg
     2521 caagattccg tacgacgagc cccccggctg gcatgcaccg gtgcggcaga cgctcggggc
     2581 cgtgctgctg caggcgggcc gcgccgcgga tgctgagcag gtctatcgcg aggaactgcg
     2641 tcgcaatccg gtcaatggct ggtcgctgaa ggggctcgcg ctctcgctcg aggcccaggg
     2701 caagcgcgag gacgccgccc gcgtggccaa ggactacgcc acggcgtggc agcacgccga
     2761 cgtgcaactg gtcgcgtcca ggttctaatc gtctcgtaca cgaccactga gccggccgtc
     2821 acccgggctc ctctcccgcg agcgcgggag aggtgcccga ctcaccgctc gatccggttg
     2881 tagcgcgcga ggatgccacg caggcgctcg tggtcgatcg cgacgagtgt gttgccgtcg
     2941 acgccggtca tggtgcgcgc cgccaccatc gcgttgacga tcgactcctc gacggccagc
     3001 accgtcgcct cgaacaggcc gtcgagctgg tcgttcggca cgaagctcac ctgctgcagc
     3061 ccgcggtccg cgttggcggc gggcgcgttg gccgtcgaga acgcgatgaa gatgtcgccg
     3121 gagccgttgc cggccgtgcc gccggtgcgc gcgatgccca tcgcggaccg tgccgcgatg
     3181 cgcttgagtt gcgtgggcag caggggcgcg tcggtcgcga cgacgacgat gatcgagccg
     3241 agttcgttga ccgggccgta ttcctgcgtg taaggcgcgt cctccggcca ttcgcggccg
     3301 accggaatgc cggcgacctg cagttgcgag cgtcgcccga aattcgactg cacgagcacg
     3361 ccgacggtgt agtcgccgac gtcgcggatt cgcacgagtc gcgacgacgt gccggtgccg
     3421 cccttgaact cgaacgtgtg catgcccgtg ccgccgccca cgttgccctc cgcgatcgcg
     3481 ccgccgcgcg cgccgtcgag cgccgcgaac acgtgctgcg gcttgacgtg gaagccgttg
     3541 aggtcgttca ggtaaccgtc ccaggtttcc gcgacgacag gcagtcccca cagctgctcc
     3601 gtgccgccgt tgcgaaccat ccagtcgatc acggcgtcgc gcaccacgcc gacactgtgg
     3661 gtgttggtga tgcagatcgg cgtgccgagg atcccggact cctcgagcca gatcgttccg
     3721 gtcatctcgc cgttgccgtt gccgaccgcg tagccgccga agaccccgct cacgttcctg
     3781 cggccgcgcg gcagcaccgc ggtcacgccc gtgcgcacgg gccccttgcc gacctgcagc
     3841 gcgccttcgc ccgcgatgag cgtcgtgtag ccgacctcca cgccggacac gtcggtgatg
     3901 gcgttcaagg gaccgggtcg gccggggaac gggataccga gatcgcgggc gcggggcgga
     3961 tcggcggccg acgccccgcc cgccagcatt gcaccgaagg tggccatcgc gaggcaatgc
     4021 gtgaaccctg cgcccgatct catgactcac ctccgccgct ggggagcgtg atgatcacag
     4081 ggacaccatg gtcgcgtcca acggttcgct gcgtcgtcgg gaagcgtttt ccgtcggccg
     4141 acctcgcaag aagccgccgc aggcgaagcg agccgacctc gagccgtcgg accttcacgc
     4201 gaaagcttgt cagtccttgc gctgcctccc cggcaggatg cgcgcaactt cggaggctct
     4261 gactgactgc gcgccgcggc gcgcgagact gcgcgcaagg cttcaaatat cgattaattg
     4321 ggggaatcag caatgcactg caaccgggtc gccgtcgccg ccgcgaccgc gctgggactg
     4381 gcctgcgtcg cgacgacgac caccgaagcg gccaccgacg acgtggcgct ggtgagcgtc
     4441 aacgcctctg gaagccaggc cgctggcgac agcaatctgc gacatggcgg gggcgccttg
     4501 agcgcggacg gccgctacgt cgtcttccac tcgtaccgcg cgagcctcgt gccgaacgat
     4561 cgcaatcgcg tcgccgacgt gttcctgcgc gacctcgacc acggcaccac gcgcctcgtg
     4621 agtggcgcct ggaatggcgg cagcgccaat tcgtacgccg tcgatgcgag catcagcgcg
     4681 gacggccgcc acgcggcctt ctactcgccg gcgagcaacc tcgtgcagga cgacagcaac
     4741 ggcgccggcg acatcttcgt ctggagccgc gaggctggca cgctcgagat cgtgagcgtc
     4801 aactcggcgg gccgccccgg caacgacgag agcctggagt cgaggatcag cgccgacggg
     4861 cgctacgtcg cgttcacgtc gttcgcgagc aacctggccc gcaacgacga caatgggcgt
     4921 tcggacgtgt tcctgcgcga ccggcaggcc ggttacaccg agcgtgtcag cgtggcctcc
     4981 gacgggcgcg aagcgcaggg ctacagctgg cagcccagcg tcagcgcgaa cgggcgctac
     5041 gtcgccttct actcgaccgc cgccaacctc gtgccgggcg accggggcag cgcggccgac
     5101 gtgttcgtgc gcgaccgacg gacgcggcag acgcgacggg cgagcgaggc cgcggacggt
     5161 acgcccgcga acggctacag cgtgcagccc gtgctgagtg ccaacggacg ctacgtggcg
     5221 ttcgtctcgg acgcccgcaa cctggtgccg ggcgacacca accgagtgat cgacgtcttc
     5281 gtgaaggatc tcgagacgga cgccgtcgag cgcgtgagcg tcgcgtcggg cggcgcgcag
     5341 gccgacgggg ccagcttcga accttcgatc agcgcggacg ggcgctacgt cgccttcgaa
     5401 tcgttcgcat cgaacctcgt cgccggcgac accaacggcc gcaacgacgt gttcgtgcac
     5461 gaccggacga cacacacgac gcgccgcgtg agcctgacgg ccgacggccg tgcggccaac
     5521 gacgacaact ggacgccctc gatcagcgcg gacgggcgtt tcgtcgcgtt ccgttccgac
     5581 gcgagcaatc tcgccgcgat cgacggcaac cgctcgtccg acgtctacgt gaaggacctc
     5641 gggccgcgcg ggacgggcgg gtcttcgttc acgttgcggc cgtcggcgct cgatttcggc
     5701 tcgcgcgacg tcaacacggc cacgacgcag agcttctggc ttcgcaaccg ggccccgacc
     5761 ttcctcgcca tcgagggtat ggaggttcgg ggtaccggtg gcaccgctgc gtatgcagtc
     5821 acgcacgcct gtggcgcgct cctggcgccg ggcgacgtct gcggcatcca cgtgacgttc
     5881 acgccgaccg cagtcgggcc ggccacggcc gaactcaagg tgcgtgcgga aggaggcgtg
     5941 acgcgcaccc gggagctgat cggcgtgggt cggtagggtc ccgtccacga tccaacccgt
     6001 cgccgccgtc gtgcacgggc gctcgcgctg acgcggcgcc cggccgcagg cgctgcggcc
     6061 tccctcgcag gcttggggta tagtcgcgcg ctttccgcct cgggagggag gggcgatgcg
     6121 caagctgggc aaggtgctgg cctggacgtt cggcgcgctg gctgcagcgc tggtcgcggc
     6181 gtcgatgtgg ttctggttca cgccggtcgg cctggacaac tacgtcaaca agatcacgct
     6241 gcagctgacg ctcgattcgc cgcagatcct gtcccagctc ggcttcatcg acaacacggc
     6301 gctcgacttc cacagcggca agctcgacga ctacacccgc acgcacgagc aggatgtgct
     6361 cgagcggctg cgccgcgcgc gcgccggact cgaccggtat ggacccgacg gcctcgaagg
     6421 gcagcagctc accacctgga acgtcaccgc ctggttcctc gacgatctga tccgggaggc
     6481 cgagttcgag caccccggct atcgggtcaa ccagatttcg ggaccaacgg tggaggtgcc
     6541 gcagttcctc accgacacgc acgtgatcag ggacgcgcgc agcgtcgagc ggtatctctc
     6601 gcgcctcgcc gagttcggcc gggtcctgcg cgaggtgcac gcgcgcgtcg agcaggaccg
     6661 cgatgccggc gtcgtgcctc ccgacttcgt gatcgaccag gcgctgacgg gcatgcgtgc
     6721 cttcatcgcc ggaggcgccg cgcagaacgc gctcgtgacc acgctgccgg caaggctcga
     6781 gaaggtcgag gacctcgacg acgcgcagcg tgacgactac gtccggcgtg ccgaggccgt
     6841 cgtcgcgcgc gaagtgattc cgggatacga ggcgatgatc gcgctgttcg aggccatgcg
     6901 cccgaccgcg acgcacgacg cggggatctg gcgcatccca ggcggcgagc agtattacgc
     6961 ggcgcagctc atgtcgaaca cgacgacacg gatgagcgcc gacgagatcc accagctcgg
     7021 cttggccgag gtggcacgca tcgagggcga gatgactgcg atcctggacg cgcaaggcgt
     7081 gccgggcgcg agcctcgccg agcgcatccg tacggtgtcg gcagacccgc agcagacctt
     7141 tgccaacgac gacaccggcc gagcgcagat gctggccttc ctgaaggacg tcgaccgccg
     7201 cgtcatggcc gtggcgccgc agtatttcaa gacgatccct ccgcagccgc tcgagatcgt
     7261 gcgcgtgccg gagtattcgc aggattccgc gccgggcggt tactaccacc cgccggcgct
     7321 cgacggctcg cggccgggcc gtttctacat caaccagaag gacacgaagg acaacccgcg
     7381 ctggacgctg ccgacgttca tgatccacga gggctcgccg gggcatcact tccagctttc
     7441 ggcgcagcag ctgatcgagg acgtgccgct gctgcgcaaa gtgctgccgt tcagcgccta
     7501 ctcggaaggc tgggcgctct acgtcgagcg cgtcgcgaag accgacatgg gcctctatga
     7561 ctcggacccg ttcggcgacc tcgggcgact gcaggccgag atgttccgcg ccgtgcgact
     7621 ggtggtcgac acgggcatgc acgccaggca gtggtcgcgc gagcagtcga tcgactacat
     7681 ggtgtcgaag accggcatga ccgaggcgga ggtcacgcgc gagatcgagc gctacgtggt
     7741 gtggccgggg caggcgactt cgtacaaggt cggccagctc gccctgctgc ggatgcgcga
     7801 agacgccgag cgtgcgctcg gcccgaagtt cgacctccgc gacttccacg aggtcgtgct
     7861 gatgaacggc gcgatgccgc tggccgtgct cgagggcatc gtcggtcggt gggtcgaggc
     7921 gcagctgcag gacgcgtccg ccgccacctg aggcggcggg cggcgcgacg ccggaggtca
     7981 gctggcgcgg aggtggcgcc agggcacccg gtcgaatccg tcgcggcggg cctcgaattc
     8041 gaggacgtcg gccaggcggt acggccggcg ggcgtccgga aggtcaccga tgaccggttc
     8101 cgagccatcg aactcgcgac ggcagaccca gcagtgcgct tccgtcgtgt tgctgggcgc
     8161 gcggcacgat gggcagatca cgatcatggc agcaccgtcc ctggtgattg cacaggcgaa
     8221 ccgagcttaa aaaaactccc ttggccgcgt tgtagccgaa gcgctcacgt gcgcgcaggg
     8281 gggcgctcac acggcccgta agatcgggcg gccggcttcg ccgcgccgac ggcacccgcc
     8341 agtgcgcaga cgcgccgcgc ccgtcactcc ggcaacgtgt cgccggagcc gccgaagtcc
     8401 ttcgggaagt ccttgaactt gggcagcccg tcgcggaccg acagcacctt ctcgccgtag
     8461 ttgacgtgca cggcgggctg gaatgacagg cccttgacca cggccgaggg caggtccgtg
     8521 agccccagcg cgggatgctt gatcagcacg gccgtaccgc agctgcgaca gaactgtcga
     8581 tggctgctct cggtgcgctt gtacatgccg aggttgtccg atccggtcgt cacgcggacg
     8641 ttgggggtcg gccacaacgt cgccgcgtgc acgggcgcgc tcagccagcg tcggcacgac
     8701 tcgcagtggc agtacgcctg gacggcaggt tcgccgctca gctcgacttc gaccgcaccg
     8761 cacgcacaac tcgcctggta ggtcatggat cgtggctccc tgtttgcgac gaagcgtgaa
     8821 tggtacccaa gtccgcgttc aacggatcaa accgtcccgg cgcagctgct cggcgatcgg
     8881 gccggtgagg ctggagaggc cttcggcacg cgactcgagc cggcgcgcgc gggtctcgag
     8941 cgcatagacg acgcgggctc ccttcgtctc gaacacgcgc gacgccacgg tcacgtccgc
     9001 ctgcagcgtc gtgatttcgc tcgtgctctt gaactcgccg tagacgaccg gcacgccgta
     9061 ggctccgtag tagctgtccc accagccggc gcccgtggcc ttgtagccgg ccgtgccgcg
     9121 cgtgtcgcgt ccgccgccct ggaccgcctg catgtcctgc gcgaccagca cggtcgcgat
     9181 cacggcgtcc gccttgcgtg ctgccaccgc cgcttcgatg ctctcgcgcg tgagcggctc
     9241 gcgggcgtcc ttgtcgatcg agtcacagct ggcccaggcc tcggtcgcgt cgctctggag
     9301 ctgcccggcc aggtaacgct cgaaggcaca gcgattcttg aggttgtggg agacgcccac
     9361 gaccagcacc ctcgagtacg gttcaccgcg cttggcgccg gcctgccact cgccgtgcac
     9421 ggtcggcgcc tgtacgcagc cgccgaggcc ggcgagggtc agcagtgcag ccagggtcag
     9481 gcgggccatc ggctcgaggg tcgacgcagg catctgttcg ggctccacgg gatgatcgat
     9541 gcgcggcgcg cagggttcag ccttgtagca aaaaacgggg gcgcccgagc gaatccttgc
     9601 gacccggcgg ccgcaggggt gccccctcag cgcggcatcc gcgagcagcc gccccgcctg
     9661 cggcgcgcgg cgcgcggccc ccgcccaggc cgacgcggcg gccatggtca gtagctccgc
     9721 tcgagttcct cgcgcaccag cgcgagcagc ggctcgtagc cgaactcggg cagcggacgt
     9781 ccgttcacgc ggtactcggg cgtcgccgtg accttgagcg cgatggcgtc
//
LOCUS       DASPFR010000396         6553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_118737, whole genome shotgun sequence.
ACCESSION   DASPFR010000396 DASPFR010000000
VERSION     DASPFR010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118737"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1780)
                     /locus_tag="VD737_10540"
     CDS             complement(<1..1780)
                     /locus_tag="VD737_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXW11047.1"
                     /translation="MTLSYQTLPEMLDHRARGDGAIVYLEGDGAEKRLTLANLRRRAL
                     GILHHLQGTGAQPGDFLVLHVASNEQFIDAYWACLYGGIVPVPVAVGISDEHKHKLLR
                     IARRLGTPLLYTDRKLRDRLAAYASTAGESEVWNALEARTFLIDSLDDISRPGTPATI
                     TPDQTAFIQFSSGSTSEPKGVVLTHRNILSNAEGARQAAGFTEDDVSLSWMPLTHDMG
                     LIGFHLMMMYSGVRQHLMPTDLFVRRALLWLKWCSEKRVTLTCSPNFGYRHCLRALGN
                     KSLDGHDLSAVRLIFNGAEPISVELAAEFLDRLAPYALGRTAMYPVYGLAEASLAVCF
                     PPPGSAFKYVTVDRRQLGVGAQARLVDAGDPAALKLMCEGKPIPYTSVKLVDDAGDEV
                     AEGAVGHLLMRGDNVTRGYFDNAEANAAAISPDGWLRTGDLALMLDGEFYVTGRSKEI
                     IFVNGQNYYPHDLESILQGEPGLELGKVAAAGARAATEETDELVLFVLHRGNVADFLP
                     LATRAMHLVNEHAGVEVARVVPVKRMPKTTSGKLQRTALAQAYESGEFDSEVAEFDLA
                     WAQAHGHGRAAKGQIERQLKAIVDDAM"
     gene            1835..2482
                     /locus_tag="VD737_10545"
     CDS             1835..2482
                     /locus_tag="VD737_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11048.1"
                     /translation="MENASLLHAPLTPGLRRPCAARLLEALPYARRLHLERTDPLVRA
                     SSLAGLALALVAAARARGAAFEGIAGLRLPEGEKPRFVRGPHFSVSHTPTRVACIACD
                     AAEVGIDIETAPAGAASAVVRKLQRWTATEATLKAAGAGLRRVRDVRVELASLEAGFE
                     GRCYVLRELALAPDTLGHVASLQPVRPDIECVALDGSCFSASLERVLGLSTQDAQ"
     gene            complement(2432..3220)
                     /locus_tag="VD737_10550"
     CDS             complement(2432..3220)
                     /locus_tag="VD737_10550"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXW11049.1"
                     /translation="MNDARDDNKDEDERASAANEPLRNTLYRRLTKGQRRLTRGRMLM
                     YRVAVALGWSIIRTLWATCRVTHVSGLEAARQAVRDSKSLIPVYWHQHMLFGARAVLD
                     LHADGLKAGFLISPSIDGTAPAMLVEKVGGVVIRGSSTHTGARALRDYYETIVRQQVS
                     PAITPDGPKGPLHEFKPGAIMLAQITGKPILPIAVAAASAIRFRTWDAFELPLPFSRI
                     AIVYGEPVKVGRATDTAAFGQWQAKMAESLRVLRRQAEDALERR"
     gene            complement(3255..4145)
                     /locus_tag="VD737_10555"
     CDS             complement(3255..4145)
                     /locus_tag="VD737_10555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXW11050.1"
                     /translation="MKDRLALSHETQASSTSRLGWALAITTTFMVVEVVGGLLSGSLA
                     LLADAGHMLTDAAALALAWLAARLTMRPANARRSYGYHRAQVLAAFVNALALFGIVVW
                     ILLEAAQRLRTPQPIAGLTMLGVAFAGLCANVGVFLVLRDRSGDNLNVAAARLHVVGD
                     LLGSVGAIVAALIILATGWTPADPILSVLVAALILRSAWQLLGKSTRILMEGTPEGMD
                     PEELRRSLLVTLRGVRDVHDVHCWSLTTGQTMLTLHLALDPGTDPDGVLREAKRLLAD
                     RFDITHSALQVEHARCPDEV"
     gene            4289..5008
                     /locus_tag="VD737_10560"
     CDS             4289..5008
                     /locus_tag="VD737_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXW11051.1"
                     /translation="MTVVIPVCNEDENVLPLAREIHAALAGRYSFEQVFVDDGSTDRT
                     AEAVLAARAQGMPEIRLLRHVQRSGQSAAVATGVRHARGRWIATLDGDGQNDPADFPK
                     LLDALLDPGAPRRKLVMGNRVTRRDTWLRRLSSRVANGVRSSILRDGTPDTGCGIKVF
                     DRDVFMHMPRFRNMHRFMPALFQREGFEVISLPVNHRERTRGTSKYGLHNRLWVGIVD
                     LFGVSWLIRRASPRVQVHEET"
     gene            5005..>6553
                     /locus_tag="VD737_10565"
     CDS             5005..>6553
                     /locus_tag="VD737_10565"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXW11052.1"
                     /translation="MITAGGQSPSSQPLLERLLRPGAASRRDVVLDLLWLFGLALLMM
                     GAGFGLRDPWPADEPRFALIAQDMLRSGDWLVPRVGGDLYADKPPLYFWAMAASMALT
                     GSTRLGFLLPSLLSGLGVLGLVYDLLRRLRGRDVALAGAFVLLLTFQFVWQARQAQID
                     ATLLFLTTLSLYGLLRHLFAGPALGWFFVGWAAAGLGVITKGVGFLPLLALIPFAVLR
                     TRGWPAAVSARDPRWWLGPVFFLAAIGAWFVPMMLLTSAGGELLAYRNEILFQQTVTR
                     YAEAWHHHEPPWYYLTNVVPVLWLPLIALVPWLWPRWRRALSGAGAPDSRDTFVAVLL
                     AWVVIVLLFFSFSSGKRGVYVLPAVPALAMAAGPWLPELLLRRVGTQRLAFGLAAPLA
                     ITITLAAVYFAFADDATNRAVATYGLSPATPLAIAGVAGLGALVLFRVRDGWLAYAST
                     LAAVLVTVGFVVYPQIDAVRSGRAFMQRVADASRDLEELALVGSKEQYLLHLPRPSVN
                     FGHARWRE"
BASE COUNT          904 a         2251 c         2312 g         1086 t
ORIGIN      
        1 gcatcgcgtc gtccacgatc gccttgagct ggcgctcgat ctgccccttg gcggcgcgtc
       61 cgtggccgtg cgcctgggcc caggccaggt cgaactccgc gacctccgag tcgaactcgc
      121 cggactcgta ggcttgcgcg agcgcggtgc gctgcagctt gccgctggta gtcttgggca
      181 tccgctttac aggcacgacg cgcgccactt cgacgccggc atgctcgttg acgaggtgca
      241 tcgcgcgcgt cgcgagcggc aggaagtcgg cgacattgcc ccggtgcagc acgaacagca
      301 ccagctcatc ggtctcctcg gtcgcggcac gcgcgccggc ggcagcgacc ttgccgagct
      361 cgaggcccgg ttcgccctgc aggatcgact cgaggtcgtg cggatagtag ttctggccgt
      421 tgacgaagat gatctccttc gaccggcccg tgacgtagaa ctcgccgtcg agcatcagcg
      481 cgaggtcgcc ggtgcgcagc cacccgtcgg gcgagatcgc ggcggcgttc gcctcggcgt
      541 tgtcgaagta gccgcgagtc acgttgtcgc cgcgcatgag caggtgcccg accgcacctt
      601 ccgcgacttc gtcgccggca tcgtcgacga gcttgacgct cgtgtagggg atcggcttgc
      661 cctcgcacat caatttgagc gcggcgggat cgcccgcgtc cacgagtcga gcctgcgcgc
      721 cgacgccgag ctggcggcgg tccaccgtga cgtacttgaa ggcagatccc ggcggcggga
      781 aacagacggc gagcgacgct tcggcgagtc cgtagacggg gtacatcgcc gtcctgccga
      841 gcgcgtacgg cgccagacgg tcgagaaact ccgcggcgag ctcgaccgaa atcggctcgg
      901 ccccgttgaa gatcagccgt accgcgctga ggtcgtgtcc gtcgagggac ttgttcccca
      961 gcgcacgcag gcagtgtcgg tatccgaagt tcggcgagca ggtgagcgtg acgcgcttct
     1021 ccgagcacca cttgagccac agcagcgcgc gacgcacgaa caggtcggtc ggcatcaggt
     1081 gctggcgcac gcccgagtac atcatcatca ggtgaaaacc gatcagcccc atgtcgtgcg
     1141 tgagcggcat ccagctcagc gacacgtcgt cctcggtgaa cccggcggcc tgccgtgcgc
     1201 cctcggcgtt gctcaggatg ttgcggtgag tcagcaccac gcccttgggc tcgctggtcg
     1261 acccggagga aaactggatg aacgccgtct ggtcgggggt aatggtcgcc ggcgttccgg
     1321 gccgcgaaat gtcgtccagc gagtcgatca ggaaagtgcg cgcctccagg gcgttccaca
     1381 cctcggattc gccggccgtg gacgcgtatg cggcgagccg gtcgcgcagt ttgcggtcgg
     1441 tgtagagcag cggcgtgccc agccggcgcg cgatcctgag cagcttgtgc ttgtgctcgt
     1501 cgctgatgcc gaccgcgacc gggaccggca cgatgccgcc gtacaggcac gcccagtacg
     1561 cgtcgatgaa ctgctcgttg ctcgcgacgt gcagcaccag gaaatcgccg ggctgcgcgc
     1621 cggtgccttg caggtgatgc aggatgccga gcgcacgtcg acgcaagttg gcgagcgtga
     1681 ggcgcttctc cgcgccgtca ccctcgagat agacgatggc gccgtcgccg cgcgcgcggt
     1741 ggtcgagcat ctcgggcagg gtctggtaac tgagcgtcat cggtgtggtg acccgctggc
     1801 gtcgtccgca cgcgggcggc gacaatgcgt acgagtggaa aacgcaagct tactacatgc
     1861 cccgctcacg cccggactgc gccggccgtg cgccgcgcgc ctgctcgagg cgttgccgta
     1921 cgcgcgtcga ctgcacctcg agcgaaccga cccgctcgtg cgcgcgtcga gtctcgccgg
     1981 gctcgcgctc gcgctggtcg ccgccgcgcg ggcgcgcggg gcggcgttcg aagggatcgc
     2041 cgggttgcgg ttgccggagg gcgagaagcc gcggttcgtg cgcggtcctc acttcagcgt
     2101 ctcgcacacg ccgacgcgcg tcgcctgcat cgcatgcgac gccgccgagg tggggatcga
     2161 catcgagacc gcgccggcgg gagcggcgtc cgcggtggta cgcaagctgc agcggtggac
     2221 cgccaccgag gcgacgctga aggccgccgg cgccgggttg cgccgcgtgc gcgacgtgcg
     2281 ggtcgaactg gcgtcactcg aggccggatt cgaaggacgg tgttacgtgc tgcgtgaact
     2341 cgcgctcgcg cccgacacgc tcgggcacgt ggcatcgctg cagccggtac gtccggacat
     2401 cgagtgcgtg gcgctcgacg ggtcctgctt ctcagcgtcg ctcgagcgcg tcctcggcct
     2461 gtcgacgcag gacgcgcagt gactccgcca ttttcgcctg ccactgtccg aaggcggccg
     2521 tgtcggtggc ccggccgacc ttgaccggct cgccgtagac gatcgcgatg cggctgaacg
     2581 gcagcggcag ttcgaaggcg tcccaggtgc gaaaccggat cgcactggcg gcggccacgg
     2641 cgatcgggag gatcggcttg ccggtgatct gcgccagcat gatcgcgccc ggcttgaact
     2701 cgtgcagcgg gcccttgggc ccgtcgggcg tgatcgcggg cgacacctgc tgcctgacga
     2761 tggtctcgta gtagtcgcgc agcgcgcgcg cgccggtgtg cgtcgacgag ccgcgtatga
     2821 ccactccgcc cactttctcg acgagcatcg cgggggccgt gccgtcgatg gacgggctga
     2881 tcaggaaccc ggccttgagg ccgtcggcat gcaagtcgag cacggcgcgc gcgccgaaga
     2941 gcatgtgctg gtgccagtag acggggatca gcgacttcga gtcccgcacc gcctggcggg
     3001 cggcctcgag accgctcacg tgcgtcacgc ggcacgtcgc ccacagcgtc cggatgatgc
     3061 tccagccgag ggcgacggcc acccggtaca tcagcatccg cccgcgggtc agtcgccgct
     3121 ggcccttggt cagcctgcga tagagcgtgt tacgcaacgg ctcgttcgcg gcagatgcgc
     3181 gttcgtcttc gtccttgttg tcgtcccggg cgtcgttcat aggtcggttc gcggggtcgt
     3241 cgcaggtcgc tcgatcagac ttcgtccggg cacctggcgt gctcgacctg cagcgccgaa
     3301 tgcgtgatgt cgaagcggtc ggcgagcagt cgcttggcct cgcgaagcac gccgtccgga
     3361 tcggtgccgg ggtcgagcgc aaggtgcagc gtcagcatcg tctgcccggt ggtcaacgac
     3421 cagcagtgta cgtcgtgcac gtcgcgcacg cctcgaagcg tcacgagcag tgaccggcgc
     3481 agttcctcgg ggtccatgcc ttccggggtg ccctccatga ggatgcgcgt ggacttgccg
     3541 agcagctgcc aggcgctgcg caggatcagc gctgcgacca gcaccgacag gatcgggtcg
     3601 gccggcgtcc agccggtcgc caggatgatc agcgccgcca cgatggcgcc gaccgatccc
     3661 agcaggtcgc caaccacgtg gaggcgcgcg gccgcgacgt tcaggttgtc gccgctgcga
     3721 tcccgcagga ccaggaacac acccacgttg gcgcagagcc cggcgaaagc cacaccgagc
     3781 atggtgagcc ctgcgatcgg ctgcggcgtg cgcaggcgct gcgccgcttc gagcaggatc
     3841 cacaccacga tgccgaacag cgcgagcgcg ttgacgaatg ccgcgagcac ctgcgcacgg
     3901 tggtagccgt acgagcgacg cgcgttcgcc ggccgcatcg tcagcctcgc ggcgagccac
     3961 gccagcgcga gcgccgccgc gtccgtgagc atgtgccccg cgtccgcaag cagggcgagg
     4021 gagcccgaga gcaggccgcc gaccacttcc accaccatga acgtggtggt gatcgccagt
     4081 gcccatccga ggcgactcgt cgaggaggcc tgggtctcgt gcgaaagggc gagtcgatcc
     4141 ttcacgggcc gggagttccg cgcggcggcc gggctgccgc ccgagggagc gtttatacta
     4201 ccggacggcc ccgttcgcgg cggctgcaca aaccacgaac ggaaggcacc cggtgacccc
     4261 gccctcgaca cctgcgacgt cgcctgacgt gaccgtcgtc atacccgtct gcaacgagga
     4321 cgagaacgta ttgccgctcg cgcgcgagat ccacgcggcg ctcgcgggtc gctattcgtt
     4381 cgagcaggtc ttcgtcgatg acggcagcac cgaccgcacc gcggaagcgg tgctcgcggc
     4441 gcgcgctcag ggcatgccgg agatccggct gctgcgtcac gtgcagcgct cgggacagag
     4501 cgccgccgtc gcgaccggcg tgcgccacgc gcgcggtcgg tggatcgcga cgctcgacgg
     4561 cgacggccag aacgatccgg ccgactttcc gaagctgctc gacgcgctcc tcgatccggg
     4621 ggcgccccgg cgcaagctcg tgatgggcaa ccgagtgacg cgccgcgaca cctggttgcg
     4681 gcgcctgtcg tcgcgcgtcg ccaacggcgt gcgctcgtcg atcctcaggg acggcacgcc
     4741 cgacaccggc tgcggcatca aggtgttcga ccgtgacgtg ttcatgcaca tgccgcgctt
     4801 tcgcaacatg catcgcttca tgcccgcctt gttccagcgc gaaggattcg aagtgatctc
     4861 gttgcccgtc aatcaccgcg agcgcacccg cggcacgtcc aagtacggcc tgcacaaccg
     4921 gttgtgggtg ggcatcgtcg acctgttcgg cgtgagctgg ctgatccgtc gcgcgtcgcc
     4981 gcgcgtgcag gttcacgagg aaacatgatc accgcgggcg gccagtcgcc gtcgtcgcaa
     5041 ccgctgctcg aacgtctgct gcgcccgggg gccgcatcac gtcgtgatgt cgtgctcgac
     5101 ctgttgtggc tgttcgggct ggcgctgctg atgatggggg cggggttcgg gctgcgcgat
     5161 ccctggcctg ccgacgagcc gcgtttcgcg ctgatcgcac aggacatgct gcgctcgggc
     5221 gactggctgg tgccgcgcgt gggtggcgac ctctacgccg acaagccacc gttgtatttc
     5281 tgggcgatgg cggcctccat ggccctgacc gggtcgaccc ggctcggatt cctgctgccg
     5341 tcgctgctgt ccggcctcgg cgtgctgggg ctcgtctacg acctgctgcg ccgcctgcgt
     5401 ggtcgcgacg tcgcactggc cggcgccttc gtgctgctgc tgacgttcca gttcgtctgg
     5461 caggcccgac aggcgcagat cgatgcgacg ctgctgttcc tgaccacgct gagcctgtac
     5521 ggactgctgc gccacctgtt cgcaggcccg gcgctgggct ggttcttcgt cggttgggct
     5581 gccgcgggcc tgggcgtgat caccaagggc gtcggcttcc tgcccctgct ggcgctgata
     5641 ccgtttgccg tgctgcgcac gcgcggctgg cccgccgccg tctccgcgcg ggacccgcgc
     5701 tggtggctcg gcccggtctt cttcctggcg gcgatcggcg catggttcgt gccgatgatg
     5761 ctactcacct ccgccggcgg tgaactgctc gcctatcgca acgagatcct gttccagcag
     5821 accgtgacgc gctacgccga ggcgtggcac caccacgagc cgccctggta ctacctcacc
     5881 aacgtggtgc ctgtgctgtg gctcccgctc atcgcgctcg tgccgtggct gtggccgcgc
     5941 tggcggcggg cgttgtccgg agcgggtgcg ccggattcgc gcgacacgtt cgtggccgtg
     6001 ctgctcgcgt gggtcgtgat cgtgctgctg ttcttttcgt tcagttccgg caagcgcggc
     6061 gtctacgtgc tgcccgccgt gcccgccctg gcgatggccg ccggcccatg gctgccggaa
     6121 ctgctgctgc gcagggtcgg cacgcagcgc ctggcgttcg ggcttgcggc gccgctcgcg
     6181 atcaccatca cgctcgcagc ggtctacttc gctttcgccg acgacgccac gaaccgcgcc
     6241 gtcgcgacat acggcctgtc acccgccacg ccgttggcca tcgcaggagt ggccgggctg
     6301 ggcgcactcg tgctgttccg tgtgcgcgac ggatggctcg cgtacgcctc gacgctggcc
     6361 gccgtgctcg tcaccgtggg gttcgtcgtg tatccgcaga tcgacgccgt gcgttcgggg
     6421 cgggcgttca tgcagcgcgt ggcggatgcg tcgcgcgacc tcgaggaact cgccctcgtg
     6481 ggctcgaagg agcagtacct gctgcacctg cctcgaccga gcgtcaattt cggccacgcg
     6541 cgctggcgcg aga
//
LOCUS       DASPFR010000397         4420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1217084, whole genome shotgun sequence.
ACCESSION   DASPFR010000397 DASPFR010000000
VERSION     DASPFR010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217084"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1075)
                     /locus_tag="VD737_10570"
     CDS             complement(38..1075)
                     /locus_tag="VD737_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11053.1"
                     /translation="MRLPDSLRRLAYRLTGREPSVEDERLVALFRNRAELKKELTTLD
                     DERHRLLDRLKLQEGSTMRVEEQMTALEQYLGRPEEGYKSLAYFQLKALWRTAARRLE
                     QFGSELSRQQKDRERKAQVAAHDRDKRERMSAVERELVEARVLAEQLQAEQKLATQRL
                     EQLQGFWNYFRRRKLTDAIETRRQRIEAALTQVTDLTDSLHAIAAEPPPLFEGISVEG
                     RRAINLAVIGYAEMLHDRLAAGNLAELSRQSMLKRVYDAHYGSHAECQALMQRASQAA
                     AEIERLTDDLADIKARADRIRRTATYRSDTDVLPVADSLTAAANGGGTRRAAPNMLLD
                     EYWDIYKAVVR"
     gene            complement(1059..1487)
                     /locus_tag="VD737_10575"
     CDS             complement(1059..1487)
                     /locus_tag="VD737_10575"
                     /inference="COORDINATES: protein motif:HMM:NF018552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HXW11054.1"
                     /translation="MSLYESNYLRLRALVGDVRRHAGEQVSRVSGDCDLHLAVVEHAP
                     YTSTFKLTYRFEEQAGGTLADPDLELRVYHDARLAEVTACGRWIQHESLEHVREGIPR
                     QLGERWLRNMLLNKWLDYCLERGHAFVVAAGGARVHASPG"
     gene            complement(1691..2515)
                     /locus_tag="VD737_10580"
     CDS             complement(1691..2515)
                     /locus_tag="VD737_10580"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015269492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HXW11055.1"
                     /translation="MTRRTVFFVSDQTGVTAETMGHSLLTQFEGQEFRQVTLPFIATL
                     DKAEEAVRKINATGHEDGLRPIVFSTLVKEDLRRVVKTSNGLFLDFFYAFLGPLEGEL
                     EVKSSERAGRAHGIADQGAYVARIDATNFALAADDGAVSADYERADVILIGVSRSGKT
                     PTCIYMAMQYGVFAANYPFTEDDFDSKQLPAPVRGHTRKLFGLTIGPSRLQQIRNERR
                     PGSRYASIAQCEFEVRSAENLFHRHGIPSIDTTECSIEEIASRIIDRMGIERRLRP"
     gene            2590..>4420
                     /gene="ppsA"
                     /locus_tag="VD737_10585"
     CDS             2590..>4420
                     /gene="ppsA"
                     /locus_tag="VD737_10585"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HXW11056.1"
                     /translation="MRPYTLPFSRLGRNDVADVGGKNSSLGEMISNLAALGVTVPDGF
                     ATTADAYREFLAHEGLADRIKQELDALDVDDVAKLAETGAQIRDWIMSTPFPARLHQE
                     IVEGYARMCAGRKDDVAVAVRSSATAEDLPEASFAGQQETLLNVRGADNVVKAVHEVF
                     ASLFNDRAISYRVHQGFDHSQVALSAGIQYMVRSDLGASGVMFTLDTDSGFRDVVFIT
                     ASYGLGETVVQGAVNPDEFYVYKPALGVASHPILRRTLGAKAIKMVYAPPSQAEKRVL
                     TVDVPEAERMRFCISDADIIELAKQALVIERHYGCPMDIEWGKDGDTGQIYVLQARPE
                     TVQSRAGRTIQRYTLKQRGKVLASGRSIGQRIGAGPARVIKDVREMARVETGDVLVAD
                     MTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATRRIKEGQPVTVS
                     CAEGDTGYIYDGQLEFNQAQIELDSLPSLPVKIMMNVGNPDRAFDFAGIPNKGVGLAR
                     LEFIINRMIGVHPKALLEFDRLDPDLRDTIRRQMAGYDNPVEFYVEKLCEGIGTIAAA
                     FAPEPVIVRLSDFKSNEYAGLLGGAAFEVKEENPMIGLRGASRY"
BASE COUNT          718 a         1478 c         1479 g          745 t
ORIGIN      
        1 gccactaacc actagccact agccactagc cactcatcta gcggacgacc gccttgtaga
       61 tgtcccaata ctcatccagc agcatgttcg gcgcggcgcg gcgggtgccg ccgccgttgg
      121 ccgcggccgt gagcgagtcg gcgaccggca ggacgtccgt gtcgctgcgg tacgtcgcgg
      181 tgcgacggat gcggtcggca cgcgccttga tgtccgcgag gtcgtcggtg agccgctcga
      241 tctcggcggc cgcctgtgag gcccgctgca tcagcgcctg gcattcggca tgggacccgt
      301 aatgggcgtc ataaacccgc ttcagcatgc tctgccgcga cagctcggcc aggttgccgg
      361 ccgccagccg gtcgtggagc atttccgcgt agccgatcac ggccaggttg atcgcccggc
      421 ggccctcgac gctgatgccc tcgaacagcg gcggcggctc ggccgcgatc gcgtgcaggc
      481 tgtccgtgag gtcggtgacc tgcgtgaggg cggcctcgat gcgttgccgc cgcgtctcga
      541 tcgcatccgt gagcttgcgg cgacggaagt aattccagaa accctggagc tgctcgagcc
      601 gctgcgtcgc gagcttctgc tccgcctgca gctgctcggc cagcacgcgc gcctcgacga
      661 gctcgcgttc cacggccgac atgcgctcgc gcttgtcacg gtcgtgcgcg gcgacctgcg
      721 ccttgcgctc acggtccttc tgctggcgcg acagctcgga gccgaactgc tcgaggcgcc
      781 gcgcggccgt gcgccacagc gccttgagct ggaagtacgc gaggctcttg tagccttcct
      841 ccggacggcc gaggtattgc tcgagcgcgg tcatctgctc ctcgacgcgc atggtcgagc
      901 cttcctgcag cttgagccgg tcgagcaggc ggtgccgctc gtcgtcgagc gtcgtcagtt
      961 ccttcttgag ctccgctcga ttgcggaaaa gcgcgaccag tcgttcgtct tcgaccgagg
     1021 gttcgcgacc ggtgaggcgg tacgcgagcc ggcgcaggct atccgggaga cgcatgcacc
     1081 ctggccccgc cggctgcgac gacgaaggcg tggccgcgct cgaggcagta atccagccac
     1141 ttgttcagca acatgttgcg cagccatcgc tcgccgagct gtcgcgggat cccttcccgg
     1201 acgtgctcga ggctctcgtg ctggatccag cggccgcacg cggtgacctc ggcgagtcgc
     1261 gcgtcgtggt acacgcgcag ctcgaggtcg gggtccgcga gcgtgccgcc cgcctgctcc
     1321 tcgaagcgat acgtgagctt gaacgtcgag gtgtacggag cgtgctcgac caccgccagg
     1381 tgcaggtcgc agtcgccgga cacgcgcgag acctgctcgc ccgcatgacg tcggacgtcg
     1441 cccacgagcg cacgcagccg caggtagttg ctttcgtaga gcgacatcag gctcacgaag
     1501 ctcaccggcc ttgcgcgcca ggacgtgatg cagaggtggt ctagaaccat gaggggcaat
     1561 atagcagcga gcgccgggac cctgtccagt gtctagtgtt cagtgtctag cggttagcag
     1621 gaaggggtcg gcgtcaggtg gcacggacct tttcctacta accgctagac accaaacact
     1681 agacacttcc tcagggccgg agccgccgct cgatgcccat gcggtcgatg atgcggctgg
     1741 cgatctcctc gatcgagcac tcggtcgtgt cgatggacgg gatgccgtga cggtggaaga
     1801 ggttctcggc gctgcgcacc tcgaactcgc actgcgcgat cgacgcgtag cggctgccgg
     1861 gccgccgctc gttgcggatc tgctgcaggc gtgagggacc gatcgtcagg ccgaacagct
     1921 tgcgcgtgtg gccccggacg ggcgccggca gctgcttcga atcgaagtcg tcctcggtga
     1981 aaggatagtt ggcggcgaag acgccgtact gcatggccat gtagatgcac gtcggcgtct
     2041 tgcccgagcg cgacacgccg atcaggatca cgtcggctcg ctcgtagtcg gcagacaccg
     2101 cgccgtcgtc cgcggccagc gcgaagttcg tcgcgtcgat gcgggccacg taggcaccct
     2161 ggtccgcgat gccgtgcgca cggcctgcgc gttcgctcga cttgacctcg agttcgccct
     2221 cgagcggccc caggaaagcg tagaagaaat cgaggaacag cccgttgctc gtcttgacga
     2281 cgcgacgcag gtcttccttg accagcgtgc tgaaaacgat cggacgcagg ccgtcttcgt
     2341 gaccggtcgc gttgatcttc cttacggctt cctctgcctt gtcaagggtg gcgataaatg
     2401 gcagagtcac ctgtctgaat tcctgaccct cgaattgcgt gagcaggctg tgtcccatcg
     2461 tctcggctgt cacgccggtc tggtcagaga cgaaaaaaac cgttcgacgt gtcatctgct
     2521 cggcggttcc cgtggcgcga atttcgcgca gtctttcatc cctgtcgtcg atgtagcaag
     2581 gggcgcttga tgcgaccgta cacactcccg ttctcgcggc tgggccgcaa cgacgtcgcg
     2641 gacgtcggtg gcaagaactc ttccctcggc gagatgatct cgaacctcgc cgcgctcgga
     2701 gtgaccgtgc cggacgggtt cgcgaccacg gcggacgcat accgggaatt cctcgcgcac
     2761 gaagggctcg cggaccgcat caagcaggag ctcgacgcgc tcgacgtcga cgacgtcgcg
     2821 aagctcgcgg agaccggcgc gcagatccgc gactggatca tgtccacgcc cttccctgcc
     2881 cggctgcatc aggagatcgt cgagggctat gcgcgcatgt gcgccggccg caaggacgac
     2941 gtcgcggtcg ccgttcgctc gtcggcgacg gcggaagacc tgccggaggc ctcgttcgcg
     3001 ggacagcagg agacgctgct caacgtgcgc ggcgccgaca acgtcgtgaa ggcggttcac
     3061 gaggtcttcg cctcgctgtt caacgaccgc gcgatttcgt atcgcgtgca ccagggcttc
     3121 gaccattcgc aggtcgcctt gtccgcgggc atccagtaca tggtgcgcag cgacctgggc
     3181 gcgagcggcg tgatgttcac gctcgacacc gattccggtt tccgcgacgt ggtgttcatc
     3241 accgcgtcgt acggcctcgg cgagaccgtc gtgcagggcg ccgtcaatcc ggacgagttc
     3301 tacgtctaca agccggcgct cggcgtggcg agtcatccga tcctgcgccg cacgctcggc
     3361 gccaaggcga tcaagatggt ctacgcaccg ccgtcgcagg cggagaagcg cgtgctgacc
     3421 gtcgacgtgc ccgaagccga gcgcatgcgc ttctgcatct cggacgccga catcatcgaa
     3481 ctcgcgaagc aggccctcgt catcgagcga cactacggct gcccgatgga catcgaatgg
     3541 ggcaaggacg gcgacaccgg ccagatctac gtgctgcagg caaggcccga aaccgtgcag
     3601 agccgcgccg gccgcacgat ccagcgctac acgctcaagc agcgcggcaa ggtgctcgcg
     3661 agcggccgca gcatcggcca gcgcatcggc gcgggcccgg cgcgcgtgat caaggacgtg
     3721 cgcgagatgg cgcgcgtcga aaccggtgac gtgctggtcg ccgacatgac cgatccggac
     3781 tgggagccgg tcatgaagcg cgcgtccgcg atcgtgacga atcgcggcgg acgcacctgc
     3841 cacgcggcga tcatcgcccg cgagctcggc atcccggccg tggtcggctg cggcgacgcc
     3901 acgcgccgca tcaaggaggg ccagcccgtg accgtatcgt gcgccgaagg cgacacgggc
     3961 tacatatacg acggccagct cgagttcaac caggcccaga tcgagctcga ctcgctgccg
     4021 tcgctgccgg tcaagatcat gatgaacgtc ggcaatcccg accgcgcttt cgatttcgcg
     4081 ggcatcccga acaagggcgt cggcctcgcg cggctcgagt tcatcatcaa ccgcatgatc
     4141 ggcgtgcacc cgaaggcact gctcgagttc gaccggctcg acccggacct ccgcgatacg
     4201 atccgccggc agatggccgg ctacgacaac cccgtcgagt tctacgtcga gaagctctgc
     4261 gagggcatcg gcacgatcgc agccgcgttc gcgccggagc cggtgatcgt gcggctctcc
     4321 gacttcaagt cgaacgagta cgccggcctg ctcggcggag cggcgttcga ggtcaaggag
     4381 gagaacccga tgatcggcct gcgcggcgcc tcgcggtaca
//
LOCUS       DASPFR010000398         5460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_972184, whole genome shotgun sequence.
ACCESSION   DASPFR010000398 DASPFR010000000
VERSION     DASPFR010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972184"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..274)
                     /locus_tag="VD737_10590"
     CDS             complement(38..274)
                     /locus_tag="VD737_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11057.1"
                     /translation="MDFQRAMKNPGRTFGTPSVLEASTDFTVAQKRAILLQWKDQLTQ
                     LKVATEEGMPGPESDGAGAECLSAIVDALTRLGP"
     gene            403..762
                     /gene="arsC"
                     /locus_tag="VD737_10595"
     CDS             403..762
                     /gene="arsC"
                     /locus_tag="VD737_10595"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003120014.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HXW11058.1"
                     /translation="MTVTIYHNPGCGTSRNALALLRDSGIDPVVVEYLRDPPSRAQLQ
                     ALIRDAGLTVREALRQRGTPYDELGLGNPALSDDQLLDAMLAHPVLINRPFVVTPLGT
                     RLCRPLDRLHEILPPRR"
     gene            803..2335
                     /locus_tag="VD737_10600"
     CDS             803..2335
                     /locus_tag="VD737_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HXW11059.1"
                     /translation="MTMTTRTLTHRRPAERAGLLLARDLAGRAFSRAAGAPLVEGNGV
                     RLLIDARENYPAWLEAIAAAKDHVHFESYILYDDDVGRRFADALIEKARDGVRVRLIY
                     DWLGGLGKTSRAFWNRLRAGGVDVRCFNPPRLDSPVGWLSRDHRKSLTVDGNVGFVTG
                     LCVGRMWEGVPETKLELWRDTGVEIRGKAVADIERAFAQVWAMMGEPIPEDELTDPQS
                     LAPAGDVSVRIVASVPATAGLLRVDQLVAALARDRLWLSDAYYAGTTSYVQALRAAAQ
                     DGVDVRLLVPDATDIPLLRPLTRAGYRSLLEAGVRVFEWNGTMMHAKTAVADGQWARV
                     GSTNLNVASWLGNCELDVIVEDAPFAQSMEQAYLRDLENSTEVVLNERRRLRAPGAPR
                     RRRVLMTRGGGSGGRAAAGAIRIGHAIGAVFTDRRVLEPVEARIALVSGASLLALAIV
                     FAWFPRALVYPLVAVLVWVGVVLLYKGYRLYRDGRRGRISSKREEPTPGEATRRDGPS
                     SE"
     gene            2340..2792
                     /gene="tadA"
                     /locus_tag="VD737_10605"
     CDS             2340..2792
                     /gene="tadA"
                     /locus_tag="VD737_10605"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019406722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HXW11060.1"
                     /translation="MTSEDERFMRRAIELAREAETAGEVPVGALVVRDGEILAEGFNR
                     PISTHDPTAHAEMVALRAAAARIDNYRLTGTTLYVTLEPCAMCAGAMVHARVARLVYA
                     ANDPRAGAAGSVFNVVQSPALNHRLEVVPGVLAEDCGSLLRDFFVARR"
     gene            complement(2852..3220)
                     /locus_tag="VD737_10610"
     CDS             complement(2852..3220)
                     /locus_tag="VD737_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11061.1"
                     /translation="MKKIVATAMVMLGLFALTATAEATVLRVVVVETTDTAAYAAELN
                     KIRAVMTRLGSKATVRSWRTRFAGPDAGAVVVSLEYPDMATFAAEDAQAMADPEYRAL
                     LKGLDRLRKIVSDSLYEEIK"
     gene            complement(3332..4717)
                     /gene="mltF"
                     /locus_tag="VD737_10615"
     CDS             complement(3332..4717)
                     /gene="mltF"
                     /locus_tag="VD737_10615"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HXW11062.1"
                     /translation="MKVLLALITTLLLATCSQPPPLLQQILERGELRVVTRNSPDAYY
                     LGSQGPEGPAYDLASRFADQLGVPLRLYTVKTREAAIEEVAAGRAHVAAAGLSTGVIL
                     PKGTQFGPGYQRVREHLLYRRRGARPANILQAARGQIEVATGSAHLRTLEDLRLANPE
                     LAWVEREDTDTEEILAEVSQGAVQYTLATSTEFALNRSVHPELAIALDLSPERALAWV
                     VSTSGHDTTLLDRVNAYFALARREGAIAALLDRYYGKDERFDYLLSRNFMEHLESRLP
                     RYIAWFQEAAQEHGLDWRLLAAMGYQESKWDPAAVSHKGARGLMQITEDTASRVRVAD
                     RHDPKASIFGGARYVALLLKTIPRRIPDPDRTWFAVAAYNVGIGHVEDARILAQAQGR
                     NPDKWDEVRPFLPLLSQERWYTQTKRGYARGWEPVRYVENVQAYLNILQVAGLSVNSP
                     VAPPDPDVRNP"
     gene            4814..4906
                     /locus_tag="VD737_10620"
     tRNA            4814..4906
                     /locus_tag="VD737_10620"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4849..4851,aa:Ser,seq:cga)
     gene            5102..>5460
                     /locus_tag="VD737_10625"
     CDS             5102..>5460
                     /locus_tag="VD737_10625"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HXW11063.1"
                     /translation="MPLRTLGPEHILEVIQAAIAPVVLISGVGLLLLTLSGRLGRIVD
                     RTRIVASERNAASGAERAHLDRQLGVLGHRARLVRLALALSASSVAVSGLLITLLFLG
                     LLLEWNVTVACAVLFVTA"
BASE COUNT          773 a         1886 c         1902 g          899 t
ORIGIN      
        1 ggtcagcatg ggcggtggcg tttcggccgt cggggcgtca agggccgagc ctcgtcagcg
       61 catcgacgat ggcgctcagg cactccgcac cggcgccatc ggactccggg ccaggcatgc
      121 cttcctcggt cgccaccttg agctgggtga gctggtcctt ccactgcagc aggatggcgc
      181 gcttctgcgc gacggtgaag tcggtcgacg cctcgaggac ggacggcgtg ccgaacgtgc
      241 ggccgggatt cttcatcgct cgctggaagt ccatgacgcc ccctgcgggc cgcgactgca
      301 tcgttgattg gcgactcaat gatagtctgc tgccgtgttg cggcaagcct ctccctgaca
      361 ggcgccgcga cccgaccgga actccatgcc gaggaatcga cggtgaccgt cacgatctac
      421 cacaaccctg gctgcggcac ctcgcgcaat gcactggcac tgctgcgcga ttctggcatc
      481 gacccggtgg tcgtcgagta cctgcgggat ccgccgtcgc gcgcgcaact gcaggccttg
      541 atccgggacg cgggcctgac cgtgcgcgag gccctgcgcc agcgagggac gccatacgac
      601 gagctggggc tcggcaatcc cgcactcagc gacgaccagt tgctggacgc gatgctggcg
      661 cacccggtcc tgatcaaccg gccattcgtg gtgaccccgc tgggcacgcg cctgtgtcgt
      721 cctctggacc ggctgcacga gatcctgccg ccgcgccgct gaaggcgtcg tccggccgca
      781 gacgcttcaa tccggcgccc cgatgacgat gacgacgcgc accctgacgc accggcgacc
      841 ggcggaacgg gcgggcctgc tgctcgcgcg cgatctcgcc ggccgggcgt tttcgcgcgc
      901 agccggagcc ccgctggtcg agggcaacgg cgtgcgtctg ctgatcgacg cccgcgagaa
      961 ttatcccgcg tggctcgagg cgatcgcggc cgcgaaagat cacgtgcact tcgagagcta
     1021 catcctctac gacgacgacg tcggccgcag gtttgccgac gcgctgatcg agaaggcccg
     1081 ggacggcgtc cgcgtgcggc tgatctacga ctggctcggc gggctcggca agacgtcgcg
     1141 cgcgttctgg aaccggttgc gcgccggcgg cgtggacgtg cggtgcttca accccccgcg
     1201 cctcgattct ccggtcggct ggctctcgcg cgaccatcgc aagtcgctca cggtcgacgg
     1261 aaacgtcggt ttcgtgacgg ggctctgcgt cgggcggatg tgggaaggcg tgcccgagac
     1321 aaagctcgag ctgtggcgcg acaccggggt cgagatccgc ggcaaggccg ttgcggatat
     1381 cgagcgcgcg ttcgcacagg tctgggcgat gatgggcgag ccgatccccg aggacgagct
     1441 gaccgacccg cagtcgctcg cgccggccgg ggacgtgagc gtgcggatcg tggcgagcgt
     1501 gccggcgacc gcagggttgc tgcgcgtgga ccagctcgtc gctgcactgg cccgcgaccg
     1561 actctggctc agtgacgctt attacgccgg caccaccagc tacgtccagg ccttgcgggc
     1621 tgccgcgcag gacggcgtgg acgtgcggtt gctcgtcccg gacgctaccg acatcccgct
     1681 gctgcgaccg ctgactcgcg cgggctatcg ctcgctgctc gaggccggcg tccgcgtctt
     1741 cgaatggaac ggcacgatga tgcacgccaa gacggccgtg gccgacggac aatgggcgcg
     1801 cgtgggctcg acgaacctga acgtggccag ctggctcggc aactgcgaac tggacgtgat
     1861 cgtcgaagac gcgccctttg cgcagtcgat ggagcaggcg tacctgcgcg acctggaaaa
     1921 ctcgacggag gtcgtcctca acgaacgacg ccggcttcgt gcaccgggcg cgccgcgtcg
     1981 ccgccgcgta ttgatgacgc gcggtggcgg gagcggcggg cgggccgcag ccggcgcgat
     2041 tcgcatcggc cacgccatcg gcgcggtgtt cacggatcgg cgcgtgctcg agcccgtcga
     2101 ggcacgcatc gcgctggtgt cgggcgcctc gctgctggcg ctcgcgatcg tcttcgcgtg
     2161 gttcccgcgc gcgctggtct atccgctcgt ggcggtgctc gtgtgggtcg gcgtcgtact
     2221 gctgtacaag ggataccgcc tgtaccgcga cggccggcgg ggtcgaatct cgtcgaaacg
     2281 cgaggagccg acgcccggcg aggccactcg ccgggacggc ccgtcgtcgg agtgagcctg
     2341 tgaccagcga ggacgaacgc ttcatgcgcc gtgcgatcga gctcgcacgg gaggccgaga
     2401 ccgccggcga ggtgcccgtc ggcgccctgg tcgtgcgcga cggcgaaatt ctcgccgaag
     2461 ggttcaaccg cccgatctcc acgcacgacc cgactgccca cgccgagatg gtcgcgctcc
     2521 gcgcggccgc agcccgcatc gacaattacc gcctgaccgg cacgacgctg tacgtgacgc
     2581 tcgagccgtg cgcgatgtgc gcgggcgcga tggtccacgc ccgcgtggcc cggctggtgt
     2641 atgcggccaa cgacccgcgc gcgggtgcgg ccggcagcgt cttcaacgtc gtgcagagtc
     2701 ccgcgctcaa ccaccgcctc gaggtcgtgc cgggcgtgct cgccgaggac tgcggttcgc
     2761 tgttgcgcga cttcttcgtc gcccgtcgct aggaggcggg gtcagcgctg acgttctcga
     2821 cgcgcgtcca gacgaagtgc aggcgcgtcg gctacttgat ctcctcgtag aggctgtccg
     2881 acacgatctt ccgcagtcgg tccagtccct tcagcagcgc ccggtattcc ggatcggcca
     2941 tcgcctgcgc gtcctcggcc gcgaacgtag ccatgtcggg atactcgagc gacacgacga
     3001 cggcgccggc atccggccct gcgaagcgcg tccgccacga gcgcacagtg gccttgctgc
     3061 cgagcctcgt catcaccgcc cggatcttgt tcagctcggc cgcgtaagcg gcggtgtccg
     3121 tcgtttccac caccaccacg cgaagcacgg tggcttccgc cgtggccgtc agggcgaaca
     3181 acccgagcat gaccatcgcc gtcgccacga ttttcttcat gatgtctccc tcctgccaat
     3241 ttctgtgcag ttgtgatcgc gtgcgcgccg atcctgcgct cgcgagcgat ccggcgtcaa
     3301 accagcggtc gctcgtccgc agtcacctcc gctatggatt gcgaacgtcc ggatcgggcg
     3361 gggcgaccgg agaattcact gacaggcccg cgacctgcag gatgttcagg tacgcctgga
     3421 cgttctcgac gtaacggacg ggctcccatc cgcgcgcata gccgcgcttg gtctgcgtgt
     3481 accagcgctc ctggctcagc aggggcagga acgggcgcac ctcgtcccac ttgtccgggt
     3541 tgcgcccctg cgcctgcgcg aggatgcgcg cgtcctcgac gtggccgatg ccgacgttgt
     3601 aggcggccac cgcgaaccac gtgcggtccg ggtcgggaat gcggcgggga atcgtcttga
     3661 gcagcagcgc aacgtacctc gcgccgccga agatgctcgc cttcgggtcg tggcggtcgg
     3721 ccacgcgcac gcgcgaggcc gtgtcctcgg tgatctgcat cagcccgcgc gcgcccttgt
     3781 gcgagacggc cgcggggtcc cacttcgact cctggtatcc catggccgcg agcagtcgcc
     3841 agtccaggcc atgctcctgc gcggcttcct ggaaccacgc gatgtagcgc ggcagccgcg
     3901 actcgaggtg ttccatgaag ttgcgcgaga gcaggtagtc gaagcgctcg tccttgccgt
     3961 agtagcggtc gagcagtgcg gcgatggccc cttcgcgacg cgcgagcgcg aagtacgcgt
     4021 tgacgcggtc gagcaacgtc gtgtcgtgac ccgacgtgct gacgacccac gcgagcgccc
     4081 gctcgggcga gaggtccagc gcgatcgcga gttccgggtg caccgagcga ttgagcgcga
     4141 actcggtcga ggtagccagc gtgtactgca cggccccctg cgagacttct gcgaggatct
     4201 cctcggtgtc ggtgtcctcg cgctcgaccc aggcgagttc cgggttcgcg aggcgcagat
     4261 cctccagtgt ccgcaggtgc gcgctgccgg tggccacctc gatctggccg cgcgccgcct
     4321 ggagaatatt cgcgggtcgc gcaccgcgac ggcgatacag caggtgctcg cgtacccgct
     4381 ggtacccggg gccgaactgg gtgcccttcg gcaggatcac gccggtggac aggcccgcgg
     4441 cggccacgtg cgcccgtccc gccgcgacct cctcgatcgc ggcttcgcgc gtcttgacgg
     4501 tgtacagccg cagcggcacg ccgagttgat cggcgaagcg gctcgcgagg tcgtacgcgg
     4561 gaccttcggg cccctggctg ccgaggtagt acgcgtcggg gctgttgcgg gtgacgacgc
     4621 ggagttcgcc ccgctcgagg atctgctgca gcaacggcgg cggctggctg cacgtggcca
     4681 gcaggagggt cgtgatcagt gccaggagga ctttcatgca gggcgcgggc cgccgcgcat
     4741 tgtgccctcg gccatgggtc ggtgctcggg tggtgctcca cggcgtgctg ctatagaatg
     4801 cgcggccctc tacggagagg taccgaagcg gtcataacgg cgccgactcg aaatcggatg
     4861 ggggcataac agccccacgt gggttcgaat cccaccctct ccgccagatc ccagctggac
     4921 gacgcccccc gaccggggcg tttttcgttt cggtcctcgc tccgggctac ggcgacgtgg
     4981 cctcggccac gcggatcatg accagcgctc acccatcacc gtacgagaat tcagcctcgt
     5041 cttgtggtga tcccgtaccg gcgcgtgacg gtcgcggtgc acgcgcgggc tatagtgaat
     5101 catgcccctg cgaaccctcg gccccgagca catcctggag gtgatccagg ccgcgattgc
     5161 ccccgtggtg ctgatctcgg gcgtcggact gctgctgcta accctgagcg gccgcctcgg
     5221 acgcatcgtc gatcgcacgc gcatcgtggc gagcgagcgc aacgccgcga gcggcgctga
     5281 gcgcgctcac ctcgaccggc aactcggggt actgggtcac cgcgccaggc tcgtgcggct
     5341 cgcgctcgcg ctcagcgcct cgtcggtcgc cgtgagcggt ctgctgatca cgctgctgtt
     5401 cctcggcctt ctgctcgagt ggaacgtcac cgtcgcgtgc gccgtgctgt tcgtaaccgc
//
LOCUS       DASPFR010000399         3380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_485521, whole genome shotgun sequence.
ACCESSION   DASPFR010000399 DASPFR010000000
VERSION     DASPFR010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485521"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..400)
                     /locus_tag="VD737_10630"
     CDS             complement(128..400)
                     /locus_tag="VD737_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11064.1"
                     /translation="MNLKSRLLRWWDSLLGRPPWYRDSNVTISRTDLYQDGASKTPPK
                     PQPGELTLVDDEAADKGRRRRTAGVDPYSNDAGFAKPHGWDRVDRD"
     gene            complement(397..663)
                     /locus_tag="VD737_10635"
     CDS             complement(397..663)
                     /locus_tag="VD737_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11065.1"
                     /translation="MLAAFAFGVLVLPFFVYVTGTYVFGRYAAGGALGFFGDFARGLA
                     GFSWHAWALALGPVAIVSVWRALWRLSSPSRSPPPPPLRRREPT"
     gene            847..1800
                     /locus_tag="VD737_10640"
     CDS             847..1800
                     /locus_tag="VD737_10640"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXW11066.1"
                     /translation="MTMRKVRTAVIGVGYLGRFHAQKYASLPQSHLVGVVDASAEHRD
                     RVAAETQCRAYADYRDLLDGVDAVSIATPTPLHYPIARECLERGVHVLVEKPITTTLE
                     EARSLVDVATRTGRILQVGHLERFNAAILALQGTLGRPRFVESHRLAPFKERGTDVNV
                     VLDLMIHDIDLIQSLVGSPIVSIDAVGTSVFSAGLDIANARIRYASGCVANTTASRVS
                     MKMERKLRLFQDDAYISIDLQQKVLTIVRKPPAGSDAPRGQVVIEERSYEQGDALRHE
                     IEAFLQSIVDGTAPVVTGEDGLRALETATRITQMVQEAPRS"
     gene            1797..2966
                     /locus_tag="VD737_10645"
     CDS             1797..2966
                     /locus_tag="VD737_10645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HXW11067.1"
                     /translation="MSRRYYAGRDFRRALSIEELRRVALRRLPRFEAEYLEGGAEDEV
                     ALRRNRTIFERINWVPRALVGTGIPDLSRQVLGDALHMPVVIAPTGFNGMLWPDGDLA
                     LARAADASGIAFTLSTVGNYEVGALNAQLKTPSWFQLYPLKDTKTSDRIVDKAQEAGC
                     TKLVVTVDVPALGAREWDQRNYRRPLKLSASSVLDVLMHPRWVTQVMLPKGAPKFANI
                     AEFLPPGAQSALQGVRFMGTQINPGLCWADMERMRGRWKGKFVLKGLLSVEDAQRAVE
                     IGCDGIVLSNHGGRQLDSCVTGIEVVGQVADAVGDRLAILVDGGFRRGNDVLKAIALG
                     ADAVMLGRAPLYGLAAGGSAGVEHSLSLLRMEMERALTLLGCHSLDELGPQLIRA"
     gene            2968..>3380
                     /locus_tag="VD737_10650"
     CDS             2968..>3380
                     /locus_tag="VD737_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HXW11068.1"
                     /translation="MIAIREAWQAGLLRREHWLRNVVAGFVVGVVALPLAMAFAIASG
                     AKPEQGLYTAIVAGAAVSLFGGSRLQIAGPTGAFVVILAGVTVQHGIDGLQIATFMAG
                     VMLLALGLTRLGGIIRFIPDPVITGFTAGIGVIIFV"
BASE COUNT          532 a         1157 c         1180 g          511 t
ORIGIN      
        1 tagtggctag tggctagtgg ccaggcagcg cctgcggcgt cttcggacag ctggacgcgc
       61 cctcccgggt tacgcccttc cgggttacta gccactaacc actagccact ggccactggc
      121 acttcaatca gtcccggtcg acccggtccc atccatgggg cttcgcgaac ccggcgtcgt
      181 tcgagtatgg atcgacgccg gcggtgcgcc ttcgccgccc cttgtcggcc gcctcgtcgt
      241 cgaccagcgt cagttcgccg ggctgcggct tgggcggtgt cttcgacgcg ccatcctggt
      301 agaggtcggt gcggctgatc gtgacgttcg aatcgcggta ccacggtggt cgaccgagca
      361 gcgagtccca ccagcgcagc agtcgtgact tcaggttcat gtcggttccc gccggcgcaa
      421 tggcggtggc ggcggcgagc gcgacggcga cgagagccgc cacaacgcgc gccagacgct
      481 cacgatcgcg accgggccga gcgcgagcgc ccaggcgtgc cagctgaagc ccgcgagccc
      541 gcgggcgaag tcgccgaaga atcccagcgc gcctccggct gcatatcggc cgaacacgta
      601 ggtccccgtc acgtagacga agaagggcag cacgagcacg ccgaaggcga atgcggcgag
      661 catcaggtac ctgtcgtgac gcggcgacca gtgcatggga agtgaagtaa ccagtgatca
      721 gtgaacggtg accagtgacc agtcggcaaa atcaggacga ccgcatcgtg agcataagcc
      781 gtatccgccg caagtcccgc cattgagtat catgactgga tcacgttggg aggggccggg
      841 tcggtcatga cgatgcgcaa ggtgcggacg gccgtgatcg gggtgggcta cctcggccgg
      901 ttccacgcgc agaagtacgc ctccctgccg cagtcgcacc tcgtcggcgt cgtcgacgcc
      961 agcgccgagc accgcgaccg cgtcgcagcc gagacgcagt gccgggccta cgccgactac
     1021 cgcgacctgc tggacggggt ggacgccgtc agcatcgcca cgccgacgcc gctgcattac
     1081 ccgatcgcgc gagagtgcct cgagcgcgga gtgcacgtgc tggtcgagaa gccgatcacg
     1141 accacgctcg aggaggcccg cagcctggtc gacgtcgcga cgcgcaccgg gcgcatcctg
     1201 caggtgggcc acctcgagcg cttcaacgcc gcgatcctcg cgttgcaggg cacgctcggg
     1261 cgcccgcgct tcgtcgaatc gcaccggctc gcgccgttca aggaacgcgg caccgacgtc
     1321 aacgtcgtgc tggacctcat gatccacgac atcgacctga tccagagcct ggtcggctcg
     1381 ccgatcgtct cgatcgacgc ggtgggcacg tcggtcttct cggccgggct cgacatcgcc
     1441 aacgcgcgca tccgctacgc gagtggctgc gtcgccaaca cgactgccag ccgcgtgagc
     1501 atgaagatgg agcgcaagct ccggctgttc caggacgacg cctacatctc gatcgacctg
     1561 cagcagaagg tgctgacgat cgtgcgcaag ccgccggccg gctccgacgc cccgcgcggg
     1621 caggtcgtga tcgaggaacg cagctacgag cagggcgatg cgctccggca cgagatcgag
     1681 gctttcctgc agtcgatcgt cgacggcacg gcgccggtgg tcaccggcga ggacggactg
     1741 cgcgcgctcg agacggcgac gcggatcacg cagatggtgc aggaagcgcc ccgctcatga
     1801 gccgccgcta ctacgccggg cgcgacttca ggcgcgcgct gtcgatcgag gaactgcgtc
     1861 gcgtcgcgtt gcggcgcttg ccgcgcttcg aggcggagta cctcgagggc ggtgccgagg
     1921 acgaggtcgc gctgcgacgc aaccgcacga tcttcgagcg gatcaactgg gtgccgcgtg
     1981 cgctcgtggg cacgggcatc ccggacctct cgcggcaggt gctcggcgac gcgctgcaca
     2041 tgccggtcgt gatcgcgccg accggcttca acggcatgct gtggccggac ggcgacctcg
     2101 cgctcgccag ggcggccgac gcctcgggca tcgcgttcac gctgagcacg gtcggcaact
     2161 acgaagtcgg ggcgctgaac gcgcagctga agacaccgtc ctggttccag ctctacccgc
     2221 tcaaggacac caagacctcg gatcgcatcg tcgacaaggc acaggaggcc ggctgcacca
     2281 agctggtcgt caccgtggac gtgccggcac tcggcgcgcg cgaatgggac cagcgcaact
     2341 accgacgccc gctcaagctg agtgcctcga gcgtcctcga cgtgctgatg catccacgct
     2401 gggtcacgca ggtgatgctg ccgaagggcg cgccgaagtt cgcgaacatc gccgagttcc
     2461 tgccgcccgg cgcgcagtcc gccctgcagg gcgtgcggtt catgggcacc cagatcaacc
     2521 cgggactgtg ctgggcggac atggagcgca tgcgcggccg ctggaagggc aagttcgtgc
     2581 tcaaggggct gctgagcgtc gaggacgcgc agcgtgccgt cgagatcggc tgcgacggca
     2641 tcgtgctgag caaccacggc ggacgccagc tcgattcgtg cgtgaccggc atcgaagtcg
     2701 tgggacaggt cgccgacgcc gtgggcgacc gactggcgat cctggtcgac ggcggattcc
     2761 gccgcggcaa cgacgtgctc aaggcgatcg cgctcggcgc cgacgcggtg atgctcgggc
     2821 gcgcgccgct ctacgggctg gccgccggcg gctccgccgg ggtcgagcat tcgctcagcc
     2881 tgctgcgcat ggagatggag cgcgccctga cgctgctcgg ctgccattcg ctcgacgagc
     2941 tcggcccgca attgatccgc gcctgacatg atcgcgatcc gcgaggcctg gcaggccggg
     3001 ttgctcaggc gtgagcactg gctgcgcaac gtcgtcgcgg gcttcgtcgt cggcgtcgtc
     3061 gcactgccgc tcgcgatggc gtttgcgatc gcttcgggcg ccaagccgga gcaggggctg
     3121 tacacggcga tcgtcgcggg cgccgccgtc tcgctgttcg gcggcagccg cctgcagatc
     3181 gccggcccga ccggggcgtt cgtggtgatc ctggcgggcg tcacggtgca gcacggcatc
     3241 gacggcctgc agatcgcgac cttcatggcc ggcgtgatgc tgctcgccct ggggctcacg
     3301 cgcctcggcg gcatcatccg cttcatcccg gaccccgtga tcaccggctt cacggccggc
     3361 atcggcgtga tcatcttcgt
//
LOCUS       DASPFR010000400        10968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_729195, whole genome shotgun sequence.
ACCESSION   DASPFR010000400 DASPFR010000000
VERSION     DASPFR010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10968
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729195"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1581)
                     /gene="cysG"
                     /locus_tag="VD737_10655"
     CDS             complement(151..1581)
                     /gene="cysG"
                     /locus_tag="VD737_10655"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HXW11069.1"
                     /translation="MRYFPLFADLRDRRVLVVGGGVVAERKARLLLDAGADVSVVAPE
                     FSASLLQLAEEGRGEGNLILLQEPFRPEHLDGVVLAVAATDDAAVNAAVAGAGRERNL
                     LVNVVDVAELSSFIVPAIVDRSPLVIAISSGGVAPVLARHVRERIESLVDGSVGRLAG
                     LLERWRARIKQQRPDVSSRRRFYERLLRGRVADLVRVRDEAAAERELERELGRREATP
                     RGTVALVGAGPGDAGLLTLNGLRALQEADVVMHDRLVSPEVLDLARRDALKIAVGKSA
                     RDHNSVSQSRIHELMLEHAAAGRRVVRLKGGDPFIFGRGGEELEFLEKHGVPFEVVPG
                     ITAALACGAYAGIPLTHREHAQSVRLVTAHCGASFDALDWPALAQERETVAFYMGVAG
                     LERIRTSLVAHGRSPRTPVAIVENGSRPTQRVVLATLGELDEAGSTAQVGSPALVIVG
                     EVAALAARLHWFGRAPIEWHRLKQAA"
     gene            complement(1587..2318)
                     /locus_tag="VD737_10660"
     CDS             complement(1587..2318)
                     /locus_tag="VD737_10660"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HXW11070.1"
                     /translation="MRALAWNPETAMHEPAVLAEINRDLERRSAEDRVRWALEHLPGE
                     HALSSSFGAQAAVSLHMVTRQKPCLPVVLVDTGYLFPETYRFIDDLVEKLELNLRVYQ
                     PRMSAAWLEARYGRLWEQGVEGIERYNDLRKTEPMRRALDELHVNTWFAGLRRDQSQS
                     RSRIGPIEWRDGRWKVHPIFDWNDRQVFDYLRAHSLPYHPLWDEGYVSIGDWHTTRTL
                     AEAGDAEATRFFGLKRECGIHGLEG"
     gene            complement(2341..4011)
                     /gene="cysI"
                     /locus_tag="VD737_10665"
     CDS             complement(2341..4011)
                     /gene="cysI"
                     /locus_tag="VD737_10665"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF010029.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HXW11071.1"
                     /translation="MTELSHVEKIKRESRQLRGTLDESLRDSLTGAIREDDTVLIKFH
                     GSYQQDDRDIREERRKQKLEPAYSFMIRTRLPGGVCTPQQWLQLDEIARRYANGTLRI
                     TTRQAFQFHGVIKTELKSTMQAINAALIDTIAACGDVNRNVLASANPVETRAHPVVAQ
                     WATKLSEHLLPRTRAYHEIWLDGEKVVGTPEADPLYGEAYLPRKFKTAIVVPPVNDVD
                     VFAHDLGFIAIVEDGELQGFNVTIGGGMGASHGDPETYPRLADVVGFVEPGQLLATAE
                     AVLTTQRDFGNRAVRKRARLKYTIDDRGLDWFVAEVTRRQGFEFQPARPFTFTQQGDR
                     FGWIEGYDGRWHLTVRIEAGRVADTERAKQLTGLREIAKVHRGDFRLTPNQNLTIANV
                     DASARAFISALVVKHGLNRHEQASPVARDALACVALPTCGLAMAEAERYLPRLVKLVE
                     DRLAAHGLAGEPLVLRITGCPNGCARPYLAEVALVGKAPGRYNLFYGGDGRGERLNAL
                     HLENVDEPTILSALDSAFGRYAQERAPGERFGDFAWRAGLVGSAAPAA"
     gene            complement(4030..5853)
                     /locus_tag="VD737_10670"
     CDS             complement(4030..5853)
                     /locus_tag="VD737_10670"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HXW11072.1"
                     /translation="MSANIVSAFPLGPLDPERSELLLRVVEGLEPSSLQWLSGFAAGV
                     AHERAANRAGAVAPAPAARPESAARLTIVYGSQTGNGKRIAERLGRSAEAAGLAARVY
                     SSGNYPLKDLPKERLLVVVVSTQGDGDPPDDARGFLEFLASRRAPKLEQLSYSVLALG
                     DSSYPKFCETGKQVDDRLAALGARRLLDRVDCDVDYDTLATPWLDRVVTTARDTLGAS
                     PQVATVTRLRAPAREPQHSREQPFAAEVLTNQRITARDATKDVRHVEFALEGSGLTYA
                     PGDALGVWHENPAPVVDEVLQALRLDGTQDVELDGQARTLREWLTGSREITRLTRPFL
                     VQQAERSKDPALAAVLQPGHEQALRSTLKDLQVVDVLQRHRGDWDARGFVQALRPLAP
                     RLYSIASSPEAVGEEAHVTVAVVEYEHAGQRRLGAASSYLAGLPAESARARVFIEHNE
                     RFRLPNDASRDVIMIGPGTGVAPFRGFLQHREAQGATGRNWLFFGARHFQSEFLYQVE
                     WQDAVRKGVLTRLDAAFSRDRSTPRTYVQDRLRAAGKEVYRWLEGGAYLYVCGDAEQM
                     APDVQAALVDLVAEHGGRRREDAEAYVRALADERRYLRDVY"
     gene            complement(5961..6917)
                     /locus_tag="VD737_10675"
     CDS             complement(5961..6917)
                     /locus_tag="VD737_10675"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXW11073.1"
                     /translation="MSTVALHFRDFDLPWTRNFPEERRFRRMLAAALGLFVGFGIVIP
                     FLPVTPPSQAVAPAVPERVLEFILEQPKPRPAPEPQPQPKIEVPVPEPIDASRPEVKT
                     PTAKPLARSEPARDPRRKAAASGLVAMADQLAELRNLDLAKDVPAQAIDTGATQRSSV
                     DRSILTARANAGSGGIAVGAASRGFGGGAVGLKGVSTTTVASGTATAAESAPEAQRSG
                     RSAKAARTREEIELIFDKNKSAIYALYSRALRENPALQGKVVLEVTIAPSGDVTECRV
                     VSSDLGDAELERKLVARVKLFRFEARDVAVMTTTKPIEFFPA"
     gene            complement(6927..7442)
                     /locus_tag="VD737_10680"
     CDS             complement(6927..7442)
                     /locus_tag="VD737_10680"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXW11074.1"
                     /translation="MIRVGLRRHTRRGKGGAGGGHGPLALIPMIDMLTILVVYLLVHA
                     ADMDILPNSKNIQIPQSSSEQKPREATVVMVTRDMLYVNGEAVVTVAELGATNGRVNE
                     PLRAALGSQSAGVLPGSEAHREVTVMAEKSLPYAVLRRIVASSSAADYTKVSFVVVER
                     EQAYRRGATGA"
     gene            complement(7439..7948)
                     /locus_tag="VD737_10685"
     CDS             complement(7439..7948)
                     /locus_tag="VD737_10685"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXW11075.1"
                     /translation="MRRGSALARRQQRNHARYRGRGINIVPMLDVMVILTFFLIFTAV
                     FSKTSVLEVNLPVPGTQAAATPPSFELELILRRSGIDVAERSTGVLATIPAAATGQDV
                     AALTEYLAGLKQRVPEVTSATLLVADDVDYDTIVQVMDATRVRQVVDGARIVRESLFP
                     QVALGDAPT"
     gene            complement(7945..8691)
                     /locus_tag="VD737_10690"
     CDS             complement(7945..8691)
                     /locus_tag="VD737_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXW11076.1"
                     /translation="MSTVDLIVNFFKQGGPFLYPIAGVFVIGLVLAVERFIYLTRVSR
                     GNRRLWAKLVPLLQQGNFRKALELAEQTDSALSSVMRYGLSRAANAQRREDVEKAMEE
                     SLIEVIPRLEKRTQALAGLANVGMLMGLLGTVIGLIHAFAAVAAVNPAEKASMLSASI
                     SVAMNNTALGLGLAITLLLCHMYLEMRTTNLVDSLEVASIKFLNTVFEARDSGLQAYS
                     QSVAGGATAAAAQRKGGRVPDDVLREAARA"
     gene            complement(8688..9125)
                     /locus_tag="VD737_10695"
     CDS             complement(8688..9125)
                     /locus_tag="VD737_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11077.1"
                     /translation="MTMLDPLRPVTGEPLPAARARNTCRWLASLAAIAVCASPVRASE
                     PPVEPVVSATAKSGERAVDRLHLDATSITGNRELPKVMSIVPWKAAEPPAGPDRPMGS
                     LIEELLTPLDRDEFRREIAYHRDLTSRTAPVAPHVEVPGVSQP"
     gene            complement(9122..>10968)
                     /locus_tag="VD737_10700"
     CDS             complement(9122..>10968)
                     /locus_tag="VD737_10700"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXW11078.1"
                     /translation="LLLADLLFEGQRFAEAAVEYDRAAYGYPNAAEAARAGYAVLVAL
                     DKAALQMTDPDRLRLNERVVEASLKFAEAFPAHTETPPVLTRACKLVFDSDDRERAES
                     LAQRVLGLGPRATPEQQRVAWTVLAHTYFDSSRFAEAERAYAEVVARLSRDDPQYAES
                     VERRAASIYRQAEVRRAAGDVDGAVQDFLRIGVVAAASPIRANAEFDAATLLLEARRW
                     PQAAQVFEQFRRDHPGHALARQVEARLAVAYVESGESTRAAAEYERVAARPDEEAEVR
                     RAAQWQAAELYAGAGDRVGATRAYGVYVERFPVPAAPAIEARQTLADYARDANDGLGR
                     RRWLEALVAADAVAGAERSDRTRLLAATATLELARPLDAQARAIRLALPLDRSLAAKR
                     EALQAALATYGRAESYGIAQVTTAAAYAMADLYRELGRSLLESDRPDDLDDDELEQYE
                     VLLEEQAFPFEEKAIALHERNARHAAQGIYDEWVQRSYVDLAQLEPARYARDEVVAAP
                     AEDVVAPESDPAALNQLAVARRRAGAFEEAREAYERALALDPGYADAERNLGILHDLY
                     LDDPVAALPHYERYQALKQGADVQVAAWLVELRTRLSTVTRTAEVATP"
BASE COUNT         1568 a         3835 c         3909 g         1656 t
ORIGIN      
        1 cgagcgtgat gtctccgatc atccttgacc ccatgacgac gtttggtgcg ggcatcgtgg
       61 cacccggcgg tggcgctgag gtggggatta tgcgcccgct gccctcacgg ccttcggccg
      121 accccttcca gagaaccggg agaggtggag tcaggcggcc tgcttgaggc ggtgccactc
      181 gatcggcgcg cggccgaacc agtgcagccg ggcggcgagc gccgccacct cgccgacgat
      241 cacgagcgcg ggcgagccca cctgcgccgt gctgccggcc tcgtcgagtt cccctagcgt
      301 cgccaggacc acgcgctgcg tcggtcgtga accgttctcc acgatcgcca ccggcgtgcg
      361 gggcgagcgc ccgtgtgcga cgagcgacgt acggatgcgt tcgagccccg cgacccccat
      421 gtagaacgcg acggtctcgc gctcctgcgc gagcgccggc cagtccagcg cgtcgaacga
      481 cgcgccgcag tgcgccgtga cgagtctcac ggactgcgcg tgctcgcgat gcgtgagcgg
      541 aatgcccgcg tacgcgccgc aggccagggc cgccgtgatg ccgggcacga cctcgaacgg
      601 gacgccatgc ttttcgagga attcgagctc ctcgccgccg cggccgaaga tgaacggatc
      661 gccacccttc agccgcacca cgcggcgccc ggccgcggcg tgttcgagca tcaattcgtg
      721 tatgcgggac tgcgacaccg aattgtggtc gcgcgccgac ttgcccactg cgatcttcag
      781 cgcgtcccgt cgggccaggt ccagcacctc cggtgagacc agccggtcgt gcatcacgac
      841 gtcggcctcc tgcagcgcgc gcaggccgtt cagcgtgagc agcccggcgt cgccgggccc
      901 ggcgccgacg agcgcgacgg tgccgcgcgg cgtggcctcg cgccggccga gctcgcgctc
      961 gagctcacgt tccgccgcgg cttcgtcgcg aacccgcacg aggtccgcca ccctgccgcg
     1021 caagagccgt tcgtagaagc gtcgccgcga cgacacgtcc ggccgctgct gcttgatccg
     1081 cgcccgccag cgctcgagca ggccggcgag tcggccgacc gagccatcga cgagcgattc
     1141 gatgcgctcg cgcacgtgcc gcgcgaggac gggcgcgacg ccgcccgagg aaatcgcgat
     1201 gaccagcggc gaccggtcga cgatcgccgg cacgatgaag ctcgagagtt ccgccacgtc
     1261 gacgacgttc acgaggaggt tgcgctcgcg cccggcgccg gccaccgcgg cgttgaccgc
     1321 ggcatcgtcg gtcgccgcga ccgcgagcac gacgccgtcg aggtgctcgg gacggaaggg
     1381 ctcctggagc agaatcagat tgccctcacc ccgcccttct tccgcgagct gcaggagcga
     1441 ggcactgaac tccggggcga cgacgctgac gtccgcgccg gcgtccagca gcagccgcgc
     1501 cttgcgctcg gccacgacgc caccaccgac gacgagcacg cggcggtcac gcaggtcggc
     1561 gaagagcggg aaatagcgca ctcgaatcag ccctcgagac cgtggatgcc gcactcgcgc
     1621 ttgagcccga agaagcgcgt ggcctcggcg tcgcccgcct ctgcgagcgt gcgcgtcgtg
     1681 tgccagtcgc cgatcgacac gtaaccttcg tcccagagtg ggtgatacgg cagcgagtgc
     1741 gcccgcaggt agtcgaagac ctgccggtcg ttccagtcga agatcgggtg gaccttccag
     1801 cggccgtcgc gccactcgat cggcccgatg cggctgcggc tctgggactg gtcgcgacgc
     1861 aacccggcaa accaggtgtt gacgtgcaac tcgtcgaggg cgcgccgcat cggctcggtc
     1921 ttgcgcaggt cgttgtagcg ctcgatgccc tcgacgccct gttcccagag ccggccatag
     1981 cgcgcttcga gccacgccgc cgacatgcgc ggctggtaga ccctgaggtt cagttcgagc
     2041 ttctcgacca ggtcgtcgat gaaacgatag gtctcgggaa acaggtagcc ggtgtcgacc
     2101 agcacgaccg gcaggcacgg cttctgtcgg gtgaccatgt gcagcgacac cgccgcctgc
     2161 gcaccgaagc tcgacgagag ggcgtgctcg cccggcaggt gctcgagcgc ccaccgcacg
     2221 cgatcctcgg cgctgcgacg ctcgaggtcg cggttgatct cggccagcac ggccggctcg
     2281 tgcatcgccg tttcggggtt ccaggccagc gcgcgcatga cggacgtcgc tccgcgcgtg
     2341 tcacgccgcc ggcgcggcgc tgccgacgag gccggcccgc caggcgaaat caccgaaccg
     2401 ttcgccgggc gcacgctcct gggcataccg gccaaaggca gagtcgagcg ctgacaggat
     2461 ggttggctca tcgacgttct ccaggtgcag cgcgttcagg cgctcgccgc gcccgtcgcc
     2521 gccgtagaaa aggttgtagc gtccgggcgc cttgccgacg agcgccactt ccgcgaggta
     2581 gggccgagcg cagccgttcg ggcagccggt gattcgcagc acgagcggct cgccggcgag
     2641 gccgtgggcc gcaagccggt cctcgaccag cttgacgagc cgcggcaggt agcgctcggc
     2701 ctcggccatc gcgaggccgc aggtcggcag cgcaacgcag gcgagcgcgt cgcgcgccac
     2761 cggcgaggcc tgctcgtggc ggttgaggcc gtgcttcacg accagggcgc tgatgaacgc
     2821 gcgggcactg gcatcgacgt tggcgatcgt gaggttctgg ttcggcgtca gccggaagtc
     2881 gccgcggtgc accttggcga tctcgcggag gccggtgagt tgcttcgcgc gctcggtgtc
     2941 ggcgacgcgt cccgcctcga tccgcacggt caggtgccag cggccgtcgt agccttcgat
     3001 ccagccgaag cggtcgccct gctgcgtgaa cgtgaacggc cgggccggtt ggaactcgaa
     3061 cccctgccga cgcgtgactt cggcgacgaa ccagtcgagg ccgcggtcgt cgatcgtgta
     3121 cttgagtcgt gcgcgcttgc gcacggcacg gttgccgaaa tcgcgctgcg tcgtgagcac
     3181 ggcctcggcc gtcgcgagca gctgcccggg ctcgacgaag ccgaccacgt cggcgagacg
     3241 cggataggtt tccgggtcgc cgtgcgaggc acccatgccg ccgccgatcg tcacgttgaa
     3301 accctgcagt tcgccgtctt cgacgatcgc gatgaagccc aggtcgtggg cgaagacgtc
     3361 gacgtcgttg acgggcggca cgacgatcgc ggtcttgaac ttgcgcggca ggtaggcctc
     3421 gccgtagagc gggtcggcct ccggcgtgcc gacgactttc tcgccgtcga gccagatctc
     3481 gtggtaggcg cgggtccgcg gcagcaggtg ctccgacagc ttcgtggccc actgcgccac
     3541 caccgggtgc gcgcgcgtct cgaccgggtt ggcgctcgcg agcacgttgc ggttgacgtc
     3601 gccgcaggcc gcgatggtgt cgatcagcgc ggcgttgatc gcctgcatcg tcgacttgag
     3661 ctcggtcttg atcacgccgt ggaactggaa cgcctggcgc gtcgtgatcc ggagcgtgcc
     3721 gttggcgtag cgccgcgcga tctcgtcgag ctgcaaccac tgctgcggcg tgcagactcc
     3781 gcccggcagc cgcgtgcgga tcatgaagct gtaggcaggc tcgagcttct gcttgcgccg
     3841 ctcctcgcgg atgtcacggt cgtcctgctg gtaactgccg tggaacttga tcaggaccgt
     3901 gtcgtcctcg cggattgcgc ccgtgagcga gtcgcgcagg ctctcgtcga gcgtgccgcg
     3961 cagctggcgg ctctcgcgct tgatcttctc gacgtgtgac agctcggtca tcagggttcc
     4021 acttgccggt cagtaaacgt cgcgcaggta acggcgctcg tcggcgagcg cgcgcacgta
     4081 ggcttcggcg tcctcgcggc gacggccgcc gtgctcggcg accagatcga cgagcgccgc
     4141 ctgcacgtcg ggcgccatct gctcggcatc gccgcacacg tacaggtacg cgccaccctc
     4201 gagccagcgg tagacctcct tgccggcggc gcgcagccgg tcctgcacgt aggtgcgcgg
     4261 cgtgctgcga tcgcgcgaga aagccgcgtc gagccgcgtc agcacgccct tgcggacggc
     4321 gtcctgccac tcgacctggt agaggaactc gctctggaag tgcctggcgc cgaagaacag
     4381 ccagttgcgg cccgtggcgc cctgcgcctc gcggtgctgc aggaagccgc ggaacggcgc
     4441 cacgcccgtg ccggggccga tcatgatcac gtcgcgcgag gcgtcgttcg gcagccggaa
     4501 gcgctcgttg tgctcgatga agacgcgcgc gcgcgccgat tccgccggga gccccgcaag
     4561 gtacgacgag gcggcgccaa gccggcgctg gcccgcgtgc tcgtattcga cgacggcgac
     4621 cgtcacgtgc gcttcctcgc cgaccgcctc cggactcgag gcgatcgagt acaggcgcgg
     4681 tgccagcgga cgcagggcct gcacgaagcc ccgggcgtcc cagtcgccgc ggtggcgctg
     4741 cagcacgtcg acgacctgca ggtccttgag cgtgctgcgc agcgcctgtt catggcccgg
     4801 ctgcagcacg gcggccaacg ccgggtcctt cgagcgctcg gcctgctgca cgagaaacgg
     4861 ccgcgtcagg cgcgtgatct cgcgactgcc cgtgagccac tcgcgcagcg tgcgcgcctg
     4921 gccgtcgagt tcgacgtcct gcgtcccgtc gagccgcagc gcctggagca cctcgtcgac
     4981 gacgggcgcc gggttctcgt gccagacgcc gagcgcgtcg ccgggcgcgt acgtgagccc
     5041 ggagccctcg agcgcgaact cgacgtggcg cacgtccttg gtggcgtcgc gggccgtgat
     5101 ccgctggttc gtcagcacct cggccgcgaa cggctgttcg cgcgagtgct gtggttcgcg
     5161 cgcgggcgcg cgcagccgcg tgacggtggc aacctgcggc gacgcaccaa gggtgtcgcg
     5221 ggcggtcgtc acgacccggt cgagccacgg cgtcgcgagc gtgtcgtagt cgacgtcgca
     5281 atcgacgcga tcgagcaggc gccgtgcgcc cagcgccgcg aggcgatcgt cgacctgctt
     5341 gcccgtttcg cagaacttcg gatagctcga gtcgccgagt gccagcaccg agtacgacag
     5401 ctgctcgagc ttcggcgcgc gacgcgaggc caggaactcg aggaacccgc gcgcgtcgtc
     5461 gggcgggtcg ccgtcgccct gcgtgctgac gacgacgacc agcaggcgct ccttcggcag
     5521 gtccttgagc gggtagttgc cggacgagta gacacgggcg gcgagcccgg cggcttccgc
     5581 cgagcggccg agtcgctcgg cgatgcgctt gccgttgccg gtctgcgagc cgtagacgat
     5641 cgtcaggcgt gcggcggact ccgggcgggc cgccggcgcg ggcgcaacgg cgcccgcgcg
     5701 attggcggcg cgctcgtgcg ccacgccggc cgcgaagccg gacagccact gcagcgacga
     5761 cggctcgagg ccctcgacca cgcgtagcag cagctcggac cgctcggggt ccagcggacc
     5821 cagcgggaag gccgagacga tgttggcact catggacgtc ctcgcctgcg cgttcggggc
     5881 agggaggcat cctagggagc gacacccgtc ggccggaaag gccgggtggt tatcggctca
     5941 taacgtcggt tatgagccgg tcaggcgggg aagaattcga tcggtttcgt ggtggtcatc
     6001 acggccacgt cgcgcgcctc gaaccggaac agcttcacac gcgccacgag tttgcgctcg
     6061 agttcggcat cgccgaggtc gctggacacc accctgcact ccgtgacgtc gccggacggt
     6121 gcgatggtca cctccagcac caccttgccc tgcagcgcgg gattctcccg gagcgcacgg
     6181 ctgtagagcg cgtagatggc ggacttgttc ttgtcgaata tgagctcgat ctcctcgcgc
     6241 gtccgcgcgg ccttggcgct gcggccggaa cgctgcgcct ccggcgccga ctcggcggcg
     6301 gtcgcagtgc ccgaggcgac cgtggtggtc gagacgccct tcaacccgac ggcgccgccg
     6361 ccgaagcctc ggctcgccgc gccgacggcg attccgccac tgccggcgtt cgcgcgcgcc
     6421 gtcaggatcg agcggtcgac gctcgagcgc tgcgtcgcgc cggtgtcgat ggcctgcgcc
     6481 gggacgtcct tcgcgaggtc gaggttgcgc agttccgcga gctggtccgc catcgccacc
     6541 aggccggacg ctgccgcttt cctgcgtgga tcccgtgccg gctcgctgcg ggcgagcggc
     6601 ttcgcagtgg gcgtcttgac ctcgggccgg gacgcatcga tcggttcggg gaccggcacc
     6661 tcgatcttcg gttgcggctg cggttcagga gcaggcctgg gcttgggctg ctcgaggatg
     6721 aactccagca cccgctccgg gaccgccggc gccacggcct gcgacggcgg cgtgaccggc
     6781 aggaacggga tgacgattcc gaagccgacg aacaggccca gcgccgcggc aagcatgcgc
     6841 cggaagcggc gctcctcggg aaagttgcgc gtccacggca ggtcgaagtc gcggaaatgc
     6901 aaggcaacgg tgctcacgga taaaacctac gctccggtgg cgccacgtcg gtacgcctgc
     6961 tcgcgctcga cgaccacgaa cgagactttc gtgtagtcgg ccgccgagct gctcgcgacg
     7021 atcctgcgca acacggcgta cggcagggac ttctccgcca tcacggtcac ttcgcggtga
     7081 gcttcggagc ccggcagcac gcccgcggat tgcgagccga gggccgcccg cagcggttcg
     7141 ttgacgcgac cgttcgtggc gcccagttcc gcgacggtca cgaccgcctc gccgttcacg
     7201 tagagcatgt cgcgcgtgac catgacgacc gtcgcctcgc gaggcttctg ctcggagctc
     7261 gactgcggga tctggatgtt cttcgagttc ggcaggatgt ccatgtcggc cgcgtgcacc
     7321 agcaggtaca cgaccaggat cgtgagcatg tcgatcatcg ggatcagcgc gagcggcccg
     7381 tgtccgccgc ctgcaccgcc cttgcctcga cgcgtgtggc gcctcagtcc gacgcggatc
     7441 atgtcggtgc gtctccgagc gcgacctggg ggaacaggga ctcgcgcacg atccgcgcgc
     7501 cgtcgacgac ctggcgcacg cgcgtggcgt ccatgacctg cacgatggtg tcgtagtcca
     7561 cgtcgtcggc cacgagcagc gtcgcgctgg tcacctcggg cacccgctgc ttgagcccgg
     7621 cgaggtattc ggtcagggcc gcgacgtcct ggccggtcgc ggcggccgga atcgtcgcca
     7681 gcacgcccgt gcttcgctcg gccacgtcga tgccggagcg tcgcaggatc aactcgagtt
     7741 cgaagctcgg cggagtcgcg gcggcctgcg ttcccggcac gggcaggttc acctcgagca
     7801 cgctggtctt cgagaacacg gcggtgaaga tcaggaagaa cgtcaggatc accatcacgt
     7861 ccagcatcgg aacgatgttg ataccgcggc cgcggtagcg ggcgtgattg cgctgctggc
     7921 gacgggccag cgcggagccg cgtctcatgc gcgtgccgcc tcgcgcagca cgtcgtcggg
     7981 cacgcgaccg cccttgcgtt gcgccgcggc cgccgtcgct ccgcctgcca ccgactggga
     8041 gtaggcctgc aggcccgagt cacgcgcctc gaaaaccgtg ttcaggaact tgatcgaggc
     8101 cacctcgagg ctgtcgacca ggttggtcgt gcgcatctcg aggtacatgt ggcacagcag
     8161 cagcgtgatc gcgagaccga ggccgagggc ggtgttgttc atcgcgacgg agatgcttgc
     8221 cgagagcatg ctcgccttct cggccgggtt gacggccgcc accgccgcga acgcgtggat
     8281 caggccgatc acggtgccga gcaggcccat gagcatgccg acgttcgcga gtcccgcgag
     8341 ggcctgggtg cgcttttcga ggcgcgggat gacctcgatg aggctttcct ccatcgcctt
     8401 ctcgacgtct tcgcggcgct gcgcgttcgc ggcccgcgac agcccgtagc gcatgaccga
     8461 ggacagcgcc gagtcggtct gttcggccag ctccagcgcc ttgcggaaat tgccctgctg
     8521 cagcagcggc acgagcttcg cccacaggcg acggttgccg cgcgagacgc gcgtcaggta
     8581 gatgaagcgt tcgacggcca ggaccaggcc gatcacgaac acgccggcaa tcgggtagag
     8641 gaagggtcca ccctgcttga agaagttgac gatcaggtcg acggtactca tggctgcgac
     8701 actccgggga cttcaacgtg aggggcgaca ggggcggtcc gcgacgtcag gtcgcggtgg
     8761 taggcgatct cgcgccggaa ctcgtctcga tcgagcggcg tcagcagttc ctcgatcagg
     8821 ctgcccatgg gccgatccgg accggcgggc ggctcggcgg ctttccaggg aacgatgctc
     8881 atgaccttcg gcagctcgcg gttgccggtg atcgaggtcg cgtcgaggtg caggcgatcg
     8941 accgcgcgtt ccccggactt cgccgtcgcg ctgacgacag gctcgacggg aggttcggac
     9001 gcccgaaccg gcgaggcgca aacggcgatg gccgccaggg acgccagcca gcggcacgta
     9061 ttccgggctc gcgccgcggg aagcggctcg cctgtgactg gccgtaacgg gtcgagcatc
     9121 gtcatggcgt ggccacttcc gcagtacgtg tgacggtgga gaggcgcgtc ctgagttcca
     9181 cgagccaggc tgcgacctgc acgtcggctc cctgcttgag cgcctggtaa cgctcgtagt
     9241 gaggcaacgc agcgacgggg tcgtccaggt acaggtcgtg caggatgccg aggttgcgtt
     9301 cggcgtcggc gtagccggga tcgagggcga gcgcccgctc gtaggcttcg cgcgcctcct
     9361 cgaaggcccc ggcgcggcgg cgcgcgacgg caagctggtt cagcgctgcc ggatcggact
     9421 ccggcgccac gacgtcctcc gccggcgcag cgacgacttc gtcacgcgca tagcgcgcgg
     9481 gctcgagctg cgcgagatcg acgtagctcc tctgcaccca ttcgtcgtag atgccctgtg
     9541 ccgcgtgacg cgcattgcgt tcgtgcaggg cgatggcctt ctcctcgaac gggaaggcct
     9601 gttcctcgag caggacctcg tactgttcga gctcgtcgtc gtcgaggtca tcgggccggt
     9661 ccgactcgag caacgagcgg ccgagttcgc gatacaggtc agccatggca taagcggccg
     9721 cggtcgtgac ttgcgcgatc ccgtagcttt cggcccggcc gtaggtggcg agcgccgcct
     9781 ggagggcctc gcgcttggcc gccagcgacc ggtcgagcgg cagcgccagc cgaatcgcgc
     9841 gcgcctgcgc gtcgagcggc cgggcgagct ccagtgtcgc cgtggcagcc agcagccggg
     9901 tccggtcgct gcgttcggca ccggcgactg cgtcggcggc gacgagcgct tccagccagc
     9961 gccgtcggcc caggccgtcg ttcgcgtcgc gtgcgtaatc ggccagcgtc tggcgggcct
    10021 cgatcgccgg cgcggccggc accgggaacc gctcgacgta gacgccgtac gcgcgcgtcg
    10081 cgccgactcg atcgcccgca ccggcgtaca gctcggccgc ctgccactgg gcggcgcggc
    10141 gcacctccgc ctcttcgtcc ggacgcgcgg ccacccgctc gtactccgca gcggcgcgcg
    10201 tggactcccc ggattcgacg taggcgacgg cgagcctggc ctcgacctgg cgcgcgaggg
    10261 cgtggcccgg atgatcgcgc cggaactgct cgaagacctg cgcggcctga ggccagcgcc
    10321 tggcttcgag cagcagcgtg gccgcgtcga attcagcgtt ggcccggatc ggtgacgcgg
    10381 cggccacgac gccgatgcgc aggaagtcct gcacggcgcc gtcgacgtcg cccgcggcac
    10441 ggcggacctc ggcctgccga tagatcgagg ccgcgcgacg ctccaccgac tccgcgtact
    10501 gcggatcgtc ccgcgacagc cgtgcgacca cctcggcgta ggcgcgctcg gcctcggcga
    10561 accgggacga atcgaaatag gtgtgcgcaa gcacggtcca cgccactcgc tgctgttccg
    10621 gcgtggcgcg cggcccgagc ccaagcacgc gctgcgccag ggactcggcc cgctcgcggt
    10681 cgtccgagtc gaacacgagc ttgcacgcgc gggtcagcac cggcggtgtc tcggtgtgcg
    10741 cgggaaacgc ttccgcgaac ttcagcgacg cttcgacgac gcgctcgttc agccgcaatc
    10801 ggtccggatc ggtcatctgc agcgccgcct tgtcgagcgc gacgagcacc gcgtagccgg
    10861 cgcgcgccgc ctcggccgca ttcgggtagc cataggcggc ccggtcgtac tccaccgccg
    10921 cctccgcgaa tcgctgcccc tcgaacagca ggtccgcgag caacaggc
//
LOCUS       DASPFR010000401         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1584618, whole genome shotgun sequence.
ACCESSION   DASPFR010000401 DASPFR010000000
VERSION     DASPFR010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584618"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1609
                     /locus_tag="VD737_10705"
     CDS             <1..1609
                     /locus_tag="VD737_10705"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11079.1"
                     /translation="QAPATVNDVRTIEGPSPGGRYGSAFPLHDGTGRLLVTWTQCRLL
                     EQPTSPNPRIVPCTEEGMAMDATVAPPLYGVWIYDPSQRTQLPVLSPVEGVLVTEVVA
                     LEARAMPPVLLDRTAGVDLPAELAAEGVGIVDIGSVYDVDGNDVAPDGIVTLRHPGLT
                     LAAARPARFVRLEKAVSLPDRDVRDFDRSAFGVTDAFGMREIVGYAPVDPDGSVRIKV
                     PADVAFAITALDAQGRRIGPRHDVWLQVRAGQELKCQGCHDPREGRSHGRENLFAPVN
                     PGAPTTGSPFPGTQPAMFADFGETMAQVRGRISCQSDCAALTPDTAIRYEDVWTDPIA
                     AGRAPDTAFTYSYLDLATPAPTSADCVSGWHAGCRITIHYERHIHPLWSRSRITLAAD
                     GTVARNDTCTSCHSATAADGTTRVPASQLDLADGPSSEEPAHFKAYRELLDEDAAQEL
                     AGGQLRDRLVQEGVDPVTGQPQFATVPVEPPLVAGSAGESTRFFTLFSPGGSHAGRLA
                     PAELKLVAEWVDIGAQYWNDPFAAPAN"
     gene            1617..>2183
                     /locus_tag="VD737_10710"
     CDS             1617..>2183
                     /locus_tag="VD737_10710"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HXW11080.1"
                     /translation="MLRALPALLLALACAASAPAVATARTRATVVVKDPYLDLHTGPG
                     RGYPSTYSVERDATVELLRRRTDWIKVRTREGRAGWVHRSQLERTLTPQGDEVRLPGP
                     SPDAPLEHRWETGLATGDFGGANVVSVTGNWVANPHLLLRTDVSQLLGTYSDGWLGTI
                     GIAHVFVPQWRLSPFAGLGGGMLTISPKA"
BASE COUNT          321 a          780 c          746 g          336 t
ORIGIN      
        1 gcaggcgccg gccaccgtga acgacgtgcg cacgatcgaa ggcccctcgc cgggaggccg
       61 ctacggctcg gcgttcccgc tgcacgacgg cacgggccgg ttgctggtca cctggacgca
      121 gtgccgtctt ctggaacagc cgacctcgcc gaacccgcgg atcgtgccct gcaccgaaga
      181 aggcatggcg atggacgcga cggttgcgcc accgctctac ggcgtgtgga tctacgaccc
      241 ctcgcagcgc acgcagctcc cggtgctgag ccccgtcgag ggcgtgctgg tgaccgaggt
      301 cgtcgcgctg gaagcgcgtg cgatgccgcc cgtgctgctc gaccgcacgg caggcgtcga
      361 cttgcccgca gaacttgccg ccgaaggtgt cggcatcgtg gacatcggca gcgtgtacga
      421 cgtggacggt aacgacgtgg ctccggacgg catcgtgacg ttacgacacc cggggctcac
      481 gctcgccgcg gcgcggccgg cacggttcgt gcgcctcgaa aaggccgtca gcctgcccga
      541 ccgggacgtg cgcgacttcg accgcagcgc gttcggcgtc accgacgcgt tcggcatgcg
      601 cgaaatcgtc ggctacgcgc cggtcgaccc ggacggttcg gtacgcatca aggtcccggc
      661 cgacgtcgcg tttgccatca cggcgctcga tgcgcagggg cggcgcatcg ggcctcgcca
      721 cgacgtctgg ttgcaggtac gggccgggca ggaactgaag tgccagggct gccacgaccc
      781 ccgcgagggt cggtcgcacg gccgcgaaaa cctgttcgcg cccgtcaatc ccggcgctcc
      841 gacgacgggt tcgccctttc cggggaccca accggcgatg ttcgcggatt tcggcgagac
      901 catggctcag gtccgcggcc ggatcagttg ccagagtgac tgcgcggcgc tcacgcccga
      961 cacggcgatc cgctacgagg acgtctggac ggacccgatc gctgccggtc gggcgccgga
     1021 caccgcgttc acctacagct atctcgacct ggcgacaccc gctccgacct ccgccgactg
     1081 cgtctccggc tggcacgccg gctgcaggat cacgattcac tacgagcggc acatccatcc
     1141 gctctggtca cgatcgcgca tcacgctggc cgccgacggc acggtcgccc gcaacgacac
     1201 ctgcacgtcc tgtcattccg ctaccgcggc cgacggcacg acgcgcgtgc ccgcgtcgca
     1261 gctcgacctg gccgacggac cgtcgagcga ggagcccgcg catttcaagg cctatcgcga
     1321 gctgctggac gaagacgccg cacaggaact cgcgggtggc cagctgcgcg atcgactcgt
     1381 gcaggaaggc gtcgatccgg tcacgggcca gccccagttt gcaacggtgc cggtggagcc
     1441 accgctcgtc gccggcagtg ccggcgaatc cacgcgtttc ttcacacttt tctcgcccgg
     1501 cggctcgcac gcgggtcgcc tggcacccgc agagttgaag ctcgtcgcag aatgggtcga
     1561 catcggcgcg cagtattgga acgacccgtt tgctgccccg gccaattgac gacccgatgt
     1621 tgcgcgctct gcctgccctg ctcctggcgc tcgcctgcgc cgcgtcggcg cctgccgtcg
     1681 cgacggcgcg gacgcgcgcg acggtcgtgg tcaaggaccc gtacctggac cttcacaccg
     1741 gtccgggccg cggctatccg tcgacgtact cggtcgaacg cgacgccacg gtcgagttgc
     1801 tgcgccggcg caccgactgg atcaaggtgc gcacgcgcga gggacgcgcg ggctgggtcc
     1861 accgatcgca actcgagcgc acgctgacgc cgcaaggcga cgaggtccgg ctgccggggc
     1921 cttcgcctga cgcgccgctg gagcatcgct gggagaccgg gctggcgacc ggcgattttg
     1981 gtggcgcaaa cgtcgtttcg gtcactggca actgggtggc gaatccccac ctgctgctgc
     2041 gcaccgacgt gagccagttg cttggcacgt attcggacgg ctggctcggc acgatcggca
     2101 ttgcgcacgt cttcgtaccg cagtggcgcc tttcgccatt cgctgggctc ggcgggggca
     2161 tgttgacgat ctcgccgaag gcc
//
LOCUS       DASPFR010000402         5236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1219873, whole genome shotgun sequence.
ACCESSION   DASPFR010000402 DASPFR010000000
VERSION     DASPFR010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219873"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /gene="rpoA"
                     /locus_tag="VD737_10715"
     CDS             complement(<1..783)
                     /gene="rpoA"
                     /locus_tag="VD737_10715"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HXW11081.1"
                     /translation="MPQSGFLKPRIVDVQNLSPNHARVTMEPFERGYGHTLGNALRRV
                     LLSSMPGYAPTEVQIAGVVHEYSTLDGVREDVVDILLNLKGVVFRLHNRTEAILTLKK
                     RGGEGNGAVTAADIEPAHDVEIVNPDHVIAHLAAGGKLEMQIKVESGRGYVPGNMRYL
                     PEETKGIGRVILDASFSPVRRVSYAVESARVEQRTDLDKLIMDIETNGSIEPEEAIRY
                     AARVLVDQLSVFAQLEGTSLPTEAPKSPTVDPILLRPVDDLEL"
     gene            complement(797..1423)
                     /gene="rpsD"
                     /locus_tag="VD737_10720"
     CDS             complement(797..1423)
                     /gene="rpsD"
                     /locus_tag="VD737_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HXW11082.1"
                     /translation="MARNLDKCRHCRREGEKLFLKGEKCFTDKCPVERRAYAPGQHGQ
                     KSGARLSDYGKQLREKQKVRHIYGLLERQFRKTYAEAARSKGVTGERLLQLLESRLDT
                     VVYRMGFGASRTEARQIVRHNGILVNGKRVNIPSYQVRPGDAIEVAGKAKEQLRIKAA
                     AEAAASRSIPEWMEVDSKGLKGTFKALPARSDLPQTINESLIIELYSK"
     gene            complement(1436..1861)
                     /gene="rpsK"
                     /locus_tag="VD737_10725"
     CDS             complement(1436..1861)
                     /gene="rpsK"
                     /locus_tag="VD737_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009514913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HXW11083.1"
                     /translation="MASRNQPTRQELQQTASARARKKVKKNVAEGIAHIHASFNNTIV
                     TITDRQGNALAWATSGHAGFKGSRKSTPFAAQVAAERAGRAAQECGVKNIEVRIKGPG
                     PGRESAVRALNAIGLKIGSIADVTPIPHNGCRPPKRRRV"
     gene            complement(1865..2248)
                     /gene="rpsM"
                     /locus_tag="VD737_10730"
     CDS             complement(1865..2248)
                     /gene="rpsM"
                     /locus_tag="VD737_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HXW11084.1"
                     /translation="MARIAGINIPNHQHAEIALTAIYGIGRARAQAICQAAGVQGNRK
                     IKDLHDAELERLRENVQKLSVEGDLRREVQMSIKRLIDLSCYRGSRHRKGLPVRGQRT
                     RTNARTRKGPRKAAIKLNVPAGKAA"
     gene            complement(2287..2400)
                     /gene="rpmJ"
                     /locus_tag="VD737_10735"
     CDS             complement(2287..2400)
                     /gene="rpmJ"
                     /locus_tag="VD737_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HXW11085.1"
                     /translation="MKVMASVKRICRKCKIVRRKGVVRVICSDPRHKQRQG"
     gene            complement(2408..2626)
                     /gene="infA"
                     /locus_tag="VD737_10740"
     CDS             complement(2408..2626)
                     /gene="infA"
                     /locus_tag="VD737_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HXW11086.1"
                     /translation="MPKEDVIELQGEVLENLPNATFRVKLENGHTIHAFISGKMRMNY
                     IRILPGDKVTVQMTPYDLTKGRITFRAK"
     gene            complement(2637..3959)
                     /gene="secY"
                     /locus_tag="VD737_10745"
     CDS             complement(2637..3959)
                     /gene="secY"
                     /locus_tag="VD737_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HXW11087.1"
                     /translation="MATALPNVGKASRYGDLKRRLLFLLAALVVFRIGAHIPCPGIDP
                     NELAKLFEGQQGGILGMFNMFSGGALSRFTIFALGIMPYISASIIMQLMTAVSPQLDQ
                     LRKEGEAGRRKITQYTRYGTVFLALFQATGISIALESQPGLVLDPGLMFRITTVTSLV
                     TGTMFLMWLGEQITERGIGNGISIIIFAGIAAGLPQAIAGTLELVRTGAMHPLTALLI
                     AVAVLVVTAFVCFVERGQRKILVNYAKRQVGNRVYGGQSSHLPFKLNMSGVIPPIFAS
                     SLILFPATLLGWFGSAEGMLWLRDIAGTLTPGQPIYVITYGALIVFFCFFYTALQYNP
                     KETADNLKKSGAFVPGIRPGDQTARYLEKILTRLTLAGAVYVTAVCLLPEFLILRWNV
                     PFYFGGTSLLIIVVVTMDFMAQVQAYVMTHQYESLLRKANFKGGLPVR"
     gene            complement(4043..4474)
                     /gene="rplO"
                     /locus_tag="VD737_10750"
                     /pseudo
     CDS             complement(4043..4474)
                     /gene="rplO"
                     /locus_tag="VD737_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            complement(4464..4643)
                     /gene="rpmD"
                     /locus_tag="VD737_10755"
     CDS             complement(4464..4643)
                     /gene="rpmD"
                     /locus_tag="VD737_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HXW11088.1"
                     /translation="MKKIKVTLLKSVAGIRRTHRATVRGLGLKRINHVVELADTPAVR
                     GMINKVAYLVRIDAA"
     gene            complement(4640..5158)
                     /gene="rpsE"
                     /locus_tag="VD737_10760"
     CDS             complement(4640..5158)
                     /gene="rpsE"
                     /locus_tag="VD737_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019519200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HXW11089.1"
                     /translation="MAKFQPRAQTEGNDDGLKEKMIAVNRVTKVVKGGRILGFAALTV
                     VGDGDGRVGMGKGKAREVPVAVQKAMEEARRKLFKVKLKNGTLHHVVTGRHGAARVLM
                     QPAHEGTGIIAGGPMRAVFEVMGVTNVLAKCFGSTNPYNVVRATLNGLRSMSTPAEIA
                     ARRGKKVEEISA"
BASE COUNT          753 a         1725 c         1749 g         1009 t
ORIGIN      
        1 gagctcgagg tcgtccaccg gccgcagcag gatcgggtcg acggtgggcg acttgggcgc
       61 ctcggtgggc agcgaggtgc cttccagctg ggcgaacacc gacagctggt cgaccaggac
      121 gcgggccgcg tagcggatcg cttcctccgg ctcgatcgag ccgttggtct cgatgtccat
      181 gatcagcttg tcgaggtccg tgcgctgctc gacgcgcgcc gactccaccg cgtaggacac
      241 gcggcgcacc gggctgaagg aggcgtcgag gatgacacgg ccgatgccct tggtctcctc
      301 gggcaggtag cgcatgttgc cggggacgta gccgcggccc gactcgacct tgatctgcat
      361 ctcgagcttg ccgcccgcgg cgaggtgcgc gatcacgtgg tcggggttga cgatctcgac
      421 gtcgtgcgcg ggctcgatgt cggccgcggt caccgcgccg ttgccctcgc cgccgcgctt
      481 cttgagcgtc aggatcgcct cggtgcggtt gtgcaggcgg aacaccacgc ccttgaggtt
      541 gagcaggatg tcgaccacgt cctcgcgcac gccgtcgagc gtcgagtact cgtgcaccac
      601 gccggcgatc tgcacttcgg tcggcgcgta gcccggcatc gacgacagca gcacgcggcg
      661 cagcgcgttg ccgagcgtgt ggccgtagcc gcgctcgaac ggctccatgg tgacgcgcgc
      721 gtggttcggc gacaggttct gaacgtcgac aatacggggc ttcaggaatc cagattgcgg
      781 catgtagggt ccttctttac ttcgagtaca gctcgatgat caggctttcg tttatcgtct
      841 ggggcaggtc gctgcgcgcg ggcagcgcct tgaacgtgcc cttcagcccc ttcgaatcca
      901 cttccatcca ttccgggatg ctgcgcgagg ccgcggcttc ggctgcggcc ttgatgcgca
      961 gctgctcctt cgccttgccg gcgacctcga tcgcgtcgcc gggccgcacc tggtacgacg
     1021 ggatgttgac ccgcttgccg ttcaccagga tgccgttgtg gcgcacgatc tggcgcgcct
     1081 cggtacgcga ggcgccgaag cccatgcggt acaccaccgt gtcgaggcgc gactcgagca
     1141 gctgcagcag ccgctcgccg gtcacgccct tggagcgcgc cgcctcggcg taggtcttgc
     1201 ggaactggcg ctcgagcagg ccgtagatgt ggcgcacctt ctgcttctcg cgcagctgct
     1261 tgccgtagtc ggaaagccgc gcgccgctct tctggccgtg ctgcccgggc gcgtaggcgc
     1321 ggcgctcgac cgggcacttg tcggtaaagc acttctcgcc cttcaggaag agcttttcgc
     1381 cttctcgccg gcaatgccgg cacttgtcga ggtttctagc cattagatct ggggattaga
     1441 cccggcgtcg cttggggggc cggcagccgt tatggggaat cggcgtcacg tcggcgatgc
     1501 tgccgatctt gaggccgatc gcgttcagcg cgcgcaccgc cgactcgcgg ccgggcccgg
     1561 ggcccttgat gcgcacctcg atgttcttca cgccgcactc ctgcgccgcg cggccggcgc
     1621 gctccgcggc cacctgcgcg gcgaacggcg tcgacttgcg cgagcccttg aagccggcgt
     1681 ggcccgaggt ggcccaggcg agcgcgttgc cctggcggtc ggtgatcgtg acgatggtgt
     1741 tgttgaacga cgcgtggatg tgcgcgatgc cctcggcgac gttcttcttg accttcttgc
     1801 gcgcgcgggc ggaggcggtt tgctgcagct cctggcgcgt cggttggttt ctgctggcca
     1861 ttgattaagc agcctttcct gctggaacgt tgagcttgat tgcggccttc ctcgggccct
     1921 tgcgggtgcg cgcgttggtg cgcgtgcgct ggccgcgcac cggcagcccc ttgcggtggc
     1981 gcgagccgcg gtagcagctg aggtcgatga gccgcttgat cgacatctgc acctcgcggc
     2041 gcaggtcgcc ctcgacgctg agcttctgca cgttctcgcg cagccgctcg agctcggcgt
     2101 cgtgcaggtc cttgatcttg cggttgccct gcaccccggc ggcctggcag atggcctgtg
     2161 cgcgggcgcg gccgatgccg tagatcgcgg tgagcgcgat ctcggcgtgc tggtggttcg
     2221 ggatgttgat tcctgcgatt cgggccatag agaacctgaa attatatctg gttgaacaag
     2281 gcgttatcaa ccttgccgct gtttgtgcct ggggtcggag cagatgaccc gcacgacccc
     2341 cttgcgccgc acgatcttgc atttgcggca gatcctcttc actgatgcca tgactttcat
     2401 aaatgcctca tttggccctg aatgtgatgc ggcccttggt caggtcgtag ggcgtcatct
     2461 gcacggtcac cttgtcgccc ggcaggatgc ggatgtagtt catgcgcatc ttcccggaga
     2521 tgaaggcgtg gatggtgtgg ccgttctcga gcttcacgcg gaaggtcgcg ttcggcaggt
     2581 tctccagcac ctcgccctgc aactcgatga cgtcttcctt cggcatcggc gcgcatttac
     2641 ctgacgggca ggcctccctt gaagttcgcc ttgcgcagca ggctctcgta ctggtgcgtc
     2701 atgacgtacg cctgcacctg cgccatgaaa tccatcgtca ccacgacgat gatgagcagc
     2761 gacgtgccgc cgaagtagaa cggcacgttc cagcgcagga tcaggaactc gggcagcagg
     2821 cacaccgccg tcacgtagac agcgccggcg agggtcaacc gagtgagaat tttttcgaga
     2881 taccgcgcgg tctggtcgcc gggccggatg ccggggacga aggcgcccga cttcttcagg
     2941 ttgtcggcgg tctccttcgg gttgtactgc agcgccgtgt agaagaagca gaagaatacg
     3001 atcagcgcgc cgtacgtgat gacgtagatc ggctgcccgg gcgtgagcgt gccggcgatg
     3061 tcccgcagcc acagcattcc ctcggccgag ccgaaccagc cgagcagcgt cgccgggaaa
     3121 aggatcagcg agctcgcgaa gatcggcggg atcacgcccg acatgttgag cttgaacggc
     3181 aggtgcgagc tctggccgcc gtagacccgg ttgccgacct ggcgtttggc gtagttcacc
     3241 aggatcttgc gctggccgcg ctcgacgaag cacacgaaag cggtgacgac caggaccgcg
     3301 accgcgatca gcagcgccgt gagcgggtgc atcgcgccgg tgcgcacgag ctcgagcgtg
     3361 ccggcgatgg cctgcggcag cccggcggcg atgccggcga agatgatgat cgagatgccg
     3421 ttgccgatgc cgcgctcggt gatctgctcg ccgagccaca tcaggaacat ggtgccggtg
     3481 acgagcgagg tcaccgtggt gatgcggaac atgagccccg ggtcgagcac cagccccggc
     3541 tgtgattcga gcgcgatcga gatcccggtc gcctggaaga gggccaggaa caccgtgccg
     3601 tagcgcgtgt actgcgtgat cttgcgccgg cccgcctcgc cttccttcct cagctggtcg
     3661 agctgcgggc tgaccgcggt catcagctgc atgatgatcg aggccgagat gtacggcatg
     3721 atgccgagcg cgaagatcgt gaagcgcgac agcgcccccc cggagaacat gttgaacatg
     3781 ccgaggatcc cgccctgctg cccctcgaac agcttggcca gctcgttcgg atcgatgccc
     3841 gggcacggaa tgtgcgcgcc gatgcggaac acgaccagcg ccgcgagcag gaacagcagc
     3901 cgccgcttca ggtcgccgta cctgctcgcc ttgccgacgt tgggtagcgc ggttgccatc
     3961 aggacttgga cttcggctca gcctccggct cggccttctt ctgcttcttt accttttttt
     4021 cctttaccgg cttctcgatg acttcgatcg tcccgcccgc ggcttcgatc gcggcgcgcg
     4081 cgcccttgga ggcccggacg tcgcccttca cgttgagctt cttcgtcagc ttgcccgcca
     4141 cgatgatctt cgcgccgagc gccttcctcg acacgatgcc cgcggcctgc agcgcggcga
     4201 gatccacgtc ggccccctga agcgtctcga gctccgagag gcgcacttcg gcgatatcgt
     4261 ctcgggtggg cgagttgaag ccgcgcttcg gcaggcggcg gtgcagcggc atctggccgc
     4321 cctcgaagcc gagcttgtgg aagccgcccg agcgcgcctt ctgccccttg tggccgcgcc
     4381 cggcggtctt gccccagccc gagccgacgc cgcggccgac gcggtgacgg tccttggtgg
     4441 cgccgggcgc cggtttcagt gtgttaagcc gcatcgattc gcaccaggta ggcaaccttg
     4501 ttgatcatgc cgcgcaccgc cggagtatcg gcgagctcga cgacgtggtt gattctcttg
     4561 aggcccagcc cccgcacggt ggcgcggtgg gtccgcctga taccggcgac gcttttcagc
     4621 agcgtgacct tgatcttctt catgcgctga tttcctcgac cttcttgccc cgccgcgccg
     4681 cgatctcggc gggcgtgctc atcgagcgca ggccgttcag cgtcgcgcgc accacgttgt
     4741 aggggttggt ggagccgaag cacttggcga gcacgttggt gacgcccatc acctcgaaca
     4801 ccgcgcgcat cgggccgccc gcgatgatgc cggtgccttc gtgcgcgggc tgcatcagca
     4861 cccgggccgc gccgtggcga ccggtaacga cgtgatgcag cgtgccgttc ttcagcttca
     4921 ccttgaacag cttgcggcgc gcttcctcca tcgccttttg caccgcgacc ggcacttcac
     4981 gcgccttgcc cttgcccatg cccacgcggc catcgccgtc accgacgacg gtgagtgcgg
     5041 cgaaaccgag gatacgacca cccttgacca ccttggtcac gcggttcacc gcaatcatct
     5101 tttccttcag gccgtcgtcg ttgccttcgg tctgggcgcg gggttgaaac tttgccattt
     5161 gtgatcctta gaactgcagg ccggcttcgc gggctgcctc ggccagggcc ttgacgcggc
     5221 catggaacgc aaagcc
//
LOCUS       DASPFR010000403        10569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_853877, whole genome shotgun sequence.
ACCESSION   DASPFR010000403 DASPFR010000000
VERSION     DASPFR010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10569
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853877"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VD737_10765"
     CDS             complement(<1..688)
                     /locus_tag="VD737_10765"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HXW11090.1"
                     /translation="MNDASTLTQRAAHDPRLTSLRRWLERDLGFSDYRLAPASADASF
                     RRYFRVTRAGHEPLIVMDAPPGKEDVGPYVRVATLLQECGVNAPRVLERSPGDGFLLL
                     TDLGTTMYLPELALPGRADPLYADAIAALVRIQARGAEAARTLPPYDEKLLRFEMSLF
                     PDWLLQRHLQLELSDHESRVLDAAMDALVANALEQPQVFVHRDYHSRNLMVCPGHNPG
                     ILDFQDAVRGG"
     gene            823..3096
                     /locus_tag="VD737_10770"
     CDS             823..3096
                     /locus_tag="VD737_10770"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HXW11091.1"
                     /translation="MRSNRGQRTVGTGAVFGLRTTLLAACLATSVPRVLAADAPADEP
                     ATSDACRVATPDPRVEELLQADPGDPRIDITSDTGELGRAGDAVLSGNVTIRTGQRLL
                     KADQAEVDAERRSVRLRGRMEYLDPQLHVVGDSGSFIEGGGGQFKGAQFELVERSVRG
                     AAEGARVTPDGKMELEGVRYTACPPGVDDWELQAGEIAIDQKTQIGTGRDVKLEFLGL
                     PIFYTPWITFPVGDQRKSGLLFPTIGSSSKTGTLVAVPYYWNLAQNYDATLTSRWYSS
                     RGFRVDPELRYLDDRTRSQLNVEYMFHDEDRGESRSLVDWRHVTRLAPRTRLLIDAAH
                     VSDTDYFEDFGVGFEGTSVTYLDRYVDLRHDTGPWSFTARAQGHQVIDTELPEEDEPY
                     QIVPQLTANGRWRDLPGGLGASLYAEATNFARPLGPEGARFDAAPSLEWRIDRGGGFF
                     AANAGYRYTQYALQDVAPGVDDSPSRSLPTASLDTGLVLERTAGSRGNRLQTVEPRLL
                     YLHVPYRDQDDLPVFDTGLPDLNLVQLFRDNRYVGPDRVGDANQVSVGVTTRLLDSTR
                     GTQYLSATLGQAYYFEDPRVRLPDEPVRDRSSSDVVAEIELAAFRNWNARFAYQWNPD
                     QSQGERSETFIQYAPAPGRVLNAGYRFRRDLLEQVDVSGAWPINARWRGFARYVYSLQ
                     EEKTLDQFVGFEYSSCCWAVRLITRRFVSSRSGDAETSFGLQLELKGLSSVGVDNEAF
                     LREAIRGYSAIPSAPRT"
     gene            3104..4417
                     /locus_tag="VD737_10775"
     CDS             3104..4417
                     /locus_tag="VD737_10775"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXW11092.1"
                     /translation="MLLKLKQFFGVLALLAAAAGTAHAQSKDLSSTGVLLDRVAAVVN
                     DGIVLRSDVESQMQAISERIQQQGQQLPPRNVLRQQVLERLVLQELQMQRAERLGIRI
                     PDEAINQTLTEVAQRNNIKFSDLPTALESQGVDYRDYRDEVRRELTMQTLRQRDVLAR
                     VYVSPREVEQCIAKRKASPNVDDEFNLSHILVAVPSTADERQIAERTSRAQAVHDRAK
                     QNEDFAQLAITYSDSGTALEGGALGWRKASQLPSFVADIIPNMQAGDVTEPIRTPSGL
                     HIFKVLEVRGGQAPAVVSQVHARHILMKPNEVEDDETVRQKLTQIRNRVLGGESFEAI
                     ASVTSEDPGSAAQGGDLGWSGRGSFVPEFERQLDALEENQISEPFRTEYGWHIVQLLG
                     RRNYDASGDVTRNRCVAQLREARADEETEIWLRRLRDEAFVEYRM"
     gene            4453..5448
                     /gene="pdxA"
                     /locus_tag="VD737_10780"
     CDS             4453..5448
                     /gene="pdxA"
                     /locus_tag="VD737_10780"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HXW11093.1"
                     /translation="MTLRTVAVTTGEPAGIGPDICGTLIEDPPADSRVVLIGDGSLIG
                     RSRLKRAHKPRLALFDATAEPRPGIELLHVPLAAPCVPGRPDPANARYVLETLDAAIG
                     GCAAGAFHAIVTAPVQKSVINEAGIPFTGHTEYLARQTGVRLPVMMLVGAGMRVALVT
                     THLALRKVSAAITPARLESVLRIVESDLRTMFGVRRPRLLVLGLNPHAGEGGHLGREE
                     IETIEPVLATLRAAGLDLAGPVPADTAFVPGRLAGFDAVVAMFHDQGLPVLKHASFGH
                     AVNVTLGLPIVRTSVDHGTALDLAGTRKADTGSLRAAFDLALDLSSRVRAQTSHW"
     gene            5445..6272
                     /gene="rsmA"
                     /locus_tag="VD737_10785"
     CDS             5445..6272
                     /gene="rsmA"
                     /locus_tag="VD737_10785"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HXW11094.1"
                     /translation="MRSRAPYAKKRYGQHFLHDPGTIDRIVRAVDPRPGDRIVEVGPG
                     RGAITAPVLDRVGALDVVEIDPDVLPLLQAHCAGRGELRVHLADALEFDFRALRGAGA
                     RLRLIGNLPYNVSTPLLFRFIGQLDAFADMHFMLQREVVARMAARPDTGDYGRLTVTL
                     APHVAVEPLFDIGTGAFKPAPQVVSTFFALRPHASPPFDTGEPAAYARVVKAAFSKRR
                     KTLRNALAGLLTPEQILAAGVDPAVRAETLPPERFGALARQCRPSTAPADSGAQGPS"
     gene            6389..6826
                     /locus_tag="VD737_10790"
     CDS             6389..6826
                     /locus_tag="VD737_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXW11095.1"
                     /translation="MREYKKILACVDLTDGSEQIAERARDMAARCGGTVTLLHVVEYV
                     PVEPMGEALLPAVQIEGELVDRATRRIADLAARAGLDTSERVVRAGNIKSEVVRIAQE
                     IGADLIVLGSRERHGVSILFNQTEDTVLHAAPCDVLAVRWNRK"
     gene            6823..7206
                     /gene="apaG"
                     /locus_tag="VD737_10795"
     CDS             6823..7206
                     /gene="apaG"
                     /locus_tag="VD737_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HXW11096.1"
                     /translation="MTETASHRIRVDVATSYVEDQSNPGEARYVFSYTITIRNEGDKP
                     ARLMGRHWLITDANGKVQEVVGEGVVGEQPHLQPGQGFRYSSGAILETPVGSMQGRYR
                     MLADDGEQFDAPIAPFRLAMPGVLH"
     gene            7216..8058
                     /locus_tag="VD737_10800"
     CDS             7216..8058
                     /locus_tag="VD737_10800"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001204.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HXW11097.1"
                     /translation="MAVYAIGDVQGCADQLEALLVRLDFDVHLDRLWFVGDLVNRGPR
                     SLDTLRLVMALGEAATVVLGNHDLHLLAIARGGAPWKRTDGGLEQVMAAPDREALLDW
                     LQAKPLMHHDAGLQASLLHAGLPPQWDVATALDCAREVERGLQGERVGELFAHMYGNE
                     PSLWHDDLEGWDRLRFTINAFTRLRVCQVDDGRMVLSHKGPPGTAPRGAVPWFRVPWR
                     RSAGERLVFGHWSALGYVDEQGVLGLDTGCVWGGALTAQRIDAAASRPVQVTNSADGL
                     PLGD"
     gene            complement(8062..8790)
                     /locus_tag="VD737_10805"
     CDS             complement(8062..8790)
                     /locus_tag="VD737_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11098.1"
                     /translation="MNGSITAAPLTAVFAALGLGCGAVPAQPASEAPAATATPPVNAV
                     WVERKQSFTYFGDTTYYSCDGLRDKVRYILKQLGARDLEVSSSCVEMGGYGVEAMPGV
                     RIKAYFAREATPELLQQIADGAAERELIARVRGRGDAADDAAAQFPAAWQRVELEGVR
                     HGRIANGDCELLQQLARQVLEPAGIRVLRQDSQLGCVRHGVPIRAVRLVLETLQPLPP
                     PDESPTTPEPTPAAGESSPATTDS"
     gene            8814..9323
                     /locus_tag="VD737_10810"
     CDS             8814..9323
                     /locus_tag="VD737_10810"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF009763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HXW11099.1"
                     /translation="MRVLAPLNLRQWIDAHRGALRPPVCNKQVFDEGEFIVMVVGGPN
                     ARKDYHDDPGDEFFYQLEGDMLLRTIQDGRRVDVPIRAGEVLLLPAHVPHSPQRFADT
                     VGLVVERRRRPGEADGFLWFCERCDHPLHSEYLQVTDIEAQLPPVFERFWSNPERTAC
                     DRCGHVMHR"
     gene            9328..10167
                     /locus_tag="VD737_10815"
     CDS             9328..10167
                     /locus_tag="VD737_10815"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXW11100.1"
                     /translation="MNALEFSTRGRHWRADLRRPIDLAIPLDFAGAQPRFFASVPASA
                     EPLRAGSFTGSVGSGASCNCATLTLAPHCHGTHTECVGHVTDPVASLAALTPVPPCLA
                     LLVSLQPRELGPRAAGPHAAAQDRVIDRAALEQAAGAWLADPWTALVVRTLPNEISKR
                     SRAYGDGGGPAPYFEPDAIGWLLEREVTSLVVDLPSLDRADDGGRLAAHRAYWGLPPG
                     STDAGAASRPHALVTELAFVPDEAQDGLYLLDLQVPAFVADAAPSRPVLYRVVETTRP
                     GPA"
     gene            10164..>10569
                     /locus_tag="VD737_10820"
     CDS             10164..>10569
                     /locus_tag="VD737_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11101.1"
                     /translation="MRLDREHAHALDAADPLAAWHDRFVLPRDPASGRELVYLCGHSL
                     GAQPVLATDYVDEVMRDWRSLGVEGHFSAHHPWMTYHERLAGPLADLVGAAPAEVVAM
                     NTLTVNLNLLLATFYRPAGSRTAILIERHAFPS"
BASE COUNT         1605 a         3753 c         3671 g         1540 t
ORIGIN      
        1 gaccgccgcg caccgcgtcc tggaagtcga ggatgcccgg gttgtgacca ggacacacca
       61 tcaggtttcg cgaatggtag tcgcggtgca cgaacacctg cggctgctcg agcgcattcg
      121 ccacgagcgc atccatggcg gcgtcgagca cccgcgactc atggtccgac aattcgagct
      181 ggaggtgtcg ctgcagcagc cagtcgggaa acaggctcat ctcgaaacgc agcagcttct
      241 cgtcgtaggg cggcagcgtg cgggccgcct ccgccccgcg agcctggatg cgcaccaggg
      301 ccgcgatcgc atccgcgtac agcgggtcgg cgcggcccgg cagcgcgagc tccggcaggt
      361 acatcgtggt gccgaggtcc gtgagcagca ggaatccgtc cccgggcgag cgctcgagca
      421 cgcgcggcgc gttgacgccg cactcctgca gcagtgtcgc gacacgcacg tacggcccga
      481 cgtcttcctt gcccggcggg gcgtccatga cgatcagggg ctcgtgcccg gcgcgcgtca
      541 cgcggaaata gcgacgaaag ctcgcatcgg cggaagccgg cgcgaggcgg tagtcgctga
      601 agccgaggtc ccgctcgagc catcgccgca gcgacgtcaa tcgcgggtcg tgcgcggctc
      661 gttgcgtcag cgtgctcgcg tcgttcaagc ggcaaggtcc tcggggtggt ctgtccgcca
      721 gccgacaaca aagacggcag cggctcggtg tgcgcaatta taatcgggcg aatgtccgtc
      781 ccgccgaccc gatgcagcac tcgatcacgg ttcctgcagc ccgtgcggag caatcgaggg
      841 cagcgcacgg tgggcacggg cgccgtgttc ggcctgcgga cgacgttgct cgcggcctgc
      901 cttgccacgt cagtgccgcg ggtactcgca gccgacgccc ccgcggatga acctgccacg
      961 agcgacgcct gccgggtcgc gacgcccgat ccacgggtcg aagaactgct gcaggccgat
     1021 cctggcgatc cgcgcatcga catcacgagc gacaccggcg agcttggccg cgcgggcgac
     1081 gccgtgctgt ccggcaacgt cacgatccgc accggacagc gactgctgaa ggcggaccag
     1141 gccgaggtcg atgccgaacg acgcagcgtc cggctgcgcg gccgcatgga atacctcgat
     1201 ccgcagctcc acgtcgtcgg cgacagcgga tcgttcatcg aagggggcgg tggccagttc
     1261 aagggcgcgc agttcgagct ggtcgaacgc tccgtgcggg gtgccgccga aggcgcgcgc
     1321 gtcacgcccg acggcaagat ggagctcgag ggcgtgcgct acacggcctg cccgccgggc
     1381 gtcgacgact gggagctgca ggccggtgag atcgcgatcg accagaagac ccagatcggc
     1441 acgggccggg acgtcaagct cgagttcctg ggcctgccga tcttctacac gccctggatc
     1501 acgtttcccg tcggtgacca gcgcaagtcc gggctgctgt ttccgacgat cggcagcagc
     1561 agcaagaccg ggacgctggt cgccgtgccg tactactgga acctcgcgca gaactacgac
     1621 gcgacgctga ccagtcgctg gtactcctcg cgagggtttc gcgtcgatcc ggagctgcgc
     1681 tacctcgacg accgcacccg cagccagctc aacgtcgagt acatgttcca cgacgaggac
     1741 cgcggcgaat cgcgcagcct cgtcgactgg cgccacgtca cgcggctcgc gccgcgcacg
     1801 cggctgctga tcgacgcggc gcacgtgagc gacaccgatt atttcgagga tttcggcgtc
     1861 ggcttcgagg gaaccagtgt cacgtacctc gatcgttacg tggatctgcg ccacgacacc
     1921 ggtccgtggt cgttcacggc cagggcccag ggccaccagg tgatcgacac ggagcttccg
     1981 gaagaagacg agccctacca gatcgtgcct cagctcaccg cgaacggtcg ctggcgcgac
     2041 ctgccaggcg ggctcggcgc ctcgctctac gccgaggcga ccaacttcgc gcgcccgctc
     2101 ggtccggagg gcgcccgctt cgacgccgcg ccctcgctcg agtggcgcat cgaccgcggc
     2161 ggcgggttct tcgctgcaaa cgcgggttac cgctacacgc agtacgcgct gcaggacgtc
     2221 gcgccaggcg tggacgattc gccgagccgg tcgctgccga cggccagcct cgacacgggg
     2281 ctcgtgctcg agcggaccgc gggttcgcgt ggcaaccggt tgcagacggt ggaaccgcgc
     2341 ctgctctacc tccacgtgcc ctaccgcgac caggacgacc tgcccgtgtt cgacaccggg
     2401 ctgccggacc tgaacctcgt gcaactgttc cgcgacaacc gttacgtcgg gcccgaccgg
     2461 gtgggcgacg cgaaccaggt cagcgtcggc gtcacgacgc ggctgctcga ctcgacgcgt
     2521 ggcacgcagt acctgagcgc gacgctcggc caggcgtact acttcgagga cccgcgcgtg
     2581 cgcctgccgg acgagcccgt gcgcgaccgc tcgtcatcgg acgtcgtcgc ggagatcgag
     2641 ctcgcggcgt tcaggaactg gaacgcgcgc tttgcgtacc agtggaatcc cgaccagagc
     2701 cagggtgaac gctcggaaac cttcatccag tacgcgccgg cgccaggccg cgtgttgaat
     2761 gcgggctatc gcttccgccg cgacctgctc gagcaggtcg acgtctcggg ggcctggccg
     2821 atcaacgcgc gctggcgcgg gttcgcgcgc tacgtctatt cgctgcagga agaaaagacg
     2881 ctcgaccagt tcgtcggctt cgagtactcg tcgtgctgct gggccgtgcg actgatcacc
     2941 cggcgcttcg tgtccagccg cagcggcgat gccgagacct cgttcggcct gcaactggaa
     3001 ctcaagggtt tgtcgagtgt cggagtcgac aacgaagctt tcctgcgcga ggcgattcgg
     3061 ggatactccg cgatcccgtc cgctcctcgg acctaggctc cagatgctct tgaaattgaa
     3121 gcagtttttc ggcgtgctcg cgctgctggc ggccgccgcc gggaccgcgc acgcccagtc
     3181 caaggacctg agctcgacgg gcgtgctgct cgaccgcgta gccgcggtcg tcaacgacgg
     3241 catcgtgctg cgcagcgacg tcgagtcaca gatgcaggcg atcagcgagc gcatccagca
     3301 gcagggccag cagctgccgc cgcgcaacgt cctgcgccag caggtgctcg agcgcctcgt
     3361 gctgcaggag ctgcagatgc agcgcgcgga gcgactgggc atcaggatcc ccgacgaggc
     3421 catcaaccag acgctgaccg aggtcgccca gcgcaacaac atcaagttca gcgacctgcc
     3481 cacggcgctc gaatcacagg gcgtcgacta ccgcgactac cgtgacgagg tccgtcgcga
     3541 gctgacgatg cagaccctgc gccagcgcga cgtgcttgcg cgggtgtacg tgtcgccacg
     3601 cgaggtcgag cagtgcatcg ccaagcgcaa ggcttcgccg aacgtcgacg acgagttcaa
     3661 cctgtcgcac atcctggtgg cggtaccgag cacggccgac gagcgccaga tcgccgagcg
     3721 cacctcccgc gcccaggcgg tccacgatcg cgcgaagcag aacgaggact tcgcacagct
     3781 cgcgatcacc tactccgatt caggtaccgc gctcgagggc ggcgccctcg gctggcgcaa
     3841 ggcgagccag ctgccgtcgt tcgtcgcgga catcatcccg aacatgcagg ccggcgacgt
     3901 gacggagccg atccgcacgc cgagcggcct gcacatcttc aaggtgctcg aggtccgtgg
     3961 cgggcaggcg cccgcggtcg tctcgcaggt gcacgcccgt cacatcctga tgaagccgaa
     4021 cgaggtcgag gacgacgaga ccgtgcggca gaagctcacg cagatccgca accgcgtgct
     4081 gggcggcgag agcttcgagg cgatcgcatc ggtcacgtcc gaggatcccg gctcggcggc
     4141 gcagggcggc gacctcggct ggtccggccg tggcagtttc gtgccggaat tcgagcgcca
     4201 gctcgatgcg ctcgaggaga accagatcag cgagccgttc aggaccgaat acggctggca
     4261 catcgtgcag ctgctcggac gtcgcaatta cgacgcgagt ggtgacgtca cccgcaaccg
     4321 ttgcgtcgcg cagttgcgcg aggcacgcgc cgacgaagag acggagatct ggctgcggcg
     4381 cctgcgtgac gaagccttcg tcgaataccg catgtgaacg ccgcgcgacg cccgacccgg
     4441 cggcagccgg gcatgacgct gcggaccgtc gccgtcacga cgggcgagcc ggcggggatc
     4501 ggccctgaca tctgcggcac gctgatcgaa gatccgccgg ccgacagtcg cgtggtgctg
     4561 atcggcgacg gctcgctgat cggccggtcc cgactcaagc gtgcgcacaa gccacggctc
     4621 gcgctgttcg acgccaccgc ggagccgcgg ccggggatcg agctgctgca cgtgccgctc
     4681 gccgccccat gcgtgccggg ccggcccgat ccggccaacg cgcgctacgt gctcgagacg
     4741 ctcgacgccg cgatcggcgg ctgcgcggcg ggcgcgttcc atgcgatcgt gacggcgccc
     4801 gtgcagaagt ccgtgatcaa tgaagcgggc atccccttta ccggacacac tgaatatctc
     4861 gcgcgccaga cgggcgtacg cctgccggtg atgatgctgg tcggggccgg catgcgggtc
     4921 gcgctcgtca cgacccacct ggcgctgcgc aaggtcagcg ccgcgatcac cccggcgcga
     4981 ctggaatccg tgctgcgcat cgtcgagtcc gacctccgca cgatgttcgg cgtgcgcagg
     5041 ccccggttgc tggtgctggg cctcaacccg cacgcgggcg aaggcgggca cctcggtcgc
     5101 gaggagatcg agacgatcga gccggtgctc gccacgctgc gcgcggcagg cctggacctg
     5161 gcagggcccg tgccggccga cacggcgttc gtgccggggc gactcgccgg gttcgacgcg
     5221 gtcgtcgcga tgttccacga ccagggactg ccggtgctca agcacgcgag cttcggacac
     5281 gccgtcaacg tcacgctggg cctgccgatc gtgcgcacct cggtcgatca cgggacggcg
     5341 ctcgaccttg ccggcacgcg caaggcggac accggcagcc tgcgagccgc gttcgacctc
     5401 gcgctggact tgagcagccg cgtgcgcgcg cagacgtcgc actggtgagg tcccgcgcgc
     5461 cctacgcgaa gaagcgctac gggcagcact tcctgcacga cccgggaacc atcgaccgca
     5521 tcgtgcgggc ggtcgatccg cgcccgggcg accggatcgt cgaggtgggg cccggacgcg
     5581 gcgcgatcac ggcgccggtg ctcgaccgcg tgggcgccct cgacgtcgtc gaaatcgacc
     5641 cggacgtcct gcccctgctg caggcgcact gcgcgggccg gggcgagctc cgcgtgcacc
     5701 tcgccgacgc gctcgagttc gatttccggg cgttgcgcgg cgccggcgcg cggctgcggc
     5761 tcatcgggaa cctgccctac aacgtgtcga cacccttgct gttccgcttc atcggacagc
     5821 tcgacgcctt cgcggacatg cacttcatgc tgcagcgtga ggtcgtcgcc cgcatggcgg
     5881 cgcggccgga cacgggcgac tacggccggc tcacggtgac gctggccccg cacgtggccg
     5941 tcgagccgct gttcgacatc ggcaccggcg cgttcaagcc ggcaccgcag gtggtgtcga
     6001 cgttctttgc gctgcgcccg cacgcctcgc ccccgttcga cacgggcgag cctgccgcgt
     6061 atgcgcgcgt ggtgaaggcg gcgttctcga agcgacgcaa gacgctgcgc aacgcactgg
     6121 ccgggctgct gacgcccgag cagatcctgg ccgccggcgt cgaccctgcc gtacgcgccg
     6181 agaccttgcc gcccgagcga ttcggcgcgc tcgcgcgcca gtgccgacca tcgacggcgc
     6241 cggccgattc cggcgcccaa ggtccgtctt aggattgccc tataatcgcc actggctggt
     6301 ggctggtggg gaacgcacgg acacggccgg tcggcggcac tgaccgcagg acactgatca
     6361 ctgacccctc tcccagacgg agcccgacat gcgggaatac aagaagatcc tggcgtgcgt
     6421 cgacctcacg gatggcagcg agcagatcgc cgagcgcgcg cgcgacatgg ccgcacgctg
     6481 cggcggcacg gtcacgctgc tgcacgtcgt cgaatacgtc cccgtcgagc cgatgggcga
     6541 ggcgctgctg ccggccgtgc agatcgaggg cgaactcgtc gaccgcgcaa cgcggcgcat
     6601 cgcggacctc gccgcgcgcg ccgggctcga cacctcggag cgggtcgtgc gcgccggcaa
     6661 catcaagagc gaggtcgtgc ggatcgccca ggaaatcggg gcggacctga tcgtgctcgg
     6721 cagccgcgag cgccacggag tctccatcct gttcaaccag accgaggaca ccgtgctgca
     6781 tgccgcgccg tgcgacgtcc tcgcggtgcg ctggaaccgc aagtgaccga gacggcgtcc
     6841 caccggatcc gcgtcgacgt cgcgaccagc tacgtcgagg accagtcgaa ccccggcgaa
     6901 gcgcgctacg ttttctcgta cacgatcacg atccgcaacg agggcgacaa gcccgcgcga
     6961 ctgatgggcc ggcactggtt gatcaccgac gcaaatggca aggtccagga agtcgtcggc
     7021 gaaggggtgg tcggcgagca gcctcacctg cagccgggcc aggggttccg ctattcgagc
     7081 ggcgccatcc tcgagacgcc cgtggggtcg atgcagggcc gctatcgcat gctggccgac
     7141 gacggcgagc agttcgacgc gccgatcgcc cctttccggc tcgcgatgcc gggcgtcctg
     7201 cactgacctg gcgcgatggc ggtctacgcg atcggcgacg tccagggctg cgccgaccaa
     7261 ctcgaagcac tgctcgtccg gctcgacttc gacgtccacc tcgaccggct ctggttcgtc
     7321 ggcgacctcg tcaaccgcgg gccgcggtcg ctcgacacgt tgcgcctcgt catggcgctc
     7381 ggcgaggccg cgacggtcgt gctgggcaat cacgacctgc acctgctcgc gatcgcccgc
     7441 ggcggcgccc cgtggaagcg tacggacggc ggcctcgagc aggtcatggc ggcaccggac
     7501 cgtgaagccc tgctcgactg gttgcaggcg aagccgctca tgcatcacga cgcggggctc
     7561 caggcgagcc tgctgcacgc cggactgccg ccgcagtggg acgtcgcgac ggcgctggac
     7621 tgcgcgcgcg aagtcgagcg gggcctgcag ggcgagcggg tcggcgaatt gttcgcgcac
     7681 atgtacggca acgagccgtc gctgtggcac gacgacctcg aagggtggga ccgcctgcgc
     7741 ttcacgatca acgccttcac gcggctgcgc gtctgccagg tcgacgacgg gcgcatggtg
     7801 ctcagccaca agggtcctcc cgggacggcg ccccggggcg cggtcccgtg gtttcgggtg
     7861 ccgtggcggc gctcggcggg cgagcggctc gtcttcggcc actggtccgc cctcggttac
     7921 gtcgacgagc agggcgtgct cgggctcgac acgggttgcg tttggggcgg tgcgctgacg
     7981 gcgcagcgga tcgacgccgc agcgtcgcga ccggtgcagg tcaccaacag cgcggacgga
     8041 ctgccgctcg gggattgagc gtcaggaatc cgtggtcgcg ggcgatgatt cgcccgcggc
     8101 gggtgtcggc tccggggtcg tcggcgactc gtcgggcggc ggcagcggct gcagcgtttc
     8161 gagcaccagc cgcaccgccc gtatgggcac gccgtgccgg acgcagccga gttgcgagtc
     8221 ctggcgcagg acccggatgc ccgcgggctc gaggacctgc cgcgcgagtt gctggagcag
     8281 ttcgcagtcg ccgttcgcga tgcgcccgtg ccgcacgccc tcgagctcga cccgctgcca
     8341 cgccgcgggg aactgcgcgg ccgcatcgtc ggccgcgtcg cccctgcccc tcacccgcgc
     8401 gatgagctcg cgctcggcgg caccatcggc gatctgctgc agcagctcgg gagtcgcttc
     8461 gcgcgcgaaa tacgccttga tccgcacgcc cggcatcgct tcgacgccat acccccccat
     8521 ttccacgcag ctggaggaaa cctccaggtc gcgcgcgccc agctgcttca ggatgtagcg
     8581 caccttgtcg cgcaagccgt cgcacgagta ataggtcgtg tcgccgaagt acgtgaacga
     8641 ctgcttgcgc tcgacccaga cggcgttcac gggcggcgtc gcggtggcgg ccggtgcctc
     8701 gctcgccggc tgcgccggga cggcgccaca gccgagaccc agcgccgcga agaccgccgt
     8761 gagcggcgcg gcggtgatgc ttccattcat ggtggctact ctatactcgt tccatgcgag
     8821 tcctggcacc cctgaacctg cggcaatgga tcgacgcgca ccggggcgca ctgcggccgc
     8881 cggtgtgcaa caagcaggtg ttcgacgaag gcgagttcat cgtcatggtc gtgggcggtc
     8941 ccaatgcccg gaaggactat cacgacgacc ccggcgacga gttcttctac cagctcgaag
     9001 gcgacatgct gctgcggacc atccaggacg gccggcgcgt cgacgtgccg atccgcgccg
     9061 gcgaggtgct gttgctgcct gcccacgtgc cgcattcgcc gcagcgattc gccgacacgg
     9121 tcgggctggt ggtcgagcga cggcgacgcc ccggcgaggc cgacggcttc ctgtggttct
     9181 gcgagcgctg cgaccatccg ttgcacagcg agtacctgca ggtgaccgac atcgaggcgc
     9241 agctgccgcc ggtattcgag aggttctggt cgaacccgga gcgcaccgcc tgcgaccgct
     9301 gcggccacgt catgcacaga tgaccgcatg aacgccctcg agttcagcac gcgcggccgg
     9361 cactggcgtg ccgacctgcg ccgcccgatc gacctcgcca tcccgctcga cttcgccggc
     9421 gcccagccgc gcttcttcgc gagcgtcccg gccagcgcag agccgttgcg tgccggctcg
     9481 ttcacgggca gcgtcggatc cggtgcgagc tgcaattgcg ccacgctcac gctggccccc
     9541 cactgccacg gcacgcacac cgagtgcgtc ggccacgtga ccgatccggt ggcctcgctc
     9601 gccgcgttga caccggtgcc gccgtgcctc gcgctgctgg tcagcctgca gccgcgcgag
     9661 ctcggtccga gggccgccgg cccgcatgcc gccgcgcagg atcgggtcat cgaccgggcg
     9721 gcgctcgagc aggcggcggg cgcgtggctc gcggatccgt ggaccgcgct ggtcgtacgt
     9781 accctgccca acgagatttc gaagcggtct cgcgcctacg gcgacggcgg tggccctgcg
     9841 ccctacttcg agcccgacgc catcggctgg ctcctcgagc gcgaagtgac ctcgctcgtg
     9901 gtagacctgc cgagcctcga ccgtgccgac gacggcgggc gcctggcggc gcatcgcgcg
     9961 tactggggat tgccgcccgg atcgacggac gcgggtgcgg cgagccgccc tcacgcgctg
    10021 gtgaccgagc tcgcattcgt gcccgacgag gcgcaggacg gcctctacct gctcgacctg
    10081 caggtccccg cattcgtcgc ggacgctgcg ccgagccgcc ccgtgctgta tcgggtggtc
    10141 gaaacgaccc ggccgggacc ggcatgaggc tcgatcgcga gcacgcgcac gcgctcgacg
    10201 ccgccgaccc gctcgcggcg tggcacgacc gcttcgtgct gccccgcgat cccgcgagcg
    10261 ggcgggagct cgtctacctg tgcggccact cgctcggcgc gcagccggtg ctcgcgaccg
    10321 actacgtcga cgaggtgatg cgcgactggc gttcgctcgg cgtcgagggc cacttcagcg
    10381 cgcaccaccc ctggatgacg taccacgagc gcctggccgg gccgctcgcc gacctcgtcg
    10441 gcgcggcccc ggccgaagtc gtggcgatga acacgctcac ggtcaacctg aacctgctgc
    10501 tcgcgacgtt ctaccgaccc gccggttcgc gcaccgcgat actcatcgag cgtcacgcgt
    10561 tcccgtccg
//
LOCUS       DASPFR010000404         7059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_974986, whole genome shotgun sequence.
ACCESSION   DASPFR010000404 DASPFR010000000
VERSION     DASPFR010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7059
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974986"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VD737_10825"
     CDS             complement(<1..356)
                     /locus_tag="VD737_10825"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11102.1"
                     /translation="MRRILESRPTQVGGGALRGRQELQDRLLDALFELRERKTHAVVM
                     VVTGVPTAGRSEVVNELLSWLDPKQVDVYGFRACNEEELARPPLWRYWRILPPKGRIA
                     ILHGGWHQDLLQQGLAR"
     gene            complement(375..1487)
                     /gene="aguA"
                     /locus_tag="VD737_10830"
     CDS             complement(375..1487)
                     /gene="aguA"
                     /locus_tag="VD737_10830"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: protein motif:HMM:NF010070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HXW11103.1"
                     /translation="MARTLETHPRRDGYRMPGEFERHAGCWMLWPERPDNWRLGAKPA
                     QHAFVALATAIAGSEPVTVGVSAAQYQNARHQLPPQVRVVEVSSNDSWMRDVGPTFVV
                     NDRGGLRGVDWQFNAWGGLDGGLYFPWDRDDAVAQKVLEVEGCDRYRAPFVLEGGAIH
                     VDGQGTLLTTEECLLNRNRNPGLTRTQIESELKRYLAVDTIVWLGRGVHGDETDGHVD
                     NLCAFVRPGEVVLTWTRDRNDPQYEISRDAYERLMEARDARGRRFKVHKLEQPGPLYM
                     TAGEAAGIDAAESSKAREAGGRLAASYVNFYIGNRRVVVPTLDPKRDGKALRQLQELF
                     PRREVVGVPGREILLGGGNVHCVTQQVPLARDRHRS"
     gene            complement(1517..3676)
                     /locus_tag="VD737_10835"
     CDS             complement(1517..3676)
                     /locus_tag="VD737_10835"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HXW11104.1"
                     /translation="MEPDVVTRRAPPMVRLVGFDRRLAADDAAPRPMCCSPTLATAVA
                     AIASAGAGVEDARAAPGDLDPAFGDVGRVSNLGQPTIWSLDVLDDGDVIFGAGDEYCS
                     YWYAGCFPSSGFGRLLASGAPDPAFTAATLQDTTIFDTALQDDGSLVAVGHKLVNDQF
                     RLVVVRLLPDGSLDPQFGADGTQLLPGATMGAEFGRAIQLEDDGRIVVAGTRGEQVLL
                     ARLTADGDLDATFGSGGVTLHGTAVANVVALARTATGYRVVANAALDRPGSFVQTCQV
                     FGVLADGRLDTTFGDAGSGTRTLYVNDFSGQTCDSGGAQLDGRLVVGGGPGPFGDDGF
                     LMRLLVDGTADASFKTTPLADVLPNATAIAFGSGGRIYVAGSRNEGLGGATVVRLLAD
                     GTLDTAYGKDGASDFVLPTGAPVSAVVRDLASGPGGTLLVSGGMPYWSNGFVARLLGD
                     GPGGGPGVLSVVSGGVLGTEGQGQATIAVRRLAGSTGPVSVGYRTRPYSARPSAEEGQ
                     DYAPVSGTLTWADGESGDRDIVVPILSDALTEVPEYFEVELHGADGGAGLGATVSQVH
                     IAGTSYPAGSIQFAAGANSMREGLSQDIQVVRDEYSSGQVSVTVRITGGTAQLDSDYS
                     GADATLTWSDGEMGAKSFRLTGRADREDEGPETITLELVSPTGGAILGALQQSTVSIV
                     DVRRLAAAENGGGHTGALGTTLLGLLAWLRRWRTPQP"
     gene            complement(3798..6929)
                     /locus_tag="VD737_10840"
     CDS             complement(3798..6929)
                     /locus_tag="VD737_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXW11105.1"
                     /translation="MIARAIRGSLANPVLVLLFAAMLTVAGFWALRNTPVDALPDLSD
                     VQVIVRTSFPGQAPRVVEDQVTFPLTTALLAVPGATTVRGYSFFGDSFVYVLFEDGTD
                     LYWARSRVLEYLSQAAARLPEGVRPALGPDATGVGWIYQYALVDRTGRHDLAQLRSIQ
                     DWFLKFELQALDGVAEVATVGGMVKQYQVVVDPLRLRGYGIPLMMVEEAIRKGNQEVG
                     GSVIEMAEAEYMVRATGYVESIADLERIPVMVTDQATPVLLRDVADVRVGPEMRRGIS
                     ELDGQGEAVGGIIVMRSGDNARDTIVRVKAKLDELRAGLPEGVEIVETYDRGSLIQRA
                     VDNLKRKLLDEFIVVAIVCFVLLLHVRSGLIVVIVTPIAVASAFIVMNAQGINANIMS
                     LGGIAIAIGTLVDAAIVMIENVHKKLEHQPDESRRMESIYEGCREVGPSLFFSLMIVA
                     LSFLPVFTLEAQEGRMFAPLAYTKTYAMVAASLLSITLVPVLIWYLVRGRIRPEHANP
                     VNRAAAAAYRPALMLALRRPWAVVVAASVFVLATLWPASQLGSEFMPELDEGDLLYMP
                     TTLPGLSTDAARALLQQTDRLIKTVPEVERVFGKAGRAETATDPAPIEMFETTIMLKP
                     RDQWRPGMTPDKLKAELDGLVKFPGMSNAWGYPIKTRIDMLATGIRTPVGIKIMGPDL
                     QVIQELGQRIEAIVRQVPGTTSIYAERTATGRYVDIDIDRVAAARYGLNIKDVQDIVG
                     SAVGGMTVSYTVEGLERYPINLRYPQEFRDSLEKLKQLPIVAPTGANVTLQDVARVGI
                     ADGPGMIRSENARPSGWVFVDLQGRDLGGFVEEARSRIAEQLELPAGYSLSWSGQYEY
                     LARAVERLKVVVPATLAIIVLLLYLNFRNGRDVGLILFALPFALVGGIWLLYLLDYDL
                     SIAAAVGFIALAGVAAETGVVMVMYLEHAWAQRRARAAAEGRAPTRPELREAIVEGAL
                     LRLRPKLMTVITIIVGLLPIMWGSGTGSEMMRRIAAPMIGGMVSATILTLVIIPSLYL
                     IVNGWRLRDEREA"
BASE COUNT         1097 a         2519 c         2344 g         1099 t
ORIGIN      
        1 cgtgcgagcc cctgctgcag gaggtcctga tgccagccgc cgtgcaggat cgcgatgcgg
       61 cctttcgggg gcaggatgcg ccagtagcgc cacagcggcg gacgcgcgag ttcctcctcg
      121 ttgcacgccc ggaaaccgta gacgtccacc tgcttgggat cgagccagct caggagctcg
      181 ttgaccacct cgctgcgccc ggccgtcgga acgccggtga ccaccatgac cacggcgtgc
      241 gtcttgcgtt cgcgaagttc gaacagcgcg tcgagcaggc ggtcctgcag ctcctgccgt
      301 ccccgcaggg ctccgccgcc gacctgcgtc ggacggcttt ccagaatccg tcgcaaccgt
      361 cgtcgagtca tggctcagga ccggtggcga tcgcgggcga gcggtacctg ctgcgtcacg
      421 cagtgaacgt tgccgccacc gagcaggatt tcgcgccctg gcacgccgac gacctcgcgc
      481 ctcgggaaca attcctgcag ctgccgcagc gccttgccgt cgcgcttcgg gtccagcgtc
      541 ggcaccacca cacggcggtt gccgatgtag aagttcacgt acgaggcggc cagccgtccc
      601 ccggcctcgc gcgccttgct gctttccgcc gcgtcgatgc cggcggcctc cccggccgtc
      661 atgtacagcg gccccggctg ctccagcttg tggaccttga agcgccggcc gcgcgcgtcg
      721 cgagcctcca tgagccgctc gtaggcgtcg cgcgagatct cgtactgggg atcgttgcga
      781 tcacgcgtcc acgtcagcac gacctctccg ggccgcacga acgcacagag gttgtcgacg
      841 tggccgtcgg tctcgtcgcc gtgcacgcca cggcccagcc agacgatcgt gtcgacggcg
      901 agatagcgct tgagctcgga ctcgatctgg gtgcgcgtga gtcccggatt gcgatttcgg
      961 ttgagcaggc actcttcggt cgtgagcagc gtgccctgcc cgtcgacgtg gatcgcgccg
     1021 ccttcgagca cgaacggcgc ccggtagcgg tcgcagcctt ccacttccag caccttctgg
     1081 gcgacggcat cgtcacggtc ccaggggaag tagaggcccc cgtcgagtcc gccccacgcg
     1141 ttgaattgcc agtcgacgcc ccgcaggccg ccgcggtcgt tgacgacgaa cgtggggccc
     1201 acgtcgcgca tccaggagtc gttgctcgag acttccacca cgcgtacctg cggcggcaac
     1261 tggtggcgtg cgttctggta ctgcgcggcc gagacgccca ccgtcacggg ctcgctgccc
     1321 gcgatcgccg tggcgagcgc gacgaaggcg tgctgcgcag gcttggcgcc gagccgccag
     1381 ttgtcgggcc gctcgggcca cagcatccag cagccggcgt gccgctcgaa ctctccgggc
     1441 atccggtagc cgtcgcggcg cgggtgagtc tcgagggtgc gtgccatccc gcgatggtaa
     1501 caaagcgctc agcgcgtcaa ggctgcggcg tcctccagcg gcgcaaccac gcgagcagac
     1561 cgagcagcgt cgtgccgagc gcgcccgtgt ggcctccacc gttctcggcg gcggccaatc
     1621 tcctcacgtc gacgatggac acggtcgatt gctgaagggc gcccaggatg gctccaccgg
     1681 tgggcgagac aagctcgagc gtgatcgtct cgggcccttc gtcctcccgg tccgcgcgtc
     1741 ccgtgagtcg aaaactcttc gcgcccattt cgccgtccga ccacgtcaag gtcgcgtccg
     1801 cgccactgta gtcgctgtcc aattgtgcgg tgccgcccgt gatgcgcacc gtcaccgaga
     1861 cctgaccact gctgtattcg tcacgaacga cctggatgtc ctgcgacaac ccttccctca
     1921 tcgaattggc gccagcggcg aactgaatcg aaccggcggg atacgacgtc cctgcgatgt
     1981 ggacctggct cacggtcgcg ccgaggcctg cgccgccgtc cgcgccgtgc agttccacct
     2041 cgaagtactc ggggacttcc gtcagggcgt cggacaggat cggcacgacg atgtcacgat
     2101 cgcccgactc gccgtccgcc caggtcaacg tgcctgacac gggcgcgtag tcctgcccct
     2161 cctccgcgga cggacgagcc gagtaaggac gcgtgcgata accaacgctg accggcccgg
     2221 tgctgcccgc gagccggcgg acggcgatcg tcgcctggcc ctgtccttcc gtgccgagga
     2281 cgccgccgct cactacggag agcacgccgg ggccaccgcc agggccgtcc cccagcaggc
     2341 gagcgacgaa tccattcgac cagtacggca tcccgccgga caccagcagc gtgccaccgg
     2401 ggccggatgc aaggtcgcgg acgacggcgg acaccggcgc acccgtgggg agcacgaaat
     2461 cgctggcacc gtccttgccg tacgcggtgt cgagcgtgcc atcggcgagc agccggacca
     2521 cggtggcgcc gcccaggcct tcgttgcgcg agcccgccac gtagatgcgc ccgccgctgc
     2581 cgaacgcgat cgccgtggcg ttcggcagca catcggcgag cggcgtcgtc ttgaatgagg
     2641 cgtccgcggt gccatccacg agcaatcgca tcaggaatcc gtcgtcaccg aacggcccgg
     2701 ggccgccgcc gacgaccagc cgaccgtcca gctgggcccc gccgctgtca caggtctgcc
     2761 cgctgaagtc gttcacgtag agcgtcctcg tgccgctgcc ggcgtcgccg aacgtcgtgt
     2821 ccagccgtcc gtccgccagt acgccgaaca cctggcacgt ctgcacgaag ctgccaggcc
     2881 ggtccagcgc cgcattggcc accacccggt agccggtcgc ggtgcgggcg agcgcgacca
     2941 cgtttgcgac ggcagtgcca tgcaacgtca cgccgccgct accgaaggtc gcgtcgagat
     3001 cgccgtcggc cgtcagtcgc gcaaggagca cctgctcgcc tcgggtgccg gccacgacga
     3061 tgcggccgtc gtcctcgagc tggatcgcgc ggccgaattc tgcacccatc gtcgcgcctg
     3121 gcagcagttg ggtgccgtcg gcgccgaact gcggatcgag cgaaccgtcg gggagcaacc
     3181 gcacgaccac gagccgaaac tggtcgttga cgagcttgtg cccgaccgcg accaggctgc
     3241 catcgtcctg cagggccgtg tcgaagatgg tcgtgtcctg cagcgtcgct gcagtaaagg
     3301 ccggatcagg cgcgccgctc gccagcagtc gcccaaaccc ggagctgggg aaacaacctg
     3361 cgtaccagta gctgcagtac tcgtcgcccg cgccgaagat gacgtcgccg tcgtccagga
     3421 cgtcgagcga ccagatggtc ggttggccga ggttggacac ccggccgacg tccccgaacg
     3481 ccgggtccag gtcaccgggc gcggcccggg cgtcttcgac accggctcct gcactcgcga
     3541 tcgccgcgac ggcggtcgcc agcgtcgggc tgcagcacat cggtcgcggt gctgcatcgt
     3601 cggcggcgag gcgccgatcg aatccgacga gcctcaccat gggcggcgca cggcgcgtta
     3661 cgacgtccgg ttccatggcg atcctccctt ccttcttgct tttcgttgct gcggacggcc
     3721 tgcagcccgc tcggctgatg gttgcaccga gcgcgccgga agtcctgaag ttgtctgaac
     3781 atttgtgaag acggcggtca ggcctcccgc tcgtcgcgca accgccagcc gttcacgatc
     3841 aggtagagcg acgggatgat caccagcgtc aggatggtcg ccgacaccat cccgccgatc
     3901 atcggtgccg cgatgcggcg catcatctcg gaccccgtgc cgctgcccca catgatcggc
     3961 agcaggccga cgatgatggt gatgacggtc atgagcttgg gccgcagtcg cagcagcgcg
     4021 ccctcgacga tcgcctcgcg cagttccggc cgggtcggcg cccgtccctc cgccgccgcg
     4081 cgcgcgcggc gctgcgccca ggcgtgctcg aggtacatga ccatcaccac gcccgtttcc
     4141 gcggcgacgc ccgccagggc gatgaagccc acggcggccg cgatcgagag gtcgtaatcg
     4201 agcaggtaga gcagccagat gcccccgacc agcgcgaacg gcagcgcgaa caggatcagc
     4261 ccgacgtcgc gcccgttgcg gaagttgagg tacagcagca ggacgatgat cgccagcgtt
     4321 gcggggacga ccaccttgag gcgctcgacg gcgcgggcca ggtattcgta ctggccggac
     4381 cagctcaggc tgtagccggc cgggagctcg agctgctccg cgatgcgcga cctggcttcc
     4441 tcgacgaagc cgcccaggtc gcgcccctgc aggtcgacga acacccagcc ggatggccgc
     4501 gcattctcgc tgcggatcat gccagggccg tcggcgattc cgacgcgtgc gacgtcctgg
     4561 agcgtcacgt tggcgccggt cggggccacg atcggcaact gcttgagctt ctccagcgag
     4621 tcgcggaact cctgcgggta gcgcaggttg atcggatacc gctcgagtcc ctcgaccgtg
     4681 tagctgaccg tcatgccgcc gaccgcgctg ccgacgatgt cctgcacgtc cttgatgttg
     4741 agcccgtagc gcgcggccgc gacgcggtcg atgtcgatgt cgacgtagcg gccggtcgcc
     4801 gtgcgctccg cgtatatcga agtggtcccc ggcacctgtc tcacgatcgc ctcgatccgc
     4861 tgcccgagct cctggatcac ctgcaggtct ggacccatga tcttgatgcc gaccggcgtg
     4921 cggatgcccg tggcgagcat gtcgatgcgc gtcttgatcg ggtagcccca ggcgttcgac
     4981 atgccgggga acttcacgag cccgtcgagc tcggccttga gcttgtccgg cgtcatgccc
     5041 ggccgccact gatcgcgcgg cttgagcatg atcgtcgttt cgaacatctc gatcggggcg
     5101 ggatcggtcg cggtttccgc acgccctgcc ttgccgaaga cgcgctcgac ctcgggcacg
     5161 gtcttgatca gccggtccgt ctgctgcagc agcgcgcgcg ccgcgtccgt ggacaggcct
     5221 ggcagcgtgg tcggcatgta cagcaggtcg ccctcgtcga gctccggcat gaactcggag
     5281 ccgagctgcg acgcgggcca gagcgttgcc aggacgaaca cgctcgccgc caccaccacg
     5341 gcccacggcc gtcgcagcgc cagcatcagc gcgggccggt aggccgccgc cgcggcgcgg
     5401 ttgaccgggt tcgcgtgctc ggggcggatg cgcccgcgca cgaggtacca gatcagcacc
     5461 ggcacgagcg tgatcgacag cagcgacgcc gcgaccatcg cgtaggtctt ggtgtaggcg
     5521 agcggcgcga acatgcgccc ctcctgcgcc tcgagcgtga acaccggcag gaacgagagc
     5581 gccacgatca tcagcgagaa gaacagcgac ggtccgacct cgcggcagcc ctcgtagatc
     5641 gactccatgc gtcgcgactc gtcgggctgg tgctcgagct tcttgtgcac gttctcgatc
     5701 atcacgatcg cggcatcgac cagcgtgccg atcgcgatcg cgatgccgcc cagcgacatg
     5761 atgttggcgt tgatgccctg cgcgttcatc acgatgaatg ccgaagccac ggcgatcggc
     5821 gtgacgatga cgacgatcag gcccgagcgc acgtgcagca ggagcacgaa gcagacgatc
     5881 gccacgacga tgaactcgtc gagcagcttg cgtttcaggt tgtcgaccgc gcgctggatc
     5941 agcgagccac ggtcgtaggt ctcgacgatc tcgacgccct cgggcagccc tgcgcgcagc
     6001 tcgtcgagct tcgccttgac gcgcacgatg gtgtcgcgcg cgttgtcgcc gctgcgcatg
     6061 acgatgatgc cgcccacggc ctcgccctgt ccgtcgagtt cgctgatgcc gcggcgcatc
     6121 tcggggccga cgcgcacgtc ggcgacgtcg cgcagcagca ccggggtcgc ctggtcggtc
     6181 accatcaccg ggatgcgttc gagatcggcg atgctctcga cgtagccggt cgcgcgcacc
     6241 atgtactcgg cctcggccat ctcgatgacc gagccgccga cctcctggtt gcccttgcgg
     6301 atcgcttcct cgaccatcat cagcggaatg ccgtatccgc gcagtcgcag cgggtcgacg
     6361 acgacctggt actgcttgac catcccgccg accgtcgcca cctcggcgac gccgtcgagc
     6421 gcctgcagct cgaacttcag gaaccagtcc tggatcgagc gcagctgcgc gagatcgtgg
     6481 cggccagtgc ggtcgacgag cgcgtactgg tagatccagc cgacaccggt tgcgtcgggt
     6541 cccaaagccg ggcgcactcc ctcgggcagc cgtgcagccg cctggctcag gtactcgagc
     6601 acgcgggagc gcgcccagta gagatcggtg ccgtcctcga acagcacgta gacgaacgag
     6661 tcgccgaaga acgaataccc gcgcacggtc gtcgcaccgg gcaccgccag gagcgccgtc
     6721 gtcagcggaa aggtcacctg atcctcgacg acccgtggcg cctggcccgg gaagctcgtt
     6781 cggacgatca cctgcacgtc gctcagatcg ggcaatgcat ccaccggcgt gttgcgcagc
     6841 gcccagaagc ccgcgaccgt gagcatcgcc gcgaacagca gcacgagcac cggattcgcc
     6901 agcgatccgc ggatcgcccg cgcgatcagc ccatcctgat gagtgtgcat gacgacctca
     6961 gtgctggtgc tcgacgtccg cggactcgcc cgtcccggtg aagcgcgcga ggctcgagcg
     7021 cagactcgct tccgaatcga tcaggaacac tcccgaggt
//
LOCUS       DASPFR010000405         3832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_1464180, whole genome shotgun sequence.
ACCESSION   DASPFR010000405 DASPFR010000000
VERSION     DASPFR010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464180"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2273
                     /locus_tag="VD737_10845"
     CDS             <1..2273
                     /locus_tag="VD737_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11106.1"
                     /translation="ISADLLAGSRDTILSEFYERALADQPPGVRRVVEDELLTESGYR
                     ESLAEERVLKALAAAGAPAEALGTLVDRRLLRIEERLDVRRVELTHDVLCSVVKQSRD
                     VRHEREARDAAERQLAAQRDRAAETRRTLHRTRRFAIAAGVLMLVALAGAAFGWINWQ
                     RALAADEKTQQARADAEKLVGFLIEDFYEELEPTGRLETMGKLAHMAVSYYDGLPKEL
                     LTPQTQIYRGMALIREGSARLARGDHEKGLPHLNEAREVFTQLRQAGQDTEPVKLGLA
                     LSQFARFSAWGVNGAPGSSTADLQQAADLLRPLARSPDGPRNIKLVYADVLNYLSHRQ
                     PKSVGVASCEEARSLLASMGARDLTDLRAASIYADTSDSEARHLVALGRIEDAERLTR
                     EVYDIAEKVLTQRPGDLRSMANRVLAADVLSRLAGRRYDYPVAAEYAARAERAGEDFV
                     RFNPSDMGAWQYWINGLDLGADVLLEQGRVREAEAAWRRTVGLEDDPRKPSSLATVLW
                     QVWPKLAVLQARSGAPVEAAATLHEGAKAVLELASLEEEGSPRRTLFNLTADAWRARI
                     SLWNGHSTGARDSAHALAVTIRAVPTPASDPNLVAIRANYLRGVLATEALAALRVGDL
                     RAAEAAARERRELPPNPFSELDPQDEVSRSQVILAHAIVKQGRDDEALALVRDEVIRY
                     RDKHKAGATGLSFSIDFAHALYVAALAQSDGRRRGALLTEASGLLHGLPTETRQFADV
                     QQLLAWIESARAASAA"
     gene            complement(2286..3713)
                     /locus_tag="VD737_10850"
     CDS             complement(2286..3713)
                     /locus_tag="VD737_10850"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HXW11107.1"
                     /translation="MDISKARGFIDQAWSDSILPQLVDYVRIPNKSPIFDRDWEAHGH
                     MEQAVQLMQRWAKAEAPAGSTIEVLRIPGRTPVLMVDVPGDVDDCVLMYGHLDKQPEF
                     TGWSDGLEPWTPVLRDGKLYGRGGADDGYALFSSIAAIRALREQRVPHARCVVLIEAS
                     EESGSPDLPAHIDALGARLGTPSLVVCLDAECGNYDQLWCTTSLRGNLIGTLRVDVLR
                     EGVHSGTASGVVPSSFRLLRELLARVEDAHSGAVLVEELNTAIPADRRAQAQAAAAVL
                     GETVVGKFPLVAGMRPVCNDPFELLLNNTWRPTLSVTGVEGIPPFHSAGNVLRPYTAV
                     KLSFRLTPTTDPKLASAAIKRSLESDPPYGAQVTFRVESAMGGWNAPSFAPWLEQSMR
                     DASQAFFGRESMYMGTGGSIPFMGMLGEKFPGTQFLITGVLGPHSNAHGPNEFLHLDT
                     ARKVTGCVAKVLADHATRATQRRAA"
BASE COUNT          611 a         1316 c         1323 g          582 t
ORIGIN      
        1 agatctccgc cgacctgctc gcgggttcgc gcgacaccat cctgtccgag ttctacgagc
       61 gcgccctggc ggaccagccg cccggcgtcc gccgcgtggt cgaggacgag ttgctgaccg
      121 agtcgggcta tcgcgagagc ctggccgaag agcgcgtgct gaaggcgctt gccgcggcgg
      181 gagcgccggc cgaggcgctc gggacgctgg tcgaccggcg cctgctgcgc atcgaggagc
      241 gtctcgacgt acggcgcgtc gagctgacgc acgacgtgct ctgcagcgtc gtcaagcaaa
      301 gccgcgacgt gcggcacgaa cgcgaggcgc gggacgcggc ggaacggcag ctcgccgcgc
      361 agcgggaccg cgccgccgag acgcggcgca cgctgcaccg cacccgtcgt ttcgccatcg
      421 cggccggtgt gctcatgctc gtggccctgg cgggggccgc cttcggctgg atcaactggc
      481 agcgggcgct cgcggccgac gagaagacgc agcaagcccg cgccgacgcc gaaaagctcg
      541 tcggctttct gatcgaggac ttctacgagg agctcgagcc caccggccgg ctcgagacca
      601 tgggcaagct cgcgcacatg gccgtgagct attacgacgg gctgccgaag gaactgctga
      661 ccccgcagac gcagatctat cgaggcatgg cgttgatccg ggagggaagc gcgcgcctcg
      721 cgaggggcga tcacgaaaag ggcctcccgc acctgaacga agcccgcgag gtgttcactc
      781 agctgcgaca ggccgggcag gataccgaac cggtcaagct cggactcgcg ttgtctcagt
      841 tcgcccggtt ctcggcatgg ggcgtcaacg gtgccccggg ctcgagcacc gccgacctgc
      901 agcaggcggc cgatctgttg cggccgctcg cgagatcccc cgacggaccg cgcaacatca
      961 agctcgtcta cgcggacgtg ctcaattacc tgagtcaccg ccagcccaaa tcggtcgggg
     1021 ttgcgagctg cgaggaggcc cgcagcctgc tggccagcat gggggcgcgc gatcttaccg
     1081 acctccgggc ggcctcgatc tacgccgaca ccagcgattc ggaggcccgg cacctcgtgg
     1141 ccctcggtcg catcgaggac gcggaacgac tgacccgcga ggtctacgac atcgcggaaa
     1201 aggtcctgac acaacggccc ggcgacctgc gctcgatggc aaatcgcgtc ctggcggccg
     1261 acgtgctgag ccgcctggcg gggcgtcgat acgattaccc cgtggcggcg gagtatgcgg
     1321 cgcgcgccga gcgtgccggc gaggacttcg tgcgcttcaa cccgtccgac atgggtgcct
     1381 ggcagtattg gatcaacggg ctggacttgg gcgccgacgt gctgctcgaa caggggcgcg
     1441 tccgggaagc cgaggcggcg tggcggcgaa ccgtgggcct ggaggacgat ccccgcaaac
     1501 cgtcgagtct cgccaccgtg ttgtggcagg tgtggccgaa gctcgcggtg ctgcaggccc
     1561 gttccggcgc accagtcgag gcagccgcca cgctgcatga aggcgccaag gcggtcctcg
     1621 agctggcctc gctcgaggag gagggcagcc cgcgacgcac gctcttcaac ttgaccgccg
     1681 atgcctggcg cgctcgcatc tcgctctgga atggccactc cacgggcgcc cgcgactccg
     1741 cgcacgcgct tgccgtgacg atcagggcgg tgccgacgcc ggcgagcgat ccgaacctcg
     1801 tcgcaatccg cgcgaactac ctgcgggggg tgctcgccac ggaggccttg gctgccctcc
     1861 gcgtggggga cctgcgcgcc gcggaggccg ccgcacgcga acggcgcgaa ctgccgccga
     1921 atccgttctc cgagctcgac ccgcaggacg aggtgtcgcg ttcacaggtc atcctggccc
     1981 acgcgatcgt gaagcagggc cgtgacgacg aggcgctcgc gctggtccgc gacgaagtga
     2041 tccgctaccg cgacaaacac aaggccggtg cgaccggcct gtcgttctcg atcgatttcg
     2101 cccacgcgtt gtacgtggcc gccctcgccc aatccgacgg caggcggcgc ggcgcgttgt
     2161 tgaccgaggc gtccggcttg ctgcacgggc tgccgaccga gacccggcag ttcgcggacg
     2221 tccagcagct gctcgcatgg atcgagtcgg cgcgggctgc atccgccgct tgaggaggcg
     2281 acgactcagg cagcccggcg ctgcgtcgcc cgcgtggcgt ggtccgcgag caccttggcc
     2341 acgcaccccg tgaccttgcg cgccgtgtcg aggtgcagga actcattggg cccgtgcgcg
     2401 ttggaatggg gacccagcac gccggtgatc aggaactggg tgcccgggaa cttctccccg
     2461 agcatgccca tgaagggaat gctgccgccc gtgcccatgt acatggactc gcggccgaag
     2521 aatgcctgcg acgcgtcccg catcgactgc tcgagccacg gcgcgaacga cggtgcgttc
     2581 cagccgccca tcgccgactc gaccctgaac gtgacctgcg cgccgtaggg cggatcgctc
     2641 tcgagtgacc gcttgatcgc ggcgctcgcc agtttcggat ccgtcgtcgg ggtgagccgg
     2701 aacgagagct tgaccgcggt gtacggacgc agcacgttgc ccgcgctgtg gaacggcgga
     2761 atgccctcga ccccggtcac gctcagcgtc ggtcgccatg tgttgttgag cagcagttcg
     2821 aacgggtcgt tgcacacggg ccgcatgccc gcgaccaggg gaaacttgcc aaccacggtt
     2881 tcgcccagca ccgctgcggc agcctgggct tgcgcgcgcc ggtcggcggg gatcgccgtg
     2941 ttgagttcct cgaccagcac ggcgccggag tgcgcgtcct cgacgcgcgc gagcagttca
     3001 cgcaagagtc ggaagctcga cggcacgacg ccgctcgccg tcccggaatg cacgccttcg
     3061 cgcagcacgt ccacccgcag ggtgccgatc aggttgccgc gcagcgacgt cgtgcaccag
     3121 agctggtcgt agttgccgca ctcggcgtcg aggcagacga cgaggctcgg ggtgccgagc
     3181 cgcgcgccga gcgcgtcgat gtgggccggg aggtcggggc tgccgctttc ctcgctcgcc
     3241 tcgatcagga ctacacagcg tgcatggggc acccgctgct cgcgcagcgc ccggatcgcc
     3301 gcgatggacg agaacagggc atagccgtcg tcggcgccgc ctcggccata cagcttcccg
     3361 tcgcgcagca ccggggtcca cggctcgagg ccgtccgacc agccggtgaa ctccggctgc
     3421 ttgtcgaggt ggccgtacat gagcacgcag tcgtcgacgt cccccggcac gtcgaccatc
     3481 agcaccggcg tgcgtcccgg gatgcgcagg acttcgatcg tcgaacccgc cggcgcctcg
     3541 gcctttgccc aacgctgcat cagctgcacg gcctgctcca tgtggccgtg cgcttcccag
     3601 tcgcgatcga agatcggcga cttgttcggg atccgcacgt agtcgaccaa ctgcggcagg
     3661 atcgaatccg accaagcctg atcgatgaaa cctcgagctt tcgaaatgtc catatatatc
     3721 aatgtcttgg tgagatcttt gaataaacag tgtctagtgt ctcgtgtcta gtgtttagta
     3781 ggaatcggca ggtcggactt acggtcccta ctaaccgcta gacactgaac ac
//
LOCUS       DASPFR010000406         2880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate SMAG_U16429
            k141_732385, whole genome shotgun sequence.
ACCESSION   DASPFR010000406 DASPFR010000000
VERSION     DASPFR010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935425
            Sequence Read Archive: SRR25158316
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (soil metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:07:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,173
            CDSs (total)                      :: 2,155
            Genes (coding)                    :: 2,140
            CDSs (with protein)               :: 2,140
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 15
            CDSs (without protein)            :: 15
            Pseudo Genes (ambiguous residues) :: 0 of 15
            Pseudo Genes (frameshifted)       :: 1 of 15
            Pseudo Genes (incomplete)         :: 13 of 15
            Pseudo Genes (internal stop)      :: 2 of 15
            Pseudo Genes (multiple problems)  :: 1 of 15
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732385"
                     /isolate="SMAG_U16429"
                     /isolation_source="soil"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /country="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /locus_tag="VD737_10855"
     CDS             <1..1428
                     /locus_tag="VD737_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXW11108.1"
                     /translation="GEPAADPAQGLGRRLARTLREGVIETVDDESYRRADGSTIPVTY
                     STHPMVRDGRIVGAVVSFMDATERRALERARESALHEAERLAQARSEFLANMSHEIRT
                     PLNGVLGLAQVAHRDPAVEASTREIFGRILEAGRTLLGVVDDVLDFSRIEAGKVQVEA
                     VPADLRELVEEVMTSIRPVAAAKGLELRVEVTPGVPQRCATDPIRVKQVLLNLLANAV
                     KFTERGHVALRVSREDGRLQLVVRDTGIGIRDDQASRIFSAFEQADTSTTRRFGGTGL
                     GLAICKRIADLMGGDITVRSQVGVGSEFTVTLPCVVADDASCREGDERSASATRRVER
                     LAGLRLLLVEDNEVNQFVARAMLSREGPTVVMASDGRQAVECIRREGPGAFDLVLMDI
                     QMPVMDGYEATRLVHAIDPDLPVIGQTAHVLQEAIDQCRAAGMVAHLPKPLDRHELLE
                     AIMRHARGTLRGPAETPRAAAGVSV"
     gene            complement(1379..1975)
                     /locus_tag="VD737_10860"
     CDS             complement(1379..1975)
                     /locus_tag="VD737_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW11109.1"
                     /translation="MTYEICWKLAYTVFVAIVVAIWLRHYGWRNLLWFSDIALIGAVP
                     ALWLESSAIASVLAVAVLVPELLWNVDFGLRLLLRRRITGLTDYMFERERPRMLRVVS
                     ALFHVPLPILLLWMLAEYGYDGAAGLPGAVLLAAVVLPWSRAVSSPERNINWTHGLGP
                     IRTRLPAQRYVLQLFVGFVLLVFIPTHLLLRAVFPPGP"
     gene            2128..2502
                     /locus_tag="VD737_10865"
     CDS             2128..2502
                     /locus_tag="VD737_10865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HXW11110.1"
                     /translation="MNAQRFHKGMIGSMIAFVGTFALAASVHAGDVAPDAAPAVKHDD
                     VVVRYADLNLASEEGAEALYARLSAAAARACGNTPMAWEQKARREYKACVDRKLEKAV
                     GKVGNSKVSAVHAVRKEAAKVG"
BASE COUNT          514 a          978 c         1004 g          384 t
ORIGIN      
        1 ggcgaacccg ccgcggaccc ggcccagggg ctcgggcggc gcctcgcgcg cacgctgcgg
       61 gaaggcgtca tcgaaacggt cgacgacgaa agttaccgcc gcgccgacgg gagcacgatc
      121 ccggtcacct attcgacgca ccccatggtg cgcgacgggc gcatcgtggg tgcggtcgtg
      181 agtttcatgg acgccacgga acggcgtgcg ctcgagcgcg cgcgcgagtc cgcgttgcac
      241 gaggccgagc ggctcgcaca ggcacgcagc gaattcctcg cgaacatgag ccacgagatc
      301 cgtacgcccc tcaacggagt gctcggcctg gcgcaggtcg cgcaccgcga cccggccgtc
      361 gaggcgagca cgcgcgagat cttcggacgc atcctcgagg cgggccgcac gctgctcggc
      421 gtcgtcgacg acgtgctcga tttctcgcgg atcgaagccg gcaaggtgca ggtggaggcc
      481 gtgccggcgg acctgcgcga actcgtcgag gaagtcatga cgtcgatccg ccccgtggcg
      541 gcagccaagg ggctcgagct gcgcgtcgag gtcacgccgg gagtgccaca gcgctgcgcc
      601 accgacccga tacgcgtcaa gcaggtgctg ctcaacctgc tggccaatgc cgtcaagttc
      661 accgagcgtg gccacgtggc gctgcgcgtc tcgcgcgagg acggcaggct gcagctggtc
      721 gtgcgtgaca ccggcatagg catccgtgac gaccaggcgt cgcggatctt ctccgcgttc
      781 gagcaggccg acacgagcac cacgcgccgc ttcggcggca cgggactcgg cctcgcgatc
      841 tgcaagcgga tcgcggacct gatgggcggg gacatcacgg ttcgcagcca ggtcggcgtc
      901 ggcagcgagt tcaccgtgac gctgccgtgt gtcgtggccg acgatgcctc gtgccgggag
      961 ggcgacgagc gcagtgccag cgccacgcgc cgcgtcgagc gactggcggg gctgcgattg
     1021 ctgctggtcg aggacaacga ggtcaaccag ttcgtggcgc gggcgatgct gagccgggaa
     1081 ggtcccaccg tcgtcatggc gtccgacggt cgccaggccg tcgagtgcat ccgccgcgaa
     1141 ggccccggtg ccttcgacct ggtgctgatg gacatccaga tgccggtcat ggacggctac
     1201 gaggcgacgc gcctcgtgca cgcgatcgat ccggacctgc cggtgatcgg ccagaccgcg
     1261 cacgtgctgc aggaggccat cgaccagtgc cgcgccgcag gcatggtcgc ccacctgccg
     1321 aagccgctcg accgccacga gttgctcgag gcgatcatgc ggcacgcccg cgggacgctc
     1381 aggggcccgg cggaaacacc gcgcgcagca gcaggtgtgt cggtatgaag acgagcagca
     1441 cgaacccgac gaacaactgc agcacgtagc gctgcgccgg caggcgggtc cggatcgggc
     1501 caagcccatg cgtccagttg atgttgcgct ccggtgaact gaccgcccgg ctccagggca
     1561 gcaccaccgc tgcgagcagc accgcccccg gcaagcccgc ggcgccgtcg taaccgtact
     1621 cggccagcat ccagagcagc aggatcggca ggggcacgtg gaacagcgcg gacaccacgc
     1681 gcagcatcct cggccgctcg cgctcgaaca tgtagtcggt cagcccggtg atccggcgcc
     1741 gcaacagcag tcggagcccg aagtcgacgt tccagagcaa ctcgggcacc agcaccgcga
     1801 ctgccagcac gctcgcgatc gccgaactct cgagccagag cgccggcacc gcgccgatca
     1861 gcgcgatgtc ggagaaccag agcaggttgc gccatccgta atgacgcagc cagatcgcca
     1921 cgacgatggc gacgaatacg gtgtaggcga gcttccagca gatctcgtac gtcacgttcc
     1981 actcacctta ccagacgcga ctgcgacgaa gcgccggacg cgcgcgacga agcgccgaac
     2041 atgcgacctc cgttcgcatc atattgatca cacggtgagt gaaagaacga acgacgcacg
     2101 agagttgaca cacaggagca agacaagatg aacgcccagc gcttccacaa gggaatgatc
     2161 ggatcgatga tcgctttcgt gggcaccttc gccctcgccg catcggtgca cgccggtgac
     2221 gtggcccccg acgccgcgcc ggccgtgaag cacgacgacg tcgtcgtgcg ttacgcagat
     2281 ctcaacctcg cctcggaaga aggcgccgag gcgctgtacg cgcggctctc cgccgctgcg
     2341 gcacgtgcct gcggcaacac gccgatggcc tgggaacaga aggcacgcag ggaatacaag
     2401 gcctgcgtcg accgcaagct ggagaaggcg gtgggcaagg tcggcaactc caaggtttcg
     2461 gcggtgcacg ccgtccgtaa ggaagcagcg aaggtcggct gacgttcctg caacgacagg
     2521 gacgtcctgc gaggcgggtc gtgaccggcg acccgcctcg ccccccatcc gcgcgcgatc
     2581 tcgtcgcgct gctgcggccg gcctggtccg ttcaggacga cctgtgaaga ccgtcgcgag
     2641 ccacggatgg cgagcgcgag cccccaggga cgggtttacg gcgtgtcttc cgtcgcactc
     2701 cggaaggtgc cgggccggtc gtgctgccgc ccaagtgatc gccgcagcga gtgacggaaa
     2761 gccagcgagg ctaatccatg ggtgcgatct gcgcgacgag attgcgcgcg gatgggggcg
     2821 aggcgggtcg ccggtcacga cccgcctcgc aggacgtccc tgtcgttgca ggaacgtcag
//
